LOCUS       DAJTUN010000001        77979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTUN010000001 DAJTUN010000000
VERSION     DAJTUN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.001"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            158..773
                     /locus_tag="OO470_000001"
                     /pseudo
     CDS             158..773
                     /locus_tag="OO470_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            876..1199
                     /locus_tag="OO470_000002"
     CDS             876..1199
                     /locus_tag="OO470_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6249401.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1266..2723)
                     /gene="nuoN"
                     /locus_tag="OO470_000003"
     CDS             complement(1266..2723)
                     /gene="nuoN"
                     /locus_tag="OO470_000003"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6249402.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2730..4259)
                     /gene="nuoM"
                     /locus_tag="OO470_000004"
     CDS             complement(2730..4259)
                     /gene="nuoM"
                     /locus_tag="OO470_000004"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6249403.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4423..6264)
                     /gene="nuoL"
                     /locus_tag="OO470_000005"
     CDS             complement(4423..6264)
                     /gene="nuoL"
                     /locus_tag="OO470_000005"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6249404.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6261..6563)
                     /gene="nuoK"
                     /locus_tag="OO470_000006"
     CDS             complement(6261..6563)
                     /gene="nuoK"
                     /locus_tag="OO470_000006"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6249405.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6560..7114)
                     /gene="nuoJ"
                     /locus_tag="OO470_000007"
     CDS             complement(6560..7114)
                     /gene="nuoJ"
                     /locus_tag="OO470_000007"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6249406.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7126..7668)
                     /gene="nuoI"
                     /locus_tag="OO470_000008"
     CDS             complement(7126..7668)
                     /gene="nuoI"
                     /locus_tag="OO470_000008"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6249407.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7683..8660)
                     /gene="nuoH"
                     /locus_tag="OO470_000009"
     CDS             complement(7683..8660)
                     /gene="nuoH"
                     /locus_tag="OO470_000009"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6249408.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8657..11383)
                     /gene="nuoG"
                     /locus_tag="OO470_000010"
     CDS             complement(8657..11383)
                     /gene="nuoG"
                     /locus_tag="OO470_000010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6249409.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11436..12773)
                     /gene="nuoF"
                     /locus_tag="OO470_000011"
     CDS             complement(11436..12773)
                     /gene="nuoF"
                     /locus_tag="OO470_000011"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6249410.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12770..13270)
                     /gene="nuoE"
                     /locus_tag="OO470_000012"
     CDS             complement(12770..13270)
                     /gene="nuoE"
                     /locus_tag="OO470_000012"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6249411.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13273..15075)
                     /gene="nuoC"
                     /locus_tag="OO470_000013"
     CDS             complement(13273..15075)
                     /gene="nuoC"
                     /locus_tag="OO470_000013"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6249412.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15169..15831)
                     /gene="nuoB"
                     /locus_tag="OO470_000014"
     CDS             complement(15169..15831)
                     /gene="nuoB"
                     /locus_tag="OO470_000014"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6249413.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15847..16290)
                     /gene="nuoA"
                     /locus_tag="OO470_000015"
     CDS             complement(15847..16290)
                     /gene="nuoA"
                     /locus_tag="OO470_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6249414.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16921..17859)
                     /gene="lrhA"
                     /locus_tag="OO470_000016"
     CDS             complement(16921..17859)
                     /gene="lrhA"
                     /locus_tag="OO470_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6249415.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18779..19996
                     /gene="alaA"
                     /locus_tag="OO470_000017"
     CDS             18779..19996
                     /gene="alaA"
                     /locus_tag="OO470_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6249416.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20080..20679
                     /gene="yfbR"
                     /locus_tag="OO470_000018"
     CDS             20080..20679
                     /gene="yfbR"
                     /locus_tag="OO470_000018"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6249417.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20738..22570)
                     /locus_tag="OO470_000019"
     CDS             complement(20738..22570)
                     /locus_tag="OO470_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6249418.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22657..23307)
                     /gene="hxpA"
                     /locus_tag="OO470_000020"
     CDS             complement(22657..23307)
                     /gene="hxpA"
                     /locus_tag="OO470_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6249419.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23318..23812)
                     /locus_tag="OO470_000021"
     CDS             complement(23318..23812)
                     /locus_tag="OO470_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6249420.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23895..24350)
                     /locus_tag="OO470_000022"
     CDS             complement(23895..24350)
                     /locus_tag="OO470_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6249421.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24688..25890
                     /gene="ackA"
                     /locus_tag="OO470_000023"
     CDS             24688..25890
                     /gene="ackA"
                     /locus_tag="OO470_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6249422.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25965..28109
                     /gene="pta"
                     /locus_tag="OO470_000024"
     CDS             25965..28109
                     /gene="pta"
                     /locus_tag="OO470_000024"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6249423.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28299..29819
                     /gene="yfcC"
                     /locus_tag="OO470_000025"
     CDS             28299..29819
                     /gene="yfcC"
                     /locus_tag="OO470_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6249424.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29852..30394)
                     /gene="yfcD"
                     /locus_tag="OO470_000026"
     CDS             complement(29852..30394)
                     /gene="yfcD"
                     /locus_tag="OO470_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6249425.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30452..31003)
                     /gene="yfcE"
                     /locus_tag="OO470_000027"
     CDS             complement(30452..31003)
                     /gene="yfcE"
                     /locus_tag="OO470_000027"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6249426.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31059..31703)
                     /gene="yfcF"
                     /locus_tag="OO470_000028"
     CDS             complement(31059..31703)
                     /gene="yfcF"
                     /locus_tag="OO470_000028"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6249427.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31839..32485
                     /gene="yfcG"
                     /locus_tag="OO470_000029"
                     /pseudo
     CDS             31839..32485
                     /gene="yfcG"
                     /locus_tag="OO470_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
     gene            32542..32904
                     /gene="folX"
                     /locus_tag="OO470_000030"
     CDS             32542..32904
                     /gene="folX"
                     /locus_tag="OO470_000030"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6249428.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32925..33818
                     /locus_tag="OO470_000031"
     CDS             32925..33818
                     /locus_tag="OO470_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6249429.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33866..34756)
                     /gene="rpnB"
                     /locus_tag="OO470_000032"
     CDS             complement(33866..34756)
                     /gene="rpnB"
                     /locus_tag="OO470_000032"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6249430.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34953..35726)
                     /gene="hisP"
                     /locus_tag="OO470_000033"
     CDS             complement(34953..35726)
                     /gene="hisP"
                     /locus_tag="OO470_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6249431.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35734..36450)
                     /locus_tag="OO470_000034"
     CDS             complement(35734..36450)
                     /locus_tag="OO470_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6249432.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36447..37133)
                     /gene="hisQ"
                     /locus_tag="OO470_000035"
     CDS             complement(36447..37133)
                     /gene="hisQ"
                     /locus_tag="OO470_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6249433.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37223..38005)
                     /gene="hisJ"
                     /locus_tag="OO470_000036"
     CDS             complement(37223..38005)
                     /gene="hisJ"
                     /locus_tag="OO470_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6249434.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWALKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38226..39008)
                     /gene="argT"
                     /locus_tag="OO470_000037"
     CDS             complement(38226..39008)
                     /gene="argT"
                     /locus_tag="OO470_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6249435.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39274..39843)
                     /gene="ubiX"
                     /locus_tag="OO470_000038"
     CDS             complement(39274..39843)
                     /gene="ubiX"
                     /locus_tag="OO470_000038"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6249436.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39938..41455)
                     /gene="purF"
                     /locus_tag="OO470_000039"
     CDS             complement(39938..41455)
                     /gene="purF"
                     /locus_tag="OO470_000039"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6249437.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41492..41980)
                     /gene="cvpA"
                     /locus_tag="OO470_000040"
     CDS             complement(41492..41980)
                     /gene="cvpA"
                     /locus_tag="OO470_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6249438.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42239..42901)
                     /gene="dedD"
                     /locus_tag="OO470_000041"
     CDS             complement(42239..42901)
                     /gene="dedD"
                     /locus_tag="OO470_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6249439.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42891..44159)
                     /gene="folC"
                     /locus_tag="OO470_000042"
     CDS             complement(42891..44159)
                     /gene="folC"
                     /locus_tag="OO470_000042"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6249440.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44229..45143)
                     /gene="accD"
                     /locus_tag="OO470_000043"
     CDS             complement(44229..45143)
                     /gene="accD"
                     /locus_tag="OO470_000043"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6249441.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45299..45958)
                     /locus_tag="OO470_000044"
     CDS             complement(45299..45958)
                     /locus_tag="OO470_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6249442.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46041..46853)
                     /gene="truA"
                     /locus_tag="OO470_000045"
     CDS             complement(46041..46853)
                     /gene="truA"
                     /locus_tag="OO470_000045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6249443.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46853..47866)
                     /locus_tag="OO470_000046"
     CDS             complement(46853..47866)
                     /locus_tag="OO470_000046"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6249444.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47932..49068)
                     /gene="pdxB"
                     /locus_tag="OO470_000047"
     CDS             complement(47932..49068)
                     /gene="pdxB"
                     /locus_tag="OO470_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6249445.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49167..50162
                     /gene="flk"
                     /locus_tag="OO470_000048"
     CDS             49167..50162
                     /gene="flk"
                     /locus_tag="OO470_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6249446.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50159..51337)
                     /locus_tag="OO470_000049"
     CDS             complement(50159..51337)
                     /locus_tag="OO470_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6249447.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51592..52833)
                     /gene="fabB"
                     /locus_tag="OO470_000050"
     CDS             complement(51592..52833)
                     /gene="fabB"
                     /locus_tag="OO470_000050"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6249448.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52992..54998
                     /gene="mnmC"
                     /locus_tag="OO470_000051"
     CDS             52992..54998
                     /gene="mnmC"
                     /locus_tag="OO470_000051"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6249449.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55119..55397)
                     /locus_tag="OO470_000052"
     CDS             complement(55119..55397)
                     /locus_tag="OO470_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6249450.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55431..55979)
                     /gene="epmC"
                     /locus_tag="OO470_000053"
     CDS             complement(55431..55979)
                     /gene="epmC"
                     /locus_tag="OO470_000053"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6249451.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55979..56788)
                     /locus_tag="OO470_000054"
     CDS             complement(55979..56788)
                     /locus_tag="OO470_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6249452.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56788..57612)
                     /gene="mepA"
                     /locus_tag="OO470_000055"
     CDS             complement(56788..57612)
                     /gene="mepA"
                     /locus_tag="OO470_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6249453.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57616..58701)
                     /gene="aroC"
                     /locus_tag="OO470_000056"
     CDS             complement(57616..58701)
                     /gene="aroC"
                     /locus_tag="OO470_000056"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6249454.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58736..59668)
                     /gene="prmB"
                     /locus_tag="OO470_000057"
     CDS             complement(58736..59668)
                     /gene="prmB"
                     /locus_tag="OO470_000057"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6249455.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59834..60385
                     /gene="smrB"
                     /locus_tag="OO470_000058"
     CDS             59834..60385
                     /gene="smrB"
                     /locus_tag="OO470_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6249456.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60489..61379)
                     /locus_tag="OO470_000059"
     CDS             complement(60489..61379)
                     /locus_tag="OO470_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6249457.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61366..61890)
                     /locus_tag="OO470_000060"
     CDS             complement(61366..61890)
                     /locus_tag="OO470_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6249458.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61887..62357)
                     /locus_tag="OO470_000061"
     CDS             complement(61887..62357)
                     /locus_tag="OO470_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6249459.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62354..62902)
                     /locus_tag="OO470_000062"
     CDS             complement(62354..62902)
                     /locus_tag="OO470_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6249460.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62907..63629)
                     /locus_tag="OO470_000063"
                     /pseudo
     CDS             complement(62907..63629)
                     /locus_tag="OO470_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63649..64371)
                     /locus_tag="OO470_000064"
     CDS             complement(63649..64371)
                     /locus_tag="OO470_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6249461.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64431..65663)
                     /locus_tag="OO470_000065"
     CDS             complement(64431..65663)
                     /locus_tag="OO470_000065"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6249462.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(65712..66290)
                     /locus_tag="OO470_000066"
                     /pseudo
     CDS             complement(65712..66290)
                     /locus_tag="OO470_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66372..66935)
                     /locus_tag="OO470_000067"
     CDS             complement(66372..66935)
                     /locus_tag="OO470_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6249463.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(67619..68104)
                     /gene="sixA"
                     /locus_tag="OO470_000068"
     CDS             complement(67619..68104)
                     /gene="sixA"
                     /locus_tag="OO470_000068"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6249464.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68307..70451)
                     /gene="fadJ"
                     /locus_tag="OO470_000069"
                     /pseudo
     CDS             complement(68307..70451)
                     /gene="fadJ"
                     /locus_tag="OO470_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70451..71761)
                     /gene="fadI"
                     /locus_tag="OO470_000070"
     CDS             complement(70451..71761)
                     /gene="fadI"
                     /locus_tag="OO470_000070"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6249465.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(71941..72225)
                     /locus_tag="OO470_000071"
     CDS             complement(71941..72225)
                     /locus_tag="OO470_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6249466.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72597..73937
                     /gene="fadL"
                     /locus_tag="OO470_000072"
     CDS             72597..73937
                     /gene="fadL"
                     /locus_tag="OO470_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6249467.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            74688..75362
                     /locus_tag="OO470_000073"
     CDS             74688..75362
                     /locus_tag="OO470_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249468.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(75544..76299)
                     /gene="mlaA"
                     /locus_tag="OO470_000074"
     CDS             complement(75544..76299)
                     /gene="mlaA"
                     /locus_tag="OO470_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6249469.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            76593..77525
                     /locus_tag="OO470_000075"
     CDS             76593..77525
                     /locus_tag="OO470_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6249470.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            77599..77673
                     /locus_tag="OO470_000076"
     tRNA            77599..77673
                     /locus_tag="OO470_000076"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77632..77634,aa:Arg,seq:cct)
     gene            77835..>77979
                     /locus_tag="OO470_000077"
     CDS             77835..>77979
                     /locus_tag="OO470_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6249471.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEK"
BASE COUNT        19706 a        20874 c        19161 g        18238 t
ORIGIN      
        1 aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata cctctaccaa
       61 gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac gccttttatt
      121 gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt tcactccctc
      181 tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac acgtaagtcc
      241 tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac gtaatgccgg
      301 caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg tcagtagcgg
      361 tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg cctggaagcc
      421 agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac tttattgctg
      481 tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca ttgttgtcat
      541 cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt attgattttt
      601 ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact gacaccgctt
      661 tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga
      721 aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa taataaaaat
      781 ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg tctgttttaa
      841 gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc tattttgcag
      901 aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata
      961 atatacatga cacgctttca ggattacgcc gactggaatc actggatcga agtgagctgc
     1021 gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt
     1081 cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa aagctgatta
     1141 gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat
     1201 ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta
     1261 acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg tggccataca
     1321 cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc
     1381 cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag gctcaccgcc
     1441 acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac
     1501 cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc
     1561 atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga
     1621 tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg
     1681 gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa
     1741 acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga
     1801 tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcaac
     1861 gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc
     1921 gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg
     1981 attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct
     2041 ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcatg
     2101 ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc
     2161 gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaaa
     2221 gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg
     2281 cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac
     2341 agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc aattaacacc
     2401 aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta
     2461 caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg
     2521 cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag cgaaaccagc
     2581 gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca
     2641 atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc
     2701 aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca
     2761 aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag
     2821 aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac
     2881 atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc
     2941 aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc
     3001 accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg
     3061 ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc
     3121 ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga
     3181 ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg
     3241 ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc catgtgggaa
     3301 accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat ccacgcaccg
     3361 tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc cgacgcgttc
     3421 gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag caagatcccc
     3481 gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc
     3541 agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat caacagatat
     3601 tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg
     3661 gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac cagaccactc
     3721 gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct
     3781 ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag
     3841 aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc
     3901 aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc
     3961 agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc aatggcgaga
     4021 tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga
     4081 attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc
     4141 agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg
     4201 gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg
     4261 gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac
     4321 agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag cttatccgac
     4381 ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa
     4441 cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc gcagataacc
     4501 gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg ggatgttcat
     4561 cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa
     4621 cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac tcagcaggcg
     4681 gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc acagccaggc
     4741 tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct
     4801 gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc
     4861 gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt
     4921 cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt
     4981 gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt
     5041 cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag
     5101 tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg
     5161 cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga
     5221 tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc
     5281 ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc
     5341 gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac
     5401 cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt
     5461 acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac
     5521 aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt
     5581 accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc
     5641 aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta
     5701 aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt
     5761 cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca
     5821 caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac
     5881 taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc
     5941 gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag
     6001 catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga
     6061 catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc
     6121 gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac
     6181 gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat
     6241 aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc
     6301 tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg
     6361 aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg
     6421 accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc
     6481 agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc
     6541 agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt
     6601 tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca
     6661 gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg
     6721 taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga
     6781 ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg ccaatccaca
     6841 cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca
     6901 tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac
     6961 cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc gaaataatca
     7021 ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg
     7081 ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct
     7141 cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc
     7201 catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag
     7261 atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt
     7321 taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga
     7381 gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag
     7441 cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc
     7501 gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg
     7561 ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat
     7621 actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc
     7681 ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc
     7741 ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg cggtaacgac
     7801 gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat
     7861 ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg
     7921 gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca
     7981 atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga
     8041 cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc
     8101 cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc
     8161 acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc
     8221 atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa
     8281 accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc
     8341 caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc
     8401 ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac
     8461 attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtcca
     8521 taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag
     8581 gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt
     8641 tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca
     8701 gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagtcctt
     8761 cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac
     8821 ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca
     8881 tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtagt
     8941 acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt
     9001 aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt
     9061 tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc
     9121 aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg
     9181 tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca
     9241 tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga
     9301 atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc gccacaacag
     9361 cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag gtgctgtgca
     9421 gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagccggat
     9481 cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta ccgtcgcttt
     9541 cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct gtgcgttgat
     9601 gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcagaag
     9661 cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg gtttccagtt
     9721 cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg
     9781 aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt
     9841 tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt
     9901 tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc tcaggttcga
     9961 taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg
    10021 cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgagtgtcat
    10081 caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa
    10141 tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct ttcaccgcct
    10201 gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta
    10261 ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca
    10321 ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt
    10381 cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa
    10441 taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg
    10501 cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca
    10561 ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac
    10621 cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac
    10681 agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt
    10741 aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac
    10801 cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg
    10861 cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt
    10921 agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt
    10981 gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct
    11041 gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta
    11101 accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg
    11161 tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt
    11221 tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc
    11281 agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc
    11341 cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt
    11401 tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc
    11461 ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat
    11521 tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca
    11581 cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc
    11641 ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg
    11701 cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc
    11761 agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctg
    11821 cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag
    11881 tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg cataccaccg
    11941 gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga
    12001 tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg
    12061 ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg
    12121 ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag
    12181 cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg
    12241 tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt
    12301 ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct
    12361 tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag
    12421 atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag
    12481 gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct
    12541 ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga
    12601 ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc
    12661 gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg
    12721 cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc
    12781 gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca
    12841 tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc
    12901 catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac
    12961 cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa
    13021 ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa
    13081 tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca cgctgcttct
    13141 gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt
    13201 gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat
    13261 tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac
    13321 aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata
    13381 actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta
    13441 actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg
    13501 cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc
    13561 tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt
    13621 gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg
    13681 ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc
    13741 agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc
    13801 agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc
    13861 tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca
    13921 gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg ctacgccgcc
    13981 aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat
    14041 tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa
    14101 ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat
    14161 gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg
    14221 catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg
    14281 ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac
    14341 gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga
    14401 cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat
    14461 cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt
    14521 cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc
    14581 tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc
    14641 aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt
    14701 cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc
    14761 gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt
    14821 gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt
    14881 ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc
    14941 ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat
    15001 taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt
    15061 catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa
    15121 tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc
    15181 aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg
    15241 catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt
    15301 gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca
    15361 gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat
    15421 atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag
    15481 catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac
    15541 caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac
    15601 gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt
    15661 atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa
    15721 cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg
    15781 gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc
    15841 tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg
    15901 tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag
    15961 taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga
    16021 ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac
    16081 cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc
    16141 aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag
    16201 catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc
    16261 gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc
    16321 actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt
    16381 acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat
    16441 aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata tcaacaccat
    16501 tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt
    16561 gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg
    16621 tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt tgttattcca
    16681 taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt
    16741 ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa ataaacagtt
    16801 ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa
    16861 aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga
    16921 ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct
    16981 gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt
    17041 attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc
    17101 tactccactc agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc
    17161 aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg
    17221 ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc
    17281 atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta
    17341 ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt
    17401 taccatcaaa tccacttcct gggattccag catttctgcc atataggcat tacgtttaac
    17461 gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa
    17521 gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa
    17581 cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag
    17641 ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc
    17701 aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc
    17761 ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc
    17821 gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc
    17881 tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc
    17941 tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa
    18001 aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg
    18061 gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata
    18121 aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg
    18181 tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat gcgagcattt
    18241 taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt
    18301 atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa
    18361 ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct
    18421 tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat
    18481 atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac
    18541 agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata acattgcatg
    18601 taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac aatcgagcct
    18661 tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg ggagtacatt
    18721 gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt caagattcat
    18781 gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc gtggtccggt
    18841 gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac tgaacatcgg
    18901 caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg tgatacgcaa
    18961 cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc gtaaagccat
    19021 catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata tttacatcgg
    19081 caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca gcggggacga
    19141 aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc tttccagcgg
    19201 taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc tcgatgatat
    19261 tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa ataacccaac
    19321 cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac gtcagcataa
    19381 tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg ctgagcatca
    19441 ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac tgtcgaaaac
    19501 gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga aaaaacacgc
    19561 caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg ctaacgttcc
    19621 tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg aatttattac
    19681 ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca acgatattcc
    19741 gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa tcgacgccaa
    19801 acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc aggaaaaagt
    19861 tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc gcattgtcac
    19921 gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt tcctttctgg
    19981 ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag tgttatcccg
    20041 ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag ccattttttt
    20101 gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa cgtgcggacg
    20161 gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct ggcagctatc
    20221 aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact ggcgatgtac
    20281 cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata cttcaatgcg
    20341 caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact ggtcgatatg
    20401 gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc atatagcgat
    20461 gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa atgtctggaa
    20521 gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga agcgacgctt
    20581 gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc cagcttccat
    20641 ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt ctgcgtcgca
    20701 tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac agcatcggga
    20761 tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac ttcacaaagt
    20821 cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa gacaccggtg
    20881 tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa ggcgacactc
    20941 ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc gcggtattag
    21001 agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc atatgtggcc
    21061 cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct cccgttttct
    21121 gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc cagtgaatgg
    21181 atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg caggcgatga
    21241 tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt agcaccagac
    21301 agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc tcttccggta
    21361 agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt ttccagttac
    21421 ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa ccttccagcg
    21481 ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga aaaccaattt
    21541 cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct gccatgccca
    21601 catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc agcaattgct
    21661 cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc aaaacgtcac
    21721 gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa cgccgccagc
    21781 gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca tccagccgtt
    21841 gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg gtcagtcgat
    21901 attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc ggggtctggg
    21961 tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc accagcacga
    22021 ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca cgcagcaatt
    22081 cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg ctaatcagcc
    22141 cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc atggcaacgc
    22201 ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac gcgccaagcc
    22261 ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc gccactttga
    22321 ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg ccaataataa
    22381 acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact tcggggaccg
    22441 tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca tccatacgca
    22501 ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc caaatcaatt
    22561 caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc cataaaaaag
    22621 ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa catcgccatt
    22681 tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga cctcattcag
    22741 gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg ccaggccaga
    22801 aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa gccccagcag
    22861 ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc gctcagcggt
    22921 tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg ctaccggcat
    22981 ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat tgagtaaggc
    23041 gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt gctccagacg
    23101 cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc gcaaagaggt
    23161 gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc catgacgtct
    23221 ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg ttccatcaag
    23281 atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata atttgattaa
    23341 tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta agcatgcgct
    23401 gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg tcaaaatggg
    23461 tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt gcttcagtgg
    23521 cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg tcctgcaaat
    23581 tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata gtgcggcagg
    23641 tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat ccgtaaccac
    23701 gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg agcatagtca
    23761 tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc attgttgact
    23821 cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct tttatatgcg
    23881 taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt gaaggcgcgc
    23941 ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc caacacctgt
    24001 ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc agggggtaat
    24061 ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact taaggcgaac
    24121 agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca gcagagagta
    24181 aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat aacgcgattt
    24241 tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta atgctgtccg
    24301 cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat gcttcacctc
    24361 aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg gacaacgttc
    24421 aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga aggcctaagt
    24481 agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg aaaattacgc
    24541 aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc agtgcatgat
    24601 gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg acgttttttt
    24661 agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt tctgaactgc
    24721 ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga gtacctttct
    24781 ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat ggacggcaat
    24841 aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa ctttatcgtt
    24901 aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat cggtcaccgt
    24961 atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc tgttattcag
    25021 ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct gatcggtatc
    25081 gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt atttgacacc
    25141 gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta caacctgtac
    25201 aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta tgtaacccag
    25261 gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac ctgccacctg
    25321 ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac ctctatgggt
    25381 ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga tccggcgatc
    25441 atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa actgctgacc
    25501 aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta tgttgaagac
    25561 aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca ccgcctggcg
    25621 aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt tgcattcact
    25681 ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa actgggcgtg
    25741 ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa atctggtttc
    25801 atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga actggttatc
    25861 gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc tggcggtgct
    25921 gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc cgtattatta
    25981 tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg
    26041 caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag ccgcgtaccg
    26101 gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc accacgacgg
    26161 ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat cagaaagatg
    26221 tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct gaagtcgttc
    26281 tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg aactacgaaa
    26341 tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact gacaccccgg
    26401 aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc aaaaacacca
    26461 acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag ggtcgtactc
    26521 gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac aatgttgatc
    26581 cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg tggagctttg
    26641 acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc atcatcaacg
    26701 aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc agcattccgc
    26761 acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac cgtcctgacg
    26821 tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc ctgctgctga
    26881 ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct ttcgctaccg
    26941 gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc ctgcagagct
    27001 tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag gaatacgttg
    27061 ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag cgcagccgtc
    27121 gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa gcgggcaaac
    27181 gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct atctgtgctg
    27241 aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac cgtgttgcag
    27301 cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa gtggttcgcg
    27361 aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc gaaaccgttg
    27421 cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa caggatgaag
    27481 ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt ccgccgctgc
    27541 agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc atgctgctgc
    27601 cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc gctgaacagc
    27661 tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg
    27721 ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta gaaaaagttc
    27781 gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac ggtccgctgc
    27841 agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac tctccggttg
    27901 caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc acctacaaag
    27961 cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt atgcgcaaac
    28021 cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc atcgcgctga
    28081 ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt tgcgctgcgg
    28141 atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt tgcattaatc
    28201 ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct cactttgcat
    28261 acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc actgaatcca
    28321 aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt gttgctattt
    28381 taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag gaagtgcagt
    28441 atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca tttcgcattc
    28501 tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg acgggcgatg
    28561 aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg aaatacggga
    28621 cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc attgtgatgc
    28681 gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc gggaatgaaa
    28741 ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta tttggtatgg
    28801 gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg ctgggctatg
    28861 acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt gccagttcgt
    28921 ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg gtgctttctg
    28981 gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg atctttacca
    29041 tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc
    29101 gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt ggtgactggc
    29161 tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg
    29221 cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg attggcatca
    29281 tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca tcctttaccg
    29341 aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa gggattttgc
    29401 tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc ctcaacagca
    29461 ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg ttgctcttcc
    29521 aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta actatgccgt
    29581 tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg ctggcgttcc
    29641 agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta atggcaacgc
    29701 tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc ctgcttggac
    29761 tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc taccactaaa
    29821 aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc tctacggctt
    29881 catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct ggagtgaact
    29941 catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg acttcatctt
    30001 cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag acacggcaat
    30061 ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca atgcccaact
    30121 cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga actacaccgc
    30181 ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga cgctggacca
    30241 gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc agacactgtg
    30301 cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg acaatatcca
    30361 cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct ttttctggcg
    30421 ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc tgcgcaatga
    30481 tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg ccataacccg
    30541 ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa atctcccccc
    30601 gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc tggtttaaag
    30661 caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt tgtttttcca
    30721 gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga tccacttcgc
    30781 tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc cgttcagcga
    30841 ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc aacacgtcgc
    30901 caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca cgttccgtcg
    30961 ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac tcctcgtcga
    31021 aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg cttcgccgaa
    31081 agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata atccaccagg
    31141 cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc cagatcagta
    31201 tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa atgttctgcc
    31261 atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc ttttttcgcc
    31321 cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc gctgcgcagc
    31381 caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata aatacgttcc
    31441 caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga ttcactcaac
    31501 tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc ttgccacgtc
    31561 ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga caggcctttt
    31621 tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc atctgaccaa
    31681 agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg ctatcaaact
    31741 atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg cgctttctat
    31801 acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc tatttcgccc
    31861 cgacacccaa tggtcacaaa attacgctgt ttctcgaaga gcagagcttg attatcgctt
    31921 gattaaggta gacctgggga aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc
    31981 caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag
    32041 cctctttgag tctggtgcca ttttgttgta tctggctgag aaaacaggac tctttttgag
    32101 tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact
    32161 ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta
    32221 cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagcggct
    32281 ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc
    32341 gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg cagtcaagaa
    32401 ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact
    32461 cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag
    32521 taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt
    32581 cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt
    32641 atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat
    32701 gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc
    32761 tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg
    32821 acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc
    32881 atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac atagtgatca
    32941 ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc
    33001 aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga
    33061 cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc
    33121 tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag cgtctctgcc
    33181 aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc gacacgccgc
    33241 cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg
    33301 ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag
    33361 agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg
    33421 tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg
    33481 gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca
    33541 acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct
    33601 acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta
    33661 ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca gtactggtat
    33721 taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt
    33781 acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg
    33841 ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa
    33901 gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa
    33961 tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa
    34021 tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac
    34081 ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt
    34141 cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc
    34201 gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct
    34261 cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg
    34321 ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc
    34381 aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat
    34441 ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct
    34501 tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc
    34561 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttctagtt
    34621 taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata ttaataaaat
    34681 tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
    34741 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
    34801 acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt
    34861 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa
    34921 tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt
    34981 tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt
    35041 ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga
    35101 gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg taacacttcg
    35161 cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttctggttcc
    35221 atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga
    35281 tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac cgcccgctcg
    35341 cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccaac
    35401 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
    35461 aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg
    35521 atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa gttaatgcag
    35581 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca
    35641 ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg
    35701 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
    35761 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
    35821 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc
    35881 gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag
    35941 catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat
    36001 aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc
    36061 atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag
    36121 cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt
    36181 tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact gaacatacag
    36241 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
    36301 ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat
    36361 aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg
    36421 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag
    36481 cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg
    36541 ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct
    36601 ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc
    36661 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga
    36721 aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc tttcggcact
    36781 gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg
    36841 ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc
    36901 atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa gtccggcacg
    36961 cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
    37021 ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc
    37081 tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct
    37141 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
    37201 ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg
    37261 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc
    37321 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
    37381 ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt
    37441 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
    37501 aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttagtgcc cagtgttcat
    37561 taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact
    37621 cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca
    37681 gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
    37741 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg ttttcgacaa
    37801 acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata tcgaaaccaa
    37861 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc
    37921 ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc agaaccagag
    37981 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
    38041 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
    38101 cccgttagac gtattatttt tcattaaaaa tgcttaagta tgtatttcaa tgcaccacga
    38161 tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg
    38221 acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg
    38281 ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct
    38341 ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca
    38401 gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca
    38461 tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg
    38521 gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta agcttcctgg
    38581 gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc
    38641 ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca
    38701 atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc gatttttttc
    38761 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc
    38821 atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca
    38881 tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc
    38941 gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc gagaatcgac
    39001 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
    39061 tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt
    39121 taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca ggtatagcgg
    39181 ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt
    39241 gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag
    39301 atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac
    39361 atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc
    39421 gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac
    39481 gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
    39541 atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat
    39601 ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgagcgt
    39661 gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
    39721 gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
    39781 taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt
    39841 cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc
    39901 aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga
    39961 gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga
    40021 ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc
    40081 attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat
    40141 cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt
    40201 gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt
    40261 ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
    40321 tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc
    40381 ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc agctgctggc
    40441 ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt
    40501 tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg atcgggatca
    40561 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca
    40621 tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt
    40681 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
    40741 actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa
    40801 agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt
    40861 caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca
    40921 taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtgg
    40981 cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct
    41041 cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
    41101 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
    41161 gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
    41221 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
    41281 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
    41341 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
    41401 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
    41461 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
    41521 tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc agctgtgatt
    41581 tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg
    41641 caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg
    41701 acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca
    41761 gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc
    41821 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
    41881 atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc agagaggaaa
    41941 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
    42001 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
    42061 ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat
    42121 gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg
    42181 gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt
    42241 aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca
    42301 gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac
    42361 cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga
    42421 caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac
    42481 ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaggtggtg
    42541 cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag
    42601 gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg
    42661 cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct
    42721 gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc accagcggga
    42781 tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa
    42841 tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca
    42901 ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac
    42961 accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg
    43021 ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc
    43081 ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt
    43141 ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt
    43201 agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa
    43261 ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc
    43321 ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgcc
    43381 gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca
    43441 tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt
    43501 aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg
    43561 acaattgccg gtttttcgct gcggaagatg cctgctttct cgcgaccaat actttcgcga
    43621 tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca
    43681 tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca
    43741 agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg
    43801 gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc
    43861 aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg cgaactgtag
    43921 acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct
    43981 ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac gccaagacgc
    44041 gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc
    44101 agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg
    44161 gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt
    44221 atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc
    44281 ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc
    44341 aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca
    44401 ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg
    44461 gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat ggcgaagctt
    44521 gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc
    44581 tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc
    44641 tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttcctgcgcc
    44701 tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag
    44761 gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc
    44821 agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt atacttcttg
    44881 gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc cacaaggctt
    44941 ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac
    45001 ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta
    45061 tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt
    45121 ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt cgatgccagt
    45181 tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat
    45241 taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt
    45301 attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa
    45361 tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc atcgggattg
    45421 taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt
    45481 tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg
    45541 ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt ttctcataaa
    45601 actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga ttactgaata
    45661 acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca atcgcggcga
    45721 tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt gatgccaacg
    45781 ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc agaccggttt
    45841 cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc gcgactagct
    45901 ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat
    45961 ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa
    46021 tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga
    46081 agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt
    46141 gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg
    46201 ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag
    46261 gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt aatatgcata
    46321 acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc
    46381 tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa
    46441 tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga
    46501 taacggcgag ccgtggcgct aaatcgtgca tgaaaatcat caggtacagc tttaacccaa
    46561 cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc cgcgtctttg
    46621 cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc tgcgtcagta
    46681 cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc
    46741 ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg
    46801 tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca
    46861 gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa
    46921 cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc
    46981 agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg aattcgttct
    47041 cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca
    47101 gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga
    47161 cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga
    47221 tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg
    47281 ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac
    47341 tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg
    47401 taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct
    47461 ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa
    47521 acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc
    47581 tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt
    47641 tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca
    47701 cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg
    47761 cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca
    47821 tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca
    47881 gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc
    47941 cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc
    48001 acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc
    48061 gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata
    48121 caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg
    48181 gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat gcccaataaa
    48241 cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca gggtatagcc
    48301 tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa gttccggctc
    48361 gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc aggtcagcag
    48421 cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc cgggcttcag
    48481 gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat ctttaaagag
    48541 tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg agcggaaatc
    48601 accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc
    48661 ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg
    48721 gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga aaacatattc
    48781 caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa
    48841 ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt taatgggttt
    48901 tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc
    48961 tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac ccaaacggct
    49021 aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc
    49081 tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag gcatacttgc
    49141 gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct cctcctgggc
    49201 agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc
    49261 agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacaacaaa
    49321 gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc
    49381 tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa
    49441 aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaatc
    49501 atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc
    49561 tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc
    49621 cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc
    49681 taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc
    49741 aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta
    49801 accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc
    49861 tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat
    49921 cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg
    49981 ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata
    50041 tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc
    50101 gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt
    50161 aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg
    50221 caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga
    50281 cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg
    50341 aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc
    50401 cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca
    50461 agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg
    50521 gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa
    50581 agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg
    50641 caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc
    50701 tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg
    50761 cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc
    50821 tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt
    50881 tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc caggtcagag
    50941 cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg accaacaggg
    51001 caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca
    51061 gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga tcggccagtc
    51121 gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatgccga
    51181 ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca
    51241 accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc gaaaaactgg
    51301 cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca
    51361 aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg ttggcaggat
    51421 ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tagaactggt
    51481 ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga
    51541 ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccgg
    51601 cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa
    51661 gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt
    51721 cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga taaagccgtg
    51781 ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca gtgagtgacc
    51841 ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt cacggatagc
    51901 tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca ggtaatcgat
    51961 tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt cgccagacgg
    52021 agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag catagatgta
    52081 agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc cgccgccagc
    52141 gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg gggtgtcgtt
    52201 gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc agcacagctc
    52261 ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct ctactgcgtt
    52321 accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa cgccatgaat
    52381 tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag tgaccacata
    52441 cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga acacctggaa
    52501 acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat tctggtaagc
    52561 ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg cataaatgga
    52621 tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat ccagttttat
    52681 gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga aagtgatccc
    52741 tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga tgctggaaac
    52801 aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt cgcacataga
    52861 gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga tcagccattt
    52921 aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg cctgtaagac
    52981 gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt aatgctgagg
    53041 gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac gggctggaag
    53101 agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct gagcatccac
    53161 atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc ctgacgctat
    53221 ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt
    53281 tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg catcaacact
    53341 ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg cccttgcccg
    53401 gttgccatcg tttattgctc gatgaaggcc gcgtgacgct ggatttatgg tttggcgata
    53461 ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat gcctggtttc
    53521 tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg tttaacgcca
    53581 tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc
    53641 gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt gggcgcaaac
    53701 gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgctcc acgccgtggt
    53761 ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt attgccagcg
    53821 cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat tgcgcggatg
    53881 aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg ttattaagca
    53941 aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct cgtcggtttt
    54001 acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag ttaggctggg
    54061 atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc gcagaactgg
    54121 ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca aattgcagcg
    54181 gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt aatgtgctgg
    54241 aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag aatttatccc
    54301 gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca cacagcgtag
    54361 tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact ctcccggtgt
    54421 attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca gagctgaagc
    54481 aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa catcattgta
    54541 ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac gatcagcagc
    54601 agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa gaggttgatg
    54661 tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg
    54721 taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg gcggagcaaa
    54781 aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg gctttaggtt
    54841 ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg
    54901 aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg ttatgggtgc
    54961 ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca gtgtttgccg
    55021 gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat aagacgcgat
    55081 aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga cgcctgctga
    55141 aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa ttcaccggca
    55201 ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca
    55261 tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata cccactggca
    55321 aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag aatgcgtgat
    55381 tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctgact tcagttgagc
    55441 gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata attttgtagt
    55501 gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc catcacctgc
    55561 gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc cagattgtcg
    55621 caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa cgcctgcggc
    55681 ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc cgggcaatac
    55741 cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca gtgcgaaatc
    55801 tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata cggtacttcc
    55861 gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt attaaattca
    55921 tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt actgttcatt
    55981 agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt aatttggcac
    56041 tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct ttgctcaaca
    56101 ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca aagcccgtcg
    56161 cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt gacgttgcgt
    56221 tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc ataacgaagg
    56281 ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa ccgacgcagc
    56341 caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct tcttcaccca
    56401 gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc aaaatctgcc
    56461 gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag ccgacaaagg
    56521 tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga ataaagtaga
    56581 tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc gcgttagcgg
    56641 gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg
    56701 agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac agcggagaaa
    56761 ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta gcgcctggca
    56821 ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg gttttggagg
    56881 ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta aaggttgatc
    56941 ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg cgcgatgctg
    57001 gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa ggcaaagttg
    57061 ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg cggcgagttt
    57121 gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac cgtcgcggga
    57181 aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag ttttcggcag
    57241 ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc cgttgaaacg
    57301 cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca gattgctcac
    57361 ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat agcgacgctg
    57421 atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag cgccgacaat
    57481 acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt gggttatttt
    57541 ttgccacggc gtcgctgcca ggctagcgct actggcaagc agagccagca gcgcaatcgc
    57601 ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat tttgcgcccg
    57661 ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga tcggcactgc
    57721 acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt cttcgccaaa
    57781 gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg ccatatgagc
    57841 aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt tgctctggaa
    57901 accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta cgtcaaaacc
    57961 gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg cgatgtcggc
    58021 atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg caacaacggt
    58081 gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt catctaacgc
    58141 gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg accagtcttt
    58201 gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa taccaaattt
    58261 ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg tttcgcgggc
    58321 ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc
    58381 agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac gctggtcggt
    58441 attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa caccggagag
    58501 tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc cagggcgacg
    58561 acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc
    58621 gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc gaaagagttg
    58681 tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg tttacttaat
    58741 ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg agcataaaca
    58801 caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga tattgttcca
    58861 taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca gcaaggtaat
    58921 ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc
    58981 ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc gcgtcgacat
    59041 acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg aacagatcgg
    59101 aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt tcggcaaccg
    59161 ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag gcataagcac
    59221 aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc ggttgcttgc
    59281 tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc ggcaccagca
    59341 cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc aggtaagcca
    59401 ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt ttttcgctgg
    59461 aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag agcgatggta
    59521 acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac cagatatttg
    59581 ccgcgctgaa gcggcttacc gaccagcgca acatgtcctg aatggtttgc agctcattta
    59641 ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg caataatttc
    59701 ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt gagtgaggaa
    59761 aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga aaattagaaa
    59821 tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg ctctgtttcg
    59881 ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc gaccgcagcg
    59941 taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg atgccagcca
    60001 ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga aatatgttcg
    60061 cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt caccggaatt
    60121 gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg gcgcgttgat
    60181 tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc acgggaagca
    60241 tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga tggcatttca
    60301 ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg aagtggaaga
    60361 atggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag cgtaaacgcc
    60421 ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg taggcctgat
    60481 aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg aaaacgacac
    60541 gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc gtgtagtact
    60601 ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc acaaccgtaa
    60661 gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc atcattatca
    60721 gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga aagcgtggtg
    60781 gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac ataattttca
    60841 ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt atgcactgta
    60901 ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga tgcgatccaa
    60961 gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc acaacgaaaa
    61021 attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc agtcagtacc
    61081 aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc gcctttatac
    61141 gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt aacattgaaa
    61201 acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct cacactgtca
    61261 aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac aacactcaga
    61321 acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga aatactcaaa
    61381 ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc tctttgcctg
    61441 taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg tgacgcctgt
    61501 tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc cagcgccgga
    61561 tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt ctgttcctgt
    61621 caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt gaaacaccaa
    61681 tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg caggaaaatg
    61741 tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga ttaaattgcc
    61801 ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca tcaggctact
    61861 aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt ggcccaggcc
    61921 tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc cgttaacacc
    61981 ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg ccctgcctgt
    62041 ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc aaaatcgctt
    62101 ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc cgtatcgcag
    62161 gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc aataagcaca
    62221 tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg cggctgcact
    62281 aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca cagcaaaata
    62341 agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta aattcagctg
    62401 ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga actcccgagg
    62461 aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga tcactacttt
    62521 tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca ccgttaatca
    62581 cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg ttacaaacaa
    62641 gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg aatacattac
    62701 caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca agcacccgta
    62761 aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca caacatatca
    62821 gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc ccggcctcag
    62881 cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt gaaaaagagc
    62941 ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc catcttcaca
    63001 ttcagcggtg cagaggtttt cggcgacatc actattagcg agaaaccggc tgccggattg
    63061 ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg gttattgacg
    63121 gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt tttcatgctg
    63181 acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag tgctatttgc
    63241 agcgtgttgg ctttgtttga ttttggcggg atctcgcgca cgttgaagta gaacaggctt
    63301 tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac ctgcccgttc
    63361 atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact gatcttgtta
    63421 cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc gttattgcgc
    63481 aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg atctggcgtt
    63541 acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt cgtagcaccc
    63601 aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt attggttagc
    63661 tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct gaatgacagg
    63721 ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg gcagtaacaa
    63781 gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat gcgcaacacc
    63841 actccagaac acactcatgt gttcgccagg tttcacccca gccagataaa cattgccgtc
    63901 atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc caaacggagg
    63961 atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga tgacggcaaa
    64021 tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact gggttgcttc
    64081 ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat agttattgac
    64141 gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt taccttctac
    64201 cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca tattctgggt
    64261 acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg acaagcccgc
    64321 agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca gcgaaccatc
    64381 atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt tcaactgata
    64441 gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg tcccactgcc
    64501 atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag cgaaatgtac
    64561 atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga gatgctgaca
    64621 ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt tgtctgctcc
    64681 tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc cgcatcgcgg
    64741 aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg taccattccg
    64801 ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga gaagcgatat
    64861 ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact cacacggaag
    64921 gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt ggtgtgcgag
    64981 tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac accaagcgcc
    65041 gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc gttagcgatc
    65101 ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc ccactcttgc
    65161 ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta tggcatcgag
    65221 gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg aatatgcaac
    65281 gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg aacctgcgtt
    65341 tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg tgccacgccg
    65401 gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc agtactgaca
    65461 ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc ctcgcccagc
    65521 gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact ccactcccat
    65581 tttttttgag tttcatcgcc gctgaattca tcgtcatcat tactgcgagt atgttgatag
    65641 ttagtctgcc agtcagcacg catacgccac ggccctaggt taaccccaac cgtcccgttg
    65701 ccgctgatct cgttactatc atcaccgcca ttttcttcgt gccgggtttg tgcgttgatg
    65761 ctgtagtccg cgacgatccc ggagatgccg tcatcccaac gtgaaggcgg atcccagtcg
    65821 ggataagtat attcgaggta agcctgtggc agtgaaatga ctaatgcgga ctggcttaaa
    65881 tcagccttaa tttccatgcc ttccagttga ccggatttca ggcatttagc atcgtggctc
    65941 cattgcagat ttttcgccac gtcttctttt aaaccaaact gcgctaccag ttccggtgtc
    66001 agacaagcat agcttttgct cgcgtcatct tcaccagcat accagtaaat atcgtactct
    66061 tccgccaatg gctgtttatt tagttgaacc tgtaaattgt attttccggg ctcaacatat
    66121 ccctagctgg aaaaacgctt cagatcaatt tttgtgtcgc cttttaattc cagaaaacgg
    66181 gaatcaaact gaatgtcgtc ttcagcccag acactgctat aactccctga cattgccagc
    66241 gcaatgcacc acggaagaat ccgtaatcga aaaagagaat ggtcaggcat acacgtttct
    66301 atccgttttt attcttgttc ggtcaaaata catttaaatc ccccatagca gtgtcgctat
    66361 gggggtatgc cttacaggta agtaatctgg aaggtggtat ttgcttcaaa attacccaga
    66421 tctggcgcat cagctgcgcc gaccagccag gcgttaaagt tcagtgtctg cttcgctttg
    66481 cctttgttgg tagaagtgtc tttcactatt ttctggtcaa tacccatgcc gcttttgtaa
    66541 gaggtaccca gagagtcacc gatcgccagg ctaacattgt taaatgccgc accggtatcg
    66601 gtattgaaaa tggtgtaata attgccgcta tttgcagaag aaacggtacc agtaaaggta
    66661 gtggtcattg tttcctgagt atcaaatacg caatcttgca gacgaatctg gaaacttttc
    66721 ggcgtggtgg tgccgttatt tttcagtttt tcggcaccga tatcacccat atcaacttcc
    66781 agtttgtgat cgtctggaac gatagaacac actgagtctt caatggtgcc gtaaaaacgt
    66841 gcagtaccgt tgttacccgc agcgattgtt gccgtagagg tgaacacgcc catcaccatt
    66901 gccgcagcaa tagctgtttt aacaaactta ctcattattt gtccttaaac tataaataat
    66961 taaaaatata aacattgcaa tacattgatt cagtcaatag ccaatgttta tacccagcca
    67021 ttgaatacgg catgcgaccc aaaaagaaca ggtcgtagaa aattgtattc attggtgtgg
    67081 ggccgcatag tgatgtttcc acacccctga tccaatttaa ttttactaaa gagtaaatgt
    67141 agatttaatt aatatattga cagaagaaag gttatcttaa ggacatcctt aaaaaatacg
    67201 gaataagtat ttagaaaaaa tagaaagtat atcaataatg aatatcaaca gcttaaaaaa
    67261 caaacaatga acatataatg cgattggcat ttatccactt ttgacaatat aatatataaa
    67321 cactatagcc agaatttaat caagtttcaa ttacatcctg cctgattatt caactctatt
    67381 ttctctatta tctgtattaa taaatgacaa tgagtgtcaa acagatatta ttcatttcaa
    67441 catgtaactt tgggaaaaat caagatacgc gcagaagaat aatttccgaa tgaatgtaga
    67501 aagtgtttta tttatagata aggagagtct tcgatagtag tgaggttgac agatatccgc
    67561 tgcctaatga gcaattatgc ccaatgaatc tacctcattg ggcataattt ggaacacatc
    67621 agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat gtaccgtttc
    67681 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
    67741 ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc accagtaccg
    67801 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
    67861 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tcccctactt
    67921 cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
    67981 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta gtggtcagag
    68041 gacgaaccga atcactggcg gaatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
    68101 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
    68161 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
    68221 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc cgccattcag
    68281 cgcggattca tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa
    68341 ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac
    68401 tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
    68461 cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg
    68521 tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt cagcatcaac
    68581 atcacacacc gttcagcaac ctgcggtgcg gagagtcacc cctgcccttg tgcgccaatg
    68641 agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga
    68701 tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
    68761 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
    68821 ccggtgtcga ttcctacctc atccaaaagt tggattgggc ctaccggaaa accaaatttc
    68881 actagcgcgg catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg gatagcttca
    68941 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
    69001 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg cgccgatgta
    69061 cccgcatgag gaataatctc taccagcggc attttttcca ccggactgaa gaaatgcagg
    69121 ccaataactt gctcaggtcg cgcggcgtga gcggcgatat caccaatcgg taaagatgac
    69181 gtgttcgaag caaaaatggt atgagcggcg caattttgct ccacttccgc caccatctgt
    69241 tgttttaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
    69301 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
    69361 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
    69421 ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc agtgacataa
    69481 gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa tgccgcaggc
    69541 ggtgcatcac taccgggatc tttcttcacg tccgtactgg caaaaaagat actacgcaaa
    69601 gcttgcgatt gcggcgtcat cgccagttcg ccaaaggcgc gagcttcagc gtcatagccg
    69661 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
    69721 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
    69781 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
    69841 ttcttcgcca gctcaacagc ggcttccagc aaaatggagt gcggaacaac gtcatccacc
    69901 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgagaat catctctaat
    69961 gctgtactga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
    70021 ccaagttgta cttcagacaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
    70081 tggcacgcca gcgccagctc cagcccaccg cccaggcaag cgccatgaat agccgcgata
    70141 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagtgct
    70201 tccgcttctt gcgccgtttt gcagttgcca atcatgttga tgtctgctcc ggcgataaaa
    70261 ttgtccggtt tcgcggagat aaacaccacg ccgcgcaact ctttgttttc acggagttgc
    70321 ttaataatgg cgcgcacctg cgaggcaaat tccgccttca gggtattcat tttctcatcc
    70381 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat
    70441 gccatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
    70501 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
    70561 tccgcgcgcc ggtcgccgcg aagggatgtc cgtaagcaat cgaaccgcca agcacgttaa
    70621 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca cgagcaaaac
    70681 gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat
    70741 cgatcaatgt cagttcgctc atcgtcaaac cggcacgttc cagcgccagc ggtgttgacc
    70801 aggctggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg tagctgcgca
    70861 gataccccag cggcaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
    70921 ctgccgcgcc atcggtcagc ggcgtactgt ttgccgccgt taccgttccg tgtttgcgat
    70981 caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga atattgttgt
    71041 cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
    71101 ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
    71161 gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc atccgcaagc
    71221 cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa cgcaggcgag
    71281 agaagagttt caggcgctgg ctcatggttc gagctttgtt gacatcaacc agcacgtgcg
    71341 ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca ccggcaatcc
    71401 ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgcc tggaaactgg
    71461 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg gtaccgagaa
    71521 caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca aagaccagct
    71581 gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
    71641 ctaaatcaac cgtgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
    71701 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagtggtaaa acctgaccca
    71761 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt
    71821 tttttacatt tagccaagcg gagaaaaagg aaagtgggag ctatgacaca gtgaagacag
    71881 aagaaagaaa agaaaacgcc cctgccagca actgacaggg gcgtacgtac ccgagaggaa
    71941 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
    72001 atccaggcgc acaccgtcag actcttcagt gaaagtcggg gagattttgc acggttcgga
    72061 ttccacgcta cgggattttt cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
    72121 tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg tgccaacatc
    72181 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
    72241 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
    72301 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
    72361 aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca gctggatcgc
    72421 gtttcttaga tcatatttga aaaaagatgg aaacatactt gcaacattct agctggtccg
    72481 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
    72541 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
    72601 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
    72661 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
    72721 attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac cccgcattga
    72781 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtga
    72841 atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
    72901 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
    72961 ctattacctc taactatggt ctggcaacag agtttaatga tacttatgca ggcggctctg
    73021 tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
    73081 ataatgcatg gagctttggt cttggtttca acgccatcta cgctcgcgcg aaaattgaac
    73141 gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct cctgccgggc
    73201 aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
    73261 tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc ctgtatgaac
    73321 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
    73381 gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca attcctaccg
    73441 cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
    73501 tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg gcttacacca
    73561 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
    73621 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
    73681 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gctcagaatc
    73741 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcat
    73801 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
    73861 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
    73921 ttaactacgc gttctgataa cgcgttcgcc tagataaagt cacctgctat gcaggtgact
    73981 ttaactcccc cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa
    74041 cagcctgatg gcagcttgcc ctttcaaatc agtaagtagt atccacagac aaacctgaag
    74101 ggaaatgcat attttcaagc gttctgctcg tccttctcaa agagtttact tttctgcatt
    74161 tccaggatac tcaacccccc tggctattgt gcgcttatac actcaaatta aagataggtt
    74221 ctaaataaat gagcgttttt tgatagtcta tttcattagg taatatatat ttgtaacaaa
    74281 tcaatcaaca tggaataaaa tcatgctacc atctatttca atcaacaata ccagcgcagc
    74341 ttacccagaa tccatcaatg aaaataacaa tgatgaaatt aatggattag tacaagagtt
    74401 caaaaaccat ttttaatggt aaggaaggaa taagcacctg tattaaacat ctacttgagc
    74461 ttataaaaaa cgccatacga gtaaacgacg atccttatag atctaatatt aataattcct
    74521 cagttactta tattgatatt ggctccaatg atacagacca tattactatt ggtatcgaca
    74581 accaagaacc aatagaatta cctgcgaact ataaagacaa agaactcgtc cgtactatca
    74641 ttaatgacaa catagttgag aagactcatg atatcaataa caaggaaatg atcttcagcg
    74701 cattaaaaga aatatatgat ggagatcctg gttttatctt cgataagata tcacacaaac
    74761 tcagacatac ggtaacggaa tttgatgaga gcgggaaaag cgaaccaacg gacttattta
    74821 cctggtacgg taaagataaa aaaggcgact ctctcgctat tgtaattaaa aataaaaacg
    74881 gaaatgatta cttatttctc ggttactacg atcaggacga ctaccacatt caaagaggaa
    74941 ttcgtattaa tggtgatagt ctcacccaat attgtagtga aaacgccagg agtgcttcag
    75001 cgtggtttga aagcagtaaa gctatcatgg cagaatcatt tgcaactggt tccgatcatc
    75061 aggttgtaaa cgagctcaac ggggaaagac tgagagaacc taacgaagtt tttaaacgtt
    75121 taggtcgagc aataagatat aattttcaag tggacgatgc aaaatttaga cgcgataatg
    75181 taaaagaaat aatttctact ttattcgcta acaaagttga cgttgaccat cctgaaaata
    75241 aatacaaaga ctttaagaat ctcgaagata aagttgaaaa aagacttcaa aatcgccaga
    75301 cgaaatatca aaatgaaatt aatcaactat ctgcactagg tgttaatttt gatgatattt
    75361 aaaattattt ttccagtttt ttgatttttc aacgcctgaa tatcggaaat agtcagtgac
    75421 aaacatcagt atttttacat cattacgtcc gggtcattgt cggtttatct ccttttactt
    75481 gtggtaattg agggataaca acaaaaaagg tgagttttgc gactcacctt ttttatttgt
    75541 ttcttattca gaatcgatat cttttaaatc atcctgaatc gcttgcgcgt tcggattttc
    75601 ctgcggtttg agttcgccgc cattagcgat gaaatcatga cgctggaagt acgcttcgcg
    75661 caccataata taaggatcgg acgactgacg cagcagacca tcggaatcca gcagctgagc
    75721 gcgggtttcg atcccttcaa gcgtccattt acccacagac atcggccagg tcagccagga
    75781 aagaaccggg taaagagcat ccgccatatc accaccgtca tcacgcagcg tgaagctacc
    75841 gtagaacggt aactgaacgt aaggcccata acccacgcca taatgaccaa gcgtactacc
    75901 gaagcggtga ggttcagtcc gttgcagttt cgggttcgcc atccctgcaa catcaataaa
    75961 gccgcccatc cccaaaatgg tgttcaggaa aaagcgggta aagtggacca tcccctgata
    76021 agggtcgccc tgcaagaagt agttaaccat caccgcaggt tcttcaaggt tgccagtaaa
    76081 gttgctcaaa ccattacgcg ccggttgcgg aacataatca cgccaggcga cagcgaccgg
    76141 tcgaacaata tacgggtcta atacgttgaa gttgaagttg tacatggtgc ggttgaaccc
    76201 ttctaacggg tcagaacgcc cttgctgatc tgtaccggaa ctcgcacacc ccaccagaag
    76261 cgtagttccc agagcaagcg ccgacaggcg aagcttcata aatgtctccc tgttttttta
    76321 tggcttatgc agtttgccat ccatgacgga acgataccgt atccgcctgt ttaggtgtgg
    76381 gcgattgtaa cagcacgtca actgatgtcc agacgccctg atttgctgat ttgatcatag
    76441 cctggtaatc gccgccctgt aggctacttg attctataga aacagaaaaa ggcaaacgca
    76501 gccttttcta cattttcaga gtaactcccg ccagttgcga gcaaaaaagc cgctacgctt
    76561 tagctatacg tgctaattca agagaagaga ccatggacaa cgacaaaatt gatcaacaca
    76621 gcgacgaaat tgaagttgag agcgaagaaa aagagcgcgg caaaaaaata gaaatagatg
    76681 aagaccgact cccctcccga gcgatggcaa ttcatgagca tatccgccag gatggtgaaa
    76741 aagagctaga acgcgacgca atggcgctac tgtggtcagc cattgcggcg ggtctgtcga
    76801 tgggcgcttc gttactggca aaagggatat ttcatgccga actggaaggt gtgccgggca
    76861 gcttcttgct ggagaatctc ggttatacct ttggttttat tatcgtcata atggcccgcc
    76921 agcaattatt taccgaaaac accgtgactg cggtactacc cgtcatgcaa aaaccgacaa
    76981 tgagcaacgt tggcttactt atgcggttat ggggagtcgt gctgctgggt aatattctcg
    77041 ggactggtat tgctgcgtgg gcatttgaat atatgcctat cttcaatgaa gaaactcgcg
    77101 atgcatttgt caaaatcggc atggatgtga tgaagaacac ccccagcgag atgtttgcca
    77161 acgcgatcat ttccggctgg ctgatcgcca ctatggtatg gatgtttcct gcagcaggtg
    77221 cggcaaagat tgtggtgatt atattgatga cctggcttat tgccctgggt gacaccaccc
    77281 atatcgtggt cggttctgtt gaaatcctct atctggtgtt taacggtacg ctgcactgga
    77341 gcgatttcat ctggcccttc gcactaccta ctttagcggg gaacatctgc ggcggcacct
    77401 ttatcttcgc gttaatgagt catgcacaga ttcgtaacga catgagcaac aagcgtaaag
    77461 cagaagcacg ccaaaaagca gaacgtgcgg aaaacattaa gaaaaatgat aaaaacccag
    77521 cataaatggc gagggtttaa gcaatcgagc ggcagcgtac ttaccccgca gtccattagc
    77581 gggtatactc gccgcattgt cctcttagtt aaatggatat aacgagcccc tcctaagggc
    77641 taattgcagg ttcgattcct gcaggggaca ccatttatca gttcgctccc atccgtacca
    77701 gtccgcaaaa tcccctgaat atcaagcctt ccgtatattc acagttcgtc atggttcgcg
    77761 tcagatcgtt gacagccgca ctccatgacg ggtaaaaagt ggataaaata attttaccca
    77821 ccggattttt acccatgctc accgttaagc agattgaagc agcaaagccg aaagaaaaac
    77881 cataccgcct tctcgatggt aatggcctgt acctttatgt ccctgtatcc gggaaaaagg
    77941 tatggcagct tcgctacaag attgacggta aggagaaaa
//
LOCUS       DAJTUN010000002        75657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTUN010000002 DAJTUN010000000
VERSION     DAJTUN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.002"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..201)
                     /locus_tag="OO470_000078"
     CDS             complement(<1..201)
                     /locus_tag="OO470_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249472.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLH"
     gene            complement(203..423)
                     /locus_tag="OO470_000079"
                     /pseudo
     CDS             complement(203..423)
                     /locus_tag="OO470_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(416..637)
                     /locus_tag="OO470_000080"
     CDS             complement(416..637)
                     /locus_tag="OO470_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249473.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(627..827)
                     /locus_tag="OO470_000081"
     CDS             complement(627..827)
                     /locus_tag="OO470_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6249474.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            957..1136
                     /locus_tag="OO470_000082"
     CDS             957..1136
                     /locus_tag="OO470_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249475.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1261..1590
                     /locus_tag="OO470_000083"
     CDS             1261..1590
                     /locus_tag="OO470_000083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249476.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1640..1828)
                     /locus_tag="OO470_000084"
     CDS             complement(1640..1828)
                     /locus_tag="OO470_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6249477.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2203..3431)
                     /locus_tag="OO470_000085"
                     /pseudo
     CDS             complement(2203..3431)
                     /locus_tag="OO470_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3680..4801
                     /gene="roxA"
                     /locus_tag="OO470_000086"
                     /gene_synonym="ycfD"
     CDS             3680..4801
                     /gene="roxA"
                     /locus_tag="OO470_000086"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6249478.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4850..6076)
                     /gene="pepT"
                     /locus_tag="OO470_000087"
     CDS             complement(4850..6076)
                     /gene="pepT"
                     /locus_tag="OO470_000087"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6249479.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6326..7462
                     /gene="potA"
                     /locus_tag="OO470_000088"
     CDS             6326..7462
                     /gene="potA"
                     /locus_tag="OO470_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6249480.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7446..8303
                     /gene="potB"
                     /locus_tag="OO470_000089"
     CDS             7446..8303
                     /gene="potB"
                     /locus_tag="OO470_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6249481.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8300..9094
                     /gene="potC"
                     /locus_tag="OO470_000090"
     CDS             8300..9094
                     /gene="potC"
                     /locus_tag="OO470_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6249482.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9091..10137
                     /gene="potD"
                     /locus_tag="OO470_000091"
     CDS             9091..10137
                     /gene="potD"
                     /locus_tag="OO470_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6249483.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10293..11114)
                     /gene="cobB"
                     /locus_tag="OO470_000092"
     CDS             complement(10293..11114)
                     /gene="cobB"
                     /locus_tag="OO470_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6249484.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11130..12041)
                     /gene="nagK"
                     /locus_tag="OO470_000093"
     CDS             complement(11130..12041)
                     /gene="nagK"
                     /locus_tag="OO470_000093"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6249485.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12070..13314)
                     /gene="lolE"
                     /locus_tag="OO470_000094"
     CDS             complement(12070..13314)
                     /gene="lolE"
                     /locus_tag="OO470_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6249486.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13314..14015)
                     /gene="lolD"
                     /locus_tag="OO470_000095"
     CDS             complement(13314..14015)
                     /gene="lolD"
                     /locus_tag="OO470_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6249487.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14008..15207)
                     /gene="lolC"
                     /locus_tag="OO470_000096"
     CDS             complement(14008..15207)
                     /gene="lolC"
                     /locus_tag="OO470_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6249488.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15469..16542
                     /locus_tag="OO470_000097"
     CDS             15469..16542
                     /locus_tag="OO470_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6249489.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16670..20116
                     /gene="mfd"
                     /locus_tag="OO470_000098"
     CDS             16670..20116
                     /gene="mfd"
                     /locus_tag="OO470_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6249490.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20259..21221
                     /gene="ldtC"
                     /locus_tag="OO470_000099"
     CDS             20259..21221
                     /gene="ldtC"
                     /locus_tag="OO470_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6249491.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21304..21516)
                     /locus_tag="OO470_000100"
     CDS             complement(21304..21516)
                     /locus_tag="OO470_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6249492.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21801..22433
                     /gene="comR"
                     /locus_tag="OO470_000101"
     CDS             21801..22433
                     /gene="comR"
                     /locus_tag="OO470_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6249493.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22495..23034)
                     /locus_tag="OO470_000102"
     CDS             complement(22495..23034)
                     /locus_tag="OO470_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6249494.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23261..24565)
                     /gene="ndh"
                     /locus_tag="OO470_000103"
     CDS             complement(23261..24565)
                     /gene="ndh"
                     /locus_tag="OO470_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6249495.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24973..25515)
                     /locus_tag="OO470_000104"
     CDS             complement(24973..25515)
                     /locus_tag="OO470_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6249496.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25538..26563)
                     /gene="nagZ"
                     /locus_tag="OO470_000105"
     CDS             complement(25538..26563)
                     /gene="nagZ"
                     /locus_tag="OO470_000105"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6249497.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26574..27398)
                     /gene="thiK"
                     /locus_tag="OO470_000106"
     CDS             complement(26574..27398)
                     /gene="thiK"
                     /locus_tag="OO470_000106"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6249498.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27379..28020)
                     /gene="lpoB"
                     /locus_tag="OO470_000107"
     CDS             complement(27379..28020)
                     /gene="lpoB"
                     /locus_tag="OO470_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6249499.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28035..28412)
                     /locus_tag="OO470_000108"
     CDS             complement(28035..28412)
                     /locus_tag="OO470_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6249500.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28415..28774)
                     /gene="hinT"
                     /locus_tag="OO470_000109"
     CDS             complement(28415..28774)
                     /gene="hinT"
                     /locus_tag="OO470_000109"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6249501.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29113..31298
                     /gene="fhuE"
                     /locus_tag="OO470_000110"
                     /pseudo
     CDS             29113..31298
                     /gene="fhuE"
                     /locus_tag="OO470_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31358..32791)
                     /gene="ptsG"
                     /locus_tag="OO470_000111"
     CDS             complement(31358..32791)
                     /gene="ptsG"
                     /locus_tag="OO470_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6249502.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33086..33883)
                     /locus_tag="OO470_000112"
     CDS             complement(33086..33883)
                     /locus_tag="OO470_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6249503.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33894..34898)
                     /gene="holB"
                     /locus_tag="OO470_000113"
     CDS             complement(33894..34898)
                     /gene="holB"
                     /locus_tag="OO470_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6249504.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34895..35536)
                     /locus_tag="OO470_000114"
     CDS             complement(34895..35536)
                     /locus_tag="OO470_000114"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6249505.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35526..36548)
                     /gene="yceG"
                     /locus_tag="OO470_000115"
     CDS             complement(35526..36548)
                     /gene="yceG"
                     /locus_tag="OO470_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6249506.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36551..37360)
                     /gene="pabC"
                     /locus_tag="OO470_000116"
     CDS             complement(36551..37360)
                     /gene="pabC"
                     /locus_tag="OO470_000116"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6249507.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37480..38721)
                     /gene="fabF"
                     /locus_tag="OO470_000117"
     CDS             complement(37480..38721)
                     /gene="fabF"
                     /locus_tag="OO470_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6249508.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38808..39044)
                     /gene="acpP"
                     /locus_tag="OO470_000118"
     CDS             complement(38808..39044)
                     /gene="acpP"
                     /locus_tag="OO470_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6249509.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39255..39989)
                     /gene="fabG"
                     /locus_tag="OO470_000119"
     CDS             complement(39255..39989)
                     /gene="fabG"
                     /locus_tag="OO470_000119"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6249510.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40002..40931)
                     /gene="fabD"
                     /locus_tag="OO470_000120"
     CDS             complement(40002..40931)
                     /gene="fabD"
                     /locus_tag="OO470_000120"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6249511.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40947..41900)
                     /gene="fabH"
                     /locus_tag="OO470_000121"
     CDS             complement(40947..41900)
                     /gene="fabH"
                     /locus_tag="OO470_000121"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6249512.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41968..43038)
                     /gene="plsX"
                     /locus_tag="OO470_000122"
     CDS             complement(41968..43038)
                     /gene="plsX"
                     /locus_tag="OO470_000122"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6249513.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43119..43292)
                     /gene="rpmF"
                     /locus_tag="OO470_000123"
     CDS             complement(43119..43292)
                     /gene="rpmF"
                     /locus_tag="OO470_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6249514.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43344..43865)
                     /gene="yceD"
                     /locus_tag="OO470_000124"
     CDS             complement(43344..43865)
                     /gene="yceD"
                     /locus_tag="OO470_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6249515.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44064..44648
                     /gene="yceF"
                     /locus_tag="OO470_000125"
     CDS             44064..44648
                     /gene="yceF"
                     /locus_tag="OO470_000125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6249516.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44760..45719)
                     /gene="rluC"
                     /locus_tag="OO470_000126"
     CDS             complement(44760..45719)
                     /gene="rluC"
                     /locus_tag="OO470_000126"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6249517.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46292..49477
                     /gene="rne"
                     /locus_tag="OO470_000127"
     CDS             46292..49477
                     /gene="rne"
                     /locus_tag="OO470_000127"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6249518.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49673..50626)
                     /gene="flgL"
                     /locus_tag="OO470_000128"
     CDS             complement(49673..50626)
                     /gene="flgL"
                     /locus_tag="OO470_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6249519.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50638..52280)
                     /gene="flgK"
                     /locus_tag="OO470_000129"
                     /pseudo
     CDS             complement(50638..52280)
                     /gene="flgK"
                     /locus_tag="OO470_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52346..53287)
                     /gene="flgJ"
                     /locus_tag="OO470_000130"
     CDS             complement(52346..53287)
                     /gene="flgJ"
                     /locus_tag="OO470_000130"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6249520.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53287..54384)
                     /gene="flgI"
                     /locus_tag="OO470_000131"
     CDS             complement(53287..54384)
                     /gene="flgI"
                     /locus_tag="OO470_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6249521.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54396..55094)
                     /gene="flgH"
                     /locus_tag="OO470_000132"
     CDS             complement(54396..55094)
                     /gene="flgH"
                     /locus_tag="OO470_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6249522.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55147..55929)
                     /gene="flgG"
                     /locus_tag="OO470_000133"
     CDS             complement(55147..55929)
                     /gene="flgG"
                     /locus_tag="OO470_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6249523.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTQGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56067..56818)
                     /gene="flgF"
                     /locus_tag="OO470_000134"
                     /pseudo
     CDS             complement(56067..56818)
                     /gene="flgF"
                     /locus_tag="OO470_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56838..58046)
                     /gene="flgE"
                     /locus_tag="OO470_000135"
     CDS             complement(56838..58046)
                     /gene="flgE"
                     /locus_tag="OO470_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6249524.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(58071..58766)
                     /gene="flgD"
                     /locus_tag="OO470_000136"
     CDS             complement(58071..58766)
                     /gene="flgD"
                     /locus_tag="OO470_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6249525.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58778..59181)
                     /gene="flgC"
                     /locus_tag="OO470_000137"
                     /pseudo
     CDS             complement(58778..59181)
                     /gene="flgC"
                     /locus_tag="OO470_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59185..59601)
                     /gene="flgB"
                     /locus_tag="OO470_000138"
     CDS             complement(59185..59601)
                     /gene="flgB"
                     /locus_tag="OO470_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6249526.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59756..60415
                     /gene="flgA"
                     /locus_tag="OO470_000139"
     CDS             59756..60415
                     /gene="flgA"
                     /locus_tag="OO470_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6249527.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60491..60784
                     /gene="flgM"
                     /locus_tag="OO470_000140"
     CDS             60491..60784
                     /gene="flgM"
                     /locus_tag="OO470_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6249528.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60789..61205
                     /gene="flgN"
                     /locus_tag="OO470_000141"
     CDS             60789..61205
                     /gene="flgN"
                     /locus_tag="OO470_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6249529.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61245..62780)
                     /gene="murJ"
                     /locus_tag="OO470_000142"
     CDS             complement(61245..62780)
                     /gene="murJ"
                     /locus_tag="OO470_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6249530.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62947..63813)
                     /locus_tag="OO470_000143"
     CDS             complement(62947..63813)
                     /locus_tag="OO470_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6249531.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63815..64462)
                     /locus_tag="OO470_000144"
                     /pseudo
     CDS             complement(63815..64462)
                     /locus_tag="OO470_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64473..65057)
                     /gene="rimJ"
                     /locus_tag="OO470_000145"
     CDS             complement(64473..65057)
                     /gene="rimJ"
                     /locus_tag="OO470_000145"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6249532.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65293..66501
                     /gene="mdtH"
                     /locus_tag="OO470_000146"
     CDS             65293..66501
                     /gene="mdtH"
                     /locus_tag="OO470_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6249533.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66565..67212
                     /gene="grxB"
                     /locus_tag="OO470_000147"
     CDS             66565..67212
                     /gene="grxB"
                     /locus_tag="OO470_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6249534.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67346..67906
                     /locus_tag="OO470_000148"
     CDS             67346..67906
                     /locus_tag="OO470_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6249535.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68012..69058
                     /gene="pyrC"
                     /locus_tag="OO470_000149"
     CDS             68012..69058
                     /gene="pyrC"
                     /locus_tag="OO470_000149"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6249536.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69132..69377
                     /gene="dinI"
                     /locus_tag="OO470_000150"
     CDS             69132..69377
                     /gene="dinI"
                     /locus_tag="OO470_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6249537.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69667..69921
                     /gene="bssS"
                     /locus_tag="OO470_000151"
     CDS             69667..69921
                     /gene="bssS"
                     /locus_tag="OO470_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6249538.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70036..71154
                     /gene="solA"
                     /locus_tag="OO470_000152"
     CDS             70036..71154
                     /gene="solA"
                     /locus_tag="OO470_000152"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6249539.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71202..71315
                     /locus_tag="OO470_000153"
     CDS             71202..71315
                     /locus_tag="OO470_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6249540.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71576..72142
                     /locus_tag="OO470_000154"
     CDS             71576..72142
                     /locus_tag="OO470_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6249541.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72146..72721
                     /locus_tag="OO470_000155"
     CDS             72146..72721
                     /locus_tag="OO470_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6249542.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72763..73815)
                     /locus_tag="OO470_000156"
     CDS             complement(72763..73815)
                     /locus_tag="OO470_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6249543.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74040..74960
                     /gene="lpxL"
                     /locus_tag="OO470_000157"
     CDS             74040..74960
                     /gene="lpxL"
                     /locus_tag="OO470_000157"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6249544.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75132..75329
                     /locus_tag="OO470_000158"
     CDS             75132..75329
                     /locus_tag="OO470_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6249545.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75353..>75657
                     /locus_tag="OO470_000159"
     CDS             75353..>75657
                     /locus_tag="OO470_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6249546.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     "
BASE COUNT        18427 a        20028 c        18709 g        18493 t
ORIGIN      
        1 gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg ttattaatgc
       61 cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt caagccatat
      121 gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc ggtctattgt
      181 ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa ataacctctg
      241 tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg atttatattc
      301 tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg aaagcgcatc
      361 agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac gatcatcacg
      421 cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca ttcagtgacc
      481 gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc tgttcttctc
      541 cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc gtagtgagtg
      601 cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg acgaatacgg
      661 gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg ttccgtccaa
      721 gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg ccacacaaag
      781 gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt
      841 ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt gctttttgtg
      901 ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta attaacgtgt
      961 caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta gatcctgact
     1021 tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt gctgcctgga
     1081 taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa ggataaaaac
     1141 tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac acaatgacca
     1201 acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga aagaaagagc
     1261 atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt aaagaatact
     1321 catcaaagga gagaacttcg cagttatggc aacgcactga accagactga ttgcattgcc
     1381 caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac acacaaacgg
     1441 cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt tttatgcctg
     1501 aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc cgcccacgtt
     1561 ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga tgtgtggcgg
     1621 ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag gccagaacct
     1681 cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg aattgtccgg
     1741 ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg cattcttttt
     1801 cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta aagagttatt
     1861 tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga gttggcaaat
     1921 gttgaggccg catggtttac agaaagagga ataatcagga taaaatcctt taaattcatg
     1981 ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta aagagtgatt
     2041 cgctagtgta taaaaaatca gcaacacatt aaaaatcagc tatttataaa tctgtacact
     2101 ttttcacctc ttgttcgtag ttgttccgct ttgttgctca ttgttgcacg ttgtatctgt
     2161 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
     2221 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
     2281 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
     2341 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
     2401 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
     2461 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
     2521 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
     2581 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
     2641 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
     2701 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
     2761 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gttttttccc
     2821 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
     2881 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
     2941 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
     3001 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
     3061 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
     3121 taacgcttcc tgtacggtgt aattaagctc atgacgtgga ttttcccctc tgccagccat
     3181 gcgcgacact gggcggcttt ttccctggct gatttcaggt tcagatcagg ataatttccc
     3241 agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt gaaataccag
     3301 gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt aagaaaggct
     3361 gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag tttgttaagt
     3421 gctctgctca taattttgat tccggtcatc acaattaact acaccattaa ctacaccgat
     3481 cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac aaatctgttt
     3541 ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa cactaattga
     3601 taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac
     3661 cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg attttcttga
     3721 acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc
     3781 gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca gtcgactggt
     3841 cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct acgatcatct
     3901 cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg agccgaccgc
     3961 cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc tgatgatttc
     4021 tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat
     4081 tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg
     4141 cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga
     4201 gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa
     4261 tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa ttagcggatt
     4321 tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc
     4381 acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc gtgagatgat
     4441 gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt ttatatccca
     4501 gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag atgaaatcta
     4561 cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat
     4621 tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga
     4681 tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc
     4741 gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct tcgaagggta
     4801 agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct tacttccgtt
     4861 gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata ccttccagaa
     4921 tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc gggcaaggta
     4981 atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc agttccggtt
     5041 caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga tgctcaacca
     5101 ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag caatcaggat
     5161 gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc gcttcaaact
     5221 gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg gtgcctttca
     5281 tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt tcatccgccg
     5341 gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact cctttcgccg
     5401 tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg ttgaagtttt
     5461 caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg gcatcgaagg
     5521 catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta aaggcgacgc
     5581 gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc atgatttctg
     5641 caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta atcaacgtct
     5701 gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca tcgccgatac
     5761 ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca tttttgccgc
     5821 tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata tcgccaggga
     5881 cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca ttgataagcc
     5941 ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct tccgtgctgg
     6001 gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc aaaaatcgct
     6061 caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta agaagcaaga
     6121 aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt tatcagcatt
     6181 accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga ttcaagcggt
     6241 ttgccgtaga attaccgccc tcattaagag tcaccaaggt ggttaaccac aaaccccgca
     6301 tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata aacaaccgag
     6361 ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg gtaaagaggt
     6421 cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc
     6481 tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg ttgattccgg
     6541 acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc gctatgtgaa
     6601 cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa atgtggcctt
     6661 tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga tggaagccct
     6721 gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct ctggtggtca
     6781 acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag cctcgtctgt tgttgctgga
     6841 tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg agctgaaagc
     6901 gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg aagaagcact
     6961 caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag acggcacgcc
     7021 gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg gcgaaattaa
     7081 tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg ccaacgttga
     7141 aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa aactgcatgt
     7201 tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc acgctgaagg
     7261 gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt cggttgttga
     7321 actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg atcctgactt
     7381 tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg aggtcgtact
     7441 ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt gtcggttggc
     7501 ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt ttgacccgcg
     7561 acgacgccag tttcgtcaaa atggtcttta cgctggataa ctacacgcgt ctgctcgatc
     7621 cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc accctcgcct
     7681 gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc
     7741 cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt cgtatctacg
     7801 ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc tggctgggcg
     7861 ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc ggtctggttt
     7921 acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa ctggataagc
     7981 cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt atccgcatca
     8041 ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg ctgccagcga
     8101 tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg
     8161 tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc accagcatta
     8221 cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct cgtttgctga
     8281 ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta tgaccgctat
     8341 ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta acagctcgcg
     8401 ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga tgaacaacga
     8461 cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg cgacgtttgc
     8521 tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc gcggtaagcc
     8581 gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga tggcaatttc
     8641 gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc tgctgttctc
     8701 gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc tgaaaggttt
     8761 tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta ccattctgcg
     8821 aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt taagctttac
     8881 cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt atgaaattct
     8941 gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc
     9001 aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta ttgctcgtga
     9061 taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg
     9121 gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa cacgctgtat
     9181 ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac caaagaaacc
     9241 ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc caagctgaaa
     9301 acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt cgataaaatg
     9361 cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt cagcaatctc
     9421 gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat tccgtatatc
     9481 tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc tgtcaccagc
     9541 tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga cgatgcccgt
     9601 gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac cgatccgaaa
     9661 gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc agcgtttaac
     9721 tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat gatctggaac
     9781 ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg gccgaaagaa
     9841 ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa caaagaaggc
     9901 gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact
     9961 atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga agtggcaaac
    10021 gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca gaatgacgtt
    10081 ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg acgttaattc
    10141 agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg gctaatgagt
    10201 caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt gtaggcctga
    10261 taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa cttttcaaca
    10321 aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc attaccaacc
    10381 tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc ttcgtgtaca
    10441 aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac atccgccatc
    10501 gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc
    10561 gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg
    10621 gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata
    10681 tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc
    10741 aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc
    10801 tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca
    10861 ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg
    10921 ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc ccctgtcagt
    10981 acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc
    11041 tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa ccgatgaccc
    11101 cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca
    11161 cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt
    11221 aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa attcgataag
    11281 ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc cagagaaacc
    11341 gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa
    11401 agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca
    11461 aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt
    11521 aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac
    11581 tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag cccgccgcca
    11641 acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc atcatcccag
    11701 gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt
    11761 gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc ggcatacagc
    11821 ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca
    11881 aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg
    11941 tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca
    12001 agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat
    12061 gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc
    12121 cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc
    12181 cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg agaggaactg
    12241 atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg aaacaactac
    12301 gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa
    12361 gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact
    12421 cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac
    12481 cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc
    12541 gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg
    12601 taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga
    12661 acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag
    12721 ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg gctgcatcag
    12781 tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct gcttcacttt
    12841 cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg
    12901 ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct gttgtgggtt
    12961 aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac cggtgaaatt
    13021 gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca gtgcttcctg
    13081 ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca ccgccagaat
    13141 gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc
    13201 cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca tgccgccgcg
    13261 tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg ccattactcc
    13321 gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag ttggcggctc
    13381 atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc ctgcaagcga
    13441 ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta
    13501 ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat cgctacacgc
    13561 tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc
    13621 gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt gccaatcagc
    13681 agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg aaactgataa
    13741 ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag tttgctcatc
    13801 ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc taacaggtgc
    13861 agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc accttcgccc
    13921 acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg atagcgtttg
    13981 cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgtgt
    14041 ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag
    14101 cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc catcaagcag
    14161 gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc
    14221 tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga ccatcatgat
    14281 ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccatcac
    14341 catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca ggctcagcag
    14401 taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg
    14461 ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt caactctcag
    14521 cggctcatcc agccacaaac gccagccggt aatattggct gccggataac gcatcagacg
    14581 cgatgcatcc tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa
    14641 agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg tgaactggct
    14701 ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact gtgaggcaag
    14761 ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac
    14821 cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc
    14881 cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac
    14941 gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga
    15001 agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc
    15061 gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga
    15121 aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt aacgcaggcc
    15181 aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga
    15241 gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat
    15301 ctgaaaagcc cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc
    15361 ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata
    15421 accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat gaaacaaaaa
    15481 gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg
    15541 gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga agtcctgagc
    15601 aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt
    15661 atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat
    15721 aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg
    15781 ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc
    15841 gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg
    15901 tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg
    15961 ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg
    16021 tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg cgcatggttt
    16081 ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct
    16141 tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg
    16201 ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg attcggtatg
    16261 cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat
    16321 cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg
    16381 tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt
    16441 actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc
    16501 tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac cccagccggg
    16561 gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga
    16621 tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca
    16681 atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg
    16741 cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact
    16801 cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga
    16861 tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca
    16921 tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg
    16981 catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca
    17041 cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca
    17101 actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac
    17161 gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt
    17221 ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga
    17281 ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat
    17341 tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat
    17401 ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc agccattgtt
    17461 cttcagcgaa ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa
    17521 tactggcgat ctggaaacca gtgccgaacg tttccaagct gacacgctgg cgcgttttga
    17581 gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt
    17641 ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa ctgaacattt
    17701 accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca
    17761 ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc
    17821 ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg
    17881 aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta
    17941 tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc tggcgctgat
    18001 ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac
    18061 catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt
    18121 ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag ctggcggcat
    18181 tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc
    18241 gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa
    18301 acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt
    18361 ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa
    18421 acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa ccacgccgga
    18481 tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg
    18541 tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct
    18601 ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc ttgcgcagca
    18661 gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc
    18721 ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat
    18781 cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg
    18841 cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gcattaaagc
    18901 gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac gtacgctgaa
    18961 tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct
    19021 ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg
    19081 tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa acattcagaa
    19141 agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg gtcacgggca
    19201 gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt
    19261 gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat
    19321 cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg
    19381 acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag cgatgactac
    19441 cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc
    19501 gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag
    19561 cggttcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga
    19621 tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt
    19681 cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga acacgcgtct
    19741 gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt
    19801 cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc tggatattgc
    19861 ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg
    19921 cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct
    19981 gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga
    20041 tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga
    20101 gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc aacatttaca
    20161 aattctttgc actttcctgc actatccgac actgtcacca tccataattc aggcttatct
    20221 gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa acgcgttttt
    20281 ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag
    20341 ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac aaatttcatg
    20401 tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac gtcggctttc
    20461 tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt
    20521 taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt gtgatcaaca
    20581 ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa
    20641 taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc accgtttcag
    20701 acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt tataaagcac
    20761 agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg ggccatcatg
    20821 cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac gccgatttcg
    20881 gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat atcaaaacac
    20941 tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg ataaaagtct
    21001 ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca gagaagattg
    21061 atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca tttaaagatg
    21121 cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg
    21181 tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat aaaaaaaatc
    21241 cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa tgagggttaa
    21301 tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta cagaagtaat
    21361 acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt ccagcgatcc
    21421 cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt ctggcgttga
    21481 ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg ccgcagcgat
    21541 gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc gcaagaaatt
    21601 acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag atatttttta
    21661 taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa gcaattattt
    21721 ttatgggatc tggactggtg aacggaggtg attattttta taataacctt tcattcacag
    21781 tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa aaaagccgtg
    21841 gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa ttgttctggc
    21901 aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga gccaaagcgc
    21961 ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt ctcgaccgct
    22021 atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg
    22081 agtctgcgct ggctgactat tttgcggcca tcgccaactg ctttaccagc aaagacaccc
    22141 cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca ggagatatag
    22201 ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc
    22261 aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg gcagaattcc
    22321 ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac
    22381 tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa taagtcgcaa
    22441 aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc cttgttatac
    22501 tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac ccggatcgcg
    22561 gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat
    22621 tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct
    22681 ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg cccccacaac
    22741 agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc caccagcaac
    22801 agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac
    22861 tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga
    22921 aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca ctgccgctac
    22981 gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat tcacgggaac
    23041 acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca
    23101 gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag tctgaaggaa
    23161 tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg
    23221 cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg
    23281 gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat
    23341 gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca attcgtcctt
    23401 caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg gagaagttcg
    23461 acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc ttaccgttca
    23521 tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg
    23581 gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtaaatgt
    23641 ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttagttt
    23701 caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc cataccatca
    23761 gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga
    23821 ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg gccgcagtag
    23881 agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc gttacgttca
    23941 gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc gcgttgtgca
    24001 attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc
    24061 catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc tggtggaagc
    24121 gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct ggcgtattga
    24181 aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt ttacgttccg
    24241 gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt ttcgcttcac
    24301 gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc gcatgggcca
    24361 gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagtg
    24421 gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttacgtc
    24481 ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca
    24541 caatcttttt caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat
    24601 taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa atgttcttcg
    24661 ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta atcaagtttt
    24721 gttgcttaaa agataggtaa ctttaccggg ggagatcaaa aaatagagtt tattgtttat
    24781 tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg cgtttggtcg
    24841 tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg atgcggcgtg
    24901 gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg ttacctgatg
    24961 cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat gcggggagat
    25021 attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat gatgcaactc
    25081 ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa ccagacaacg
    25141 atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg catactcttc
    25201 cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt tgaagatcac
    25261 ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca ggccaacgcc
    25321 gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt ctttaagcag
    25381 atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta catccgggtc
    25441 aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt caaaaccgtg
    25501 taaatagata atcatcgctt cctcacataa gccagggtta gtgacctgct ttctcttcct
    25561 gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga gccgagtcca
    25621 tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt tctgccttga
    25681 tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag accaggatca
    25741 tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg
    25801 caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt tcctgacgca
    25861 aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca tcactgtaga
    25921 tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg
    25981 acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg
    26041 agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc attccggctt
    26101 catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga tcggcatgat
    26161 aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc gcaaagctga
    26221 tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct tgcgccagtt
    26281 tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt aagcgggtaa
    26341 aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc accagatgat
    26401 tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga tcatgatagt
    26461 tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca cgctcttccg
    26521 cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc tccttattgt
    26581 tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg atcgccggtt
    26641 tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg aaaccatcgc
    26701 ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata gtcattgacc
    26761 aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag cgcgatatca
    26821 ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg cactaaattt
    26881 ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc ccgcgctttg
    26941 cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc gctttgctgc
    27001 cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg ttgttgatgt
    27061 agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt taccgcgccg
    27121 ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg tgcaatgctt
    27181 gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg cgcatcagga
    27241 tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa actaccgcca
    27301 ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat
    27361 tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc agataatttc
    27421 gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt tgccagaggc
    27481 gctggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg ctttactacg
    27541 ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg acagttgctg
    27601 ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg tttcggtcgc
    27661 ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca gcaggacgct
    27721 acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca ttgcgccatt
    27781 ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt gctcaattgg
    27841 gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct gttgtggctc
    27901 ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac gttgccccac
    27961 acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca tttttgtcat
    28021 caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg
    28081 gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc
    28141 agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg
    28201 ggttcttgcc tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa
    28261 aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat
    28321 tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacacc
    28381 agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc gccagcattg
    28441 gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat
    28501 ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa
    28561 tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt
    28621 tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa
    28681 tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac
    28741 gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat
    28801 gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct
    28861 gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat
    28921 cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac
    28981 acgacaaata tgaatgcgta tatttctcat ttgcatttgc atttgcagca aaattattcg
    29041 cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac aacataaacc
    29101 aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca agtcatcacc
    29161 aaaccgtcac tacttgccgg ttgcatagca ctggcactat taccttctgc cgcttttgct
    29221 gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatggc
    29281 gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat gactcaacgt
    29341 gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca gcagttacaa
    29401 acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc ggattccgat
    29461 cgtgctcttt attattcccg cggattccag atcgataact atatggttga tggtatcccc
    29521 acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc actgtttgaa
    29581 cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg
    29641 gcaattaata tggttcgaaa acacgcgacc tgtcgtgaat ttaaaggcga tgtctcggcg
    29701 gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc actcaccgaa
    29761 gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc atggctggac
    29821 cgctacaata gtgaaaagac cttcttctcg ggcattgtcg atgctgattt aggcgatctt
    29881 acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc aacctggggc
    29941 ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgcgc acgcagtacc
    30001 gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac cctgaagcag
    30061 cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt cgaatttgac
    30121 agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct ggttgggcca
    30181 tacagtaatt atggacctgg ctttgatcat gtcggcggca ccggttggaa cagtggcaaa
    30241 cgtaaagttg atgcgctgga tttgttcgct gacggtagtt atgaattgtt tggtcgtcag
    30301 cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttcagt tcatgggcca
    30361 acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaagccg
    30421 actggtcatc acagagcctg gcgcaggacg ataccacaca tatgaaatcg ttatatgctg
    30481 ccactcgtgt cacccttgcc gatccgctgc atctgatcct cggcgcacgt tataccaact
    30541 ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc
    30601 tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct attttccagc
    30661 cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg
    30721 agctgggtct gaaatcggac tggatgaata gccgtctgac caccacgtta gccatcttcc
    30781 gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg
    30841 aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg
    30901 gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatctt gcagaggata
    30961 acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg ttcaccagct
    31021 atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgttaactgg caaaatcgcg
    31081 tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc
    31141 tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca
    31201 ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc tacggcgcac
    31261 cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag
    31321 ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg atgtactcat
    31381 ccatctcggt tttcaggtta tcggatttag taccgaaaat cgcctgaaca ccagaaccag
    31441 caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat
    31501 cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac
    31561 caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct tttgcatctt
    31621 cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga
    31681 tggtgtagta aacaatcgca taaccgatac cgacgatcgg taacagccac agtttgctgc
    31741 tgttaccaga cagaacgatg aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca
    31801 tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca
    31861 ggatcggcgc aacgaacatg aagaagaact cgatcggctc ggtgataccg gtcaggaacg
    31921 aggtcagcgc cgcggagatc ataataccgc ccactttcgc acggttttct ggtttagcag
    31981 agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag
    32041 acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct
    32101 gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt
    32161 gcagaccaaa cggtaccagg caacgttcga tgaaaccgta aatgccaaac gcaactaccg
    32221 ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa
    32281 tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc
    32341 gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca
    32401 tgtacgctgc aatcgcaccg gagataatcc ctccgagtac gccagtatcc gccaagtgtt
    32461 tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt
    32521 taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa
    32581 agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg
    32641 cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc
    32701 ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat ttaccgacct
    32761 tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag tatgggtgct
    32821 ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag
    32881 cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca
    32941 cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat
    33001 ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt
    33061 aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt
    33121 gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt
    33181 tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc
    33241 gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga
    33301 cataacgcgc agcatcgcgc agttgctccg cattacggaa ggtcacaatg ccggaaaagg
    33361 agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt
    33421 gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac
    33481 gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat
    33541 ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg
    33601 tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt
    33661 agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc
    33721 ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt
    33781 tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat
    33841 aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga
    33901 tgaggaaccg gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg
    33961 gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg aatgtggcaa
    34021 acatccccca gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc
    34081 agtcccggca catcaacatt ggtcacctgc gcagcgccat gatggcgttt tagcgcatcc
    34141 atcagcaacg ttgccagcca gtgtaaacgc gccggagctt gttcatgatt aagggccgcc
    34201 agcagcgaat accagtcgcc cgatggcacg ctatatgcca acgcctgaca caatgtttca
    34261 cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgccaggcga accggcgctt
    34321 aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaagtc
    34381 acggcgtact gttctggagg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt
    34441 gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct
    34501 tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc atcggttacc
    34561 cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt gacctcacgt
    34621 accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta gtaatcggga
    34681 tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc
    34741 ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc
    34801 ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt
    34861 ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc ttcacccagt
    34921 gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg
    34981 tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga
    35041 aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc
    35101 caacttccgg ggtaacatcg agatagagcg ttaagtctgg gcgaaaatcc ccgagaacag
    35161 catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct
    35221 gagtggaaag atcgtggcga tcgccaatca cccaggtgcc gttagccagc gcaggtttga
    35281 tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg
    35341 taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa
    35401 gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcgagcg
    35461 tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga
    35521 tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta
    35581 tgactggcaa gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga
    35641 tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc
    35701 gcacccggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct
    35761 gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc
    35821 tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca
    35881 ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg atttttatct
    35941 ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc
    36001 ttgtgaactc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat caaggtgtct
    36061 ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg
    36121 gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg
    36181 tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt
    36241 tccaacaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct
    36301 ttaaagtgag aaagatccgg ttcgatacgc agcagccatt gaaaaacccg tgggcgattg
    36361 atgatcttat cagcataaag ctgttcaccg agcgccagac gtccggtccc tggcttcagg
    36421 gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag
    36481 acgcccacac cagcggcgat acccagtacc accagcaata acaagataat caataacact
    36541 tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg
    36601 acgaaaaggt gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa
    36661 taaccatctc atctgcctgc aacgcctcct ccagcgaggc ttgcacttcg acaagctgat
    36721 aagaggattg tgccagtaaa cggatacaga attgtcgcat aatgccgtta acacctgcct
    36781 gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta gccgcacagc
    36841 attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa
    36901 gatgagagcg aatcaatact tgctcaagcc gattaagatg tttaatacct gcaagatgag
    36961 gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt
    37021 cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgctggtccg ctgttcaatg
    37081 tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca ccattttgct
    37141 gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca caggaaatca
    37201 tcaaccgctg acaagcatcc tgtagtcgca ggatatgcgc cgataacaaa ctgactttac
    37261 cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta
    37321 ctgccagcga ttcctgctta taaccgttaa ttaagaacat accggctcct tatcagagaa
    37381 ttgttagtgt ggcagcatgt tcactacgga acaagtcgga ataaaagcta agaaaaaagg
    37441 cccgcaagcg gaccttttat atgggtggaa aatgacaact tagatctttt taaagatcaa
    37501 agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac
    37561 ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccgggt tatccaggtt
    37621 gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag attctactgc
    37681 acccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca acacacggct
    37741 tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt
    37801 agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag
    37861 agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata
    37921 agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt
    37981 cgcgtgttcg tactcttcaa gtaccagcat accggcacca tcgcccagta cgaagccatc
    38041 acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc
    38101 acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg cgccacctgc
    38161 aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatggccaa tgttgtgcac
    38221 gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt
    38281 cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg
    38341 tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc
    38401 ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc
    38461 ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg
    38521 ttctttgcgc gagataatgt cctcacagtt aaaatccttt actaagccag caaatttcgt
    38581 tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag
    38641 agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac
    38701 aactacacga cgcttagaca cgtttgtcct ccaaggaggg aaaaatgatt ctagtgggac
    38761 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
    38821 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
    38881 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
    38941 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
    39001 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
    39061 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
    39121 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
    39181 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
    39241 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    39301 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    39361 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    39421 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    39481 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    39541 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    39601 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    39661 ttagtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    39721 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    39781 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttggca
    39841 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    39901 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    39961 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    40021 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    40081 aggccagtaa gcaccttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    40141 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    40201 cggatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac agaaactgtt
    40261 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    40321 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gccagcagct
    40381 ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc
    40441 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    40501 gcaataggcg catcatccag accgatgatt gccgccatag cgcccgtgcc ttccggtacg
    40561 gcttcttgca tgaacttacc gcgcatctca accagacgca ccgcatcagc gaaatcaatc
    40621 acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    40681 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    40741 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    40801 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    40861 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    40921 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcagagcc ccaagtgaat
    40981 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    41041 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc
    41101 accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    41161 ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt cgccgccagc
    41221 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    41281 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    41341 tcaccataac taccgtcggc atgcagatgg gtggaaatga ttcccggctc ttcagaggca
    41401 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    41461 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    41521 ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcacaggc tgctgcaacg
    41581 tcaaatgccg ggcaaccttt aatgtccaac atgctttgaa tctgacaagc tgcgctcggg
    41641 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    41701 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    41761 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    41821 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    41881 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    41941 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    42001 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    42061 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    42121 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    42181 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    42241 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    42301 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    42361 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    42421 gattgtttta agcaccgctg aggcatcccg aatactgtca agacccttta cttcttcttc
    42481 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    42541 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    42601 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    42661 ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    42721 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    42781 tgaactccca cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    42841 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    42901 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    42961 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    43021 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    43081 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    43141 ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg
    43201 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    43261 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    43321 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    43381 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    43441 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    43501 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    43561 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    43621 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    43681 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    43741 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    43801 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt
    43861 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    43921 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    43981 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    44041 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    44101 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacct gaggtcgacg
    44161 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    44221 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    44281 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    44341 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    44401 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctaagcgagg
    44461 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    44521 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    44581 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    44641 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcagacggcc
    44701 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    44761 tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    44821 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    44881 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    44941 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    45001 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    45061 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    45121 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc
    45181 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    45241 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    45301 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    45361 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    45421 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    45481 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    45541 agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata
    45601 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt
    45661 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    45721 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    45781 gttttgcacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    45841 cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta
    45901 taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga
    45961 aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg
    46021 tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat
    46081 atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa
    46141 aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg
    46201 cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag
    46261 tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca
    46321 gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga
    46381 aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga
    46441 accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact
    46501 aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat
    46561 taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg
    46621 caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat
    46681 gccaaataac ccgcgcgcag gtggcatttc tcgccgtatc gaaggcgatg accgtaccga
    46741 attaaaagaa gcactggcaa gccttgaact gccggaaggc atggggctta tcgtgcgcac
    46801 cgctggcgta ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gcctgaaaca
    46861 ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga
    46921 gagcaacgta atcgttcgcg ccttccgcga ttatttacgt caggatatcg gcgaaatcct
    46981 tatcgataac ccgaaagtcc tcgaactggc acgtcagcat atcgctgcat taggtcgccc
    47041 ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca
    47101 gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccat ccggtggttc
    47161 cattgttatc gacagcaccg aagcgttaac agccatcgac atcaactccg cacgcgcgac
    47221 ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagccg ccgatgagat
    47281 tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat
    47341 gacgccagta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga
    47401 ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgcca
    47461 gcgcctgagc ccatcactgg gtgaatccag ccatcacgtc tgcccgcgct gctccggtac
    47521 cggtaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtttga tcgaagaaga
    47581 agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta
    47641 tctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa acgcgtcagg acggtgttcg
    47701 ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac taccacgtgt tgcgcgtgcg
    47761 caaaggggaa gaaacgccga ccttaagcta catgctgccg aagctgcatg aagaagcgat
    47821 ggcgctgccg tctgaagaag agttcgctga acgtaagcgt cctgaacaac ctgcactggc
    47881 aacctttgcc atgccggatg taccgccagc gccaacccca gctgaacctg ccgcgactgt
    47941 cgtagcccca gcaccgaaag ctgcaacggc aacaccagca gctcctgcac aacctgggct
    48001 gttaagccgc ttcttcggcg cactgaaagc gctgttcagc ggtggtgaag aagccaaacc
    48061 gaccgagcaa ccaacaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa
    48121 acctcgtcag agcaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac
    48181 tgaaggcagc gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcaaac
    48241 tgccgagacg cgtgagagcc gtcagcaggc tgaggtaacg gaaaaagcac gtaccaccga
    48301 cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc
    48361 gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga
    48421 agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg
    48481 ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc
    48541 tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct
    48601 gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga
    48661 caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca
    48721 gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt
    48781 agcgtgcacg tctccggaat tggcctctgg caaagtctgg atccgctatc caattgtacg
    48841 tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat
    48901 ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga
    48961 agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt
    49021 tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac
    49081 cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccgg tggttgaacc
    49141 gcaggaagag acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga
    49201 acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga
    49261 aacggtggcc gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat
    49321 gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg
    49381 ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc
    49441 ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc
    49501 cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa aattcatcaa
    49561 atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aatgcccgcc ccggcgatcc
    49621 cagagcgggc agacaaaaca tacccagttt catgatatgt tttaaagcga gcttatttgt
    49681 tgagctggaa gagcgacaat ccctgcatat cggtaaatgc tttatacgat gcctgcaatg
    49741 ccgtttgctg catgatgtaa gatgaaatag ttgcattcca gtccacatca accagatcgc
    49801 tcatctgctg cgtttgccct aaagcgcgat cgctacctaa ggaatccagc gactccagtt
    49861 tgttcagctg cgtgcctaat tccgcgcgaa cggtaaggac attattcagc gagtttttca
    49921 gtccacggtt ggttttatcc aatgccgcag cggcgatttc tttatccgct tcgctatccg
    49981 cgaccggcgt tttcagggct gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag
    50041 aagcgctacc gtctggttcc gctaccgcgt tactggtaat actgtcgaaa attttgtcgc
    50101 ccgtatgtcc tatcaccatc gaacgcgaag catcgacctg ttgcttaata ctttctgttc
    50161 cgccaacata atcaccgtta gcctcactaa acggcgcagt ctctgtttta taaccggcaa
    50221 aaatgtagcg cccattaccg tcagtggtgt tcgccagatt cagcaactgg tcacgaagcc
    50281 cctgaatatc cgtagccagc gaggcccggt catcgtcact caaggtgcca ttgctggcgt
    50341 agacaatttt ttcctgagca ttctggatag cagtggtgac ctggctaagt acactctctt
    50401 ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct
    50461 gtgcctggga gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa gggttaacga
    50521 ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca ttctgcctga gaattggtga
    50581 tgccgcgcat gttttgctgg tacatcatct gggtactgaa acgcatttta tctcccctta
    50641 gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc
    50701 caggtaatac tgctgaaaac gttgcagatt tccgtactcc tcatcgagat tgacaccgga
    50761 aatcgactgc tgctgattgg aaagctgcgt caccacatta ccttgcgtgg tgctactggt
    50821 tttcaacgtc gcggttttat taccgatatc actcactaac gaggcataag cgtcgttaaa
    50881 ggatttcgca ccgcccaccg ttttactgtt gctttgcaga tccagcaggg cctgaccgtt
    50941 gcggttgtcg ctatcacccg catcttcttc gctcgccatc gcgattttcg cttcgtcggt
    51001 gattaataca tccatgttga cgatggcgtc acttactggt ttcagcgtga agctgtcgtt
    51061 aacggcaggc gttcctgtaa acgtcaactc cagaccatca aatgccactt taccgttggc
    51121 atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc actgattatt
    51181 atcgaacgag attttgtaat ctgtcgccag taccacggag gcatcagtta ccgtggcacc
    51241 gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac cgatagtaaa
    51301 gaaatcttca ccagcatcgc cgttagcatc aaatccggct ttgtgttggc tgttgaaagc
    51361 ctcggcaaat gccagcgcca gttgtccaag cgtattacgc gtctggtcca gatcctgaga
    51421 acggaatgtc agaatgccgc ccagcgaccc ggtattcagt aatttctccg ggatctcaat
    51481 attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg
    51541 aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat tggcatcgtg
    51601 atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg
    51661 ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct
    51721 gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga
    51781 tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg
    51841 gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgccgcc
    51901 gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt
    51961 gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg
    52021 gcggtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat aaacgtatca
    52081 tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc agcgcccaac
    52141 gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg
    52201 gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg
    52261 ttattgatca agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg
    52321 cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg ctgtaggttt
    52381 tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt ttacgggcat
    52441 agtgaggatc ggtagcatag cccgcgtcct gtaggacctg cgccccctgt tccgcactcg
    52501 cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg taatccgaca
    52561 atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc gcttcgccgt
    52621 tttcatattc agtcgtggtg atttcagtga cttgcccttt ccagttgcca gaggctttga
    52681 caccaaacag gttatagctt ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac
    52741 cagattcaag cgcagcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg
    52801 ccagttgggc gggcagcgag agttgcgcga ggaatgctct actgtcaccc ggcagcgaat
    52861 catcgtagtt acgtggtacg gccttttgca ccagctgcga aagcgtctgg ttttgataac
    52921 gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg
    52981 gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg
    53041 tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg tgctcgctgc
    53101 tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca
    53161 tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg ctcgctttcg
    53221 cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc
    53281 tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcaa
    53341 aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcacgca ccacgttatt
    53401 gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg
    53461 cgtttgtgga gtaacaacag tctgtccacc accaaacggt gtatctggct ggctgacatt
    53521 ggcctgacga ttaactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt
    53581 cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg
    53641 cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct gggaactgtt
    53701 gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc
    53761 acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag
    53821 ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc
    53881 accattggtg atccgtccac cgttcagttg gttaacctga acactgctac cgcctgcgga
    53941 ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac
    54001 gcccttaagc ggtgtcatca acaacgtacc tccacgcaag cttttggcat tacccatgga
    54061 agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac
    54121 cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag
    54181 ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc
    54241 agtgccatcc agccccacta ccaagccata gccaatcagt gagttttgcc ttaccccctg
    54301 aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat
    54361 taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag
    54421 aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc tacgtattca
    54481 atacgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat agtgcgtgga
    54541 ttaaccacgc ccgagaagcg aataaattcg gtaccctgat taatggcaat ctgtttttca
    54601 cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg
    54661 ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc accggaggct
    54721 tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac agtatcaaag
    54781 ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct ggcgctgacg
    54841 ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac
    54901 agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc gttggcgacg
    54961 ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt
    55021 atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata agtatgcgca
    55081 gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt
    55141 tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca ccgctttact
    55201 gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg ccacgttgac
    55261 gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc cctgcgtgct
    55321 ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa tgctctccag
    55381 tccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg gagctgcctg
    55441 gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta acgcattcgc
    55501 cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct gcccgttctg
    55561 atccacctgg aaagagccgt cacggatata ggctgatgag ccatctggca acattacctg
    55621 gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta ttggtctgcg acagattgcc
    55681 ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga
    55741 gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt gataaagcag
    55801 atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg ccaggttgtt
    55861 ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga tccataatga
    55921 actgatcata taatgtcctg tagtggattg ctgacaagca gagcagaagc acgcgacagt
    55981 gtgaatttcc cctcacccta accctctccc caaaggggcg agggaatagt ccgcgcgctt
    56041 ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt ggcacggcct
    56101 gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca
    56161 atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc
    56221 acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt ttctgcgctt
    56281 aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt caccagtttc
    56341 agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc cgagattgtg
    56401 ccatcggcag caatcgtgat ttccgcccct tccggcacag caattggccc cgcctcgcct
    56461 atcaccggat gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta
    56521 cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccatcctgc tgcaacgcaa
    56581 cgtccagcgg gcgcgaggtg taatccatct tgccgggcgt catgtctgca cccggtgttg
    56641 acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact gcgcgcaagg
    56701 cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg gcggttaccg
    56761 cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg tgatccattg
    56821 agctatcccg tcagcgatta gcgtaagtta acccgcgtgt tgaggatctg gtcctgggtt
    56881 ttgatggtct gggcgttaga cttatagtta cgctgggcaa cgatcatatt gaccagttct
    56941 ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagggtgcc aaagtttccc
    57001 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    57061 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    57121 tgggtttgtt cgttggaatt gttgccgaca accgtaccgt catcattgat ttgataactc
    57181 accagatcgc ccggtttgta gccattctgg gtggttgcca caatgttgtt agcgccggta
    57241 ttttgctgca tggagttgag gaagctcaga ctaaacgtgg cgggttctgc accgttaatt
    57301 gcgccggttg cgatattatt cgccatcgca ccatccacta atgtgccatt agcattaaat
    57361 tccagtgttg tcgctgtctt cgcaatgctg tttggatcac tgctatcctg ggtgtagacc
    57421 tgccagttat tatccccggt cttcacaaaa tagacgctca tgtcatgagc attaccctga
    57481 ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct ggcgctgaat
    57541 ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga cgccgtggtg
    57601 gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt cgccccttgc
    57661 tgaatagtcg gcggcgtacc ggttaccggg taacccgtca gctgtaaacc ttgcgtattc
    57721 aacaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa caccgaaccg
    57781 ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag acctcgcccg
    57841 gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt tacccccagt
    57901 cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt aaagccgtag
    57961 gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc agcgtttaat
    58021 ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc ttaaattatc
    58081 tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt attaccgctg
    58141 ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac cagttgtgta
    58201 ccaccgttac tggcgctaat cgctacattg taagaaccgt tcggcgcagt gctgccatca
    58261 gtcaacgaac cgtcccaggt gaaactgtga actccggcgg tcagttcacc aatatcaatg
    58321 gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc tgccgcctgt
    58381 tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact gccggttccc
    58441 gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga tcaggttact ggcctgtaac
    58501 gactggctgt tatcaatctg tccggaaata gatccgagcg tggtattgag tttttcaatc
    58561 ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc cattggattg
    58621 gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca gaaaactgct ttgtaaatct
    58681 gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt atttgtcgga
    58741 tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc gtaagggttt
    58801 tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag ctgcgtgacg
    58861 ccgacatggt gttaaccatc tccccgacga catcaacgtt cggcattttt acgtagccct
    58921 ttgcatctgc cagcggatta cctggttcat aaaccagttt gtccggggcc tgactttctt
    58981 aacatcggca acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac
    59041 cacctgtttt gcccgatatg gctgtccatc aggaccggtc acgctatcag catttgccag
    59101 attactggcc gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc
    59161 aaaaatattc agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt
    59221 gatttgcccg ctcaacgcgc taaggctcat ctggtattgc aggctgttat cggcaaactg
    59281 ggtgcgttcg cgatccatat cgacggtatt accgtcaagc gaaggctggt ccggaatacg
    59341 gtattgcagt tctgcggtag gaggcgtcag cgcctgcgcc gggatgtgtt gcgttgaggt
    59401 catcgtcagt gcaaccacac tggttgcatc ccgtccacgt tgcatgactt ttttaagttc
    59461 actggcaaaa tcgatatcgc gcgcctgata accaggggta tcggcattgg cgatgtttgc
    59521 tgccagcact tcctgacgct gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc
    59581 ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca aaattctgcc atcagcttaa
    59641 atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt
    59701 tgatggtcat tgcggacagg tacaattcac gttgtagaaa tggctggggg cgaaaatgct
    59761 gacaataaaa cgtagcgtgg cgatcatcgc gatactgttc agtccgttaa gtacggcgag
    59821 caatctcaca tcgcaattgc acaacttttt tagcgcccaa ctcgcggggg taagtgatga
    59881 ggttcgtgtt tctattcgta cagcgcccaa tctactaccg ccatgcgagc agccattgct
    59941 ttcgatgagc aataattccc gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa
    60001 cgacaaacga tatttacagg ttaatgtgca ggccacagga aattatgtgg ttgccgcgat
    60061 gcctattgtg cggggaggaa agctggaagc tggcaatgtc aaactgaaac gtggacgcct
    60121 ggataccctg ccaccgcgta cggtgctgga tatcaatcaa cttgttgatg ccgttagcct
    60181 gcgcgatcta tcacccgatc aacctatcca gttaacccag tttcgccagg catggcgggt
    60241 aaaagcggga caacgcgtca atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga
    60301 aggtcaggcg ctgaacaatg cagccgtcgc acagaatgcg cgggtgcgca tggtatcggg
    60361 acaggtagtc agcggcgttg ttgatgcaga tgggaatatt cttataaacc tgtaagctgt
    60421 taaagattac ccgtcccttg ccgataaata agcaacacat gataaaagcg ccctcaatga
    60481 ggaataaacc atgagtattg atcgcacttc gcctctgaag cctgtaagca ccgttcaacc
    60541 gcgcgaaacc accgacgcgc cggtaacgaa cagccgggcg gcaaaaacaa ccgcctccac
    60601 cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga
    60661 tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat
    60721 ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa
    60781 ctgatcgtat gacacgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc
    60841 tcaaaacggt aatggatcaa gagcagcaac atctctctat ggggcagatc aacggcagcc
    60901 agttgcaatg gattacagaa caaaaaagct cactgctggc gacgctggat tacctcgaac
    60961 agttacgcaa gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg
    61021 aaattactgt gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg
    61081 aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga
    61141 cgctatatgg ggcgaacggt cagacctcaa caccccatcg cggcagtaaa aagatttcga
    61201 tctgaatatt atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg
    61261 gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc
    61321 gccagcacga ccgccattaa acgcagtaag cgccagggca tggtacccaa tgaccactcc
    61381 ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga
    61441 cgcaacagaa acgccatcca gccgggttgc ggagtaaaga ttttctgctt acgcaactgc
    61501 cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca
    61561 tgtttcaacg gaccaataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg
    61621 gcaattttca ctggcgtttt aatgtcctgg cgggaataaa agccaggagc caacactttc
    61681 actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc
    61741 gcatcaaacg cggtaaattt accgtactgg aacagcgaaa cggttaacgg acctgaaaga
    61801 atgcccaacg caaccgcact cggcagtgcc aacaggaaac aaagacgcaa cccccagtcc
    61861 atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc
    61921 aaaatggtgc caagcgccac gcccagcaca ccggagggaa actccattaa gcggtcggcg
    61981 taatacatcc aagacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt
    62041 aaggagatct ggctcacaga gacgccaagg atcgccggtc ccatctgttt caccacgcgc
    62101 attgctccag catcgtggaa gttaatgcgc ggcaggacca gcatgccgat cttcttcagg
    62161 tgcggtagct gatacaccag ctgcaggatg ccgccgaccg taacagccca cgccagcgcc
    62221 agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta
    62281 agcagtgttg gcgcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc
    62341 accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag tagctggctg
    62401 gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac
    62461 ggtgcggcga gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa
    62521 acataagaga caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga
    62581 atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga
    62641 agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca
    62701 attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc
    62761 agcgatttta ataaattcat cggtgttcta atccggaccc acgggcaaaa cgcctgcaag
    62821 gcaggcgctg aaagaggcga atagtctacc tagattatgg tgaattgcta ccgccagatg
    62881 ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag
    62941 cactgcctat tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt caatgaagtg
    63001 ccgcgcacag ccgacaaatc cacgttgctc aagagtgctc tgccaaccag gaatcggttt
    63061 attcactaca ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag
    63121 cgcaccgtca gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca
    63181 agtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc
    63241 gtcgttagtc agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc
    63301 ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac
    63361 gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa
    63421 ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg
    63481 cgccgccagt tccaccagcc tttcagcatc gcgcagattt tctgccagcg gtttatcgac
    63541 acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtggc tggcggtgct
    63601 ggagtgcaca aaaaccgcat cacaactggc ggcaaggctg gataacgaat cggcataagg
    63661 aatgcgccag ctgtcacaaa ttggcagggc tttggcgcgc gtaggcgacc aggctccttg
    63721 taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc
    63781 taatcccact acgccgatac gtaatttttt cactttaatc tcccagatgg gccagcaacg
    63841 aatcaagacg ttgtttcagt tctgccactt cgatttccag ggcttcgacg cgggtctata
    63901 aatcaccgtc aaccatgttc gacatagccg ttaccgccgg ctgatcttca acctcaccac
    63961 tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa
    64021 aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga ctccacttcc gccatatcgc
    64081 tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca
    64141 ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg ccaaattctg
    64201 aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc actgtgcgta
    64261 gataatgacg tttgaccaga ttatccagct gttcctgcac ttcggattca ctcagattca
    64321 tgaccggttt acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg
    64381 ggtattgctc cggcgtggtc acctgctttt ccagcaagca accaattacg cgagcttcca
    64441 aggcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta
    64501 gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca
    64561 tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc
    64621 atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg
    64681 gtcagggctt caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga
    64741 tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct
    64801 ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta
    64861 atcatcccta gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc
    64921 tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca
    64981 cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta
    65041 ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag
    65101 tattactata cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta
    65161 ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atattgtccc
    65221 cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc
    65281 ccgggagggg aaatgtcccg cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc
    65341 atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc
    65401 ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccag
    65461 tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa
    65521 ccgatgattg ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc
    65581 cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt
    65641 gatccgccgc gttcggcgct ggtggtgaaa ctaatccgtc cacaacaacg tggtcgtttt
    65701 ttctcgctgt tgatgatgca ggacagtgcc agtgcggtca ttggcgcatt gttggggagc
    65761 tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgcta
    65821 tgtgcggcgt tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc
    65881 gttcgcgaag gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg
    65941 ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac
    66001 gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg
    66061 ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg
    66121 ttgatggctg ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc
    66181 ctacaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct
    66241 gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg
    66301 tttagccgtc tgggtctggc gattggcggc gctattggtt atatcggtgg tggctggctg
    66361 tttgacctgg gcaaatcggc gcaccagcca gagcttccgt ggatgatgct gggaattatt
    66421 ggcatcttca ctttccttgc gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt
    66481 ttgctggaac gcgacgcctg atttgatact cccccctgac tcctccatac tgaaagtgaa
    66541 ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc
    66601 ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc
    66661 aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa
    66721 aaagatgaca gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc
    66781 gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag
    66841 gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag
    66901 ttttctactc ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat
    66961 tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttgcgt
    67021 gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat
    67081 attcagctat ttccgctact gcgtaatctg acgctggtag ccggaatcaa ctggccaagc
    67141 cgcgttgcta attaccgcga taatatggcg aaacagacac aaatcaattt gttatcatca
    67201 atggcgattt aatcctgacc cgccgcggca gcctgtattc gcgggttgcc gccgttttca
    67261 gttttttctg ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga
    67321 cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt
    67381 cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat
    67441 taaccagtcg cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc
    67501 tgacgcccat attgttctga caaacttgac cagccaaatt ggtcgcgaag agccgaataa
    67561 ggttacccta accggagacg ccaatctgga catgaactcc ctgttcggta gtcagaaagc
    67621 gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt
    67681 cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat
    67741 gcaaacgttg cttccctatt tgaaccaggc attacgcaat tactttaacc agcaacctgc
    67801 ttacgtcctg cgcgaagatg gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg
    67861 cattgaagtg aagccaggcg aaattgtcat tccgtttacg gattaatgac gagggcgcat
    67921 tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa
    67981 aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat
    68041 ccgccgccca gacgactggc accttcacct ccgcgatggc gacatgttaa aaactgtcgt
    68101 gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt
    68161 gaccacagtt gaggctgccg tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg
    68221 gcacgatttc accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct
    68281 ggagcgcgga tttaatgaag ccgtgttcac cgctgcaaaa ctttacccgg caaacgcaac
    68341 cactaactcc agccacggcg tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat
    68401 ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat
    68461 ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac
    68521 tgcgctgaaa gtcgtttttg agcacatcac caccaaagat gctgccgact atgtccgtga
    68581 cggaaatgaa cggctggctg ccaccatcac tccgcagcat ctgatgttta accgcaacca
    68641 tatgctggtt ggtggcgtgc gtccgcacct gtattgttta cccatcctca aacgcaatat
    68701 tcaccaacag gcattgcgtg aactggtcgc cagcggtttt aatcgagtat tcctcggtac
    68761 ggattctgcg ccacatgcac gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt
    68821 caacgcccca accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctctgca
    68881 gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga
    68941 cacattcatc gaactggtac gtgaagagca acaggttgct gaaagcatcg cactgactga
    69001 tgacaccctg gtgccattcc tcgccgggga aacggtacgc tggtccgtta aacaataaaa
    69061 atcgtagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag tatgttcaac
    69121 agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg
    69181 ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg
    69241 aaggccacgt atcggtacgt tatgccgcag cgaataattt atcggttatt ggcgcaacaa
    69301 aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact
    69361 ggtttgtcag cgaataatat gcagtgattt ttgtttttgc cggatcgccc cggctttttt
    69421 attttactta cgaattttga gtacaaaatt tctacacttc ctcaaaataa ttgcacaata
    69481 tgctaaaaaa atatgaatga catgctgttt tattgttcat ataaatctta aaagcagaaa
    69541 tttatctgtt ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt
    69601 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
    69661 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
    69721 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
    69781 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
    69841 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
    69901 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
    69961 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
    70021 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
    70081 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg
    70141 ccaccgcatc aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt
    70201 gaaggcgaaa agtatgtccc gttggtcctc cgcgcgcaaa cgctgtggga tgaactttcc
    70261 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct
    70321 gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa
    70381 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
    70441 atcggcttat ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg
    70501 atccaactgg cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt
    70561 cgtcatgacg atgatggcgt aactattgaa acggttgacg gtgagtatca ggcgaaaaaa
    70621 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc
    70681 gtacgtaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc
    70741 ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac
    70801 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
    70861 gttccgtttg cggaagtggc cagtgatggg tcagaagcct tcccgttctt gcgcaatgta
    70921 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
    70981 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
    71041 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
    71101 gaccaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
    71161 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
    71221 atctcatcaa taatattcgc gagcatttga tgctttatct ttttctctgg ggattgttgg
    71281 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca
    71341 aatatttaat ctccctttca tttagcgtga aaaattgaat gatattattc ctgcttctac
    71401 agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat
    71461 taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa
    71521 ttcattctcc gacacgccat tttctttctc tcgcaaattc gtattggatg cacttatgtc
    71581 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc
    71641 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
    71701 tggctggtat cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg
    71761 gctggttatt cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta
    71821 ttcgcccatg actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt
    71881 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
    71941 tggctggttt gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg
    72001 tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tctgttatgc acggatttat
    72061 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
    72121 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc
    72181 cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
    72241 gcacgccttt gttaacttcc gcatacagca tcttggctat agctggttat atggcacctt
    72301 taaagatttc gacggtactt ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa
    72361 tgtgacaatt aacaccacca gcgtcgatac taaccacgcc gaacgcgata aacatcttcg
    72421 cagtgcggat tttctcaata ccacaaaata tccacaggca acattcacct ccaccagcgt
    72481 gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa
    72541 acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg gcggtaaacg
    72601 tgcaggcttt gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt
    72661 aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
    72721 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
    72781 aatacccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
    72841 acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat
    72901 ctcactacaa caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg
    72961 gcagccatca tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg
    73021 cgcgataatc tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
    73081 aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc
    73141 ctcgatatgc cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca
    73201 acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc
    73261 gactgctttt ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa
    73321 gtgccccact tcatactcat agtggttacg catgtcgata aatagtgcat cgggatcgtc
    73381 aagcatggcg ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg
    73441 aggatcgtca ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa
    73501 ggatttcccg tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa
    73561 ggcatagagc tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt
    73621 aatgccctca tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata
    73681 taaagcgtca cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt
    73741 ggttcgcggt tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt
    73801 gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg
    73861 atatagcaat tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa
    73921 ttatttgtta tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc
    73981 gggcgaaaaa atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata
    74041 tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt
    74101 tgggtattgg cgtactttgg ttggtcgtgc aattgcccta cccgattatc taccgcctcg
    74161 gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc
    74221 gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga
    74281 agaatttcga atccgttggc atgggcctga tggaaaccgg tatggcgtgg ttctggccgg
    74341 accgccgaat cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg
    74401 cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag ctgggcgcgc
    74461 ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga
    74521 ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag
    74581 atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccagatc
    74641 atgattacgg cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga
    74701 ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc
    74761 cacgccgtaa gccagatggc aaagggtatc aattgattat gctgccacca gagtgttctc
    74821 cgccactgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca
    74881 tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag
    74941 gcgttccttc acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta
    75001 ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc
    75061 gggcgcataa ttagtgtgtt tatcttttct tcttatgttc accgcgcctg gcgcaccaac
    75121 agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga
    75181 tcgtcgcctg gctaggctgt tttcttaccg gggccgcctt cagtctggta atgcccttct
    75241 tacccctcta cgttgagcag cttggtgtta ccggccactc cgccctgaat atgtggtccg
    75301 gtattgtctt cagtataatg ggttcctaaa cacatcattt taggtattga atatgagacg
    75361 ggcaatcagc tacatcagat tttcaagtga acgacaactg aaaggggact ctgtacgccg
    75421 acaatctaag ctggttactg actggcttga taagaaccct gagttttact tggattcatc
    75481 cttgagcttt aaagatttgg ggaaaagtgc ttttagtggt aagcacctga aaggtggtct
    75541 aggtgacttt ttaaccgcta tcgagaaagg gcttgttaaa gctggtgata cgctactaat
    75601 cgagagttta gaccgtctgt cacgtcagga tattgatatt gcttctgagc tattacg
//
LOCUS       DAJTUN010000003        72814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTUN010000003 DAJTUN010000000
VERSION     DAJTUN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.003"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OO470_000160"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OO470_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6249547.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OO470_000161"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OO470_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6249548.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OO470_000162"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OO470_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6249549.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OO470_000163"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OO470_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6249550.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OO470_000164"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OO470_000164"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6249551.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OO470_000165"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OO470_000165"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6249552.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OO470_000166"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OO470_000166"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6249553.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="OO470_000167"
     CDS             complement(4523..5245)
                     /locus_tag="OO470_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6249554.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OO470_000168"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OO470_000168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6249555.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="OO470_000169"
     CDS             6445..6663
                     /locus_tag="OO470_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6249556.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OO470_000170"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OO470_000170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6249557.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="OO470_000171"
     CDS             complement(7397..7597)
                     /locus_tag="OO470_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6249558.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7607..9344)
                     /gene="kefB"
                     /locus_tag="OO470_000172"
                     /pseudo
     CDS             complement(7607..9344)
                     /gene="kefB"
                     /locus_tag="OO470_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9344..9895)
                     /gene="kefG"
                     /locus_tag="OO470_000173"
     CDS             complement(9344..9895)
                     /gene="kefG"
                     /locus_tag="OO470_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6249559.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10026..11939
                     /locus_tag="OO470_000174"
     CDS             10026..11939
                     /locus_tag="OO470_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6249560.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            11939..12961
                     /locus_tag="OO470_000175"
     CDS             11939..12961
                     /locus_tag="OO470_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6249561.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            12955..13173
                     /locus_tag="OO470_000176"
     CDS             12955..13173
                     /locus_tag="OO470_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6249562.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13227..14096
                     /locus_tag="OO470_000177"
     CDS             13227..14096
                     /locus_tag="OO470_000177"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6249563.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14151..14555)
                     /locus_tag="OO470_000178"
     CDS             complement(14151..14555)
                     /locus_tag="OO470_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6249564.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14857..15489
                     /gene="crp"
                     /locus_tag="OO470_000179"
     CDS             14857..15489
                     /gene="crp"
                     /locus_tag="OO470_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6249565.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15540..17630
                     /locus_tag="OO470_000180"
     CDS             15540..17630
                     /locus_tag="OO470_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6249566.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17697..18917)
                     /gene="argD/dapC"
                     /locus_tag="OO470_000181"
     CDS             complement(17697..18917)
                     /gene="argD/dapC"
                     /locus_tag="OO470_000181"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6249567.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19003..19566)
                     /gene="pabA"
                     /locus_tag="OO470_000182"
     CDS             complement(19003..19566)
                     /gene="pabA"
                     /locus_tag="OO470_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6249568.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19598..20200)
                     /locus_tag="OO470_000183"
     CDS             complement(19598..20200)
                     /locus_tag="OO470_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6249569.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20190..20357)
                     /locus_tag="OO470_000184"
     CDS             complement(20190..20357)
                     /locus_tag="OO470_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6249570.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20462..21043)
                     /gene="ppiA"
                     /locus_tag="OO470_000185"
                     /gene_synonym="rotA"
     CDS             complement(20462..21043)
                     /gene="ppiA"
                     /locus_tag="OO470_000185"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6249571.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21314..22495
                     /gene="tsgA"
                     /locus_tag="OO470_000186"
     CDS             21314..22495
                     /gene="tsgA"
                     /locus_tag="OO470_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6249572.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22757..25300
                     /gene="nirB"
                     /locus_tag="OO470_000187"
     CDS             22757..25300
                     /gene="nirB"
                     /locus_tag="OO470_000187"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6249573.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25297..25623
                     /gene="nirD"
                     /locus_tag="OO470_000188"
     CDS             25297..25623
                     /gene="nirD"
                     /locus_tag="OO470_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6249574.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25749..26555
                     /gene="nirC"
                     /locus_tag="OO470_000189"
     CDS             25749..26555
                     /gene="nirC"
                     /locus_tag="OO470_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6249575.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26574..27947
                     /gene="cobA"
                     /locus_tag="OO470_000190"
     CDS             26574..27947
                     /gene="cobA"
                     /locus_tag="OO470_000190"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6249576.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28194..28361
                     /locus_tag="OO470_000191"
     CDS             28194..28361
                     /locus_tag="OO470_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6249577.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28656..29993
                     /gene="frlA"
                     /locus_tag="OO470_000192"
                     /pseudo
     CDS             28656..29993
                     /gene="frlA"
                     /locus_tag="OO470_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30014..31036
                     /gene="frlB"
                     /locus_tag="OO470_000193"
     CDS             30014..31036
                     /gene="frlB"
                     /locus_tag="OO470_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6249578.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31086..31916
                     /gene="frlC"
                     /locus_tag="OO470_000194"
     CDS             31086..31916
                     /gene="frlC"
                     /locus_tag="OO470_000194"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6249579.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31913..32698
                     /gene="frlD"
                     /locus_tag="OO470_000195"
     CDS             31913..32698
                     /gene="frlD"
                     /locus_tag="OO470_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6249580.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32798..33529
                     /gene="phoB"
                     /locus_tag="OO470_000196"
     CDS             32798..33529
                     /gene="phoB"
                     /locus_tag="OO470_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6249581.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33689..34774)
                     /locus_tag="OO470_000197"
     CDS             complement(33689..34774)
                     /locus_tag="OO470_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6249582.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34786..36090)
                     /locus_tag="OO470_000198"
     CDS             complement(34786..36090)
                     /locus_tag="OO470_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6249583.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36102..36455)
                     /locus_tag="OO470_000199"
     CDS             complement(36102..36455)
                     /locus_tag="OO470_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6249584.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36466..37344)
                     /locus_tag="OO470_000200"
     CDS             complement(36466..37344)
                     /locus_tag="OO470_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6249585.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37341..38567)
                     /locus_tag="OO470_000201"
     CDS             complement(37341..38567)
                     /locus_tag="OO470_000201"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6249586.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38568..39731)
                     /locus_tag="OO470_000202"
     CDS             complement(38568..39731)
                     /locus_tag="OO470_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6249587.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39815..40177)
                     /locus_tag="OO470_000203"
     CDS             complement(39815..40177)
                     /locus_tag="OO470_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6249588.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40194..41099)
                     /locus_tag="OO470_000204"
     CDS             complement(40194..41099)
                     /locus_tag="OO470_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249589.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41369..42373)
                     /gene="trpS"
                     /locus_tag="OO470_000205"
     CDS             complement(41369..42373)
                     /gene="trpS"
                     /locus_tag="OO470_000205"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6249590.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42366..43124)
                     /gene="gph"
                     /locus_tag="OO470_000206"
     CDS             complement(42366..43124)
                     /gene="gph"
                     /locus_tag="OO470_000206"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6249591.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43117..43794)
                     /gene="rpe"
                     /locus_tag="OO470_000207"
     CDS             complement(43117..43794)
                     /gene="rpe"
                     /locus_tag="OO470_000207"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6249592.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43812..44648)
                     /gene="dam"
                     /locus_tag="OO470_000208"
     CDS             complement(43812..44648)
                     /gene="dam"
                     /locus_tag="OO470_000208"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6249593.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44755..46041)
                     /gene="damX"
                     /locus_tag="OO470_000209"
     CDS             complement(44755..46041)
                     /gene="damX"
                     /locus_tag="OO470_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6249594.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46133..47221)
                     /gene="aroB"
                     /locus_tag="OO470_000210"
     CDS             complement(46133..47221)
                     /gene="aroB"
                     /locus_tag="OO470_000210"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6249595.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47278..47799)
                     /gene="aroK"
                     /locus_tag="OO470_000211"
     CDS             complement(47278..47799)
                     /gene="aroK"
                     /locus_tag="OO470_000211"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6249596.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48200..49438)
                     /gene="hofQ"
                     /locus_tag="OO470_000212"
     CDS             complement(48200..49438)
                     /gene="hofQ"
                     /locus_tag="OO470_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6249597.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49350..49754)
                     /gene="hofP"
                     /locus_tag="OO470_000213"
     CDS             complement(49350..49754)
                     /gene="hofP"
                     /locus_tag="OO470_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6249598.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49744..50184)
                     /gene="hofO"
                     /locus_tag="OO470_000214"
     CDS             complement(49744..50184)
                     /gene="hofO"
                     /locus_tag="OO470_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6249599.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50168..50707)
                     /gene="hofN"
                     /locus_tag="OO470_000215"
     CDS             complement(50168..50707)
                     /gene="hofN"
                     /locus_tag="OO470_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6249600.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50707..51486)
                     /gene="hofM"
                     /locus_tag="OO470_000216"
     CDS             complement(50707..51486)
                     /gene="hofM"
                     /locus_tag="OO470_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6249601.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51606..54158
                     /gene="mrcA"
                     /locus_tag="OO470_000217"
     CDS             51606..54158
                     /gene="mrcA"
                     /locus_tag="OO470_000217"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6249602.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54323..54883)
                     /gene="nudE"
                     /locus_tag="OO470_000218"
     CDS             complement(54323..54883)
                     /gene="nudE"
                     /locus_tag="OO470_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6249603.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55203..57338
                     /gene="igaA"
                     /locus_tag="OO470_000219"
     CDS             55203..57338
                     /gene="igaA"
                     /locus_tag="OO470_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6249604.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57403..58071
                     /gene="yrfG"
                     /locus_tag="OO470_000220"
     CDS             57403..58071
                     /gene="yrfG"
                     /locus_tag="OO470_000220"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6249605.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58082..58483
                     /gene="hslR"
                     /locus_tag="OO470_000221"
     CDS             58082..58483
                     /gene="hslR"
                     /locus_tag="OO470_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6249606.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58508..59377
                     /gene="hslO"
                     /locus_tag="OO470_000222"
     CDS             58508..59377
                     /gene="hslO"
                     /locus_tag="OO470_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6249607.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59521..61245)
                     /locus_tag="OO470_000223"
     CDS             complement(59521..61245)
                     /locus_tag="OO470_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6249608.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61624..63246
                     /gene="pckA"
                     /locus_tag="OO470_000224"
     CDS             61624..63246
                     /gene="pckA"
                     /locus_tag="OO470_000224"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6249609.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63322..64674)
                     /gene="envZ"
                     /locus_tag="OO470_000225"
     CDS             complement(63322..64674)
                     /gene="envZ"
                     /locus_tag="OO470_000225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6249610.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64671..65390)
                     /gene="ompR"
                     /locus_tag="OO470_000226"
     CDS             complement(64671..65390)
                     /gene="ompR"
                     /locus_tag="OO470_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6249611.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65618..66094
                     /gene="greB"
                     /locus_tag="OO470_000227"
     CDS             65618..66094
                     /gene="greB"
                     /locus_tag="OO470_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6249612.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66191..68512
                     /locus_tag="OO470_000228"
     CDS             66191..68512
                     /locus_tag="OO470_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6249613.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            68950..69177
                     /gene="feoA"
                     /locus_tag="OO470_000229"
     CDS             68950..69177
                     /gene="feoA"
                     /locus_tag="OO470_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6249614.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69194..71515
                     /gene="feoB"
                     /locus_tag="OO470_000230"
     CDS             69194..71515
                     /gene="feoB"
                     /locus_tag="OO470_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6249615.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71515..71751
                     /gene="feoC"
                     /locus_tag="OO470_000231"
     CDS             71515..71751
                     /gene="feoC"
                     /locus_tag="OO470_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6249616.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            71954..>72814
                     /gene="rpnA"
                     /locus_tag="OO470_000232"
     CDS             71954..>72814
                     /gene="rpnA"
                     /locus_tag="OO470_000232"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6249617.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEEL"
BASE COUNT        17549 a        19733 c        18419 g        17113 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca cgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcaa aatttgaaca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag actcaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat
     4021 aaagaagacg gccagattgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcgc ttgccggtga ggtcagttgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaacttatg gccttctgct
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg aaaatcaccg ctcttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcatcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat tcacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacatccagc agctctacgt caaacactag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag aaagcggttc accacgggtg taagagttgt cgaactcttt accgtcgatc
     5701 agcgtacctt tgtagttcac tacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct ttaagagagt tttccatgta acgacccagc
     6061 gaagcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgatgc tttgctgtct
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagtag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaagcacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ccttttcggt attagtggca
     6721 accacaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtgat gatcgtgcgc
     6841 gccgtgaacg tggccatgag ccagttcttc ttcggtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca ctacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcagtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcactag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgtgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgctataaaa cgttttcgga ttgccatcct taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc agcatgcatc gggattaact
     7741 cccgcagcat tcgcatatcc aggcggcgaa aatgcagttg cgcccgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccatat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8341 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8401 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8461 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8521 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8581 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8641 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8701 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8761 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaactcc agcaccttca
     8821 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     8881 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     8941 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9001 tcgcaaagcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9061 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9121 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9181 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9241 acgggccaat tgcaatcccc gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9301 aagagaaaca gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag
     9361 acagcggatt tgccaaccag tcaccgtagg ctctggcgtg gctcgccagc tcctttgcgc
     9421 tttgccgtct cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg
     9481 ccgccagttc aaaggggcgc agcacatcgc tcatcgggta gcgattcagc gcgtcataac
     9541 ggtaagcact ttccggctcg ccggtggtaa tcacgttacg ccagtacttt cccgccagtt
     9601 ggtttcctcc cggcccgctg gcaaaaccac gacttaatac ccggtccagc cactctttca
     9661 gtagcgccgg gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc
     9721 gcagtaatgc ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcgtaaaggt
     9781 cgtgcacggt aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg
     9841 agtcctgaga ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc
     9901 ccctcccggt atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg
     9961 gcagctcgcc catacattac attatcataa tgataagtta acatagtctg aacatacggc
    10021 gccttatgat tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata
    10081 atgccaccgc caccatcaac cccgggcaga aagtcggcct ggtgggtaaa aacggctgtg
    10141 gtaaatctac cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca
    10201 cctttccggg aagctggcaa ctggcgtggg tgaatcagga aacgccagcg ttaccgcaag
    10261 cggcgctgga atatgtcatt gacggcgacc gtgaatatcg tcaactggaa gcgcagctgc
    10321 acgacgccaa cgaacgtaac gacgggcacg ccattgcgac cattcatagc aagctggatg
    10381 ctattgacgc atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca
    10441 gcaatgaaca actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta
    10501 accttgccca ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc
    10561 acctcgatct cgatgccgtt atctggctgg aaaaatggtt gaagagctat cagggcacgc
    10621 tgatcctgat ctctcacgac cgcgacttcc tcgatccgat cgttgataaa attattcata
    10681 tcgaacaaca aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg
    10741 ccacccgtct ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtggcacatc
    10801 tgcaaagtta tatcgatcgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc
    10861 gcattaagat gctggaacgt atggagctga ttgcccccgc acacgtcgac aacccgttcc
    10921 gctttagctt ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaggtca
    10981 gcgcaggcta tggcgatcgc attattctcg actcgattaa attgaatctg gtccccggct
    11041 cgcgcattgg tctgttaggc cgcaacggcg caggtaaatc gacattaatc aaactgttag
    11101 ccggtgaact tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct
    11161 acttcgccca gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg
    11221 cacgtttagc gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt
    11281 tcctgggcga taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc
    11341 tggtgctggc attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacta
    11401 accaccttga cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg
    11461 cgctggttgt cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc
    11521 tggttcacga tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt
    11581 tgagcgacgt acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aacgcgaaca
    11641 gcgcccaggc acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc
    11701 cgctgcgtaa agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg
    11761 cgcaggcgga agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt
    11821 tgaccgcctg cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg
    11881 cgtggctgga agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat
    11941 ggcgcagata acgacgaccg atgccaatga atttagcagc agtgctgaat tcacccctat
    12001 gcgcggcttt agcaattgtc atctacaaac tatgctgccg cgtttgtttc gtcgtcaggt
    12061 gaaattcacc ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatctcgc
    12121 gtggagtgaa gaccccgcac aggcgaaaca taaaccgcgt ttagtggtgt ttcacgggct
    12181 ggaaggcagt ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg
    12241 ctggctgggc gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg
    12301 catttaccat tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga
    12361 atttggtcat gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg
    12421 tttgctggca aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc
    12481 gtttatgctg gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg
    12541 ttacttgctg aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac
    12601 gctgccgatt aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct
    12661 aatcaccgcc agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc
    12721 catgccaatg ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc
    12781 gtttatggat catcaggtga tcccaaaacc ggaaagtctc ccctcgcagg tggagtatca
    12841 actgactgaa catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat
    12901 gtggctggag tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg
    12961 attccgtggc aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta
    13021 cgtgaaggca ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc
    13081 aaacgccagc tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc
    13141 aatattatgc cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat
    13201 agtgatagct aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag
    13261 gatccagcgg cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt
    13321 taaatctgca tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa
    13381 tggacatggc gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg
    13441 aagcgaacga cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag
    13501 ggaaatctcg caaatatctg catacctacg acgaagccgt accctggaat caggtaccgg
    13561 gaacattcac cccctggcaa cctttaccag aacccactga tgtactgttt tatgaaggtt
    13621 tacacggcgg cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg
    13681 gcgtagtgcc tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc
    13741 gcgggcactc acgagaagca gtgatggact cggtagtgcg ttcaatggaa gactatatca
    13801 actacatcac accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg
    13861 acacttcaaa cccgttcgcg gcaaaaggta tcccgtcgct ggatgaaagc tttgtggtga
    13921 tccattttcg taatctggaa gggatcgatt tcccttggct gctggcgatg ttgcaaggct
    13981 cattcatttc ccacatcaat acgttagtgg tacctggcgg taaaatgggg ctggcaatgg
    14041 aattaattat gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaacttt
    14101 gctattatgc cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca
    14161 accacttcat acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa
    14221 tatttctcgg cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg
    14281 gtgacgataa aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca
    14341 gaggtcaatt ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc
    14401 acatcgatgg cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct
    14461 ttatcgcctg agttgccgtc cattaaaatc tggtgaccag aggcggattc gcccagaaaa
    14521 gttaaccctt cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt
    14581 tcctgacaga gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg
    14641 aagcgagaca ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt
    14701 aatacattga tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat
    14761 agccccttcc caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg
    14821 gctctggaga aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca
    14881 gacccgactc tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc
    14941 acgcttattc accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg
    15001 gcagtgctga tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt
    15061 gattttattg gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt
    15121 gcgaaaaccg cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag
    15181 gtaaacccgg acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact
    15241 tcagagaaag tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg
    15301 ctgaacctgg caaaacaacc agatgctatg actcacccgg acggtatgca aatcaaaatt
    15361 acccgtcagg aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg
    15421 aagatgctgg aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc
    15481 actcgttaat cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga
    15541 tgtggcgcag actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc
    15601 tatgtttgcc cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct
    15661 ccctcgttcc tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca
    15721 aacacttaat cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac
    15781 tggtaaaaga tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca
    15841 ctgctgagct ggggccattg cacgcaaaat tgcttcctgc atcgctgctc gccgccattt
    15901 ttaccctcag tctggcggga tacatgccgg tctgggaacc attgctcatc tatgcgttgg
    15961 gcactctctg gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc
    16021 tgcgcgagtc actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca
    16081 gcctgcttac ccagcacacc gatcctgaaa aagcgctgcc accgctgctg gtgcgccagc
    16141 aaaaagcggt cgatttaatt actcagtgct atcagcaaat gcatatgctt tccgcgcaaa
    16201 acaataccga ttacaagcgg atgctgcgta ttttccagga ggcgctggat ttgcaggaac
    16261 atatttcggt cagtttgcat cagccggaag aggtgcaaaa actggtcgag cgtagccatg
    16321 cggaagaagt tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg
    16381 atgacattct ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac
    16441 tggaaaaaat cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt
    16501 tcagccgcat cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg
    16561 ccgataaaca gcggcgaatg ccgttgcttc cggcactgaa aagttatctg tcactaaagt
    16621 ctccggcgct acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg
    16681 gcaccgtgct gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac
    16741 aaaatggcta tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg
    16801 ggttaatcat tgcgggcgta gcgctgcact ttaaaattcc cgaaagttac accctgacgt
    16861 tgatgctgat taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcaa
    16921 cggtcggttt tactattacc gcagtgtata ccctgcaact gttgtggctg aacggtgagc
    16981 agtacatcct tccacgtctt atcgatacca ttattggttg tttgattgct ttcggtggta
    17041 ccgtctggct gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgatgctt
    17101 tagaagccta tcaggaagcg attcgcttga ttctgagcga ggacccgcaa ccaacgccac
    17161 tggcctggca gagaatgcgg gtaaatcagg cgcataacac tctgtataac tcattgaatc
    17221 aggcgatgca ggaaccggca ttcaacagcc attatctggc agatatgaaa ctgtgggtaa
    17281 cgcacagcca gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc
    17341 gggcattgcc acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc
    17401 gttgtcagca gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg
    17461 atgcgccgga gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc
    17521 gggttattgg ccatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac
    17581 cgcatcacgg gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tacaggctaa
    17641 accatcggat aaggcattca cgccgcatcc gacatatttt gcctgatacg tcttatttac
    17701 gccccaacca ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca
    17761 tcttccacca ccagcgacgg tgctaaacgc atcacatccg gtccggcatt cagcaccatt
    17821 acgccaacct ctgcacccgc atacaggaaa tcacgcgccc gacctttgta gtgtggtttc
    17881 agctccgcgc caattaacag ccccataccg cgaatatcgc taaacacatc gtactgctga
    17941 tcgatcttct gcaaatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact
    18001 tcaggggtat tgatgatatc aaacgccgcg cccgctactg cacaggccag aggattaccg
    18061 ccgtaggtgg aaccgtgaga accaggatga aacgcagaag caatttccgc cgtggtcagc
    18121 atggcgctaa tcgggaagcc gccgcctaac gctttcgcag aagtcagaat atccggcgtc
    18181 acaccgtagt acctgtaagc aaacaaatcg ccagtccgcc ccatcccgca ctgcacttca
    18241 tcaaacacca ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct
    18301 ggcgtcgctg ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg
    18361 tgatcatcca tcaccgcttt cactgcatgg agatcgttaa agggaacgtg gatgatgtct
    18421 gccggtttcg gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac
    18481 agcgagcgac catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga
    18541 cgcacacagg cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag
    18601 ttcataaaca ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc
    18661 gccggttcat tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac
    18721 gcgttcacca gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa
    18781 tcgacatact ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaata
    18841 aactctgccg gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct
    18901 gtttgttcaa ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa
    18961 tcacaaaata tgcataaaaa atcactgaat ggcaatcgga aatcagagat gcaggaaatt
    19021 agccagcagt tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc
    19081 cagatcccac tggcgatggc gaatccccat aatctctcgg gtttcgctcc aggccgtcac
    19141 gtcaaagcac gctggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt
    19201 aagtggattt gccagccccc gaaatacgcc ctcaccgcta tgtgtaatcg gcgaggtttt
    19261 gccgtgcatg acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg
    19321 atgaccgagg cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag
    19381 ggagatcccg gcttcatctg gcgtacaggg gccaggtgag atgacaattt tttgtggttt
    19441 aagggcgtcg atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc
    19501 cagttcacaa aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag
    19561 gatcatggcg gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc
    19621 ttaccacttt gctaaatatc atctgcagtg cggtgagatc ccccattgcc ccactctgat
    19681 ttgcctgatt ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa
    19741 tcgccagttg ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca
    19801 gcacgttgat ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc
    19861 caaccagata accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat
    19921 aagcaaagtg gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg
    19981 cccagtcgaa aatatcctgg tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc
    20041 gcaccagcgg accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct
    20101 gttccagccg ctgctgctgg cggatgttca gccggttacg catgatatca aggccaggat
    20161 aaagatacgg atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca
    20221 aggcgcgcta aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga
    20281 cggcgactgg cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta
    20341 tcggtgagtt tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg
    20401 gcaaatccgg gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc
    20461 attacggcag gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg
    20521 gaccaacatc atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca
    20581 ctttaccaaa taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg
    20641 caacgttgat aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga
    20701 tggtgccacg cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt
    20761 ttttctgctg catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc
    20821 ggtgaaaggt agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag
    20881 acactggcgc tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca
    20941 atacgtgcgg gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcagaaa
    21001 gagcgaaaac agcagccatc gccgccaggg tcgatttgaa catgagattt ccttaacaaa
    21061 cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc ttcaagaaca aggccatccc
    21121 tttacctaat tttacgtatc tgaaatatat gtaacctgac gatcactttt tattccgtga
    21181 tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc aataaaacat ttaaacagat
    21241 cataaaatca cctaaaatcg cccgctcgcg gcatatcccg ctatgccatt ttatttttga
    21301 acgcaggcca atcatgacta acagcaatcg catcaagctc acatggatta gctttctctc
    21361 ctacgcactg actggtgcgt tggttattgt caccgggatg gtgatgggaa atatcgccga
    21421 ttatttcaat ctgcctgttt ccagtatgag taacaccttc accttcctca acgccggcat
    21481 tttaatctct atcttcctca acgcctggct gatggaaatc gtcccgttga aaacgcagtt
    21541 acgttttggc tttctcctga tggtgctggc ggttgccggt ttgatgttca gccacagcct
    21601 ggcgctgttc tcgacggcga tgttcattct cggggtggtc agcggcatta ctatgtcgat
    21661 tggtacattc ctgataacac aaatgtatga agggcgtcag cgcggttccc gcctgttatt
    21721 tactgactcc ttcttcagta tggctgggat gattttccca atgatcgccg cgtttctact
    21781 ggcgcgcagc attgagtggt actgggttta tgcctgcatc gggctggtgt atgtcgctat
    21841 ttttattctg accttcggct gtgagttccc ggcactgggc aaacgtgcac caaaaacgga
    21901 tgctccggta gagaaagaaa agtgggggat cggcgtactg tttctctctg ttgcggcgct
    21961 gtgctacatc ctcggtcagt taggttttat ctcctgggtg cctgagtatg ccaaaggcct
    22021 gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac ttctggatgt catacatggt
    22081 cggcatgtgg gcgttcagct ttattcttcg cttctttgat ttgcaacgca ttctgaccgt
    22141 actggctggt ctggctgcga ttctgatgta cgtctttaac accggaacac cagtacatat
    22201 ggcgtggtca attctcgctc tgggcttctt ctccagcgcg atctatacca ccatcatcac
    22261 cctgggttca cagcagacca aagtaccgtc gccaaaactg gttaactttg tcctgacctg
    22321 cggaaccatc ggtactatgt tgacctttgt ggttaccggc ccgattgttg aacatagcgg
    22381 tccgcaggcg gcactgctta cagcaaacgg tctgtacgct gtcgtctttg tgatgtgctt
    22441 cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc attaattgct
    22501 aatgccggac ggcactatcg tcgtccggcc ttttcctctc ttcccccgct acgtgcatct
    22561 atttctataa acccgttcat tttgtccatt ttttgcacaa acatgaaatg tcagacaatt
    22621 ccgtgactta agaaaattta tacaaatcag caatataccc cttaaggagt atataaaggt
    22681 gaatttgatt tacatcaata agcggggttg ctgaatcgtt aaggtaggcg gtaatagaaa
    22741 agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg gtatggtcgg
    22801 ccatcgcttt atcgaagatc ttcttgataa atctgatgcg gccaactttg atattaccgt
    22861 tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac ctctcgtctt acttctctca
    22921 ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac acggcatcaa
    22981 agttctggtc ggcgaacgcg ctatcaccat caaccgtcag gagaaggtga ttcactccag
    23041 cgccggacgt accgtttttt atgacaagct gatcatggca accggttcct acccgtggat
    23101 cccaccaatc aaaggttctg atactcagga ctgctttgtc tatcgcacca ttgaagacct
    23161 caacgccatt gaatcctgcg cccgtcgcag caaacgcggt gccgttgttg gtggcggcct
    23221 gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt attgaaaccc acgttatcga
    23281 atttgcccct atgctgatgg cggaacagct tgatcagatg ggtggcgagc agctgcgtcg
    23341 caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa aacacccttg agattgtgca
    23401 ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac tggaagtcga
    23461 ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc agtgtggtct
    23521 ggacgttgct ccgcgtgggg ggattgtcat taatgattcc tgccagactt ccgatccgga
    23581 tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc tggtagcacc
    23641 aggctacaaa atggcgcagg tcgccgttga ccatattctg ggtagcgaaa acgcctttga
    23701 aggtgctgac cttagcgcca agctgaaact gctgggcgta gacgtaggtg gtattggtga
    23761 tgcgcacggt cgcacgcctg gcgcacgtag ctatgtttac ctcgacgaaa gtaaagagat
    23821 ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg ctcggtgcgg tactggtggg
    23881 cgataccagc gactacggca acctgctgca actggtgctg aacgctatcg agctgccgga
    23941 aaacccggat tctctgattc tgccggcgca ctcgggtagc ggtaagccgt ctatcggtgt
    24001 tgataaactg ccggacagcg cgcaaatctg ctcctgcttc gacgtcacta aaggtgatct
    24061 gattgctgcc atcaacaaag gctgccacac agttgcggcg ctgaaagctg aaaccaaagc
    24121 gggtactggc tgcggtggct gtatcccgct ggtcactcag gtactgaacg cggaactggc
    24181 gaaacagggc atcgaagtta acaacaacct gtgcgaacac tttgcttatt cgcgtcagga
    24241 actgttccat ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc tggcgaaaca
    24301 cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc ggttcgctgc tggcctcctg
    24361 ctggaacgaa tacattctga agccggaaca tactccgctg caggattcta acgacaactt
    24421 cctcgctaac atccagaaag acggcaccta ctcggtgatc ccgcgttctc cgggcggtga
    24481 aatcaccccg gaagggctga tggcagtggg tcgtatcgcg cgtgaattta atctctacac
    24541 caaaatcacc ggctcccagc gcctggcgat gtttggcgca cagaaagacg atctgccgga
    24601 aatctggcgt cagctgattg aagctggctt cgaaaccggt catgcctatg cgaaagcact
    24661 gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc tacggtgttg gcgatagcgt
    24721 cggcctcggc gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc acaaaatgaa
    24781 gttcggtgtc tccggctgta cccgtgaatg ttcagaagct cagggtaaag acgtgggtat
    24841 tatcgccact gaaaaaggct ggaacctgta tgtttgcggt aacggcggca tgaaaccgcg
    24901 tcatgcggat ctgctggcag cggatatcga tcgcgaaacg ctgatcaaat atctcgaccg
    24961 cttcatgatg ttctacatcc gtaccgccga caaactgacg cgtaccgcgc cgtggttaga
    25021 aaacctcgaa ggcggcatcg attacctgaa agcggtgatc attgacgaca aactggggct
    25081 gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt gtgagtggac
    25141 tgaaacggtc aatacaccgt ccgcgcagac tcgcttcaaa cacttcatca acagcgacaa
    25201 gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg caacgccgta
    25261 tgaacgtatc ccggtaactc tggtggagga caacgcatga gccagtggaa agacatctgc
    25321 aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcac tgttaggtga caagcaggta
    25381 gcgattttcc gcccgtatca cagcgaccag gtgtttgcga tcagcaacat tgatccgttc
    25441 ttcgagtcca gcgtgctatc acgcgggctg attgcggaac accagggcga gctgtgggtc
    25501 gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg gcttgtgcat ggaagacgaa
    25561 cagttttccg tcaaacatta cgaagcacga gtgaaagacg gcgtggtgca gctgcgcggt
    25621 taatgtttta acgggaggcg caatgcctcc cctttttgca tggtcctgta atgaccttcg
    25681 gtatattgca ggacattttt taaacttttt gttttatttt ttgtttttat tttttaaagg
    25741 ataatcaaat gtttacagac actattaata agtgtgcggc taatgctgcg cgcattgcac
    25801 gcctgtcggc aaataacccg ctcggctttt gggtcagctc cgccatggcg ggcgcatatg
    25861 tgggtcttgg gatcatcctg attttcacgc tcggtaattt gctcgacccg tccgtacgtc
    25921 cgctggtgat gggcgcgacc tttggtatcg ccttaacgct ggtgattatc gccggttctg
    25981 aactgttcac cggacacacc atgttcctca cctttggggt aaaagcgggc agcatcagcc
    26041 acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg taacctggtc ggttccgtct
    26101 tcgttgccat gctctatagc tggggcggcg gtagcctgct gccggtagat accagcatcg
    26161 ttcactccgt cgcgctggct aaaaccactg caccggcaat ggtactgttc ttcaaaggtg
    26221 cattgtgtaa ctggctggtt tgcctagcaa tctggatggc gctgcgcact gaaggggcgg
    26281 cgaaatttat cgctatctgg tggtgtctgc tggcatttat cgcgtccggc tacgagcact
    26341 ctatcgctaa catgacgctg ttcgcgctct cctggttcgg caaccacagc gaagcctaca
    26401 cgctggcggg tattggtcat aacctgctgt gggtgacgct gggtaatact ttatcaggtg
    26461 ccgtattcat gggattgggt tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg
    26521 acaaatttaa tcaaactgaa acagctgccg gttaattact aaggggtttt tacgtggatc
    26581 atttgcctat attttgccaa ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg
    26641 tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat
    26701 tagcgtttat tccacagttc accgcatggg cagatgcagg catgttaacc ctcgtcgaag
    26761 ggccatttga tgaaagcctt ctcgacacct gctggctggc gattgcagcg acggatgatg
    26821 acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtag
    26881 ttgatgcgcc gaaagccgcc agctttatta tgccgtcgat tattgaccgc tcaccgctaa
    26941 tggtagcggt ctcctctggc ggcacctctc cggttctggc gcgcctgttg cgcgaaaaac
    27001 tcgaatcact gctgccgttg catctgggcc aggtggcgaa atatgccggg caattacgcg
    27061 ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg ccgtttctgg gagaaattgt
    27121 tcgttaacga ccgcctggcg cagtcgctgg caaacaacga tcagaaagcc attactgaaa
    27181 cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg tgaagtggtg ctggttggtg
    27241 caggtccggg cgatgccggg ctgctgacgc tgaaaggact gcaacaaatt cagcaggcag
    27301 atgtggtggt ctacgaccgt ctggtttctg acgatattat gaatctggta cgccgcgatg
    27361 ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg tgtaccgcag gaagagatta
    27421 accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt ggtgcggctg aaaggtggcg
    27481 atccgtttat ttttggccgt ggtggcgaag agctggaaac actgtgcaac gcgggtattc
    27541 cgttctcggt ggttccgggt attaccgcag cttctggttg ctctgcctat tcgggtattc
    27601 cactcacgca tcgcgattat gcccagagcg tacgcttaat taccggacac ttaaaaaccg
    27661 gtggcgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg ttctatatgg
    27721 ggttgaatca ggccgcgact attcagcaaa agctgattga acacggtatg cctggcgaaa
    27781 tgccggtggc aattgtcgaa aacggaacgg cagtcacgca gcgcgtgatt gacggtacgc
    27841 tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc atcgctaatt attattggtc
    27901 gggttgttgg cctgcgcgat aaactgaact ggttctccaa tcattaattt aacccgggcc
    27961 agagaattct ggccttctta atgactcttt ttatatattc agcaaataaa acataacccc
    28021 ttaataaata ttatctgcta atgggttata ttcaccgtta cagcgactta caaaattaag
    28081 tcattcgctc actgacgcca attaaatatt cctgtatcgt tcttaacaag cagtctgctt
    28141 atattcatat aatcaatgaa tattaattaa tatataatac atagggaatg taaatgaaca
    28201 aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca tgtactggtc
    28261 atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta
    28321 tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc
    28381 taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctatg
    28441 tccttcttgc ccccgcccgt aaaaatattt tcactttaaa ttcaatttta taactacatc
    28501 cattagccac agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat tacataacat
    28561 aatatgttgt tatattcatc atgcattgtc atgttacctt ttaaatgact gcaaactctc
    28621 ccctacaacg tattggacaa gaaaaaggta tcgctatggg aagccaagaa ctccaacgca
    28681 agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac aaccgtcggc tccggtattt
    28741 ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc gtggcttacg gtgctcgcgt
    28801 ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg
    28861 cttatccgga aaatggcgca gattatgttt atctgaaaaa tgccggaagc cgaccgctgg
    28921 ctttcctctc cggctgggcc agcttctggg ccaacgatgc gccgtcattg tcgattatgg
    28981 cgctggcgat tgtcagcaat cttggctttt taacgcctat cgatccgttg ctcggtaaat
    29041 ttatcgccgc cggattaatt atcgccttta tgttgctaca cctgcgctcc gttgaaggcg
    29101 gcgcagcgtt tcagacgcta attaccatcg ccaaaattat cccgttcact atcgtcattg
    29161 gccttgggat cttctggttt aaagcggaga attttgccgc ccttaccacc actgcgattg
    29221 gcgcaacggg cagctttatg gcgctgctgg cggggatctc tgccaccagt tggtcgtata
    29281 ccggcatggc ctctatctgt tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc
    29341 cacgagcgct gattggttcc tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg
    29401 tgatttccgg cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg atttccgacg
    29461 ccctgaccta gatccccgca ctcggcagca ccgctgggct ctttgttgcc atcacggcga
    29521 tgatcgtcat tcttggttcg ctttccagct gcgtgatgta ccagccgcgg ctggaatacg
    29581 cgatggcgaa agacaacctg ttctttaaat gcttcggcca tgtgcatccg aaatacaaca
    29641 cgccggatgt ctccatcatc ctgcaagggg cgctggggat cttcttcatc ttcgtttccg
    29701 atctcaccag cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat accctcacct
    29761 tcggctccat catctggtgt cgtaaacgcg acgattacaa accgctgtgg cgtactccgg
    29821 ctttcgggct gatgaccacc ctcgccattg cgtcaagcct cattctggtc gcctcaacct
    29881 ttgtctgggc accgattccc ggccttatct gcgccgtcat cgttattgct actggtctgc
    29941 ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg taatgttatc
    30001 tgaagaaaat aaaatgttgg atattgataa aagcaccgtg gactttctgg tcaccgaaaa
    30061 tatggttcag gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc acgccatcgt
    30121 ggaagagatg gtgaagcgcg acattgatcg tatttatttc gttgcctgcg gctcgccact
    30181 caacgcggcg caaacggcga aacatctggc ggatcgcttt tccgatcttc aggtctacgc
    30241 catttccggc tgggagttct gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt
    30301 gattggcgtt tctgactacg gtaaaacgga agaggtaatc aaagcgctgg agctgggccg
    30361 ggcctgcggc gcactcactg cggcgttcac caaacgcgcg gacagcccga ttacctcggc
    30421 ggcggaattt agcattgatt atcaggccga ctgtatctgg gaaattcacc tgctgctctg
    30481 ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat
    30541 caaaaacgat ctcaagcagt tgccgaatgc gctcggtcat ctggtacgca cctgggaaga
    30601 aaaaggccgc cagcttggtg aactggccag ccagtggccg atgatttata ccgttgctgc
    30661 gggtccgctg cgtccgctgg gttacaaaga aggcattgta acgctgatgg aatttacctg
    30721 gacgcacggc tgcgtgattg agagcggaga gttccgccat ggcccgctgg agattgtcga
    30781 accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg
    30841 cgccattaac tttgttaaac agcgtactga caacgtgatc gtcatcgatt acgccgaaat
    30901 ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa tggagtggct
    30961 ctgctactac ctgtctattt acaaagatca caacccggat gaacgccgct attacggtgg
    31021 tctggtggaa tattaatccc tctcccggcc cggcaacggg ccggatttac gcaaggagtt
    31081 acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat
    31141 ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg
    31201 cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt
    31261 atcagatgcc gattatcggt tacacgccag aaaccaacgg ctatccgtat aacatgatgc
    31321 tgggcgatga acatatgcgt cgtgaaagcc tggacatgat caagctggcg atagatatgg
    31381 caaaagagat gaacgcgggt tatacgctga tttctgcggc ccacgcgggc tatctcacgc
    31441 cgcctaacgt tatctggggg cggctggcag agaacttaag cgaactgtgt gagtacgcgg
    31501 aaaacatcgg catggatctg atcctcgaac cgttaacgcc gtatgaatcg aacgtcgtgt
    31561 gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca
    31621 tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt gatgagttat ttcgacaaac
    31681 tgggcgataa attacgtcat ctgcatattg tcgacagcga cggggccagc gacacgcatt
    31741 acattcctgg agaaggcaaa atgccgctgc gggaactgat gcgcgatatt attgatcggg
    31801 gctatgaggg ttactgtacg gtggagctgg tgacgatgta tatgaacgag cccagactct
    31861 atgcccgcca ggcgctggaa cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac
    31921 cctggcgaca atcggcgata actgcgtcga tatctacccg caactgaata aagcgttttc
    31981 tggcggtaat gcggtcaatg tggcggtgta ctgcactcgc tacggcatac agccgggatg
    32041 cattacctgg gtgggtgacg atgactacgg cacaaagctg aagcaggatc tcgcccgcat
    32101 gggcgtcgat atcagccatg tccatacgaa acacggcgtt accgcacaaa ctcaggtgga
    32161 actgcacgac aatgatcgcg tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc
    32221 cctgagtgaa gaggattacg cctggctggc gcagtatgac attgtgcacg cggcaatctg
    32281 gggacatgcg gaagacgcat tcccacagct gcacgctgcg ggcaaactta ccgctttcga
    32341 cttctccgac aagtgggaca gcccgctctg gcagacactg gtgccgcatc tcgattttgc
    32401 ctttgcctcc gcaccgcaag aagacgaagc gctgcgtctg aagatgaaag cgattgttgc
    32461 ccgtggcgca ggaacagtga ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg
    32521 cgcgcagttc tggcgtcagg ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg
    32581 agattcgttc attgccggat tcctttgcgg ctggtctgcg gggatgacat taccgcaggc
    32641 gatggcgcag ggaacggcgt gcgcggcgaa aaccattcag taccacggtg cctggtaggt
    32701 ataacgttgg cgtgagcatc ttcacgccaa cgtgctgtta cttgccggaa aacgacccta
    32761 taatccgagt aattcattct ttatttcagg gtcgattatg tcagctacgg accgctactc
    32821 tcatcaactc ctctacgcta ccgtccgcca gcggctgctg gatgatatcg cgcagggggt
    32881 ttaccaggcc gggcaacaga tccctaccga aaacgagctt tgtacacaat ataacgtcag
    32941 ccgcattacc attcgcaaag ccatcagcga cttagtggca gacggcgtac tgatccgctg
    33001 gcagggaaaa ggcacctttg tacaaagcca gaaagttgaa aacgccctgc ttactgtcag
    33061 tggttttacc gattttggcg tctcacaagg caagtcgacg aaagagaaag tgatcgaaca
    33121 ggaacgggtc agcgccgcgc cgttttgcga aaagctgaac atccccggaa acagcgaagt
    33181 gttccatctc tgccgggtga tgtatctcga taaagagccg ctgtttattg atagttcatg
    33241 gatcccgctg tcgcgttatc ctgactttga tgagatttac gtcgaaggaa gctccaccta
    33301 tcagttattt caggagcgtt ttgacacgcg agtggtcagc gacaaaaaga ccatcgatat
    33361 ctttgccgcc acccgcccgc aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt
    33421 tcgcatcagc aaaatcgcct ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt
    33481 ctgccgcgcc aatcgcatca ccttaacgat tgataataaa agacattaac cgtaggccgg
    33541 ataagatgcg ccagcatcgc atccggcgat gctggcgcac tgaattttac atcccgcacg
    33601 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcagtg cggaatttag
    33661 cttgattgcc gaacggtgct atccagcatt aaatactcgc aatactctca cgcaaaatcc
    33721 gcagcaccgt ctcttcaccg ctgcgattcg ggttaatgcg aatcgcacaa tgttcgagtt
    33781 gtggattcgc ctggcgaaac gttcctgaaa gtcgatagaa gagcggcggg atttcatatt
    33841 tcgactctgc accgaccggg taaggcaacg cgccacgctt ttgcgcttct tccagcaccc
    33901 tggcggcaat cagctgatga aactcgacaa tcaacacctt cgactgcgca ttggcaatga
    33961 cggcgctttt cacttccgcc accgcgccgc cgttaagcaa agccagcaac cgttcggata
    34021 ctcccgcctg caccgcatgc atcactggcg cgaacaccag accacgcaag acttccagcg
    34081 cctgtgagcc ctggatctgg ctaccgccgg agtaaagcgt ggcgcgaatc cgactaataa
    34141 catcggcatc gccaaccact gcaccaacgc cccccggccc aaacagcttg aagcaggaga
    34201 atgtcgagac attcgcgccg cattcacagc cgattcgcgc taccttcatc accgcatagt
    34261 tatcatcggt taacgctgga acacctgccg cgcgcaacgt tgccagcaca tctgccagca
    34321 catagctgtc ctgcggctgc tggcgcgtat gctgcaccag cgcggcatca ggttgttgct
    34381 cgtcgacgac ctgcttcagt gccgacaggt cattgaaatc agcagtaata agcgtcagcc
    34441 ccatctgctc aataataacc cgtgtcgtcg ggtaaacagg cgcgtcatgc accagaagac
    34501 gctgccccgg tttgagcaaa gccgccagcc cggcgcgaat cgcgccagtc cccgcgccct
    34561 gcaccagcgc cgcggcctgt gcgtgaaatg catcagccag cacctgctcc acccgttggg
    34621 taatacgcgg ttgattcaga cttggagtta accctaaatc accgccagca agaaactcgc
    34681 tgcctggaaa atggcggcaa atagtgtcca ccagcgcaaa ctgcttttgc tgcgcttcag
    34741 caagagtcag gctttgcaaa ggaaacgtct tcataggtgc cttccttaag ccggaacgaa
    34801 caggccaagc cagtaaagaa cattcagcag aataccggtg atcattactg ccacgaccgg
    34861 tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc
    34921 gatagagaat ccggtataac ccgccatctt aattgctgcg aaaatcgaac cgaccagcag
    34981 cgccacttcc atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgcaccga
    35041 cgggtagcgt cccagccatt tgccgatcga acgaagcagc aagacttccg ccgaaataac
    35101 cactgcgcct aataccgctg caaccatcgg attcggcgag agatagccca ccgcataaac
    35161 aaaggtaaag cccgcaactg catacacacc cgttgctaag gcggtggtgg caatcaacgg
    35221 cacaaacccc agtccgcgca taaattctgc cagagccgcc tgattaatca gcgtttgcga
    35281 ttgttccggc gtcacgcctg cggaatacgc tttctccagt gtgaagatcg aaacttcgct
    35341 accagcaaaa atcttcatac tggcaacggc ggcaatcaat gctcccacga tggcgatata
    35401 aggtaagttt ttgataatcc gcgacgtgcg ctcttcaaac accgaaagcc cgctggcatc
    35461 gatgtcattt tcatcacgat gacgcaggtc gtgagttatc gcgatcccca gcagcatcac
    35521 catgccaata aagatttcga tggattcagg gttaagatgt gggaaatagc gcacgactac
    35581 cacacgggtc atcagaacaa ccacggcggc gaccagactt tgcttccagc caaactggta
    35641 gaaaatcgcc accagcggga acagtgcaaa agcagaaacc accggcgagc ttaattcccc
    35701 gaggctacct aatacatcca ccggcagcgc ggtcagcagc tggttaactg gcaacaggca
    35761 agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc
    35821 cagtacgccg aggatatcgg taggaagaaa gagtaaccat gcgttgagca gcccggtttt
    35881 cagggtaaaa gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact
    35941 ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg ggtggatgcc
    36001 gtcatgaaaa acagccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc
    36061 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
    36121 aacatcggaa tcgcgtgttc gacgtgctca acggaaaggc caaacgccac tttcccttca
    36181 gcgatcattt tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa
    36241 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
    36301 cacgcgccaa tgtaataatc tagttgcccg gatttcacct tcattgccgc ttccatgtcg
    36361 ttgtgaatga aaacttcaaa acagccagga gccgtcgctt caatggtttt tttaatctgc
    36421 tcacgctgta agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg
    36481 agagggattt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
    36541 ttgcggaata aaggtggtca ataaatagtc atagccataa ccaccattgg cttttaaatg
    36601 ggagcggcgc gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcacgtag
    36661 cgcgtggagc atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttac caatggtgtc
    36721 gaactgcacg tacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt tgtctttcag
    36781 atcgcagtga ccaacggtga cgcgcgaaag atcaactccg tgggcttgca acagagccag
    36841 ttgttccagc cccatggtgc tgaacgacgt atgcgtggag atcgggcgtc cggtctggtt
    36901 atgcgccagc gcagcggcaa taaatacctt ctcttccagc ggcgtaatct ttccttcgct
    36961 ggagccgatc tccgcgatga tcccggcttt cagctccgtg ccatcgatac cctgttcaat
    37021 ttcatcgacc atctcctgcg ccagttcctg cacgctgcgg gtcaccacat gttccgggaa
    37081 aaacgcatcc tggtaataac cagtacaggc caccacgttg atcccggttt cgcgcattac
    37141 atcgagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
    37201 tacaccccgg gtcatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg
    37261 gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat gggccagggt
    37321 gtaacccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
    37381 gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac acacggtagc tcccacatca
    37441 gaaagcgtgg tgcgcacacc gagctgcgtg gcgaccagcc cttgctgata aaccagcact
    37501 ggcaccactt cacgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc
    37561 atcacgacca ggcaatcatc tggctgcatc gcctcaacaa gccgggcaag gttacggtca
    37621 acgacctgca aacgttcggc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt
    37681 tcctggatat tgatgcaaat aaacgccgtc ggatgggtgt taaattcgtc gagggtgata
    37741 tccataatcc gctggctatc caccaggttt tgccagctca caccataagg attgctgacg
    37801 atatctgcca ccttacccac cagcacggtt ggcacgcccg cttcatacag tttttgcggc
    37861 acctgcactt tttcatcaac accgtagccc atatgcacga cctggaagcc gttgtcataa
    37921 gcgccagaac gcggcgcatt aataccaata aagcgccctt ctttgctttc tgcggcatcg
    37981 agaatacgct gactgttggt taacaggccg ccaaatgtaa tgacccgacc gacctgtacc
    38041 tggtcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac agagagattg
    38101 gcggtaatgt tatagacttg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
    38161 tgattgaccg acagaaattg cagatcatcg ccacggcgct ccacctgcca gccagcggaa
    38221 actaatgcct gctcaacacg gtcaatcaca tcgcgaaaag gcatccgcag cggcggtaac
    38281 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
    38341 tctgccacgc cccaggttgc ggaatctgac ggctgcatat cgcctggcgc ataacccaat
    38401 gtgttgatta gccccagctt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg
    38461 atgtgaccac aggtattcgc cccggcatct tgcgggcgca ccagcgtgac atctttcatt
    38521 gcccctacgc caaagctatc aatcactaac accacaaatc gcgccattta tgcctccaga
    38581 gggttgccga ggctgtcata acgaccaact atttctggtt cgccgcgctg aataccggac
    38641 accagcacca catcgctacg ggtgacaaaa atctgcgtgc gaaaacagag caccactgca
    38701 ctgctcaccg gatactcgcc tgccagcggc agggtgtagt cgatactgct gtcatccaca
    38761 gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag cgcatgttgc
    38821 gcatgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg gaaatgatgg
    38881 gagatttcac ttaaccagag catcgcgata cgttcaggct gatcgccctg ctggtttgcc
    38941 ggaatagtac cggtcagcgc atgaccgggt tcggcatgag tcgcaccgta ttccgccagc
    39001 aatggcagcg aagtgcagct ggtcgctgaa ggtacgttca gttgctcaat cgcaatacca
    39061 gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt tggcaaaact
    39121 ttcccagcag cctcatccca aagcaggcaa gggaaatggg ttagtccggc taaatgcagc
    39181 cccggcagtt tcttaatttc agcaatcacc tcgtgcagcg aatgctgtac aaaaccgctc
    39241 tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac aggctggacc
    39301 cgtccggcct taaccgctgc cgcagaaact tcccgcgctt tgtcgagagt aaacacggtg
    39361 atgatgtcgg tgccctgttc aacggcgtca gcaacctgat gacaagggat ttgtaccaga
    39421 tgcccctgat gcgccactgg caaaccagcg cggcgcatga ctcgcgcctc tttgtaatcc
    39481 accgccacaa tgccgctgta gcctaatgcc agcaattttt ccgccagcca cggattacga
    39541 ccaaattgct tggtcatcag atacagttca atgccgtaaa gccgcgccgt ctcaatgagc
    39601 cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg ggcgattttg
    39661 ccctgctgcc acaaacttag tgcggcggaa atcagcgccg ggttctggcg tttcaatgct
    39721 tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttttgaat
    39781 atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta gcagccatcc
    39841 ataatccata aagattggcc agaaaatagc cttcttcaca cgggttaact tccagagcga
    39901 attccttcaa caactcctga tgcaattgca caacggcttg ccagtggctg gattgcgcca
    39961 gttccgccag caactcgtta tccagcggct ctatttcttc accacggcga ctgcgcatca
    40021 gtgcactcgc catatgtgtc atcgccatcg ttccttgctc actgcgcacc ggcagatggc
    40081 actctttttc cagtacgttg acgacctgca tcatgccttt gcagacgtcc ttatcaataa
    40141 cgcctgcctc gcaaagcagg ttgagtctgg tttccatatc agatgccttt cgtttaataa
    40201 ctgggttcct gttccccact caccacccgt tgttgatggg caagcagggc gtgtttatct
    40261 acaacagcac gcagcaattg ttgcattggg taatcatcga ctcgcgtcag gaccacggct
    40321 tcggtggcct gtaaaaatcg cggatcgtct gttagcggcg tcgcctctaa tcccagcatg
    40381 gtcagttcgt tttccgccac cacgttccag atcaccgcat cgacatcgcc tttaacaatg
    40441 cgttgtaaac tctcgtgata agagagatcg actcgttcca catcactatc gccaaaaaag
    40501 acatcggtca tgattttctg atccgccgaa cgactatcga gacctactcg cttaacattt
    40561 ccagactcac ctttgcggca tatcagttga tgttcaccaa catacgtatg cgggccaagc
    40621 tcgagggcga tgcacaaatt gttttggctg agataacttt ccgccgccag gcgggaaact
    40681 actgccatgt catagacacc gttaagtaaa cactccacgc gaatatccgc gccgcgcata
    40741 tgcgcatagt aaaaaggaat gccatcaaac tgggctttca atccgctcgc taaaccttcg
    40801 tacaagcggg tatagggcaa aggcatcgca cataccacgt tgttgatatc cacatgactc
    40861 agcaatgctt tgttatccat ctcgacaaga taactgccat tgcgcccacg gcgttcaatc
    40921 cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aaccgatgaa
    40981 cggcattcag ttgccagctc atctatggtt ttcagtcgat taccgcattt ttcacccaac
    41041 aaataacggg ccagtgtcgt gatgacgacg ccttcttttt tgataaacgt tcgacgcata
    41101 atatattttc aataaaatga atatttatat cttcattaaa atgaagataa gtggcgacga
    41161 cgagatgtgg aaacggcgag gcgcttcaca ttttttcggg ttgttgcgaa aagaggataa
    41221 aaaaaccggg tttccccggt ttcagagtga tgacgaagtg atttattcga caatgcgagc
    41281 aaagggtaat tcccactggc gcttgcctgc tcccctcacc ctaaccctct ccccaaaggg
    41341 gcgaggggac cgtccgagcg ttttgatatt acggcttcgc cacaaaacca atcgcttcgt
    41401 acaccgcttt cagcgtacgg gaagcgtgta cactggcttt ttctgcgccg tctttcatca
    41461 cctgttgcag gaaggcttca tcgttgcgga aacggtgata gcgttcctgc aattcagtca
    41521 gcataccgga aacggcatca gccacttcac ctttcagatg accatacatc ttgccttcga
    41581 actgtttttc cagttctggg atgctctggc ccgttaccgc agaaaggata tccagcaggt
    41641 tggaaacgcc cgctttgttc tgcacatcgt agcgaactac cggcggctcg tcggagtcag
    41701 tgaccgcgcg tttgattttc ttcactaccg atttcggatc ttccagcagg ccgataacgt
    41761 tattgcggtt atcgtcagac ttggacatct tcttggtcgg ctccagcagc gacattacgc
    41821 gcgcgccaga tttcggaata aacggctccg gcaccttaaa gatctcgcca tacagcgcgt
    41881 tgaaacgctg ggcgatatcg cgactcagtt ccaggtgctg tttctggtct tcacccaccg
    41941 gtaccagatt agtttgatac agcaggatgt ccgcagccat cagcaccgga tagccaaaca
    42001 gaccagcgtt gatgttctcg gcataacgcg cagatttatc tttaaactgc gtcatgcgac
    42061 tcagttcgcc gaagtaggta tagcagttca gtgcccagcc taactgcgca tgttctggta
    42121 cgtgggactg aacaaaaatg gtgcttttct caggatcgat accacaagcc agatacaagg
    42181 ccagcgtatc caacgtcgct ttacgcagct tctgtgcatc ctggcgcacg gtgatcgcgt
    42241 gttggtcaac gatacagtaa acgcaatggt agtcatcctg catgtttacc cactgacgca
    42301 gcgcacccat gtagttacca atggtcaatt cacctgaggg ctgtgcgcca ctaaaaacga
    42361 tgggcttagt catttttcga ttcctgattt tcgctatgcg gaagccctaa tgcgggcaga
    42421 aggtcattta tagactgata aattacatca ggctggctga gatcgatagc ctcgccgtag
    42481 ttgtatccgt aggttaagcc aactgatggg caacctgccg cttttgccgc ctgaatatca
    42541 ttccgtgagt cgccgacaaa cagcatctgt tgtggggcaa ttcccatccg ctcagccacc
    42601 agtaacagcg ggtccggatg cggttttttg ttttgcacat catcgccgcc gataaccacg
    42661 ctaaagtatt tggcaatatc taaggcttcg agcagcggcg cgacgaacgg cgtcggtttg
    42721 ttggtgacca agcctagcgg caggccttta gcctgcaacg cgcccaacgt atcggcaacg
    42781 tgcgggaaca aaaacgtccc ctcttcggca acctcgccat agtagcgatc gaacagttta
    42841 cgcagaatac gtacctgttc ttctgccgga atgtcgtcat caacgggcgg tttccccatt
    42901 gttttacgca gagtcgcacg ttcctgacgc gcccaggtca atgcgcgctc catcagaaca
    42961 tctgcgccgt taccaatcca ggtaataacg cgttcttcac ctgcgacggg caactccagc
    43021 gcatacagcg ccatatctac cgcagcagca agaccaggag cactgtcgac cagcgtacca
    43081 tcaagatcaa aagcgacgcc gcgaatatct tcaaacttat tcatgactta cctttgccag
    43141 ttcactgcgc atttcatcaa tgactttttt atagtctggc tggtcgaaga ttgccgaacc
    43201 ggcgacgaac atatccgcgc ccgccgcagc gatttcgcca atgttgttca ccttcacgcc
    43261 accgtccact tccagacgaa tgtcaaagcc agactcgtcg atacggcgac gtacttcgcg
    43321 cagtttatcc agtgtttgag gaatgaaaga ctgaccgccg aaaccagggt tgacggacat
    43381 cagcaggatc acatccagct tatccatcac gtaatccaga tagctcagag gtgtcgccgg
    43441 gttaaatacc agacccgctt tacagccatt ttctttaatc agttgcagcg tgcggtcaac
    43501 atgctcggag gcttctggat gaaaggtaat gatgctggca ccagcggcag cgaaatcagg
    43561 cacaatgcga tcgacgggtt tcaccatcag atgtacgtcg ataggggcgg taatgccgta
    43621 gttacgcaag gatttcagca ccattggccc aatcgtcaga ttgggaacat agtggttatc
    43681 catgacgtca aaatgcacga catcagcgcc agctgccagg gcttttgcgg tatcttcacc
    43741 caggcgggca aaatcagccg acagaattga gggggcaatc aaatactgtt tcatccgctt
    43801 ctccttgaga attatttttt cgcgggtgaa acgactcctg gtttgtacaa agccagcagt
    43861 tcgtccacct ttttacgtgt gccgccgttg ctgcttatac tgcgtcgaac tttgacgaca
    43921 tgcaattttg cgcgctgata ccactcacgc gttaacatcg tatcgtgatt ggagatcagc
    43981 actggaatat ggcgctcaac cagaccttcg gcgatctccg ccagatgcgc ttgttgttca
    44041 agcgtaaaac tgtttgtgtg atacgccgta aagttggcgg tcgcagacag cggtgcataa
    44101 ggcggatcgc aatagacgac ggatgcatca tctgcgcgct ccatgctatc ggcgtaagac
    44161 tcacaataga aaaaggcatt ctgcgctttt tcagcgaagt gatacaactc tgcttccggg
    44221 aaatagggtt ttttgtagcg gccaaacggc acgttaaact caccgcgcag attgtaacga
    44281 cacaggccgt tgtaaccgta gcggttcaaa tataaaaaca gtaccgcccg acggaacgga
    44341 tcctggcttt tgttgaactc ttcgcggaac tgatagtaaa cctcggcgca atttgtttcg
    44401 ggaacaaaca gctcgcgtgc ggcctgtacg tactcatcag tacgcatctt cacaatgtta
    44461 tagagactga tcaggtcgct attgatatcg gcaaggatat aacgagaaaa gtcggtgttg
    44521 agaaacaccg acccggcacc tacaaaaggc tcaaccagac attcgccctt gggcaaatgc
    44581 cgtttaatat catcaagcag gggatacttg ccccctgccc acttcaaaaa agcgcgattt
    44641 ttcttcatgc cgattaacta attacacctt ctccggctgt ggagaaagct ccgacagcat
    44701 ccagcgcttc aggcagctcc cgcaacgata aaccattgcg ggagataacc ttgattactt
    44761 cagatcggcc tgtacctgac gcagcggttt cgcccacggg tttttggcct gaacatctgc
    44821 tggcaatgta gataccgctt ttttcgcctc ttctttcgaa gcgtacacgc cagaaaccag
    44881 gacataccac ggctgaccat tacgcgtcgt ttcatagaca acgtagtttt tcagattctc
    44941 tttcttcgcc caaccgttca ggttgtcgta gttagaggaa ctgctcagct gcagagtgta
    45001 atggctggac ggtgccgatt tcaacgaacc aacattacct gcggtcttcc ctccagcggc
    45061 tggtgttgcc gtggtttgcg ccggggatgc cgtctgtact ggagccgtag tcgccgtctc
    45121 ttttggtgct ggcgtagaag tcgcagccgg tgccttcgtg ctcgccacag gggcagctgg
    45181 ttcagtacgc ttcggcgtct gtgctaccgg cttcggctcc gttttcacgg ttgcttgcgg
    45241 ttttttcggt tcaatcaccg cctgctgacg aactgggcgc gtagtggacg gacgttcagc
    45301 ggtttgcgtt ttcgccgagt cacgcgatgc attgccattg cgaacaggcg caaccgttgc
    45361 gggttcagtc ggcaacgtgg aattgaccgc cacattgttc agctgttgct gattttgtgg
    45421 ctgggtcagc gcattgttca ggtcaccctg cacttcaaca cgttgttgac catccgttgc
    45481 caccggggtt tgcccttgag tcggcgtaga agagatcggt ggcagcgaaa catcctgctg
    45541 agtattttcc gcagacgtgg ttcccggcgc tggctgcacg ccattcgcct gatcggtcgc
    45601 attgccagca agatcaatac tcttctcgcc agacgcggtt tgatcgctgg aagtggtcga
    45661 gggggctttt agcgcagaac cgataccgat gatcaacagc agtagaacca gaatgccgac
    45721 gcccatcatc atatactgac gagaagcggg tttactggct gcttttttgc gcttacgcgg
    45781 acgacgctct acgcgctctt catccacggt ttcatcttcg gattcgtcaa tttcttcttc
    45841 gatttccggt tcctcattgc gctctttttg cgcacgagtc ggacggcgat cgtcagtgtc
    45901 atcaagttca atatcatcaa aattgatctg cggttcgcca cgttcagtac gctcagaacg
    45961 ttcagaagat tgacgagaac gaccagtacg acgatcgctg ggatcgggtt tcagctcgtc
    46021 ttctggtttg aattcatcca tttaacaccc cactaaaagg ttaatgctta ccacgttgca
    46081 attaacctga agctaataga ccgcttgata agcggcctga cctttcttgt tgttacgctg
    46141 attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga aacgccgctg cgaacttcac
    46201 tcttaccaat tgccaacgga agaattaagc gcatctctcc cgcaaggact ttcttgtcac
    46261 gcagcatatg cggtaaatac gcctgcgcgg acatttcgcg cggcccattg accggtaacc
    46321 cagcccgcgt gagcagggtt ataatacgct gcgtttcggc agaactaaac tgcccgagac
    46381 gttccgacgt ccgcgccgcc atcaccatac ccgcagcgac cgcttcacca tgtaaccaat
    46441 tgccataccc catttcagct tcaatggcat gaccaaaggt gtgtcccaga ttcagtaaag
    46501 cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc tgccttcagt tcacaacaac
    46561 ggcgaataca gtacgccatt gccggaccgt ccagacgcaa caacgcatcc agattctctt
    46621 ccagccagtt aaaaaacgca ccgtcaagaa taatgccgta tttgatgact tctgccagcc
    46681 ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc gagatccacc accactgaag
    46741 caggctggta gaacgcgcca atcatgtttt taccgagggg atggttgacc gcagttttgc
    46801 cgccaacgga ggaatcgacc tgcgacagta acgtcgtcgg gacttgaatg aaacgaacac
    46861 cgcgctgata actcgccgcc gcgaagccgg tcagatcgcc cactacgccg ccgccaagcg
    46921 ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa cgccgtaaag acggtatcga
    46981 gtacagctag gcttttatac tgctcgccgt cagggaggat aacgctatcg acgttaacac
    47041 ccgcctgttc aagtacgccg cggaccttat cgagatacag aggagccagg gtttcgctgg
    47101 tgaccaacat gacctgctcg cccgatttca gcggtaagaa tgaagctggt tcattaaaca
    47161 aaccagatgc gatggtaatt gggtaactac gttccccgag agtaacgaca atcctctcca
    47221 taacgcgaca tccaccttaa ttactgtacc cgcagacgag tgtatataaa gccagaatta
    47281 gttgctttcc agcatgtgaa taatctggtt tgcaaccact ttagcgcttt gatcatcagt
    47341 acgaatggtc acgtcggcaa tctcttcata cagcggattg cgttcattgg ccaacgcttc
    47401 cagaacttca cgcggcggtg tttcaacgtg cagcaacggg cgttttttat cacgctgcgt
    47461 gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg acaacgccac gagcggaaag
    47521 acggttacgc gtttcacggg atttcacaga gccgccgcca gtagccagca caataccctg
    47581 tttctcggtc aactcattga tgaccttttc ttcgcgatcg cggaagcctt cttcgccttc
    47641 taaatcgaaa acccagccca catcagctcc ggttcgtttc tcaatctctt gatcggaatc
    47701 gtaaaattcc atattgagct gttgagctaa ctggcgccca atagtgcttt ttccggcacc
    47761 cataggccca accagaaaga tattgcgttt ctctgccatt ttttcggtac tactaagact
    47821 attcgttaat gataaacccg cttcgctcag agagcgccgc aggacatgaa ctgaaacctc
    47881 ataagatatt gcgagagtca gactgaaaat tatctcaata ctccagcggg tttggcaact
    47941 gaataaatca cccatccctc tgcatatctg gtcgctgcaa gcgcgctgcc ttgctaccac
    48001 cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa agacagcaaa atgccgcctg
    48061 aatgataaat catcatcatg gaacgcaggc agcgaagagc accaactctc aaatcggtac
    48121 tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa acacgttcag cataaaaaca
    48181 ttaccgcttt tacggctgtt tactcactgg aaaccagtcg tggcgtgata aacaccacta
    48241 actcgcgtcg ttcatcttct tttccgtcat gacgaaataa ttgcccgaac cagggaatgt
    48301 cgccaagcaa cggtacgcta tcctgacccg atttattttt acgggtaaaa atgccgccca
    48361 gcgccaacgt ttctccgctt tttacctcga cctgcgtttc gatctcctgc ttatcaatcg
    48421 ccagcacttc gccatcggcc tgctgtagca cctgccccgg aacgttctgg ctgatgtgta
    48481 atttcagccg gatgcgacct ttttgtaaca ccgtgggcgt gacctccatc cccaggacgg
    48541 cctctttaaa ttccaccgac gtcgcgccac tttccccgct ggaaacctga tatggaattt
    48601 cgctctcctg tttaatgctg gcaggctgaa gatgtgaggc cagcagacgc ggactggcga
    48661 taatatccag ctgctgtttt tgttcgagcg cggaaagctc aagatccagc aagcgtccgt
    48721 tgatgcgccc aatgttaaaa ccgacatgcg ttgtcgccgt cgctacggag aggtcgctac
    48781 caagcgtggt gacttgccca acgccaccag cgtgttgcgc atcggccagc gtccatttca
    48841 cgcctaactc acgcaaactt ttttcattaa tggtgacaat atgcgccgac agctcaacct
    48901 gcccgaccgg cagatccatt tgcgctaccc actgttcaag cgcgcttaac gccgttttgt
    48961 tatctcgtag caaaaggcga ttggtgcgtt tatcgacggt catactccct ttggcactca
    49021 gtagcttctc ccccgctttc gccagttctc ccgcgtcggc gtattgcagg gttatactgc
    49081 gattttccag cggcagattt gcctgcgccc gcgcctgctc cgcctcctgg cgggcgatat
    49141 tgtcattctg ccaggcaatg gaatgcactg agagaatgtt gccttcctgc cgcgttatca
    49201 gtccggcgct tttcactaca gtttgtagtg cctgcttcca gggaacatct gttagatgta
    49261 acgacaccgt accgctgacg tctggcgaca cgaccaggtt caacttctcc tgttcagcca
    49321 gcgcctgcaa cacctgagct accggaacgt catccaccat cagcgtcact ttttgcggct
    49381 ttgccgcctg tacgccgggt atcagcatca acagtagtgc ggctatccat tgcttcattt
    49441 gtatctcctt gccgttgcca caaccattgt ggcggttcgc agtttgtccc ggtacccagc
    49501 gttagtacgt ctggcgtcag ctgtaaaatt gtccagccgt tttccagcac atcgttttgc
    49561 tgcacccgtc gccatttctt ttgcccgtct tttattacac cgatgatgcg ctcgcctcgc
    49621 cctaccatcc cctgatagcg ccactggcta agttcgctaa tccggcatag atcttccggc
    49681 gatttaaaag ggtcacgcat accggttaaa aggcacaatg caatacctgc caacaaccag
    49741 cgtttaaccc tcatgcggca tctccagttg tagcgtgaac aaaagatcat caccttccac
    49801 gcttaacgaa aaacggctca cgctgacgtt gcgctctgcc agccgtgtaa atgccgacgg
    49861 cactgcttcc cacagcgttt tcaacgccaa ctcgcctccc tgcgcggatg gatgccagga
    49921 aaccagttgc gcgccggata actgaaaatc cagtggcgaa aagggcagcg ttttttcctc
    49981 gctgaaggga gtggtgtcta ccaggcgata cagtgcggcc cactgctggt gatgacttgc
    50041 ccgtagtcga attaatgcgt cgcgctcttc atggtgtgtc gatgatagaa aaatgagcgt
    50101 aactaacatc agcaaccaga atgcccagca aaactggcgg aggcggggtg atgtggcgaa
    50161 ccaccagtca aagaacatgt tcatcgctaa ccttccttgt taactgatac tcaaattgcc
    50221 agcgtccctg cgcatcctgc tgcgtggctc cccgctgatt gagatgaaaa gacgcatcct
    50281 ggcggagtga cgtttctagt gcgtttaacg cggtaatgct tgttgtcagc cccttgatct
    50341 ccagcgttcc ctgctgccag cttatcgttg tcagccaggc gtgctcgggt aaaagcgccg
    50401 ccagtgcttc cagcgcagat tgccagtcgc gggtaaattg tcgctggcgc tgccgttgcg
    50461 aacgctgttc gcgtaactgc tgttgctcca gcaaacgtgg cttcgttatc tgtaagctgc
    50521 gggcgagttg ttgttccgcc tgaagcaaaa ctgcgtctat gcgcgcttcg gcgctgcctg
    50581 tcagacgcag tattagcgtt atcccgacgg ccagcagcag aggcgcaacg aacatcagca
    50641 accagaaacg cagaaaagcg gtccggcgtt gctgtcgcca gggcaaaaaa ttaattggcg
    50701 ggttcatcag tacgccttcc caagcgccag ccccagcgcg atggcaaagt ctccaccagg
    50761 cggcggtagc ggcggctgac gaacagaaac ggcctcccag gggtcaaatc cgccttcgcc
    50821 acatatcgcg acgctgtcgg gatcaacgga taacgctgcc gccagctcct tcgcgctagt
    50881 catccccacc gccagtttgc gcccccagct atagcgcgtc gcccacagcc actgttcgtt
    50941 atcacgccag gccagacatt gctgatgaga aggtaaaaaa ggcaggaatc gctgtaatgc
    51001 actggcatcc ggggtgatcg cactaacatg aacacgcaac ctttctgcca gcgtaagcag
    51061 cgttgccagc tctttgcttt gcgcggcagt cacgttataa gcggggctga gtgagtcttc
    51121 gctgtaatcg aagcgcaggg agtccggatc catatccagc tcgcgggcca tcgtccctga
    51181 cagccaggcc gtttgctccc gctcaccaag ggacatcgac gggcgcggaa atgaccgctg
    51241 taatgtgcga ctggcgggaa acgccaacat aatgtgatga cgctgcggca gttcgcgact
    51301 ccacggtaac aacgttttag ccagctgctg cgcatcaaca atccgcccat ctttgataat
    51361 gtcgtgcgcc agcggcaacc gccaccagcg ttgcaaaaag cattcttttg cgccccgcac
    51421 gatcgcaacc gctgccgctt cttgctgttg taaatgcaaa ccaatttgcc agatcttaaa
    51481 tgtcattgtg atgatctcct tatcacccgt cactctgacg ggtatatcaa tgcgtctggc
    51541 ttgcctttat actaccgcgc gtttgtttat aaactgccca aatgaaacta aatgggaaat
    51601 ttccagtgaa gttcgtaaag tattttttga tccttgcagt ctgttgcatt ctgctgggag
    51661 caggctcgat ttatggccta taccgctaca tcgagccaca actgccggat gtggtgacat
    51721 taaaagatgt tcgcctgcaa attccgatgc agatttacag cgccgatggc gagctgattg
    51781 ctcaatacgg tgagaaacgt cgtattccgg ttacgttgga tcaaatccca ccggagatgg
    51841 tgaaagcctt tatcgcgacg gaagacagcc gcttctacga gcatcacgga gttgacccgg
    51901 tggggatctt ccgtgcggca agtgtggcgc tgttctccgg ccacgcgtca caaggggcga
    51961 gtaccattac ccagcagctg gcgagaaatt tcttcctcag tccagaacgc acgatgatgc
    52021 gtaagattaa ggaagtcttc ctcgcgattc gcattgaaca gttgctgacg aaagacgaaa
    52081 tcctcgagct ttatctgaac aagatttacc ttggttaccg cgcctatggt gtcggtgctg
    52141 cggcacaagt ctatttcggt aaaacggtcg accaactgac gctgaacgaa atggcggtga
    52201 tagccgggtt gccgaaagcg ccttccacct ttaatccgct ctactcaatg gatcgtgccg
    52261 tcgcgcggcg taacgtcgta ctgtcgcgga tgctggatga agggtatatc acccagcaac
    52321 agttcgatca gacacgcact gaggcgatta acgctaatta tcacgcgccg gagattgctt
    52381 tctctgcgcc gtacctgagc gaaatggtgc gccaggagat gtataaccgt tatggcgaaa
    52441 gtgcctatga agacggttat cgcatttaca ccaccatcac ccgcaaagtg cagcaggccg
    52501 cgcagcaggc ggtacgtaat aacgtgctgg actacgacat gcgccacggc tatcgcggcc
    52561 cggcaaatgt gctgtggaaa gtgggcgagt cggcgtggga taacaacaag attaccgata
    52621 cgctgaaggc gctgccgacc tatggtccgc tgctgcctgc cgcagtcacc agcgccaatc
    52681 ctcagggagc gacggcgatg ctggcggacg ggtcgaccgt cgcattgagt atggaaggcg
    52741 ttcgctgggc gcgtccttac cgttcggata ctcagcaggg accgacgccg cgtaaagtga
    52801 ccgatgttct gcaaacgggt cagcaaatct gggttcgtca ggttggcgat gcatggtggc
    52861 tggcacaagt gccggaagtg aactcggcgc tggtgtcgat caatccgcaa aatggtgccg
    52921 ttatggcgct ggtcggtggc tttgatttca atcagagcaa gtttaaccgc gccacccagg
    52981 cactgcgtca ggtgggttcc aacatcaaac cgttcctcta caccgcggcg atggataaag
    53041 gtctgacgct ggcaagtatg ttgaacgatg tgccaatttc tcgctgggat gcaggtgccg
    53101 gttctgactg gcagccgaag aactcaccac cgcagtatgc tggtccaatt cgcttacgtc
    53161 aggggctggg tcagtcgaaa aacgtggtga tggtacgtgc aatgcgggcg atgggcgtcg
    53221 actacgctgc agaatatctg caacgcttcg gcttcccggc gcaaaacatt gtccacaccg
    53281 aatcgctggc gctgggttca gcgtccttca ccccaatgca ggtggcgcgc ggctacgcgg
    53341 tcatggcgaa cggcggcttc ctagtggacc cgtggtttat cagcaaaatt gaaaacgatc
    53401 agggcggcgt gattttcgaa gcgaaaccga aagtagcctg cccggaatgc gatattccag
    53461 tgatttacgg tgatacgcag aaatcgaacg tgctggaaaa taacgatgtt gaagatgtcg
    53521 ctatctcccg cgagcagcag aatgtttctg taccaatgcc gcagctggag caggcaaatc
    53581 aggcgttagt ggcgaagact ggcgcgcagg agtacgcacc gcacgtcatc aacactccgc
    53641 tggcattcct gattaagagt gctttgaaca ccaatatctt tggtgaacca ggctggcagg
    53701 gtaccggctg gcgtgcaggt cgtgatttgc agcgtcgcga tatcggcggg aaaaccggta
    53761 ccactaacag ttcgaaagat gcgtggttct cgggttacgg tccgggcgtt gtgacctcgg
    53821 tctggattgg ctttgatgat caccgtcgta atctcggtca tacaacggct tccggagcga
    53881 ttaaagatca gatctcaggt tacgaaggcg gtgccaagag tgcccagcct gcatgggacg
    53941 cttatatgaa agccgttctt gaaggtgtgc cggagcagcc gctgacgccg cctccgggta
    54001 ttgtgacggt gaatatcgat cgcagcaccg ggcagttagc taatggtggc aacagccgcg
    54061 aagagtattt catcgaaggt acgcagccga cacaacaggc ggtgcacgag gtgggaacga
    54121 ccattatcga taatggtgag gcacaggaat tgttctgatt aaaaaggcgc ttcggcgcct
    54181 tttcagttgg ctgacaaagt gcatttgttt atgccggatg cgggtgaacg cgttatccgg
    54241 ctaacaaaat cgtgaaaaat caatatattg caggaacccc ataagcctga taagcgttgt
    54301 gcatcaggca aacttcacgc atttacactc gcccctgccc tttcaaccat tcgcgcacga
    54361 ggaacagcgc gctgacatta cgcgcttcgt tgaagtcagg gtcttccagc aaatccatca
    54421 tatgcgccag cggccagcgc acctgtggta gcggctctgg ctcatcgcct tccagcgatt
    54481 ccgggtagag atcttgcgcg accacgatat tcattttgct ggaaaagtaa gacggtgcca
    54541 tgctgagctt cttcaaaaaa gtcagatcgt tcgcaccaaa tccaacctct tcttttagct
    54601 cgcggttagc ggcttcgtag acgctttcac ccggatcaat taatcctttc gaaaaaccta
    54661 attcgtagga ttcagttccc actgcgtatt cgcggatcag gatcaggtga tcatccacaa
    54721 tcggcacaat catcactgct tcccggttgg ttggacgcat tcgttcataa acacgccgca
    54781 cgccattgct gaactccaga tccacgctct cgacggtaaa cagtcgggaa cgggctacag
    54841 tttcaacatt cagaatggtg ggtttttgta atgatttgtt catcgtggga tctatgctgt
    54901 gaaatcagcg gttattgtgc gatatcggac acgctttcgg caatgtgaat tgcatgttat
    54961 ttacatttat gtaacttaat aaataatcgt cctcaaatca aattaaaagt caataggttg
    55021 aaataactcc aggaatttgc tgatattccg ccttcagagg gtttgctatg atcagcggtt
    55081 actgtgatgt gcttaatgat gctcaagtta agctccacgc ttgccgatag ccaaccgcag
    55141 aatcatgtat tgtgtccggt gcgactgacc acgcctgaca gactaagtaa gatggggaaa
    55201 gcatgagcac cattgtgatt tttttagctg ctttgctggc ctgctcacta cttgcgggat
    55261 ggctgataaa agtgcgatcc agacggcgtc agctgccctg gaccaacgcc ttcgcggatg
    55321 cgcaaacgcg taaactcaca cctgaagaac gtagcgccgt tgaaaattat cttgagagcc
    55381 tgacgcaggt attacaggtg cctggcccaa cgggagctag cgcggcaccg atctctctgg
    55441 cgctgaatgc cgaaagcaac aacgtcatga tgctgacaca cgctatcaca cgttacggca
    55501 tctctaccga cgatccgaat aaatggcgtt actacctcga ttcggtagaa gtccacctgc
    55561 cccccttctg ggaacagtac atcaacgatg agaataccgt tgaactgatt cataccgatt
    55621 cgctgccgtt ggttatttca ctcaacggtc atacgctgca ggagtacatg caggaaacgc
    55681 gcggctatgc cttgcaacct gttccgtcca cgcaggcgtc gattcgcggg gaagagagtg
    55741 agcaaatcga gctactcaat attcgcaaag aaacgcatga agaatatgcg ctgagtcgtc
    55801 cgcgcgggct gcgtgaagcg ttgctgatcg tcgcctcctt cctgatgttc tttttctgcc
    55861 tgattacccc ggatgtattt gttccgtggc tggcaggcgg cgcattactg ctgctgggcg
    55921 caggtctgtg ggggctattc gcgcccccgg caaaatcctc cctgcgagaa attcattgtc
    55981 tgcgcggtac accccgtcgt tggggattgt ttggcgaaaa cgatcaggaa cagatcaaca
    56041 atatttcact cggtattatc gacctggtct atcccgcaca ctggcagcca tacattgctc
    56101 aggatctcgg tcaacaaacc gatattgata tctatctcga ccgccacgta gtgcgtcagg
    56161 ggcgatatct ttcgctgcat gatgaagtaa aaaactttcc gttacagcac tggctgcgca
    56221 gtacgattat cgctgcgggc tcgctgctgg tgctgtttat gctgttattc tggatcccgc
    56281 tggatatgcc gctgaaattc actctctcat ggatgaaagg cgcgcagacc attgaagcca
    56341 ccagcgtaaa acaactggct gacgctggcg tgcgggtagg cgatacattg cgtattagcg
    56401 gtacgggaat gtgtaatatt cgcacttccg gaacctggag cgcgaaaacc aattcacctt
    56461 ttttaccgtt tgactgctcg cagatcatct ggaatgacgc ccgctcatta ccattaccag
    56521 aatctgaact ggtcaacaaa gcgacggcat tgactgaagc agttaatcgc cagctgcacc
    56581 ctaaaccgga agatgaatct cgcgtcagtg cctcattgcg ttcagcgatt caaaaatccg
    56641 gcatggtatt gcttgatgat tttggcgaca ttgtactgaa gacagcggat ttatgttctg
    56701 ccaaagatga ctgtgtgcga ctgaaaaatg cgctggtcaa tctcggcaac agtaaagact
    56761 gggacgcgct ggtaaaacgc gccaacgccg ggaagctcga tggcgtgaat gtgttattac
    56821 gcccggtgag tgcggaatcg ctggataacc tggtggcaac ctccaccgcg ccgtttatca
    56881 cgcatgaaac ggcgcgagcg gcacaatcac taaacagtcc ggcccccggc ggattcctga
    56941 ttgtcagcga cgaaggcagc gattttgttg atcagccctg gccttcggca tcactttacg
    57001 actacccgcc gcaagaacag tggaacgctt tccagaaact ggcacaaatg ctgatgcata
    57061 cgccgtttaa cgccgaaggt atcgtcacaa aaatcttcac tgacgccaat ggtacgcagc
    57121 atattggcct tcatccgatc ccggatcgtt ccggcctgtg gcgctatctc agcaccacat
    57181 tgctgctact gacgatgctg ggtagcgcca tttacaatgg cgtacaggcc tggcgtcgtt
    57241 accagcgtca tcgcactcgc atgatggaga ttcaggccta ttatgaaagc tgcctgaacc
    57301 cgcaactgat caccccttca gaaagcctta tcgaataaca cgtttgcgcg gcaggttatg
    57361 ctaccctgtc gcgcaaattg cttcactctg gagatttccc tcatgcatat caacattgcc
    57421 tggcaggacg tagataccgt tctgctggat atggacggca cgttgctcga cctcgccttc
    57481 gataactatt tctggcaaaa gctggtgcct gaaacatggg gcgcgaaaaa cggggttacg
    57541 ccacaggaag cgatggaata tatgcgccag caatatcacg acgtacagca tacgctaaac
    57601 tggtactgtc ttgattactg gagtgagcaa ctgggtctgg atatctgtgc gatgaccacc
    57661 gagatgggac cgcgcgccgt actgcgtgaa gataccattc cgtttcttga ggcactgaaa
    57721 gccagcggta agcagcgaat tttgctcacc aatgctcatc cgcacaacct ggcggtaaaa
    57781 cttgagcata cgggtctgga cgcacacctt gatttattac tttccaccca cacatttggt
    57841 tatccgaaag aggatcagcg gttatggcat gcggtggccg aagctacggg tctgaaagct
    57901 gaaagaacgc tgtttattga tgacagcgaa gcgattctcg atgctgccgc gcaatttggt
    57961 attcgttact gcctcggcgt gactaatcct gattccggga ttgccgagaa acagtatcaa
    58021 cgccatccgt cactgaatga ctaccgccgc ctgatcccct cgctaatgtg aaggagacgc
    58081 catgaaagag aaacctgctg ttgaggttcg actggataaa tggctatggg ctgcccgttt
    58141 ttataaaacc cgcgcgctgg cccgtgaaat gattgaaggc ggtaaggtgc attacaacgg
    58201 gcagcgcagc aagccgagca aaatcgtcga gctgaatgcc acgctcactc tgcgccaggg
    58261 aaatgacgaa cgcacggtga ttgtaaaggc gattactgaa cagcgtcgcc ccgccagcga
    58321 ggcagccttg ctgtatgaag agactgcgga aagtgtagag aaacgcgaaa aaatggcgat
    58381 ggcacgtaaa cttaatgcct taaccatgcc gcacccggac cgacgcccgg acaaaaaaga
    58441 gcgccgcgac ctgttacgat ttaaacacgg cgacagtgaa taactgtcac ctgcaagaga
    58501 gatgattatg ccgcaacatg accaattaca tcgctatctg tttgaaaatt ttgccgttcg
    58561 cggcgaactg gtaaccgttt cggaaaccct gcaacagatc cttgagaacc acgattatcc
    58621 gcagcccgtt aaaaacgtgc tggcagaact gctggttgcg accagcctgt taaccgctac
    58681 gctgaagttt gatggtgata tcaccgtaca gctgcagggc gacggtccga tgaatctggc
    58741 ggttattaac ggtaacaata accagcaaat gcgcggtgtg gcgcgcgtgc agggcgaaat
    58801 tccagaaaat gccgacctga aaacgctggt cggcaatggt tacgtggtga tcaccattac
    58861 cccgagcgaa ggcgaacgct atcagggcgt agttggtctg gaaggtgata ccctggcggc
    58921 ctgcctagaa gattacttta tgcgttctga acagctgccg acgcgcctgt ttattcgcac
    58981 cggcgacgta gacggcaaac cggctgcagg cggtatgttg ttgcaggtaa tgcctgcgca
    59041 aaatacccaa caggacgact ttgaccacct ggcgacgcta accgaaacca tcaaaaccga
    59101 agaactgctg accttaccgg caaacgaagt gttgtggcgt ttgtatcacg aagaagaggt
    59161 gacggtttac gatccgcagg atgtggagtt caaatgcacc tgctcgcgtg aacgttgcgc
    59221 cgatgcgctg aaaacgctgc ctgatgaaga agttgatagc atcctggcgg aagatggcga
    59281 aattgacatg cattgtgatt actgcggtaa ccactatctg ttcaatgcga tggatattgc
    59341 tgaaatccgc aacaacgcgt ctccggcaga tccgtaagtt cattaatgag tatgtccggc
    59401 agagagggct ctgccggatt ttacgactta atggcctgat gtgctgcgct taccagcgcc
    59461 tatgaaataa atcgcaaatt attttggtca gatacagcgt cgtatccgat aaaagcgttg
    59521 tcacttactt aagtttattt ccagcgtttc accatctacc gtcagatcgc gccacgcttt
    59581 cggcctcgtc cccagttttc catccttcgc agctgtgagt aatttttctt tcgttatttc
    59641 gttcggaagt aaactcatat caagcgcgtc ccattctttt ctgtctgagt caaagccata
    59701 gacaatgact ctgtcatcat taaaagcaaa caggatccgc tccgcctgac catcactatt
    59761 cagatcttgc tcgaccagta cgcaggcgtc tttttcaata caggtcatca cgttatagcg
    59821 atcctggatt aaggccgacc agaatgtcgc atccggttta acagaaccag gggcaattaa
    59881 cacattttcg gcgaatactt tttccgatac ctgttcctga agatgttgct ctccatctaa
    59941 cgccatcagc agatcccgcg cgcgtttcgg gtctttcata aacccggcat cgcttttcag
    60001 cgactcaagc gccgcacgtc catagcgacc gctctgttcg agcatgtaga tagttacctg
    60061 gtctggcgtg tttttgccgc tctgataacg cgccatatga ctgttcacgc taatacgcat
    60121 actgtccagc accggcgaat taagaagcac cagtatcacc aacaccaata acgaaaccgc
    60181 aaggttcact ttgccctgaa gaacaagggg attttgccca ttacgccaga cgatactgac
    60241 aaaatacccc agcgaccaca ccagtaacac cagctccgcc agcgcgccct gcaagcggtc
    60301 gacagtccag ccgtactgag cgacccgcag ccataacccc caggtggcga cgaacacata
    60361 cagcggagca accagcaaag cggttttaat caggcaacgc aacggccccg tccagggaag
    60421 tgacgctttt tgcggatcgc ggacaatagc cattaagatc aattgcaaaa aggccagcgt
    60481 taacagcaac ccggcggcgg agatgtggcg agaaatcgcg ctcaggcccg taaacggcag
    60541 ggtgatgata aacatcgggg ttagcaatga tactaacggc agcaaccccg tggcgattag
    60601 cgtgaacaac ttttgaatag agtcgattaa acgtgactgt gtacgcgcga ggatcaccgc
    60661 cagtgcggta accagaccaa atgtaagata cataaaccag tcggttgcaa aaaagagcgt
    60721 attgaaaaac gtgatgccaa caagtttaaa caactcaccc cacagtaaaa gcaccagcca
    60781 cgtcaggcca ttggagagaa aaatcaccag taatataagt acgttatgcc atactgactg
    60841 gtaaaagtag ctatagcggc tactgccatt gcggatacga aggctttgtt gtatccacgg
    60901 tagcaacaac attgccatca acagcagata gcagccaaaa tcccaaatcg ccttttcagc
    60961 tctccagggg ttcatgccat cagtttgcca cttcagccaa ccgctcatcc ctgtcgtggc
    61021 aataaacacc agcgccagcc atccccaaag gcgcttctgt ttaaaagaga tcacggagaa
    61081 gagtaaaacg gatgaaaacg cgacggtcgc aggcacgcca taaacaatcc aactgtgatt
    61141 ttttccggca agccaggcga taagcaggta gcaaaccagc ccctgaagta atccggtagc
    61201 aatcatcccc cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa
    61261 tcaaaaggtg ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa
    61321 tcaaaaaaag ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt
    61381 caggaatgcg attccactca caatattccc gccatataaa ccaagattta accttttgag
    61441 aacattttcc acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa
    61501 tcacggagtt ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg
    61561 cggttaacac ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt
    61621 gaaatgcgcg ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac
    61681 gtacatgata tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg
    61741 agcctgacag gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc
    61801 gggatcttca ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc
    61861 gatactttct ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg
    61921 gaaacctggc agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc
    61981 gttgtcgacg ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc
    62041 gaagtggcct ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag
    62101 ctggcaggtt tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag
    62161 tggaaagaac agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg
    62221 cagctgattg gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg
    62281 aactacctgc tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag
    62341 aaaggcgatg ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc
    62401 gacccgaaac gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt
    62461 aatttcgaag gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa
    62521 atctacaacg ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc
    62581 actatcgact ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat
    62641 cacatcgata acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc
    62701 ctgactgctg atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc
    62761 cagtatcact tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc
    62821 gaaccgacgc caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact
    62881 cagtacgcag aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt
    62941 aacactggct ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc
    63001 gacgccatcc tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac
    63061 ctggcgatcc caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc
    63121 tacgcttctc cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac
    63181 aacttcgata aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa
    63241 ctgtaatgat ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag
    63301 gcaccttcgc ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg
    63361 cactggcagc caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat
    63421 cccgttatgg ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat
    63481 ggtgcgcgca ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc
    63541 aattcccgga ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct
    63601 gactttgatc cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg
    63661 tttgatcgac agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat
    63721 ttcccgctca tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc
    63781 catttccatc ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc
    63841 gttgcactct tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat
    63901 catctcagtc gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc
    63961 catcagcagc gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc
    64021 acgggtaacg gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc
    64081 tttaccaacc tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat
    64141 aaacagccac gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg
    64201 agagaaatcg ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag
    64261 ccaggttttc agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg
    64321 gccgcccagt tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag
    64381 acctgcctct tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc
    64441 ccgacggaaa gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt
    64501 catcaacata cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat
    64561 cgcgaagttc agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat
    64621 gagcaataac gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta
    64681 gagccgtccg gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc
    64741 gctggatctt cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc
    64801 attgcggaat attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca
    64861 cgcggatggc tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc
    64921 tcatcttcgc ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt
    64981 accgcctctt cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca
    65041 cgaatacggg ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca
    65101 atctccaggc ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc
    65161 atcgggttgc tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac
    65221 attaaatcca gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct
    65281 gcattagcga cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca
    65341 cgcaggcgca tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact
    65401 cccaaaggtt cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca
    65461 gctaaatctg gtataaattt cagcctaaat tgttacaaag catattaaac agcagcttaa
    65521 gtatacaatt tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa
    65581 tgtgcgcatt atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg
    65641 ggaagggtat gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga
    65701 ggtcacaaaa aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta
    65761 tcagtataat aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg
    65821 cctggaaaat ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tattttttgg
    65881 cgcgtgggtg gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta
    65941 cgatgaaatt tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt
    66001 gctgaaaaaa gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg
    66061 gtatgttaat gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg
    66121 gctggcattt ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg
    66181 aactcaaacc atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg
    66241 cccggaacag gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat
    66301 cgcacgttat cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga
    66361 aacgcgtctg agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat
    66421 ttccgagcaa ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa
    66481 aaccgaactc gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat
    66541 cgccattgaa gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac
    66601 gccagaagtc gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc
    66661 cgcgctggat ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct
    66721 ggcgaaagtg cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg
    66781 taaagaagag gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac
    66841 ggtgccttct caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct
    66901 ttcgctgaat gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat
    66961 catcatggat caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag
    67021 cgtagtgagc tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg
    67081 taccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga
    67141 tctgctgatg gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg
    67201 taccggggta aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat
    67261 ttacccgcac accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa
    67321 acataacgtt gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt
    67381 ctatctcgac gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga
    67441 agctggcgcg tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga
    67501 cgtttcgctg cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct
    67561 ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac
    67621 gcaactggcc cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga
    67681 tctcaacacc gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc
    67741 gcaaaacatc gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct
    67801 gaaagtcagc cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa
    67861 ccacggtgat aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga
    67921 acgcattctg gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact
    67981 gcgtaacctg aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga
    68041 catcatcaaa gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca
    68101 gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc
    68161 agtgaccaac gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct
    68221 ggttcacatc tcttcattat cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc
    68281 gggcgacatt gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct
    68341 gactatgcgt ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga
    68401 acgcccgcaa aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg
    68461 taatagcgcg atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg
    68521 actggcccct aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc
    68581 ctgacataaa agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat
    68641 atactccgtt aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag
    68701 tccgggtaac tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa
    68761 cattgagtca gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga
    68821 aaccattctc attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat
    68881 ctggtctcat gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag
    68941 taggcaccta tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa
    69001 atcagcccgg catatcgcca aaaattgctt tctctaggca tgttacctgg ctcctctttt
    69061 gatgtggtgc gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc
    69121 ctggtattac gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac
    69181 ggtgataaca acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa
    69241 gacaacctta tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt
    69301 taccgtcgaa cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga
    69361 cctgcccggc acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat
    69421 cgcctgtcac tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc
    69481 taaccttgag cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat
    69541 cgtggcgctg aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc
    69601 tctatcggca cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat
    69661 tgaagcgctc aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca
    69721 ttacgcacag ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat
    69781 cccgctgaaa caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg
    69841 cgcctacgcc ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga
    69901 aatggatgat ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg
    69961 tgatgtggta agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa
    70021 aatcgtgctc aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt
    70081 cctgctggct atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc
    70141 gctatttgtg catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac
    70201 tatcttcctc gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca
    70261 gatcggcatg atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc
    70321 agcgtttgtg atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc
    70381 gctgatcgtc ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc
    70441 accgcgtgaa cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct
    70501 ggctatcttc gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt
    70561 ctcgctgtat atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac
    70621 catcatgcgc ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca
    70681 cgttaaaagc ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg
    70741 taaagtgatt atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg
    70801 gaaaatcgtc gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc
    70861 tgtcttcaag ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac
    70921 gggcgcaatg gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa
    70981 tattcaggac gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc
    71041 ggtagatgaa acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc
    71101 cattgaagct agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca
    71161 gaaattcggt agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc
    71221 atgtatctcg gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc
    71281 catcctgtgg gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag
    71341 ctacagtcag cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat
    71401 cgtagctatc ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac
    71461 ccgcaagtcg gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct
    71521 tcacttattc aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata
    71581 agccagacat tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc
    71641 atgggcaaag ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa
    71701 agctgcccgg aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc
    71761 atcgcctgtt ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt
    71821 ccataaaaat gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat
    71881 gaaaactcgc taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag
    71941 gtgaaggaac gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact
    72001 ctttttacgc caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat
    72061 tcacgcgctc tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga
    72121 tctgcgtgaa agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata
    72181 catctattgt ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat
    72241 gcgttacgct attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat
    72301 ggtggtgcca ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg
    72361 gctggattgt ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct
    72421 gattgatgtc accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct
    72481 ggagttaatt caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc
    72541 ctgcttatta agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat
    72601 actgcaaact ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc
    72661 accgaaacac aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca
    72721 atccaaagcc ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat
    72781 catgcgattt accgggctgt cagaagaaga gttg
//
LOCUS       DAJTUN010000004        71332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTUN010000004 DAJTUN010000000
VERSION     DAJTUN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.004"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(750..2345)
                     /locus_tag="OO470_000233"
     CDS             complement(750..2345)
                     /locus_tag="OO470_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6249618.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="OO470_000234"
     CDS             complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="OO470_000234"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6249619.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4893..5297)
                     /locus_tag="OO470_000235"
     CDS             complement(4893..5297)
                     /locus_tag="OO470_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6249620.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="OO470_000236"
                     /pseudo
     CDS             complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="OO470_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="OO470_000237"
     CDS             complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="OO470_000237"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6249621.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="OO470_000238"
                     /pseudo
     CDS             complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="OO470_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9334..10111
                     /gene="glcC"
                     /locus_tag="OO470_000239"
                     /pseudo
     CDS             9334..10111
                     /gene="glcC"
                     /locus_tag="OO470_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
     gene            complement(10235..11376)
                     /locus_tag="OO470_000240"
                     /pseudo
     CDS             complement(10235..11376)
                     /locus_tag="OO470_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11738..13468
                     /locus_tag="OO470_000241"
     CDS             11738..13468
                     /locus_tag="OO470_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6249622.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13465..14379
                     /locus_tag="OO470_000242"
     CDS             13465..14379
                     /locus_tag="OO470_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6249623.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14412..14660
                     /locus_tag="OO470_000243"
     CDS             14412..14660
                     /locus_tag="OO470_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6249624.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14660..15831
                     /locus_tag="OO470_000244"
                     /pseudo
     CDS             14660..15831
                     /locus_tag="OO470_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15867..16946)
                     /gene="lptG"
                     /locus_tag="OO470_000245"
     CDS             complement(15867..16946)
                     /gene="lptG"
                     /locus_tag="OO470_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6249625.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16943..18013)
                     /gene="lptF"
                     /locus_tag="OO470_000246"
     CDS             complement(16943..18013)
                     /gene="lptF"
                     /locus_tag="OO470_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6249626.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18044..18604)
                     /locus_tag="OO470_000247"
     CDS             complement(18044..18604)
                     /locus_tag="OO470_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6249627.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18616..19440)
                     /locus_tag="OO470_000248"
     CDS             complement(18616..19440)
                     /locus_tag="OO470_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249628.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19440..20789)
                     /locus_tag="OO470_000249"
     CDS             complement(19440..20789)
                     /locus_tag="OO470_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6249629.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20835..21593)
                     /locus_tag="OO470_000250"
     CDS             complement(20835..21593)
                     /locus_tag="OO470_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249630.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21625..22338)
                     /locus_tag="OO470_000251"
     CDS             complement(21625..22338)
                     /locus_tag="OO470_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6249631.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22512..23204
                     /locus_tag="OO470_000252"
     CDS             22512..23204
                     /locus_tag="OO470_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6249632.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23253..24752)
                     /gene="pitB"
                     /locus_tag="OO470_000253"
     CDS             complement(23253..24752)
                     /gene="pitB"
                     /locus_tag="OO470_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6249633.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25044..26903)
                     /gene="gss"
                     /locus_tag="OO470_000254"
     CDS             complement(25044..26903)
                     /gene="gss"
                     /locus_tag="OO470_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6249634.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27108..27974
                     /gene="yghU"
                     /locus_tag="OO470_000255"
     CDS             27108..27974
                     /gene="yghU"
                     /locus_tag="OO470_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6249635.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28097..28345)
                     /gene="hybG"
                     /locus_tag="OO470_000256"
     CDS             complement(28097..28345)
                     /gene="hybG"
                     /locus_tag="OO470_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6249636.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28479..28820)
                     /gene="hypA"
                     /locus_tag="OO470_000257"
     CDS             complement(28479..28820)
                     /gene="hypA"
                     /locus_tag="OO470_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6249637.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28813..29301)
                     /gene="hybE"
                     /locus_tag="OO470_000258"
     CDS             complement(28813..29301)
                     /gene="hybE"
                     /locus_tag="OO470_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6249638.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29294..29788)
                     /locus_tag="OO470_000259"
     CDS             complement(29294..29788)
                     /locus_tag="OO470_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6249639.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29788..31491)
                     /gene="hybC"
                     /locus_tag="OO470_000260"
     CDS             complement(29788..31491)
                     /gene="hybC"
                     /locus_tag="OO470_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6249640.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31488..32666)
                     /gene="hybB"
                     /locus_tag="OO470_000261"
     CDS             complement(31488..32666)
                     /gene="hybB"
                     /locus_tag="OO470_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6249641.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32656..33642)
                     /gene="hybA"
                     /locus_tag="OO470_000262"
     CDS             complement(32656..33642)
                     /gene="hybA"
                     /locus_tag="OO470_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6249642.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33645..34763)
                     /gene="hybO"
                     /locus_tag="OO470_000263"
     CDS             complement(33645..34763)
                     /gene="hybO"
                     /locus_tag="OO470_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6249643.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34952..35239)
                     /locus_tag="OO470_000264"
     CDS             complement(34952..35239)
                     /locus_tag="OO470_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6249644.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35358..36245)
                     /locus_tag="OO470_000265"
     CDS             complement(35358..36245)
                     /locus_tag="OO470_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6249645.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36402..37442
                     /gene="gpr"
                     /locus_tag="OO470_000266"
     CDS             36402..37442
                     /gene="gpr"
                     /locus_tag="OO470_000266"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6249646.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37482..37976)
                     /locus_tag="OO470_000267"
     CDS             complement(37482..37976)
                     /locus_tag="OO470_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6249647.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38167..39051
                     /gene="yghA"
                     /locus_tag="OO470_000268"
     CDS             38167..39051
                     /gene="yghA"
                     /locus_tag="OO470_000268"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6249648.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39323..39748)
                     /gene="exbD"
                     /locus_tag="OO470_000269"
     CDS             complement(39323..39748)
                     /gene="exbD"
                     /locus_tag="OO470_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6249649.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39755..40489)
                     /gene="exbB"
                     /locus_tag="OO470_000270"
     CDS             complement(39755..40489)
                     /gene="exbB"
                     /locus_tag="OO470_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6249650.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40741..41928
                     /gene="metC"
                     /locus_tag="OO470_000271"
     CDS             40741..41928
                     /gene="metC"
                     /locus_tag="OO470_000271"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6249651.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42068..42727
                     /locus_tag="OO470_000272"
     CDS             42068..42727
                     /locus_tag="OO470_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6249652.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42767..43666)
                     /gene="yqhC"
                     /locus_tag="OO470_000273"
     CDS             complement(42767..43666)
                     /gene="yqhC"
                     /locus_tag="OO470_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6249653.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43860..45023
                     /gene="yqhD"
                     /locus_tag="OO470_000274"
     CDS             43860..45023
                     /gene="yqhD"
                     /locus_tag="OO470_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6249654.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45128..45955
                     /gene="dkgA"
                     /locus_tag="OO470_000275"
     CDS             45128..45955
                     /gene="dkgA"
                     /locus_tag="OO470_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6249655.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46155..47081
                     /locus_tag="OO470_000276"
     CDS             46155..47081
                     /locus_tag="OO470_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6249656.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47132..47389
                     /gene="yqhH"
                     /locus_tag="OO470_000277"
     CDS             47132..47389
                     /gene="yqhH"
                     /locus_tag="OO470_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6249657.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47432..49651)
                     /locus_tag="OO470_000278"
     CDS             complement(47432..49651)
                     /locus_tag="OO470_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6249658.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49762..51174)
                     /gene="ftsP"
                     /locus_tag="OO470_000279"
     CDS             complement(49762..51174)
                     /gene="ftsP"
                     /locus_tag="OO470_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6249659.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51249..51986)
                     /gene="plsC"
                     /locus_tag="OO470_000280"
                     /gene_synonym="parF"
     CDS             complement(51249..51986)
                     /gene="plsC"
                     /locus_tag="OO470_000280"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6249660.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52220..54478)
                     /gene="parC"
                     /locus_tag="OO470_000281"
     CDS             complement(52220..54478)
                     /gene="parC"
                     /locus_tag="OO470_000281"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6249661.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55024..55506)
                     /locus_tag="OO470_000282"
     CDS             complement(55024..55506)
                     /locus_tag="OO470_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6249662.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55559..55951)
                     /gene="ygiW"
                     /locus_tag="OO470_000283"
     CDS             complement(55559..55951)
                     /gene="ygiW"
                     /locus_tag="OO470_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6249663.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56103..56762
                     /gene="qseB"
                     /locus_tag="OO470_000284"
     CDS             56103..56762
                     /gene="qseB"
                     /locus_tag="OO470_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6249664.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56759..58033
                     /gene="qseC"
                     /locus_tag="OO470_000285"
     CDS             56759..58033
                     /gene="qseC"
                     /locus_tag="OO470_000285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6249665.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58079..58411)
                     /locus_tag="OO470_000286"
     CDS             complement(58079..58411)
                     /locus_tag="OO470_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6249666.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58730..59311
                     /gene="mdaB"
                     /locus_tag="OO470_000287"
     CDS             58730..59311
                     /gene="mdaB"
                     /locus_tag="OO470_000287"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6249667.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59342..59656
                     /locus_tag="OO470_000288"
     CDS             59342..59656
                     /locus_tag="OO470_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6249668.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59704..61596)
                     /gene="parE"
                     /locus_tag="OO470_000289"
     CDS             complement(59704..61596)
                     /gene="parE"
                     /locus_tag="OO470_000289"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6249669.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61625..62206)
                     /gene="yqiA"
                     /locus_tag="OO470_000290"
     CDS             complement(61625..62206)
                     /gene="yqiA"
                     /locus_tag="OO470_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6249670.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62206..63033)
                     /gene="cpdA"
                     /locus_tag="OO470_000291"
     CDS             complement(62206..63033)
                     /gene="cpdA"
                     /locus_tag="OO470_000291"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6249671.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63058..63480)
                     /locus_tag="OO470_000292"
     CDS             complement(63058..63480)
                     /locus_tag="OO470_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6249672.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63481..64110)
                     /gene="nudF"
                     /locus_tag="OO470_000293"
     CDS             complement(63481..64110)
                     /gene="nudF"
                     /locus_tag="OO470_000293"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6249673.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64315..65796
                     /gene="tolC"
                     /locus_tag="OO470_000294"
     CDS             64315..65796
                     /gene="tolC"
                     /locus_tag="OO470_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6249674.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65944..66615
                     /locus_tag="OO470_000295"
     CDS             65944..66615
                     /locus_tag="OO470_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6249675.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66621..67781
                     /locus_tag="OO470_000296"
     CDS             66621..67781
                     /locus_tag="OO470_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6249676.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67819..68607)
                     /gene="ygiD"
                     /locus_tag="OO470_000297"
     CDS             complement(67819..68607)
                     /gene="ygiD"
                     /locus_tag="OO470_000297"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6249677.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68750..69523
                     /gene="zupT"
                     /locus_tag="OO470_000298"
     CDS             68750..69523
                     /gene="zupT"
                     /locus_tag="OO470_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6249678.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69581..69751)
                     /locus_tag="OO470_000299"
     CDS             complement(69581..69751)
                     /locus_tag="OO470_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249679.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70012..70665)
                     /gene="ribB"
                     /locus_tag="OO470_000300"
     CDS             complement(70012..70665)
                     /gene="ribB"
                     /locus_tag="OO470_000300"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6249680.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70769..70917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71039..71329
                     /gene="ubiK"
                     /locus_tag="OO470_000301"
     CDS             71039..71329
                     /gene="ubiK"
                     /locus_tag="OO470_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6249681.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17784 a        19035 c        17643 g        16870 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaat atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gccgataacg ctggcaaaaa tcagactgtg cttcacggtg
      841 tagcggaaca gttcagattc tcggcccacc atgcccgtcg cggcgcaggc cacggcgata
      901 gattgcgggg agatcatctt gccagttacg ccgccgctgg tatttgctgc caccagcaga
      961 gtgtcagaga cgttgatttg ctgcgccgtg gtcgattgca gtgaaccaaa cagggcgttt
     1021 gaagaagtgt ccgacccggt aaggaatacg cccagccagc cgagaaacgg tgagaaaaac
     1081 gggaacatca cgcctgtacc tgccagtacc agcgccagcg tggtggacat gccggaatag
     1141 ttggtgacga aggcgaatgc cagcaccatg ccaatcgaca atatcggcca cttcagacta
     1201 attagcattt cggcaaagac gccaatccct ttcttgatgc ccacgccgag gatgaagata
     1261 gagataatcg ccgcaataaa aatagcggtg ccgccagccg agagagggtc gaatttaaac
     1321 accgcatcca ttggcgttgg ttgggcgaca atgggtgccg ctttcaacac ttgttgatgc
     1381 aaatgaggga tctggaaatt aatcaccagt gaataaaacg cgccgcccgg agcaaataac
     1441 gctttaaacg gcttcatggt ccagatggtg accagcaccg ttaagattaa aaacggtgac
     1501 cacgcacgaa tgatttgccc cagactatat tctgaaggca cgggaccgcc agaagatggc
     1561 ttatttacca ccatcgcacc tgcggattgc cccatgctga ttgccgtttc ggtatttttc
     1621 ggccgccaga ctttaagaaa taaagcgagt gagacgatac tcaccagcgc cgaagtaata
     1681 tccggcagtt ccggaccaat atagttagag gtaaagaact gagtgacagc gaagcttccc
     1741 ccagcaacca gcgccgctgg ccacgtctct ttcacccctt tccagccgtc catcattgct
     1801 accagccaga acggcacaag aaccgacagg aacggtaact gacgtcccgc cattgcgcca
     1861 atgtggaacg gatcgattcc cgttacctga ccggcgacca gaatcggcac gcccaacgca
     1921 ccaaacgcca ccgtcgcacc aaagccagca gctccttcca gcaacgcacc aaaggagaaa
     1981 ccaatcagta acacctgcaa acgctgatcg tcggtgatgg agataaccaa gctgcggata
     2041 atatcgaact gcccgctggc aacggttaat ttatacagga acaccgccgc gacaataatc
     2101 cacgcaattg gccataatcc ataaataaag ccatagcccg cagcagcaaa tgccatatca
     2161 atcggcattt taaaggcgaa tatcgcaatc aggatagata atataagggt tattgctcca
     2221 gcgacatgtc ctttcagacg taataccgcg agtgcaacga agaagaatat tatcgggatc
     2281 agggcgacca gagcggatag ccccagtcct cccatcggca tatacatttg ggtccaggta
     2341 accatattgt tcagtctctt attatcttta tatgcttgat atacttaagg ttgtaataag
     2401 caaaagagga ctgaactgta aaatataggc gttatacttt acagcaacag tacgccgcta
     2461 acgcaattgc tacctctggc ataacaagta tatcgggtaa gggtttctgt tccgcacacg
     2521 caggcgcaga gtatcgttaa gatgtccata ttgttgtttt aggcccgcta gtaatgcgct
     2581 acgggtattt aatattgtta aaccctgata atcgctccgg ttatttccgg gataaatgta
     2641 ctaccgcagt tactatcata gccccgacaa taaaacttgc cggggctttt ttgacgctat
     2701 taatgacttt ctttttcgcg taaacgccag gcgtgtaata acggttcggt ataaccgttt
     2761 ggctgtttca cgccgaggaa gattaaatcg ctggcagctt taaaagcaca cgagttagcg
     2821 aaattccccg ccatcggacg ataagccgga tcgccagcat tttgctgatc aaccactttc
     2881 gccatattct ccagcgacgc ctgcacctgt tctttggtca gaataccgtg acgtaaccag
     2941 ttggcgatat gctggctgga gatacgcagc gttgcgcggt cttccatcag cgccacgttg
     3001 tgaatatccg gcacttttga acaaccaatc ccctgctcca cccaacgcac cacgtacccc
     3061 agaatcccct gcacgttgtt atccagctct tgttggatct cttgcgccga ccagttagcg
     3121 ttttcagcca ccggaatagt cagcagatcg tccagcagcg gttcaaattc agcactgaac
     3181 tcggtctggg caatgttggc ttgcacgctc tgtacgttgg tatggtggta gtgcagcgca
     3241 tggagcgtag cagcggttgg tgacggaacc caggctgtgt ttgccccggc acgcagttgg
     3301 tcgcccttct ggctgtacat gtctgccatc aggtccggca ttgcccacat gcctttaccg
     3361 atttgcgctt taccgcgcag cccacagaac agaccggaaa gcacgttatt acgttcgtag
     3421 gctttgatcc acggcgtcga tttcatctgg tttttacgca gcatcgggcc agcttccatc
     3481 accgaatgca tttcatcgcc ggtacggtcg aggaaaccgg tattgatgaa cgccacgcgg
     3541 ttgcgcgcct gagcgataca gctacgcaag ttcagcgagg tccggcgttc ttcatccata
     3601 atgcccattt tcagggtatt cggtgccata ccgagcattg tctcaatgcg ggtaaacagt
     3661 ttgttggcga acgccacttc ctgcggaccg tgcattttcg gtttcacaat atagacgctg
     3721 ccagtgcgcg agtttttctg cacttttaaa tcatagaggg caatcgcgcc agtcatgacg
     3781 ccatcaagaa taccttccgg gatttcattg ccttcgctgt cccaaatcac aggaatggtc
     3841 atcaaatgac ccacgttgcg gataaacagc aacgagcgtc cgtgcagaga aatttcagag
     3901 ccatcggcgg cggtgtactg acgatcgtca ttcagtttac gtacgatttg ccgaccgttt
     3961 ttctccattt tctcttgcag agtcccctgc atcaggccca gcagattgcg gtacagcagg
     4021 attttatctt ccgcatcaac cgccgcgacc gaatcttcgc agtcgagaat ggtactgata
     4081 gcagcttcga cgataacatc gttgatgtgc gccggatcgt ctttgccaat ccgcccgttg
     4141 gcatcaattt gcagttcaat atgcaggccg ttatttttca gcaaaatgca ggtcggcgca
     4201 gcggcatcgc cacggtaacc gacaaactgt gctggagtac gtaacgtggt ttctttacca
     4261 tttttcaact ggatgcgtaa ttgtttatcg accaccttaa acgccaccac atcctgatag
     4321 ctgccgtttt ccagcggtag tgattcatcg aggaaacgtc gaacccaggc gataacctgc
     4381 tcaccgcgct gcggatcgta gccactgacc atcgcccctt cctgcgggat gatgtcgctg
     4441 ccgtataacg catcgtacag tgagccccag cgagcgttcg ccgcgttcag cgcgtagcgg
     4501 gcgttcattg ccggaaccac cagctgtggc cccgcctgac tggtgatttc gctgtcaatg
     4561 cccgtggttt ccaccgtcac gcgctccggt tgcggcacca ggtagcccag ttcacgaagg
     4621 aaagatttat aggccgcttt atcttttacc ggccccggat tgctgcgatg ccactcatca
     4681 agcgccgcct gaatgcgatc gcgttctgcc agcaactgac gattttctgg tgccagatcg
     4741 tgaacgatct catcaacatt gtgccagaat gcagcagcgt ccagttccac gcccggcaaa
     4801 acttctccat ccacaaaacg tttaaaattg gcgtcaatgc gtaaacggcc ctgggttatg
     4861 gtttgactca ttgtttctct cctcgttttc gcttatttcg ccaacaccgc tgctgccact
     4921 ttcgcgacct gcgcatcctg tgctccggtt aaaccagaaa cgcccacggc accaataatt
     4981 tgcccatcaa caacaaccgg tacgccgcct tccagcgacg tcagtaacgg cgcagtcacg
     5041 aacgcggtac gtccgttgtt caccatctct tcatagccct tagtttcacg acgccccagc
     5101 gcggcggtac gcgctttctc ctgggagatg taagccgcaa tcggcgcgca atcgtccatg
     5161 cgacttaacg ccagcagatg accgccgtca tcggcaacag caatggaaac agaccagtta
     5221 tttttctgcg cctcttcctg acctgcggca ataattgcac tcgccatttg ctggctaaga
     5281 atgactttag ttttcatttt tttattcctt ttcaagggct tgttctacaa tttcaatcca
     5341 gtgacgcaca gaggtacgac cggcgctcgc cagatgcgtc tggcaaccaa tgttggcggt
     5401 gacgatcatt tccggtttgc cgctttccag cgcattcatt ttgttatccc gcagctggcg
     5461 tgccagatcg ggatgcgtta acgcatatgt tcccgctgaa ccgcagcaca gatggctgtc
     5521 gggaacgtcc gttaaggtaa atccaagacg aagtaacact ttttccactt cgccgttcag
     5581 cttttgcgca tgttgtaggg tacacgggca gtggaaggcc agttttttat cgccgcgaat
     5641 tgccagtttt tccagcggtt cctcgcgcag aagttcgact aaatcgaccg ccagttcact
     5701 gacctgacgc gctttatcgg catataacgc atcgtttttc agcatctgcc catactcttt
     5761 gacgaacgcg ccgcaaccgc tggcggtttg caaaattgcc tcggcacctg cttcaatcgc
     5821 gggccaccag gcatcaatat tattgcgcgc ccgtgccaac cctttctcct gcgcattaag
     5881 atgatagtcc accgcgccac aacagcctgc ttcgttagct gacatgacgc tgatccccag
     5941 acgatccagc actcgcgcag ttgccgcgtt ggtgttgggc gaaagcgtag gctgggcgca
     6001 gccttccagc attaaaaccc gacgcttatg gcgcagcggc ggacgcggtt tagctttcac
     6061 cgtttcagca ggcagttttg ctctgacctg ttccggtaaa aacggtcgca gcaccagccc
     6121 tacctgcgtc agcgcacgga agaccgccgg acgcggcact acctggcgca atccttcgcg
     6181 cagcattcgc tccggcagtg ggcgtttcac tttctgctcg acaatatcac gcccgatatc
     6241 cagcaaattg tgatagcgca caccagaagg acaggtggtt tcacaattgc ggcaagtgag
     6301 gcagcgatcg agatgctcct gtgttttaag cgtgacttcg ttgccttcca gcacctgttt
     6361 aatcagatag atgcgcccgc gcggcccgtc cagttcatcg cccagaagct gataggttgg
     6421 gcaggttgcg gtacaaaatc cgcagtgaac acaggcgcac aggatgctgt cggcttccag
     6481 cgcgcgttct gccgcatctc ttcggttaat cgggtttgca tagcctgctc ctcaaagttc
     6541 cgcgtacatg cgaccggggt taaacacgcc gcaagggtcg agctgctgtt taagctgctg
     6601 gtgatagcgg aataaaggag ccgatagcgg ggcaaagcca ccatctccgg cactaaagcg
     6661 ggtcgcatga ccgccagcgt tgcgggcgat gcgatggatt tgattgtcat cggctgtcga
     6721 tttcagccag cgtaacgccc cgccccagtc gatcagttgc tcgccgggta aatccatcat
     6781 cggcgcatcg ctgggtaatg aaatgcgcca taaggtacct ggtaacgaga agaacggcag
     6841 ttgttgttca cgcaattgct gccagaactg acccgcaacc tcttcgccac ccagcagttc
     6901 acgcgctgct tttaccgatc cttcgccgcc ctcaaggcgg atccacaacg cattgtcgaa
     6961 gtaacataag ccactaatgg gtaatggctg gagttgccac tcggcgattt cactcatggc
     7021 ttcttgcagg ctgatttccc gacgcaggct cagagaggca cgcggtcgcg gtaacacttt
     7081 cattgagatt tcagtgagca cgccaagaca accatagctt ccggccatta accgtgagag
     7141 atcgtatccg gcaacgtttt tcatcacttc gccaccaaaa cgcagatgtt ttccagtgcc
     7201 ggtaatgatg cgcgtgccga ggacaaaatc gcggaccgaa ccgctccacg ggcgacgcgg
     7261 ccccgccagc ccgcaggcga ccatcccgcc ccaggtggct tcttcaccat aatgcggcgg
     7321 ctcgcaggga agcatttgcc cggcgctctc cagcgccgct tcaatcgcca ccagcggcgt
     7381 tccggcacga gcggttatca ccagctcggt cgggtcgtaa ttaacaatgc cgcgatgaca
     7441 acgcacatcc agcgtttgcc cggtgactgg gcgacctaaa aaggctttgc tattgctgcc
     7501 ctgaatcacc agcggcgttt tatcgctaat tgcctgattc acctgctcca gcagcgcctg
     7561 actgtaatca cactcgcgta gcatcaaaag cgctccagtt caggaaaagg taaatgaccg
     7621 tgatgcacat gcatggcacc aaattcagca cagcggtgta gcgtgggaat gtttttccca
     7681 gggttcagca aaccatcggg gtcaaacgcc gccttgaccg catggaaggt cgtgatttca
     7741 tcgctgttga actgggcgca catttgattg attttttctc gcccgatgcc atgttcccca
     7801 ctgatgctgc cgccaacttc aacgcagagt tcgaggatct tcccgcccag ctcttccgcg
     7861 cgggcaaatt caccaggttc gttggcatcg aaaaggatta acgggtgcat gttgccatct
     7921 ccggcatgaa agacgttggc aacacgtaaa tcatattgct gcgataaacg ggcaatgcct
     7981 tccagtacgc caggcagggc gcgacgcggg atggtgccat ccatgcagta gtaatccggg
     8041 gagatacgtc ctaccgccgg gaacgcattt ttgcgaccgg cccagaaacg tacgcgctct
     8101 gcttcgtcct gtgccaaacg gacgtcagtc gcgcccgctt tcaacaagat gtagttaacc
     8161 cgctcgcagt cttcctgtac gtcagactcc acgccgtcca gttcgcataa caaaatcgct
     8221 tcggcgtcga cgggataacc ggcatgaata aaatcttccg ccgcgcggat cgacaggtta
     8281 tccatcatct ccagcccgcc ggggataatg ccattggcga tgatgtcacc aaccgcaagt
     8341 ccggcttttt ctaccgagtc aaagctggct aacagaaccc gcgccacggg cggcttcggc
     8401 agcagtttta ccgtcacttc ggtggtcacg ccgagcatac cttccgatcc ggtgaacagc
     8461 gccagcaggt caaaaccagg tgaatccagc gcgtccgatc ccagcgtcag cgcctcgccg
     8521 tccagcgttt gcacttcaat tttcagcagg ttatgtacgg tcagaccata tttcaggcag
     8581 tggacgccac cggcattttc cgccacattg ccgccaattg aacaggcgat ttgtgaggaa
     8641 ggatccggcg cgtaatagag attatgcggc gcaacggcct aggagatcgc caggttacat
     8701 acgcccggct gcacgcgcgc gcggcgacca acggggttaa tgtcgaggct ctctttaaag
     8761 cgcgccatca ccaacaacac acctttttcc agcggcagcg cgccaccaga aagcccggtg
     8821 cctgcaccac gggtcaccac cggtacacgc aggcgatggc agacagccag aatcgctgtc
     8881 acctgttcca tttgcttagg cagaacaacc agtaatggac gcgtgcgata cgcgctcaac
     8941 ccgtcacact cgtaaggaat gatctcctca tcggtatgca ggatctcaag tccagggaca
     9001 tgctcacgca gtgccatcag taccgatgtg cggtcgacat cgggtaaagc gccatcaaga
     9061 cgctcttcgt acaagatgct catgagtagg cttcgctttg ttgtgttgtg tggcagctga
     9121 tttttgcgcg ctgcttctgt gaacagttat taagcgggct tttcgttttc gtctatctct
     9181 ttagctaccg gtcagaccat tttttttcca gctctgtgac cttgtcttgg ttaacttaat
     9241 gttaaattga tgtaacataa tcacttgcgt gatgtgcgtg ttttgcgagt taagaacaga
     9301 aaaattggtc ctacctgtgc acgaggtccg ggaatgaaag atgaacgtcg ccctatttgc
     9361 gaagtggttg cagagagtat cgaacggtta attatcgacg gcgtactgaa ggtcggtcag
     9421 ccgcttccct cggaacgtcg actgtgtgaa aagctcggct tctcacgctc cgcactgcgt
     9481 gaagggctga ccgtgctgcg cgggcgcggg attattgaaa cggcgcaggg tcgcgattct
     9541 catgtcgcac ggcttaatcg ggagcaggac accagcccgc tgatccatct gttcagtacg
     9601 cagccgcgaa cgctgtacga tctgctcgac gttcgcgcat tactggaggg cgaatcggca
     9661 aggctggcgg caacgctggg aacgcaggct gattttgttg tgataacccg ctgttatgaa
     9721 aaaatgctcg ccgccagtga gaaccacaaa gagatttcgc tgatcgaaca tgcgcagttg
     9781 gatcacgctt tccatctcgc catttgtcag gcttctcaca atcaggtgct ggtgtttacg
     9841 ctgcaatcat tgaccgatct gatgtttaat tcagtgtttg ccagcgtaaa taatctctac
     9901 catcgaccac agcaaaaaaa gcagatcgat cgccagcatg cgcggatcta caacgcggtg
     9961 ttgcagcggc tgccgcacgt cgctcagcgc gcagcgcgca gcgcgcgatc atgtacggac
    10021 cgtgaaaaag aatctccacg atatcgagct ggaaggccac catttgattc gctcggcggt
    10081 gccgctggag atgaacaaag tgggaatgtg agcagagttt taccctatcc ccaaggcgag
    10141 gggaccgccc ctgcacttaa caggatctgg tgttaaatct gttcatctat accctctccc
    10201 gaatgaccgc ccctgcgcta aacaggatct ggtattaaat ttgcttttca tacaatcggt
    10261 aacgcttgta cggctccgcc ccaatgcgtt ccagcatgtt attcatgcct gtattggttt
    10321 cgaggatcca tgacatctcc agcgcatcga tcttccggcg ggcaaacgga tcgcgtaagg
    10381 cttcaatcaa taacagcgca atcaccgggc cgatgcggct gaactgatac tcgtcgcgca
    10441 cgcccatcag cggtactcgc gcagttcgca caccgctgac tttcaaacgc cacagcaatt
    10501 ttgcccagcc gaagggaaag agcgatccgt tcagatcggc aatcgcctcg ttgatgttag
    10561 gcaagccaca ataaacgcgc agggtgcaga atcaatctca gcgatgtaga tcatatcgtc
    10621 cggcaccaga tatttaagtt ggtcgcccat ggtcgcgaat tcatgttcgg taaacggcac
    10681 aaatccccag ttgtgctgcc agccagagtt gaaaatctca cgcaggatct gcatctcttc
    10741 ggcaaaccgc tgacgattga tgcagcgaat ggtcaccttt ttgcgcacct gatccatcag
    10801 tttttttagc gccggagaga aagtgagatc ggttcgctgc atccaccacg ccagtaaatc
    10861 aatgcctttg tgataaccca gttgttcaat atgcgcggca taccacggtt tgccgtgtgg
    10921 catcatcgca cagggtggtg tgtcaaaacc ttcaatcagt aatccgcttt cctgattgat
    10981 attcaggctg aaaggcccgc tgatcttact tgcaccttgt gacttcaacc acgcttccgc
    11041 tgcgccaaac aacgcggcaa aatcctgcgg atcatcaatg gcgtcaatca tgccgaaatg
    11101 accggtatct tcaccgtaac gctcacggtg caaggtatct atttgcgcgg taatacgccc
    11161 aactatctgc cccgcttttt ttgccaccca cgcctgccag atgatatggt ccgtccccgg
    11221 atttttcgca gacaaatgct cgttgcgttc aatgaataaa gggggtatcc agtttggatc
    11281 gtcgggataa agtgatgacg gaaaagcgat aaatgcctta aggtcatttt tattaattac
    11341 tttttcaatt ttaatcatca gcagatcgca ttccatatct ctgtggataa caaataatta
    11401 atggtgaaaa acaggttgca acagcaacga gcggcatctt aataatcaac tcaatgaata
    11461 caaacgacga ataaatagta ttaattaatg attatgataa ggtcagaggc tacacagaaa
    11521 cgcctcaatg ccttttgtca atttaaggcg accggacaag gcttcttctt aatatattca
    11581 taaattaaat atcattcata ataaaaacaa aaaacttaaa ctcattattt aaatcaatgt
    11641 tcattttcac accgatgaaa atactatctc atttatcttt tatgcctata taatccaccg
    11701 cattattgtc attttatttt tgatatccaa ttttttcgtg gtgtatatgt ctaataaaat
    11761 ctttacgcat tccctaccta tgcgctatgc cgattttcca acgctggttg atgctttgga
    11821 ctacgccgct ctgagtagag ccggaatgaa tttttatgac agacgttgcc aacttgaaga
    11881 tcaactggaa tatcagacat taaaaacgcg tgccgaagct ggtgcgaagc ggttgttatc
    11941 gctgaacctg aaaaaaggcg aacgcgtggc gctgattgcc gaaacgagta gcgggtttgt
    12001 agaggctttt tttgcctgtc agtatgctgg cttagtcgcc gtcccgttgg cgattccaat
    12061 gggcgtcggt cagcgggatt cctggagcgc caaactccag ggtttactgg caagttgcca
    12121 gcccgcagcc attatcactg gtgatgagtg gttgccactg gtcaatgccg cgacgcataa
    12181 caaccccgaa ttacatgttt taagccacgc ttggtttaag gcattaccgg aagccgatgt
    12241 tgcgctccag cgtccagttc cgaacgatat cgcctacctc cagtacacct ccggcagcac
    12301 ccgttttccc cgtggcgtca ttatcaccca tcgcgaagtg atggctaatc tacgtgctat
    12361 aagccacgat ggcattaaat tacgccctag cgaccgctgc gtctcctggc agcctttcta
    12421 ccatgatatg ggactggtcg gctttctcct gccccccgtc gccacgcaac tttcagtaga
    12481 ttatttgcgc actcaggatt ttgccatgcg tcctttgcaa tggcttaaat tgatcagtaa
    12541 aaatcgcggc accgtttccg ttgcgccgcc gtttggctat gaattgtgcc aacgccgcgt
    12601 gaatgaaaaa gatctcgctg aactggatct ttcctgctgg cgcgtcgctg gtattggtgc
    12661 tgagccgatc tccgcagaac aactccatca attcgctgaa tgtttccgtc aggttaactt
    12721 tgacaataaa actttcatgc cgtgctacgg actggcagaa aatgcgctgg ctgtcagctt
    12781 ctctgatgaa gcctccgggg ttgtggttaa cgaagtggat cgcgacatcc tcgaatatca
    12841 gggtaaagcc gtcgcgccgg gtgcagagac acgcgccgta tcgactttcg tcaactgcgg
    12901 caaagcgttg ccggaacatg gtattgaaat ccgcaatgaa gcaggtatgc cggtcgcgga
    12961 acgtgtggta ggccatattt gcatctccgg tcccagtctg atgagcggtt actttggcga
    13021 ccaggtttcg caagacgaga ttgccgcgac gggctggtta gacaccggcg acctcggtta
    13081 tctgctggac ggttatctgt atgtcaccgg acgcattaaa gatctgatta ttattcgtgg
    13141 ccgtaatatc tggccgcagg atattgaata tatagcggaa caggaaccgg aaattcattc
    13201 tggcgatgcg attgcttttg ttaccgccca ggaaaaaatc attttgcaga tccagtgtcg
    13261 gatcagcgac gaagaacgtc gcgggcagct tatccacgcg ctggcggcac ggatccaaag
    13321 cgaatttggc gtgaccgcgg ctatcgatct gttgccgccc cacagtattc cccgaacgtc
    13381 ctccggcaag cctgcccgtg cggaagcgaa aaaacgttat cagaaggctt atgctgccag
    13441 tcttaatgtg caggaatccc tggcataaat caaactgtcg cggtgacggg cgctaccggg
    13501 tttatcggta aatatattat cgataacctg ctcgcccgcg gctttcacgt tcgcgcattg
    13561 acgcgtactg cccgcgctca cgtcaatgat aatctcacct gggtgcgcgg ttcgctggaa
    13621 gatactcatt cacttagcga gctggttgcc ggagccagcg tagtggtcca ttgcgccggg
    13681 caagtgcgcg ggcacaaaga agagattttc acccgctgta acgttgacgg cagcctgcgc
    13741 ctgatgcaag cagcaaaaga gagcggcttt tgccaacgtt ttctgtttat ctcttcgctg
    13801 gcggcgcgcc atcccgagct ctcctggtat gcaaaatcca aatacgtcgc cgagcaacga
    13861 ctggcagcaa tggcggacga aattacgctg ggcgtttttc gcccgacagc cgtgtatggt
    13921 cccggcgata aagagttaaa accgctgttt gactggatgc tacgcggcct gctgccacga
    13981 cttggtgcgc cagatacaca actctctttt cttcacgtca ccgattttgc gcaagcagtg
    14041 ggtcagtggt taagcgccga aactgtacag acgcaaacct atgaattatg cgatggcgtc
    14101 gccggtggct atgactggca acgcgtacga cagcttgccg ccaaagcccg ttgtggttcc
    14161 gtgcgaatgg ttgacattcc tctgccggta ctcacaggcc ttgcggatat cagtaccgcg
    14221 ttgagtcgcc tggcaggtaa agaacctatg ctgacccgct cgaaaattcg tgaattaacc
    14281 cacgccgact ggtcggcaag taataaccgt atttctgaag atatcaattg gtttcccggg
    14341 attagcctgg aacacgcatt acgcaacggg ctattttgat tgtcttgttt taaaggtgtg
    14401 ttaacgtttc tatggtaaat cgtgaaatag taatggatta catcctttcc tgtttacagg
    14461 atttggtcga aaacggtgtg gaaattaagc ccgacagcga tctcgtcaac gatcttggcc
    14521 tggagtcgat caaagtgatg gatctcttaa tgatgctgga agacagattc gatatttcca
    14581 ttcctattaa tattctgctg gatgtcaaaa cccctgcgca attaatggaa actctacttc
    14641 catggctgga gaataaataa tggggctata cgataaatat gcgcgccttg ctggcgagcg
    14701 tctgcaattt agtgacaacg ggttaacacc gtttggcacc tgtattgatg aagtgtattc
    14761 cgccaccgaa ggccggatcg gcaataaaaa agtgattctg gccgggacca ataattatct
    14821 cggtttaacc tttaatcatg atgccatcag cgaaggtcag gccgcgttag cggcccaagg
    14881 caccggcacc accggttctc gtatggcaaa cggcagttat gcgccgcacc tggcgttaga
    14941 gaaagagatt gccgagtttt tcaatctccc taccgctatc gtttttcaac cggttatacc
    15001 gctaatttag gcgtcatcag cgcacttgcc gaccataacg ccgtggtgtt actggatgcc
    15061 gacagccacg ccagtattta tgacgcctgt tctctcggtg gcgcggaaat tattcgtttc
    15121 cgccataacg atgctaaaga tctggaacgg cgcatggttc gcctcggtga acgggcgaaa
    15181 gaggcgatta tcatcgttga gggcatctac agcatgctcg gtgacgttgc gccgctggcg
    15241 gaaattgttg atatcaaacg tcgtctcggt ggttatttga ttgtcgatga agcgcattcg
    15301 tttggcgtgt taggcgcaac ggggcgcgga ctggctgaag ctgtcggcgt tgaagatgat
    15361 gtggatatta tcgttggtac tttcagtaaa agtctggcct cgattggcgg ttttgctgtc
    15421 ggttcggaag cgatggaagt gctgcgctac ggcagccgcc cgtatatttt taccgcctct
    15481 ccttctccgt cctgcattgc gaccgtgcgc tcgtcgttaa gaactattgc cacacaacca
    15541 gagctgcggc aaaagctgat ggataacgcc aaccatctgt atgacggtct gcaaaaactg
    15601 ggttatgagt taagttcaca tattagcccg gtagtgccag taataatcgg ttcgaaagag
    15661 gaaggtttac gcatctggcg cgagcttatc tcgccgggcg tgtatgtgaa tcttatcctg
    15721 ccgcctgccg cgcctgcagg catcacgctg ctgcgctgta gcgtaaatgc tgcccacagt
    15781 cgtgagcaaa tcgatgccat cattcaggca tttgctactc tgaagcagta atgtgagccc
    15841 gccgtggtgt ttgcgccacg gcgggtttaa tgttgtctgt cataccaata caccaggctc
    15901 aacgccacca acacaaacgc taccggtaaa ccgagcgcgg taacaggtgc gcttaacgca
    15961 aatagcaatc ccagattaac catgatttga tagctgatcc aggtgagtaa gcccacgatg
    16021 acgcctacag cgagacggct acccatccca ggcgaacgcg gggcactaaa ggtaaacggc
    16081 acagccagca aaatcatcgc cagggtcagg atgggttgcc ccagtttttc ccacagtgca
    16141 aggcggtatt cgctgctggg ttgcccggtt tcctgcaaat aatggatgta atgattaagc
    16201 tgacggacag aaaaagtatt ccccggcatt gataactctt caagattcat actggtgaag
    16261 gccgattgcc aggcaagatt atctaatgtt tccagcgttt ctttgccatt cagccatttt
    16321 ttatgattca caccatgcag gacccacgtt ttgtcgtctt caatggtggc actgcgtgca
    16381 taaatgtagg attcaagcga gagatcgtcg cgataatgaa atatctccac gcccaccggc
    16441 tggccctgct cgttcagcga tttcaccgtc acaaattcat taccacgcct ggcccacagc
    16501 atattgccgg taatgtcatc gtcctccccc aacgcggtgg cggtcgattt gatttgcaac
    16561 gcctgttgct gcaatggcga cgccacccac tcatctatcg cgcctaacga aacagtccac
    16621 aatatcccgg caaccagcgc caccagtgca atacggaaga tagaaaaccc cgtgctgcga
    16681 atggcggtaa gctcactgtt tttcgataac tgccccagtc caacaatacc acccaataac
    16741 gcgataaacg gcgaaagttc gaccagtgtg cgcggtaagg tcattagcac caccagcacc
    16801 gcctgagtcc agcgataacc gcccgggctg acaccatcca gttcgttaat caggttgaag
    16861 gtggtaaaaa gcggcagcaa tagccctgca gcggcggcaa aaccgagaaa gagatgacgg
    16921 attaaatagc gactgaaaac attcatcggg aaaatttctg cagcaaagag aagtcacgtg
    16981 cgaccagtat cagcaggccc attagcatga gtccgggcac taaccacaaa ccaggaatga
    17041 ggggaatcgc gccgttagcc accagtgtac ggcagacgtc gccgccataa aaaatggcaa
    17101 taaacagcaa cgtcagtggc aataacgttg aaaatcgccc ttgccgcggt tttacccgac
    17161 ttaatgaaat ggccagcagc gccatcaaca atgcggtcag gccacggctt tgtcgccatt
    17221 gcagttcggc atgatcggca ggaaaggcgg agcgcgccag ctccgtgacc gatttcgctt
    17281 tgcgtttgac gttcgggctt tgatccagcg gattcagatg taattgcaga ttacggtaga
    17341 tctgttcgtt gtcgtcacgc ccctgatgat cgagaagata ggcagtgccg ttgtgcaaca
    17401 taacggtcgg tttctcgggt gatgggtcaa ccacatcaac cgaaccggcg cggaaaatac
    17461 gggttcgatt ggcagtagaa gtataaatca gcgcgtcagt caggcgatta ttatcctgat
    17521 caaccgtctg cgaaaggatc attcgtccgt tatcgttagt gttaaatttc tttgcccgca
    17581 actggcgaac atccagctcc gactgcgact gctgctccag ttgataaatc tgcgcataag
    17641 cccacggacg accatacatc gacagaaggg tgacaaaaat acttagcggt atcgccagat
    17701 aaagaacggc tttgtacaag cgtcccggac tgccccccgc cgcagagata gcggtaattt
    17761 ccgagtcggt atacatctgc cctagcgtca cgccaactga cacatacaga ccaacaggta
    17821 acaacatctc cagtgcaatc agtactttgt agaaaacgat atccagcaca acatcaagag
    17881 ctaatgtccc atttgccgct tcggtcagat aacgctgtgc ggagtagctg gcgaaaataa
    17941 agatcagaaa accgacgata atcagcacca gacggcgcgt accacgcatg atgtagtgtt
    18001 caaccagctt catctcacta acgccctcaa tcctttgagt tttttattca ttgatcagtt
    18061 tctgcgccaa cgcaatgaca tgatctacct cttccggcgt caggctgccg tcctttgccc
    18121 actgaatttg cccggcttta ttcagcacca gaatggttga actctgctcc ggcaagcgcc
    18181 aggcaacgcg tccctgtcca ttgccgtcaa taacgaattg cgcccacgga tagcggcgtt
    18241 tatttttttc gattttgcca acaacaaaat agccagtacc aaagatggca tcatcggtgt
    18301 tcacaatggt ggtgggctga aaacgatcct gcggaaagtt tgccgcttca accgctttaa
    18361 ttaacagtga gttctttttc ttcgccgact tacgcccggc gatatactga ataatgcgga
    18421 cttttcccgc taattgtgtg ctgttccact tttggtagtg aatttcgtcg tcgctattca
    18481 gaagcagctc gccgccttcc tggatataaa caggggtaac cgttttgcca gtgacgaaat
    18541 tgtgtgcctg cacacccggg gctaagagca tgataatcaa cgcaataatc aaacgagatg
    18601 acatcattgc tccggctata gcggaaaacg cgaaatcagc agctctaagc gctgacaaat
    18661 atcgctatcc agcgccaacg gtgcataata cgactggcgg ttttccatat catccgccac
    18721 catgtcgtcg tgtttatctc ccatcaggcc attaatggcg gcgaggaaac gggaaatctg
    18781 taacgcctct ttattttcag gatcaagcga tcttaaggtg tcgtaataag aaacctgctg
    18841 gctgatgatt tttcgcacat catgaaattc cctgccggtt agtcgcggtt tggcaataat
    18901 ctctttcagg gtatgcattt gcttttgccg ataggcaata aaactgctta cgctttctaa
    18961 ctggctatgg cgtaatgaat agttcaccaa cgaccagacc ggagagctta aataaatccg
    19021 taacaggttg ccgtaatacg acacaatatt tttcttaccg ttacgaaagc cgtcctgaaa
    19081 acgccccaga aaaacggtcg ttttgccgaa taaaaatccg gcctgatgct tctttaaata
    19141 cagccgttgc gcaaaacgca ggtgcttgta gcgcgctcgc atgtatttaa attgtttgcg
    19201 ctcatcagca gtcagctcat cgcctttggc ttgtttgcga actaaacgca gtaattcctg
    19261 acgatcaacg ccattgagcc agaattgcag gcataacgcg taattatcat gaatgatttc
    19321 ggtggtacaa cttagtgcaa tagtcggcgg caaactaacc tgtgcatcaa cgacatcatc
    19381 ttcttcaatc gcaacaaata gacgctcgct cgcctcaagc gggaaatatt ttctcatcat
    19441 caggatttct ctccggctgc gttataaagc aaggaatggc gctgaaaaaa tgcctttaag
    19501 gttggaatta acgagagcgc cacgtccaga cattgcccac cgaagtaagc aaacgctgct
    19561 cccagcacac caaacagatg acagaacatg aacagcaata cgatgtagcc gattgaggcg
    19621 atggtttgcg ccacgagaaa agcgcgctgt tttcccgcca tcaatagcaa tgactcctgc
    19681 gggaagccca gcatcgaaat cacaattgcg cccagcatca cctgaatcag gtcatacgct
    19741 tcgagatact taacgccaaa caccagcgaa atgagcggct tgccgacgat caacaccgcg
    19801 agagccacca gaataccgat tccgcccgcc agtaaaccgg acttcacacc taacaaccac
    19861 ggtctggtgg tgcgcggatc taaacgcatc acctctgggt aaaaactttt acccagcaaa
    19921 cccgctggcg ttccggcggc gtcgaagaat gtcatggcga ttttaaataa cccggcggca
    19981 gcgggtccta acacgatccc caccaacact gtgctgcacg agttacgcgc cgaccagatg
    20041 gagtgggcaa tgttggttga ccagacaaaa ctccacgcgc ctttaatgtg tcgggcagac
    20101 tcaaacagat tcaatttgaa ggcgttatgg atatttctac ggcgtaattc gcgcgcggca
    20161 aaccaccagt acatggtgcc gccaaccaga ttcgacacgt accaggcaat aacaaaaccc
    20221 gcaaaaccaa agtcaaaata ccaggctgcg acgctccccg ctgcgcgcag aaaaggtttc
    20281 gtcgcctgct gtacagcaat caaatcaaag cgatctaccg cacgcagaat gccggtcggc
    20341 gtggaggaag ccattgaagg aatgagcgtg caatacagcg ccgccagcca aaagctttgg
    20401 tcatccagac ctaatgaatg ggaaaggaat ggcagtaagg caatgccgcc gacaatcgcc
    20461 accgcgccgc tgacgatatc cagcgagaag gaaaatgaga cgacattgcg aaattgctgc
    20521 ggattattgt tggtcaaggc tggcgttccg tactgaacca ccagctgcca tgtctgaaac
    20581 ttaataaaat cgctgatcga cttggcgtac gattgcacaa tcaccagtac accaaacatg
    20641 gcgggcgtca tccctttacc ggcacacgag agcgccaaca gccccagcaa ggcgctctcg
    20701 acattactgg agcctaacca ggcgctattg cgaataatgg tgcgaaacgc gccatctgca
    20761 aaccaatgtt tgatgttaaa acccgccaat tcagcctgac cttatcgttg atagtaaaaa
    20821 atatcccgcc agccttaagt taaacttcgg cggtcagaaa cgatggcaac cagagaaacc
    20881 gccttctgta cctgttccag cacttcgctg tagggcgctc tggaatcaat ctcaagaatt
    20941 tttgtgccgt tatagccaat cgtcgacatg acgccgattt tgtcctgcag ctcggcatac
    21001 tcatggtcag gcttgcggga gatggcagta tcaatatcaa tccccaaacg aatgattaat
    21061 tcaggacggt attgcgccat tttttggtat aaactccgtt cgcgctgcgc cagaaacatg
    21121 ctgattttcc cggtcgcacg ttcgacgccg atccccggtc cgtcataata aaagcccgaa
    21181 atttcagcct gcgggaagcg atcgctgacc accagaacgc cactttgcgc cagtcgctga
    21241 accttgcgta gattcgccat tcttcgcagc gagaagcagt acataatcac cgccgcccat
    21301 agcgccggag atttggtttt catgctttgg gttttcgagg atttggccgc caatcgccgt
    21361 tccagccaga cgccaaccaa cggcaatcgt ttgattttgt cgccgtcttc gccggagagc
    21421 agccccagat agcggcgctc ggtttgccag tgttgttgca gcgatttcac caggtcggtg
    21481 gtcagcgtgg atttaccggt gccatcacac ccgaccaccg caatcagccc cggaatgtag
    21541 ttgggctgcg gcgcggttgt actattaaca gtttgtgttt gtagtgcatc cattcgttaa
    21601 atcatcccct atccattttg gtgatcagga aagcgcggcg tgaattgcgc gtagtgacgc
    21661 ttgcataatt tcatcggctg gttgccgacc atccagttca aggatcttgg cgccattgaa
    21721 tgtcagttgc ggcgtaacgg cgattttttc ctgtaacgct gccagttggt ggtcaggttt
    21781 acgcgcaaac gcggtttgtt catcaatgcc aagacgaatc aacaatacgg gtaaataaga
    21841 cgccatccat tggtacagct tcaactcgcg ctgccttaac atttttatcc aaccgttacc
    21901 gcccgtggtt tttgccaatt gcgggccatc aaagcgaaaa cccggcactt caacctgcgg
    21961 gtagcggtcg gtgatgagca gatagccttg ctggctttta cacaacattt tgcgaaactt
    22021 gtacgcccgc cagcaggaaa gcagatagat aaccagcgca gtaatattgc caggcggagt
    22081 taagggcttt tcgtgtacat gtgccgcttt acttcgcaga taacacccac aaggtgcgcc
    22141 aataacaggg agctgtgaaa tccattcgcc aattcgcccg gacgattgcc cgagataaat
    22201 gtgttctgtt ggcattcttg ctgccagttc atttaccagg cttgccgtga gggtcgattt
    22261 acctgaacca tcacatccta caatggcaat aacacgcact ggcgtggaat taattattga
    22321 catattacgt tgattcacga aaaacccggc agtaaataat gtattgaata ttacgtggtc
    22381 cgtgctcaga atatccgttc aaccttattg tgacaatggc taattgtgac attgacctga
    22441 attacgcttt cataaaaaca tattaaccaa ataaatattt ttaatggata tttaaattaa
    22501 aggatatatt catgcagtca ataacacctc cattaattgc cgttattggt agcgatggtt
    22561 caggcaagtc aacggtgtgt gaacatctta ttaccgttgt cgaaaaatat ggtgctgccg
    22621 aaagagttca tttaggaaaa caggccggaa atgtcggtcg tgcagtgaca aaattacctt
    22681 tgatgggaaa atccttacat aaaacaattg aacgaaatca ggtgaaaaca gcaaaaaaat
    22741 tgcctggacc agttccggcg ctggtaatta cagcgtttgt cgcccgtcgc ttactgcgct
    22801 ttcgtcatat gcttgcctgt cgtcgtcgcg ggttaattgt tctaaccgac cgttatcctc
    22861 aggaccaaat tcctggcgct tacgatggta cggtgttccc acctaacgtt gaaggtggtc
    22921 gttttgtctc atggctggca agccaggaac gtaaagcgtt tcactggatg gcgagccata
    22981 agcctgatct ggtcatcaaa ctcaatgttg accttgaagt tgcctgtgca cgtaaacccg
    23041 accataaacg ggaatcgctg gcgaggaaga ttgccataac gccacagtta acctttggtg
    23101 gtgcacaact ggttgatatc gatgccaatc agccactgga acaggtgttg gttgatgcag
    23161 aaaaagcgat tacggatttt atgaccgcgc gtggttatca ctagtcaaaa atggaaatgc
    23221 ccgatcgcca ggaccgggca ttttcaggaa ggttaaatca actgcaatgc tatccagtac
    23281 agcccaccag aaagaaaaat tgccgccggt aaagtaaaca cccacgccat caggatgctg
    23341 gttaccgttt tacgctgtaa cccaccgccg tccaccacca tcgtccctgc taccgcagac
    23401 gagaggacgt gtgttgtgga gacaggcatc ccaatataac tggcaaggcc gatagacact
    23461 gccgccgtca tttgtgccgc catgccttgc gcatacgtca tgccgcgctt accaatcttc
    23521 tcaccgatgg tcatcgctac acgacgccag ccaatcatgg tgccaatgcc gagcgccagt
    23581 gctaccgcca tgatgatcca caccggagcg tactcaatgg tgcttaacat atcgctgcgc
    23641 agttttttca gcaggttctg gtcttcttta ctgacgcctg gcagtttcgc cagcttcgcg
    23701 gaggtatcgg agatgcacag cataatgcgg cgcagctggc tgcgctgact cacgcttaac
    23761 ggctcgtaac tttccatatt gcctggcagc atcgttttaa cgcgcgcaat cgcatcaaag
    23821 gtatttgccg gatgacagtg aaactctgtt acttgcgtgc catcagtcga tgctgcaggc
    23881 aatggaggtt ccatcgcaat caacttctgc ggcagttcag gatgctgttg caggtagtgt
    23941 tcgaagttgg taacggcatc gcgggtacgg gtaatttcat agccggacgc attcatattg
    24001 acgacgaagc cagcaggggc aatccccacc agtaccagca ttaccaggcc gatccctttc
    24061 tgcccgtcgt tcgcgccgtg cgaaaacgcc acgcccgcag cggaaacaat cagcgcaata
    24121 cgcgtccaga atggcggttt acgtttgccc tttttctttt tgcgatcttc cggaatgcgg
    24181 tgaatacggt cacgcttttt cgtcccactc cagaaacggc gcagcaggaa aatcagacca
    24241 cccgcaatga ccaggccgac gataggggaa acaatcagcg aggagaaaat tttggttact
    24301 tcacgcaggt ttaacgcatc catcaccgat gagccggtta acagcgcgtt ggttaaaccg
    24361 atgccgataa tcgcaccaat caaggtgtgc gaactggagg ccggtaaacc aaagaaccac
    24421 gttcccaggt tccagataat cgccgccagc agcatggaaa agaccatcgc caaaccgtga
    24481 gttgacccca tattcagcaa caaatcgatt ggtaacatat ggacaatggc ataggcaacg
    24541 ctaagtccgc ccaataacac gccaaaaaag ttaaaaaatg ccgccatcac cacagcaagt
    24601 tgtggctgca tggcacgagt ataaataacg gttgccaccg cattcgccgt gtcatgaaaa
    24661 ccattgattg cttcgtagaa caacacaaat gccagagcaa gtaataacaa aagccccgta
    24721 tatatatcaa ggccaacaaa taaatttagc atatattaga ttacgccatt ttgaatttac
    24781 gaacggacgc attatcagtg actttaacgg catgggcaaa gtgaaatatc attttttaac
    24841 gataaaaaga gtgttatttc ttgtgtatca gacaataagc attcattcaa aacctttaga
    24901 aataatcaaa acacaggtat tccactggtt aatatattca catatgaaat gaataaatat
    24961 tggaatatat aaatattgaa tattttgatt aatccccgta ctaattattc ttcataatca
    25021 gtacggggtt gctacaacat gtattacttt ttcaccacaa ttaacggttc aatatcactc
    25081 tcttttttga tgaccagtga ttcatcaccg cgcaaacacg tcccaccgta gttgccgcca
    25141 acggtgaagg tacatacctg aatgtattta ccgtccactt tcggcaaaca ccacagttgc
    25201 tgatagatgt ttttctgctc ggcaaattta ccgctggttt tgtccagcac ctcttcatga
    25261 tggctgacca ggtcgatatt gctaccacag cgaccggcga tcggtttcac tgcgtaacct
    25321 gttttcacca attcatcatt aacagtgaaa tcggtatcca gcaggtaacg atggtgcggg
    25381 aacagcgacc agaggatcgg cagaatcgct ttgttgccgg ggatcaccgt ccacagcggc
    25441 tcaaagacca gcacttccgg gcgcagcaat acgtcgataa gacgcacttc gttttgcgga
    25501 tgaccggtac ggattggcac cgcagcaaac tcacggtcgc taacttcacg aatctgatca
    25561 aacgcggttt cccacgccca ggttttccac acgcagttaa ccagtcgccc ttccccatca
    25621 atcagttgcc cggcagcatc ccagcccagt tcatccagcc cgcgcaagat acgcgtttca
    25681 aagcccgcct ggtgcagcgc ctgctccata aactgcgcgt gatagttttc ctcgatatct
    25741 ttgtcctgca tgatatggac aaacggacgt gcacgactgt gtttccaggc accagccagt
    25801 tcgttaatca gcccttccgc cggattgaag ccgttgcctt tatagccctg ctccgcccaa
    25861 cgttcgagga tcaagcccgc ttcggtatga caggaggcgg agtcggcgtt gtattcgtaa
    25921 accttcaggc cacgctcatc catgcagaaa tccatacgac cagtgatcat atggtgacgg
    25981 cgacgctgcc aggagagacg caaacgtggc cagaggattt tcggaatgtc gaacagcgcc
    26041 agcaggttgt catctttcag caccttgtcg gttgcgtgaa gatacatcag atgcagctcg
    26101 ttggtggctt taattagctc ctgctcggca ctctcggtaa tggtgtagta atgatacgga
    26161 tcctgattga tcacctgacc gttggcctgc acataggcgt tttgtagcgg atctttttca
    26221 tccagccatt taccgtcaaa ctggcctttg ttttccagac gcgctccact gattttcagc
    26281 agctcgcctg caatttccgg ctgcggtaag ctgtattcag tatcttccgt ctggatcatc
    26341 cagcccagaa tggtggtgtc atcaaaagtg tctttaaggg tatagcagcc gttttcgacc
    26401 accatctcca gctcgcgcgt ccactgttgc ccttgcggca acggggaatg aatcacgttc
    26461 tgttccgcaa tacggacttt gttgccatgc aattgggtaa tgatggcgac atggccagtg
    26521 tctttaaatt caccgccttt atcccagata agaagcgcac ccgcgaccgg cgcacgcggc
    26581 gagccgttag gaaatgcctg caatggcagg atgttgtcat taaccacttc tcgcaggaag
    26641 cgcagcgaga aaatctccca cgccataccc acgtcagtaa agaccacacc gtaattcaga
    26701 aagagaaaac ggcgagcaaa ttcaacgcat tgccacttgt ggcccatata ttcgtcgtcg
    26761 atatagctac ggaatacggc gtcatcttcg tattcctgcg gatcgagaga actgtaatct
    26821 gaagagtaga ttgctacccc acctggggcg tagcccaata atgtcccgaa cggggcatcc
    26881 tggctggtcg ttcctttgct catcacttta cctttaacaa tacaacctaa gcagttggcg
    26941 taattgttgt gctctgatta cctgctcagc gacattaacc ggacagaggt cagactaatc
    27001 atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa ttcacattct gtacagcaag
    27061 tgacctgcta cactgcttca acactaccac tcagaaggca actcactatg acagacaata
    27121 cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc tggcggcgcg ttcgccaata
    27181 tcaatcgccc gttttctggt ccgacgcatg aaaaaacgct gcccgttggc aaacatccat
    27241 tgcaacttta ttcgctggga acgccgaacg gtcagaaagt aacgattatg cttgaggagc
    27301 tactggcgct gggcgttact ggtgcagagt acgacgcctg gctgattcgt attggcgatg
    27361 gcgatcaatt ctccagcggc tttgtcgaag tgaacccaaa ctcgaagatc ccggcgctgc
    27421 gcgatcatac gcataatccg ccgatccgcg tgtttgaatc tggttcgatc ctgctttatc
    27481 tggcggagaa atttggctac ttcctgccgc aggatctggc aaaacggact gaaacgttga
    27541 actggctgtt ctggttacag ggtgccgcgc cgttcctcgg cggtggtttt ggtcactttt
    27601 accattacgc gccggtgaaa attgagtacg ccatcaaccg ctttaccatg gaagccaagc
    27661 gcctgctcga cgtgctggat aagcaactgg cgcagcataa gtttgttgcg ggcgatgagt
    27721 acaccattgc ggatatggcg atttggccgt ggtttggcaa cgtggtgctc ggcggcgtgt
    27781 atgatgccgc agagtttctg gatgcgggaa gttataaaca tgtgcaacgc tgggcgaaag
    27841 aagtgggcga acgtccggca gtgaaacgtg ggcgtattgt taaccgcacc aacgggccgc
    27901 tgaacgagca gttgcatgag cgccatgacg ccagtgattt tgagacaaat acagaagata
    27961 agcgtcaggg gtaagggttg gtgttcgtcg cagcaagcca tccaggccgg ataaggcgtt
    28021 cacgccgcat ccggcaatcg tgcacaatgc ctgatgcgac gctgccgcgt cttatcaggc
    28081 ctacaaaagc taatcatcac gcgctggtaa tgtcgtaatc catttggcgc agtgcgtcta
    28141 atgtggcttt ggcttcatct tcgtcgatga tgctcatggc aaatccgacg tgtaccagca
    28201 cccactggcc cagcagatcg gcagggttac cttcgcaaat cagggcgata ttcacgtcgc
    28261 gcttgatacc acatacttca acctgcgcaa gctggtgaat atcttcaccg acagccagca
    28321 cctggcctgg aacgccaata cacattgtta actccggtta tttcgattgc cggacaaacg
    28381 cgaaaaagaa cctttagcgg gtgttccctc tcaccctaac cctctccccg gaggggcgag
    28441 gggaccgtcc gtgcgctttt taggcgttgt cctcagtctt attcaacttc aatacttttg
    28501 acgatcagcg aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc
    28561 gcatcgtgct gatgaatctc caccacctgg ctgcaatccc agcaccaggc ctgggcgggt
    28621 ttatagacga tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa
    28681 ctaaaacgga cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg
    28741 gtgacgcgct taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg
    28801 caaagcgaca actcatgcat tttcgctcct cctgcggcca aacagcaggg cgcgacgccc
    28861 tgcgtgtggt acatccggat tggtgactgg caacgaaagg atcattcgtg cgcagtcatc
    28921 cgtcaggcgt tggccttctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca
    28981 ggagaggtat tgcgaaacac cgtccagttc gccaacggta aaggtcatgg tgccatacgg
    29041 cagttgcaga ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg
    29101 gaagataact gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa
    29161 cagcgtaaaa tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat
    29221 cgaacgccgg gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa
    29281 acctgctatc tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc
    29341 gcagcgcagc cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta
    29401 agccgatgtg tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa
    29461 actcaccggt gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga
    29521 ttttgttggt aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggag
    29581 tgttcttttt cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc
    29641 caagcagctc cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt
    29701 atcgttgctc taacgcttcg acaatccgca cgccgatggc ttcatcggtc agcaaaatat
    29761 tgccgacccc taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc
    29821 gtcggcatcc actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg
    29881 caccacttcc agcggtttat tcggatcggc aaccggtgta cccaccagcg actgctcgta
    29941 aggaccgacg tcatcattga agttacgcgg accagagttc caggttgatg gaacaaccgc
    30001 ctggtagttg ctgatgatac cgtctttaat aaccatccag tgagagagca taccgcgcgg
    30061 cgcttcgagg aagccaacac ctttgaactc acccgttgcc ggaatgttcg gcttcacaaa
    30121 ggtggtgtga tcgcctttgc cgatattggt gatcagtgca ctgtattggt tttgcaggat
    30181 atcctgcaat tcacagcagt gaacggtacg accaataata cggcccagcg tggagtgcag
    30241 ctgtgccact tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag
    30301 tttgttctgg gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc
    30361 cacttctacc gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc
    30421 agaccaacca tcataagccg gaatggtggt accttcccac ggcgcctgtg gcgcttcgtc
    30481 tttataccag gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg
    30541 agaagtgatc ggacgatacg aggacagatc cgcattctca atgtagcctc ccgggaacag
    30601 gaagctgccg tttttgctgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc
    30661 tttaccgcgc gtcagccatt ccgggtagaa cgcggcgata accgcggtat caaccttata
    30721 aacctgctca acaaagtcgc tcagtttgtc gatgaaagac ttgatgtaca tcaggcgctc
    30781 aaggttcaac acgcccaacc cgtcgaggtt gattgggttc gcgacaccac ccactgccag
    30841 gttctgaatg tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg
    30901 gcactccagc gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt
    30961 catcgccggg tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag
    31021 atctttgatc ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga
    31081 aacgcctttc agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc
    31141 cacccagtcc agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc
    31201 agccagaatg atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact
    31261 ttctgccgca cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg
    31321 cacaatcatc catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca
    31381 catggtaccg gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg
    31441 taaatgcccc tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc
    31501 gcctcatgac gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg
    31561 agtacgatgt aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc
    31621 caggtcggga agtaggcgta accgccgccc gggttgaatg ccaccaacga ataggtcaga
    31681 cgccaggttg cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca
    31741 ttacgcagct tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc
    31801 cagaacatca cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata
    31861 agctcgccaa agcgcagccc gatgaaaatc gccagcaaca cactgatggt gttggtcagc
    31921 ttgacgaaca ggctcttttc atccggaccg ttgccacgca gacccgcctg caccagtgaa
    31981 ccttcaaaga tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc
    32041 aacatttcat agctctgcca gagcggatgc accttgtagc ccgccgagat catcagcgac
    32101 cccattgaag actggtgcat ggtcggcagc agcgcaccga gcgcgatgat gaagaacatc
    32161 accttgttca gacgttgtag cgacaccttc cagcccagac gttcaaacag tgccggagca
    32221 aactccagag ccatcacgcc gatgtagatg gtcatacaga ccgccgtctc gaacagtacc
    32281 gagttcacgt tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg
    32341 tcgatagtga tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga
    32401 cgcaccagcg gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag
    32461 ccgccacagg caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg
    32521 aagccgccgt tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg
    32581 agcatacaga tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg
    32641 cccagcggtt gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt
    32701 gttgcgacga accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt
    32761 atacagggtg tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg
    32821 caggtcgaga ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc
    32881 tttctcgccg tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga
    32941 tttcagcgtc tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt
    33001 cgcctccgcc atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc
    33061 tacgcagcca ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc
    33121 gcacttatgc agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca
    33181 ggcgaccatg cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt
    33241 tttcggatct tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca
    33301 gtgcatacac tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc
    33361 tgtgccgctg gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt
    33421 cgaccaggtc tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt
    33481 gacgcaagcc tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg
    33541 aattggcggg cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa
    33601 tgccccgcag gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag
    33661 tcagcgttat ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg
    33721 gcaaccagcc caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc
    33781 tctttagcgt taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc
    33841 tgatggatgc ctttatggaa gccgatacct ttttcgttac agccatagca cgggtgacca
    33901 atcgccaccg gccacacacc gccaacatcg cagaattgca gcgttgagca gttgccgtaa
    33961 gtttctggcc ctttacagcc gaggtggtac aggcaccagc cttcgcggtg gccttcatca
    34021 ccgaactctt tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga
    34081 atcagacggc cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg
    34141 taagtgatga tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga
    34201 atattgataa cggttttgcc tggcagaact tcttgcaagc tgactgcgcc agttgggtta
    34261 actccagctg cggcaacacc gccccacgcg gagcaggaac caatagcgat aatggctgct
    34321 gagccttccg ccgctttgcg gatgtgatcc acaatcggct caccggcaac catgcaataa
    34381 ataccgttat cttttaatgg gatggaacca tccaccacta acacatactg ccctttgtac
    34441 ttctcgagag cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact
    34501 tcgtgatatt ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca
    34561 cgaagcagag attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga
    34621 cgctgcgggt tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg
    34681 gtggcggcta atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg
    34741 atgagggtgt tatctccagt catttatagt tattccgttg cgaggacctg gcatatattt
    34801 tgcctcaatc gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg
    34861 ttatcgatat catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa
    34921 ttaattaaag caagataata cgtatgtttg atcaattttc gaggcaataa ccggccataa
    34981 aaaagcgcaa cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat
    35041 ccagtccata gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg
    35101 tcttttcgta catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga
    35161 attttgtcac attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt
    35221 taccaaaatg gttattcatc gttcacctca ttcgttatat atcgccatat atagcgatat
    35281 tttaggcaaa gaacgacttt tgcgcacgct attttttcat acttgtttaa gacgtaatcc
    35341 cgtaagagat aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag
    35401 gcaagatcgg cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta
    35461 acccccggat agatataagc ctcataaacc ttattattgt ctttcaacgc cgcctcgtaa
    35521 ggaggccagc cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct
    35581 tcaatcttcg ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc
    35641 agttccggat acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta
    35701 atacccactt tacctgtcgc ttgcggatag cgttgcataa actcaattgc ggcaaagaaa
    35761 tcattcatca gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt
    35821 cccggataac ctccaacgga acttaatccg tcaggtgcca gggcgatata ccccgccttc
    35881 gccactcgcc gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc
    35941 cccacggctg gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg
    36001 ccgtgaccat ttggcgaagg atacgtgatg tactcagcaa caatctccgg gtcggtaaac
    36061 tctacctgag tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc
    36121 atcccgccca ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg
    36181 tgaacgtaat agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc
    36241 ggcatcgttg cattcctcaa ctggtgtttt tttaagcaaa gcataagcgc gtgtttttgc
    36301 ccagtttttc gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact
    36361 agaaacattg tttcgaaatt gaacggagga aaggagaggt catggtctgg ttagcgaatc
    36421 ccgaacgtta cgggcagatg cagtaccgct attgcggaaa aagtggttta cgcctgcccg
    36481 cattatcgct cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg
    36541 caatcctgcg taaagcgttt gatttaggca ttacgcactt tgatttagcc aacaattacg
    36601 ggccgcctcc aggaagcgca gaagagaact ttggtcgcct gctgcgcgag gattttgctg
    36661 cttatcgcga tgaactgatt atctctacca aggctggcta cgatatgtgg cccggcccgt
    36721 acggctctgg cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta
    36781 tggggcttga gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg
    36841 aagaaaccgc ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga
    36901 tctcctctta ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa
    36961 ttccgctttt aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg
    37021 gcctgctgga taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc
    37081 agggattgct gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg
    37141 aagggaataa agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc
    37201 tgcgcttatt gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa
    37261 gctggttgct gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc
    37321 aactagagga gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc
    37381 agattgatca gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat
    37441 gacctgttaa taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc
    37501 agtcggtcaa gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg
    37561 taccacatca gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga
    37621 atggaagaaa ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt
    37681 ttccccagcc acttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc
    37741 tcataaccgg aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc
    37801 gacagcaaca cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga
    37861 ataatctcct ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac
    37921 acgggggcaa gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat
    37981 tcctgtcgtg tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca
    38041 gcaaatcaac atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg
    38101 gttcccttat caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga
    38161 aaaacgatgt ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa
    38221 cagaaacaac cgacgccagg catccaggcg aagatgacac cgataccgga ttgcggcgag
    38281 aaaacctatg ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat
    38341 tccggaatag gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc
    38401 agttatcttc ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc
    38461 ggacgcaaag ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg
    38521 gttcacgaag cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa
    38581 caggttgcca ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc
    38641 attaacgttt tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt
    38701 gcaagtgtca tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac
    38761 tatgcggcta cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg
    38821 gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt
    38881 tccggcggac aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt
    38941 gcggggcaac cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc
    39001 tacgtcaccg cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc
    39061 cggttgtgaa aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac
    39121 gccttatccg cctacaaacg catgaagatt caacatgttg caatagattt tgtaggcctg
    39181 ataagcgtag cgcatcaggc attgagcacc taatgcctga tgcgtcgtga acgccttatc
    39241 cggcctacaa acgcacgaag attcaacatg ttgcaataga ttttgtaggc ctgatgagcg
    39301 tagcgcatca ggcaattcta ctttacttcg ctttggcggt ttcttcgccg accagaccta
    39361 tcttcaggta acccgcctga tgcagcgtat ccattacctt catcaacgtc tcgtaatcga
    39421 cggttttgtc cgctcggaag aagatggtgg tgtctttctt gccttcggtt aacgcattca
    39481 acgccgtaat cattgtttca tcggtgaccg gatcgttacc gataaacatc gagttgtctg
    39541 ccttcaccga cagataaacc ggtttttccg ggcgcggctg cggcgtgctg gtagaagcag
    39601 gcaagttcac cttcacatct accgtcgcta acggtgccgc caccataaag ataatcagca
    39661 gaaccaacat cacgtcgata aacggcgtca cgttgatatc atgcatttcg ccgttatcgt
    39721 cgaggttttc gttaagatgc attgccatcg gacattatcc tgcgcgtaat ttttgtgcga
    39781 cacgaaccgg atgcgcagcg gcgctggctt ccagatccag gtcacggctt tgcagcaaca
    39841 atacctgcgc tgcaacatca cccagcatcg ctttaaagcc gccaatctgg cgtgcaaata
    39901 cgttatagat aacgaccgca ggaatcgctg ccacgaggcc gattgccgtt gctaacagag
    39961 cttctgcgat acccggcgca acgactgcca ggttagtggt ctgcgtttgc gcgataccaa
    40021 taaagctgtt catgatgccc cagaccgtac caaacagacc aacgaacgga gaaatcgcgc
    40081 cgatggttgc cagatagccg ttaccgcggc ccatttgacg acccactgcg gcgacccgac
    40141 gctccagacg gaagctggta cgttctttaa taccttcgtt atcgtcgctg ccttctgaca
    40201 gttccagctc attctgcgct tcattgagca aatgcaggct taagcttttg ctaccaaaat
    40261 cagcggcgat atcgttggcc tggtttaagg aacgtgcttc agccagcagt tgctgctcgc
    40321 gcttaaggcg acgcttctga ttgaagaact ctacgctctt actgaagaag attgcccagg
    40381 tgactacaga ggccaaaata agcccaatca tcacgcactt aacgacaata tcggcgtact
    40441 gatacatacc ccagacggaa aggtccgtct gcattaaatt attacccact gtgtatctcc
    40501 aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa cgacgtcgaa ttgatagtcg
    40561 ttctcattac tatttgcata ctgccgtacc tttgctttct tttccttgcg tttacgcagt
    40621 aaaaaagtca ccagcacgcc atttgcgaaa attttctgct ttatgccaat tcttcaggat
    40681 gcgcccgcga atattcatgc tagtttagac atccagacgt ataaaaacag gaatcccgac
    40741 atggcggaca aaaagcttga tactcaactg gtgaatgcag gacgcagcaa aaaatacact
    40801 cttggcgcgg taaatagcgt gattcagcgc gcttcttcgc tggtatttga gagtatggaa
    40861 gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt tgttctatgg acggcgcgga
    40921 acgttaaccc atttttcctt acaacaagcg atgtgtgaac tggaaggtgg cgcaggctgc
    40981 gcgctatttc cctgcggggc ggcagcggtt gctaattcga ttcttgcttt tgtcgaacag
    41041 ggcgatcatg tgttgatgac caacaccgcc tatgaaccga gtcaggattt ctgtagcaaa
    41101 atcctcagca aactgggcgt aacgacatcg tggtttgatc cgctgattgg tgccgatatc
    41161 gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg aatcgccggg ttccatcact
    41221 atggaagtcc acgacgttcc ggcgattgtt gccgccgtgc gcagtgtggt gccggatgcc
    41281 atcattatga tcgacaacac ctgggcagcc ggtgtgctgt ttaaggcgct ggattttggc
    41341 atcgatgttt ctattcaggc cgccaccaaa tatctggttg ggcattcaga tgcgatgatt
    41401 ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac gggaaaatgc ctatctgatg
    41461 ggccagatgg tcgatgccga taccgcctat ataaccagcc gtggcctgcg cacattaggt
    41521 gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg ccgaatggct ggcagaacat
    41581 ccgcaagttg cgcgagttaa ccaccctgcc ctgcctggca gtaaaggaca cgaattctgg
    41641 aaacgagact ttacaggcag cagcgggcta ttttcctttg tgcttaagaa aaaactcaat
    41701 gatgaagagc tggcgaacta tctggataac ttcagtttat tcagcatggc ctactcgtgg
    41761 ggcgggtatg aatcgttgat cctggcaaat caaccagaac atatcgccgc cattcgccca
    41821 caaggcgaga tcgattttag cgggaccttg attcgcctgc atattggtct ggaagatgtc
    41881 gacgatctga ttgccgatct ggacgccggt tttgcacgga ttgtataaca ttgccacttt
    41941 tggacaattt tgcagacatt ttattgtgaa aagtcttaaa ttgttgcgtc cgggatcaag
    42001 gcgtcccgga cgattcagga gtacaatagg cagataaagg cttaaacgct gttccacagg
    42061 aaagtccatg gctgttattc aagatatcat cgctgcgctc tggcaacacg actttgccgc
    42121 gctggcggat cctcatattg ttagcgttgt ttactttgtc atgtttgcca cgctgttttt
    42181 agaaaacggc ctgctgcccg cctcattttt gccaggcgac agcttgttga tactggcagg
    42241 cgcattgatt gcccaagggg ttatggattt tctgcctacg attgcgattc tgaccgccgc
    42301 agcaagtctg ggctgctggc taagttatat tcaggggagc tggttaggga ataccaaaac
    42361 ggtgaaaggc tggctggcac agcttcctgc taaatatcac cagcgcgcca cctgcatgtt
    42421 tgaccgccac ggtctgctgg cgctgctggc tggacgtttt cttgcatttg tccgtacgct
    42481 gctgccaacc atggcgggaa tttccggtct gccaaaccgc cgcttccagt ttttcaactg
    42541 gttaagtgga ttgctgtggg tcagcgtggt aaccagtttt ggctatgcct taagtatgat
    42601 tccgttcgtt aaacgccatg aagatcaggt aatgacgttc ctgatgatcc tgccaattgc
    42661 cttgttaacc gctggcttgt taggcacgct gtttgtggtg attaaaaaaa aatactgtaa
    42721 cgcctgacga ttttccccgt tcccggctgc tgtaccggga acgtatttaa ttcccctgca
    42781 tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc gaagtaacgt ttaaactcac
    42841 ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc tgctgcgctg gccttcatgc
    42901 cgtcatggat gatcatcatc cgcgccttat gcagacggta attcttcaaa tactgcaacg
    42961 gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc cgatacgctc atgttggctt
    43021 ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt attctcaatt cgtttcagca
    43081 cgcggctaat cagactgaag tgagtctggc gactgaccag cgccagtaac gcgccgccgc
    43141 aaggtccggt cagcacgtag tacagaattt cgcggatgat ctgtttgccg agaatacgcg
    43201 catccagtgg tcgctccatc acgtcgagta accgctccgc cgcgcataaa atctcttctg
    43261 ataacgtggc ggagttaatc ccgctggctg ccatcgacgg ctggaaatgc tcatcttcgc
    43321 caatgtccat caacagttcc tgtaactgca aaatatcgac attgagacgc aaccctgcca
    43381 gcggcacctc tgacgtggca taggtttcgc actcaaacgg caacggcacc gtcagcagca
    43441 ggtattcatt ggcatcataa cgaaacacgc gttcattgat ataaccgatt ttatgcccgg
    43501 aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt acgtgcgaaa ggcgtctcgc
    43561 catacaacaa acgcacatcg ggcaacagtt ctgacaaact attttcttta tttttcagtt
    43621 tattaacttt atccgccagc aagcgacaaa tctcttcacg tttcatatcg cgtaatttct
    43681 taggaataat gcggcaattt gattgtgcgc aattttgtag catttctcca gcactctgga
    43741 gaaataggca agacattggc agaaatgagc attgagagcc agggcgctgg cgatcacaat
    43801 gaaaaacatc aggcagatcg ttctctgccc tcatattggc ccagcaaagg gagcaagtaa
    43861 tgaacaactt taatctgcac accccaaccc gcattctgtt tggtaaaggc gcaatcgctg
    43921 gtttacgcga acaaattcct cacgatgctc gcgtattgat tacctacggt ggcggcagcg
    43981 tgaaaaaaac cggcgttctc gatcaagttc tggatgccct gaaaggcatg gacgtgctgg
    44041 aatttggcgg tattgagcca aacccggctt atgaaacgct gatgaacgcc gtgaaactgg
    44101 ttcgcgaaca gaaagtgact ttcctgctgg cggttggcgg cggttctgta ctggacggca
    44161 ccaaatttat cgccgcagcg gctaactatc cggaaaatat cgatccgtgg cacattctgc
    44221 aaacgggcgg taaagagatt aaaagcgcca tcccgatggg ctgtgtgctg acgctgccag
    44281 caaccggttc agaatccaac gcaggcgcgg tgatctcccg taaaaccaca ggcgacaagc
    44341 aggcgttcca ttctgcacat gttcagccgg tatttgccgt gctcgatccg gtttatacct
    44401 acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga cgcctttgta cacaccgtgg
    44461 aacagtatgt taccaaaccg gttgatgcca aaattcagga ccgtttcgca gaaggcattt
    44521 tgctgacgct aatcgaagat ggtccgaaag ccctgaaaga gccagaaaac tacgatgtgc
    44581 gcgccaacgt catgtgggcg gcgactcagg cgctgaacgg tttgattggc actggcgtac
    44641 cgcaggactg ggcaacgcat atgctgggcc acgaactgac tgcgatgcac ggtctggatc
    44701 acgcgcaaac actggctatc gtcctgcctg cactgtggaa tgaaaaacgc gagaccaagc
    44761 gcgctaagct gctgcaatat gctgaacgcg tctggaacat cactgaaggt tccgacgatg
    44821 agcgtattga cgccgcgatt gccgcaaccc gcaatttctt tgagcaatta ggcgtgccga
    44881 cccacctctc cgactacggt ctggacggca gctccatccc ggctttgctg aaaaaactgg
    44941 aagagcacgg catgacccaa ctgggcgaaa atcatgacat tacgttggat gtcagccgcc
    45001 gtatatacga agccgcccgc taagcttttt acgcctcaaa ctttcgtttt cgggcatttc
    45061 gtccagactt aagttcacaa cacctcaccg gagcctgctc cggtgagttc atataaagga
    45121 ggaacgtatg gctaatccaa ccgttattaa gctacaggat ggcaatgtca tgccccagct
    45181 gggactgggc gtctggcaag caagtaatga ggaagtaatc accgccattc aaaaagcgtt
    45241 agaagtgggt tatcgctcga ttgataccgc cgcggcctac aagaacgaag aaggtgtcgg
    45301 caaagccctg aaaaatgcct cagtcaacag agaagaactg ttcatcacca ctaagctgtg
    45361 gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac agcctgaaaa aactccagct
    45421 tgattatatc gacctctact taatgcactg gcccgttccc gctatcgacc attatgtcga
    45481 agcatggaaa ggcatgatcg aattgcaaaa agagggatta atcaaaagca tcggcgtgtg
    45541 caacttccag atccatcacc tgcaacgcct gattgatgaa actggcgtga cgcctgtgat
    45601 aaaccagatc gaacttcatc cgctgatgca acaacgccag ctacacgcct ggaacgcgac
    45661 acacaaaatc cagaccgaat cctggagccc attagcgcaa ggagggaaag gcgttttcga
    45721 tcagaaagtc attcgcgatc tggcagataa atacggcaaa accccggcgc agattgttat
    45781 ccgctggcat ctggatagcg gcctggtggt gatcccgaaa tcggtcacac cttcacgtat
    45841 tgccgaaaac tttgatgtct gggatttccg tctcgacaaa gacgaactcg gcaaaattgc
    45901 aaaactcgat cagggcaagc gtctcggtcc cgatcctgac cagttcggcg gctaacgtgc
    45961 aaattctccc ggtggcggta atgttccgct accggacttt tcagaaatca tttattcccc
    46021 tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg ccaaatataa ggacatcatc
    46081 atgcagagcc ggaagctctt aaaagaacaa ctcatctata tccgggataa acgcaacgga
    46141 gaggtgaaaa acagatgaaa ataatacttc tgtttttagc agccctggca agttttaccg
    46201 tacacgcgca gcctccttca cagaccgtag aacaaacagt ccggcagatt tatcagaact
    46261 ataaatcaga tgccagtacg ccttatttcg gtgaaaccgg agagcgggcg ataacttcag
    46321 cgcgtattca acaggcgctt accctgaacg acaatcttac actaccgggc aatattggct
    46381 ggctggatta tgatccggtt tgtgattgtc aggattttgg cgatctggtg ttagaaagcg
    46441 tagcgataac ccaaactgac gtcgatcatg ccgatgccgt tgtgcgcttt cgtatcttta
    46501 aagatgataa agaaaagacc acacagacac tgaaaatggt ggcggaaaat ggtcgttggg
    46561 tcattgatga tattgtcagc aatcatggca gcgtcttaca agcagttaat agcgagaatg
    46621 aaaaaacgct ggccgcttta gcttcattgc agaaagaaca gccagaatcc tttgttgctg
    46681 aactctttga acatattgct gattatagct ggccgtggac gtgggtggtt tccgactctt
    46741 accgccaggc ggttaatgcc ttctataaaa ccaccttcaa gacggccaat aatcccgatg
    46801 aagatatgca aatagaacgg caatttattt acgacaatcc gatctgtttt ggcgaagagt
    46861 cgctattttc acgcgttgat gaaattcgag tcctggagaa aaccgccgat tccgcccgca
    46921 ttcatgttcg ttttacgctg accaatggca acaatgaaga gcaagagctg gttttacagc
    46981 ggcgcgaagg taagtgggaa atcgctgatt ttatccgccc gaacagcggc agcctactta
    47041 agcagattga ggcaaaaacc gccgccagat taaagcaatg agctgaatta aataacaatt
    47101 agccggaaca ataaataaaa gggaacacta tatgaaaacg attttcaccg tgggagctgt
    47161 tgttctggca acctgcttgc tcagtggctg cgtcaatgag caaaaggtca atcaactggc
    47221 gagcaatgta caaacattaa atgccaaaat cgcccggctt gagcaggata tgaaagcact
    47281 acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc gtcttgatgc
    47341 acaggactat tttgattgcc tgcgctgctt gcgtatgtac gcagaatgat aaaaaaatcc
    47401 ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct ttagggcgtt
    47461 tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag
    47521 gcgtctgacg ctgagtcgcc atcggcgtat gtttagtcaa cgccggacgg gtattgcggt
    47581 tctggcgacg agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac
    47641 ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctgacggatt aacggccagt
    47701 ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg tcgcccttcg
    47761 gtacgaagac gccttcgctc ttataaccaa tcttcgccag cggttttttc ccggtgtaat
    47821 acatggtggt tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga
    47881 aacgatgctt tttcagccac agcgccaaat tcaccatatc ttcatcacgc gtaccggggt
    47941 gcgcagagat gaaatacggg atcagatact gctctttacc tgcctgtttc gagtaggtat
    48001 cgaacagctc tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgacaacg
    48061 gcccttcttc ggtatgttcc ggagcaatct tcagataacc gccgacgtga tgggtcgcca
    48121 actctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct gaggcgataa
    48181 ggattttttt aatgcctttc agatcacgcg cacggcgata gaggttgatc gtcggttcgt
    48241 ggttagtgtc catgtgcgga caaatatccg gataaacgca cgacaaacgg cgacaagttt
    48301 gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat
    48361 cggaaatcac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga
    48421 tcgaatcttc cgaacggctc tgaataatgc gcccttcgtg ctcggtaata gaacagaaag
    48481 aacagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg
    48541 gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc gcaaaaacgc
    48601 tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca tagcggtcac
    48661 cgtgtttttg catcaatgcg cgggcgcagc ctgggttggt ttcgtggtgc agaatgcgcg
    48721 aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaatacgt
    48781 aggttttttc ccacggtttc tggcgcggtg gctgcacgat tacagctttc gcttcctgct
    48841 ttttcggcgc aacaggtttg ttatccgcac acggcaaatc ttcgccatac ggatgcggga
    48901 ttggggcgat tttccctggg gtatcaagac gggtggaatc cacaccgctc cagccaggca
    48961 gcgcctcttt tacgataatt gcggtattac gcacatcgcg gatttcacta atcggctcgc
    49021 ccatcgccag acgatgcact acttccacca gcggacgttc accgttacca aacatcagca
    49081 tgtcggcttt cgaatctacc agaacggaac ggcgcacggt atcggaccag taatcataat
    49141 gcgcggtacg gcgcaggcta gcctcaatac cgccgaggat caccggcaca tctttccacg
    49201 cctctttaca acgctgggta taaaccagcg tggcgcgatc cggtcgctta cccgcgacat
    49261 tatctggcgt gtaggcatca tcatgacgta aacggcgatc ggcggtatag cggttgatca
    49321 tcgaatccat gttgccagca gtaacaccaa aaaacagatt cggtttaccc agacgcataa
    49381 agtcgtcttt gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt
    49441 ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag
    49501 taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca cgagacatcg
    49561 gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc
    49621 gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat
    49681 caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaggtattc catactcgcc
    49741 ctcctcgggc gagtatgaag attacggtac cggattgacc aacagttgcc caatcgagcc
    49801 acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg
    49861 ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc
    49921 tctgtcttcc gggaacggca tcgcgccatt cacgttacgg atctggaaca ttacgccttc
    49981 aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg
    50041 cgcggtgaca tcaatacggt tgacgtccca cagctgtcca ttaatacccg ggtcatcacc
    50101 cagactgata tcgcgactgc gaattggcga accagccatg atttcagttg gcagcaagcg
    50161 catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccaa
    50221 ggtagaaacc agaatactgg atggctcaaa gaagccgcga atacgatcaa caatgctcgc
    50281 cgcttcacca caggtgatcg acacttcatc gccgttgctc atatccacca gaatctcgcg
    50341 gcgctcgccc ggtgccagcg aaagttgctt caccgataca ggagcaggca ggaatccctg
    50401 atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat agcgacgaga
    50461 gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct
    50521 ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta
    50581 ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac
    50641 accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc
    50701 ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag
    50761 agtagctgcg ttctggcgaa taggcagtac gggtgcccag tcagcgtttg gcgacatcat
    50821 ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac
    50881 attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat
    50941 agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa
    51001 tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag
    51061 cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc
    51121 tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc
    51181 tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga
    51241 ttgccgactt aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg
    51301 attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca
    51361 tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga
    51421 ttaagattaa tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc
    51481 gcaattgccg cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg
    51541 gttccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc
    51601 gcaatggtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac
    51661 cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc
    51721 ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata
    51781 tagatagcat tgccgtagtt ttcagcatct gcaggtttgc ggcactcaac tttcaggccg
    51841 aacagtggcg caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc
    51901 gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta
    51961 ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga ctggcattgt
    52021 acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
    52081 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg
    52141 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgctag cgggaggaaa
    52201 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
    52261 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
    52321 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
    52381 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc cagaccatcc tcgccacgcg
    52441 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
    52501 gatcacttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
    52561 aagcatcttc aatcaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
    52621 ccttacccgc acggttacgc gctaccagat cgttaaaggt gcagacgaaa ccgtaacccg
    52681 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
    52741 ccccaggcgg caacgttaat ttgccggtga gtggttcgcc ctgaccacgc gccgacggca
    52801 gcgtaatcgg gtcgatggca tagctacgac cggtggaatc aacaaacact accggttggt
    52861 tactcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
    52921 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
    52981 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca
    53041 acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca
    53101 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
    53161 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
    53221 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
    53281 tcggttcatc ttcgttacga atgatctcaa tcacttcgtc gatgttgaga aacgccacca
    53341 gcaaaccttc caggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc
    53401 ggcgcacggt atcgcgacgg aacaccagcc attcggagag gatttccagc aggtttttca
    53461 ccgccggacg accatccaga ccgatcatat tgaggttaat gcgatagctc ttttccagat
    53521 cggtggtagc gaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
    53581 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
    53641 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
    53701 gatgcggtaa tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac
    53761 gcactgaacc acgtccgttc tcgtagattt tacgaatctc ggcgcgcgaa gtgataattt
    53821 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtagttt
    53881 tcggctggtc gattaatgcg attgccgcct gagctacttc acgcaggtta tgcggtggaa
    53941 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
    54001 gggcaggtag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
    54061 tcccctgccc cagctcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
    54121 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc tgaccatcaa
    54181 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcgc
    54241 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
    54301 atttggcgct ggcattcagg cccagttcag acatcgcata cacaatgcgg cgctgaacag
    54361 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
    54421 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
    54481 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
    54541 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
    54601 acggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cttgatcggc
    54661 atagagggca gcacaaggtt gcgcccatgc cacaccactc ggcatggggg tccgctccgg
    54721 gcgattcgag aagttgaggt tatgcgagtc cagggaagcc taaagcatca aagtagtcga
    54781 ctgtaagtat acggttcagc ccagtttagc tgtgtctcca ccagtgcccc gcgcctcctg
    54841 ccataagtgc ggcttgctca tggatatccc cgagacggct tacggcgcac cggataataa
    54901 atttcgcgta tacatggtat tccttaccgg agaaattata agcccaacaa caatacgcct
    54961 gtgacattgt tcgctcaaac ttatcgcgag tgatttggct cacactccgg taaagattcc
    55021 ccatcacgcc aacggcacat aaacatccgt ttccagtcgc tgctctgtca ccccttcggc
    55081 aagattggtg tagtgaaaca gaatcggtgc tttacgcatt ttctcgccgc ttgcaggcag
    55141 ccagtggcga ataatgcccc atatcgtgtg tgaaatatcg tccagctcgc caacgtggcg
    55201 ggccacggca taacgtccac cggtaagttc accattgctc acaccataac gattatcggg
    55261 aatcggttcg ctaacgctgc cgcagatatc aaagcgaaac gcttccggtg cggtggtggc
    55321 aggggcgtcc caggcgacgc caaaagtctg gctttggtta acaggagaaa gccccgtctc
    55381 tttacgccac atgataaatt tcgccacact gtagttgagc aactcagggc tacagcggtg
    55441 cggcaacatc gccacaggga ttgatgggaa atcgataatg tttacatcca gtgtcaggtt
    55501 tgtcattttg cctccctgcg aaattattcc cgggaacgcg tcccgggagc ggtaacaatt
    55561 acggatttac tttgcggatc tgtttgacgt caatttcaac agaattccag tctttatcga
    55621 cttcaccctg aatctcaacc gtatctttcg gcgtcaccgt cacaccgttc cagcgtttgt
    55681 gatcgatatc aacattgata gtaccgctgg catctttaaa cacgtagaga tcgtcagaga
    55741 tgcgttcaac gatattgccg cgcagggtta cccaggtgtc gtcacgcagg gattttgcgc
    55801 tttctacagt cgttacgctg ccgttcggcc cctgaaatcc tccggcctga ctttgcgtta
    55861 ccgatgggcc agaaaaaccg ccctgctctg ctgccatcac cggtgcactg cacagggcca
    55921 ttactgcgat tactgctgcg aattttttca tgtttattac tccctttaat gtctgtttcc
    55981 gagcatttaa caagatagtc cttaacaact tcttaaggga aaaaaataaa atttaatgct
    56041 gtacagagcg cgttacaaca cggtttactg gcagcaaata cggttatcgc agggatgaaa
    56101 aaatgcgaat tttactgata gaagatgaca tgctgattgg tgacggcatc aaaactggcc
    56161 ttagtaaaat gggttttagt gtcgactggt ttacacaagg tcgtcaggga aaagaggcgc
    56221 tttatagcgc accttatgat gcggtgatcc tggatttaac cttaccaggc atggatggtc
    56281 gcgatatttt gcgcgaatgg cgagagaaag gtcagcgtga gccggtactg aacctgaccg
    56341 cgcgtgatgc gttagcggaa cgtgtagaag ggctgcgtct gggagctgac gattatctgt
    56401 gtaaaccttt tgcgttgata gaagtcgccg ccaagctgga ggctctgatg cgccgaacca
    56461 acggccaggc cagcaacgag ctgcgccacg gtaacgtcat gctcgacccc ggcaaacgta
    56521 tcgccacgct ggctggcgaa cccttaacac tgaaaccaaa agaatttgcc ctgctggaat
    56581 tactgatgcg taacgctggt cgggtactgc cgcgcaaact gattgaagag aaactgtata
    56641 cctgggacga agaggtcacc agtaatgccg ttgaagtgca tgtgcatcat ctgcgacgca
    56701 aactcggtag tgattttatt cgtaccgtgc atggtattgg ctacacatta ggtgagaaat
    56761 gaaatttacc caacgtctta gtctgcgcgt caggctgacg ctaatctttt taattctggc
    56821 ctcggtgacc tggctgcttt ccagctttgt cgcctggaaa caaacaacgg ataacgtcga
    56881 tgaattgttc gacacccaac tgatgctgtt tgccaaacgg ttaagtacgc tcgatctcaa
    56941 cgaaatcaac gcggcggatc gcatggcaca gacgccaaat aaattaaaac acggtcatgt
    57001 tgatgacgat gcgctgacct ttgccatctt tacccacgac ggcagaatgg tccttaatga
    57061 tggcgataac ggagaagata ttccctatag ctatcaacgg gaaggttttg ctgacgggca
    57121 actggtcggt gaagacgatc cttggcgttt tgtctggatg acctcacctg atggcaaata
    57181 tcgcatcgtt gttggccagg aatgggaata ccgtgaagac atggcgctgg cgattgttgc
    57241 cgggcaattg atcccgtggc tggtcgcact gccgattatg ttaatcatca tgatggtact
    57301 actgggtcgt gaactcgcgc cgctgaacaa actggcgctg gcactacgta tgcgtgaccc
    57361 tgactcggaa aaaccactaa acgcgactgg cgtacccagc gaagtgcggc cactggttga
    57421 gtcgctaaat caactgttcg cccgcacaca tgcgatgatg gttcgagaac gacgctttac
    57481 ctccgatgca gctcacgaac tacgtagccc gttaacagcg ctgaaagtgc aaaccgaagt
    57541 tgcgcagctc tctgacgatg atccgcaggc gcggaaaaaa gcactgctcc aattacattc
    57601 cgggatcgat cgcgctactc gtctggttga tcaactgctc acgctatcgc ggctggactc
    57661 actggataac cttcaggacg tcgcggagat cccgcttgaa gatctcctac aatcgtcggt
    57721 gatggatatt taccacacgg cgcagcaggc gaaaattgac gtgcgactga cactcaatgc
    57781 ccacggcatc aaacgcaccg gacaaccgct attgctaagt ttgttggtgc gaaatttgct
    57841 ggataacggc cccggtgtga caccagaggc actggcgcga attggcgaac gcttctatcg
    57901 cccacccgga caaaccgcta ccggcagtgg acttgggcta tcgattgtcc agcgaatcgc
    57961 caagctgcat gacatgaatg ttgagtttgg gaatgcggaa caaggtggat ttgaggcgaa
    58021 ggtaagctgg taacttctct gtctatgaga gccgttaaaa cgactctcat agattttact
    58081 aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc acaaaccaga caaataacga
    58141 gcgagaatac atatagccaa aactcgaaca tttcccgcat aaagagtttt cttaagataa
    58201 gaataataag tggcgtaaga agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt
    58261 cgcctgaaac ggaaggtata ctgcaaatat tgcctcccga ctcaagcatc cagtcatact
    58321 tattgaaagc aaaaaaagaa tctattaacc aataagtaag cattatatat agcaatgctt
    58381 caaatattgt tttaattttc tgctttaaca tatatcagta cgctcataaa aatccattta
    58441 taattatcaa catgacacgt tgatattttc atcttttact tattcaaagc catattttca
    58501 atccccaaaa aaaccgaacc aaacgtgtaa attattccgg tgcgagtaag aattatgagg
    58561 aatggctatc agtattgtca ttttcagaaa atatttatcc tgcatcggtg agtcagagct
    58621 agatcagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcacagtacc
    58681 gctctgtgac gtactatagt cgacaaacgt ctcaccttga gattaaaaaa tgagcaacat
    58741 cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct
    58801 gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc
    58861 cgacagcgac tacaatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat
    58921 ctggcagatg ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga
    58981 tgtattcacc gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc
    59041 gtcgaaaaaa tacggttccg gcagcctggt acagggcaaa aaatatatgc tttctctaac
    59101 ctggaacgca ccaatggaag ccttcaccga aaaagatcag ttcttccacg gcgttggcgt
    59161 tgacggtgtg tatctgccgt tccataaagc aaaccaattc ctcggtatgg aaccgctgcc
    59221 gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata
    59281 tcgcaagcat cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac
    59341 catgcttacc gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt
    59401 attggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta
    59461 tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat
    59521 cgtgatgatt gagcagtggg aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca
    59581 catgaaggcg tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata tccgtattct
    59641 gcagccaggg atttaatcct gccttgtttg cccggccatc ctgaccgggc aatgttcttt
    59701 cctttaaacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc
    59761 cgagcgtttc ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc
    59821 atcgatagtc aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg
    59881 catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg
    59941 ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt
    60001 cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa
    60061 cgcgcggaag tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga
    60121 gtccgcatcc gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtc
    60181 aggatcgata ccgatcgcta ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc
    60241 ggaagaaact tcccaggtgt taaggatctt acctttcagc ggcatgatcg cctgatattc
    60301 acgatcgcgc gcctgcttgg cagatccgcc tgcggagtca ccttccacta ggaacagttc
    60361 ggtacggtta aggccctgcg cggtacaatc agccagtttg ccaggcagcg ccgggccgct
    60421 ggtcagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat
    60481 cgccatctct gccagcaact ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc
    60541 atctttcacc acgcccgaaa cgaatgccgc acactgacgc gaagagagac gctctttggt
    60601 ctgcccggca aactgcggat cctgcatttt aaccgacaga acataggcgc agcgatccca
    60661 gatatcttcc gccgacagct tcacgccgcg cggcagaatg ttgcggtatt cacagaactc
    60721 acgcatcgcg tccaacaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt
    60781 tgggataagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag
    60841 cgcccagtcc acagcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag
    60901 cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca
    60961 gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac
    61021 tgctttggcc ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa
    61081 ggtttcatcc ggccagaagt gcacgctggt accggtattg cgtttaccgc aagtgccgac
    61141 aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc
    61201 gcggcgcacg ttaacttcta cgcgcttcga caaggcgtta actaccgaaa tccccacgcc
    61261 atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg
    61321 gcaaagaatc agttcaaccg ccggtactcc ttcttccggg tgaatatcca ccggcatccc
    61381 gcgcccatcg tcaatcactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt
    61441 tgcgtgaccc gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt
    61501 agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag
    61561 tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg
    61621 atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc
    61681 ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca
    61741 ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg
    61801 gcgcttccag cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca
    61861 catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcaattcaa
    61921 acgggcgtac cgccgggttt accaccactg cgggcagcat aaaacattgc gacaaccagg
    61981 tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca
    62041 ggacaatgga ttccagcagt tctgccgcgt cggaaggata cggcggcaac tgcggaatga
    62101 tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag
    62161 agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga
    62221 agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc
    62281 atcagcatgt aactcgagag tacgccagcc gggcgcgata gtatccagcg taaagttgga
    62341 acagtgcggc ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca
    62401 gtcgagatcc agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt
    62461 cgccagcacg gtatccagtt cgcccgcgtt acgcagactg tgttgatcga gccaactaca
    62521 acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc
    62581 cagtttacgt tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa
    62641 cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg
    62701 ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg
    62761 cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc
    62821 agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg
    62881 tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc
    62941 ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatccttc ctctggcctc
    63001 accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta
    63061 gtaaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa
    63121 ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg
    63181 cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata
    63241 cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat
    63301 tgtcactagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc
    63361 cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg
    63421 tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat
    63481 ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac
    63541 cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac
    63601 cacatgaacg cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt
    63661 ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag
    63721 gaaacttaac accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg
    63781 acgggcaaca tcttccacac tttcaccctc ttcaatcatc ccggcaacca tctccagtag
    63841 ccagggggtt tcgctggtgt cgtacgcggc aatccgaatc tgctcaatca gcacaacttc
    63901 atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg
    63961 ccgtacctca tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa
    64021 tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa
    64081 tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctagcactaa tagtgaatta
    64141 aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catatgttaa attgaggcac
    64201 attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg
    64261 caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag
    64321 aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc
    64381 gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct
    64441 gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca
    64501 cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc
    64561 aactcgaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa
    64621 tggcgtgcgt taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc
    64681 gatcagcaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt
    64741 gacgttcttt cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc
    64801 acccaacgtt ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag
    64861 tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag
    64921 cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac
    64981 tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac
    65041 ctgtcgctgt tacaggcacg cttgagccag gacctggagc gcgagcaaat tcgccaggcg
    65101 caggatggtc acttaccgac tctggattta acggcttcta ccgggatttc tgacacctct
    65161 tatagcggtt cgaaaacccg tggtgccgct ggtacccagt atgacgatag caatatgggc
    65221 cagaacaaag ttggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg
    65281 caggtgaaac aggcacagta caactttgtt ggtgccagtg agcaactgga aagcgcacat
    65341 cgtagcgtcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc tatcagtagt
    65401 attaacgcct acaaacaagc cgtagtttcc gcgcaaagct cattagacgc gatggaagcg
    65461 ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcgaccac cacgctgtac
    65521 aacgccaagc aagagctggc gaatgcgcgt tataactacc tgattaatca gctgaatatt
    65581 aagtcagccc tgggtacgtt gaacgagcag gatttgctgg cactgaacaa tgcgctgagc
    65641 aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt
    65701 gctgatggtt atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact
    65761 accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc
    65821 gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc
    65881 ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc ccggaagaca
    65941 aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc
    66001 catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag
    66061 agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc
    66121 aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg
    66181 ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgtcagcaa
    66241 gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggctcagca atccagcggg
    66301 agtttctgga tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg
    66361 ggatttgcac agcagccgct gttctcctcg aaaaacccgg ccagtccggc ttacggtaaa
    66421 tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta
    66481 ccgaagacgg caatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt
    66541 ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caaccggcac ctcttctcgt
    66601 tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg
    66661 agaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg
    66721 aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg
    66781 aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga
    66841 ccaaattccg cataccaaaa cacacctgga gttttgtgcg tcaatcatgg ctgactcacc
    66901 agccatcgct ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaacttc
    66961 tggaaaataa cgccgatacg ccaacgtcac tatacgaggc ggcgttcttt cagtggatct
    67021 ggctggaaga tcagcttaac gccggtaact tgtcggaggg cagcgaccag tttaacagtc
    67081 tgcaagaaaa actgatcgat cgcttcgttg agctgcgtga acagtatggc ttccagttgc
    67141 tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg
    67201 actgcgcaac cgaagctgaa atcgccaccg agttcctcta catcgacgag ataggtttag
    67261 gcgaaaaagg ccagttcacg gatttacagg atcaggtgat ttctaacctg ttcaagcttt
    67321 atccgtggga atttatgctg cgcgaaatgt tctccaccaa gctggaagat gctggcgtac
    67381 gctggctgga accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt
    67441 gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc
    67501 cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga
    67561 tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga
    67621 ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct
    67681 ggctggtgaa cgatcaacct gccggaattg gcattcgtga agaccgtgcg ttgatcaccc
    67741 aggatatgtc tcggttttat ccacatattt ttgttgaata agccacgata ccggatggca
    67801 ctcgccatcc ggtaattgtt agcctatctg caccgacagc atacttaaac tgcccatttc
    67861 tataccatcg accggaatgg taattggctc ctgcccatcc cacacaccta acacatacaa
    67921 caacggcaga tagtgctctg gcgttgggtt cgataacgcg ccaccttcat ggtcgagata
    67981 attcaccaga ggatgctgct ctaccggccc ttgccacgtc agattcgctt tcacatactc
    68041 attaaacgac gtcgcccacg gatacggtga actatcaccg tgccacttca ctgtgcgcag
    68101 gttatgcacc acgttaccgc tggcgaccaa cattattcct tcatcacgca gcgccgcgag
    68161 tttgcgcccc atttcgaaat gccaggcgac aggtttgcta ctgtcgatac tcaactgcac
    68221 catagggata tcggcgtcag gatacatctt aatcagcacg ccccacgagc cgtggtcaaa
    68281 gccccaggct tctttatcca gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg
    68341 tgccagcgca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc
    68401 accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa
    68461 ccagtgagcc gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg
    68521 ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa
    68581 caatgctggc atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat
    68641 atcctcacaa tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc
    68701 agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc
    68761 tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct
    68821 cggacaaaaa ccctcgaacc gcttactggc gttttcgcta ggttttgcgg cggggatcat
    68881 gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc
    68941 ggtgttgggt tatggaatgt ttatcttcgg tctgcttggc tactttggcc tggaccgcat
    69001 gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg gtgcagctgt tgccaaaatc
    69061 gatcaagcgc acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg
    69121 gattgccacc tttgtcacgg cgagcagcaa cctggagctg ggatttggca tcgcactggc
    69181 cgtcgcgttg cacaatatcc ctgaaggtct ggcagtggca ggcccggttt atgcggcaac
    69241 ggggtctaaa cgcaccgcga ttctgtgggc ggggatttct ggactagcag aaattcttgg
    69301 tggtgtgctg gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat
    69361 catggcggcg gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc
    69421 caaagagatt gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat
    69481 gggattcagt ttagtgctgc tacaaacggc gggaattggt taaaaacaac atcagaagtt
    69541 gtccagcaca tggacaactt ctgccagctt cacgaaagcc ttattctacc ttttcggctg
    69601 tctcttctct cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct
    69661 ggcgaaacgc ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagagcaa
    69721 tcaatacaat ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta
    69781 aacatttagc gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat
    69841 aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt
    69901 ttctcataga aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa
    69961 gccagctaca acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc
    70021 tttacgctca tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt
    70081 gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt
    70141 cagctcacac agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc
    70201 ttcagtatga ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg
    70261 gcccgggcga ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt
    70321 aatacgatca gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt
    70381 aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt
    70441 acggcgatct tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc
    70501 catctgctca acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc
    70561 ttcatcatca agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac
    70621 acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca
    70681 ctaaaattat ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata
    70741 acgtgagcgg gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc
    70801 ggccccggaa ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc
    70861 tttcaactga gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga
    70921 ttcactataa cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat
    70981 gacttaccct tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat
    71041 gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg
    71101 aatcagggag ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct
    71161 gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg
    71221 gactcgtgag aaactggcgc tgcttgaaca aagaatcagc gaactggaaa atcgcagtac
    71281 tgagatcaaa aagcagccag atccagagac tctcccccca acgctgtaaa ca
//
LOCUS       DAJTUN010000005        59820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTUN010000005 DAJTUN010000000
VERSION     DAJTUN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.005"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..165
                     /locus_tag="OO470_000302"
     CDS             <1..165
                     /locus_tag="OO470_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCR6249682.1"
                     /translation="DPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFK
                     ATSTFQVTYP"
     gene            278..1072
                     /gene="panB"
                     /locus_tag="OO470_000303"
     CDS             278..1072
                     /gene="panB"
                     /locus_tag="OO470_000303"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6249683.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1084..1935
                     /gene="panC"
                     /locus_tag="OO470_000304"
     CDS             1084..1935
                     /gene="panC"
                     /locus_tag="OO470_000304"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6249684.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2009..2911)
                     /gene="rpnC"
                     /locus_tag="OO470_000305"
     CDS             complement(2009..2911)
                     /gene="rpnC"
                     /locus_tag="OO470_000305"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6249685.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3185..3565
                     /gene="panD"
                     /locus_tag="OO470_000306"
     CDS             3185..3565
                     /gene="panD"
                     /locus_tag="OO470_000306"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6249686.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3569..4798)
                     /locus_tag="OO470_000307"
     CDS             complement(3569..4798)
                     /locus_tag="OO470_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6249687.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4862..5302)
                     /locus_tag="OO470_000308"
     CDS             complement(4862..5302)
                     /locus_tag="OO470_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6249688.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5407..6177)
                     /locus_tag="OO470_000309"
     CDS             complement(5407..6177)
                     /locus_tag="OO470_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6249689.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6174..7100)
                     /locus_tag="OO470_000310"
     CDS             complement(6174..7100)
                     /locus_tag="OO470_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6249690.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVEHKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7209..7871
                     /gene="can"
                     /locus_tag="OO470_000311"
     CDS             7209..7871
                     /gene="can"
                     /locus_tag="OO470_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6249691.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7912..8448)
                     /gene="hpt"
                     /locus_tag="OO470_000312"
     CDS             complement(7912..8448)
                     /gene="hpt"
                     /locus_tag="OO470_000312"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6249692.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8654..11044
                     /gene="gcd"
                     /locus_tag="OO470_000313"
     CDS             8654..11044
                     /gene="gcd"
                     /locus_tag="OO470_000313"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6249693.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11091..12641)
                     /gene="cueO"
                     /locus_tag="OO470_000314"
     CDS             complement(11091..12641)
                     /gene="cueO"
                     /locus_tag="OO470_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6249694.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12807..13154
                     /locus_tag="OO470_000315"
     CDS             12807..13154
                     /locus_tag="OO470_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6249695.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13260..14126
                     /gene="speE"
                     /locus_tag="OO470_000316"
     CDS             13260..14126
                     /gene="speE"
                     /locus_tag="OO470_000316"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6249696.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14142..14936
                     /gene="speD"
                     /locus_tag="OO470_000317"
     CDS             14142..14936
                     /gene="speD"
                     /locus_tag="OO470_000317"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6249697.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14974..15336)
                     /locus_tag="OO470_000318"
     CDS             complement(14974..15336)
                     /locus_tag="OO470_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6249698.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15511..18108)
                     /gene="acnB"
                     /locus_tag="OO470_000319"
     CDS             complement(15511..18108)
                     /gene="acnB"
                     /locus_tag="OO470_000319"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6249699.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18463..20316
                     /locus_tag="OO470_000320"
                     /pseudo
     CDS             18463..20316
                     /locus_tag="OO470_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20387..21811)
                     /gene="lpdA"
                     /locus_tag="OO470_000321"
     CDS             complement(20387..21811)
                     /gene="lpdA"
                     /locus_tag="OO470_000321"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6249700.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22136..24016)
                     /gene="aceF"
                     /locus_tag="OO470_000322"
     CDS             complement(22136..24016)
                     /gene="aceF"
                     /locus_tag="OO470_000322"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6249701.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24031..26694)
                     /gene="aceE"
                     /locus_tag="OO470_000323"
     CDS             complement(24031..26694)
                     /gene="aceE"
                     /locus_tag="OO470_000323"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6249702.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26855..27619)
                     /gene="pdhR"
                     /locus_tag="OO470_000324"
     CDS             complement(26855..27619)
                     /gene="pdhR"
                     /locus_tag="OO470_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6249703.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28160..29533
                     /gene="aroP"
                     /locus_tag="OO470_000325"
     CDS             28160..29533
                     /gene="aroP"
                     /locus_tag="OO470_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6249704.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMAPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29576..30430)
                     /gene="ampE"
                     /locus_tag="OO470_000326"
     CDS             complement(29576..30430)
                     /gene="ampE"
                     /locus_tag="OO470_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6249705.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30427..30978)
                     /gene="ampD"
                     /locus_tag="OO470_000327"
     CDS             complement(30427..30978)
                     /gene="ampD"
                     /locus_tag="OO470_000327"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6249706.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31066..31959
                     /gene="nadC"
                     /locus_tag="OO470_000328"
     CDS             31066..31959
                     /gene="nadC"
                     /locus_tag="OO470_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6249707.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32162..32602
                     /gene="ppdD"
                     /locus_tag="OO470_000329"
     CDS             32162..32602
                     /gene="ppdD"
                     /locus_tag="OO470_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6249708.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32612..33759
                     /gene="tadA"
                     /locus_tag="OO470_000330"
                     /pseudo
     CDS             32612..33759
                     /gene="tadA"
                     /locus_tag="OO470_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33749..34951
                     /gene="hofC"
                     /locus_tag="OO470_000331"
     CDS             33749..34951
                     /gene="hofC"
                     /locus_tag="OO470_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6249709.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(34986..36029)
                     /gene="guaC"
                     /locus_tag="OO470_000332"
     CDS             complement(34986..36029)
                     /gene="guaC"
                     /locus_tag="OO470_000332"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6249710.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36191..36229)
                     /locus_tag="OO470_000333"
     CDS             complement(36191..36229)
                     /locus_tag="OO470_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249711.1"
                     /translation="MIIKTLKMSARK"
     gene            36254..36874
                     /gene="coaE"
                     /locus_tag="OO470_000334"
     CDS             36254..36874
                     /gene="coaE"
                     /locus_tag="OO470_000334"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6249712.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36874..37617
                     /gene="zapD"
                     /locus_tag="OO470_000335"
     CDS             36874..37617
                     /gene="zapD"
                     /locus_tag="OO470_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6249713.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37627..37824
                     /gene="yacG"
                     /locus_tag="OO470_000336"
     CDS             37627..37824
                     /gene="yacG"
                     /locus_tag="OO470_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6249714.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(37924..38313)
                     /gene="mutT"
                     /locus_tag="OO470_000337"
     CDS             complement(37924..38313)
                     /gene="mutT"
                     /locus_tag="OO470_000337"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6249715.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38373..41078)
                     /gene="secA"
                     /locus_tag="OO470_000338"
     CDS             complement(38373..41078)
                     /gene="secA"
                     /locus_tag="OO470_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6249716.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41140..41652)
                     /gene="secM"
                     /locus_tag="OO470_000339"
     CDS             complement(41140..41652)
                     /gene="secM"
                     /locus_tag="OO470_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6249717.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(41883..42800)
                     /gene="lpxC"
                     /locus_tag="OO470_000340"
     CDS             complement(41883..42800)
                     /gene="lpxC"
                     /locus_tag="OO470_000340"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6249718.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(42901..44052)
                     /gene="ftsZ"
                     /locus_tag="OO470_000341"
     CDS             complement(42901..44052)
                     /gene="ftsZ"
                     /locus_tag="OO470_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6249719.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44113..45375)
                     /gene="ftsA"
                     /locus_tag="OO470_000342"
     CDS             complement(44113..45375)
                     /gene="ftsA"
                     /locus_tag="OO470_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6249720.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45372..46202)
                     /gene="ftsQ"
                     /locus_tag="OO470_000343"
     CDS             complement(45372..46202)
                     /gene="ftsQ"
                     /locus_tag="OO470_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6249721.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46204..47124)
                     /gene="ddlB"
                     /locus_tag="OO470_000344"
     CDS             complement(46204..47124)
                     /gene="ddlB"
                     /locus_tag="OO470_000344"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6249722.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47117..48592)
                     /gene="murC"
                     /locus_tag="OO470_000345"
     CDS             complement(47117..48592)
                     /gene="murC"
                     /locus_tag="OO470_000345"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6249723.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48646..49713)
                     /gene="murG"
                     /locus_tag="OO470_000346"
     CDS             complement(48646..49713)
                     /gene="murG"
                     /locus_tag="OO470_000346"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6249724.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49710..50954)
                     /gene="ftsW"
                     /locus_tag="OO470_000347"
     CDS             complement(49710..50954)
                     /gene="ftsW"
                     /locus_tag="OO470_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6249725.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(50954..52270)
                     /gene="murD"
                     /locus_tag="OO470_000348"
     CDS             complement(50954..52270)
                     /gene="murD"
                     /locus_tag="OO470_000348"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6249726.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52273..53355)
                     /gene="mraY"
                     /locus_tag="OO470_000349"
     CDS             complement(52273..53355)
                     /gene="mraY"
                     /locus_tag="OO470_000349"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6249727.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53349..54707)
                     /gene="murF"
                     /locus_tag="OO470_000350"
     CDS             complement(53349..54707)
                     /gene="murF"
                     /locus_tag="OO470_000350"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6249728.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54704..56191)
                     /gene="murE"
                     /locus_tag="OO470_000351"
     CDS             complement(54704..56191)
                     /gene="murE"
                     /locus_tag="OO470_000351"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6249729.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56178..57944)
                     /gene="ftsI"
                     /locus_tag="OO470_000352"
     CDS             complement(56178..57944)
                     /gene="ftsI"
                     /locus_tag="OO470_000352"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6249730.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(57960..58325)
                     /gene="ftsL"
                     /locus_tag="OO470_000353"
     CDS             complement(57960..58325)
                     /gene="ftsL"
                     /locus_tag="OO470_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6249731.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58322..59263)
                     /gene="rsmH"
                     /locus_tag="OO470_000354"
     CDS             complement(58322..59263)
                     /gene="rsmH"
                     /locus_tag="OO470_000354"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6249732.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59265..59723)
                     /gene="mraZ"
                     /locus_tag="OO470_000355"
     CDS             complement(59265..59723)
                     /gene="mraZ"
                     /locus_tag="OO470_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6249733.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14280 a        16624 c        14729 g        14187 t
ORIGIN      
        1 gacccgagag gcaaagacga tacaacagga ggagtttacc cggatcaaga tacaggtata
       61 acataccctc tccatttcca ggccacgcta caacaggatg gaactatacc aatagaagct
      121 ggtgagttta aagccaccag tactttccag gtaacctacc cttaataagt ccaccgcacc
      181 gccatcatct ggcggtgcgg taattgataa atcattgccc ccaccaatgt gactgataaa
      241 atgccaggca acacgacatc atttatcagg atacgttatg aaaccgacca ccattgcttc
      301 acttcagaaa tataagcagg ataaaaagcg tttcgcgacc atcaccgctt acgactacag
      361 cttcgcgaaa ctatttgctg atgaagggct taacgtcatg ctggtaggcg actcgctggg
      421 catgaccgtt cagggccacg attccactct gccagtaacc gttgctgata tcgcctacca
      481 cactgccgcc gtacgtcgcg gcgcaccaaa ctgcctgcta ttggctgacc tgccgtttat
      541 ggcgtatgcc acgccggaac aagcctttga aaacgccgca acggttatgc gtgccggtgc
      601 caatatggtc aaaattgagg gcggtgagtg gctggtcgaa accgtaaaaa tgctgaccga
      661 acgtgccgtt cctgtatgtg gtcacttagg tttaacccca cagtcagtga atattttcgg
      721 tggctacaaa gtacaggggc gcggcaatga agcgagcgat cgactactca gcgatgcatt
      781 agccttagaa gccgcggggg cacagctgct ggtgctggaa tgcgtgccgg ttgagctggc
      841 aaaacgtatt accgaagcac tggcgatccc ggttattggt attggtgcag gcaacgtcac
      901 tgacgggcaa atcctcgtga tgcacgacgc ctttggtatt accggcggtc acattcctaa
      961 attcgctaaa aatttcctcg ccgaaacggg cgacatccgc gcggctgtgc ggcagtatat
     1021 ggctgaagtg gagtccggcg tttatccggg cgaagaacac agtttccatt aaggagtcac
     1081 gttgtgttaa ttatcgaaac cctgccgctg ctgcgtcagc aaattcgccg cctgcgtatg
     1141 gaaggcaagc gcgtggcgct ggtgcctact atgggtaatc tgcacaatgg ccatatgaag
     1201 ctggtcgacg aagccaaagc ccgcgccgat gtggtcgtcg tcagtatttt cgttaacccg
     1261 atgcagttcg accgcccgga agatctggca cgttatccac ggaccttgca ggaggactgc
     1321 gagaagctaa acaaacgtaa agtggattta gttttcgccc cttcggtaaa agagatctac
     1381 ccgaacggta ctgaaaccca cacttacgtt gacgttcctg gcctttcgac catgctggaa
     1441 ggtgccagcc gtccgggaca ttttcgcggc gtttcgacta ttgtcagcaa gctgttcaac
     1501 ctggtccagc cggacatcgc ctgcttcggt gaaaaagatt ttcagcaact ggcgttgatc
     1561 cgcaaaatgg ttgccgatat gggcttcgat attgagattg tcggtgtgcc aattatgcgc
     1621 gccaaagacg gtctggcgct aagttcccgt aacggttatc tgacggcgga acaacgcaaa
     1681 attgcgcctg gtctgtacaa agttttaagt tcgattgctg acaaattgca ggctggggaa
     1741 cgggatctcg atgaaattat taccattgcg gggcaagaac tgaatgaaaa aggcttccgc
     1801 gccgatgata ttcagattcg cgatgccgac acattgctgg aagtttctga aaccagcaaa
     1861 cgggcagtaa ttctggtagc cgcctggctt ggcgatgctc gcctgatcga caacaaaatg
     1921 gtcgagctgg cgtaatactt aactggcgct acggctgatg gcgccagtta ttaatttacc
     1981 ccacgtcatc tgtcataaca caggtaactt aaataaggtt aattacctta tcaatctcag
     2041 caagaggtaa atttgccatc tctgcaacgt cttcccgaga cattccttta cttagaagac
     2101 gcagggcgaa ttcttgcctt tcctcctgtc ttccctgctg aatccccttc tcaatccctt
     2161 tctcttcaaa ccactgcgcc agcgtcatca tagacttccc tcccgtttcc ctgtctctca
     2221 gcacaccata aaacaaatcc gcttgttcag tatgaccgcg ttgcagcata tagttttgca
     2281 tggcaactaa ctgacttccg ctagtgtacc cttcgtcaat cagcgtgacc agttgctcca
     2341 gcaataacat taagtcgcgc tggcgaatat gtttttgcag tagttcgaga atcgcaatcc
     2401 gccgatgttg catgatttcg tcatccggtg tgatggtgat atccaccagc gggaaaggac
     2461 tgttatagac gcgtcgcgcc agctccggcg agtaaaacat atcaaaccag cacattgaga
     2521 gcggataagg tgtggcctcg ccctgataaa acagtatcgg caccaccagc ggcagcttat
     2581 cgtggccagc ctccagatgc cggtgcatgg cagcaataga ataacgcatc atgcgaaagg
     2641 ccattttctt atccggcttg ctttggtgtt caatcacaac atgcagataa ccgggattgc
     2701 cctgcatttg catggaatag agcacgtccg tgctgtgacc tttcaggcac tcttcaatga
     2761 aactccccga ctctaaatga agcgtgttga gatcacaaag ttcgcgtaat tccactggca
     2821 aatgtatctc cagaaagtcg cgagccgtct ccgcatgcat taaaaattgt ttaaataccg
     2881 cgtcatgcgg tgtggtactc ggtgcatcca tcttccctgt cttccgtgtg gccttgcgtg
     2941 aaggcagtat ccagtcaagg cgtgctatcg tcagccacct ctttattgtt taccgagcag
     3001 cgttcataaa aatatcaatt cgttacaggc gttacatggc acgcttcggc gcggtacgca
     3061 aactgctgac gcaaatgcca tcagatgagt agacactcat caaaaatcgg gcaatactgc
     3121 gtgagaattt cctcaaagca ggcaccgcct gcttcgttaa cgacagggta gaaaggtaga
     3181 agttatgatt cgcacgatgc tgcagggcaa actccaccgc gtgaaagtga ctcatgcgga
     3241 cctgcactat gaaggttctt gcgccattga ccaggatttt cttgacgcag ccggtattct
     3301 cgaaaacgaa gccatcgata tctggaatgt caccaacggc aagcgtttct ccacttatgc
     3361 catcgcggca gaacgcggtt cgagaattat ttctgttaac ggtgcggcgg cccactgcgc
     3421 cagcgtcggc gatattgtca tcatcgccag cttcgttacc atgccagatg aagaagctcg
     3481 cacctggcga cccaatgtcg cctattttga aggcgacaat gaaatgaaac gtaccgcgaa
     3541 agcgattccg gtacaggttg cttgattgtt atccctgcgg ctggttactc accagccgcg
     3601 acatcttctc cagcgaatcc gttcttaaga tataaagtcg ttttagtaac aacggattat
     3661 cccccggttt tactttgcct ttcatggttg tcaccgccag gtgaaatccg gcatcgtttg
     3721 ctgccttcac ggctttgtca ttaaagccgc caaacggata cgaaagatac cagacatgtg
     3781 gattaaattg cgccagagcg cggcgtgaac gtgcaaaatc aaacagaata ttgtgctcac
     3841 tacggctcag taatatgggt cggcgataac catctacccg atgcaaaaaa tgggtatgtg
     3901 actggaaatc aaaaacatcg cgaatttcgt taagctcaga aacgctcata aattgcagcg
     3961 attttgggtt ccacttctgc gggtgacgtt tgatgcgtga ggtaacaata aacaccgtcg
     4021 ccttcatgcc atattgtttc aacacaggat acgcatagcg gctcaccgac ttgaggccat
     4081 catcaaaggt aatcaccacc gctcgcgcag ggagattgat cgtattcttc acgtaacctt
     4141 ccagctgagc catgctcagt gtcgcgtatc ccctgtcgcg cagccaggcc atttggttat
     4201 tgaaagcgcg taccgaggtg gtcgtcgaag tatggcgaaa acgggtgttt tcttcgtcgc
     4261 gcagaatatg gtgataagtt agcaccggca ggccattatc gggttgggca tccagcgcgc
     4321 tgatataggc cagtcgatcg ccaatacgga tctgatacca ggtttgattt aacctgtctt
     4381 tcagtttatg caaaatcggg tagcgcaaat tgtccgccag taccccaaat ggcgcactgc
     4441 ccacactcgg cgcgttgtag accggcgtat ctttccaggt aattaagttc tgattactca
     4501 gcggcttgtt gaggtcaccc aaaccgtctt caactttttg tcgcccctga actggctcga
     4561 gatgcccttt atcgataaac cctttaccaa agccaaaatt aaatgcgtaa taacttgcgg
     4621 cagtgggctc cacggcaatg atttgtccgg cccgaatatt ccccacggtc accatcttgt
     4681 cactaatttg cgcccagacc gcagcatttt cgatggtttg catataacgg gcaggtaacg
     4741 cggcactgac acttgcggta aacagcatca gcaggagaat aacagcttgt ttatacatga
     4801 aaaaataacc gagggtgaaa caacgctttt attgtagcaa aagcgccatc agaatcgcgg
     4861 gttaatactc atacaaatta tgtttgagga cgaaaggcgg atttttttgt tgttggtgcc
     4921 aaagactact cacctccggc ccacccagtt cgacaataca ctcacgaaac tctgaactgg
     4981 tcatggtttc acggcaagcc atcatctgtt caattaacgg taacgtgaca ccagaaatca
     5041 cttcgcaacg ggagtgtttg tggcttaata atgaagccac gcgatacggt ggcgctccgg
     5101 ctatatcggt taagaagatg acaccctcac cagagtccgc ttcatgcaga gcatcgcaca
     5161 tcatgcggct gagcatatta gagcttaatc cgcgccagaa attcacggcc cggcactgaa
     5221 gaagtgcccc atgttttttc tccagcgcat ccagtatctc ttgcgcccgg tcatcgtgac
     5281 aggtaattac ccaacctaac atcgccttcc tccttagccg aaaatcagcc taccctgacc
     5341 attgttgata aagggtgata gccgtcaaaa ctcctctccc ccgaatccag aggagaggaa
     5401 atagccttag ctacgcaaac cacgtccacg ttggatcagc gaccaacaga tcaaataaaa
     5461 cgccacaata aagaccacca gtacgccaaa ggtagtgacc agcggcacat catttatacc
     5521 gaggaagccg tagcggaaac cactgatcat ataaacgatt gggttcaggt gcgacagccc
     5581 ttgccagaac ggcggcaaca aagtcagtga gtaaaagacc ccgcccaaat acgtgagtgg
     5641 cgttaacaca aaggttggca ccaggctgat gtcatcgaac gttttggcaa acacgccgtt
     5701 cagcaaaccc gcaagggaga atagcaccgc cgtgagcacc aaggttaagg caacgaatac
     5761 ccacgaatgc acctgaaacg gcacaaaaaa cagtgaaatt gccgtcacca gaatgccaac
     5821 aaacagacca cgcgccacgc caccgccgac atatccggca ataatgacgt gagtcggaac
     5881 cggcgctacc agcagctctt caatattacg ctggaacttg gcaccaaaaa atgatgacgc
     5941 gacgttagcg taggcgttgg tgatcaccga catcatgatc agccccggta cgatgaactg
     6001 catatagctg aagccatgca tatcgccaat acgcgaacct atcaggttac cgaagataat
     6061 aaagtaaagg gtcatggtga tgactggcgg caccagcgtc tgcacccaga tacgcataaa
     6121 gcgatggatc tctttcgccc agatgctttt tagcgccacc cagtaaagat gcatcatgcg
     6181 cgatctcctt gtttttcatt aaccagtgaa acaaacagct cttccagacg gttagctttg
     6241 ttacgcatac ttaatacctg aatgccctgc tcacttaact gcgtaaatac gctgttgatc
     6301 ccctgctcac gtagcacttc aacttccagc gtcgcggtat cgaccagtcg atactgatag
     6361 ccatcgagct tcggtaacgg acttttcggt gcgagatcga gaataaaggt ttccgatttc
     6421 agcttcgcca gcagcgcctt catcgaggta ttttccacca gctcaccatg ttgaataatg
     6481 ccgatattgc ggcacagcat ttctgcttct tccaggtagt gtgtggtaag aatgatggtg
     6541 gtgcctttgt cgtttaaatc cttcaaaaag ccccacattg agcggcgaag ttcaatatcc
     6601 acgcctgcgg tcggttcgtc gagaatcagt agtttaggtt catgcattaa cgcacgggca
     6661 atcattaaac ggcgcttcat cccgccagat aacatacgcg cacgttcgtt gcgttttccc
     6721 cataaatcga gctgcttaag atatttttcg ctgcggatgt acgcctcttt gtgctccaca
     6781 ccgtagtacc ctgcctgatt caccacaatt tgctgaacgg tttcaaacgg gttgaagtta
     6841 aattcctgcg gcaccagtcc caactgacgt ttagcgttca cgacatcttt ctcgagatcg
     6901 taaccaaata cgctgacccg cccggaggtt ttatttacca gagagctgat aataccgata
     6961 gcggtcgatt tcccggcccc gttcggcccg agaagcgcat aaaaatcacc cgcttcgacc
     7021 tgcaaatcta ttccacgaag cgcctgaacg ccgcctggat agtttttttt aagctgttga
     7081 agttccagtg caatggtcat aaatttttac ttaccttacg ttcttacact ttatctgtgg
     7141 tttaaatcgt cccggagttg ccctatatta gccaaacgta attatttggt tacaggtcgt
     7201 taacctccat gaaagacata gatacactca tcagcaacaa tgcactatgg tcaaaaatgc
     7261 tggtggaaga ggatcccggg ttttttgaga aactggcaca agcgcaaaaa ccgcgctttc
     7321 tatggattgg atgttccgac agtcgcgttc ctgcagaacg tttaaccggt cttgagccgg
     7381 gcgaactctt tgttcaccgt aatgttgcta acctagtcat tcacaccgac ctgaactgcc
     7441 tttccgtggt tcagtatgca gtggatgtac tcgaagttga acacattatt atctgtggcc
     7501 actacggttg cggcggcgta caagccgccg ttgaaaaccc ggaactgggg cttatcaaca
     7561 actggctgct gcatatccgc gatatctggt tcaaacatag ctcattgctc ggcgaaatgc
     7621 cgcaagagcg ccgtctggat accttgtgtg aactgaacgt catggaacag gtgtataacc
     7681 tgggccactc caccattatg caatcagcgt ggaaacgcgg gcagaaagtt accattcacg
     7741 gctgggccta cggcattcac gacggcttgc tgcgtgatct ggatgttacc gccaccaacc
     7801 gcgaaaccct tgagcaacgt taccgtcacg ggatttccaa cctcaagctg aaacacgcca
     7861 accacaaata aaaatgccat gccggatgca acacatccgg caacttcaca cttactcgtc
     7921 cagcagaatc actttgccaa tatacggcag atgacggtaa cgctgtgcgt aatcaatgcc
     7981 gtaacccacc acaaactcat ccgggatcga gaaaccgata aattcaaccg ggacgttcac
     8041 ttcacgacgg gacggtttat ccagcagcgt acaaatcgcc aacgacttcg gttcgcgcag
     8101 gcttaagatc tcacgcactt tcgacagtgt attccccgag tcgatgatat cttcaacaat
     8161 cagcacgtcc ttgccacgga tatcttcatc cagatctttg aggattttca catcacgggt
     8221 ggtggacatg ccgctaccgt agctggaggc ggtcataaag tcgacttcat gagatacctg
     8281 aacttcacgg cacaggtccg ccataaacat aaatgagcca cgcagcagac ccaccagcac
     8341 catatcgctg ccgctgtctt tgtaacgctc agtaatctga cgacccagtt cggcgatacg
     8401 cgctttaatc tccgcttcgg ggatcattac ttctacagta tgtttcatat ctctaaccat
     8461 atgatttaaa aacaaatcac ttcgcacggg caggttttgg caccgcaaca aagcgcaaga
     8521 cgcgcagtat accagtaaaa ctattcattc gcggaatctg ttaataaagc tcgaattgtg
     8581 atgacggtca cacatgttaa aacctataat taattgctac taaaatatta atgaattgaa
     8641 atggtgtctc tttatggcaa ttaacaatac agcctcgcga cgattactcg tcacgctaac
     8701 agcccttttt gcagcgcttt gcgggctgta tctactcatt ggcggaggct ggctggtcgc
     8761 gattggcggc tcctggtact accctatcgc tggccttgtg atgctcggcg tcgcctggat
     8821 gctgtggcgc agtaaacgcg ccgcgctttg gctatacgca gccctgctgc tcggcaccat
     8881 gatttggggc gtctgggaag ttggtttcga cttctgggcg ctgactccgc gcagcgatat
     8941 tctggtcttc ttcggcatct ggctgatcct gccgtttgtc tggcgtcgcc tggtcattcc
     9001 tgccagcggc gcagttgccg cactggtggt cgcactgctg attagcggtg gtatcctgac
     9061 ctgggccgga tttaacgatc cgcaggagat caacggcacc ttaagcgccg atgccacacc
     9121 tgccgaagct atctcccccg ttgccgatca ggactggcct gcgtatggcc gcaatcagga
     9181 aggtcaacgc ttttcaccac tgaagcaaat tcacgccgat aacgtccaca agctgaaaga
     9241 agcctgggtg ttccgtactg gcgatgtgaa gcagccgaac gatccgggtg aaatcaccaa
     9301 tgaagtgacg ccgattaaag tgggcgatac cctttacctg tgtaccgctc accagcgcct
     9361 gtttgcgctt gatgccgcca gcggcaaaga gaaatggcat tacgatcctg agctgaaaac
     9421 caacgagtct ttccagcacg taacctgccg tggcgtctct tatcatgaag ccaaagcaga
     9481 aacggcttcg ccggaagtga tggcggattg cccgcgtcgt atcattcttc cagtgaatga
     9541 tggtcgcctg attgcgatta acgctgaaaa cggcaaactg tgcgaaacct tcgccaataa
     9601 aggcgtgctc aatctgcaaa gcaatatgcc agacaccaaa ccgggtctgt atgagccgac
     9661 ttctccgcca attatcaccg ataaaaccat cgtgatggcc ggttcagtca ccgataactt
     9721 ctcaacccgc gaaacgtctg gcgtgatccg tggttttgat gtcaacaccg gggagctgct
     9781 gtgggctttt gatccgggcg cgaaagatcc gaacgcaatc ccgtcagacg aacacacctt
     9841 tacctttaac tcaccaaact cgtgggcacc agcggcctat gacgcgaagc tggatctggt
     9901 ctatctgccg atgggcgtga ccacgccgga tatctggggc ggtaaccgca caccggaaca
     9961 ggaacgttat gccagctcga ttctggcgct gaatgccact accgggaaac tggcgtggag
    10021 ctaccagacc gttcaccacg acctgtggga catggatctt ccggcacagc cgacgctggc
    10081 ggacatcacc gttaatggtc agaaagtgcc gattatttac gctccggcga aaaccggtaa
    10141 catttttgtg ctcgatcgtc gtaatggcga actggtggtt ccggccccgg aaaaaccggt
    10201 tccccaaggt gctgccaaag gcgattacgt tacccctact caaccattct cggaactgag
    10261 cttccgtccg acgaaagatt tgagcggtgc ggatatgtgg ggagccacca tgtttgacca
    10321 actggtgtgc cgcgtgatgt tccaccagat gcgctatgaa ggcattttca cgccgccatc
    10381 tgaacagggt acgctggtct tcccgggtaa cctggggatg ttcgaatggg gcgggatttc
    10441 cgttgatcca aatcgtgaag tggcaattgc caacccaatg gcactgccgt ttgtttcgaa
    10501 actgctcccg cgtggtcctg gcaacccgat ggagcagccg aaagatgcca aaggcacggg
    10561 tacggaatcc ggcattcagc cacagtacgg tgtaccgtat ggtgtcacgc tcaacccgtt
    10621 cctctcacca tttggtctgc catgtaaaca gccagcatgg ggttatatct cggcgctgga
    10681 tctgaaaact aatgaagtgg tgtggaagaa acgtattggt acgccgcagg acagtatgcc
    10741 gttcccgatg ccggttccgg tgccgttcaa tatgggtatg ccgatgctgg gcgggccaat
    10801 ctccacggcg ggtaacgtgc tgtttatcgc cgctacggca gataactacc tgcgcgctta
    10861 caacatgagc aacggtgaaa aactgtggca gggtcgttta ccggcgggtg gtcaggcaac
    10921 accgatgacc tatgaagtga atggcaagca gtacgtggtg atctccgcag gcggtcacgg
    10981 ttcatttggt acgaagatgg gcgactatat tgtggcttat gccctgccgg atgatgtgaa
    11041 ataaccataa tgcccggaga gatccgggca tatttccgaa tacggtcttt ttataccgta
    11101 aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca
    11161 tgttctttcg gtgcgttgtg attaaacttc accagcactt cactgacatt gccttctacc
    11221 ttaacggtat ctttccagcc cgcgcgatga gccgctggcg gtttgccatt ttctgacaag
    11281 atacggaact gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag
    11341 ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac
    11401 gcctgaccgt tgattttatt ggcatggtgg aaatcgaact tcccgccgtg gttcatatga
    11461 ttcatattgc cgtgccccat atggcccatc atctggctgt gatccattcc ggccatcgcc
    11521 tgatcgccat atttctccat tagcatctgc atccccatca tatcgagcat cgggtccata
    11581 gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt
    11641 aatgtgtcag gcaaagcacc ggaggcacta atagcaatcg gctgaatccg cattaccgga
    11701 tgcggcttgt caaacggtgc aatcgccatc cccatctggc tgaccggcag cgtcaccagg
    11761 tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc
    11821 ggcagctcgc tcaccttcac cggttcaggt agcagaccac cgtcgctggc aatcacatac
    11881 agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa
    11941 cgcaggcgca gccaaccacg cggggcagcg tgttgtgggt agattgcacc gttggtcagc
    12001 agcgtatcgc caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc
    12061 ccgtcggcgc taaatttctt atcctgaacg atcaccggaa catcatcgat accccactgt
    12121 tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccagacc agccagcccc
    12181 atcgccacct gtcgcccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca
    12241 ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgtggg
    12301 ccgccgtcga cgtcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgcc
    12361 agttggttgt agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc
    12421 agcagattgc cgttatagcc ccaggtagtt gcagttttct cgccaaaggt ggactgacct
    12481 gcgccaatag ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt
    12541 aacgttgggc gttccgccgc aaagactgca cggctccaca gtggcaaggc tgaagccaca
    12601 cccagtgcga cggaatattt caagaaatca cgacgttgca tagttatttc cttattctta
    12661 agcaggcgaa acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc
    12721 gcagacacaa tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta
    12781 tcttctttaa gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca
    12841 gcctgatcgc cgtttgcgca aacagttacg cgctcagcga gtctgaagcc gaagatatgg
    12901 ccgatttaac ggcagttttt gtctttctga agaacgattg tggttaccag aacttatcta
    12961 acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta
    13021 attacgacac cttcgacatg aaagctctcg gtgaagacag ctaccgcgat ctcagcggca
    13081 ttggcattcc cgtcgctaaa aaatgcaaag ccctggcccg cgattcctta agcctgcttg
    13141 cctacgtcaa ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca
    13201 atgttagcta ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca
    13261 tggccgaaaa aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg
    13321 tagataacgt tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg
    13381 ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt
    13441 ttatctatca tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg
    13501 tgctgattat cggcggcggc gacggtgcca tgctgcgtga agtaacccga cataaaaacg
    13561 ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt gtcgttctgc cgtcagtatc
    13621 tgcccaacca taacgccggt agctacgacg atccgcgctt tcaactagtg atcgacgatg
    13681 gcgtcaattt cgttaatcaa accagccaga cctttgatgt cattatctcc gactgcaccg
    13741 atcctatcgg tcccggcgaa agcctgttca cctcagcatt ttatgaaggc tgcaaacgtt
    13801 gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag
    13861 aagccatcga cagccatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg
    13921 cggcgatccc gacctattac ggcggtatca tgacttttgc atgggcgaca gataacgacg
    13981 ccttacgcca tctctcaacc gaaattattc aggcgcgttt tctcgcttct ggcctgaaat
    14041 gccgttatta caatccggca atccatacgg cagcttttgc cttacctcag tatctgcaag
    14101 acgcactggc ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc
    14161 atggctttaa taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca
    14221 aaactgccga agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc
    14281 gtctgaccga aatcctgtca gaaacctgtt ccattatcgg ggccaatatt cttaacatcg
    14341 cccgccagga ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg
    14401 ttgacccgaa actcatcgac aaaacagaac accccggccc actgccagaa acggtggttg
    14461 ctcatctcga taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg
    14521 gtttatgtac cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga
    14581 aggcgctgaa ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg
    14641 tgcgcggttt tactcgcgac attaacggta tgaagcactt tatcgaccat gagattaatt
    14701 cgattcagaa ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaacg
    14761 tctatcagga aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact
    14821 acatgttcca caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg
    14881 cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa tatgccagct gtttaacggc
    14941 tctggcggag ctcccaggct ccgccagatt tatttacttc tgctgcacga aattgcggta
    15001 agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta
    15061 gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat
    15121 cacctcttca gcgtccatcc acagggtgta ttcatgccct gcccgttgcc aggaccgttc
    15181 gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca ggttttcttt
    15241 cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac
    15301 cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa
    15361 tgcgccgaaa tcgcatcgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag
    15421 cggaacggcg atagtttaga aataaaaaat gcccgggaat ctcattcacc gggcattgtg
    15481 tcgtttatgc gcagcgcgtg cgcttactta ttacaccgca gtctggaaaa tcaccccatc
    15541 ggctttttcg gtgtactgag aaagctggtt gaagttcaga taacgataag tatcaacggc
    15601 agttttatct acttgcgcaa cgtaggtctg gtactcttcc ggcgtcggca gtttgccaat
    15661 cagcgccgca acagccgcca gttctgcaga agccaggaag acattcgcgc cagtacccaa
    15721 acggttcggg aagttacggg tagaggtgga aaccaccgtc gcaccgtctg ctacacgcgc
    15781 ctggttaccc atacacaggg aacagcccgg gatctcgata cgagccccgc tcttaccgaa
    15841 gacgctgtag tagccttctt ccgtcagttg cgcggcgtcc atacgggttg gcggtgccac
    15901 ccacaggcgg gtcggcaact gacctttgtg cgcatccagc agtttaccag cggcacggaa
    15961 gtgaccgatg ttagtcatgc aagaaccgat aaatacttcg tcgatcttct cgccctgtac
    16021 cgcagacagc ggacgtgcgt catccggatc gttcggagca cacaggattg gctctttaat
    16081 ctccgccaga tcgatgtcga tcactgccgc gtattccgca tctgcatcgg cttccagcag
    16141 ctcaggattc gccagccatt tttccatgcc ctgaatacgg cgttccaggg tacgacgatc
    16201 gccgtaacct tccgcgatca tccacttcag caggacgatg ttggagctca ggtattcgat
    16261 gatcggttct ttgttcagct tgatagtaca accagcggca gaacgctcgg cggacgcatc
    16321 ggttagctca aaggcctgct caactttcag atccggcaga ccttcaattt ccagaatgcg
    16381 accagagaag atgtttttct tgcctttctt ctcaacggtc agcagacctt gtttgatcgc
    16441 atacagcgga atggcgtgta ccagatcgcg cagggtgatg cccggctgca ttttgccttt
    16501 gaagcgcacc agaacggatt ccggcatatc cagcggcatt acgccagttg cggcagcaaa
    16561 cgccaccaga ccagaacccg ccgggaaaga gataccgatc gggaaacggg tatgggagtc
    16621 accaccggta ccgacggtat ccggcagcag catacggttc agccaggagt gaatgacgcc
    16681 gtcacccgga cgcagcgaca caccgccacg gttcataatg aagtccggca gcgtgtggtg
    16741 cgtgttcacg tcaactggct tcggatacgc cgcggtgtgg cagaaagact gcatcaccag
    16801 gtcagccgag aagcccaggc acgccaggtc tttcagttca tcacgggtca tcgggccggt
    16861 ggtgtcctga gagcctacag aagtcatttt cggttcgcag tacgcgcccg gacgaatgcc
    16921 tttcacgcca caggcacggc ctaccatttt ctgcgccagc gagaagccgc gatcgctctc
    16981 agcgacatct ttcgcctgac ggaacacatc actgtgcggc agaccaagtg cttcacgcgc
    17041 tttggtggtc aggccacgcc cgataatcag cggaatacgg ccgccagcac gcacttcatc
    17101 aatcagcacg tcggttttca gttcgaaggt cgccagcagt tcgcctgttt cgtggttacg
    17161 cacttcacct ttgtacgggt aaacgtcaat cacgtcgccc atgttcaggt tagagacgtt
    17221 gacttcgatt ggcagtgcac ccgcgtcttc catcgtgtta aagaagatgg gtgcaatttt
    17281 accgccgagg cacaaaccac cgccgcgttt gttcggcaca tgtggaatat catcgcccat
    17341 aaaccacaga acggagttcg tggcggattt acgcgatgaa cccgtaccca caacgtcacc
    17401 gacgtacgcc agcgggaaac ctttctgttg cagagcttca atctgtttga tcgggccaac
    17461 aacacctggc tggtctggct caataccttc acgggcgttt ttcagcatcg ccagcgcgtg
    17521 cagtgggata tccgggcgtg accacgcatc cggtgccgga gaaaggtcat cggtgttggt
    17581 ttcgccagtg actttaaaga cggtaacggt cagtttttca gccagcgccg ggcgattcag
    17641 gaaccattcg gcatccgccc aggactgcat tacctgcttc gcatattcgt tgcctgcttt
    17701 cgctttctct tctacgtcat agaagttatc gaacatcagc agcgtgtgag aaagtgcttt
    17761 ggcagcaata ggtgccagtt tggcatcatc cagcgcgtcg atcagcggat gaatgttgta
    17821 accaccctgc atggtgccca gcagttcgat agctttttcc ggagtcagca gaggggattt
    17881 agcttcgcct ttagcgatag cagccaggaa gcctgctttt acataggcgg cttcatcgac
    17941 gcctggggga acacggttgg ttaacagatc taacaggaat tcttcttcgc ccgcgggcgg
    18001 gtttttcagc agctctacaa gcgcggccat ttggtttgca tccaggggtt tgggcgcaat
    18061 cccctcagcg gcacgctcag ctacgtgctt acggtattct tctagcacga cggttctcct
    18121 cgctctcatt gtcatagtgc ggcaggcgat tctcttcacg ctcctgtgag acagcagttt
    18181 gtagggtaaa tgcccagtac cgcaacgggc agaatagcag gattttgacg acgtgttaat
    18241 ctgtttacaa aaaagcaaca taaaaaagtg gctgaatcgt taaggatggt ctagagattg
    18301 tttccctccg taaaaacttc gttaattcgc atggtgataa tcacctttct caacgaatcc
    18361 caagattagt caaaatttaa actaccgcct ctttatactc ggattcacag cacctgcggg
    18421 tggcagttcg ccgaccattg cgatttcctt gagatccgaa ttatgaaaat gactttgccg
    18481 tttaaacccc atgtgctggc gctaatttgc agtgccgggc tttgtgctgc ctctgccggg
    18541 ctatatataa aaagccgcac agtggaagcg cctgtagaac cgcaatcgac acaactggct
    18601 gtgtctgacg ctgccgctgt tacgcttcct gcaacggttt ccgcacctcc cgtaataccc
    18661 gcagtcgtca aatccgcatt cagcactgca caaatagatc aatgggtcgc acccgtcgcg
    18721 ctgtatcccg acgccctact ttcgcaggtg ctgatggcat caacctatcc gacaaacgtt
    18781 gctcaagcag tgcaatggtc gcacgataat ccacttaaac aaggcgatgc tgctattcag
    18841 gcggtatctg accagccgtg ggacgccagc gttaaatcac tggtggcctt tccacaattg
    18901 atggcattga tgggcgaaaa cccgcaatgg gtgcaaaacc tgggcgatgc ttttctggcg
    18961 cagccgcagg acgtgatgga ctcggtacag cgattgcggc aactggcgca acaaaccggc
    19021 tcgctgaagt catcaaccga acagaaagtt attaccacaa cgaagaaagc tgtaccggta
    19081 aaacagacag tcacggcacc cgtcatacca tccaataccg ttttaactgc caaccccgtc
    19141 attacagagc cggcaacaac cgtcatttcc attgagcccg ccaatcctga tgtggtctat
    19201 attcccaact acaacccaac cgtggtttac gggaactggg ccaatactgc gtatccgccg
    19261 gtttatctgc caccaccagc cggagaaccg tttgttgaca gctttgtgcg cggattcggc
    19321 tatagcatgg gcgttgctac cacgtacgca ctattcagca gcatcgactg agatgacgac
    19381 gatcatgacc atcatcatca tgacgatgat gattatcatc accacgatgg cggtcatcgt
    19441 gacagtaatg gctggcaaca caacggcgac aacatcaata tcgacgtcaa caatttcaac
    19501 cgtatcaccg gtgagcatct tactgataag aatatggcat ggcggcacaa tccaaactac
    19561 cgtaatggtg tgccctatca tgatcaggat atggcaaagc ggtttcatca aaccgatgtc
    19621 aacggcggaa tgagtgccac gcagctacct gctccaacac gcgacagcca gcgtcaggcg
    19681 gcagcaagtc agtttcagca acgaacacac gccgcccccg tcattacacg agatacccaa
    19741 cgtcaggcag cggcacagcg gtttaatgaa gctgaacact atgggagcta tgacgacttc
    19801 cgcgacttca gccgtcgcca accactgacc cagcagcaaa aggacgccgc tcgtcagcgt
    19861 tatcagtcgg cctctcctga gcagcgccag gcagtccgcg agaaaatgca gactaacccg
    19921 cagatccagc agcgaaggga ggcggcgcgc cagcgtattc agtccgcctc gcctgagcag
    19981 cgccaggcgg ttcgcgagaa aatgcagact aacccgcaga accagcagcg aagagaggca
    20041 gcgcgtgagc gtattcagtc tgcaactccc gaacagcgcc aggtgtttaa ggaaaaagta
    20101 cagcagcgcc cactgaacca acagcaacgt gataacgccc gccagcgtat acaatcggca
    20161 tcacctgaac aacgtcaggt ttttcgggag aaagttcagg agagccgccc acaacgtcta
    20221 aacgacagta accatactgt caggctgaat aacgagcaac ggtcagcagt acgcgaacgt
    20281 ctctctgaac gtggagcaag aagactggaa aggtaaattg cagacgtaaa aaaagcggcg
    20341 tggttagccg cttttttaat tgccggatgt tccggcaaac gaaaaattac ttcttcttcg
    20401 ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac ttctgccgcc aggcccacag
    20461 actcgtgcag agtcgggtgc gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca
    20521 tttcgattgc caggccgatt tcacccagca gctcgccgcc gttggtacca acaatcgcac
    20581 caccgatcac acggtgagat tctttgtcga aaatcagctt ggtcataccg tctgcgcagt
    20641 cagaagcgat agcacgacca gaagcagccc acgggaaggt ggcggtttca tagctgatgc
    20701 ctttctcttt cgcttctttc tcagtcagac ctacccatgc aacttctggt tcggtatagg
    20761 cgatggacgg gataactttc ggatcgaagt agtgtttctt accggcgata acttcagcgg
    20821 caacgtgacc ttcgtgaaca cctttgtgtg ccagcatcgg ctgaccgacg atatcgccga
    20881 tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc aacgcggatg aaaccacggt
    20941 cgtcaacttc cacgccagct ttgcctgcgt cgaggttttt accgttcggc acacgaccaa
    21001 tcgctaccag cacggcgtcg taacgctgcg gttcagctgg tgcttttttg ccttccatcg
    21061 tcacataaat accgtcttct ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt
    21121 tgaatttctt gctgatacgc ttggtgaaga ctttaacgat gtctttgtca gctgccggga
    21181 taacctggtc gaacatttca accacgtcaa tctgtgaacc cagcgcgtgg taaacggtgc
    21241 ccatttccag accgatgata ccgccaccca ttaccagcag gcgttctggt acttctttca
    21301 gttccagcgc gtcagtggag tcccagatac gcggatcttc atgcggaata aacggcagtt
    21361 ggatcgggcg agaacccgct gcaatgatcg cattgtcgaa gttgatcacg gttttgccgt
    21421 tctcaccttc aacttccagg gtgttagccc cggtgaattt acccagaccg ttgaccactt
    21481 tgactttgcg gcctttcgcc ataccagcca gaccaccggt cagctgattg atcactttct
    21541 ctttccaggt acgaatcttg tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt
    21601 cagccagcgc tttggcttct tcgataactt ttgctacgtg cagcagtgct ttagaaggga
    21661 tacagccgac gttcaggcaa acaccgccaa gggtgttgta acgttctacg attacggttt
    21721 ccagacctaa atcagcgcaa cggaaggcag cggagtaacc tgcggggcct gccccaagta
    21781 ccacgacctg agttttgatt tcagtactca tcatgacctc tatatattta tctccggcgg
    21841 tcatacccgt cgtctttcag gccgcagcgg cgttagcttc acgccctcac cccagtcact
    21901 tacttatgta agctcctggg gattctggcg cttgctacct tgctgcaccc agaaatccat
    21961 agggtatgtg aagtaatacc ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca
    22021 aaattgttaa caatttttaa acaacaaacg gcaaccgatt tgtctattcg ctaataacct
    22081 caatacattc atgagattac cagaaaaaag ccggccgttg ggccggctct tttacttaca
    22141 tcaccagacg gcgaatgtca gacagcgtgt tgttaatgat ggtaatgaaa cgggcaccat
    22201 cagcaccgtc gatcacgcgg tggtcgaagg agagagaaat cggcagcatc agacgcggca
    22261 cgaactcttt accattccac accggctcca tcgcggactt ggaaacgccg aggatagcca
    22321 cttccggcgc gttcacaatc ggcgcgaagt gggtagtacc caggccgccg atgctggaga
    22381 tggtgaagca accgccctgc atttcgcccg cagtcagctt accgtcacgc gctttcttag
    22441 aaatagtcat cagctcgcga gacagctcga tgatgccttt cttgttgacg tctttgaata
    22501 ccggaacaac cagaccgttc ggggtatcca ccgccacacc gatgttgatg tatttcttca
    22561 gggtcagacg ctgaccgtct tccgacagcg aactattgaa gcgaggcatc tgctcaagag
    22621 ctgcagcaac ggctttcatg atgaagacaa ccggggtgat cttcacatcc agcttacgtt
    22681 tcgccgcttc ttcgttctgc tgtttacgga acgcttccag ctcggtgata tcggttttgt
    22741 cgaagtgggt aacatgcggg atcatcaccc agttacggct caggttagca ccagaaattt
    22801 tctggatacg gcccagttcc acttcttcga tttcgccaaa cttgctgaag tccaccttcg
    22861 gccacggcag catgccaggg ataccaccgc cagtcgccgc cggagctgct tctgcacgtt
    22921 tgatagcttc tttcacgtaa gcctgaacgt cttcgcgcag gatacgacct ttacggccag
    22981 tgcccttcac tttcgccagg ttaacgccaa actcgcgtgc cagacggcgg atcagcgggg
    23041 tcgcgtgaac gtaagcgtcg ttttcagcaa attcagattt gccttccgct ttcgcagccg
    23101 gagcttcagc ttttgctgcc ggagccggcg ctgccgcttc ctgtttcgca ggagctgccg
    23161 caggcgctgc gccttcaact tcgaagatca taatcagcga accagttttc actttatcgc
    23221 caacgttgac tttcagttcc ttcacgacgc ctgcaaacgg agccggaact tccatagaag
    23281 ctttatcgcc ttctacggta atcagtgact gttcagcggc aactttgtcg cccactttca
    23341 ccatcacttc ggtcacttca acttcgtcac cgccgatatc cggaacgtta acttctttca
    23401 cgccagctgc tggtgcaggg gtcgctgccg gagtcgcttc ctgtttagcc gccggagctg
    23461 ccgcgcctgc ttcacccgcg acttcgaaga ccataatcag cgagccggta gacactttgt
    23521 cacccacgtt cactttgatc tctttcacgg tacctgcaaa cggagccgga acttccatag
    23581 aagccttgtc gccttctacg gtgatcagcg actgttcagc ttcaacttta tcgccaactt
    23641 tcaccaggat ttcggtcact tcaacttcgt cgctgccgat atccggaacg ttaacgtctt
    23701 ttgccgccgc agccgcgggt gctgctgccg gagctgcttc tttcttctct tctgcctgag
    23761 caggtgcagc gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct
    23821 gggttttatc gccaacagag actttgatct ctttaacgat acccgcctgc ggagatggaa
    23881 cttccataga ggctttgtcg ccttctacgg tgatcagcga ctgttcggct tcaactttgt
    23941 cgcccacttt gaccaggatc tcggtgattt caacttcatc agccccgatg tccggtactt
    24001 tgatttcgat agccattatt cttttacctc ttacgccaga cgcgggttaa ctttatctgc
    24061 atcgatgttg aatttggcga ttgcgtcagc aaccactttc ttatcgattt cgccacgttt
    24121 agccagttcg cccagcgccg caaccacgac ataagaagca tcaacttcga agtggtgacg
    24181 caggttctca cggctgtcgg aacgaccgaa gccatcagta cccagtacgc gatagtcgtc
    24241 agccggtacg taagtacgga cctgctcagc gaacagtttc atatagtcgg tagatgccac
    24301 tgccggcgcg tcgttcatca cctgagcgat atacggaacg cgcggagttt ccagcggatg
    24361 cagcatgttc cagcgttcac aatcctgacc atcacgcgcc agctcggtga aggaggtcac
    24421 gctataaacg tcagaaccta cgccgtaatc tttcgccagg atctctgccg cttcacggac
    24481 gtgacgcagg atggaaccgg agcccagcag ctgaacttta cctttgctac cttcaatggt
    24541 ttcgagtttg tagatacctt tacggatacc ttcctcagca ccttccggca ttgccggcat
    24601 gtggtagttt tcgttcagcg tagtgatgta gtagtaaacg ttctcttgtt tttcaccgta
    24661 catacgctcc agaccgtcat gcataatgac agcaacttcg taagcgtaag ccgggtcgta
    24721 agagatacag ttcgggatag tcagcgactg aatgtggctg tgaccatctt cgtgctgcag
    24781 accttcaccg ttcagggtgg tacgaccgga agtaccgccg atcaggaagc cacgcgcttg
    24841 ctggtctcca gccgcccagc acagatcgcc gatacgctgg aagccgaaca tcgagtaata
    24901 gatgtagaat gggatcatcg gcagattgtt cgtgctgtaa gaggtcgccg ctgccagcca
    24961 ggaacaccct gcgcccagct cgttgatccc ttcctgcaga atctgacctt tctcgtcttc
    25021 tttatagtaa gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta
    25081 aataccaatc tgacggaaca gaccttccat accgaaagta cgcgcttcgt cggcgatgat
    25141 cggtaccaga cgatctttga tcgacttgtt cttcagcatc acgttcagag cacgaacgaa
    25201 agcgatagtg gtagagagct ctttgctctg ctcttccagc agcgcgccga agtcttgcag
    25261 gctcggcagc tcaagcttct cggtgaagtt cggctgacgg cttggcagat aaccgtgcag
    25321 tttctgacgc tgagcgtgca gataggtatg ctcttcagaa ccttccggga aggtgatgta
    25381 cggcagtttt tcgatatctg catcagacac cggcacattg aaacggtcgc ggatgtgacg
    25441 cacgccgtcc atgttcattt tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc
    25501 gcccatgccg taacctttaa tggtatgagc aaggattact gtcgctttgc ctttggtttc
    25561 ctgcgctttc ttgaatgcag cgtagatttt cttcggatcg tgaccaccac ggttcagtgc
    25621 ccagatctgc tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat atttaccgaa
    25681 gaagtgttca cgaacgtacg caccatcttt cgatttgaag gtctggtagt cgccgtcaac
    25741 ggtttcgttc atcagctgga tcagtttacc gctggtatct ttacgcagca gttcatccca
    25801 acggctaccc cacatcactt tgatcacgtt ccagccagca ccttcgaaga tgccttccag
    25861 ttcgttgatg atcttgccgt taccggtgac cgggccgtca agacgctgca ggttacagtt
    25921 gataacgaaa accaggttat ccagtttttc acgggtagcg atggtgatcg cacctttgga
    25981 ttccggttcg tccatttcac cgtcgccgag gaacgcgtaa acggtctgtt tagaggtatc
    26041 tttcaggcca cggtgttcca gatatttcag gaatttagcc tggtaaatag caccaatcgg
    26101 acccagaccc atagatacgg tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg
    26161 ataggaagag aggccattgc cgtgaacttc ctgacggaag ttatccagct gctcctgagt
    26221 cagacgacct tccaggaaag cacgggcgta aacgcccggg gagatgtggc cctggaagta
    26281 aaccaggtcg ccgccatcct gctcgttgcg tgcacggaag aagtggttaa agcacacatc
    26341 ataaatggtt gcggaagact ggaaggacgc catgtggccg cccagttcga ggtctttttt
    26401 cgacgcacgc agcaccgtca tgatggcgtt ccagcggata gctgaacgaa tacggcgttc
    26461 cagttccaga ttacccggat actccggttg ttcttcaacg gggatggtgt tgatgtagtt
    26521 gctgatacct gtgcctgcgg ctacgtttac accgcctttg cgggcttcag caagcagttg
    26581 gtcgatcaga tactgagcac gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg
    26641 gagccagtcg cgagtttcga tcggatccac gtcatttggg aaacgttctg acatgggtta
    26701 ttccttatct atctaataac gttgagtttt ctggaacctg tcccattgaa ctctcgccag
    26761 aaagctcaat aagacaggtt ctacgtttag ttaccgcgct tttatatgcg cttgatttac
    26821 aacatcttct ggataatttt taccagaaaa atcactaatt ctttcgttgc tccagacgac
    26881 gcagagaacg ctcacggcgg ctctcttcac ggcttctgtc gagcaaaatt tcttcgataa
    26941 aggccagatg gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt
    27001 caaatatgcg ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc gaatagagca
    27061 attcgaagtt ctggcggaca ttctgggcca acatcggctc catacacctt agcagatgaa
    27121 gcagaaccac attgtgggcc gcttcggtga cggcaatctg atactggagt acggcgtttg
    27181 attccgcgtc cagatcgcca gactgctgcg ccagctctat ggcgtggtgg agttcacgga
    27241 tgcgttcctt gtcttcatcg gtactacgca gcgcggcgta ataagcggcg ataccttcca
    27301 gggcgtgtcg tgtttcgagc aagtcatact gtgattcagg atggtcggag agcagctcca
    27361 ccagcggatc gctgaagctt tgccataggc tgctctggac aaaagtgccg ccaccctggc
    27421 gacgaagcaa caagcccttc gcttcgagac gttgaatcgc ctcacgcaag gagggacggg
    27481 agacgtcgaa ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc gggcggagag
    27541 tgccttcgag gatcaaaaac tccagttgct gctcaatcac atcggagagt tttggttggc
    27601 ggattttgct gtaggccatg agttcctgtc ttaagccact tgccgaagtc aattggtctt
    27661 accaatttca tgtctgtgac gctaaagtaa caaagtattc accttatgtc catacaggtt
    27721 ttgattgaaa tcatgaaact gtgcacattt taacaacttg acatatataa cgtttcaaag
    27781 ttgtaactat gcacaaatgt agactttacg taggaaaggg attttttaac gattattaaa
    27841 ctataaaaat ccgaattgaa ccgattcact taccaatttt ttgattttta agtcaataaa
    27901 aacgaattta aattcactct gcatattgag aggggttgag gctgagcttt acaaacggtt
    27961 tctttttaag caactcatct tcaaccatgc ataaagcggg tgcattcgct gccgcatacc
    28021 attattcttg atctgacgga agtctttttg taacaattca aacttccttg atgtaaataa
    28081 atcaatacaa caaacggaat tgcaaactta cacacgcatc actgcgtaga tcaaaaaaac
    28141 aatcaccgca cgaggtttca tgatggaagg tcaacagcac ggcgagcagc taaagcgcgg
    28201 ccttaaaaac cgccatattc agcttatcgc gctgggtggc gcgataggga cagggctatt
    28261 cctaggtagc gcctccgtaa tacagtccgc agggccaggg attatcctgg gttacgccat
    28321 tgctggtttt atcgcctttc tgatcatgcg tcagctgggt gaaatggtgg tcgaagaacc
    28381 tgtcgcaggc tcctttagcc actttgctta taaatactgg ggcagttttg ccggtttcgc
    28441 ctctggctgg aactactggg tactgtacgt tttagttgcc atggcagagc tgactgccgt
    28501 gggtaaatac attcagttct ggtatccgga aatcccaacc tgggtttctg ccgccgtatt
    28561 ctttgtggtg attaacgcca tcaacctgac caacgttaaa gtgtttggcg agatggagtt
    28621 ctggtttgcc attatcaaag ttattgcggt agtagcgatg atcatcttcg gcggctggct
    28681 gctgttcagt ggcaacggcg gcccgcaggc gagcgttagc aacctgtggg atcaaggtgg
    28741 tttcctgccg cacggcttca ccggactggt gatgatgatg gcgattatca tgttctcgtt
    28801 cggtggtctg gaactggtgg ggatcaccgc agcagaagct gataacccgg agcaaagtat
    28861 cccgaaagca actaaccagg ttatctaccg catcctgatt ttctatattg gttcgttagc
    28921 cgttctgctc tcactgatgc cgtggacccg cgttaccgcc gataccagtc cgtttgtgct
    28981 gatcttccac gagttaggcg atacctttgt ggcgaatgcg ctgaacatcg tggtactgac
    29041 tgcggcgctc tccgtgtaca acagctgcgt atattgcaac agccgtatgc tgtttggtct
    29101 ggcacaacag gggaacgcgc caaaagcgct ggcgtctgtc gataaacgcg gcgtaccggt
    29161 taacaccatt ctggtgtctg cgctggttac agcattgtgc gtattgatta actatcttgc
    29221 tccggaatcc gcatttggcc tgttaatggc actggtggta tccgcactgg tgatcaactg
    29281 gacgatgatc agtctggcgc atatgaagtt ccgtcgcgcc aagcaggaac aaggcgtggt
    29341 aactcgcttc cctgctctgc tttatccgct gggtaactgg atctgcctgc tgtttatggc
    29401 ggcggtactg gtgattatgc tgatggcccc aggaatggcg atttcggtat acctgatccc
    29461 ggtatggctg atcgtgttag gtatcggcta tctgtttaaa gagaaaaccg ccaaagccgt
    29521 aaaagcgcat taatctctct acgccctcac ccgtacaggg tgagggcaat aatctctaca
    29581 ccaacgcccc gtaaatcgtc agtagcgcaa tcaccaccac gaccacgaac gaggttttct
    29641 tcgccattga aaccgctgcc ttcggcgtct ccaccttatc gacatgcggt tcacgcgcca
    29701 gagagaactg cgccagacgc gttaatacct gatattgcga agtatggaaa tcacccagcg
    29761 aagcaaacca ggccggtaac gctttctcac catgaccgat caaggcatat accacacccg
    29821 caagacgaac cggcacccaa tccagtacat gaagcactgc atcaatgccg gactgtaaac
    29881 gatgatgcgg cgtctggtat cgcgccagcc agtattgcca tgcacgcaag aacgcatacc
    29941 ccatcagcgt aacgggtccc caggttcccc ccacaatcag ccagaacagc ggtgcaagat
    30001 aaaaacgaaa gttaatccac agcaatgcat tttgcagctc acgcaaatac tcacgttcgt
    30061 cgcagcctgc cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg gcacgggcat
    30121 ggctatcatt acgtgaagca gctgtcagat aagcatgata atgaagacga actttacctg
    30181 cgccaataca cagcaaacca atcagcagcc acaccagcag cgtgggaacg ttgaacaata
    30241 ctccctgcaa tgcgcgtaac agtaaaaaag tcacacccat cgcaataatg gtcatgccta
    30301 acgtgcgccc gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga cgatgatcaa
    30361 gctgccagtg ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt aaggttgtaa
    30421 atagcgtcat gttgtctcct tgctgaccag cgcacgaaac cgtgcccaat caaatgcagg
    30481 accgggatcg gttttccgat ccggcgcaat atcacaatgg cccgtcatgt tattagcgat
    30541 atcgggatag cgatctatca gtgcccgcgt aaccgccgca agctgttgat actgcgcatc
    30601 ggtatacgcc agagtatcgg tgccttcaag ctcaatccca atagaaaaat cattgcagcg
    30661 ttcacgcccc tgatactgag agactcccgc atgccaggca cgtttatcga aaggaacata
    30721 ctggactatt tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca aatgggcgat
    30781 ctcagcaaag aaaggatgtg cctgcggatc aatagttcca gtgaataatg cgtcgatcca
    30841 cggaccgcca aactcgcctg gcggcaggct aatattgtgc accaccagca gggtgggtgt
    30901 ttcgtcatcc aggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc caaccagcca
    30961 cccctgttct aacaacatgc aggatctcct tatatgtggt gctaataccc ggttcagagt
    31021 agcatgtttc taccttatga ttcgttagct atctggagtt ttaacatgcc gcctcgccgc
    31081 tacaaccctg acacccgacg tgacgagctg ctggaacgca ttaatctcga tatccccggc
    31141 gcggtggccc aggcgctgcg ggaagattta ggcggaacag tcgatgccaa caatgacatt
    31201 acggcaaaac ttttaccgga aaattctcgc tctcatgcca cggtgatcac ccgcgagaat
    31261 ggcgtatttt gtggcaaacg ctgggttgaa gaggtgttta ttcaactggc aggcgacgat
    31321 gtcaccataa tctggcatgt ggatgacggc gatgtcatca acgccaatca acccttgttc
    31381 gaacttgaag gcccatcccg cgtgctgtta acgggcgaac gcactgcgct taattttgtg
    31441 caaacccttt caggagttgc cagtaaggta cgccactatg tcgaattact ggaaggcacc
    31501 aacacgcagt tgttggatac gcgcaaaacc ttacctggcc tgcgttcagc tctgaaatac
    31561 gcggtacttt gcggcggcgg agcgaatcac cgtctggggc tttctgatgc cttcctgatc
    31621 aaagaaaacc atattattgc ctccggctca gtgcgccagg cggtcgaaaa agcgtcctgg
    31681 ctgcatccgg atgcgccagt agaagtcgaa gtagagaatc tggaagaact tgatgaggcc
    31741 ctgaaagccg gagccgatat catcatgctg gataacttcg aaacagaaca gatgcgcgaa
    31801 gccgtcaaac gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt cactgacaaa
    31861 acactgcgcg aatttgccga aacgggcgtg gactttatct ccgtcggtgc gctaactaaa
    31921 cacgtacaag cactcgacct ttcaatgcgt tttcgctaat tgctgacaga tttatccgct
    31981 ccatcgcggg cggataatct ccccttcccc aactttcttc gtaacgcctc gcaaatttct
    32041 tgtctttcgc tgcaacgaaa taaaaaacct cggaatctgc tttcccgttt cgttttttgc
    32101 ccctcgcctg tgctgatggc gtttgccaaa gtagcaccaa ccaaatcaag gagcgaaaca
    32161 gatggacaag caacgcggtt ttacacttat cgaactgatg gtggttattg gcatcattgc
    32221 cattttaagc tccattggta ttcccgctta tcaaaactac ctgcgcaaag ccgcactcac
    32281 cgacatgcta caaacctttg tgccttaccg taccgccgta gagttgtgcg cgctggaaca
    32341 tggtggatta gatacctgcg acggtggcag caatggcatt ccctcgccta ccaccacccg
    32401 ctatgtttca gccatgagtg tggcaaaggg cgtggtgtcg ctgaccgggc aagaaagtct
    32461 caatgggcta agcgtcgtca tgacgccagg ttgggataac gcaaacggcg tcaccggctg
    32521 ggcgcgcaac tgcaatattc aaagtgacag cgcattgcag caagcctgcg aagatgtctt
    32581 ccgctttgat gacgccaact aaggagcggc aatgaatatt ccacagctca cggccctgtg
    32641 cctgcgttat cagggagtct tgctggatgc cagcgaagaa gtggttcatg ttgcggtggt
    32701 cgatgccccc tcacatgagt tgctggacgc attgcatttc gctaccacca aacgtattga
    32761 gatcacctgc tggacgcgcc aacaaatgga aggtcacgcc agtcgcacac aacagacatt
    32821 acccgtagct gttcaggaga agcatcagcc caaagcagaa ttgctgactc gaacgttaca
    32881 atctgcgctg gaacaacgcg cgtctgatat tcatatcgaa ccagcggaca atgcctaccg
    32941 catccgcttg cgtatcgacg gcgtattgca tcctttaccg gatgtttcac cggatgccgg
    33001 agtcgcatta acagccagat taaaagtgct gggaaacctg gatattgcgg aacatcgcct
    33061 gccgcaggac gggcaattca ctgtcgaact ggcaggaaac accgtctcat ttcgtattgc
    33121 gaccttacca tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc aggtgagcca
    33181 ggcactggat gttaacacgc tgggaatgca gccgttacaa ctggcggact ttgctcatgc
    33241 cttgcaacaa ccacagggac tggtgctggt aactggccct acaggcagtg gcaaaacggt
    33301 cacctggtgt tgtccacgct acacaccaat tccaccactg aaacgctggt acgtttacag
    33361 caaatggggg tcgcccgctg gatgctatca tcggcgctta cgctggtaat agcccagcgt
    33421 ctggtacgta aactttgccc acattgtcgc cgacagcaag gggagcccat ccatattcca
    33481 gacactgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt acattgctac
    33541 cacggttttt atggtcgtac cgccttgttt gaagtcctgc ccattacacc agccatacgc
    33601 cagcttattt ccgctaatac tgaagttgaa tcgctggaaa cgcacgcccg acaggcgggt
    33661 atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac cacctttgaa
    33721 gagttaatcc gcgtactggg gatgccgcat ggcaagtaag caactctggc gctggcatgg
    33781 cataaccggc gacggcaatg cgcaagatgg gatgctatgg gcagagagcc gtactttact
    33841 gcttatggca ctacagcaac agatggttac cccactaagc ctgaagcgaa tcgccatcaa
    33901 ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg cgacgctact
    33961 taaagccggg ttaacgcttt ctgaagggct ggctctgctg gcggaacagc atcccagtaa
    34021 gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca ttgctttttc
    34081 caatgcctta ttaccctggt cagaggcatt tccgccgctc tatcaggcga tgatccgcac
    34141 gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc agcaaaaatc
    34201 ccagcgtcag ttgaccgaca aagtgaaatc agcgttgcgt tatcccatca tcattttagc
    34261 gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt ttgccgctat
    34321 ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga cgctggcaga
    34381 ctttagtggc gaatggggct ggctgctggt gttgttcggc tttctgctgg cgatagccaa
    34441 taagttgctg atgcgccgcc cgacctggct tatcgcgcgg cagaaattgc tgttacgcat
    34501 cccgattatg ggttcactga tgaggggaca aaaactcacg cagatcttta cgattctggc
    34561 gctgacacaa agtgcaggca ttactttttt acagggcgta gagagcgtca gagaaacaat
    34621 gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca gtaacggtca
    34681 tcccatctgg ctggcgctaa aaaatgccgg tgagtttagc ccgctctgtt tgcaattagt
    34741 gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg cccatcatca
    34801 tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac cggcgttgct
    34861 gatcataacg ggaggaatta tcggtacgct ggtggtggca atgtatctgc caattttcca
    34921 tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac gccagcgttg
    34981 ggagattaca ggttgttgaa gatgcggttt tcttgttcca gcacacgaat aaacgtggtg
    35041 cgcttggtca gctctttcag gcgtgaagcc ccaacgtatg tacaagctga acgcaggccg
    35101 cccaaaatat cacgcgcggt attttcaacc tggcctcgca gcggcagctt aacggtttta
    35161 ccttctgctg cgcggtattc cgcaacgccg ccaacgtgac gtttcatcgc agactcggag
    35221 ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg accgccgctc
    35281 tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc gccaaaggct
    35341 ttcgccacat cgcccggcgt ggtacagcca ccgtcgctga cgatcattcc gcccagaccg
    35401 tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac gcctgttttg
    35461 acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc agcaccggag
    35521 aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat ggttttggtc
    35581 ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata accattcgcc
    35641 acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt agttttttcg
    35701 aaatccgcat cagacgtgcc ggtagaaacc atcacatgtt tcagcacatc agcggaagaa
    35761 ttgttgataa acgcttgcca ctcttcgaca gaaaagtgtt tatgcacagc agtcagaata
    35821 tcaaaagaag ccagcgcaga ggccatagaa aatgtgccta cggtgtccat atttgcggcg
    35881 ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg acgttccagt
    35941 tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac gtctttaaaa
    36001 cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat ggctataagg
    36061 gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg caagactgcg
    36121 aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt gagcgaacgt
    36181 ttcatcaatt tcactttcgc gcgctcattt ttaacgtttt gataatcatg atgaattccc
    36241 gggaatataa attatggggt atatagttgc cttaacggga ggcattggca gtggtaagag
    36301 taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg atattattgc
    36361 gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc actttggcga
    36421 aaacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc ggatcttcgc
    36481 caacccggaa gagaaaaact ggcttaacga cctgctgcat ccgctgattc agcaagagac
    36541 gcaacaccag atccagcaag caacttcccc ctatgtactg tgggttgtgc cattgctggt
    36601 agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca gcccagaaac
    36661 gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gaacatgtcg aacaaatcct
    36721 tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca ttgataataa
    36781 cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact atttgcagct
    36841 tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct ttttgaacat
    36901 ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca gcaactcacc
    36961 gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa tgtcagtgaa
    37021 ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga acttaaccgg
    37081 cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag ccgtattgaa
    37141 gcattaattc agcagttaaa agcggcggtg agcgtattaa tttccgcgcc gcgtatcggg
    37201 caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag catcccaggc
    37261 ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc ccaggcgcag
    37321 cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca ggcacttacc
    37381 atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag cctgaatggt
    37441 ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc gctcgattca
    37501 cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt tatgccgctg
    37561 gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg ttgctaagga
    37621 gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac ggtggtgtgg
    37681 ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat cgacctcgga
    37741 gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga aagcgatgac
    37801 tggagcgaag agccaaagca gtgacatttg tctgatgcct cacgtaggcc tgataagacg
    37861 cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa cgccttatcc
    37921 gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc
    37981 attaagaccg accagcgaca tccactcacc gggttgccct tctttacccc acggcacccc
    38041 ttcccaactt tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatattc
    38101 cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acctcttcct gaagttcacg
    38161 caccaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa actccagttt
    38221 attcgccatg tgcgcatctg ctgcgcgacg tgttataaag atttcattgt tctcgttgcg
    38281 aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa aaaggcgcag
    38341 aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg gcactgcttg
    38401 tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ttcaccggtt
    38461 tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt
    38521 tgtgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc
    38581 atacgtacct gaactttgct cagcgtactg ataacttcat atttcaacga ctccagcatc
    38641 gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag
    38701 ccacgcaggt ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg
    38761 gagtcaagcg tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc
    38821 acttcttctt tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc
    38881 tcttcatgca gttctggttc tttatccagc cactcggcaa ttggtaaatc gaggtcgaaa
    38941 tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc
    39001 ggaatgtagg catcaatggt ggctttgaac acatcttcac gaatgctgtt gatggtttcg
    39061 ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg
    39121 ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact
    39181 ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc
    39241 ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc
    39301 gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga
    39361 ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg
    39421 ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt
    39481 tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc tccgagcaca
    39541 atatctgtac cacgacccgc catgttggtc gcgatagtca ccgcagccgg ataacctgcc
    39601 tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacatt gtgcttaata
    39661 ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat agtacccacc
    39721 agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt
    39781 tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc
    39841 ggaacaacga cggtatccag tttgtagatg gagctaaatt cgaaagcttc ggtatcagca
    39901 gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa
    39961 gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc
    40021 agaccatcgg accagcgtcg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata
    40081 acttcaccat ctttaacgat gtagtcgacg tcacgggtaa acagcgcatg agcgcgcagc
    40141 gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca
    40201 tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg
    40261 ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag
    40321 tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc
    40381 gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg
    40441 gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg
    40501 aacgccatgt tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg
    40561 tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt gataccaaca
    40621 gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag
    40681 tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta agcaggcagc
    40741 gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga
    40801 accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc
    40861 tcacgcacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga
    40921 cgcgcacgaa actctgcggt tttccctttc agttcttcat cggagagttt ttccatctcc
    40981 ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta
    41041 cgactaccga aaactttagt taacaatttg attagcataa taaaatctca aacgccccgc
    41101 gttacggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc gttgagggcc
    41161 agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt
    41221 aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcagcag
    41281 cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc
    41341 aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg tacgaatagc
    41401 atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgcgtgt tcgcttccag
    41461 caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt
    41521 tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcgac
    41581 catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag
    41641 tattccactc acgttattgc catcccgatc gaattcaaac gtatttagcg cacaaatctt
    41701 atcattaaag cctgaagtcc acagcacgaa tgaagataag ttgtgcgtaa aagtgcttaa
    41761 gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa aacgactggt
    41821 tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt
    41881 cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg
    41941 aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag tttgttattc
    42001 agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac
    42061 aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg
    42121 tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg
    42181 cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg
    42241 ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa
    42301 tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta
    42361 aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttt
    42421 ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg
    42481 ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc
    42541 aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg
    42601 accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc
    42661 ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac
    42721 gtcagggtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta
    42781 agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac
    42841 tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc
    42901 ttaatcagct tgcttacgta ggaatgctgg gatatccaga taatccggct ctttcgcagt
    42961 ttgcggcgca ttgtcattca ctactttagc aaccggcttc tgctcctggg tcagcggagc
    43021 catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag
    43081 agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta cgcgcagctc
    43141 gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa
    43201 tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgccgt
    43261 gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca gcggagaaga
    43321 gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat
    43381 cattgcgtag cccatttcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa
    43441 acccggacga gtaatcagtt cggcgatacc ttgcacagcg cctttcagta catcgttcgc
    43501 tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt
    43561 cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct gctcggcgaa
    43621 tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat
    43681 acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc
    43741 catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca atgcatcgcg
    43801 atcctcatca gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt
    43861 gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc
    43921 ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt gttcaacagc
    43981 attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat
    44041 tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg
    44101 gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc
    44161 aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc
    44221 atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc
    44281 ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc
    44341 tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg
    44401 tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt
    44461 gagcaactcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg
    44521 cggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc
    44581 cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc
    44641 gtaagcgata tcactggtca cgacattgcc agcataagga attacctttg tgtggcgcaa
    44701 tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga cgacgcagac
    44761 acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat
    44821 cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc
    44881 gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acacccgaaa gtcctaccgg
    44941 attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg
    45001 atgctcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg
    45061 cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt
    45121 accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc
    45181 aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat
    45241 accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc
    45301 ccctactaaa gcggcaacct tcgcggtacc aatttccagt cctactacca gttttctgtc
    45361 cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct
    45421 ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta
    45481 atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc tacaaagcga
    45541 accaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc
    45601 aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc
    45661 agcatctgcc ccatttcgcg atagccctgc aacacttcat tagcgctgcc ttccgggcca
    45721 tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa ggtgtttcct
    45781 tccgcgtcta ccatgtgttg atcattccac cgcgcaatcg gcacatattc aaccagatga
    45841 atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt
    45901 tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc cggctcaccc
    45961 aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac
    46021 accagctttg agagcggcag gcgttgcgca tcttccatcc agctcaacac gacccagccg
    46081 ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca
    46141 ttattgcggc gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac
    46201 atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg
    46261 acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag
    46321 cagataaaac tgtccatcac tgtccagcat aacgtcaata cgtccccatc ctttgcaacc
    46381 taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcttccag
    46441 acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa
    46501 ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt
    46561 gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa
    46621 tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca cactggaacc
    46681 ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt
    46741 atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg cgaccggtaa
    46801 accggcacct tgccacagaa gtttgctgcg tagtttatct attgaaagcg cagatgccat
    46861 cactccgctg ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtaccatc
    46921 ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg acttcagttg
    46981 cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt cacgcagccc
    47041 ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag tcccgcccaa
    47101 caggaccgcg attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga
    47161 tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg agaatcaggt
    47221 cgttaccggt taataccggt gccagcatct cggctacctg cgcagggtcc ggcaccagaa
    47281 tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg
    47341 gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca
    47401 gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc ggctgaaaca
    47461 gcattaccag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg gcgtcaactt
    47521 ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg
    47581 gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg aagctctcca
    47641 gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca accgcagctg
    47701 cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc ggctctttgt
    47761 cctgacgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca
    47821 cgtcggcatc ttcgctgaag ccgtaagttg tggtctgacg tcccacacgc ggtaacaatt
    47881 cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaaaggc aggttgtgca
    47941 gaaaattaat aaaggtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt
    48001 cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac
    48061 tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg
    48121 cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg
    48181 aaaccatcgc ggtagttgtc gttttgccgt gcgttcctgc aatggcgatg ccatgacgaa
    48241 aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag
    48301 ctgcgacaat ttccgggttg tcggcagaaa tggcgctgga aacaacgacc acgctggcat
    48361 cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc acccagattc agtaactgct
    48421 gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa
    48481 cttcggcaat accgcccata ccggcaccac caatgccgac aaaatgtatg tgccgaacgc
    48541 gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg
    48601 ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc
    48661 ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg
    48721 ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc
    48781 cacgctaagc tgtggctgct caataatttt ggctgcgccc gctttttcca gcggtagcgc
    48841 attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc
    48901 cgcggcgatt tcactcacag ttaaagcacc ggagcggcaa acgacgacat ccgcccacgc
    48961 atacgccgcc gccatatcat caataaattc agtcacttta tgctgcggtt gccccgcttc
    49021 ggcatacgcc tgttcaacgg attgttgcga acctttgccg ctctgatgcc agatagtgac
    49081 tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg
    49141 ggaaccaccc actaccaaca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg
    49201 caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa
    49261 agcgcctgga aacgcctgca tcactttggt agcaatcttc gccagccatt tattggttaa
    49321 gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag
    49381 accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt tgtacgcttt
    49441 cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag ctatcagtgc
    49501 ttttatacct tttccacgca aaccagagat acgaatgaaa tcaatttcga tgccatgttt
    49561 tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg
    49621 agccattaga tagtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc
    49681 catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct
    49741 ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc
    49801 agtaagctcg aaccaccgta actgattagc ggcaatgtca gacctttggt cggtaacatc
    49861 cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatagaacag
    49921 gcgagaaaac cggaaaaacg gtggtcaatt tctaatgctt tgcggccaat cgacatcgca
    49981 cgaaaagcga cgaagaatac cattaaaagt gctagtacca caccgacata acctaattct
    50041 tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt
    50101 accgaattac ctaaaccttg cccccaaagt tcgccgcgac caaacgccat cagcgattgc
    50161 gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg
    50221 cggatacggt acggttcggc gaggatcagc aacacaaccg ctgaaatgcc cataccgata
    50281 atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca
    50341 aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga
    50401 atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc
    50461 accagatagt tggcgatata gcaaaacagc gacagttttg tcagctccgc aggctggata
    50521 cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa cagcgctacc cactaccagc
    50581 acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac
    50641 tccatcggca gacgcagcgt aatgatcgcc agaataaacg ccaaaatcag atagacacca
    50701 tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc
    50761 gaggtcacca tgataaagcc aatcgccgcg aggccgaagg tcagccacag taaggtgcga
    50821 tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccctttagc
    50881 gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt caggcgaggg
    50941 agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt
    51001 caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc atatcgcccg
    51061 gctgaacacg cggagccagc aagcgcatag cctgttccat agtttcggtt tgttctgcca
    51121 cttccgggcg tagcgccgcc agctgcgcgc cgtcacgacc gaaacaatac agacgtacgt
    51181 tatcgccatt caggtaacgc accagtgggc taaagtccgc cgatttacca tcgccgccca
    51241 gcaacaaatg cagcgtgccg tctacgtgca ggccattcag cgccgcttcc gtactgccga
    51301 cgttggtcgc ttttgaatcg ttaatccagc gcacaccgtt atgctccagc accacttcaa
    51361 agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg cgcggtaacc
    51421 ctgcggcatc tgccagcgcc agcgccacca gcgcattggt gtagttatgc tgcccggaaa
    51481 gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag gtttcgccct
    51541 gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatccg
    51601 caccgcgaat cggcattgtt aaggcatcat cagcattaac cacgcaaact ttcgcgtttt
    51661 cgtaaatgcg cagttttgct gcacgatact gttgtaaacc aaacgggtag cgatccatat
    51721 gatcttcagt cacgttcaga atggtcgctg ctaccgcctg taagctggag gtggtttcca
    51781 gctggaagct cgacagttcc agcacgtaca gttcacactc atcatccagt agcatcaacg
    51841 caggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac
    51901 ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca atcggtgctt
    51961 gtgcttcgcg acaaaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag
    52021 cgcttaagga tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca
    52081 tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt aatttatcca
    52141 ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa
    52201 agtccacgca ggaaagcccg gtgaggccca ggccgataat gacaacattt ttaccctggt
    52261 aatcagccat gattaacgta ccttcagcgt tgccagacca atcaggacca gcatcagcga
    52321 aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg
    52381 atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag
    52441 gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg
    52501 acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac cgacatcgcc
    52561 cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg ccccgactat
    52621 cgcggtacag acaataacca gttccccggc gtgtcgcaga tacggtatgt gtaaatagct
    52681 ggcaaagttc atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac
    52741 ggtcggcata attgccagac cgtcaagacc atcggtcagg tttaccgcgt tgccagtacc
    52801 cacaatgacg aagtaagcca gcagaatgta gaacagcccc agttgcggca tcacatcttt
    52861 aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acaaagcgaa
    52921 agcaacaccc agcgcaatga ccgacatcca gaaatatttc caacgagcga tcaacccttt
    52981 ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac cgtaacctac
    53041 cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca gcagcacgga
    53101 gatcacaatc gctgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa
    53161 gtgtgattca ggaccgtcgt tacgcactac ctgaccaaag gaaagttttt gcaaatgagc
    53221 aatcatgcgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc
    53281 gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc
    53341 cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcagca
    53401 ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga
    53461 cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc ggtgctgata
    53521 gcatggcttt gtttacccat gcttaacacg cggtcaatac cagcagcttt tgccgcctca
    53581 cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc
    53641 agcacgcggt agcccggcat ttcagccagt acctgaacag ctgcggtcat tgaaccgaca
    53701 ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat ggggaacaga
    53761 cggccaggaa cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc
    53821 acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac
    53881 ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccat accgtgcgag
    53941 gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac
    54001 actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata
    54061 atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc
    54121 gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgccgcttcc
    54181 gggcgagtca gactcacagt ccaggcaatc tcgccctgat ggttcgcgcc aagttcaata
    54241 actgcgtaat cgtattccgg cgttaagcgc aacagcgtca tcggtacacc gatgtcgttg
    54301 ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc
    54361 tctttaacgg aggttttgcc ggaggacccc gtcagagcaa ccacgcgcgc cggaacttgc
    54421 tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc
    54481 ggcaggtcga tgtccagcgg acggctaacc agtagtgcgc ctgcgccgcc agctttcgcc
    54541 tggtcggcaa aatcgtgggc atcaaaacgt tcacctttca gggcaacaaa caggcagccc
    54601 ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc accttgcagt
    54661 tcaccgttga gaatatcggt aagttggcta agggttacgc taatcatgca atccccccca
    54721 gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt
    54781 aatcctcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc tgcataacgg
    54841 cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc
    54901 ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca
    54961 ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc ttacctttat
    55021 cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc aggcgcgccg
    55081 cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt
    55141 tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt
    55201 tcagcagatc agccagtgga tagcccagcg ccagcagtgt tgccagcgcg agcaacaggt
    55261 tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc cagcttgaac
    55321 taaagcgaat ggtcgcgccg ctgtcgtgat agttcacttc ggtcgctttc aaccagcgtc
    55381 cgtgacagtt cggattgata tgatcttcca ttgataccgc aaccgcgtcc ggcagttttg
    55441 ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgtt
    55501 cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga taatcaaggt
    55561 gatcgcggct taagttggta aagaccgacg ccggaaattt caatgccgcc acacggtgct
    55621 gtaccagccc gtgggaggaa acttccattg cgcaaaaagt cgcgccctga tccaccagcc
    55681 ccgccagctc atgctgaaca tcaactgccg aaccggtagt attttctgtc gggatcactt
    55741 tccccagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc
    55801 tccactgcgc cagaagctgg gtcgtcgtgg ttttgccgtt ggtgcccgtt acgcctacga
    55861 gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt
    55921 tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg
    55981 cctcatcttt cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat
    56041 atcgacgccc gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca
    56101 cacagctgtc gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag
    56161 caagaaggtc gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat
    56221 tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg
    56281 cccatgatgg caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc
    56341 ggatcgttga taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg
    56401 gtataagcaa tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg
    56461 gttttaatgg cgatacgata gcctttaatc gccgccttca cgccgccgcc gcctggtagc
    56521 gccacgcttt ccatcatatg caccacggtg cgaacaatgg attccgggaa gacacgttca
    56581 ccgggaaccg gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg
    56641 atagttgcgt agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag
    56701 aaggtggccc tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct
    56761 ccgaccaacc ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac
    56821 gctgaggacg gcatcgttaa cgccagcttg gaaacaccga cgttactcga cttctgtaat
    56881 accccggtca gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt
    56941 cgataaggaa tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc
    57001 atcaccacca tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta
    57061 cgcatcgact ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc
    57121 atcgccagca cttcacctgt gttgacatcc accagcacgg cgctaccaga ttcagccttg
    57181 ttaaaggcca ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata
    57241 ctcagcgcca ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga
    57301 ccatagcggt ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa
    57361 ctcttctcaa cgccctcaat cccttgacta tcgacgttag taaagccgat gaggtgagca
    57421 gtcacttcgc cggacggata gtaacggcga gactcttcac gcagatgaat ccccggcagt
    57481 ttcagttttt tgatgtagtc cgccatgtca gagtttacct gacgcgccag ataaataaag
    57541 cgccctttcg ggttggcgtt aatgcgggca gaaagctgat ccagcggaat attgagcgcg
    57601 ttagccagcg ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc
    57661 gggtcagccc aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca
    57721 gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct
    57781 ttcaccagca tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc
    57841 agcgccagga gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt
    57901 tcttcctgac gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct
    57961 tatttttgca ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc
    58021 gtggcgatcc tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg
    58081 cgccattcaa tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt
    58141 aaacgggtat ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac
    58201 aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca
    58261 tggcgctcgt ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg
    58321 atcatgcgtt cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag
    58381 ccacctcttc ttcgcccggc attaacttgc ctagtgctcg cagctgacgg ccacccagtt
    58441 ttttgagctg ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt
    58501 cacgcataaa acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc
    58561 gcccacccgg ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt
    58621 cactgtttac ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat
    58681 ctttcaccgg cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct
    58741 gttcgcggtt acgctcgaca atggcgcggg caatgcgttt ggcaaaatgc tcttcaccat
    58801 aggttttcaa tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg
    58861 actgcccacg ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc
    58921 cgcgttcagc atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga
    58981 tcttgccaat aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga
    59041 tgatggagaa gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat
    59101 cgatcgccag caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac
    59161 cgcgaccaaa agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg
    59221 cttcatccag cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc
    59281 tgcagtcgct ccgataagtc tccggtagcc aactgctctg cgtcgatatc ttccttgacc
    59341 tgttgatgcc aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc
    59401 acttcttttg tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg
    59461 ccatccatct gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc
    59521 gggttcatgc tcaacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg
    59581 taaagcagca ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc
    59641 tcaagcagct gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg
    59701 actaacgttg ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat
    59761 ttcccactga aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat
//
LOCUS       DAJTUN010000006        59795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTUN010000006 DAJTUN010000000
VERSION     DAJTUN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.006"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1380
                     /gene="hrpB"
                     /locus_tag="OO470_000356"
     CDS             <1..1380
                     /gene="hrpB"
                     /locus_tag="OO470_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6249734.1"
                     /translation="EILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRLLQ
                     MLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMGNS
                     DLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDGRY
                     QLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQLVQ
                     QSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVLNW
                     TAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLDIY
                     QALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPT
                     IAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPANTA
                     PTRRTKKYS"
     gene            1576..4110
                     /gene="mrcB"
                     /locus_tag="OO470_000357"
     CDS             1576..4110
                     /gene="mrcB"
                     /locus_tag="OO470_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6249735.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGGQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            4330..6573
                     /gene="fhuA"
                     /locus_tag="OO470_000358"
     CDS             4330..6573
                     /gene="fhuA"
                     /locus_tag="OO470_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6249736.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            6624..7421
                     /gene="fhuC"
                     /locus_tag="OO470_000359"
     CDS             6624..7421
                     /gene="fhuC"
                     /locus_tag="OO470_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6249737.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            7421..8311
                     /gene="fhuD"
                     /locus_tag="OO470_000360"
     CDS             7421..8311
                     /gene="fhuD"
                     /locus_tag="OO470_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6249738.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            8308..10290
                     /gene="fhuB"
                     /locus_tag="OO470_000361"
     CDS             8308..10290
                     /gene="fhuB"
                     /locus_tag="OO470_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6249739.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFAVAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(10325..11605)
                     /gene="hemL"
                     /locus_tag="OO470_000362"
     CDS             complement(10325..11605)
                     /gene="hemL"
                     /locus_tag="OO470_000362"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6249740.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            11830..13251
                     /gene="clcA"
                     /locus_tag="OO470_000363"
     CDS             11830..13251
                     /gene="clcA"
                     /locus_tag="OO470_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6249741.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            13275..13340
                     /locus_tag="OO470_000364"
     CDS             13275..13340
                     /locus_tag="OO470_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249742.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            13333..13677
                     /gene="erpA"
                     /locus_tag="OO470_000365"
     CDS             13333..13677
                     /gene="erpA"
                     /locus_tag="OO470_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6249743.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(13724..14347)
                     /locus_tag="OO470_000366"
     CDS             complement(13724..14347)
                     /locus_tag="OO470_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6249744.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(14385..15185)
                     /gene="btuF"
                     /locus_tag="OO470_000367"
     CDS             complement(14385..15185)
                     /gene="btuF"
                     /locus_tag="OO470_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6249745.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(15178..15876)
                     /gene="mtnN"
                     /locus_tag="OO470_000368"
     CDS             complement(15178..15876)
                     /gene="mtnN"
                     /locus_tag="OO470_000368"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6249746.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            15960..17477
                     /gene="dgt"
                     /locus_tag="OO470_000369"
     CDS             15960..17477
                     /gene="dgt"
                     /locus_tag="OO470_000369"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6249747.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            17607..19031
                     /gene="degP"
                     /locus_tag="OO470_000370"
     CDS             17607..19031
                     /gene="degP"
                     /locus_tag="OO470_000370"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6249748.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            19186..20342
                     /gene="cdaR"
                     /locus_tag="OO470_000371"
                     /pseudo
     CDS             19186..20342
                     /gene="cdaR"
                     /locus_tag="OO470_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(20431..20817)
                     /locus_tag="OO470_000372"
     CDS             complement(20431..20817)
                     /locus_tag="OO470_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6249749.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(21131..21955)
                     /gene="dapD"
                     /locus_tag="OO470_000373"
     CDS             complement(21131..21955)
                     /gene="dapD"
                     /locus_tag="OO470_000373"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6249750.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(21986..24658)
                     /gene="glnD"
                     /locus_tag="OO470_000374"
     CDS             complement(21986..24658)
                     /gene="glnD"
                     /locus_tag="OO470_000374"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6249751.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(24720..25514)
                     /gene="map"
                     /locus_tag="OO470_000375"
     CDS             complement(24720..25514)
                     /gene="map"
                     /locus_tag="OO470_000375"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6249752.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            25882..26607
                     /gene="rpsB"
                     /locus_tag="OO470_000376"
     CDS             25882..26607
                     /gene="rpsB"
                     /locus_tag="OO470_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6249753.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            26865..27716
                     /gene="tsf"
                     /locus_tag="OO470_000377"
     CDS             26865..27716
                     /gene="tsf"
                     /locus_tag="OO470_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6249754.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            27863..28588
                     /gene="pyrH"
                     /locus_tag="OO470_000378"
     CDS             27863..28588
                     /gene="pyrH"
                     /locus_tag="OO470_000378"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6249755.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            28880..29437
                     /gene="frr"
                     /locus_tag="OO470_000379"
     CDS             28880..29437
                     /gene="frr"
                     /locus_tag="OO470_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6249756.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            29529..30725
                     /gene="ispC"
                     /locus_tag="OO470_000380"
     CDS             29529..30725
                     /gene="ispC"
                     /locus_tag="OO470_000380"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6249757.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            30914..31672
                     /gene="ispU"
                     /locus_tag="OO470_000381"
                     /gene_synonym="uppS"
     CDS             30914..31672
                     /gene="ispU"
                     /locus_tag="OO470_000381"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6249758.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            31685..32542
                     /gene="cdsA"
                     /locus_tag="OO470_000382"
     CDS             31685..32542
                     /gene="cdsA"
                     /locus_tag="OO470_000382"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6249759.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            32554..33906
                     /gene="rseP"
                     /locus_tag="OO470_000383"
     CDS             32554..33906
                     /gene="rseP"
                     /locus_tag="OO470_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6249760.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            33936..36368
                     /gene="bamA"
                     /locus_tag="OO470_000384"
     CDS             33936..36368
                     /gene="bamA"
                     /locus_tag="OO470_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6249761.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            36490..36975
                     /gene="skp"
                     /locus_tag="OO470_000385"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             36490..36975
                     /gene="skp"
                     /locus_tag="OO470_000385"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6249762.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            36979..38004
                     /gene="lpxD"
                     /locus_tag="OO470_000386"
     CDS             36979..38004
                     /gene="lpxD"
                     /locus_tag="OO470_000386"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6249763.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            38109..38564
                     /gene="fabZ"
                     /locus_tag="OO470_000387"
     CDS             38109..38564
                     /gene="fabZ"
                     /locus_tag="OO470_000387"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6249764.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            38568..39356
                     /gene="lpxA"
                     /locus_tag="OO470_000388"
     CDS             38568..39356
                     /gene="lpxA"
                     /locus_tag="OO470_000388"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6249765.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            39356..40504
                     /gene="lpxB"
                     /locus_tag="OO470_000389"
     CDS             39356..40504
                     /gene="lpxB"
                     /locus_tag="OO470_000389"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6249766.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            40501..41097
                     /gene="rnhB"
                     /locus_tag="OO470_000390"
     CDS             40501..41097
                     /gene="rnhB"
                     /locus_tag="OO470_000390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6249767.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            41134..44616
                     /gene="dnaE"
                     /locus_tag="OO470_000391"
     CDS             41134..44616
                     /gene="dnaE"
                     /locus_tag="OO470_000391"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6249768.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            44629..45588
                     /gene="accA"
                     /locus_tag="OO470_000392"
     CDS             44629..45588
                     /gene="accA"
                     /locus_tag="OO470_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6249769.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            45687..47828
                     /gene="ldcC"
                     /locus_tag="OO470_000393"
     CDS             45687..47828
                     /gene="ldcC"
                     /locus_tag="OO470_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6249770.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            47885..48274
                     /locus_tag="OO470_000394"
     CDS             47885..48274
                     /locus_tag="OO470_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6249771.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            48339..49637
                     /gene="tilS"
                     /locus_tag="OO470_000395"
     CDS             48339..49637
                     /gene="tilS"
                     /locus_tag="OO470_000395"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6249772.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(49686..49946)
                     /gene="rof"
                     /locus_tag="OO470_000396"
     CDS             complement(49686..49946)
                     /gene="rof"
                     /locus_tag="OO470_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6249773.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(49933..50133)
                     /locus_tag="OO470_000397"
     CDS             complement(49933..50133)
                     /locus_tag="OO470_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6249774.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            50299..50844
                     /locus_tag="OO470_000398"
     CDS             50299..50844
                     /locus_tag="OO470_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6249775.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            50841..51263
                     /gene="arfB"
                     /locus_tag="OO470_000399"
     CDS             50841..51263
                     /gene="arfB"
                     /locus_tag="OO470_000399"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6249776.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            51277..51987
                     /gene="nlpE"
                     /locus_tag="OO470_000400"
                     /gene_synonym="cutF"
     CDS             51277..51987
                     /gene="nlpE"
                     /locus_tag="OO470_000400"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6249777.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(52142..52966)
                     /locus_tag="OO470_000401"
     CDS             complement(52142..52966)
                     /locus_tag="OO470_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6249778.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(53019..54737)
                     /gene="proS"
                     /locus_tag="OO470_000402"
     CDS             complement(53019..54737)
                     /gene="proS"
                     /locus_tag="OO470_000402"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6249779.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(54848..55555)
                     /gene="tsaA"
                     /locus_tag="OO470_000403"
     CDS             complement(54848..55555)
                     /gene="tsaA"
                     /locus_tag="OO470_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6249780.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(55552..55956)
                     /gene="rcsF"
                     /locus_tag="OO470_000404"
     CDS             complement(55552..55956)
                     /gene="rcsF"
                     /locus_tag="OO470_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6249781.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(56074..56889)
                     /gene="metQ"
                     /locus_tag="OO470_000405"
     CDS             complement(56074..56889)
                     /gene="metQ"
                     /locus_tag="OO470_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6249782.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(56929..57582)
                     /gene="metI"
                     /locus_tag="OO470_000406"
     CDS             complement(56929..57582)
                     /gene="metI"
                     /locus_tag="OO470_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6249783.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(57575..58606)
                     /gene="metN"
                     /locus_tag="OO470_000407"
     CDS             complement(57575..58606)
                     /gene="metN"
                     /locus_tag="OO470_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6249784.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            58794..59369
                     /gene="gmhB"
                     /locus_tag="OO470_000408"
     CDS             58794..59369
                     /gene="gmhB"
                     /locus_tag="OO470_000408"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6249785.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            59733..>59795
                     /locus_tag="OO470_000409"
     rRNA            59733..>59795
                     /locus_tag="OO470_000409"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        14202 a        14743 c        16489 g        14361 t
ORIGIN      
        1 gagatcttac aaagcgatct ttccggtttg ctgatggaat tactgcaatg gggatgcagc
       61 gatccggcgc agatgagctg gctggatcaa ccgccaacgg tgaatctact ggccgcgaaa
      121 cgcctgttac aaatgctggg ggcgctggag ggtgaacggc ttagtgcgca agggcaaaaa
      181 atggcagcgc tgggtaacga tccgcgttta gcggcaatgc tggttaacgc aaagagcgat
      241 gacgaagctg ctaccgcggc aaaaattgcc gccattctcg aagagccgcc acggatgggc
      301 aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag cctggcagca acgtagtcag
      361 caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg
      421 ctacttgccg gggcgtttgc cgatcgcatt gctcatcgcc gtgggcaaga tggacgctat
      481 caactggcaa acagcatggg agcgatgctc gatgccgacg acgcgctaag ccgccacgaa
      541 tggttgatcg caccgttatt attgcagggc agcgcctcgc cggatgcgcg gattttactg
      601 gcgctaccgg tcgatattga tgaattagta caacgctgtc cgcagctggt gcagcaatct
      661 gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag cctggcgtcg gctacaaatc
      721 ggtcagttga cggtgaaagt gcagccgctg gcgaaaccgt cagaagacga gttgcatcag
      781 gcgatgctta atggcatccg tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg
      841 gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt tgccggaata tgactggcca
      901 gcggttgatg atgaaagttt gttggcgacg ctggaaacgt ggctgctgcc acatatgacc
      961 ggcgtacatt cactacgcgg cctgaaatca ctcgacattt atcaggcact acgcggatta
     1021 cttgattggg gaatgcagca acgtctggat agtgaattgc ctgcgcatta cactgtgccg
     1081 acgggaagcc ggatcgccat tcgttatcat gaagataacc cgcccgcgct ggcggtgaga
     1141 atgcaggaga tgtttggtga ggccactaat ccgacgatcg cccaggggcg cgtgccgctg
     1201 gtgctgaagt tgctttcccc tgcccaaagg ccgctgcaaa tcacgcgtga tttgggggcc
     1261 ttctggaaag gggcgtatcg tgaggtgcaa aaggagatga aagggcgtta tcccaaacac
     1321 gtctggccgg acgacccggc aaacacggca ccgacgcgac ggacgaaaaa gtattcgtaa
     1381 ggttggtttt ctccctctcc ctgtgggaga gggccggggt gagggcatca gcgcgcacgt
     1441 tcaccctcac cctaaccctc tccctcaagg gagaggggac cgatcgagcg caaattttga
     1501 gagatatctt cttctgtctt gtaacagaag aacagaaaat cgggcttttg cgcctgaata
     1561 ttgcggagaa aaagcatggc cgggaatgac cgcgagccaa ttggacgcaa agggaaaccg
     1621 acgcgtccgg tcaaacaaaa ggtaagccgt cgtcgttacg aagatgacga tgattacgac
     1681 gattatgatg actatgagga tgaagaaccg atgccgcgca aaggtaaggg caaaggcaaa
     1741 gggcgtaagc ctcgtggcaa acgcggctgg ctatggctac tgctaaaact ggctatcgtt
     1801 tttgccgtgc tgatcgccat ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt
     1861 gatggcaagg tctggcaact gcccgcggca gtttatggcc gaatggtcaa ccttgaacca
     1921 gatatgacca tcagcaagaa cgaaatggtg aagctgctgg aggcaacaca gtatcgtcag
     1981 gtgtcgaaaa tgacccgtcc tggcgaattt accgtgcagg ccaacagcat tgagatgatc
     2041 cgccgtccgt ttgatttccc ggacagtaaa gaagggcagg tgcgtgcgcg tctgaccttt
     2101 gatggtgatc atctggcgac catcgtcaat atggagaaca accgtcagtt cggtttcttc
     2161 cgtcttgatc cgcgtctgat caccatgatc tcctcgccaa acggtgagca gcgtttgttt
     2221 gtgccgcgca gtggtttccc tgatttgctg gtggatactt tgctggcgac agaagaccgt
     2281 catttttacg agcatgatgg aatcagtctc tactccattg ggcgtgcggt gctggcaaac
     2341 ctgaccgcag gtcgcacggt gcagggtgcg agtacgctga cgcaacagct ggtgaaaaac
     2401 ctgttcctct ccagcgagcg ttcttactgg cgtaaagcga acgaagctta catggcgctg
     2461 atcatggacg cgcgttacag caaagaccgt attcttgagc tgtatatgaa cgaggtgtat
     2521 ctcggtcaga gcggcgacaa cgaaatccgc ggcttcccgc tggcaagctt gtattacttt
     2581 ggtcgcccgg tagaagagct aagcctcgac cagcaggcgc tgttagtcgg tatggtgaaa
     2641 ggggcgtcca tctacaaccc gtggcgtaac ccgaaactgg cgctggagcg acgtaatctg
     2701 gtgctgcgtc tgctgcaaca gcaacagatt attgatcaag aactctatga catgttgagt
     2761 gcccgtccgc tggggggtca gccgcgtggt ggggtgatct ctcctcagcc agcctttatg
     2821 cagctggtgc gtcaggagtt gcaggcaaaa ctgggcgata aggtaaaaga tctctccggc
     2881 gtgaagatct tcactacctt tgactcggtg gcccaggacg cggcagaaaa agccgccgtg
     2941 gaaggcattc cggcactgaa gaaacagcgt aagttgagcg atcttgaaac tgcgattgtg
     3001 gtcgtcgacc gctttagtgg tgaagttcgt gcgatggtcg gaggttctga gccgcagttt
     3061 gcgggctaca accgtgcgat gcaggcgcgt cgttcgattg gttcccttgc aaaaccagcg
     3121 acttatctga cggctttaag ccagccgaaa atctatcgcc tgaacacgtg gattgccgat
     3181 gcgccaatcg cgctgcgtca gccgaatggc caggtctggt caccgcagaa cgatgaccgt
     3241 cgttatagcg aaagcggcag agtgatgctg gtggatgcgt tgacacgttc gatgaacgtg
     3301 ccgacggtaa atctggggat ggcgctgggg ctgcctgcgg ttacggagac ctggatcaaa
     3361 ctgggcgtac cgaaagatca gttgcatccg gttccggcaa tgctgctggg ggcgttgaac
     3421 ttaacgccaa tcgaagtggc gcaggcattc cagaccatcg ccagcggtgg taaccgtgca
     3481 ccgctttctg cactgcgttc ggtaatcgcg gaagatggta aggtgctgta tcagagcttc
     3541 ccgcaggcag aacgcgctgt tccggcgcag gcggcgtatc tgacactatg gaccatgcag
     3601 caggtggtac aacgtggtac gggtcgtcag cttggggcga aatacccgaa cctgcatctg
     3661 gcagggaaaa cagggactac caacaataac gtagatacct ggtttgcagg cattgacggc
     3721 agcacggtga ccatcacctg ggtaggccgt gataacaacc agccgaccaa actgtatgga
     3781 gccagcgggg caatgtcgat ttatcagcgt tatctggcta accagacgcc aacgccgctg
     3841 aatcttgttc cgccagaaga tattgcagat atgggcgtgg actacgacgg caactttgtt
     3901 tgcagcggtg gcatgcgtgt cttgccggtc tggaccagcg atccgcaatc gctgtgccag
     3961 cagagcgaga tgcagcagca gccgtcaggc aatccgtttg atcagtcttc tcagccgcag
     4021 caacagccgc aacagcaacc tgctcagcaa gagcagaaag acagcgacgg tgtagccggt
     4081 tggatcaagg atatgtttgg tagtaattaa catctaagcg tgaaataccg gatggcgagt
     4141 tgccatccgg taaaataaca tcccatctaa gatattaacc ctttcttttc atctggttgt
     4201 ttattaaccc ttcaggaacg ctcagattgc gtaccgcttg cgaacccgcc agcgtttcga
     4261 atattatctt atctttataa taatcattct cgtttacgtt attattcact ttacatcaga
     4321 gatataccaa tggcgcgttc caaaactgct cagccaaaac actcactgcg taaaatcgca
     4381 gttgtagtag ccacagcggt tagcggcatg tctgtttatg cacaggcagc ggttgaaccg
     4441 aaagaagaca ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc atgggggcct
     4501 gctgcaacta ttgcggcgcg gcagtcagct accggcacta aaaccgatac gccgattcaa
     4561 aaagtgccac agtctatttc tgttgtgacc gccgaagaga tggcgctgca tcagccgaag
     4621 tcggtaaaag aagcgcttag ctacacgccg ggtgtctctg ttggtacgcg tggcgcatcc
     4681 aacacctatg accacctgat cattcgcggt tttgcggcag aaggccaaag ccaaaataac
     4741 tatctgaatg gcctgaagtt gcagggcaac ttctataacg atgcggtcat tgacccgtat
     4801 atgctggaac gcgctgaaat tatgcgtggc ccggtttccg tgctttacgg taaaagcagt
     4861 cctggcggtc tgttgaatat ggtcagcaag cgtccgacca ccgaaccgct gaaagaagtt
     4921 cagtttaaag ccggtactga cagcctgttc cagactggtt ttgactttag cgatgcgttg
     4981 gatgatgacg gtgtttactc ttatcgcctg accggtcttg cgcgttctgc caatgcccag
     5041 cagaaagggt cagaagagca gcgttatgct attgcaccgg cgttcacctg gcgtccggat
     5101 gataaaacca atttcacctt cctttcttac ttccagaacg agccggaaac cggttattac
     5161 ggctggttgc cgaaagaggg aaccgttgag ccgctgccga acggtaagcg tctgccgaca
     5221 gactttaatg aaggggcgaa gaacaacacc tattctcgta atgagaagat ggtcggctac
     5281 agcttcgatc acgaatttaa cgacaccttt actgtgcgtc agaacctgcg ctttgctgaa
     5341 aacaaaacct cgcaaaacag cgtttatggt tacggcgtct gctccgatcc ggcgaatgct
     5401 tacagcaaac agtgtgcggc attagcgcca gcggataaag gccattatct ggcacgtaaa
     5461 tacgtcgttg atgatgagaa gctgcaaaac ttctccgttg atacccagtt gcagagcaag
     5521 tttgccactg gcgatatcga ccacaccctg ctgaccggtg tcgactttat gcgtatgcgt
     5581 aatgacatca acgcctggtt tggttacgac gactctgtgc cactgctcaa tctgtacaat
     5641 ccggtgaatc ccgatttcga cttcaatgcc aaagatccgg caaactccgg cccttaccgc
     5701 attctgaata aacagaaaca aacgggcgtt tatgttcagg atcaggcgca gtgggataaa
     5761 gtgctggtca ccctaggcgg tcgttatgac tgggcagatc aagaatctct taaccgcgtt
     5821 gctgggacga ccgataaacg tgatgacaaa cagtttacct ggcgtggtgg tgttaactac
     5881 ctgtttgata atggtgtaac accttacttc agctatagcg aatcgtttga accttcttcg
     5941 caagttggga aggatggtaa tattttcgca ccgtctaaag gtaagcagta tgaagtcggc
     6001 gtgaaatatg taccggaaga tcgtccgatt gtagttactg gtgccgtgta taatctcact
     6061 aaaaccaaca acctgatggc ggaccctgag ggttccttct tctcggttga aggtggcgag
     6121 atccgcgcac gtggcgtaga aatcgaagcg aaagcggcgc tgtcggcgag tgttaacgta
     6181 gtcggttctt atacttacac cgatgcggaa tacaccaccg atactaccta taaaggcaat
     6241 acgcctgcac aggtgccaaa acacatggct tcgctgtggg ctgactatac cttctttgac
     6301 ggtccgcttt caggtctgac gctgggcacc ggtggtcgtt atactggctc cagctatggt
     6361 gatccggcta actcctttaa agtgggaagt tatacggtcg tggatgcgtt agtgcgttat
     6421 gatctggcgc gagtcggcat ggcgggctcc aacgtggcgc tgcatgttaa caacctgttc
     6481 gatcgtgaat acgtcgccag ctgctttaac acttatggct gcttctgggg cgcagaacgt
     6541 caggtcgttg caaccgcaac ctttcgtttc taatttctct tttggggcac ggatttccgt
     6601 gcccatttca caagttggct gttatgcagg aatacacgaa tcattccgat accacttttg
     6661 cactgcgtaa tatctccttt cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa
     6721 cctttcctgc cgggaaagtg accggtctga ttggtcacaa cggttctggt aaatccactc
     6781 tgctcaaaat gcttggtcgt catcagcctc cgtcagaagg ggagattctt cttgatgccc
     6841 agccgctgga aagctggagc agcaaagcgt ttgcccgcaa agtggcttat ttgccgcagc
     6901 agcttcctcc ggcagaaggg atgaccgtgc gtgaactggt ggcgattggt cgttacccgt
     6961 ggcatggcgc gctggggcgc tttggtgcgg cagatcgcga aaaggtcgag gaagctatct
     7021 cgctggttgg cttaaaaccg ctggcgcatc ggctggtcga tagtctctct ggcggcgaac
     7081 gtcagcgggc gtggatcgcc atgctggtgg cgcaggatag ccgttgtctg ttgctcgacg
     7141 aaccgacttc agcgctggat atcgcccacc aggttgatgt gctggcgctg gtgcaccgtt
     7201 taagtcagga acgtggcctg acggtcattg ccgtgttgca cgatatcaat atggcggcac
     7261 gctactgtga ttatctggtc gccctgcgca gtggtgaaat gattgctcag ggaacgcctg
     7321 cggaaattat gcgcggcgaa accctcgaaa tgatttatgg catcccgatg ggtattttgc
     7381 cgcatccggc gggtgctgca cctgtgagtt ttgtttattg atgagcggct tacctcttat
     7441 ttcgcgccgt cgactgttaa cggcgatggc gctttctccg ttgttatggc agatgaatac
     7501 cgcccacgcg gcggctattg accccaatcg tattgtggcg ctggagtggt tgccggtgga
     7561 attactgctg gcgctcggca tcgtgcctta cggcgtggcg gataccatca actatcgcct
     7621 gtgggtcagc gaaccaccat tgccggactc agtgattgac gtcggtttgc gtacagaacc
     7681 taaccttgaa ctgctgaccg aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg
     7741 cccttcatca gaaatgctgg cacgtattgc gccgggtcgc ggatttaact tcagtgacgg
     7801 caaacatccg ttggcgatgg cgcgtaaatc gctgacggaa atggcagatt tacttaacct
     7861 gcaaagcgca gcggaaacgc atttagcgca atacgaagac tttatccgca gcatgaaacc
     7921 ccgctttgtg aagcgtggtg cacgcccgtt actgctgacg acgcttatcg atccgcgcca
     7981 tatgctggtc ttcggtccaa acagcttgtt ccaggaaatt ctcgatgagt acggcatccc
     8041 aaatgcctgg caaggggaaa ccaacttctg gggcagtacc gccgtcagta tcgatcgtct
     8101 ggcagcgtat aaagacgttg atgtgctctg ttttgatcac gacaacagca aagacatgga
     8161 tgcgctaatg gcaaccccgc tgtggcaggc catgccgttt gtccgtacag ggcgctttca
     8221 gcgcgtaccc gcagtctggt tttatggtgc aacgctttcg gcaatgcact ttgtgcgtgt
     8281 tctggataac gccatcggag gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta
     8341 ttgctggcgc tgttagtgat tgtcgctacg gcgctcacct ggatgaactt ctcacaggcg
     8401 ctgccgcgca gtcagtgggc gcaggccgcc tggtcgccgg atattgacgt catcgagcag
     8461 atgatttttc actacagctt gttgccgcgt ctggcgattt cgctgctggt gggcgcgggc
     8521 ctggggctgg tgggcgtgct gtttcagcaa gtactgcgta acccgctggc ggaaccgacg
     8581 acgttgggcg ttgcaacagg cgcgcaactg gggattaccg tcaccacgct ctgggcgatc
     8641 cctagcgcga tggcgagcca gtttgctgcg ctggcagggg cttgcgttgt tggcttaatt
     8701 gtctttggcg tcgcgtgggg gaaacggctg tcgccggtaa cgctgatttt cgcggggttg
     8761 gtagtgagcc tttattgcgg cgcaatcaat cagttactgg ttatcttcca tcatgaccag
     8821 ttgcaaagca tgtttctgtg gagcactgga acgctgacgc aaaccgactg gggcggtgtt
     8881 gagcgtttat ggccgcagtt gctgggcggt gtgatgctga cgttgctgct acttcgtccg
     8941 ttaaccctga tggggcttga tgatggcgtg gcgcgcaatc tcgggctggc cttgtcgctt
     9001 gcgcgtctgg cggcgctgtc gctggcgatt gtcatcagtg cgctgctggt gaacgctgtg
     9061 gggattatcg gctttatcgg gttgttcgcg ccgctgctgg caaaaatgct gggggcgcgg
     9121 cgtctgctgc cacgactgat gctggcgtcg ttgattggtg cgctgatcct ctggctttcc
     9181 gatcaaatca tcctctggct gactcgcgtg tggatggaag tgtccaccgg ttcggtcact
     9241 gcgttgatcg gtgcgccgct gctactgtgg ctgctgccgc gtttacgcag cattagcgcg
     9301 ccggatatga aggttaacga tcgtgtcgcg actgaacgcc aacatgtgct ggcgtttgcc
     9361 gtcgcgggcg gcgtgctgtt gttgatggct gtggtggtgg cgctgtcgtt tggtcgtgat
     9421 gcgcacggct ggacgtgggc gagcggggcg ttgctcgagg atttaatgcc ctggcgctgg
     9481 ccgcgaatta tggcggcgct gtttgcgggc gtcatgctgg cggtagcggg ctgtattatt
     9541 cagcgactga ccggcaaccc gatggcaagc ccggaagtgc tggggattag ctccggcgcg
     9601 gcgtttggtg tggtgttaat gctgtttctg gtaccgggta atgcctttgg ctggttgtta
     9661 cccgcaggca gtctcggggc ggcggtgacg ctgttgatca ttatgatcgc cgccggacgc
     9721 ggtggatttt ccccacaccg catgttactg gcggggatgg cgttaagtac cgcgttcacc
     9781 atgcttttga tgatgttgca ggcaagtggt gacccgcgaa tggcgcaagt gctgacctgg
     9841 atttccggtt cgacctacaa cgcgaccgat gctcaggtct ggcgcaccgg aattgtgatg
     9901 gtgattttgc tggcgattac tccgctgtgc cgccgctggc tgaccatttt accgctgggt
     9961 ggtgataccg cgcgggcggt agggatggcg ctgacgccga cgcgaattgc gctgctgctg
    10021 ttagcggctt gcctgacggc gaccgcgacg atgactattg gaccgttgag ttttgttggt
    10081 ttaatggcac cgcatattgc gcggatgatg ggctttcgac ggacgatgcc acacatcgta
    10141 atttcggcgc tggtgggtgg tttactgctg gtgttcgctg actggtgtgg gcggatggtg
    10201 ctgtttccat tccagatccc ggcggggctg ctgtcgacct ttatcggtgc gccatatttt
    10261 atctatttgc tgagaaagca gagccgttaa tagaatgcct cgcccggtgg gcgaggcaaa
    10321 attatcacaa cttcgcgaac acccgacgtg cagcatcgat ggtgttattg atatcttcca
    10381 tgctgtgcgc cacagacata aagcccgctt caaacgctga cggtgccagg taaacacctt
    10441 catccagcat catatggaag aaacgcttaa aacgctcgac gtcacaggcc atcacatcct
    10501 gatagcacgt cacggactcg gcgtcggtaa agaagatacc gaacatacca ccgacgtggt
    10561 taacgaccag cggaattccc gcttcttctg ccgcttccag cagaccttct gccagacgtg
    10621 ttgtcagctc atccagcgtt tcgtgaatgc ccggctgcgc gacttcattc agacaggcga
    10681 aacccgctgc catcgcaatc gggttaccgg aaagcgtacc cgcctgatag accggacctg
    10741 tcggggccag cgcatccatt acatcacgac gaccaccgaa tgcgcctacc ggcattccac
    10801 cgccgatgat ttttccgagg caggttaaat ctggcactac ggcgaaataa tcctgtgcgc
    10861 cagctagcgc tacgcggaaa ccggtcatca cttcatcgat gatcagcaac gcgccaaatt
    10921 cgtcgcacag cgcacgcaga cctggcagga actctggcag cggcggaaca cagttcatat
    10981 tgcctgccac cggctcgacg ataatacagg caatctcttg cgggtattgc tcaaacgcgg
    11041 cgcgtacaga ggccagatcg ttataagtac aggttaaggt atgtttggcg aaatctgccg
    11101 gaacgcccgg cgagtttggc tggcctaacg tgagtgcgcc agaaccggct ttcaccagca
    11161 ggcagtcagc gtgaccgtgg taacaacctt caaatttaat gattttgtcg cgaccggtaa
    11221 aaccacgggc caggcggatg gcgctcatcg tcgcctcggt gccggagttc accatgcgca
    11281 ccatatccat ggtcgggacc agttcggtca ccagttgcgc cattttcact tccatttcgg
    11341 tcggtgcacc aaagcttaaa ccacgctcgg cggcttcaat cacggcattg cggatcgccg
    11401 gatggttatg gcccagcacc atcggccccc aggaaccgac ataatcgata taggctttgc
    11461 catcaacatc gtacagataa gcgccgtccg ctttttcgat aaacagtgga gtgccgccca
    11521 cgccagtaaa ggcgcgaaca ggggagttca caccgccagg gatcagctcg cgcgctgcgc
    11581 tgtaaagatt ttcagactta ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct
    11641 gctattccat gttattcata acaagttaaa tactcgtcaa acatcaggct gcttgtactg
    11701 gtgcaatcct gatttcttag agtataaaag ttttgtgcat ttgaaacatt acgctttgca
    11761 aaggattttc atggaacgtg cgagtaaaat gccgtcatct tatttgtatg accaataagt
    11821 gatcattgga tgaaaactga tactccctct ttagaaacac cgcaggccgc gcgcctgcga
    11881 cgcagacaac tgattcgcca acttcttgag cgcgataaaa ccccgttagc cattttgttt
    11941 atggcggcag tcgtcggcac gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc
    12001 gcctggttgc agaaccaacg tatgggggcg ctggtacata ctgctgataa ttatccgcta
    12061 ctgttaaccg tcgcttttct ctgttcggcg gtgctggcga tgttcggcta ctttttggtg
    12121 cgcaaatacg cgcccgaagc agggggatcg gggatcccgg aaattgaagg ggcgctggaa
    12181 gatcaacgtc ctgttcgctg gtggcgtgta ttgccggtga aattctttgg cgggctgggg
    12241 acactcggcg gaggcatggg gctagggcgc gaaggaccaa ccgtgcagat cggcggcaat
    12301 atcagtcgca tggtgcttga tattttccgc ctgaaaggtg atgaagctcg ccatacgctg
    12361 ctggcaaccg gtgctgctgc ggggctggct gcggccttta acgcgccgct ggcgggtatt
    12421 ttgtttatta tcgaagagat gcgtccgcag tttcgctata cgttaatttc gattaaagcg
    12481 gtatttattg gcgtcattat gtcgaccatt atgtaccgga tttttaatca tgaagttgcg
    12541 ttgattgacg tcggtaaact ttctgatgcg ccgcttaata cgctgtggct ttatctgatc
    12601 ctcggtatta tttttggcat tttcggccct atttttaata aatgggtgct ggggatgcag
    12661 gatttgctgc accgtgtgca cggcggcaat attaccaaat gggtactgat ggggggtgcg
    12721 attggcggtc tgtgtggatt gctggggttt gtggcaccag caacgtcggg cggcggtttt
    12781 aacctgattc ctatcgctac cgcggggaat ttcagcatgg gaatgctggt gtttatcttc
    12841 gtcgctcggg tcattaccac cttactctgc ttctcttccg gcgcgccggg cggtattttt
    12901 gccccgatgc tggcgctggg tactgtgctg ggaaccgctt tcggaatggt tgccgttgag
    12961 ctgtttccgc aatatcacct ggaggcgggg acgtttgcta ttgccggaat gggggcatta
    13021 ctggcggcat ctattcgcgc gccgttaacg gggatcattc tggttctgga gatgaccgat
    13081 aactaccagc tcattttgcc aatgattatt accggtcttg gcgcaacact attagcgcaa
    13141 tttaccggcg ggaaacctct atactcggcg attcttgcgc gcacgctggc aaaacaggaa
    13201 gctgagcaac tggcgcgaag caaggccgca tcagccagcg agaatacttg aacgaaatac
    13261 cagggtatta gataatggcg attattattg ggttagaatt tgcccgattg ccgatgtcgt
    13321 ttggagcaaa atatgagtga tgacgtagca ctgccgctgg agtttaccga cgcagcagcc
    13381 aacaaagtta aaagcctgat cgctgacgaa gataacccga atctgaaatt acgcgtgtat
    13441 atcaccggtg gcggttgcag cggcttccag tatggtttca cctttgatga tcaggtgaac
    13501 gaaggcgata tgaccatcga aaaacagggc gttggcctgg tggttgatcc gatgagcctg
    13561 caatatctgg tcggcggttc cgttgactac accgaaggac tggaaggttc tcgtttcatc
    13621 gtgaccaacc cgaacgcgaa aagcacctgc ggttgcggtt cttcctttag catctaatct
    13681 gttgttcatg tgccggataa actatccggc acatcttctc tcttcaacgc ccattctcat
    13741 ccagcgcaaa cgtcggtagc ttaagatgcc aacgaatagc cgccagccga atcaatagcg
    13801 tcacgaccat gcccatcata ctggctgttt ccagtggtac ggaaaatgtg taataagccg
    13861 tagcgtggac aataccgccg ataatacagg cagttgcgta gatttctgta cgtaaaatca
    13921 tggggatttc gcggaccagt acatcacgaa tgatcccgcc gccaacgcca gtaatgacgc
    13981 ccatacaaac cgcgattaac ggaccggctt ccgcattaaa ggctttattc acgccaatgc
    14041 cgacaaacac cgccagacca acggcgtcca gcaccggcaa catccatttt ggtaagcgtc
    14101 ttggctggcg caccagcacg atggtcagca tgctggtgac catggcaacg accagatcgg
    14161 tgggatcttt cacccaaaat accgggccgt gatccagcgc catgtcgcga attgttccgt
    14221 cgcctactgc ggtaaccacg cccagtacca gaacaccaaa agggtccata cgcaattttc
    14281 cggctaacaa aacgccgtag atggcaaata ccgctgtgcc gactatatcc agccaataga
    14341 cgagcattgt tcaatcccca ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa
    14401 agcgcattac agagctgttg tgctgcgagg ataatacgtg ggcttgcacg ttcaaaccag
    14461 tcactcgtga gaggaataac gggaattttg agctgttcac cccagtattg tttgatttta
    14521 ggaatttggt ccggtccgcc tgtaatgaca atagcctgtg gcgagcgtgc taacacctgt
    14581 tcgcggctaa cttgcggcca gggaacccgg ctgtctttaa agatgttttc tccgccacaa
    14641 acttcgagta cctggttctg aatcgactct tttccactgg taaatggcgg attaatgccg
    14701 aattgcagaa aaacacgttt tttaggttta tcagcatatt gcgctttcaa ttgcacgtac
    14761 tgatccagca gggattgcgc ggcttgttcg gccttgtctg gttgcggact ccagggggcc
    14821 agttgacgta acgcattggc aatttgttca atgcttgtcg catcgaccca catcactttt
    14881 attcccagcg aagccagctg gtcaacctgc cgctcggcat tacctccacg ccaggcaatc
    14941 accagatcgg gtttcagcgc gacaatgcgt tccagattca tcccctgcca ggtggaaacc
    15001 tgctcaatct tttgcgcttg tggaggatag tcggaatagc tgctgacccc aaccggcgtg
    15061 atcccggcgg caaaggcaag ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg
    15121 gcgttgagcc acagtggcgc gagaaaagac agggcgacca gcgccctgaa cagtgattta
    15181 gccatgtgcc agtttctgca ccagtgactc aaccatcagg ctggactgtt tagcggcaac
    15241 agccaggaac tcatcgaagc taagatgaga ctgttgatcg gccacgtcgg agatggcgcg
    15301 tacgacaaca aacgggacgt tgaaattgtg gcagacatgg gcgattgccg tcgcttccat
    15361 ctctacagca atggcctgtg ggaagttgtg gcggattttc gccagaccaa cagaaccgtt
    15421 gatgaaagcg tcgccgctaa caatcaggcc acgtacagcg ttaagattca gttcggcaat
    15481 gcaggcctca gcggcagcga tcagtttatc gtcagcttta aagcctgccg gacagcctgg
    15541 taactgaccg tattcataac caaatgccgt gacatccgcg tcgtgataac gcgcttcgtc
    15601 cgagacaacg atatcgccca ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt
    15661 aataatcaca tctggcttgc agtgttccaa cagcagagtg gcacccagcg ccgcagcgac
    15721 tttaccgatg cccgatttca gaagcgcaac ctcggttcca ttcagttggc cggtatagat
    15781 ttcgcaaccg ccgagactga tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac
    15841 ttcttcttcc attgcaccaa tgatgccgat tttcatagat ttactcgcga taagcccgat
    15901 ttgaaggcat agtttaccat gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca
    15961 tggcacagat tgatttccga aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg
    16021 gcgttaaaac cgaacatgag atcctgcgga tcttcgagag cgatcgcggg cgtatcatca
    16081 actctccggc aattcgtcgt ctgcaacaaa agacccaggt ttttccactg gagcgcaatg
    16141 ccgccgtgcg cacgcgtctt acccactcga tggaagtcca gcaggtggga cgctacatcg
    16201 ccaaagaaat tttaagccgt ctgaaagagc ttaaattgct ggaagcatac ggcctggatg
    16261 aactgaccgg cccctttgaa agcattgttg agatgtcatg cctgatgcac gatatcggca
    16321 atccgccgtt tggtcatttt ggcgaagcag cgataaatga ctggtttcgc caacgtttgc
    16381 acccggaaga tgccgaaagc cagcctctga ctgacgatcg ctgcagcgtg gcggcactac
    16441 gtttacggga cggggaagaa ccgcttaacg agctgcggcg caagattcgt caggacttat
    16501 gtcattttga ggggaatgca caaggcattc gtctggtgca tacattgatg cggatgaatc
    16561 tcacttgggc acaggttggc ggtattttaa aatatacccg tccggcgtgg tggcgtggcg
    16621 aaacgcctga gacacatcac tatttaatga aaaagccggg ttattatctt tctgaagaag
    16681 cctatattgc ccgcttgcgt aaagaactta atttggcgct ttacagtcgt tttccattaa
    16741 cgtggattat ggaagctgcc gacgacatct cctattgtgt ggcagacctt gaagatgcgg
    16801 tagagaaaag aatatttacc gttgagcagc tttatcatca tttgcacgaa gcgtggggcc
    16861 agcatgagaa aggttcgctc ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct
    16921 caaatagttt aagccgcagt acggaagatc agttttttat gtatttacgg gtaaacaccc
    16981 taaataaact ggtaccctac gcggcacaac gatttattga taatctgcct gcgattttcg
    17041 ccggaacgtt taatcatgca ttattggaag atgccagcga atgcagcgat cttcttaagc
    17101 tatataaaaa tgtcgctgta aaacatgtgt ttagccatcc ggatgtcgag cagcttgaat
    17161 tgcagggcta tcgggtcatt agcggattat tagagattta tcgtccttta ttaagcctgt
    17221 cgttatcaga ctttactgaa ctggtagaaa aagaacgggt gaaacgtttc cctattgagt
    17281 cgcgcttatt ccacaaactc tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta
    17341 aattaccgtc agattctcct gagtttccgc tatgggaata ttattaccgt tgccgcctgc
    17401 tgcaggatta tatcagcggt atgaccgacc tctatgcgtg ggatgaatac cgacgtctga
    17461 tggccgtaga acaataacca ggcttttgta aagacgaaca ataaattttt accttttgca
    17521 gaaactttag ttcggaactt cgggctataa aacgaatctg aagaacacag caattttgcg
    17581 ttatctgtta atcgagactg aaatacatga aaaaaaccac attagcactg agtgcactgg
    17641 ctctgagttt aggtttggcg ttatctccgc tctctgcaac ggcggctgag acttcttcag
    17701 caacgacagc ccagcagatg ccaagccttg caccgatgct cgaaaaggtg atgccttcag
    17761 tggtcagcat taacgtagaa ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt
    17821 tccagcagtt cttcggtgat gattctccgt tctgccagga aggttctccg ttccagagct
    17881 ctccgttctg ccaggggggc cagggcggta atggcggcgg ccagcaacag aaattcatgg
    17941 cgctgggttc cggcgtcatc attgatgccg ataaaggcta tgtcgtcacc aacaaccacg
    18001 ttgttgataa cgcgacggtg attaaagtcc agctgagcga tggtcgtaag ttcgacgcga
    18061 agatggttgg caaagatccg cgctctgata tcgcgctgat ccaaatccag aacccgaaaa
    18121 aactgaccgc aattaagatg gcggattctg atgcactgcg cgtgggtgat tacaccgtag
    18181 cgattggtaa cccgtttggt ctgggcgaga cggtaacttc cgggattgtc tctgcgctgg
    18241 ggcgtagcgg cctgaatgct gaaaactacg aaaacttcat ccagaccgat gcagcgatca
    18301 accgtggtaa ctccggtggt gcgctggtta acctgaacgg cgaactgatc ggtatcaaca
    18361 ccgcgatcct cgcaccggac ggcggcaaca tcggtatcgg ttttgctatc ccgagtaaca
    18421 tggtgaaaaa cctgacctcg cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg
    18481 gtattatggg gactgagctg aattccgaac tggcgaaagc gatgaaagtt gacgcccagc
    18541 gcggtgcttt cgtaagccag gttctgccta attcctccgc tgcaaaagcg ggcattaaag
    18601 cgggtgatgt gatcacctca ctgaacggta agccgatcag cagctttgcc gcactgcgtg
    18661 ctcaggtggg tactatgccg gtgggcagca aactgaccct gggcttactg cgcgacggta
    18721 agcaggttaa cgtgaacctg gaactgcagc agagcagcca gaatcaggtt gattccagct
    18781 ccatcttcaa cggcattgaa ggcgctgaga tgagcaacaa aggcaaagat cagggcgtgg
    18841 tagtgaacaa cgtgaagacg ggcactccgg ctgcgcagat cggcctgaag aaaggtgatg
    18901 tgattattgg tgcgaaccag caggcagtga aaaacatcgc tgaactgcgt aaagttctcg
    18961 acagcaaacc gtctgtgctg gcactgaaca ttcagcgcgg cgacagcacc atctacctgt
    19021 taatgcagta atctccctca accccttcct gaatacggga aggggttctc cttacaatct
    19081 gtgaacttcg ccacaactcc atacatcttc atcatccttt gggcatttgc acaatgccgt
    19141 acgttacgta cttccttatg ctaagccgtg cataacggag gacttatggc tggctggcat
    19201 cttgatacca aaatggcgca ggatatcgtg gcacgtacca tgcgcatcat cgataccaat
    19261 atcaacgtaa tggatgcccg cgggcgaatt atcggcagcg gcgatcgtga gcgtattggt
    19321 gaattgcacg aaggtgcatt gctggtactt tcacagggac gagtcgtcga tatcgatgac
    19381 gcggtagcac gtcatctgca cggtgtgcgg caggggatta atctaccgtt acggctggaa
    19441 ggtgaaattg tcggcgtaat tggcctgaca ggtgaaccag agaatctgcg taaatatggc
    19501 gaactggtct gcatgacggc tgaaatgatg ctggaacagt cgcggttgat gcacttgttg
    19561 gcgcaggata gccgtttgcg ggaagaactg gtgatgaacc tgattcaggc agaggagaat
    19621 actcccgcac ttctgaatgg gcgcaacggc tggggatcga tctcaatcaa ccgcgagtgg
    19681 tggctattgt tgaggtcgac agcggtcagc ttggcgtgga cagcgcaatg gcggagttac
    19741 aacaactgca aaacgcgctg actacgcccg agcgtaataa tctggtggcg attgtctcgc
    19801 taaccgaaat ggtggtgttg aaaccggcgt tgaactcttt tgggcgctgg gatgcagaag
    19861 atcatcgtaa gcgagttgaa caactgatta cccgcatgaa agagtacggc cagctgcgtt
    19921 ttcgcgtttc actgggcaac tattttaccg gtcctggcag tattgcccga tcctatcgta
    19981 cggcgaaaac gacgatggtg gtgggtaaac agcggatgcc agaaagtcgc tgctattttt
    20041 atcaggatct gatgttacct gtgttactcg acagtttgcg tggcgactgg caggccaacg
    20101 aactggcgcg accgctggcg cggctgaaag cgatggacaa taacggcttg ctgcgacgaa
    20161 cgctggcggc gtggtttcgc cacaatgtgc aaccgctggc aacgtcaaag gcgttgttta
    20221 ttcatcgtaa taccctggag tatcggctta atcgtatatc ggaactgacc gggcttgatt
    20281 tgggcaattt tgatgacagg ttgctgctgt atgtggcgtt acaactggat gaagagcggt
    20341 agattatgcg ttaaggttga ggcagcgctg gctcattccc ctcagcccga aggggagagg
    20401 ggaatgcccg agttaaccgt caattcggga ttatttacgc gttagttttt ccagatcggc
    20461 ttcaatctcg ctgatcttat tggttacgac tgactccagg tgacgtaagt cgtcgaggat
    20521 cttacgctta agatcaactt cgctgcggtc acgctgacag atttgatcaa gctcatcaat
    20581 gatataccgt agattcgggc tgatttcctg gacttcttta taaccctgac ccacaccatc
    20641 agcgacgacc gttttacgct gacgcggata tttaaactta acgctcttgg cgaaaaactc
    20701 gcctttgtct ttctggaaat agattttcag aatatcgttg ttggcttcct gccggaggct
    20761 gtaacgatca atttcttcag gattggtaat acccagactt ttcagattgt cgtacatagc
    20821 gttacctcaa aatgagtcag taaattgtgc ttattttagc atttggcttg gcccgcccgg
    20881 ctcgactgtg atcgcagaaa gcctttttct tccagacttc tatcatggcg taaaaatcaa
    20941 aaaattacct gctttattgt ggcgatattc catgggatgg attagcacat ttatcttcgt
    21001 tttgcgaatt acatgacagt ccgaaacaag atgaaaaagt atttaaaaaa gggggaggaa
    21061 gaatgagtag gacgaattat tctgagccag aaagcatacg ggcagcatca tgctgcccgt
    21121 atatgtactc ttaatcgatg gtacgcagca gttcgttaat gccgactttg ccgcgagttt
    21181 tcgcgtcaac tttcttaacg ataaccgcac agtagaggct gtatttgcca tcttttgacg
    21241 gcagattacc tgaaacaacc acagaccccg ccggaacgcg accgtagtgg atttcgccgg
    21301 tttcacggtc gtaaatacgg gtgctctgac caatgtatac gcccatagag atgacggaac
    21361 cttcttcgac aatcacccct tcaaccactt cagagcgtgc gccaatgaag cagttatctt
    21421 caataatggt cgggttagcc tgcagcggct ccagcacgcc gccgatgccc acgccaccgg
    21481 aaaggtggac gtttttacca atctgcgcac aagaaccgac ggtcgcccag gtatcaacca
    21541 tggtgccttc atcaacatat gcgccgatgt taacgtaaga cggcatcagc acggtgttac
    21601 gggcaataaa cgcaccctga cgtaccgccg ctggtggcac aacgcggaag ccttctttct
    21661 ggaaacgtgc ttcgtcgtag tcggcgaatt tcatcggcac tttgtcgaag tagcggcttt
    21721 ctgccccttc gatcacctga ttatcattaa tacggaaaga gagcagcacc gcttttttca
    21781 accactgatg cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt gcgccggaat
    21841 ccagcagggc gatcacctga tttaccgctt cgcgggtaac ggtgtctgca ttggctggcg
    21901 tgatctcggc acggcgttca aaagcggttt caataatgtt ctgtaactgc tgcattgtta
    21961 aactcttttc atatcagtaa acacatcacc ctttatcgtt tggattgagg gcctctgtca
    22021 accgctgatg cacttcctgc tgcaactcgt tattaagcgc acgccggtca gcggtggcaa
    22081 ttatgaataa atcttctact cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa
    22141 ttcccagatc ggcaaaaatt ttcccgactc gcgccagcag tccaggttgg tcgagggcga
    22201 tcagttcgag gaacgatttg cggtcggtat gggtcggcaa aaacgttact tcggtttcaa
    22261 cagtaaaatg gcgtaatttg gcgggttggc gacggggctg cggtggctgc cagctacttt
    22321 gcgtcagcac ctgctccaga ccaaaccgaa taacctcatg gcgatctgcg gacagcgggc
    22381 tgccatcggg ttccagcacg ataaaggtat ccatcgccat accgtcgcga gtggtgaaaa
    22441 tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc ggcacagacg gcggcaaaca
    22501 gataggggcg gtccgggctc cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc
    22561 taagcaatac cagcggtttg cttaaatcat gctgtaataa atggcgggca tgccaggcca
    22621 gttgatttgg gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa atttggtgca
    22681 gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag tgcctggagt tggtgatggc
    22741 gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg
    22801 caaaatagag ttcgcgcaac agactttgct tccagctatt ccacagtgtt tcgttggtgg
    22861 cgcaaatgtc agccactgtc aggcatacca gatagcgcag acgattttcc gtttgaactt
    22921 cttcggcaaa ctgcttgatg acttcagggt cctgaatatc gcggcgttgt gctgtcaccg
    22981 acatcagcaa gtgctggcga accagccagg caaccagctg tgtttcgcgt gagttcagcc
    23041 cgtggagttc tgcaaaatgc actacatcct gagcaccgag aatggagtgg tcgccgccgc
    23101 gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt gacggcaggc
    23161 gcggccagac gtccacacac aacggatggc gctggcgcgt ttcttcactg gcaaaactct
    23221 ccagtttcag catcacgcgg atagtatgtt catccaccgt gtaggcgtgg aacagatcaa
    23281 actgcatctg cccgacgata tgcgaccatt gcggcatata cgcgccgagc acgctatggc
    23341 gatgcattgg caatagtccg cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca
    23401 gttttcgtgc ttgcggaata ttacacagcg gttgttgcag atggcgacgg gcatggcgta
    23461 actggcgcag cgtggtggag taaatgccgg tgatcgcact gttgcgcacc atggtgtaga
    23521 acatacgcaa gatggcttcc ggctggcgca taaatagtgt ttcatcacgc aggtcgatta
    23581 gcgtaccgcg tagctgaaac tcatcgtcga gtggacgtgg tttttcgtcg gcgggaaggg
    23641 cgaggatggc ttcatcgaac agttgcagca gcatctggtt gagttcactg acgcggcgtg
    23701 taacgcggaa gtaatccttc atcatccgct cgaccggttc gttgccttca ccactgtaat
    23761 tcagacgctg agcgacgcta agttggcgat cgaataacag acgattatcg taacggctga
    23821 cgaccagatg cagggcaaag cgaatacgcc acagtatatg cagacattcg tttaattccg
    23881 cccgctccgc tgaggttaag aagccaaacc cgaccatttc atccagcgat gttgcgccaa
    23941 aatgacggcg ggccacccat tgcagagtgt gaatatcgcg caagccgcca gggctacttt
    24001 tgatgtctgg ttcaaggttg tagctggtgc catggtaacg ctgatggcgc tggttctgtt
    24061 cttcaacttt cgctgcgtag aacttgtcgg aaggccagaa tccttcgcta aaaatatgtt
    24121 tttgcagttc gaggaagagc gcaacatcgc caattaataa gcgggattcg attaaattgg
    24181 tggcgacggt taaatccgat aacccttcca acatgcactc ttcaagcgtg cgcacactat
    24241 gaccgacttc cagctttaca tcccagagca gcgttaacag ctcgcccact ttttgcgcct
    24301 gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca
    24361 gctcgccacg accgtagcca ccgacggcaa ccaatgccag gtcggcaatc tggctgaatc
    24421 ccgcttcaat ccataatcgt tgcaggagct ggtctatgaa ctcggtacgt gcctcaatca
    24481 actgttctgc agagatccca ttgtcaaagg catcacccag ccaacgctgg aaagtatcga
    24541 tatgggcttt tatcccacag acggttaatt catcacgggg ccaggcgcat ggattttgcg
    24601 gttgaccggg cagggtggag agagcggtgt ttgcgtactg ttctggaagg gtattcattg
    24661 tgcgccaccc ataagattaa attatcgcat taaaaaagcc ggcattcgcc ggcttcatct
    24721 tattcgtcgt gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt caaaatttcg
    24781 cagccgttat cagtcaccac aatagtatgc tcatattgtg cagacaagct gcgatctttg
    24841 gtttttaccg tccagccatc tttcatggtg cggatctctt ttttacccgc gttgaccatt
    24901 ggctcaatgg tgaacgtcat ccccggtttc agcacgacgt tggtttcacg ggagtcatag
    24961 tgcagcacct gcggttcttc atggaagccg cgaccaatac cgtgtccgca atattcacga
    25021 acgacggaga agccttctgc ttcgacaaat ttctgaatcg ccgcaccgat ttcgcgcaga
    25081 ttaatgcctg gttttaccat gcgtagcgcc aggtacaggc tttcttgcgt gatgcggcac
    25141 aggcgttcgc ccatgatggt cggcttaccg acgataaaca ttttcgaggt atcgccgtgg
    25201 aaaccatctt tgattacggt gacatcaatg ttaacgatat cgccatcttt cagcagctta
    25261 gcatcgtccg ggataccgtg gcacaccact tcattaatag agatgcaaac ggatttcgga
    25321 tagccgtgat agccgaggca ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta
    25381 cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa catacggttc gatcatctcc
    25441 agcacttcgg cagccagtcg gccagcgacg cgcatttttt cgatatcttc tggggtcttg
    25501 attgagatag ccattaattc tgtccatcag cgtcggtgat accgacaata tatatgtaag
    25561 tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg
    25621 ccactgaagg ttttctataa tagaaaattc gacgtctgat gctgtacaca gcgccaacaa
    25681 ttattggtgt ccacgacgta tttgtggtat aaagcgcgcc ggacttccga tccatttcgt
    25741 atacacagac tggacggaag cgacaatctc actttgtgta acaacacaca cgtatcggca
    25801 catattccgg ggtgcccttt ggggtcggta atatgggata cgtggaggca taaccccaac
    25861 ttttatatag aggttttaat catggcaact gtttccatgc gcgacatgct caaggctggt
    25921 gttcacttcg gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt catcttcggt
    25981 gcgcgtaaca aagttcacat catcaacctt gagaaaactg taccgatgtt caacgaagct
    26041 ctggctgaac tgaacaagat tgtttctcgc aaaggtaaaa tccttttcgt tggtactaaa
    26101 cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct gcgaccagtt cttcgtgaac
    26161 catcgctggc tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt
    26221 ctgaaagacc tggaaactca gtctcaggac ggtactttcg acaagctgac caagaaagaa
    26281 gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac
    26341 atgggcggtc tgccggacgc tctgtttgta atcgatgctg accacgaaca cattgctatc
    26401 aaagaagcaa acaacttggg tattccggta tttgctatcg ttgataccaa ctctgatccg
    26461 gacggtgttg acttcgttat cccgggtaac gacgacgcaa tccgtgctgt gaccctgtac
    26521 ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc aggatctggc ttcccaggcg
    26581 gaagaaagct tcgtagaagc tgagtaataa ggcttgataa ctcccccaaa atagttcgag
    26641 ttgcagaaag gcggcaagct cgagaattcc cgggagctta catcagtaag tgaccgggat
    26701 gagcgagcga agacaacgca tctgcggcgc gaaatatgaa gggggagagc ccttatagac
    26761 caggtagtac acgtttggtt agggggcctg catatggccc cctttttcac ttttatatct
    26821 gtgtggttta atgccgggca gatcacatct ccgaggattt tagaatggct gaaattaccg
    26881 catccctggt aaaagagctg cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag
    26941 cactgactga agctaacggc gacatcgagc tggcaatcga aaacatgcgt aagtccggtg
    27001 ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa
    27061 tcgacggcaa ctacggcatc attctggaag ttaactgcca gactgacttc gttgcaaaag
    27121 acgctggttt ccaggcgttc gcagacaaag ttctggacgc agctgttgct ggcaaaatca
    27181 ctgacgttga agttctgaaa gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa
    27241 ttggtgaaaa catcaacatt cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt
    27301 atcagcacgg tgcgcgtatc ggcgttctgg ttgctgctaa aggcgctgac gaagagctgg
    27361 ttaaacacat cgctatgcac gttgctgcaa gcaagccaga attcatcaaa ccagaagacg
    27421 tatccgctga agtggtagaa aaagaatacc aggtacagct ggatatcgcg atgcagtctg
    27481 gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg
    27541 aagtttctct gaccggtcag ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc
    27601 tgaaagagca taacgctgaa gtgactggct tcatccgttt cgaagtgggt gaaggcatcg
    27661 agaaagttga gactgacttt gcagcagaag ttgctgcgat gtccaagcag tcttaattat
    27721 caaaaaggag ccgcctgagg gcggcttctt tttgtgccca tcttgtaaat tcagctaacc
    27781 cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta gtattaattc atttcaatcg
    27841 ttgacagtct caggaaagaa acatggctac caatgcaaaa cccgtctata aacgcattct
    27901 gcttaagttg agtggcgaag ctctgcaggg cactgaaggc ttcggtattg atgcaagcat
    27961 actggatcgt atggctcagg aaatcaaaga actggttgaa ctgggtattc aggttggtgt
    28021 ggtgattggt gggggtaacc tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg
    28081 cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg aacggcttgg caatgcgtga
    28141 tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc gctattccat tgaatggcgt
    28201 gtgcgatagc tacagctggg cagaagctat cagcctgttg cgcaacaacc gtgtggtgat
    28261 cctctccgcc ggtacaggta acccgttctt taccaccgac tcagcagctt gcctgcgtgg
    28321 tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa gttgacggcg tgtttaccgc
    28381 tgatccggcg aaagattcaa ccgcaaccat gtacgagcaa ctgacttaca gcgaagtgct
    28441 ggaaaaagag ctgaaagtca tggacctggc ggccttcacg ctggctcgtg accataaatt
    28501 accgattcgt gttttcaata tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga
    28561 aaaagaaggg actttaatca cggaataatt cccgtgatgg ataaataagg gtaagattcc
    28621 gcgtaagtat cgcgggggcg taagtctggt tataaggcgt tattgttgca ggcagtttgg
    28681 tcacggccag cgcgcagcaa ccggagcgta caaaagtacg tgaggatggc gagcactgct
    28741 cggggccaaa atggcaaata aaatagccta ataatccaga cgattacccg taatatgttt
    28801 aatcagggct atacttagca cacttccact gtgtgtgact gtctggtctg actgagacaa
    28861 gttttcaagg attcgtaacg tgattagcga tatcagaaaa gatgctgaag tacgcatgga
    28921 caaatgcgta gaagcgttca aaacccaaat cagcaaaata cgcacgggtc gtgcttctcc
    28981 cagcctgctg gatggcattg tcgtggaata ttacggcacg ccgacgccgc tgcgtcagct
    29041 ggcaagcgta acggtagaag attcccgtac tctgaaaatc aacgtgtttg atcgttcaat
    29101 gtctccggcc gttgaaaaag cgattatggc ctccgatctc ggcctgaacc cgaactctgc
    29161 gggtagcgac atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta aagatctgac
    29221 caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca gtacgtaatg tgcgtcgtga
    29281 cgcgaacgac aaagtgaaag cactgttgaa agataaagag atcagcgaag acgacgatcg
    29341 ccgttctcag gacgatgtac agaaactgac tgatgctgca atcaagaaaa ttgaagcggc
    29401 gctggcagac aaagaagcag aactgatgca gttctgattt cttgaacgac aaaaacgccg
    29461 ctcagtagat ccttgcggat cggctggcgg cgttttgctt tttattctgt ctcaactctg
    29521 gatgtttcat gaagcaactc accattctgg gctcgaccgg ctcgattggt tgcagcacgc
    29581 tggacgtggt gcgccataat cccgaacact tccgcgtagt tgcgctggtg gcaggcaaaa
    29641 atgtcactcg catggtagaa cagtgcctgg aattctctcc ccgctatgcc gtaatggacg
    29701 atgaagcgag tgcgaaactt cttaaaacga tgctacagca acaggggagc cgcaccgaag
    29761 tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct tgaggaggtt gatcaggtga
    29821 tggcagccat tgttggtgct gctgggctgt tacctacgct tgctgcgatc cgcgcgggta
    29881 aaaccatttt gctggccaat aaagaatcac tggttacctg cggacgtctg tttatggacg
    29941 ccgtaaagca gagcaaagcg caattgttac cggtcgatag cgaacataac gctatttttc
    30001 agagtttacc gcaacctatc cagcataatc tgggatacgc tgaccttgag caaaatggcg
    30061 tggtgtccat tttacttacc gggtctggtg gccctttccg tgagacgcca ttgcgcgatt
    30121 tggcaacaat gacgcccgat caagcttgcc gtcatccgaa ctggtcgatg ggacgtaaaa
    30181 tttctgtcga ttcggctacc atgatgaaca aaggtctgga atacattgaa gcgcgttggc
    30241 tgtttaacgc cagcgccagc cagatggaag tgctgattca cccgcagtca gtgattcact
    30301 caatggtgcg ctatcaggac ggcagtgttc tggcgcagct gggggaaccg gatatgcgta
    30361 cgccaattgc ccacaccatg gcatggccga atcgcgtgaa ctctggcgtg aagccgctcg
    30421 atttttgcaa actaagtgcg ttgacatttg ccgcaccgga ttatgagcgt tatccatgcc
    30481 tgaaactggc gatggaggcg ttcgaacaag gccaggcagc gacgacagca ttgaatgccg
    30541 caaacgaaat caccgttgct gcttttcttg cgcaacaaat ccgctttacg gatatcgctg
    30601 cgttgaattt atccgtactg gaaaaaatgg atatgcgcga accacaatgt gtggacgatg
    30661 tgttatctgt tgatgcgaac gcgagtgaag tcgccagaaa agaggtgatg cgtctcgcaa
    30721 gctgaggata atccggctac agagagtcgc gctatttgtt agcgtagggc ttcagtgata
    30781 tagtctgcgc catctgatcg taagtagttg gctttataag gtcaggtatg ccgtggtttt
    30841 acacggcttt tttttgtata ggcttcagta ttcctgagta ccgtaaaccc tgtcagggaa
    30901 taaaaaacgc gtgatgttgt ctgctactca accacttagc gaaaaattgc cagcgcatgg
    30961 ctgccgtcat gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa agcaagggaa
    31021 gattcgtgcc tttgggcata aagccggggc aaaatccgtc cgccgggctg tctcttttgc
    31081 ggccaacaac ggtattgagg cgttaacgct gtatgccttt agtagtgaaa actggaaccg
    31141 accagcgcag gaagtcagtg cgttaatgga actgtttgtg tgggcgctcg atagcgaagt
    31201 aaaaagtctg caccgacata acgtgcgtct gcgtattatt ggcgatacca gtcgttttaa
    31261 ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta acagccggaa ataccggtct
    31321 gacgctgaat attgcggcga actacggtgg acgttgggat atagtccagg gagtcaggca
    31381 actggctgaa aaggtgcagc aaggaaacct gcaaccagat cagatagatg aagagatgtt
    31441 aaaccagcat gtctgtatgc atgaactggc ccctgtagat ttagtaatta ggactggggg
    31501 agagcatcgc attagtaact ttttgctttg gcaaattgcc tatgccgaac tttactttac
    31561 agatgttctc tggcccgatt tcgatgaaca agactttgaa ggggcgttaa atgcctttgc
    31621 taatcgagag cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct gatgggggtc
    31681 gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta atacccgtcg tcatcgcggc
    31741 gttgtttctg ttgccgccgg tggggttcgc cattgtaacg ctggtggtct gcatgctggc
    31801 agcgtgggaa tggggacagc ttagcggttt taccactcgt tcgcagcgag tatggttggc
    31861 ggtgttatgc gggttattgt tggcgctgat gctttttctg ttgccggaat atcaccgaaa
    31921 tattcatcaa ccgctggttg aaatctcact ttgggcttcg ttgggttggt ggattgtcgc
    31981 gctattgctg gtgctgtttt acccaggttc cgcagcaatc tggcgtaact ctaaaacatt
    32041 gcgccttatt tttggcgtgc taaccattgt tcccttcttc tggggcatgc tggcgttacg
    32101 ggcctggcac tatgacgaga atcattacag tggcgcaata tggctgctct atgtcatgat
    32161 cctggtatgg ggcgctgact ccggcgcata tatgtttggc aaattgtttg gtaaacataa
    32221 gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc tttataggtg gactcgctac
    32281 tgcagcggta atctcatggg gttatggcat gtgggcgaat ctcgacgtcg ctcccgtcac
    32341 cttactcatt tgctctattg tcgcagcgtt agcctcagtg ctcggcgatc tgaccgagag
    32401 tatgtttaag cgtgaagcag gaattaagga cagcggtcat ttaattccag gacacggtgg
    32461 tattttagat cgtattgata gcctgacggc tgcggtaccg gtctttgctt gcttgttgtt
    32521 actggtattc aggacgcttt aacggaaggt aatatgctga gttttctctg ggatttggct
    32581 tcgttcatcg ttgcactggg tgtacttatc accgtgcatg aatttggtca tttctgggtt
    32641 gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag ggtttggtaa ggcgctctgg
    32701 cggcgaactg ataagctcgg caccgaatat gttatcgccc tgatcccgtt gggcggttat
    32761 gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg aactccgcca ccatgccttc
    32821 aataataaat ctgtcggcca acgagcggcg attattgccg caggtccggt tgcaaacttc
    32881 atttttgcta tctttgccta ctggctggtt tttattattg gtgtgcctgg cgtacgtccg
    32941 gtggttggcg aaatagcagc caattcgata gctgcggaag cacaaattgc accaggtacg
    33001 gaactaaaag ccgtagatgg tatcgaaacg cctgattggg atgccgtgcg tttgcagttg
    33061 gtcgataaaa ttggcgatga aagcaccacc attacagtag cgccatttgg cagcgaccaa
    33121 cggcgggatg taaagctcga tttacgtcac tgggcgtttg agcctgataa agaagatccg
    33181 gtatcttcgc tggggattcg tcctcgtggg ccgcaaattg aacctgtact ggaaaatgtg
    33241 cagccaaact cggcggcaag caaggcaggt ttgcaagcag gcgacaggat cgttaaagtc
    33301 gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc ttgtccggga taacccgggt
    33361 aaatccttag cgttagaaat cgaaaggcag gggagtccct tgtctttgac attaatcccg
    33421 gggagtaaac cgggtaatgg taaagcgatt ggttttgtcg gtattgagcc gaaagtcatt
    33481 cctttgccag atgagtataa agttgtacgc cagtatgggc cgttcaacgc catcgtcgaa
    33541 gccacggaca aaacgtggca gctgatgaag ctgacggtca gtatgctggg aaaattgatc
    33601 accggtgatg tgaaactgaa caacctcagt gggccgatct ctatcgccaa gggggctggg
    33661 atgacagcgg aactcggggt agtttattac ctgccgtttc ttgcgcttat tagcgtgaac
    33721 ttagggataa ttaacctgtt tccgttgccc gtacttgacg gggggcatct gctgttcctt
    33781 gcgatcgaaa agatcaaggg cggaccggta tccgagcggg ttcaagactt ttgttatcgc
    33841 attggctcga ttctgctggt gctgttaatg gggcttgcac ttttcaatga tttctctcgg
    33901 ttatgagagt tagttaggaa gaacgcataa taacgatggc gatgaaaaag ttgctcatag
    33961 cgtcgctgct gtttagcagc gccaccgtat acggtgctga agggttcgta gtgaaagata
    34021 ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc cctcctcagt atgccggtgc
    34081 gtacaggcga cacggttaat gatgaagata tcagtaatac cattcgcgct ctgtttgcta
    34141 ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga tacccttctg gttcaggtaa
    34201 aagaacgtcc gaccattgcc agcattactt tctccggtaa caaatcggtg aaagatgaca
    34261 tgctgaagca aaacctcgag gcttctggtg tgcgtgtggg cgaatccctc gatcgcacca
    34321 ccattgccga tatcgagaaa ggtctggaag acttctacta cagcgtcggt aaatatagcg
    34381 ccagcgttaa agctgtcgtg accccgctgc cgcgcaaccg tgttgaccta aaactggtgt
    34441 tccaggaagg tgtgtcagct gaaatccagc aaattaacat tgttggtaac catgctttca
    34501 ccaccgacga actgatctct catttccaac tgcgtgacga agtgccgtgg tggaatgtgg
    34561 taggcgatcg taaataccag aaacagaaac tggcgggcga tcttgaaacc ctgcgcagct
    34621 actatctgga ccgcggttat gcccgtttca acatcgactc tacccaggtc agtctgacgc
    34681 cagataaaaa aggtatttac gtcacggtga acatcaccga aggcgatcag tacaagcttt
    34741 ctggcgttga agtgagcggc aaccttgccg ggcactccgc tgaaattgag cagctgacta
    34801 agatcgagcc gggcgagctg tataacggca ctaaagtgac caaaatggaa gatgacatta
    34861 aaaagcttct cggtcgctat ggttatgcct atccgcgcgt acagtcgatg cctgaaatta
    34921 acgatgccga caaaaccgtt aaattacgcg tgaacgttga tgtgggtaac cgtttctacg
    34981 tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga tgccgtcctg cgtcgcgaaa
    35041 tgcgtcagat ggaaggtgca tggttgggga gcgatctggt cgatcagggt aaggagcgtc
    35101 tgaatcgtct gggcttcttt gaaactgtcg ataccgatac ccaacgtgtt ccgggtagcc
    35161 cggaccaggt tgatgtagtc tacaaggtga aagaacgtaa cactggtagc ttcaacttcg
    35221 ggattggtta cggtactgaa agtggcgtga gcttccaggc tggcgtgcaa caggataact
    35281 ggttaggtac aggttatgct gttggtatca acgggaccaa aaacgattac cagacctatg
    35341 ctgaactgtc ggtaaccaac ccgtacttca ccgtagatgg cgtaagcctc ggtggtcgtc
    35401 tcttctataa tgacttccag gcagatgacg ccgacctgtc cgactatacc aacaagagtt
    35461 atggtacaga cgtgacgttg ggcttcccga ttaacgaata taactcgctg cgtgcaggtc
    35521 tgggttatgt acataactcc ctgtccaaca tgcagcctca ggttgcgatg tggcgttatc
    35581 tgtactctat gggtgaacat ccgagcacct ctgatcagga taacagcttc aaaacggacg
    35641 acttcacgtt caactatggt tggacctata acaagcttga ccgtggttac ttcccgacag
    35701 atggttcacg tgtcaacctg accggtaaag tgaccattcc tggatcggat aacgaatact
    35761 acaaagtgac gttagacacg gcgacttatg tgccgatcga tgacgatcac aaatgggttg
    35821 ttctgggtcg tacccgctgg ggttatggtg atggtttagg cggcaaagag atgccgttct
    35881 acgagaactt ctatgccggt ggttccagca ccgtgcgtgg cttccagtcc aataccattg
    35941 gtccgaaagc agtttacttc ccgcatcagg ccagtaatta tgatccggac tatgattacg
    36001 aatgtgcgac tcaggacggc gcgaaagacc tgtgtaaatc ggatgatgct gtaggcggta
    36061 acgccatggc ggttgccagc ctcgagttca tcaccccgac gccgtttatt agcgataagt
    36121 atgctaactc ggttcgtact tcattcttct gggatatggg taccgtttgg gatacaaact
    36181 gggattccag ccaatattct ggatatccgg actatagtga tccaagcaat atccgtatgt
    36241 ctgcgggtat cgcattacaa tggatgtccc cattggggcc gttggtgttc tcctacgccc
    36301 agccgttcaa aaagtacgat ggagacaagg cagaacagtt ccagtttaac atcggtaaaa
    36361 cctggtaagt gttctccaca aaggaatgta gtggtagtgt agcgatgact ttaggcgatc
    36421 aatataagat cgccgggcca cgcaaagaac tgcaccctcc ggtgcaaatg ggatggtaag
    36481 gagtttattg tgaaaaagtg gttattagct gcaggtcttg gtttagcact ggcaacttct
    36541 gctcaggcgg ctgacaaaat tgcaatcgtc aatatgggca gcctgttcca gcaggtagcg
    36601 cagaaaaccg gtgtttctaa cacgctggaa aatgagttca aaggccgtgc cagcgaactg
    36661 cagcgtatgg aaaccgatct gcaggctaaa atgaaaaaac tgcagtccat gaaagcgggc
    36721 agcgatcgca ccaaactgga aaaagacgtg atggctcagc gccagacttt tgctcagaaa
    36781 gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg aagaacgcgg caaactggtt
    36841 actcgtatcc agactgctgt gaaatccgtt gccaacagcc aggatatcga tctggttgtt
    36901 gatgcaaacg ccgttgctta caacagcagc gatgtaaaag acatcactgc cgacgtactg
    36961 aaacaggtta aataagtaat gccttcaatt cgactggctg atttagcgca gcagttggat
    37021 gcagaactac acggtgatgg cgatatcgtc atcaccggcg ttgcgtccat gcaatctgca
    37081 caaacaggtc acattacgtt catggttaac ccaaaatacc gtgagcattt aggcttgtgc
    37141 caggcgtccg cggttgtcat gacccaggac gatcttcctt tcgcgaaaag tgccgcgctg
    37201 gtagtgaaga atccctacct gacttacgcg cgcatggcgc aaattttaga taccacgccg
    37261 cagcccgcgc agaacattgc acccagtgcg gtgatcgacg cgacggcgaa gctgggtaac
    37321 aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg ttgaactggg cgataacgtg
    37381 attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa tcggtgcagg ttcgcgtctc
    37441 tgggcgaacg taaccattta ccatgagatc cagatcggtc agaattgcct gatccagtcc
    37501 ggaacagtgg taggcgcaga cggctttggt tatgccaacg atcgtggtaa ctgggtgaag
    37561 atcccacaga ttggtcgcgt aattattggc gatcgcgtgg agatcggtgc ctgcacaacc
    37621 atcgatcgcg gcgcgctgga tgacactgtt attggcaatg gcgtgatcat tgataaccag
    37681 tgccagattg cacataacgt cgtgattggc gacaatacgg cggttgccgg tggcgtcatt
    37741 atggcgggca gcctgaaaat tggtcgttac tgcatgatcg gcggagccag cgtaatcaac
    37801 gggcatatgg aaatatgcga caaagtgacg gttacgggca tgggtatggt gatgcgtccc
    37861 atcactgaac caggcgtcta ttcctcaggc attccgctgc aacccaacaa agtctggcgc
    37921 aaaaccgctg cactggtgat gaacattgat gacatgagca agcgtctgaa atcgcttgag
    37981 cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt cgccaaactt tacggcctgt
    38041 ctcattctta cgattgcggc aggccgtgtt attattgtcg tttcttatat tttgacagga
    38101 agagtatctt gactactaac actcatactc tgcagattga agagatttta gaacttctgc
    38161 cgcaccgttt cccgttctta ctggtggatc gcgtgctgga ttttgaagaa ggtcgttttc
    38221 tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt ccagggccat ttccctggaa
    38281 aaccgatttt cccgggtgtg ctgattctgg aagcaatggc acaggcaaca ggtattctgg
    38341 cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta ctacttcgct ggtattgacg
    38401 aagcgcgctt caagcgcccg gtcgtgcctg gcgatcaaat gatcatggaa gtcactttcg
    38461 aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc tctggtcgat ggtaaagtag
    38521 tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc ctgatacgtg attgataaat
    38581 ccgcctttgt gcatccaacc gccattgtgg aagagggcgc gtcaattggc gcgaacgcac
    38641 acattggtcc tttttgtatc gttggacccc atgtcgaaat tggtgagggt accgtactga
    38701 aatctcacgt tgtcgtgaat ggtcatacta aaattggccg cgataatgag atttatcagt
    38761 ttgcctccat cggcgaagtt aaccaggatt tgaaatatgc tggcgaaccg acccgtgtgg
    38821 aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat tcatcgtggc acagtccagg
    38881 gcggtggatt gacgaaggtg ggcagcgaca acttactgat gatcaacgcg cacattgcgc
    38941 acgattgtac ggtaggtaac cgctgtattc tcgccaacaa cgcaacgctg gcgggtcacg
    39001 tatcggttga cgacttcgcg atcatcggcg gcatgaccgc agtccatcag ttctgcatca
    39061 ttggtgcgca tgtgatggtt ggcggctgct ccggtgtggc gcaggacgtc cctccttatg
    39121 tcattgcgca gggtaaccac gcaacgccgt tcggtgtcaa tatcgaaggg ctgaagcgcc
    39181 gcggattcag ccgtgaggcg attaccgcta tccgcaatgc gtataagcta atttatcgta
    39241 gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga actggcggaa acatatccgg
    39301 aagtgaaagc ctttaccgat ttctttgcac gctcaacgcg cggtctgatt cgttaatgac
    39361 tgaccagcgt ccattaacga ttgccctggt cgccggagaa acctccggcg atatcctggg
    39421 ggccggttta atccgcgctc tgaaagaacg tgtgcccaac gcccgctttg ttggtgttgc
    39481 cgggccacga atgcaggctg aaggctgcga agcctggtac gaaatggaag aactggcggt
    39541 gatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta ctgcatattc gtgccgatct
    39601 gacaaagcgt tttggcgaac tgaagccaga tgtttttgtt ggtattgatg cgcccgactt
    39661 caatattact cttgaaggta acctcaaaaa gcagggtatc aaaaccattc attacgtcag
    39721 tccatccgtc tgggcgtggc gacagaaacg cgttttcaaa ataggcagag ccaccgatct
    39781 ggtgctcgca tttctgcctt tcgaaaaagc gttttatgac aaatacaatg taccgtgccg
    39841 ctttatcggt cataccatgg ctgatgccat gccgttagat ccagataaaa atggtgcccg
    39901 tgatgtgctg gggatccctt acgatgccca ctgtctggca ttgttgccgg gcagccgtgg
    39961 tgcagaagtc gaaatgctta gtgccgattt cttgaaaact gcccagcttt tgcgccagac
    40021 atatccggat ctcgaaatcg tggtgccgct ggtgaatgcc aaacgccgcg agcagtttga
    40081 acgcatcaaa gctgaagtcg cgccagacct gtcggttcat ttgctggatg gaatgggccg
    40141 tgaggcgatg gtcgccagcg atgcggcact actggcgtcg gggacggcag ccctggagtg
    40201 tatgctggcg aaatgcccga tggtggtggg atatcgcatg aagcctttta ccttctggtt
    40261 ggcgaagcgg ctggtgaaaa ctgattatgt ctcgctgcca aatctgctgg cgggcagaga
    40321 gttagtcaaa gagttattgc aggaagagtg tgagccgcaa aaactggctg cggcgctgtt
    40381 accgctgttg gcgaacggga aaaccagcca cgcgatgcac gataccttcc gtgaactgca
    40441 tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa gccgttctgg agttagcaca
    40501 atgatcgaat ttgtttatcc gcacacgcag ctggttgcgg gtgtggatga agtcggacgc
    40561 gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc ttgacccggc gcgcccgatt
    40621 gccgggctga atgattccaa aaagctgagc gaaaaacgcc gtctggcgct ctatgaagag
    40681 atcaaagaga aagcgttgag ctggagtctg ggccgcgcgg aaccccacga aatcgacgag
    40741 ctgaacattc ttcatgcgac catgctggcg atgcagcgtg ccgtcgctgg gctgcatatt
    40801 gcgccggaat atgtgttgat tgatggtaac cgctgcccga aattaccgat gcctgcgatg
    40861 gctgtggtga aaggggatag ccgcgtacca gaaatcagtg ccgcgtctat cctggcgaaa
    40921 gtgacgcgtg acgccgaaat ggcggcgctg gatattgttt tcccgcaata tggttttgcc
    40981 caacataaag ggtatccaac cgcttttcat ctggaaaaac tggctgaata tggcgcgact
    41041 gaacatcatc gtcgcagctt tgggcctgtc aaacgcgcac tgggacttgc gtcctgattc
    41101 ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg aaccacgttt cgtacacctg
    41161 cgggtgcaca gcgactactc gatgatcgat ggcttggcca aaaccgcacc gctggtaaaa
    41221 aaggcggcgg cgttgggtat gccagcactg gcgatcaccg atttcaccaa cctttgcggt
    41281 ctggtgaagt tctacggagc gggacatggc gcagggatta agcctatcgt cggggcagat
    41341 tttaatgtcc agtgcgacct gctgggtgat gagttaaccc acctgacggt actggcggcg
    41401 aacaataccg gctatcagaa tctggcgttg ctgatctcaa aagcgtatca gcgcgggtac
    41461 ggtgccgccg ggccgatcat cgatcgcgac tggcttatcg aattaaacga agggttgatc
    41521 cttctttccg gcgggcgcat gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg
    41581 ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta ttttctcgag
    41641 ctgatccgca ccggcaggcc ggatgaagaa agctatctgc acgcggcggt ggaactggcg
    41701 gaagcgcgcg gtttgcccgt cgtggcgact aacgatgtgc gctttatcga cagcagcgac
    41761 tttgacgcac acgaaatccg cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa
    41821 cgcccgcgta actattcgcc gcagcaatat atgcgtagcg aagaggagat gtgtgagctg
    41881 tttgccgaca tcccagaagc ccttgccaac accgttgaga tcgccaaacg ctgtaacgta
    41941 actatgcgtc ttggtgaata cttcctgccg cagttcccga ccggggacat gagcaccgaa
    42001 gattatctgg tcaagcgtgc aaaagaaggc ctggaagagc gtctggcatt tttattccct
    42061 gacgaggaag aacgtcttaa acgccgcccg gaatatgacg aacgtctgga gactgaactt
    42121 caggttatca accagatggg cttcccgggc tacttcctca tcgttatgga atttatccag
    42181 tggtcgaaag ataacggcgt accggtaggg ccaggccgtg gctccggtgc gggttcactg
    42241 gtggcctacg cgctgaaaat caccgacctc gatccgctgg aatttgacct gctgttcgaa
    42301 cgtttcctta acccggaacg tgtctccatg cctgacttcg acgttgactt ctgtatggag
    42361 aaacgcgatc aggttatcga acacgtggcg gacatgtacg gtcgtgatgc ggtatcgcag
    42421 attatcacct tcggtacgat ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg
    42481 gggcatccgt acggctttgt cgaccgtatc tcgaaactga tcccgcccga tccggggatg
    42541 acgctggcga aagcgtttga agccgagccg cagctgccgg aaatctacga agcggatgaa
    42601 gaagttaagg cgctgatcga catggcgcgc aaactggaag gggtcacccg taacgccggt
    42661 aagcacgccg gtggggtggt tatcgcgccg accaaaatta ccgattttgc gccgctttat
    42721 tgcgatgaag agggcaaaca tccggtcacc cagtttgata aaagcgacgt tgaatacgcc
    42781 ggactggtga agttcgactt ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg
    42841 gagatgatca acaagcggcg ggcgaagaat ggcgagccac cgctggatat cgccgcgatc
    42901 ccgctggatg acaagaaaag cttcgacatg ctgcaacgct cggaaaccac ggcggtattc
    42961 cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa
    43021 gatatgatcg cactggtggc actgttccgc cctggtccgt tgcagtcagg gatggtggat
    43081 aactttatcg accgtaaaca tggtcgtgaa gagatctcct atccggacgt gcagtggcag
    43141 catgaaagcc tgaaatcggt actggagcca acctacggca ttatcttgta tcaggaacag
    43201 gtcatgcaga ttgcccaggt gctttctggt tataccctcg gtggcgcgga tatgctgcgc
    43261 cgtgcgatgg gtaagaaaaa gccggaagag atggctaagc agcgttctgt atttgctgaa
    43321 ggtgcagaaa agaacggaat caacgccgaa ctggcgatga aaatcttcga cctggtggag
    43381 aaattcgcgg gttacggatt taacaaatcg cactctgcgg cctatgcttt ggtgtcatat
    43441 caaacgttat ggctgaaagc gcactatccg gcggagttta tggcggcggt aatgaccgcc
    43501 gatatggaca acaccgagaa ggtggtgggc ctggtggatg agtgctggcg gatggggctg
    43561 aaaatcctgc caccagatat aaactccggt ctttaccatt tccacgtcaa cgacgacggc
    43621 gaaatcgtgt atggtattgg cgccatcaaa ggggtaggtg aaggtccgat tgaggccatc
    43681 atcgaagccc gtaataaagg cggctacttc cgcgaactgt ttgatctctg cgcccgtacc
    43741 gacaccaaaa agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac
    43801 cgtcttgggc cacaccgcgc ggcgctgatg aactcgctgg gtgatgcgtt aaaagcggca
    43861 gatcaacacg cgaaagcgga agctatcggt caggccgata tgttcggcgt gctggccgaa
    43921 gagccggaac aaattgaaca atcctacgcc agctgccaac cgtggccgga gcaggtggta
    43981 ttagatgggg aacgtgaaac gttaggcctg tacctgaccg gacaccctat caaccagtat
    44041 ttaaaagaga ttgagcgtta tgtcggaggc gtaaggctga aagacatgca cccgacagaa
    44101 cgtggtaaag tcatcacggc tgcggggctc gttgttgccg cgcgggttat ggtcaccaag
    44161 cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg
    44221 ttgtttactg acgccctgga taaataccag caattgctgg aaaaagaccg catacttatc
    44281 gtcagcggac aggtcagctt tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa
    44341 gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg
    44401 gacaggcaaa ttgatgacca gcttttaaac cgactccgtc agtctctgga accccaccgc
    44461 tctgggacaa ttccagtaca tctctactat cagagggcgg atgcacgcgc gcggttgcgt
    44521 tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat taaacgatct ccgtggcctc
    44581 attggttcgg agcaggtgga actggagttt gactaataca ggaatactat gagtctgaat
    44641 ttccttgatt ttgaacagcc gattgcagag ctggaagcga aaatcgattc tctgactgcg
    44701 gttagccgtc aggatgagaa actggatatt aacatcgatg aagaagtgca tcgtctgcgt
    44761 gaaaaaagcg tagaactgac gcgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg
    44821 caactggcac gccatccgca gcgtccttat accctggatt acgttcgcct ggcatttgat
    44881 gaatttgacg aactggctgg cgaccgtgcg tatgcagacg ataaagctat cgtcggtggt
    44941 atcgcccgtc tcgatggtcg tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc
    45001 aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg caaagcactg
    45061 cgtctgatgc aaatggctga acgctttaag atgcctatca tcacctttat cgacaccccg
    45121 ggggcttatc ctggcgtggg cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac
    45181 ctgcgtgaaa tgtctcgcct tggcgtaccg gtagtttgta cggttatcgg tgaaggtggt
    45241 tctggcggtg cgctggcgat tggcgtgggc gataaagtga atatgctgca atacagcacc
    45301 tattccgtta tctcgccgga aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg
    45361 ccgctggcgg ctgaagcgat gggtatcatc gctccgcgtc tgaaagagct gaaactgatc
    45421 gactccatca tcccggaacc gctgggcggc gctcaccgta acccggaagc gatggcggca
    45481 tcgttgaaag cgcaactgct ggcggatctg gccgatctcg atgtgttaag cactgaagat
    45541 ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt
    45601 ctgaaaaagg gtcacttcgg tggccctttt ttatcgccac ggtttgagca ggctatgatt
    45661 aaggaaggat tttccaggag gaacacatga acatcattgc cattatggga ccgcatggcg
    45721 tcttttataa agatgagccc atcaaagaac tggagtcggc gctggtggcg caaggctttc
    45781 agattatctg gccacaaaac agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa
    45841 tttgcggcgt gatttttgac tgggatgagt acagtctcga tttatgcagc gatatcaatc
    45901 aacttaatga atatctcccg ctttatgcct tcatcaacac ccactcgacg atggatgtca
    45961 gcgtgcagga tatgcggatg gcgctctggt tttttgaata tgcgctgggg caggcggaag
    46021 atatcgccat tcgtatgcgt cagtacacca acgaatatct tgataacatt acgccgccgt
    46081 tcacgaaagc cttgtttacc tacgtcaaag agcggaagta caccttttgt acgccggggc
    46141 atatgggcgg caccgcatat caaaaaagcc cggttggctg tctgttttat gattttttcg
    46201 gcgggaatac ccttaaggct gatgtctcta tttcggtcac cgagcttggt tcgttgcttg
    46261 accacaccgg gccacatctg gaagcggaag agtacatcgc gcggactttt ggcgcggaac
    46321 agagttatat cgttaccaac ggaacatcga cgtcgaacaa aattgtgggt atgtacgccg
    46381 cgccatccgg cagtacgctg ttgatcgacc gcaactgcca taaatcactg gcgcatctgt
    46441 tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc
    46501 tgggggggat cccgcgccgt gaatttactc gcgacagcat cgaagagaaa gtcgctgcca
    46561 ctacgcaagc acaatggccg gttcatgcgg tgatcaccaa ctccacctat gatggtttgc
    46621 tctacaacac cgactggatc aaacagacgc tggatgtccc gtcgatccac ttcgattccg
    46681 cctgggtgcc gtatactcat tttcatccaa tctaccaggg taaaagtggt atgagcggcg
    46741 agcgtgttgc gggaaaagtt ttcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt
    46801 tatcgcaggc ttcactgatc cacattaaag gcgagtatga cgaagaggcg tttaacgaag
    46861 cctttatgat gcataccacc acctcgccca gttatcccat tgttgcttcg gttgagacgg
    46921 cggcggcgat gctgcgtggt aatccgggca aacggctgat taaccgttcg gtggagcggg
    46981 cgctgcattt tcgcaaagag gtccagcggc tgcgggaaga gtctgacggt tggtttttcg
    47041 atatctggca accgccgcag gtggatgaag ccgaatgcag gcccgttgcg cctggcgaac
    47101 aatggcacgg ctttaacgat gcggatgccg atcatatgtt tctcgatccg gttaaagtca
    47161 ctattttgac accggggatg gacgaacagg gcaatatgag tgaggagggg atcccggcgg
    47221 cgctggtagc aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc ggcccttata
    47281 acctgctgtt tctctttagt attggcatcg ataaaaccaa agcaatggga ttattgcgtg
    47341 ggttgacgga atttaagcgc tcttacgatc tcaacctgcg gatcaaaaat atgctgcccg
    47401 atctctatgc tgaagatccc gatttctacc acaatatgcg tattcaggat ctggcgcaag
    47461 ggatccataa gctgattcgt aaacacgatc tttccggttt gatgttgcgg gcattcgata
    47521 ctttgccgga aatgatcatg acgccacatc aggcatggca gagacagatt aaaggtgaag
    47581 tagaaaccat tgcgctggaa caactggtcg gtagagtatc ggcaaatatg atcctgcctt
    47641 atccaccggg cgtaccgctg ttgatgccag gagaaatgct gaccaaagag agccgcacgg
    47701 tactcgattt tctactgatg ctttgttccg tagggcaaca ttaccccggt tttgaaacgg
    47761 atattcacgg cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg
    47821 cgggataact tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga
    47881 cgttatgctg ggtttaaaac aggttcacca tattgcgatt attgcgacgg attatgcggt
    47941 gagcaaagct ttctactgcg atattcttgg tttcacgctg caaagcgaag tctatcgcga
    48001 agcgcgcgac tcgtggaaag gggatttggc gcttaatggg caatatgtga ttgagctttt
    48061 ctcttttccg ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct
    48121 ggcttttagc gttgatgaca tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa
    48181 gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc ttcaccttct ttaacgatcc
    48241 ggacgggctg ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg
    48301 taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat gacactcacg ctcaatagac
    48361 aacttctcac ctcacgccag attctggtgg cctttagcgg cgggcttgac tccaccgttc
    48421 tgctgcatca gttggtgcag tggcggacgg aaaatccggg cgtcactctg cgcgctattc
    48481 atgtgcatca cggtttaagt gccaatgccg atgcctgggt tacgcattgc gaaaacgtct
    48541 gccaacagtg gcaggtgccg ctggtggtcg aacgcgtaca acttgcgcaa gaggggctgg
    48601 ggattgaggc ccaggcgcgg caggcacgtt atcaggcatt tgcccgtacc ctattgcccg
    48661 gcgaagtgct ggtcaccgcg caacatctcg acgatcaatg tgaaaccttt ctgctggcgc
    48721 taaaacgcgg tagcggccct gccgggcttt cggctatggc ggaagtttcg gagtttgctg
    48781 gaacacggct tatccgcccg ttgcttgccc gcacgcgggg ggaactggag cagtgggcat
    48841 tggcgcatgg tttacgctgg attgaagacg aaagtaatca ggacgactca tacgatcgta
    48901 actttctgcg cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat tttgcggaag
    48961 caacggcccg cagcgccaca ctttgtgctg aacaagagag tctgttggat gaactgctgg
    49021 cggatgattt agcacactgc caaacgtcgc aggggacgct gcagattgcg ccaatgctgg
    49081 cgatgagtga tgcccgtcgc gcggcgatta ttcgccgctg gctggcaggg cagaatgcac
    49141 cgatgccttc ccgcgacgcg ctggtgcgca tctggcagga agtggcgctg gcgcgggaag
    49201 atgcttcccc ctgtttacgt ttgggcgcct ttgaaatccg ccgctatcag tcgcaactgt
    49261 ggtggattaa atccgtcacc gggcaaagcg aaaccattgt gctgtggcag acgtggctac
    49321 aaccgctgga attaccggcg gggctgggaa ccgtacagct tactgcggga ggcgatattc
    49381 gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata
    49441 ttgtcgggcg caacggcgga cgtaagctga agaaaatctg gcaagagtta ggcgtgccgc
    49501 cgtggctacg tgacaccacg ccactgctgt tttatggaga aacgctgatt gcggcggcag
    49561 gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa tggcgtaagt tttgtctggc
    49621 agaaaacgct tagttaagtg aaagccggat aagacgcatc aaacgtcgca tccggcgaaa
    49681 gtcaatcagg attcgcttac caccaccgta ccgatttcag ggtggctaaa gctggtaatt
    49741 ttatccagac gcagctcgcg ggtttcgcca gcggcctcga cgaccagata ctccacattt
    49801 ttgcgggaga ctaaatcact ggcttttgcc tgcaattttt cgccatcttt cagctcaagt
    49861 gtcagcatta aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattaatt
    49921 ggttgatacg tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc
    49981 tgctttttcc ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt
    50041 caaaacgcag cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta
    50101 ccgcttgcgt attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta
    50161 accgtggatg caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca
    50221 cactcatttc tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga
    50281 ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg
    50341 gccgatctcg accgcaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca
    50401 gaaacccagg agcgtatgat gctgcgcttg ctggcgtggc tgaaatatgc tgatgaacgt
    50461 ctgcaattta cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat
    50521 cacctgggca ttgatttgtg gattgagctg gggctgccgg atgagcgacg aattaagaaa
    50581 gcctgcaccc aggccgcaga agtggcgctg tttacctata atagtcgggc ggcgcaaatc
    50641 tggtggcagc aaaatcagag caaatgtgtg cagtttgcca atctttccgt ctggtatctg
    50701 gacgatgaac aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca
    50761 acgattcagg atggtgttat ctggttatcg gatgataaga ataatctgga agttaactta
    50821 accgcctggc aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg
    50881 agcttgagat caccgccatt cgtgcgcagg gcgctggcgg gcagcatgtt aataagacct
    50941 caacggctat tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag
    51001 agcgtctgct cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg
    51061 cacaggaata ccgcagtcag gaactgaacc gcgaagccgc tctggcccgg ctggtggcag
    51121 tgattaaaga tttaacaaaa gaacaaaaag cccgacgacc cacgcggccc acccgtgcat
    51181 cgaaagagcg caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca
    51241 aagtacgcag cggtcgggaa taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca
    51301 gcgatggctg taatcagcct ctttactctg atgggatgta ataatcgggc cgaagtcgat
    51361 acgctttctc cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg
    51421 ctaccgtgtg ccgattgcga aggaatcgaa atctctctgt tcctcgaaaa agacggaaca
    51481 tgggtgatga atgagcgtta tctcggtgct cgtgaagaac cttcctcctt cgcttcctac
    51541 ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca
    51601 tattatcggg cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa
    51661 tcgcagttca actatacgct ggaaccggca caatccagtt tacctatgac gccgatgacc
    51721 ctgcgaggca tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga
    51781 aaacgtttca tggtagcgaa taacgcagag ctggagcgta gctacctggc tgcgcgcggt
    51841 cacagtgaaa aaccggtgtt actgtcagta gaaggtcact ttacgcttga ggctaatccg
    51901 gatactggtg cgccaaccaa agtattagcg cccgatacgg cgggtaaatt ttacccgaat
    51961 aaggattgca gtagtttggg gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc
    52021 ctgatgcgct gcgcttatca ggcctacagc atataatgca acattttgaa ttagcacgat
    52081 tttgtaggcc ggataaggcg tttacgccgc atccggcatt ttgtgcacga ggctggcttt
    52141 atcactgagt cagcccaaca aagcgacccg ccttgatata aatcccaggc ttcaggtgcc
    52201 caacatactg taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca
    52261 tcagatcggc agggctaatc cagcccgatt gcgccagtgt aagtggatgc ccggatttgg
    52321 caaatgcatc agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcacacac
    52381 tgcttaactg cgctttcgcc aggtcgctga cgcactgttg gcgaaaatcc tcggagaacg
    52441 gattcagtga gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat
    52501 agttataacc gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg
    52561 gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa
    52621 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga
    52681 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa
    52741 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg
    52801 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat
    52861 ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa
    52921 gaaagctggg gagaagcagg cggcagtacg cctttggttt atccatttta tacaatccat
    52981 gtaaaaaagg gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc
    53041 agatattcga cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta
    53101 tattcgatat cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg
    53161 atcagttcca tatcagcaaa catcacgccc ggacgctctt tgcggtcatc cagcagcact
    53221 tcgatacctt gtgcacgcag ttcgctgtac agtttctcag caagctcttg tacgcggaag
    53281 gatttgtgca tgttcatcgg cagaatcgcc acctggaacg gcgcgatagc gtcaggccat
    53341 acgatgccgc gttcgtcgta gttctgctca atcgccgcag ctaccacacg cgttaccccg
    53401 ataccgtagc aacccatcgt caggatttgg ttacggccat cttcaccctg tacggaggct
    53461 ttcagtgctt cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt
    53521 ttgatcagca gcgtaccctg gccatccggg cttggatcgc cagccaccac gttacggata
    53581 tctgcaactt ccggggtagc gacatcgcga tcccagttga taccgaagta gtgtttacca
    53641 tcgatgttag caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccacc
    53701 ggaatcggca tgtttaccgg acccagtgaa cccggaccgg ctttaaccac agcacgaatt
    53761 tcttcttcgg tcgcgaaagt cagcgggctt gcaacctgcg gcagtttttc tgctttaact
    53821 tcgttcagct cgtgatcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca
    53881 accgctttaa ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc
    53941 agttccgcga tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca
    54001 gcgcgcggtt ctttcggcgc gatagcttct gccagttcaa tgttcgctgc atagtcagag
    54061 gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag
    54121 gcgctgccgc cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg
    54181 ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga
    54241 gaagtatgga aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa
    54301 cgcggacgca cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt
    54361 ttgtaagagc taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg
    54421 agtacgaacg gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc
    54481 caacgaccac tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatc
    54541 gcaccggcgt tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg
    54601 ccggtcggca gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc
    54661 atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg
    54721 tattggctag tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga
    54781 gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg
    54841 cgtgaattta acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc
    54901 gaacgttaaa atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat
    54961 aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac
    55021 gcttctccag cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg
    55081 cagctggcgc actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga
    55141 gatacggttt gatatccact accggcgtac catcgaccag atccagactg ccgagcttca
    55201 gaatcacgct gcctttatgg caaacaacct ctttcagctc taccagcgac atgccaattg
    55261 ggttagggcg gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc
    55321 gcggtggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga
    55381 tccataaatg gctgaatgct tccaggccgc gaacggcgtc ggcctggttg taggaagcaa
    55441 tgagatgcag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt
    55501 tttctttata gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc
    55561 cgtaatgtta agcgcagagc cgatacatac agcctgacga tagcagcccg gcgtaccgct
    55621 ggtgacttcg cagctatgca gtaatacagc attggctttc attttagagg cattgatttg
    55681 catctgctta cgtgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga
    55741 gtcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt
    55801 ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg
    55861 gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag
    55921 catgagtgct actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa
    55981 aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga
    56041 atgatattac aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt
    56101 aaacactttg tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt
    56161 cacgttctcg gcgtctttgt tatcttcacg cgttacgatc aggtttacgt atggggagtc
    56221 tttatcttcg acaaagatac cgtctttcgc cggagtcagg ccaatctggc tggcataggt
    56281 ggtattgata actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc
    56341 ttccagttca acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag
    56401 caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg
    56461 accaagatta gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag
    56521 tgatttgatt ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac
    56581 cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt
    56641 ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac
    56701 cagctcaacg tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg
    56761 ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc
    56821 gcagcctacc agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt
    56881 gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt
    56941 gacagcccgg acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa
    57001 taccagtacc gtattcatca ccgtcgcgtt gtagccgatg tagccatact gatagccaat
    57061 ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat
    57121 cagggtgata gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttaca
    57181 gacgatctgc attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat
    57241 ctccagcaga gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg
    57301 aacaattgct gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat
    57361 ccataccagc aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt
    57421 acgatacagc ttcgcgttag caataatttg ccccggacgc gtgacataaa gcagaacgcc
    57481 aaccggcaga ccaatcacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc
    57541 ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt
    57601 ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca
    57661 tttcagtcag catgatgccg aatttaacgc caccggcgta gtccatctgc gcgctaataa
    57721 tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt
    57781 gaccggtaaa ctccagacgc agcatcggca cgcagtcagt aaatggctcc gtttgcagac
    57841 gttcctggta atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca
    57901 gcggcgtttt cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat
    57961 tgctgatgac cgccacgcaa tcacaaatac gcttcacaac gtccatttcg tgggtgatca
    58021 acagaatcgt caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg
    58081 tcgttgccgg gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg
    58141 ctaatgcacg ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc
    58201 tatcatgctt atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct
    58261 cgtctttcgg tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg
    58321 aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact
    58381 cggattctga cagcgtggtc aattcctggc catcgaccag cacgctaccc tcggttgggc
    58441 gctccagcag gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa
    58501 cgccataaat ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc
    58561 gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat
    58621 cgtcattaag ttagtcgtag catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat
    58681 ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc
    58741 aggtcgaaac atgcgatact agcgtcccat gccttattaa ggagctataa aaggtggcga
    58801 agagcgtacc cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg
    58861 tccatgagat cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa
    58921 aaaaaatggg ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat
    58981 ttaccgaagc acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag
    59041 atgtcgatct ggatggtatc tattattgcc cgcatcatcc gcaggggagt gttgaagagt
    59101 ttcgccaggt ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt
    59161 atttgcatat tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg
    59221 cagcggttgc ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac
    59281 ctgaagcaga aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga
    59341 taaaaaagca gcaaaaaccg gcgcaatgat taaaagatga acggttgaaa taaaaatgca
    59401 tttttccgct tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcggat
    59461 gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaaatcctg aaattcaggg
    59521 ttgactctga aagaggaaag tgtgatatac gccacctcgc gacagtgagc tgaaagctgc
    59581 gtcgcaactg ctctttaaca atttatcaga caatctgtgt gggcactcga agatacggat
    59641 tcttaacgtc gcaagacgat aaatgaatac caagtctcaa gagtgaacac gtaattcatt
    59701 acgaagttta attctttgag catcaaactt ttaaattgaa gagtttgatc atggctcaga
    59761 ttgaacgctg gcggcaggcc taacacatgc aagtc
//
LOCUS       DAJTUN010000007        57508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTUN010000007 DAJTUN010000000
VERSION     DAJTUN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.007"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            640..1068
                     /gene="uspC"
                     /locus_tag="OO470_000410"
     CDS             640..1068
                     /gene="uspC"
                     /locus_tag="OO470_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6249786.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1075..2489)
                     /gene="otsA"
                     /locus_tag="OO470_000411"
                     /pseudo
     CDS             complement(1075..2489)
                     /gene="otsA"
                     /locus_tag="OO470_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2464..3264)
                     /gene="otsB"
                     /locus_tag="OO470_000412"
     CDS             complement(2464..3264)
                     /gene="otsB"
                     /locus_tag="OO470_000412"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6249787.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3431..4417)
                     /gene="araH"
                     /locus_tag="OO470_000413"
     CDS             complement(3431..4417)
                     /gene="araH"
                     /locus_tag="OO470_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6249788.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4432..5946)
                     /gene="araG"
                     /locus_tag="OO470_000414"
     CDS             complement(4432..5946)
                     /gene="araG"
                     /locus_tag="OO470_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6249789.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6016..7005)
                     /gene="araF"
                     /locus_tag="OO470_000415"
                     /pseudo
     CDS             complement(6016..7005)
                     /gene="araF"
                     /locus_tag="OO470_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7802..8305
                     /locus_tag="OO470_000416"
     CDS             7802..8305
                     /locus_tag="OO470_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6249790.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8385..8636)
                     /locus_tag="OO470_000417"
     CDS             complement(8385..8636)
                     /locus_tag="OO470_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6249791.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8751..8837)
                     /gene="azuC"
                     /locus_tag="OO470_000418"
     CDS             complement(8751..8837)
                     /gene="azuC"
                     /locus_tag="OO470_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6249792.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9100..9423
                     /locus_tag="OO470_000419"
     CDS             9100..9423
                     /locus_tag="OO470_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6249793.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9594..10091
                     /gene="ftnA"
                     /locus_tag="OO470_000420"
     CDS             9594..10091
                     /gene="ftnA"
                     /locus_tag="OO470_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6249794.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10128..10367)
                     /locus_tag="OO470_000421"
     CDS             complement(10128..10367)
                     /locus_tag="OO470_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6249795.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10557..11768
                     /gene="tyrP"
                     /locus_tag="OO470_000422"
     CDS             10557..11768
                     /gene="tyrP"
                     /locus_tag="OO470_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6249796.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11830..12495)
                     /gene="yecA"
                     /locus_tag="OO470_000423"
     CDS             complement(11830..12495)
                     /gene="yecA"
                     /locus_tag="OO470_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6249797.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12691..12777)
                     /locus_tag="OO470_000424"
     tRNA            complement(12691..12777)
                     /locus_tag="OO470_000424"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12741..12743),aa:Leu,seq:taa)
     gene            complement(12790..12863)
                     /locus_tag="OO470_000425"
     tRNA            complement(12790..12863)
                     /locus_tag="OO470_000425"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12829..12831),aa:Cys,seq:gca)
     gene            complement(12918..12993)
                     /locus_tag="OO470_000426"
     tRNA            complement(12918..12993)
                     /locus_tag="OO470_000426"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12958..12960),aa:Gly,seq:gcc)
     gene            complement(13145..13693)
                     /gene="pgsA"
                     /locus_tag="OO470_000427"
     CDS             complement(13145..13693)
                     /gene="pgsA"
                     /locus_tag="OO470_000427"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6249798.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13750..15582)
                     /gene="uvrC"
                     /locus_tag="OO470_000428"
     CDS             complement(13750..15582)
                     /gene="uvrC"
                     /locus_tag="OO470_000428"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6249799.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15579..16235)
                     /gene="uvrY"
                     /locus_tag="OO470_000429"
     CDS             complement(15579..16235)
                     /gene="uvrY"
                     /locus_tag="OO470_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6249800.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16531..16707
                     /locus_tag="OO470_000430"
     CDS             16531..16707
                     /locus_tag="OO470_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249801.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16694..16918
                     /locus_tag="OO470_000431"
     CDS             16694..16918
                     /locus_tag="OO470_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6249802.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16951..17571)
                     /gene="sdiA"
                     /locus_tag="OO470_000432"
                     /pseudo
     CDS             complement(16951..17571)
                     /gene="sdiA"
                     /locus_tag="OO470_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17801..18553)
                     /gene="yecC"
                     /locus_tag="OO470_000433"
     CDS             complement(17801..18553)
                     /gene="yecC"
                     /locus_tag="OO470_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6249803.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18550..19218)
                     /gene="tcyL"
                     /locus_tag="OO470_000434"
     CDS             complement(18550..19218)
                     /gene="tcyL"
                     /locus_tag="OO470_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6249804.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19233..20219)
                     /gene="dcyD"
                     /locus_tag="OO470_000435"
     CDS             complement(19233..20219)
                     /gene="dcyD"
                     /locus_tag="OO470_000435"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6249805.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20324..21124)
                     /gene="tcyJ"
                     /locus_tag="OO470_000436"
     CDS             complement(20324..21124)
                     /gene="tcyJ"
                     /locus_tag="OO470_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6249806.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21212..21763)
                     /gene="fliZ"
                     /locus_tag="OO470_000437"
     CDS             complement(21212..21763)
                     /gene="fliZ"
                     /locus_tag="OO470_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6249807.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21809..22528)
                     /gene="fliA"
                     /locus_tag="OO470_000438"
     CDS             complement(21809..22528)
                     /gene="fliA"
                     /locus_tag="OO470_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6249808.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22693..24345)
                     /gene="fliC"
                     /locus_tag="OO470_000439"
     CDS             complement(22693..24345)
                     /gene="fliC"
                     /locus_tag="OO470_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6249809.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24611..26017
                     /gene="fliD"
                     /locus_tag="OO470_000440"
     CDS             24611..26017
                     /gene="fliD"
                     /locus_tag="OO470_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6249810.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26042..26452
                     /gene="fliS"
                     /locus_tag="OO470_000441"
     CDS             26042..26452
                     /gene="fliS"
                     /locus_tag="OO470_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6249811.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26452..26817
                     /gene="fliT"
                     /locus_tag="OO470_000442"
     CDS             26452..26817
                     /gene="fliT"
                     /locus_tag="OO470_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6249812.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26895..28382
                     /gene="amyA"
                     /locus_tag="OO470_000443"
     CDS             26895..28382
                     /gene="amyA"
                     /locus_tag="OO470_000443"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6249813.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28416..28829)
                     /locus_tag="OO470_000444"
     CDS             complement(28416..28829)
                     /locus_tag="OO470_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6249814.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29016..30221
                     /gene="yedE"
                     /locus_tag="OO470_000445"
     CDS             29016..30221
                     /gene="yedE"
                     /locus_tag="OO470_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6249815.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30218..30451
                     /gene="yedF"
                     /locus_tag="OO470_000446"
     CDS             30218..30451
                     /gene="yedF"
                     /locus_tag="OO470_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6249816.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30558..31228
                     /locus_tag="OO470_000447"
                     /pseudo
     CDS             30558..31228
                     /locus_tag="OO470_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31268..32476)
                     /locus_tag="OO470_000448"
                     /pseudo
     CDS             complement(31268..32476)
                     /locus_tag="OO470_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32544..33159
                     /gene="tnpA"
                     /locus_tag="OO470_000449"
                     /pseudo
     CDS             32544..33159
                     /gene="tnpA"
                     /locus_tag="OO470_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            33487..34569
                     /locus_tag="OO470_000450"
     CDS             33487..34569
                     /locus_tag="OO470_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6249817.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(35058..35856)
                     /locus_tag="OO470_000451"
                     /pseudo
     CDS             complement(35058..35856)
                     /locus_tag="OO470_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(35866..36417)
                     /gene="ybcL"
                     /locus_tag="OO470_000452"
     CDS             complement(35866..36417)
                     /gene="ybcL"
                     /locus_tag="OO470_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6249818.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(36586..36918)
                     /gene="emrE"
                     /locus_tag="OO470_000453"
     CDS             complement(36586..36918)
                     /gene="emrE"
                     /locus_tag="OO470_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6249819.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(37263..37577)
                     /gene="fliE"
                     /locus_tag="OO470_000454"
     CDS             complement(37263..37577)
                     /gene="fliE"
                     /locus_tag="OO470_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6249820.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            37791..39456
                     /gene="fliF"
                     /locus_tag="OO470_000455"
                     /pseudo
     CDS             37791..39456
                     /gene="fliF"
                     /locus_tag="OO470_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            39449..40444
                     /gene="fliG"
                     /locus_tag="OO470_000456"
     CDS             39449..40444
                     /gene="fliG"
                     /locus_tag="OO470_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6249821.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            40437..41123
                     /gene="fliH"
                     /locus_tag="OO470_000457"
     CDS             40437..41123
                     /gene="fliH"
                     /locus_tag="OO470_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6249822.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            41123..42496
                     /gene="fliI"
                     /locus_tag="OO470_000458"
     CDS             41123..42496
                     /gene="fliI"
                     /locus_tag="OO470_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6249823.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            42518..42859
                     /gene="fliJ"
                     /locus_tag="OO470_000459"
                     /pseudo
     CDS             42518..42859
                     /gene="fliJ"
                     /locus_tag="OO470_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            42856..43983
                     /gene="fliK"
                     /locus_tag="OO470_000460"
     CDS             42856..43983
                     /gene="fliK"
                     /locus_tag="OO470_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6249824.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            44088..44552
                     /gene="fliL"
                     /locus_tag="OO470_000461"
     CDS             44088..44552
                     /gene="fliL"
                     /locus_tag="OO470_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6249825.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            44557..45561
                     /gene="fliM"
                     /locus_tag="OO470_000462"
     CDS             44557..45561
                     /gene="fliM"
                     /locus_tag="OO470_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6249826.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            45558..45971
                     /gene="fliN"
                     /locus_tag="OO470_000463"
     CDS             45558..45971
                     /gene="fliN"
                     /locus_tag="OO470_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6249827.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            45974..46339
                     /gene="fliO"
                     /locus_tag="OO470_000464"
     CDS             45974..46339
                     /gene="fliO"
                     /locus_tag="OO470_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6249828.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            46339..47076
                     /gene="fliP"
                     /locus_tag="OO470_000465"
     CDS             46339..47076
                     /gene="fliP"
                     /locus_tag="OO470_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6249829.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            47086..47355
                     /gene="fliQ"
                     /locus_tag="OO470_000466"
     CDS             47086..47355
                     /gene="fliQ"
                     /locus_tag="OO470_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6249830.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            47364..48149
                     /gene="fliR"
                     /locus_tag="OO470_000467"
     CDS             47364..48149
                     /gene="fliR"
                     /locus_tag="OO470_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6249831.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            48434..49057
                     /gene="rcsA"
                     /locus_tag="OO470_000468"
     CDS             48434..49057
                     /gene="rcsA"
                     /locus_tag="OO470_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6249832.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(49101..49289)
                     /gene="dsrB"
                     /locus_tag="OO470_000469"
     CDS             complement(49101..49289)
                     /gene="dsrB"
                     /locus_tag="OO470_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6249833.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            49452..49679
                     /gene="yodD"
                     /locus_tag="OO470_000470"
     CDS             49452..49679
                     /gene="yodD"
                     /locus_tag="OO470_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6249834.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            49977..50792
                     /locus_tag="OO470_000471"
                     /pseudo
     CDS             49977..50792
                     /locus_tag="OO470_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(50789..52483)
                     /gene="yedQ"
                     /locus_tag="OO470_000472"
                     /pseudo
     CDS             complement(50789..52483)
                     /gene="yedQ"
                     /locus_tag="OO470_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(52654..52836)
                     /locus_tag="OO470_000473"
     CDS             complement(52654..52836)
                     /locus_tag="OO470_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6249835.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(52915..53832)
                     /locus_tag="OO470_000474"
     CDS             complement(52915..53832)
                     /locus_tag="OO470_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6249836.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            54005..54925
                     /gene="yedA"
                     /locus_tag="OO470_000475"
     CDS             54005..54925
                     /gene="yedA"
                     /locus_tag="OO470_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6249837.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(54914..55384)
                     /gene="vsr"
                     /locus_tag="OO470_000476"
     CDS             complement(54914..55384)
                     /gene="vsr"
                     /locus_tag="OO470_000476"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6249838.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(55365..56783)
                     /gene="dcm"
                     /locus_tag="OO470_000477"
     CDS             complement(55365..56783)
                     /gene="dcm"
                     /locus_tag="OO470_000477"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6249839.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(56850..57390)
                     /locus_tag="OO470_000478"
                     /pseudo
     CDS             complement(56850..57390)
                     /locus_tag="OO470_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
BASE COUNT        14450 a        14709 c        13856 g        14493 t
ORIGIN      
        1 aagtccttac aaatagaagg gggtctttac acttatctaa gatttttcct aaatcgacgc
       61 aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg
      121 tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc atacttttgt
      181 tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac
      241 aggaataatc gaaatgggat gttgcgcacg gttaaaataa ctcaccgtaa ataatcatct
      301 gctataaata atcactttca tgcaatacca gataagctat ttttaaacag acacttaccg
      361 cacaacaaac taattaaata caaagaaagg caacaatcaa cttaatgtta ataaaatgtt
      421 tttcatatat taaccacttg tttgatgcgg ggaatcagta cattattttc ttataattac
      481 agttgaaatc atatgatcac cgagagtgat tgattaattg tctttactaa taatcagact
      541 aatatttacc tgtttgactg agttgggatt gcgtcgtttc tccattagga gtaaagcttt
      601 aatgtcacct gaagttcaca gcagaataag gaacaggtta tgagctatag caatattctt
      661 gtcgctgttg cggttacacc ggaaagtcag caactgctgg caaaagcggt atctatcgcc
      721 aggccagtaa agggacacat cagtttaatt actctcgctt ccaacccgga aatgtacaat
      781 caattagccg cgccgatgct tgaagattta cgcagcgtga tgcaagaaga aacgcaaagc
      841 tttcttgata agttaattca ggatgcgggg tatcccgttg acaagacgtt tattgcctac
      901 ggtgagttaa gcgaacatat tctggaagta tgtcacaaac atcatttcaa tttggtgatt
      961 tgcggtaatc acaatcacag tttcttttcg cgagcatcct gctcggcgaa aagagtgatt
     1021 gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg gagattaacc gctcctacgc
     1081 aagctttgga aaggtagcaa ctttatcgcg ctgctggctt tccgcgcttc gcggaactat
     1141 ctgctttagg tcgctaatga agcactcctg ccagtggtta atatcgtttt tcacgataac
     1201 gtccagcatt tctgcatgac gggaaatacg ttccaccagc gacatagtca atgcacgatc
     1261 cagcgcagct gcaacttcgt cacgatcgta ggggttaaca attaacgccg acgttaactc
     1321 gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc gggttggctg ggtcctgagc
     1381 agcaacatac tcttttgcta ccaggttcat cccgtcacgc agtggcgtca ctaagcccac
     1441 gtcagagtag cggaatattt tcatcagtaa tttacggtca aaatgctgat tcaaataata
     1501 aagcggcgtc cagcctaatt gcccgtattt accattaatt cgtccagctt cattttcgag
     1561 ctgatgacga atatcctggt aggcttgcac atcaccacgc gacgttggtg caatctgggt
     1621 ataacgaatt ttaccatgat gctgcggata tttttccagc aatgcctcat aggcgagaaa
     1681 acgctccggc aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt
     1741 tttcaattcc gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc
     1801 tatttctttc ggttcaatgc cgatcgggta gacttctgtt cgaaatgctt ttccccaggc
     1861 tgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg ttagaaaggc aatccaggaa
     1921 cgccagacga tcgttttctg tctgtaaacc cagcaaatca taatcacaaa gctgttcaag
     1981 caaggtgtca tatgtcggca gcgcgttgaa aatttccggt gtcgggaaag gaatatgcaa
     2041 gaaaaaacca atgcgattat tcaccccacg tttgcgtaat tcatgcgcaa agggcaacag
     2101 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
     2161 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
     2221 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
     2281 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tagcggctga
     2341 tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgcggc tttcagtgcc
     2401 cccagtatgc caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc
     2461 cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg
     2521 ttgtaatgcg gcggttatca tttcaagcca gctccagaca tccggcacac ccgccagtcg
     2581 ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac
     2641 gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat
     2701 aaaaggagct tcctgcataa aggctgcaat cgcttcacct ttactggtac ctctcggttt
     2761 gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg
     2821 ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa
     2881 agccatccct ttcgcctcca gctccgcgcc gggatactga gcgatgaccg tatgcagttg
     2941 cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt taccattgat
     3001 gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc
     3061 gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc
     3121 cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt
     3181 gatttccgcc agcgttccat caagatcaaa aaaccaggca tatttcgcgg atagttcagg
     3241 ggtttcggtt aacggttctg tcacccggct ctcctccttc tcttttctta ttgtgttgcg
     3301 cagaccgcgc tttacagaca tgtaagtata gacagtgtga cggaggtcgc catttgcaac
     3361 gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta aattgttgtg
     3421 gaaaaaagca tcagacagtg cgcttcgctt tttgcttgta acggtcgaag atcaccgctg
     3481 ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca
     3541 tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga
     3601 tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga gataacaata agctcataac
     3661 caatcgacgt cattggctgc ccactagtca tacgtgaagc cagaataatt ccggctatcg
     3721 cagataccag gccagagaga acaaagataa taattttggt gcgaacaacc ggtatacccg
     3781 ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt attacgacca aaggtggttt
     3841 tattcagcag caaaccaaag ataatcagac acgcgacggt gagccagatt ggcgcaggca
     3901 gaccgaacca gttagcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt
     3961 taccgtctga aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgtcgtga
     4021 tcagagcatt tattttcagt ttggcgataa caaagccatt gaccaggcca cagagaacgc
     4081 ccagcaacaa acccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg
     4141 ccgtggtgac acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag
     4201 caaggcagaa caacatgcca cacgccacca tccccgacat ggaaattgcc aggcccaacc
     4261 ctttcatatt aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca
     4321 ccgcaaacac caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact
     4381 taggtgcgcc agaccccgat gtagaaacag aagacatcat actctcctta ctcaggcaac
     4441 tgcctggctg actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg
     4501 taacaattca ccggcgattt cgccttcccg catcaccaca atccggtcgg caacgccgag
     4561 gacttcaggt aagtcgctgg aggcaaacag caccgccacg ccctgcgccg ccagcgcata
     4621 aattacgtta tatatttcgt gcttagcgcc aacatcaatg ccgcgcgtag gttcatccag
     4681 caaaatgacc ttcatctctt ccgataacca gcggcccaga atggcttttt gctgatttcc
     4741 gcctgagaga ttcatgatca gttgctccgc gcccggcgtt ttgatgttga gcgaacgaat
     4801 gtgctgatcg gcattgtttt cttcccaact gttgttgatt acacaaccgc cgagcacatg
     4861 tttgcgtctg gcactgatgt tgatattgtc gcgaacggag tgcacgggaa taatgccttc
     4921 cgctttgcga gcttccgggc agagcatcat gcctgcggca atggcgtggc tcggtttacg
     4981 aatatcgatc ggctgttggt cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa
     5041 caagcctttc attaattcgc tacgccccgc ccctaccaga ccaaacagcc caacaatttc
     5101 accactgcga accgccagac ttattggcgt acgcacgcct agtgctttca cagcatcaag
     5161 acgcaggcgc tcctcgccat aactacgagg ttgccagccg tagatatcgc caatgtcgcg
     5221 cccgaccatc gcctgcacca gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt
     5281 gacataacgt ccatctttaa agacagtgat ggcatcgctg agggcaaata tttcttccat
     5341 acggtgagaa acgtataaga tcacccgccc ctcttttcgc agttcacgaa taacgcggaa
     5401 aagattgccg atttcgcggg cagaaaggga gctggttggc tcatcaaagg cgataatttt
     5461 agcgttacgc gccagcgctt tggcgatttc aaccatctgc cactgaccaa tggcgagata
     5521 tttcagcggc gtgtccgggt caatatccat accaagatgt ttaagttgta aacccgcctc
     5581 ataattcagc aatgagcgat tcacaatgcc gcctttatgc ggcagctggc cgagatagat
     5641 gttttccgcg acggtcattt ccggcacgag atgcagttcc tggtaaataa tcgccacacc
     5701 cgcgttaagt gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa tcactacaga
     5761 acccgtggtt ggcgcatagt tgccgctgag gatttttaag agagttgatt ttcctgcgcc
     5821 attttcaccc atcaacgcat gaacctgacc ggcatagcag tcaaaactaa tatccgtcag
     5881 cgccttaacg ccgggaaacg ttttaccgat gccgcgaaat gagagatacg gggtagactg
     5941 ttgcataacg tctccgtgaa tcactggtcg ttcacacccc ctctgcatca tgcagagggg
     6001 aatttttccg gcaaattact taccgcctaa accttttttc tccagttctt ctttaaagtt
     6061 gtcacgcgtg atcagtacca cgtctgtaac ttcggtaaat tttggcgttt caacgtcttt
     6121 tgctacccag ttgtaaagca tttcgctgga tttatagcca tgtacgtccg ggcttggcag
     6181 cagggaaccg tagaagccgg ttgcctgtgc tttagacagt tcgcttactg catccacacc
     6241 gttaatacca ataccgatga tatcggctgc tttaaagccc tgaccttcgg ttgcacgtac
     6301 gccgccaagt acggtgctgt cgttcatacc gacgatcagc caatgtttaa cttccggatg
     6361 ttgaaccagc attgagttgg cagcgtcaaa tgcccccggg atgtcgttag atttggtagg
     6421 tacctgataa atttgttttt ccgggaatcc tgcagctttc agtgcgtcca tagaacccgt
     6481 tgtacggcgg cgggcggtat ccagttcgtt ggcggtaatc gccatcactg cgctttcttt
     6541 gacatcccag ccacgtttct gcatctcttt atacagttcc tagccctgac gttcgccaat
     6601 tttagtcgcc gccatcatca ccagaggaac ggtatccatt ggcttacctt tggcgttaac
     6661 aaactggtca tccactgcaa tgactttcat atcgtagcca cgcgctttcg cgacgatggc
     6721 agagccgagt ttcgggtccg gagtacaaat aacgaaacct tttgcgccac tggctgccag
     6781 gctgtcgatc gcgttcaatg ttttttcgcc atccggcacg gcaatcttaa taacctcaaa
     6841 ccctaaatcc ttcccggctt tatcggcaaa cttccattcg gtctggaacc acggctcttc
     6901 cggttgcttc accagaaaac cgagcttcag gttctccgcc atagcggatt gtgacataac
     6961 ggctgccaga ccaatggctg ccagggcttt agtaaatttg tgcatggttc tctccagctt
     7021 tagtgtcgtt ttgtgtagtg caaaaacgaa tgacattcgt taaattaatc ggaaaacaaa
     7081 gcattacctt ttatctaaaa gataagtgac cgtgttgaca tagttttagc gagaaattaa
     7141 ttctccatag gagagcaata tcacatcgca gaattacagt gagaacgtgc ataaatttag
     7201 cgggaaaaga cataaggaaa agccaatttg tcagacaaat tgttgaatgc acagcagatt
     7261 aatccataag attagcctgg aaatccttgt tgtcttcggt acccattcgg gatgtcttct
     7321 ttttaaccag tcaataggcc gcattacctg gcgttgagtt tttgaaatgg tgtaataacc
     7381 acaactcaaa gatgtggaaa atgcgcgtca ttcatttcgc tattaattat cactgtgctc
     7441 attaattaac agaacacgta taatgagagc catctcgcaa aaatgaaaaa acgttttata
     7501 aaatcatcac ttcatcatga attcaaattc atcgattaat atcaacaaga tacaaaaagc
     7561 actatcatta aaattcattg catttacatt gatttcacca atgaaatgta aaaatatata
     7621 aacttgatga tttaagcatt ttcttataac cgttcagacg ttattcttat ttcagatcat
     7681 cgtcagaatt gactccacga tcacatttcg gaccggcaga aaggaattat tctgcaaaca
     7741 gtaattatgg tgttttgatt tatcttgcac ctctccactt ctggatataa ggatattagg
     7801 tatggcaacc gctggaatgc ttctcaaact caactctcaa atgaaccgcg agttttacgc
     7861 atccaatctc taccttcacc tgagtaactg gtgttctgaa cagagtctga acggcaccgc
     7921 cactttcctt cgcgcccagg cacagagtaa tgtgacccaa atgatgcgca tgtttaactt
     7981 tatgaagagt gtcggcgcta cccccatcgt taaagccatt gatgttccag gtgaaaaact
     8041 gaactctctg gaagaactgt tccaaaaaac gatggaagaa tacgagcaac gttcaagcac
     8101 tctggcgcag ttagccgatg aagcgaaaga actgaatgat gattcaaccg tcaatttcct
     8161 gcgcgatctg gaaaaagaac agcagcatga tggcctgttg ctgcaaacca ttcttgatga
     8221 agtgcgcagt gcgaaacttg cgggtatgtg ccctgtgcag accgaccaac atgttctgaa
     8281 tgtcgtgtca caccagctgc attgatcata catcggcgct aatacattgc gccgatgaag
     8341 tttttgagaa accgctgcct catctgtttg aagcagcggt ttttttaatg ggattcaccc
     8401 tgtggggtaa acttgagttc aatcagcgcg atggcttttt ggattgcccg catggtgacc
     8461 gggtctgcgg cagcgggatg atgagtaaag tcgatattct tcagctgact ggacattttt
     8521 tcacgaactt caacgggcgc gattacatcg agaacatcca gaatttgttt gataaccaac
     8581 tggcaagcta ccacatcaga aaccagttcc tgatcggcat tcagcggctg ggacatcgta
     8641 aactcctgat agcattttga aagccgttat agtagcggct tcacatcttc agcgatagtc
     8701 acatccaccg tcatcaggac acaaaaaaac ctgccggagc aggttttttg ttatcggaac
     8761 atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt tattgaacat acttttcagg
     8821 attttgcgca gtttcatcgc ggcactccga ccatttgtta tacaggtgtt attgtctttg
     8881 cgcttataat atgacaacca tcacaaaaat caatctttat gtgatacaaa tcacataaat
     8941 acccctttaa tgttataaaa atgataatca aaaaacagcc cccctatttc tgacacctac
     9001 agatggcaag aaatagcgcc tgccaggcgt cttttccggc cattgtcgca gcgctgtaac
     9061 gcgtaaaata gtgctttctc ttactcttct ggctggacca tgagacttct gattctgact
     9121 ctttcattaa taacgctggc ggggtgtacg gttactcgcc aggcccatgt gagcgaagtt
     9181 gacgcggcaa caggtattgt acggttggtt tatgaccagg cttttttgca gcatgcccat
     9241 accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat gccagcagga aggctatacc
     9301 cacgcgatcc cctttggtca gccagtaggc aactgtagcc tttttgcggg ttccttatgt
     9361 ttgaatacgg aattcacttt gtcatatcag tgccatcatt ccgccttccc tgtctttctc
     9421 taaatatttt aatcagcgag gggatcttcg ctgattaaag aaatagcggt aatgcgtttt
     9481 attcccattc gtatttttaa taattgaagt ttatatttta ccttttgcaa ataataaaat
     9541 aacaaattat agtgacgcca cagcaacaaa tataaccttt gtggagcact atcatgctga
     9601 aaccagaaat gattgaaaaa cttaatgagc agatgaacct ggaactgtac tcttcactgc
     9661 tttatcagca aatgagcgcc tggtgcagct atcatacctt cgaaggtgct gccgcgttcc
     9721 tgcgccgtca cgcccaggaa gagatgacgc atatgcagcg tctgtttgat tacctgactg
     9781 ataccggcaa tttaccgcgt attaataccg ttgaatctcc gtttgctgaa tattcctcac
     9841 ttgatgaatt attccaggaa acctataaac acgaacaatt aatcacccag aaaattaacg
     9901 aactggctca tgctgcaatg accaatcagg actacccaac atttaatttc ctgcagtggt
     9961 atgtttctga gcagcatgaa gaagagaaac tgttcaaatc gattattgat aaattaagcc
    10021 tggcaggcaa aagcggcgaa ggtctgtatt ttatcgacaa agaactctct accctcgaca
    10081 cacaaaacta atgctctccg gcggcaggtt tctgccgccg tttcaaatca gtgacggcaa
    10141 atcttactct gatcggtcga aaaaccttct tctgaaggta taaatttgcc ttttgctgcc
    10201 agaaacgcta ccagctctcc cgctgtcacc ccttctgccg agcacgtgtg aaaacgtgcc
    10261 tgttcaccaa aacgcgcttt aatcgcagct tccagactgg catgcgtata ttgctcgcct
    10321 gattcaatca tcatatttaa cacttcatga ccgtgaatag agtccatcgt tcctcctcaa
    10381 aaaaagccta gcgtagcgat taccgcttat gaagactttg cgccagcgca ggactgaatg
    10441 cttttattgt acatttatat ttacacaata tgtaacgtcg gtttgacgaa gcagccgtta
    10501 tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga
    10561 aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc
    10621 tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg
    10681 ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc
    10741 cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcaatggc
    10801 tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcagcatac atcagcggtg
    10861 ccggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg
    10921 ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg
    10981 tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg
    11041 tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc
    11101 tggcactgtc tgcaatcccg gtgattttta cgtcgtttgg ttttcacggt agcgtgccga
    11161 gtattgtcag ctatatggat ggcaacattc gtaagctacg ctgggtgttt ataatcggta
    11221 gtgccatccc cctggtggca tatattttct ggcaggtggc gacgcttggc agcattgatt
    11281 caacaacctt tatgggattg ctggctaatc atgctggatt aaacgggctg ttacaggcgt
    11341 tacgcgaaat ggtggcctct ccgcatgttg agctggcggt gcatttattt gctgatttag
    11401 ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttgt
    11461 ttcagcgttc aaataccgtt ggtggacgat tgcaaactgg tgcaattacc tttctgccgc
    11521 cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg
    11581 tggcgctggc ggtactggca ttgattatcc cttcactatt gacctggcaa agcagaaagc
    11641 acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc
    11701 tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcaggcttg ttaccagaag
    11761 tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa
    11821 tgtgacctct taatgcaggc agcactgctt aaatttctta ccactgccgc acggacaagg
    11881 atcgttacgc cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg
    11941 atgcgccatc cagtatgcat gtaaatcaag cgccgccagt cgaatggcat ctacgctctc
    12001 ttcaaacgct tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag
    12061 cgcaatcgcc tccagcgctg gttttaaaga atcgggcaac gttgaccagt cagaaagtgc
    12121 cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac
    12181 ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt
    12241 atcggccata tgttgaaaag ccaggttcat aaagcgcgtc atctctttct ctgacgccca
    12301 acgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttctg gttcaatctc
    12361 ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc
    12421 gtggtcagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa
    12481 cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat
    12541 gccggtccgt atcagagata ctttttgagt ggctttgccg gtgattaaaa attaaggagg
    12601 gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt
    12661 attactttga ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac
    12721 agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg
    12781 ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta
    12841 cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac
    12901 gccgatgtct tttaaactgg agcgggaaac gagactcgaa ctcgagaccc cgaccttggc
    12961 aaggtcgtgc tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt
    13021 gattttatta tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg
    13081 gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa
    13141 acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca
    13201 gagtcagtac cgcagccaca aagaaaagtg caataccgac gtactcaacc caaatgttcg
    13261 gacgccacag cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc
    13321 aggagacggc cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa
    13381 taataatttc acgggcgatc atcgttgccg ccggtaatgt cacccaccag ctgtgataat
    13441 gctcggttac cagcaccatg gcgatagcca cgagaacttt atctgccaca gggtcaagga
    13501 aagcaccaaa ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag
    13561 tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat
    13621 aaaagaccaa tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga
    13681 tattaaattg cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct
    13741 acagagacat caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc
    13801 cggcactttt gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata
    13861 tttcaacaac atttgccgac gttttggccc gacgccttca atggtttcca gggaactggt
    13921 atttttgacc ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcat gtgattcatc
    13981 gcgaatatgc tggataacat gcagcgcggg tgaatctggc ggcaaactaa atccctcacc
    14041 ttccggctca aagaacagcg tttccagtcc agccttacga tctgctcctt tggcaacgcc
    14101 aagtagcagc ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc
    14161 ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc
    14221 aatggcttta ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg
    14281 cgtgatgcct gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa
    14341 caccacacag gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat
    14401 ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt
    14461 agattgctgc gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag
    14521 ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc
    14581 tgaaagggaa tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc
    14641 cggtaaggtg cgcatctggc tgccttgtaa atagaactga cctacgaagg tttctaccac
    14701 ctcgctcagt tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt
    14761 gccctgacga atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat
    14821 aacgtcaagg tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc
    14881 ctgaatttgg tcgcgaatac atgccgcttc ttcaaactcc agattctggc tggcagtttc
    14941 catacggcta atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac
    15001 atactcgacc tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag
    15061 acagcgccct atctggtatt gcagacacgg acgcgagcga ttgcgataaa cactattttc
    15121 gcactggcga atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc
    15181 attcgggaac gggccgaaat attcaccttt ggcatgcttt gcaccacgat gcatcgccag
    15241 acgcgggtgg gtatcgccac tcaggaagat aaaaggataa gatttatcat cgcgtagcaa
    15301 aacgttgtaa cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt
    15361 ttctgtatgc gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt
    15421 gcgcgaagcg aggttgctgc ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt
    15481 gccgacatag ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt
    15541 tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc
    15601 gcattacaca gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt
    15661 agtttactga acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt
    15721 tctgagatct cattgacctt ctggccctta gtgatcatca gcataatctg caattcacgt
    15781 tcagacaaac tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt
    15841 tgttgagcga tatcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc
    15901 acgacttcct gcggagccgc gcctttgctg aggtagccag cagcacctgc ctgcatgact
    15961 ttcgctggta aagggttttc tgtatggacc gtaagcatga tgattttaac atcagctgtg
    16021 gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt
    16081 agcaccacgt caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc
    16141 tctccggcga cttttatacc cttaatatct tccagaatgc gtcgtatccc tgcgcgcacc
    16201 agttcgtggt catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata
    16261 acgctactga tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta
    16321 aaaatggtgg gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg
    16381 tcacgcccgt caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg
    16441 acaaaaattg actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat
    16501 taacccgaac aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt
    16561 aacgtatttt atagcactgc cgatatcacg ctcaaaaaac aaccactgct atttttagca
    16621 aagcttatgg tatactccgc cgccttaaca tttttcacca caaattttca ttgcaacatg
    16681 acgaggaaaa taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc
    16741 aaatgaagtc tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc
    16801 tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac
    16861 ccaggctgca gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata
    16921 taaaaccggc tgagagcgtg cctttcagcc ggttattgac aaaatcatcg ctatctctgc
    16981 tgatgtcttc ccttccgccg tccatttcag aatttctttt tcgcgcttgc tgaaattcat
    17041 ctctggcgtc atcactattt catcatttaa acgcatcaga gccatcagac tttcgcgcac
    17101 cagtaactgc atttttaatt gcagttcatc actaagaatg ggtatttcgc gcgtgctgca
    17161 acgggaaaag gacaaaaagc ccagcgcccg atttggcagc attaaatact gagtgacacc
    17221 gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc tgtgcttcgc tgaataagtc
    17281 atcattccac attaaatggc cctgactaaa gttttcaggg ttcagcacag gatcaattgc
    17341 gagaaaattt tttgcctgat aataactaac ccacgactca gggtaattgg tgtagaaagc
    17401 cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat aacgaatagt aatcgtactc
    17461 cagctgctga gcctgaagct ctatttcatg gtaggcctct tctgcggcct ccatcctctg
    17521 aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc gtatcctgca tagtaaaccg
    17581 caacgcccct gagagtgagt atcatttata atgataattc aaactatcac ataaataaga
    17641 tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat cgcattctct
    17701 tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc
    17761 ccgccccgac aggtgaatcg tcggggctga ttttttctta ttattgcagc agaagcttct
    17821 cgaggaactg gcgggtgcga ggctgctggg gatcggcaaa taacgctttt gctgccccct
    17881 gctcgactat ccgcccctgg tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc
    17941 tcatttcgtg tgtcacaatc accatcgtgc gcttttcctg cgccagctgg cggatggtgt
    18001 tcaggacttc gcccaccagc tctggatcca gcgccgacgt tggctcgtca aacaaaatca
    18061 cctcaggacg cattgccagt gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca
    18121 aacgacgtgg atagctggtt tctttaccgg ccagcccaac tttcgccagc agctcgcgag
    18181 cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct tcaataatgt
    18241 tttccagcac cgtacgatgc ggaaacaaat taaagttctg gaagacaaac ccgacgtgct
    18301 gacgcaactg gcgaatcagc gatttttgct gacttaatga acgtgcagta tcaatagtga
    18361 tatcgccaac ggtgatcgtt cccgcttccg gttgttccag cagatttatg ctacgtagca
    18421 atgtggtttt gccggaacca ctcggaccga taattgccac cacttcgcca ggctttacct
    18481 cgaggtcgat accgtgcagc accgtctgac cgtggaattt tttcaccagg ttcttaactt
    18541 caatggcact cattttggtt ctctctcctg gcgattaagt tgattctcaa aatggttctg
    18601 caacgtcgat aacacagtcg ccatgatcca gtagatgagc gaagccgcca ggtacatggt
    18661 gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct ccggcacctg
    18721 gattgtcgcg gccagcgacg tatcttttac cagactgata aagctgttcg acagcggcgg
    18781 caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat
    18841 accaatactg gcagcggctt cccactgacc tttatcaata gaagaaatgg cggcgcgcag
    18901 cgtttcggcg gcataggcgg cagtgttcag cgacagacca atcattgctg acggaatggg
    18961 atctaattcg ataccaaact gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag
    19021 cggcgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg gccagattgg
    19081 cgacaggcgc atcagcgcga gaataaaccc gagtagtaag ccaaaaaaca tgccgccgat
    19141 actgagttgc agggtatacc cagccccttt caacaggaac ggcaaggaat caataaccag
    19201 ttgtatactt tcttgcatga gcgttcccgg agctaaacgt ggggatgata ggcgaacagc
    19261 gcaggcgcgc cgccggtatg aataaacaga atcggccctt catctttgaa gcgtttctga
    19321 ctgataccgt caatcagccc cgccatcgct tttccggtat acacaggatc aagcagaatg
    19381 ccttccagac gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg cacgccgtag
    19441 ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg atgcggtcag ctccagttct
    19501 ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt gatcggcaac ggaacgcgac
    19561 acggtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac agccagtccg
    19621 gcgtgagttc cggcactgcc cgatgcgact accaccgacg aaatattaac cgccccttca
    19681 cactgttgcg cgatttccag cgcactctcc acataaccta gcgcgccaag agcattagaa
    19741 ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt cgccagttct
    19801 tccagttggg cattgggatc ggtcagtgcg tcgcacattt caatctgggt attgaacaga
    19861 tccaacaaca aacgattgcc gttggttaaa tagttttctg cggttgtgcc aataggattt
    19921 tccagcagcg ccacgcagtg cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca
    19981 tggttagact ggatcgcgcc ggcagtaatc agcgtatcgg caccttcgcg cagggcatct
    20041 gccgcgagaa attccagctt acgtaattta ttgccgccca ttgccatggg ggtgacctca
    20101 tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag atattcgagc
    20161 ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg ttaaattatg cagtggcata
    20221 acagcctccg atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata
    20281 aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc tgattatttg gtcacatcag
    20341 caccaaacca tttttcggaa agagcttgca gagtgccgtc tttttgcatt tccgcaattg
    20401 catcattcac cgctttcagc aggtcttcat ttcctttacg cagcgccacg ccagactcct
    20461 gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc accagatcca
    20521 gcgccgccag acgatcaacg aggatcgcat cgatacgccc tacgcgcaga tcctgatatt
    20581 tggtcgggtc atcatcatag gtacgcacat cgacaccctg aacattctgc cgcagccact
    20641 cttcatagtt ggtgcccaga ccgacaccca cttttttgcc tttcagatca gcagccgttt
    20701 taatggtgcc ttcgttacct tttttcacca gcgcctgaat accggaaatg gtgtaagggg
    20761 tagagaaatc gtattttttc ttgcgctcgt cagaaatggt gacctgatta atcaccacat
    20821 caatgcgttt agagtccagc gacgccagca taccgtccca tttggtcggt tttagtgacg
    20881 cctcaacgcc aagatgtttt gccagctgtt gggcaaattc cacttcaaaa ccggttaatt
    20941 tgccgtcatc cccctgaaaa ctgaacggcg gataagtgcc ttccagccct accagcagcg
    21001 tgccgctctc tttgacttta ttaagcagac cttcatctgc aaaactttta acgctcatgc
    21061 ccgcaaccag cgccacggcc atcacaccca tcaatgcctg acgtcccaga tgtgctaatt
    21121 tcatattcac cccgaatgtt gttatgtctg tttgcagtgt agagccattg tttgtaaaca
    21181 caaaaacaac tccgctacat cttattctta tttaatatat atcagaagca ggcaggctgg
    21241 aggatttctg cacaagtccg gtacaggttt ggcgctgata gtgttgatac ttccgtaatg
    21301 cgatgcggta attgttggtg ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga
    21361 aaccgtcctg cagctgatcg agggaaatat tttgctcatg tagatgatta cccaaacggc
    21421 gcagacgaac gacatattca cgcaccgtac ctgggctcat ttcagtttgc tcaaaaagaa
    21481 atttcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc
    21541 aaaatcggca caatgccgcc catgattgtt gttccgtttg ccagcctttt tcatcaagca
    21601 gcgtgtccag gcgggaaatc tcgattttat tcactatctt gccgtcgcga actaaggtaa
    21661 tgcgatcgag taatttacgg caatgcgcgc aatgggtctg gctgtgttta aagtctttaa
    21721 gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac tcctggtagt
    21781 caaagttaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg cgtaaccgtt
    21841 taatggcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc accgcgccaa
    21901 tctctttgag attcagctct tcctggtaat agagggttaa taccagtttt tcgcgctccg
    21961 gcaacgtttc gatggcttcc atcacccgct ggcgcagatt actgtccagc agttgttgta
    22021 gcgggttttc tcgctgatga tcatcagtaa ccagttcgat gctatcgccg tgctcttcac
    22081 gccactcatc gtaggagaag agctggctgt tattggtgtc gagcagcatt tgacgataat
    22141 cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg cggccaagtt
    22201 cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg
    22261 gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc tgcactgcgt
    22321 aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacagcatta agtaacccaa
    22381 tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc
    22441 gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt ttatccatta
    22501 caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat gagttatcgg
    22561 catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa atgaggggtt
    22621 atttgggggt tacgcaaaaa gccgaatgta aaaaaccccg ccggtggcgg ggtttgagag
    22681 gcattcagac gattaaccct gcagcagaga cagaacctgc tgcggtacct ggttggcttt
    22741 tgccagcacg gagttaccgg cctgctggat aatctgcgct ttcgacatgt tggacacttc
    22801 ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca gacaggttgg tagtggtgtt
    22861 gttcaggttg gtgactgcgg aatccaaacg gttttgcacc gcacccaggg aggagcggaa
    22921 tttgtcgatg gagctgatgg cttcatccag agctttcagg ggatcggctg tcgtagctgc
    22981 ttttgtttca gcatctgtcg tcaatttacc atcagcatct ttataaactg ccttaccgct
    23041 gccgttagtc actgaaccat catcctgtag gtaaaaatct acagtatcag atgtggtcaa
    23101 cgcctcgttg ttatcaacat aaaccgaatc aacacctttt gttacagcgc cagttgtgcc
    23161 gttgacgcta taacctgcag tagtgaaatc aacattgttt cccgaggttg atacagcttt
    23221 atcgaaagct gcctgatcaa cttttgcacc tgttactgaa taagtaatag tgtccggcgt
    23281 agtactatta ccggtaaagg agatagaggt accgccaatg tcaatggttg tcgctttggt
    23341 attattggct gcaagtgtgg acagactagc ctcagtcaaa gaagcaccag agccgttttt
    23401 cgttaagcgc ccagttgtat caatgtagag tttatctcca ttgcttgccg taattttacc
    23461 atcactgctg atctgaacat cctgtgcaga accatcgata gtaatagtgc ccttagcggt
    23521 atcaccaact cccggggtca aatatttctg aacatcggca gctgaagccg ttgtggtatc
    23581 aaaagagtat gacttacttg cactgtcata cttgtaattc gttgcactag ccgccgttcc
    23641 gaagccgtta ttaatggttg cctgaacagt atcaccatca ctaactccag ccagcaaatc
    23701 agacgcttta gcagcatcgt aacccgcact cacagtatat ttgttgccga ccactgttgc
    23761 attagcagct accaagtcag ctttagatgc agcagtatta gccacagccc cgccaccatt
    23821 cacattaaac ccattcagcc ccagcgtatc agagtcaatt ttcttcagat caatagtgat
    23881 agtctggccg tcattcgcac caacctgaat tttcatcgaa ccgtcttttg ccagtacgtt
    23941 cacgccgttg aactgggtct gaccggatac gcggtcaatt tcgtcgagac gggatttgat
    24001 ttcgtcctga atggagtcca gatccgaatc agagttagtc ccggtagaag cctgaaccgt
    24061 cagctcacgg atacgctgta agttgttgtt gatttcggac agcgcgcctt cagtggtctg
    24121 tgcaacagaa ataccgtcgt tggcgttacg tgcagcctga gtcaggcctt taatgttaga
    24181 agtaaaacgg ttagcaatcg cctgacccgc ggcgtcatcc ttcgcgctgt taatacgcaa
    24241 gccagaagac agacgctcga tagaactcga cagcgcagac tggttcttgt tgatattatt
    24301 ttgagtgatc agcgagaggc tgttggtatt aatgacttgt gccatgattc gttatcctat
    24361 attgcaagtc gttgattacg ttttgggttt ctacccgtcg gctcaatcgc cgtcaaccct
    24421 gttatcgtct gtcgtaaaac aacctttaga atttttttca caaacagcca ttttttgtta
    24481 gtcgacgaaa tactcttttc tctgcccctt attcccgcta ttaaaaaaaa caattaaacg
    24541 taaactttgc gcaattcagg ccgataaccc cggtattcgt tttacgtgtc gaaagataaa
    24601 aggaaatcgc atggcaagta tttcatcgct gggagtcggg tcaggtctgg atttaagttc
    24661 aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta acccctattt caaatcagca
    24721 atcgtcgttt accgctaaac ttagcgccta cggtacgctg aaaagcgcgc tgacgacttt
    24781 ccagaccgcc aatactgcat tgtctaaagc cgatcttttt tccgccacca gcaccaccag
    24841 cagcaccacc gcgttcagtg ccaccaccgc gggtaacgcc atcgccggga aatacaccat
    24901 cagcgtcacc catctggcgc aggcgcaaac cctgaccacg cgcaccacca gagatgatac
    24961 gaaaacggcg atcgccacca gcgacagcaa actcaccatt caacaaggcg acgacaaaga
    25021 tccgattacc attgatatca gcgcggctaa ctcatcgtta agcgggatcc gtgatgccat
    25081 caacaacgca aaagcgggcg taagtgcgag catcattaac gtgggtaacg gtgaatatcg
    25141 tctgtcagtc acatcaaatg acaccggcct tgataatgcg atgacactct cggtcagcgg
    25201 tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt gccagcagca acggtatgga
    25261 ggtctcggtc gccgcccaga atgcgcagct gacggtcaac aacgtcgcca tcgagaacag
    25321 cagcaacact atcagcgacg cgctggaaaa catcaccctg aacctgaacg atgtcaccac
    25381 gggcaaccag acgctaacca tcactcagga cacctccaaa gcgcaaacgg cgattaaaga
    25441 ctgggtgaat gcctataact cgctaataga taccttcagc agcctgacca aatacaccgc
    25501 cgtagatgcg ggagctgata gccagagttc tagcaatggc gcactgctcg gcgactccac
    25561 gctgcggacg attcagacgc agttgaagtc gatgctgagt aataccgtca gttcttccag
    25621 ctataaaacg ctggcgcaga ttggtatcac gaccgatccc agcgatggca aactggaact
    25681 ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc agcggcgtag gtgcattgat
    25741 tgttggcgat ggtaaaaaaa ccggcatcac aaccaccatc ggcagcaacc tgaccagttg
    25801 gctttcgaca acgggcatta ttaaagccgc taccgatggc gttagtaaaa ccctgaataa
    25861 gttaactaaa gactacaacg ccgccagcga tcgcattgat gcgcaggtcg cgcgctacaa
    25921 agaacaattt acccaactgg acgttttaat gacctcgtta aacagcacca gcagctactt
    25981 aacgcagcag ttcgaaaaca acagtaattc caagtaatca ttattcatcg ggagacaggt
    26041 catgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcaccgtaat
    26101 gagcgccagc caacagcagt tggtcaccat gctatttgat ggagtgctga gcgcactggt
    26161 tagagcgcga ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa
    26221 agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaagaga gcaaagacga
    26281 attaacccaa aacttaattg ctctttatag ctatatggtc aggcgtttgc tgcaagccaa
    26341 tttacgcaac gatgtctccg cagtcgaaga ggtggaagca ttaatgcgta atattgccga
    26401 tgtctggaaa gagtctttac tctccccttc ttcgattcag gacccagtct gatgaacaat
    26461 gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc
    26521 ctggcaacgg aagaacaatg ggacgaactc atcgctagcg aaatggcgta tgtgaatgcg
    26581 gtgcaggaga tcgcacacct gactgaagag gttgccccgt ccactacgat gcaggagcag
    26641 ctccgcccga tgctgcacct gattctcgac aacgaaagca aggtaaagca gttattacag
    26701 attcggatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta
    26761 agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc
    26821 ttaatgagcc gatggctcgc gaataatccg attacggcta cgtttctaat attccccttg
    26881 aatggagtcg aagaatgcgt aatcccacgc tgttacaatg ttttcactgg tattacccgg
    26941 aaggcggtaa gctctggcct gaactggccg agcgcgcaga cggttttaat gatattggta
    27001 tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcggctat tcggtcggct
    27061 acgactccta tgatttattt gatctaggtg agtttgatca gaaaggcagc atccctacta
    27121 aatatggtga taaagcacaa ctgctggccg ccattgacgc cctgaaacgt aatgacattg
    27181 cggtgctgct ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc
    27241 gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga cgaagaaatc attgagtgtg
    27301 aaggctggac gcgttacacc ttccccgctc gtgccgggca atactcgcag tttatctggg
    27361 atttcaaatg ttttagcggt atcgaccata tcgaacaccc tgatgaagat ggcattttta
    27421 aaattgttaa cgactatacc ggagaaggct ggaacgatca ggttgatgat gaactcggta
    27481 atttcgatta tctgatgggt gagaatatcg attttcgcaa tcatgccgtg acggaagaga
    27541 ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggcttt cgtcttgatg
    27601 cggtcaaaca tattccggca tggttttata aagagtggat cgaacacgta caggaagttg
    27661 cgccaaagcc gctgtttatt gtggctgaat actggtcgca tgaagtcgat aaactgcaaa
    27721 cgtatattga tcaggtggaa ggcaaaacca tgctgtttga tgcgccgctg cagatgaaat
    27781 tccatgaagc atcgcgcatg ggtcgcgact acgacatgac gcagatcttc actgggacgc
    27841 tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc caaccacgac acccaaccgt
    27901 tgcaagccct cgaagcaccg gtcgaaccgt ggtttaaacc gctggcgtat gccttaattt
    27961 tgttgcggga aaatggcgtt ccttcggtgt tctatccgga cctctacggt gcacattacg
    28021 aagatgtcgg tggtgacggg caaacctatc cgatagatat gccaataatc gaacagcttg
    28081 atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacatta tttttcgacc
    28141 atccgaactg cattgccttt agccgcagtg gcaccgacga atatcccggc tgcgtggtgg
    28201 tcatgtcgaa cggagatgat ggcgaaaaaa ccattcatct gggagagaat tacggcaata
    28261 aaacctggcg tgattttctg gggaaccggc aagagagcgt agtgacagac gaaaacggcg
    28321 aagcgacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt
    28381 aaatccatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc
    28441 gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata
    28501 ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta
    28561 acgttagtga gatcatcaga catcaatgtc aacttacccg gcacggcaat cccccgctgc
    28621 cactgacggc aatccaaggt atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc
    28681 ggactgacca gcgcacgttg cggccctttg gtctgccagt agcccgccag ccagtccggc
    28741 gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg
    28801 agcgcgcctg caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt
    28861 gtggcattaa agctctgatg tcgccagcct ttccaggttc agagagaaat ttgatctaac
    28921 tattattttt gtgaattatt accccgaaaa taaatttgca tggtgtatga ttcgcggctc
    28981 ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg
    29041 cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca
    29101 cttactattt tggcattact ggcacctttt gggctgtcac gggtgaattt acccgttggg
    29161 gcggtcagct cctgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta
    29221 tccatctgga aggatcgcca ttaacccgca tcgacggcat gatgatcctc ggtatgtttg
    29281 gcggctgctt tgctgctgcg ctgtgggcca acaatgtcaa actacgcatg ccacgcagcc
    29341 gtatccgcat tatgcaggcc atcattggcg gcattatcgc cggttttggc gcgcgtctgg
    29401 caatgggctg taacctggcg gcgttcttta ccggcattcc tcagttctcg ctgcatgcct
    29461 ggttctttgc catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc
    29521 ccatcttccg tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa
    29581 aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg
    29641 gctgggcgct gctcacggcg atgaaccaac caaaactggg gctggcaatg ctgtttggcg
    29701 tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata
    29761 tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtgagtg
    29821 ccatcgggat cttcagttac gtacagttag gcgttgaacc taaaatcatg tgggcgggac
    29881 caaatgcggt aattggtggt ttactttttg gttttggcat cgtgctggca ggcggctgcg
    29941 aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg
    30001 gcaacgtgat cggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg
    30061 ccaccgactg ggacaaaatc aacctgctga aaacttttgg tccgatggga ggcctgctgg
    30121 tgacatattt gctgctgttt gctgcgctga tgttgattat cggctgggaa aaacgcttct
    30181 tcccccgtga ggcaccgcag actgctaagg agatcgcatg aaaaatatcg ttcctgatta
    30241 ccgccttgat atggtgggtg aaccctgccc ttatccggca gtcgcaaccc ttgaggcgat
    30301 gccgcaatta aaaaaaggtg agatcctgga agtggtgagc gactgtccgc agtcgatcaa
    30361 taatattcca ctggatgcgc gtaatcacgg ctacacggtg ctggatattc agcaagacgg
    30421 gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg
    30481 gaacatcgtt acctctgaca catagatttt acccgccccc tctcctattc ttttcagtat
    30541 gatctcagga ggttgctatg tgtggacgct ttgcccaatc ccaaacgcgt gaggattacc
    30601 ttgcgcttct cgcggaagat attgaacgcg atattcccta cgatcccgaa cccattggca
    30661 gatacaatgt cgcgccggga accaaagttc ttctgctcag tgaacgtgat gaacaccttc
    30721 atctggatcc ggttttctgg ggatatgctc ccggatggtg ggataaaccg ccgctgatta
    30781 acgcccgcgt agaaactgcc tcaaccagtc gcatgtttaa accgctctgg caacatggtc
    30841 gggcaatctg ttttgccgat ggctggtttg agtggaaaaa agaaggcgac aaaaaaagcc
    30901 ttattttatc tatcgcgctg acggacaacc tgtttttatg gccgcgatag gtagcatacc
    30961 gtttgagcgt ggtgacgaag ccgaaggatt tttgatcgtc actgcggcgg cagatcaagg
    31021 tctggtagat attcatgacc gacgcccgct ggttctgtcg ccagaagctg cacgggaatg
    31081 gatgcggcaa gacattggtg gtaaagaagc ctcagaaatc gctaccagaa gctgtgtgcc
    31141 agcaaaccag ttcacctggc accctgtgtc gcgcacggtg ggtaatgtca aaaaccaggg
    31201 cgcggagtta attcaacctg tttgttgaca tcctccacgc cctgaatgac gaggacccct
    31261 gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gagcggcctg
    31321 actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagctc
    31381 catttacatc ggcgttcgct gtatatccac atacctggca tctgaattta ctttgtgaca
    31441 ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg tacgctggcg
    31501 gaacagcaag cacctgaccg ccacgccaga gctgcttgta ctcaagctgg cggcgcattt
    31561 cataccagcc ctgatccagt atcgaacggt ttaaacctga ttttgcccgg acattgcgcc
    31621 ccggctggct gaccgtaccc gctgctgact ttgacatgtt gctgaccttc aaatcctcaa
    31681 tgactatcat tacgtggttt ttgctgacgg tcgttgtgac tttgtgaagg tagtccctgc
    31741 ggatatttgc gatacaggaa tgcagtcgct gtattttgcg tttctgcttc tgccagttgt
    31801 tgctgaattt gaccttgcgg cttaactgtc gctgaagtct cgccagcttc ttctggtttt
    31861 tctgaaaact gtttacaggc tcaaagactg tgccatctga cagcgaggca agtttagcca
    31921 cgccagcatc cagcccgacc attgatgctg aagggtgaac cggagttgat acttcacttt
    31981 ctgcctgaat actgatgtac cacttaccgc aggactggct gacagtgaca tttttcacaa
    32041 cacccgtgac ctgacggctg ttccggtagc gcatccagcc aagttttggc agaaaaatac
    32101 ggctgttttc ctgagcgagc ttaacgccct gcggatagcg gaatgcatca ttctgtcacc
    32161 gctttttgaa tcaggggaac gccgagcgct tctggaaaaa gtttttgcag gcctgctcag
    32221 ggtctttcag tgactgttgc aatggctgtg acggagcatc tttaatccat tgcgtttcag
    32281 tggcattttt ccactcaacc aatcaggaag ccattttcgc gtaagggatg tctttgttac
    32341 cggccttatg attctcattc tgacgtgcca gagtacgact gaaaacgaaa cgacatgcgc
    32401 ctgcgaagcg cctcatttca cgcgcctgtt gaccaccggg tcttaaccgg aatttgaata
    32461 cctgtagtcg tttcacaggg caaattatac tttggtctgt gaaaaaaact gatatccgac
    32521 gcggaaggca ctgtgttttc ctgatgcatg ctcaccgggt ctttgttacc aagtaccgac
    32581 gccagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
    32641 cttattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catctgttaa
    32701 ttaactatcc ccccaaactg gcgatatcca gtctggtaaa caaacagcct caaaggcgta
    32761 tcgggtaggt tactgcgacg ggatcgacca gatattgctg ccaggtatta ccgcaaagat
    32821 gttttgtggc gtcccggcta ttttgccagt agctgcggag gtgcgccaat atccgtcatc
    32881 cgccaataca ttgaacaaca gcaaacacct ggtcagatga aaaaacgagc cttatatccc
    32941 cgccctgaag gacgggcttt tacggtgcac tggataagcc tggagattat ctacacctgc
    33001 atggacatca ttgtactatg ccccgtacat gccccatcac atcctcaaca tcaaagtatt
    33061 agagccttgc aaaccattat gcgtctcagt ttgtctcatc tatcttacgc aaaaaaaata
    33121 gaactaatat caagcggcgc tatattgcgt attaaataaa accacctcaa ttacattaat
    33181 aaatctaaat aaatatcatg caaataattt ccatggttat ttttaggcta tttaaattaa
    33241 aaaaataaat tatgagtcaa tcataaataa caacctttaa tataatttga ctaccaaatt
    33301 tatattcgtt cacaaaatag tcatgcattg tgtgcaatag aaacagttca gataaaaata
    33361 gggatctact tcacaattta aatgagaaac taaaacttac atcttgaaat aatctcattg
    33421 attggatgaa tatttgtagt gcagtgacat catttttaaa taatagttca aataaaaggg
    33481 ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt actgatggcg
    33541 atgtctgctc aggcagcaga gatttacaat aaagacagta acaagctgga tctgtacgga
    33601 aaagttaatg ctaagcacta cttctcttct aacgatgcag atgatggcga cacaacttat
    33661 gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc aactgactgg tttcggtcag
    33721 tgggaatatg aattcaaagg caaccgtgct gaatctcaag gttcttccaa agataaaacc
    33781 cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta tggccgtaac
    33841 tacggtgtag catacgatat cggtgcatgg accgacgttc tgccagaatt cggtggcgat
    33901 acctggaccc aaacagatgt gttcatgact ggtcgcacca caggtgttgc aacctatcgt
    33961 aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta tcagggtaaa
    34021 aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt tcggtttctc cactacttat
    34081 gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac taatgatcag
    34141 gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt atgggcagct
    34201 ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga aacccagaac
    34261 atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga agtagttgca
    34321 caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc taaaggaaaa
    34381 gacttgggtg cgtggggtga tcaggacctg attgaatata ttgatgtagg tgcaacctat
    34441 tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat tgataagagc
    34501 gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg attggtttac
    34561 cagttctaat ctgattactc aaagatatgt tgtgggaggc tttgcctccc caacatataa
    34621 gtggcgctct caagccactt tccttagaag cactatcttg cttctgacta tataaattac
    34681 cctttatttt ctgggacgtt tcaacgcccc atttttaata atactcagtg aacaattaac
    34741 gtatcaatta gatttaataa caacgatatc cgtacctaac ctgatatcta atgtcataag
    34801 catcccttta attatcccct caacctcttg taaatttccc actcttgagc tccacatagc
    34861 ccaaacgccg gatgcaaaaa aaatcacacg tgcatctctg aaattgtgat ggaggaagtg
    34921 agtatcaatt atatgtgctt ataaacgttc ccgattggca cagaagtcac cggattgttc
    34981 aggctccaat aggttgagta tgtcgtattt attttccaaa ataaaaatcg atgctgttgc
    35041 tccttaataa aatactttta ctctctggac gcttttctta gctctcttgg tgttatatta
    35101 aaacgattat ggaacctttc agtaaaacga gaaggacact tgtaaccatt ttccctggca
    35161 atctcgctga tgggttttac tgtcgtttgt atagcaggac aacgcattat ttaacctcac
    35221 gtcgtccaat atactttgaa aacttattcc ctcgcttgcc agacggcgat gcaatgtgga
    35281 aacagaaatg tagagatatc tcgctacctt gtttgctgtc cattttgttc taggttcgga
    35341 tagcattagg ttataacaac gacttattaa tgattgttta ctatctgata aaagtaaaca
    35401 attaacatga tgtgctccta atgaaagtag aacgcccatt gctaagtgtt cctgaatttg
    35461 cgttgagaag cctcgggaaa tagatgtttt tagttgctcc caacaatata ttaactcggg
    35521 gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga tcagttatcg cataaaattt
    35581 ttgaaacttt tcaattagat caatgggata ataaagcatt tctgcaagat aaagacctgc
    35641 ttctggataa ttcacaatat aaaattcata tccacaggga aataatatta tctgattatt
    35701 atcaacagtt agactatgcg tctcccaatt gataactttt tttccctgac ggatacgaca
    35761 taaagctggc ataagaggct taaccctgtg aatctcatga tgtttatgca tccgtatttc
    35821 ttcaatcttt aggttagact tatctcttgc cagcatactc ttaccctatt ttatctcata
    35881 aactggtgtt atctcagcgg ttgcaatttt attggcatta agcatataac caactaacgc
    35941 tccgctggaa ttagaatcca caggaatctt atcagttttt agagcccata ctttaaactg
    36001 gtaatgatgg ggtttatctc ctttgggagg acatgctcca ccaaatcctg cataaccaaa
    36061 atcatttcgg ccttgaacag caccagtcgg aagttttgta ccatcacgtc ttcctgcatc
    36121 agtgggcaaa tatgttacag ttgctggaat attagcaaca gtccagtgcc accatccact
    36181 gcctgtagga gcatctggat catacacagt tacggcaaag cttttggtgc cttcaggagc
    36241 accagaccag gttaatgagg gcgatgtatt accaccttca cacccaaatc cagaaaatac
    36301 atgagacgtt gtaagttgct ctcctgtttt tatttcatta ctagtgacct gaaatgctgc
    36361 ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa acgataagtt ttttcataaa
    36421 aacctcattg ttgtgaccta tcgttatttt atttgatgtg ccttgatctc attatgaata
    36481 aaggcgcaat gatcgcgcaa atgcaatcat aattataaag gtgccccgaa cattagccat
    36541 aatatgcaca aatcagattc ctgttatctc aaggagaatt attttttaat gcggtgcact
    36601 tcgtgacaac aagttaatga ctaacacacc ggcacaaatc aacatcatgc ctatgatagc
    36661 tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc aaactaatca gaacgatacc
    36721 gactcctgac cagatagcat aagcaatccc tgtaggtata taagccagcg tctgagctaa
    36781 taaccagaat gatgcacaat agcaaataat tgtgccaaca gagggccata accgtgtaaa
    36841 cccctctgaa aacttcatta aggttgtacc aatgacctct gcaagtattg cgccaccaag
    36901 ataaatataa gggttcatag catattcttt cctgttcata ctggagagaa ttgtactaca
    36961 gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt atccaagggg ctctggcgtg
    37021 gcaagagata aaggcaggaa caataatgat cagaaggaac cccaggctaa cgaatgagtt
    37081 cactcttcct gggagcaaag cgaagacaaa acatgttatc gcgtttacgg atcgtggatg
    37141 gcagaaaaca accaggatca ggtatccatt ttaaaccaga aattgagtga gtttgcccca
    37201 tccatgcccc acgctgtggg atcggatata attaaacaag catagtcaac aggttacaca
    37261 acctacacct gcatggacat cacttcctga tacgccgcca ccagcttatt acgcacctga
    37321 atccccattt gcatagaaac tgaggctttt tgcatatcgg tcatcacatc gtttagcgcc
    37381 acgccgggtt caccgagggt gaatttttct gcctgcgtgc gggcgactgt ttgtgtatcg
    37441 cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac taatggtcgg ttgcggcagt
    37501 gattcctgcg cacacgcact catcgccgta gcctgtaact ggctgataac cccttcaatc
    37561 ccctgtatcg ctgacatttt cgtctcccgg ataatttctg gtaacaaagc ctaccagtaa
    37621 gtcaataaga caaaggcgct aaatagcaac aaaaaaactg gtttattggc ggatagaaaa
    37681 aacgaaagca caaataatgg gagcgtcaat ttttcgagtt tgctgacccg agagtgagtc
    37741 ttgttccact ttgccaataa cgccgtccat aatcagccac gaggtgcgcg atgaatgcga
    37801 ctgcagccca gacaaaatct cttgagtggc ttaatcgcct gcgtgcgaat ccgaaaattc
    37861 cattgattgt taccggttcc gcggcagtgg cggtcatggt cgcactgatc ctgtgggcga
    37921 aagcccccga ctaccgcaca ttattcagca atctttccga tcaggatggt ggcgcaattg
    37981 tcagccaact gacgcaaatg aatattcctt accgcttcag cgaagccagc ggcgctattg
    38041 aggttccggc agataaagtt cacgaactgc gcctgcgcct ggcgcaacaa ggtttgccca
    38101 aaggcggcgc ggtcggtttc gaactgctcg atcaggaaaa gtttggtatc agccagttca
    38161 gcgaacaggt gaattatcag cgggcgctgg aaggcgagct ttctcgtacc atcgaaacta
    38221 tcggcccggt aaaaggggcg cgcgtacatc tggcaatgcc gaaaccgtct ttattcgtcc
    38281 gtgaacaaaa atccccttct gcatcggtga cggtaaatct gttacccggc cgcgcactcg
    38341 atgaagggca aattagcgct attgtacatc tggtttccag cgcggttgct ggtctgccgc
    38401 ctggaaacgt cacgctggtg gatcagggcg ggcatctgtt aacccagtcc aataccagcg
    38461 ggcgcgatct taatgacgct cagttgaaat atgccagcga tgtcgaaggc cgtattcagc
    38521 agcgtattga agcgatcctg tcacctattg ttggtaacgg taatattcac gcctaggtca
    38581 cggcgcaact ggacttcgcc agtaaagaac aaacggaaga acagtatcgc cctaacggtg
    38641 atgaatctca tgcggcgctt cgttcacgcc agcttaatga gagcgagcaa agcggttccg
    38701 gttatccggg cggcgtaccg ggggcgttgt cgaatcaacc ggcacctgcg aataacgcgc
    38761 caatcagcac gcctccgaca aatcaaaata accgccagca gcaggcgagc accaccagca
    38821 atagtgggcc gcgtagcaca cagcggaatg aaaccagtaa ctacgaagtc gatcgcacca
    38881 ttcgtcatac caaaatgaac gtgggcgatg tgcaacgtct gtcagtcgcg gtagtggtga
    38941 attacaaaac cttgccagac ggtaaaccat tgcctctcag caacgaacag atgaagcaaa
    39001 ttgaagctct gacccgcgag gcgatgggct tttctgaaaa acgcggtgat tcgctcaatg
    39061 ttgttaactc gccgttcaat agcagtgacg aaagcggcgg agcactgccg ttttggcaac
    39121 agcaagtgtt tatcgatcag ttgctggctg ccggtcgctg gttgctggtt gctggtactg
    39181 ctggtggcgt ggttgctgtg gcggaaagcg gtacgcccgc agctaacacg tcgcgccgag
    39241 gcggtgaaga ctgtgcagca acaggcgcag gcccacgagg aagtggaaga tgcggtggaa
    39301 gtccgtctga gcaaagacga acaactccaa caacggcgcg ctaaccaacg actgggggca
    39361 gaagtcatga gccagcgtat ccgtgaaatg tctgataacg atccgcgcgt ggtggcgctg
    39421 gtcattcgcc agtggataaa taacgatcat gagtaacctg acaggcaccg ataaaagcgt
    39481 catcctgctg atgaccattg gcgaagaccg ggcggcagag gtgttcaagc acctctccca
    39541 gcgcgaagtg caaaccctga gcgctgcaat ggcgaacgtc acgcagatct ccaacaagca
    39601 gctaaccgat gtgctggcgg agtttgagca agaagctgaa cagtttgctg cactgaatat
    39661 caacgccaac gattatctgc gttcggtatt ggtcaaagct ctgggtgaag aacgtgccgc
    39721 cagcctgctg gaagatattc tcgaaacccg cgataccgcc agcggtattg aaacgctcaa
    39781 ctttatggag ccgcagagcg ccgccgatct gattcgcgat gagcatccgc aaattatcgc
    39841 caccattctg gtgcatctga agcgcgccca ggccgccgat attctggcgt tgttcgatga
    39901 acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt ggcggcgtgc agccagccgc
    39961 gctggccgaa ctgaccgaag tactgaatgg cttgctcgac ggtcagaatc tcaagcgcag
    40021 caaaatgggc ggcgtgagaa cggcagccga aattatcaac ctgatgaaaa cgcagcagga
    40081 agaagccgtt attaccgccg tgcgtgaatt cgacggcgag ctggcgcaga aaatcatcga
    40141 cgagatgttc ctgttcgaga atctggtgga tgtcgatgat cgcagcattc agcgtctgtt
    40201 gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa ggggccgagc agccactgcg
    40261 cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat attctgcgcg acgatctcgc
    40321 caaccgtggt ccggtgcgtc tgtcgcaggt cgaaaacgaa cagaaagcga ttctgctgat
    40381 tgtgcgccgt cttgccgaaa ctggcgagat ggtgattggc agcggcgagg atacctatgt
    40441 ctgataatct gccgtggaaa acctggacgc cggacgatct cgcgccacca ccggcagagt
    40501 ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga ggccgaaccc agccttgagc
    40561 agcaactggc gcaactgcaa atgcaggccc acgagcaggg ttatcaggcg gggattgccg
    40621 aaggtcgcca gcaaggtcac gaacagggct atcaggaagg actggcccag gggctggaac
    40681 aaggtctggc agaggcgaag gctcaacaag cgccaattca tgcccggatg cagcaactgg
    40741 tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt gatagcttcg cgcctgatgc
    40801 agatggcgct ggaggcagca cgtcaggtga tcggtcagac accaacggtg gataactcgg
    40861 cactgatcaa acagatccaa cagttgttgc agcaagaacc gttattcagc gggaaaccac
    40921 agctgcgcgt acacccggat gatctgcaac gtgtggatga tatgctcggc gcaactttaa
    40981 gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca ccctggcgga tgtaaagtct
    41041 ccgccgatga aggcgatctc gacgccagtg tcgccactcg ctggcaagaa ctctgccgtc
    41101 tggcagcacc aggagtggtg taatgaccac gcgcctgact cgctggctaa ccacgctgga
    41161 taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt cgctacgggc gattaacccg
    41221 cgctaccggg ctggtgctgg aagccaccgg attacaattg ccgctcggcg caacctgtgt
    41281 cattgagcgc cagaacggca gcgaaacgca cgaagtagaa agcgaagtcg ttggctttaa
    41341 cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa ggtgtcctgc ccggcgcgcg
    41401 tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc ggcaagcagt tgccgctcgg
    41461 tccggcgtta ttaggtcgtg ttctggacgg tagcggtaaa ccgctcgatg gcctgccctt
    41521 ccccgatacg acggaaaccg gtgcgctgat taccccgcca tttaacccgt tgcaacgtac
    41581 accgattgaa catgtgctgg acaccggcgt gcgcccaatc aatgccctgc ttaccgtcgg
    41641 acgcgggcag cgtatggggc tgtttgccgg gtcgggcgtt ggtaaaagtg tgctgctggg
    41701 gatgatggca cgctacaccc gcgccgatgt cattgtcgtg ggtttgattg gtgaacgtgg
    41761 gcgcgaagta aaagatttta ttgagaatat cctcggtgcc gaaggacgtg cacgctcagt
    41821 ggtgattgcc gctccggcgg atgtttctcc gctcctgcga atgcaaggtg ccgcctatgc
    41881 cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat gtgttgctga ttatggactc
    41941 cctcacccgc tacgcgatgg cccagcgtga aattgcgctg gcgattggcg aaccacctgc
    42001 cactaaaggt tatccaccgt cggtgtttgc caaattaccg gcactggtcg agcgtgccgg
    42061 taatggcatt agcggcggcg gctcgattac cgcgttttat accgtgctca ccgaaggcga
    42121 tgaccagcag gaccccattg ctgactccgc gcgggcaatc ctcgacggtc acattgtgct
    42181 gtctcgccga ctggcggaag ccgggcacta tccggctatc gatattgaag cgtcgatcag
    42241 tcgcgcaatg acggcgttga tcagtgagca acattacgcg cgagtgcgca ccttcaaaca
    42301 gctgttgtcg agttttcagc gtaaccgcga tctggttagc gtcggcgcgt atgccaaagg
    42361 cagcgatccg atgctcgata aagccatcgc cctgtggccg cagctggagg gctatttgca
    42421 acaaggcatt tttgaacgcg cggactggga agcgtctctc caggggctgg agcgtatttt
    42481 cccgacagtg tcataaccca ggagataacg gcagatggcg gaagaacagc tcaaaatgtt
    42541 gatcgattat cagaatgaat atcgcaataa cctcaatagc gatatgagtg ccgggatgac
    42601 cagcaaccgc tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca
    42661 gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg
    42721 agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc
    42781 actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc
    42841 catgaggaaa cctgaatgat tcgcttagcg cccttgatta ccgccgacgt tgacaccacc
    42901 acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctcacgtt gttgagcgaa
    42961 gcattagcag gcgagacaac aactgacaaa gcggcccccc agttgctggt agcaacagat
    43021 aagcccacga caaaaggcga gccgctggtc agcgatattc ttgccgatgc gcaacaagcg
    43081 gatttactga tccctgtgga tgaaacactt cctgtcatca acgacgaaca atccacatca
    43141 acaccgttaa ccaccgctca gacgatgacg ttggctgcgg tggctgacaa aaatacgaca
    43201 aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg caagtctgag cgcccttttt
    43261 gcgatgttgc cgggttttga caatacgccc aaagtgactg atgcaccgtc aaccgtgtta
    43321 ccggcagaga aaccaacgct cttcacaaaa ctgacttctg cgcaactcac tacagcacag
    43381 cctgatgacg cccccggcac accagctcag ccattaacac cgctggtagc agaagcccag
    43441 agtaaagcgg aagtcatcag cacgccttcg ccggtgaccg ctgacgccag cccgctaatc
    43501 actccacacc agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg
    43561 ggttctcacg aatggcaaca atcattgagc cagcatattt cgctgttcac ccgccagggg
    43621 caacaaagtg cagagttgcg tctgcaccca caggatttag gtgaagtgca aatttccctc
    43681 aaagtggatg ataaccaggc gcagatccag atgatttcac cgcatcagca cgtacgcgcc
    43741 gccctggaag cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta
    43801 gggcaaagca acattagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa
    43861 cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg gggaagacga cgatacgctt
    43921 ccggttcccg tctctttaca agggcgtgta acaggtaaca gcggcgttga tattttcgcc
    43981 taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc
    44041 aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg
    44101 cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg
    44161 cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca
    44221 aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc gctggatacc tttacggtca
    44281 atttgggcga tgcggatcgc gtgctttata tcggcataac cctgcgactg aaagatgaag
    44341 caacccgctc gcggctgagt gagtatttgc cggaagtccg tagtcgcttg ctgttactgt
    44401 tttcacgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg
    44461 agattaaaac cacactttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg
    44521 acgtgctgta taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa
    44581 gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt
    44641 gttagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc
    44701 gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg
    44761 ctgttcaacc tgctgcgccg tagcccggac attaccgtcg gggccatccg cattcagccg
    44821 taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa
    44881 ccgctacgcg gcacagggct ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat
    44941 aacctgtttg gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat
    45001 actgaacagc gcgtcatcaa ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc
    45061 tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtggaattt
    45121 accaatatca ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt
    45181 ggcaacctga ccggcgaatt taatatctgc ctgccattca gcatgatcga gccgctacgg
    45241 gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat
    45301 aacctggtgc gccaggtgca gcattcacag ctggaactgg tggccaactt tgccgatatc
    45361 tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa
    45421 cccgatcgca tcatcgccca tgttgacggc gtcccggttc tgaccagtca gtacggtact
    45481 ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg
    45541 aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa
    45601 tggacgatct gtgggctgaa gcgttgagcg agcaaaaatc aaccagcgaa aaaagcgctg
    45661 ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg
    45721 acctgattat ggatattccg gtcaagctga ccgtcgagct gggccgtacg cggatgacca
    45781 tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc gctggacggt ctggcgggcg
    45841 aaccgctgga tattctgatc aacggttatt taatcgccca gggcgaagtg gtggttgttg
    45901 ccgataaata tggcgtgcgg atcaccgata tcattacccc gtccgagcgg atgcgccgcc
    45961 tgagccgtta gtgatgaata accatgctac tgtgcaatct tccgcgccgg tttctgctga
    46021 gccactgctg caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc
    46081 ctggctggta aaacggctgg gatttgcccc taaacgcact ggcgttaacg gtctgaaaat
    46141 tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg gtggtcgatg tggaagatgc
    46201 acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc
    46261 agcaccaacg gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa
    46321 gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg caccggtcct tctctggctg
    46381 attacgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct acctggcggt
    46441 ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt gacgttcatt
    46501 ccagcgattt tactgatgat gaccagtttc acccgcatca ttattgtttt tggtttattg
    46561 cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg tattgctggg gctggcactg
    46621 tttttgacct tttttattat gtcgccagtg atcaacaaaa tttatgtcga tgcgtaccag
    46681 ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg aaaaaggggc gcagccgctg
    46741 cgtgagttta tgctgcgtca gacccgcgag gcagatttag ggttgtttgc cagactggcg
    46801 aataccggcc cgttgcaggg gcctgaagct gtgccgatgc gcattttgct cccggcctac
    46861 gtgaccagcg agttgaaaac cgcatttcag ataggcttca cgattttcat cccttttttg
    46921 attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggttcccccc
    46981 gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg
    47041 ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac acctgaatcg
    47101 gtcatgatga tgggaactga agcgatgaaa gtcgcgctgg cactggcagc cccgctgttg
    47161 ctggtagcgc tggtcacggg ccttatcatc agtattttgc aggcagccac gcagattaac
    47221 gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat ttatcgccat cattattgcc
    47281 ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg
    47341 tatatcatcg ggtagcccgt acaatgatgc aggagacaag cgatcaatgg ctttcctggt
    47401 taagcctgta cttctggccg ttactgcgcg tgctggcgct catttctacc gcgccaattc
    47461 tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg atcacgttcg
    47521 ccattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc
    47581 tggccgtaca gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg
    47641 ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca tttgcgacat
    47701 ttgtcgatcc tggcagccat ctcaatatgc ccgttttagc gcgcatcatg gatatgctgg
    47761 cattactgct gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata
    47821 cctttcacac cctgccgatt ggtagcgaac cgttgaacag caatgcgttt ctggcaccca
    47881 ccaaagcagg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc
    47941 tgttgctgac cctgaatctg gcattaggtt tactgaatcg tatggccccg caattatcca
    48001 tttttgttat tggatttcca ttaactctga ctgtcggcat ctctttaatg gcggcattaa
    48061 tgccgttaat tgcgcctttt tgcgaacatt tattcagtga aatttttaat ttgctggctg
    48121 atattattag tgaattacca ttaatataat tccgtaacgt ttatcatgct accctaagga
    48181 ttatccgaaa aataatatct gcgaacatct tccaggatac tcctgtagcg aaatatttgt
    48241 tttaagctca ctcacatatc gcaacattta ctttaagaca attccaggca aattatacaa
    48301 cactttacgg gatagtaagt ccgcctgaaa aattgcgaga gtggcgcatt aggtgaccca
    48361 tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga
    48421 gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt
    48481 aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt
    48541 tgatgacctt gccatagctt gtgattcaca gcgtccttca gtggtgttta ttaatgagga
    48601 ctgtttcatc cacgatgctt ctaacagtca gcgcatcaag cacatcatta atcaacatcc
    48661 caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt
    48721 ggtcagaaaa aatttattga tcagttctaa atcgattaaa ccggaatctc tcgacgatat
    48781 ccttggcaat attctgaaaa aagagacaac gataacctcg tttttaaata tgccgacgtt
    48841 atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat
    48901 tcaaatctcc gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat
    48961 taaacgtaag atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga
    49021 taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg gtgatttccc
    49081 accagtcagg ctgaataaga ttactctgct ttctcaacaa agataccgtc ctgatgccct
    49141 gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgcaaccag gtacattgtg
    49201 ccttcactaa actcctcaac tgccagtacc actccaggac gacgcggacc gccatccgtt
    49261 ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta
    49321 gaacaatttc gtttttctgg cagcgccaag cgcgcgcgag tgctgatttt ctcgacggtc
    49381 tatacttaag agatgctagg cggacttaac gactggcggc aacaacagag taacggttgc
    49441 gaggaaagat gatgaaaacc gcaaaagtgt acagcgatac cgcaaaacgt gaggtcagcg
    49501 tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca
    49561 gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat
    49621 tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa
    49681 ttcacaataa aaaaatcccg accctgaggg tgtcgggatg aaacttgctt aagcaagaag
    49741 cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat ctgcaatttt
    49801 tttcgctgac aagaaaatat tcgcgctatg aatgattaac gctacttatg aatagaaatg
    49861 tgacgcgcgt caccccttgg tggctctggt gtcaacggga tcgctcccga aaaaatcatc
    49921 tttgattatt tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcgaccatgt
    49981 tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc
    50041 atagttatga ctggcaaccg gcagccccct ggctcagccg tttacacgaa gccaatattc
    50101 ccgtcattct ctgtagcagt aaaactttag cggaaatgct gtacttgcaa aaaatgttgg
    50161 ggctacaagg cttaccgctg attgcagaga atggcgcagt gatccagctt gctgaacaat
    50221 ggcaggatat agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatctgcc
    50281 aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttt gatgatgtcg
    50341 acgatgcaac cattgccgaa tggacgggat taagccgtag ctaggcggcg ctgacgcagc
    50401 tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta
    50461 tcgctcgtct gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtac
    50521 tggatgcctc tgccggaaaa gatcaggctg ccaattggat tatcgcgacc tatcaacaat
    50581 tgtcaggcag acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcctttac
    50641 tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtacatctgc
    50701 atgatgagga tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg
    50761 aagggctgga ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattgc
    50821 gcccggcctg ttttgcgagg taaagccgac ggtcggccaa tgactggagt tgttcaaaat
    50881 cataatcacc ggtttcctcg ctgctactta cccccagcga ggcactgatg cgtatcgttg
    50941 tactcttggc gatcagcatc tctttttcat ttaacttcag gcgaatacgt tctgcgacct
    51001 ccgcagcctg cgtcagattc gcgcctggca gaatcacaca aaactcctca ccaccgaccc
    51061 gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa
    51121 cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga
    51181 cctgaatgac agaaaaagga tgttggtgcg tctgacacaa tttagcgagc ggacgggctt
    51241 tttcgaacag tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact
    51301 gcaacgagct ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga
    51361 ggagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga
    51421 aatcgccgcg cacgccttcg cttagcgtat ggacgcgcgc cagcacaccg tcgaaatggt
    51481 ccagacgttc ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt
    51541 ccattgcctg cgccagcaag gccagttcac gaggatcaaa aatattccct gttggatggt
    51601 caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga
    51661 ggtttttatc gatggcgttt cttaaaaatt gctgcatagt acgcacggga atactcatcc
    51721 ccagcacgcc ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt
    51781 cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt
    51841 ctcgttgcga atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggta
    51901 cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccagcacgc gagacataca
    51961 tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca
    52021 gtgcagcggt aatttcattg tcgaggcttt cattttcgcg agacaggaaa ttcccctcgc
    52081 tgactaatgc atccgatacg ccattgactg gcagggtgcg ccgtcggttg agttcgattt
    52141 gccaggcgtg ctcgtcgcga tgctgttcga actcggttac ggcattacgc agagaggtga
    52201 aatccagtgg cgccacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga
    52261 cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctagc
    52321 tggagatata ggcatcttcc agcaccacca cttcacgcca ggtgagcaag gtggaaaaaa
    52381 gcaataccac gataaagcag agattaacga catgaccggg ccccaggcgg cgtgcgagtt
    52441 ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc
    52501 tgctttttta tgattctggc ataacccggc aatagcgtac cgcaaaacag cgataatttt
    52561 ataatggtgg catgataaga cgcgactatc attcattgca ggcattggga caccaggccg
    52621 gatgtgatcc ggcctggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc
    52681 ctcccccgga acaagatcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata
    52741 ccaacgacac tcaaccatac cgctggagta tccagtgaca atcatccgcg ggccgccctc
    52801 tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa
    52861 acttgttcac cttagttgaa gatggcgaat tttgcataat agccagtgcg agtattaatg
    52921 tgcctgaccg cgcattttcg ctactgcttt cactcccagc accacgatgc cgccgatgat
    52981 aaatccaaga atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg
    53041 cccggcgata tgttcaatag cgtgatgcag cggcgcaata ccatgtacca caatcccgcc
    53101 gccgacgagg aacatcgcca gcgtgccgac aatcgataat gctttcatca gccaaggcgc
    53161 gataatcaat aaccctttac ctaatgcctg catcagcgcg ctagattttt ccgccagcca
    53221 ataccctagg tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac
    53281 taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgcct ctgccacaat
    53341 ccccagcgtg atggcgacga tttccgcaga caaaataaaa tcggtacgaa tcgccccttt
    53401 aattttgtcc ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg
    53461 gctctgcgct ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac
    53521 cccttcaaag cagagaaacg cgccaccaat catcaacagt ggcgtaatcg cccacgggat
    53581 aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattgatca gcgatccttt
    53641 cgccacgccc cagaccacgg gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc
    53701 attgagcgat aaatcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat
    53761 cacagagata tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc
    53821 ggccaaaagc attccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg
    53881 cagctctgat acttgcttca caggtcaaca tttatttaac aatcaagtgc aattatatct
    53941 ctcgccagca gaatgatttt cacgtttact atgaaccacc tttttatttc cgccgtgagg
    54001 gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat
    54061 ttggggctca acctattttg tcattcggat tggcgtggga agctggcctc cgttaatgat
    54121 ggcgggcgtt cgattccagg cagccggtat tttattgctg gcatttttgc tactgcgcgg
    54181 acacaaactt cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct
    54241 ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc
    54301 cgccgtagtg attgcaaccg tgcccctctt taccctgtgc ttcagtcgcc tgtttggcat
    54361 taaaacgcgc aaactggaat gggtgggtat tgccattggg cttgccggaa tcatcatgct
    54421 caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc
    54481 gattagctgg gcgtttggct cagtttatgg ctcgcgcatt accttacctg tagggatgat
    54541 ggcgggtgcg attgagatgc tggcggcagg cgtggtgtta atgatcgcat cgatgattgc
    54601 gggtgaaaaa ctgacggcgc tcccttccct gtcaggcttc cttgcggtcg gctatctggc
    54661 gctgtttggt tcgattatcg ccattaacgc ttatatgtat ttaatccgta atgtcagtcc
    54721 ggctctcgcc accagctacg cctacgttaa cccggtggtc gcggtcttgc tgggtacggg
    54781 actgggtggc gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgttttcgc
    54841 ggtggtactg gtcacgttgg gaaaatatct cttcccggcc aaacccgttg tcgcgccagt
    54901 tattcaggat gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac
    54961 cttcgccgca gatccactct tccagacgct cggtaagcgc cgcatccgtc agcttctcgc
    55021 gcctacgtaa cgcacactcc cagacaatca gtacgcgcca gccgagctcc tgcaagcgac
    55081 tgatatcgcg gcgatcgcgt tcaacatttt tacctatctt ctccagccag aactcagttc
    55141 gagtcgcagg cactttaaac agatagcagt gatgatgatg ccagaagcag ccgtgggtaa
    55201 atatcacgca gcgatattca tcaacgacaa aatccgggcg tccgggcaga ctggcgtcct
    55261 gaacgcgaaa cgcaaggccc tgcccggtta acaaactggc gaggcgcttc tcaatcgccg
    55321 tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga
    55381 ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt
    55441 tttgccaccg cggcaaagac cggcacgacc accgagttgc cgaactggcg ataggcctga
    55501 gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc
    55561 gcgcattcac gaggcgttaa ccgacgtgga cgatgttgct gattcagcgg atcgtcaaag
    55621 tctttctcac ccgtggccat atcccaaccg cgatcgatta aaatttccgc gccatctttg
    55681 taataacgag cagacagcgt acgggtgacg ctttgcggat tgttcggata aaccattcca
    55741 taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac
    55801 agcaccggcg tcaggatata tttcgcctca accatcgggt ccagcagctg cgccagcgtc
    55861 actcgctgtg cagggaaaca ttcgctgata tcacgcagag taaaatcggc tttcagattc
    55921 agatcgcgac gaaaacccac cagcacgatg cgttcacggt gctgcggcag gaaatgttta
    55981 ccatcgatga ttttcggatc gtccggcccg ttatcttctg catcggcaac gtcatagccc
    56041 agttcgtcca gcgtctgcat gatgatgcgg aacgtttttc ccttatcgtg actcttcagg
    56101 tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca
    56161 tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc
    56221 tttttcgata cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg
    56281 tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttga
    56341 tgactgaggg tgatgtcgcg gatatcttca ttaaaatgat gcgtcgccgg gtcgcaataa
    56401 tggttggctt tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcactgt
    56461 ccaccaatcg attcaaaacc acgacggatg ccgccaattc cggcaaatag atcgataaag
    56521 cgaaacgcat aatgcggatg atgttccggt ggtttgggta acaacgtttg cagatgggca
    56581 aactctttct cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt
    56641 gccgcgctcc agtgattctc acccacacca ttaagctgcg ccaccagcat tttaacatca
    56701 taaatttgca gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg
    56761 gttactgata tattttcctg catagattta accggccatc tgagatggcc gacaggttaa
    56821 cataattact gccgtttagg gccacgcgct cagccagcgg gtgaaaacgc atctatcacc
    56881 ttgtgatcga taccttcatt ctcccccgcc agttcggcac cgagcttcgc catcaaacag
    56941 cgccaagctc aatgcccctg aaacggcaaa tacgcgcgcc aggccaatcg cccccagggc
    57001 ttccagccga tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc
    57061 gctgaaactg tgagcggcaa tggcatgaca aacggcctca attttctccg ccggaaattg
    57121 cacgaactct tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg
    57181 ccgttgcgga tgatttttcg ccaggctcac gatatcgtga aaataacagg cggttaaaat
    57241 caccagcata tcgacatcgt catctgccgc cagtttctgt gccgtagccc agacacggcg
    57301 aaaatgacac aggtcatgcg ccgcgtcctg atgttgatga tggtttttca accagttctc
    57361 gaactgtgcc tgccagtgtt gtaactccat acgcgctccg ttgccaaaga gtcgcaacct
    57421 tagcagtttt tattcatagc gtctgcaacg tgcgggacga ggtcgggtgt accatgcaat
    57481 gccgcctaac aaggcaccaa ccagggcc
//
LOCUS       DAJTUN010000008        56359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTUN010000008 DAJTUN010000000
VERSION     DAJTUN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.008"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..477
                     /gene="yeiP"
                     /locus_tag="OO470_000479"
     CDS             <1..477
                     /gene="yeiP"
                     /locus_tag="OO470_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6249840.1"
                     /translation="RGAATLYKMRFSDVRTGLKVEERFKGDDIVDTVTLTRRYVDFSY
                     VDGNEYVFMDKEDYTPYTFTKDQIEEELLFMPEGGMPDMQVLTWDGQLLALELPQTVD
                     LEIVETAPGIKGASASARNKPATLSTGLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            700..2166
                     /locus_tag="OO470_000480"
     CDS             700..2166
                     /locus_tag="OO470_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6249841.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2284..3270
                     /gene="yeiR"
                     /locus_tag="OO470_000481"
     CDS             2284..3270
                     /gene="yeiR"
                     /locus_tag="OO470_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6249842.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3309..4022
                     /gene="lpxT"
                     /locus_tag="OO470_000482"
     CDS             3309..4022
                     /gene="lpxT"
                     /locus_tag="OO470_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6249843.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4434..5000
                     /gene="mepS"
                     /locus_tag="OO470_000483"
     CDS             4434..5000
                     /gene="mepS"
                     /locus_tag="OO470_000483"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6249844.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5181..6737
                     /locus_tag="OO470_000484"
     CDS             5181..6737
                     /locus_tag="OO470_000484"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6249845.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            6819..8633
                     /locus_tag="OO470_000485"
     CDS             6819..8633
                     /locus_tag="OO470_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6249846.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8634..9728
                     /locus_tag="OO470_000486"
     CDS             8634..9728
                     /locus_tag="OO470_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6249847.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9728..10753
                     /locus_tag="OO470_000487"
     CDS             9728..10753
                     /locus_tag="OO470_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6249848.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10755..12344
                     /gene="yejF"
                     /locus_tag="OO470_000488"
     CDS             10755..12344
                     /gene="yejF"
                     /locus_tag="OO470_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6249849.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12348..12692)
                     /locus_tag="OO470_000489"
     CDS             complement(12348..12692)
                     /locus_tag="OO470_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249850.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13025..14215)
                     /gene="bcr"
                     /locus_tag="OO470_000490"
     CDS             complement(13025..14215)
                     /gene="bcr"
                     /locus_tag="OO470_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6249851.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14243..14938)
                     /gene="rsuA"
                     /locus_tag="OO470_000491"
     CDS             complement(14243..14938)
                     /gene="rsuA"
                     /locus_tag="OO470_000491"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6249852.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15088..16848
                     /locus_tag="OO470_000492"
     CDS             15088..16848
                     /locus_tag="OO470_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6249853.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            16973..17257
                     /gene="rplY"
                     /locus_tag="OO470_000493"
     CDS             16973..17257
                     /gene="rplY"
                     /locus_tag="OO470_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6249854.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17396..18403)
                     /gene="yejK"
                     /locus_tag="OO470_000494"
     CDS             complement(17396..18403)
                     /gene="yejK"
                     /locus_tag="OO470_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6249855.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18585..18812
                     /locus_tag="OO470_000495"
     CDS             18585..18812
                     /locus_tag="OO470_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6249856.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            18832..20592
                     /gene="pbgA"
                     /locus_tag="OO470_000496"
     CDS             18832..20592
                     /gene="pbgA"
                     /locus_tag="OO470_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6249857.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20667..20743
                     /locus_tag="OO470_000497"
     tRNA            20667..20743
                     /locus_tag="OO470_000497"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20701..20703,aa:Pro,seq:ggg)
     gene            complement(20846..23437)
                     /gene="yejO"
                     /locus_tag="OO470_000498"
     CDS             complement(20846..23437)
                     /gene="yejO"
                     /locus_tag="OO470_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6249858.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEINGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            23757..24404
                     /gene="narP"
                     /locus_tag="OO470_000499"
     CDS             23757..24404
                     /gene="narP"
                     /locus_tag="OO470_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6249859.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24615..25667)
                     /gene="ccmH"
                     /locus_tag="OO470_000500"
     CDS             complement(24615..25667)
                     /gene="ccmH"
                     /locus_tag="OO470_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6249860.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25664..26221)
                     /gene="dsbE"
                     /locus_tag="OO470_000501"
     CDS             complement(25664..26221)
                     /gene="dsbE"
                     /locus_tag="OO470_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6249861.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26218..28161)
                     /gene="ccmF"
                     /locus_tag="OO470_000502"
     CDS             complement(26218..28161)
                     /gene="ccmF"
                     /locus_tag="OO470_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6249862.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28158..28637)
                     /gene="ccmE"
                     /locus_tag="OO470_000503"
     CDS             complement(28158..28637)
                     /gene="ccmE"
                     /locus_tag="OO470_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6249863.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28634..28843)
                     /gene="ccmD"
                     /locus_tag="OO470_000504"
     CDS             complement(28634..28843)
                     /gene="ccmD"
                     /locus_tag="OO470_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6249864.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(28840..29577)
                     /gene="ccmC"
                     /locus_tag="OO470_000505"
     CDS             complement(28840..29577)
                     /gene="ccmC"
                     /locus_tag="OO470_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6249865.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29619..30281)
                     /gene="ccmB"
                     /locus_tag="OO470_000506"
     CDS             complement(29619..30281)
                     /gene="ccmB"
                     /locus_tag="OO470_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6249866.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30278..30895)
                     /gene="ccmA"
                     /locus_tag="OO470_000507"
     CDS             complement(30278..30895)
                     /gene="ccmA"
                     /locus_tag="OO470_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6249867.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(30914..31516)
                     /gene="napC"
                     /locus_tag="OO470_000508"
     CDS             complement(30914..31516)
                     /gene="napC"
                     /locus_tag="OO470_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6249868.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31526..31975)
                     /gene="napB"
                     /locus_tag="OO470_000509"
     CDS             complement(31526..31975)
                     /gene="napB"
                     /locus_tag="OO470_000509"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6249869.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(31972..32835)
                     /gene="napH"
                     /locus_tag="OO470_000510"
     CDS             complement(31972..32835)
                     /gene="napH"
                     /locus_tag="OO470_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6249870.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(32822..33517)
                     /gene="napG"
                     /locus_tag="OO470_000511"
     CDS             complement(32822..33517)
                     /gene="napG"
                     /locus_tag="OO470_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6249871.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33524..36010)
                     /gene="napA"
                     /locus_tag="OO470_000512"
     CDS             complement(33524..36010)
                     /gene="napA"
                     /locus_tag="OO470_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6249872.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36007..36270)
                     /gene="napD"
                     /locus_tag="OO470_000513"
     CDS             complement(36007..36270)
                     /gene="napD"
                     /locus_tag="OO470_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6249873.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36260..36754)
                     /gene="napF"
                     /locus_tag="OO470_000514"
     CDS             complement(36260..36754)
                     /gene="napF"
                     /locus_tag="OO470_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6249874.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            36863..37027
                     /locus_tag="OO470_000515"
     CDS             36863..37027
                     /locus_tag="OO470_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249875.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37162..37650
                     /gene="eco"
                     /locus_tag="OO470_000516"
     CDS             37162..37650
                     /gene="eco"
                     /locus_tag="OO470_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6249876.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(37800..38819)
                     /locus_tag="OO470_000517"
     CDS             complement(37800..38819)
                     /locus_tag="OO470_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6249877.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39053..39412)
                     /locus_tag="OO470_000518"
                     /pseudo
     CDS             complement(39053..39412)
                     /locus_tag="OO470_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39630..41273)
                     /gene="yojI"
                     /locus_tag="OO470_000519"
     CDS             complement(39630..41273)
                     /gene="yojI"
                     /locus_tag="OO470_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6249878.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41349..41999)
                     /gene="alkB"
                     /locus_tag="OO470_000520"
     CDS             complement(41349..41999)
                     /gene="alkB"
                     /locus_tag="OO470_000520"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6249879.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(41999..43063)
                     /gene="ada"
                     /locus_tag="OO470_000521"
     CDS             complement(41999..43063)
                     /gene="ada"
                     /locus_tag="OO470_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6249880.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43137..44192)
                     /gene="apbE"
                     /locus_tag="OO470_000522"
     CDS             complement(43137..44192)
                     /gene="apbE"
                     /locus_tag="OO470_000522"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6249881.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44304..45425)
                     /gene="ompC"
                     /locus_tag="OO470_000523"
     CDS             complement(44304..45425)
                     /gene="ompC"
                     /locus_tag="OO470_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6249882.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46163..48835
                     /gene="rcsD"
                     /locus_tag="OO470_000524"
     CDS             46163..48835
                     /gene="rcsD"
                     /locus_tag="OO470_000524"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6249883.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            48852..49502
                     /gene="rcsB"
                     /locus_tag="OO470_000525"
     CDS             48852..49502
                     /gene="rcsB"
                     /locus_tag="OO470_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6249884.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(49702..52551)
                     /gene="rcsC"
                     /locus_tag="OO470_000526"
     CDS             complement(49702..52551)
                     /gene="rcsC"
                     /locus_tag="OO470_000526"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6249885.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(52826..53602)
                     /locus_tag="OO470_000527"
     CDS             complement(52826..53602)
                     /locus_tag="OO470_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6249886.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53607..55256)
                     /locus_tag="OO470_000528"
     CDS             complement(53607..55256)
                     /locus_tag="OO470_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6249887.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55257..55816)
                     /locus_tag="OO470_000529"
                     /pseudo
     CDS             complement(55257..55816)
                     /locus_tag="OO470_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(55975..>56359)
                     /locus_tag="OO470_000530"
     CDS             complement(55975..>56359)
                     /locus_tag="OO470_000530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249888.1"
                     /translation="DNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFS
                     EEDIRDINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSN
                     QPLAHTVVLLNSGGDATQTAAILMV"
BASE COUNT        13825 a        15182 c        13832 g        13520 t
ORIGIN      
        1 cgcggtgccg ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta
       61 gaagagcgtt tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt
      121 gacttctcct atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg
      181 tataccttca ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg
      241 ccggacatgc aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc
      301 gttgatctgg aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt
      361 aacaaaccgg cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg
      421 ggcgaaaaaa ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt
      481 cagccgcatg cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc
      541 ttctattttg cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc
      601 ctcacacttc atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca
      661 ctgtcattgt taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt
      721 acgctcccgc atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc
      781 gttcattttg gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg
      841 ctgaatgccc agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa
      901 ctgatgagcc agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac
      961 ggcaatcagg tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc
     1021 ttagcggcaa ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt
     1081 accgaaaaag gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg
     1141 attattcacg atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa
     1201 gcactgaaac gccgccgtga gcgcagcctt acaccgttta ccgtgctctc ctgcgacaat
     1261 attcccgaca atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg
     1321 ccagaactcg ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc
     1381 attgttccgg ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat
     1441 gatccctgcg cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc
     1501 gctgggcgtc ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg
     1561 gaagagatga aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac
     1621 ctctcaggat tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc
     1681 agaacattaa tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca
     1741 caatatgcgg ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg
     1801 caaatcgcga tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata
     1861 catctggggc gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt
     1921 tacgtcagcg gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat
     1981 aaaattcgcg aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc
     2041 ctgcgtgaag ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa
     2101 caagcctggc aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa
     2161 atttaacgat ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc
     2221 ttttctgcct tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc
     2281 gttgtgacca ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt
     2341 cttcatctgt tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt
     2401 ggggaagtcg gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc
     2461 cccggcggct gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta
     2521 ctgcgtcagg gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa
     2581 cagatcctcg atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc
     2641 ttgtgcattc tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc
     2701 cgtgaccagc tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc
     2761 gaaagtgagc aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt
     2821 cacagtgagc atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc
     2881 gagttgcccg ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg
     2941 ttaagcctgc cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag
     3001 gcctgcggct ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg
     3061 gcgcgacttg caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta
     3121 cgaatcaacc gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac
     3181 agccgtattg agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg
     3241 ttgaagcttc gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta
     3301 gagaaaatat gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc
     3361 tgtttctttc ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata
     3421 ttttttattt ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat
     3481 tgactaacaa tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga
     3541 gtttctggct gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca
     3601 tgctattaac tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg
     3661 ccagcccaac attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca
     3721 cgaaagatgc ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt
     3781 cggcattcat gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg
     3841 tggtttttgc atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg
     3901 gttcgatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc
     3961 gattaatcac cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat
     4021 aactcacagt aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc
     4081 gcaccgtgct ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca
     4141 tgcgtttcac tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact
     4201 ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg
     4261 aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca
     4321 cagatttggc cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg
     4381 cattttttta taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca
     4441 aatctcaacc gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc
     4501 tgctttctgc atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag
     4561 tgggtagtga aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta
     4621 atgtcgacgt aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc
     4681 gtctgggcgg cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc
     4741 gtgagcaatt tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat
     4801 ctgtttcccg cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg
     4861 gacgccatgt cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg
     4921 gtgttattat ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc
     4981 gggttctcag ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt
     5041 taactgaaag cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt
     5101 ttccgcgtct tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat
     5161 ttcaggaaat taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc
     5221 ttacctgcat tgtagcaggc gtgatgattg cgatactggg tagctgcctt cagtttttag
     5281 tggcctggca taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc
     5341 tcgataccta ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag
     5401 atacctgcca gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc
     5461 gtacgtttgt gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg
     5521 acattccact caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga
     5581 tcttacccgg cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc
     5641 ctttgctgaa aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct
     5701 tttatagttc acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc
     5761 tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg
     5821 aaactaaaat tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga
     5881 acgatgtgtg gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt
     5941 gctattacct gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc
     6001 gcgaacaatt ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg
     6061 gcctggaagt actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct
     6121 tcattaactt tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt
     6181 taattgcccg cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca
     6241 ttaacattgc gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc
     6301 tcacttccct gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc
     6361 tgaaagagca agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg
     6421 ctattgatga cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg
     6481 attatctgaa aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac
     6541 ccgtacttga cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag
     6601 gggtcgaaac gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag
     6661 gctactggat tagtcacccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt
     6721 atacgccgca gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg
     6781 attgcggcta atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc
     6841 tgtttatcgc tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct
     6901 ttgccgtgct gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc
     6961 ccgccgcgcc aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca
     7021 accgctatgc actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat
     7081 ttacgacttc cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct
     7141 atgctgacga ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg
     7201 gttctcccat tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg
     7261 tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg
     7321 tgcgcattga gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg
     7381 tatttccaga aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc
     7441 ttgccagtgg tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc
     7501 gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca
     7561 ccattcgcta cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg
     7621 cctttgattt gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa
     7681 atttcgataa aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc
     7741 gctggctggc gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa
     7801 tcactctcgc ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc
     7861 gcacgaacag ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg
     7921 agctggtgct tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct
     7981 accagccgcc ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca
     8041 aacttctcaa cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg
     8101 gtcagccgct cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc
     8161 cgttccagca cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact
     8221 cgcaaatcac taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg
     8281 cgatgccgtg gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca
     8341 cttataatgc ccccggcgtg caaagcccgg ttatcgactc actgatcaac caaattattg
     8401 ccgcgcaggg aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa
     8461 cgtggaatta ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg
     8521 ataaattctc ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt
     8581 atgacgttaa taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg
     8641 cttacctgat tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca
     8701 actttttcat cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg
     8761 agtttggtaa tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg
     8821 cgcaaacggg tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc
     8881 cagaagtgat cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt
     8941 acttcaaaat gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg
     9001 cctcggtgct gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga
     9061 gcacgctgat tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg
     9121 ggagtcgctt tgacgtctgg agtagcacat ttatcatcat cggctacgcc attccggcct
     9181 ttttgtttgc catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc
     9241 ctctacgcgg cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg
     9301 attatctgtg gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc
     9361 tgaccatgct gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg
     9421 cgcgtgcgaa aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca
     9481 tgctgctggt gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc
     9541 tgctgattga ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg
     9601 tctcgcgcga ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc
     9661 tgctgaatat tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg
     9721 gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa
     9781 ccgtcgcggc tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc
     9841 tgaacttatc gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc
     9901 gttgttgaaa aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta
     9961 tcaggacccg tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat
    10021 tcgctttggt gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc
    10081 ccggcaaaac tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta
    10141 tggcacgcgg atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg
    10201 cgtgctggcg ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg
    10261 ctttattgaa gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt
    10321 cgtacagcct aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct
    10381 ggtcggcgtg gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc
    10441 acaggcgctt ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat
    10501 ggtcgcgacc ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc
    10561 gctcgatttc ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt
    10621 acaagggaaa aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc
    10681 gatattattg tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa
    10741 taaggcggtg tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc
    10801 gccatcagca aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg
    10861 aaacgctggc gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt
    10921 tacgcctgct cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg
    10981 aatcgctgct tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca
    11041 tgatttttca ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt
    11101 atgaagtgct ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta
    11161 actgccttga tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc
    11221 agctctccgg cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg
    11281 aattattaat tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt
    11341 tacagctgtt gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata
    11401 acctcagcat tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg
    11461 tcgagcaaaa taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc
    11521 tactcaacag tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc
    11581 tggatgttga acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg
    11641 tggatcataa tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg
    11701 gtttagtggg cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga
    11761 ttacctctca gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc
    11821 agctgttacc tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca
    11881 acccacgact caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc
    11941 tttctgccgc acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc
    12001 ctgaaacacg ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga
    12061 ttgccagggc attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac
    12121 tcgacaaaac ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc
    12181 aactggccta tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg
    12241 ttatcgtact gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca
    12301 caccgcagca ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg
    12361 tagtttgaaa agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca
    12421 aactcgtcct ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat
    12481 ggaacttcga tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca
    12541 ccatccgggc taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc
    12601 ggaatcggtt caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt
    12661 cgatggacga ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc
    12721 acaatttgtt tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa
    12781 agattgatgc ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt
    12841 ggtcacgttt gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta
    12901 caatgatgtg actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat
    12961 gatagatttg tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa
    13021 tagatcaccg ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc
    13081 agaatgcaat tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg
    13141 cgccaacaat tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg
    13201 ccatatgggg aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc
    13261 agcccacaaa cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcggcgcac
    13321 tgatgaccat ccacgcagcc ataataaatt gtatccacaa ccccgagcgg aacatattta
    13381 acgcgccaat gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa
    13441 tgtttagcgc aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa
    13501 caaacggtcc ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca
    13561 tgtagctcag gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa
    13621 tgtaaaatgg ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg
    13681 ccgaagccag aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca
    13741 ccagcaccca gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga
    13801 caaacgacat catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga
    13861 taaccacgct ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat
    13921 cgatggtttg tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa
    13981 gaaccaccgg cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg
    14041 caaagcccag aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact
    14101 gcgctgaaat taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg
    14161 ccagcaggcc aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg
    14221 gctcctgaaa gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac
    14281 gatattcacc gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca
    14341 gctcaaccac gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt
    14401 cgctgatggt cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga
    14461 gatctttttc gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta
    14521 caggtgattc cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc
    14581 ggtgcgacca ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac
    14641 gtcctgcggc gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt
    14701 gatccgggtc gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg
    14761 gaccgtgttg ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt
    14821 tgaacgctgc attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac
    14881 gcccggcaat agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt
    14941 gtgtgatttt gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt
    15001 ctgcccagta tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt
    15061 agcagaataa tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa
    15121 gccgtggatg ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg
    15181 cccaccggcg caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc
    15241 gtactggtgc tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg
    15301 ctggggctgg aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa
    15361 gtggtatttg gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt
    15421 tcgctgttga ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag
    15481 caaatcctca ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc
    15541 acgccttttc gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc
    15601 ggcgatgaga aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt
    15661 aaacacggct atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc
    15721 agccgcttgc aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg
    15781 aaaaaacagc aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag
    15841 cgcaaagggg taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta
    15901 ctgcctgccg aagatgctgc actgattact ggcgacaacc ccggcgctga gcgcgatgtg
    15961 ttaattgaag attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc
    16021 accggatttg acgccccgca cgtcgatctt atcgccattc tgcgccctac tgaatcagtg
    16081 agtctttacc aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt
    16141 ttaattcttg attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg
    16201 aaaggcaaaa gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac
    16261 accttttggg ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt
    16321 cagggatggt ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt
    16381 aaaaattgcc cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt
    16441 gacacggtac tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg
    16501 ctggtattac gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg
    16561 ttgaaaatca cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa
    16621 acgcccgccc agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg
    16681 ggcatccctc tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga
    16741 catccggatt ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg
    16801 ttcgattacg aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga
    16861 ttgatgtata aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc
    16921 acttacctgt tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac
    16981 tatcaacgca gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc
    17041 taacaagttc ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga
    17101 tcacgacaaa gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctaaccat
    17161 cgttgttgac ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa
    17221 accgaagctg cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac
    17281 cccgctccgg cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt
    17341 ttgcaggcca gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat
    17401 tgccgccaga cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg
    17461 tcaaggtatc ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt
    17521 taatagtcaa gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt
    17581 ctgccgggaa gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca
    17641 cttcgcttac gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct
    17701 gcagctgctc attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat
    17761 ccagttgtgc ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt
    17821 tcgcgttcaa tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc
    17881 ctactcgccc tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg
    17941 ttaaatcgat acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga
    18001 tgtcgagatt ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcaaat
    18061 actccaccgc cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat
    18121 atttcgctaa ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct
    18181 cttcgccctg acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc
    18241 cgtaagcttt atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg
    18301 tttctgtcgg ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct
    18361 tgataagctg gtgcagagca atctggttga tatccagact catgataaac tctcctttaa
    18421 gaccgggcgg tattcaacca ccgccagtta agcgacgcaa taaaagatgg cggtcatttt
    18481 acagaaggca gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc
    18541 tacggtaata tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc
    18601 gctacagtaa tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac
    18661 ataaggctcc gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca
    18721 acaccagcat tgccccggcc caacgccagg caattgccaa ctcttttgcc agcgccttac
    18781 agtcctctat caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact
    18841 catcgtcagc gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca
    18901 ctgttcaata ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg
    18961 ccgacaacgc ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc
    19021 ctagtgttcg ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag
    19081 aggctgatga ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc
    19141 gatagcgaag tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt
    19201 atcaacccag acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg
    19261 gttattttat tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg
    19321 acgcgtcgtc gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc
    19381 tcgcatgtgg tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc
    19441 gctaacctgc cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg
    19501 cttgatgcgc aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc
    19561 gtacagtatc cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg
    19621 ttgattactg tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca
    19681 ggttttgctg agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac
    19741 aacggcatct ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg
    19801 acccgtacgc ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta
    19861 ttctcatcag atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg
    19921 atgccgagcg tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg
    19981 ggccgctacg cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac
    20041 attgacgaca gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc
    20101 gatgaccaga tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg
    20161 gtggtgatta tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac
    20221 tggtcccacg gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag
    20281 cgtattaatg ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg
    20341 catgtcagca cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc
    20401 cgtcattact gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag
    20461 acgctggtgc tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg
    20521 aaagatgaaa aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt
    20581 atcgctaact gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga
    20641 aaacggtagt attagcagcc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat
    20701 ggggtgtcgg gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa
    20761 ccaaccctta cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac
    20821 atcacagcct gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc
    20881 acggcgattc tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg
    20941 gggtaaacga tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat
    21001 taaatttgcc atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg
    21061 cggctttcag ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg
    21121 ccgttcccgc ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg
    21181 ataacgtgta gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac
    21241 tccacaatcc gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat
    21301 tgtaatcgcc aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac
    21361 ggtcaacttt caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag
    21421 cccccagggt atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat
    21481 gagagtaacc aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat
    21541 cgataccgag cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca
    21601 cgttgttgcg ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta
    21661 cgctaccaag acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg
    21721 ctgccatgtt cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc
    21781 tccagctatg gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc
    21841 ctccggcatt gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc
    21901 tatctcctgc tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg
    21961 cctgaccggt gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa
    22021 aattgccgtt tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg
    22081 tcacagatga acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa
    22141 ggttggctaa gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc
    22201 atgcgctatt ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa
    22261 aagaagcgcc ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt
    22321 gcggcttcat gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc
    22381 cagcccagac attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg
    22441 aatacgtgat acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca
    22501 ctttcgtacc agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt
    22561 caatggcctg cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt
    22621 ttgacacacc atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg
    22681 cattcgttga gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc
    22741 catccataac ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt
    22801 cttcaactcg caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga
    22861 tatcgaactg accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa
    22921 tagcaccgcc tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta
    22981 gtcctccctt ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc
    23041 cgcctttaat cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgagag
    23101 tagtctggtt tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt
    23161 attctgcggt accattaatt tcaatgtatt gataccctcc gttaatttca gtattactac
    23221 ttacaccaaa ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg
    23281 tcccataaac cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact
    23341 caactgttga tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg
    23401 cggaacgaca aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta
    23461 attgcagtta aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt
    23521 ttaaaaactg tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg
    23581 atgagaagct gatgcaaaat tccgtcttta taataaaaat gatgccaaag cgaacgacaa
    23641 ggttgtagtt ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag
    23701 cgttaccaac gctacgctca aacataatga ttctaataaa acctcaggag actactatgc
    23761 ctgaagcaac gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg
    23821 ttcgtcagtt actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgaaggcg
    23881 cgagcgcgat cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata
    23941 tgaaaggtat gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc
    24001 aaattattat cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag
    24061 gcgcagacgg ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta
    24121 ccggagcgaa aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg
    24181 aaatgtttgg cgcggaagaa gatcccttca gcgtgctgac ggagcgcgaa ctggatgttc
    24241 tgcacgagct ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg
    24301 agcagacagt aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg
    24361 tggcggccac cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat
    24421 ttatcatcgg gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag
    24481 gcctacgtgg ttcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt
    24541 cacgccgcat ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac
    24601 catcgggcct ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata
    24661 ctacgttcaa tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc
    24721 cacgcggcaa cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt
    24781 agcacacgga tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg
    24841 cggttgtcgt tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg
    24901 ctgttcttcg gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta
    24961 cccagcgcca tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt
    25021 tgcagttgag tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg
    25081 gctttcgggt ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag
    25141 attttcacct gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc
    25201 accacaatac ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt
    25261 tcaggaaacg cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa
    25321 ccgccaatgc caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac
    25381 ggcggatcgt aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt
    25441 ttacttttac cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc
    25501 gagttggaat cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc
    25561 tgacggaact gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc
    25621 gctgagccgg agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct
    25681 tactgtattt ctcccacagc ggcttgatct tttcttccca gacgcgagga ttgagatcgc
    25741 ccgcatggcg atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat
    25801 agacaccgag atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat
    25861 tgcccagctc tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga
    25921 ccacgcgaat gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg
    25981 gacaccaagt cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct
    26041 gataaaactg ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa
    26101 tgagcgccga ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca
    26161 gcagcgccgc ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca
    26221 tacggcctcc ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag
    26281 acacagcagt ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg
    26341 tttgtagtaa agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta
    26401 caggtcacgc gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt
    26461 gtaataacgt ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat
    26521 agtcgccaca ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa
    26581 gcgatattca tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct
    26641 ataattctgg ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac
    26701 catcccccaa taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc
    26761 agttgccagc accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac
    26821 tttgctttcg aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat
    26881 aatcaataaa ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac
    26941 accaagcagt agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc
    27001 gccaatcgaa atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag
    27061 caacaccacc agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg
    27121 cgaccacagc gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag
    27181 cagcgaaccg ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc
    27241 agacgcaaac gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag
    27301 acacaacgag aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg
    27361 ttcagtgacc gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc
    27421 gttttctacc ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc
    27481 ggaaccgagc acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt
    27541 aaaacgcgca taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa
    27601 cgccaccgag aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg
    27661 atcctgtaac agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga
    27721 gaacgggtta gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat
    27781 cgccagtaca cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa
    27841 ggtccagccg ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaagt
    27901 agccgccacg cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac
    27961 ggtaaagtcg ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat
    28021 aaacagcagc caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac
    28081 gccccatagc ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc
    28141 gtttccaatt tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg
    28201 ttagcttcca tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc
    28261 acttctttcg cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc
    28321 ccttcacgga acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca
    28381 tcgtaaatgg tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc
    28441 accatcccgc caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg
    28501 ccgtagagaa tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc
    28561 accagaccga tagtcagcgc tagccctgcc aacacggcac aggcaatcca caagcggttt
    28621 ttacggcgaa tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg
    28681 cccgctgttg cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca
    28741 aaaccaccag cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc
    28801 ccattgcgaa aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag
    28861 tatcagttca ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg
    28921 catcagcgtc agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga
    28981 acgcatcgcc ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca
    29041 ccactccacg gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc
    29101 tgccgcacgg cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac
    29161 atacaaaaac agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc
    29221 ccacatcggt tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc
    29281 aatgggggcc atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat
    29341 aaacgccgcc accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg
    29401 caggtagata atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc
    29461 ccagatccag ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca
    29521 gccacagatt tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt
    29581 ttcgatacca gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac
    29641 gctgccgccg tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt
    29701 gccagatacc catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag
    29761 atgagtaatg ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta
    29821 agtcccactg tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc
    29881 agcagcgtca gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc
    29941 agtggcgaaa ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc
    30001 agcacaacgg cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc
    30061 aaatcgtcac ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag
    30121 ataatgcccg gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa
    30181 agggtaatta caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac
    30241 gctacgcgca gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca
    30301 gtgaaatgcg gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca
    30361 caatcccccc ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa
    30421 cgtcaatcgt ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac
    30481 gcgctaaagc gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga
    30541 atccggcaag cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat
    30601 gataaaagtg cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta
    30661 tccataacag ggtttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga
    30721 gaacctcgcc agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc
    30781 ccgcgccgtt gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc
    30841 cactaaataa ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca
    30901 cgcaataacc ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga
    30961 tccctttatg gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg
    31021 cggcaaccga tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact
    31081 cctgcgagtt attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag
    31141 cttcaaattt ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg
    31201 ctttgagctt gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg
    31261 tcgcacggac gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg
    31321 tgttgcgcat ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg
    31381 tgttaaagcc cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca
    31441 gcgccagatg gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac
    31501 ggtcagaatt tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat
    31561 tccccacgat tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac
    31621 gcggagccac ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac
    31681 gcggcgcgcc agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat
    31741 tggtcgttac ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt
    31801 tcagcggcat ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata
    31861 cttccggcga ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca
    31921 gggccagcat cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt
    31981 cgctcccgaa ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca
    32041 gcgaccgcac gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac
    32101 cggggcacgt agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca
    32161 tttctgccga tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag
    32221 cgcgccaacc ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa
    32281 aaacagcgcg agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat
    32341 cagagaaacg gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag
    32401 aatgaccacc aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc
    32461 aaagcgcctg cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct
    32521 gcaaaataat cgctttcccg ccagggcgta gagcacggtg ataatcaccg caccggtcaa
    32581 cgccaccgtg gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt
    32641 cagcggaacg gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc
    32701 aaaccatgga ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac
    32761 cagccaacgg tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg
    32821 tttacgattt gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc
    32881 ctttcgccag tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt
    32941 cgcacttacc gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct
    33001 taccggtacg cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt
    33061 cgcgataaca aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca
    33121 gtaccgccag ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc
    33181 ttgggcacac tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga
    33241 caaaatatgg cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt
    33301 aagggcaagc ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt
    33361 ttatggctcc gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc
    33421 ccagcgccac acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc
    33481 ggcggcgacc attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt
    33541 tgaccgcgca cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca
    33601 gtttgttaac cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac
    33661 ggttacgacc gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca
    33721 ctttgtcgcc acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg
    33781 cttccgggaa ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt
    33841 gctccagaac gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg
    33901 ccggttcgaa cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat
    33961 agccttcgcc cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt
    34021 taccattcac caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca
    34081 gatcgtgacc gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat
    34141 agaagcccag ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga
    34201 atttgctcac ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg
    34261 gtttcttcgc cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact
    34321 ggactaactg ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga
    34381 actgagtacg gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg
    34441 gcaggatcaa gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga
    34501 agttgcgcgg atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca
    34561 tgttgttggt acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt
    34621 gtgccaccgc atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct
    34681 tctcacagat atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag
    34741 caaaggtgcc cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg
    34801 gaccgcaacc cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt
    34861 tagcccacac accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct
    34921 tgttcggatc ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca
    34981 tttcagcggt tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca
    35041 tcggttcgga ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac
    35101 gtaaaccgta gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt
    35161 cctgatttat cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt
    35221 cagattgcgg cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg
    35281 tagaaagcac cgccacggtg acgttctggt tagagagacg actgttagtg atgcgtgacc
    35341 agaggatcgg gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct
    35401 cgatgtcgtc atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta
    35461 ctgccgacgc catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt
    35521 tgaacagctt ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac
    35581 cgatcgattc cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga
    35641 aggcctgatc ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca
    35701 tacgcagcag cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc
    35761 ccttaatgca gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca
    35821 cacgcccctg ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg
    35881 ctttatccca tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc
    35941 tgagaccggc agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac
    36001 tgagtttcat ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc
    36061 cagcacgccc tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc
    36121 tgcttccacc accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc
    36181 gggaaaggcg ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac
    36241 caggctgcaa acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg
    36301 gacagctggc tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt
    36361 agatcccgga gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc
    36421 ggcggcattc aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc
    36481 aggccctggt gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac
    36541 agaagctgca ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca
    36601 gaatgttgtt ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat
    36661 gagattcatc accggaccag ggcggacgga taccgttact ggctttgcgc cagcaaccag
    36721 tgagtatgcc ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc
    36781 tgaaaattga gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa
    36841 gatattaaag gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc
    36901 aaaagagtgg cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa
    36961 acatatttat atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag
    37021 aatttaacac taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc
    37081 aaattaataa atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa
    37141 atgaagaagg aaagcaaaaa aatgaagacc attctacctg aagtattgtt tgccgctttc
    37201 gctaccactt ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat
    37261 ccacaagctg aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa
    37321 tctaccctga aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat
    37381 cgtctcggcg ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc
    37441 tttgataaag ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag
    37501 aagaaatttg tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg
    37561 ccgatcgtgg tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa
    37621 gagaaaattg acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt
    37681 ggataaggcg gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg
    37741 ctggcgcgtc ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat
    37801 tacaacgcaa tatccgccac ttctttttga acgggttgcg gtttcggcgt actatctgct
    37861 gcttgcggtt tgtcgtattt cagccccagc acttcgctgg tgtactgcaa ctcgcgttct
    37921 gttgccgcta catcaccgtt cagcttgcgt ccataagacg gaacgatcgc tttcaacgta
    37981 gcctgccatt gcgggctgga aacgcgatcg ccaaatactt tttccagtag attcaacata
    38041 atcggcgcgg cggttgacgc ccctggcgat gcccccagca gcgccgcaat ggtcccttgc
    38101 tggtcactga ccacttcggt acccagacgc agtacgccac ctttctcggc atcacgcttg
    38161 ataatctgca cacgctgccc cgcttgccac aaacgccagt cctctttttt cgcttgcgga
    38221 tagtactctt tcaacgcttc aaaacgatcc tcttcactca acatcacctg actcaccaga
    38281 tatttcacca gatcgaaatt atccagcccg acgtgcatca tcggcatcac gttagaggtg
    38341 gtggtggaac tcatcagatc ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg
    38401 gcaaatggcc caaacagcac tacgcgttta ccgtccagaa cacgggtatc gatatgcgga
    38461 accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa ctttcgccag atggcgatta
    38521 accacgtctg gattttccga aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct
    38581 ttcgcttccg gaatccccga ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata
    38641 aagacaaatt tggcgcggat gttctgtgca gtgccgtttt tcagatcggc aacggtaacg
    38701 gtccaggtgt tatcgtcatt acgctttagg gcgcggactt cgctgctgag ttgcagcgag
    38761 aagttagatt tcttctgtaa ggaagcaatt aactggcggg tgatctcacc atagttcaca
    38821 tcagtgccaa tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
    38881 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
    38941 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
    39001 cggtattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
    39061 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
    39121 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
    39181 tgttctagcc gttcgaactc tcctgcgcgc ccccctccag gcgctccacc atggtcatcg
    39241 accattcagg ctccagctcg cgtcaatagg tccccaacgt ggcgctcata atgccgccgc
    39301 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
    39361 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
    39421 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
    39481 aagtaacaac atttaaataa tgtttataaa ttatattcta attttagaaa cattataatt
    39541 tcgttgatta attatagggt tattagaaat gaagggattt ttagcatatt gacctgacgg
    39601 cagcagtccg ccaggtcagc gtcgtaatat tatgccgtcc gggcaacggc atcacgcgaa
    39661 gcggcatcgc gctcttcgcc cgtcagctcg ctaagttgcc cattgcgcat ttccagcagg
    39721 cggtcggcgt ggataaagta atgatcatca tgactgatag cgaaaatagt tttacccatc
    39781 tcctgcatca gcggcagcaa cacctgataa aactcacgac ggaagtgtgg atcctgatcc
    39841 accgcccatt catccagcag gataatatcg cgttcttctg ccagcgccag caacagcgcc
    39901 acgcgttttt tctgcccttt tgataacttc aggttaacaa tacgcccgtt gcttaactca
    39961 agcttatgag ccattttcag ctgcgccagc cacttctcaa ccagttgcgg gttagcgggt
    40021 tgcccctccg gccccagcag ttgatcaaac agccagacat cggtaaacac tgccgaaaac
    40081 agtttgcgat aatcttccgg ttgttcgccg ctgacaggtt tgccatccag caagatttcg
    40141 ccgctttgtg gctgatacaa gcccgtcaac aacatcgcca gcgtcgattt tccgctaccg
    40201 ttaccgccaa tcagaaacag cagctcgcca cgtttgatgg tgagattaat cggaccaacg
    40261 gaaaacgcgt tatcctgata agcaaacgtc acgttacgca gctccagcgt ttgccagttg
    40321 ggaaacgcct gcgggcgcgg aaactcagct ttgaaaggcg cgagcgcgaa tttgttcagc
    40381 ttgttaaacg ccacctgcgc cgtcagcagc gtcggcaatg cgccaaccgc cgaaagcagc
    40441 ggcgtacgca ggaataacag cgtcaacgaa taggtcgcag caacgttggt atcagcccaa
    40501 ccgagactgt tcgccatcca gaacaccagg ccgatagcgc ccagcatcat gatgtttgac
    40561 cagttcacgg cactaagatg gaaggtgtct gcgcgaataa tatgatggcg atactcttgc
    40621 gcatcgggga tgtagaggtt gttaaacaca tactcggcgc gttcccggtt cagagtcagc
    40681 tctttgcgcc cttcaagtac agtttgaaaa tccgtgtaca gcttgtcttc ggtttcacgt
    40741 agggtcgcca tatgtttgta cacccgcgcc accagcacaa aaccgcccca gatggtgatc
    40801 gccatccaga tagccgttac cagcaacatt ttgcccgaca gcatccacag atacgccgat
    40861 gaaccgatag tgagaatgat cccctgcccc agttccggca gacgcacaaa agcaatggtg
    40921 atattgcgca catcgctggt taatcccgcc agcaacgagg cgctaccgag ttgttcaatg
    40981 cgctcgacgt gggtatccag aatccgcttg ataaattcac tacgcagtcg gtaaacgaag
    41041 tgatgcccca aagtggtgag cgccagttgc gatccgagag tgactgccat caacagcagc
    41101 aataatccca gaaactccgg taacaccagc agactggtat ccgccgtttc gataaggcgc
    41161 tgattgataa aagcaattaa gccaatgcct aatgccgcac tggcgaggct tagcgccatc
    41221 acactgataa atggccagcg atactgccgc cagacaagta caagaagttc catgcagaaa
    41281 acccggacaa tgaattacag cccgcagttt aaacatcttc gcgcgcacag caataataat
    41341 tcttattttt attctttttt acctgcctga cggaatgtca ggttgtagcg gcagtcggtg
    41401 gtgagtggat gaaaccccgc tttcaacggt tgaataccgt gataaaacag ccgcgattca
    41461 ccgccccata ccaccacatc gccatgttcc aacaacaaac gtttgagcgg atcattacgt
    41521 ttcaggccac caaattgaaa aatcgcgggt aagcccagag aaacagaaac aattggcgcg
    41581 cgcagatccg gttcgtcttt atcctgatgc agcgacagtt tcgcgccagg agcgtagcgg
    41641 ttgataagac aagcatctgg ctggaaatct ggatagcccg ccgccgtagc cgcacgttga
    41701 cataaatcat gaaagctttg tggcatggcg ggccacggtt tatttgtttg cggatcaatg
    41761 ggcgaataga gataaccttg ccgatgggtc gtccagccca gatgcccaca gttggtcatc
    41821 gccaccgaca tggtatatcc gcctggagcg accatctggc gaaacggcga ctggctggca
    41881 acgtcattaa tatcgcggat tagttgctcc gcagcattaa aagcaaaacg ccgtaaaatt
    41941 accgcaccag ccgccagtgg ctcttgccac ggttcagcat cggcaaacag atccaacatt
    42001 acctctcctc attttcagct tcgcggcgca gcagttgcgc tttacgcgac acgccccagc
    42061 ggtaaccgga aagtgtgcca tcaccacgga ccactcgatg acagggtatt acgatagcca
    42121 gcttgttggc ggcacaggcg ctggcaacgg cccgtaccgc tttcggtttg ccgatggcgt
    42181 tagccagttg ctgataactg acggtttcac cacaaggtat cgtgcgcagt gcctgccaga
    42241 cttgttgctg aaaagcagtg ccgcgaatat ccagcggtaa cgtcagcggc gtatcgcgtt
    42301 gattgaggct ggcgatcact tcacgcacat gttgctgaaa cgtcagatcg gcaggcgcgt
    42361 tgtcggcagc gggaaacatc tgctgcaact cgctgattaa tgtcgcgtca tcatcgccca
    42421 gcaatatcgc gcaaatcccc cgctcgcttt ctgccaccag gcaacgaccc agctcacaat
    42481 cagccagcgc gtaacgcacc gccagatttt cgccaccgtg acggaattgt ttagccgtca
    42541 tgcccagcgt ttcatcggct ttgcggtagt aactgctgct gtcggggaat ccggcgttaa
    42601 gaatagacgt cgtcacgctc tcccctttcg ccagcgattc gcgcaaacgg cgagcgcgcc
    42661 aggcctgttg ccaggcttta ggcgtcattc cggtagtagc tttaaacaac cgatgtagat
    42721 gaaatggact catcgccacc tggtcggcta aggcttccag cgttacaggc gtttcctgtt
    42781 ccagcagtcg acacgcgtgg gtgattttat ccaaccgatg ttgccgggga ttggctttgt
    42841 ctggctgaca acgtttgcag gggcgaaatc cggcggcgag tgcctcgctg gcatttgcgt
    42901 agaaggagac attttcccgc aaggcatgtc tggcgcggca agaaggacgg caaaagatac
    42961 ccgtggtgcg cacggcgaaa acgaattcgc cgtcggcatt cgggtcgcgg gctaagacag
    43021 attgccagcg ttgatcgtca gttaagcatg tggcgttttt cataatcagc tccctggtta
    43081 aggatagcct ttagtctgcc cggtcaccat cacgcaaaaa ccaacaatct tgcgctttaa
    43141 tttttttcgc tgacaaggaa gcttttaaac tgtggtgaca tccaggtttt aaagctatcg
    43201 ccttctttgg tgatcatata aaccgccagc ccttcccggc gaacaacctc tttggctttc
    43261 tccggcccga gtaccatcaa gccagtatcc caggcatcgg cttccagcgc cgtcggagca
    43321 atcaccgtca cggataccag attgtgttcg atgggacgcc cggtttgcgg atcgataaca
    43381 tgggaaagac gtttgccgtc cagttcgtaa tagttacggt agctgccaga ggtgctaatc
    43441 ccatgaccgt tgatatccac cacagcctga accgcgtttt ctttatcggt tggtttttga
    43501 atcgctaccc gccacggctg gccttcaccg ttcataccac ggctgttcag cgcgccgccc
    43561 accgacacca gatagcggga aatcccttcc tgctccatca agcgtgccag gtgatccgcc
    43621 gcataacctt caccgacggt ggagagatcg acatataaat ccggcaggtc tttttgcaga
    43681 tattgctgat gcgactgatt gatgaccgtc aggtgctgta agccggtttt ggctttcatc
    43741 gcatcgatct gttcctggct cggaatttga accggctgtt gttccgggcc aaagccccac
    43801 agattcacca gcggcccgac ggtaatatcc atcgcaccat cggtccttgc cccaatgcgc
    43861 agcgaggtgg tgacgatatc ggccatcgct tcacttaccg gccacggcga caaactttgc
    43921 gagtcgttaa agcgcatcag cgcggaatct tttttatagg tcgaaagcag ctgatcgtcg
    43981 gcgtccagct gggtctgaat cttttcttta agttcggcac tgcgtttggc atcaatgccc
    44041 gggatgctgg cacgccagaa ggtacccatg gttttgcctt caagaacggt aacttcagta
    44101 gcgtgggttt tggcgggttg tggtttttga tcgcaaccaa caaagaagag cgcggcaacc
    44161 agcagtgcca cgcgggtaaa gcttatttcc atacgtgatt atcctcatgc gaacggtcgc
    44221 aagagtacac caaaaaactg tgtttgtacg ctgaaaacaa tgaaaaaagg gcccgcaggc
    44281 cctttgttcg atatccatcg agattagaac tggtaaacca gacccagagc tacgatgtta
    44341 tcagtgttga tgccagcgtc acgagtgaac tggttgtcgt ccagcaggtt gattttgtag
    44401 tcaacgtagg tggacatgtt tttgttgaag tagtaggtcg caccaacatc aacatatttc
    44461 aggatatctt cgtcgtcgta gttacgacca ttgatgacac ccaggttttt acctttagac
    44521 tgcaggtaag ccagggacgg acgcagaccg aagtcgaact ggtactgagc aacagcttcg
    44581 aagttctgtg ctttgttcgc ccaacccaag ttgcctacgc gagttgcgtt gtaggtctgg
    44641 gtgtactgag cagctaggta gatgttgttc gcgtcgtatt tcagaccacc ggtgtaggtt
    44701 tcagcgcggt cgccattacc gatgtaacgc tcatcataac ggttcagacc gaagttctgg
    44761 tcatcagtac gtttggagct ggaaactgca gcaccgatac cgaagccttc gtaatcataa
    44821 gtaatagaac cgccaacgcc gtcaccgttc tgacgcagag cttcacgacc gttgttggtc
    44881 atgccttcac cttccgggct accgtttttg ccctgatact gaacagcgaa gttcagaccg
    44941 tcaaccagac caaagaagtc ggtgctacgg taggttgcga agccgttacc acgctgctgc
    45001 atgaagttgt cagaaccgta agtgtcacca ccgaattctg gcagtacgtc ggtccaggaa
    45061 gttacgtcgt aaactacgcc gtagttacga ccgtagtcga aagaacctac atcctggaat
    45121 ttcagacctg cgaatgccac acgggtccag gagttgtttt cgttttcagc gctgttgccc
    45181 tggatctgat attcccactg gccgtaaccg gtcagctggt cagtaacctg agtttcacct
    45241 ttgaagccaa gacgcatgta ggtctggtcg ccatctacag acttgtcgtc agagaaatag
    45301 tgcaggccgt ctactttacc gtacagatct aatttgttgc cgtctttgtt gtaaacttca
    45361 gcagcgtttg ctgcgcctgc taccagcaga gctgggacca ggagggacag tactttaact
    45421 ttcatgttat taaccctctg ttatatgcct ttatttgctt ttttatgcca ctgcatactg
    45481 attaaccctc attaatcagt cggcaagtcc attctcccca aaaatgcaga ataatccaac
    45541 acgaatatga tactaaaact tttaagatgt ttcatttatc gctatagatg tttcaaaatg
    45601 taaatacaag ggaacttttt aagattattg cggaatggcg aaataagcac ctaacatcaa
    45661 gcaataataa ttcaaggtta aaatcaataa cttattctta agtatttgac agcactgaat
    45721 gtcaaaacaa aaccttcact cgcaactaga ataactcccg ctatcatcat taactttatt
    45781 tattaccgtc attcatttct gaatgtctgt ttacccctat ttcaaccgga tgcctcgcat
    45841 tcggtttttt tacccttctt tacacacttt tcattattct gtgctaccac agaaaaacta
    45901 taacgcttgt taactatttc acaaataatt aacatcagca taatttccag caatctttgt
    45961 ttatttgcaa ttaatttcgt tgggcttttt gtaggttatt tgtacagcaa aatggcgctt
    46021 gtacatctat ttccccccat acaggatgat aaatatcacg ggagaataga gaatcatcaa
    46081 tcaggtaaga gtctggaatt tcacactgta ccctttatac tgccctatca cttcgcgaag
    46141 ttttaacagg tcataaacac gaatgcgtca gaaagagaca acggccacga cccgcttttc
    46201 actcctaccg gggagcatta cccgcttctt tttactgttg atcattgtgt tactggtgac
    46261 gatgggggta atggtacaaa gtgccgttaa cgcttggctg aaagataaaa gttaccagat
    46321 tgtcgacatt acccacgcaa tccaaaagcg cgtcgatacc tggcgttacg tgaccaggca
    46381 gatctacgac aacattgccg cgacgacctc cccctcctcc ggcgaaggtt tacaagagac
    46441 gcgcctgaaa caggatgtct actatctgga gaaaccacgc cgcaaaacgg aagcgttaat
    46501 ctttggctct cacgacaact caacgcttga gatgactcag cggatgtcca cttatctgga
    46561 tacattgtgg ggtgcagaaa atgtaccgtg gtcgatgtat tacctgaatg gtcaggataa
    46621 cagtctggtg ctgatctcaa ccctacccct taaagatctc acctccggat ttaaagaatc
    46681 gaccgtcagt gacattgttg attcacgtcg tgcagaaatg ttacaacagg ccaacgccct
    46741 cgatgaacgc gaaagttttt ctaacatgcg ccgcctagcc tggcagaacg gtcattactt
    46801 taccttgcgt accacgttca accagccggg acatctggca acggtcgtgg cttttgatct
    46861 gccgattaat gatttgatcc caccgggtat gccgctggac agtttccgcc ttgagccaga
    46921 cgcgacggca acgggaaaca atgataatga gaaagaaggg acggatagcg tcagtatcca
    46981 ctttaacagt acgaagattg aaatctcctc ggcactcaac tctaccgata tgcgcctggt
    47041 ctggcaggtt ccttatggca ccttattgct ggatacgttg caaaacattc tgctgccact
    47101 gctgctgaac atcggtttgc tggcgctggc gttatttggc tataccacat tccgccattt
    47161 ctccagccgc agtacagaaa gcgtccccag cacggcggtc aataacgaat tgcgcatttt
    47221 acgggcaatc aatgaagaga tagtctcact gttgccgctc ggcctgctgg ttcacgatca
    47281 ggaatcgaac cgcactgtca taagtaacaa aattgccgat catttgctgc cgcatctgaa
    47341 tctgcaaaac atcaccacca tggcggaaca gcatcagggg attattcagg cgacgatcaa
    47401 taacgagctg tatgagatcc gcatgttccg cagccaggtc gcgccgcgca cacaaatttt
    47461 cattattcgc gatcaggatc gcgaagtgct ggtaaacaag aaactcaagc aggcgcagcg
    47521 tctgtatgag aaaaaccagc aggggcggat gacctttatg aaaaacattg gcgatgcgct
    47581 gaaagaaccc gcacagtccc tggcggagag tgcggctaaa ctcaacgccc cggaaagcaa
    47641 acaactggcg aatcaggcag atgtgctggt gcggctggtc gaagaaatac agttagcgaa
    47701 catgcttgcg gatgatagct ggaaaagtga gacggtgctg ttctccgtgc aggatttaat
    47761 tgatgaagtt gtgccttcag tgttgcctgc catcaagcgt aaaggtctgc aactgctgat
    47821 taacaatcat ctgaaagcac acgatatgcg ccgcggcgat cgcgatgcct tacgacgtat
    47881 tttgctgcta ctgatgcaat atgccgtgac ctcaacgcaa ttgggaaaaa tcacccttga
    47941 ggttgatcag gatgagtcct ctgaagaccg cctgacattc cgcattctgg acacgggaga
    48001 aggcgtaagt attcatgaaa tggataattt gcacttcccg tttatcaacc agacccaaaa
    48061 cgatcgttat ggaaaggcgg acccgctggc attctggctg agcgatcaac tggcacgtaa
    48121 actgggcggt catttaaaca tcaaaacgcg ggatgggctt ggtacacgct actctgtgca
    48181 tatcaaaatg ctcgccgctg acccggaagt tgaagaggaa gaagagcgtt tactggatga
    48241 tgtctgcgta atggtggatg ttacttcggc agaaattcgg aatattgtca ctcgcctgtt
    48301 agaaaattgg ggtgcaacct gtatcacacc cgatgaaaga ttaattagtc aagattatga
    48361 tatcttttta acggataatc cgtctaatct tactgcctct ggcttgcttt taagcgatga
    48421 tgagtctggc gtacgagaaa ttgggcctgg tcaattgtgc gtcaacttca atatgagcaa
    48481 cgctatgcag gaagcggtct tacaattaat tgaagtgcaa ctggcacagg aagaggtgac
    48541 agaatcgcct ctgggcggag atgaaaatgc gcaactccat gccagcggct attatgcgct
    48601 ctttgtagac acagtaccgg atgatgttaa gaggctgtat actgaagcag caaccagtga
    48661 ctttgctgcg ttagcccaaa cggctcatcg tcttaaaggc gtatttgcca tgctaaatct
    48721 ggtacccggc aagcagttat gtgaaacgct ggaacatctg attcgtgaga aggatgttcc
    48781 aggaatagaa aaatacatca gcgacattga cagttatgtc aagagcttgc tgtagcaagg
    48841 tagcctatta catgaacaat atgaacgtaa ttattgccga tgaccatccg atagtcttgt
    48901 tcggtattcg caaatcactt gagcaaattg agtgggtgaa tgttgtcggc gaatttgaag
    48961 actctacagc actgatcaac aacctgccga aactggatgc gcatgtgttg attaccgatc
    49021 tctccatgcc tggcgataag tacggcgatg gcattacctt aatcaagtac atcaagcgcc
    49081 atttcccaag cctgtcgatc attgttctga ctatgaacaa caacccggcg attcttagtg
    49141 cggtattgga tctggatatc gaagggatcg tgctgaaaca aggtgcaccg accgatctgc
    49201 cgaaagctct cgccgcgctg cagaaaggga agaaatttac cccggaaagc gtttctcgcc
    49261 tgttggaaaa aatcagtgct ggtggttacg gtgacaagcg tctctcgcca aaagagagtg
    49321 aagttctgcg cctgtttgcg gaaggcttcc tagtgaccga gatcgctaaa aagctgaacc
    49381 gcagtattaa aaccatcagt agccagaaga aatctgcgat gatgaagctg ggcgtcgaga
    49441 acgatatcgc cctgctgaat tatctctctt ctgtgacctt aagtccggca gataaagact
    49501 aatcatctgt aggccagata agacgcgtta gtgtcttatc tggcatttgc accgattgcc
    49561 ggatgcggcg taaacgcctt atccggccta cgattcccat tatttcaaca gattacatta
    49621 aagtaggcca gataagacgc gtcagtgtct tatctggcat ttgcactgaa tgccggatgc
    49681 ggcgtaaacg ccttatccgt cctacgaatc ccgcgatttc ctgaccctct cggcatatac
    49741 cgtcagcgtc tgttttatca catccagcgt taccggcttc gagaggcagc tgtccatacc
    49801 ggactccaga caccgctgct tctcttcagc caacgcatta gcagttactc cgattaccgg
    49861 caacgtcagt cccaactgac gaatgcgttg cgtcaagcgg taaccatcca tatttggcat
    49921 gttgacgtcg ctaagcacga tatcaatatg attcttacta agtacattaa gcgcatcgac
    49981 gccatcattc gcggttttac attgatagcc caacgatccc aactgatctg ccagcagacg
    50041 tcggttaatc ggatgatcat ccacgaccag aatcatcata tcgtcgttat cgctgaccgc
    50101 tttgtccgtc gacggcagag cgttagcagg atcgtcgctc tccatctcga tcaaataaat
    50161 acgcgccaac aatgccggta gctcatgcgg agcagccaca ctgtgtaccc actcccctgg
    50221 cgctttctcc agcggaatac caatatggcg acgacagaag gtcactaccg ctctgccctg
    50281 ccatttttta ctcactacct cgtcagtgat taacacatct tcgggagtcg gttcctgccc
    50341 ttcgtatgtt gtaacgacga tgccgctgcg ctgcaaactg gtttccagga actgacagag
    50401 cgacgcattg cggaccgcca gccagcagcg tttaccactc aacccttcca cgcctttttt
    50461 ctgcgggtac tgagcgccgt acaacggaat acgcacggta aactggctgc ccattcccgg
    50521 ttctgaatct accgagatat cgccgtccat catgctgatc agtttttcac aaatcgccag
    50581 acccagaccg gtcccctgga agtttcgctg tacgcccgtt ccaacctgga agaagggatc
    50641 aaacaagcgc accacttctt tcgccggaat ccccacgccg gtatcgcgaa cacggataga
    50701 gagataatcg ccatcagcgc gaacatgcaa aactatacag ccggtatcgg tgaattttat
    50761 ggcgttactc aacaggttgg agatgacctg ctgtaaacgc atcgggtcgc catttaaggc
    50821 cactggcaca tccggttcaa taaagcagta caagcctaac tgcttgcgta ccaccagcgg
    50881 taaatagttg gcggtgatgt ggttcatcac ttcacgcggt gaaaactcac gcggttcgat
    50941 cttcaactgt tccgattcaa tcttcgagaa atcgagaata tcgctgataa ttttcaacaa
    51001 caggctggaa gagttgttca ttaccgtcac cagacgatcg acgcctttcg gtaactcttt
    51061 ggtttgcaac agatccaggt taccgataat gccatacagc ggcgttcgca gctcatgact
    51121 gacggtggca aggaacatcg attttgactg gctcgcctgt tccgctgctt gtgccatctc
    51181 ctgcaacgac tcttccatct tcacgcgcga agaaacatcc accagcacac aaatggccac
    51241 gttttcatta cgatagcgcg aatggacgaa gctgatttgc agattggtat tgttgctggt
    51301 caggacatca acaaaattga cctgctgccc acagataatt tgcgtcagtc gttggcggtc
    51361 ctcatgcgta agcatattga gataggtatg cgccagttcg ttacttaaaa tattgacgcc
    51421 atcagcggta cgcaaaatgc agatacccac tggcgcggag gcgacaatct tgcgattgaa
    51481 ctgctcatgt tcttccagtc gcagggcgtc gctttccgcc ggaatgaaaa tacgtcgctc
    51541 gtacatccgc gcgagagtaa acaatgcagc tccggcaagc acattcagca aaattgcgtt
    51601 aaggatcaac atgcgaatgc gttccagcac cttatcaacc ggcaccgaat acacgatgct
    51661 tagcgatgag ggtggcagat ttttcttcag caccagctcc cggaaccctt ccgtatagcc
    51721 aaaccaggag cgttcctgca tccagcgagg atcgccctta attttacttt ctggtccggt
    51781 aagcgaaatc agggtatgac cattttcatc aagaatggta acccccatcg gcaacgtacc
    51841 cggtaagaaa aagttctcca tccggatggt ctgctcgaca cccaaaagcg cctgcaaccg
    51901 gttcgccaga taaactggcg tcaacgcgta aaaatacccg acgcaagggc gcggaccttc
    51961 gctgatccag tagaggttac tgccgctatc atcttgtggt gcatttcgat atttattgat
    52021 gcgttcatgc aacgctttca acgcggtatc gcgttccact ggcatatcac gcagaccgaa
    52081 attggccatg cagaggttat cactgccgat taaaaatacc cggttgagat cgtaagccgc
    52141 agaaaaatta tcgcgccagt agcgcataaa ccacgccaat gactccagag aacctcgcca
    52201 ggtgttactc attgcggaac aatcggagtc ggcaaacagc ggttcaaacg caggcacatc
    52261 cgcctgcgtt tctcgtccac gcggggaaag cacaccgttt tctgccgata agcgattttc
    52321 ggcgatgtac ttaagctctt tcatcacatc agaggtgcgt tgaataaagc gctgagcctg
    52381 atcggaactc agattaaatt cctgacgaat ttccgattct cgctgatgta acgcattaac
    52441 gatgtaaaaa acggatgaaa aagcaatcaa cagccagagc actaacgcca atgctctgaa
    52501 catgtagcgc gaggctttca gggttgtacg aaaagaagca aggtatttca aaggggagaa
    52561 gctccgcctc aggtgaccga tggagtgtgg ttaaggtagc ggtaaaagcg tgttaccgca
    52621 atgttctgtc accattgcaa taatgcgtat cgcttagaaa ccattgcctt aaccaggaaa
    52681 gccggagtag atggacagtt ctcagtacga aaaataagaa atgaccaatc tgccgacgct
    52741 tgttaatggt tatatcctgg cgtcggcaga tcgcccagca gtcttatctg aaaaaagaga
    52801 gagccaggtt gtgtgaaggg atggatcacc agtcaaaact tttcaccagc gtcagctcgc
    52861 cagcattacg catcggtaca ataaatgttt cctgtttctc attgaccgat ccttcatcgg
    52921 tgatcagcgt cagttgggcg gtcgtcaatt ccgtttcgct gcgcccacca tagtagttga
    52981 tatacacctg atagcgcccg tgaactggtg ctggcatggc gaaaatctcg ggtccgtacc
    53041 ccgtcgtgac atccatatcc agtgcaccac tgtttttcag cacggtgtta ccgtaccagg
    53101 cgtgttcgcc atcgggcgtg acaacatgga gatcgagatc ggtattgtcc gtatcccacg
    53161 agagaaccag ccgtaaacgt gcacgaatag ttcctgtacc cggcgttgag taaaactgca
    53221 ttttttgccg gctttgccca tccgggctga tgacctgcac gctgttgctg ccttcagtga
    53281 aaatataagg gcgtgcaaat gaaccatcgg attcaattct ctgcggcata ctggcaccgt
    53341 tgaccaccag ccggccctgc tgaactttac ccgccgcagc ataattcttt attttgccgc
    53401 ggatctgcgc tgagatattt tgatcatctg ccatgttgac ggacgacgcc gggtaattga
    53461 tcgattgtga aaaactggca tcttcgccct ctgccggatg ccagccagag agcggtgcgt
    53521 caatctcgac ttcctgcaca ccttcactgt gagcgacagg cgaaagtgcc accagtaaca
    53581 acggaagaaa aatctttcgc atagcattag tccattaata attgtcgggt cagcgtttcg
    53641 atatagttct catcaagccc ggtgggataa ccatcaaagg ccaagtgcag atactcatgg
    53701 gttaaatcca gccgatcctg aagcgtaaag aagttacgaa taaacagccg tttctgctgc
    53761 cgatcggtat aggggaaacc tgagactaaa cgacagaccg caaacacgtc tggttcgttg
    53821 taccccgtct cgccttgtaa tatacgccgc cactgcggca tttttttcgc cagccaggct
    53881 ttcgctttgg gtaataactg gcaggttgag cgtggagctc cccagcggct aaggctgttg
    53941 tcgggataag caaacgcgag tatctggtcg taacgctccc cctgccccgc ctgggccgtg
    54001 gcttgccgcc aggaaagtgt cccttcggta gcgcgactgc catgataatg aaccggatcg
    54061 cctgcataga tgagctcctg cgtccaggcg gtcatcgttc gcgcccccgt ggtcgctggc
    54121 gaagcagaaa cacgctgcgt ggcgctgctg tcggggatgg tcaggcaatc accttcccga
    54181 ttggcgtttt gctgtaaaaa agtacgtatc gccacagtca tggctttcgc tgcctcaggt
    54241 ggcgtacttt tcgcctcgcg atccagaact ctggtaacgt actcttcacg atcgagatgc
    54301 gactgcaatt tcagtttgcc cttttcgctc agtaaggtcg tctcaccgtg gctgacaaac
    54361 gtaatgtggt taccattagt aaacgtcaca cgatagcgcc cattaagtac gctgggtttt
    54421 actgccgtcg tactcttttc cgctgtgatt ttcttcagcg gatagcgggc aaacagctcc
    54481 acctctacac attgcccact ggcaacctgc gtcggcactg gcaaaacccg attaagcacc
    54541 gtcgcataac gggttagtac cgttttgctg gtgccactgc cagtaaccca cagcggtgta
    54601 ccatcggtta accagccagc aaaaccaccc tgacggattt cctgtttatc atcggcaaac
    54661 cagctccagg ttttcacccg tacccgacta ccgagcatgg atgcgacacc gattttcgcc
    54721 tcgtccagca ccacatccag taagacttcc tgagctttgt tttgcgcagg taacgtcgct
    54781 aatgaatcaa gcagcgattt aaccgttacc gaggtttctg gcttcagcgt tgtaagagac
    54841 gctaaccacg caggtgcctg acgctgttgc cagtattgcc cccacacatc tgcgccaata
    54901 tgcaaccgct gcggcgcaaa atagagccca caggaacgta ccagcgcggt atcgcgcgtt
    54961 atgctctccc cagcctgaca acaataaacc tcttccgggg agtttccgcg acactggtag
    55021 ggctgctccg gttgatgggt atcctccagc caggcgtaaa catacagttt ccacaaactc
    55081 cctaacggcg tggtgagcga gtcaggtaat gctgagactt tggtcacgcc tgatgacgac
    55141 agctgataca gttgatcgtg ctgcgcaccg cgcagcacca gttgtaacgg cgtctcctcc
    55201 gcaagcgttg gcagagtaac caacgccaat agccagacga ttctgcgcca gttcacttat
    55261 ttcacctgca tcccggtcca ttcactccct gccgcgacac tttgctgcgc aggtgcatag
    55321 gaacgcacat aacgagcagg aggcagaacg aattgccctt tttgcgagaa gcgcagcaaa
    55381 tggcggaaag tgaccgttcc cgtcagttct ttcaccggca ccatataggc cagttcgccc
    55441 atttcattac gcgctttttc cagcaattgc ccctgctgtt tcgcggcgtt gggtttattg
    55501 actgaaatgc cccatgttgt gcgctcaacg tcggctcccg gcgggagcgg tacttccacc
    55561 tgaccatagc gcagaactgc atcctgctcg ctggtaagcg taatttcatc gagatacagc
    55621 gcatcgctgt caatctcatt gctggtcacc ggctgcagag taaagctcat ctcttcttca
    55681 ccggggataa gccgatacaa ctggcgttca acggtcaccg gaatgttact ttgttgagcc
    55741 gtttttgccg gttcacgcca gcggacctgc acatttgcgg cgataattcg tcaccaaaag
    55801 agagaatgtc cggcacaccc taaccaaccc aacgccagtc ttcgccccct ccagttaact
    55861 tatgtttagc ccatgcgccc gcaggcgcag gcaaaacaac tggaggcatt gtcgccatat
    55921 atttcgccag ccagttcatg gccagcgcgc gctcaatagt ggattgctca gcggtcaaac
    55981 cattaaaata gcggccgtct gggtagcgtc accaccgcta ttgagcagca caaccgtgtg
    56041 cgccagcggc tgattgctgt ttgccgcttt atcccacgca tattgctgaa cctgtggcat
    56101 caccgtggac ttcaactgcg cttttttcag caacgtcatg gttaagacgt ttgccaccgc
    56161 atctgccagt ggagattccg gtgtatcgag gatcaggctg tcattgatat cgcggatgtc
    56221 ttcctcagag aaatcttcat ctttagttcc gcgccgggcg atagcttcat cccccccttt
    56281 caacaacgtt ttgcacggca gattcatctc ctgtgcccac gccagcacca gcgcccgatg
    56341 caataacggc atgttatcc
//
LOCUS       DAJTUN010000009        55274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTUN010000009 DAJTUN010000000
VERSION     DAJTUN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.009"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1340)
                     /gene="argH"
                     /locus_tag="OO470_000531"
     CDS             complement(<1..1340)
                     /gene="argH"
                     /locus_tag="OO470_000531"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6249889.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVA"
     gene            complement(1401..2177)
                     /gene="argB"
                     /locus_tag="OO470_000532"
     CDS             complement(1401..2177)
                     /gene="argB"
                     /locus_tag="OO470_000532"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6249890.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2185..3189)
                     /gene="argC"
                     /locus_tag="OO470_000533"
     CDS             complement(2185..3189)
                     /gene="argC"
                     /locus_tag="OO470_000533"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6249891.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3343..4494
                     /gene="argE"
                     /locus_tag="OO470_000534"
     CDS             3343..4494
                     /gene="argE"
                     /locus_tag="OO470_000534"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6249892.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4846..7497
                     /gene="ppc"
                     /locus_tag="OO470_000535"
     CDS             4846..7497
                     /gene="ppc"
                     /locus_tag="OO470_000535"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6249893.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7680..9413
                     /locus_tag="OO470_000536"
     CDS             7680..9413
                     /locus_tag="OO470_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6249894.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9562..10413
                     /locus_tag="OO470_000537"
     CDS             9562..10413
                     /locus_tag="OO470_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6249895.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10400..10741)
                     /locus_tag="OO470_000538"
     CDS             complement(10400..10741)
                     /locus_tag="OO470_000538"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6249896.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10743..11621)
                     /locus_tag="OO470_000539"
     CDS             complement(10743..11621)
                     /locus_tag="OO470_000539"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6249897.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11587..13884)
                     /locus_tag="OO470_000540"
     CDS             complement(11587..13884)
                     /locus_tag="OO470_000540"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6249898.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(13935..14255)
                     /locus_tag="OO470_000541"
     CDS             complement(13935..14255)
                     /locus_tag="OO470_000541"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6249899.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14270..15349)
                     /locus_tag="OO470_000542"
     CDS             complement(14270..15349)
                     /locus_tag="OO470_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6249900.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15658..16761
                     /locus_tag="OO470_000543"
     CDS             15658..16761
                     /locus_tag="OO470_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6249901.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16762..18159
                     /gene="ptsP"
                     /locus_tag="OO470_000544"
     CDS             16762..18159
                     /gene="ptsP"
                     /locus_tag="OO470_000544"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6249902.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18171..18833
                     /gene="fsa"
                     /locus_tag="OO470_000545"
                     /pseudo
     CDS             18171..18833
                     /gene="fsa"
                     /locus_tag="OO470_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18844..19945
                     /gene="gldA"
                     /locus_tag="OO470_000546"
                     /pseudo
     CDS             18844..19945
                     /gene="gldA"
                     /locus_tag="OO470_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20220..20837
                     /locus_tag="OO470_000547"
     CDS             20220..20837
                     /locus_tag="OO470_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6249903.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20864..21769)
                     /gene="yijE"
                     /locus_tag="OO470_000548"
     CDS             complement(20864..21769)
                     /gene="yijE"
                     /locus_tag="OO470_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6249904.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21863..24043)
                     /gene="katG"
                     /locus_tag="OO470_000549"
     CDS             complement(21863..24043)
                     /gene="katG"
                     /locus_tag="OO470_000549"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6249905.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24372..25262)
                     /gene="metF"
                     /locus_tag="OO470_000550"
     CDS             complement(24372..25262)
                     /gene="metF"
                     /locus_tag="OO470_000550"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6249906.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25611..28043)
                     /gene="metL"
                     /locus_tag="OO470_000551"
     CDS             complement(25611..28043)
                     /gene="metL"
                     /locus_tag="OO470_000551"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6249907.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28046..29206)
                     /gene="metB"
                     /locus_tag="OO470_000552"
     CDS             complement(28046..29206)
                     /gene="metB"
                     /locus_tag="OO470_000552"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6249908.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29483..29800
                     /gene="metJ"
                     /locus_tag="OO470_000553"
     CDS             29483..29800
                     /gene="metJ"
                     /locus_tag="OO470_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6249909.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            29984..30592
                     /locus_tag="OO470_000554"
     CDS             29984..30592
                     /locus_tag="OO470_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6249910.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30653..30865)
                     /gene="rpmE"
                     /locus_tag="OO470_000555"
     CDS             complement(30653..30865)
                     /gene="rpmE"
                     /locus_tag="OO470_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6249911.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31068..33266
                     /gene="priA"
                     /locus_tag="OO470_000556"
     CDS             31068..33266
                     /gene="priA"
                     /locus_tag="OO470_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6249912.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33422..34447
                     /gene="cytR"
                     /locus_tag="OO470_000557"
     CDS             33422..34447
                     /gene="cytR"
                     /locus_tag="OO470_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6249913.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34539..35498
                     /gene="ftsN"
                     /locus_tag="OO470_000558"
     CDS             34539..35498
                     /gene="ftsN"
                     /locus_tag="OO470_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6249914.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35591..36121
                     /gene="hslV"
                     /locus_tag="OO470_000559"
     CDS             35591..36121
                     /gene="hslV"
                     /locus_tag="OO470_000559"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6249915.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36131..37462
                     /gene="hslU"
                     /locus_tag="OO470_000560"
     CDS             36131..37462
                     /gene="hslU"
                     /locus_tag="OO470_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6249916.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37529..38455
                     /gene="menA"
                     /locus_tag="OO470_000561"
     CDS             37529..38455
                     /gene="menA"
                     /locus_tag="OO470_000561"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6249917.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38548..39033
                     /gene="rraA"
                     /locus_tag="OO470_000562"
     CDS             38548..39033
                     /gene="rraA"
                     /locus_tag="OO470_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6249918.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39118..39363)
                     /gene="zapB"
                     /locus_tag="OO470_000563"
     CDS             complement(39118..39363)
                     /gene="zapB"
                     /locus_tag="OO470_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6249919.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39789..40634
                     /gene="glpF"
                     /locus_tag="OO470_000564"
                     /pseudo
     CDS             39789..40634
                     /gene="glpF"
                     /locus_tag="OO470_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40674..42164
                     /gene="glpK"
                     /locus_tag="OO470_000565"
     CDS             40674..42164
                     /gene="glpK"
                     /locus_tag="OO470_000565"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6249920.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42394..43404
                     /gene="glpX"
                     /locus_tag="OO470_000566"
     CDS             42394..43404
                     /gene="glpX"
                     /locus_tag="OO470_000566"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6249921.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43501..44247
                     /gene="fpr"
                     /locus_tag="OO470_000567"
     CDS             43501..44247
                     /gene="fpr"
                     /locus_tag="OO470_000567"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6249922.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44252..44680)
                     /gene="uspD"
                     /locus_tag="OO470_000568"
     CDS             complement(44252..44680)
                     /gene="uspD"
                     /locus_tag="OO470_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6249923.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44707..45006)
                     /locus_tag="OO470_000569"
     CDS             complement(44707..45006)
                     /locus_tag="OO470_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6249924.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45218..45658)
                     /locus_tag="OO470_000570"
                     /pseudo
     CDS             complement(45218..45658)
                     /locus_tag="OO470_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45759..46358
                     /locus_tag="OO470_000571"
     CDS             45759..46358
                     /locus_tag="OO470_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6249925.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46466..47233
                     /gene="tpiA"
                     /locus_tag="OO470_000572"
     CDS             46466..47233
                     /gene="tpiA"
                     /locus_tag="OO470_000572"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6249926.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47288..48043)
                     /gene="cdh"
                     /locus_tag="OO470_000573"
     CDS             complement(47288..48043)
                     /gene="cdh"
                     /locus_tag="OO470_000573"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6249927.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48150..49139)
                     /gene="sbp"
                     /locus_tag="OO470_000574"
     CDS             complement(48150..49139)
                     /gene="sbp"
                     /locus_tag="OO470_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6249928.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49459..50421)
                     /gene="pfkA"
                     /locus_tag="OO470_000575"
     CDS             complement(49459..50421)
                     /gene="pfkA"
                     /locus_tag="OO470_000575"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6249929.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50602..51504)
                     /gene="fieF"
                     /locus_tag="OO470_000576"
     CDS             complement(50602..51504)
                     /gene="fieF"
                     /locus_tag="OO470_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6249930.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51652..52152)
                     /gene="cpxP"
                     /locus_tag="OO470_000577"
     CDS             complement(51652..52152)
                     /gene="cpxP"
                     /locus_tag="OO470_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6249931.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52302..53000
                     /gene="cpxR"
                     /locus_tag="OO470_000578"
     CDS             52302..53000
                     /gene="cpxR"
                     /locus_tag="OO470_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6249932.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            52997..54370
                     /gene="cpxA"
                     /locus_tag="OO470_000579"
     CDS             52997..54370
                     /gene="cpxA"
                     /locus_tag="OO470_000579"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6249933.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54476..55150)
                     /gene="yiiM"
                     /locus_tag="OO470_000580"
     CDS             complement(54476..55150)
                     /gene="yiiM"
                     /locus_tag="OO470_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6249934.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13463 a        14980 c        14123 g        12708 t
ORIGIN      
        1 tgcgccacct gctgcggtga gacgccgcct tttgccgcac gcttttcgag gcacgattgt
       61 agcgacagaa tcggatagac atcttcgcca atcacctgac tgaatttctg caactcactg
      121 agtggcagat cttccagcgg tttgccctga cgaatggctt ccaccaccgc ttcacccaca
      181 atatggtgcg cctcgcggaa aggtacgcct ttcgccacca ggtaatccgc cagttcagtg
      241 gcgttcgcat acccctgctg cgccgcttcc tgacaacgcg gacgtttcac ctgaatgccg
      301 tccagtacca gcgccgccat atgcaggcag tccagccagg tatcgagcgc gtcgaacaga
      361 ccttctttgt cttcctgcat atctttgttg taagccagcg gcaaaccttt cagcgtcatc
      421 atcatgccgg ttaacgcacc ctgcacccgg ccgcatttac cgcgaatcag ctccagcgca
      481 tccgggtttt tcttctgcgg cattaatgat gaaccggaag tcacgcggtc cgaaagctcc
      541 acaaaccccg cttcgccggt gttaaagaaa atcagatctt cagcaaaacg cgacagatgc
      601 accatgccga tagcggcagc agaaagcagt tccaacacat ggtcacggtc agaaacgctg
      661 tccagactgt tacgggtcgc cgaagcaaag cccagccagc ctgctaactg ttcacggtcg
      721 atttcatagg ccgttcccgc cagcgcgcca cagcctaatg ggctgacatc cagacgctta
      781 agcgcatcct gcaaacggct ttcatcacgc gccagcatct caacataggc caggcaccag
      841 tgcgcgaacg tcaccggctg ggcgcgttgc aggtgagtgt aacctggcat taccgcgtcc
      901 tgattgtttt gtgcggtttc caccagcgcc gattgcagct gccggttagc cgtcagtaac
      961 tcgctaacgg tatctttgca ccacagtttc aggtcagtcg ctacctgatc attacggcta
     1021 cgcccggtat gcagcttttt gcctaactgg cccactttgt cgatcagttt gccttccacc
     1081 cagctatgga tatcttcggc gtcgctttca aggatttgtt gtggcctggc gcgaacatct
     1141 tccagcaaca cgttcagcgc ctcttccagt tgcgcctgct cttctgcagt taacacgccg
     1201 accgttacca aggctttgga ccaggccaca gagccaacaa tatcctgctc cgccagacgg
     1261 taatcaaagc gcagtgagtc gttgaattgt ttgaaccgtt gatctgctgc ctgggtaaaa
     1321 cgcccgcccc aaagtgccat aactctgttt ccttattttg aaattcaatg ccggaaagct
     1381 gcgcctccgg ctaacaaaac ttaagctaaa atccgcgtac ccatcggcat accgttaaac
     1441 agtgccggaa gctgctccgc ataacgccaa gaagcgatat ctaccggacg gcccagcgtg
     1501 cgggccgcat ccagcgccgc gttcactttc actatcatgc cgtcagtaat aatgccctgc
     1561 tcaatcagtt gttctgcttt cgcggcggtc atttcggcaa tgcgttgccc tttgccgtcg
     1621 agaatgccgc tgacgtcgga gagcaaaatc agatccgcgc ccagcgttgc cgccagcgcc
     1681 gttgccgcct ggtcggcatt gacgttcatc agttgccctt cgtctgttac gccaatggag
     1741 ctgaccaccg gcagataacc gttctccagc aaggagttga taagcttagg cgaacctggc
     1801 tgcgccagtc caacatgacc taactcttca tcaagctggg tcactttgac gctgtcgccg
     1861 tcaccgagaa acaaacctac ggccgcaatc tgatgtttct tcgcccatgc caacagggtt
     1921 ttatttgccg ttcccgccag tgctccggtg ataatgtcta tctgatcagc aggcgtcacc
     1981 cgcaggccgt ttttcttttt caccggcaga ttcagccctt tcatcagctc atccaccacg
     2041 caaccgccgc cgtgcacaat caccagcgga cgctgatgtg actcacgata attcaccaat
     2101 gcgctaaaca gacgttccag cgcctcttca ctatccagca gtacgccgcc cagtttgata
     2161 attaatggat tcatcattgc acccttaaat aagagactgc gtttccgcat agccgaaacg
     2221 aatattggcg cactgtaccg cttgtgccgc cgcgcctttc agtaagttgt cttcggtcgc
     2281 cacaataatc agatgctcgc cctgaacggc aaacccgata tcgcaaaatg gcagcccaac
     2341 gacatttttc agcgccggaa cgcctttgtc atacagccgc accagcggtt tatgggcata
     2401 cgcctgctgt aacgcttgcg cgacttgcgc ctgggtcaca cccgatttca ggcggcaggt
     2461 aatggtttcg agaatgccgc gcgggaaatt gcccagatgt ggggtgaaga taacgtcagc
     2521 accgaggtgt gtggcgatct ctggttgatg gcgatgagta aagacgccat acggttgcag
     2581 gctaacttca caaaaactat ttgaaatcgc tgccttacgt cctgcaccgc tcacgccgct
     2641 ggtggcgttg atcaccggcc actgattgag gtcaagaaga tcggcatcaa tcaacggttt
     2701 cagcgccagc tgtgccgccg tcggataaca gcctggcacc gcaatcaaat tcgcttcttt
     2761 taatttatta ccgcaccact ccgccagacc gtaggctgcc tgttccagca gttccgggta
     2821 ttgatgggta aagccgtaat atttttcata gaaggtggcg tcgttaacac gaaacgcgcc
     2881 ggaaaggtcg aacaccacgc agcccgcttc aagaaactgc ggcgctaaat cgtggctaac
     2941 ttcatgggcg gtggcgagaa acactacgtc cacccctggg ctaaactcgc tgatatccga
     3001 catcggctgc aacggcagct caacgatgcc ttttagctgc ggatgcaaat cggagattaa
     3061 ctttcccgca tcattgcttt gcgctgaaac agtcaaagcg gttatgttca tatgcggatg
     3121 gcgatttaca taggtcacta gctctgcgcc agcgtagccg ctggcaccca caatcagcgt
     3181 attcaacatc ggggctattc accttcttat gtctggttgc caggttaaac gtaaaacatt
     3241 caccttacgg ctggtgggtt ttattacgct caacgttagt gtatttttat tcataaatac
     3301 tgcatgaata ttgatactat catgaccaga ggtgtgtcaa caatgaaaaa caaattaccg
     3361 ccatttatcg agatttaccg cgctctgatt gccacacctt caataagcgc tacggaagag
     3421 gcactcgatc aaagcaatgc agatttaatc actctgctgg cggactggtt taaagatttg
     3481 ggcttcaatg tggaagtgca gcctgttcca ggaactcgca acaaatttaa tatgctggcc
     3541 agttacggac agggggctgg cggcttgttg ctggcggggc ataccgatac ggtgccattt
     3601 gatgacggtc gctggacgcg cgatccgttt acactgacgg agcatgacgg caagctttac
     3661 ggcttaggca ccgccgacat gaaaggcttt tttgcgttta tccttgatgc gctacgcgat
     3721 gtcgacgtca cgaaactgaa aaaaccgctc tacattctgg cgactgctga tgaagaaacc
     3781 agtatggccg gagcgcgtta ttttgccgaa actaccgccc tgcgcccgga ttgcgccatc
     3841 attggcgaac cgacgtcact acaaccggta cgcgcacata aaggtcatat ctctaacgcc
     3901 atccgtattc agggccagtc ggggcactcc agcgatccag cacgcggaat taacgctatc
     3961 gaactaatgc acgacgccat cgggcatatt ttgcaattgc gcgataacct gaaagaacgt
     4021 tatcactacg aagcatttac cgtgccatac cctacgctca acctcgggca tattcacggt
     4081 ggcgacgctt ctaaccgtat ttgcgcttgc tgtgagttgc atatggatat tcgtccgcag
     4141 cctggcatga cactcaatga acttaatggt ttgctcaacg atgcattggc tccggtgagc
     4201 gaacgctggc cgggtcgtct gacggtcgac gagctgcatc cgccgatccc tggctatgaa
     4261 tgcccaccga atcatcaact ggttgaagtg gttgagaaat tgctcggagc aaaaaccgaa
     4321 gtggtgaatt actgtaccga agcgccgttt attcaaacgt tatgcccgac gctggtgctc
     4381 ggtccgggtt ccatcaatca agctcatcag cctgatgaat atctggagac gcggtttatt
     4441 aaacccaccc gtgagttgat tatccaggta attcaccatt tttgctggca ttaaaacgta
     4501 ggccggataa ggcgctcgcg ccgcatccgg caccgttgcc aaactccggt gccgcaataa
     4561 tgtcggatgc gatgcttgcg catcttatcc gacctacacc tttggtgtta tgtatggcga
     4621 ttttttaaca tttccataag ttacgcttat ttaaagcgtc gtgaatttaa tggcgtaaat
     4681 tcctgctatt tattcgtttg ctgaagcgat ttcgcagcat ttgacgtcac cgcttttacg
     4741 tggctttata aaagacgacg aaaagcaaag cccgagcata ttcgcgccaa tgcgacgtga
     4801 aggatacagg gctatcaaac gataagatgg ggtgtctggg gtaatatgaa cgaacaatat
     4861 tccgcattgc gtagtaatgt cagtatgctc ggcaaagtgc tgggagaaac catcaaggat
     4921 gcgttgggag aacacattct tgaacgcgta gaaactatcc gtaagttgtc gaaatcttca
     4981 cgcgctggca atgatgctaa ccgccaggag ttgctcacca ccttacaaaa tttgtcgaac
     5041 gacgagctgc tgcccgttgc gcgtgcgttt agtcagttcc tgaacctggc caacaccgcc
     5101 gagcaatacc acagcatttc gccgaaaggc gaagctgcca gcaacccgga agtgatcgcc
     5161 cgcaccctgc gtaaactgaa aaaccagccg gaactgagcg aagacaccat caaaaaagca
     5221 gtggaatcgc tgtcgctgga actggtcctc acggctcacc caaccgaaat tacccgtcgt
     5281 acactgatcc acaaaatggt ggaagtgaac gcctgtttaa aacagctcga taacaaagat
     5341 atcgctgact acgaacgcaa ccagctgatg cgtcgcctgc gccagttgat cgcccagtca
     5401 tggcataccg atgaaatccg taagctgcgt ccaagcccgg tagatgaagc caaatggggc
     5461 tttgccgtag tagaaaacag cctgtggcaa ggcgtaccga attacctgcg cgaactgaac
     5521 gaacaactgg aagagaatct cggctacaaa ctgcccgtcg aatttgttcc ggtacgtttt
     5581 acctcgtgga tgggcggtga ccgcgacggc aacccgaacg tcactgccga tatcacccgc
     5641 cacgtcctgc tgctcagccg ctggaaagcc accgatctgt tcctgaaaga tattcaggtt
     5701 ctggtttctg aactgtcgat ggttgaagcg acccctgaac tgctggcgct ggttggcgaa
     5761 gaaggtgccg cagaaccgta tcgctatctg atgaaaaacc tgcgttctcg cctgatggcg
     5821 acacaggcat ggctggaagc gcgcctgaaa ggcgaagaac tgccaaaacc agaagggctg
     5881 ctgacacaaa acgaagaact gtgggaaccg ctctacgctt gctaccagtc acttcaggcg
     5941 tgtggcatgg gtattatcgc caacggcgat ctgctcgaca ccctgcgccg cgtgaaatgt
     6001 ttcggcgtac cgctggtccg tattgatatc cgtcaggaga gcacccgcca taccgaagcg
     6061 ctgggcgagc tgacccgcta cctcggtatc ggcgactacg aaagctggtc agaggccgac
     6121 aaacaggcgt tcctgatccg cgaactgaac tccaaacgtc cgcttctgcc gcgcaactgg
     6181 caaccaagcg ccgaaacgcg cgaagtgctc gatacctgcc aggtgattgc cgaagcgccg
     6241 caaggctcca ttgccgccta cgtgatctcg atggcgaaaa cgccgtccga cgtactggct
     6301 gtccacctgc tgctgaaaga agcgggtatc gggtttgcga tgccggttgc tccgctgttt
     6361 gaaaccctcg atgacctgaa caacgccaac gatgtcatga cccagctgct caatatcgac
     6421 tggtatcgcg gcctgattca gggcaaacag atggtgatga ttggctattc cgactcagca
     6481 aaagatgcgg gcgtgatggc agcttcctgg gcgcaatatc aggcacagga tgcattaatc
     6541 aaaacctgcg aaaaagcggg tattgagctg acgttgttcc acggtcgcgg cggttccatt
     6601 ggtcgcggcg gcgcacctgc ccatgcggcg ctgctgtcac aaccgccagg aagcctgaaa
     6661 ggtggcctgc gcgtgactga acagggcgag atgatccgct ttaaatatgg tctgccagaa
     6721 atcaccgtca gcagcctgtc gctttacacc ggggcgattc tggaagccaa cctgctgcca
     6781 ccacctgagc cgaaagagag ctggcgtcgc attatggatg aactgtcagt catctcctgc
     6841 gatctctacc gtggctacgt acgtgaaaac aaagattttg tgccttactt ccgctccgct
     6901 acgccggaac aagaattggg caaactgccg ttgggttcac gtccggcgaa acgtcgccca
     6961 accggcggcg tcgagtcgct gcgcgccata ccgtggattt ttgcctggac gcaaaaccgc
     7021 ctgatgctcc ccgcctggct gggtgcaggt acggcgctgc aaaaagtggt cgaagatggc
     7081 aaacagagcg agctggaagc catgtgccgc gattggccat tcttctcgac gcgtctcggc
     7141 atgctggaga tggtcttcgc caaagcagac ctgtggttgg cggaatacta tgatcaacgt
     7201 ctggtagaca aagcactgtg gccgttaggt aaagagttac gcaacctgca agaagaagac
     7261 atcaaagtgg tgctggcgat tgccaacgat tcccatctga tggccgatct gccgtggatt
     7321 gcagagtcta ttcagctacg gaatatttac accgacccgc tgaacgtatt gcaggccgag
     7381 ttgctgcacc gctcccgcca ggcagaaaaa gaaggccagg aaccggatcc tcgcgtcgaa
     7441 caagcgttaa tggtcactat tgccgggatt gcggcaggta tgcgtaatac cggctaatct
     7501 tcctcttctg caaaccctcg tgcttttgcg cgagggtttt ctgaaatact tctgttctaa
     7561 caccctcgtt ttcaatatat ttctgtctgc attttattca aattctgaat ataccttcag
     7621 atatccttaa ggaattgtcg ttacattcgg cgatattttt ttcaagacag gttcttacta
     7681 tgcattccac agaagtccag gctaaacccc tttctagctg gaaagccctg ggttgggcac
     7741 tgctctactt ttggtttttc tctactctgc tacaggccat tatttacatc agtggttata
     7801 gtggcaccaa cggcattcgc gactcgctgt tattcagctc gctgtggttg atcccggtat
     7861 tcctctttcc gaagcggatt aaaattattg ccgcagtgat cggcgtggtg ctatggacgg
     7921 cctctctggc ggcgctgtgc tactacgtca tctacgggca ggagttctcg cagagcgttc
     7981 tgtttgtgat gttcgaaacc aacaccaacg aagccagcga gtatttaagc cagtatttca
     8041 gcctgaaaat tgtgcttatc gcgctggcct atacggcggt ggcagttctg ctgtggacac
     8101 gcctgcgccc ggtctatatt ccaaagccgt ggcgttatgt tgtctctttt gccctgcttt
     8161 atggcttgat tctgcatccg atcgccatga atacgtttat caaaaacaag ccgtttgaga
     8221 aaacgttgga taacctagcc tcgcgtatgg agcctgccgc accgtggcaa ttcctgaccg
     8281 gctattatca gtatcgtcag caactaaact cgctaacaaa gttactgaat gaaaataatg
     8341 ccttgccgcc actggctaat ttcaaagatg aatcgggtaa cgaaccgcgc actttagtgc
     8401 tggtgattgg cgagtcgacc cagcgcggac gcatgagtct gtacggttat ccgcgtgaaa
     8461 ccacgccgga gctggatgcg ctgcataaaa ccgatccgaa tctgaccgtg tttaataacg
     8521 tagttacgtc tcgtccgtac accattgaaa tcctgcaaca ggcgctgacc tttgccaatg
     8581 aaaagaaccc ggatctgtat ctgacgcagc cgtcgctgat gaacatgatg aaacaggcgg
     8641 gttataaaac cttctggatc accaaccagc agacgatgac cgcccgcaat accatgctga
     8701 cggtattttc gcgccagacc gacaagcagt actacatgaa ccagcaacgt acgcagagtg
     8761 cgcgtgaata cgacaccaac gtgctgaagc cgttccagga tgtgctgaat gaccctgcgc
     8821 cgaagaaact gatcatcgtt catctgctgg gtacgcatat caaatacaaa taccgctacc
     8881 cggaaaatca gggcaagttt gatggcaata ccgatcatgt tccgccagga ttaagcgcag
     8941 aagagctgga atcatataac gattatgaca acgctaacct gtataacgat catgtggttg
     9001 ccagcctgat taaagacttt aaagcggcag acccgaacgg attcctggtt tacttctctg
     9061 accacggtga agaggtttac gacacgccgc cgcacaaaac tcaggggcgt aacgaagaca
     9121 acccgacgcg ccacatgtac accattccgt tcctgctgtg gacgtcggaa aaatggcaag
     9181 cgactcatcc ccgtgatttc tcacaggatg tcgatcgtaa atacagcctg gcggaactga
     9241 tccacacctg gtcagatttg gcgggcttat cttacgacgg ttacgatcca acccgttcag
     9301 tggtgaatcc gcagttcaaa gaaactaccc gctggattgg taacccgtac aagaaaaacg
     9361 cgctgatcga ttacgacact ctgccgtatg gcgatcaggt gggtaatcag tagatgacct
     9421 ttgtatcgcg tctggcgcta tttgccggat gcgatactgc cgcaatgttt acatcgaact
     9481 cccctcaggc cacacttttc aggtggcctt ttttaataat gccagttact gttttcccgt
     9541 ctacatacct aagggaataa catgtatcac gacgtcagct atctgctctc ccgcctgatc
     9601 aacggcccgc tgtccctgcg ccagatttac tttgccagca gtaacggccc ggtgcccgat
     9661 cttgcgtatc aggtcgattt tccacggctg gaaattgtgc tggaaggtga gtttgttgat
     9721 accggcgctg gagcaacgtt agttcccggc gatgtgctgt acgttgccgc tggtggctgg
     9781 aatttcccac aatggaaaac acccgctacc acctttagcg tgctgtttgg caaacagcaa
     9841 ctcggcttca gcgtcgtgca atgggatggc aaacaatatc aaaatctggc gaagcaacac
     9901 gtcgcccgac gcggcccacg cataggttct tttctgctac aaacgctcaa tgaaatgcaa
     9961 atgcagccgc aggagcagca aacggcaagg cttatcgtag ccagcctgct tagccactgc
    10021 cgcgatttgc tcggcagcca aatccagacc gcctcacgca gccaggcact atttgaagct
    10081 attcgcgatt atatcgacga acgctatgcc tccgcgctta cccgcgaatc tgtcgcacag
    10141 gcattctata tttcaccaaa ctatctctcg cacctgtttc aaaaaacggg ggccattggt
    10201 tttaacgaat acctgaatca cacgcgactg gagcacgcta agacgttatt gaaaggctat
    10261 gacctgaagg taaaagaagt ggcgcacgcc tgcggctttg tcgacagcaa ctacttctgc
    10321 cgtctgtttc gtaaaaacac cgaacactcg ccgtcggaat atcgccggca gtaccacagc
    10381 caactgacag aaaaaccgac tactccagaa taagatgggt ttgctgcggc gcagaaagca
    10441 ctttgcgcac cgcgctcatt acccgctgcg gctcacgcag gaatgcgtag atgctgcatt
    10501 gcacatagcg gcaatgttca aatcgctcgg caccggcaag ctcgatatcc gtaatcaaca
    10561 gagcaacatc cgcccgacgg atatcctcgt ccgctaaacg attctccgtt cccagcgcgc
    10621 cctgagtttc aatgctgacg ccccacttct ctaactggca caacttttcc agccgttccg
    10681 ccgccatata agtatgcgcc acgccgctta cgcaggcggt tactgccacc aggtatgcca
    10741 ttttaacctc ccacggtaac ctgaaatccg gcccgttctg ccatttcgcg catcgttgtc
    10801 acatcggctg acgacggcgc aggcacctct ttcatcgacc atgttttccc cagcaggcgg
    10861 tatttcggtt cgccgtactg atgaaacggt aacagatgga tctgcctgat tttcagcggg
    10921 atcagtacat ccagcgcctg ctgcatattc tcccggctga gcgtgaaacc agggatcaac
    10981 ggtaaacgcg ggatcacgtt gacaccctca ctcaccagca aacgcagatt ctccagcacg
    11041 cgtggcaggt tcatcttcac cacatcccgc gcctgagtcg cgtccataat ttttaaatcg
    11101 aacagcactt catcgcacaa tttcgccagc ggtaacagct tgcttgctgg cgcgtctccg
    11161 gcagtttcaa tggcgcatga gacaccccac agccgcagtc gctgtaaaaa acgggtagca
    11221 aactccgcct gcattaacac ttcgccgcca gaaagcgtga cgccgccgcc ggacgtgcga
    11281 aagaaaatgt catctttcat cacttcccgt tccagagcgt caaggctgat atcgcgacca
    11341 atccgttcaa acgccccgga ggggcattca tccgcatcac gcaaacattt cgcacagtgc
    11401 agacatttcg cctctctgcg taccgtctgg attttgccgg agatcgactc cggattagca
    11461 caccacgggc aaagatgcgg acagccttta aaaaagacca ccgtacgaat gccctcgccg
    11521 tcattcagtg aataacgctg aatgttgaaa atgcgcgcca catcatcgcg gcgcgtttcc
    11581 acaacgttac agctgatgcg ctgtccggcg gatgatgtca tcctggatct ccttcgacag
    11641 ttcgacaaag aaggcgctgt atccggcaac gcgcaccacc agcccggcat aatcttgtgg
    11701 gcgctgttgc gcttcccgca acgtgtcggc gttcaccacg ttaaactgaa tatgctgcaa
    11761 cttaagctgg gtaaacaccc gtaagaagtc ggccagtttg cgtaatcccg cttcaccttc
    11821 cagggtcgcc ggagtgaatt tcacgttcag caacgtaccg ttagacagca gcgtattatc
    11881 gagcttactg actgacttca gtaccgccgt tggcccttgt gcgtcctgac ccagcatagg
    11941 tgacaagccg ccgtctgcca gttgttcccc ggcaaaacga ccgtctggcg tcgcgccaac
    12001 caccgatccc aacggaacgt gagcagaaac ggtatacgat cccggcgtga agtaaccgcc
    12061 gcgcgggttc tggtattttt ccacttcttt gcagtagtgg cgcaacagtt cggcgctaat
    12121 gttatccacc tcgtcgatat cgttaccgta tttctcaaag cggttaatta agcgagcgcg
    12181 gactttttcg ccttctggcg ttgcaaagtt ggcttttaat accgacagca attcgtcaaa
    12241 acttaaacgc tgttgctcaa aaaccatccc tttcaacgca tggagagaat cgctcaggtt
    12301 ggcgatacca attccctgta cgccggagaa gttataacgc gcgccgccat cggtaatatc
    12361 gcgacctttt tccagacaat cgctgataaa agacgagagc agcggtacag gtgcccagtc
    12421 gcgatggccg atatcacaaa tattgctgcc ctcaaccatc agggtgatgt agtggctgat
    12481 cttggcgcgg atctgctcca gtaaaccttc ataagtcagc gcagcattgc cttcattttc
    12541 atgcaggcag atttccatca ctttcagcag attaaacatc gcgatgtcat gcaagccgta
    12601 ggttctgccg ggaatagata attccacaca gcccactacg gaatagtcgc gcgcatcttc
    12661 cagcgaaacg ccacggttga ggaacgctgg caccaccact tcatcgttaa agatttgcgg
    12721 aataccggtg ccgaggcgaa tggtttcggc ggttttcatc aggaacggcg tgtcgatcaa
    12781 ggcgttagtg cgcacgccga ggttcggttg cggtaattgc acgctttgat aggtgtcaag
    12841 gcaaaggaac gaaagcacgt tcaccgcgct acgtccgttc tcggttaacc cgccgagcag
    12901 tgcggtatag ccggtcggga aaccggcgaa ataacgcgcg ctactggtgg agcgcaacag
    12961 cacgatgtcg ttgcatttca cccataaaga ttcgagcagt tctttcagga acgccggatc
    13021 ttcgccctgg gttaaagatg cctgatagaa cggcaacata tactggtcga agcgccctaa
    13081 agacagcgaa ctggcgttgg attcgtattg cagaatgatg ttcatgtacc agaataactg
    13141 gcacgcctgc cagaacgtct gcggcttatg ttgcgcgtta tggcgggaga tctccgcaat
    13201 agtcagcagc tcttcgcgac gctgggcatc tgtgcagttt gccgccatcg tttccgccag
    13261 ttcggcgtaa cgcaaaatgt gtttctgcga ggcttccagc agtaacaacg cggcctgata
    13321 aaagtgattc tccggctgtt gctgacaatg ttgctgcatc tgtgccacca gctcacccag
    13381 accgtgattc aacaggcgtg ggtaatcaat aataatgtgc ccctggcctt tatccgtctg
    13441 gttgatgctg aaaatctgcg tgttggtcgc ggcttttact tcatctgtca tctgcccgtt
    13501 gatgaaatct ttcatcgaac gtttttccca gtacgggaac aactcttcac ggtagatacg
    13561 tttatcttct tcgctgatag caaagcggtc ctgcggacgc gtcgggaatt gatccagctc
    13621 tttcagcagc cagtaagggt ccatttccgg cgacataatc ccggcgcgcg gttttacggt
    13681 gcggttaccg gcaatcagtt cttcatcacg aatcgaaatt tcaacatgtt caaggatata
    13741 cgctgttgct ttcgcccggc gcaatatcac cggttcgcct tcggtttgcc gatggctggc
    13801 ggtataaagc agcgcccgct ccagcgagat ttcacgggta ttggcaaaca gtgcagtttt
    13861 gaggcgagag atacgattcg tcatgaggaa ctcctgtggg aaagcgaacc cgcccttgcg
    13921 agcgggagaa gaacttaagc ggtttgcgcc aggtgagctt caattttgct cataatggcg
    13981 tcagcacgtt ttactgcatc gctgatgttg acgcgcacga tggtcttgcc agcaaaacgc
    14041 tcttcaaact tgatgccgat atctttggtc agaatcacca tatcagcgct cgccacgtct
    14101 tccgcagtca gttcattttc cagaccaatt gacccctggg tttctacttt cacttcccag
    14161 cctttcgctt tcgcggcact ttccagcgcc tctgctgcca tataggtatg ggcaacacct
    14221 gaaggacatg cggttactgc aataattttc gtcatggctc gttcctcaat taattaattt
    14281 caaaatccaa atccaggtcg tcttcttttt catcagtcga agacccattt ttacgcgcca
    14341 gacttttcag cacgttcaca caaacagccg tcaccactgc tccgacagcc actgctgcga
    14401 tataacccaa cttaccttca accaccggca gcacaatcag accaccccag cctgcatagc
    14461 actgcgcacc gaccagcgcc gcagttactg caccacaaac tgaaccgacc ataatggaag
    14521 gaatgacacg cagcggatcg gcagcggcga aaggaatcgc cccttcagta acgccaacac
    14581 agcccatcac cagtgccgct ttgccggttt cgcgctcttc tgcggagaaa tttttacgac
    14641 caatcagcgt cgccagcccc atccccagcg gcgggataca aatacccacg gcagcaatgg
    14701 ccaccacggt ataaacaccc tgagcaacgc aaatcagcat gaaggcatag gccactttgt
    14761 taaccggacc gcccatatcg aacgccagca tcagccccat gatcaccgcc agcataacaa
    14821 tgctgccctg ctgcatcccc tgaagccact gagtcaggct gttggtcaac gcccctacag
    14881 gctcgcccaa gccccacatc atgatgcctg cggtaatcag tgtgccgatg ataggaataa
    14941 tgaagatggg catcaccgag cgcagaactt tatgcaccgg aattttcttc aggtaatgca
    15001 ccacgatgcc gccgataatc ccggcgatca gcgcaccaaa gaagcccgca ccaaagctgt
    15061 taccaaccca ggcaccgata gcgcacggag ccagcgcaga acgctctgca atggagtaac
    15121 caatgtaagc agcgaggaaa ggcaccatca gcgtcaaacc tgcaacgccg atatcaaaca
    15181 gttttttcaa attcggatcg gctacggcat ccggcactgc gcctttgcca tacaacatga
    15241 cggaaaccgc cagcaaaata ccgcccgata ccacgaaggg aatcatgtgt gaaacgcccg
    15301 tcattaaatg ctgacgggta tttttcagga tctgcaccaa ctcattcata agctgctccc
    15361 gggcttgcaa aagcccatgt ccatattttt gatggaggaa acaataggca atcgcaggcg
    15421 gagcaaacag tggataaaaa ggccatttac tggacttttg taatatcagt acaaaaatgc
    15481 gatccgcctc ataacttgcg ataatccgga agcatgccgc aaaacggcgt cttgtgagac
    15541 gcgtcgcaaa ttgacggtta cattacattt gtccagcttt tggcagattt gtcacatggc
    15601 aggccgttac cgtgcgctct tatcctgtaa acagaaaacc gtaccgggag aaaggcaatg
    15661 gccctgattg tggaatttat ttgtgagcta cctaacggcg tacatgcgcg tccggcaagc
    15721 cacgttgaaa cgctgtgtaa tactttttca tctcaaattg agtggcataa cctgcgtact
    15781 gaccgtaaag gcaacgccaa aagcgctctg gcgctgattg gcaccgatac gctggtgggt
    15841 gataactgcc agctactgat ttccggggcc gacgaacagg aagggcacca gcgtttaagc
    15901 caatggctgc gcgatgaatt cccccactgc gatgcgccgc tggcggaagt taaatctgac
    15961 gaactggaac cactgccggt ttcactgacc aatctgaatc cgcaaattat ccgcgcccgc
    16021 accgtgtgca gcggtagtgc aggcggcatt ctgacgccga tctcttcttt agatctcaat
    16081 gcgctgagta atcttcccgc agccaaagac gttgacgccg agcaatccgc actggaaaac
    16141 ggcctgacgc tggtactgaa aaacattgag tttcgtctgc tggatagcga cggtgctacc
    16201 agcgcgattc tggaagcgca ccgatccctg gctggcgata cttccctgca cgaacattta
    16261 ctggcaggcg tcagcgccgg attaagctgt gccgaagcga ttgtcgccag tgcgaatcac
    16321 ttttgcgaag agtttgcccg ttccagcagc agatacctgc aagaacgtgc cctggacgta
    16381 cgcgacgtct gcttccagtt actccagcaa atctacggtg agcaacgctt cccggcaccg
    16441 ggcaaactga cgcagtccgc catttgtatg gctgatgaac tgacccccag ccagttcctc
    16501 gaactggata aaaatcacct caaaggattg ttactgaaaa gcggcggcac cacctcacat
    16561 acggtgatcc ttgcccgttc gttcaacatt ccgacgctgg ttggtgtgga tattgatgcc
    16621 ctcactccgt ggcagcatca aacgatttat atcgacggca acgccggggc gattgtggtt
    16681 gagccaggag aagccgtagc tcgttattat cagcaagaag cccgcgtaca ggacgccctg
    16741 cgtgagcaac agcgtgtcta gctgacccaa caagcccgta ccgctgacgg tatccgcatt
    16801 gaaattgccg ctaacatcgc tcactccgtg gaagcgcagg ccgcattcgg caatggtgcg
    16861 gaaggcgttg gtttgttccg cactgaaatg ctctatatgg atcgcaccag tgcaccgggc
    16921 gaaagcgagc tgtacaacat tttttgtcag gcgctggagt ctgccaacgg acgcagcatt
    16981 attgtgcgca ctatggacat tggcggcgac aaacccgttg attatctaaa cattcccgca
    17041 gaggcaaacc cgttcctcgg ttatcgcgcc gtgcgtattt atgaagaata cgcgtcgtta
    17101 tttaccacac aactacgctc gatcctgcgc gcctccgctc acggcagcct gaaaatcatg
    17161 atcccgatga tctcctcaat ggaagagatc ttatgggtga aagaaaaact ggcagaagcc
    17221 aaacagcaac tacgtaacga acacattccg tttgatgaga agatccagct cggcattatg
    17281 ctggaagtac cgtcggtgat gttcatcatc gatcaatgct gcgaagagat tgatttcttt
    17341 agtattggta gtaatgacct gacgcaatat ctgctggcag tggatcgcga taacgctaag
    17401 gttactcgtc actacaacag cctgaatccg gcattcttgc gggcgctcga ttacgacgtg
    17461 caggcggtgc atcgccaggg taaatggatt ggtctgtgcg gtgagctggg agcgaaaggt
    17521 tccgtgctgc cgttgctggt cggcttaggg ctggatgaac tcagcatgag cgcaccatca
    17581 attccggcgg cgaaagcgcg gatggcgcaa cttgatagcc gtgagtgccg caagttgctc
    17641 aaccaggcaa tggcctgccg tacttcgctg gaagtggaac acctgctggc gcaattccgc
    17701 atgacccaac aggacgcacc gctggtcacc gccgagtgca tcacgctgga aagtgactgg
    17761 cgcagcaaag aagaagtgct caaaggcatg accgataacc tgctgctggc gggccgctgc
    17821 cgctatccgc gtaaactgga agccgactta tgggcgcgcg aggccgtttt ctctaccggt
    17881 ctgggcttta gttttgccat tccacacagc aaatcagaac acattgagca atccaccatc
    17941 agcgtggcgc gtctgcaagc gccggtgcgc tggggcgatg atgaagcgca attcatcatt
    18001 atgttaaccc tgaacaaaca cgctgcgggc gatcagcaca tgcgcatttt ctcgcgcctc
    18061 gctcgccgca tcatgcacga agaattccgt aacgcgctgg ttaacgccgc ctctgccgac
    18121 gctatcgcca gcctgctgca acatgaactg gaactgtaaa aggaaacatc atggaactgt
    18181 atctggacac cgctaacgtc gcagaagtcg aacgtctggc acgcatattc ccgattgccg
    18241 gggtgacaac taacccgagc attatcgctg ccagcaagga gtccatctgg gaagtgctgc
    18301 cgcgtctgca aaaagcgatt ggtgatgaag gcattctgtt tgctcagacc atgagccgcg
    18361 acgcgcaggg gatggtggaa gaagcgaagc gcctgcgcga cgctattccg ggtattgtgg
    18421 tgaaaatccc ggtgacttcc gaaggtctgg cagcaattaa aatactgaaa aaatagggta
    18481 ttactacact tggcaccgct gtatatagcg ccgcagaagg gttattagcc gcactggcag
    18541 gggcaaaata cgttgctccg tatgttaacc gcgtagatgc ccagggcggg gacggcattc
    18601 gtacggttca ggagctgcaa acgctgttag aaatgcacgc gccagaaagc atggtgctga
    18661 cagccagctt taaaacgccg cgtcaggcgc tggactgttt actggcagga tgtgaatcca
    18721 tcaccctgcc cttagatgta gcgcaacaaa tgctcaacac ccctgcggta gagtcagcta
    18781 tagagaagtt cgaacacgac tggaatgccg catttggcac tactcatctc taaaggagca
    18841 attatggacc gcattattca atcaccgggt aaatacatcc agggcgctga tgtgattaat
    18901 cgtctgggcg aatacctgaa gccgctggca gaacgctggt tagtggtggg tgacaaattt
    18961 gttttaggtt ttgctcaatc cactgtcgag aaaagcttta aagatgctgg actggtagta
    19021 gaaattacgc cgtttggcgg tgaatgttcg caaaatgaga tcgaccgtct gcgtggcatc
    19081 gcggagactg cgcagtgtgg cgcaattctc ggtatcggtg gcggaaaaac cctcgatact
    19141 gccaaagcac tggcacattt catgggtgtt ccggtagcga tcgcaccgac tatcgcctcg
    19201 accgatgcac cgtgcagcgc attgtctgtt atctacaccg atgagggtga gtttgaccgc
    19261 tatctgctgt tgccaaataa cccgaatatg gtcattgtcg acaccaaaat cgtcgcgggc
    19321 gcacctgcac gtctgttagc agcgggtatc ggcgatgcgc tggcaacctg gtttgaagcg
    19381 cgtgcttgct ctcgtagcgg cgcgaccacc atggcgggcg gcaagtgcac ccaggcggcg
    19441 ctggcactgg ctgaactgtg ctacaacacc ctgctggaag aaggcgaaaa agcgatgctt
    19501 gccgccgaac agcatgtagt aaccccggcg ctggagcgcg tgattgaagc gaacacctat
    19561 ttgagcggcg ttggttttga aagcggcggc tggcggcggc tcacgcggtg cataacggcc
    19621 tgaccgctat cccggacgcg catcactatt atcacggtga aaagtggcat ttggtacgct
    19681 gacgcagctg gtactggaaa acgcgccggt ggaggaaatc gaaaccgtag ctgcacttag
    19741 ccatgcggta ggtttgccaa taactctcgc tcaactggat attaaagaag atgtcccggc
    19801 gaaaatgcgc attgttgcaa aagcatcttg tgcagaaggt gaaaccatcc acaacatgcc
    19861 tggcggcgcg acgccagatc aggtttacgc cgcactgctg gtagctgacc agtacggtca
    19921 gcgtttcctg caagagtggg aataacctac tccaaactcc cggcttgtcc gggagtttga
    19981 acgcaaaatt gcctgatgcg ctacgcttat caggcctacg caatctctgc aatatattga
    20041 atttgcgtgc ttttgtaggc cggataaggc gttcactccg catccggcac tctgtgccag
    20101 taatctgaaa ctcccacgtt gttcaggagt tttttatccg cttctggaca ttttctccac
    20161 agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg acgaaccaga
    20221 tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac tccttaaaat
    20281 ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt gccatcgcag
    20341 acggtgccag acagcaaatt ggcagcacgc tattttacga tccggcgtat atgcagctta
    20401 cctatcctgg cggtgatgtt ccgcaagaac gcggtgtatg ttccgatgtg gtgatccgcg
    20461 cattgcgcag ccagaaggtc gatttgcaga aactggttca cgaagatatg gcgaagaatt
    20521 ttgctgagta cccgcaaaaa tggaagttaa agcgcccgga cagcaacatc gatcaccgtc
    20581 gggtgcctaa tctggaaacc tggtttagcc gccacaacaa aacacgcccc atcagtaaaa
    20641 atgccggtga ctatcaagcg ggcgatattg tctcctggcg attagataac gggctggcgc
    20701 atattggggt ggcgtcagat ggcttcgccc gcgacggtac gccgctggtg atacataaca
    20761 ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg gggcattatc
    20821 gttattttgt taaataagag atcgccccac atgaagaaaa aattcagatg ctttttacac
    20881 tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc agcacaatac
    20941 cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag agtacgccac
    21001 aaacgggaac ggccagtgtg cttaagctgg caatactggc aggcaagttt ttcagcacaa
    21061 acagccataa gctccacgct agcgccgtcg ccagaatcgc actgtaggcc agcgcccaga
    21121 acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc acactcatca
    21181 ccagcgccgc atacagcatc tgccaggatg ttaacgataa taaatccacg cgcggatgac
    21241 gggcgtatag gcgtttagca acaatcgcgc tcgcccccca actaacaccg gagaggattg
    21301 ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac accaaaaata
    21361 agccgaaagc ggcaatcaga atcgcgaaat attgcccacg tcgcaggcgt tcaccgagaa
    21421 acaacgcggc gaaaatcacc acccagaacg gcatggtata gctcaggatc gccaccttcc
    21481 ccgcacctcc gctgaccaac gcccactgcg ccaaaccaac catcccgcag gtttgtaaca
    21541 gggcaatggc taaggtgtat ttaaacggcg tcgggcgcat tccgcgacca cgtaataaaa
    21601 ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag tcgaaggcac
    21661 cgatgtaact ggtgacttgc ttcatgaaaa tccagctata actccagata agtgtgagca
    21721 caaccaagcc actgattgcc agtgggttgc tctttcctgc ggcagacatg agagatccag
    21781 tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac tgcctgaaag
    21841 acagtcgctg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc atcactttca
    21901 cccatgccgc tacgaagtct ttaacaaact tctcgtgggc atcgctgctg gcgtaaactt
    21961 ccgctactgc acgcaggaca gagttagaac caaataccag atccgcacgg ctggctgtgt
    22021 atttcacttc gccggtttcg cggtcacgtc cttcgaacag ctctttcgat tcgtcagtcg
    22081 ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc aatacgccaa
    22141 cgcggtcagt gaagacgccg tttttgctgc catcgaagtt ggcacccagt acacgcatcc
    22201 cgcccaccag cgcagtcatt tccggcgcgg tcagcgtcag ttgctgtgct ttgtcgatca
    22261 gcagtgactc ggtggtggaa acgtccagac gagcgcgata gttacggaaa ccgtcagcaa
    22321 ttggctccag cagctcaaac atctcaatgt cagtctgatc ctgacgcgca tcaacgcgac
    22381 ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc tcaacaccaa
    22441 ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct ttctggattt
    22501 tctccagaac aggcagagca cgaacggctg cggcgttcac atcccagtcg cgctgcggca
    22561 ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg aaggtagaag
    22621 cagatgccca ggccaccgat accagctcac taacagacag accagaatcc gcaatcgcga
    22681 atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc agcggatctt
    22741 gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc ggccccatat
    22801 ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc tgcggatcgt
    22861 tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc aggtcggtca
    22921 ccagcattgt cggtttacgt ttcttcgacg gatcgaacgg atccgggata atttccggtg
    22981 cgtctaccgc ttcgaactgg attgctccag ccggactgcg ggtctgtacc cactcatact
    23041 tgaacagatt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc cagactactt
    23101 ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc gcccagccta
    23161 aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacgtttgat gtcggaccgg
    23221 caccgtgggt tttacccagc gtatgaccac ccgcaatcag cgccacggtt tcttcgtcgt
    23281 tcatgcccat gttgccgaag gtcgcgcgga tagctgctgc cgcagaaagc ggttcgccgc
    23341 tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat ctcggttgca cccagcggtg
    23401 ctttcgccag cgcttccgga tgacggtgag tcagccaggc tttttcatca ccccagttaa
    23461 catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag gtacggaagc
    23521 cggagttttc cagcgccacg ttacctgcga ggataaacag gtcggcccag gagattttct
    23581 gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcgagg cttacgttat
    23641 ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg ccgcgtccat
    23701 cgattgaacg gtaagtacct gcgccgtgcc aagccatacg aataaacaga ccggcgtagc
    23761 tgccccagtc ggctggccac cacggctgag attctgtcag cagggctttc agatcttttt
    23821 tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc tcacccagcg
    23881 ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt ggccaccagt
    23941 cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg aacgggcatt
    24001 tgccagtggc tgtggtgtta tggatatcgt ctgacgtgct catcaatgtg ctcccctcta
    24061 cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt aatccacgga
    24121 tgcgatagtt gagatcttac atattgttgg ttaaagagat gtagatcaaa ttgatcgtaa
    24181 ttaagctttt ttgattatgt ccgaattcgg acaaatgctt tataaaaaag ggttttagct
    24241 tcatatcctt caagtaatga gggagaaaac aaggctgaca accttatttt cccgccctca
    24301 tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cactgtgtga ttttcacaaa
    24361 agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc gtaactcatt
    24421 tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct taaaatcttc
    24481 accatatcca tggcaatatt cgcgcccacc agtttgcggg tttcggcatc gtcatccagc
    24541 ccgtcaaaca tttgcgccat ccacgccgga atacgcacgt tggtcatgtc ggcaaatttc
    24601 ttcgcctgtt taaagttaga taccggcaaa atgcccggga tgatttccac atcaatgccc
    24661 gccgatacac agcggtcacg aaaacgcagg tagctttcga catcgaagaa gaactgagta
    24721 atcgcgcggt tggctccggc atccactttg cgtttcagat taagcaaatc cgcctgagcg
    24781 ctttttgctt ccgggtgaac ttccggatac gccgccacgg atatatcgaa atctgccact
    24841 tcttttaaca gcgtcaccag gtcagaagca tacatttctg gcttaccact tcccggcggc
    24901 agatcgccac gcagtgccac gatatgacga ataccgttat tccagtagtc gcgtgcaatg
    24961 gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc ttccagacca
    25021 gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc ggagttcgcg
    25081 ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat ggagttccac
    25141 agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac attaatctgc
    25201 ccctggactt ctgccaggct ctgattcagg gcatcccgct ggctggcgtg aaaaaagctc
    25261 ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc tatccattta
    25321 gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa atgaccaatg
    25381 atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa aagaaaatcc
    25441 gatatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt gcttcttgcc
    25501 ggattcgtcg taaacgcttc atccggccta attctatgat aattcaatat atttcattag
    25561 aaaccataga cccgaaaaca tgagtaccgg gcattattaa atttctgaaa ttacaacaac
    25621 tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg cccagcgcca
    25681 ggcccacgga tcaccagcgg attatcgcgg taccagcggc tttcgatggc aaagacgtta
    25741 tcgcatggca gcagtgatgc cagcggatag tcttcacgca ccgcctccac gcccacacgc
    25801 gctttgccgt tggcatcgaa acgcgccacg tagcgcagca ccagccccat ttcgcgggcc
    25861 gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa gaaatggtcg
    25921 atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg atccggttca
    25981 atgttgtaac ccgcttcacg cgccagaatc accagcttgc gcattacgtc tttgccggag
    26041 aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc caccagctcg
    26101 gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc ggagaagatc
    26161 ccactgatcg acaaaatggt atcgccgctg tcgatcagat cgcgcacggt atggttgatc
    26221 ggcaagcccg caccaacggt ggcgttgtat agccagtgac gcccggtttt ttcgaaggcg
    26281 tcgtggatct ggcgatattt attgctgtcg cttgctcccg ccagtttgtt ggcactgatg
    26341 acgtggaaac cgtggctggc gaaatcgaga tactgatcag caagctgctg gctggcggta
    26401 acgtccaaca ccactaaatc atcatacgga tgggcgcgca tccacaggaa aagcgactct
    26461 tcatcctgct cgaccgcttc atcgttgaag aaggctaacg cgcggctggc gtccagcccg
    26521 tcataactca acagactgcg gcggctgtcc accacgcctg ccagcacaaa ttcaaagccg
    26581 gtacgcgccg aaagcgtgct ctgctcgcgg gcgaacagtt ccagccagcg agagccgata
    26641 ttgcctttgc cgaacaatac caggccgatg cgtttttctg cgcggaagac ggactgatgc
    26701 agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag actgatgccg
    26761 tcatcggact gccacgtaaa ctcgaccggc tggcctttca gttgctgcca gaagcggtgg
    26821 caatgcagcg ggttacgggt gacacccgcc ccgaccatcg ccaccagcgc cagcccctga
    26881 cgcaggcgca gttctccagg taatcccgct tcgtcgagga ttttcagcgc actgtcggcc
    26941 acttctgagg tgtagcaaaa ttgcaacaac tggcgatcgt tatgcacgcc aaccgccagt
    27001 ggacgcacct gggcacgttt caggatctgg tcgatctctt tatgcgccag tttgaaatcc
    27061 tgactggcgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt gacaatacgc
    27121 gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc cggcgtgtag
    27181 ctacagcgca gctgcaggtc gatttcgctg ccagaaactg gctgtaaagt acgggcgtga
    27241 agaacgggag ccgccagacg cgccagttcg ctggcctcat ccagacgcag caacggcagc
    27301 aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac gtcgctccag
    27361 atggttacac gagaaacacc cgccagcgca ccgatttgtg tcgcggaata gtcggaaccg
    27421 ttacgcccca gcaacaccgt ttcaccggcg ttgttacggc tgataaaccc ggtcaccacc
    27481 agacgtttgc tcggatgttg taccagtagc tgttgcagca acgggtaaga aagcccttca
    27541 tcaacctgcg gttgcgcagc gcgttcagcg cgtaaaaact cacgggcatc aagccaggcc
    27601 gctggcagcc cttgttgatt aagtaccgca gacatcaggc gcgccgacca cacttcaccg
    27661 tggcccacca cttccgcata caccgcatcg ttaataccac tgtcgagcaa cgccgccagg
    27721 cgctcaagat cgctgacaaa agcgctaatg aggctatcgg cttcttcagc gggtagcaga
    27781 ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg cgcagagaga
    27841 cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct accggcggcg
    27901 gaaaccacca tcatatcgtc aggctgagag aactccgcca taatgcccgc gacacgcaaa
    27961 taacacttca catcagccag actactgcca ccaaatttat gcagctgacg acctttcgcc
    28021 cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag ccattttcca
    28081 ggtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc agcgtctcgg
    28141 agatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg gttgcggcgt
    28201 gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac gacagcccgc
    28261 ccaggaaacg acacagcgtc tgctcatcgc catccagttc aaaactcaac attgcgccaa
    28321 agcctttttg ctgacgcgcg gcaatttcat gcccctgatt ttccggcagc gacgggtgat
    28381 acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc gcgttgcgct
    28441 gcgccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga tagctgtcaa
    28501 acgcgccgcc cgtcacgcca atattgtttg cccaccaggc aagttcagtg acaacgtccg
    28561 ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga tatttcgtac
    28621 atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc gggcttaaga
    28681 aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag attttcgcaa
    28741 tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt ttgggttttt
    28801 ctgccagcgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac acgcgatagc
    28861 aaccgcgttt cgccagactg tcgaacaggc gatagctacc gccgtagcag tcgtgcggcg
    28921 caaccagcag atcgccaggt ttcaaaaaga cggtcgttac caggtgaatc gcggacatgc
    28981 cggtattagt aagtactgcg ccagcaccgc cttccagttc tgccagcgca cgctgaacca
    29041 catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc gcgcggttca ttaaatccgg
    29101 taaagttata ggtgctggaa agatggatgg gagggacaac gcaaccatac tgttcgtcgt
    29161 catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga tgaagtttcc
    29221 tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg gacgtcaata
    29281 catctggaca tctaaacttc tttgcgtata gattgagcaa atcccaaata gccgttaaaa
    29341 ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg cctgtacggt
    29401 aaactatgcg ggtttacggt cagtacccac ataaattgtg gggtctggtc tcaatttatt
    29461 gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca gcccatacgc
    29521 tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc ctcttaaggt
    29581 gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc gtcacgctac
    29641 caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac ctttgccgga
    29701 tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa aagagatcat
    29761 gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcggaaaag agcggcgcgg
    29821 agtggaatcg cctgatgcac tacgcttatc aggcctacgt catattgcaa tttattgaat
    29881 ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgagca
    29941 cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa acaggctgct
    30001 gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccgcaaa ctggcgacat
    30061 catctttcaa atctctcgct catcgcaaag taaagcgatc caactggcaa cccattccga
    30121 ttacagccac accggtatgc tggtgatgcg taacaaaaag ccctacgttt ttgaagcggt
    30181 cggcccggtg aaatacaccc cgctcaagca gtggattgcc catggtgaaa agggcaacta
    30241 cgttgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac tgacgcaaac
    30301 ggcaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg acgatcgcca
    30361 gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc gcgtgggcga
    30421 acagcagaag ttgaaagagt ttgatctcag caacccgctg gtccaggcga agctcaaaga
    30481 acgctacggt aaaaatattc cgcttgagga aacggtagtt tcaccacaag ccgttttcga
    30541 cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt gatacgacca
    30601 aaattcaggc acaaaaaaag cgccgtgcgg cgcttttttc ggaaatccgg tcttatttgc
    30661 tgcccgggat gttgaaacgc ttgttgaagc ggtcaacacg gccaccggta gcaacatcac
    30721 gctgtttgcc agtgaagaac gggtggcact tgctgcacac gtcgaggttc aggtcatgac
    30781 caacggtgga gcggattttt attacgttac cgcaagagca gctagcagta atttcttcgt
    30841 atttcgggtg aatatctttt ttcatgggaa aacctcggtt taaggccgcg tcgctcttcc
    30901 agccctaacg ccagacacca cgcgatgtta aaagtatagc ttcaatacga tcatttcgta
    30961 cgaaggcgcg aaatcataca gaaattaacc agcgtatgca aactgatccg cactcttcta
    31021 cggcaatgtg tatactaacc caccgaattt caagtcagga tgatgctatg cccgttgccc
    31081 acgttgcctt gcccgttccg cttcctcgta cctttgacta tctgctgcca gaaggcatga
    31141 cggttaaagc tgggtgtcgc atgcgcgtgc cgtttggcaa acagcaggag cgcatcggga
    31201 ttgtggtatc agttagcgat gccagcgaac tgccgctcac tgagctaaaa gcggtagtcg
    31261 aagtgctgga tggtgagccg gtgtttactc actccgtctg gcgattgctg ctatgggcgg
    31321 cagattacta tcatcatccg attggcgatg tgctgtttca tgccttgccg attttactac
    31381 gccaggggcg gcctgcggcg aacgcgccga tgtggtactg gtttgccact gaacaaggtc
    31441 aggcggtgga tctgaacagc ctgaaacgct cacccaagca acaacaggcg ctggcagcgt
    31501 tacggcaagg caaaatctgg cgcgaccagg ttgccacgct cgaatttaat gatgccgcgt
    31561 tgcaggcgct gcgcaaaaaa ggtctgtgtg atttagcaag tgaaacacca gagtttagcg
    31621 actggcgaac gaactatgcc gtttctggtg agcggttgcg attgaatacc gaacaggcca
    31681 ccgccgttgg cgcaattcat agcgcggcag atactttttc tgcctggctg ctggcgggcg
    31741 ttaccggttc cggtaaaacg gaggtttatc tcagtgtact ggaaaacgtg ctcgctcagg
    31801 gcaaacaggc gctggtgatg gtgccggaaa tcggcctgac accgcaaact atcgcccgtt
    31861 ttcgtgaacg gtttaatgcg cccgtggaag ttctgcattc cggtctgaac gacagcgagc
    31921 gtctttcggc gtggctgaaa gcgaaaaatg gcgaggcggc gattgtgatc ggcacccgct
    31981 ccgcgctgtt tacgccgttt aaaaatctcg gcgtgattgt cattgacgaa gagcacgaca
    32041 gttcctacaa gcagcaggaa ggctggcgct atcatgcccg cgacctggcg gtgtatcgtg
    32101 cgcatagcga gcaaatcccg attattcttg gctccgcaac gcccgcgctg gaaacgttgt
    32161 gcaacgtcca gcagaaaaaa taccgcctgc tgcgcctgac gcgccgggcg gggaatgcgc
    32221 gtccggcaat tcaacacgtg ctggatttaa aaggtcagaa ggtgcaggca ggtctggctc
    32281 cggcgttaat cacccgtatg cgccagcatt tacaggccaa caatcaggtt atcctctttc
    32341 ttaaccgccg tggctttgcg cctgcgctac tgtgccacga ctgcggctgg attgccgaat
    32401 gcccacgttg cgatcactac tacacgctgc atcaggcgca acagcatttg cgctgtcacc
    32461 actgtgacag tcagcgtcca gtgccgcgcc agtgcccttc ctgcggttcc acgcacctgg
    32521 tacccgtggg gctgggcacc gaacagcttg aacagacgct tgcgccgctg ttccctgatg
    32581 tgcccatttc tcgtatcgac cgcgatacca ccagccgcaa aggggcgctg gaacagcaac
    32641 tggcggaagt acatcgcggc ggtgcgagga ttttgatcgg cacacaaatg ctggcgaaag
    32701 gtcaccactt tccggatgtg acgctggtgg cgttactgga tgtggacggc gcgctgtttt
    32761 ctgccgattt tcgctcggca gagcgtttcg ctcagcttta cacccaggtc gctggtcgtg
    32821 ccgggcgtgc gggtaaacaa ggcgaagtgg tgctgcaaac gcaccatccg gaacatcctc
    32881 tgttgcaaac gttgctctat aaaggctacg acgcctttgc cgaacaggcg ctggctgagc
    32941 gacggatgat gcaactgccg ccatggacca gccatgtgat tgtgcgtgcg gaagatcata
    33001 acaatcagca tgcgccgctg ttcctgcaac aactgcgtaa tctgatcctc tccagcccgc
    33061 tggcagacga caaactgtgg gttctcggtc cggttccggc actggcacct aaacgtggcg
    33121 gtcgctggcg ctggcagata ttgttgcagc acccttcccg cgtgcgcttg caacacatca
    33181 ttagtggtac gctggcgctc atcaatacaa taccggattc ccgcaaggtg aaatgggtac
    33241 tggatgttga tccgattgag ggttaaaccg cttacgatgc gaggcggatc gaaaaattca
    33301 atattcatca cacttttcat gaaaattctg taaccgtttt cacgcgctat ctgctaaaaa
    33361 tgttgccgat gtgaagtaaa catggatgta gtacgcctga cgtgccaggc gaggagtgag
    33421 tgtgaaagcg aagaagcagg aaactgccgc gaccatgaaa gacgttgccc tcaaggcaaa
    33481 agtctctaca gcgactgtct cccgagcatt aatgaatccc gataaagtct cccaggccac
    33541 ccgtaatcgg gttgaaaaag cggcccggga agtgggttat ttaccgcagc ctatggggcg
    33601 caacgtcaag cgtaatgaat cccgcaccat tctggtgatt gtcccggata tctgcgatcc
    33661 cttctttagc gaaattattc gcggtatcga agttacggcg gcaaatcacg gatatctggt
    33721 gctgattggc gactgtgcac atcaaaatca gcaggaaaaa acctttatcg atttgatcat
    33781 caccaagcaa attgatggca tgttgctgct gggttcaagg ctgccgtttg atgccagcat
    33841 tgaggaacag cgtaatctgc cgccgatggt gatggcgaac gaatttgcac cggaactgga
    33901 gctgcctacc gttcatatcg acaatctgac cgccgcattt gatgcagtaa attatttata
    33961 tgagcaaggg cataaacgga ttggctgtat agccggtccc gaagagatgc cactgtgtca
    34021 ctaccgcctg caaggctatg ttcaggcgct gcgtcgctgc ggaattatgg ttgatccaca
    34081 atacatcgcc cgtggcgact tcaccttcga agccggaagc aaagcaatgc aacagctgct
    34141 tgatcttcca caaccgccta ctgcagtctt ctgccatagc gatgtgatgg cgctcggcgc
    34201 actttctcag gcaaaacgcc aggggctgaa agtcccggaa gacctttcca taatcggttt
    34261 tgataacatc gacctgacgc aattttgtga tccgccgctg acaaccatcg cgcagccgcg
    34321 ttacgaaatc ggtcgggaag ctatgctgtt attgcttgat caaatgcagg ggcaacacgt
    34381 tggcagtggc tctcgtttaa tggactgcga acttatcatc cggggatcaa cacgcgcgtt
    34441 accttaaagt aaacctttaa gactgccgtg actggtcaaa ggcccgccgc ttaagtaaca
    34501 tggcgggctg acgaacgaat aaatacagcg aaacgatagt ggcacaacga gattatgtac
    34561 gccgcagcca accggcacct tcgcggcgaa aaaagagcac ctcacggaaa aagcaacgaa
    34621 atctgcctgc ggtttctccc gctatggtcg ctattgctgc cgccgttctt gtgaccttta
    34681 tcggtggtct gtacttcatt acgcatcaca agaaagaaga gtccgagacg ctgcaaagcc
    34741 agaaagtgac cggaaacgga ctaccaccaa aaccagaaga acgctggcgc tacattaaag
    34801 agctggaaag tcgccagccg ggtgtgcgtg cgcccacaga accttctgcc ggtggtgaag
    34861 tgaaaacgcc ggagcaactg acaccagaac aacgtcagct tcttgaacaa atgcaggctg
    34921 atatgcgcca gcagccaacg cagctggttg aggttccgtg gaatgaacag acgccggaac
    34981 agcgtcagca aacgctacaa cgccagcgtc aggcgcagca attagcggag cagcaacgcc
    35041 tggctcagca gtcgcgtacg actgaacaaa gctggcagca gcagacgcgt acgtcgcaag
    35101 ccgcgccggt acaggcgcaa ccgcgccagt ctaaaccagc ttctactcag cagccgtacc
    35161 aggatctgct gcaaacccct gcgcacacga ccgcgcagtc gaagccgcag caagctgcgc
    35221 cggttgctcg tgccgctgac gcgccaaaac cgacggcgga gaaaaaagac gaacgccgct
    35281 ggatggtgca gtgcggttcg ttcagaggcg cggaacaggc agagacggta cgtgctcagc
    35341 tggcgttcga aggatttgac tcgaaaatca ccaccaacaa tggctggaat cgtgtggtca
    35401 ttggcccggt gaaaggcaaa gagaacgcag acagcaccct caatcggttg aagatggcgg
    35461 gtcatacaaa ctgcattcgg ctcgccgccg ggggttgaaa ccctcaaaat cccccccatc
    35521 tataattgca ttatgccccg tacttttgta cggggtttgt actctgtatt cgtaaccaag
    35581 gggtcagctc gtgacaacta tagtaagcgt acgccgtaac ggccatgtgg tcatcgctgg
    35641 tgatggtcag gccacgttgg gcaataccgt aatgaaaggc aacgtgaaaa aggtccgccg
    35701 tctgtacaac gacaaagtca tcgcgggctt tgcgggcggt actgcggatg cttttacgct
    35761 gttcgaactg tttgaacgta aactggaaat gcatcagggc catctggtta aagccgccgt
    35821 tgagctggca aaagactggc gtaccgatcg catgctgcgc aaacttgaag cactgctggc
    35881 agtcgcggat gaaactgcat cgcttatcat caccggtaac ggtgacgtgg tgcagccaga
    35941 aaacgatctt attgctatcg gctccggcgg cccttacgcc caggctgcgg cgcgcgcgct
    36001 gttagaaaac actgaactta gcgcccgtga aattgctgaa aaggcgttgg atattgcagg
    36061 cgacatttgc atctatacca accatttcca caccatcgaa gaattaagct acaaagcgta
    36121 aggatctccc atgtctgaaa tgaccccacg cgaaatcgtc agcgaactgg ataagcacat
    36181 catcggccag gacaacgcca agcgttctgt ggcgattgct ctgcgtaacc gctggcgtcg
    36241 catgcagctc aacgaagagc tgcgccatga agtgaccccg aaaaatatcc tgatgatcgg
    36301 cccgaccggt gtcggtaaaa ctgaaatcgc ccgtcgtctg gctaagctgg cgaatgcgcc
    36361 gttcatcaaa gttgaagcga ccaaatttac cgaagtgggc tacgtcggta aggaagttga
    36421 ttctattatt cgcgatctga ccgatgccgc cgtgaaaatg gtacgcgtcc aggctatcga
    36481 gaaaaaccgt tatcgcgctg aagaactggc agaagaacgt attctcgacg tgctgatccc
    36541 acctgctaaa aacaactggg gacagactga acagcagcag gaaccttccg ctgctcgtca
    36601 ggcattccgc aaaaaactgc gtgaaggcca gcttgatgac aaagaaatcg agatcgatct
    36661 tgccgcagca ccgatgggcg ttgaaattat ggctcctccg ggcatggaag agatgaccag
    36721 ccagctgcag tccatgttcc agaacctggg cggccagaag caaaaagcgc gtaagctgaa
    36781 aatcaaagac gccatgaagc tgctgattga agaagaagcg gcgaaactgg tgaacccgga
    36841 agagctgaag caagacgcta tcgacgctgt tgagcagcac gggatcgtgt ttatcgacga
    36901 aatcgacaaa atctgtaagc gcggcgagtc ttccggtccg gatgtttctc gtgaaggcgt
    36961 tcagcgtgac ctgctgccgc tggtagaagg ttgcaccgtt tccaccaaac acgggatggt
    37021 caaaactgac cacattctgt ttatcgcttc tggcgcgttc cagattgcga aaccgtctga
    37081 cctgatcccg gaactgcaag gtcggctgcc aatccgcgtt gaactgcagg cactgaccac
    37141 cagcgacttc gagcgtattc tgaccgagcc gaatgcctct atcaccgtgc agtacaaagc
    37201 actgatggcg actgaaggcg taaatatcga gtttaccgac tccggtatta aacgcatcgc
    37261 ggaagcggca tggcaggtga acgaatctac cgaaaacatc ggtgctcgtc gtttacacac
    37321 tgttctggag cgtttaatgg aagagatttc ctacgacgcc agcgatttaa gcggtcaaaa
    37381 tatcactatt gacgcagatt atgtgagcaa acatctggat gcgttggtgg cagatgaaga
    37441 tctgagccgt tttatcctat aatcgcgttc aatcattttc atcattgttt gatggggctg
    37501 aaaggcccca tttttattgg cgcgtattat gactgaacaa caaattagcc gaactcaggc
    37561 gtggctggaa agtttacgac ctaaaaccct ccccctcgcc tttgctgcaa ttatcgtcgg
    37621 gacggcgctg gcatggtggc aaggtcactt cgatccgctg gtcgccctgc tggcactaat
    37681 taccgccggg ctattacaga tcctttctaa cctcgccaat gattacggcg atgcggtaaa
    37741 aggcagcgat aaacctgacc gcattgggcc gctacgcggc atgcaaaaag gggtcattac
    37801 ccagcaaaag atgaaacggg cgctcattat taccgtcgtg ctcatctgtc tctccgggct
    37861 ggcactggtt gcagtggcat gccatacgct ggccgatttt gtcggtttcc tgattcttgg
    37921 cgggttgtcg atcattgccg ctatcaccta caccgtgggc aatcgtcctt atggttatat
    37981 cggtctgggt gatatttccg tactggtttt ctttggctgg ttgagtgtca tggggagctg
    38041 gtatttacag gctcatacat tgattccggc actgatcctt ccggcgaccg catgcggcct
    38101 gctggcaacg gcagtactga atattaataa cctgcgtgat atcaatagcg accgcgaaaa
    38161 tggcaaaaac acgctggtgg tgcgcttagg tgaagtgaac gcgcgtcgtt atcatgcctg
    38221 cctgctgatg ggctcgctgg tgtgtctggc gctgtttaat ctcttttcgc tgcatagcct
    38281 gtggggctgg ctgttcctgc tggcggcacc attactggtg aagcaagccc gttatgtgat
    38341 gcgggaaatg gacccggtgg cgatgcgacc aatgctggaa cgtactgtca agggagcgtt
    38401 actgactaac ctgctgtttg ttttagggat attcctaagc cagtgggcag cataactgac
    38461 aaatatcaat taacaattga tgattttgcc aacagcccac atagcgcgat atactgaaaa
    38521 ttctcgcagc aactgaatgt taagcctatg aaatacgata cttccgagct ttgtgacatc
    38581 tatcaagaag atgttaacgt cgtggaaccg ctgttctcca actttggcgg acgggcgtcg
    38641 tttggcggac aaataatcac ggtaaaatgt ttcgaggaca acgggttgct gtacgatctg
    38701 ctcgaacaga atggccgtgg tcgtgttctt gtcgtcgatg gcggtggttc tgttcgtcgc
    38761 gcactggtcg atgctgaact ggcgcgtctg gcagtacaaa atgaatggga aggtctggtc
    38821 atttacggcg cggtgcgtca ggtagatgac ctggaagagt tggatatcgg catccaggcg
    38881 atggcggcaa ttccggttgg tgccgctggc gaaggcattg gcgaaagcga tgtccgcgtc
    38941 aattttggcg gtgtcacctt cttctccggc gaccatcttt atgccgacaa taccgggatt
    39001 attctttcag aagatccgct ggatattgaa tgataagaaa ggcaccgcaa ggtgcctttt
    39061 ttctgcgtta cctgttggcc tacacagtaa agaaattacg cgaaagatga agcgtaatca
    39121 gacctcttcc atgcgaccca gcagggcctg cagacgttcc tgccagccgt tctgctgttc
    39181 tttcagatgg ttgttctcac gctccagctc ttcgcgctga tgctgggcat tttgaacttc
    39241 ctgcgacagt gagttgtttt tttctttcag ctcttcgatt tccatctgca acagagtgat
    39301 ggtatcaatc gcctgctgta cttttgcttc cagtttctca aacacttcta atgacattgt
    39361 catacctctc ctgaattgca aggcgttgat ggataaaaaa tcctcgtccc gattaccggt
    39421 gacgccttaa taaatacgag cgcactttag ttagctccga ttgtatgaag ccgcgccatc
    39481 gctgtccagc ggcacgcctt gcagattacg gtttgccata cttttcatcc ttctcctagt
    39541 gacataatcc acatcaatcg aaaatgttaa taaatttgtt gcgcgaatga tctaacaaac
    39601 atgcatcatg tacaatcaga tggaataaat ggcgcgataa cgctcatttt atgacgaagc
    39661 acacacattt taagttcgat atttctcgtt tttgctcgtt aacgataagt taacagcatg
    39721 cctacaagca tcgtggaggt ccgtgacttt cacgcataca acaaacatta actcttcagg
    39781 atccgattat gagtcaaaca tcaaccttga aaggccagtg cattgctgaa ttcctcggta
    39841 ccgggttgtt gatttttttc ggtgtgggtt gcgttgcagc actaaaagtc gctggtgcgt
    39901 cttttggtca gtgggaaatc agtgtcattt ggggactggg ggtggcaatg gccatctacc
    39961 tgaccgcagg ggtttccggc gcgcatctta atcccgctgt taccattgca ttgtggctgt
    40021 ttgcctgttt cgacaagcgc aaagttattc cttttatcgt ttcacaagtt gccggcgctt
    40081 tctgcgctgc ggctttagtt tacgggcttt actacaattt atttttcgac ttcgagcaga
    40141 ctcatcacat tgttcgcggc agcgttgaaa gtgttgatct ggctggcact ttctctactt
    40201 accctaatcc tcatatcaat tttgtgcagg ctttcgcagt tgagatggtg attaccgcta
    40261 ttctgatggg gctgatcctg gcgttaacgg acgatggcaa cggtgtacca cgcggccctt
    40321 tggctccctt gctgattggt ctactgattg cggtcattgg cgcatctatg ggcccattga
    40381 caggttttgc catgaaccca gcgcgtgact tcggtccgaa agtctttgcc tagctggcgg
    40441 gctggggcaa tgtcgccttt accggcggca gagacattcc ttacttcctg gtgccgcttt
    40501 tcagccctat cgttggcgcg attgtaggtg catttgccta ccgcaaactg attggtcgcc
    40561 atttgccttg cgatatctgt gttgtggaag aaaaggaaac cacaactcct tcagaacaaa
    40621 aagcttcgct gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa tatatcgttg
    40681 cgctcgacca gggcaccacc agctcccgcg cggtcgtaat ggatcacgat gccaatatca
    40741 ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa accaggttgg gtagaacacg
    40801 acccaatgga aatctgggcc acccaaagct ccacgctggt agaagtgctg gcgaaagccg
    40861 atatcagttc cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa accactattg
    40921 tctgggaaaa agaaaccggc aagcctatct ataacgccat tgtctggcag tgccgtcgta
    40981 ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga agattatatc cgcagcaata
    41041 ccggtctggt gattgacccg tacttttctg gcaccaaagt gaagtggatc ctcgaccatg
    41101 tggaaggctc tcgcgagcgt gcacgtcgtg gtgaattgct gtttggtacg gttgatacgt
    41161 ggcttatctg gaaaatgact cagggccgtg tccatgtgac cgattacacc aacgcctctc
    41221 gtaccatgtt gttcaacatc cataccctgg actgggacga caaaatgctg gaagtgctgg
    41281 atattccgcg cgagatgctg ccagaagtgc gtcgttcttc cgaagtatac ggtcagacta
    41341 acattggcgg caaaggcggc acgcgtattc caatctccgg gatcgccggt gaccagcagg
    41401 ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc gaagaacacc tatggcactg
    41461 gctgctttat gctgatgaac actggcgaga aagcggtgaa atcagaaaac ggcctgctga
    41521 ccaccatcgc ctgcggcccg actggcgaag tgaactatgc gttggaaggt gcggtgttta
    41581 tggcaggcgc atccattcag tggctgcgcg atgagatgaa gttgattaac gacgcctacg
    41641 attccgaata tttcgccacc aaagtgcaaa acaccaatgg tgtgtatgtg gttccggcat
    41701 ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg cggggcgatt ttcggtctga
    41761 ctcgtggggt gaacgctaac cacattatac gcgcgacgct ggagtctatt gcttatcaga
    41821 cgcgtgacgt gctggaagcg atgcaggccg actctggtat ccgtctgcac gccctgcgcg
    41881 tggatggtgg cgcagtagca aacaatttcc tgatgcagtt ccagtccgat gttctcggca
    41941 cccgcgttga gcgcccggaa gtgcgcgaag tcaccgcatt gggtgcggcc tatctcgcag
    42001 gcctggcggt tggcttctgg cagaacctcg acgagctgca agagaaagcg gtgattgagc
    42061 gcgagttccg tccaggcatc gaaaccactg agcgtaatta ccgttacgca ggctggaaaa
    42121 aagcggtcaa acgcgcgatg gcgtgggaag aacacgacga gtaatgtcag gcacctgaat
    42181 cgattgcctg atgctgatcg cctgatgcga cgcttacgcg tcttatcagg cctacgagcc
    42241 aatgccattt tgtgtaggcg ggataaggcg ttcacgccgc atccggcatc acgcagactc
    42301 tgaaaccgtg cgggggcaac cccgcacaca tcaataatcc cacccttccc ctgtgctaca
    42361 cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc catcgaattt
    42421 tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg cggcgataaa
    42481 aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt
    42541 gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct ctacattggt
    42601 gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc gattgaaggc
    42661 acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt aggcgataaa
    42721 ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgattgtcgg gccgggagcc
    42781 aaaggcacca ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt agcagcggcg
    42841 ctcggcaaac cgttgagcga actgacggta acgattctgg ctaaaccacg ccacgatgcc
    42901 gttatcgctg aaatgcagca actcggcgta cgcgtatttg ctattccgga cggcgacgtt
    42961 gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta cggtattggt
    43021 ggcgcgccgg aaggcgtggt ttctgcggcg gtgatccgcg cattagatgg cgacatgaac
    43081 ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc
    43141 gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt attgcgtctg
    43201 ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac caaaggcgat
    43261 ctgctggaag gtattagccg caaaggcaat atcgcgacta ccgaaacgct gctgatccgc
    43321 ggcaagtcac gcaccattcg ccgcattcag tccatccact atctggatcg caaagacccg
    43381 gaaatgcagg tgcacattct ctgagtgatt tgatcgattg agccttccag tccttcggga
    43441 ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca ggagaaaaac
    43501 atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga cgccctgttt
    43561 agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaatttac caagcttggc
    43621 cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc gcccgataat
    43681 cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc acgattggcg
    43741 gcactgaaac cgggcgatga agtgcaggtg gttagcgaag cagctggctt ctttgttctg
    43801 gatgaagtac cggactgcga aacgctatgg atgctggcaa ccggtacagc gattggccct
    43861 tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct ggtcctggtt
    43921 cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga actggaaaaa
    43981 cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc agcggggtcg
    44041 ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc gattggcctg
    44101 ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat
    44161 acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg ccgaccgggc
    44221 catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc acgatgagca
    44281 aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg ttgataaatg
    44341 agtgatgatg accacagaca aggaggtcgc actgctcttt ttgcataatt tccagcagtg
    44401 tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga atatttttcg
    44461 tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata tcttctgttg
    44521 cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga attaacgtca
    44581 ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag gcatcttctt
    44641 cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc ttctaaagca
    44701 actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg cctggcatga
    44761 gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct tataggccac
    44821 gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt tggtcataaa
    44881 agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt
    44941 atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat ctacgacatc
    45001 tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag aataatgcgc
    45061 cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt tgatattgct
    45121 taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg gaaataacaa
    45181 tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat ttattgtcgg
    45241 ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt gtgccaacaa
    45301 acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa cgccccaccg
    45361 cccattgcca gacacccggt aaaatcgctc agttacccgc cagcagcatc cacgccacaa
    45421 tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta acagttactg
    45481 ccgctgttgg ccagagcaag cacacaaggc aaaacgccgc ggtctgaata tcgagtagat
    45541 tcttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct atccaaatcc
    45601 agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt atggtcatgt
    45661 aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc gacagatatc
    45721 gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg tgtacgctgt
    45781 tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa acaccagata
    45841 cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc tccatcagcc
    45901 agtttcgaga agactttaac agccagaatc ccagcctgcc actgaacgaa tttcgtgcca
    45961 tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt aacgagaact
    46021 tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt caaatgacct
    46081 ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag gaatacatgg
    46141 cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag aaaaaactgc
    46201 agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa ggtgcactgc
    46261 gttatgttgt cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa ccgattaagc
    46321 tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag cctttgtgcc
    46381 gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat cttcctttat
    46441 tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta actggaaact
    46501 gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag agctggcagg
    46561 tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata tggcgaagcg
    46621 cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga acctgtccgg
    46681 cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc agtacatcat
    46741 catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga ttgcgaaaaa
    46801 attcgcggtg ctgaaagagc agggtctgac tccggttctg tgcatcggtg aaaccgaagc
    46861 tgaaaacgaa gcgggaaaaa ctgaagaagt ttgcgcacgt cagatcgacg cggtactgaa
    46921 aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg tatgggcaat
    46981 cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat tcatccgtga
    47041 ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt acggcggctc
    47101 tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gacatcgacg gcgcgctggt
    47161 tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg cagaagcggc
    47221 taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact ttccttaact
    47281 cctcgcctta acgcaaaatc tcacactcat gatcctggat ttcctcggct gaagcacggt
    47341 tcagcgtcag tagatttcgt tgtgtcgcca gcaacacaaa cgaattatca ctctgccgta
    47401 ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggcacttct tctgcaagca
    47461 tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc aggtattcat
    47521 gcccgcgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc agatttttat
    47581 ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa tggttttgcg
    47641 tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc ggctggccgt
    47701 attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc ggcgttgaag
    47761 gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc aacagatatt
    47821 gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg acttccgcac
    47881 atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg acaatcttac
    47941 gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca gccacaacgg
    48001 cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt tccctgtacc
    48061 tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt cgcgagaaac
    48121 caccgggttt gataattatc ctagggaaat cagcgtttgc tgatctgatc gaacgtaccg
    48181 ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc atcaatggtg
    48241 aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc gtcgcgcgga
    48301 cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata tttcaggtag
    48361 gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac cgacaccgtt
    48421 ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt ccccagttca
    48481 ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat cccgcgctcg
    48541 acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt tttatacagt
    48601 gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag cgcgtagccc
    48661 caggcagcca ggtagttcca gcgtgcgcca ccagagcttt tcggattagg cgtgatcacc
    48721 gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt gcccttacgc
    48781 accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg tttgatccac
    48841 tctttatcaa tccgcccgcg ttccgcaatc gcgtccacgt cataggccag agccagcgtg
    48901 acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga accaccgtgt
    48961 gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggagct gaatgccttg
    49021 ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag ctgaatatcc
    49081 tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca cttgttcatc
    49141 gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt aaccaaacat
    49201 taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat taaaaaggcg
    49261 gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga tgcggcgcga
    49321 acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt catgtaggcc
    49381 tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc taaaggaatc
    49441 tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt cacctttgaa
    49501 cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct gttcgttctg
    49561 gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt aagcgcccat
    49621 acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga tgtggcccag
    49681 cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca gttcgtcaac
    49741 atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg cgatacccgc
    49801 tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacta caacgaattc
    49861 acagccaccg gcaatggccg cagccagggt cagatcgcca caataacggc ccatcacttc
    49921 caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga tcgcttctac
    49981 aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga tgtcgttgtc
    50041 gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca ttgcacccat
    50101 gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt tcaggttttc
    50161 gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aaacgcgcag aaccgaggaa
    50221 cgtaccgcca cggttgatca tgtcagacac gctgtaacgg tctagctgta ccatacggtc
    50281 ttcatacaga cccagatagc cgtcataaat acccattact tccagacctt ctgtcagcgc
    50341 agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc cgcttgtcaa
    50401 cacacctatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat gaaatctgtt
    50461 gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt ataacaatca
    50521 cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac aaaatgcggg
    50581 tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc tgggtacgac
    50641 ggaacagggg tcctgatgga taatcacatc cgatcccgga aaacgccgta aaatagcctg
    50701 ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt ccatttccaa
    50761 atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt gagcgccgct
    50821 aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcttcat caggcaatgc
    50881 gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat ataaaatata
    50941 gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc aagacaaccc
    51001 cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta gcatatcagc
    51061 ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga caaggataat
    51121 cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg tcatcggtgt
    51181 tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac cggagataaa
    51241 catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg agtgattatc
    51301 gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg cgccgatatc
    51361 caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc accatgcaaa
    51421 aattttaatc agcaatagca acgaagccat cgccgtcgca gcaatcgccg cccgactgac
    51481 cagccgtcca taagattgat tcataaatac tcccgctatc aactgacgct agtataacgg
    51541 aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa aaaccccaca
    51601 tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt actactggga
    51661 acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt gcgtcacgtc
    51721 acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct gctccggcgt
    51781 taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag caatttgctc
    51841 attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt ctgcggtgac
    51901 aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt gccgggcctg
    51961 ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc cgtcgaacat
    52021 atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat cgcctgaacc
    52081 gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga cggcagcggt
    52141 aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat agagagttta
    52201 cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca tggattagcg
    52261 acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa atcctgttag
    52321 ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg gaaggcttca
    52381 acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac agcattgatt
    52441 tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta aaagcacttc
    52501 gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa cttgatcgcg
    52561 ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat gatcgtgagc
    52621 tggtggcacg tattcgcgca atcctgcgcc gttcgcactg gagcgagcaa cagcaaaaca
    52681 acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca ggccgtcagg
    52741 aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc ctgctctatt
    52801 tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag gaagtgttgg
    52861 gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac ctgcgtcgta
    52921 aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt cgcggctatc
    52981 tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca tcttctggct
    53041 gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt cacgccagat
    53101 gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc atgtcgaagc
    53161 ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt tccgggcgat
    53221 tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag gccgcgtgat
    53281 cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg ccgataacgc
    53341 cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt tctccgtgcg
    53401 tgatggcgaa gataattacc aactttatct gattcgtccg gccagtagtt ctcaatccga
    53461 tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca tgttggtcag
    53521 tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta agctgaaaaa
    53581 cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg aagcggggcc
    53641 acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc tggagcgcat
    53701 gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca ccccgctgac
    53761 gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca aggaactcga
    53821 gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt tggtgatgtc
    53881 acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc agttgtggag
    53941 tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt tgacagttaa
    54001 cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa gtgcgctgga
    54061 aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg gctttgcggt
    54121 agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta gcccggaaga
    54181 tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc gtgaatctgg
    54241 cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc gtggctgggt
    54301 gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc cgctgtataa
    54361 gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg
    54421 agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc gggggttatt
    54481 ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc ttttgcatcg
    54541 tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac tgatcgtcat
    54601 caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg ctgacacggg
    54661 aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg ctatacaacc
    54721 accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca aaatggtaat
    54781 tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct ccccagcgga
    54841 aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga ttttcgccaa
    54901 acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag tagagatagt
    54961 gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt ttctccgcct
    55021 gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc tggattttgg
    55081 caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat acatcaaccg
    55141 gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat ttattttatg
    55201 tcagccgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa accggacgaa
    55261 cgttagttca tccg
//
LOCUS       DAJTUN010000010        51983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTUN010000010 DAJTUN010000000
VERSION     DAJTUN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.010"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..286
                     /locus_tag="OO470_000581"
     CDS             <1..286
                     /locus_tag="OO470_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6249935.1"
                     /translation="ANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIPT
                     GNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            262..1872
                     /gene="thiP"
                     /locus_tag="OO470_000582"
     CDS             262..1872
                     /gene="thiP"
                     /locus_tag="OO470_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6249936.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            1856..2554
                     /gene="thiQ"
                     /locus_tag="OO470_000583"
     CDS             1856..2554
                     /gene="thiQ"
                     /locus_tag="OO470_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6249937.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(2668..3432)
                     /locus_tag="OO470_000584"
     CDS             complement(2668..3432)
                     /locus_tag="OO470_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6249938.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(3518..4396)
                     /gene="araC"
                     /locus_tag="OO470_000585"
     CDS             complement(3518..4396)
                     /gene="araC"
                     /locus_tag="OO470_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6249939.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            4735..6435
                     /gene="araB"
                     /locus_tag="OO470_000586"
     CDS             4735..6435
                     /gene="araB"
                     /locus_tag="OO470_000586"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6249940.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            6446..7948
                     /gene="araA"
                     /locus_tag="OO470_000587"
     CDS             6446..7948
                     /gene="araA"
                     /locus_tag="OO470_000587"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6249941.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            8148..8843
                     /gene="araD"
                     /locus_tag="OO470_000588"
     CDS             8148..8843
                     /gene="araD"
                     /locus_tag="OO470_000588"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6249942.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            8918..11269
                     /gene="polB"
                     /locus_tag="OO470_000589"
     CDS             8918..11269
                     /gene="polB"
                     /locus_tag="OO470_000589"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6249943.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            11434..14340
                     /gene="rapA"
                     /locus_tag="OO470_000590"
     CDS             11434..14340
                     /gene="rapA"
                     /locus_tag="OO470_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6249944.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            14352..15011
                     /gene="rluA"
                     /locus_tag="OO470_000591"
     CDS             14352..15011
                     /gene="rluA"
                     /locus_tag="OO470_000591"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6249945.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(15128..15943)
                     /gene="djlA"
                     /locus_tag="OO470_000592"
     CDS             complement(15128..15943)
                     /gene="djlA"
                     /locus_tag="OO470_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6249946.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            16198..18552
                     /gene="lptD"
                     /locus_tag="OO470_000593"
     CDS             16198..18552
                     /gene="lptD"
                     /locus_tag="OO470_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6249947.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            18605..19891
                     /gene="surA"
                     /locus_tag="OO470_000594"
     CDS             18605..19891
                     /gene="surA"
                     /locus_tag="OO470_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6249948.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            19891..20880
                     /gene="pdxA"
                     /locus_tag="OO470_000595"
     CDS             19891..20880
                     /gene="pdxA"
                     /locus_tag="OO470_000595"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6249949.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            20877..21698
                     /gene="rsmA"
                     /locus_tag="OO470_000596"
     CDS             20877..21698
                     /gene="rsmA"
                     /locus_tag="OO470_000596"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6249950.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            21701..22078
                     /gene="apaG"
                     /locus_tag="OO470_000597"
     CDS             21701..22078
                     /gene="apaG"
                     /locus_tag="OO470_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6249951.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            22085..22927
                     /gene="apaH"
                     /locus_tag="OO470_000598"
     CDS             22085..22927
                     /gene="apaH"
                     /locus_tag="OO470_000598"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6249952.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(23005..23484)
                     /gene="folA"
                     /locus_tag="OO470_000599"
     CDS             complement(23005..23484)
                     /gene="folA"
                     /locus_tag="OO470_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6249953.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(23675..25537)
                     /gene="kefC"
                     /locus_tag="OO470_000600"
     CDS             complement(23675..25537)
                     /gene="kefC"
                     /locus_tag="OO470_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6249954.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(25530..26060)
                     /gene="kefF"
                     /locus_tag="OO470_000601"
     CDS             complement(25530..26060)
                     /gene="kefF"
                     /locus_tag="OO470_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6249955.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(26108..27502)
                     /locus_tag="OO470_000602"
     CDS             complement(26108..27502)
                     /locus_tag="OO470_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6249956.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(27561..27848)
                     /gene="fixX"
                     /locus_tag="OO470_000603"
     CDS             complement(27561..27848)
                     /gene="fixX"
                     /locus_tag="OO470_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6249957.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(27845..29131)
                     /locus_tag="OO470_000604"
     CDS             complement(27845..29131)
                     /locus_tag="OO470_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6249958.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(29182..30123)
                     /locus_tag="OO470_000605"
                     /pseudo
     CDS             complement(29182..30123)
                     /locus_tag="OO470_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(30138..30908)
                     /locus_tag="OO470_000606"
     CDS             complement(30138..30908)
                     /locus_tag="OO470_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6249959.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            31380..32894
                     /gene="caiT"
                     /locus_tag="OO470_000607"
     CDS             31380..32894
                     /gene="caiT"
                     /locus_tag="OO470_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6249960.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            32925..34067
                     /gene="caiA"
                     /locus_tag="OO470_000608"
     CDS             32925..34067
                     /gene="caiA"
                     /locus_tag="OO470_000608"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6249961.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            34196..35413
                     /gene="caiB"
                     /locus_tag="OO470_000609"
     CDS             34196..35413
                     /gene="caiB"
                     /locus_tag="OO470_000609"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6249962.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            35487..37040
                     /gene="caiC"
                     /locus_tag="OO470_000610"
     CDS             35487..37040
                     /gene="caiC"
                     /locus_tag="OO470_000610"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6249963.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            37149..37934
                     /gene="caiD"
                     /locus_tag="OO470_000611"
     CDS             37149..37934
                     /gene="caiD"
                     /locus_tag="OO470_000611"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6249964.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            37940..38530
                     /gene="caiE"
                     /locus_tag="OO470_000612"
     CDS             37940..38530
                     /gene="caiE"
                     /locus_tag="OO470_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6249965.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(38616..39011)
                     /gene="caiF"
                     /locus_tag="OO470_000613"
     CDS             complement(38616..39011)
                     /gene="caiF"
                     /locus_tag="OO470_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6249966.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(39273..42494)
                     /gene="carB"
                     /locus_tag="OO470_000614"
     CDS             complement(39273..42494)
                     /gene="carB"
                     /locus_tag="OO470_000614"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6249967.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(42512..43660)
                     /gene="carA"
                     /locus_tag="OO470_000615"
     CDS             complement(42512..43660)
                     /gene="carA"
                     /locus_tag="OO470_000615"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6249968.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(44116..44937)
                     /gene="dapB"
                     /locus_tag="OO470_000616"
     CDS             complement(44116..44937)
                     /gene="dapB"
                     /locus_tag="OO470_000616"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6249969.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(45104..46018)
                     /gene="rihC"
                     /locus_tag="OO470_000617"
     CDS             complement(45104..46018)
                     /gene="rihC"
                     /locus_tag="OO470_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6249970.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(46084..47034)
                     /gene="ispH"
                     /locus_tag="OO470_000618"
                     /gene_synonym="lytB"
     CDS             complement(46084..47034)
                     /gene="ispH"
                     /locus_tag="OO470_000618"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6249971.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(47036..47485)
                     /gene="fkpB"
                     /locus_tag="OO470_000619"
     CDS             complement(47036..47485)
                     /gene="fkpB"
                     /locus_tag="OO470_000619"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6249972.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(47572..48066)
                     /gene="lspA"
                     /locus_tag="OO470_000620"
     CDS             complement(47572..48066)
                     /gene="lspA"
                     /locus_tag="OO470_000620"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6249973.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(48066..50882)
                     /gene="ileS"
                     /locus_tag="OO470_000621"
     CDS             complement(48066..50882)
                     /gene="ileS"
                     /locus_tag="OO470_000621"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6249974.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(50925..51866)
                     /gene="ribF"
                     /locus_tag="OO470_000622"
     CDS             complement(50925..51866)
                     /gene="ribF"
                     /locus_tag="OO470_000622"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6249975.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(51874..>51983)
                     /locus_tag="OO470_000623"
     CDS             complement(51874..>51983)
                     /locus_tag="OO470_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HCR6249976.1"
                     /translation="ICAMAMKSHFFLISVLNRRLTLTAVQGILGRFSLF"
BASE COUNT        12297 a        14275 c        13599 g        11812 t
ORIGIN      
        1 cgcgaacttt agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag
       61 caagcagccg gagctggcgc aaaaattcct ccagtttatg gtttctccgg cttttcagaa
      121 tgcgatccca accggtaact ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt
      181 tgaacaattg accaaaccgg ccaccacgct ggagttcaca ccagctgaag tggcggcaca
      241 acgtcaggca tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta
      301 attccaggtg taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc
      361 ctgtggtgga acgcgccgca gggtaactgg gtggcagtct ggcaggacag ctacctgtgg
      421 catgtggtgc gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc
      481 gcgatattcc tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg
      541 cgtctgtgcg caatgacctt gatcctcccg gtgctagttg ctgttttcgg cattcttagc
      601 gtctatggtc gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc
      661 ttttcgccct acggcctgca aggcattttg ctggcgcacg tattttttaa tctgccgatg
      721 gcgagccgct tattactcca ggcactggaa aacattcccg gcgaacaacg tcagcttgcc
      781 gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg
      841 cgacaaatcc cgccggttgc cgcgcttatc tttatgctct gtttcgccag cttcgccacc
      901 gtgctatcgc tgggcggcgg tccgcaggca accactatcg agctggctat ttatcaggcg
      961 ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt
     1021 tgccttgggc tggtgctgtt gagtcagcga ctaagtaagg ccattgcgcc cggcaccacg
     1081 ctgctgcaag gctggcgcga tccggacgat cgtctgcata gccgcatttg cgacacggtg
     1141 ttaatagtgc tggcgctgct gctgttgctg ccaccgttgc tggcggtgat cgtcgatggg
     1201 gtaaatcgcc agttgccgga agtgctggca caaccggtgc tgtggcaggc gctgtggacc
     1261 tcgttgcgta ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta
     1321 tggagcagtc gcgaactgcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg
     1381 agcggcatgt tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg
     1441 ctcaacaaca ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg
     1501 ttaatggcga tcccttatgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc
     1561 cgctacagta tgttgtgtca gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc
     1621 gagctgcgcg ccctgaaacg tccactggcg caggcgctgg cttttgcctg cgtgctgtcg
     1681 attggtgatt ttggcgtagt ggcgttgttc ggtaacgatg atttccgtac cctgccgttt
     1741 tatctctacc agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt
     1801 ctgctactac tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt
     1861 aaaactgact gatatcacct ggctttatca ccatttgccg atgcgtttta gcttaacggt
     1921 agaacgcggc gagcaggtgg caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct
     1981 gaatttgatt gccggttttc tgacgccagc cagcggttcg ctgactatcg atggtgttga
     2041 tcacaccacg acgccgccgt cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct
     2101 gttcagccat ctgacggtcg cgcagaacat cgggctgggg ctaaatccgg ggttgaaact
     2161 gaacgcggca cagcaggaga aaatgcacgc gattgcccgt cagatgggga ttgataattt
     2221 aatggcgcgg ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg
     2281 ttgtctggtg cgcgaacagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc
     2341 ggcgttacgt caggagatgc tgacgctggt gagcacgagc tgccagcagc aaaaaatgac
     2401 gctattgatg gtgtcgcaca gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt
     2461 ggtcgccgac gggcgcatcg cctggcaggg taagactgaa gaattgttga gcggtaaggc
     2521 gagtgcttcg gcaatattgg ggattacggg ttagtatgca ctgtcagatg cggcgtgaac
     2581 accttatccg acctacggac agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc
     2641 atgaggcaac ccgcacaaga caatccccta aaccccaacc actttacgca aaatatcgat
     2701 atacaccggc atcaacgggt ggcgaattaa caccaccagc gccaccacgc cgatggcaga
     2761 aatcaacggc gtcagccaca acaaacggcc gcgcgacaaa taatgactta aacgatcggt
     2821 tgctttaccg ctgcgccata accgccagca cagccagcca ccaacccaca aaaataccgc
     2881 cgtcgccagc agcaaccatt taaactcgcc gctctgcatt ccggctggaa tatcgatcgc
     2941 cgcgcccgcc agaatcccag gcaggaagta aaacggtggc cacagcaggc agccgataat
     3001 attcggcgta atgaattttg ccaccggcag atccagcatt cccgccacca ttggcaccag
     3061 cggacgcgtc gggccaacaa agcgaccgac cagaatggtg aacatgctgt gttgatgcaa
     3121 cgcatgttca gttttatcga gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa
     3181 cggcttttta aaacgccagc ccagccagaa ggagatccag tcgcccagca gacaaccgac
     3241 aatccctgcc agccaagcgt gccagaaact taactcgccg ctgccaatca gcgctcccag
     3301 ccccgccatc agcaccgtac cgggtagaat caaaccgacc agcgccagcg actccagaaa
     3361 ggccaccaac accaccgcca tcaatgaata cacggtagat tgggtaataa agtgttccag
     3421 caatgcttgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
     3481 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
     3541 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     3601 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     3661 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     3721 agcttaagac gctaatccct aaccgctggc ggaaaagatg tgacagacgc gacggcgaca
     3781 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     3841 tgtactgaca agcctcgcgt acacgattat ccatcggtgg atggagcgac tcgttaatcg
     3901 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     3961 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tacggctggt
     4021 gcgcttcatc cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt
     4081 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     4141 gatgacaacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     4201 agacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     4261 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     4321 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     4381 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     4441 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg
     4501 taaccccgct tattaaaagc attctgtaac aaagcaggac caaagccatg acaaaaacgc
     4561 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     4621 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     4681 tatcgcaact ctctactgtt tctccatacc cgtttttttg gatggagtga aacgatggcg
     4741 attgcaattg gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc
     4801 agcggtgaag agatcgccac cagcgtagag tggtatcccc gttggcaaaa agggcaattt
     4861 tgtgatgccc cgaataacca gttccgtcat catccgcgtg actacattga gtcaatggaa
     4921 gcggcactga aaaccgtgct tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg
     4981 attggcgttg acagtaccgg ctcgacgccc gcaccgattg atgccgacgg taacgtgctg
     5041 gcgctgcgcc cggagtttgc cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac
     5101 actgcggttg aagaagcgga agagattacc cgtttgtgcc acgcgccggg caacgttgac
     5161 tactcccgct atattggcgg tatttattcc agcgaatggt tctgggcaaa aatcctgcat
     5221 gtgactcgcc aagacagcgc cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac
     5281 tgggtgccag ctctgctttc cggtaccacc cgcccgcagg atattcgtcg cggacgttgc
     5341 agcgccgggc ataaatctct gtggcacgaa agctggggcg gcttgccgcc agccagtttc
     5401 tttgatgagc tggacccgat cctcaatcgc catttgcctt ccccgctgtt cactgacacc
     5461 tggactgccg atattccggt gggcacctta tgcccggaat gggcgcagcg tctcggcctg
     5521 cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc atatgggcgc agttggcgca
     5581 ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt ccacctgcga cattctgatt
     5641 gccgacaaac agagcgttgg cgagcgggca gttaaaggta tttgcggtca ggttgatggc
     5701 agcgtggtgc ctggatttat cggtctggaa gcaggccaat cggcgtttgg tgatatctac
     5761 gcctggtttg gtcgcgtact cggctggccg ctggaacagc ttgccgccca gcatccggaa
     5821 ctgaaagcgc aaatcaacgc cagccagaaa caactgcttc cggcgctgac cgaagcatgg
     5881 gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg actggtttaa cggtcgtcgc
     5941 acgccaaacg ctaaccaacg cctgaaaggg gtgattaccg atcttaacct cgctaccgac
     6001 actccgctgc tgttcggcgg tttgattgct gccaccgcct ttggcgcacg cgcaatcatg
     6061 gagtgcttta ccgatcaggg gatcgccgtc aataacgtga tggcgctggg cggcatcgcg
     6121 cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt
     6181 gttgcctctg accagtgctg tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa
     6241 gtgcacgcag acatcccatc agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg
     6301 caaccgcgca gcgaacaggc acaacgcttt gaacagcttt atcgccgcta tcagcaatgg
     6361 gcgatgagcg ccgaacaaca ctatcttcca acttccgccc cggcacaggc tgcccaggcc
     6421 gttgcgactc tataaggaca cgataatggc gatttttgat aattatgaag tgtggtttgt
     6481 cattggcagc cagcatctgt atggcccgga aaccctgcgt caggtcaccc aacatgccga
     6541 gcacgttgtt aatgcgctga atacggaagc gaaactgccc tacaaactgg tgttgaaacc
     6601 gctgggcacc acgccggatg aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg
     6661 ttgcgctggt ctggtggtgt ggctgcacac cttctccccg gccaaaatgt ggatcaacgg
     6721 cctgaccatg ctcaacaaac cgttgctgca attccacacc cagttcaacg cggcgctgcc
     6781 gtgggacagt atcgatatgg actttatgaa cctgaaccag actgcacatg gcggtcgcga
     6841 gttcggcttc attggcgcgc gtatgcgtca gcaacatgcc gtcgttaccg gtcactggca
     6901 ggataaacaa gcccatgagc gtatcggctc ctggatgcgt caggcggtct ctaaacagga
     6961 tacccgtcat ctgaaagtct gccgatttgg cgataacatg cgtgaagtgg cggtcaccga
     7021 tggcgataaa gttgccgcac agatcaagtt cggtttctcc gtcaatacct gggcggttgg
     7081 cgatctggtg caggtggtga actccatcag cgacggcgat gttaacgcgc tggtcgatga
     7141 gtacgaaagc tgctacacca tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa
     7201 cgtgctggaa gcggcgcgta ttgagctggg gatgaagcgt ttcctggaac aaggtggctt
     7261 ccacgcgttc accaccacct ttgaagattt gcacggtctg aaacagcttc caggtctgcc
     7321 cgtacagcgt ctgatgcagc agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc
     7381 cgccctgctt cgcatcatga aggtgatgtc aaccggtctg cagggcggca cctcctttat
     7441 ggaggactac acctatcact tcgagaaagg taatgacctg gtgctcggct cccatatgct
     7501 ggaagtctgc ccgtcgattg ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg
     7561 tattggtggt aaggacgatc ctgcccgact gatcttcaat acccaaaccg gtccagcgat
     7621 tgtcgccagc ctgattgatc tcggcgatcg ttaccgtctg ctggttaact gtatcgacac
     7681 ggtgaaaaca ccgcactccc tgccgaaact gccggtggcg aatgcactgt ggaaagcgca
     7741 accggatctg ccaactgctt ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt
     7801 cttcagccat gcgctgaacc tcaacgatat gcgccagttc gccgagatgc acgacattga
     7861 aatcacggtg attgataacg acacccgcct gccagcgttt aaagacgcac tgcgctggaa
     7921 cgaagtgtat tacggatttc gtcgctaagt agccacatca ggtatgtaac gcctgatgcg
     7981 acgctgacgc gtcttatctg gcctacacgc tgcgattttg taggccggat aagcaaagcg
     8041 catccggcat tcaacgcctg atgcgacgct ggcgcgtctt atcaggccta cgcgctgcga
     8101 ttttgtagac cggataagcg aagcgcatcc ggcacgaagg agtcaacatg ttaaaagatc
     8161 tcaaacgcca ggtattagaa gccaacctgg cgctgccaaa acacaaccta gtcacgctca
     8221 catggggcaa cgtcagcgcc gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg
     8281 gcgtcgatta cagcgtcatg accgctgacg atatggtcgt ggttagcatc gaaaccggtg
     8341 aagtggttga aggtacgaaa aagccctcct ccgacacgcc aactcaccgg ctgctctatc
     8401 aggcattccc ctccattggc ggcattgtgc atacgcactc gcgccacgcc accatctggg
     8461 cgcaggcggg tcagtcgatt ccagcaaccg gcaccaccca cgccgactat ttctacggca
     8521 ccattccctg cacccgcaaa atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa
     8581 ccggtaacgt catcgtagaa acctttgaaa aacagggtat cgatgcagcg caaatgcccg
     8641 gcgttctggt ccattcccac ggcccgtttg catggggcaa aaatgccgaa gatgcggtgc
     8701 ataacgccat cgtgctggaa gaggtcgctt atatggggat attctgccgt cagttagcgc
     8761 cgcagttacc ggatatgcag caaacgctgc tggataaaca ctatctgcgt aagcatggcg
     8821 cgaaggcata ttacgggcag taatgactgt ataaaaccac agccaatcaa acgaaaccag
     8881 gctatactca agcctggttt tttgatggat tttcagcgtg gcgcaggcag gttttatctt
     8941 aacccgacac tggcgggaca ccccgcaagg gacagaagtc tccttctggc tggcgacgga
     9001 caacgggccg ttgcaggtta cgcttgcacc gcaagagtcc gtggcgttta ttcacgccga
     9061 tcaggttccc cgcgctcagc atattttgca gggtgaacaa ggctttcgtc tgacaccgct
     9121 ggcgttaaag gattttcacc gccagccggt gtatggcctt tactgtcgcg cccatcgcca
     9181 attgatgaat tacgaaaagc gtctgcgtga aggtggcgtt accgtctacg aggccgatgt
     9241 gcgcccgcca gaacgctatc tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg
     9301 tgatatgcac aatggcacta tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc
     9361 gccgctcaag tgggtttcta tagatattga aaccacccgc cacggtgagc tgtactgcat
     9421 cggcctggaa ggctgcgggc agcgcatcgt ttatatgctg gggccggaga atggcgacgc
     9481 ctcctcgctt gatttcgaac tggaatacgt cgccagccgc ccgctgctgc tggaaaaact
     9541 caacgcctgg tttgcaaacc acgatcccga tgtgatcatc ggctggaacg tggtgcagtt
     9601 cgatctgcga atgctgcaaa aacatgccga gcgttaccgt cttccgctgc gtcttgggcg
     9661 cgataatagc gagctggagt ggcgcgagca cggctttaaa aacggcgtct tttttgccca
     9721 ggctaaaggt cggctaatta tcgacggtat cgaggcgctg aaatccgcgt tctggaattt
     9781 ctcttcattc tcgctggaaa ctgtcgctca ggagctatta ggcgaaggaa aatctatcga
     9841 taacccgtgg gatcgaatgg acgaaattga ccgccgtttc gccgaagata aacctgcgct
     9901 ggcaacttat aacctgaaag attgcgagct ggtgacgcag atcttccaca aaactgaaat
     9961 catgccattt ttactcgaac gagcaacggt gaacggcctg ccggtggatc ggcacggcgg
    10021 ttcggtggca gcgtttggtc atctctattt tccgcgaatg catcgcgctg gttatgtcgc
    10081 gcctaatctc ggcgaagtgc cgccgcacgc cagccctggc ggctacgtga tggattcacg
    10141 gccagggctt tatgattcag tgctggtgct ggactataaa agcctgtacc cgtcgatcat
    10201 ccgcaccttt ctgattgatc ccgtcgggct ggttgaaggc atggcgcaac ctgatccaga
    10261 gcacagtacc gaaggttttc tcgatgcctg gttctcacgg gagaaacatt gcctgccgga
    10321 gattgtgact aacatctggc acgggcgcga tgaagccaaa cgtcagggta ataagccgct
    10381 ttcgcaggcg ctgaagatca ttatgaatgc cttttatggc gtgctcggca ccaccgcctg
    10441 ccgcttcttc gatccgcgcc tggcatcgtc gatcaccatg cgtggtcatc agatcatgcg
    10501 gcagaccaaa gcattgattg aagcgcaggg ctacgacatt atctacggag ataccgactc
    10561 gacttttgtc tggctaaaag gtgcacattc ggaagaagag gcggcgaaaa tcggtcgtgc
    10621 actggtgcag cacgttaacg cctggtgggc ggaaacgctg caaaaacaac ggctgaccag
    10681 cgcattagaa ctggagtatg aaactcattt ctgccgtttt cttatgccaa ccattcgcgg
    10741 agctgatacc ggcagtaaaa agcgctacgc cgggctgatt caggagggcg acaagcagcg
    10801 gatggtgttt aaagggctgg aaaccgtgcg caccgactgg acgccgctgg cccagcagtt
    10861 tcagcaggag ctgtacctgc gtatcttccg caacgagcca tatcaggagt acgtccgcca
    10921 aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg ctggtttacc gtaaacgcct
    10981 tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct catgtacgcg ccgctcgcct
    11041 tgccgatgaa gaaaatcaaa agcgcggtcg ccccttgcaa tatcagaatc gcggcaccat
    11101 taagtacgta tggaccacca acggcccgga gccgctggac taccaacgtt caccactgga
    11161 ttacgaacac tatctgaccc gccagctaca acccgtggcg gagggaatac tcccttttat
    11221 tgaggataat tttgctacac ttatgaccgg gcaacttggg ctattttgag caaaaaaaag
    11281 agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc
    11341 ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc gggtggttat
    11401 tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg
    11461 atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact
    11521 gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc
    11581 gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg
    11641 caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat
    11701 actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact ggtgttcagc
    11761 aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt
    11821 tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt
    11881 cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg tcgccgccac
    11941 gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg
    12001 atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa
    12061 accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg
    12121 tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt tgacaccgaa
    12181 cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgctt ggaacatctc
    12241 tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa
    12301 gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt gccgggcgtt
    12361 ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt
    12421 ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca gaaaaattat
    12481 cgtccggttg cggacgccgt tgccatgctg ctggcaggta acaaactgag caatgacgaa
    12541 ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt gcaggcagca
    12601 aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat
    12661 cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa aggattcccg
    12721 aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa
    12781 gtctccggca ttatgggcgc acgtaaaagt tcggaagatc gtgctcgcga tatgctctac
    12841 ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg
    12901 cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc
    12961 tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt
    13021 cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg
    13081 tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga
    13141 cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa cccggatcta
    13201 ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat tcagatccat
    13261 gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg
    13321 gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat
    13381 ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaagc
    13441 tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga ccgcctgctg
    13501 gaaatccact ccaacggtgg cgaaaaagcc caggaactgg cagaaagcat tgaagagcag
    13561 gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat cggtatcaat
    13621 caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg
    13681 gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg
    13741 gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa cggtctggat
    13801 ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg
    13861 ccggtaggta cgctgttggt tgaactgatt tatgtggttg aagcccaggc tccgaagcag
    13921 ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc
    13981 aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac
    14041 cgtcacaccg gcagcaaact ggttaacgcc gtgcagcagg atgttcacac tatccttcaa
    14101 ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa
    14161 gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg
    14221 aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc
    14281 ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa
    14341 cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc
    14401 tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg tcagtgccgg
    14461 gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg
    14521 cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca
    14581 aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg
    14641 tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg ccgctgattt
    14701 gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga
    14761 cggaatatga agtggtggag tatgcggcgg ataacacggc aagagtggtg ttaaaaccga
    14821 ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat ccgattctcg
    14881 gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg
    14941 cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag
    15001 cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt cacgccgcat
    15061 ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca ggcctacagt
    15121 ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct
    15181 gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat
    15241 cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc gcatcatccg
    15301 tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct
    15361 gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca
    15421 tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca gcaatgacat
    15481 acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga
    15541 tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca
    15601 tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt tgcgccgcag
    15661 tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata tgaatatcag
    15721 cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa
    15781 aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt
    15841 tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca
    15901 gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac
    15961 acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgcg
    16021 gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc
    16081 taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta
    16141 gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg
    16201 aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag
    16261 ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct
    16321 ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg
    16381 gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt
    16441 ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta
    16501 cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg
    16561 ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag
    16621 atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc
    16681 tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc
    16741 gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc
    16801 cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt gccggtgggt
    16861 gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt
    16921 gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg
    16981 cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag
    17041 gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa
    17101 cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc cggggtcatg
    17161 gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag ctacttcaat
    17221 gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt
    17281 ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca ggttttcagc
    17341 gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta ctaccagaat
    17401 gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga
    17461 gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct
    17521 aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta tcagcaaacc
    17581 aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgta
    17641 atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct
    17701 ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat
    17761 cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc tggcctgttc
    17821 cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt
    17881 gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa
    17941 atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac
    18001 aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga gcgttgggga
    18061 ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc
    18121 attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta cgccagcccg
    18181 gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt
    18241 atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc cattgttggg
    18301 gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac
    18361 agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac
    18421 gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg
    18481 agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa
    18541 aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa
    18601 aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt
    18661 cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga
    18721 aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg caaggcagca
    18781 gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat
    18841 catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc
    18901 gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc
    18961 ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga tgattatctc
    19021 tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccacagg aagtcgaatc
    19081 cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat
    19141 cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg aaagccaggc
    19201 gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca
    19261 ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta ttcaggagtt
    19321 gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat
    19381 tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa
    19441 tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac
    19501 tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga gtggtaaaac
    19561 gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta accagggcgg
    19621 tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg ccctgacccg
    19681 cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga
    19741 actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc gtgcataccg
    19801 catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgtgc
    19861 cagcgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact
    19921 cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg
    19981 ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc
    20041 ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactacg
    20101 ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg
    20161 attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac
    20221 ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaagc
    20281 attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg
    20341 atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc
    20401 gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac
    20461 gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg
    20521 cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc
    20581 gacgagctgc gggcgcaggg gatgaaactc aacgggccgc tgcctgccga taccctgttt
    20641 cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt
    20701 cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt
    20761 attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat
    20821 gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga
    20881 ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg
    20941 atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg
    21001 tcgaaatcgg ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc
    21061 tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag
    21121 gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg
    21181 agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt
    21241 tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa
    21301 aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg
    21361 tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta
    21421 caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca acgatgcctc
    21481 acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc
    21541 gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta accggaatgg
    21601 ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga
    21661 actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt
    21721 gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta
    21781 cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg
    21841 gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt
    21901 ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtacaat
    21961 cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt
    22021 ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa
    22081 gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt
    22141 gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg gcgatctggt
    22201 cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt
    22261 acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg
    22321 caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct
    22381 taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc tggtgatggc
    22441 ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg cgcgcgatgt
    22501 agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt acggcgatat
    22561 gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc
    22621 ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc
    22681 gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga
    22741 agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta cgccggaagg
    22801 tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc tgcgctggga
    22861 agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc
    22921 gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt cgcatccggc
    22981 actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc aatagctgtg
    23041 agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt catccggctc
    23101 gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt
    23161 ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca tgatttctgg
    23221 tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta cgcgatcgtc
    23281 cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga
    23341 ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc
    23401 gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc
    23461 taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc gccactatac
    23521 gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct
    23581 gctggcgagt ccacgctctc cccctggact cgccgcatta caatgaaaca aaaacaaaca
    23641 gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc gtttccggtt
    23701 catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa
    23761 ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg ctggtgcgtt
    23821 tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct tccaccatct
    23881 gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta
    23941 aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca cgctccggct
    24001 tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg cgggcaataa
    24061 tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat
    24121 cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc agtaaatcca
    24181 tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt tcgatatggt
    24241 ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtaa
    24301 tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca tcgatctcat
    24361 cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc accagcagaa
    24421 tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct
    24481 ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca ctgccctgcc
    24541 ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt cgggcaatca
    24601 gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg
    24661 ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca
    24721 acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat tccgagcttg
    24781 ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca
    24841 gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc cgcaagccag
    24901 agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat accaccagta
    24961 ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac
    25021 tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata tcctggaaca
    25081 gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt tcattcatcg
    25141 cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat tccgcgacct
    25201 gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa atcaccatct
    25261 gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt ggatcgagtt
    25321 cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc agaatagatt
    25381 cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg atcaggtagc
    25441 caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc gccgatccga
    25501 ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg ccattccagc
    25561 agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg
    25621 cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc
    25681 ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg
    25741 ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa
    25801 gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg aatgctgtac
    25861 cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc ctgctcggcg
    25921 gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc
    25981 agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg atacggatgc
    26041 gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt tttcccacaa
    26101 agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa
    26161 tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg
    26221 acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac
    26281 ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc ggcagtgccc
    26341 aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg gcagaggcgc
    26401 ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag
    26461 agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata tccgggatta
    26521 gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc
    26581 gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa
    26641 tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa ccaacgattt
    26701 gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa
    26761 caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg
    26821 gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact
    26881 cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg aatcgaccaa
    26941 tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa ccaccttcca
    27001 catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatcgcgg
    27061 caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg atcatcgaag
    27121 tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc accaacagtt
    27181 cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa
    27241 tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc gatgtgccaa
    27301 cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg tccagtttca
    27361 gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa
    27421 acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt
    27481 caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga gtcagtacgg
    27541 cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt gccgcgcggg
    27601 tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc gcactccaga
    27661 catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag acctgcgggg
    27721 cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac aacaatgtgc
    27781 ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac attgacggga
    27841 gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg aagcccactt
    27901 tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg tcaatggtga
    27961 acaggtcacg cgccacgccc accgccagtt ccgggttgcc gctaaacatg cgtgggttat
    28021 caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggcca ctctccagat
    28081 gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca
    28141 cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga atggtaaaac
    28201 cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca ccgaccagct
    28261 ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc accagcttgc
    28321 cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc atttgcggca
    28381 ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc gacagggtat
    28441 tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca
    28501 gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc gacttcggta
    28561 actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc
    28621 ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt ttcgcttcaa
    28681 tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg aggttatcga
    28741 cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc aaccaggcat
    28801 caaatttact gcgcaaaacg gagtaagaac gctgggatgg cgaggtttcg tcaccgttgc
    28861 agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca tgggtgatca
    28921 ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga ctgtgggcat
    28981 agagacgccc gccagtaacg ttcttggcac ctgcggaatt gccacgctcg ataactaaca
    29041 cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc cctgcaccga
    29101 cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg cagagccgta
    29161 atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg gaaggatctt
    29221 cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat ttttatcttt
    29281 attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga tctacccgga
    29341 gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac cgacatagcg
    29401 ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca actccgcacc
    29461 tatcgccttg caaagctgtt ctgccagcgc aatgttctct ttgctgccaa tgccgcgacc
    29521 gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt
    29581 tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg tttcgccagt
    29641 gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata
    29701 cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt gtttcactgt
    29761 tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg
    29821 ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca gcaccagacc
    29881 gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt cgatcatccg
    29941 atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga ttgcctgtgc
    30001 gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct gcgcaccgtt
    30061 catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag aaaacgtgtt
    30121 catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc ggcgatctgt
    30181 tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg cgcggcaacc
    30241 tgttgttcag accaggcgtc cacggcgtta aaaccaatat ccgccgccga ccatacctgg
    30301 actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg ggagttgata
    30361 tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt ttcatcttcc
    30421 agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct gacgccgtta
    30481 actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc ataaaggtcg
    30541 gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc tgcagccagt
    30601 gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac cacaatcagt
    30661 tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag ggctttaccg
    30721 cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg gcaagccgct
    30781 tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa gtctaatgaa
    30841 ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata gcaagtaata
    30901 atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt ggtgttaatt
    30961 acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt gaaaatgtca
    31021 cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg aaaacatggc
    31081 gtagcaaaaa gaaattttta atattgtttt atagatcacc aatattgaaa gtcagatgct
    31141 atttttttac ccacaaaaga atataacaaa ctgattaata accaaaatta acgaaaaacg
    31201 ccctctattc actcgaaaca aaaatgtgac accaatcaca gaatacagct tattgaatac
    31261 ccattatgag ttagccatta acgcgtccac gaggttaata ataattatat taaatgttaa
    31321 caaaaataaa acaaacggga acgcaaaata aatattcgtt ttcacagtgg aattaactca
    31381 tgaagaatga aaagagaaaa acgggaatag aaccgaaggt tttctttccg ccattaataa
    31441 tcgtcggcat actttgttgg cttacagtca gagatctgga tgcggcgaat gtcgttatta
    31501 atgctgtatt cagttacgtc accaatgtat ggggatgggc atttgaatgg tatatggtgg
    31561 tgatgctttt cggttggttc tggctggtgt ttggcccgta tgccaaaaag cgtttaggta
    31621 acgaaccgcc agaatttagc accgccagtt ggatctttat gatgttcgcc tcctgtacgt
    31681 ctgctgccgt actgttctgg ggatcgattg agatctacta ctacatctcc accccgccgt
    31741 ttggcttaga accgaactcg acaggagcga aagagttggg gctggcttac agcttgttcc
    31801 actggggacc tctgccgtgg gccacttaca gcttcctttc agtcgccttc gcttacttct
    31861 tctttgtccg caaaatggaa gtgattcgcc ccagctcgac actggtgccg ctggtaggtg
    31921 aaaaacacgc caaagggttg ttcggtacca tcgtcgacaa cttctatctc gtcgccttga
    31981 tcttcgcgat gggtaccagt ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat
    32041 ggttgtttgg cattccgcat accctgcaac tggacgctat catcattacc tgctggatta
    32101 tcctcaacgc catttgcgtc gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg
    32161 tgcgtagtta cctgagcttc ctgatgctgg gttgggtgtt catcgtcagc ggtgccagct
    32221 tcatcatgaa ctacttcacc gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt
    32281 tgttctatac cgatcccatc gctaaaggcg gcttcccgca gggctggacc gtgttctact
    32341 gggcatggtg ggtgatttac gccatccaga tgagtatctt cctcgcccgc atctcccgtg
    32401 gtcgtactgt gcgtgaactg tgcttcggca tggtgatggg gctgacagca tccacctgga
    32461 tcctgtggac cgtgctcggt agtaacactc tgctgttgat ggataaaaac atcatcaaca
    32521 ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat catcgaaacc tgggccgctc
    32581 tgccactcag caccgccacc atgtggggct tcttcatcct ctgctttatt gccaccgtta
    32641 cgctggttaa cgcctgctct tataccctgg cgatgtctac ttgccgcgaa gtacgcgatg
    32701 gtgaagaacc accgctgctg gtgcgtatcg gttggtcaat tctggttggc attatcggta
    32761 ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac cgccattatc gccggaggat
    32821 gcccgctgtt cttcgtcaac attatggtga cgctctcctt tattaaagac gcgaaacaga
    32881 actggaaaga ttaattaacc cccaaaatat caagaggttg aaagatggat tttaatttaa
    32941 atgatgagca ggaactgttt gtcgccggta tccgcgaact gatggccagc gaaaactggg
    33001 aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagcac
    33061 tggcggatat gggtatcgac agtctgctga tccctgaaga gcacggtggt ctggacgcgg
    33121 ggtttgttac tctcgccgcc gtgtggatgg agctgggacg tctgggggca ccaacctatg
    33181 tactgtacca gctgccgggc gggttcaata ccttcctgcg cgaaggcaca caagagcaga
    33241 tcgacaagat tatggctttc cgcggcaccg gtaagcagat gtggaactca gcgattaccg
    33301 aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac ttatacccgt agaaatggta
    33361 agatttatct taatggtagt aagtgtttta ttaccagtag cgcctacacc ccgtacatcg
    33421 tggtgatggc gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa tggtttgttg
    33481 atatgagcaa accgggcatc aaagtgacca aacttgagaa gctcggtctg cgtatggata
    33541 gctgctgtga aatcaccttt gacgatgtgg aactggacga gaaagacatg ttcggtcggg
    33601 aaggtaacgg ctttaaccgc gtcaaagaag agttcgacca tgaacgtttc ctggtagccc
    33661 tgaccaacta cggtacggcg atgtgcgcct ttgaagatgc ggcgcgctac gccaaccagc
    33721 gcgtgcagtt tggcgaggct attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca
    33781 tggcgatcaa attaaactcc atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca
    33841 acggcaccat cacctctggc gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat
    33901 ttgaagtggt ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg ggcaaccacc
    33961 gcatcagccg cttctggcgt gacctgcgtg tagaccgcgt ttccggagga tctgacgaaa
    34021 tgcagatcct gacgctgggt cgtgcggtgc tgaagcaata ccgctaagtc cgtgcgctgc
    34081 ctgatgcgac gctgacgcgt cttatcaggc ctacgggagc acgaatgtaa gccggataag
    34141 gcgtttacgc cgcatccggc agtcatgcgc tacataaatt tttcaggagc taataatgga
    34201 tcatctaccc atgccgaaat tcggaccgct ggccggattg cgcgttgtct tctccggtat
    34261 cgaaatcgcc ggaccgtttg ccggacaaat gttcgcagaa tggggcgcgg aagttatttg
    34321 gatcgagaac gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc cgcagctctc
    34381 ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa gatgaaggcc gcgaagcgtt
    34441 tctgaaatta atggaaacca ccgatatctt catcgaagcc agtaaaggtc cggcctttgc
    34501 ccgtcgtggc attaccgatg aagtactgtg gcagcacaac ccgaaactgg ttatcgctca
    34561 cctgtccggt tttggtcagt acggcaccga ggagtacacc aatcttccgg cctataacac
    34621 catcgcccag gccttcagtg gttacctgat tcagaacggt gatgttgacc agccaatgcc
    34681 tgccttcccg tataccgccg attacttttc tggcctgacc gccaccacgg cggcgctggc
    34741 agcactgcat aaagtgcgtg aaaccggtaa aggcgaaagt atcgacatcg ccatgtatga
    34801 agtgatgctg cgtatgggcc agtacttcat gatggattac ttcaacggcg gcgaaatgtg
    34861 cccgcgcatg agcaaaggta aagatcccta ctacgccggt tgcggtctgt ataaatgtgc
    34921 cgacggctac atcgtgatgg aactggtggg cattacccaa attgaagagt gctttaaaga
    34981 tattggcctc gcacatctac tcggtacgcc ggaaatcccg gaaggcactc agcttatcca
    35041 ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa ctcgatgcct ggctggcggc
    35101 acataccatc gcagaagtta aagaacgctt tgccgaactg aatatcgcct gtgccaaagt
    35161 actgaccgta ccggaactgg aaagcaatcc acagtatgtt gcccgtgaat caatcactca
    35221 gtggcaaacg atggatggtc gcacctgcaa agggccgaac atcatgccga aattcaaaaa
    35281 taaccccgga caaatctggc gcggaatgcc ctcacatggc atggacacgg ctgccatttt
    35341 gaaaaatatc ggctacagcg aaaacgacat tcaggagttg gtcagcaaag gtctggccaa
    35401 agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga ctagcgcccg cccaataaca
    35461 aacgaacaaa aatggataga ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa
    35521 tgtgggacga tctggcggac gtttacggtc ataaaacggc gctgatttgt gaatccagcg
    35581 gcggagtcgt taaccggtat agttatcttg agttaaatca ggagattaac cgcacggcaa
    35641 acctgtttta tacgctgggg attcgcaaag gcaacaaggt tgcactacat ctcgacaact
    35701 gcccggaatt tatcttttgc tggttcgggc tggcaaaaat tggcgcgatt atggtgccga
    35761 ttaacgcccg cctgttacgc gaggaaagcg agtggatctt gcaaaatagc caggcgtgcc
    35821 tgctggtgac cagtgcgcaa ttttatccta tgtatcaaca gattcagcag gaagatgcca
    35881 ctcaattgcg gcacatttgc ctgacagatg tggcacttcc cgctgatgat ggagtgagtt
    35941 catttactca actgaaaaat caacaacctg ccaccttgtg ctatgcaccg ccgctatcga
    36001 ctgacgatac ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg aaaggtgtgg
    36061 tgattaccca ttacaacctg cgcttcgctg gatattactc cgcctggcag tgtgcactgc
    36121 gtgacgatga cgtctacatg acggtaatgc ctgcgtttca tatcgattgc cagtgtactg
    36181 cggcgatggc ggcgttttct gccggggcca cctttgtgct ggtcgagaaa tacagcgccc
    36241 gcgccttctg gggacaggcg cagaagtacc gcgccaccat taccgaatgt attccgatga
    36301 tgatccgtac gttgatggtg cagccgcctt cagcgaacga tcggcaacac cgcctgcggg
    36361 aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga cgcattttgt gaacgcttcg
    36421 gcgttcgctt gctgacgtct tatgggatga cggaaaccat tgtgggcatt atcggcgatc
    36481 gccctagcga taaacgacgc tggccgtcga ttggtcgggc ggggttttgc tacgaagcgg
    36541 agatccgcga cgatcacaat cgcccgctcc cggcaggtga gatcggtgaa atctgtatta
    36601 aaggcgtacc agggaaaacc atcttcaaag agtattttct caacccgaaa gccactgcga
    36661 aagtgctgga agccgacggc tggctacata ccggcgatac cggataccgc gacgaagaag
    36721 gcttttttta tttcgtcgat cgccgctgca atatgatcaa acgcggtggc gagaatgtct
    36781 cctgcgtgga gctggaaaat atcatcgcca cccatccgaa aattcaggac atcgtggttg
    36841 tgggtattaa agattcgatt cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag
    36901 gtgaaacatt gagcgaagag gaattttttc gcttctgcga acaaaatatg gcgaaattta
    36961 aagtgccctc ttatctggag atcagaaaag atctgccacg taattgctcg gggaaaataa
    37021 ttagaaagaa tctgaaataa atgaaacgtc agggaactac cctgccagct aataatcacg
    37081 ccctaaaaca atatgcattt tttgcaggga tgttgtcgtc cctaaaaaag caaaaatgga
    37141 gaaaaggaat gagtgaatca ttacatctga cccgcaatgg atcaattctg gaaattaccc
    37201 ttgatcgccc aaaagcgaat gctattgatg caaaaaccag ctttgaaatg ggcgaagtat
    37261 ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat tattaccggt gccggagaga
    37321 aattcttttc cgcgggctgg gatttaaaag cggcagcaga aggcgaagca ccggatgctg
    37381 actttggccc gggtggtttt gcgggattaa ccgaaatttt caatctcgac aaaccagtta
    37441 tcgcagctgt gaacggctac gcctttggcg gcggtttcga actggcgctg gcggcagatt
    37501 ttattgtttg tgccgataac gccagcttcg ccctgccgga agccaaactg ggcatcgttc
    37561 ctgacagcgg cggtgtgctg cgtctgccga agatcctgcc gcctaccatc gtcaatgaaa
    37621 tggtgatgac cggcagacga atgggcgcag aagaggcgct gcgttggggg atagtcaacc
    37681 gcgtggttag ccaggcggaa ctgatggata acgcccgcga actggctcag cagctggtta
    37741 acagcgcccc gctggcgatt gcggcgctga aagagatcta ccgcaccacc agcgaaatgc
    37801 cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt gaaacactat ccatcggttc
    37861 tgcattcgga agatgccatt gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt
    37921 ggaaaggacg ttaacaaccg tgagctatta cgcctttgag gggttaattc cggtggttca
    37981 cccgacggcg tttgtccatc ccagtgccgt cttgattggc gatgtgattg tgggagccgg
    38041 tgtctacatc ggcccactcg cctcactgcg tggtgactac gggcggttga tcgtgcaagc
    38101 gggagccaat attcaggatg gctgcattat gcatggctac tgcgacactg acaccatcgt
    38161 tggggaaaac ggccatatcg ggcatggagc gatcctgcat ggttgtgtga ttggtcgcga
    38221 tgcattggtc gggatgaaca gcgtaattat ggatggcgcg gtcattggcg aagagagcat
    38281 tgttgccgca atgagctttg tcaaagcagg ctttagtggc gagaaacgcc agttgttgat
    38341 gggtacgcca gcccgcgctg tacgcagtgt tagtgacgac gagttacact ggaaacggtt
    38401 gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat gcatcgttac atgaaacgca
    38461 gccactgagg caaatggagg aaaatcgccc ccgtttgcag gggacgacgg atgtgacgcc
    38521 taaacggtga tcttgccgga tgcgacctgg ttgctggata agatgctaca acatcgtatc
    38581 tggcaagcag gacgcccggt atcaacgaga taaaattaac gacgcatact ctttgacaac
    38641 atcatctgcc acttctgctc gcggttaagt tccgtgagcg gaacggcagg aatacgcggt
    38701 gtctttctta cgccgactaa tttttcccga ctgttattgc gcaggcgcgc gtaaatttgc
    38761 tcatcgatat cgaccacttt aaccagtcgc tgacactggc atccccgccc ttccagctta
    38821 ttagggatca tcttaacttc gcagcttatt tctgtgactt ccgacagaat ataagtcagg
    38881 gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag agatctctct tgctatcacc
    38941 caccgatttt cagccatcat ccattcggcg attaacaagt agagtggttt ttcaacatat
    39001 ccttcacaca tacgaactcc agtttcacaa attaaaagtc tggctatgac tcgttttgaa
    39061 gcaattaaac aaggccatat taaaattgtg gatatatcga tagtatgcaa cttcaccgtc
    39121 gataagtgtg agctaaatcc tgcttatgtc atcaaaaata tcgattcccc aaaagattta
    39181 cttaatgggc aaaaaaaaac ggtcctcatc agagaaccgt attagttatt cgatcaaatt
    39241 agcggatgaa aaatatccgc cacgacacgc tattatttga tctgtgcgtg catttcctgc
    39301 accgaaatta ctttttcagt cgcatcggca ttcagcgcca tcgcggtagc gaaaccaccg
    39361 ttcagggtgg tgtcataatg cactttatat tgcagcgcac tgcgacggat cacgcgggag
    39421 tcttcaatcg cacgacggcc tgaggtggtg ttgatgatgt aggtatattc gccattcttg
    39481 atacggtcct gaatgtgcgg acggccttca tgcaccttgt ttaccagacg cggattgata
    39541 cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat ccagctcgaa gccctgtttc
    39601 agcagttttg ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac ggaaagcagc
    39661 gcacgaccgt gtttcttcat ggtggagttg ctgcccagct gcgctttggc gaacgcttca
    39721 gcgaaggtgc ggcccacgcc catgacttcc ccggtagagc gcatttctgg ccctaacagc
    39781 gggtcaacgc ctgggaattt attgaacggc agcaccactt ctttcaccga gtagtacggc
    39841 ggaataactt ctttggttac gccctgctca gccagcgatt tgccagccat cacacgcgcc
    39901 gccacttttg ccagcggtac gccggtggct ttggagacga acggaacagt acgcgccgca
    39961 cgcgggttta cttcaatcag gtaaacttcg ttgtttttca ccgcaaactg cacgttcatc
    40021 agaccgcgca cctgcaattc gaaggccagt ttctgcacct gctggcgcat cacatcctga
    40081 atttcctgac ttaaggtgta cgctggcagc gaacatgcgg agtcaccgga gtgcacaccc
    40141 gcctgctcga tgtgctccat gatgccgcca atcagcacca tttcgccgtc gcagatggca
    40201 tccacgtcaa cttctactgc gtcatcaagg aaatgatcca gcaacactgg cgcatcgtta
    40261 gacacgctaa ccgccgtctg gaagtagcga cgcaggtcag cttcgtcata gacgatttcc
    40321 atcgcccgac cgccgagaac gtaagacgga cgcaccacca gcgggtagcc aatctctttt
    40381 gccttctcaa ctgccatttc aatagcagta acggtggcgt tcgccggttg tttcagtttc
    40441 agacggtcaa ccgcatgctg gaagcgttca cggtcttccg cacggtcgat agcatccggg
    40501 ctggtgccga taaccggtac gccagcggct tccagtgcac gcgccagttt cagcggggtc
    40561 tgaccgccgt actgaacgat aacgcctttc ggcttctcaa tacgcacgat ttccagcaca
    40621 tcttccagag ttaccggctc gaagtagagg cgatcggaag tgtcgtagtc ggtagagacg
    40681 gtttccgggt tacagttaac cataatggtt tcgtaaccgt cttcacgcag cgccagcgag
    40741 gcgtgtacgc agcagtagtc gaattcgata ccctgaccga tacggtttgg accgccgcca
    40801 agcaccatga ttttttcacg gtcggtagac ggattcgctt cgcactcttc ttcataagtg
    40861 gagtacatgt aagcggtgtc ggtggcaaac tctgccgcac aggtatccac gcgcttgtag
    40921 accgggtgca ggtcatattg gtcacgcagc ttacggattt ccgcttcgcg tacgcccgcc
    40981 agttttgcca agcgcgcatc ggcaaagcct ttgcgtttca gctggcgcag gaattcagcg
    41041 ttcaggccag tgatgcccac ttccgccact ttctcttcca gacgcaccag ttcttcaatc
    41101 tgtaccagga accagcggtc aatgttagtc aggttgaaga cgccgtccac agacaggccc
    41161 gcgcggaaag catcggcgat gtaccagata cgctctgcgc ctgcgtcttt cagttcgcga
    41221 cggattttgg ttaacgcttc cgggtcatcc aggctcactt tcgggtcgaa gccagttgcg
    41281 cccacttcca ggccgcgcag cgctttttgc agggattcct gctgcgtgcg accaatcgcc
    41341 atcacttcgc caaccgattt catctgagtg gtcagacggt cgttagcacc ggcgaatttt
    41401 tcgaagttga agcgaggaat tttagtaacc acatagtcga tggacggctc gaaggaggcc
    41461 ggagtacgtc cgccagtgat gtcgttcatc agttcgtcta gggtgtaacc caccgccagt
    41521 ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg acgccagcgc cgaagaacgg
    41581 gacacgcgtg ggttcatttc gataacaatc aggcgaccgt ttttcgggtt caccgcaaac
    41641 tggacattgg aaccaccggt ttcaacgccg atttcacgca gcaccgccat cgaggcgtta
    41701 cgcatgattt gatattcttt gtcggtcagc gtttgggctg gcgcgacagt gatggagtca
    41761 ccggtgtgga tgcccatcgc atcaaagttt tcgatagagc agacgatgat gcagttgtcg
    41821 tttttatcac gcaccacttc catctcgtac tctttccagc cgatcagcga ctcatcaatc
    41881 agcaactctt tggtcggaga gagatccaga ccgcgggcgc aaatttcttc aaactcttca
    41941 cggttataag cgataccgcc gccgctaccg cccatggtaa aggatgggcg aataatgcac
    42001 gggaagccca cgtcagcggc aacagccagc gcttcttcca tcgtgtgtgc gataccggaa
    42061 cgcgcggttt ccagaccaat tttcttcatc gctacgtcga aacgacggcg gtcttctgct
    42121 ttatcaatcg catcggcagt ggcaccaatc atggtgacac cgaactcttc caacacgccc
    42181 tgacgttcca gctccagcgc gcagttcagc gccgtctgac cgcccatggt tggcagcacc
    42241 gcgtccgggc gctctttttc aataatcttg cgtaccactt cccagtgaat cggctcgatg
    42301 taggtcgcat cggccatttc cgggtcggtc atgatggtcg ccgggttgga gttcaccaga
    42361 atgacgcggt aaccctcttc gcgcagggct ttacatgctt gcgcgccaga gtagtcaaac
    42421 tcacacgcct gaccgataac aatcgggccc gcacccagaa tcaggatact ttttatatct
    42481 gtacgttttg gcatggctct tttactcctg attacttagc ggttttacgg tactgctcaa
    42541 ttaacttgat aaagtggtcg aacaacggcg cggcgtcgtg tggaccaggg ctggcttcag
    42601 ggtgaccctg gaagctgaac gccggcttat cgatgcgatg aatgccctgt aacgtaccgt
    42661 cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa tgttgcttca tccaccgcaa
    42721 aaccgtggtt ctgggcggtg atcatcacca cgtttttctc cacatcttta accggatggt
    42781 tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc accgctcgcc agcgccagca
    42841 gctgatgacc gagacagatg ccgaataccg gaatatcggt ttcgaggaac ttctggatgg
    42901 cggtaatggc gtaatcgcac ggggccgggt cgccaggacc gttggagagg aagatgccgt
    42961 ctggattcat tttcagcaca tcttccgcag aagtttgcgc cggaacgatg gtcaggcgac
    43021 agcctctatc caccagcatc cgcaggatgt tgcgcttggc accaaaatca taagccacga
    43081 cgtggaacgg cagctcgtct tcttttttcg cttctggcag gccgccggtc aacgtccagc
    43141 tcccttgtgt ccagctatag gcttctgcgg tggtcacttc ttttgccaga tccatgccgt
    43201 tcagacctgg gaacgcgcgg gctttttcta acgccagcgc cgcatccggg ttatcgcctg
    43261 cgataatgca gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc ttacgggtat
    43321 cgatatcggc aatcgccacg atgttatggc gcttcaggta agaagagagg tcttcggtat
    43381 tacggaagtt gctggcaatc agcggcaggt cgcgaatcac cagaccttgt gcatgtacct
    43441 gagaagattc ttcatcggcg tcattagtgc cgacattgcc aatatgggga taagtaagag
    43501 taacgatttg acgagaatag gaaggatcag tgaggatttc ttgataaccg gtcattgaag
    43561 tattgaaaac gacttcccca accgccgaac ctgttgcccc tatggcccga ccgtgaaact
    43621 gggttccgtc ttccagaacc aatagcgctg acttaatcaa aacaccctcc agagaatact
    43681 cactcacttt atttgcatat taattcacag tgatgacgta aatcaatgca aatctgctta
    43741 ccgacgaatt tctggcaaac ggcggcattc tggagatatg ggcgctaaaa gtcaacttaa
    43801 tggcaggatt ttttatatta ttttgtggct catgcatgtt ttacaggcat taagcgacaa
    43861 aaacatcatc taacctgttc tggaaaacgc ttgcgcagca agaagattgc atgaaataaa
    43921 aacgagcagg aaaaagtgga ccaaatggtc aaaatttaca ttaaaacaac aaaaatagaa
    43981 gacgaaacat gtaatttttg gcagttatag aaaatttaaa agcataacac caaaaataaa
    44041 agggctaaaa attaaagccc tttataatta ataaattaaa tgtgttactt ttgcatcata
    44101 ataaacattt tgtggttaca aattattgag atcaagcaca tctcgcatat caaaaagacc
    44161 actttcctta ccactcaacc acaaagccga tcttaccgca ccgttagcaa atgtcatgcg
    44221 gctggacgcc ttatgggtga tctccagacg ctcgccaata tcggcaaaca tcgcggtatg
    44281 ttcgccaacg atgtcacctg cacgcacggt agcaaaacca atggtacctg gcacacgttc
    44341 accggtatgg ccttcacgac tgtagaccgc gcaatctttc agatctttat caagggcgtg
    44401 ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc gcatcaactt tatgtctatg
    44461 atgtgcttca ataatttcga tatcggtgta gtcacccatc actttggctg ctttctccag
    44521 cagcttaagc atgacgttaa cgccaacgct aaaattggcg gcaaagacaa tcgcaatatc
    44581 ggcggcggcg tcacgaattg cctgtttacc ggcttcgtca aaccccgtag tgccgatcac
    44641 catccctttg ccatgctgcc gacaaaaagc gagatggttc agcgtacctt ccggacgggt
    44701 aaaatcgata aacacatcaa aatcatcttt tatcgcatca agactgcttt gcacggtaac
    44761 gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg cccagtaaag aagatccttc
    44821 gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc agtgccgcct gaatcaactg
    44881 gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg atgtttgcat catgcatagc
    44941 tattctcttt tgttaatttg catagaccgt tttcagagta accagcgcat cgctacgccg
    45001 ccagccaaaa acgctgataa ttagaaatta atgataaaca gggagggata tcagtgaaag
    45061 gcatgagtcg atgaatgact gcatgccgat aacatgtgac aggttacggc accagagcca
    45121 gcacctcagc cacccactgt tggaagcctt ttacattcag atccaatgcc acctgtacat
    45181 tggctggttt gcccaggcaa ccgtcgatat caaccaccgt cgtgcctgag gtaaattcgc
    45241 cttgagtttc taccgcgaca aaacacggct tgagggtgaa taagtccggg cgcaccagcc
    45301 aggcgatggc gcagagatcg tgcattcgca gaccgctttg catactgccg ctacggtagt
    45361 ggctaaacag ggcgtggagc atttttccgg tgcgattcaa ctccggtaaa gtagcgagat
    45421 agtcaggggt taatattgcc tgattggtga catccagacc gcacatgaca atttcgatgc
    45481 cgctacggaa gacacaggca gccgcttctg gatcggcagc gatattaaac tcggcatttg
    45541 gtgtacagtt gccgcgacca gcagaaccgc ccatgatcac cagacgacga atatacggtt
    45601 tacattccgg gcattgtaaa agtaacagcg caatattggt taacgggccg atggccacca
    45661 gggtaacggg ctctggtgca cgcatcaggg catcccgaat cgccagaaac gccggtatcc
    45721 cgagtggctt tcggttgtgc tcaacaaagt cgtagccagc cattcccgat tcgccgtgca
    45781 cagatgctgc atcacgcggt gcgcgtacca gcggcacagc ggctccctgg gcgagcggaa
    45841 tctccgcatt ccagaaatgc agcagttgca gggcattgcg ggtagttttc tcaaccgaga
    45901 cattacccgc gacggtggtc atcagttgca ggtcgagttc gggggcaaaa atcgcggcgg
    45961 caatggcgac ggcatcgtca atgccggggt cagtatcgag gaagataggt aaacgcatgt
    46021 tttcttcata aaaaatgccg gtaacaagac cggcattttc gcataactta ggctgctaat
    46081 gacttaatcg acttcacgaa tatcgacacg cagctctttc ggcacttcga aaacaatatt
    46141 ttcttcacgg ccttccagcg gaatggcttc accaccaccc aactgctgca aacgtgccac
    46201 cacattctgc accagaatat ccggagccga tgcgcccgca gtcacgccga cgcatttaac
    46261 ctctttcacc cactcttcct ggatatcttt cgcatcgtca atcaaaaacg cgtgtttgcc
    46321 catacgctgg gccagctccg ccagacggtt ggagttggag gagtttttcg aaccgaccac
    46381 caacacaact tccgcctgtt ctgccagggc gcgtaccgct tcctgacggt tagtcgtggc
    46441 gtagcagatg tcatctttgc gcggaccgac aattttcggg aagcgtttac gcagcgcgtc
    46501 gatcacatca gacgtgtcat ccaccgacag cgtggtttgg gtcataaagg agagcttctc
    46561 ttcgtttttg accgtcagtt tccacacatc gtctggcgat tcgaccagat acattccccc
    46621 ttcggggttg ctgtactgcc ccatcgtccc ttccacttcc gggtgcccgg cgtggccgat
    46681 gagaatagat tcttcgccac ggcgactggc gcgggcaact tccatatgca ctttggtcac
    46741 cagcggacag gtggcatcaa acaccgtcaa atcgcggctt ttcgcttcgt tacgaaccgc
    46801 ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg tccggcactt cgctaatctg
    46861 ctcaataaag atagccccac gctcgcgcaa gctatcgacc acgtagcggt tatgcaccac
    46921 ttcgtgacgg acatatatcg gtgcgccgta aatggccagc gcgttttcaa caatgctgat
    46981 agcgcggtct accccggcac aaaaaccacg cgggttggcc aacaggatct gcatgttacg
    47041 cctccagtgc cggatcgatt tccagcactt caatatcaaa atgaacggtc tgcccggcca
    47101 gcggatggtt gaaatcaacg gtaatggagt cgccgttaat ttcgcggatc acaccaggca
    47161 tctcactgcc atccattgcg gtaaaaagca tgattgcgcc aatttctggc tcgcctgcat
    47221 ccataaattc acggcgggag aagtactgaa tcaggtccgg tgacggcacg ccaaacgccg
    47281 catctggctc cagcgagaag gtggttttgt cgcccacttt cagccccagc aggtgttgct
    47341 ccagcccttc agaaagagaa gcatcgccca ggcggaacag cgccggttta ccgttgttgc
    47401 gagtagactc ggcggtggtg ccatcgtcga gttttagcgt gaagtgcacc aggacggcgc
    47461 tattgctctg tacagattca gacatgcagg tttgctctta tttttgagga tttttgccgg
    47521 atgcggccta cgatttcgta gcaatctgta ggccgcatcc ggcagggttt attattgttt
    47581 tttcgctttg gaaggtaaaa acccttccag cacgatcagt gccgcaccga cacaaatggc
    47641 agtatcggca aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc
    47701 gacaacgaag ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag
    47761 cgcgtaagcg atattgttta gcttctgcgt ggccttcgag cgatacatca tcactgccag
    47821 gagcacgcta ataccaatcg caataccggc aaagaaccaa cgctgccagc cgccgctatc
    47881 ggcaaggaaa ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa
    47941 cagcgggacc gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc
    48001 gataatcagc acgactacca ccagccacag ccagcgtagc cctgttgaac agatcgattg
    48061 actcatcagg caaacttacg tttttcaccg tcaccggcga cgttgctgac acagcggccg
    48121 cagatttctg cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg
    48181 cacttctcac cttcggcttt actcaacgcg actttcagcc ctttgagtac ttcgctctgc
    48241 tgagcatcag caggtgcgtc gttatagtct gcaacggtag cgccggaggt caacaggaca
    48301 aatcgtaatt catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag
    48361 gttactgccg cttccagcga gccacccact ttcttgtcgg cacgcgcttg ctcaatgact
    48421 ttgttcactt cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt cattgcttca
    48481 ctgtctgcca gaccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt
    48541 tcgcccggca gatagcccca cacttcatca gcggtgaagg agaggattgg tgccatccag
    48601 cgcaccagcg cttcagcgat gtgatacagc gcagtctggc agctacgacg cgccacgcta
    48661 tccgctttgg cggtgtactg acggtctttg atgatgtcga ggtagaagga acccatctca
    48721 acggagcaga agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc
    48781 ttgaggatgt cttcctgtgc cgctttcgca caacctacgg cccagcgatc cagtaccacc
    48841 atctcttccg gtttcaccat atcttttgct ggatcaaaac cattcaggtt tgccagcagg
    48901 aagcgcgcgg tgttacggat acgacgatag ctatcggcag cacgtttcag gatctcgtca
    48961 gaaacggcca tttcaccggt gtagtcggtt gatgccaccc acagacgcag aatatccgcg
    49021 cccagtttgt tcatcacatc ctgcggcgaa acggtgttgc cgatggattt agacatcttg
    49081 cgaccctgac catccacggt aaagccgtgg gtcagtacct gacgatacgg cgctttgcct
    49141 ttcatcgcgg tggagatcat tagggaagac atgaaccaac cacggtgctg gtcagaacct
    49201 tccagataca tgtccgctgc gtgaccggca aattccggac gcacgtcaac aacagaagag
    49261 tgggtagatc cggagtcaaa ccatacgtcg agagtatccg gcactttcac atactggtca
    49321 gcttcgtcgc cgaggatctc tttcgcatcg agatcccacc acgcctggat gccgtcaact
    49381 tcaacgcgtt tagccacttc ttccatcagt tcgagggtac gcggatgcag ctcttccgtg
    49441 tctttgtgca cgaacagtga catcggtacg ccccaggtgc gctgacggga gatacaccag
    49501 tcaggacggt tagcaaccat cgactcgata cgcgcctggc cccagtccgg gagccactgc
    49561 acgcctttga tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac
    49621 cactgcggtg tcgcgcggaa gatgatcggc gttttgtgac gccagcagca cggatagctg
    49681 tgctgcattt tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc aacgacgatg
    49741 tcgttcgctt tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg cagataagtg
    49801 ccgtccgggc caaccgggtt agcggtttcc aggccgtatt tctgaccgat cacatagtcg
    49861 tccgggccgt ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga
    49921 tcgccgagga ttgccggaac gtcgaagccc ataaacggat gggtaaagcg cagcagctca
    49981 agctctgcac cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt
    50041 acgctttcaa ccagatcttt cgccagaatc acggcctgac cgtcgatctg caccagcgca
    50101 tagtcgaaat caggtgcaat agagattgcg cggttcgcag gcagagtcca cggcgtagtg
    50161 gtccagatca ccagcgagat tgggccgtta acgttgctta cgccaaattt cgctttcagc
    50221 gcatcctgat cgaccgcctg gaaagcgacg tcaatggacg gagaagtttt gtcgtaatac
    50281 tcaacttccg cttctgccag tgcagaacgg cagtctacgc accagtgcac cggcttcgcg
    50341 cctttgtgca ggtgaccatt gccgatgatt ttgcccagcg cgcggatgat gttggcttca
    50401 gttttgaagt ccatagtcag atacgggtgc gaccagtcgc ccagcacgcc cagacggata
    50461 aagtctttgc gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac
    50521 tcggcggcgg tgaatttctc acccggctta ccgtattctt gctctacttt cagctcgatc
    50581 ggcagaccgt ggcagtccca gccaggcaca tacggcgagt cataaccgga aagccctttg
    50641 gacttcacga taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatgctgcca
    50701 ttcgcataag gagggccatc atgcagaatg aaggtttttt tgcctttttt agccgcacgg
    50761 atgatgccgt acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg
    50821 agatcgccac gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc
    50881 atcagattct cggttccgtg tttcggtttg attacataac aggcttaagc cggttttgtt
    50941 agcccaaaaa attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc
    51001 agcgacgcaa atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga
    51061 ccgtaaaggt ccattgcaac atctaacaaa tgcacttcca gctgctggcg aataccggca
    51121 accgttgggc gtgttccgat gtttgccacg ccgggtaacg gcttttcacc gaggcccagc
    51181 acttctaccg cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc
    51241 gtcgggaaac ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatggca
    51301 aacgggtgcc ccagtaaact ttctgccaga gccagattgt catcagccag cgcctgacgc
    51361 acggcggtgc tgctgatacg cacaccacct tcgcaaaaag tttgcgtact ggtgatatcg
    51421 aagccgtatt ccatgccagc tttctgtaat aacaagaaat cgccttcacg accagcgcca
    51481 aagcggaaat catcacctac ggcaagaaat tttacgcgca aatgcttcac caaaagatcg
    51541 ctgatgaaat tttgcgcggt taacgccgca aaacgcctgt cgaaacgcac gcacagcacg
    51601 taatcaacgc cacactctgc aaggtaacgc agtttttccc gcagccgggt cagtcttgcc
    51661 ggggctttat cggtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc
    51721 ggtaagttgc gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga
    51781 tgcacgccgt cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga
    51841 ttatgtatgc cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat
    51901 accttgtaca gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct
    51961 tttcattgcc atggcgcaaa tca
//
LOCUS       DAJTUN010000011        51827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTUN010000011 DAJTUN010000000
VERSION     DAJTUN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.011"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..693)
                     /gene="ycjM"
                     /locus_tag="OO470_000624"
     CDS             complement(<1..693)
                     /gene="ycjM"
                     /locus_tag="OO470_000624"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6249977.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAV"
     gene            complement(905..1252)
                     /gene="pspE"
                     /locus_tag="OO470_000625"
     CDS             complement(905..1252)
                     /gene="pspE"
                     /locus_tag="OO470_000625"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6249978.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1295..1516)
                     /gene="pspD"
                     /locus_tag="OO470_000626"
     CDS             complement(1295..1516)
                     /gene="pspD"
                     /locus_tag="OO470_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6249979.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1525..1884)
                     /gene="pspC"
                     /locus_tag="OO470_000627"
     CDS             complement(1525..1884)
                     /gene="pspC"
                     /locus_tag="OO470_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6249980.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1884..2108)
                     /gene="pspB"
                     /locus_tag="OO470_000628"
     CDS             complement(1884..2108)
                     /gene="pspB"
                     /locus_tag="OO470_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6249981.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2162..2830)
                     /gene="pspA"
                     /locus_tag="OO470_000629"
     CDS             complement(2162..2830)
                     /gene="pspA"
                     /locus_tag="OO470_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6249982.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2997..3974
                     /gene="pspF"
                     /locus_tag="OO470_000630"
     CDS             2997..3974
                     /gene="pspF"
                     /locus_tag="OO470_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6249983.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4094..5359)
                     /gene="puuE"
                     /locus_tag="OO470_000631"
     CDS             complement(4094..5359)
                     /gene="puuE"
                     /locus_tag="OO470_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6249984.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5397..6677)
                     /gene="puuB"
                     /locus_tag="OO470_000632"
     CDS             complement(5397..6677)
                     /gene="puuB"
                     /locus_tag="OO470_000632"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6249985.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6679..8166)
                     /gene="puuC"
                     /locus_tag="OO470_000633"
     CDS             complement(6679..8166)
                     /gene="puuC"
                     /locus_tag="OO470_000633"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6249986.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8316..8873)
                     /gene="puuR"
                     /locus_tag="OO470_000634"
     CDS             complement(8316..8873)
                     /gene="puuR"
                     /locus_tag="OO470_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6249987.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8900..9664)
                     /gene="puuD"
                     /locus_tag="OO470_000635"
     CDS             complement(8900..9664)
                     /gene="puuD"
                     /locus_tag="OO470_000635"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6249988.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9876..11294
                     /gene="puuA"
                     /locus_tag="OO470_000636"
     CDS             9876..11294
                     /gene="puuA"
                     /locus_tag="OO470_000636"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6249989.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11443..11607
                     /locus_tag="OO470_000637"
     CDS             11443..11607
                     /locus_tag="OO470_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6249990.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11597..12982
                     /gene="puuP"
                     /locus_tag="OO470_000638"
     CDS             11597..12982
                     /gene="puuP"
                     /locus_tag="OO470_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6249991.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13116..13361
                     /locus_tag="OO470_000639"
     CDS             13116..13361
                     /locus_tag="OO470_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6249992.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13674..15317
                     /gene="sapA"
                     /locus_tag="OO470_000640"
     CDS             13674..15317
                     /gene="sapA"
                     /locus_tag="OO470_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6249993.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNAAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15314..16279
                     /gene="sapB"
                     /locus_tag="OO470_000641"
     CDS             15314..16279
                     /gene="sapB"
                     /locus_tag="OO470_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6249994.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16266..17156
                     /gene="sapC"
                     /locus_tag="OO470_000642"
     CDS             16266..17156
                     /gene="sapC"
                     /locus_tag="OO470_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6249995.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17156..18148
                     /gene="sapD"
                     /locus_tag="OO470_000643"
     CDS             17156..18148
                     /gene="sapD"
                     /locus_tag="OO470_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6249996.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18150..18956
                     /gene="sapF"
                     /locus_tag="OO470_000644"
     CDS             18150..18956
                     /gene="sapF"
                     /locus_tag="OO470_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6249997.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19024..19377
                     /locus_tag="OO470_000645"
     CDS             19024..19377
                     /locus_tag="OO470_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6249998.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19745..20533
                     /gene="fabI"
                     /locus_tag="OO470_000646"
     CDS             19745..20533
                     /gene="fabI"
                     /locus_tag="OO470_000646"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6249999.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20677..21804
                     /locus_tag="OO470_000647"
     CDS             20677..21804
                     /locus_tag="OO470_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6250000.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21872..23806
                     /gene="rnb"
                     /locus_tag="OO470_000648"
     CDS             21872..23806
                     /gene="rnb"
                     /locus_tag="OO470_000648"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6250001.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23884..23986)
                     /locus_tag="OO470_000649"
                     /pseudo
     CDS             complement(23884..23986)
                     /locus_tag="OO470_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24040..26014
                     /gene="pdeR"
                     /locus_tag="OO470_000650"
                     /pseudo
     CDS             24040..26014
                     /gene="pdeR"
                     /locus_tag="OO470_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26162..26335
                     /locus_tag="OO470_000651"
     CDS             26162..26335
                     /locus_tag="OO470_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250002.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26425..27174
                     /locus_tag="OO470_000652"
     CDS             26425..27174
                     /locus_tag="OO470_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6250003.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27443..27661
                     /gene="osmB"
                     /locus_tag="OO470_000653"
     CDS             27443..27661
                     /gene="osmB"
                     /locus_tag="OO470_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6250004.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(27787..28113)
                     /gene="yciH"
                     /locus_tag="OO470_000654"
     CDS             complement(27787..28113)
                     /gene="yciH"
                     /locus_tag="OO470_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6250005.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28113..28850)
                     /gene="pyrF"
                     /locus_tag="OO470_000655"
     CDS             complement(28113..28850)
                     /gene="pyrF"
                     /locus_tag="OO470_000655"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6250006.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29043..30212)
                     /gene="lapB"
                     /locus_tag="OO470_000656"
     CDS             complement(29043..30212)
                     /gene="lapB"
                     /locus_tag="OO470_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6250007.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30219..30527)
                     /gene="lapA"
                     /locus_tag="OO470_000657"
     CDS             complement(30219..30527)
                     /gene="lapA"
                     /locus_tag="OO470_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6250008.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(30676..31440)
                     /gene="pgpB"
                     /locus_tag="OO470_000658"
     CDS             complement(30676..31440)
                     /gene="pgpB"
                     /locus_tag="OO470_000658"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6250009.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31610..32200
                     /gene="ribA"
                     /locus_tag="OO470_000659"
     CDS             31610..32200
                     /gene="ribA"
                     /locus_tag="OO470_000659"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6250010.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32264..34939)
                     /gene="acnA"
                     /locus_tag="OO470_000660"
     CDS             complement(32264..34939)
                     /gene="acnA"
                     /locus_tag="OO470_000660"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6250011.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35103..35198)
                     /locus_tag="OO470_000661"
     CDS             complement(35103..35198)
                     /locus_tag="OO470_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250012.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35312..35479)
                     /locus_tag="OO470_000662"
     CDS             complement(35312..35479)
                     /locus_tag="OO470_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250013.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35482..35646)
                     /locus_tag="OO470_000663"
     CDS             complement(35482..35646)
                     /locus_tag="OO470_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6250014.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(35941..36915)
                     /gene="cysB"
                     /locus_tag="OO470_000664"
     CDS             complement(35941..36915)
                     /gene="cysB"
                     /locus_tag="OO470_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6250015.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37125..39722)
                     /gene="topA"
                     /locus_tag="OO470_000665"
     CDS             complement(37125..39722)
                     /gene="topA"
                     /locus_tag="OO470_000665"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6250016.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40102..40353
                     /locus_tag="OO470_000666"
     CDS             40102..40353
                     /locus_tag="OO470_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6250017.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40389..41438)
                     /gene="sohB"
                     /locus_tag="OO470_000667"
     CDS             complement(40389..41438)
                     /gene="sohB"
                     /locus_tag="OO470_000667"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6250018.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            41658..42416
                     /locus_tag="OO470_000668"
     CDS             41658..42416
                     /locus_tag="OO470_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6250019.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42413..43003
                     /gene="cobO"
                     /locus_tag="OO470_000669"
     CDS             42413..43003
                     /gene="cobO"
                     /locus_tag="OO470_000669"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6250020.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43043..43915)
                     /gene="rluB"
                     /locus_tag="OO470_000670"
     CDS             complement(43043..43915)
                     /gene="rluB"
                     /locus_tag="OO470_000670"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6250021.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44016..44636)
                     /locus_tag="OO470_000671"
     CDS             complement(44016..44636)
                     /locus_tag="OO470_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6250022.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(44633..45514)
                     /gene="yciV"
                     /locus_tag="OO470_000672"
     CDS             complement(44633..45514)
                     /gene="yciV"
                     /locus_tag="OO470_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6250023.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            45652..45696
                     /gene="trpL"
                     /locus_tag="OO470_000673"
     CDS             45652..45696
                     /gene="trpL"
                     /locus_tag="OO470_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6250024.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    45673..45766
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            45788..47350
                     /gene="trpE"
                     /locus_tag="OO470_000674"
     CDS             45788..47350
                     /gene="trpE"
                     /locus_tag="OO470_000674"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6250025.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47350..48945
                     /gene="trpD"
                     /locus_tag="OO470_000675"
     CDS             47350..48945
                     /gene="trpD"
                     /locus_tag="OO470_000675"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6250026.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            48946..50307
                     /gene="trpCF"
                     /locus_tag="OO470_000676"
     CDS             48946..50307
                     /gene="trpCF"
                     /locus_tag="OO470_000676"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6250027.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50319..51512
                     /gene="trpB"
                     /locus_tag="OO470_000677"
     CDS             50319..51512
                     /gene="trpB"
                     /locus_tag="OO470_000677"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6250028.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51512..>51827
                     /gene="trpA"
                     /locus_tag="OO470_000678"
     CDS             51512..>51827
                     /gene="trpA"
                     /locus_tag="OO470_000678"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6250029.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANL"
BASE COUNT        12302 a        13561 c        13072 g        12892 t
ORIGIN      
        1 aacggcatcc aggcggacat actcagcacc tttctcaagg taacagagta aaacatccac
       61 catcgccagc aacacttcag ggctacggta attcaggtca atttgatcgt cactaaaggt
      121 ggtccataaa tggcgcgtag aattatcatc catttgaaac ggcgttaata gcggtaacgc
      181 acgcggaagg gtgacggcac tgagatcggt tttcggatca accgcaataa agaaatcttc
      241 aaaacccgga agctgttgta aatagttttt aaaccattca ctttttgcag acatatggtt
      301 gcagacaaaa tcaaacatta aatgactgca ttcaccgagt tgctgaatat cctgccactc
      361 ccccgcttca ctggcgacct gatgataatc aattaccgaa aatccatcat cagatgacca
      421 tgggtaaaaa ggtaataaat gaacatgtga aaaaatgctt tgcagccatt gatggtagaa
      481 ctgattaaat atgggtagtg gttttaaatc attactgtga aattgatcgg cataggtaat
      541 taacacgaca tcactttcat cccagtgttt tttacgtcgc tgtgtaatta atcgtttcgc
      601 actctcaaga cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc caccgtagat
      661 ttcgtccatg taatccgtaa ttttctgttt catgttgcct ttccgtggta gcggtcccat
      721 gaggcgagag actactccgc agaaaataaa ctgtcaattg agcacaaggg ttttagtcac
      781 gcaggaaatc attttttacc agggaaaaag cgtatttgtg agctgacgca aataatctat
      841 agggatgtaa ataaggtgtg acagaaaaaa aacggcgcat aagcgccgct catggtgaat
      901 tcttttaacc tttgaccttc ggcattgcga tgtctttcag gccaccggcg ttctcaacgt
      961 gggtatatcc catctcgcta aggagctctt ttgcctgccc ggactggcgt ccggcattgc
     1021 aatacacttt cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc tctttcactt
     1081 ccttcagggg aatattgatg gccccctgaa cgtgctcttg ctgatactgc tctggaacac
     1141 gaacatcgat ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt gccagagcaa
     1201 gtaagccttt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata
     1261 cgcctgagat ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt
     1321 tattagcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc
     1381 ggcgagcaac tgattttatc gcccagcccg ccacacccgc cgggccatag cgcagtgcgg
     1441 taagaagtac cagcttgcct gctaatttga aaccaagctt caccttttgc ccggcctgtt
     1501 gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt
     1561 atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg
     1621 gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc
     1681 cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag
     1741 accgaagaaa atcgacagca ccaccaggat acgcaccagt tttaccggta catcaaaata
     1801 gttggcaatc ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataactt
     1861 tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga
     1921 atagattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcaac cagttgcgct
     1981 aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc
     2041 cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc
     2101 gcgctcataa gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt
     2161 attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat
     2221 catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc
     2281 tttctgcttc cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg
     2341 cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct
     2401 gatgacgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct
     2461 cgccaatctc tttcttcatg cgtgccagcg tatcgtccac cagcgtcact tcatgttcca
     2521 gggacttaat cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct
     2581 ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg
     2641 cttgttcaat acggcgagtc agctgtttct tttctgccaa cgcacgcgcc gaagtagaac
     2701 gtacttcaac cagtgtatct tccatctcct ggatcatcag acgcaccagt ttctgtggat
     2761 cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa
     2821 aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta
     2881 atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca
     2941 acagcaacat gccaggatga attagcgaat tacactaaca agtggcgaat ttcatcatgg
     3001 ccgaatacaa agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg
     3061 tttcgcatct cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta
     3121 aagagttgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt
     3181 cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag
     3241 cgggggcgtt taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgacggcg
     3301 gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggaa aaattattgc
     3361 gtgtgattga gtacggtgaa ctggagcgcg ttggcggtag ccagccgttg caggtgaatg
     3421 tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggtacttttc
     3481 gcgctgacct gctcgaccgg ctggctttcg atgtagtgca actgccgccc ctgcgcgagc
     3541 gcgaaagcga cattatgctg atggcagaac actttgccat tcagatgtgt cgggaaatca
     3601 agctgcctct gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt
     3661 ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca
     3721 ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt ccacctgaag
     3781 aagccatcgc cgtttcagaa aatacctcgc ttccgacgct gccgctggat ttacgcgagt
     3841 ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacagggg aaatataacc
     3901 agaagcgcgc ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa
     3961 agcaccagat ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat
     4021 tcggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta
     4081 ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt
     4141 gcgcatccgg gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg
     4201 tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg
     4261 acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta
     4321 ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc
     4381 ccagttgatt cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg
     4441 cggcagccac cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg
     4501 cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt
     4561 tcgccatcgt cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg
     4621 tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca
     4681 ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgcac tcgccctgca
     4741 ccggttcgaa aataatggcc gcgacctgct tcgcttcgat gtctgattta aacaagcgtt
     4801 cgatggcgtc gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt
     4861 gatacaccga accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca
     4921 gcgccatggt catatatgta cgaccgtgga agccaccgct aaacgcaatc acgccaggtc
     4981 gtccggtatg ggcgcgagca attttcaccg cgttttccac cgcttccgcg ccggtagtga
     5041 agaatgcggt tttagcctgc ccgctcactg gggcaagggt gttaattttc tccgccagcg
     5101 tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct
     5161 gctccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg
     5221 cggcgaaatc gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact
     5281 gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgacgctgat
     5341 ggaattcatt gttgctcatg tttcacacct tttgactctt cactgttaat cggaaattaa
     5401 acgcccagac gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa tggcacgcgc
     5461 agcgtgcgcc cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc gaaacgctcg
     5521 gcgtcaccgc gcagcagttc agcaatcaaa cgcccggcaa gatgggtaca ggtgacgccg
     5581 tggccgctgt aaccctgcat gtaatagatg ttggtatcaa ggcggccgaa ctgcggcatg
     5641 tgcgacaagg tcagcaggaa gttgcccgtc cagcggtaat caattttcac gcccttcaac
     5701 tgagggaagg tttttagtag tttcggtacc acaagacgct caacgtcatc cgggttgcgt
     5761 gcaccataca ctacaccccc gccgtacagc aggcggttgt cggcggtaag acggtagtaa
     5821 tcaagcagat agttacagtc ttccacacag tagtttttcg ggatcagcga acgggctaaa
     5881 tcttccgaca gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct gcgtttcgct
     5941 agttccggct ctactttatc gcccagatac gcattcccgg cgacaatcac atacttcgcc
     6001 gtcacctgac cattggcagt ccgaaccacg gctggcgtgg tgtgctggat ctgcgtcacg
     6061 gcggaaagct catacacgcg cccgccgttc aggcggatgg catcggcttc accaatggcg
     6121 aggtttagcg ggtgaatatg cccaccgctg tgatccagca gcgcaccagt gtagcgatcg
     6181 ctggcgacct cgcggcgaat agcgttgacg tccagcagct ccagctgttt attgccgtag
     6241 cgttcccagt tctctttctg ctcttcgagt gtggcgagtt gtttatcatt catcgccaca
     6301 aacagaccgc cggggcggta gtcgcagtca atctgataac gtttgatacg ttcgcggatg
     6361 atctcgccac cctcgaacat catgctgccg agcatacggg cggtgtccat gccgtagctt
     6421 ttttcgatca cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc attgcgcccg
     6481 cttgcgccaa agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc cgcttccgcc
     6541 agatgcagcg cggaggagag cccggtatag ccgccgccaa ccacgcaaac gtcgcaggtg
     6601 attgactcat tcagtgtgtc gaatggtgca tatttattcg cactggcggc gtagtaactg
     6661 ctggtatgtt cggtcatttc aggcctccag gcttatccag atggttttca gttcagtgaa
     6721 tttttcaagg gcatgcaggg atttgtcgcg accgttgccg ctctgcttat agccgccaaa
     6781 cggcacggtc atatcgccgt cgttgtagtt attgacgaag acggaaccgg ctttcaggcg
     6841 tcggctcatg cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc caaggccgta
     6901 ctggctgtcg ttggcaagct gcagcgcctg atcttctgat gtgaaacggg tgaccaccag
     6961 caccggaccg aaaatctctt cgcggcttaa ggacgcattc gggtccacat ccacgaagat
     7021 ggtcgggccg atggccgctg ccagcccggc gttgcgacca tccagcagca gttgcccctt
     7081 gccttcgcct tcacgaataa aggtgtgtac cgagtcagcg tgagcgctgt cgattaaggt
     7141 gcccatggtg gttgcgggat caagtggatg gcccggctgc cagttttgcg cctgctgttt
     7201 taacagggct aagaattcat cggcgatgct ctcttccagt aacaaacgcg ttccggcgat
     7261 gcacacctgg ccctggttgt agaagatgcc tgcggcggtg gcgcttgccg ctttttgcag
     7321 atccggacaa tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca gccagacacg
     7381 tttcatgttg ctatcacccg cgtctttcag cagttgttta cccgtacgcg tggagccggt
     7441 aaaggcgatg gcatcgatat cgttatgacg cgaaagcgcc tgccccgctt catgaccaaa
     7501 acccgtcacc acgttcaaca caccatccgg caagcctgct tctttcgcca gccccgcgag
     7561 acgaatcgca ctgagcggtg atttttcaga cggttttaga atcacgctgt ttcccgccgc
     7621 cagcgccggg ccgagtttcc agcaagtcag caacagcggg aagttccacg gcacgatggc
     7681 ggcaatcacg ccgaccggtt cacgcacgat catcgccagc tcatggctac tggtggtcgc
     7741 cacttcgcca tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc
     7801 gggaatatca tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag tttccagcag
     7861 tgccagctct tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg
     7921 tttagccgga gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac
     7981 cgcacggtcg atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac
     8041 cggatcaacg gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa
     8101 taagcggttt tcaatggcga gacttaacgc tttatcctgc cagtaagcca gatgatgaaa
     8161 attcattatg actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg
     8221 cagagatccc cgggctgcat gcccgtttat ttctggcgac attctttatg gattgccggg
     8281 cgcattgcgc ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg
     8341 ctgataattc ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat gccggtatta
     8401 atggcataac tttgccccgc gacgaggtgg taatcctgac cattaatcgt cagaacaatt
     8461 tcaccttcca gtactgtgcc tatttcctca ccctgatgct taattctttc cccagtggtt
     8521 gtgcccggct ggtacgtttc aaagatcatc gccagcgtgc gattcgggtt accgttatga
     8581 accagcttca ttgacacacc ctgactgccc atctcaatta agtcgtcctg attaatgacg
     8641 acctgcggct catcaggttt ttccggctcg gaaaagaatt ccgagagtga caaaccatac
     8701 accttcagca gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta
     8761 ctgatagcac tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc
     8821 tgctggcgga tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca
     8881 tttccttaaa ctgtagtggt cagagtcgct gtttttcagc gatatggtgc tgccaagcgg
     8941 tgataaagcc ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt
     9001 gccactgtac gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat
     9061 ccggcgagcg agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgtccatgta
     9121 gagagtttac ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt
     9181 caacctgaac ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc
     9241 gatgttccag caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca
     9301 ccaccagttc ttgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg
     9361 cattaattat cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt
     9421 tttcaccata taggtgcggc tgcacattgc tgggactact aggaagataa atgccatcga
     9481 gtttcggcaa aagttgttca agtaacgacg gttccgccag cgcatgtggt agcgcaattg
     9541 gcaagccgcc tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat
     9601 gacccttaag cctgttcctg cacattacga caccgataac cggattgttc attatatttt
     9661 ccatgctcaa tctcacaaag tggactaaat tatcgccatt actgcgcttt aaatgacctt
     9721 tatgttcaat attttttcaa tctagcagtg gattttcatt tttgcaaact caatttaaca
     9781 tttgacaaac atttggtttg catacagatt cgaatgatgg tcattatatt ttacgctttg
     9841 ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag
     9901 agaactttgt tcagcagtca gaagagaggc ggggtagcac ctttacgcag gaagtgaaac
     9961 gctacctgga gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg
    10021 gttgcttccg tggaaaacgt atccctgttt caagcctgaa gaagctcgag aaagggtgtt
    10081 atttcccggc ctcggtattt gcaatggata ttctgggcaa cgtagtagaa gaagcaggtc
    10141 tgggtcagga aatgggcgag ccggatcgta cctgtgttcc tgttctcggt tccttaactc
    10201 cttctgccgc cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg
    10261 gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctgcgcc
    10321 agcgcggatt gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgccagc
    10381 gcgacgctga aggggatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    10441 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    10501 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    10561 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    10621 tggcactaaa acgcctagtg cgtctgatgg cagaaaagca taagatgcac gccactttta
    10681 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    10741 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    10801 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    10861 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    10921 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggtga ccgtcataat caccgcgtgg
    10981 aatatcacgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    11041 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    11101 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    11161 agaatgatca cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta
    11221 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    11281 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    11341 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    11401 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    11461 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    11521 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    11581 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    11641 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    11701 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    11761 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    11821 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    11881 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    11941 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    12001 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    12061 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    12121 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    12181 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    12241 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    12301 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    12361 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    12421 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    12481 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    12541 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    12601 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    12661 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    12721 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    12781 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    12841 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    12901 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    12961 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    13021 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    13081 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    13141 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    13201 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    13261 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    13321 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    13381 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    13441 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    13501 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    13561 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    13621 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    13681 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    13741 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    13801 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    13861 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgcctgaac
    13921 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    13981 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    14041 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    14101 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    14161 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    14221 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    14281 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    14341 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    14401 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    14461 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    14521 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    14581 acgccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    14641 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    14701 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    14761 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    14821 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    14881 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    14941 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    15001 acccggacag tttcttccgt ccgttactga gctgcgcggc aattcattca cagaccaacc
    15061 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    15121 agctggcagc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    15181 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    15241 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    15301 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    15361 gttcctgctg accttcgttg gcttcagctt gagctatttc actccacacg cgccgttaca
    15421 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaat ggcctgatcc actgggattt
    15481 tggtgtttcc agcattaacg gtcaaccaat tgccgaacag ttaaaagagc tcttcccggc
    15541 gacgatagaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    15601 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    15661 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacactgt tttgttcact
    15721 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    15781 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    15841 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    15901 tgaagtgatc cgcctgatgc gtatcagcac tattgaagtg tatgaccaga actatgtaaa
    15961 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    16021 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    16081 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    16141 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    16201 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    16261 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    16321 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcgggac
    16381 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    16441 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    16501 ggactgacga cctcggacgt gatgttttaa gccgtttact gagcggagct gcgccaaccg
    16561 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    16621 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    16681 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    16741 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    16801 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    16861 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    16921 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    16981 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    17041 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    17101 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    17161 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    17221 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    17281 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    17341 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    17401 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    17461 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    17521 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    17581 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    17641 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    17701 tgacgaaccg acaaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    17761 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    17821 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    17881 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    17941 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatactctac ccggcgctat
    18001 cccattgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    18061 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    18121 tttcccgctg aacatggaga aaaagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    18181 taagtaaaac gttccgctac cggaccggtt ggtttcgtcg tcagaccgta gaagcggtaa
    18241 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    18301 cgggtaaatc cacactggca aaaatgctgg cgggaatgat agaacctacc agcggcgagt
    18361 tattgattga cgatcatccg ctgcattttg gcgattattc cttccgtagt cagcgtattc
    18421 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcacaaattc
    18481 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    18541 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    18601 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag
    18661 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc aatgcgttcg cagttgatta
    18721 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    18781 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    18841 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    18901 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    18961 ctgaatcctc cttccagatt attcacctca ttctgacaag agattaaatc ttgtcagaat
    19021 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    19081 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    19141 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    19201 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    19261 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    19321 gaggcggtgc tggagattat tctgcaggaa ctgaaccgcc ggtcaccctc accctgaaag
    19381 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    19441 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    19501 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    19561 gagagggtta gggtaagggg aaaagcgccc cccctgccgc agcctgctcc ggtcggacct
    19621 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    19681 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    19741 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc
    19801 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    19861 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    19921 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    19981 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    20041 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    20101 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    20161 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    20221 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    20281 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg
    20341 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    20401 agttactatt gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg
    20461 tatctccggt gaagtagtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    20521 cgaactgaaa taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgttatctct
    20581 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    20641 caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc
    20701 aaaagccact ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg
    20761 gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag
    20821 acgctggccc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc
    20881 cctgaccagc ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg
    20941 gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga
    21001 cttacgccgc tccccggacc tgattcctcc agggaaagta atcatcggtt ggcacaaatc
    21061 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    21121 catctgaatg acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt
    21181 tttattggct ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta
    21241 cgctggttac ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa
    21301 tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt
    21361 tcaactgcag aactttgcca actggccgaa acacactctc tccaccctat ttcactttcc
    21421 cttctcgaaa agttgttaaa cagcacacgg gtcaatgcac agccggataa tcagcttgcg
    21481 gcgttgttat gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca
    21541 tcaaatgaat ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga
    21601 tgggctgacc gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga
    21661 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    21721 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    21781 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    21841 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    21901 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    21961 aaaaggcttt ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca
    22021 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    22081 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    22141 tcagggcaaa aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat
    22201 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    22261 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    22321 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    22381 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    22441 agatctgacc tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga
    22501 cgcccttttc gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga
    22561 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    22621 caccaactat ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct
    22681 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    22741 tgatggcacc actgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    22801 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag
    22861 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    22921 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    22981 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    23041 agaagcgatg attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt
    23101 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    23161 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    23221 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    23281 cttccagtca tttgctgaaa ttagctctga acccggtcct cactttggcc tcggtctgga
    23341 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    23401 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    23461 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    23521 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    23581 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    23641 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    23701 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    23761 agtccgcatg gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    23821 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    23881 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    23941 tataaaaaac aatgcgttta gccgccgtta atttcatgta agtcatgttg ttccttttat
    24001 ttaaccgcgg actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc
    24061 acaacgttgt acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc
    24121 gatgctttgc gcttttctac caccaaaacc acagaacctg atcgtacatt gcagttatct
    24181 gccgaacagg ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt
    24241 ctgaccgtcg atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg
    24301 gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca
    24361 cacgggcttt cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc
    24421 gacagccggg ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa
    24481 gaacacgacg tcattgggta aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg
    24541 gcatccaggc gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg
    24601 tggataccaa cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc
    24661 ggcagtggca aaaacgagat ttttttaatc tgttccggta ccgacattac cgaagagcgc
    24721 cgcactcagg agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat
    24781 cgtaacgcaa tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt
    24841 ggggttgtgt atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcatttg
    24901 tttggtgacc agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac
    24961 caggtgttgg cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa
    25021 agcgcgctgg aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt
    25081 ggtttaattg aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt
    25141 tcggacagcg cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc
    25201 ggacgaggac aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc
    25261 tggctggata ccaacttgcg taaagcactg gaaaacgatc agttggttat cgaaaatcac
    25321 ctagcgtggc gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg
    25381 gttgattcca ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt
    25441 aggccgttgg gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat
    25501 aaacctgcga gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac
    25561 cgccctgaaa caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact
    25621 gacagagagt tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag
    25681 ccaactaggt gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca
    25741 actggcgcgc tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca
    25801 caaacaacct gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa
    25861 tcttcaggtg atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa
    25921 cgggatcaat gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga
    25981 acgctggtat aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct
    26041 atatcccgtc attatatatc gtattcctgg atgataatta gccgttgctg ttaaacactt
    26101 ctcttttagc ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt
    26161 gatgtctgaa tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct
    26221 tgtggcaggc gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt
    26281 gcgccaggtt gctgaatcta cgccagacat cccgaagaca ccgtgggaga tttaacaatc
    26341 gtgccttcag gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat
    26401 tataacgtta taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc
    26461 attgaccagg gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt
    26521 accattcgcc aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc
    26581 tttgcagttt cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg
    26641 aagcgtgaac tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc
    26701 gaaaatggca gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc
    26761 actatcatca cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc
    26821 attttgctcg gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc
    26881 cagtgcatcc agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct
    26941 gaaactggat tcaccggtcg cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa
    27001 aaagagtgcg aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac
    27061 tccatcggtc ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc
    27121 gatctgatgc atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt
    27181 cccttctcta cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt
    27241 gataattaag actatttacc tgttattaac actctcaaga tataaaatta ttatcagcga
    27301 tataacagga agtaattatc acctgcgtga tataaccctg cgcgcgagca gatttcacgg
    27361 aataatttca ccaggcttat tcttagctat tatagttata gagagcttac ttccgtgaat
    27421 cataaattca ggagagagta ttatgtttgt aacgagcaaa aaaatgaccg cggctgttct
    27481 ggcaattact ttggcaatgt ctctgagtgc ctgttctaac tggtctaaac gggaccgcaa
    27541 caccgcaatc ggcgcgggtg caggggcatt aggcggtgca gtactgaccg atggcagtac
    27601 gttgggtaca ttaggtggtg cagctgtcgg tggtgttatt ggtcaccagg tcggtaaata
    27661 atcgtaaata aatgtcgatt atcgactcgc aattataata cgcttgattc tgttcagtac
    27721 gaaataactg aataatacac acagtaaaat attcgaaagc cacggatata tccgtggctt
    27781 tttatgttaa ccgcctgcga gttttacctt catccctttc gcttcaagta gtgattttaa
    27841 taaatcacgc ttatcgccct ggatttcaat aactccatct ttaactgctc cgccgcagcc
    27901 gcattttttc ttcagttccg ctgcaagttt tgtcagttcg gcatcatcga gatcgatacc
    27961 ggtaatcagg caaacgccct tacctttacg tccactggtc tgacgctgaa tacgcaccgc
    28021 tccatcgcct ttagggcgta ctggggccgc tttgggctca tcaatacgcc cggtttccgt
    28081 tgagtagacc agacggctgt tggaatcact catcatgcac tccgctgtaa agaggcgttg
    28141 atcgctttca gtgtctgcgc tggatctacc gattgcgtta ccgggcgacc aatcaccata
    28201 taatcaacac cagccgccaa cgcctgttct ggcgtcataa tgcggcgctg gtcaccagct
    28261 tcactcccct gcggacgaat gcccggtgta accagtttga actcctgacc gaatacctgt
    28321 ttaaagcgca cagcttcctg agcagaacac accacaccat caaggtcaca tttttgcgtc
    28381 agtgctgcca gacgttctgc ataatctgca ggtgacagtg tcacgccaag atcggccagg
    28441 tcgctggctt ccatgctggt caacactgtc acagcaatca aaagcggtgc atctttgcca
    28501 aacggaacca gtgcctcacg cgctgcggtc atcatacgcg ccccaccgga ggcatgaaca
    28561 ttcaccatcc acacgcctaa gtcagctgca gcagcgacag cgtgcgctgc agtgttgggg
    28621 atatcgtgga atttcaggtc aagaaagata tcaaaaccac gctgttgaag ttcgcgcaca
    28681 aactgtggcc caaacaatgt aaacatctct ttaccgacct tcagacgaca atcgcgtggg
    28741 tcgatcttgt cgacaaagga cagcgcgtca tcacaattat gataatcaag ggcaacaacc
    28801 acaggagaat tcgtaacggc gcgggaagaa gatgaagcag ttaacgtcat gaccagacct
    28861 tcttgatgat gggcaccggg agtggcgcga aacaggtaaa cggcgagcat tctacccgtg
    28921 aaaataaaaa attaacagtt gcgatttttc ccctagcagg ctttttctgc tccgtcgttg
    28981 ccggagcaca atgggacatc aataactagc atgttgtaac taaagtcgga tttttttaaa
    29041 aattacaggc catcaagacc gcgaatcggt ttaatagttg accaggcacg acaagacgga
    29101 caatgccagt agagggtgta tgcagtaaaa ccacatttct ggcagctata acgaggctta
    29161 ctacgcacct tctcgccaac catgtcacgc agcaccatca gactctcttt ggcgcgccct
    29221 tcttccgctt catttaagtg gtaatccatt agcttatgga atacacgcat ggtcggatga
    29281 cgctgaagct ggcgtgtaat atagacctgt gcagcctcac taccgtcgcg cgcttcgatg
    29341 atatcagcaa gcatcaattc agcatcggca ccggtgttct cttccaccgc gcgttgcagg
    29401 aattctgccc attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc
    29461 gtttcgctga ccagttctct gtcctgggat atgacgcgtt gcaaactttc gacggctttg
    29521 gcgtattctc ctttcgccat aaacacgcgt cccatcatga tggatacgcg ggcgctgttt
    29581 ttatctgccg ccgccccttt ttttagcaag gtcatggcac gatcgagatc gtcgctggcc
    29641 atatgctgca gggctaactc acagtagaaa tgggcaattt cgacgcgctg tttatcttta
    29701 cccagcttca ccaggcgttc ggcaacatca attgctttct gccactcact ggtagcctgg
    29761 tagatttgta gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga
    29821 ttgaacatgt cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg ccccagttgt
    29881 tgaatcgcca acagacgctg ttcataggtc agcgaggcgc tttccattag ggtctgatgg
    29941 atgcgaatag cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag cgtaaggtgg
    30001 gcttcaacgg tgcctgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc
    30061 tgttgattac taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct
    30121 tcatcttgct tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct
    30181 acaggcaaaa gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgag
    30241 tgcggcacta cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta
    30301 cgttcagcgc gcgccaggga aacacgaact cgcagccaga acaggccaca aatcaaccaa
    30361 ccgatagcaa accccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct
    30421 tgcgctaaca gataattaaa cgtcacctgt tgatcgttct gcgcacccaa cgtgaccgaa
    30481 atcacgaaga tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt
    30541 atgtggttag agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt
    30601 ttcgcgccgt ctgcgaaatg gcatccacgc gcattgttga tgatttaagg cctaaattcg
    30661 gaaaatcaac cagcgttaac tttcttgttc tcgttgtgca atttcgcgat tttcttccgc
    30721 aggtggtgtt aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc
    30781 ccacgaaatc aacgtagcta ccaccagatc gcgtggccaa tgcatcccga gcagcaggcg
    30841 gcttcccatg actcccgttg cccagaccaa caagatagca atggttaacg ttcgccgacg
    30901 cggccacagc aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg tgtgaccgga
    30961 aggaaaggca aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg gaatattttt
    31021 ctcttcagcc aactgttctt ttactagatt tccgcgttct gctcgcttta aagtgtagaa
    31081 ctcatcaacc ggaatatgat gtgttttttc caaccagata acaaaaggtc gtggttcctg
    31141 tactttgtct ttgatccagg atttaacgcc ttgtccaaca aggattgccg ccgccagaat
    31201 ggcaaataat acaaaggcag ccttaatgcg aaaacgcaga caccagagaa accagccgaa
    31261 caaaatcaaa tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa cccaaaaagc
    31321 cgcttttagt agccaacttt gttctccagg ttgccaacgc cagccagaaa tccatacggc
    31381 tactggcatg acaagcaata gtgcagctcc cactgcggta cgtcgggcaa tcgaacgcat
    31441 ggcctctcct ttttgataag tcccacaatc ataactgaaa atgccagttc caagaaaaat
    31501 tgacagattt gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga accccttctc
    31561 gttatggcaa aataagccaa tacagaacca gcattatctg gagaatttca tgcagcttaa
    31621 acgtgtggca gaagccaaac tgccaacccc atggggcgat ttcctgatgg tgggatttga
    31681 agaactggca accggacacg atcatgtcgc gctagtctat ggcgatattt ccgggcatac
    31741 cccggtactt gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt tcagcttgcg
    31801 ctgcgattgt ggcttccagc tcgaagcggc attgacgcaa attgccgagg aaggccgtgg
    31861 tattttgctg tatcaccgtc aggaaggtcg taacattggt ctgctgaata aaatccgcgc
    31921 ttacgcactg caggatcaag gttacgatac cgtagaggct aaccaccagt taggcttcgc
    31981 cgctgatgag cgcgacttca ctctttgcgc tgatatgttc aaactccttg gcgtcaatga
    32041 agtccgcttg ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag cagggattaa
    32101 tattgttgaa cgcgtaccat tgattgtagg tcgtaacccc aataacgaac attatctcga
    32161 taccaaagcc gagaaaatgg gccatttgct gaacaaataa ccctcttgca tcgtgtaatt
    32221 catttgcttg ccggaagcaa aataaccggc aagcaaatac ttgttacttc aacatattac
    32281 gaatgacata atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc gcggtgtcga
    32341 tacgacaacg gcagggtacg acttcctggc taccatccgc gcgcgtaagc gtcaccggaa
    32401 ccgtcgcgcc aggttgtaga ttttgcagat cgccaatatc aatcttctct tccccggtta
    32461 gccctaacgt tttacgcgtc acgccttgcg gaaattccag cggcaggatg cccatgccaa
    32521 ttaaattcga acggtgaatt cgttcaaacg attcggcaat caccacacga attccaagca
    32581 gacgaggtcc ttttgccgcc cagtcacggc ttgagcctga tccatactct ttcccggcaa
    32641 tcaccgccag cggcgtttgc tcctgcttat agcgcatcgc agcatcataa atagagacta
    32701 cgtcgctgtc aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc atttcattac
    32761 ggatgcgaat attggcgaag gtgccgcgca tcatcacttc atggttacca cgccgcgaac
    32821 cgtaggagtt aaagtctttt cgctcaacac cccgaccttg tagatatcga cccgctgggc
    32881 tgtcgggctt aatactgccc gccggagaga tatggtcagt ggtgactgaa tcccccagca
    32941 ttgcgaggat ccgcgcaccg tgaatatctt ccactggtgc tggtgttgcc tgcatttcat
    33001 caaagaaagg cgataagcga atataggttg agtcctcctg ccaaccgtag gtatcggatc
    33061 gtgtgacgtt aatttccttc cactctgctg tgccttcaaa aacttctgcg tactctttgc
    33121 ggaacatttc tgtggagact tgttctaccg cacgggcaat ttcttgtgcc gatggccaga
    33181 tatctttcag ataaaccggc tcgcctttgc gatcatggcc gataggctca gaagccaggt
    33241 tgatattcat atttcccgcc agcgcatagg caaccaccag cggtggcgag gccagccagt
    33301 tagttttaac cagcggatgg atacggcctt caaagttacg gttgccggac agcaccgcac
    33361 cgacggttaa atcgcctttt ttgattgccg tttcgatagg atcgggcagc ggcccagagt
    33421 taccaataca ggtggtacaa ccgtatccca caaggttaaa ccccagttcg tcgagatacg
    33481 gtgtcagttt cgcttttgcc agataatcag aaacgacttt cgaacccggt gccaacgacg
    33541 ctttgaccca cggttgccgc ttgaggccaa gagttacggc ttttttcgcc agcaagcctg
    33601 cggccatcag cacacttggg ttagaggtgt tggtgcacga ggttatcgca gcaatgacca
    33661 cagcgccatc aggtaactga tactgatgtc cgttcataac ataatcgact ggcaggcgat
    33721 ctttatgcgt ggcattcact tccagttcgt tactggcggc aaatgctttt ggtacatcgg
    33781 gcagtgcaac gcgatcctgt gggcgttttg gccctgccag gctcgcttca acgtcattca
    33841 tatccagttc taacatactg gtaaaaatcg gttcatcgcc cgggttacgc cacatgccct
    33901 gcgctttggc atatttttcg accaactcga cctgatcttc gctgcgcccg cttaaacgca
    33961 tgtaatcgag ggttactgca tcgattggga agaagccaca ggtggcacca tattctggcg
    34021 acatattggc aatggtggcg cgatccgcca acggtagtga atccagacca tcaccataaa
    34081 attcgacgaa tttccccacc acgccatgtt tgcgcagcat ttgggtaaca gtgagaacca
    34141 ggtctgtggc ggtaatacct tcacgtaatt ttccggtaag tttaaagccc actacgtccg
    34201 ggataagcat ggaaaccggc tggcctaaca tggcggcttc tgcttcgatc ccacccacgc
    34261 cccaccccag cacgccaagg ccgttgatca tagtggtgtg cgagtcagta ccaacgagtg
    34321 tatccggata agcaatccat tcaccgtcct gcaattcgct ccacactgct ttgccgagat
    34381 attcgaggtt aacctgatgg caaatgcctg tgcctggcgg cacgacgcta aaccgactga
    34441 acgcttgctt tccccatttc aggaacacat aacgttcgtg gttgcgctcc atttccaggc
    34501 gtacgttttc ttcaaatgcc tcatcatcac caaaacgatc gacggtcacc gagtggtcaa
    34561 tgaccaggtc gaccggtgag agcgggttaa cctttgcagt atcgccaccg aggcgtttaa
    34621 ccgcttcgcg cattgccgcc agatcaacaa cggcaggtac gccggtaaag tcctgcatca
    34681 gcacccttgc cgggcggtag gcaatttcac ggtcagcatg ggcatttttc agccatcccg
    34741 ccagtgcgtg gatatcctct tcggtaactg agttaccatc ctgccagcgc agcaggtttt
    34801 cgagcaaaac tttgagtgac ttgggtagac gggtgatatc gcccagtgat ttagcagcaa
    34861 gcggcaggct gtagtagtgg taagttttat ctttggcctg caacgtgtcc ttactggctt
    34921 ctcgtagggt tgacgacata gctcctcctt aatgacaggg ttgcgaagcc ctgattctct
    34981 tcagggtgaa tattaaagat aacacaaaca tcgcgtaacg atttgataac aacccaaatt
    35041 gataaaagag gagaaacctt gtggcaaagc aaatgacaac cccgccgcag cggggtcaag
    35101 gatcagtgag taatgatcca gaggagttcg gcccagaaga gcatcgacag agaaaacgcg
    35161 cccatccagg accaatattt catatttgtg ttgatcatag gctacaccct taacggttat
    35221 tattttgtgt tgagataact caagctgtta ttgctgcggt gtattaaaag ccctttaatt
    35281 cattaaaaat gggtaataag aatgatgata attattgttt cggctgatca tcttcagcaa
    35341 acagttctat aaaggcttgc tgctgcgatg ttagttgcca gtcgtctgga ataacgacgg
    35401 aacattcccg tttcgtttct ggttcattgt cactatgctg gcataatggt gacgactcca
    35461 gttgctcctg accgaccata gttatcccca cactttccaa atcagcagtg cgacaaccac
    35521 ccagaaaagt gcagaaccaa gaaaaaccgc cagccaggct ttacgtttta attcaggatc
    35581 acggcggggt tcctgatttc cagaaggcat tgctaacctc atataatcgc catcacttat
    35641 cagcaagacg ttgaatgatt ggtgtaatta atcatacgat gagacaaatc ctaaagaaac
    35701 tttagtcaaa aatccagcga atttacgaat cgtctccagt gaataattca atcttttgac
    35761 ctgaaataga tatttgaaaa ctggaatttg cgaggcgggt aattagacac tactttacag
    35821 gtaatttacc ctataaatat gagggataaa aatattgtgc acggtataga aataagtaac
    35881 aaaagagaat atattccggc accttcgcta cataaaaggt gccgaaaata acgcaagaaa
    35941 ttatttttcc ggcagtttta tatctttaaa catgacctca atttcttcat tggagcgcaa
    36001 tgcgacagcc gcatcaacga catcacgcgt taaatgcggt gcaaaacgct gaatgaaatc
    36061 atacatataa ctgcgcaaaa aagtactacg gcgaaaacca attttggttg tactgtggct
    36121 gaagatatcg tgagcatcaa cgcgcacaag gtcgggatcg gcgaccggat ccaccgccat
    36181 gctggcaatg acccctaccc ccagccctaa ccggacgtaa gttttaatga cgtcagcatc
    36241 cgttgccgtg aaaacgatac gcggcgttaa ccctgcgcgg ttaaaggcag tatccagttc
    36301 tgaacgtccg gtaaagccga aggtatatgt caccaacgga tattgcgcca gttcttcaat
    36361 ggtaatggct tttttgcctg ccagcgggtg atccggagtg actacaatag cccgattcca
    36421 gtggtagcac ggtaacatca ctaaatcttc atacagatgc agcgcttctg tggcgatagc
    36481 gaaatcggca ttgcctttag agacggcatc agcaatttgt gtcggcgggc cctggtgcat
    36541 atgcaaagaa acgcggggat aacgctcaat aaagcctttg ataacgtttg gtaacgcgta
    36601 gcgtgcctgg gtatgcgtgg tggcgatata cagcgagcct ttatccggcc aggtgtgctc
    36661 accggcaacc gattttatgg catcgacttt cgacaggact tcgcgagcga tacgaattat
    36721 ttcttgtcct gctggcgtta cctgcgtcag gtgcttgccg cttcgggaaa aaatttgaat
    36781 gcctagctcg tcttccagca ttctgacttg tttactgatc ccgggttgtg atgtgtaaag
    36841 tccttccgct gttgatgaga cattcaggtt atgattgacc acctcaacaa tatagcgaag
    36901 ttgttgtaat ttcatgttaa accatccact tagacgccat cagaacatcg ttttttcgtc
    36961 acacctggcg ggtgactaat acgatttaat aataaaaaag gtgctaacta taaccactat
    37021 atcatttata gccttagtgt ataggtgtga agaaaaaata atatgcaata aaaaagggcc
    37081 gctttcgcga ccctttgttt ataaaaacct gacagaatta aaggttattt ttttccttca
    37141 acccatttgc catcaacata aaatgctgac cagccagtcg cctttccgtc tttttccgaa
    37201 gagacatatt gctgtttggt tttacggcta aagcgaacca tggtcttatt accttccgga
    37261 tcctgctgtg gcgcatcggc cagataacgc agtttttccg gcagacggtc gcggaagcga
    37321 taaagctctt ccaccagtgg cgcacgcgtt tcacgcgatt tcgggaaagt gttggcagcc
    37381 aggaacacac cggcagcacc gtcacgcagc acgaaataag catctgattt ttcgcacggc
    37441 agctcaggta atggcaccgg atcttctttc ggtggtgcca cttcgccgtt acgtaaaatc
    37501 ttacgtgtgt ttttacactc ttcgttggtg caggccatgt atttaccgaa acgccccatt
    37561 ttcaggtgca tttcagagcc acatttttca cactcaacga tcgggccgtc ataaccttta
    37621 atgcggaatt cgccctcttc gatctcgtaa ccgtcgcagg ttgggttatt accacagaca
    37681 tgcaacttac gtttcggatc gatgagatag ctgtccatcg ccgtgccgca tttcgggcaa
    37741 cgacgttttg cgcgcagcgc gttggtttca gcatcttcgc cttccagcac gttcagcact
    37801 tcgttttccg gcaccaggtt aatggtggtt ttgcaacgct ctttcggcgg cagcgcatag
    37861 ccagaacagc caaggaatac cccggtactc gctgtgcgaa tccccatttt gcgaccacaa
    37921 gtcgggcagt caatgctggt cagaaccatc tggttcgggc gcataccacc ctcttccgga
    37981 tctttttcag ctttatctaa ctgctgggtg aaatccgaga agaagtgatc cagtacagct
    38041 ttccactctg cttcgtgatt tgccacctgg tcgaggctgt tttccatctg cgcggtaaag
    38101 tcgtagttca ttaactcgcg gaaattttct tcaaggcgat cggtgacgat ttcgcccatt
    38161 ttttccgcat agaaacgacg attttctact cgcacatagc cacgatcctg aatggtcgaa
    38221 atgatcgacg cataggtaga cggacgaccg ataccacgtt tttccagttc tttaaccagc
    38281 gatgcttcac tgaaacgggc tggcggcttg gtaaagtgct gggctggtgt tagttcaacg
    38341 agcgtcagag catcgccttt atcgactgcc ggtaagatac gatcttcatc gcctttacgc
    38401 agtgcaggca tcacttttgt ccagccatca aagcacaaaa tacgaccgcg tgctttcagg
    38461 cggaaatcgc ccgcaccaac ggtcagcgtc gtggagtcat atttcgctgg ggtcatctgg
    38521 caggcaacga actgacgcca gattaactgg tacagtttct gcgcatctgc ttccatatcc
    38581 ttcagcgatt ccgccatcac attgacgtca gaaggacgaa tcgcttcgtg cgcttcctgt
    38641 gagttttctt tgctggcgta ctgattcgga ctttccggca gatatttctt accaaaatta
    38701 tcgctgatat aaccgcgaac catatttacc gcgtcctgac tcaggttagt ggagtcggta
    38761 cgcatgtaag tgatatagcc tgcttcatac aaacgctgcg ccatcatcat ggtttttttc
    38821 acgccaaaac caagacgggt gctggcagct tgttgcagcg tagaggtaat aaaaggagcg
    38881 ccaggtttac tggttgtcgg tttgtcttca cgttccagca cgctgtagcg cgctttttcc
    38941 agcagactta ccgcagcctg agtttgttct ttgttgaccg gacggaacgg tttgtcattc
    39001 tgatgagtca cctgcaacgc caacgcttca ccagatggcg tagtcgtgct ggcatcgact
    39061 tcccagaact cttccggtac gaacgcttta atttcacgct cacgctctac caccaggcgg
    39121 accgccaccg actgcacacg accggcagac agaccacgag cgatcttttt ccacagcagc
    39181 ggcgaaacca tataccccac cacgcggtcc ataaagcgac gcgcctgctg ggcattaaca
    39241 cgatcaatat tcagctcacc cggtttgtta aatgcctggc ggatcgcgtt tttagtaatt
    39301 tcgttaaaca ccactcggct atagcgcgca tcatcaccac caatcacttc ccgcaggtgc
    39361 catgcaatgg cttccccttc gcggtcaagg tcggttgcga gatagatgtg gtcggctttt
    39421 tcagccagtt gtttcagttc agagacgacc ttctctttac caggcaacac ttcatagtgc
    39481 gcctcccaat tgtgccacgg gtcaaccccc atacggttga cgagagcgcc acgttcatcc
    39541 tttttaggct ttttagccgt cttggtggag gtagagtcgg cactcttttt ggcagctgag
    39601 ccactggtcg gcaaatcgcg gatgtgaccg acgctggatt tcaccacgta gtcacaaccc
    39661 agatacttgt tgatcgtttt ggcttttgcc ggggactcaa cgatgacaag agctttaccc
    39721 atattcacct tacctaattt gattcttcca ggaatgcgtc gcacgttgat aaaccttcca
    39781 ctggcaacga gttaccgata tggactcgct ctctgcggat atcaacccct tgtcgtgact
    39841 ctgttcacga aaattcaggt gactgagtta acaggtcttg gcctacaggc gctatagcac
    39901 gacggaattt tggtcgaatg tcaagcaaat ctattgccag attgacgaaa gcgtcacact
    39961 gtacctgata aaatccgata cgcaacttta ttagcatgca aaatccaaag ttgccagtaa
    40021 ggcgctcttg ccaccagcgt ggtatatttg tctgcaagcc atgcctggcg tagactaatg
    40081 tccttgttta aggagtaaat aatgaacaaa gagacacaac ccatcgaccg cgaaacgctg
    40141 ttgaaagagg ccaacaaaat cattcgtgag catgaggata cgctcgcagg gattgaagcc
    40201 accggcgtca cgcagcgtaa tggcgtgctg gtatttaccg gcgactactt tttagatgaa
    40261 caaggattgc caacagccaa aagcaccgcg gtgtttaata tgttcaaaca cctggcccat
    40321 gtgctttcag aaaaatatca tctggttgat taaagcaaaa cgcgaggtct tagcctcgcg
    40381 tttgtctttt acatcaatgg cttttgaccc cgctgccacc agcgtagcaa caatcgatcg
    40441 gcgctctctg ccgcgctgcc ggtgaatcgg tcaatgagtc gtttacgctg catatagcgt
    40501 acattaacca cttcacggcc ttccatcagg ctaagaataa cttcatcact ggtgttgatt
    40561 tcatcaacca ggcctttctc taccgcctgt tgtccgtacc agtgttcacc cgttgccacc
    40621 tgttcaatat ccagagacgg acgcatacgc ttcacaaaat ctttaaataa ctgatgcgtt
    40681 tcgttcagct cttcgcggaa tttctcccgc ccttcttcgg tattttcacc cagcaaggtc
    40741 agcgtgcgct tatactgccc ggcggtgtgc agttcgatat caatatcttt gcttttcagg
    40801 aagcggttaa agttgggcat ttgcgccacc accccaatgg aacccacaat agcaaacggt
    40861 gcggaaacaa ttttgtccgc cacacaggcc atcatgtaac cgccgctggc agcgactttg
    40921 tctaccgtaa cagttaaagg aatgttttta tcacgcagac gctgcagctg cgaagccgcc
    40981 aacccgtaac catgcaccat gccaccaggg ctttccagac ggagcacaac ctgatcctgc
    41041 ggtttgaatg ctgcgagtac cgccgttatc tcttcacgta gcgagttcac ttcatgggcg
    41101 tccatgctgc ctttaaaatc cagcacccag acgcgaggtt tactgtcagt tgccacctcg
    41161 cccagtttgg ctttcgcttt tgctgctttc gcttcttgct tgtgcttttt cttctgcgct
    41221 ttgtgccact gtttttgctg atgtgagtcc atcagcgccg cggccagttc ttctttcatc
    41281 tccttatact gttcgctgag attgttgacc cgtaactcgc cacgctggcg tttattacgt
    41341 tgagcaacat tgacaataat ggcggcaatc gccgcaatcg ctagcacaac ggtaacgatt
    41401 ttagccaaaa acaaaccata ttcagacaac aattccacga gtccccacct tggttaaaca
    41461 acgctttggc tcacagttta caacaggcat gttaagccgt ctcgcagaga tgttattgca
    41521 gcgaaagtat gacaaagtat ccattttgcg gtgttatcgc cccaggctga ttgaaatgcc
    41581 cttctgtttc aggcataaag ccccaaagtc ataaagtaca ctggcagcgc gacagacaac
    41641 gtcgttgagg agtagccatg cattaccagc caaaacaaga tttactcaat gatcgcatta
    41701 tcctggtgac gggagccagc gatgggattg gtcgtgaagc cgcgatgacg tatgcacgct
    41761 atggtgcgac agtgattctg ttgggccgta atgaagaaaa attacgtcag gtagccagcc
    41821 acataaacga agaaactggg cgtcagccac agtggtttat tctcgatttg ctgacctgca
    41881 cgtccgaaaa ttgccaacaa ctggcacagc gcattgtcgt taattatccg cgtctggatg
    41941 gtgttttgca taatgccgga ttgctcggcg atgtttgccc aatgagcgaa caaaatccgc
    42001 aggtctggca ggacgtcatg caggtcaacg ttaatgccac ctttatgctc acccaggcac
    42061 tgcttccttt attactcaaa tcggacgccg gttcactggt ctttacttca tcaagcgttg
    42121 gacgtcaggg acgagccaac tggggtgcat atgcagcgtc gaaatttgcc accgaaggga
    42181 tgatgcaggt actggccgat gaatatcagc agcgcctgcg cgtcaactgc attaacccag
    42241 gcggtacgcg caccgcaatg cgcgcaagcg ccttcccgac cgaagatcca cagaaactta
    42301 aaacacccgc tgatatcatg ccgctctacc tctggctgat gggcgatgac agccgccgta
    42361 aaaccggcat gacctttgac gcccaaccgg gccgtaaacc aggaatttcc caatgagtga
    42421 tgaacgctac caacagcgtc agcagcgagt gaaagaaaaa gtagatgctc gtgtggccca
    42481 ggcccaggat gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac
    42541 cgcggcattt ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca
    42601 gtttattaaa ggcacctggc ctaatggcga acgcaatctg ctggagccac atggcgttga
    42661 gtttcaggtg atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc
    42721 cgcctgccgc gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat
    42781 ggttttgctt gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt
    42841 ggtgcaggcg ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg
    42901 tcatcgggat attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcagacatgc
    42961 gtttgatgcc ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga
    43021 tatgacgtcc ggcatacttt gattaaccgt tattacgccc gccagaacga cgactgccgc
    43081 tcacctgact gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt
    43141 ctttttctac agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat
    43201 agttagtctg ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg
    43261 ggatatcacc gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca
    43321 gacgacgaac ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc
    43381 cttcgccgcc gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc
    43441 cacgactcaa atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt
    43501 cacgttcaac ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga
    43561 acagcagcag accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac
    43621 gcagctttgg cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca
    43681 actcaccttc cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta
    43741 ccgaaatcag atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc
    43801 cgagtttagc aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt
    43861 cacggcggga gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc
    43921 ttccttttgg tgtcgccttc acaggcgtcg aacaggagaa tcaatggcgc cgtatagcgc
    43981 cattttaagg ccgcgtagta tacagagtta cgcacttata agaaaggctt cacatcacct
    44041 acgccttcac gcaccacgac cggcgtatca tcggtaagat caataaccgt tgttggtttc
    44101 tgcccgagat agccgccgtg aataatcaaa tctacctgtt tttccagacg gtctttaatt
    44161 tcttcgggat cggattcggt aaattcgctg cctggcagca ttagcgaagt ggaaagcatc
    44221 ggttcgccca gtgcttcaag caacgcctgc gcaatcgggt tagaaggcac acgcataccg
    44281 atggttttgc gtttctcctg caacagacgg cgtggcactt ccttcgtccc cttcaggatg
    44341 aaggtatagt tgcccggcgt gttgtttttc atcaaacgaa acgcaacgtt atccacgaat
    44401 gaataggtcg agagttcaga aagatcgcga cacatcaggg taaagttgtg accgtccggc
    44461 agctggcgaa tacgacaaat acgctccatg gcgtttttat cttcaatttt acagccgagc
    44521 gcatagccgg aatcagttgg atagacaatc accccgcctt tacgcacgat ctccaccgcc
    44581 tggttgatca gacgttgctg cgggttatca ggatgaatat aaaaaaactg gctcataatt
    44641 ccctctctgt ggtgttctgc ggctgttccc atagctgcca gacgccttca acgcctgcgg
    44701 gcagccagag tttacgaccc agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt
    44761 gtgatgccca taaatgatgc tgacgcgcaa gtgtggccag ctgggtgcgt tcattgggcg
    44821 attgctggca ctgcgcgact tccatcgcgt cgccgtggtg ttcggcaaaa tgcgcaacca
    44881 atcttttcag ccatttagca ctaagattgt accgcccagg atgagctagc accgccttac
    44941 cgccagaatg atgaatgaca tcaatagctt gttctattgt acaccactgt ggcggaacgt
    45001 atccggtttt cccgcgcgcc agatactttt taaagacatc tgccattgaa ctggctttgc
    45061 cgcactccac aaggaaacgc gcaaaatgac cgcgcgtcac tgcgccgccc tgcgccagtc
    45121 gttgtgcgcc ttccagcgcc ccaggtattt gcgctttttc aagtctttcg gcaatttgct
    45181 gcgcccgctg attgcgccgt tctgtctgct gcgcgaggaa ctcacacatt agcggatgag
    45241 taatatcaat attcagcccg acaatatgaa tttcatgatt ttcccagacc gtggaaattt
    45301 ccacgccggg aataagattc agcgccagtc ccgaacgtga aatttcctct cttgctggcg
    45361 cgattgcagc tgtggtgtca tggtcggtga tcgccagggt gccgacgcgc atctcgactg
    45421 cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca
    45481 ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt
    45541 ttttgcgccg acatcataac gggtctggca aatattctga aatgagctgt tgacaattaa
    45601 tcatcgaacc agttaactag tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca
    45661 attttcgtac tgaaaggttg gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg
    45721 taaagcaatc agatacccag cccgcctaat gagcgggctt ttttttgaac aaaattagag
    45781 aataacaatg caaacacaaa aaccgactct cgaactgcta acctgcgaag gcgcttatcg
    45841 cgacaacccg actgcgcttt ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct
    45901 ggaatccgca gatatcgaca gcaaagatga tttaaaaagc ctgctgctga tagacagtgc
    45961 gctgcgcatt acagcattag gtgacaccgt cacaattcag gcactttccg gcaatggcga
    46021 agccctgctg gcactactgg ataacgccct gcctgcgggt gtggaaagtg aacaatcacc
    46081 aaactgccgt gtgctgcgct tcccccctgt cagtccactg ctggatgaag acgcccgctt
    46141 atgctccctt tcggtttttg acgctttccg tttattgcag aatctgttga atgtaccgaa
    46201 ggaagaacga gaagcaatgt tcttcggcgg cctgttctct tatgaccttg tggcgggatt
    46261 tgaagattta ccgcaactgt cagcggaaaa taactgccct gatttctgtt tttatctcgc
    46321 tgaaacgctg atggtgattg accatcagaa aaaaagcacc cgtattcagg ccagcctgtt
    46381 tgctccgaat gaagaagaaa aacaacgtct cactgctcgc ctgaacgaac tacgtcagca
    46441 actgaccgaa gccgcgccgc cgctgccagt ggtttccgtg ccgcatatgc gttgtgaatg
    46501 taatcagagc gatgaagagt tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc
    46561 tggagaaatt ttccaggtgg tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct
    46621 ggcggcctat tacgtgctga aaaagagtaa tcccagcccg tacatgtttt ttatgcagga
    46681 taatgatttc accctatttg gcgcgtcgcc ggaaagctcg ctcaagtatg atgccaccag
    46741 ccgccagatt gagatctacc cgattgccgg aacacgccca cgcggtcgtc gcgccgatgg
    46801 ttcactggac agagatctcg acagccgtat tgaactggaa atgcgtactg accataaaga
    46861 gctgtctgaa catctgatgc tggttgatct cgcccgtaat gatctggcac gcatttgcac
    46921 ccctggcagc cgctacatcg ccgatctcac caaagttgac cgttattcct atgtgatgca
    46981 cctcgtctct cgcgtagtcg gcgaactgcg tcacgatctt gacgccctgc acgcttatcg
    47041 cgcctgtatg aatatgggga cgttaagcgg tgcgccgaaa gtacgcgcta tgcagttaat
    47101 tgccgaggcg gaaggtcgtc gccgcggcag ctacggcggc gcggtaggtt atttcaccgc
    47161 gcatggcgat ctcgacacct gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc
    47221 caccgtgcaa gcgggtgctg gtgtagtcct tgattctgtt ccgcagtcgg aagccgacga
    47281 aacccgtaac aaagcccgcg ctgtactgcg cgctattgcc accgcgcatc atgcacagga
    47341 gactttctga tggctgacat tctgctgctc gataatatcg actcttttac gtacaacctg
    47401 gcagatcagt tgcgcagcaa tggtcataac gtggtgattt accgcaacca tattccggcg
    47461 cagaccttaa ttgaacgcct ggcgacgatg agcaatccgg tactgatgct ttctcctggc
    47521 cccggtgtgc cgagcgaagc cggttgtatg ccggaactcc tcacccgctt gcgtggcaag
    47581 ctgcccatta ttggcatttg cctcggacat caggcaattg tcgaagctta cgggggctat
    47641 gtcggtcagg cgggcgaaat tcttcacggt aaagcgtcga gcattgaaca tgacggtcag
    47701 gcgatgtttg ccggattaac aaacccgctg ccggtggcgc gttatcactc gctggttggc
    47761 agtaacattc cggccggttt aaccatcaac gcccatttta atggcatggt gatggcagta
    47821 cgtcacgatg cggatcgcgt ttgtggattc cagttccatc cggaatccat tctcaccacc
    47881 cagggcgctc gcctgctgga acaaacgctg gcctgggcgc agcagaaact agagccagcc
    47941 aacacgctgc aaccgattct ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa
    48001 agccaccagc tgttttcagc ggtggtgcgt ggcgagctga agccggaaca actggcggcg
    48061 gcgctggtga gcatgaaaat tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc
    48121 gcgctactgg aaaacgcagc gccgttcccg cgcccggatt atctgtttgc tgatatcgtc
    48181 ggtactggcg gtgacggcag caacagtatc aatatttcta ccgccagtgc gtttgtcgcc
    48241 gcggcctgtg ggctgaaagt ggcgaaacac ggtaaccgca gcgtctccag taaatccggc
    48301 tcgtcggatc tgctggcggc attcggtatt aatcttgata tgaacgccaa taaatcgcgc
    48361 caggcgctgg acgagttagg cgtatgtttc ctctttgcgc cgaaatatca caccggattc
    48421 cgccacgcga tgccggttcg ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg
    48481 ccattgatta acccggcgca tccgccgctg gcgttaattg gtgtttatag tccggaactg
    48541 gtgctgccga ttgccgaaac cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac
    48601 agcggcggga tggatgaagt ttcattacac gcgccgacaa tcgttgccga gctgcatgac
    48661 ggcaaaatta agagctatca attgaccgct gaagattttg gcctgactcc ctaccaccag
    48721 gagcaactgg caggcggaac acctgaagaa aaccgtgaca ttttaacacg cttgttacaa
    48781 ggtaaaggcg acgccgccca tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc
    48841 ctgcatggcc atgaagatct gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt
    48901 ggttccgctt acgacagagt taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt
    48961 ttagcgaaaa tcgtcgcaga caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg
    49021 ctggccagtt ttcagaatga ggttcagccg agcacacgac atttttatga tgcgctacag
    49081 ggtgcacgca cggcgtttat tctggagtgt aaaaaagcgt cgccgtcaaa aggcgtgatc
    49141 cgtgatgatt tcgatccggc acgcattgcc gccatttata aacattacgc ttcggcaatt
    49201 tcggtgctga ctgatgagaa atattttcag gggagctttg atttcctcct catcgtcagc
    49261 caaatcgccc cgcagccgat tttatgtaaa gacttcatta tcgaccctta ccagatctat
    49321 ctggcgcgct attaccaggc cgatgcctgc ttattaatgc tttcagtact ggatgacgac
    49381 caatatcgcc agcttgcagc cgtcgctcac agtctggaga tgggggtgct gaccgaagtc
    49441 agtaatgaag aggaactgga gcgcgccatt gcattagggg caaaggtcgt tggcatcaac
    49501 aaccgcgatc tgcgtgattt gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa
    49561 ctggggcaca acgtaacggt aatcagcgaa tccggcatca atacttacgc tcaggtgcgc
    49621 gagttaagcc acttcgctaa cggttttctg attggttcgg cgttgatggc ccatgacgat
    49681 ttgcacgccg ccgtgcgccg ggtgttgctg ggtgagaata aagtatgtgg cctgacgcgt
    49741 gggcaagatg ctaaagcagc ttatgacgcg ggcgcgattt acggtgggtt gatttttgtt
    49801 gcgacatcac cgcgttgcgt caacgttgaa caggcgcagg aagtgatggc tgcggcaccg
    49861 ttgcagtatg ttggcgtgtt ccgcaatcac gatattgccg atgtggtgga caaagctaag
    49921 gtgttatcgc tggcggcagt gcaactgcat ggtaatgaag atcagctgta tatcgatacg
    49981 ctgcgtgaag ctctgccagc acatgttgcc atctggaaag cattaagcgt cggtgaaacc
    50041 ctgcccgccc gcgagtttca gcacgttgat aaatatgttt tagacaacgg ccagggtgga
    50101 agcgggcaac gttttgactg gtcactatta aatggtcaat cgcttggcaa cgttctgctg
    50161 gcggggggct taggcgcaga taactgcgtg gaagcggcac aaaccggctg cgccggactt
    50221 gattttaatt ctgctgtaga gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg
    50281 gttttccaga cgctgcgcgc atattaagga aaggaacaat gacaacatta cttaacccct
    50341 attttggtga gtttggcggc atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc
    50401 tggaagaagc ttttgtcagc gcgcaaaaag atcctgaatt tcaagcccag ttcaacgacc
    50461 tgctgaaaaa ctatgccggg cgtccaaccg cactgaccaa atgccagaac attacagccg
    50521 ggacgaacac cacgctgtat ctcaagcgtg aagatttgct gcacggcggc gcacataaaa
    50581 ccaaccaggt gctggggcag gcgttgctgg cgaagcggat gggtaaaacc gaaatcatcg
    50641 ccgaaaccgg ggccggtcag catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc
    50701 tgaaatgccg tatttatatg ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc
    50761 gtatgcgctt aatgggtgcg gaagtgatcc cggtacatag cggttccgcc acgctgaaag
    50821 atgcctgtaa tgaggcgcta cgcgactggt ccggcagtta tgaaaccgcg cactatatgc
    50881 tgggcaccgc ggctggcccg catccttatc cgaccattgt ccgtgagttt cagcggatga
    50941 ttggcgaaga aaccaaagcg cagattctgg aaagagaagg tcgcctgccg gatgccgtta
    51001 tcgcctgtgt tggcggcggt tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa
    51061 ccaacgtcgg cctgattggt gtggagcctg gtggtcacgg tatcgaaact ggcgagcacg
    51121 gcgcaccgct aaaacatggt cgcgtgggca tttatttcgg tatgaaagcg ccgatgatgc
    51181 aaaccgaaga cgggcagatt gaagaatctt actccatctc cgccggactg gatttcccgt
    51241 ctgtcggtcc acaacacgcg tatcttaaca gcactggacg cgctgattac gtgtctatta
    51301 ccgatgatga agcccttgaa gccttcaaaa cgctgtgcct gcacgaaggg atcatcccgg
    51361 cgctggaatc ctcccacgcc ctggcccatg cgctgaaaat gatgcgcgaa aacccggata
    51421 aagagcagct actggtggtt aacctttccg gtcgcggcga taaagacatc ttcaccgttc
    51481 acgatatttt gaaagcacga ggggaaatct gatggaacgc tacgaatctc tgtttgccca
    51541 gttgaaggag cgcaaagaag gcgcattcgt tcctttcgtc acgctcggtg atccgggcat
    51601 tgagcagtca ttgaaaatta tcgatacgct aattgaagcc ggtgctgacg cgctggagtt
    51661 aggtatcccc ttctccgacc cactggcgga tggcccgacc attcaaaacg ccactctgcg
    51721 cgcctttgcg gcaggtgtga ctccggcaca atgttttgaa atgctggcac tgattcgcca
    51781 gaaacacccg accattccca ttggcctgtt gatgtatgcc aatctgg
//
LOCUS       DAJTUN010000012        50032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTUN010000012 DAJTUN010000000
VERSION     DAJTUN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.012"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..642
                     /locus_tag="OO470_000679"
     CDS             <1..642
                     /locus_tag="OO470_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6250030.1"
                     /translation="SLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLS
                     QYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNV
                     DLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIV
                     DEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            653..1744
                     /locus_tag="OO470_000680"
     CDS             653..1744
                     /locus_tag="OO470_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6250031.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1787..2647)
                     /gene="rsmI"
                     /locus_tag="OO470_000681"
     CDS             complement(1787..2647)
                     /gene="rsmI"
                     /locus_tag="OO470_000681"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6250032.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2712..4748
                     /gene="lpoA"
                     /locus_tag="OO470_000682"
     CDS             2712..4748
                     /gene="lpoA"
                     /locus_tag="OO470_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6250033.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4706..5101
                     /locus_tag="OO470_000683"
     CDS             4706..5101
                     /locus_tag="OO470_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6250034.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5121..5711
                     /gene="diaA"
                     /locus_tag="OO470_000684"
     CDS             5121..5711
                     /gene="diaA"
                     /locus_tag="OO470_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6250035.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5721..6296
                     /gene="yraP"
                     /locus_tag="OO470_000685"
     CDS             5721..6296
                     /gene="yraP"
                     /locus_tag="OO470_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6250036.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6410..7450)
                     /locus_tag="OO470_000686"
     CDS             complement(6410..7450)
                     /locus_tag="OO470_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6250037.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7523..8158)
                     /locus_tag="OO470_000687"
     CDS             complement(7523..8158)
                     /locus_tag="OO470_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6250038.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8286..8804
                     /gene="yhbO"
                     /locus_tag="OO470_000688"
     CDS             8286..8804
                     /gene="yhbO"
                     /locus_tag="OO470_000688"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6250039.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8784..9227)
                     /locus_tag="OO470_000689"
     CDS             complement(8784..9227)
                     /locus_tag="OO470_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6250040.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9278..9580
                     /gene="yhbQ"
                     /locus_tag="OO470_000690"
     CDS             9278..9580
                     /gene="yhbQ"
                     /locus_tag="OO470_000690"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6250041.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9567..10070)
                     /locus_tag="OO470_000691"
     CDS             complement(9567..10070)
                     /locus_tag="OO470_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6250042.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10064..10588)
                     /locus_tag="OO470_000692"
     CDS             complement(10064..10588)
                     /locus_tag="OO470_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6250043.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10797..11792
                     /locus_tag="OO470_000693"
     CDS             10797..11792
                     /locus_tag="OO470_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6250044.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     HYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11801..12679
                     /locus_tag="OO470_000694"
     CDS             11801..12679
                     /locus_tag="OO470_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6250045.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12760..13767
                     /locus_tag="OO470_000695"
     CDS             12760..13767
                     /locus_tag="OO470_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6250046.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13885..15129)
                     /gene="mtr"
                     /locus_tag="OO470_000696"
     CDS             complement(13885..15129)
                     /gene="mtr"
                     /locus_tag="OO470_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6250047.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15283..17172)
                     /gene="deaD"
                     /locus_tag="OO470_000697"
     CDS             complement(15283..17172)
                     /gene="deaD"
                     /locus_tag="OO470_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6250048.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRHR
                     FGGDA"
     gene            complement(17165..17245)
                     /gene="yrbN"
                     /locus_tag="OO470_000698"
     CDS             complement(17165..17245)
                     /gene="yrbN"
                     /locus_tag="OO470_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6250049.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17352..18236)
                     /gene="nlpI"
                     /locus_tag="OO470_000699"
     CDS             complement(17352..18236)
                     /gene="nlpI"
                     /locus_tag="OO470_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6250050.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18345..20480)
                     /gene="pnp"
                     /locus_tag="OO470_000700"
     CDS             complement(18345..20480)
                     /gene="pnp"
                     /locus_tag="OO470_000700"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6250051.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20727..20996)
                     /gene="rpsO"
                     /locus_tag="OO470_000701"
     CDS             complement(20727..20996)
                     /gene="rpsO"
                     /locus_tag="OO470_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6250052.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21145..22089)
                     /gene="truB"
                     /locus_tag="OO470_000702"
     CDS             complement(21145..22089)
                     /gene="truB"
                     /locus_tag="OO470_000702"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6250053.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22089..22490)
                     /gene="rbfA"
                     /locus_tag="OO470_000703"
     CDS             complement(22089..22490)
                     /gene="rbfA"
                     /locus_tag="OO470_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6250054.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22654..25302)
                     /gene="infB"
                     /locus_tag="OO470_000704"
     CDS             complement(22654..25302)
                     /gene="infB"
                     /locus_tag="OO470_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6250055.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25327..26814)
                     /gene="nusA"
                     /locus_tag="OO470_000705"
     CDS             complement(25327..26814)
                     /gene="nusA"
                     /locus_tag="OO470_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6250056.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26842..27294)
                     /gene="rimP"
                     /locus_tag="OO470_000706"
     CDS             complement(26842..27294)
                     /gene="rimP"
                     /locus_tag="OO470_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6250057.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27501..27577)
                     /locus_tag="OO470_000707"
     tRNA            complement(27501..27577)
                     /locus_tag="OO470_000707"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27541..27543),aa:Met,seq:cat)
     gene            27925..29268
                     /gene="argG"
                     /locus_tag="OO470_000708"
     CDS             27925..29268
                     /gene="argG"
                     /locus_tag="OO470_000708"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6250058.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29276..30883)
                     /locus_tag="OO470_000709"
                     /pseudo
     CDS             complement(29276..30883)
                     /locus_tag="OO470_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31361..31447)
                     /locus_tag="OO470_000710"
     tRNA            complement(31361..31447)
                     /locus_tag="OO470_000710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31411..31413),aa:Leu,seq:gag)
     gene            complement(31462..31794)
                     /gene="secG"
                     /locus_tag="OO470_000711"
     CDS             complement(31462..31794)
                     /gene="secG"
                     /locus_tag="OO470_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6250059.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32022..33359)
                     /gene="glmM"
                     /locus_tag="OO470_000712"
     CDS             complement(32022..33359)
                     /gene="glmM"
                     /locus_tag="OO470_000712"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6250060.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33352..34200)
                     /gene="folP"
                     /locus_tag="OO470_000713"
     CDS             complement(33352..34200)
                     /gene="folP"
                     /locus_tag="OO470_000713"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6250061.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34290..36224)
                     /gene="ftsH"
                     /locus_tag="OO470_000714"
     CDS             complement(34290..36224)
                     /gene="ftsH"
                     /locus_tag="OO470_000714"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6250062.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36324..36953)
                     /gene="rlmE"
                     /locus_tag="OO470_000715"
     CDS             complement(36324..36953)
                     /gene="rlmE"
                     /locus_tag="OO470_000715"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6250063.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37079..37372
                     /gene="yhbY"
                     /locus_tag="OO470_000716"
     CDS             37079..37372
                     /gene="yhbY"
                     /locus_tag="OO470_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6250064.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37528..38004)
                     /gene="greA"
                     /locus_tag="OO470_000717"
     CDS             complement(37528..38004)
                     /gene="greA"
                     /locus_tag="OO470_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6250065.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38252..39685
                     /gene="dacB"
                     /locus_tag="OO470_000718"
     CDS             38252..39685
                     /gene="dacB"
                     /locus_tag="OO470_000718"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6250066.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39871..41043)
                     /gene="cgtA"
                     /locus_tag="OO470_000719"
     CDS             complement(39871..41043)
                     /gene="cgtA"
                     /locus_tag="OO470_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6250067.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41059..42024)
                     /locus_tag="OO470_000720"
     CDS             complement(41059..42024)
                     /locus_tag="OO470_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6250068.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42151..42408)
                     /gene="rpmA"
                     /locus_tag="OO470_000721"
     CDS             complement(42151..42408)
                     /gene="rpmA"
                     /locus_tag="OO470_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6250069.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42429..42740)
                     /gene="rplU"
                     /locus_tag="OO470_000722"
     CDS             complement(42429..42740)
                     /gene="rplU"
                     /locus_tag="OO470_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6250070.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42998..43969
                     /gene="ispB"
                     /locus_tag="OO470_000723"
     CDS             42998..43969
                     /gene="ispB"
                     /locus_tag="OO470_000723"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6250071.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44196..44474
                     /gene="sfsB"
                     /locus_tag="OO470_000724"
     CDS             44196..44474
                     /gene="sfsB"
                     /locus_tag="OO470_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6250072.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44522..45781)
                     /gene="murA"
                     /locus_tag="OO470_000725"
     CDS             complement(44522..45781)
                     /gene="murA"
                     /locus_tag="OO470_000725"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6250073.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45836..46090)
                     /gene="ibaG"
                     /locus_tag="OO470_000726"
     CDS             complement(45836..46090)
                     /gene="ibaG"
                     /locus_tag="OO470_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6250074.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46250..46543)
                     /gene="mlaB"
                     /locus_tag="OO470_000727"
     CDS             complement(46250..46543)
                     /gene="mlaB"
                     /locus_tag="OO470_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6250075.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46543..47178)
                     /gene="mlaC"
                     /locus_tag="OO470_000728"
     CDS             complement(46543..47178)
                     /gene="mlaC"
                     /locus_tag="OO470_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6250076.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47197..47748)
                     /gene="mlaD"
                     /locus_tag="OO470_000729"
     CDS             complement(47197..47748)
                     /gene="mlaD"
                     /locus_tag="OO470_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6250077.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47753..48535)
                     /gene="mlaE"
                     /locus_tag="OO470_000730"
     CDS             complement(47753..48535)
                     /gene="mlaE"
                     /locus_tag="OO470_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6250078.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48543..49352)
                     /gene="mlaF"
                     /locus_tag="OO470_000731"
     CDS             complement(48543..49352)
                     /gene="mlaF"
                     /locus_tag="OO470_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6250079.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49562..>50032
                     /locus_tag="OO470_000732"
     CDS             49562..>50032
                     /locus_tag="OO470_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="YrbG; inner membrane protein involved in cell
                     envelope integrity; putative sodium ion/calcium ion
                     exchanger; in E. coli it is non essential for cell
                     viability; member of the YRBG family of cation/Ca2+
                     exchangers; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6250080.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTRE"
BASE COUNT        12235 a        13705 c        12199 g        11893 t
ORIGIN      
        1 agcctgaacg ccagttaccg ttcaccttat ggaaccttca gtgccggata cagttacggt
       61 aatgacagca gccaatacaa ttacggcgtt accggcggcg tggttatcca tcctcatggc
      121 gtgacgctct cgcaatatct gggcaacgct tttgcgctta tcgatgctaa cggggcttct
      181 ggcgtgagga tacaaaacta tccggggatt gctactgatc cctttggcta tgcagtggtt
      241 ccttatctca cgacttatca ggaaaaccgt ctctcggtag atactacgca gctgcccgat
      301 aacgtcgatc ttgaacaaac aacacagttt gtggtgccca acagaggtgc aatggtagcg
      361 gcgcgtttca acgccaatat cggttatcgc gtacttgtta cagtcagcga tcgcaacggt
      421 aaaccgttgc cctttggcgc tcttgccagc aacgatgata cggggcaaca aagtatcgtc
      481 gatgagggcg gcatactata tctctctggg atatcgagta aatcacaaag ctggactgta
      541 cgctggggaa atcaggcaga tcaacaatgt cagtttgctt ttagtacacc ggattcagaa
      601 ccaacaacct ctgtattaca aggcacagcg cagtgccatt aaggataaaa aaatgaaaag
      661 agcgcctctt ataacaggac ttttgttgat atccacatcc tgcgcttatg cctcctcagg
      721 agggtgtgga gccgacagca ctagcggtgc gacaaattac agcagtgtgg ttgatgatgt
      781 tacggtgaac cagacagata acgtgacagg acgggagttt acctctgcaa cgctaagtag
      841 cactaactgg caatacgcct gttcctgctc tgcgggtaag gcagttaaac ttgtctatat
      901 ggtcagcccc gtacttacca ccactggaca tcagacagga tattacaaac tcaatgacag
      961 cctggatatt aaaaccacat tacaggcaaa cgacattcca ggactcacaa ccgaccaggt
     1021 tgtctctgtt aacacccgat tcacacagat aaaaagcagc accgtatatt ctgctgcaac
     1081 ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga cccgttaata ttggtgcaaa
     1141 taccaccttt accctgtatg tcaccaagcc atttctcggc tcgatgacca ttccgaaaac
     1201 ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg ggaagcccgt ctacaggtga
     1261 cttccatgat ttagtcaagt tatcgattca gggaaatctc accgccccac agtcgtgcaa
     1321 aattaatcag ggcgatgtta ttaaagttaa ttttggattc atcaatggtc agaagtttac
     1381 cacccgcaat gccatgccag acggttttac tccagtagac tttgatatca cttatgactg
     1441 tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc atcgacggta caactggggt
     1501 agtagaccag tacaacctgg tcgccaggcg aagaagttca gacaatgtgc ccgatgtcgg
     1561 tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt ccttttcaga acggtatcct
     1621 tcccgttgat ccttccgggc atggcaccgt caatatgcgc gcctggccag ttaatctggt
     1681 cggtggtgag ctggaaacag gaaaatttca gggcacagcc gccattaccg tcatggtgcg
     1741 gtaaaagaag acgttacgtt ttgattaacg caacgcttcg gcactgttac ccctgctgct
     1801 ccagcgcata cttatacagc gcatttttct tcacgccatg aatttctgcg gccagcgccg
     1861 ccgctttttt cagcggcagt tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg
     1921 gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag caccatttcg cctttgcgac
     1981 ggttttcatc ttcctttacc cacgccagca gctcgccaac gggcgcgccg tgaatggttt
     2041 cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa
     2101 cgatatcttc caggctatct aacagacggt gggtagattc ataaaaaatc agcgtgcgcg
     2161 gctccgcttc aatggctttt agcgcatcac ggcggccttt tgatttggca ggtaaaaagc
     2221 cttcgtaaca gaaacggtca gatggtaaac ccgcggcgct taacgcagtg atagcagcac
     2281 acggtccggg tagcggcacc acacgaatcc ccgcttcacg gcaggtacgc accagatggt
     2341 agccaggatc gttaattagc ggcgttccgg catcggaaac cagcgcaatg ttttgcccct
     2401 cttgcagctt tgccagcagc gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa
     2461 acaaccgggc attaatccca aaatgttgca gcaataaacc ggtgtgacga gtatcctcgg
     2521 cggcaatcag atcaacggcc tgtaatacct ctaacgtacg ctgggtgata tccgccagat
     2581 tgccgattgg cgtcggtaca atgtaaagct ggccctgaga attatccgcc gattggtgtt
     2641 gtttcattgt gtcgtccgta ttgccgattt aatattgagc attgcgtaaa aaaaatatca
     2701 ctggatacat tatggtaccc tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg
     2761 ttgttctggc agccctgatt ttcgccggtt gtggcaccca tactcccgat cagtccactg
     2821 cttatatgca gggcacggcg caggctgatt ctgcctttta tcttcagcag atgcagcaaa
     2881 gctctgatga taccaggatc aactggcaat tactcgccat tcgtgcactg gtgaaagaag
     2941 gtaaaaccgg gcaggcggtt gagttgttta accaactacc gcaagaactg aacgattctc
     3001 agcgtcgcga gaaaacactg ctggcggcag agattaaact ggcgcagaaa gattttgctg
     3061 gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt agaacaaaac cagcaagcgc
     3121 gttactggca ggcaaaaatc gatgccagcc aggggcgtcc ttccattgat ttactgcgcg
     3181 cgttaattgc tcaggaaccg ctgcttggcg cgaaagaaaa acagcagaat attgatgcca
     3241 cctggcaggc gctctcctcc atgactcagg aacaggcgaa tacgctggtg atcaacgccg
     3301 acgaaaatat tctgcaaggc tggctggatc tgcagcgcgt ctggtttgat aaccgtaacg
     3361 atcccgacat gatgaaagcc gggatcgccg actggcagaa acgttatccg aacaatccgg
     3421 gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc gtttaaacca gcctcgacca
     3481 acaaaatcgc cctgctgttg ccgctgaatg gccaggcagc ggtatttggt cgcactattc
     3541 agcaaggctt tgaagccgcg aaaaatatcg gcactcagcc agtggcggct caggtagctg
     3601 ccgcacctgc cgcagacgta gcagaacaac ctcagccgca aactgcggat agcgttgcca
     3661 gcccggcaca agcctcggtt agcgatctga ccggtgatca gcctgcggcc cagccggtgc
     3721 ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc acccgcaaat ccatccgcag
     3781 agctgaaaat ctacgatacc tcatcacaac cacttagcca gatcttaagc caggttcagc
     3841 aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa taacgttgaa gagttgctga
     3901 agagcaacac cccgctgaac gtactggcac tgaaccagcc ggagaatatc gaaaaccgcg
     3961 tcaatatttg ttacttcgcg ctttcaccgg aagacgaagc gcgcgatgca gcgcgtcata
     4021 ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc acgcagtgca ttgggcgatc
     4081 gcgtagccaa tgcgtttgcg caagagtggc agaaactggg cggcggcacc gttctgcaac
     4141 aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa cggcggttct ggtattgctt
     4201 taacgggtag cccgattact cccagagcga caaccgactc cggcatgacg accaacaatc
     4261 caacgctgca aaccacgcca accgatgacc agttcaccaa taatggcggt cgtgtcgatg
     4321 cggtctacat tgtggcaacg ccgggtgaaa tcgcttttat caaaccgatg atcgccatgc
     4381 gtaacggtag ccagagcggt gcaacgctgt acgccagttc ccgcagcgca caaggcaccg
     4441 caggcccgga tttccgactg gagatggaag gcttgcagta cagcgaaatc ccgatgctgg
     4501 caggcggtaa tctgccgtta atgcagcagg cgctcagcgc ggtgaacaac gattattcgc
     4561 tggctcgcat gtatgcgatg ggcgttgatg cctggtcgtt ggcaaatcat ttctcacaaa
     4621 tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg aagtctgacg gctaacccgg
     4681 attgcgtgat taacaggaag ttatcatggc tacagtacca acaaggtcag gtagtccccg
     4741 ccagttaacc accaaacaga ccggcgatgc gtgggaagca caagcgcgtc gctggctgga
     4801 aggcaaagga ctgcggttta tcgccgctaa cgtgaacgag cgtggcggcg agatcgatct
     4861 gataatgcgt gaaggcctga ccaccgtttt tgtcgaggta cgctatcgcc gctctgcgct
     4921 ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa cacaaattat tacagactgc
     4981 ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact gtggattgcc ggttcgatgt
     5041 ggtagccttc accgggaatg aggttgagtg gattaaggat gcctttaatg accactcata
     5101 attaaggttt aaggattagc gtgcaagaaa gaattaaagc ttgcttcact gaaagcattc
     5161 aaactcaaat tgcggcggca gaggcgcttc cggatgccat ctcccgtgca gccatgacgc
     5221 tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg tggtaatgga acttccgctg
     5281 ccaatgcaca gcattttgct gccagcatga tcaaccgttt cgaaacggag cggcccagct
     5341 tacctgccat tgcactaaat actgataatg ttgtcttaac ggcgattgcc aacgatcgct
     5401 tacatgatga agtgtatgca aaacaggtgc gggcgctggg tcatgcggga gatgtattgt
     5461 tagccatttc cacccgtggc aacagccgcg atattgttaa agcagttgaa gccgccgtta
     5521 cgcgtgatat gaccattgtg gcattgaccg gctatgacgg cggcgaactt gcaggtttgt
     5581 tagggccaca ggatgtggag atccgcattc cttcgcatcg tagtgctcgc attcaggaaa
     5641 tgcatatgct gacggtaaat tgcctgtgcg atctgatcga taacacgctt ttccctcacc
     5701 aggatgatta aggagaatac atgaaggcat tatcgccaat cgcagtcctt atttccgcgc
     5761 tgctgttgca aggttgtgtt gccgctgccg tagtgggtac cgctgctgtg ggtaccaaag
     5821 ccgcaactga cccacgcagt gtcggcaccc aggtggacga tggtaccctg gaagtgcgcg
     5881 tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga aacccgtatt aatgtcacgg
     5941 cctatcaggg caaagtgctg ctggttgggc agtcaccaaa tgctgaactt tcggctcgcg
     6001 ccaaacagat tgctatgggc gtagacggtg ccaacgaagt gtataacgag attcgtcagg
     6061 gccagccgat tggtctgggc gaagcatcta acgatacgtg gatcaccacc aaagtgcgtt
     6121 cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt gaaagtgacc accgaaaacg
     6181 gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc gaaagcggcg gcagatattg
     6241 ccagccgggt gagcggcgta aagcgggtta ccacagcgtt tacgtttatt aaatagcagc
     6301 cccttgtaat gcctgatgcg acgcttgccg cgtcttatca ggcctacaac gatgcaaacc
     6361 gtaggtcgga taaggcgttt acgccgcatc cgacatgttt tccctcaaat cagaacaaca
     6421 gcgccagccc gccgacaatc acaccagaca ctgccaccat cgcccctgtc agccataagg
     6481 ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg caaactcact gccgggagcg
     6541 tcatcaacaa tgccagcgcc ggagcggttc ccatacctgc cagcatcatc gtttgtacaa
     6601 tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc tgctaccgcc atcgccacca
     6661 cccacatcag gctgttatcg acagcaccat cggcatgggg gaataaccag acgcgtgcgg
     6721 cacccaacac cagtactgca aggatgcaaa ccgggatcgt actccagaaa agtgtccata
     6781 gcgccctgcc ccagcggcta aaaaaaccgc tctgtgcttc cggtatgtca atttcgaccg
     6841 gtgcctgcgt ttgcagtgtt tcacgcaccc atttttgcac cagcgtcgca atcagcaacg
     6901 ccatcaccag cccggccacc agacgaatcg ccgcaaaacc ccagccgagg acaaacccca
     6961 taaacaccag cgtcgccggg tttaacaccg gattgcccat ccagaatgcc agcgcaccgc
     7021 ccatcgatac ctgttggcga cgcattcccg ccgcgaccgg agccgcacag caggtacaca
     7081 tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc gcgaaagcgc gattgcccaa
     7141 gcgtacgcaa caaccagtca cgcgggatca gcacctgaat caacgaaccg aggatcaccc
     7201 ccagcaccgc cgctttccat actgcgagga aatagatcat cgcgtaatcc aacgccgctt
     7261 gccacggatt agcatccgcc tgcgcaagga tagatttacc gatactgtgg gtttcggctg
     7321 cagtaaacgc tttgccgtag taaggttgcc atttcacata ccagagaccg gcaatgacaa
     7381 cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt tgccgcctga gatgaagact
     7441 gaccagtcat agcattcccc aggaataagt tatgataatt gagcgcgtga atattacgct
     7501 cactatcaat tcttgggaat aattattcag ctcttttgcg taattctgaa gagcttaaaa
     7561 tcgtcacgcc ttcatgctca ggccgcatcg cctctgccag catgacccgc gccacgtctc
     7621 tcgcatcaat ggatttccag ttacctggta acaaacggaa cagcggcgca aaaagcgttt
     7681 cgttcatccg ctgtttgcta cgatcgccca gtaacatcga cgggcgagca atggtcaatt
     7741 tcggccagtt ctgggcaatt aatgcttctt ccatctcccc tttgacgcgg ttatagaaaa
     7801 acggcgagtg ggcattggca cccatagcac tgaccaccag catatgctgc gcgcccagtc
     7861 gccgcccggt taatgcggta tccactacca gcgtgtaatc ggcatgaata aacgcctctt
     7921 tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa cacaatgtcg atgggttcgg
     7981 tgacctgcgc caacgcgtca ctcagttgcg gatcatgggg attaaacacc ccgggcatat
     8041 cgcccaacgg acgtcgcgtc ggcgcagcaa tggcgttaac tttcggttca ttaatcaaca
     8101 tccgcagcag gtgaccgccc accagccccg ttgcgcctgt aatcagtacc tgactcatct
     8161 tcgctccttt acagaattgt ctgccttgcg ctccccggct catgcaccag tcttaataga
     8221 ccatgaggta attatccccg attgtggaaa attcgcctca tccaatgcaa caacgcggag
     8281 gaagcatgag taagaaaatt gccgttttaa tcactgatga atttgaagat tcagaattta
     8341 cttcacccgc agacgagttc cgtaaagccg gacacgaagt gattactatt gaaaaacaag
     8401 cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt gaccatcgat aaatccatcg
     8461 atgaagtgac gccagcggag tttgatgccc tgttgctacc gggtggtcat tcaccggatt
     8521 atctgcgtgg cgacaaccgt tttgtcacct ttacccgtga ttttgtgaat agcggcaaac
     8581 ctgttttcgc catctgtcac ggtccgcagc tactgatcag cgccgatgtg attcgggggc
     8641 gcaaactgac cgcagttaaa ccgatcatta ttgatgttaa aaatgcgggc gtggaatttt
     8701 acgatcagga agtcgtggtt gataaagatc agctggttac cagccggaca ccggacgatc
     8761 tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc ctgagtcgcg tagccaaatc
     8821 atttttttac caaagcccag cgtgttgtcg gtgaatttga tttcatcgag ccggatttcc
     8881 cacaccgggg ccgacagcat tctggcaacc ggaaagcgac gattgtacgc cttgcgcgcg
     8941 aggtcgcttt cctcaccttc cagcctgcgg atctcacctt taaactgcac accgcgaatt
     9001 aacgctaccg ttttcggctg accgtttacc gttccggcaa ccgcagcctg cggcccactc
     9061 atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga aggcgacttt ctgcgcatca
     9121 aaaagataaa aggcattagc gcaccagagt tccccttcct gctgcacaca ccaggtgaca
     9181 acatgttgtt ttgccagcca acggctgatg gcgatgagtg tttccattgc tgttctcttt
     9241 tatactgtgg gcctgacctt aacatgcgat gcagacgatg acaccctggt ttctttactt
     9301 gatccgtacc gccgacaata agctttatac cgggatcacc acggatgtcg aacgccgcta
     9361 tcagcagcac caaagcggca aaggggcgaa agcactgcgc gggaaaggag aactgacgct
     9421 ggcgttttct gcgccagtgg gcgaccgttc gctggcacta cgggcggaat atcgcgttaa
     9481 gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa ggcgcagggt ttgcagaact
     9541 gttgagcagc ctgcaaaccc cggagattaa aagcgattga aatgctcgtg atactcaacc
     9601 agaccggtta cgccattcag cgcgtcatcc gctaaacgat gtaccaggaa ggcgctttca
     9661 gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca gttcaaagcc gaaacgactg
     9721 tacagcgccg gatcgcccag cgtcaccact gcggcatagc cgaactcatt aagcgaatcg
     9781 agtccttcat agaccagttg gcgtgccagc ccctgtccac ggtatttttc atcaaccgcc
     9841 aacggtgcca tgccgaccca ttgcaggtct tcgccctgca catcaaccgg actaaatgcc
     9901 acatagccaa tgacctgacc ttcgtcatct gtcgccacca gccccagcgt cagaaagcca
     9961 tcttcacgca gatcgtgaac cagcttcgct tccgcatcac tttcgaatga gcgacgcagc
    10021 agggcatcaa tacccggcgc atcaatggga atttctactc gaattagcat ggttcaccta
    10081 ccgatgtctg tttggtttca ggcgcggttt tcattcccgc ctcaacaaaa tccgccagtt
    10141 gcagcagcat catgcgtaag gctttcggca tttgctccag ttcaatggcg tccatcaggt
    10201 ttttcacata cacccccagc tccgtatcgc cttcaatcac cagccgacgc tggaagaaga
    10261 gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag atcgctggcg tcggcactaa
    10321 aactcacatc agcttgcgtg ttctggctga ccaccagttt gccattcacc accgaggtaa
    10381 accattgcag gtcaatatca cgcacatgaa tacttaacca gcggccttca agaaactcca
    10441 gctcgccatc atccagcgcc tgacggaatt gccagcttaa gacctgctca agaacctggc
    10501 gttttagcgc aaatggcgtc agttttaccg gtacactcaa cagagatggc cccaaatgca
    10561 caatacggga acgcagttta tccaacacga gttttactcc ctgtttcaac aatcatccta
    10621 ttttgccata tcagaaaaat aacatagcgg tataaatcaa caattccata tgaaattgct
    10681 gctaccacca atacaacttt aactgcctta aatcaaaaat tgtcgcagca aggttaacta
    10741 aaatcccagt tcgttaacat ttttgcgttt tgatagcgca accttcagga aaaattatgg
    10801 agctgctctg ccctgccgga aatctcccgg cgcttaaggc ggccatcgaa aacggcgcag
    10861 atgctgttta tatcgggcta aaagatgata ccaatgcccg tcacttcgcc ggccttaact
    10921 ttaccgagaa aaaattgcag gaagcggtga gttttgtcca tcaacatcgc cgcaaacttc
    10981 acatcgcgat taacactttt gcgcatccgg acggttacgc ccgttggcag cgtgccgtgg
    11041 atatggcggc gcagctgggt gccgacgcgc tgatcctcgc cgacctcgcc atgctggagt
    11101 acgccgccga gcgttacccg catattgaac gtcatgtgtc agtgcaggct tcggcgacca
    11161 atgaagaggc gattaacttt tatcatcgcc attttgacgt tgcccgcgtg gtgctgccgc
    11221 gcgtgttgtc gattcatcag gtgaaacagc tggcacgggt tactcctgtc ccgctggaag
    11281 tgtttgcttt cggcagcctg tgcattatgt cggaaggccg ttgctatctg tcgtcgtatc
    11341 tgacgggtga gtcgcccaac actgtgggtg cgtgttctcc ggcccgtttc gtgcgctggc
    11401 aacaaacgcc gcaggggctg gaatcccgcc tgaacgaagt gctgatcgac cattatcagg
    11461 acggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg ttatctggtg gacggcgagc
    11521 gctatcacgc gctggaagaa ccaaccagtc tcaataccct ggaactgctg ccggagttaa
    11581 tggctgcgaa tattgcttcg gtgaaaattg aaggccgcca gcgtagcccg gcgtatgtca
    11641 gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg taaggccgat ccgcaaaact
    11701 tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat gtccgaaggc acgcaaacca
    11761 cccttggcgc gtatcaccgt aaatggcagt gagaagagca atgaaatatt ccttagggcc
    11821 agtgctgtgg tactggccaa aagagacgct ggaagatttt tatcagcaag ccgccgccag
    11881 cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc aagcgccggg caaccaaagt
    11941 tggcgactgt ctggagatgg caaaatcgct cgccggaagt ggcaagcaga ttgtgctctc
    12001 cacgctggcg ctggtgcagg catcctctga actgggcgaa ctgaaacgct atgttgagaa
    12061 cggtgaattt ctgattgaag ccagcgatct cggcgtggtg aatatgtgcg ctgaacgcaa
    12121 actgccgttc gtcgccgggc acgcgctgaa ctgctacaac gcggtgacgc tgaaaatatt
    12181 gctcaaacag ggcatgatgc gctggtgtat gccggtggag ctttcccgcg actggctggt
    12241 gaatctgctt aatcagtgcg atgagctggg cattcgcaac cagtttgaag tggaagtcct
    12301 gagctacggt catctaccgc tggcctactc cgcccgctgc tttaccgcgc gttcggaaga
    12361 ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat ccgaacgggc gcaacgtgct
    12421 gtcgcaggaa aaccaacaag tgtttgtact caatggcatt cagaccatga gcggctacgt
    12481 ttacaacctc ggtaacgagc tggcatccat gcagggattg gttgatgtag tccgcctgtc
    12541 accgcagggc actgacactt tcgcgatgct cgacgccttc cgcgctaatg aaaatggcgc
    12601 tgtgccactg ccgttgacgg caaacagcga ctgtaacggc tactggcggc ggctggcagg
    12661 actggagctg caagcctaag taaatagctc actttgttaa caactttaac tattctttaa
    12721 tgcagtatta aagattaatc ggtaacaaag tgagctgtta tgactgataa aaccattgcg
    12781 ttttcgctac tcgatctggc ccccataccc gaaggctctt cagcgcggga agcgttctcc
    12841 cactctctcg atctcgcccg tctggctgaa aagcgcggct atcatcgcta ctggctggca
    12901 gaacaccaca atatgaccgg cattgccagt gctgcaacat cggtattgat tggctatctg
    12961 gcggcgaata ccaccacgct gcatctgggg tctggcggcg tgatgttgcc taaccactta
    13021 ccgttggtca ttgccgaaca gttcggcaca ctcaatacgc tctatccggg gcgaatcgat
    13081 ttggggctgg gtcgtgctcc gggtagtgac caacggacaa tgatggcgct acgtcgtcat
    13141 atgagcggcg atattgataa tttcccccgc gatgtcgcgg agctggtgga ctggtttgac
    13201 gcccgcgatc ccaatccgca tgtgcgcccg gtaccaggct atggcgagaa aatccccgtg
    13261 tggttgttag gctccagcct ttacagtgca caactggcgg cgcagcttgg tctgccattt
    13321 gcgtttgcct cacacttcgc gccggatatg ttgttccagg cgctgcatct ttatcgcagt
    13381 aacttcaaac cgtcggctcg gctggaaaaa ccatacgcga tggtgtgcat caatattatc
    13441 gccgccgaca gcaaccgcga cgctgaattt ctgtttacct caatgcagca agcctttgtg
    13501 aagctgcgcc gtggcgaaac cgggcaactg ccgccgccga ttcaaaatat ggatcagttc
    13561 tggtcaccgt ctgagcagta tgacgtgcag caggcgctga gtatgtcgtt ggtaggcgat
    13621 aaagcgaaag tgcgtcatgg tttgcagtcg atcctgcgcg aaaccgacgc tgatgagatt
    13681 atggttaacg ggcagatttt cgaccaccag gcgcgcctgc attcgtttga gctggcgatg
    13741 gatgttaagg aagagttgtt gggatagtgt gtcttaacgc gggaagcctt atccgagctg
    13801 gcaacgctgt cctacgtagg cctgataagc gaagcgcatc aggcattgtg taggcagcag
    13861 aaatgtcgga taaggcaccg ctgattactg atacaccggc agtaaattaa agctcgataa
    13921 aatatgcacc agtgcgttgc cgacgccaaa caccagaatc agcgcaatca tcggcttgcc
    13981 accccagacg cggaatttcg ggctgccaaa gcgtttacgc gatgcgcggg ccagcagtgc
    14041 cggaacaatt gccgcccaga tggtagccgc taaaccagca taaccaatgg catacaggaa
    14101 tccgttcggg aacagcagac cacccacaac aggcggggca aaggtcagca atgccgtttt
    14161 caagcggccc acagccgagt cgtcgaaacc aaacagatct gccagatagt caaacaaacc
    14221 cagcgttacg ccaaggaacg aactcgctac cgcaaagttt gagaacacga ccagcagaag
    14281 gtccaggcta cggctgttca gcacgccgct taatgcttgt accaacacat caatattacc
    14341 gcccttctcg gcgatgccga taaactccgg acgagggatg ttgcccattg tcgccagcaa
    14401 ccagatggta tacagcgcca gtgccatcag cgtaccgtac accaggcatt tcacgatggt
    14461 tttcggatct ttgccgtaat acttcatcag gcttggcacg ttaccgtgat aaccaaacga
    14521 cgccaggcag aacggcaggg tcatcagcaa atacggcgca taagatgcat tgctttcggc
    14581 aacgttgaac aatgtcgcag gctgcacatg ccccagcagg ctaccgaagg tgaggaagaa
    14641 ggtaatgact ttcgccccca gcacaatcgc cgtcatgcgg ctgacggctt tagtactcaa
    14701 ccacaccaca aacgctacca gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt
    14761 tagcgacatc tctgcgaagg tgtgatgcag aatcgaacca ctggcagaaa tataggcata
    14821 ggtcaggata tagagcacaa aggcaatgga aatgccgttg accacgttcc agcctttgcc
    14881 cagcaaatct ttggtgatgg tgtcaaaact cgaaccgatt cggtaattca ggttagcttc
    14941 cagaatcatc aagccggaat gcagcataca gaaccaggta aagatcagcg ctgccattga
    15001 ccagaaaaac cacgccccgg acatgaccac tggcagagaa aacatccctg cgccaataat
    15061 ggtgccgccg ataatcacca cgccgccaag cagcgacggt gacgtttggg tggtggttcg
    15121 tgttgccatg agggcttctc tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca
    15181 ctgtaccagt acatgagtac aaaagacaga aaaaaagccc cgatggttaa aatcggggct
    15241 gtatatatta ttttacagat tgtgttcgct gttcagcgat gattacgcat caccaccgaa
    15301 acgatgacga ccggtagagt catcacgacg cggtgcgcgg ccttcacgac gttcgccgct
    15361 aaaacgacga ccatcaccac gaccaccttc acggcgttca ccgctgaagt tacgaccgcc
    15421 ttcacgacgc tcgccaccga aaccacgacc accgccacga cgctcaccgc cagtatgcgg
    15481 ctgtgcatcg cccagtaact gcatgttcat cggcttgttg agaatgcgag tgcgcgtaaa
    15541 gtgttgcagc acttcacccg gcataccttt cggcagttcg atggtggagt gagaagcaaa
    15601 cagcttgatg ttaccgatgt aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac
    15661 gatatgacga acttcaacac catcatcgcg gcccacttca atgcggtaca gctgcatatc
    15721 gccaacatca cgacgttcac gacgcggacg atcttcacgg tcgccacgcg ggccacggtc
    15781 gttacgatcg cgcggaccac ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg
    15841 cgcatctggc ggaacaatca gagtacgttc accctgtgcc attttcagca gtgccgcagc
    15901 cagagtttcg agatccagct cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc
    15961 gcggtattga tccagatcgc tgctttccag ctgctgctgt actttagcgg cgaatttttc
    16021 cagacggcgt ttgcccagca gttctgcgtt cggcagttct acttccggaa tagtcagctt
    16081 catagtacgt tcaatgttgc gcagcagacg acgctcgcgg ttctcaacga acagcagcgc
    16141 gcggccagca cgacccgcac gaccggtacg accgatacgg tgaacgtaag actcagaatc
    16201 catcgggata tcgtagttaa ccaccaggct gatacgctca acgtccaggc cacgggccgc
    16261 aacgtcggtc gcaatcagga tgtccagacg accgtcttta aggcgttcca gtgtctgctc
    16321 acgcagcgcc tggttcatgt caccgttcag cgcggcgcta ttgtagccgt tacgctcaag
    16381 agcttcagcc acttccagag tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa
    16441 atcttccgct tccaggaaac gtaccagtgc ttcgttttta cgcatacccc acacagtcca
    16501 gtagctctgg ctgatgtcag gacgggtagt cacgctggac tgaatacgca cttcctgcgg
    16561 ctctttcata aagcggcggg taatgcgacg aatcgcttcc ggcatggttg cagagaacag
    16621 agcggtctga tgaccttccg ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc
    16681 catgcgcagc atttcgtcag cttcatccag aaccagaccg ctcagtttag agaggtccag
    16741 agtgccacgt ttcaggtggt ccagcagacg gcccggagta ccgacaacga tctgcggccc
    16801 ctgacgcagg gcgcgtaatt gcacgtcata acgctggccg ccgtacaggg caaccacatt
    16861 tacgccgcgc atgtgtttag agaaatccgt cattgcttca gcaacctgta ccgccagttc
    16921 gcgggtcggt gccagcacca gaatctgtgg tgctttcagc tcaggatcaa gattctgcag
    16981 cagaggtaaa gagaatgctg cagtttttcc gctccccgtc tgggccatac ccagaacgtc
    17041 gcggccattc agcagatgtg gaatacactc tgcctgaatt ggagatggtt tttcgtaacc
    17101 cagatcgtta agggcttcaa ggataggagc cttcaggccc agatctgcaa aagtggtttc
    17161 gaattcagcc atgtagtacg tgtgcctcaa aattaatggc ggccagtcta cataactcat
    17221 catgaaattg atcagcaatt ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta
    17281 acgaacaaca acgccctcac ccgttaaggt gatggcaatc aaaaaagatt acgggctgat
    17341 gtgtacgtca gctattgctg gtccgattct gccaggtcat cttggtcctg gcccaggagc
    17401 gataattcca acaatgcgta tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc
    17461 agtttgaaca gtgccgtggc gctgtccaaa tcccccagac ttaggtagta cttacctaaa
    17521 tagaagttgg tttcactgag atgctcagcg agcgaggtgt tatccgttgc gtccgccttg
    17581 agcctttcca ttaacgtttg ttcgctaatg ttgcccaggt agaactcgac aatgttccat
    17641 ccccactgtt ccttatccga tttttcgaag tgctgtttca atacttcttt agcctgcttc
    17701 tcatcgagct tctgctcggc gagataaagc cacagactac ggaaaggatc attgggatcg
    17761 tcttgataaa acgccagcag atcatcttgc gctaacttgt cacgaccgcc gtaatataat
    17821 gcaatcccgc gattcaagtg cgcgtagttg taagttggat caagctcaag tacagaatca
    17881 aacgcttcat aggcagcatc aaaattgcct gcctgcgtta aatatatgcc taagtaattg
    17941 aatacttcag gcatatccgg tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat
    18001 gccctcagac cgagactatc atacaacact ccgcgctcat ataaaagctg tgcgcgttcg
    18061 tcatcggtta aagcccgact ggcaaggatt tgttccatac gtgccagaat cacttcctgc
    18121 tgtaaagtcg gttgcaatgg taccgcgagg acttcacttt tacgccagga agtattactg
    18181 catcctgcaa gcgtaagtgc tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc
    18241 cactcccgaa gaccacggtt gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg
    18301 cccggttaaa agccccccgc cgcagcggag ggcaaatggc aaccttactc gccctgttca
    18361 gcagccggag cttccggtgc tgcagcaggt tgagactgct cagtcgcttc tttaatgctc
    18421 agacggatac ggccctggcg atcaacttcc agaactttca ccggtacttc ctgacccatc
    18481 tgcaggtaat cggtcacttt ctcaacgcgt ttgtcagcga tttgagagat gtgtaccaga
    18541 ccttctttac cgccgccgat ggcaacaaat gcgccaaagt caacgatacg ggtcacttta
    18601 ccagtgtaga cgcggcccac ttcgatttct gcagtgatct cttcgatacg acgaatagca
    18661 tgtttcgctt tctcgccgtc ggtcgctgca atcttcacag taccgtcatc ttcgatttcg
    18721 atggtggtgc cagtttcttc ggtcagggca cggataacag agccgccttt accgataacg
    18781 tctttgatct tgtccgggtt gatcttgatg gtatggatac gtggtgcgaa ctcagagata
    18841 tcgccacgcg gcgcgttgat cgcctgttcc attacgccca ggatatgcag acgcgcacct
    18901 ttagcctggt tcagcgcaac ctgcatgatc tctttggtga taccttcaat tttgatatcc
    18961 atctgcaatg cagagatacc ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg
    19021 tgatcttcgt cgcccaaaat gtcagacagt acaacgtagt tgtcgccttc tttcaccaga
    19081 cccattgcga tacccgcaac ggcagctttg atcggcacac ctgcgtccat cagcgccaga
    19141 gacgcgccgc acacggaagc catagaagag gaaccgttgg attcagtgat ttcagacaca
    19201 acacgtacgg tgtacgggaa tttgtccata tccggcatga ctgccagcac gccgcgcttc
    19261 gccagacgac cgtgaccaat ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct
    19321 acggagtacg gagggaagtt gtagtggaac aggaaggtat cggtacgttc gcccatcagt
    19381 tcatcaagaa cctgcgcgtc acgagcagta cccagcgttg cggtaaccag cgcctgcgtt
    19441 tcaccacggg tgaacagcgc agaaccgtga gtacgcggca gcacgccagt acgcacatcc
    19501 agaccacgga tcatatcttt ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg
    19561 ctacgaacaa cgtttttctc gatcgcgtgc agaatttcac ccagttcgtt ttcgtccagg
    19621 gtttcgtctt cagcaagcag cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca
    19681 taacgctctt gtttgtcggt gatgcggtaa gcatcgctca ggcgagcttc agccagtgca
    19741 gcaacgcgcg cgttcagcgc ttcgtttacc ggctccggct gccagtccca acgtggttta
    19801 ccggcttctt tcaccagttc attgatgttc tgaataacaa cctgctgttg ttcatgaccg
    19861 aacactactg cgcccagcat ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc
    19921 agtacagcgg cttcagtacc ggcaacaacc agatccagtt tgctctcttt caactcgtcc
    19981 tgagtcgggt tcagtacgta ctggtcattg atgtaaccta cgcgagcagc accaatcgga
    20041 ccattgaacg gaataccaga cagagacagt gctgcggaag caccaatcat cgcgacgata
    20101 tccgggttaa cttgcgggtt aacagaaacc acggtggcga taacctgaac ttcgttgacg
    20161 aagccttccg ggaacagcgg ccgaatcggg cggtcaatca gacgcgcgat cagggtttcg
    20221 ccttcgcttg ggcggccttc acgacggaag aagctacccg ggatacgacc agcagcgtag
    20281 gtacgctcct gatagttaac ggtcagtggg aagaagtcct gacctggttt ggcttttttc
    20341 tggccaacaa cggtaacaaa taccgcggtg tcatccatgc taaccataac agcggcagta
    20401 gcctgacgag ccatcatgcc ggtttccaga gtcacggtgt gttggccgta ctggaattta
    20461 cgaacgatcg gattaagcaa tgtaatatcc tttctctttc ttagacagta ccttacggca
    20521 ctggtgttaa tacccgatct tctgcgcatc ctcgcgacta atgacaaccc taacccagct
    20581 ctatgtgggt aaagcctctc attagccgcg cgaacctctg caacggaaga tcattcatag
    20641 caacaataca ttagtttcca gtgaattgct gccgtcagct tgaaaaaagg ggccactcag
    20701 gccccctttt ctgaaactcg caagaattag cgacgcagac ccaggcgctc gatgagctgg
    20761 gtgtaacgtg ctacgtcttt acgtttcagg tagtcgagca gtttacgacg ctgagaaacc
    20821 atgcgcagca gaccacgacg gctgtggtga tcttttttgt gctctgcaaa gtggccctgc
    20881 aggtggttga tctgtgcagt cagcagtgct acctgaactt cagtagaacc ggtgtcgttt
    20941 gcgtcacgac caaactcaga aacgatttta gctgttgctt cagtacttag agacatttta
    21001 aaactccaaa gtatatagaa tgaaaggacg ccgatctcta attcagcgat cccagtgtac
    21061 gttacgcaaa gtgttaaaca atttacgcga cgttaagcgg cagtattcta ctcgtagcga
    21121 cctgttatcg caagacggtt aacattacgc cgggtattca accaccaggc gacgaggcgc
    21181 aacgcggcct tcatcgtcaa tttcgcccat accgataaat ttgccgttct caccttctgt
    21241 aacgcgaacc agtccttcca gcggcgcacc ggatgtacga actgggttac catttttgaa
    21301 gtaaacagaa gacgttaacg gaagattcac caccgggtag tccgaagctg gactgtccat
    21361 tggcatcagt aatggatcaa gtaactccgc agccggaata tcctgctgtt cggcttgttc
    21421 gaccagttca cgcagatgtt ccagggtcac aatccgttca accggatatt tacttaccgc
    21481 cagacgacgc aggtaaataa catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat
    21541 gatggtgcgg atataagtgc cttttgagca gtgaatttcc agctccagct cattgccttc
    21601 gtggcgaata aacagcaatt cataaacggt aatcgggcgc gcttcacgcg gtacttcaat
    21661 gccctgacgc gcatattcgt acagtttttt accctgatat ttcagtgctg aatacatcga
    21721 agggatctgt tcgatatcgc cacggaaagt atccagcgcc gccgccagtt gctctgcgct
    21781 aaaggttacc ggacgttctt caacgatttg tccgtcggca tcagaagtat cggtacgctg
    21841 tccaagacgc gcaatgaccc gatagcgttt gtcggagtcc agcagatact gggaaaactt
    21901 cgtcgcttcc ccgaggcaaa ttggcaacat gccagttgcc agcgggtcca gcgcaccggt
    21961 atgcccggca cggttggcgt tataaatccg tttcactttt tgcagcgcat cattgctgga
    22021 cataccctgc ggtttatcca gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg
    22081 acgactcatt agtcctcctt gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg
    22141 accacgctgg tcaccaggtt tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag
    22201 aaggtcagtt ccggcacgat acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa
    22261 ccagaagctt cttgcaacgc tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg
    22321 aggaacgtca catatacttt ggcatacgcc aggtcgcgag acatttcgac accggaaacg
    22381 gtggtcatca tgcccaggcg aggatcttta atttcacgct gcaggatgag agcgatctct
    22441 ttttgcattt cctgcgctac gcgctgcggg cgaccaaatt ctttcgccat aataaattct
    22501 cctgacaaaa aaggggctgt tagccccttt ttaaaattaa tttcaggtgg aagggctgtt
    22561 cacgttggcc tgataagacg cgccagcgtc acatcaggca atccatgccg gatgcggcgt
    22621 aaacgcctta tcccgcatgg aaccctaaaa accttaagcg atggtacgtt ggatctcgat
    22681 gatttcgaat acttcgatca catcgccagt gcggacgtcg ttgtagttct taacgccgat
    22741 accacattcc ataccgttac ggacttcgtt aacgtcatct ttgaagcggc gcagggactc
    22801 cagctcgcct tcgtagataa ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt
    22861 aaccacacct tcggtaacca tacagcctgc gatggcacca aatttcggcg atttgaacac
    22921 gtcacgaact tccgccagac cgataatctg ctgtttcagt tccggagaca gcataccgct
    22981 catcgccgct ttcacttcgt caatcaggtt atagatgacg gagtagtaac gcagatccag
    23041 gctttccgct tcaatcactt tacgtgcaga ggcatcagca cgtacgttga agccaaccag
    23101 gatggcgttg gacgccgcag ccagggtggc gtcggtttcg gtgataccac ctacgccaga
    23161 accgatgatc ttcactttaa cttcgtcagt agacagtttc agcaaggagt cggagatcgc
    23221 ttccacagaa ccctgtacgt ctgccttcag gacgatgttc acttcgtgaa cttcgccttc
    23281 ggtcatgttg gcgaacatgt tctcgagttt agatttctgc tgacgcgcca gtttaacttc
    23341 gcggaattta ccctgacgat acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac
    23401 ggtaacttca tcacccgcag ccggtacgcc ggacaggccg aggatttcca ccggaatgga
    23461 cggacccgct tccagcactt cctgacccag ttcgttacgc atcgcacgaa cacgaccgta
    23521 ttcgaagcca cacagaacga tatcgccctt gtgcagagta ccttcacgta ccagaacggt
    23581 agcaaccgga ccacgacctt tatcgaggaa ggattcgata accgcaccgc tcgccatacc
    23641 tttacgtacc gctttcagct ccagaacttc cgcctgcagc aggatagcgt ccagcagttc
    23701 atcgatacca gtacccgctt tcgcagacac atgtacgaac tggctttcac cgccccactc
    23761 ttccggcagg atgccgtact gggagagttc gttcttaacg cgatccggat cggcttctgg
    23821 tttatcgatc ttgttcactg caacgaccac cggaacctgc gccgctttcg cgtgctggat
    23881 tgcttcgatc gtctgcggca ttacgccgtc gtcggcagca acaaccagga ctacgatgtc
    23941 cgttgcctgc gcaccacgag cacgcattga agtaaacgcg gcgtgccccg gggtgtccag
    24001 gaaggtgatc atgccgtttt cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc
    24061 gcccgcttcg ccagaggcca ctttcgttga acgaatgtag tccagcagag aggttttacc
    24121 gtggtcaacg tgacccatga tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt
    24181 gtcacggtcg ctcattaccg cctcttccag ctcgttttca cgacgcagga taactttatg
    24241 gcccatctct tcagcaacca gctgtgcggt ttcctgatcg ataacctggt tgatggttgc
    24301 cattgcgccc agtttcatca tcgctttgat gacctgagag cctttaaccg ccatcttgtt
    24361 cgccagttcg ccaacggtga tagtttcgcc gatcacaacg tcacggttaa cggcctgagc
    24421 aggcttctgg aagccttgct gcagcgaaga acctttacgt tttccgcctt taccgccacg
    24481 tactgctgcg cgtgcttctt cacgatcagc ttttgattca gcgtgtttgt tgcctttctt
    24541 cggacgcgct gctttcgcgt tacgaccacg gccacggccg ccttcgactt cacgatcgct
    24601 ttcgtcttct gcctggcgag catgttgaga agtagtgacg tgataatcgc tggaatcttc
    24661 agtcggttcc gcgttatcag tccatttgtt ttcttccgcc atacgacgtg cttcttcagc
    24721 aacgcgacgt gcttcttctt cgagtttacg acgcgcttct tcttcagctt tacgcttgag
    24781 ctctgcagct tcctgctcac ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc
    24841 ttgttgattg ctcactttgt ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc
    24901 acgcttagct tgttctgcgg cctcacgttc agctttttgt tgcgcctcgc gtttagccga
    24961 ttcttctgcc tcacgacggg cttgctcttc cgctgcaagg cgttcagcct cttgcggatc
    25021 gcgtttcaca aaggtgcgtt tcttgcggac ttcgatttgt accgatttgc tttttccacc
    25081 ggtaccagga atgttaaggg tgctgcgtgt tttacgttgc agcgtcaatt tgtccgggcc
    25141 tgaatttttc tgattcaggt ggtcaatcaa agtctgtttc tcttgtgcag acacagagtc
    25201 gtcagcagac ttccggatac ctgcatcagc aaattgctgt accaggcgtt ccacggaggt
    25261 ctgtcgctct gcggccagcg ttttaatcgt tacatctgtc atgctgttcc ttcctgctac
    25321 agtttattac gcttcgtcac cgaaccagca aatattacgg gcagccataa tcagtgctcc
    25381 ggctttttcg tcggtcaacc cttcgatatc agccagatca tcaatgccct gttcggcgag
    25441 atcttccagc gtacaaacgc cacgggcggc cagtttgaat gccaaatcac gatctacccc
    25501 ttcaaggttc agcagatcgt cagccggttt gttatcaccg aggctttctt cctgggcctg
    25561 tgcaatggtg gccagtgcat ttttagcacg ctcgcgcagt gcttcaacgg tcggctcatc
    25621 aaggccttcg atttccaaca gctctttcat cggcacatag gccagttctt ccagcgtcga
    25681 gaaaccttct tctaccagaa cagtcgcgaa gtcttcgtcg atgtcgagat atttggtgaa
    25741 ggtgtcgatc gctgcgtgcg cttccgcctg atgcttagcc tgcaggtcgt caacggtcat
    25801 tacgttgagt tcccaaccgc tcagctgcga agccagacgc acgttctgac cgttacggcc
    25861 aatcgcctgc gccaggttac cagcttcaac ggcgatatcc atggtgtgtt tatcttcatc
    25921 caccacgata gaagcaacgt ctgccggtgc cattgcgtta atcacaaact gcgccgggtt
    25981 atcatcccac aggacgatat cgatacgctc gccgcccagt tcagtagaaa ccgcctgaac
    26041 acgcgcgcca cgcataccta cgcaagcacc taccggatcg atacgtttat cgttggtttt
    26101 caccgcgatt ttcgcacgag aacccgggtc gcgagccgct gctttaattt caatcacttc
    26161 ttcgccgatt tccggcactt caatacggaa cagttcgatc agcatttccg gcttggaacg
    26221 agtgacgaac agttgcgcgc cacgcgcttc cgggcgaacg gaataaagca cgccacgaac
    26281 gcggtcgcca gggcggaagt tttcacgcgg cagcatatct tcgcgcagga tcacggcttc
    26341 agcgttgttg cccagatcca gagagatgtt gtcgcggttt acttttttca ccacgccggt
    26401 gatgatttca ccttcgtgtt cacggaactg atcaaccacc atcgcacgtt cggcttcacg
    26461 cactttctgc acgataacct gttttgccgt ctgggtggtg atacggtcaa aggtaacaga
    26521 ctcaatctga tcttcaacgt aatcgcccaa gttcaggctt tcatcttcat aacgtgcggc
    26581 ttcaagggtg atttccttgg tcggctgggt gacttcatca acaactaacc agcgacggaa
    26641 ggtgtcaaaa tcaccgcttt tgcgatcgat ctgtacacgg acgtcgatct cttgctcata
    26701 ttttttcttt gttgctgtcg ccagcgcgct ttccaatgct tcgaaaatct tctcgcgagg
    26761 tagcgccttt tcattggata cggcttcaac tacagccaaa atttctttgt tcatcgcggg
    26821 cttttcacct catccagact attaaaagtg gggaaccagg ttcgccttct ggatattact
    26881 cagcgcgaac acttcatctt taccttcgac ggtaactgtg atcatttcac cgtctaccgc
    26941 tttgataacg ccctgccatt tacgacggtt ttgtaccgcc atacggagaa ccagagtcac
    27001 ctcttctccg acaaaacggg cgtagtgttc agccgtgaac agtgggcgat cgagacccgg
    27061 tgaggagact tccaggttat aagcaacggt gatgggatct tcaacatcca gcacagcact
    27121 tacctggtgg ctcacatcag cacaatcatc aacattgatg ccatcttcac tatcaatata
    27181 gatgcgcagt gtggatgtgc gaccgcgaat aaattcgatg cctaccagtt caaaacccag
    27241 ggcctcaact ggcgcagtaa tcatctctgt taatttttgc tctaatgtgg acaagcccac
    27301 ccccaagaca taaaaaaagg gcctaaagcc cagttattct gtagtcagat aacaaaaaac
    27361 cccgataaat cggggcttta tataactgaa ccctataacc gcaactgcgg tctggagcac
    27421 tttccagaag gattttttca aatcccacta cgaaggccga agtcttcaca gtatatttga
    27481 aaaaggactc taagggaaag tggttgcggg ggccggattt gaaccgacga ccttcgggtt
    27541 atgagcccga cgagctacca agctgctcca ccccgcgcct gaaacgtggc aaattctact
    27601 cgttttgggt aaaaaatgca aatactgctg ggatttggtg taccgagacg ggacgtaaaa
    27661 tctgcaggca ttatagtgat ccatgccaca ttttgtcaac gtttattgct aatcacgtga
    27721 atgaatatcc agttcacttt catttgttga atacttttgc cttcccctgc tctcccttaa
    27781 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
    27841 tgaaaactca tttattttgc ataaaaattc agttagagcg gaaatccagg ctcatcatca
    27901 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
    27961 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
    28021 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
    28081 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
    28141 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
    28201 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
    28261 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
    28321 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
    28381 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
    28441 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
    28501 acagactcca acatgcttgg tgcaacgcat gaagcgaagg atctggaata cctcaactcc
    28561 agcgtcaaaa tcgtcaaccc gattatgggc gtgaaattct gggatgagag cgtgaagatc
    28621 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtggcgct gaacggtaaa
    28681 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
    28741 ctgggcatga gcgaccagat tgagaaccgt atcatcgaag cgaaaagccg tggtatttac
    28801 gaagctccgg ggatggcact gctgcacatt gcgtatgaac gtctgttgac cggtattcac
    28861 aacgaagaca ccattgagca gtatcacgcg catggtcgtc agttgggccg tctgctgtac
    28921 caggggcgtt ggtttgactc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
    28981 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
    29041 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
    29101 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa tctggatatc
    29161 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctttc ctcctctgcc
    29221 acttcaggcc tgccgcaggt ggagaatatg gaaaacaaag gccagtaatt cgacatcaac
    29281 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
    29341 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
    29401 tcacttcctc aagcgtttgc ggctgttccg gctttgttac ccccatccac gcattaataa
    29461 ggtaattatt gtaagctgtc gaaaagatat tgttttccgt tgtgcgatcc acgccatccc
    29521 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
    29581 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
    29641 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
    29701 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
    29761 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
    29821 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
    29881 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
    29941 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
    30001 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
    30061 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gcttgattaa
    30121 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
    30181 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
    30241 ttgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
    30301 gatgctcttt cgctgctaaa gcaaaatagt tgagattgaa aaacggcgta taggtagcaa
    30361 atcacgacgc tagtatatat ggactgaacg cttttttatt ttttgcatct ttataagcaa
    30421 attgacaagc ggaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
    30481 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
    30541 aagcaaaagc acatagataa gggatataca tccccagcat tttcacaact tcatccggat
    30601 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
    30661 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
    30721 ggcgaagcgg aagggatgat atacgtttaa caaccaggat ggtcaggacg aagaacagca
    30781 atcgctgaac catatgcgct ccggaagagg cgactaagtt cgtaatacca aaaacaataa
    30841 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
    30901 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
    30961 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
    31021 aaatgatgcg accattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
    31081 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
    31141 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
    31201 gcagtaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
    31261 cgagtaaagc aactggacga gatacagata cctgacaacc attcatcaga ccaggaccaa
    31321 aacgaaaaga gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
    31381 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
    31441 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
    31501 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctctatt
    31561 ggttttgttg ctattgatgt tacccagcac cagactgatg atgaagaata acgttgccag
    31621 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
    31681 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
    31741 accaacaagg ccaattgcca caataaggaa aactactaaa agagcttcgt acataatcaa
    31801 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
    31861 tctttcccac tgaagcgggt gtgaatacta accaaaacga atgaccttcg caagggcaat
    31921 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gccgagaaaa
    31981 aaggcgacaa tcgccgcctt tttagccagt tatctaacgc tttaaacggc ttttactgca
    32041 tcggcgatgc ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc caccatcacg
    32101 cgaattaacg gttcggtgcc ggatttacgc agcaacacgc gtccacggtt gcccagcgca
    32161 gcttcaacct ctgcggtcac ggctttaact gactcatgct caagcggatc gccgctacct
    32221 gcggtgtaac gtacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
    32281 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
    32341 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
    32401 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
    32461 ggaattccta actgtttcag cgccagttca agccccatgt tgctcatcaa tgtacccaca
    32521 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
    32581 tcgccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
    32641 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
    32701 gtagccccca cttcggcatt gatgtttaca ccgtttggct cacaaccgat agcgataacg
    32761 ttcgccccca gttcgcgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcacaa
    32821 tccaccacaa tcttcagttc actgaggcta agttcgttcg ggaacgtggc tttgcaaaac
    32881 tcgatatagc gacccgcggc atcaacgata cggctggctt tacccagttc tgccgaatca
    32941 acgcagctga tctccttttc catttccgct tcgatggcct cttctaccgc atccggcagt
    33001 ttagtgccgt cgatagagaa gaatttaatc ccattatcgt agaacgggtt atgcgatgca
    33061 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
    33121 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
    33181 gactccagca tatagccaga aatacgcgtg tctttaccaa taataatctt acgggagccg
    33241 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
    33301 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
    33361 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
    33421 tttgacgtca tgaacacgaa tgatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
    33481 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
    33541 tcgtgacata cccaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
    33601 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
    33661 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
    33721 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
    33781 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
    33841 aagggagcgg atatcattaa taatgtgagc gccaactttc gctgactcac ggatgacttc
    33901 tggtttggat gtatcgaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
    33961 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
    34021 ctcgccacca acgtcaatga tcgtcgcgcc agcattgatc atcagattcg catgtttcac
    34081 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
    34141 gatccccatt acgtgaggat ggctaaggtc cagtgaagta ccctgggcaa agagtttcat
    34201 tatgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
    34261 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
    34321 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
    34381 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
    34441 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
    34501 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
    34561 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
    34621 ttcgctttcg ctacgctacg gccgaggaat acttcacctt cttcttccgc gtacagcagt
    34681 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgtgccag gttggtcgca
    34741 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
    34801 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
    34861 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
    34921 gtcactttat gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
    34981 tcgtggtaag ccgttgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
    35041 cccatcatga ttttgtcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
    35101 ccacgagcag cgaacagtgc cgcttcgttc accaggttcg ccaggtcagc accggagaaa
    35161 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
    35221 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccgac cacaacctgg
    35281 cggtcgaaac ggccaggacg cagcagggcc gggtcgagaa cgtccggacg gttagtcgcg
    35341 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
    35401 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
    35461 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
    35521 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
    35581 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcaacgt tttaccggta
    35641 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
    35701 ctcggctcgc gcagatactc taccagttca gcaacttctt cttttgcttc gtcgcagccc
    35761 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
    35821 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccag
    35881 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
    35941 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
    36001 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
    36061 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
    36121 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
    36181 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
    36241 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
    36301 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttct
    36361 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
    36421 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
    36481 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggctcgg
    36541 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
    36601 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
    36661 ccctgaagaa agtccacacc aacgatagga tccataggta aaagatcgca agcgatgatg
    36721 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
    36781 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
    36841 ttaaaccagg cacgggaacg taatcccttt ttctgtgcct gttgaacata tttatcgcta
    36901 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
    36961 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
    37021 atatgggaga tggcggtaga atgacccgtt ttcaatccca acgtaagcaa aaaatacgat
    37081 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
    37141 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
    37201 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
    37261 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
    37321 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
    37381 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
    37441 gccacgctct gttcgttgat aaaaggccgc agagcggcct ttttcctttc tttacaatac
    37501 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
    37561 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
    37621 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
    37681 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
    37741 aaaaataacg cgcccattgt tgggcatttt ggtgacatca atcacctgcg cgtttgacag
    37801 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    37861 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    37921 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    37981 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    38041 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    38101 aaaaataccg acccgggtac aagtcccagg tcagctacaa ttcacatttt gatggtcatt
    38161 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    38221 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    38281 gaccagctgt atagcgttca gtgttcaggc cgcgaatgtt gatgagtacg ttactcaact
    38341 ccccgcaggt gccaaccttg ccctgatggt gcaaaaagtc ggtgcgtcgg cccccgctat
    38401 tgattaccac agtcagcaga tggcgctgcc cgccagtacc cagaaagtga ttactgcgct
    38461 ggcggcgttg attcaactcg gccccgattt tcgtttcacc acgacgcttg aaaccaaagg
    38521 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    38581 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    38641 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    38701 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgctgccg ccatagttga
    38761 ccgcaactgt ttctccgtct cgctctacag tgccccaaag cctggtgata tggcttttat
    38821 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    38881 ttctgccgaa gcgcaatact gcgaactgga tgtggtgcca agcgacctga accgctttac
    38941 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    39001 tggagccagc tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    39061 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    39121 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg acaacatgat
    39181 cgccgatacg gttttccgca tgatcggcca tgcgcgcttc aatgtgcctg gaacatggcg
    39241 ggctgggtcg gacgccgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggtaa
    39301 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    39361 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    39421 gccgcttgcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    39481 ggatggcaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    39541 cattaccaca gcgagcgggc aacgaatggc atttgtacaa tatctttctg gctatgcagt
    39601 aaaacccgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    39661 caaagatatt tatcagaaca attagtcaaa aagaaacccc ggcgcatggc cggggtttca
    39721 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtgaacgcct tatccggcct
    39781 acaaaatcgt gcaaattcaa tatattgcaa tcatcttgta ggcctgataa gtgtagcgca
    39841 tcaggcttat ttggcattgg tcattagtga ttaacgcttg taaatgaact caacgccttc
    39901 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    39961 aagctgctga cggtgataat catcccacat gaattcgact ttctctggct gtttcgcttc
    40021 ttcagcctgc acgaccgggt tttcaatgat aaaggtcatc acatcccagc agagatcttt
    40081 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    40141 agcgatcact ttcgctttct cttcggcttc tgccttatcc agcagatcga tcttgttgaa
    40201 gactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    40261 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    40321 caggacgcgg caacgttcca ggtgcttcag gaagcgaatg cccagacctg cgccttccgc
    40381 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    40441 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    40501 cgccgatacc gcacgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    40561 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcgcccggcg taccgttagt
    40621 tttctgccgt ggtgtacggt taactgacga tttgaaacgg gtattgccca gaccgtgcca
    40681 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcaccca tggtttcacc
    40741 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    40801 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    40861 aaaacgataa tcgataagcg tgttcaggtt ctcgtcggct tccatccata cgtcaccacc
    40921 atcaccgccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    40981 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    41041 cattttattc tccgtaaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    41101 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    41161 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    41221 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    41281 cacctgcgct gcctgccagc gagccatcgc ttccgccaag gcgccatatc ctaccaaggt
    41341 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    41401 aggctttgcc agagggaaga gcgcgattgt acataaagtg tacagtaaaa acaggatctg
    41461 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    41521 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    41581 gaccagactg gtgttaaaaa acatcaccag accgctcagg agcatcaatg cccctacaac
    41641 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    41701 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    41761 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    41821 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    41881 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    41941 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtaagcg ccaaaagaat
    42001 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    42061 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    42121 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    42181 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    42241 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    42301 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaagc gtttagcttc
    42361 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    42421 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatggccc
    42481 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    42541 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    42601 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    42661 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    42721 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    42781 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    42841 agtgctacag tcaatacacg aagaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    42901 cattttttca gtacaattac cctatattcc taaccataaa ccctaagttg cctttgttca
    42961 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    43021 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    43081 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    43141 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    43201 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    43261 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    43321 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    43381 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    43441 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    43501 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    43561 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    43621 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaacgtcgg
    43681 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    43741 accagaacag gcacagatga tccgtaccgc catcgaacaa ggtaacggtc gccatcttct
    43801 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgcgc
    43861 cgaggaagaa gcagacaaag ccatcgcagc gttacaggtg ctcccggaca ccccttggcg
    43921 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    43981 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    44041 ataacacctg aaaaaagggc gataaatctt tatatattct gaatattcac actctttaca
    44101 ggaacttttt agcgcaatag accatcagga gtatagtgat gcccgacaga agaagtgttc
    44161 tgaatgaaag cgactaactt aaggagtgag gaaagatgga aagtaatttc attgactggc
    44221 atcccgctga catcattgca ggtttgcgca agaaaggaac gtcaatggcg gcggaatctc
    44281 gcagaaatgg tttgagttcc tcaacgctgg caaacgcatt atcgcgccca tggccgaaag
    44341 gagagatgat tattgcgaaa gccctgggaa ctgacccctg ggttatctgg ccatcacgct
    44401 accatgatcc gcagacccat gagtttatcg acagaacgca gttgatgcgt agctacacta
    44461 aaccgaaaaa atgagtggtc tggcggtagc cccgcgaacg gggctgccag ctctcagacg
    44521 attactcgcc tttcacacgc tcaatatttg cacctaaagc gcgcagtttg tcttcaatgc
    44581 gttcgtagcc acgatcgatg tgataaatac gatcaaccac cgtcgtccct tccgcaatac
    44641 agccagccag caccaggctt gctgatgcac gcagatcggt tgccataacc tgtgcaccag
    44701 aaagtttttc aacaccatga caaataacgg tattgctttc gatttcggcg tgcgcgccca
    44761 tacggctcag ctctggcaca tgcataaagc ggttttcaaa gaccgtttcg gtgatgaacc
    44821 cggtcccttc agccaccagg ttcaacagcg tgaactgggc ctgcatatcg gtcgggaatg
    44881 ccggatgcgg cgcggtacgt acgttaacag ccttcggacg tttgccatgc atatccaggc
    44941 taatccagtc ttcgccgact tcgatgtccg ctccagcatc acgcagtttc gccagcacgg
    45001 cgtccagagt atctggctgc gcgttacggc agataatttt gccacgagag atcgccgccg
    45061 ccaccaggaa agtaccggtt tcgatacgat ccggcagcac gcgatagaca ccgccgccta
    45121 aacgttccac accttcgatg acgatacgat cggtgccctg accgctaatt ttcgcaccca
    45181 gcgtaatcag gaagttcgcg gtatcgacga tttccggttc acgcgctgcg ttttcaataa
    45241 tcgtggtgcc ttccgccagg gttgcagcac acatgatggt caccgttgcg ccaacgctga
    45301 ctttatccat cacgatatgt gcgcctttca gacgaccatc gacggaagct ttaacgtaac
    45361 cttcttccag tttgatggtc gcgcctaatt gttcgaggcc agaaatgtgt agatcaaccg
    45421 gacgcgcacc gatcgtacaa ccgccaggta gtgaaacttg cccctgacca aagcgcgcta
    45481 ccagcggccc cagcgcccag atagaagcac gcatggtttt aaccagatcg taaggtgcgc
    45541 agaatacatt aacgtcgcgg gcatcaatat gcacagaacc attacgttct actttcgcac
    45601 ccagctggct tagcagtttc attgatgtat cgacgtcttt cagtttcggg acgttctgga
    45661 tctctaccgg ttcttccgcc agtagtgcgg caaaaagaat aggcagagca gcatttttag
    45721 cgccggaaat tgtgacttcg ccctggagct tcgttggccc ctgaacacga aatttatcca
    45781 tttagtttgt tctcagttaa caattcatat ccgctaccgg cgaatcgccc atagctcaaa
    45841 agccgttcag tttgcgatcg cgcgcccact ccgcaggggt atacgctttg atcgacacag
    45901 catgaatgcg gttatccgca atatattcca tcagcgggcc atagaccgtc tgctgttttt
    45961 taacccgact cataccgtca aacaactcac ccacggcaat aacctgaaag tggctgccat
    46021 cgccggaaac gtggacttcc tggagggaga gagcgttcat caacacgctc tgaatttcat
    46081 tattttccat gggatcttca atcatcagtt aataaaccag cgaaacatct tagagcaaag
    46141 ttgcgctggc ataaataagc aaaaagcctc gctgataaat cagacaaggc tcgacttgca
    46201 ggcaggtttg ccggacaggc ggttaacgcc atatccggcc tgaaaaaatt taacgaggca
    46261 gaacatcagc aggcaaatta tacaatttcg cgagggtata cactttgtcg tttaccccct
    46321 gaagcgtcac gttgttgccc tgctttttcg ccagatcgat aagatggagc agcagtgcca
    46381 gtccccccgt atccacgcgg gacacacggc taagatcgat gcaggtaatc cccttcaccg
    46441 cctcctcacg catttcccaa agcggtagca gaacgtcctg atccagctct ccggataacg
    46501 ccagcgtgtc acccgtctgc atccagctca gtgactcgct cattattttt tctcttccag
    46561 agtgattttc tgttgagaaa tcgatttcag ttgcgcagtc aggccgtcga tacctttggt
    46621 acgcagcagc gttccccact cgttttgttt ggtggtgatc atactgacgc cttcagcaat
    46681 catgtcgtaa gcttgccagt ttcccgtctg ggagttttta cgccactgga agtccagacg
    46741 caccggcgga cggccattcg ggtcaataat ggtaacgcga ataggcacaa tggttttatc
    46801 gcccagcggc tgttctggcg caatctgata ggtttgaccg tgatacatcg ccagcgcctg
    46861 accgtaagcc tgcttcaggt actcacggaa agcggcaaag taggcttcac gttgagcagg
    46921 ggtcgcactc ttgtaatact ggcccagcac cagcgcaccg gcgtatttca cctgtacgta
    46981 tggcagcagt tcctgatcaa caatggtacg cagataatcc gggttggccc gaatttgcgg
    47041 ttgctcattc ttcaggcgat cgaacgtttt ctgcgccgcc tcgtccatca gcttataagg
    47101 attggtctgg tctgccgcgg ttgccgcact cagaggtgca atcaccagca aagcgaccat
    47161 cattaaacgt ttaaacatgc gtcggttctc ctgaaattat ttcgttgtac ccacaggttc
    47221 agtggtttca ttattacctg gcgcagcagc tggcgcatcg ccactattct tattgtcatc
    47281 gcctttacta ccgtaaagga actgaccaat gagatcttcc agcaccatcg cagacttggt
    47341 gtcctgaatt gtatcgccat ccttcaggat agcagtcccc agttccgggt cttcaaaacc
    47401 gacgtttaat gccagatatt gttctcccag caggccggaa gtacgaatgc tcagcgaact
    47461 ggtatcagga atgtggttat aacgttgttc aatttccagc gttacgcgcg gcagataggt
    47521 tttcgggtcc agcgtaatat ccgccacccg gcccacaaca acaccaccaa tgctgaccgg
    47581 agagcgcgct ttcaggccgc caatgttatc gaacgtcgca taaagcgtgt aggtcgattc
    47641 agtacgtatg gacgtcacgt tcgccgcctt caggcaaaca aacagcgccg ccagcagtgc
    47701 tgctaataaa aagataccca cccaaatttc attttttttc gtttgcatga actcaattcc
    47761 caaacatcaa tgcggtcagc acaaaatcca gccccagaac agccagagac gagtggacaa
    47821 cggtgcgagt ggttgcccgg ctaatcccgg cagacgtcgg gatggcgtcg tagccgttaa
    47881 acaacgaaat ccacgtcacc gtgatggcga acaccacgct cttaatcaga cagttgacca
    47941 gatccatacg ccagtcgacg gcattttgca ttgccgacca gaagaacccg ctatcaatgc
    48001 ctttccaact gacgccgacc agcgatccgc cccagatccc cacggcgacg aagataaccg
    48061 tcaacagtgg taatgaaata accccagccc agaaacgggg agaaataacc cgacgcagcg
    48121 gatccaccgc catcatctcc atactggaga gttgctctgt agcgcgcatc aggccgattt
    48181 ctgcggttaa cgccgaacca gcacgcccgg caaacaacag cgcggcaacc accggcccca
    48241 gttcacgcag tagcgataac gccaccagca tacccagact ggtttccgcg ctataagtgg
    48301 tcagaaccag ataaccttgc agccccaaca ccattccgat gaacacgcca gaaactacaa
    48361 taatcagcat cgacaggacg ccgacattat agagctggcg caccagcagc ggcgcatgtt
    48421 tgcgaaattc cggtttgccg accagcgcat tgaataacat taacccggcc cgcccgaacg
    48481 ttctcagggt tttaatccct ttatgtccga gcgacgccag cgcatttaac agcatgagtg
    48541 gcttaactcc ctggtaaaag atcagcgtga taatcgccgg caggatagcg gaacggaaca
    48601 ggcccgtcag ctatcccgtc cagaaactga cgtacgcgcg gatcaggatt cgtctgcaac
    48661 gcctgggcac tgccatgagc gacaattttt ttgtccgcca ggatccaggc gtgatccgca
    48721 atacttaaca cttccggcac atcgtgagaa accaccacac aagtcacgcc cagcgcgctg
    48781 ttcagctcag aaatcagctt caccagtacg cccatggtga tgggatcttg cccaacaaaa
    48841 ggttcatcaa acatgatgag atccggctcc agcgcaatcg cacgcgccag cgctgcacgc
    48901 cgcgccatcc caccggaaag ttcagaaggc attagtttag ccgctccacg cagccccacg
    48961 gcctccagct tcatcatcac cgtactatgc aacaatggcg cgggaagttg ggtatgttcg
    49021 cgcagtggat aggcgacgtt gtcaaatacg ttcatatcag tgaacaacgc cccggactga
    49081 aataacatgc tcatccgttt acgcactgta tacaggcgcg aacgagacat cgccggaata
    49141 ttctcaccat cgaaaaggat ctcaccatga tctggtgcga tttgcccgcc aatcagacgg
    49201 agtagcgtcg ttttaccgat gcccgatggc cccatgatcg ccgtgatctt ccctcgcggc
    49261 acggtcaggg aaatattatc gaagatgcag cgattgccac gcgtaaaact gacatcgcgc
    49321 atatcgacta aattcgccac agactgctcc ataattcacc cttcgtcttg cgttgatttt
    49381 ctaagcatgg cgctcaattt aaccttgaac ccaacatatt tacagaatat tacccgccgt
    49441 ggttagcgaa agctggcatt tgttttactt tttagccgca taaagtcaaa attaagaatc
    49501 cgttacggct ttctgaaaat cttcagcgga ccggcgagta tacctgaaga aaggacgtta
    49561 gatgctttta gctacggcac tgttaattgt tggtttactt ttggtcgttt acagtgccga
    49621 ccgcctggtt tttgccgcgt ctattctttg ccgaaccttt ggcatcccgc cgctgatcat
    49681 cggcatgacg gtggtcagta ttggtacatc gttaccagaa atcatcgtct cgcttgccgc
    49741 gtctctgcac gaacaacgcg atttagccgt tggtacagcc ctcggctcaa acattatcaa
    49801 tatattgctg atcctcggtc tggctgcgct ggttcgtcct tttaccgtcc attccgatgt
    49861 tctgcgccgt gaattaccct taatgttgtt ggtcagcgtg gtggccggtt ccgtactcta
    49921 tgacggacaa cttagtcgaa gcgatggtat ctttctcctc tttctggctg tgctatggct
    49981 gctgttcatt gttaaacttg cacgtcaggg gactgacagc ctgaccagag ag
//
LOCUS       DAJTUN010000013        48281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTUN010000013 DAJTUN010000000
VERSION     DAJTUN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.013"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            188..481
                     /locus_tag="OO470_000733"
     CDS             188..481
                     /locus_tag="OO470_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6250081.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO470_000734"
     CDS             complement(522..1712)
                     /gene="nepI"
                     /locus_tag="OO470_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6250082.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1923..2375)
                     /locus_tag="OO470_000735"
     CDS             complement(1923..2375)
                     /locus_tag="OO470_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6250083.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO470_000736"
                     /gene_synonym="yicO"
     CDS             complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="OO470_000736"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6250084.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3937..5703
                     /gene="adeD"
                     /locus_tag="OO470_000737"
     CDS             3937..5703
                     /gene="adeD"
                     /locus_tag="OO470_000737"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6250085.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO470_000738"
     CDS             complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="OO470_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6250086.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO470_000739"
     CDS             complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="OO470_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6250087.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO470_000740"
     CDS             complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="OO470_000740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6250088.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO470_000741"
     CDS             complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="OO470_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6250089.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO470_000742"
     CDS             complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="OO470_000742"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6250090.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO470_000743"
     CDS             complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="OO470_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6250091.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO470_000744"
     CDS             complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="OO470_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6250092.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13525..13614
                     /gene="tisB"
                     /locus_tag="OO470_000745"
     CDS             13525..13614
                     /gene="tisB"
                     /locus_tag="OO470_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6250093.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13894..15078
                     /gene="emrD"
                     /locus_tag="OO470_000746"
     CDS             13894..15078
                     /gene="emrD"
                     /locus_tag="OO470_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6250094.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15086..15583)
                     /locus_tag="OO470_000747"
     CDS             complement(15086..15583)
                     /locus_tag="OO470_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6250095.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15580..15942)
                     /locus_tag="OO470_000748"
     CDS             complement(15580..15942)
                     /locus_tag="OO470_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6250096.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15932..16279)
                     /locus_tag="OO470_000749"
     CDS             complement(15932..16279)
                     /locus_tag="OO470_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6250097.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16388..16801
                     /locus_tag="OO470_000750"
     CDS             16388..16801
                     /locus_tag="OO470_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250098.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16848..18290)
                     /locus_tag="OO470_000751"
                     /pseudo
     CDS             complement(16848..18290)
                     /locus_tag="OO470_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18325..20040)
                     /locus_tag="OO470_000752"
     CDS             complement(18325..20040)
                     /locus_tag="OO470_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6250099.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20207..21100
                     /locus_tag="OO470_000753"
     CDS             20207..21100
                     /locus_tag="OO470_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6250100.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21097..21911)
                     /locus_tag="OO470_000754"
                     /pseudo
     CDS             complement(21097..21911)
                     /locus_tag="OO470_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21911..23528)
                     /locus_tag="OO470_000755"
                     /pseudo
     CDS             complement(21911..23528)
                     /locus_tag="OO470_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23823..24497
                     /locus_tag="OO470_000756"
     CDS             23823..24497
                     /locus_tag="OO470_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6250101.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24536..26197)
                     /locus_tag="OO470_000757"
     CDS             complement(24536..26197)
                     /locus_tag="OO470_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6250102.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26393..26821)
                     /gene="ibpB"
                     /locus_tag="OO470_000758"
     CDS             complement(26393..26821)
                     /gene="ibpB"
                     /locus_tag="OO470_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6250103.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26933..27346)
                     /gene="ibpA"
                     /locus_tag="OO470_000759"
     CDS             complement(26933..27346)
                     /gene="ibpA"
                     /locus_tag="OO470_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6250104.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27340..27412)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27651..27983
                     /locus_tag="OO470_000760"
     CDS             27651..27983
                     /locus_tag="OO470_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6250105.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27985..29205)
                     /locus_tag="OO470_000761"
     CDS             complement(27985..29205)
                     /locus_tag="OO470_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6250106.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            29306..30406
                     /gene="cbrA"
                     /locus_tag="OO470_000762"
     CDS             29306..30406
                     /gene="cbrA"
                     /locus_tag="OO470_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6250107.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30367..31704)
                     /locus_tag="OO470_000763"
     CDS             complement(30367..31704)
                     /locus_tag="OO470_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250108.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31779..32927)
                     /gene="dgoD"
                     /locus_tag="OO470_000764"
                     /pseudo
     CDS             complement(31779..32927)
                     /gene="dgoD"
                     /locus_tag="OO470_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32924..33541)
                     /gene="dgoA"
                     /locus_tag="OO470_000765"
     CDS             complement(32924..33541)
                     /gene="dgoA"
                     /locus_tag="OO470_000765"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6250109.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33525..34403)
                     /gene="dgoK"
                     /locus_tag="OO470_000766"
     CDS             complement(33525..34403)
                     /gene="dgoK"
                     /locus_tag="OO470_000766"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6250110.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34400..35089)
                     /gene="dgoR"
                     /locus_tag="OO470_000767"
     CDS             complement(34400..35089)
                     /gene="dgoR"
                     /locus_tag="OO470_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6250111.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35367..36023
                     /locus_tag="OO470_000768"
     CDS             35367..36023
                     /locus_tag="OO470_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250112.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36069..36881)
                     /gene="yidA"
                     /locus_tag="OO470_000769"
     CDS             complement(36069..36881)
                     /gene="yidA"
                     /locus_tag="OO470_000769"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6250113.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36995..37393)
                     /locus_tag="OO470_000770"
     CDS             complement(36995..37393)
                     /locus_tag="OO470_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6250114.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37633..40047)
                     /gene="gyrB"
                     /locus_tag="OO470_000771"
     CDS             complement(37633..40047)
                     /gene="gyrB"
                     /locus_tag="OO470_000771"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6250115.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40076..41149)
                     /gene="recF"
                     /locus_tag="OO470_000772"
     CDS             complement(40076..41149)
                     /gene="recF"
                     /locus_tag="OO470_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6250116.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41149..42249)
                     /gene="dnaN"
                     /locus_tag="OO470_000773"
     CDS             complement(41149..42249)
                     /gene="dnaN"
                     /locus_tag="OO470_000773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6250117.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42254..43657)
                     /gene="dnaA"
                     /locus_tag="OO470_000774"
     CDS             complement(42254..43657)
                     /gene="dnaA"
                     /locus_tag="OO470_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6250118.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43954..44034
                     /locus_tag="OO470_000775"
     CDS             43954..44034
                     /locus_tag="OO470_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250119.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44264..44404
                     /gene="rpmH"
                     /locus_tag="OO470_000776"
     CDS             44264..44404
                     /gene="rpmH"
                     /locus_tag="OO470_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6250120.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44421..44780
                     /gene="rnpA"
                     /locus_tag="OO470_000777"
     CDS             44421..44780
                     /gene="rnpA"
                     /locus_tag="OO470_000777"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6250121.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44744..45001
                     /gene="yidD"
                     /locus_tag="OO470_000778"
     CDS             44744..45001
                     /gene="yidD"
                     /locus_tag="OO470_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6250122.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45004..46650
                     /gene="yidC"
                     /locus_tag="OO470_000779"
     CDS             45004..46650
                     /gene="yidC"
                     /locus_tag="OO470_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6250123.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46756..48120
                     /gene="mnmE"
                     /locus_tag="OO470_000780"
     CDS             46756..48120
                     /gene="mnmE"
                     /locus_tag="OO470_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6250124.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11978 a        13053 c        12204 g        11046 t
ORIGIN      
        1 agcggtaagg tcgctgcggt ttaaaaaacg aaacgctatc gataagaata aaaaggaata
       61 aaagtggaat ataaagtctg gcacttctta ctgaccacgc aagcgcgttt cgtacagcat
      121 gatgagagcg atgaaagcaa actacactta tgcttcattc gatacacatt tgtaaaaggt
      181 tgaatccatg aaaccaacga cactactgct tattttcact tttttcgcta tgccaggcat
      241 cgtatacgcc gagtcgcctt ttagctcact gcagtccgca aaagagaaaa ccacggtatt
      301 acaagattta cgtaaaattt gcacgccaca tgcgtcatta tcagatgaag cgtgggaaaa
      361 gttaatgttg tcagatgaaa acaataaaca acatatacgc gaggccatcg tggcgatgga
      421 acgtaataat cagagcaatt attgggaagc gttgggtaag gtagaatgcc cggatatgta
      481 agaacagcaa caactactga ccaacgcttc tttacctgat atcaggattt cttcattttc
      541 acctttgcag taaccaacaa tgcagtcagc aacatcaatg tgccggacaa catcaacggc
      601 gaagtcagac caatattatc cagcgcataa ccgccgattg ccgcgccaca ggtattagca
      661 agctgaataa ccgccacctg aatagacccg gctttttctg cctgatcggc cagcgagcgg
      721 gtgatccacg ttgaccagcc gacgggaacc aatgcaaaag ttagccccca gataatcgcc
      781 acgccggtag caacgatttt atcgcttccc cacaacgtca gtaccaacgc actcacagcc
      841 agtattaacg gcgcgcctgc taaggccagt tttaccgaac gtttaagaat gaacgacgaa
      901 agcgacgtac caataaagct ggcgatacca aaactcaaca gcaccagcgt taagccatcc
      961 acgccgaatc ccgccaggtt catatacacc gggcgaatat acgtgaagaa agcaaactgc
     1021 ccggcgaaag acatgaagat ggcgatcatc cctgccatca cacccggacg ttgtaataag
     1081 cggaaagtat tttgtttctg atgcgagggt tcgcctggca gtgaaggcaa tgatttgata
     1141 atccagaaaa tacacagcac gcccatcacc gccgccgcat taaagacatt gcgccaaccg
     1201 ataagctcgc ctaaaaaact gcccaacggc gcggcaatca ccagcgcaat agaaaccgcg
     1261 ccgaagatca ccgacagcgc cttcggcacc gtacgcggcg gtaccagacg catggtcagc
     1321 gacgccgaca tcgcccagaa cccgcccagc gccagcccca gacaggcacg accgattaaa
     1381 agcaaactga atgagttagc aaaggaaacc agcaagcagg agagcgtcag caaaacggca
     1441 aacaaaataa caacgtagcg gcggtcagtg gcctgaattg tctgggtaat aaacaaactg
     1501 gcaaacattg ccacaaaggc ggtcacggtc accgattgcc cggcaacccc ttccgaaatg
     1561 cctaaatcct gggccattgg cgtcaacaaa ctgacgggca aaaactcaac gataatcaga
     1621 caggcgacac aaaacgccac cgagaaaacg gctgaccagt tcggtcgggt gatggcatcc
     1681 gcgccgcggt tttcggcaat aaattcactc atggtgttac ccgtggcatg tttcagggga
     1741 aaagccgacc actttaacat tgaaatgtgt gacgaattta acgtttttgc aacttttacg
     1801 gtgttgcttg agctggagtt aagatcgaaa cggaacaggg caaagattac cggatgcggc
     1861 gtgaacgtct tatccacccg acaggtcagg cgcggtctgt aggctgataa gacgaaacgg
     1921 tttcaatgca accagtgaac gccctcggca ggctgaaaga acgcgttata gcgcatctga
     1981 aaagcattaa tatcctgcat ttcaggttcg gtttcacgca gaaaacccag cgccagcctg
     2041 acctgagcat cggtaatcgg cgcggaaagg cgggcttttt gtaaaatccg gtgccattgc
     2101 agcaggttct gttcgctatc atcgacaatg acaatttgcc agataagcaa cacctgcgcg
     2161 gcgttttgca ggtgcgactc atccgggcga tgcaaataac gtaaaaattc gtcaccggct
     2221 gactttccca tttgatcgac catcgactcc acgggtacga catcgatatt cagccgatcg
     2281 ctgagacggc gaatcgcttt tctggccccg gatgtcagca cccgaaaacc aaccacaaac
     2341 actaccgcca gcgtggcgag cattatccag atcatgagtt ctcctgcgta atcagaactc
     2401 atgatatctg gaaaccctcg ccagttacta atccaccaga ataatcttca atgcgaacag
     2461 agctgcgacc accaccacac acaggttcag atcgcgccag cgcccggtgc acactttcat
     2521 gatgcagtac gacataaagc cgagtgcaat cccttcggtg atcgagaaag taaagggcat
     2581 catcaccgtg gtaataaacg caggcaccga ttcggtaaaa tcatcccagt taacgcgcgc
     2641 caggctcgaa gtcatcagca cgccaacaaa gattaacgct ccagcggttg cgtaaggagg
     2701 aaccatcgcc accagcggtg agaagaacat aactaacagg aacataacgc cgaccacgac
     2761 agccgttagc cccgtgcgac caccgactgc cacaccagaa gtactttcaa tataggcagt
     2821 aacagacgag gtaccgataa atgcgcctgc caccgaactg acgctatcaa catacagcgc
     2881 cttattcata ttggggaatt taccgttgct atctattaag cccgctttat cagttacacc
     2941 aattaatgtt cctgatgaat caaataggtt gatcagcata aaggagaaaa tgataccggc
     3001 gagttcaagt gttaacgcgc cgctcaaatc tacttcacca atgacgccgc taatatcagg
     3061 cggaatggaa tagacgccgc taaaatgaac atcaccgaaa aataatccac agcaagacgt
     3121 caccacaata gaaaccagca ccgcggcatg aaaatgacgt gatgacaaca cggttataat
     3181 aaaaaaccct aaaataccta acaacacgcc gtgagaactt aaatcgccaa tcatcaccag
     3241 cgtgtcttta ttggcgacaa taacaccagt gttttttaat cccattaagg caataaataa
     3301 tccgattcca ctggtgatac caatacgtaa acttaatgga atgttggaga tcatccagta
     3361 ccggatacga aacagcgtga gcaaaaatag cccaattgcg ccccagaata tcgcgcccat
     3421 cccggtctgc caggagatcc ccatcgcccc cacgaccacg aacgcaaaga aggcgttcag
     3481 ccccattgcc ggagccagcg ccacgggtaa gttagcaaat atccccatcg caatactgcc
     3541 gataccggca atcaaacagg tggtaacaaa caccactttc gggtccattt gtgccgcgcc
     3601 gaggatttgc gggttcacaa aaacaatgta caccatggtt aaaaaagtgg tcatccccgc
     3661 aatcaattct gtgcggacgg tggtcccatg ctgaggtagt ttaaataatc gcgaaagcgt
     3721 ccctgattca ttactcacgt aatcggtatt gtcattattc atttttttgt ccattggagg
     3781 agatttaatc ccttgctttt gtaaaacaaa tgacaacaac ggttcagtga taattatcac
     3841 atttcaattg cacattaata gatattcttt aataatctcg cgacgtttct ttatgataaa
     3901 taataatcaa attgataaaa tcaaaatgag aaaaatatga ataattccat taaccataaa
     3961 tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg tggcgacgct
     4021 gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgc cggagaaatt
     4081 tccggcccaa ttgtgattaa aggacgttac attgccggtg ttggcgcaga atacgctgat
     4141 actccggctt tgcagcggat tgatgctcgc ggcgcaacgg cggtgccagg gtttattgat
     4201 gctcacctgc atattgaatc cagcatgatg acgccggtca cttttgaaac cgctaccctg
     4261 ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt gatgggagaa
     4321 gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag
     4381 gtcagctctt gcgtgcccgc tctggaaggc tgcgatgtta acggtgccag ttttaccctt
     4441 gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga aatgatggac
     4501 taccctggcg taattaacgg gcagaatgcg ctgctcgata aactcgatgc atttcgctac
     4561 ctgacgctgg acggtcactg tccgggtttg ggtggcaaag aacttaacgc ctatattgct
     4621 gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg gaaattacaa
     4681 ctcggcatgt cgttgatgat ccgcgaaggg tccgctgcca gcaatctcaa cgcactggca
     4741 ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga ccgtaacccg
     4801 tgggagatcg cccatgaagg gcatatcgat gccttaattc gccgcctgat cgaacaacac
     4861 aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcacg ccactttggt
     4921 ctgaatcacc tcggcttact ggcacccggc aagcaggccg atatcgtcct gttgagcgat
     4981 gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg aaccgattga tgcgcaaacc
     5041 ttacaggcgg aagagtcggc gagactggcg ctgtctgccc cgccatatgg caataccatt
     5101 gcccgccagc cagtttccgc cagcgacttt gccctgcaat tcacgcccgg aaaacgctat
     5161 cgggtcattg acgtcatcca taacgaattg attacgcact cccgctccag cgtctacagc
     5221 gaaaatggtt ttgatcgcga tgatgtgtgc tttattgccg tacttgagcg ttacgggcaa
     5281 cggctggctc cggcctgtgg tttgctcggc ggctttggcc tgaatgaagg cgcgctggct
     5341 gcgacagtca gccatgacag ccataatatt gtgatgatcg gtcgcagtgc cgaagagatg
     5401 gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtggt acgtaacggc
     5461 caagtacaaa gtcatctgcc gttacccatt gccgggctga tgagcaccga cacggcgcag
     5521 tcgctggcgg aacaaattga cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc
     5581 gatgagccgt ttattcagat ggcgtttctt tctctgccag tgatccccgc gctaaaacta
     5641 accagtcagg ggctatttga tggcgagaag tttgccttca ctacgctgga agtcacggaa
     5701 taataaaaaa agcccggcgt catgcagggc aaaagtcacc agttacgttt atgccactgt
     5761 caactgctga atttttttct cgcggcggat tttgcgttct tccattaccg ccactatcgc
     5821 catcaggcag atacaaccaa tcgcggcgat atccagcgcg gcgaaggtgc ctgcccagcc
     5881 ggtaaggccg aataccggcg tcccatcggc aatcattccc agacctaact tggcaaagct
     5941 gtcaccaatc aggtaagcaa aggtgccttt aataccatcg gcagcgccaa tcgctttttt
     6001 aggtacaaag ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc
     6061 caacgcaaag agagaagcca gatagatata ttcgttactg gcatgttgat acacaccgag
     6121 cgtggcgata atcagcgcca gcgcgatgca ggccaccagg ccacggcgac cgttcgccag
     6181 gtcagagagc cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa
     6241 gccctgaatc gccaccgctt tagagagttt cagttcctgg aacgcgtata cggttgacca
     6301 ctggtcgata ccaatacgta ccacatagag gaaaatgttg gcgaagcaca gcagccagat
     6361 cactttgttt ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc
     6421 tgtctctttg tcctcttcgc tgatctcctc gccgaacagt tcttcagctt tgccgaggcc
     6481 ataagattcc ggggagtcgc tgccgtaacg caggccgata aaaccgacaa tcagcgcgat
     6541 aatcgacggg aagataaaca tgccgatgac atggccatcg aacaggtaat ttgccccgaa
     6601 cagcgccaca cctgctgcgc ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag
     6661 gaatgtcccg cgtttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc
     6721 gccggtactc tggaaaaagc cgcttaaggc gtagaaggca atcatcagga acaggctaac
     6781 cgagccgctg cccatactgg cactgaagcc cagcatacaa atggcagaga ggataagcat
     6841 gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag tatgaaacca acgttttacc
     6901 cacgccataa gtgatggaga aacccaggcc gatcatcccc agctgcgtca tgctcaaccc
     6961 gtaggtcgaa atcatatcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt
     7021 caggtagccg ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg
     7081 ccgcacttcg agcggaaggt ccagggtcgg cttgcgaacc tggtttagga aagccagcat
     7141 gggttactcc tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc
     7201 aggcctgaga tagcgtccaa gaaaaacctg gaaaatttct tagttttgcc ggatatgaag
     7261 tgaaaaggtg agatgcatca cgcttcacgc ggtgtctggg cgttcaaaaa gggcagtaac
     7321 agcagtgcgg aaatcccggc ggcgatagag ataaccacaa aaaatccgct ccagtgccag
     7381 gtatcgagca ctttcgccag cggccaacca gcaagcgacg cccccagata agcaaacaag
     7441 ccgacaaacc ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc cgccgccata
     7501 ccaattaaca tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc
     7561 atcacgtagc tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggcg
     7621 gcgaaaatca aattcatcgg cccacggttg ccgttaaaca atttgtccga gccccaaccg
     7681 gctaccagcg caccgataaa tccgcccagt tcaaacatcg tcactgccgt attcgccgtg
     7741 accagatcga cgcccagcgt ctcggacata tacaaattgc cccagtcgtt gatcgccgcc
     7801 cggaccacat agaccagcac atagcaaaac gaaagcagcc agatatacgg attcagcaac
     7861 acatatttgg tgaggatctc tttacgcgtc aaccctgccc cttcttgttg ttgagcaatt
     7921 tccagcgcgt cgtgccgcca ttcaccgacc gccggtaaac ctaacgcctg cgggcgatcg
     7981 cgtagccgcc agcagagaaa aatccccacg actatcgcca tacaaccagc aatcatcatc
     8041 ccggcacgcc agccgtaatg cagcgcgctc gctgccatca caatgggaat gagtgcgccg
     8101 ccgacgttat gcgccgtgtt ccataatgcc caccaaccgc cgcgctcggt acgtgaatac
     8161 caggccgtta acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcagc
     8221 acccagagca cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttcatgatg
     8281 cccgtggcga taagccctat ccccataaaa taacgggcat ttgagcgatc gctgacaatg
     8341 ccggagacaa acttcgacac gccgtaagta atgtaaaaca gggtcgctaa caggccgata
     8401 tcgctacggc tgagcacgcc gttagcaagg atttctggta ccgcggcgtt aaaacttttc
     8461 cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag
     8521 tagcgataac gggcatcaat ttcatgttta tcagtcatta atggcgcatc ggcaggcgct
     8581 ttcagaaacg gcaacatcat cgaaccttag acataacgtt gaggtagaga aacgctgaca
     8641 cgcgtgccgt gcagacagga aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc
     8701 attccggtga ggccaaaacc ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct
     8761 tcaatcacca gcatcaaccg ttcatcctgc tgccagcctt gcagcgtaac cgcgctggca
     8821 tcggcatgtt tcacaatgtt gttcagccct tcctggcaga cacgaaacag cgtcacgcgc
     8881 tggttttcgc ttaatgctga ttcatcgata cgccattcga gatggctgac aatgccgcgc
     8941 ccttccagct ccatttcccg catcagtgag cggatggcct gctccagggt gagatcatcc
     9001 agctggcgcg gacgtaaccg tcctaacaaa cggcgcaccg cgtcgtaaac gcccagcgac
     9061 agttgttcga tgagctgccc gctctgcttc acactggcgt tatctgccgc cagccgctga
     9121 acaatgcccg cctgagtacg aatagcagtg atggtctgac cgatatcgtc gtgcagctca
     9181 cgcgccacgt cgcggcgcac gctctcttcg gtttctaaca aacgttcagc cagatgctga
     9241 ttgcgcgcca gttccttttg tagcgactgg ttaagttcac gcaaccgctg gatgcctgcg
     9301 cccagtaaca accctgtcag actttgcacc agcaacgaga gtaataaatc caccagatga
     9361 tcgcgccagg tttgactggc gatcagcgcg atggcgttca tcaacgtcgc aatcagcgct
     9421 ccttgccagc catagtgcca ggccagcgcg ataatcggca gcgccagaca gaatggcgta
     9481 aagcgcgaca gttcgtccgg caatcccaac tggagccaga gactgataac aaacagcagc
     9541 aagtaccaga ccagatgccg cccgcgccag ttgatgggct gagaaaccag tgacgggccg
     9601 agcggcagcc aggtgttatt ggcgagatag tgccagaaca ccagacatat cggggccagc
     9661 gtcagaccgc cagttaaagt cagcaacagc gcattccacg actctttgcc gtgccaaagc
     9721 cagggcagcg actgcaacaa cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg
     9781 cgccagtcac gctgatggcg atagcgcgag atcagcgcta cgggcagtaa cgtcagtaaa
     9841 ctaccgatca tcaataacgg aaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc
     9901 agcagccact ccgcacccag caatacgggc cagtatccgc gcgggcattg cagcattagc
     9961 cccagacgca gaccaaacgg aaataacagc accgccatat cagggcgctc aaccagatgc
    10021 aggctgatac tccataggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg
    10081 gtaattaagc gggagaacaa cgtcttcatc accagccatc aaacatgcgg cgcgccagct
    10141 ctacgtcgtt actgacgccc agtttttcca tcagattggc gcgatggacg tgtaccgttt
    10201 tcggtgacaa gcccagttcg gcggcaatct ctttcaccgc cattccttgc gccagttttt
    10261 ccgccacctg gcgttcacgt ttggtcagcg ggtcctgacg accggatgcc agtttaatgg
    10321 caatatccgg cgtcagataa cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt
    10381 catccgggct acagcgtttg gaaagaaagc cgcgtgcccc cgcgttaagc gcctgctcaa
    10441 ccagcgcagg actgtcgtga acggagagca taatcgtcgc catacctttc ggcagctggc
    10501 ttagcagctc cagaccggag atatcgggca tggagatatc gcaaatacac acctgcacac
    10561 cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc aaactcggca actacctgca
    10621 aatcaggttc cagccccagc agctgcgcaa agccggagcg gacgatgagg tgatcgtcta
    10681 taagggcaac ggtgatcatg gtcttgtcct ggcgggtaaa aaaaaacgcg cttaccttaa
    10741 cgataagcgc gatgttgttc aagccttgag cggttactga aaaaacaccg cgatcttgtt
    10801 aaacatcgtc ggatcggact gattacgctg cactttcacg acatcttcca gcttagcgat
    10861 ttggcttatc atctgctcca gacgctggtc gtcattgacc agtagccaga tatggctttt
    10921 gtcgctgtcc tgaatcggca gacaaagaat gccttcaacg ttaaaagcgc ggcgggcaaa
    10981 aaggccacaa acgtgggtca ttacgcccgg atggttgcga acggtgagct ccagaattac
    11041 gttgttatga gttgtgtttt gcatggctta ttcccccacc atttcagtat tcgccgcacc
    11101 tggcggcacc atcgggtaaa ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg
    11161 gccagggcga ttgatgattt cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc
    11221 acaggtttcg aggccgaatc cggcggcaat ctgcataaag ttgattttgc ccggataggt
    11281 ggcggcaaaa acgccttgct cgtagaacag actctgttgc tgatgcacca gccccagcgc
    11341 ttcgttgttc atcagaatga ttttgacatc cagctgattt tcactggcgg tcgccatctc
    11401 ctgaatattc atcatcaggc tgccgtcgcc ggagaaacac aacactttgc gatccgggtt
    11461 cgccagcgcc gcgccaatcg ccgcaggcag gccaaaaccc atcgtgccca gcccaccgga
    11521 ggtcagccac tggcgtgggc gattgagcgg ataagcttgc gcggtccaca tctgatgctg
    11581 acccacatcg gtggtgataa tcgcattgtc atcgacacag gcggcaacgg cgttgatcag
    11641 gccgtaatgg cttaatggat cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa
    11701 atccgctacc aactggtgcc actctgcacg cggttgcgct tccacccgcg ggatcaattg
    11761 cgccagcacg tcatcaacat ccgcctgaat cgccacatgc ggctgcttga ttttacccag
    11821 ctctgcgcga tcgatatcga catgaataat tttggcattc ggacagaact gctcggtttt
    11881 gccaatcgcc cggtcatcaa aacgcgcacc gagcacaatc aacaaatccg cctcctgcaa
    11941 aatatagttg gtactgcgca cgccgtgcat ccccagcata cccagcgaca acggatgcgc
    12001 ttttggcagc atgcccagcg ccattaaagt catggtggta ggcagttgcg ctttctccgc
    12061 cagttcacgc acccgcgcag gcgcattgat cacaccacca cccagataaa gcaccgggcg
    12121 tttggcagcg ttaatcattg tagctgcgtc acgaatgctt tcttcgctaa aggcgggtgc
    12181 agcggctttt tctgccaccg cgggctgagc ttcaatctca aaaaccgccg tttgtacatc
    12241 cttaggaatg tctatccaca ccgggctagg gcggcctgat tgcgcaatgc ggaaggcatc
    12301 gctcatgacc tgtgggagct cttcgatatg tctgaccaga tagttgtgtt tggtgatggg
    12361 gatagagatg ccgtaggtgt ccacttcttg gaaggcgtcg gtgccgatca tcgaggcggg
    12421 aacctgacca gtgatgcaaa tcagcgggat ggagtccagc cgcgcatcag caatggcggt
    12481 caccaggtta gtcgcacccg gcccgctaca ggccatacag accgccggtt taccgtcggt
    12541 gcgcgccatt ccctgagcga taaaccccgc gccctgttca tggcgagcca gaatatggcg
    12601 gatttgcgtg ctttggctta aggcgtcgta aacaggcagg atagaaccgc ccggaatgcc
    12661 cgtcacaatc ttaatgccct gctgttccag gaaatgaacg ataaattctg cgccggtaaa
    12721 gcgcttacgc gtcgatgttg tgcccgaact tgccatgctc cagtcctttt cttctgggcc
    12781 gactttccgg gaggtgctta aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg
    12841 aatcgtgtgt tgttccagtc cctacggcgc attgccgacg accaccacca cacgcacgac
    12901 gaccactgcg gcggatggcg cagttggtag tagttttgcg ttgaacatgg aagtagtcat
    12961 tggggacctt gttggttttg tgtttaacaa tatttataca agcacagctt tacaggggag
    13021 acaatggaaa atttttcagc aagggaaaat tgaggggttg atcacgtttt gtactgaatt
    13081 gcagataaca aaaaaccccg ccggagcgag gtttcgtcag ccgcctgcgg ctggtaaccg
    13141 caaagcacac tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat
    13201 ttcggcacga attttgacgt atttagtgca tagttgagta tcgttcacag tttgcgtttt
    13261 gtccaaatat tactgtttat ttatacagta aacttctata atatcactgt acgcaatgtg
    13321 ttatgcgggg gccgcatcgt tacccggcgc actaagtcct ggctgaaacg ggtggtgccg
    13381 tcagcgcctt aaccccgcgt gagcacactg tgttatgtca acaagcacaa cgtttctcct
    13441 tgagataccg cgtgcacaac agctggcaac aggcagcgga aaggtacgtc agctggcagt
    13501 gctcctgaac cacaggagac gcgtatgagc ctggtggata tcgccattct tatcctcaaa
    13561 ctcattgttg cagcactgca actgcttgat gctgttctga aatacctgaa gtaattcaga
    13621 ttcaagtcgc accaaagggg agcgggtaac cgctcccctt ttatatttag cgtgcgggtt
    13681 ggagtcggat gcgatgctga cgcatcttat ccgccctacc atccctcccg gcaacattta
    13741 ttgccgcttt tgtttacata ttctgccgct aaacaattcc ccattcctgg cgtatatctg
    13801 gctaacattc atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt
    13861 attctaatta tttttataaa agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg
    13921 ttattgatgt tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca
    13981 gctattgccg atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg
    14041 ggcgcttatc tgctgactta cggtgtctca cagctgtttt atggcccgat atccgaccgt
    14101 gtcggtcgcc gaccagtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc
    14161 gcggtcacga cctccagttt gacggtattg attgccgcca gcgcgatgca ggggatgggc
    14221 accggcgttg gcggcgtaat ggcgcgtact ttgccgcgtg atttatatga acggacacag
    14281 ttgcgccatg ctaacagcct gttaaacatg gggattctcg tcagtccgtt gctcgcaccg
    14341 ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg
    14401 gttctttgtg ctggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc
    14461 gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt
    14521 aactgttatt tgctgatgct gattggcggt ctggccggga ttgctgcctt tgaagcctgc
    14581 tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt
    14641 attctgccga ttccggcggc attttttggg gcatggtttg ccgggcgtcc taataaacgc
    14701 ttctcaacgt tgatgtggca gtcggttatc tgctgcctgc tggctggctt gctgatgtgg
    14761 atccccgact ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc
    14821 tttttcggtg ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc
    14881 ttcctggcgg gcacggctgg tgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg
    14941 ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg
    15001 accttaatgg gattgttgat cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat
    15061 caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt catcagctcc atgggagaac
    15121 gatgctgctt tatcagatca cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg
    15181 cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg ccgagcgtgc gggcacaggc
    15241 gatgccagtc gcacgcctgg cagtcacggc gcggttgcgg gttttcgctg ccacaccatt
    15301 tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact gatatctgcc cgcacccaac
    15361 actcaaacag ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca
    15421 gatcgccccg gtcatcaaat tcaagatagg gaatgagctg tacggagcgg atgccccgct
    15481 gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagct
    15541 tatgcctgtc ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa
    15601 tacgataagt tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag
    15661 ggagatcaaa aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc
    15721 cattaaattg cgattacggg tgtagtgcca gaggatcagc gccacgatgg cgaggatgcc
    15781 aatcgaaatc cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat
    15841 caacgcgccg tagcccagca tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag
    15901 ccctgggtcg gcaatgcgtc tggctttacg gctatccggc atacaacacc agtcccataa
    15961 cgatcaccac cacaaccatc aatattaagc tgatgattaa taagctgttg gtgtagggca
    16021 ggtcctcttt cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacattg
    16081 ctaacccgcc ggaaaacaga cacaacaaca acgccagcag ttcacgaatg acgggcgtgg
    16141 cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc cagaaatcct agtgcggtac
    16201 ggatccacgc cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc
    16261 caaggcggga aattttcatt gttgctcctt tgcaatttca ctaacgctct atctttagcg
    16321 gtttttttta agaatttcgt ccgcccgttt cctgatatgc ttttgccatc cgctacaagg
    16381 aggtcagatg ggtatcatag cgcaaaataa aatcagttca ttgggcatgc tgttcggtgc
    16441 catcgcttta atgatgggga ttatccactt ttcattcggc cctttttccg caccaccgcc
    16501 aacgcttgaa agcatcgtgg cggacaaaac ggctgaaatt aaaagaggtc ttctcgccgg
    16561 aataaaaggc gaaaaaatta ccactgtgga gaaaaaagag gacatggata tcgataaaat
    16621 cctcgatcaa agcggtatcg cactggccat tgctgcgcta ctttgcgcat ttattggcgg
    16681 tatgcgtaag gaaaatcgct ggggcattcg cggcgcgctg gtttttggta tcggcatcgt
    16741 gtgcagcatt ttgctgatat tcctgatttt ttccttcctg accggcggtt cgctggttta
    16801 agcagcctga ataatgcccg ccgggtgatc atcagcgggc attatcgtca gaatttctgt
    16861 tttttctcct ccaccttcac tccttgcgtc ggtaggccgg tgtcatagtc gcgtaccact
    16921 ggcgaatagc catcttgtgg gcgtgggcga aacgccccca tccagcgcgg tcgtgcatct
    16981 ttacgccacg gacgcagact ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg
    17041 taatccaata aggcgtcatg cattttgctg cgaacgtctg caaaacggat atcatcgatc
    17101 aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg
    17161 ttgagcacca gtttaaagtc atccgtcacc cagcaacgca ccggaataaa accgccaaag
    17221 ctgtcatgct caatctcgta gcggttaaat tccaccatca cgccgcgtgg ctctttcacg
    17281 gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag cgccatcatt
    17341 gtcggcagta aatcaatatg actgactggc gtatcgacct gtcgccgctc cccttgcggc
    17401 gaacggatga tcagcggaat acgggtgatg tcgtcataca tcgccgctcc tttactgatc
    17461 agcttatgtg cgcccatcat ttcaccgtga tcggaggtat aaatgaccca cgtattttca
    17521 cgttgctctg gcgttaaggc attgatgaca cgtccgattt ggtcatcaac aaagtcattg
    17581 caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga tggcatcgcc
    17641 tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctgtga tttctcgcca
    17701 agttcgtagt aaaaatcagc gtatttctct aaatactcca ccggacaggt gaacgggtga
    17761 tgcggctcat catacgaaac caccatcagg aagggttcct cggcgcgcac gggctattgc
    17821 agaaaatcta ccgctcgatt gctgatgcgg tgcgcccagg tgaaggtttc gtcaatatgg
    17881 ttcgcctgta aatcttcgac gctgtttagg ccattgcgcc acaggctgat ctctttttcc
    17941 gtcagttcgc taagatagtt cgccccatca aaccagtaat cagcatccca ctccggcgga
    18001 cactcgccag tgccgaaata gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg
    18061 tgatagccgg catccttaaa gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg
    18121 ttgttggtcc acgggccgga ctggttagcg tagataccgg tgaatagtcc ggcccgtgca
    18181 ggcgtacaaa ccggtgaaca agtgtaggcg gaattaaagc gaataccttc cgccgccaga
    18241 ctatcaatat tttgcgtatt cagcggttta ccgctatagc aaccgaccat attggtggcc
    18301 tgggtatcgg tcaattgggg cgtttcatag gctatccttc gcgtcgggag taaaggttac
    18361 ggctgggtcg tgacgatgcc accagctgtc aaaaatcagg tagacaatca ctacggcagc
    18421 gatgaaataa ctgatcatcg ctaaccagcg cgtaccgtag ccgccgaatt cagccagccc
    18481 gacatagacg ccaatcatcg cgaacaggat accaattgac gcgatcttga cgtttttcca
    18541 cggtttcatg tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgat
    18601 aaaaccgatc accagcatca cgaccacgtt gatgcagaac gtacaggcca gcacatagag
    18661 gaaatggaag tcgaacttca ccagatagtt gatggtgatg tagctgatta tgccaatccc
    18721 catcgccact tttgccgcca gcgccgggat gcgcggaaag aaaaagccca taatgatgat
    18781 ggtaaccagc ggcacgttgt aaatgccgtt gagctgtttc atccagctat acagcccctg
    18841 cggcgcgttg gcgatccacg gcgctaccaa aaccgaaacg atagcgataa agaaaccaaa
    18901 tttgcgcccg acggtgacca gctgctgcgg ctcggcattc tggttaatga tgcgacggta
    18961 aatacccata ctgaataatg tactggcgct attcagaaag ccgttgaagg tactgatcac
    19021 cgcaccaaat aacaccgcgc cgaagaaacc ctccagtggc actggcaaaa cgttattgac
    19081 cagcgtcggg taggccatgt cggctttcgg tagatcctga tacagatgaa acgcaatcaa
    19141 ccctggcagc accagtacca gcgggtcgag cattttcagc accgccgtta acagcgcccc
    19201 cttttgccct tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt
    19261 acaccagtaa aaggtgttca ccagaattaa accggtaaat gccgcgccaa tcggcaaggg
    19321 atcggtcggg ccaccgactg agtttaattt ctcggcgtga acggtggtaa tttgctcaat
    19381 gccctgcata aagctgccct tgcctatcgc gatcaggcca aataccggca ccatcaaccc
    19441 gccgataacc agcccaatac cgttgatgga gtccgccact gccattgcgc gcagtccgcc
    19501 gatcaccgca tacaaaattc ccgccagacc aagcaaaatt accagtagcc agatagccgc
    19561 accgtgagaa atctgtagcg attccccgac gtgaaacagg ctgttgagcg ccaacgcgcc
    19621 ggagtagaga acaatcggca gaaagcagac gccggtggca atgaggaagc agaagtcgat
    19681 gataatacgc gtcgttttat cataacgttc ctccagaaaa tcggggatgg tggcaatgcc
    19741 gcgcttcaga tagcgcggta aaaagattag cgcgaggaag atcagcgtca ccgctgaagt
    19801 cacttcccag cccatcaccg acatgccgct tttgtaggcc tgcccggaaa gaccaaccag
    19861 ttgttccgtg gaaaggttgg ttaacattaa cgaagcggca ataaccggcg cttttagtga
    19921 acgtccggca agaaaatagc cttgttgcga tccggtatct gttttgcgaa ccttccacca
    19981 ggtgatcacc gccaccagca gcgtaaaacc gacaaaactc aagatttgta acgaattcat
    20041 ctcatagccc ttatttatgt ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc
    20101 gtcgggtcag gcagtcagat tcttaaaata caaacgtcgt atccctgaac ggattgtttt
    20161 ctgttaagtt caggttgtga gcataattcg ccggagggat attaaaatga atggaaaatt
    20221 gcaaagttca gatgtaaaaa acgaaactcc gtacaatatt cccttattga ttaacgaaaa
    20281 tgtgatctcc agcggaattt ctctgatctc gctctggcat acctacgccg acgagcatta
    20341 ccgggtgatc tggccgcggg acaagaagaa accgcttatc gccaactcat gggttgcggt
    20401 gtataccgta caaggatgtg ggaaaattct tttaaagaat ggcgaacaaa taactctgca
    20461 tagcaactgt attatatttt taaagccaat ggatattcac tcttatcact gtgaaggttt
    20521 agtctgggaa cagtactgga tggaatttac ccccaccagt atgatggata ttcccgttgg
    20581 tcagcaaagc gttatttata atggcgaaat ttataatcag gaactcaccg aagttgctga
    20641 gttaataact tcaccagaag caataaaaaa taatctggca gtcgcttttc tgacgaaaat
    20701 tatttatcag tggatttgtc ttatgtccgc agatggtaaa aaagatccac aacggcggca
    20761 aattgaaaaa ttaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga
    20821 tatggctgcc acgatcccct gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac
    20881 cggcaagacg ccgaaagaat attacctcga tgcgcgtctg gatctggcgc tatcgctatt
    20941 aaagcaacaa ggaaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt
    21001 tcatttcagc aaagccttta aacataaatt tggttatgcg ccgtcagccg tgctaaagaa
    21061 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
    21121 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
    21181 tttcgacaac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
    21241 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
    21301 gccgaccacc agtggttcag gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc
    21361 ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat gttgaggatt
    21421 ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa ttgctgccgg gttggagtag
    21481 ttgagcatct aggcgtttgg tgaatatttt tccatataat ccaccagttc caggacgccg
    21541 ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg accaacaaca
    21601 ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg ggtacttgcc gacgcggatg
    21661 tgcgccatga caaaatcaac gtcgctgaac gccacttcag gatcggtggt gtaactaaac
    21721 gcaatgtccg gcgctttttc tttaaggatg actttacagg cttcggcaat cacttcctgc
    21781 cgcgcgccat cgttatcata aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc
    21841 aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac aaccactgag
    21901 aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga tgagaattaa
    21961 ttaagctatc gagctgttca cgcaattggg atacatgcag accaatgatc acctgaatgg
    22021 catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg
    22081 tttgtgacat gtcatgcagt gcaatacgta gacgcgtcgc gcaattgtta atgctggaga
    22141 tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc tctttttttg
    22201 gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag tagagtttca
    22261 cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg cagaatcagg gtgcggaaaa
    22321 ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc atcatatccg
    22381 cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc agacctctgc
    22441 ccatgttgcc caccacacca aagagataca ttacggtcga cattgaggcc gccagcacgg
    22501 cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc ggttcggtaa
    22561 ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact tttacccgat
    22621 tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca aagatttttg
    22681 agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagtggc tcggcgctca
    22741 gactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca acagctgccg
    22801 gaccaaagat aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt
    22861 cgaggaaggt gtaaacccac accccaagcg cacccgccga acgcaggaac gcttgcagag
    22921 attcaatccc catttgtact tttggccagc cgagcaatgt cagccaggca caggggatca
    22981 tcaccaggaa ggcgataatc accacataag acgtcccctg gaaaatgccg agaaaaaccg
    23041 gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa atgataattg
    23101 cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga ccgctacctg
    23161 ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt cccatcgcat
    23221 tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca cgtccctgcg
    23281 cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata ttacggaata
    23341 ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg ttcggatcgg
    23401 tcagtgattc cccgacaaaa catcgggttt tgcagcaaga tggcaagacc caccacaatc
    23461 ccggcgaagg gaaacagcag cactggcgtg aacatcgcgc cgccaaagcg ttgaatttga
    23521 ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga ttggttatgc
    23581 ggcagacctg gagcgaggat aggaagatgc gatcggcaga acatgacgac ccaatgggat
    23641 tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg cgaaaaagta
    23701 gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag atttttttca
    23761 atcgattaag cagaacttgt gggcgcatta cccgtgcttg caggcaaaaa agagagctag
    23821 agatgatcta caaaagcatt gcggagcggt taagaattcg acttaattcc gcggatttca
    23881 cgctaaacag ccttcttccc ggtgaaaaaa agctggcgga agagtttgcg gtatcgcgga
    23941 tgactatccg taaagccatt gacctgctgg tagcgtgggg gctggtggtc cgccgccacg
    24001 gcagcggcac ttacctggtg cgcaaagatg tgctgcatca aaccgccagc ctgaccggac
    24061 tggtggaggt gttaaaacgg cagggaaaaa cggtcaccag ccaggtgctg atttttgaaa
    24121 tcatgcctgc gcctccggcc attgccagcc agttacggat tcaaatcaac gagcagatct
    24181 acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct aatgctggaa gacagctata
    24241 tgccggtaaa actgttccgt aacctttcgc tgcaacatct ggaagggtcg aagtttgaat
    24301 atattgaaca agagtgcggg attttgattg gcggtaatta tgaaagcctg atgccggtgc
    24361 tcgccgatag actgctggcg cggcaaatga aggtagcgga acacacgcca ctgctgcgga
    24421 tcacctcgtt gtcatatagc gagagcgggg agtttttgaa ttattcagtg atgttcagaa
    24481 atgccagcga atactaggtg gagtaccatt tacggcgact ccacccggaa aaaagttaac
    24541 cgatactcca gaagagcacc gccagtaatt ggggggtgat aatgcgcagg aacatcacta
    24601 acggatagac agtggcgtaa gagagcgccg ccgcaccact ggttggatga agattattag
    24661 caaacgccag cgccggagga tcggtcatgg aacctgccag catcccgcac atggtcaggt
    24721 aattcatttt ggctaacatc cgcgccagaa tgccaacagt aatcagcgga acggcggtga
    24781 tcagggcacc ataaccaatc cagcttagcc cttcgccatt gaccagggta ttcacaaaat
    24841 ccccaccaga tttcagacca acgaccgaga ggaacagcac gatccccagc tcccgcagcg
    24901 cgaggttggc acttggcggc ataaaccagt acagcttgcc gatactgccg atacgcccga
    24961 ggatcaacgc cataatcagc ggaccgcccg ccagccccag tttcaacgcg gccgggaatc
    25021 ctggcacaaa gacgggaata gaacctaaca atacgccaag cccgatgcca ataaacactg
    25081 gcagcatctg aacctgttgc agtttttgct gcgcattccc cagcacattg gcaacggcat
    25141 caattgcgga cggacgcccc accaggttga ggatatcgcc gaactgcagg ctgatatcgc
    25201 cactggcgac cagttcgacc ccggcacggt tcaggcgcga gataacaacg tcatagcgtt
    25261 ctttaaagtg caggtcgcga atacgttttc cgagcacgtt ttcattggtg accaccacac
    25321 gctcgacgcg caaatcagtg cctttcgtag acagcgaggt atcgacctcc tgaccaatca
    25381 ccagttgcgc attatgtaaa tccgctggct gccccaccag atgcagcaaa tcgcccagtt
    25441 ggataatggt atctggcgaa ggtactttta gggtttcttc tcgtttcaga cgcgagcaga
    25501 taattttgtc gccgttgaga atcggtacat ctttaatggc taaatcatgc aggttagggt
    25561 tctcaacgcg aatattgata gtcttgatca acgcgccgcc attggtgcgt gaagactcgt
    25621 gctgctgagc ttctgtctcg acattgacgc ggaaaataac ccgcaacatc cacatggtga
    25681 acaaaatccc gcaaatgccg aatggatacg ccatcgcgta actcatcccc atctgatcga
    25741 ccatttccat tggtgtaccc aggtcgcgca agatctgctg ccctgccccc agcgctggcg
    25801 tattggttac cgcaccggag aaaatcccca gcactaccgg cagtggaata tcaaacagtt
    25861 tatgcaggat ggcggtaacc agaccaccga tgatgacgat cagaacagca aacaggttga
    25921 ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg ccctacctga atcccgatgg
    25981 tataaacgaa caggatcagg ccaaactcct gaataacatg cagcatatcg ctacttaatg
    26041 tcatccccgc ctgagaaaca aaatggccga cgatgatccc accaaacagc acgccgccaa
    26101 tacctaatcc tatgccgcga aatttgacgt tgccgataaa caaaccgacg actgccacca
    26161 aagccagaat actgaccgtt aatgctatat cactcatcgt ccatttctct tgagataaca
    26221 ttttaattgc tacggattat ggctgaatcg gtgaagcaga tttgccttat agcgcacatt
    26281 atcacgggaa agaacgtgcc gaaatatctt aaacagtcga aatttaacgc attgttgaag
    26341 aagaaagaca aaaaaagccc caccagaatg gcggggcaaa gagaatagct agttagctat
    26401 ttaacgcggg acgttcgctg atagcgatac gctgcgctgc gatgggttca ggctcattac
    26461 gaattaaatc aatatgcagt aaaccgttta cgaaggttgc gccagagact tccatatttt
    26521 cagccagcgt aaaactcagg ctaaatggct gattcataag cccttgatgc agccattttt
    26581 tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag gcgcgtacct tccagttgaa
    26641 tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt aatgcggtag tggttatcgt
    26701 cgcttttctc aatgttgtac ggcgggaagc tctggctttc accggcgttt tgcagtgcgt
    26761 tggccagttt gtcaaaaccg atccattgac gcatcagtgg ggataaatcg aagttacgca
    26821 tagtcatttc tccttctaag aagcgagtaa gtacctgcaa atccgaagat tcgcatatgc
    26881 tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc ggccttaggg aattagttga
    26941 tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg ttcgagatcg atatacagca
    27001 aaccatttac caggttagcc ccacgaacat gaatgttctc agctaactgg aatttgcgtt
    27061 caaagttgcg ttcagcgatg ccctggtaca gataggtgcg ctctttttgt tcgtcggcgt
    27121 gagcaccttt caccaccagc agattatcct gggcggtaat ttccagttcg ctctcagcaa
    27181 aaccagccac agcgatagca atgcggtaat ggttttcgtc taccagttca acgttatacg
    27241 gagggtagcc gccattactc tggctctggt tgttttctaa gtggttaaac aaacggtcaa
    27301 atccaatagc agaacggtaa agcggggata aatcaaagtt acgcataatc aatagctcct
    27361 gaaatcagcg agaatgtaag accttccaca atggacaggt caggtagcca gaacacccat
    27421 caggcgcgtc ctcatcgact acgatgtaaa atgggtctgg aaatgaactt ttcaagcctt
    27481 atcagcggac ttttttttgc agtttatggt ctattgcata gactgagggg gcagcacgaa
    27541 acgttaaagt gcgatagccg ccacagtgcg acgtaatgat gatgttattt tcagcaaggg
    27601 atcgctataa ctcatcaggc caacacccac gatcaacaga tgaatttatt atgataagaa
    27661 atgtgttgtt agcgttcatg atatgcagcg gaatgacatt actcggagga tgctccagcg
    27721 tgatgtccca taccgggggg aaagaaggaa cgtatcccgg cacccgcgcc agcgccacca
    27781 tgattggcga cgatgaaacc aactggggca ctaagtcgct ggcgattctc gatatgccgt
    27841 ttaccgctgt aatggacacg cttttactgc cgtgggatgt gttccgcaaa gacagctcgg
    27901 tgcgctcgcg cgttgaaaaa agcgaagcca acgcgcaagc caccaatgcg gttattccac
    27961 cggccagaat gcctgataac tgatttagcg atcagtttcg gtgatccaca gctggccacc
    28021 ttccatccac gccagccatt gaccatccgg cgagaagacc acggcgtccg cagaaggtgg
    28081 attggcgtgg ttttcggtta aatactcaac ctcgccgctt tgcgcatggg cacaaacaat
    28141 tcgattatcc agcacaaagc ccaaccattc tcctgacgga tgccagttaa atgcagactg
    28201 aatatccgtt ttgttatggg ttaacttgcg cggttcgccg ccctgtggcg agataagcca
    28261 cagttgcaca atgccgttat catcacgcat taaaaacgcg atttgcgtac cctgcggatt
    28321 acagcgcacc cagtggcgcg ggacgttgac taaccccgga taagcccgat gatgggtaaa
    28381 ggttaaacgt cgctgcacga cgccacgcgg tggcgcgggt agggttgttt ccgttccact
    28441 taacggcgca tcacctgccg atttccagct agcttcatct tgcggtaact caacgataaa
    28501 tagctccggc actttctcgc cctttggcga aagtgtgtcg ccaataaacg ccagcgcgtg
    28561 atttcctacc catccttctt cataagcacg attgatttca tcactgccag gctgcggcgt
    28621 gggcgtggtt ttactcacca gcacacacca gtggctaccg ctgtattcac gcggatgttg
    28681 cttttgtacg ttgaccgggc caaacggtgc agcaaccccg acgtttcgca aatccagcgc
    28741 cggatcgagt tgatgcatta catggtcgtt ataggtaaag ctcccccttt caccgttcgg
    28801 gctaaagaca tgcacatggc tgccaccgcg cagcgctcct ggcgtatacg gcgcggtaat
    28861 atccattgca tcgagattgc tcaccttgcc gccttcagca atgactccgc gtcgatgatg
    28921 gaagtcgtaa tgccatgttt catcaggatt ttcagggccg tgaataaaga catatttctc
    28981 tgacttcgga tgaacggtca ccacgccgac gtgtgcgccc tgtgacgcgc gatagataac
    29041 ctcgacctcg cctgtatgga tattcacacg ctcaatggtc tcgccggtaa acgacgcccc
    29101 agaaggacgc acgtcaaata ccagccactg gctgtcgggc gtccaggtat tggtattggt
    29161 attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc ggtcctgata
    29221 caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat agtcttttgc
    29281 catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta ttggcctcgg
    29341 cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga tcgcgctgga
    29401 taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc tgctggcacc
    29461 ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg tgatcgccaa
    29521 tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc gtaactacca
    29581 gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat cgctgatccc
    29641 cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg aggatgataa
    29701 atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc gctatctggt
    29761 cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc atcaaatccg
    29821 taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt tctactcctg
    29881 catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag atggttattt
    29941 tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga cgctgaaaga
    30001 gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga aatgcacggt
    30061 gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct ttctgattgg
    30121 tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg cgctggatag
    30181 cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg ccgcttactg
    30241 gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa gccgatgcct
    30301 gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata ttccacagtt
    30361 gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga gcgcgccaat
    30421 caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct gcgccaggta
    30481 ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa acacgccgcc
    30541 ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg taatcgaagc
    30601 aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg gatcgttggt
    30661 gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg gcgttttacg
    30721 cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc cagagagcag
    30781 gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt tcagcgccgt
    30841 gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc acagtgcaga
    30901 agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca ccagtttcca
    30961 gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac catccaccag
    31021 accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg tcaggcgcgg
    31081 cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac cagtgacaat
    31141 gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag tcaaaaacgc
    31201 cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt catgttccgg
    31261 gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga aaataccggt
    31321 tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga ataaagtcac
    31381 cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc gatctaaaaa
    31441 ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa atacatagcc
    31501 catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca cggccaagtt
    31561 agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg tcagataacg
    31621 ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc tccagatttt
    31681 gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat ttttgtattg
    31741 ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc tacgctgtta
    31801 tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa ctcaataact
    31861 ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa gaagccgcca
    31921 accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc tttgttgtaa
    31981 tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacaaagtc gatatgcagg
    32041 caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac atcataggct
    32101 tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga gagatccggt
    32161 tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg tgagaacatg
    32221 cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta gtattcggct
    32281 tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag ttctttaatc
    32341 agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa ctcaatctga
    32401 ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac cgcgcgggag
    32461 ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa gccgatttcg
    32521 cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac ccaactgtag
    32581 gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc attcagcact
    32641 ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat caggattgga
    32701 ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat gcgcgaagga
    32761 tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt acgggcgcgg
    32821 ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat tttcaggaac
    32881 atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc actgcctctc
    32941 gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac tgcccggcgc
    33001 gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac tgcgccaggt
    33061 tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat accgctttta
    33121 acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc gcctgcgcgc
    33181 ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa acggtcatgc
    33241 cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg atgagctgac
    33301 agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct gcgccaatca
    33361 acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat tgtggggaat
    33421 tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca tgcgccagcg
    33481 cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt tgccactgca
    33541 tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc cgccacgtcg
    33601 caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc ggcgacaagg
    33661 gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc gccaatcaac
    33721 aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac atgcgaggcg
    33781 cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc aaggccagcc
    33841 gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa tgagtgattt
    33901 aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat ttgctggcta
    33961 tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga aggagccaga
    34021 gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt atcgtcatga
    34081 gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa ttgttcgcca
    34141 atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc gacattgctg
    34201 ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt cgtgacttct
    34261 gctaacacca cagccggaga ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg
    34321 ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga tccccagtca
    34381 attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag ctggcaatca
    34441 tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca tcgaacagcg
    34501 ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag gttcgttcaa
    34561 aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc gggttatgca
    34621 ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct
    34681 gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg ctggaggtcg
    34741 cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact tcgctcatgg
    34801 cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg tcagtgtcga
    34861 ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc atttcaatca
    34921 gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag gttgcaaact
    34981 cttcacagag ctccgcctca gccggaagtg gcgagccagg tacgtatttg ccgtgaacga
    35041 tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg gagagtcctt
    35101 gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc aactttgtag
    35161 taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac ttatattgtt
    35221 atgctacaat ttagatcaaa aaaagaacaa tacataaaaa aatgacatgc gtcgggcaga
    35281 aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc tttcaacggc
    35341 ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt tttaaggctg
    35401 ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct ctggtttccc
    35461 ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc actgggctat
    35521 cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt
    35581 accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca gcacttaacc
    35641 ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat
    35701 ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag gcactggcaa
    35761 cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa aacctaaaag
    35821 gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg ttctaccatc
    35881 catttaaagt gcgattttat gaatactatt cacccagagg cattccggat ggtgtttccg
    35941 cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg caatttctgg
    36001 ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg
    36061 cttacagatt aattcagcac atacttctca atagcaaacg ccacgccatc ttcaaggttg
    36121 gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc catcgccaca
    36181 ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt
    36241 tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc tttgttaacg
    36301 cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata tttctctttc
    36361 acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc aatcatcatc
    36421 actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaatt
    36481 gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc ggtgtacagc
    36541 gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa
    36601 cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc ggccttctgt
    36661 accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc
    36721 aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt cacgccacgg
    36781 gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga
    36841 agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca aggtagatta
    36901 gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag
    36961 gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc cctttcagca
    37021 gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca ccttgcggtg
    37081 agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc gtactggtat
    37141 ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc gactccagtt
    37201 gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag agaatggccc
    37261 ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc tcctcaaccc
    37321 aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacttcat
    37381 caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatc
    37441 tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct ccgccctttt
    37501 cttatagctt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgcaaa
    37561 atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg cacgctcgca
    37621 tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt caataaacgc
    37681 acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag cggcaatcgc
    37741 atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg tttcccacag
    37801 ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc cacgacggga
    37861 ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac gacgctcgcc
    37921 acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat
    37981 acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag tatccacacc
    38041 gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg tgtggacatc
    38101 aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac
    38161 ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg gctgatagat
    38221 aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca ttttctgcgt
    38281 cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg gtgcactggc
    38341 gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat ccatcgcttc
    38401 gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg gcggctgagc
    38461 gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga aggtcaacag
    38521 cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata
    38581 acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg cggtgataag
    38641 cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga cgttaaggat
    38701 tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc
    38761 gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc gttcctggca
    38821 gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc gggtcatttc
    38881 acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc caaccacgat
    38941 tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca tctgctgttc
    39001 aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg agaatttcgg
    39061 gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc
    39121 gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca gggtacgggt
    39181 catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt
    39241 aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt caacgccaat
    39301 accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt tgttcttgtt
    39361 cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc
    39421 acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg
    39481 tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga ggctgggcca
    39541 gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg gagcctgcgg
    39601 tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa ccagctccag
    39661 tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt
    39721 ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc
    39781 cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat cctgtacaga
    39841 gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc
    39901 gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt
    39961 gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat
    40021 actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat
    40081 ctgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa
    40141 cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg
    40201 caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac
    40261 accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag gcgcacatca
    40321 acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc
    40381 gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat
    40441 tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg aaagagaaag
    40501 tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc gcgataccgg
    40561 cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt tctttatccc
    40621 acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc tgcttgagca
    40681 atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag catccccagt
    40741 cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta
    40801 tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc gtaccgtcaa
    40861 tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc tcgcgctctt
    40921 cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg acgcgaccaa
    40981 tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc agcacgctgg
    41041 ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa gcgagatccg
    41101 cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat
    41161 tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca cgctggaaac
    41221 cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa catccagcac
    41281 atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc
    41341 ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt cgctgacata
    41401 caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa acgcctgctt
    41461 gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa cgcgacggta
    41521 atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa cgtgggcacg
    41581 aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga gcatacgcat
    41641 cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag attgaccaat
    41701 tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca gttcttcacc
    41761 ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg ccatcgaaaa
    41821 ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct
    41881 ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg aaaaacggct
    41941 acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt ccgcgccttc
    42001 aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg ctcctggctc
    42061 gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag taccggtcag
    42121 cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg
    42181 accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt
    42241 aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct tctttgatat
    42301 cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt
    42361 cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc tctttcgcta
    42421 gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat
    42481 ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca
    42541 gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg
    42601 cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc agctcacgta
    42661 cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa cgaatgtcat
    42721 tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc ggttcgatcg
    42781 ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt
    42841 tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg
    42901 tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca
    42961 gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg
    43021 ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct
    43081 tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc agacccgtgc
    43141 cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc tggcaagccg
    43201 ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt
    43261 ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa cctgagcgcg
    43321 tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc
    43381 tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct
    43441 gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt
    43501 cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca
    43561 gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca
    43621 atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag
    43681 tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgct
    43741 tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg
    43801 atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt
    43861 ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct gccgcgaggc
    43921 gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat
    43981 cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt ataatcctcc
    44041 acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc tgcgaaaagg
    44101 tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca attattacaa
    44161 tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc
    44221 caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca
    44281 accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa
    44341 tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa
    44401 gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa
    44461 ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta
    44521 ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga
    44581 aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt gaaagcttcc
    44641 gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg
    44701 ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc
    44761 gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct
    44821 actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg
    44881 caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc
    44941 tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta
    45001 acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc
    45061 tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca
    45121 acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa
    45181 ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt
    45241 gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg
    45301 ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc
    45361 ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg
    45421 ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac
    45481 acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac
    45541 gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt gaagcaatcc
    45601 atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgt
    45661 ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga taccattgcc
    45721 gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct gcaacagtat
    45781 ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac cgctaatctg
    45841 ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca gcctggtcag
    45901 actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa aatggcagct
    45961 gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat ctctcagccg
    46021 ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc
    46081 atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc
    46141 atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat
    46201 gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg
    46261 ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg
    46321 ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg
    46381 gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag
    46441 aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac ctttatgccg
    46501 gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta ctatatcgtc
    46561 agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc
    46621 ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg
    46681 tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc cgccacacaa
    46741 agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac gcctccggga
    46801 cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt tgccgaaacc
    46861 gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa agacgccgac
    46921 ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc
    46981 gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct gctgttaaaa
    47041 cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg
    47101 tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct tatcgacgcc
    47161 agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg
    47221 gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat
    47281 ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca gcttaatgac
    47341 gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa
    47401 gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct gttaaacgcg
    47461 ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg tgacgtgctg
    47521 cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc cgggctacgt
    47581 gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga aattgaacag
    47641 gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga tccggcagag
    47701 atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt ggtacgcaat
    47761 aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca cgcgttaatt
    47821 cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct caaacagagc
    47881 atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca cttacaggcg
    47941 ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg agcctgggca
    48001 ggtgaactgc tagcggaaga gttgcgcctg gcacagcaga acttaagcga aatcaccggg
    48061 gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat tggtaagtaa
    48121 ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt gttagcactc
    48181 ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat aatctttaaa
    48241 aaaagcattt aatactgctc cccgaacgat tgtgattcga t
//
LOCUS       DAJTUN010000014        47372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTUN010000014 DAJTUN010000000
VERSION     DAJTUN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.014"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="OO470_000781"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="OO470_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6250125.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="OO470_000782"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="OO470_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6250126.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="OO470_000783"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="OO470_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6250127.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="OO470_000784"
     CDS             complement(2946..4187)
                     /gene="tolA"
                     /locus_tag="OO470_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6250128.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="OO470_000785"
     CDS             complement(4252..4680)
                     /gene="tolR"
                     /locus_tag="OO470_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6250129.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="OO470_000786"
     CDS             complement(4684..5376)
                     /gene="tolQ"
                     /locus_tag="OO470_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6250130.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="OO470_000787"
     CDS             complement(5373..5777)
                     /gene="ybgC"
                     /locus_tag="OO470_000787"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6250131.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="OO470_000788"
     CDS             complement(5927..6220)
                     /gene="ybgE"
                     /locus_tag="OO470_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6250132.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="OO470_000789"
     CDS             complement(6220..6333)
                     /gene="cydX"
                     /locus_tag="OO470_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6250133.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="OO470_000790"
     CDS             complement(6348..7487)
                     /gene="cydB"
                     /locus_tag="OO470_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6250134.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="OO470_000791"
     CDS             complement(7503..9071)
                     /gene="cydA"
                     /locus_tag="OO470_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6250135.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9920..12553)
                     /gene="mngB"
                     /locus_tag="OO470_000792"
                     /pseudo
     CDS             complement(9920..12553)
                     /gene="mngB"
                     /locus_tag="OO470_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(12571..14487)
                     /gene="mngA"
                     /locus_tag="OO470_000793"
     CDS             complement(12571..14487)
                     /gene="mngA"
                     /locus_tag="OO470_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6250136.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14657..15379
                     /gene="mngR"
                     /locus_tag="OO470_000794"
     CDS             14657..15379
                     /gene="mngR"
                     /locus_tag="OO470_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6250137.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15482..16351)
                     /gene="sucD"
                     /locus_tag="OO470_000795"
     CDS             complement(15482..16351)
                     /gene="sucD"
                     /locus_tag="OO470_000795"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6250138.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16351..17517)
                     /gene="sucC"
                     /locus_tag="OO470_000796"
     CDS             complement(16351..17517)
                     /gene="sucC"
                     /locus_tag="OO470_000796"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6250139.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17611..18828)
                     /gene="odhB"
                     /locus_tag="OO470_000797"
     CDS             complement(17611..18828)
                     /gene="odhB"
                     /locus_tag="OO470_000797"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6250140.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(18843..21644)
                     /gene="sucA"
                     /locus_tag="OO470_000798"
     CDS             complement(18843..21644)
                     /gene="sucA"
                     /locus_tag="OO470_000798"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6250141.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21945..22661)
                     /gene="sdhB"
                     /locus_tag="OO470_000799"
     CDS             complement(21945..22661)
                     /gene="sdhB"
                     /locus_tag="OO470_000799"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6250142.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22677..24443)
                     /gene="sdhA"
                     /locus_tag="OO470_000800"
     CDS             complement(22677..24443)
                     /gene="sdhA"
                     /locus_tag="OO470_000800"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6250143.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24443..24790)
                     /gene="sdhD"
                     /locus_tag="OO470_000801"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24443..24790)
                     /gene="sdhD"
                     /locus_tag="OO470_000801"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6250144.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24784..25188)
                     /gene="sdhC"
                     /locus_tag="OO470_000802"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24784..25188)
                     /gene="sdhC"
                     /locus_tag="OO470_000802"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6250145.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25569..25676
                     /locus_tag="OO470_000803"
     CDS             25569..25676
                     /locus_tag="OO470_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250146.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25882..27165
                     /gene="gltA"
                     /locus_tag="OO470_000804"
     CDS             25882..27165
                     /gene="gltA"
                     /locus_tag="OO470_000804"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6250147.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27556..28122
                     /locus_tag="OO470_000805"
     CDS             27556..28122
                     /locus_tag="OO470_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6250148.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28173..30632
                     /locus_tag="OO470_000806"
     CDS             28173..30632
                     /locus_tag="OO470_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6250149.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30647..31378
                     /locus_tag="OO470_000807"
     CDS             30647..31378
                     /locus_tag="OO470_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6250150.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31375..32451
                     /locus_tag="OO470_000808"
                     /pseudo
     CDS             31375..32451
                     /locus_tag="OO470_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32603..33649
                     /locus_tag="OO470_000809"
     CDS             32603..33649
                     /locus_tag="OO470_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6250151.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33646..34437)
                     /gene="nei"
                     /locus_tag="OO470_000810"
     CDS             complement(33646..34437)
                     /gene="nei"
                     /locus_tag="OO470_000810"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6250152.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34473..35207)
                     /gene="pxpA"
                     /locus_tag="OO470_000811"
     CDS             complement(34473..35207)
                     /gene="pxpA"
                     /locus_tag="OO470_000811"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6250153.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35197..36129)
                     /gene="pxpC"
                     /locus_tag="OO470_000812"
                     /pseudo
     CDS             complement(35197..36129)
                     /gene="pxpC"
                     /locus_tag="OO470_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36123..36779)
                     /gene="pxpB"
                     /locus_tag="OO470_000813"
     CDS             complement(36123..36779)
                     /gene="pxpB"
                     /locus_tag="OO470_000813"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6250154.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36802..37545)
                     /gene="ybgI"
                     /locus_tag="OO470_000814"
     CDS             complement(36802..37545)
                     /gene="ybgI"
                     /locus_tag="OO470_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6250155.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37816..39297
                     /gene="dtpD"
                     /locus_tag="OO470_000815"
     CDS             37816..39297
                     /gene="dtpD"
                     /locus_tag="OO470_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6250156.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39447..40865)
                     /gene="phrB"
                     /locus_tag="OO470_000816"
     CDS             complement(39447..40865)
                     /gene="phrB"
                     /locus_tag="OO470_000816"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6250157.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(40862..41371)
                     /locus_tag="OO470_000817"
     CDS             complement(40862..41371)
                     /locus_tag="OO470_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6250158.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41500..41787
                     /locus_tag="OO470_000818"
     CDS             41500..41787
                     /locus_tag="OO470_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6250159.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(41842..42222)
                     /locus_tag="OO470_000819"
     CDS             complement(41842..42222)
                     /locus_tag="OO470_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250160.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42225..46889)
                     /locus_tag="OO470_000820"
                     /pseudo
     CDS             complement(42225..46889)
                     /locus_tag="OO470_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(46908..47111)
                     /locus_tag="OO470_000821"
                     /pseudo
     CDS             complement(46908..47111)
                     /locus_tag="OO470_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11248 a        13006 c        11504 g        11614 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgtgttat gcattacatc gcgttcagac
      181 gtttttgtgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      301 catcagcagc ctttggtgac ttcggatagt ttttcactac cgaagcaaaa tagtacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcgccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 tcgcaccgct ttgatcgctg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accacgcagg gaatcaatat cggattgatt atcagaaagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcattag aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gaagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1081 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcatgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccatt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgcgg acgaccggcc tggtcagaag taaatgccag gttctggcta
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc agactaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc
     2101 tgacgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggctgcggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc acctttaatg
     2341 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agaaccatcc ggattcggag tgacctgacc gacaactaca
     2521 gcgtcaatgc ccagtgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     2581 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtccgaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgccaacg cagcctgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac ataccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggcag gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc cgctgccgct tttttgtctg cggcggcttt ttctgccgct
     3421 gctttcttat cagctgctgc cttatcagca gccgcttttt ctgctgcttt tttgtcggct
     3481 gcagctttct ctgctgccgc tttcttatca gctgcagcct tttcagcagc cgctttcttc
     3541 tcagcttctg ccttggcttt ttcagcagct tcagttgctg ctttctttct tgcttcagct
     3601 gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg cagcggctac ctcggctttt
     3661 ttctgcgctt cggctgcggc tttggcggct tctgcttctg cttttttctt cgcgtctgca
     3721 gccgctttct tcgctgcttc ttccgcagct ttagcatctg ccgccgcttt cgctgccgcc
     3781 acttcagctt gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc
     3841 tgctcctgag ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct
     3901 tgtttctcac gcagttcttc agcagcctgc tgttccttca tcttgcgctg ttcatcagaa
     3961 cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa
     4021 tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc
     4081 tcatcgaacg aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata
     4141 attatcgccc gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa
     4201 aaactgttcg cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg
     4261 ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat
     4321 tatttcatcg taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc
     4381 cttgaaacgg ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc
     4441 tttctcaacc accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt
     4501 atcgttactg ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg
     4561 ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa
     4621 cggaacaatg ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat
     4681 ggcttacccc ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa
     4741 ctcttccata aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgagacggtt
     4801 ataagccatt accgccggaa ttgcggcgaa cagaccaatt gccgtcgcaa tcaacgcttc
     4861 tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa
     4921 ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac
     4981 cgtaccgagg aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg
     5041 catagcacgc gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag
     5101 gcgcacaaac tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca gattatcccg
     5161 tttcccctga ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc
     5221 aaacgcttcg gcttcacgcg ccgctgcgtt aagaatacgg gttcgctgga taataatggc
     5281 ccaggatgcg attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc
     5341 cttcaggaac aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac
     5401 ttgggaagcg cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct
     5461 tcattcagca aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca
     5521 cgcattgatg ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa
     5581 tattccaccg tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga
     5641 ctgaagtgat gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg
     5701 gcgtggtaca ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga
     5761 aacagcgttg tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc
     5821 tgctactata cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg cgcgtaaaaa
     5881 tttatgggcc tggagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga
     5941 aaatcagccc gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa
     6001 gaaccttctg cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca
     6061 gcaataaacc atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc
     6121 aaaacataca tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct
     6181 tgtccattac cgcatacaag gtcgcgataa tcttactcat cagatgtctt cttgaccggc
     6241 tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag
     6301 aagcgttccc agaatccatg cgaaatacca catttttagc tccttactta gtacagagag
     6361 tgggtgttac gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag
     6421 gcggtgtaga gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc
     6481 gtcagctggc tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac
     6541 ggcatcacaa acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg
     6601 gaggagaaca caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc
     6661 agcagaacca cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc
     6721 agccatgcgc cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg
     6781 tccattgtcg atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt
     6841 gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc
     6901 agttcgccca cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg
     6961 ctcaccacgc ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag
     7021 tacagacgca gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct
     7081 acaccaatta ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta
     7141 cgccagcggg tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa
     7201 gacgccagca cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc
     7261 ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag
     7321 tgtggtgcaa tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg
     7381 gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg
     7441 ccaaccagca gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg
     7501 tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc
     7561 aggctgcttg ggccgaggcg tgcaaacttg aacattaaga acaattctgc caccaggaac
     7621 agggtataca ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac
     7681 gaattcgcca cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca
     7741 gccacgaacc agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg
     7801 cgcagaagcc attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg
     7861 atgattgcca gcagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc
     7921 gctacacgcg ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc
     7981 acgtttggcg tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg
     8041 aactggtcac gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac
     8101 gccttcatcc cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata
     8161 accggggtat ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca
     8221 aatttgttcg tctcctcttc ctgatcagga atgccgaaca aagtaaaggc agcaggcgca
     8281 ggttgcgttt cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt
     8341 tcgtagccgg attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg
     8401 gcagcgatag caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat
     8461 gcgctgatac cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg
     8521 aatttcacct gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc
     8581 atagtttcaa agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaaccaga
     8641 atccacagtg cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga
     8701 actttaccca gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag
     8761 aaggccatca gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa
     8821 tagtaagacc agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga
     8881 gcgaagttga taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg
     8941 ccggagagga cgtagaccgt ttccataatg gccaggagga acgccatacc gagcgtcagt
     9001 ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact
     9061 atatctaaca tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag
     9121 aaagggtcac acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga
     9181 atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat
     9241 cccacgtata tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt
     9301 tgcatttttc gacggtgatc aatttatagc taaattgccg cctttcagcc aatttgatcg
     9361 agaacaattt atctcttttt gatgcccatt tccaagactt atacattgat aaatatcaat
     9421 ttctacaaat tatgttaatc atgtttcaac aaggtaactt tagtgatatt tatgtttaaa
     9481 atgtgtgaat tataagactg aaattagttt cggttggcaa ttaaagataa aaagagacga
     9541 agcaaaaaca gcacggttga ccttaattct ttttttggtc actggctaat acgacgcaga
     9601 gtttcaggat tattaataaa ggcataaatc tcccccccgc cgatattaac atttaattaa
     9661 cttataatta tattataatt aactcacccg tggttattat tttgttaaaa ataaagccac
     9721 atatacgtag cttgagagtg aagataaaca caaagttacc tgatgcgcta cgcttgccag
     9781 tgtctacatc atctctgcaa tatattgaat ttgcctgctt ttgtagaccg aataaagcgt
     9841 tcatgccgta tccggcatga acaaagcgcc ctttgtcaac aatctggccg cgcatatgtg
     9901 gcttcggttt gattgctatt caggcaagcc ggtaactgaa cgtccgtgac tggccgggta
     9961 aaaaaggccc cgatagattt gaaccttgat tttcctcggt ggtaatgtgt tcatccatca
    10021 tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca caggttgctg
    10081 attcagccgg attaaacagc cgtaaaatca cttcttgtcg gtcttcagct ttcttaagtg
    10141 cgcttatcag gcatcccact gggggcattt tcaacaaact ataactttcc ggcacgttga
    10201 atccggcttt gttgagcttc atcgcatccc atgggatttt gttgtaacac tgtactggag
    10261 tcagccatgc tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg gtataactca
    10321 ataaacttag gcgacaagaa agcagaccac gtagttgtga gtccgggact ggcattttaa
    10381 ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt ccccagtaag cccacgccgc
    10441 gcagcaacgt aatggcaaag gttttcttct cttcaccgat gacttcaaat tcacgtagcc
    10501 cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa ggctgcatag ttgagcatat
    10561 tccataccgg aaccggcgct tctttccagc cttcttgctg ccagttgttc attgcactgt
    10621 cgttcacggg gcgcgttagc gaaccaaact gggtatctgc cagaacacta tcggtgttaa
    10681 aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc ctggttatca aggttgatat
    10741 ccacatcaat gcgcctgttg ttatgactaa gagtgacaac caactcgaca tctactctgc
    10801 cagtggattg tcttgcgctg cgttgttgca gattacgcgg caccgccatt tcatagcgga
    10861 taacagccct gctctgccag gcctcatgaa taatctcgca ttgcggtttc gcattcgccg
    10921 aagtaattac ccactcttct tttgcgggtg aatagtcata ttcatcacca tcatcagagc
    10981 tttcttcaat ttgcaacacc cggtcatagc gcacaccgct atctttatct accagttgta
    11041 gcgttccatc ctcattgagc gcaatttgcc agaaagcatt ttccagtatc ccttcagcgt
    11101 cactttttgc cgcaatcacg ttgccaggct gattcgcttc gatataaagc gtgcgatagc
    11161 ccatagaagg gacaatctgg ttgatctgta tatcaaactc cataaaggga tcgtaattac
    11221 cgtaatgaac tatttgccga tcgattaggc ctggatcgat ctcacgggca tggcgaataa
    11281 aatacggtac aggctgaccg cgatcgtctc gtagattaaa ctggctggcg cgcagccgca
    11341 cagtggtgtt gatcacttct tcccgcggcc agggcatcag gttaaacagg acgagtttgt
    11401 cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat atagaaacgc agcagattat
    11461 ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc gcgatgaact ttgtcactac
    11521 agcagcagcc gatgctgtcg tgggcatgat tttttaagat ctctttccac attttctcca
    11581 gcaagccgtg gtggtatcca aaacccaacg tcctggccag cgttgccagc ggttccagca
    11641 gattaacaat cttattttca atacgtgcgt gggcaacttt gatatccatg cgcgtggaac
    11701 cgatggtgcg atgcacgcgc atatatttgc catcaataaa ttcccctttc agggttgcca
    11761 gattatctcg ctgcgcttcg atcttctcaa atacctcttc aaagcggctc atcacaaact
    11821 ttcgttgagg ataaatttcg cgcagtttct ccatcacttc gaagatattt tgctgcaatg
    11881 gcatctgatc atgcccattc ggcagcaaaa tctctttggt tacagacgct ttttccagca
    11941 cgtcaaaata actgtcgagg cgtttgcgta atccgttttc gtccgcaggt aagtacttac
    12001 cgatggcata acccaacggc atcacctgcg ccgtcacttc gctaccgtca ctgctttgcc
    12061 acaaaaactc ggttttatcc gtaccgtggc gctcagaaca tccacgccag aacatggtgc
    12121 aggtaatgcc aaatccattg tagatatgcg gcagttgccc ggacatgcca aaggaatcgg
    12181 gtaaataacc tattttcatc ggctcgccaa acgcgaggca gtcacgcatt ccgtacatca
    12241 gattacggac gatggattcc gcagaaacaa tcgtggtatc ggtctgggta tactaggggc
    12301 cgataatcaa cttgccggct tctaccagtt tcttcacgcg gtctttgttt tccggtttca
    12361 ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag tacgtaatat ttgtattcgt
    12421 tgtcctgttc cagtcggcac aggatctctt ccatattatt gaccagcaga atgcgtgact
    12481 cttcggtggt gaaataccac tcccgatccc agtgcatatg cggggtgatg tgaacgcgag
    12541 ataccgcttt catcgtcgtt tcctttttgt ttatggcatt acgccatcag tcagatagtt
    12601 gccatgctta accgcgtgac gccgccagat caggagaatt gcagtcgaga ttgcagcccc
    12661 caccaatgcc gcaccaaacc agccaattgc cgccataaca ccgcccgcgc cattatcatg
    12721 aagtaaaaag agcgagaaaa tgccagcacc gggtgtcgaa aggccgatat tcatcgcacc
    12781 gacaatagcg cccgttacca tagagcccag cacaaacgaa ccaataaccc gcagcggatc
    12841 ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc agccctaaca gccaggtgga
    12901 tttcccggtc tcaatttcaa actctttaaa caggcgcggt gcgagcatcg tggaagcggt
    12961 tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg gcatacgggc cgtaaacgcc
    13021 gttcgccatt gcgcccaggc agaatgcata agcggcttta ttcactggcc ctccaaggtc
    13081 aaaggaacac ataaaaccga gaatggcacc cagcaacagc gcgttacttc ctgacagacc
    13141 gttcagccag gcggtaagcg agttattgat ccaggcgaca ggttccccca ccacaaacag
    13201 catcagactg cccgctccca atgtaccgag caccgggtag agataaaaag tcaggaagcc
    13261 attgaattta ctgctaagac gcaagtgatt tttcatccag cgtatcaagt aaccggctat
    13321 caatccgcca acgaccgcgc cgagaaaccc ggagccgatc atgttggcgg caagtccagc
    13381 cgcaaagcct ggcgctaacg ccggtttatc tgccagcgaa taggcggtat aggctgccag
    13441 caccggtacc atcaaaattc cgagcagccc gccgcccagt ttgcggtaca tccacagcca
    13501 ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta ataccgcgac
    13561 cgccagcacc gtcccccccg cgacaatcaa cggtacggca aaagagatcc cgctcaacag
    13621 tgcctgtttc agctccgttt tgacgctttt caccggttgc gtatcttgtt gtaaggtacg
    13681 cgtctcatcg ccacgctcga gggtaagcga ttgttggatc aacgcttctg catggcgaat
    13741 cggctcggca acaggcacag aaagcgcagg aatgccgtta aaacgctcac tctccttgat
    13801 ggcgacttca gccgcaaaaa tacaggcagt tgcactattg agttgatccg ccgttaaacg
    13861 cccttcaata ccgttagcac cttgcttttc aacgtagaca ttcacgccga gtttgcgtcc
    13921 ggctttttcc agatactccg cagccatgta ggtgtgagca atacccgccg ggcaggccgt
    13981 tacgcaaacg atagttggcg cgttggaaaa agaggcagaa ggttgcgtgc ctcccttgtc
    14041 atccagcgcc gagagcaatt catcaggcgt cgtcgccgac tgtatacgcg cccgaatctc
    14101 atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc atgtgcgtag tacccgcttc
    14161 attgagggga atcgccagca acaccactaa atcaaccgct tccgggccat ccacgccttc
    14221 ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc gcttctttta ccgcagcagt
    14281 tttgccatgc ggcacagcca acccttcact taaggcggtc gggccaaggc tttcacggcg
    14341 atacacttct ttcagaaatt gctcagtact ggaaattttc cccagagcag caagacgttg
    14401 agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc gcattcagac acaacgcatc
    14461 gcggtgggtc agagtcgtca ggttcatcat gatcctcact tgatcgtttt tatagtcgcg
    14521 ccagtgtgcc cgacaaaaca ataccattgc gtaatcaatt tcacttgatt gaaattattt
    14581 gcattttatt tgtattgttt acaggatgaa tttatcgggc ataatggcta aattcgtatt
    14641 aatgagatgc taaataatgg gacacaaacc cttataccga cagattgctg atcgcattcg
    14701 tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
    14761 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
    14821 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
    14881 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
    14941 acacagtgaa gttctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
    15001 acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc gtgcgctatc gcaagcaaaa
    15061 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
    15121 ggtcatggaa aattcgaaat atcactttat tgaagaagtg aagaagatgg ttatcgatcg
    15181 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
    15241 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
    15301 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
    15361 gcgaaaatca tcgcgataag cacaaaaagg ccatcatacg atggccaacc atgtcgaaac
    15421 cgacagccct tcgccattcc tgtagaggga agcggcgagg gctatttctt attacagata
    15481 tttatttcag aacagttttc agtgcttcac cgatatccgc caggctgcga acggttttca
    15541 cgcctgcggc ttccagagca gcgaatttct catccgcagt ccctttccca ccggcaatga
    15601 tcgcacccgc gtggcccata cgtttgcctt tcggcgcagt cacaccagcg atgtaaccca
    15661 caactggctt ggtaacatgc tctttgatgt acgctgccgc ttcttcttca gcgctaccgc
    15721 cgatctcacc gatcatcacg atcgcttcgg tctgcggatc tttttcgaac atttcgagaa
    15781 tgtcgataaa gttagagcca gggatcgggt caccgccgat accgacacag gtcgactgac
    15841 cgaaaccgta atccgtggtc tgtttaaccg cttcataggt cagtgtaccg gaacgggaaa
    15901 cgatacccac tttacccggt ttgtgaatgt gacccggctg gataccaatt ttgcattcac
    15961 ccggggtgat aacgcctggg cagttcgggc cgatcatacg aacgcctgct tcgtccagtt
    16021 tcactttcac ggtcagcata tccagcgtcg ggatgccttc agtgatggtg ataatcagtt
    16081 tgatgcctgc atcgatagct tccagaatgg agtctttgca gaacggtgct ggtacgtaaa
    16141 taacagacgc ggaagcgcca gtagcagcaa cagcttcacg cacggtgttg aacaccggca
    16201 ggccgaggtg ggtagtgccg cctttacctg gagttacgcc accaaccatt ttagtgccgt
    16261 atgcaatggc ctgttctgag tggaaagtcc cctggctacc ggtaaagccc tggcagataa
    16321 ccttggtgtt tttatcaatt aaaatggaca ttatttcccc tccactgcgg caacaacctg
    16381 ctgagctgca tccgtcagac cttttgctgc aataatattc aggccgctgt cagccagttt
    16441 cttcgcgccg agttcggcgt tgttaccttc cagacgtacc acgaccggta cgttaacacc
    16501 cacttccgct accgcgccga tgataccgtc agcgatcagg tcgcaacgaa cgataccgcc
    16561 gaagatgtta accagaacgg ctttcacttt gtcgtcagag aggatgattt tgaacgcttc
    16621 ggttacacgt tctttggttg cgccgccgcc aacgtccagg aagttagccg gttcgccgcc
    16681 gtgcagttta actatgtcca tcgtacccat cgccagacct gcgccgttaa ccatacaacc
    16741 gatgttaccg tccagcgcaa cgtagttcag ttcccactgt gcagcctgtg cttcacgcgg
    16801 atcttcctgc gactggtcac gcatttcgcg cagatcaggc tggcggaaca gggcgttgcc
    16861 atcagcgccc agtttgccgt cgaggcaaat cagatcgccc tgtttggtga tgaccagcgg
    16921 gttgatttcg atcagcgcca ggtcgcgctc caggaaaatg gtcgccaggc ccatgaagat
    16981 tttggtgaac tgctgaacca gtttaccttc cagacccagt ttgaacgcca gctcgcgtcc
    17041 ctgatacggc atcgggccag tcagcggatc aagcgcaact ttatggatca ggtgcggagt
    17101 ttcttccgcc actttttcga tttccacgcc gccttcggtg gaggccataa agaccacgcg
    17161 acgggaacta cggtctacaa cggcaccgag atacagctct ttagcgatat cggtcgccgc
    17221 ttcaaccaga atctggttaa ccggttggcc attggcatct gtttgatacg ttaccagacg
    17281 cttgcccagc cagttttctg caaaagcacg gatgtcttct ttgctgttta caactttcac
    17341 accgcccgct ttaccgcggc caccagcgtg aacctgacat ttcactaccc acggaccggc
    17401 accgattttt gaagcggctt cttctgcttc gcgcggagta gtacaggcat aacccaccgg
    17461 tgctggtaag ccatagcggg caaacagttg ttttgcctga tattcatgta agttcatgtg
    17521 ttctgtccat ccttcagtaa tcgttatctt ttaaaccgta gaccgacacc ctatccacgt
    17581 cggtctacag tgcaggtgaa gcttaaacta ctacacgtcc agcagcagac gcgtcggctc
    17641 ttccagtaac tctttgatcg ttaccaggaa gcccacggat tcgcgaccat cgatcagacg
    17701 gtgatcgtag gacagcgcca ggtacatcat cggcaggatc tcaacctgac cattcaccgc
    17761 catcggacga tctttgatag cgtgcatacc cagaattgcg ctctgcggcg ggttgatgat
    17821 cggcgtagac atcagggaac cgaacacacc accgttggtg atggtgaagt taccaccggt
    17881 cagatcttca accgtcagct tgccatcacg gcctttgact gccagctctt tgattttctt
    17941 ctcgatgtct gccatgccga gggtatcgac atcacgcaga accggcgtca ccaggccgcg
    18001 cggcgtagaa accgccatgc tgacgtcgaa atagttgtgg taaaccacgt catcgccgtc
    18061 gatagacgcg ttcacttccg ggtaacgttt cagggcttca accaccgctt tcacgtagaa
    18121 ggacataaag cccagacgga tgccgtggcg tttttcaaac gcttcaccgt actgcttacg
    18181 cagatccata atcggcttca tgttgacttc gttgaacgtg gtcagcatgg cggtggagtt
    18241 tttcgcttcc agcagacgct ctgccacacg cttacgcagg cgagtcatcg ggacacgttt
    18301 ttcactacgt gcagccagag ccggttgcgc cgccggagca gccgctgccg gagcagactc
    18361 tttcgccggg gctttcgcca gatgtttttc cacatcttca cgagtcagac gaccacccac
    18421 accggtgcct ttaatggcgc tggcgtcgag attgtgttca gccagcaggc gacggatcgc
    18481 cgggcttaac gcatcgttgt tttgctcttc cagagacgcc tgctggcgtt gcgccggagt
    18541 ggacgctttc tcttcagatt tggcgctggt ttctttaccg gcgctgttgc cttcacgcag
    18601 gcgaccaagg atctgacgag acgttaccgt tgtaccttca tcttccagaa ccgcatccag
    18661 aatgccgtct gctgatgccg gtacttccag taccactttg tcagtttcga tttctaccag
    18721 cacttcatca cgtacgactg cgtcgccggg ttttttatgc caggttgcga cggtggcatc
    18781 ggctacggat tcaggcaggt cagggaccag aatatctacg ctactcattg tgtatccttt
    18841 atttattcga cgttcagcgc gtcattaacc agatcttgtt gctgtttctg gtgaacggac
    18901 atatacccta ccgccggaga ggcggaggcc gggcggcctg cataacgcag agaagcccca
    18961 aacggaatca cttcacggaa atgatgctgg ctgcagtacc atgcgccctg gttgagcggc
    19021 tcttcctggc accagacaaa atccttgacg tgagcaaact gctgcaacac ttcctgcatc
    19081 gctttatgcg ggaacgggta gagttgctcg atacgcacaa tggcgacatc gtgttgattg
    19141 ttcttacgac gctgttccag caggtcgtaa taaaccttac cagaacacat cactacgcgc
    19201 ttcacgccct tcggatcaag ctcgtcgatt tcaccgatgg ctggcaggaa ggtgccgttc
    19261 gccagttctt cgaggctgga aaccgccagc ggatgacgca gcagggattt cggcgacatc
    19321 acgaccagcg gacgacgcat cccgcgcagc gcctgacgac gcagcatgtg gtaaacctgt
    19381 gccggggtag acggtacgca aacctgcatg ttttgctcag cacaaagttg cagataacgt
    19441 tccagacgcg cggaggagtg ctccggcccc tgcccttcgt aaccgtgcgg cagcaacatc
    19501 accagaccac acatccggcc ccatttctgc tcgccagagg agatgaactg gtcgataacc
    19561 acctgcgcac cgttggcgaa gtcaccgaac tgcgcttccc agatggtcag ggtgcgtggt
    19621 tctgcggtgg cataaccata ttcaaacgcc agcacggctt cttctgacag tacggagtcc
    19681 cagacacgga acgcgccctg accgttatgg atgtgttgca gcggcgtgta agtggaaccg
    19741 ttagactggt tgtggatcac cgcgtggcgg tggaagaagg taccgcgacc ggagtcttca
    19801 cccgacaggc gaaccggaat gccttcatca accagcgtgg cgtaagcgag gttttccgca
    19861 ccgccccagt cgaacagttt ctcaccggct gccatcgcct ggcgatcgcc ataaatcttg
    19921 gcaacgcgag actgcatttc aactgcttct ggtaccgtac tgatacgttt agccagttcc
    19981 tgcaggcgct tcatctcaac tttgttcggg tactcttcgt cccattcgtg gttgaggtac
    20041 ggcgaccagg tgaaagagtg catgttcatc ggacgccact ctgccactac gcaatcgcca
    20101 gcatccagcg catcgcggta caggttaacc atctcggtgg catcttccag cgtcgccact
    20161 ttttcctgct ccagcttgtc agcgtagatt ttgcgcggtg tcggatgttt tttgattttc
    20221 tgatacatca gtggctgggt tgcgctcggc tcgtcggctt cgttgtggcc gtgacggcgg
    20281 tagcatacca ggtcgatgaa gacatcacgt ttaaaggtgt tacggaaatc gagcgccaga
    20341 cgggtcacaa aggcaacggc ttccggatca tccgcgttaa cgtggaaaat cggtgcctga
    20401 accatcttac cgatatcagt acagtacggt gtagaacggg catccagcgg gttagaggtg
    20461 gtgaaaccaa cctggttgtt gataacgata cgtaccgtac cgccaacttc ataaccacgc
    20521 gctttcgaca tgttcagagt ttcctgaacc acgccctgcc cggtcactgc ggcgtcaccg
    20581 tggatggtga ttggcagcac tttgttgctg ttcggctcat caagtctgtc cagacgggca
    20641 cgaacagagc cgataacgac cgggcttaca atctcaaggt gagacgggtt aaacgccagc
    20701 gccaggtgca ccaggccgcc atcggtctgg aagtcagacg agaagcccat gtggtatttc
    20761 acgtcgcctg tgccgaggtg ttctttatgt ttaccggcga actcgtcgaa caagtcttgc
    20821 ggttttttac ccagcacgtt caccagcacg ttcagacgac cacggtgcgc catcccgaga
    20881 accacttcgc gggtgccgct gttgccagcg tggcggatca tctctttaag catcgggatt
    20941 aacgcgtcac cgccttccag cgagaagcgt tttgcgccag ggaattttgc gccgaggtaa
    21001 cgctcaaggc cttcagcggc ggtcagttcg cttaagaagc gttttttctc ttcgctattg
    21061 aaagtcgcgc gaccagactc gatacgctgt tggatccagc gtttttcttc ggtgctggtg
    21121 atgtgcatat actcggcacc aatcgggccg cagtaggttt gcttgagggc ggaaataagc
    21181 tcgccgagtt tcatggtttc tttgccgctg gcaaatgaac cgacgttgaa ggtttcctgg
    21241 aagtctgctt cggtcagatc gtggaaagac ggatccagat cggccacttt atcttgctgc
    21301 cacagtccca gcggatctag attcgcatgc tggtgaccac ggaagcggta tgcgttaatg
    21361 agctgcagga ctttaacctg cttcacattg gtgtcagggt cggagatcgt tgaagagtaa
    21421 cgtgaagcgt ctttcgccag gcggcggaaa tattcacgcg tttgagagtg gaattgatcc
    21481 ggtttgactc ccgtaccagg taactgctgg aacgtcgaac gccagttagc gtcaaccgag
    21541 tcaggatcgg ttaagaagtc ttcatagagc tgttctatcc agctctggtt tgcgccagag
    21601 aggtaagaag agtccaacca ggctttcaaa gcgctgttct gcatcgtgat cccttaagca
    21661 tcttttttat gcttacttcg ccgtggatac taccacgcac agtgatgtgc gtgccggggt
    21721 tcacttgcga gctcttgtct acgtagtggc tcgcgaagga acctttaaaa actgtcttag
    21781 tgtcaggggc agtttttaaa gatttcctgc atttctttgt tacgcctgat gcgctgcgct
    21841 tatcaggcct gcgggtaatg acttgtaggc cggataaggc gttcacgccg catccggcac
    21901 tggttgcctg atgcaacgct tgcgcgtctt atcaggccta cagtttacgc attacgttgc
    21961 aacaacatcg acttgatatg gccgatggcg cgcgtcgggt tcagcccctt cggacataca
    22021 ctgacgcagt tcatgatgct gtgacagcgg aatacgctga atgcatcgct caaaccgtcg
    22081 aggcggctgt cagtctcggt atcacggcta tcgatcagga aacgatatgc cgctaacaag
    22141 cctgccgggc cgataaactt atcgggattc caccagaaag acggacaaga ggttgaacaa
    22201 catgcgcaga gaatacattc atacagcccg tcgagttttt cgcgctgctc tggcatctgt
    22261 aaatgctcgc gagctggcgg attttgtcca ttattcaaca ggtaaggctt aattttctca
    22321 tattgcgcat agaattgtcc catgtctacc accaaatcgc ggatcaccgg taaacctggc
    22381 agcgggcgaa tcacaatctt cttgcccggc tggttgagtg ccgaaatcgg ggtaatacag
    22441 gccagaccat tcttgccgtt catgttcaga ccgtcggaac cgcacacacc ttcacggcag
    22501 gagcggcgga acgacaggct gggatctttc tctttcagct ggataagcgc atccagcagc
    22561 atcatgtcgc gaccttcttc cgcttccagg gtgtaatcct gcatacgcgg agcatcatca
    22621 acatccgggt tatagcgata aattgaaaac tcgagtctca ttttcctgtc tccgcattag
    22681 taagtacgaa tcttcggcgg gaatgccggg cgcagtttcg gttccatgtt gacgcttcgg
    22741 cgcgtcatgg attccgactc aggcagatac agggagtggc acagccagtt ttcatcatca
    22801 cgatccggga agtcgaagcg gctatgtgcg ccacggcttt cggtacggaa gttggcagaa
    22861 acagccgttg catacgccgt ttccatcagg ttatccagtt ccaggcactc aacgcgctgg
    22921 gtgttgaact cgctggaagt gtcatccaga cgggcatttt tcagacgctc gcggatcact
    22981 ttcaactgct caagcccttt cgccatcgca tcaccttcac ggaagaccga gaagttatgc
    23041 tgcatacatt cttgcagcgc tttacggatc gccaccggat cttcaccgtt acgattattg
    23101 ttccaccggt tcaggcgatc cagagacgct tcaacatcag actcgctggc atcgtgcagt
    23161 gcgccctgct cggcgataga ctcttgcaga tgcagacctg ccgcgcgacc aaagaccacc
    23221 aggtccagca gcgagttgcc gcccagacgg ttagcgccgt gtaccgatac acaagcgatt
    23281 tcaccaacgg caaacagtcc cggaacaacc acatcttcgc ctttctcatt cacagtcagc
    23341 gcctgaccgg taactttggt cggaataccg cccatcatgt agtgacaggt tgggataacc
    23401 ggaatcggct ctttcaccgg atcgacgtga gcgaaggtac gggaaagctc caggataccc
    23461 ggcagacggg attcgagaac ttctttaccc aggtgatcga gtttcagttt cgcgtgtggc
    23521 ccccacggac catcacagcc gcgaccttca cggatttcaa tcatgatgga acgcgccacc
    23581 acatcacggc ccgccaggtc tttggcgttc ggcgcataac gctccataaa gcgttcgcca
    23641 tgtttgttca gcagataacc gccttcacca cggcaacctt cggtgaccag tacgcccgca
    23701 ccggcaatgc cggtcgggtg gaattgccac atttccatat cctgcaccgg tacgcctgcg
    23761 cggatagcca tgccgacacc gtcgccagtg ttaatgtggg cgttagtggt ggactgataa
    23821 atacgccctg ctccgccagt cgccagcacg gtagcgcggg ctttgaaata aaccacttca
    23881 ccggtttcga tgcacagtgc ggtacaaccc accaccgcgc catcctggtt tttcaccaga
    23941 tccagcgcat accactcgga gaaaatggtg gtgtggtttt tcaggttctg ctgataaagc
    24001 gtgtgcaaca gtgcgtgacc ggtacggtca gcagccgccg cagtgcgtgc cgcctgctcg
    24061 ccgccgaagt ttttcgactg accgccaaac ggacgttgat agatacgacc atcatcgaga
    24121 cgcgagaacg gcaggcccat gtgttcgagt tccagaatcg cttccggccc ggttttacac
    24181 atatattcaa tcgcgtcctg gtcaccgata tagtccgacc cttttacggt gtcgtacata
    24241 tgccattccc agttatcttc atgggtatta cccagcgcaa cggtaatgcc gccttgcgca
    24301 gaaacggtat gggaacgggt cgggaagact ttagagagca gggcacaggt ctggccgctc
    24361 tgggaaattt gcagcgccgc gcgcatacct gcgccaccgg caccaatcac aactgcatca
    24421 aattctctga ctggcaattt catcacacac cccacaccac aacgaatcca taaatcacgt
    24481 aaaccaccag tgcaacgaca atcaccagtt gcagcatcag gcgcaaggcc agcggtttaa
    24541 cgtagtcggt caacacctgc cacatgccga tccaggcatg aatcaagata gaaaacagcg
    24601 ccagcagggt gaacactttg gtgaacgcag aggcgaagaa accgatccag acttcatatg
    24661 tcagttcgcc actggtggcg aaaaaaccga ccatataaat gatgtagagc gtcaggacga
    24721 tagcggtagc gcgaacgagg atgaaatcat gtacgccatt gcgtcctaat gcggaggcgt
    24781 tgcttaccat acgaggactc ctgcgagaag tgaaagcacg acagtaataa caaaggagat
    24841 tttggcggag cgtttacccg cttcgaatgt ttcttccaga tagccaaaat ccatcatcat
    24901 gtggcgaata cctacgacga cgtgatacgc cagagcggta aggatgcccc acatgataaa
    24961 tttgacgaag aagctgccca taatcgcgga agcttgctcg aaaccttcag gggaagagag
    25021 gctggtaccc agaagccact gcaggatgcc cactgcaaca aaggtgatca caccggaaac
    25081 gcgatggaga atggacgcta tcgccgtgat ggggaaccgg atggtctgta ggtccagatt
    25141 aacaggtctt tgttttttca catttcttat catgaataac gcccacatgc tgttcttatt
    25201 attccctggg gactacgggc acagaggtta actttctgtt acctggagac gtcgggattt
    25261 ccttcctccg gtctgcttgc gggtcagaca gcgtcctttc cataactgcg cgtcatgcaa
    25321 aacactgctt ccagatgcga aaacgacacg ttacaacgct gggtggctcg ggattgcagg
    25381 gtgttccgga gacctggcgg cagtataggc tgttcacaaa atcattacaa ttaacctaca
    25441 tatagtttgt cgggttttat cctgaacagt gatccaggtc acgataacaa catttattta
    25501 atttttaatc atctaatttg acaatcattc aacaaagttg ttacaaacat taccaggaaa
    25561 agcatataat gcgtaaaagt tatgaagtcg gtatttcacc taagattaac ttatgtaaca
    25621 gtgtggaagt attgaccaat tcattcggga cagttattag tggtagacaa gtttaataat
    25681 tcggattgct aagtacttga ttcgccattt attcgtcatc aatggattct ttacctgcaa
    25741 gcgcccagag ctctgtaccc aggttttccc ctctttcaca gagcggcgag ccaaataaca
    25801 aacgggtaaa gccaggttga tgtgcgaagg caaatttaag ttccggcagt cttacgcaat
    25861 aaggcgctaa ggagacctta aatggctgat acaaaagcaa aactcaacct caacggggac
    25921 acagctgttg aactggatgt gctgaaaggc acgctgggtc aagatgttat tgatatccgt
    25981 actctcggtt caaaaggtgt gttcaccttt gacccaggct tcacttcaac cgcatcctgc
    26041 gaatctaaaa ttacttttat tgatggtgat gaaggtattt tgctgcaccg cggtttcccg
    26101 atcgatcagc tggcgaccga ttctaactac ctagaagttt gttacatcct gctgaatggt
    26161 gaaaaaccga ctcaggaaca gtatgacgaa tttaaaacta cggtgacccg tcataccatg
    26221 atccacgagc agattacccg tctgttccac gctttccgtc gcgactcaca tccaatggca
    26281 gtcatgtgtg gtattaccgg cgcgctggcg gcgttctatc acgactcgct ggatgttaac
    26341 aatcctcgtc atcgtgaaat tgccgcgttc cgcctgctgt cgaaaatgcc gaccatggcc
    26401 gcgatgtgtt acaagtattc cattggtcag ccatttgttt atccgcgcaa cgatctctcc
    26461 tatgccggta acttcctgaa tatgatgttc tccacgccgt gcgaaccgta tgaagttaat
    26521 ccgattctgg aacgtgctat ggaccgtatt ctgatcctgc acgctgacca tgaacagaac
    26581 gcctctacct ccaccgtgcg taccgctggc tcttcgggtg cgaacccgtt tgcctgtatc
    26641 gcagcaggta ttgcttcact gtggggacct gcgcacggtg gtgctaacga agcggcgctg
    26701 aaaatgctgg aagaaattag ctccgttaaa cacattccgg aatttgttcg tcgtgcgaaa
    26761 gataaaaatg attctttccg cctgatgggc ttcggtcacc gcgtgtacaa aaattacgac
    26821 ccgcgcgcca ccgtaatgcg tgaaacctgc catgaagttc tgaaagagct gggcaccaaa
    26881 gatgacctgc tggaagtggc tatggagctg gaaaacatcg cgctgaacga cccgtacttt
    26941 atcgagaaga aactgtaccc gaacgtcgat ttctactctg gtatcatcct gaaagcgatg
    27001 ggtattccgt cttccatgtt caccgtcatt ttcgcaatgg cacgtaccgt tggctggatc
    27061 gcccactgga gcgaaatgca cagttacggt atgaagattg cccgtccgcg tcagctgtat
    27121 acaggatatg aaaaacgcga ctttaaaagc gatatcaagc gttaatggtt gattgctaag
    27181 ttgtaaatat tttaacccgc cgttcaaatg gcgggttaat ttttatatgc ctaaacacaa
    27241 aaaattgtaa aaataaaatc cactatcgga cattcatata ttctaaaaaa aaatacatcg
    27301 gtataaatta taaacaaata gatgattttt aatcatctat cttcctctca gttacatgaa
    27361 taaaccacca aatatagcgc acccctataa ttcccaatac tttattcatc aaacatttaa
    27421 ttcataccat caattaatta cttttacatt taataaaatt aataatattt ttaagaaaaa
    27481 ctaaaaacat tcgacaatca aataagatat tcttgcaccg atatactttt ttcttttctg
    27541 ttaaaggact cagttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
    27601 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
    27661 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
    27721 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
    27781 ctgctcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
    27841 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
    27901 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
    27961 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
    28021 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
    28081 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
    28141 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
    28201 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaatctga
    28261 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
    28321 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
    28381 agcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
    28441 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
    28501 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
    28561 tgaatattag tattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
    28621 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
    28681 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
    28741 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcatactgat agcgatgata
    28801 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
    28861 gttcaaaatt aaccctcggc gaaacggatt ttagtcccaa tattttcgac ggtttttctt
    28921 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
    28981 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
    29041 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
    29101 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
    29161 aggtttcagc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
    29221 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
    29281 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cagcggcctg ctgctttctg
    29341 gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg cttggtgcgc
    29401 tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac gagcggggct
    29461 taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg atttccctcg
    29521 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg gatcacaaat
    29581 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
    29641 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
    29701 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
    29761 atatctctat ttctacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
    29821 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
    29881 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
    29941 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga gcccaggtga
    30001 gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt acctatgccg
    30061 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
    30121 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
    30181 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
    30241 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
    30301 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
    30361 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
    30421 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
    30481 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
    30541 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
    30601 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
    30661 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
    30721 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
    30781 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
    30841 cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacaca
    30901 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
    30961 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
    31021 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
    31081 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
    31141 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
    31201 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacaccgg
    31261 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
    31321 gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
    31381 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgtcgttgaa gtctgcgctt
    31441 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
    31501 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tctgggaatc tgacgatatt
    31561 aaaataagat ctgggaatct gacgatatta aaattcccgt ctattgtgac aacaatacca
    31621 acggcaactt tgaaagcgaa catgtctacg catgggtaaa cccctacccg ggggtgcagg
    31681 atcgttatta tcaactcgga gtaacttaca atggcgttga ttatgacgcc aaccagggaa
    31741 aaagccgcat cgacactaac cagtgtatcg acagtaagaa cattgacatt tatacccctg
    31801 atgggatggc agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgaacc
    31861 tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa
    31921 agtacgctta gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac
    31981 cctactgccc aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat
    32041 tgcagcgtca atttttccat ttcaccggaa acacagatga ttgattttgg taaatttaat
    32101 ttgctggata ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca
    32161 caaaatgacc aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg
    32221 ttggttgaag aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat
    32281 gaaaacgcct cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac
    32341 atgttggtct acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc
    32401 gaggctgggc ccttcgatac agtggtgctt tttaaaatta actataactg atttgccatg
    32461 atgccctttg cagccggaaa gggcatctct ttcagaaaat tgctgatttt ccccttttcc
    32521 ttgattgctg ttattttttc ctagcccggc tttctttatg ctgtatcgat acaccaaaga
    32581 gaaaataatg agggagcgaa ggatgccagt tttgcagtgg ggaatgttat gtgtgctatc
    32641 acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcgctat tacttgggcc
    32701 gatgatcgcc gggatcatct tcagtatgcg cggaattact ctgcaacttc cccgctccgc
    32761 ttttctcgcc gcgcaggcca ttcttggctg catgattgcg caaaacctca ctggttcgat
    32821 tctcaccacg ctggccgcca actggccgat cgtgcttgct attttactgg tgacgctgct
    32881 ttccagcgcc atcgtgggtt ggttattggt gcgctatagc tcactgcctg gaaataccgg
    32941 tgcctggggt tcttctccag gcggcgcagc ggcaatggtc gctatggcgc aagattacgg
    33001 cgcagatatt cgcctggtgg cgtttatgca atatctgcga gtgctgttcg tcgcgggggc
    33061 cgctgttctg gtcacccgca tgatgctggg tgataacgct gaagcggtta accagcaaat
    33121 cgtctggttc ccgccagtga gcattaatct cctgcttacg attttgctcg cagtcgttgc
    33181 cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc cgatgctggc
    33241 gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccggaat ggctgctggc
    33301 gatggcgtat atggcaattg gctggcggat tggtcttggt ttcgataaac aaatcttact
    33361 gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc tggctatttg
    33421 tgcaggtatg gcgtggggac tgacccggtt tatgcatatt gattttatga ctgcctacct
    33481 cgccaccagt cccggcgggc ttgataccgt ggcggtcatc gccgcaggga gcaatgccga
    33541 tatggcgctt attatggcga tgcaaaccct gcgcctgttc agtattttgc tgacggggcc
    33601 agccattgcg cggtttattt caacctatgc gccgaagcgg tcggcctagt gctggcagcc
    33661 tggacaccag taaaacgggc gcgaggacaa cgtggttttc tcaatgatgc cgccacagcg
    33721 ttcgcacagt tcgccatcac gatgaaaaac cttaaagcga aacagcgccc catgatgctt
    33781 attctcatcc acctgccccc gcgtagcgta agaaaaccga ggaatctcca gtaacgcgtg
    33841 tgcgagtgca tccagttgcg ccgcattgag atctttcgct ttatgatttc cagtcaaccc
    33901 aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa acgcctgatc
    33961 gagcagtaat cctgcaaact gacggttacg aaagcgcggc gacaataacc gttcctttac
    34021 cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta aaaacggatg
    34081 cgtggtcagt tgttccggcc tcaacatctc aatatccgag gcgctataaa gcagaatggt
    34141 tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct cttcgccggt
    34201 atcaaccacg cgccagacgc cgtaaagctg attatggctg tagagcgtta aaccgttggg
    34261 aaaatgagtt aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt gaccgataag
    34321 ttgtgattga taagttttta actgcgggaa ggcaaaccag acatcagtta gtggtttgcc
    34381 tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt caggcatggt
    34441 gatatccttt tttcattctt tattaagcgg ggttatgctg cgacaacaat cccctttttg
    34501 gcaaatgtag ctcgtaatcg gcgggcgaac gccagagcgt gctcgccgtc gccatgcagg
    34561 cagaccgttt gcgccgcgac cgttgcccat tcgccggtga tacttttgac tctgccgtgt
    34621 tgcaccatct ccagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa cgcgcctggc
    34681 tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa cacttcctcg
    34741 cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc cagcccaacg
    34801 agaaccaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc cagttgtgct
    34861 tctttcgccg cctggttgta caacatgccg tgcggtttga catgacgcat tacgccgcct
    34921 tgcgcacggg caatcgttgc cagcgcgcca atttgataca gcgtctgggc gtaaacggtt
    34981 tccggcggca gctgcatggc gctgcgacca aaattttccc tgtcgggaaa actcgggtga
    35041 gcgccaatcg cgacaccatt ttttattgct tcacgcacgc aagcctgcat ggtttgcgca
    35101 tcgcctgcat gaaatccaca agcaatattg gcagaggaaa ccagcgttaa tagctctgcg
    35161 tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca
    35221 cgctaattgt tcaaaataac gttgctgatc ttgccgcgct ttcagtgcct cttccagtga
    35281 acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt acatatcagc
    35341 ctcaatgata caggcaatac gtggataacc accggtggtc tgtgcgtcgt tcatcaacac
    35401 aatcggctgc ccgttatgcg gcacctgcac cacgctcggt aacaaaccgt gagataacag
    35461 ttcgcgatcg gtgatgcgtt ttaaaatttg cccctgtaaa cgatagccca ttcggttact
    35521 ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc gatcgaactc
    35581 atgatattcc ggccccggca aggcgcgaat gcggttgccc cacagcagct gtttaacgcc
    35641 ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg agtcagtcac catccttcag
    35701 taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg accccattac
    35761 cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg
    35821 tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccggtcc agacggcgtt
    35881 atcatccaac cgcgcttcgc aacctgcacc cgtcagggca aaccacccat cagtttcgaa
    35941 ctcaactgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt cattacccac
    36001 cagcaggtta gcaatgcgca gcgcaggcat atccagtgcg ccacagtggc tgatacccga
    36061 ctggcaaaaa ccgtgacgac cgccatcctg cacagtggtg tacatgcccg cacgaataat
    36121 cttcagcata ttccctcctt ctgcggtaca aatcgcacgc tgtctcccgg acgtaataag
    36181 atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat caactgccag
    36241 ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc gacagaacct
    36301 gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc
    36361 ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc cacggaggag
    36421 tgcaattcaa caacctgttt ttcgctcaac ccgcaatacg ccgcgaccac cgccaaatcc
    36481 ggtccgcctg caccaccgta aaccaccgga atttcaataa agcgagactc aggttccagg
    36541 gcctcgctct cctcccacca gcgttgcaaa cgctctatgg catccagcgc cagcgactca
    36601 ggattacgca aaatcaccgt gatattgttc atgccgggaa tggcttcaac cacattcggc
    36661 atatccacca gacgctgcgc cagtcgccag atccgtttct ggctagccag cgtcaccggc
    36721 ggttccagtt ccagcactac cgccgtttca cctatcagat aacaacgcgc tcgttgcact
    36781 ttcgatccct ctgattattc gttatgcagg attaggaata tcaataaagg tcacatcaag
    36841 atcggtattt tcattcagcc actcgctcaa tgcgcgaata ccaccgcgtt cagtggcatg
    36901 gtgacctgca gcataaaaat gcaatccctg ctcgcgggct gaatgaatgg tctgttcaga
    36961 aacttcgcca gtaataaaag catccacgcc aaaacgcgcg gcgctatcga taaaactttg
    37021 cccgccgccc gtgcaccagg cgacgcgctg gaccacctca ggtccggtat cgccacacca
    37081 taacggctta cgtcccagac gcgcttcaat ccaggaggct agttccattc ccggcacagg
    37141 catggtcagc tctccccacg gcaccaacgg ctcaatttcg cccatgaccg tgatccccag
    37201 taacgccgcc agctgtgcgt tattgcccag ctcaggatgt gcgtcaagcg gcaaatgcca
    37261 gccatacagg ttgatatcat tcgccagcaa cgtttttaaa cggttacgct tcattccgcg
    37321 aatgaccgga gactctcctt tccagaagta gccatgatgc acaatgaccg catctgcgcc
    37381 cagacgcact gcctcatcga gcaaagcctg gctggcggtg acaccggtaa caattttttg
    37441 caccgtctct ttgccttcca cctgcaaacc attcggcgcg taatcactaa tcgccgcgct
    37501 gttcagtttt tcgttaatca gttgttccag ttcggtgttt ttcatcttcg cgccttccgt
    37561 attctgatag ctacaccgtt aacatagcgt tacatcgcct gttcgtcgat agccaatctt
    37621 ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa attgtttata tcatcacgct
    37681 tcagctaatg tatatttatg caatcagaca gcatattgca taaataatca gatctgtatc
    37741 aaagttttaa ctttaaggaa acacaagcac gccgcgcaac tattacgtga cgttgccgtc
    37801 aattgtggaa ttattatgaa taaacacgca tcacaaccgc gcgctattta ctatgtcgtt
    37861 gcgctgcaaa tctgggaata ttttagcttt tacggcatgc gtgccctgct gattctctat
    37921 ctcaccaatc aactaaaata caacgatact cacgcctacg agttatttag cgcctactgt
    37981 tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg cggataaagt tctcggcaat
    38041 cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt gctgggtgcc
    38101 agtgagatcc atccgtcatt cctctatctg tccctggcga ttatcgtctg cggctatggc
    38161 ctgtttaaat ctaacgtaag ctgtctgctc ggcgagctgt atgagccaac cgatccgcgt
    38221 cgtgatggcg gtttctcgct gatgtatgcg gcgggtaacg tgggatctat tatcgcacct
    38281 atcgcctgtg gttacgccca ggaagagtac agctgggcga tgggctttgg cctggcggcg
    38341 gttggcatga tcgcgggtct ggtcattttc ttatgtggca atcgtcattt cactcatacc
    38401 cgcggcgtta acaaaaaagt actgcgtgcg acaaactttc tcctgccgaa ctggggatgg
    38461 ctgctggttc tgctggtggc aacgcctgca ctgattaccg tgctgttctg gaaagagtgg
    38521 tcggtatacg ccttaattgt cgcgactatt attggtctgg gtgtactggc aaaaatttat
    38581 cgcaaagcag aaaaccagaa acagcggaag gagctggggc tgattgtgac gctcaccttc
    38641 ttcagtatgt tgttctgggc cttcgcacaa cagggcggta gctcgattag cctttatatc
    38701 gaccgcttcg ttaaccgcga tatgtttggt tataccgttc cgaccgcgat gttccagtcg
    38761 attaatgcct tcgcagttat gctgtgcggt gtgttcctgg cgtgggtggt taaagaaagc
    38821 gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct tggcctgatg
    38881 agcgccggat tctgcattct gaccttaagc gcccgctggt ccgcaatgta tggtcactct
    38941 tctctgccac tgatggtgtt aggcctcgcg gtgatgggct ttgcggaact gtttatcgac
    39001 ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg gcgtgaccgg cgtattaacc
    39061 ggcatctaca tgctgctttc tggcgcgatc gcgaactatc tggcgggcgt gattgccgat
    39121 cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca actactccat caatgcatat
    39181 attgaagtat ttgatcaaat tacctggggc gcactggcgt gtgtaggact ggtactgatg
    39241 atttggctgt atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga gtcataatag
    39301 gccagcaccg atgcctgatg cgacgcttga cgcgtcttat caggcctacg tatttcctgc
    39361 aatttattga atttgcacaa atttgtaggc tggattaggc gttcacgccg catccggcat
    39421 ctgggctcga ttgcctgacg cgtctgttat ttccccttcc gcgccgcctc atacgctgcc
    39481 aacgtttgta ctctcgcctc tttgtgctcg actatcggtt gcggataatc cagcttcaca
    39541 cctgctttct gcgcccactt ccacggctca tgcaccgctt tccctggtac atcgcgcagc
    39601 tccggtaacc atcgacgaat aaactcgccc tcacggtcaa atttctcgcc ctgagttatc
    39661 ggattgaaaa tacgaaaata cggcgcagca tcagtacccg tagaagccgc ccactgccag
    39721 ccaccgttat tagctgccaa atcaccatca atcagctgcg acatgaaata tcgctcgcct
    39781 tcgcgccagt cgatcaacaa atctttcacc agaaaactgg ctgtaatcat ccgtagccgg
    39841 ttatgcatcc agccagtgct gttaagctga cgcatagcgg catcaataat cgggtatccc
    39901 gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc tttgccactg cacacgatcc
    39961 gtccaggcaa taaatggacg atgtttacac aacgaggggt aatacgtcat cagatgacgg
    40021 tagaactcgc gccagatcag ctcactaagc cagacactac cggccccacc gtccagcgcc
    40081 tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag gcgataaccc gcccgttgcc
    40141 aagctggcgg acaaacggct ggtgccttcc actgccggaa aatctcgttg ttgctcatac
    40201 tctccagcac cgttctcgca aaactggcgt aattgcgcaa tcgccgcttt ttcttccacc
    40261 gggaaatgcg cagtatcgaa agactgacga ggataattca gcgtaatgga tggcgcgggc
    40321 tttatcgatc cgctactacg aacttttggt gcagcgacgc actccggcat cccttcccgc
    40381 agccgtttca gccaggcatt cttaaaaggc gtaaagactt tgtacatctc gtgattgccg
    40441 gtcatcaccg cccccggcgg caggatcacg ctgtcatcaa atccttcaca caccacgtta
    40501 cgcagcgctc tttcaacgtc cacatcccgc gctcgctcat tcacctcata ctgatagtta
    40561 taaaaaagat gagtaacgct gttttccgcg cacacctgtt taactatttc gacactggcg
    40621 acaaagtcat ccacttcacg gaacaataaa ggaatacctt tttccgccag cgcgatttgt
    40681 agcccattca gttgagcatt gataagttca gcctgacgcg gcgacatgtt atgcgtctcc
    40741 cactggcgtg gtgtagcgat atacagcgcc agcacgcgtg cagacgaatt gcggcaggcg
    40801 gcagccagtg cgagattatc gtgcagacgt aaatcctggc gaaaccaggc cagatgggta
    40861 gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg ccaaagttcg aaataacgct
    40921 gccctgaaag ccaggcgtca ggataaagcg ccatatagtg tttgatacgc atcagcggcg
    40981 caagaagtgg ttgctcgcca cggcgataac tgtcgataag cgcagccagc tgctggcgtt
    41041 ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg catcaaaacg ttggtgtgat
    41101 cgcgcgggtt ggcgggatga gaaagcaaaa caattacgcg ttggcggtac tggttgtaaa
    41161 aatcatccag attttgccac tgatgaatat cagccacaaa cgggccaagt ttgcggtact
    41221 cgggctgaga atgggccaga aagacgagtt tatagcggct gtgataatcg agaagcgccc
    41281 cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg aagggcgaag atacggatta
    41341 gggtgctgtc atcaaatcgt tgtagattca ttactgctcc tgaacggtat acaggagtaa
    41401 gtgtaggcgt tggtaaatga tgtttgtgaa gttaatccgc tagcagggca agattacgaa
    41461 agcgcgctcc ccgcaagcaa ctaatcaagg tagttaatca tggctgaaca cgatattacg
    41521 ccagaagtca ccatttccaa aacacagcgc cgctataaag tcggctatgt cagagcccgc
    41581 caccaggacc gcaaaaccgg tatagcccgg tattattcac aacatcccag cctgcacctt
    41641 aagggcgact ggctgaaaga ggccggattt gataccggac ggggtgtcac ggtgaagatt
    41701 tcggaggggg gcctgaccat catcgctgac agtgacgagg tgcaggaact gcgggatggg
    41761 atgtttagtg tgctgaacga aagttaaata ttaaaaaagc tgggaagatt taaaaccttc
    41821 ctagcttttt attctgtctt attataatgg atactcatca tcttccgatg aatttataaa
    41881 ttgctccaag tttacatgaa gagtacttaa cctagttttt aactcattaa gtgaagcgat
    41941 aatttcatat tttccaacat aatcatatga tggatcgtat tcattatcta tgtcagaagc
    42001 taaaaatgat gatattcgta gtttaaattt agcttcatct ttattacatt cattggcata
    42061 aggtgaatgc cctatcatag catattcatc taatctaaaa ggataagatg gttttaattc
    42121 atcgatcaat gaaatttcaa tccatgttct atatccaggc attaatggac taatttcttt
    42181 gagcaaaacc tttccactta ataatgtatt gagattgttc attattatgt ctcccacggg
    42241 aaaaatatat gtctaacact tccaccatgt tgatgaactt gtaaatgtgg taaatcacca
    42301 tcaggatttc ctagtccatg acctgacact ctatatgaag gttttgttac agaaggatct
    42361 tttatatcat cccaatgata tgaacctgct tttgttttat ttccagtaag atattccatt
    42421 aagttcttag tgctggggcc tgactcacct gtcatatttt tataattatg acctaaatat
    42481 ctatccataa acaattgttc agcgtcactt ctagacctaa cagtaacttt ggtttcacta
    42541 ctatctaata aacgattcgc tttttggtta ctaacagcac atggagttaa tccaagtgga
    42601 tcagcatatt ttagaggatt aggtgcataa ctgtagagat ttaaaccgcc tctcaaccct
    42661 atcgggtcag ttaccatgaa ccgcccgaca tccggatcat agtacctgtg cagattgtag
    42721 tgcagccccg tttccctgtc gaggtactgt ccctggtagc gcaggttctg ctccaccccg
    42781 ccgtggtcct tctcctgtat cgtgttaccc cagacctgat accccgtctc ccacacgatt
    42841 ttcccgtcgc tgtccgtcat ctcttccggc gcgccgttca catccgtgtg aaaatacagt
    42901 atccggttcg ggccctcttt gcccgcctta tccacccgcg ccagcggttc ataactcccc
    42961 tggtccctgt agatgtacag gctgcctgtt ccctccggcc tgctctcctg cgccagccgc
    43021 attccgtccc ataaaaattt tgtccggttg tacggcttac cgtcagggct gatgtgctgt
    43081 ttttccgtcc gcctccccag tgcatcgtac acataccggt atgtcccccg cgctgtcgtc
    43141 acttccacca tccggtgttc cgcatcataa cggtaactct gcgttccgct ccggccctcc
    43201 ttttccaccg cacggccaaa ttcgtcataa cggtagtgca caccacggta agacatcagc
    43261 cggttgaacg gcaccacgct gttttctgcc gtggcccttt cccccgcgcg cctccccagc
    43321 aggtttcccg ccgcgtcgta ctgccagctc gccagatacc cctcatcctc cgaacgacac
    43381 gccgtgatgc gccccgtggg gtcgtagaag taatccgttt ccccccgccg gctgtgccgc
    43441 gtcttcagca gttcatcctc gccactgtag ctgtactgcc ggtccgtgaa cgtctccggt
    43501 accaccccgc gcatcccgct gtatgtctcc ctgcgcttca gccgcccgcc ggggtcgtaa
    43561 agctgccgcg tgctcagtcg cccctggctt ctgagcactt cacggtgcag gtggtcgcgc
    43621 gtgaactcgc tgatgacctc ccggtccaga tttatctgct ggagatggcc gctgccgtag
    43681 tacagatggc gctgcgttct gccgtccgga aaggtggtgg cagtccggtt gcccagttta
    43741 tcgtactggt gggtcaggat gccctgcgcc gtctcctcgc tcagcatccg ccccagtctg
    43801 tcgtagctga agcggataac ttccggcgcg tcagtttcgt gccgctggcg acggatttcc
    43861 agtagctggt ctgccgcatc atggacgtat tccgtacggc catcggctgt ctcttttgcc
    43921 gtgagccggc ccgccgcatc acgcgccagc cggtggcgga tttcaccgcc ccggtccccg
    43981 gtgaacacca cttccgtgac cgcgttcagc gcgttgtagc ggtacgactg gcggcagccg
    44041 tccgggttta tctgctccgc cagacggtcc agcacatcat ggcggaaccg gtagcgttct
    44101 ccgttctcgt tcgtgagcgt gaccagacgc ccgtaagcgt cgtacccgaa tgccgtcctg
    44161 cgccccatcg cgtccgtccg gctgcgcacc cggccctgcc cgtcccgctg ccagcgcgtt
    44221 atcctcccca tcgggctggt gtactttacc agcctgcccg ccgcgtctga ctggtaattc
    44281 accgtgcggc cgtccgccag tatcacctcc gtcagatgac cgctgtcgtc atacccgtag
    44341 cgcgtgctgt ggctctgcgc atccgtcatt ctgataagcc tcgtgcgttc gtcgtagaac
    44401 cacgccgtct gcgagccgga acagtccgtg tgccgcatga gctgcccgtc ttcattccac
    44461 tggaggtatt tattcccccc gcgcgcatcc gttatctgca ccacctagcc gtggtggtca
    44521 tactgatacc gggtgttctg ctgcagcggg tcggtcacgg caagaaggtt gccgcgttca
    44581 tcgtacacac agcgccaggt gtttcccgcc gcgtccacct ccgtttccgg ctggtgccac
    44641 accgggtgcc actgcgcctg ctccacccgc cccagcgggt cgtgcgtctc cgtgatatgc
    44701 ccctgcaggt catacacata gcgccatttg ccgccctgag ggtcggtcat ccccagcaac
    44761 agtcgttctt cgtcgctcca gcggaatgtc gtcacctgtc cggcttcgtc ctggtacgcc
    44821 gtcacctgat tctgcgcatc ccactggtga taacgtgtgc gccccagtcc gtccgtcacg
    44881 cacagcctgc gctgcgccag gtcccacgcc agccggtact gttcaccgct gtccgtgtgg
    44941 tgccccgtca cccgccatgc cgcgtcatcc ggaccttcat accagcccca ctggtaataa
    45001 tacccttcgc cgcccggctg gcggtgcagc gtcatgcagt ggttttcgtc gtaagcaaac
    45061 tcacgcagca ggtgccccgt gctgtcgtac acgccgttca ggtggccggc ctcgtcatag
    45121 ctgtaacgac gcagcagtaa aggtgcagtc tcagggaaat gctgataaat ctgtgtcacc
    45181 cgccggggat gcgccgccag ttcgtaatac agacggatgc acggacgctg ctgttcgccg
    45241 ctgatttcca caatgcggcc ccggtcgtcg tagtacagat tcagacagtt gctgttacgg
    45301 tcgcccagca ttttcagccg ctggcgctgc gggtggtgcg ggtcagcctc aaacagaaaa
    45361 cactgcccgt catcgctgct gataagccag tgcccctgct caccacgcct taccgccagc
    45421 ccttcaccgg ggctgtaaaa cccgcccccc ggcagtacgg cttccatatc caagcgtcgc
    45481 cccatgcccg acacaaaggt catgcagttt tcgtctgcgc tacccggtgt gcacacgagg
    45541 ctgactttat acattacact ccagccctga ccaaacattc cttccgtacg ttcgtcacga
    45601 ctgctgtaca cgcgctgcca gtccagcgga aagtggcccg gcagactgaa atcgaagtca
    45661 tcgtcatcgg ccaggtattt ggccccggtg gcggcattga ccggatgcgg aagggcactg
    45721 gtggccttct gcgtcagcat gctcatcccg acactggcag caaaacaggc gattttagaa
    45781 agcattttgc ccgggcgaag aaacatcagc gccacggttg ccaccagggt tatctgcgac
    45841 ctgccgcttc tgatatccct gaccaccagt aacggaccgc caatgcgcac atccggtgac
    45901 acaaacgccc cgttccccgg ctcattcacc accctggcct cgcaggtaca gcgtgccccg
    45961 ctgcgtgtcg ccggctggct gttgatacgc acatcccgcg cgccttccgc gatatattcc
    46021 gctgcgggcg gctcgtcatc gcctttgaac agattactga tgctgccaat aaccgcattc
    46081 actccctgaa aggcggtttc cagcataccg cccggattca gcaggttaag ggccgttccc
    46141 agggggtcat ccagaaaggc ggcttttttt tgtgcgagaa actgcgtcag tgaggaagga
    46201 tgtttatggc agtcgatttt atcgtcaggg gtcggggtgg atcccggggc cgcactggcc
    46261 acagtgggct gccatagttc cgacacaaaa tgtgtcacgc catccaccat ttcagaacgc
    46321 ccttcttccg tactgagctg gtcgtgcaga gcccgggaca tcagcacagg ccctgtcacc
    46381 gggttaaccg ccagggctat ggcccccgcc atttccagat attccccggc cacatcccgg
    46441 aacgttctgg catcctcctg acgctgcgct tcttcctgtt ctgccttcgc ctgcgcttcc
    46501 tgcgcctcta tgtcctgccg cgatgccgca gtcgccgctg cccgcgcggc aggtttgctg
    46561 ttgatccatg tgtcaccaga gccggtgagg atgtaacctt caatctgagg cgggaaaagc
    46621 atatttgcga gggctgaaca ggcttcgctg accttttctg tcacgccggt gtacaccatc
    46681 gccacacccg ccacaaaacc ggtcagcacg acggccgtgc accccatcgt gatccccact
    46741 gacgtcgcca gtaacgaggt tgtgccgaca atgagggcat caataacgac atttgcggcc
    46801 acctccagca ccgcccccag cacatctgcc ataaacgacg tatgcagcag ggcatcaccc
    46861 tgtcttgccg cccagaatgc ctcttccatg tcccgttccc tgtatgatta attcgtcctg
    46921 acttcctgcc gcatcctgaa gcgcagattc tttttaattt cagcccagcg cgtcatatct
    46981 gccggggtca gcggtgtggt tctggcgtag gtgaaggcca tcatgacccg ctcacccggc
    47041 aatacaaatg ccacctgaca ctggtacacc tgctcgttac cccggagaaa ctggctctgt
    47101 atttccaaac cgcctcgttg agataatatt ccgtcataat gtaaatagtc cgttttaata
    47161 aaggaattat ttattgacag gttctgtttt tttaatcatg aatgtaatac ccatcaccgc
    47221 tttacagcac actttatctt tccagccata atcactaata atattaagca attagcaata
    47281 tttacaacga ccagagcgga tacatagtta tgtatttaac aatcccaata tattcactat
    47341 gcaaataaaa ccactagtaa cagacacgct ac
//
LOCUS       DAJTUN010000015        47102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTUN010000015 DAJTUN010000000
VERSION     DAJTUN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.015"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..218
                     /locus_tag="OO470_000822"
     CDS             <1..218
                     /locus_tag="OO470_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250161.1"
                     /translation="GELKTTDELDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATL
                     DVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1039..1815)
                     /gene="sseB"
                     /locus_tag="OO470_000823"
     CDS             complement(1039..1815)
                     /gene="sseB"
                     /locus_tag="OO470_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6250162.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1957..3240)
                     /gene="pepB"
                     /locus_tag="OO470_000824"
     CDS             complement(1957..3240)
                     /gene="pepB"
                     /locus_tag="OO470_000824"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6250163.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3299..3499)
                     /gene="iscX"
                     /locus_tag="OO470_000825"
     CDS             complement(3299..3499)
                     /gene="iscX"
                     /locus_tag="OO470_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6250164.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3511..3846)
                     /gene="fdx"
                     /locus_tag="OO470_000826"
     CDS             complement(3511..3846)
                     /gene="fdx"
                     /locus_tag="OO470_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6250165.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3848..5698)
                     /gene="hscA"
                     /locus_tag="OO470_000827"
     CDS             complement(3848..5698)
                     /gene="hscA"
                     /locus_tag="OO470_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6250166.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5715..6230)
                     /gene="hscB"
                     /locus_tag="OO470_000828"
     CDS             complement(5715..6230)
                     /gene="hscB"
                     /locus_tag="OO470_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6250167.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6326..6649)
                     /gene="iscA"
                     /locus_tag="OO470_000829"
     CDS             complement(6326..6649)
                     /gene="iscA"
                     /locus_tag="OO470_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6250168.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6666..7052)
                     /gene="iscU"
                     /locus_tag="OO470_000830"
     CDS             complement(6666..7052)
                     /gene="iscU"
                     /locus_tag="OO470_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6250169.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7080..8294)
                     /gene="iscS"
                     /locus_tag="OO470_000831"
     CDS             complement(7080..8294)
                     /gene="iscS"
                     /locus_tag="OO470_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6250170.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8406..8894)
                     /gene="iscR"
                     /locus_tag="OO470_000832"
     CDS             complement(8406..8894)
                     /gene="iscR"
                     /locus_tag="OO470_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6250171.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9164..9904)
                     /gene="trmJ"
                     /locus_tag="OO470_000833"
     CDS             complement(9164..9904)
                     /gene="trmJ"
                     /locus_tag="OO470_000833"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6250172.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10023..10826
                     /gene="suhB"
                     /locus_tag="OO470_000834"
     CDS             10023..10826
                     /gene="suhB"
                     /locus_tag="OO470_000834"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6250173.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            10971..11825
                     /locus_tag="OO470_000835"
     CDS             10971..11825
                     /locus_tag="OO470_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6250174.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12016..13296
                     /gene="csiE"
                     /locus_tag="OO470_000836"
     CDS             12016..13296
                     /gene="csiE"
                     /locus_tag="OO470_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6250175.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13288..14427)
                     /locus_tag="OO470_000837"
     CDS             complement(13288..14427)
                     /locus_tag="OO470_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6250176.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14587..15477)
                     /gene="hcaR"
                     /locus_tag="OO470_000838"
     CDS             complement(14587..15477)
                     /gene="hcaR"
                     /locus_tag="OO470_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6250177.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15613..16974
                     /locus_tag="OO470_000839"
     CDS             15613..16974
                     /locus_tag="OO470_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6250178.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16971..17489
                     /locus_tag="OO470_000840"
     CDS             16971..17489
                     /locus_tag="OO470_000840"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6250179.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17489..17809
                     /locus_tag="OO470_000841"
     CDS             17489..17809
                     /locus_tag="OO470_000841"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6250180.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17806..18618
                     /gene="hcaB"
                     /locus_tag="OO470_000842"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17806..18618
                     /gene="hcaB"
                     /locus_tag="OO470_000842"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6250181.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18628..19830
                     /locus_tag="OO470_000843"
     CDS             18628..19830
                     /locus_tag="OO470_000843"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6250182.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19927..20349
                     /locus_tag="OO470_000844"
     CDS             19927..20349
                     /locus_tag="OO470_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6250183.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20397..21269)
                     /locus_tag="OO470_000845"
     CDS             complement(20397..21269)
                     /locus_tag="OO470_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6250184.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21281..22375)
                     /locus_tag="OO470_000846"
     CDS             complement(21281..22375)
                     /locus_tag="OO470_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6250185.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22408..23406)
                     /locus_tag="OO470_000847"
     CDS             complement(22408..23406)
                     /locus_tag="OO470_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6250186.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23431..24942)
                     /locus_tag="OO470_000848"
     CDS             complement(23431..24942)
                     /locus_tag="OO470_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6250187.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24965..25948)
                     /locus_tag="OO470_000849"
     CDS             complement(24965..25948)
                     /locus_tag="OO470_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6250188.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26045..29146)
                     /locus_tag="OO470_000850"
     CDS             complement(26045..29146)
                     /locus_tag="OO470_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6250189.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29153..29326)
                     /locus_tag="OO470_000851"
     CDS             complement(29153..29326)
                     /locus_tag="OO470_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250190.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29444..30637
                     /locus_tag="OO470_000852"
     CDS             29444..30637
                     /locus_tag="OO470_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6250191.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30835..32088)
                     /gene="glyA"
                     /locus_tag="OO470_000853"
     CDS             complement(30835..32088)
                     /gene="glyA"
                     /locus_tag="OO470_000853"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6250192.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32415..33605
                     /gene="hmpA"
                     /locus_tag="OO470_000854"
     CDS             32415..33605
                     /gene="hmpA"
                     /locus_tag="OO470_000854"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6250193.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33650..33988)
                     /gene="glnB"
                     /locus_tag="OO470_000855"
     CDS             complement(33650..33988)
                     /gene="glnB"
                     /locus_tag="OO470_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6250194.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34049..35383)
                     /gene="glrR"
                     /locus_tag="OO470_000856"
     CDS             complement(34049..35383)
                     /gene="glrR"
                     /locus_tag="OO470_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6250195.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35373..36086)
                     /gene="qseG"
                     /locus_tag="OO470_000857"
     CDS             complement(35373..36086)
                     /gene="qseG"
                     /locus_tag="OO470_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6250196.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36251..37678)
                     /gene="qseE"
                     /locus_tag="OO470_000858"
     CDS             complement(36251..37678)
                     /gene="qseE"
                     /locus_tag="OO470_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6250197.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38254..42141)
                     /gene="purL"
                     /locus_tag="OO470_000859"
                     /gene_synonym="purI"
     CDS             complement(38254..42141)
                     /gene="purL"
                     /locus_tag="OO470_000859"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6250198.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42400..43956
                     /gene="mltF"
                     /locus_tag="OO470_000860"
     CDS             42400..43956
                     /gene="mltF"
                     /locus_tag="OO470_000860"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6250199.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43953..44489)
                     /gene="tadA"
                     /locus_tag="OO470_000861"
     CDS             complement(43953..44489)
                     /gene="tadA"
                     /locus_tag="OO470_000861"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6250200.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44514..45149)
                     /gene="yfhb"
                     /locus_tag="OO470_000862"
     CDS             complement(44514..45149)
                     /gene="yfhb"
                     /locus_tag="OO470_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6250201.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45358..46206
                     /locus_tag="OO470_000863"
     CDS             45358..46206
                     /locus_tag="OO470_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6250202.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46545..47099)
                     /gene="pinE"
                     /locus_tag="OO470_000864"
                     /pseudo
     CDS             complement(46545..47099)
                     /gene="pinE"
                     /locus_tag="OO470_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT        11232 a        12908 c        11828 g        11134 t
ORIGIN      
        1 aaggcgaact aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct
       61 atgacaaacc gattatcgtc agctgcggct ctggtgtaac ggcagccgtg gttttgttag
      121 cactcgcgac gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg
      181 gcgcgcgggc agatttaccg gttgagccag tgaaataagt attttacagg caataaaaaa
      241 ccgccgaatt tggcgttttt ttattgctgg tctggttcgc ggcctttcca gcaggttgac
      301 ttgtgttaca catgagcaac gcaggtgctt cacagcaaaa caatactcac cagtaactct
      361 ctttttgtca agcaaaagag agtaattatt gtttatttag cgtattatcg acaccggccc
      421 tttccgccgt gttcggtaat aaaataacct ggcttattag tccgaattca gacaaatata
      481 aacaaatcct gctcaaaatt aaaaattcta accggtaaaa gatattactt aaacatgtaa
      541 atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag gcggttattt gttgctggtc
      601 cggttcgcgg cctttccagc aggttgtatt accgtagtaa tgcaagcgcg tctcagcgga
      661 gacaatactc gccagtaact ctctttttgt caagcaaaag agagttatta ttgttctgtt
      721 agtgtattat ccactgcggc cctttccgcc gtctcgcaag cgggcgctgg cttaaggaaa
      781 ggatgttccg cgaccgttag agcaagcgcg tctcagcgct tgctatcgcg gcaatatcgc
      841 cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata cggtgcttct
      901 gtatcgtact tattgtttct ggtgcgctgt tttcccaagt aaataataac cggagtctct
      961 ccggcgacaa tttactggtg gttaacaacc ttcagagcag caagtaagcc cgaatgccgc
     1021 cctttagggc ggcatatttt agattatccg gttttgttta aagtcacgca aaaaaccacc
     1081 ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata aagtggctaa ttcctttttc
     1141 cccttttttc acctgacaaa tatcgattgg ttcatcgcca ggtaatgtat cggtcgctac
     1201 acttcccgtc gcctgaataa tttcttcgat atcaccatcg gcttcaatgc caataagtaa
     1261 attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct tcaccggctt
     1321 aatggtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct ctgcgacttc
     1381 cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg gatttccctc
     1441 ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg gcagtttggc
     1501 attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg gcattacgac
     1561 aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct aacgaggtga aaaaaggaat
     1621 gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcactat cttcaaccac
     1681 agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta gagtacggaa
     1741 aaaggccggg cggtgcgccg gttcggttgc tgctttttcc agcaggtctt caagttcgtt
     1801 ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcgcaaa tgcctgatgc
     1861 gctacgctta tcaggcctac aagggattcg caatttgttg aatttgcaga atttgtaggc
     1921 cggataaggc gtttacgccg catccggcat attagtttac gccgttaaca gattagctat
     1981 cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt tcaaccggcg ctttacggta
     2041 agtcgccgag cagtcgatat gcagccagcc ttgctgatag ttctcaacaa agtgcgacag
     2101 gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg cttccggtat tgttcagttc
     2161 ggcaaagtta gacggcagct ggctgcggtg gaattccgcc agcggcagac gccagaatgg
     2221 ttcgttctct tgtgaggcac tcgccagtaa acgaccggca agcgcatcgt caaaactgaa
     2281 cagcgcgtga taatcattac ccagcgcagt tttcgccgcc ccggtgaggg tcgccgcatc
     2341 aatgatcatt tccggtttct gcgcactggc atcaatcaga ccatcggcaa gcaccagacg
     2401 cccttcggca tcagtgttca tcacttcaac ttttttaccg ttgcgatagg tgatgatatc
     2461 gcccagcttg aacgcattgc cgctaatcag gttatccgca cagcagagga acagcttcac
     2521 gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc ccggtaaccg ttgccgcgcc
     2581 gcccatgtcc gacttcatcg agtccataaa cgcagtctgt ttgatgctgt agccgccgga
     2641 gtcaaaagtg atacctttac ctaccaggca cgcgtacacc ggtgcttctt tatcgccagt
     2701 tgggttgtaa tccagcgcca gcaataccgg agaacgttct gaaccgcgcc cgacagtgtg
     2761 cagccccata taaccttgct cacgcagatc ttcgcctttg gtgatccgat aagtcacacg
     2821 atcgcccgcg acgttgctga tcagatcaac agcacgctgt gccagttgcg atggtcccaa
     2881 ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg atgatcatca ggcggttatc
     2941 cagttcctgg cgctgggcat cgtccagatc cggccactcc actttacgcg tgcctttcgg
     3001 gcctttataa ccttgccaga atgcccagca gcgatccgca tcccagcctt caccgcttaa
     3061 ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc gcacgctgga tcagccccag
     3121 atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta ttaatgctgt aagttgcttt
     3181 ttctccccag cgcgcgtcgg caggttgggt agagagggta atcttcatcg cttctgtcat
     3241 tttagttatc cttattcgtt taacagcaaa acgggctacc cccaggcagc ccgttcattc
     3301 actcggcttc gtccagccag actaacaaaa tcgcttcgag gattttctcg ttggatgcct
     3361 gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg atgcatatcg gtgaatcgaa
     3421 ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat
     3481 cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt tgatagtgta
     3541 acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc gcctggcagc ttaaacggct
     3601 ttccggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg agctttccgg
     3661 cagtgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac aggatttttc
     3721 acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg tcgagaatgg tttcaccgct
     3781 attagcttcc agaacagcgc catcagggca gaggtcctga tgaggcaaaa taacaatctt
     3841 tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc gactggtcca
     3901 tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct tgttcgatgg
     3961 cgtcaacatc atcgccctgc gccacttcac tcaggtgagc ggcagcattg tcaatgacct
     4021 gacgctctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc agactttcca
     4081 gcacacgcgc cgcttctact ttttgttctg ccagcatacg ggcttttacg tcctgctcgg
     4141 catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga ccgtaagacg
     4201 gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc acgctcaaaa
     4261 gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accgccagcc ggtagcgccg
     4321 gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt tcgcgctcac
     4381 cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg aaatcctgag
     4441 cgcgggccac cggaatagtg gtattacgcg gaatcacttt ctccaccagg ccgcccatcg
     4501 tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg tctggcttgt
     4561 tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta tccgggtcga
     4621 tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc agcggcacgc
     4681 gagtagaacc gcccaccatc accacttcca gcacttcgtc agcttctaca cccgcgtctt
     4741 tcagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc agttcattga
     4801 attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat
     4861 cgctcagcgc gattttggct gcaatggtgg catccagcag ttcacgctga acgcggttat
     4921 cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc cgccagcaga tggtcgaaat
     4981 catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg ccgcgactta
     5041 agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg atcacgcctt
     5101 cctgaccgga atccagcccg taggcgatag ccgcagcagt cggttcgtta agtaagcgca
     5161 gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa
     5221 agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct tcagttgccc
     5281 gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagt agccccgccg
     5341 ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc agatgcggat
     5401 agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa ctaatggtgt
     5461 tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt
     5521 gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac gtttcggcct
     5581 gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata ccagccgcca
     5641 gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat aaggccatgt
     5701 ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc actgctccgc
     5761 agtttatcga gaaaacgcag cttacgcacc gtatccgccg ccgcgtccca cgcctcgttg
     5821 tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac acgtttgata
     5881 aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc ttcgcgcagc
     5941 tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct ggcgagatca
     6001 aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag cgtttgccag
     6061 gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgccag gcttccgctg
     6121 gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct cagtgcctgg
     6181 gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat aacattctca
     6241 gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg cgcaggcgac
     6301 cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat
     6361 ctttaacgtt cgggttggtg aatttaaacc cttcattcag gccttctttt acgaagtcca
     6421 gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc acgcctttgt
     6481 cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac
     6541 ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg cggttagcca
     6601 gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata accaaacctc
     6661 aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt gatcgcgtct
     6721 tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc agcaatatcg
     6781 gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc ggtgaccagg
     6841 gagctggaag cgatagcgga accgcagccg taagttttaa aacgcgcgtc ttcaatgata
     6901 ccttcatcgt tgactttaat ctgcaacttc attacgtcgc cacaggccgg tgcacccacc
     6961 atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac ccacgttacg cggattctcg
     7021 taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc cgataccgat
     7081 taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat ttcccacagc
     7141 ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt gtagtcgatc
     7201 tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc cagctcgtcg
     7261 ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa
     7321 cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc aacgtagttg
     7381 aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag gtaaacttct
     7441 tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg
     7501 gtcgccatct cttcttttgc gatgcgatag gcttcgccca ttccgacgat ctggtgaaca
     7561 ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg
     7621 cgtacacgcg gtttacgacg cacatacagc gcaccgatac ctttcgggcc atagattttg
     7681 tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgattgg cagtttgccc
     7741 acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat ttcgccgata
     7801 gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga aacaaggatg
     7861 gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga taataccgtt acgctgcggt
     7921 gccaggtagg tgacttcgaa accttcgcgc tccagctgac ggcaagtatc cagtaccgct
     7981 ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa gttggctgca
     8041 cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat ttcacgcgga
     8101 tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc ttcagcctgc
     8161 cagccgaaac ggtgagaacg agaggccggg ttaccaaagg ttccgtccat cgtcatgaac
     8221 tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta gtcgagataa
     8281 atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga atcaggctac
     8341 cggctggatg tacgaccgtg tttacggagt atttagcact ccggcctgat tctgaattct
     8401 ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg tgcgggtgcg tggcgcgtcg
     8461 tgagtatgct gacgaccaga cacatccagc acttcctggt tattaaccag ttcgcctaaa
     8521 gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc
     8581 aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc atctacagat
     8641 tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga
     8701 taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag acgggaaaac
     8761 agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc caacggtacc
     8821 gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata gcgcccttta
     8881 gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt tttttattgt
     8941 aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact
     9001 atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt atcgtggcgt
     9061 ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga cctacgattc tgtacattgt
     9121 aggcctgata agacgcgtta agcgtcgcat caggcaacca ctgttattcg gccttgttac
     9181 ctttattctg ctgctcaata gaagccagaa tcccgcgcag gatattcaat tcctggcttt
     9241 ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc ggatggtttt
     9301 cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa cgctccagat
     9361 catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccattt tcctgagttg
     9421 ccagccaggc catacgcact tcataggcaa taacctgaac cgccattgcc aggttcagcg
     9481 aactgtattc cgggtttgcc gcaatcgcga catgataatg gcatttctgc aactcttcat
     9541 tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac cggggtattt gccgcttcag
     9601 cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg ccacggcagc gtgcgggagc
     9661 gtgcgctggt gcccaccacc aggctacaac cggctaacgc ttcgtcgagc gtatcgacga
     9721 tgtgagcatt accaatcaca tcgctggccc ctgcagccag ggcaatcgcc tgggagtcgg
     9781 gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc atggcacggg
     9841 caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga atattttgca
     9901 gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg aagacagatg
     9961 ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag tgagagagac
    10021 cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt aatttagttg
    10081 ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt aacgatttcg
    10141 tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt aaatcttacc
    10201 cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat caggatgttc
    10261 aatgggttat cgatccactg gatggtacta ccaactttat caaacgtctg ccgcacttcg
    10321 cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta tacgatccta
    10381 tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc acagctgaac ggctaccgtc
    10441 tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc ttcccgttca
    10501 aagcaaaaca gtacgccact acctacatca acatcgtcgg caagctgttc aacgaatgtg
    10561 cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct gcgggtcgtg
    10621 ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc gagctgctgg
    10681 ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg tcataactat atgctgaccg
    10741 gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat actggcgaac atgcgtgacg
    10801 agttaagcga cgctctgaag cattaatgac tcaggcgggt gatatcactc acccgccctc
    10861 gcctttcagg cgctattccg aaatacttcc tcaccgcttt actttctttc gtcactctcc
    10921 caccatcttt ccccgattaa tggatgataa agaagtcgca aggaaaggtt atggcactgc
    10981 cagtgaacaa acgcgttccc aaaattctgt ttattctctt tgttgttgcc ttctgcgttt
    11041 atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa atttacggtc
    11101 ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact cgtctgcaag
    11161 gctggtttat tccttcttcg acgggccctg ctgacaacgc catcgcaacc atcattcatg
    11221 ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg ttacctgagc
    11281 gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa ggcactccgt
    11341 cccaggccgg attgctggac gatacgcaaa gcgccatcaa tgtggtgcgc catcgcagtg
    11401 atgtaaaccc acaacagctg gtgctgttcg ggcagagtat tggcggggcg aatattcttg
    11461 ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt gatcctcgac tccacatttg
    11521 cctcttatgc aaccatcgcc aaccagatga tccccggcag tggctactta cttgatgaga
    11581 gttacagcgg cgaaaattac atcgccagcg tcagcccgat cccgctttta ctcattcacg
    11641 gtaaagccga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc ctggcaaaag
    11701 agccaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt tccgatcgtc
    11761 acggcaatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg aatccgcaga
    11821 actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga catgctaatc
    11881 cactggtcag aacagtttaa aatgagaaaa attctgtgac gcttgccaac atttctgatg
    11941 attagcattc ccttcgccat ttccctgagc aaactttagc tattcttatc aattatgctt
    12001 atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc ggctccccag
    12061 cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccac catggcaacc
    12121 ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag cgaaacagga
    12181 cgggagatcc tgcgctatca tcaactcaca ctgacgactg gttatgacgg tagctaccgg
    12241 gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg acgtggtttc
    12301 cgtctgtgtc cgtcatttat taccagccaa ttcacccccg ccctgaagag tgaactgaag
    12361 cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt agtgaatctc
    12421 tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata ttcatttcct gtgtctgtat
    12481 ctgcaatatt gtttgctgca gcaccacgct ggaattacgc ctcagtttaa tccgctccaa
    12541 cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg acgccactgg
    12601 cagcgtcggg cgctccagca tgtaccgcct gatgagccac tgtttatggc attacttttt
    12661 tccatgttgc gggttcccga tccactgcgg gatgcgcatc agcgggacag acaattgcgt
    12721 cagtctatca aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg tttttatgat
    12781 gaacaggggt tatgtgatca gctttatatc cacctcgccc aggcgttaaa tcgcagtttg
    12841 tttgccatcg gtattgataa taccctgccg gaagagttcg ccagactgta tccacgcctg
    12901 gtacgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt ccatctttct
    12961 gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct ggctaatgca ggaaaacgat
    13021 ctgcatgaaa aacagattat tctactgacc gggaatgata gcgagcgaga agcgcagatt
    13081 gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaagcatat gtcggtaaag
    13141 gcatttttgc agacaggcgc tccgcgtggc gcggcactga ttattgcgcc ttataccatg
    13201 ccgttaccgc tcttttcacc accgctgatc tatacggacc tgacgctgac aacacatcaa
    13261 caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg ggcgcaaaaa
    13321 cattgccgga agcgccacca gcgccattac ccagaacacg ccgtggccca gatattgata
    13381 caggaaaccg gcgaaaacgg tcatgatagc gatactgccg cccatcgcga cggcagagta
    13441 aaccgcctgt aaacggatga cttcgctaac ctggcgagca gcaatataac gcatggcggc
    13501 caggtggcag accgtgaagg tgccgcaatg cagaatttgc accactatca accacggcaa
    13561 cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg agatcaacag
    13621 catatcgcga gcactacaac ggcggaaaag tttattactc agcgcaaaga taatgacttc
    13681 cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc cagctgcctg
    13741 ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata aacaaacgca
    13801 ggccagaaag cgccagttct ggcgaaccag cgccaaccac gcagaccaac cggtgctctc
    13861 ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg atgagaaagc cgagcagcat
    13921 ggatgccacg cccaacgtca acagcacgag aatcacccga taatcaaaca tagtgaccag
    13981 tttgcccgtc agcgccgagc caatgacaaa cgccaccgag ccccacagtc gcactttgcc
    14041 gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca acggtaccag
    14101 cggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta cgtgcgcccc
    14161 cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc agcacgcgca aggcggaaat
    14221 caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa aacgggcaac
    14281 cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc ctttaagcca
    14341 gacgctccag aaaggtaaaa aaatgccgta actaaaaaag tatgtgaaat agccgagcgc
    14401 caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt tagtctgggg
    14461 ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt cccgatgtct
    14521 gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg caaaaataat
    14581 gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga aatcgcgtaa
    14641 tgccagcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag gcacatttcc
    14701 ggcaatggga cgaaaaacaa cctgcccggt attaaaatta ttcatataac cgggtatcaa
    14761 agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg ttgccacctg
    14821 gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg gcgcaagcga
    14881 accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg cggcggtgat
    14941 ctctttttca tgtgctaaag ggtgatcaac cggtaacacc accactaatg gttcgtcaaa
    15001 aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc cgacgtcgag
    15061 ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca gctcaatcaa
    15121 ggtgtctggc tgtctgagac gaaacatcgg taatactttt ggcaataaat tcacttccgc
    15181 cgatggcaca aagccaatgg ttaattgtct gtcttcctga acaattttcc gcgcccgtaa
    15241 tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac attctcccgc
    15301 cgccgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga cacagttttc
    15361 aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt ccgccgcacg
    15421 ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta gttccatcac
    15481 cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt cacatattag
    15541 caaccaacca gcaacatcct tatggcacaa aaatagaagg tcaatacatc ttatctttca
    15601 ggattaaaaa atatgaccac accctcagat ttgaacattt accaactgat tgacacccaa
    15661 aacggtcggg tcactccgcg tatttatacc gacccggaca tttaccaact ggagcttgag
    15721 cgtattttcg gtcgttgctg gctatttctc gcccacgaaa gccagatccc aaaacccggt
    15781 gatttcttta acacctacat gggagaagat gcagttgtcg tggtgcgcca gaaagacggc
    15841 agcatcaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag ttatgcagat
    15901 tgtggcaaca ctcgcgcctt tacctgcccg tatcacggct ggtcttatgg cattaacggc
    15961 gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg taaatcccac
    16021 tgggggctaa acgaagttcc ttgtgtggag agttataaag gactgatttt tggcaactgg
    16081 gataccagcg caccgggcct gcatgattac ctgggtgaca ttgcctggta tctggatggc
    16141 atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa gtgggtgatc
    16201 aactgtaact ggaaattccc ggcagagcag ttcgccagtg accagtatca tgctctgttc
    16261 agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga taagcgcctc
    16321 ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct gcaatttggt
    16381 caggacggtc acggtagcgg tttcttcttt actgaaaaac cggatgctaa tgtctgggtc
    16441 gatggcgcag tttcaagcta ttaccgcgaa acctatgccg aagcagaaca acgtttaggt
    16501 gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat tccccacgct ttcatggcta
    16561 aacggcactg ccacactccg cgtctggcat ccgcgcggcc ctgatcaagt tgaagtgtgg
    16621 gcgttctgta ttactgacaa agccgcctcc gatgaagtta aagccgcttt tgaaaacagc
    16681 gccactcgtg cttttggtcc tgctggtttt ctcgagcagg atgactcgga gaactggtgt
    16741 gaaatccaga aattgcttaa aggccaccgc gcccgcaaca gcaaactgtg tctggaaatg
    16801 gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg gcattactaa ctatatcttt
    16861 tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag tagcgaaagc
    16921 tggcaggaag tgctcgataa aaccgccgct taccagcagg aggtgatgaa atgagtgcgc
    16981 aagtttcact ggagttacat caccgcatta gccagtttct ctttcacgaa gccagtctgc
    17041 tggatgactg gaaatttcgt gactggttgg cacagctcga cgaagagatt cgttacacca
    17101 tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa aggcgtccag ccgccgacga
    17161 cctggatttt caatgacacc aaagaccagc tggagcggcg aatcgcccgt ctggaaacgg
    17221 gcattgcctg ggcagaagag ccgccgtcac gcactcgtca cttaatcagc aactgccagg
    17281 taagcgaaac cgacatccca aacgtatttg ccgtgagggt gaattatcta ctttatcggg
    17341 cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa gttcgccgtc
    17401 tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa gcggtgatca
    17461 cttcccataa cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc ccgttgcgga
    17521 tgtgccggag ggtgaggctc tccggataga tacctcgccc gtcatcgcct tgttcaacgt
    17581 gggcggcgag ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt cgatgtcaga
    17641 agggtatctg gaagatgacg ccacggtgga gtgcccgcta cacgccgcca gtttttgcct
    17701 gaaaacgggg aaagcgttat gcctgcccgc caccgatccg ctcaccactt atccagtaca
    17761 cgttgaaggt ggtgacattt tcatcgactt accggaggcg cagccatgag cgatctgcat
    17821 aacgagtcca tttttattac cggcggcgga tcgggattag ggctggcgct ggtcgagcga
    17881 tttatcgaag aaggcgcgca agttgccacg ctggaactgt cggcggcaaa agtcgccagt
    17941 ctgcgtcagc gatttggcga acatattctg gcggtggaag gtaacgtgac ctgttatgcc
    18001 gattatcaac gcgcggtcga tcagatcctg actcgttccg gcaagctgga ttgttttatc
    18061 ggcaatgcag gcatctggga tcacaatgcc tcactggtta atactcccgc agagacgctc
    18121 gaaaccggct tccacgagct gtttaacgtc aacgtactcg gttacctgct gggcgcaaaa
    18181 gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga ttttcacact gtcaaatgcc
    18241 gcctggtatc ctggcggcgg tggcccgctg tacaccacca gtaaacatgc cgcaaccgga
    18301 cttattcgcc aactggctta tgaactggca ccgaaagtgc gggtgaatgg cgtcggcccg
    18361 tgtggtatgg ccagcgacct gcgcggccca caggcgctcg ggcaaagtga aacctcgata
    18421 atgcagtctc tgacgccgga gaaaattgcc gccattttac cgctgcaatt tttcccgcaa
    18481 ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa tcgcgcatta
    18541 agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg ccacgtagcg
    18601 gctgggctgg atctttaagg aagcacgatg aaagaaaaaa cgatcattat tgtcggtggc
    18661 gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc aagggttcac cggtgagctg
    18721 catctgtttt ccgatgagcg acatcttcct tatgaacgac ctccactctc gaaatccatg
    18781 ttgctggaag attccccgca gttacagcag gtgttacccg ctaactggtg gcaggaaaac
    18841 aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac acgagagtta
    18901 gtgttaacca acggcgaaag ctggcactgg gatcagcttt ttatagcaac cggcgcggca
    18961 gctcgaccgc tgccgttgct tgatgcactg ggagaacgct gctttaccct acaccatgcc
    19021 ggtgatgccg ccagactgcg agaagttctg cagcccgaac ggtcagtcgt gattgtcggt
    19081 gccggaacta ttggtctgga actggctgcc agcgccacgc agcgcagatg taaggtgaca
    19141 gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac caccgcccgt gcaacgctat
    19201 cttttacagc gccatcagca ggctggtgtg cgcattctgc tcaataatgc cattgaacat
    19261 gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg gggaaacgct tcaggctgat
    19321 gtggtgattt acggtattgg tatcagcgcc aacgagcaac tggctcgcga ggccaacctt
    19381 gatactgcca atggcattgt cattgatgag gcttgccgca cctgcgatcc cgcgatcttt
    19441 gccggtggcg atgtggcaat cactcgtctt gataatggtg cactacaccg ctgcgaaagc
    19501 tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg caatgttagg gctaccgcta
    19561 ccgctactgc cgccgccgtg gttctggagc gatcagtaca gtgataactt acagtttatt
    19621 ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc cggaaactca gaaggcgatt
    19681 tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtca cgctgaatca gggccgtgag
    19741 attcgcccaa ttcgcaaatg gatccagagc ggcaaaacgt ttgatgcgaa actgctgata
    19801 gatgagaaca tcgcgcttaa atcactgtaa tcaggataat tagcgaatat ctcaatgcct
    19861 ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat cacattatct tgcaaaggga
    19921 ttggttatga acacactacg ttattttgat tttggagctg cccgccccgt tttgttatta
    19981 attgcccgta tcgccgtggt cttaattttc attatttttg gttttcccaa aatgatgggc
    20041 tttgacggta cggtccaata tatggcctcg ttgggcgcgc caatgccgat gctggcagcg
    20101 attattgcgg tagttatgga agtgcccgcc gcgatattaa tcgtgcttgg ctttttcacc
    20161 cgtccgctgg cggtgctgtt tattttctac acgctgggta cggcggtgat tggtcaccat
    20221 tactgggata tgaccggcga tgcggttggg ccaaatatga ttaatttctg gaagaatgtc
    20281 agtatcgctg gcgcgttctt gctattggca attaccggac cgggggcaat ttctctcgat
    20341 cggcgttagg aaagatgccg gatgcggcgt gaacgcctta tccggcattt tataaattac
    20401 agccactcta cccgcaacga catctctgag gttgttgatt cccctggtgc tagcgcaatg
    20461 agatcacccc cttccgggcg atgatgatca tccggcgcat ggctcatcgg ttccagacag
    20521 aagaaatcaa acgcatatcc tttatcaaac gcagggtcgg aaacaaagat gaaataacac
    20581 ggtgcaggtg gcgtcgtttc catgatgatg gcataacctt cctgcggctg ctcgatgcgg
    20641 gcctgaccat tccatccggc aaaaccattg ttcacccact gacgcggcaa cggcgcgagc
    20701 tggttaaaat ccagttcctg cggtagctgc tcgcaaaact cacccgccag ccactgctcc
    20761 cgttccagcc agtaaccgct cgcctgcgcc tgaatccgcg tttgtggtga caacggaaaa
    20821 taaggatgcc agccggtacc aaatggcagc gtctctgccc cttgattggt gacagagagc
    20881 gtcaccgtca gcgtatccgc cgttaaatga aacgcctgac ttacccgata gtgatagaca
    20941 ccgctgcgat gttcatacac cagacacaaa ctatcatcgc tgtgcgagac acattgccac
    21001 tcgcccagcc agccatcgcc gtgtagatag tgcgcatccc actcaacatt cggttgcagt
    21061 tgatactcac gcccctgcca gacaaaacgg ttgccgctca cccggtttgc aaatggcacc
    21121 agcggaaaac atgaggcatc ggttgccaca ccgctttttt taccaggacg taagagcggc
    21181 gtcgtatcgc gccagaagcc ttcgattacg ccgccctggt cagaaacgtc cagctttagc
    21241 gacccgtggg ataaggtata gatggtcatc cgctaactcc ttaatccggg aagttaatca
    21301 caactttccc gcatttgccg ctcgccatca gcacataggc atcacctgcc tgttctagcg
    21361 agaagcgatg ggtaatggcg ttacgcggcc acagcttcca gtcagtcaaa tcgtgggcgc
    21421 atttttccat atggaacaga ctggtcaccc aggagccgat aatccgccgt tgatggtgca
    21481 tcagatcagc gctgacctcg aattccactt ttccggtttc accaatgtaa accacccgcc
    21541 cccagtcagc agtggattgc agtgccagca agcgacctgc ggcattaccg gaacaatcga
    21601 gcgcaacatc cgcgccaccg tgggtaagtt cagcaattat ctgcggcaga ccttcggtgg
    21661 ttgctaaata gccgtgatcc atccccccta actgttttgc catcgccaga cgttccggca
    21721 gcatatcaac gccgatgatc cgttttgcac cgcgaccttt cgccagcatc atcgccatca
    21781 tgccgaccgg ccccagaccg accaccagca cgttatcgct gccggaaact tcgccgcgca
    21841 aaatcccttc ataggccgtg ccaacgccgc aactgataaa cgcaccatct tcgtagctca
    21901 gcgcatccgg caggaggatc agatcttttt cttccgccag cagatattcg gcatgaccgc
    21961 cgtcacgctg ccagccgtaa gccgcttttc ctttgccagt acaagagata ggaaagccgc
    22021 ggcggcagtt cgggcaaaaa ccacagccag aaatgtgata caccagcacg cggtcgccct
    22081 ctttaaaatg gcggcagcct tgccccatcg ccacaatctg cccgcacggt tcatgaccgt
    22141 tgataaatcc ctggtataac ggtttatcgg gtgccgccgc cgtggcacgg tgttgatgat
    22201 agatatagtg gacatcgctt ccgcaaatcc cggaggattt cattttgatc agtacctggt
    22261 taatccccgg cgtcggcacc gcaacttccc gcagatcgac ggttgaattt cctagtaaat
    22321 aagcagccag catcgttttc ataaatccct cattaacaat acgattaatt ttcatccctg
    22381 ccgcacccgc gccaggggcg ttaaggttta gcgtttcgct ttactgcttc gctgggtcag
    22441 caagatattc gccagcaccg ccaccacgat gatgacgccg cgtaccacct gctggaaaaa
    22501 ggagttaata ccgagcagca ccagaccgtt accgattagc gtaatcacca gcacaccaag
    22561 caatgtaccg aacagggaac cgcgaccgcc ggaaagtgcc gtaccgccga ccacgaccgc
    22621 ggcgatgacg tcaaactcca gaccgtttgc ggcacctgcg ttaccggaac cgaggcgtgc
    22681 cgccaacaaa atgccggtca ccgccgctaa taatcccgaa agggtaaaga taagaatgcg
    22741 cactcgacga acgttaatac cgcacaactg cgccgccgtg gcattaccgc caacggcaaa
    22801 aaccgagcgc ccgaaggcgg tttttcggct aatgaacaca aacagcgcaa acaacaccat
    22861 catgatcagc gcggatactg gcacaccgag aaattgtccg cccagccagt ccagcacctc
    22921 gttttcgtca atcggcactg gcagtgcgtt ggtcataaac agccccattc cgcgcagagc
    22981 gctccacagc cccagtgtgg caacgaaact tggtacgtta aacacgccgc gcagcacccc
    23041 ggcgagcgtt cccatcagcg cgcctaacag caacaccagc agacaagcaa tcgccaacgg
    23101 aacttcaaat tgcagcaaaa atgccaggca caccgagaca aaagccacca tcggcccaac
    23161 gctgacatca atttcaccgg agataataat cagcgtcatc gcccaggcgg caatcccaat
    23221 ggtggcggca tcgcgcagca cgttcatctg gttattcaat gagataaagc caggcgcgtt
    23281 cagggagaag accagataaa gaatggcaat caccaccagc aaaccgatct cattaatatg
    23341 gcgactgaca aattgtttga gcgagacgct cttgccctgc ggcaatggta atgacgaagc
    23401 atacatcttc aggttcctcg gtaaaattca tcagtgtaca gacagaatgg cggacatcag
    23461 ctcatccaca ttgaccggcg agtgaaactc ctgcgagaac gtgccgtgct gtaataacag
    23521 gatgcggtca cacaccagcg gcaattcctc cacttcactg gagataaaca ccacgctttt
    23581 tccttcggca gccagctcac ggacaatacg gtaaatctgc tgtttggctt cgatatcgac
    23641 gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg gcatagaccc aacgaccgat
    23701 caccactttt tgctgattgc caccagaaag cgtgccgatg ggtgtttcgc tactggcagc
    23761 cttgaccgtc atccgctgca tcacctcttc ggtcaggcgg cggatggtgg accattgcag
    23821 cacaccgttg gtgctgattt tttgccgatt ggtcagcact gtattttcgt caacgcctag
    23881 ccagggaatg atccccgctt ctttgcggtt ttctggcgta tagccaatgc cgcgtttcag
    23941 catgtcgccg taatcggggc gcatgatttt ctcgccgttg ataacaattt cgccctgttc
    24001 atacgtctcc agcccaacaa tcgccttcag caattcactg cgccctgccc ccagcaggcc
    24061 agcaatgcca agcacttcgc cacgacgtag cgtaaagctg atatcctcca gcttgggctt
    24121 atggcgtaac gcacggactt ccagtacggc ctgatccaca atttcctgag gggcaaccgg
    24181 cgcaatatca acgtgatcac gccctagcat tagcgacaca atatgatgcg tggaggtgtt
    24241 ttcgagcatc acatcgcccg ccacctgacc atcgcgcata acggtggcat aggaggcaat
    24301 gcggcggatt tcttccatcc ggtggctgac ataaatcacc gccacgccca gtgccgacat
    24361 ctttttcacc gcactgatca ccagttcaac ttctgcactg gcaagcgagc tggtgggttc
    24421 atcaagaatg accacgcgcg gctcgccctt catcacccgt gcaatttcca ccagttgctt
    24481 ttgcgccggg cttagcgttg aaacaagttg ttcaggacta acgtcaacgc ccagcgcctg
    24541 taagcaacgt tgggcatcct gcgccatttg caggtaatca atcatgccgt tgcggcgagg
    24601 ccattgacca aggcagaggt tttctgccac cgtcagccct tccaccagac ttaattcctg
    24661 ataaaccgca cgaaccccca gttcagcggc acggcgagtc aacgtagctt cgtcaccttc
    24721 cagtcgcgtc tcgccaatcc agatatcacc gctatccggg cgttcgctgc cggtaagcat
    24781 tcgaatgaga gtcgatttgc ccgcgccgtt tttacctaac agcgcacgaa cttcgccttt
    24841 attgagcgtg aagttaacgt tatccagcgc aacgacgccg gggtaacgct tatttcctgc
    24901 caccactttt gctaccggga ctgcctctgt tgccgtgaac atagggacct ctgcgaatca
    24961 gcgattaggg cagaccatca acgtgcgttg ccagccactg tttaccatct tccgttttgg
    25021 tatagagatc gattggcacc tgaatcactt tttcaccgtc ggcttgttta ttgataacct
    25081 tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc actaccgctt
    25141 tcagcacctg attgttttcc agctcctgag caatttcggt tgtcatatcc gaaccgaaaa
    25201 cagcaatttt tccggcctga ttttgattac gtaccgcttt taccgcgccg agtgtcgcac
    25261 cgcccgactc ccccataatg gcgttgagat ccggcgtgga gataatcagt ttttcaccaa
    25321 cggaaatcgc tttatctaaa acagtccctt cctggttagc gacaatttgc gcgccgggaa
    25381 cgcgggattt taatacttct tcaaatcctt tacgtcgctg cacacaaact tcaaaggctt
    25441 cgcaattgat gacggcaatt ttcggctggt caattttatt ggcaataaaa taatcggcgg
    25501 cagcgttacc cagtttttta ccaaattcca gtggatcgcc gaccagatac gccgagacat
    25561 atttatcgac acccttttga ttaatacagg tgttgtagca aatcaccgga atacccgctt
    25621 cactggcgcg acgaacggta cggctactgc cattttcaga cactgccgat aaaataatgg
    25681 catcgacatt acgcgccacg agggtatcaa caaaggtact ttctttcgaa atatccccct
    25741 gggcgttagt ttcaattaac tgtacctgta ctgaagaatc ttttgccgca tcctgaacgc
    25801 cctgacgcac tccagcgtaa tatccctggg tatcaaggta tattgcacca atggtcattt
    25861 ctttatccgc agccctggca aatagtgcgc tacctaatag cgtagccatt aataatagat
    25921 tacgggttgt tctcattttt ttaggcatag tgcctcctgt agggttttta ttaacaacgg
    25981 cttattctaa ttattttgtg atgagcggca gcgcgtgccg ctcaaatatt acagttaata
    26041 gggtttagtg aacgtagtta aaaataaaag gcatcacagt ggtgaataat gccgctttcg
    26101 gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc gcaacctccc
    26161 cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta
    26221 aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc gcctcaatac
    26281 tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc tgtgcggttt
    26341 gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca accggctgat
    26401 cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac
    26461 gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag aaccagatgt
    26521 cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa tacagcgcgg
    26581 catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc caggcgcgta
    26641 ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc cacgggtaga
    26701 atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga tgccacaaat
    26761 tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta ttttccagac
    26821 gcgccagtcg tttctccggt gtggctccac ttaacttatc cagcaaatcc cgttcgaaaa
    26881 gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct
    26941 cataatcgtg tttcttattc cacgcatgga tcgctaaccc gcgccagccg tcggcaaact
    27001 ccggcgacat ctctacgcaa cgttgccata aggcaatggc tttgttgtag ctacgtttgt
    27061 tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata ctctccagcg
    27121 ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca tcaatggctt
    27181 ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg taaagcggca
    27241 gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc attccccagt
    27301 taatcagttg cccggcggtc atcagagcgt taacgtcgcg tccctgacac agaccgcgcc
    27361 actgcgcgag ggctgattcg ctacgaccat cgaaccagtt cagccaccac agagtggcgt
    27421 tcagcggata atcacgcagc agtttctcgc gctgcaaacg cgcgttgtcc tgacgaccac
    27481 ttaacaccag cagcagatta tgcaagcaaa gcacttcctg attggttggg caggcacgaa
    27541 gactttgttg gcaaaaatcc agacccgcgt cgaagttatc attacgcgcc gccagtcgtg
    27601 ccagaccata atagccgcct gctttactat tgccgctcca gaccgcacgc cagaaatcct
    27661 cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc aaacttgctt
    27721 gtccacactg cgggtttttg ttcagcgcat gtgcgcgttt cagcgcctga ctggcatacg
    27781 ccaccgcttg cgggaaatct gcgcggttat attccagcat cgccagcgcc aggttacagc
    27841 gataatccag cgggtccagc gccacgccgc gcaggtagta atcgaacggt gaacggctgg
    27901 cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta ctggtaatgt
    27961 cttgtgctgc cagtggcgct ttggcgacgt ccggcaacgg caactcctgc ggctgatgtt
    28021 cctgataact cagtacaata ttgccgtcgg catcggagag ttcaatggtc agtctgtcag
    28081 gattgatacc gtgcaacacg ccctggatgg cggtcgctgg tgtcagggcc acggcatcat
    28141 cgagcaacgc gttgcatttg ccgatttcgc ggatcgccag acggtatccg ttcaacggag
    28201 agatggcata cagcccccac tcaatccccc gctcactacg ctggagtttt atcaccgcat
    28261 cgcgggaggc attttgcacc atgcccagag aatgataagg caggaaatac tgctcgaaac
    28321 gcttctcttc gtaagcatca agccaggtaa aatcaggctg gttatcggca aaaataccgg
    28381 tcatcagttc gatatacggg ccgttattgt cggtcagact cttatcccac gcctggccaa
    28441 attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg ttggcaacgt
    28501 gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt
    28561 cggccatata tgaggttgga acaggcacat ttttatagcg agaaatgtcc actccggcgg
    28621 agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg gcccgtttgc
    28681 cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct tcaccccctt
    28741 tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc
    28801 ggctggcgat ttccagtgcc gcccggtcag ggcgcagggt gaaacctgtc atcacctgta
    28861 aaccatgcat cggctccgtt tcgccgaccc acaccgtctg tgcaccgtct tcatgggctt
    28921 cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact
    28981 caatcccgcc ggagatccac ggtcccagca tccccaccag cgcaggttta atgacttcat
    29041 tgtgatagac aaaatcgcgc tgtttcactt tatcccatgc gcgatgcacc cgaccgccca
    29101 gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc tgctaggatt
    29161 tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc gcgccggacg
    29221 atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc ccggtttcat
    29281 aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg ccctcaatat
    29341 taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt
    29401 tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag cctgcattaa
    29461 caatcaacag attctccacc ataacaaatg cgtgattctg gaactgctgt accggcaaaa
    29521 gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa
    29581 tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg aaagccagac
    29641 gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga cgctgtgcct
    29701 gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg
    29761 cgaatttgag tatttacaga ttgatgtacc gacgccgcag gcgctgctgt ccgaaatcga
    29821 aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc tggcgctggc
    29881 aatccacggg caggttgatc cggtgaccgg cgtatcgcaa accatgccgc aagcgccgtg
    29941 gacaacgcca gttgaggtga agtatctgct ggaagagaag ctgggcattc gggtgctggt
    30001 cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt cgcaggaacg
    30061 ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga ttaacgagca
    30121 aatttatcgc ggcagtttgt atggtagcgg acagattggt cacaccatcg ttaatccgga
    30181 tggcgtcgtc tgcgactgtg gacgttacgg cagcctggaa accgtcgcct cgttaagtgc
    30241 attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc agcttgagcc
    30301 tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac cgtggatcac
    30361 cagctgggtt gatcgctctg ccaatgccat tggtttgagt ctgtataact tcctcaacat
    30421 cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg agaactggct
    30481 taatactatt attcgccaga caggatttaa cccgttcgac cgcgacgaag gaccgagcgt
    30541 gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc tgggaattgg
    30601 ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg tagaaaggct
    30661 tcccgaagga agccttgatg atcataaacg aaaaattgcc tgatgcgcta cgcttatcag
    30721 gcctacacgg agattgcaat atattgaatt tgcaagattt tgtaggcctg agaaggtgtt
    30781 catgccgcat ccaacatgaa caacgagcac attgtcagca aatcaccgtt tcgcttatgc
    30841 gtaaaccggg taacgtgcgc agatgtcgag aactttacct ttgatgcgct cgataacggc
    30901 ttcatcattg atgctgtcca gcacgtcaca catccagcca gccagttctt tcgcttcggc
    30961 ttctttaaag ccgcgacggg taatcgccgg agtacctaca cgaataccgg aggtcacaaa
    31021 cgggctcttc ggatcgttcg gtacgctgtt tttgttgacg gtgatgttag cacggcccag
    31081 agcggcgtct gcttctttac cggtcaggtt tttatcaacc agatcaacca ggaacaggtg
    31141 gttatcagtg ccgccggaaa ccactttgta gccgcgctcg aggaacactt ctaccatcgc
    31201 tttagcgttt ttcgcgacct gctgctggta agttttgaac tcaggctcca tcgcttcttt
    31261 cagagcaacc gctttaccgg cgattacgtg catcaacgga ccgccctgac caccagggaa
    31321 aacggcagag ttcagttttt tgtacagctc ttcgctacca cctttcgcca agatcaggcc
    31381 gccgcgcgga cccgccaggg ttttgtgagt ggtggtagta acaacgtgag catgaggaac
    31441 cgggttcggg tagacgccag cagcaaccag gcccgcaacg tgcgccatat caacgaacag
    31501 ataagcaccg atgctgtcag cgatttcacg cattttcgcc cagtcaacca cgccggaata
    31561 tgcagagaaa ccaccgataa tcattttcgg cttgtgttct ttggcttgtt tttccagatc
    31621 ggcgtaatcg atatgaccgg tagcatcgat accgtaagga acgatgttgt acagtttacc
    31681 ggagaagtta accggagaac cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag
    31741 aacggtatca cctggttcca gcagcgcggt gtagaccgca aagttagcct gggagccgga
    31801 gtgcggctgg acgttagcgt agtcagcgcc gaacagttct ttcgcacgat cgatcgccag
    31861 ttgttcaacg atatcaacat actcgcaacc gccgtagtag cgtttgcccg gataaccttc
    31921 agcatatttg ttggtcagct gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt
    31981 ttcggaggcg atcagttcga tgtgctcttc ctgacgtact ttttcctgct ccatagcctg
    32041 ccacagttcg gcatcataat cggcaatgtt catttcacgc tttaacatcc gcatctcctg
    32101 actcagctaa caataaaatt tttggccttt ataggcggtc ctgttggaca acggcgaaca
    32161 gtataaccga atcattgtgc gataacaggt cttgacaaag gaatttacgc aaacgattac
    32221 cttcaggcta cgcaaggctt tggagaataa agagcttgca accggaaacg gatttctttt
    32281 caggtttgtg atgcaaattt ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc
    32341 atttacattg cagggctatt ttttataaga tgcatttgag atacatcaat taagatgcaa
    32401 aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt
    32461 tactggtgga aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata
    32521 acccagaact caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag
    32581 ccctgtttaa cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc
    32641 cagcggtaga aaaaatcgcg cagaagcaca ccagcttcca gatcaaaccg gaacagtaca
    32701 acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag
    32761 tgctggacgc gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg
    32821 cggaaatcta taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc
    32881 gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg
    32941 acggtggcgc agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg
    33001 aaggtttccc gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag
    33061 gctatcgtat cgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc
    33121 acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg
    33181 tcgcagatga cacaccagtg acgttaatct ctaccggtgt tggtcaaacg ccaatgctgg
    33241 caatgctcga cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg
    33301 cagaaaatgg cgaagttcac gcctttgctg atgaagttaa ggaactgggg cagtcactgc
    33361 cgcgctttac cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc
    33421 agtttgatag cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga
    33481 caatgcagtt ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag
    33541 tggatctggg cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc
    33601 tgtaatttga tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg
    33661 cgtcgtcctc ttcaccggta cggatgcgaa tgacccgagc cacgtcaaag acgaagattt
    33721 taccgtcacc gattttgccg gtttgcgccg tgcgaataat ggtatcgaca caggtatcga
    33781 caatgtcgtc cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact
    33841 ccgcgccgcg gtacagctcg gtatggcctt tctggcgacc aaagcctttc acttcggtca
    33901 ccgtcatgcc ggtaataccg acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg
    33961 gttttataat cgcatcaatc tttttcatgc tattccttga aaaggttgcc tgtcttttga
    34021 tctgctaaac gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt
    34081 gtcgggaaag cagtttataa aattctgtcc ggttgcgccc cgccattcta gccgcgtggg
    34141 tgacgttgcc tttggtgatt tgcagcagct tacgcaaata gttgagttca aaatgattac
    34201 gtgcctcaac aaaggttggc agcgccgtat tttcaccctc cagcgcctgc tccaccagcg
    34261 catcactaat caccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact
    34321 ggcgcacatt acccggccag ctcgcggtca tcaggcgttt catcgcatcg gtagagaacg
    34381 cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagtagcg
    34441 gaatgtcttc tgtgcgctcc gccagtgccg gaattttcag gctgacaacg ttgaggcggt
    34501 aatagagatc ttcgcggaat tccccgcgcg ccatcgcttt tgacagatca cggtgagtgg
    34561 cagaaataat ccgcacattg atatcaatat cgcggttact gcccagcggg cgcactttac
    34621 gctcctgcaa cacgcgcagc agtttgacct gtaacggcgc aggcatatcg ccaatttcat
    34681 cgagaaatag cgttccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc
    34741 cagtaaacgc gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg
    34801 cgccacagtt aatagcaata aatggtttgc tgttgcgcgg gctggcgttg tggatagcct
    34861 gggcgaaaat ctctttcccg gtgccgctct gaccgttaat caaaacgctg acgtctgatt
    34921 gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaattg
    34981 cctcgcgcca gcgttcatcg gtggctggcg cggattgctc cagcgcatcg tcaattgcct
    35041 gatatagcgc atctttatcg acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg
    35101 cagcaacggc atcggggata gaaccatgcg cagtaagaat aattactggc attcccggct
    35161 gcactttctg gatttcagca aatagctgca taccgtccat ttcatccatc cgcaggtcgc
    35221 tgataactaa atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg
    35281 ccgtgaccac actgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg
    35341 gatcgtcatc gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg
    35401 gtggatggcg cgggcttttc actctcgtgc ggcgtatccg gcgagaaatt tccagccggt
    35461 ttgcgggtcg agagctggcg ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt
    35521 tccagttgct gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg
    35581 gactgctgga gtttactgta acgttggcgc tcttccgcca gttgcagttg cagcgcctga
    35641 ccgtcgcgcc agagttgata cagtgggcga acctgtgccg ggatctcggt acttaacgct
    35701 tcaatacgcg cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa
    35761 atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc
    35821 gactgcgcag gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg
    35881 gtttcggttg atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca
    35941 gccagttgat aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga
    36001 ttctgcacgc aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggggc
    36061 aataatcgtt gaaaaatgtg tcgcatattc accagactta aagcctatcc cagtgggcgt
    36121 aattgttgca gacagtctgg acatggacag cgcggagaaa ccggggcgta catatcgtac
    36181 gtgaagattt cgagcactac ccggggccga actgacaaat aaaatagcct gataggatag
    36241 gctgttagca ttatttcgtg tttttcgacg acggtaattc aatgcggaaa caaacgtctt
    36301 gcccgctctc gtcgaccaga tacagttccc cttgcatacg gcgaatacaa tccctggcaa
    36361 tgcttaatcc cagaccgctg cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa
    36421 aggggtcgaa gatcatggcg cgttcctctt gcggaatggg cgtgcctgta ttgatgacat
    36481 caatataaac ccgcgcacca tgtaaactgc tgcgaaggca aatgttaccg gattcagccc
    36541 cgtagtgcac cgcattggag taaagattat ccagtacgct catcagcagc attggctccg
    36601 ccaggcaagc tgttgctttg agatcgacgt cggtatgcat cattttagcc cgtgcgggca
    36661 ggctatgagc agaaaccact gtctccacca gcggtgctaa ttcaacattc tccagttcca
    36721 ccgcactgtc cgcctgttta cggttgtaat caagcagttg ttcgatcagt ttttgcaaat
    36781 tgcagctgct gctatcaaga atgctcacca cctctttttg ctctggcgta agcggcccga
    36841 caacctgatc agccagtaat tcagtgccct cgcgcatact cgccagtggc gtttttaatt
    36901 catgagataa atgtcttaaa aattgatggc gttgggattc cagccatgac aggcgctcac
    36961 ttaaccaaag aatacgttgc ccaaccgagc gtaactcgct cggtccacag aacgaaacgc
    37021 tattgcccag agaacgccct tcccccagac ggttgatcat gcgctcgata tttttcaccg
    37081 gcccgataat catccgcgtg aaaagtagca ccatcaccag actcaccaga aatagcacca
    37141 gcgattgcca accaaaatat tgcccacgtt cggcgatttc acgctgaagt tgctgcccac
    37201 gagagaacac cactgtgcgc gtggcctgca ccatttcggt attggcactg gcaaaggctt
    37261 caagacgcgc ggcggcggca gcatcgggac cactgttgtt acactgaagt tgggccagat
    37321 tgttcaaatc ctgacgcaat gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg
    37381 catcgagcat ttcgctgtaa cgcttgcgct ggctttgata aaccttcgca agcgtagggt
    37441 cgtccagcac gcaatactga cggtagctac gctccatctc cagcgccgcg ttggtcatcg
    37501 cttcactacg ccgggcatca ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa
    37561 gcgcattcag gctttgccag gcttgccatg ccagcaccaa caggggtagt agaattagca
    37621 aaaatgccag cattaccagt tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg
    37681 tgttactctc gtcagacgcg aatagcctga tgctaaccga ggggaagttc agatacaaca
    37741 aagccgggaa ttacccggct ttgttatgga ataaggcggt gcctaactcg acgtttcgcc
    37801 cgatggttga tatagctacg ctgatatcag aagttggacg gcaggcacct tgttgtgcgt
    37861 cattcgaatt ttatgtagca cgtcccgaag gggctgacat aagtcggtga atgagccact
    37921 gcttactatt atgcagtaac tgtgccaata aagaaaatag tttggtaacg tactgattat
    37981 acgttgcttt gagggattta tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc
    38041 gctaaaaaga gacaacttaa gataaactta ttagattaca ataaaatcaa tcatttaagt
    38101 gtcgccagaa agcgacacgg taaaccaccc attgtcgtga tttacagaca caaaaaagct
    38161 cccggagttg ggagcttatg atagtggttg gtgcttaatg ccggatgcgg cgtaaatgcc
    38221 gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac gcgcattgcg
    38281 gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg agttgctgac
    38341 agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac tttcagtcgt
    38401 aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt cagtgacttt
    38461 gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg
    38521 cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg gcatttgcga
    38581 gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca ggctgaaacg
    38641 cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac tccccgggat
    38701 cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg acagcgtttg
    38761 cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggatagactt
    38821 cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc acgcgaccag
    38881 ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat gcacgtcgat
    38941 agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt tcacgccctg
    39001 ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg ctgccacatc
    39061 ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt tagatttcgc
    39121 ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt
    39181 ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct gcccgttggc
    39241 ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc aatccgcaag
    39301 cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatcac
    39361 cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag aggcgatatc
    39421 cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca gcaggcctcc
    39481 atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct gaatcgcgtc
    39541 atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc caagctgacg
    39601 ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag
    39661 cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta catcttccac
    39721 gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc
    39781 ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag
    39841 cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg catcttcacc
    39901 agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat
    39961 ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa
    40021 atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc
    40081 gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc ccaccatctg
    40141 atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga aagttttctc
    40201 cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg
    40261 gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt
    40321 accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag
    40381 agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt
    40441 acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg
    40501 ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg
    40561 cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg cgttagaaag
    40621 accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa gctgccagca
    40681 acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct gtacggaagc
    40741 aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc cgccgccaag
    40801 accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt tgatctcgcc
    40861 tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg
    40921 ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag tacggaagta
    40981 gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca gcgggccttc
    41041 ggtcatgatg tccagcgcag taacaatgcg ctcaggctta ccgaaatctt cttcccacgg
    41101 ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac cagctttcgg
    41161 cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc
    41221 gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcaggatgtg
    41281 cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac
    41341 ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt gatctggcgt
    41401 ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt caccgtcgat
    41461 aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct gagcgaacat
    41521 atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc
    41581 aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca gcgcctgacg
    41641 gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg caaacaactg
    41701 ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt
    41761 aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg
    41821 ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg ctttcgaaga
    41881 ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg
    41941 gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt
    42001 taacgacgca ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag
    42061 cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga
    42121 accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg
    42181 ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat
    42241 aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg
    42301 gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt
    42361 tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat
    42421 tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc
    42481 tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg
    42541 agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctagat
    42601 tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg
    42661 cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct gctggcggca
    42721 ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac ctattattcc
    42781 gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg
    42841 acggcggagc aactcaccgt tgcaccgggt catgtggtgg ttaacgatct ccagaccctg
    42901 aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa
    42961 ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc
    43021 agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac cgatgaacaa
    43081 ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac
    43141 ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa atacctgggg
    43201 catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta
    43261 ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg
    43321 gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc gacgggtgtg
    43381 cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc
    43441 gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat gagtaaagtg
    43501 ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta caatatgggc
    43561 tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt
    43621 tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg
    43681 acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaagtat
    43741 cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga agcggcgatg
    43801 caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga gaaatttcct
    43861 tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc ctctctgctg
    43921 ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg cgcttttttc
    43981 tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca
    44041 tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg atgcagcaca
    44101 tccattaaag atcccacagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca
    44161 atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt gacatacaac
    44221 gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag ggccatgatt
    44281 tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc gccgattacc
    44341 cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca ggcacgtttc
    44401 gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc agacaaaaag
    44461 aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc
    44521 agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa ataaagcagc
    44581 ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg
    44641 cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac ccaaccacca
    44701 tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa
    44761 taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc agcatcggaa
    44821 cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcgcga
    44881 aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg
    44941 cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca
    45001 atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg gcgacgcagt
    45061 aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa
    45121 aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat
    45181 taattttcgc tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc
    45241 gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt
    45301 cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg
    45361 aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga taaaaaactg
    45421 gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc
    45481 aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa
    45541 ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc
    45601 tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa
    45661 ctgattaaag aaaatactgc cgctatgtac gcaacgctaa acgttaatag tgaagagaaa
    45721 ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac cggtattggc
    45781 gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct
    45841 gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat
    45901 ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc ggcagatgag
    45961 atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa tgccctgcaa
    46021 cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca
    46081 atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc gctgattcag
    46141 caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg
    46201 gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct
    46261 tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa tcaaataaaa
    46321 acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt ggatttatgg
    46381 tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt taacgtcctc
    46441 cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa cattaacttt
    46501 tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat aaccctggct
    46561 ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac cttctggcga
    46621 gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga aagtttggga
    46681 cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt tgttcgttca
    46741 acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa aaagcgtccc
    46801 attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc tcgttcgcgc
    46861 aactcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc cagcttccag
    46921 acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc cggtctttcg
    46981 gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca gttcaacgca
    47041 ttacgttgta gatcggtatt ctggtcattt gttgatacgc gtacatagcc aataagcatg
    47101 gt
//
LOCUS       DAJTUN010000016        44541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTUN010000016 DAJTUN010000000
VERSION     DAJTUN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.016"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(4..195)
                     /locus_tag="OO470_000865"
     CDS             complement(4..195)
                     /locus_tag="OO470_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250203.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(192..614)
                     /locus_tag="OO470_000866"
     CDS             complement(192..614)
                     /locus_tag="OO470_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HCR6250204.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
     gene            complement(661..1086)
                     /locus_tag="OO470_000867"
     CDS             complement(661..1086)
                     /locus_tag="OO470_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6250205.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1502..2272)
                     /locus_tag="OO470_000868"
     CDS             complement(1502..2272)
                     /locus_tag="OO470_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250206.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPSRRWFDRDILREVVLEARTEIRSMADALAKIRK"
     gene            complement(2317..2751)
                     /locus_tag="OO470_000869"
     CDS             complement(2317..2751)
                     /locus_tag="OO470_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HCR6250207.1"
                     /translation="MYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            complement(2765..2986)
                     /locus_tag="OO470_000870"
     CDS             complement(2765..2986)
                     /locus_tag="OO470_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250208.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(2987..3670)
                     /locus_tag="OO470_000871"
     CDS             complement(2987..3670)
                     /locus_tag="OO470_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6250209.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(3746..4058)
                     /locus_tag="OO470_000872"
                     /pseudo
     CDS             complement(3746..4058)
                     /locus_tag="OO470_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4055..4957)
                     /locus_tag="OO470_000873"
     CDS             complement(4055..4957)
                     /locus_tag="OO470_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6250210.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVLSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIIDNVAHYGG"
     gene            5375..5623
                     /locus_tag="OO470_000874"
     CDS             5375..5623
                     /locus_tag="OO470_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6250211.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5620..6057
                     /gene="umuD"
                     /locus_tag="OO470_000875"
     CDS             5620..6057
                     /gene="umuD"
                     /locus_tag="OO470_000875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6250212.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            6057..7331
                     /locus_tag="OO470_000876"
     CDS             6057..7331
                     /locus_tag="OO470_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR6250213.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(7333..7749)
                     /locus_tag="OO470_000877"
     CDS             complement(7333..7749)
                     /locus_tag="OO470_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6250214.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(7742..8722)
                     /locus_tag="OO470_000878"
     CDS             complement(7742..8722)
                     /locus_tag="OO470_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6250215.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            complement(9136..9444)
                     /locus_tag="OO470_000879"
     CDS             complement(9136..9444)
                     /locus_tag="OO470_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HCR6250216.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(9531..10175)
                     /locus_tag="OO470_000880"
     CDS             complement(9531..10175)
                     /locus_tag="OO470_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR6250217.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(10355..11134)
                     /locus_tag="OO470_000881"
     CDS             complement(10355..11134)
                     /locus_tag="OO470_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCR6250218.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPVGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(11136..11549)
                     /locus_tag="OO470_000882"
     CDS             complement(11136..11549)
                     /locus_tag="OO470_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250219.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            12118..12372
                     /locus_tag="OO470_000883"
     CDS             12118..12372
                     /locus_tag="OO470_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250220.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDSQSARCLNELRASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
     gene            12372..12962
                     /locus_tag="OO470_000884"
     CDS             12372..12962
                     /locus_tag="OO470_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250221.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            13175..14227
                     /locus_tag="OO470_000885"
     CDS             13175..14227
                     /locus_tag="OO470_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250222.1"
                     /translation="MKDTITPLNYTKEAIKDLSVMEKGLRSNAPLTSYIMRQVIDMLD
                     SSVKFILPNCCDIIEPEEYRQTHFDLARLPYPVVTFEIPWFKDNVETQIGDFNISPSS
                     RRIALCWEARQSFEPIPGCNSILNTYIDGGVFILPVSWSDDLKIWILGVGGMFFPYNN
                     KLTKYEPDRTLPASRLVIDTLKENGVAKYNAAHFKAEPFITSMEFKDDLIKQVGSIER
                     LYAQIIMDTRDELQAFIQACSVLNCENVCPVTLSTKPERKFINGRKVQPPEKNKRPSY
                     TYKVLQLSETKVQSNHTGTGKSGGTKRMHLRRGHIRRKNNKLIWIRPAMINANSRAGI
                     VDKDYQINIRKEENKP"
     gene            14403..15005
                     /locus_tag="OO470_000886"
     CDS             14403..15005
                     /locus_tag="OO470_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCR6250223.1"
                     /translation="MGKYQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            15015..15250
                     /locus_tag="OO470_000887"
                     /pseudo
     CDS             15015..15250
                     /locus_tag="OO470_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15250..15516
                     /locus_tag="OO470_000888"
     CDS             15250..15516
                     /locus_tag="OO470_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250224.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(15707..15988)
                     /locus_tag="OO470_000889"
     CDS             complement(15707..15988)
                     /locus_tag="OO470_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250225.1"
                     /translation="MTMNEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(16098..16373)
                     /locus_tag="OO470_000890"
     CDS             complement(16098..16373)
                     /locus_tag="OO470_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6250226.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(16373..16657)
                     /locus_tag="OO470_000891"
     CDS             complement(16373..16657)
                     /locus_tag="OO470_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6250227.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(17556..18434)
                     /gene="repA"
                     /locus_tag="OO470_000892"
     CDS             complement(17556..18434)
                     /gene="repA"
                     /locus_tag="OO470_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6250228.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASPLV"
     gene            19137..19778
                     /locus_tag="OO470_000893"
     CDS             19137..19778
                     /locus_tag="OO470_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCR6250229.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            19919..20581
                     /locus_tag="OO470_000894"
     CDS             19919..20581
                     /locus_tag="OO470_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HCR6250230.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            20848..21468
                     /locus_tag="OO470_000895"
     CDS             20848..21468
                     /locus_tag="OO470_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6250231.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            21495..23189
                     /locus_tag="OO470_000896"
     CDS             21495..23189
                     /locus_tag="OO470_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6250232.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            23195..23512
                     /locus_tag="OO470_000897"
     CDS             23195..23512
                     /locus_tag="OO470_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HCR6250233.1"
                     /translation="MPEFFRHLFHKECPHGIFWFKRGLKMSAEKFRVVIYTNTCEKKV
                     SYIPATEDINIAIMPFQTADNGLIIVCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNR
                     VQL"
     gene            23628..23963
                     /locus_tag="OO470_000898"
     CDS             23628..23963
                     /locus_tag="OO470_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250234.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            24190..25260
                     /locus_tag="OO470_000899"
     CDS             24190..25260
                     /locus_tag="OO470_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HCR6250235.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSAATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            25264..25701
                     /gene="pilM"
                     /locus_tag="OO470_000900"
     CDS             25264..25701
                     /gene="pilM"
                     /locus_tag="OO470_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HCR6250236.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            25733..27352
                     /locus_tag="OO470_000901"
     CDS             25733..27352
                     /locus_tag="OO470_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HCR6250237.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISENCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            27373..28668
                     /gene="pilO2"
                     /locus_tag="OO470_000902"
     CDS             27373..28668
                     /gene="pilO2"
                     /locus_tag="OO470_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HCR6250238.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            28658..29116
                     /gene="pilP"
                     /locus_tag="OO470_000903"
     CDS             28658..29116
                     /gene="pilP"
                     /locus_tag="OO470_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HCR6250239.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            29220..30728
                     /gene="tadA"
                     /locus_tag="OO470_000904"
     CDS             29220..30728
                     /gene="tadA"
                     /locus_tag="OO470_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR6250240.1"
                     /translation="MLAPADQPPDDILEYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            30730..31824
                     /locus_tag="OO470_000905"
     CDS             30730..31824
                     /locus_tag="OO470_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCR6250241.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            31886..32422
                     /locus_tag="OO470_000906"
     CDS             31886..32422
                     /locus_tag="OO470_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6250242.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            32467..32952
                     /locus_tag="OO470_000907"
     CDS             32467..32952
                     /locus_tag="OO470_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6250243.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDSGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            32968..33594
                     /locus_tag="OO470_000908"
     CDS             32968..33594
                     /locus_tag="OO470_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6250244.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            33612..34973
                     /gene="pilV"
                     /locus_tag="OO470_000909"
     CDS             33612..34973
                     /gene="pilV"
                     /locus_tag="OO470_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCR6250245.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGKLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNIN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            35446..36267
                     /locus_tag="OO470_000910"
     CDS             35446..36267
                     /locus_tag="OO470_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR6250246.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PVRMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            36369..37571
                     /locus_tag="OO470_000911"
     CDS             36369..37571
                     /locus_tag="OO470_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCR6250247.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            37675..38133
                     /locus_tag="OO470_000912"
     CDS             37675..38133
                     /locus_tag="OO470_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HCR6250248.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWQAVLSQSAGRLHLSYMPTEKK"
     gene            38130..38966
                     /locus_tag="OO470_000913"
     CDS             38130..38966
                     /locus_tag="OO470_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR6250249.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            38950..40098
                     /gene="traJ"
                     /locus_tag="OO470_000914"
     CDS             38950..40098
                     /gene="traJ"
                     /locus_tag="OO470_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HCR6250250.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            40095..40385
                     /gene="icmT"
                     /locus_tag="OO470_000915"
     CDS             40095..40385
                     /gene="icmT"
                     /locus_tag="OO470_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HCR6250251.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            40449..44510
                     /locus_tag="OO470_000916"
     CDS             40449..44510
                     /locus_tag="OO470_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6250252.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQVTTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     AGNQSTQAEEHPRSEDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTAETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKLRKEKEMEHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEHPAAPSSAPEKAPVSAQPQQPDT
                     PVEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
BASE COUNT        11310 a        12041 c        11215 g         9975 t
ORIGIN      
        1 gtatcacctg tttctgctgc cctgaaggat gtagtgaccg cccaggaaaa ccgcctgctg
       61 attcgccaca aaatcgcaga tttcatcaac aatctgcgcg gtggtgagcg gtttctgccc
      121 catttcacga cgccgcgcat taatcacggc cctgtaatta cgcagaattt cgcgggtttc
      181 tgtgctgata ttcatgacat cgtctccccg tccagcgcct gcaacacaac gtccattgct
      241 tcgagttcct ccctgacgaa tgcgtcaccg tcgcccccgt tctcactccc gacggcatac
      301 aggcgcttca tttcactacc tgccagcgcc atcacttttt ccagcatcac cgccgggact
      361 gtcacatgac ggctggcgcg cttttcttcc atcagttcat tgacaatatc ggaaacaact
      421 ctgtcacatg cgccatatac aaaagcatcg tccagccgct gcatgacggt ttccagctca
      481 tcgtctgaca ggttccagtc ctgggctatg tgacgcacat ccccacgggt cagcaccacg
      541 gcaaaaacgc attcactgtc cggcacgtct gtatccagta catcgcggat gatttcttta
      601 acagtacagt acatgttctc gtctccttta caggggcggc attgccgccc cgtccgttaa
      661 tcagtcaagt gccgcgtaaa tcgcgttgca ttcggggtga aattcgatgt ggctccacaa
      721 ctgcgcatca cgcatcctgt acaggtgtgt caaccctgca tccccgctgt cgtggtacag
      781 ccacaactgg cggttaatca caagggaggt aaggatgatg cctgcggcat cagcgctcac
      841 ggtacggtca aaccagtttg caccgttcac catgtggaaa cggtcgccat ccggcatcat
      901 gtaaccgcca ccgtccggca ggcggatgaa gtgccagaat ccgccttcat attccggcat
      961 catttttttc gccagcgcgt atacagacgc ttcggcatac agaaaatcca ggccaaacag
     1021 gtaaggcaga aaagacagac ggtcgcactc ttcaacgttc agagtgacag gtttcgcata
     1081 ttgcatgatg ttttcctccg ttgttcacat attccgcgac accgggcggt gccgtgcctc
     1141 catctttgcc gggcctgcag ggttgcaagg gcgcaaggga agccgcagcc tgacgcgcac
     1201 ggctgacggg agttgaaggg tgagaccgcg aaatctgagc gggtgaacga ccggaaaatc
     1261 acggcagacg ggaaggaggg accgggtgac cgcagcggtt caccttacct tgcaacccgc
     1321 ccgcagaagc ccggctacga tggaggcacg gcacagcccg cacacggcgc tgaggccaca
     1381 taactgaaaa cccacaaggc catgccccgc atggccgggt aaagcggagc ctgcggcgac
     1441 atccttttgc tctttccggg gaccggctgg aaaaggcgga gatttttccc cgccagcgtc
     1501 atcatttccg gattttcgcc agtgcatccg ccatgctgcg gatttcagtc cgggcttcca
     1561 ggaccacctc ccgcagaata tcccggtcaa accagcgacg ggacggttca tcaggtatac
     1621 gcagcacatc gcaggccagc ccactggaca ggatatgctc cacacactca ctgaccagat
     1681 acatttccac cgacgaaaaa cgagcgcccc ggttcaggat ggcatcgagc accacgggca
     1741 ggttttcgtc gtcttcatcg agccagcagg ggtgatcgct ttccttcaca accaggcgaa
     1801 agcggcgcgt ctggcgggtc agcaacgtct gaccggttgt ggtcagcgcc gccctgatac
     1861 gccgcgcatc ttctgccacc cggcaactga cactgcgtac cgcatcgtgc accgcatcaa
     1921 gaactgccct gatttcgtct tcggtcagcc attctccggc atacgggaaa gacacctgcg
     1981 ttccgtctgc cggaagtacc ggcagcgact gccactgact gccggacaga acaagttccg
     2041 ccccggttcc ggtcagtttc tgaatggcag aggccaggag cggcggcagt acgccgccgt
     2101 gacggttgat aagtaacgtc gccagccggg gcgcatccag cagaacataa atgctgatcc
     2161 agcccggata cttcgggtta cgctggtcaa catccgtctc ttccggccag ccctgcgcca
     2221 gcagcaggtt acgggcgtgg cgacaaatca gtgcattcag cgtttcgttc atgattcatg
     2281 gtctccctga atcgccgccc gcaggcggcg ataacatcag tcgttttcgc gtgtgttgtt
     2341 tttcagcagg gcgcgggcct gattaaccac cgcctgtcgg cgggtgacgc attccgggac
     2401 ggcgaggccg ttatcacggg cggcccactc acgtttccac agtgcctgtt cggcggtctg
     2461 aatcagggac gccagcagtt cagcagggac ggtcacctgg cggttttctc tcacgttatt
     2521 gatgatctcc atcgccgcac cggaagaaat accggattca gtccgctggt cttccgggat
     2581 gtcctccagg cgcgccagga cagtcctgat ttcatgatcg gacagggaaa tatccattcc
     2641 gtcagcgagg gcctgaattt cttccgggga ccagataacc agcatcagcg gcgtatctcc
     2701 cggatacttt gctgccagcg cccggcagag cgtttcgcat gttccgtaca ttctcgtctc
     2761 cttctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatacgct tccatgaaca
     2821 ggcgattggc gcgatacagc gtctggcgtg tcaggcgttc aacaagcaca tcggaatcaa
     2881 agcgacagcg atgctccata tcgttcagcc tggcccgaag gtcacacatg gtgttggcca
     2941 cctccgccac gtcggcgcga agtttttcgt gtccggcata gttcatttac gcagcctccg
     3001 gttcaaacca gttatcattc gcggccccct gctgcatggc ccgttgcacg gcggcaaggc
     3061 gttgctgccc ggcacggtga tactgctcaa gcagctcgat accgatatac cggcgtccgg
     3121 actggagggc ggcgacgcag gttgagccgc tgcctgcaaa cgggtccagc acaattgcgt
     3181 tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt tccgtcgggt
     3241 gatgacgatt gccagaatat ttccagccca gcacgtccgg cagcgggttt tgtggcagac
     3301 gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca tatgcggcct
     3361 tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc
     3421 aggcggccat aaagcgatcg acgcggttcc agccgtagaa gctcaccatc agcgcgtctt
     3481 ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatcg gttttatcgc
     3541 cggcaatggt acgcccctga cggtcacgaa aaccgacgag gtacggcggg tcggtgagga
     3601 tgaaatcaat ggcgttatca gggatacggg ccataacatc gatgcagtta ccgaggacaa
     3661 aacgggacat accaaactcc ttctcttaca gagggcaccg ggagggcacc cctgtggtgc
     3721 ccgttccccg ctgcctgtgg gctgttcacc gcgtccggta acgggtcagg ccgaccgtgg
     3781 aataccgcag ccgtcgtctt tttgacggca aggctatgcc gcgaaagcgg cttgaagggc
     3841 gttcatcaga aaagggcgac gggctggcgt acactgtcac cgacgccagc cgggagcggt
     3901 aaaagcgccc gtcccgtgcc cggccggacg cgggacactg accacaggca gcgtttatgc
     3961 cgcgccgcgt cagccggcgc agtaaaaaga ggagcacggc gacatccttt gtcggaaaag
     4021 aaacgggcgg ttgcccgccc gtctccgtca tacttcatcc gccataatgc gccacattgt
     4081 cgattatcca cgcaggtccg gtgacttccg gcagctcatc atcatctgtc ggggaagacc
     4141 attccagttc cccccacagc acatcaacga gctctccgcg ctcatacaac tgccagccac
     4201 agaaattgca gccctgttcg gcgtaccagt gctccagcgt gcagccgaaa cggcggctta
     4261 attctgcaac aacgtcgctt tccggctgac accacggcgt gtcgaaatct acctgaatga
     4321 agttttcccc ctgactgctg atattcaggt catacccaca aggccacttc acgccatacc
     4381 gttctgtata ccagtgatat gcagaaagaa caccgttcag aacaccgccg ttaaagccat
     4441 tcacttcaat atccaggcgg gtcggcaaaa ccgtcagcag gtcaaacggc atggtttttt
     4501 ccggcagtac gttgtcacac agacggttcc accacaccga tacatcctcg ttaccccaga
     4561 tgtcacacca gtcacctctt tttgcggtga aatgcacagt gatagtttcc ctgacatcat
     4621 ccggtaactc ttcccagcga cgctgtccag taccgctctg cagccagagt tcatgcagca
     4681 tcaggcagtt ttgctcatcc agcagtacac cgttctgcag gtgtgtcagc cagcgggtga
     4741 acgcgatatt ttccggtgat acagcaccac gtccggcagc cgtcactccg gggcactgct
     4801 cagagcggat attttcagtg atctgcagaa gtccggcact tccggccaga aacagctgaa
     4861 taccttcatt tgtggcgcgg cgataaagcg gtgtgactgc accgctggcc agtcgtttaa
     4921 tctcagcgat ctgtgccggt tcaccagaaa aatacatacg attcgagcac cagtttggca
     4981 tgggaattct cctctcacat gcggcctcca tcaggccgtg ccgcacctgt aaccgggcga
     5041 ccgcatacga atgcaggtgc ggccccgaag ccgcagcgcg tggaggagga acgacgaaac
     5101 gtgaccgggt gaggaggccg gcgacagcga cacacttgca tcggatgcag cccggttaac
     5161 gtgccggcac ggcctgggta accaggtatt ttattcacag aaccgtgcgc aaaatgctgt
     5221 ggataagctg gacacagcag cccacagcag gcatacagcc acacactcag agaattgagc
     5281 gttttttagt cacaataatg acatttgact gtgtgtggat tcagttatta gaatactgta
     5341 tatgcataca gcaatagaaa agggagatga gaacatgatc cgcattgaaa ttcttttcga
     5401 ccgccagagc acaaaaaacc tcaaatcagg cacacttcag gcgctacaga atgaaatcga
     5461 acaacgtctg aaaccacact acccggaaat ctggctgcgt atagaccagg gcagcgcccc
     5521 gtctgtatct gtcacaggag cccgcaacga caaggataaa gaacgtatcc tgtctctgct
     5581 ggaagaaatc tggcaggacg acagctggct gcctgcagca tgagtaccgt ttatcaccgt
     5641 ccggctgacc cttcaggcga tgacagttac gtgcgaccgt tgtttgccga tcgttgccag
     5701 gccggttttc cttcgcctgc cactgattat gctgagcagg aactggatct gaacagctat
     5761 tgcatcagca gacctgcagc caccttcttt ctgcgcgcca gcggtgaatc gatgaaccag
     5821 gctggcgtgc agaatggtga tctgctggta gtggacaggg ccgaaaaacc acaacacggg
     5881 gacatcgtta tcgctgagat cgacggtgag ttcaccgtca aacgactgct gttgcgccca
     5941 cgcccggcac tggagccggt ttcagacagc ccggagttcc gcacactgta tccggaaaac
     6001 atctgtattt ttggtgttgt cactcacgtg atacacagga cgcgggagtt acgctgatgt
     6061 ttgcactggc tgatatcaac agtttctacg cctcatgtga aaaagttttc cgcccggacc
     6121 ttcgcaacga accggtcatc gtactcagca ataacgatgg ctgtgtgatc gcgcgcagcc
     6181 cggaggcaaa agctcttggc atcagaatgg ggcagccctg gtttcaggtg agacagatgc
     6241 gcctggagaa gaaaatacat gtattttcca gcaattatgc gctgtaccac agcatgagcc
     6301 aacgggtcat ggctgttctg gagtcgcttt ctcccgcagt tgagccctac tcaattgatg
     6361 aaatgtttat tgatttgcgg gggataaatc attgcatctc tccggagttt tttggtcatc
     6421 agctcaggga acaggtaaag agctggacag gactcaccat gggggtgggc attgcgccta
     6481 caaaaacgct ggcaaaaagt gcacagtggg caacaaagca atggccacag ttttccggtg
     6541 tggtcgcgct gacggcagaa aaccgtaatc ggatcttgaa gctactggga ctgcagccag
     6601 ttggtgaggt ctggggagta ggacgcagac tgacggaaaa gctgaatgcg ctgggtatta
     6661 acacagcact gcagctggcg caggctaaca cggcattcat ccggaaaaac ttcagcgtca
     6721 ttcttgagcg tacggtacgc gaactcaacg gcgagtcctg catatccctg gaagaagcac
     6781 caccggcaaa acagcagatt gtctgtagtc gcagttttgg tgaacgaatc acagacaaag
     6841 atgccatgca ccaggctgtt gttcagtatg cagagcgggc cgcagagaaa ctacgtgggg
     6901 agcgtcagta ttgccggcag gtgacgacat ttgtacggac atcccccttt gcagtaaaag
     6961 aaccctgtta cagcaatgcc gctgtggaaa agcttccatt gcccacacag gacagccggg
     7021 acattattgc cgccgcatgc agagccttaa accatgtctg gcgtgaagga taccgctata
     7081 tgaaggcagg agtcatgctg gctgatttca caccatcggg tatagcgcag ccgggattat
     7141 ttgatgaaat ccagccccgt aaaaacagtg aaaagttaat gaaaacactc gatgaactga
     7201 accagtcggg aaaagggaaa gtgtggtttg cggggcgagg aaccgcccct gaatggcaaa
     7261 tgaaacggga aatgttgagt cagtgttata caactaaatg gcgagatatt cccctggcca
     7321 ggctgggtta gttcagtcat ctccgaacat attttcagcg tttcttctgg tctggtcttc
     7381 accgtcattt ctcgacagac tctgctctgt aagaggagta ctgcaatcca cagacgtatg
     7441 gagaccagat tgtttttcca gtaattcaag caatttcttt tcaaccgaaa aagcacctgg
     7501 tatcacgctg gaaatcacct tattaatatc atccagagtc gtattttctg tcagtaactc
     7561 cgatatcagc aaaggaagac gtttatctat agctgcaaac gccgcaccag taactaacat
     7621 cgcgcgcatg aaacgtcccc gctccttcag tttttcattt gcaatgagct catctgctat
     7681 cttatctcct gtccgttcat ccgggaaaag ttcaaatgag cagcgtctgg atttattttc
     7741 atcactcatt agtcacacca cctttcattt ttatcattcc catgactaaa tcaaactgcg
     7801 gggatgagct cagataaaag cgctcatcag gaaccttagt caggttcttc acagcctcgg
     7861 ccacaatttc agccccacca ccaacacaca ttacatgtgt atagtcggtg aaacggctga
     7921 tgacatcatt aactcgttta ataagcaact tctgccgttc attgataaca ttaatgatag
     7981 actcacgctg ttcagcatta taaatgcgac gggaaaggta gtcaggttca ttacggtgca
     8041 caatgatatt gtcagcctgg aatgagctca ctcttgtatt agcatgtaca gccatctgtt
     8101 ccttaacccc ggatgtaatc agggacacgc cgatatttgg atcacaccag gtttttgtaa
     8161 tccctgtcat tttgctacga acatgcgaca catcaagagt tgtaccaccg agatctacga
     8221 tcaaaaggga ttcatcatcc tcaagccccg caagaacact gaaaccagcg ggaatacttt
     8281 caggaagaac ggaaacactg cgaataacaa atgcatcact cccctgcacc cgtacttcac
     8341 gcatcacatt ttttttcttt ctttcaatgt tttgcttatt tttctgattg ttggcgtcaa
     8401 ggtattcaga gatcggcaat gtgacgatga catcaacagg ctgcgctttc aggccggtct
     8461 gctgtagcgc atgctgaatg gccacaacat tcacatcact gtactgatag cgtgtttcag
     8521 tcgttacgac agcatcagca cttaatggat caaatgaaaa cttctcgccg tcgatctcgt
     8581 aattagccgg ggcagcatca tccagtaaac taaaagacca ttccggttta aagctgttag
     8641 ggctgattaa cgttttcacg tcaccatcct ccaaccaggc aagtttaatg ttggtagaac
     8701 catcatcaat aaagattttc ataaccagtc tccgttgctc ataaacaaat agaaaaaaca
     8761 aacatgcttc atacatgcaa tgtatattca attataatga aaaaacggtc tttctgaaag
     8821 gacaaatgca acataaaaac acgataaatt gcatgtatat tgtacacaca actttaagac
     8881 cagaggaaat catagataca aaaacattat gaaggttata cgtgatacat aaatgcaata
     8941 taaaaacatc gtggacatca tggttcaggt taggggaaaa ggaatgaaat ggtgaaactg
     9001 agaggaaagg aaagcggtga cgaatcatgt caccataaac agaaagatgt ctaatgggga
     9061 ctctttaggt acgtgaagaa tccccataac gcatttaaaa gacacacatc ggataccctt
     9121 aaggtatccg atagtttatt gacctattgc accatcacgt tcaagcttct ctcttatagc
     9181 ctcaataata tatgagctaa aatccagaga tgttttactg gtcgctttaa gttccgcatg
     9241 agcttcaaaa tacgacagcg gtatggcctt aagattctta gccttcgtcc tggcagccac
     9301 gggacgacct gaaacttcct ctgcaccttc atttggctga gacggggctg ttgctgttac
     9361 ggcagaagaa gaagtttgta atctggcaga atccaccagg tttttcagtt tttcagcctg
     9421 tgcactaaat ttcgctttgc tcatgagata acccaggatg tttgttagat acttaaaaga
     9481 tatccattaa aagcccaaag gatactatat ggatacccaa tagacacacc ttactgtttg
     9541 ttattttcaa tcagatgatt gatttcttcg atcagggcaa taacttcctt acctcctgcc
     9601 gaatagcgcc catgaacaga ctcagtgatt cccatcccag tctcaatgac atcctcaaat
     9661 tctgcacgag cagaaatgcg gctcttcatc agcttcagat gtttgaacga tggcaggatt
     9721 gcatcaagct tagggaattt cttcttgtta ggattaactt tacagagata caggtgagca
     9781 gttatatcgg tccccatgat agagctgatt tcgtccagta ctccatcaaa atgcatcaaa
     9841 ccaatacgtt cagtcaggga gtcttttgaa gggacaataa cgcagtctgc aaaggcaatc
     9901 gcagtacggg taagatctga gtcaaatccc ccacagtcaa taagtaacgt cttatcgctg
     9961 ttcttgtagg gagtcataat ttcgattaac tcatccactg ttttgggtac tttcacggaa
    10021 atttcttttc cttcaggtct cagccccaga atgatagaaa gcccggtatg agtatccaga
    10081 tcaactgtat cagacttcat ttcaccaaca atgtttacgg atgaagttgt cttacccaca
    10141 ccacctttgg gatgcgtatt agcaataaca ggcatagtgt atctccagaa tccacatgta
    10201 atacccataa agaccccata agacaccttt cggatatcca agaggtacta aatagatgtt
    10261 tttttactct atgccggtcc tgcatgtcaa cagaatatga aataccgata atatatccat
    10321 gagatatccg aaagggattt aaaaaagtca gagcttatgg aattcttttg agcatagaca
    10381 ccgcatcgga ctcaggcatc gaaaactgca cccggtgccg tgcagccaca tccagtgcaa
    10441 acaccttcgt gtagacctcc gttgagctga tggacttatg ccccatcaga ctctgcagga
    10501 ccttcagcgg tataccggca tacagcatat gcatcgcata ggaatggcgg aacgtatgtg
    10561 gcgtgaccgg gacagagaac gtcacaccat cagcggcagc ggcggcaacc gcctccccaa
    10621 tccaggtcct gaccgttctg tccgtcactt cccagatccg cgctttctct gtcctgcctg
    10681 ttcgtttatt acgccgttcc atggggattt tcagtgtggc caccatcgtc tgcagctggc
    10741 tgacgtacca ggcgtcggag agcggaacca gacggtgagt ctgctgaccg acgggcatcc
    10801 ttcctgccgt cctggcggct ttttctgtcc gctgcttcag agtggccagc tgcacaaacg
    10861 gatacggagg cgcaagcgaa aaatcccccc gcgtcagcgc cagtgcttca ttaatgcgcg
    10921 caccggtgtt ccacagtgtg gccagcagca ttttgcggcg cagatccggc acgtaatgga
    10981 gcagggcgct cacttcaggg gccagcaggt attttggcag ttcatcatga accatcgaca
    11041 tctggcggag tgccagcgct gccggataat cgatggcaac aggaagctga gcagcatccc
    11101 gggcatggtg gatcggtgcc ggcaggtgct gcatatcagt gttcactcca cggggaaacg
    11161 tcgggcaggt tttccacaac acggtacttt cccggataac ggagctggtt acctccacgc
    11221 gtccagacgg tgacaacaaa accccctccc tggttgtttt cggggagcag gcgatcgcac
    11281 tgctcactca gcatgtggat cacaaacggc gcatcgctgc cgtcttcaaa taccagttcc
    11341 agcgcatcgc ggccatgcag tgctccctta ctgatgatga cgtactcagc cccccgcatc
    11401 tccctgagta aatgttttgc cgcatccggg acaagcagcc tggctgcgcc ggcattccag
    11461 ctgaggtaga catatccggc ctgcgcctgt acagactgcc agtaatccgt tccggcgaca
    11521 gcctgacctt cattctcaat aaaaaacatg ggtgactcct tttttctttt ccgggcaggg
    11581 acggtcatgc gataacgtca tccatcatca ctgcccttac cgtcacgccg tctgataaca
    11641 tcatgccgtt atcatcatca ccatgataac aagcatccat ataaccacgc cgttaaatta
    11701 ccggagagcc gtttccgtca gatccgactt tccggaatat cacaactttc cggaagtgta
    11761 ccacgctgag aaaatcatca tatcgctgtt ttccgcccct gcgcggcgca tcacaggtca
    11821 gggagatgac acaacatata gtgtcatcag cagctgaatt cagcactaca tgttgtgctg
    11881 acgggagatg gcgctgtgcg gagggagagg aagatccgta accgccggaa accggatctg
    11941 cacggcggga aacgggcaaa tgatatgata attaagcaat tcacataaaa tcatatcaga
    12001 tatgattttc atcgttaaaa attaaacaat atctgatatt atcagaaatc agaagcacac
    12061 aaatcatgca ggatatgatt tttacctgtc agaacattat cagatggagc gtgcaggatg
    12121 gatgaagaaa gggtgttttc actcagctat gagcagttga cccgaatagc ggaaaaaaat
    12181 atccgtgaat gtgggctgga cagccagagc gccaggtgcc tcaatgaact gagggcctca
    12241 gctctgctgt ggttgtggta tgaactggcc attcatggtg cgccacagaa caatcacgca
    12301 caggcccggg aacgcatcga taccgattat cagcgcctca aaaaactcat ctggtcggag
    12361 ggtgacagtt aatgactcag agccgccgac cgtcgccact gcagcggaga gtgctgattg
    12421 tcctggctgc ccttgatgaa aaacggccgg gacccgtgct gacgcgggac atagaacggg
    12481 tgctggagcg aagcggggaa gccccggtct acgggccaaa cctgcgcgcc tcctgccgtc
    12541 gtctggagga tgccggctgg ctgcgtaccc tgcgcgctcc gaacctgcag ctggcagtgg
    12601 agctgacgga cgccggccgg gccgtggcac agcctctgct gctggcagaa caggaccgcc
    12661 tccgggcaga acagcgtgcg gcagaagtcg tcgtactgcc gctggtcccg gcagcaggtc
    12721 tgcccgcaga cggcacttct gccactgatc tggcggtgga gctgaacggc ataacgtatc
    12781 aggcctgccg tggggatttt gtggtgcgtc tcgacggcag cacctgtctg cagttatgga
    12841 ataaagaagg cagagtggta cgcctggagg gcgatccgct ggaagtggcg cagtggctgc
    12901 aggcctgtca tgacgcagga atagaggttc gcgtacagat aaatgaaagt agcgtcccgt
    12961 aaagggggct tgagtaaagc ggcgcggatg gctgattatt tccaagaata atcgtcagtt
    13021 caggtatcgt ggatacctgt aacagcctcc tgtgtacgag ctaaatccac acatcttctg
    13081 ccgtagccag gaacctactg gctgagtatt tctggtggca gagctacgta agtagccgta
    13141 cctgtatagt aatcagatat ccagagaaaa cgtaatgaaa gatacaatta ctccactaaa
    13201 ttacactaag gaagccataa aagacctgtc tgtcatggaa aaaggattaa ggtcaaatgc
    13261 acctcttaca tcatatatta tgcgtcaggt cattgatatg ctggacagta gtgttaagtt
    13321 catcttaccg aactgctgcg atattattga accagaagaa taccgccaga cacatttcga
    13381 tttggcacga ttaccatatc cagttgtaac atttgaaata ccatggttca aagacaatgt
    13441 agaaacccaa ataggtgatt ttaatatatc accatcatcc agaagaattg ccctgtgctg
    13501 ggaggccaga cagagttttg aacccatccc gggatgtaat tcaatcctta acacttatat
    13561 tgatggcgga gtgtttatcc ttcctgtttc atggtctgat gatttaaaaa tatggattct
    13621 tggtgtcggt ggaatgtttt tcccatataa caacaaactc actaaatacg aacctgacag
    13681 gacattgcct gcatccaggc ttgtaatcga cacattaaaa gagaatggag tagccaaata
    13741 taatgcagca cacttcaaag cagagccttt tataacaagc atggaattta aagatgattt
    13801 gataaagcag gtgggaagta ttgaaagatt atatgcgcaa attattatgg atactcgcga
    13861 tgaactacag gctttcatac aggcatgtag cgtgttaaat tgcgaaaatg tgtgtcctgt
    13921 cacacttagc actaaaccag aaagaaaatt cataaatggt cgtaaagttc agccacctga
    13981 aaaaaacaag cgcccttcat atacttataa agtattgcaa ctgtctgaaa ctaaggtgca
    14041 aagtaatcat actggaacag gaaaatccgg tgggacgaaa aggatgcact tacgcagagg
    14101 acatattcgc aggaaaaata acaaactaat atggatcaga ccagcaatga ttaatgcaaa
    14161 cagtagagca ggcattgttg ataaggatta ccagataaat ataagaaaag aagaaaacaa
    14221 accttagtaa atcttactgt gtaagtaaca atccgttgta tcctgctata aacgttcctg
    14281 attcacatag cagggtacag agtcatcttc gtaaattatc aagggatatt acataacaga
    14341 aagtttatta aattttgaac tatgatatta taaggaagta gaacaacata gccgaggtat
    14401 ttatgggaaa atatcaggaa gaaaggaaaa ctccggtcat tgtggtgaaa aaacgtcgta
    14461 ctttttcgcc accgtcctta tccgaaaaaa cagatattat cgcacctgta tttactgaac
    14521 agacggcaga atcagccccc gccggaataa attcatctgc cgtcgaaaca catatccccg
    14581 aagcgccagc caggaagaag aaaaagaaaa gacaccgttt tccacgtccc tcccactgga
    14641 cccgtgagta tactcacgag tgcgtggaaa aaataaaggc cctgtttcct catcttcgtg
    14701 ctgaaggtgg tggatttatt cccctgaaaa ttggcataaa caatgatatt tcggcatttc
    14761 tggcagaaca cccggagaca gaactgacca tggacgaatg gctctgtgca gtgtcctgca
    14821 tcacatcccg ccgcgtatat ctgcagcgga cagctgtcgc cggcgtccca cgttatggcc
    14881 tggatggtca ccccaaaggg caggtctcag actctgaagc acagagtgcc ggtcgcagac
    14941 tggcgacatt agaacaaaag tggctcagga tgcaggcaca gcaggagaat atttctgggc
    15001 aataaacact cctgataaca ggtggacatt acgtatccaa agattctcct gatgcgaaac
    15061 aggagcttct tgagcgtctt ttgtccggaa aaagaagacc gggcgggatg aaatggatat
    15121 caagcgcatt ctaaaaacaa aggaagcact tgataacatc aggaatttta ttgataaaca
    15181 gcagttaacg aatagtgata ctccgaagaa aagcacggtc ccaaatgaag ataaaaaagg
    15241 cataagatga tgatcacagg aaatggaata aacactgtaa ctgtcaatgg aaaagtaaaa
    15301 cacatcacag aactggatga tataacactt tgtttagagt ggacaaaact acgagaggaa
    15361 aacaatcgtc tttatgaaat taataaccag gcaaacagag gatggcgtgg tttcatccta
    15421 agattgatag gggttaacct gccagataaa aggactgagt ttacacaacg tctcttattg
    15481 acgcgcaaaa tttcgggttc ggtcatgaaa aagtaacata ttctatgctg gaacagacca
    15541 attttgcatt cgccgtccag gagtttgagg ggaaagtagt cagtgtttac gctcagtcag
    15601 ggaaagtgtg caatgataaa caacacgaaa ggaatcgtag tacatgggca ccgcgattca
    15661 atattcaagg aaaacacaat acagcaatgt acttgtactg tttctattat tctggcgatt
    15721 tcaataaagg cacttgctcg cgctctgtca ttttctgaaa ctcttcatgc tgcatttcga
    15781 ttttccttct aagatcataa ctcgcatacc tgttcttttt atgtatatct atatctattt
    15841 ttgtgacgag ttcaaaccac aatgttcgca ctccccaggc agcgttatga aacatttccg
    15901 caacggctat agagtctgta ttaactgctt cggtcatgta gtcgctaata cgttcagcgg
    15961 taattttcgt tatttcattc atcgtaattc attttatctc ctacaagatt atttaatgga
    16021 atgaaaaata tctggcctga taataatgtt ccagaattaa tatctgcagg ctatgttaag
    16081 ttgtccccac acacaatcta cctgttttct cgtgtgtgcc acaggcgcaa tacataaata
    16141 gtctggccat taagttcgta acggatttcg tactcgccag caaaaatccg tctgacctcc
    16201 ctgggttcaa actgaaacaa ctgttctccc atacgtggat gagttaacag aatgaccggt
    16261 gcctgtgtca gggattgcac cgttctggcg gccgcaggtt tactggccag caccagaaaa
    16321 tcatataacc gcgccaaatc agaaagcgcc ttactggtcc acttcagttc catcagcgtg
    16381 gcaccggtaa cggattgtca gtactgaggc tgtccgccca ggcctgtaca gcctgatggt
    16441 cgataacctg tccggatgtc acatcgtcca gggcttccag cgtcaggcga ttacgctcct
    16501 cctcctgggc aagccatgca gaaagcgcct gtttgataat ccagccccgg gagcgttcca
    16561 gtctggcggc catctggtcg actttatcag ccataggtaa tggcacatgc gcggtaatga
    16621 cttttgttgc ctgtgcggta ttgtttttca tttgcatatc cgattagctg ttaatcatat
    16681 ttaatcatag tgattacttt cagacgaaac aaggcaaata atgcgcgtca gcgcatgtaa
    16741 ggcggcactc cgttgttaca tatggcacag aaacaaaacc gccgaagccc ggcgtcagcc
    16801 ggtactgatt gatagatttc accttaccca tccccagccc tgctaagcca tacccgtttt
    16861 cagccatgac agagcttctg tgcgcggtcg gagtggtccc gacgagggtt tacccgaagt
    16921 cggggcgtat ctccgcgtta gcgggccgtc agggccgctt acgagcgtgt actgaggact
    16981 tccagccaga agactgacag cgatgaggat tcttagttac aacattcata attaaaagcg
    17041 atgttgttcc cgcccttcgg gcgggggcgg cctcgctttt cagttatgag gggtaggcac
    17101 ggtgctgcgt ttgtgtggtg tttttattcc tgatgtcttt ttgttgtggt ttcagaacgg
    17161 tgaggttcgg ggcccgcgcg tcagcgtggg aggtgcatat ttgtctgtcc acaggacagg
    17221 cactgaatag gtcttttttt taaatggaag tagttaagta gtttaaagga aatacagtac
    17281 tggggtttaa aagaaacatt gcaccctgta acgcctgcgg ctggcgcttt atagcattaa
    17341 cggttgtaac cttacggaga agtcccttgc agttaaatgt ggataaacag aattccccgt
    17401 ctgtgagacg tgttttgcgt gaaaaacagg gggagcaagg cgagccggaa ggcagatgat
    17461 gaggctgttt tttgtgtgct gtgctgcttt tttgtgcgtc gccgggcttc agacgtgcgg
    17521 aagcctccgg cgcaggccgg attattcaga tgaggtcaaa ctaagggtga ggcagtggcc
    17581 agccgtgtgt agttgttgtt ccgcgacatt atcatgcgtt ccttcacgcg acggtcgatt
    17641 tcgcgcttca cggcctccag gcttccccgg aaacgccctt ccgcgatttc acgtgtcagc
    17701 tgccgtttca ccagggtgac aatgtcctga cgggtcctgc ctgcatcgcg gcgcgcacgg
    17761 gcacgtttca tgccgtggga cttaagctct gcctgataac tgcggaaacg ctcgcgcaca
    17821 aaacgccagg ccctcgctat cagttcatcc atacccagcg catccatccc ctgctttttg
    17881 cgcagcctgt tttcccattc cacgcggctg cggcgtgcgg aagccactgc ctcttctgag
    17941 atatcgagtg agtcaaacag tgccggcgtg aacgtgatgt cggtcgggat gttacagcca
    18001 attgtcgggt cgtattccgt ctggtaggtt atcagcccca gctcagacag gaacttcagg
    18061 gcacgggtgg cacgggtgat ggagagtttc ccggcctcag actccgtcgc cagtccgcac
    18121 tcaatggcca gcgtggtgat ggagcactgc acgcggttgg ccagcgggtc atagtggaaa
    18181 cacagcccct gcagcagcgc atcaatcgcc cggcggcgca gcaccggcgg catacgccgg
    18241 cgcaggccgc gcgaacgggc atacgccaca tgcgcggcaa aatcaaaacg ggaggtgaag
    18301 cccacggcct tttccatcag tttttcgcag aacttcagcg ttccggcatc ttcacggggt
    18361 gtgaacaccg gattcgggtt ctttacctga cggtatgtgc tttcagtaaa tgaggtctgg
    18421 ttcagggccg ccacaagctg tcgcttatgg cgggaacaga ttgccgtgcg ttaatgcact
    18481 gcaaagggtt gaatctctga cacggtttca tatatactta tcccgtattc tgtgaggccc
    18541 ccattatctt tctctgattc cgccaagttt cgaggaaaga tgatgggggt tttcctttat
    18601 ggtcttctga aacttttttc agccttccgg ccccggcaaa ctgccgtcgt ttctgccata
    18661 catcctgaat gccttatggc gcattctgcc gttttctggc tttccggacc gcgaggatcc
    18721 caaaagccac tgcataacga tccgcaatat accccttttc caccgctttg aaaagccgac
    18781 acgatttctc agagttacag ttgccctctt taacccccta aaaagcgtcg tttttttgaa
    18841 ctctgtcaaa aaaatgaaca atttcacaaa cgatctttta tgctgctttt ttcttctttt
    18901 ccagtagaag agatcgccga atttttttaa acgatctaat atcccgcaca caacatcaaa
    18961 tttagaataa tattctgtct tatcaaatat aacgatctat tcaactgctt atcctcgctc
    19021 ttgccacaga tacatcctta tgtaatatat tttccagcca ttaatcctgt ttgcaggagt
    19081 tgtcggtttt cgcccgcctc gaaaccgtcc acaaggctta caagagtgcg taaatcatgt
    19141 gcctgtcaga actgcaggcc aggctgaacg tgggtcagcc gctgtttccg acacatgctt
    19201 tatgtattcg ccgttcacaa caacatcgaa gcccccctgc ggggatcgat tttcgcggag
    19261 cgttccggat tttgaatatc agtgaattac atcaacgcaa ctggtatctg gcacagtaca
    19321 tccctgccgg taaaaaccgc gaacatctct tttcctggct ttccgaacaa cacgtccttc
    19381 cctggacacc cctgatactg aaaaaggtcc gccgcacgga taaagtctgt ggttaccgtc
    19441 gacatataca cgccgtgttt cccggttact ttttcctgaa agcagacccc gaaagccaca
    19501 gcttcactca ccttcgccgt cacagtgcct ttctggactt cgtgaaaatg gccggtgaaa
    19561 tcaaaaccgt cagggaggat atcgttcagt ctttgatgaa agtgtacccg gatcctgcgc
    19621 tcaatccggc agccagggag gagctggatg ctgcctccac cctctggctg acaaaagcgc
    19681 gttaccagta cctcctccgg cttgatgccc agcctctgcc ggagtcgagg atcgccctgc
    19741 tcctgcacct ggtatctgat gacggagcgt tgacatgaaa attgctgtct taatttgacg
    19801 acttttcccg gcctttatgg ccgggatttt tttcccttct gtttccacaa tgcccggcac
    19861 cggtgacacc tgagggtgta cggagagcgg ctgtcgggca ttttttcagg acgcctttat
    19921 gaccattcta tcccaggaaa accctgtcat tctgttactg gagaatatgc tcgaagcact
    19981 ctccaccgcc ccggacaaca taaacaacga acggcgtcgc cggcgttacc tgctgaactg
    20041 gcttgacact gcccgtcaga tgcgcgagtt cagaggaatg gctgaagagt tcactaccct
    20101 gcgaaaactg ctggccacca attcagacat accggtagca aacaccctga aatcactgct
    20161 ggaaagtggc aataacgcag cattatacga cctgtaccgc ttccgctcgg ctcttgccac
    20221 gtgcaggagt ctgggatggc agattggcac gtgcgcatgg aatgaccagt tactgagtga
    20281 gacactttcc cgcgcacaga ccgggaaacg tcatatcctg cagctgaaca gcaccgggga
    20341 caccttttcc accacgggca gaatgatcag accggtggca ttttcattaa tccatccggc
    20401 caccatcaac acgtctgagg ttgaagagat attccgggat gaaggtttta tgctcgcaca
    20461 cgggcgcgaa tgcagtctga acggcagcgg cagaaatatg ctgtcacgca ttcttcatgt
    20521 gggccattca ggtttagcgg aagaggaatg gggcatggac aacagtctgc ttttacactg
    20581 aaataccggg acagccacaa cggcagccac tctccgctga ctttttttcg caaatcgcgg
    20641 tttgctgatg tggatgtccc attttttatt atccgtcctc tccggttgtc ataaagcgca
    20701 ttccggcgtg aggcttctgt tgttatcatc ccctcctgca gacatcccct tgtcttctga
    20761 tgactgacga tatgcttctt ctgacaatta aagaagcgga agcttcacgc cataatgttg
    20821 tttgactgac ctgccacagg aggaaaaatg acactgacgt tacttaccgc gtataacgca
    20881 ccctatctgg cagccctgac ttttgttctg cttactggta tcgctgaact tatcgccctg
    20941 ctctgcggac actccctctc ttcagctatg gatacacctg atttaccgga gggtctgaca
    21001 ggagaggcgc tggactggct gaacatcgga cggatccccc tgctgatcgt cctctgtatg
    21061 ctggcaggtt tttttggtat atccggcatt ctcctgcagg gtcttattat tcatctgctg
    21121 caggctcctg ctccgaacat tctccttgca ccactttgcc tgctgctgac atgtcctctc
    21181 gtacaccgca ccggcagact gattgccccc tggttacccc gtgatgaaac aacggcgctc
    21241 accgaggagg cattcatcgg ctgtatggcc accatcactg gttatcacgc agtggcgggt
    21301 tccccctgtg aaggtcgctt cacagaccgc ttcgggcagg ttcattacct gttgctggag
    21361 ccggaggccg ggaaagaatt cagacgagga gacaaagtga ttattgtctg ccgtctttcc
    21421 ggttcccgct ggctgggcga actgaatccc tggccggatg ttctttaatc ccgacatata
    21481 cagaggttct cactatgttt aatcttccgg gatggttgtt taccgttata gcgctggtcc
    21541 tcaccctgtt cgttatcggg ctgatatttg cccgcctgta ccgccgggca tcggcagagc
    21601 aggcctttgt ccgcacaggt ctgggaggcc aaaaagtggt aatgagtggc ggagcaattg
    21661 ttatgcccat ttttcacgaa acaattcccg tcaacatgaa caccctgaag ctggaagtca
    21721 gtcgtgcagc ggctgaaagt ctcataaccc gcgaccgcat gcgtgttgat gttgccgttg
    21781 ccttttttct tcgggtaaaa ccatcagccg aaggaatatc cactgcagcc cagacgctgg
    21841 gacaacgcac gctcacacct gaagatctgc gtagtctggt cgaggataaa tttgttgatg
    21901 ccctacgggc cacggcggcc agaatgagca tgcaggattt acaggatgcc cgcgagaact
    21961 ttgttcaggg tgtgcagaac accgttgcgg aagacttatc caaaaacggt ctggagcttg
    22021 aaagtgtgtc gctgacaagt ttcaaccaga ctgcacgggt acattttaat cctgacaatg
    22081 cctttgatgc tgaaggcctg acccttctga cccaggaaac agaacgtcgt cgccgcgaac
    22141 gcaacgaagt tgagcaggat gttgaagtgg ccatcaggga aaaaaaccgg gatgccctct
    22201 cccgacgtct ggagattgaa cagcaggaag ccttcatgac tctggagcaa caacaacggg
    22261 taaaaacccg tacggcagag cagagtgcca gtattgctgc tattgaagct gaacgacgtc
    22321 gtgaagcaga atccgcgcga attctggctg aacgtaagat agaggaagcc gaaatcgaac
    22381 gacagcagat agtgcgtacc cgccaggtcg aagccgaacg ggaagtggcc atacgggaaa
    22441 ttgagcaaca gcaggcaacc gaaatcgcca gccaggccag agccattgcc gtggcggcaa
    22501 aatcggaaga gcagtcacag gcagaagccc gggcaagtaa agcactggct gaggccgtac
    22561 aggcccagca ggatgtggaa accacccgcc agactgcgga ggccgaccgt accaaacagg
    22621 ttgccctgat tgctgctgct caggaagctg aaacgaaagc ggttgaactg acggttcgcg
    22681 cacaggctga aaaagaagca gccgaactcc aggcagcagc aattattgaa ctggcggagg
    22741 ccacccgtca gaaaggtctg gcagaagcag aagcacagcg cgcactgaac gatgccatta
    22801 acgtgctttc ggatgagcag accagcctcc gcttccgtct tgccctgctg gatgcattac
    22861 ctgccgtcat agagaagtcc acagaaccaa tgaaatccat tgacggtatt aaaatcattc
    22921 aggtggatgg tctgaatggc ggtgttaccc gcggtaaaac agcaacaacc gacataagtg
    22981 gcggaaatct cgctgaacag gcagtaaccg ccgctctggc ttaccgtaca caggctccgc
    23041 tcatcgactc cctgctgaaa gaggtgggtt tatccggtga aggacttaca gccctgacac
    23101 gtccactggt catgcctgcc ccggaaaagg ttccggatgt cagcgaaccg gtaaccgctc
    23161 cggataactc agaaccacaa caggtttaat ccccgtgccg gaatttttcc ggcatctttt
    23221 tcacaaagaa tgccctcacg ggatattctg gtttaaaaga ggattgaaaa tgtccgcaga
    23281 aaaattcaga gttgtcatat acaccaatac atgtgaaaag aaagtcagtt atattcctgc
    23341 aacagaagat ataaacatcg ctataatgcc atttcagacg gcagacaatg gcttaattat
    23401 tgtgtgtcag aactcccgta taatacgacg caaagagcgt attcccggaa gcagctgtga
    23461 atggaaagat gtgacattag caccaaaatc cagaaaccgc gttcagttat agcctgacag
    23521 cctgcttttc atcccccata aaagggggta aaaattaaat atttcacaag cgcgatattt
    23581 ttcaggaatg cagattattt tctgttatga ccaattgaga aacaggcatg agaacaatca
    23641 caacacgtga acaattatta attaatggga gagtccacga aagaatcgcc acacatatag
    23701 ttacaggagc tcatgggtat gaaacgcttt gcacaaatgg ctataacgtc agatacaaca
    23761 aggcgcagca actggttgaa agctgcgaaa aaatagctga aggtaaacta cctgttacct
    23821 gtcctgtctg tttcaccata tggcaggatg tacacagatt cacccacgtt gactttgaca
    23881 cacaatcggg gaaaagtgat tttattgata cctgccattc ggagatcata accggacatg
    23941 tttcaataac aggcaagaaa taaaataatg ttccagttct gaaaaacaat atcacttgtt
    24001 ttctggaaac tatcatttgc agcaggacgt ttttcttttt atatttccgg aaccggaatg
    24061 ttttagtttt cataatcacc ccgaacatga atgttcatca tcaatatcct gatgaaatta
    24121 tatttatctg aagacctgtt accagccacc tgtcttatac cgtaacccga tacataccgg
    24181 atttattcta tgaaaaagaa atcacttaca ctccttctgc cgtcattact cagcggctgt
    24241 acggcattta atgccacgcc tgttcctgac accacgcctc cggctctggt gttcgtcgac
    24301 ggacaaattc gcgaaagcgc tgccattatc gcccatacgc aaaaacgtct gcttccgcca
    24361 ccggtgaccc gccctgtgac gccgccccgc ccgacaacaa cagcgacgcc cacaaccgca
    24421 gtcagcagcc agagtaccgc actgcctccc cgtccgctcc ttcaggccac acagctgccc
    24481 gcactggtta tgaccggtaa acccggtcag tcatccgtcg tttatttacg tgaggcacat
    24541 cagcgctccg tcagtgactg gatacgccag ttgctaccac ccggctggca gttccggcaa
    24601 cagaacgctg ccataccggc actgaacaaa cgcctccccg tctggtccgt taatgaccag
    24661 tggcaccggg cactgaatgc gctgctggcc ggccagcagc tttacggaca tatggactgg
    24721 aacaggaaaa tactgactgt cacgacctct gcaacaccgc cggtcgacgt cacggcaccg
    24781 cagggttcac agaaagctgc tgatacaccg cgtaatccgt tcaggggcag cgccgcaacg
    24841 ccggcaggcc cgacaccagt gacacctgcc gtcagcgttt cccgggctca gaccaccacc
    24901 gggacatccg gaactccaga gagcagaaca accacgccag ccccggtggc cacgaaaccg
    24961 gtagtgccac cacagcagaa ggtcataccg ccacggcagt ggaaaatgga aaaggggacc
    25021 accctgaaag acggactggc ccgctggaca tcatccgaaa aatgcccgca cggcacatgg
    25081 acactgcgct gggaaacacc tgtcaactac agcatcgatg cccctctggt tttcaccggc
    25141 agtttccgcc aggcgctgga gcagctcttt gcactctata ccccggctga aaaaccgctg
    25201 tatgcccgga ccagcactgc acaatgcctg ctccgtgtga cagaccgccc gcaggaataa
    25261 cctatgggat gggttacggg tttcattatg gtcattatgg ccattggtaa tttctggttc
    25321 cacagtcacc tcagccgtac tgtgcaccat cagcagaccg ctgaaatcac acagcaggct
    25381 gcagacttta ttcgttatat gaatgccatc aacgattacc tgtatcagca ccctgaacga
    25441 cgtgctgccg gtggtcagct cacgtcagca cagctcggtc tgcccgccac gaaaaacgtc
    25501 agccacctca tcagtcagca gcgcgttttt gtctgggcaa aagaaaaacc cggccttatg
    25561 ggtgccctcc tggaacaaag tggtgattcg gccctgcttg ccagagtgga aaacggtcgc
    25621 ctcctggaca cccgtgggcg gcgtatttcc atcacgctcc ctgctgtgat ccccgatcag
    25681 gtcatcatct ggatgaatta ggccggactc tgaataactc acggatttat ctatgaacaa
    25741 aaaacttctt gctattatga ttgcagccac tctgctgcag ggctgtacca gtttccgaca
    25801 ggcagagaac accagaaaag acgccgttgc tgaacaacaa cgggcccggg aaatgatcgc
    25861 tgagctgcga aaccggacac cggttgtaaa ggaatcccca cgtcagtgga tcaatccccg
    25921 tccggtcacg gataaaaacc ccggtaatgc ggtgcccggc tgtccgatta tcattaacac
    25981 ccggcagagc atcacccttc agcaggtggc ccagcggatc tcggaaaact gtcatattcc
    26041 tgtaaggatc acccctgatg tacaggcgta tctgagcgtg accacctccg gcagtacgca
    26101 gcaaatccag ggcacagtac caccgccgga taccggcggt atggttccac tggccgccat
    26161 aggcagttct gacacgacac ttccccgcct gcagggaaat acggcgtcac ttcctgaact
    26221 gaaggcttca ggactgacat cactgctgaa tgcggtcgct tcccgtctcg ggatttcctg
    26281 gcgttatgat ggttcacata tcacgttcta ttatcttgat acccggacct tcccggtgac
    26341 ctatatggac agccaggttg cctataatgc caccgtggtc agcggtacca tgtcatcaaa
    26401 tggctcatca ggtggaacag catcggatgc catttccggt gatgccagta atacccagac
    26461 caccaccgtg gatatgaaat ctgcgctgta tcaggatgtg aaaaatgctg tctcatcaat
    26521 gctgacgccg ggtattggtc gcatgttcat gtcgacgggc tttatcaccg tcacggacac
    26581 cccacaggta ctggaaacgg tccggacgtt cattgaaaaa cgcaatgaag aaatgaagcg
    26641 ccaggtggtg ctgaatgtgg aaattctgag tatccggaaa acccgtaatg agcaggcagg
    26701 tatcgactgg aacgcagtct tcagcgacag aacgctggga ctgagcctcg gcagcacatt
    26761 caccagtgca gccagcgata ccgtcactgg tggtgtttcc attgtgaacg ggaaactcac
    26821 cggttcaaaa gccttcctga aagcactctc ttcccaggga gatgtcagtg tggtaacccg
    26881 gaattccgcc gtgaccaaaa acctgacccc ggtaccgatg cagattgcca atcagcagag
    26941 ctacattgag agtgtgacga cggataccac cgccaatgtc ggctcatcca cctccctgaa
    27001 tgcggccact atcaccaccg gttttaatat gacccttctg ccgttcattc tcccggactc
    27061 acagacactg cagctgctct attccatgag tctctccgac aaaccggtta ttgagaatta
    27121 cgagagtggt ggaagcaaag cccagctgcc gaatgtggat ctgaaaacca tcaaccagac
    27181 agtggacctg aaatccgggc agacagtcat tatcagtggc ttccagcagt cagggcgtcg
    27241 cagtggaaaa cagggtgtcg gcacaccggg attcttcggt cttggcggtg ggatcaacag
    27301 tgaaaacgac gacaccattc tcgtggtgct gatcacgccc aatatcatct gatttgcaga
    27361 aggaggacta tcgtgacaga tatttcaccg acagagcgac aggtgtttcc gctgacggca
    27421 ggacggaatg tatttcttgc cggaatggac tggaaaacac tgcccgcaag ccataaaaac
    27481 ccccggacgt ttgcccgcag tctgggggca gtacgctata tttcctgtga gtatctcagt
    27541 acggaagata ccgatcgtca catcatggtt gcagccgtca gccagaatac actccccaaa
    27601 ggcagccgca ggtatttctc tctcgccatg cttatcctgc ccctgctcga gccaggagga
    27661 tacgctatcg ttgaactgtc acaggcaaac gatacagaac tttatggttt tgtttctgct
    27721 gttgacggca tcctggtcag tgacctggtg ggaacccggg aggaaatcag ggaggcccgg
    27781 gaaatattcc tcaccattaa cagcgcgcct gaacacggct ggacatgtta tgaaccgccc
    27841 tcgtttaacg ggcctgacgg cagaggtccc ctgccactgg aaaccctgac aggtgcaggg
    27901 aaatatccgg cagaagcacg ccttcacccc gtatcccgtg ggccacagct tattccggta
    27961 ttactcatac tcacccttct gggtactgca tggtatggct ggcagtacta tgaacgcctg
    28021 aaggcagaaa aagcagcact tgctgaagcg gcacaaaaag ccgcagagga acggatcaca
    28081 ccaccgtggc tgagtatgcc ggaaaccgga gattttatcc ggggatgcag tgacgtatgg
    28141 tcacgcctgc cggtgtcggt cgccggctgg cgatattcac tcgccgagtg tgctgccacc
    28201 gggaacagcg gcaatctgcg tgcatcatat acaaacatcg ccgggacgac agtgaccgca
    28261 ttcagccagc gggtcaggga aattttcagc atccggccgt ttatggttat gccggacggg
    28321 aatagcggtg gttttgccct gccggtcacc ttcagtatgc ctgaaactcc cgttgccgtt
    28381 gaggcattac cggaaaacac cctcctgcag gaacggctga ccacactcgc ccggtcgatg
    28441 cagctgaaaa tggactggca ggaagtcagc aacagcttca ccgatgaaga cggaaatacc
    28501 atccagcccc cctggaagga gtatgacctg cagatactga cgacacttcc ggcgcaccag
    28561 gtggcagagc atttcagtga accctccgtc cgttttatct ccgtaacaag gcaactggaa
    28621 gagggtcgtt tccgttatca attcacaggt aaatattatg tccagtaaaa caaaagctct
    28681 tctgctttcc gtcctgatcc tgccgacaac ggtcgcggcc agcaccatca gcaacatgca
    28741 acagaataac cggcaactcc aggtggaaca gattcaggaa gaaactgcac tgtatgaagc
    28801 catgctggca agagaaaagg tgcgacatga actcagaacc ctggagcagg gagaaccagc
    28861 tgagcctgtg cattcacagg aaagcagttc agccgtcacg acagataccc ctgcccccat
    28921 taccggtatt ccccggctga cagagatatc cggagttcac ccccagataa ccgccaggat
    28981 acaactgccg ggcggacaaa ctgctgatgt aaaagaaggg caacacattc ccggcaccac
    29041 attccgcgtg gaaaagatca ccactgacag tgttgtcctt gaacaaaatg gtatcagaac
    29101 aacactacaa ccctgactca gtgccgccat ttacagccgg ctgtcggaac agattattcc
    29161 cccggccctt aatcacaaaa aaaatcgttt ccgggtaccg ctgcccgtaa ggagatttta
    29221 tgctggcacc tgcagaccag ccacctgatg acattctgga atacgtggtt gttgatacag
    29281 ataaaacagg acaaattcat gtcagagtgg tggaaggaaa aaaacacctt cgtgccgtcc
    29341 aggattatct gacccgcctg tgctcccgct atccgggtaa agtatccccg tttgagttca
    29401 ccacacttga tgtgatcgcc cgactcaggc aggagacggt catttccggg gtggaaaacg
    29461 gaattaaccc cgtacagcaa aaaatgctcg ggtatatcag ccatgcggcc accctcagcg
    29521 cctcagatct tcacatcacg ccggggcgtg acagtacgga tttcacgtat ctggaggccc
    29581 gtgtccacgg agagctggag ctgctcgata tcgtcagaaa agatgagggg ctggaattac
    29641 tgggggccac ctattccgga atgactgacg tcataaaggg aacacagttt gatcccggtg
    29701 tcccccagga tgcccgtctg gctgaaagat tcctgaaact ggccggactg tttggtgcgc
    29761 gctactccca ctatccgtgt gtgggcggac tctatgccgt actgcgtctg atcaaggatg
    29821 acagtcagca tataccgaca ttcagtatgc tggggtatca ccctgagcag gagcgcgctg
    29881 tccgaaggat gctgcagcga ccggagggca tcgtgatcct ctctggcccc accggttccg
    29941 gaaaatccac cacgctgagg acggcttctg ccgcctatct ggaacagtat ggcttcaacg
    30001 ataccggagg gatcctgctc ccccggcgac gtctgttcac aatagaatca ccaccagagg
    30061 ggcgtatccc cggtgcaatt cagacggccg ttatggatac cgctcagggc tgggtcgatt
    30121 ctgttaagtc cgccctgcgc cttgatcctg acgctatcct gaacggggag atacgtgacc
    30181 atgcctccgc catcacggcg ataaaagccg ccatgaccgg tcacctgatg ctgaccaccc
    30241 ttcatgctaa cgacccgata aatattcttg agcgtctgga aatggagggc gttcaggcaa
    30301 ggatgattgc agatccgcag ttatttatcg gcctgctcag ccagcgtctg gttcaggtga
    30361 tatgtcccca ctgccgcctc ccctggcatg aggtggagag ctcccggacg gatgaagaac
    30421 gacgcctggt ggaaaacttc tgccagcccg atgccgtgta tcttcgtaac cataacggct
    30481 gcccacactg ctggcgtgga gtgaacggcc ggaccgtcat tgcagaagtg atttccccgg
    30541 atgcgaagtt tttccagatt taccgggaaa aaggccggat tgaggcaaaa acctactggc
    30601 accgggagct cggcggtatg acacgaaatc agcacctgct gggcaaaatc aacagtggtc
    30661 aggtcgatcc gctggctgcg cattacatat cccctgtaga cgaggacagc tatacgctgc
    30721 tgcactgata tgaaaattct ggaaagactc aacaatacgg aactggaact gtccggcctt
    30781 aaccgctggc tgggcaaaaa gacgttcagg cggactacgt tttacgagtc ccttgcaggc
    30841 atgatccggg acggcacacc ggttatgcgg gcactggagt tcatctgcga tgtggaaacg
    30901 gattttggta aaaagaaagg gcagtccggc ctgtattttc tggcgacaga ctgtatcgcc
    30961 agcatacgca gcagtggtca gctctctccg gccctgaagg actgggtacc caaagatgaa
    31021 attgccctga tccgtaacgg tgaagaacga ggtgatatcg ctgaagccat gtttcaggtc
    31081 gtcaaaacgg caaaaggacg acaggagatg attagcagtc tggtgagtgt ctgcctctat
    31141 ccgctcatcc tgctgacact ctgtgtggtg aatatgtata acgttcatac ccgggttatt
    31201 ccgatgatca gcgcctttgc accggaagaa cgctggtcag tacagatggt cattctggcc
    31261 aaatcctccg gactggttat cggttatggt ttgtggctgc tggcaggact gctgctgctg
    31321 gcaattctca tcaggatgtc attcagtctg tataccggtc cccgacgacg gtatcttgat
    31381 cgcatcccgc catggtccct gtatcagact ttccatggcg tgaattacct ttttaatatt
    31441 tcatccatgc tgcagataaa tattccttta tctcatgccc ttaccagaat agaaaaacat
    31501 gcagaacata accgatggtt aaaatcacga ataagtgcta tcagaaaaaa tgtcttatcc
    31561 ggtcagtcac tggcactggc catgaaaaac tgtggttatg actttccgtc aaaatcctgt
    31621 gtgaataatc ttcttttaaa ttcagaaagg gaggacagtg ttgccagtat ggcgctttat
    31681 gcagaccgct ggctggaaga agcaaaaaag aatgtcagaa aaagcggcat tattattact
    31741 gctatatcag gtctgctggt atttctcttc atcatcaata tggtcagtgc catttacggc
    31801 atcagtgaca tgctgaagca gtaaacacag catcctttat attcccctcg atccaaattc
    31861 tgttttacca atctcaggag ctaatatgag cgtcataacc tcttcgtatt cagaacataa
    31921 tattcgtcac cttcagaacg atgataaagg catgaccctg ctggaagttc tcggtgttct
    31981 ggtcgtcgct gccattgtta ttggtgccgt gatgggactg atgtccgata ccctgtcctc
    32041 ttcagacaac cagaaggaac tcaaaaacct gcagacaatc gccaccaaaa tgaaagccca
    32101 aaaattccag gggcagtata caggtactga ctacgtcaaa atcctgaccg aaagtggtgg
    32161 cctgcctgcc gatatgatcg ccggtggtaa taaggcgaaa aatgcctggg gtggtgcggt
    32221 aaccatcaaa gtcagttcag ataaatactc ctacgttatt gaatccagca atgtgccgaa
    32281 gaaaaactgt attgatctgg tcaccagcct gcgcagttca tccatgttta caaaaatcaa
    32341 cggcaatgtg acgaataaag ttgacccgtc tacagtatgt aacgcagata aaactactat
    32401 caaactggaa accaacagtt aatcattcat cagcgcccgc ccgggcgctt tatttaaggt
    32461 cactgtatga acaggttatt tatcctgaca gtgatttttt cactgttatg ctcacttcct
    32521 tccgcacagg catccccaca ggacaaacaa tatgatcgtt gttttattac cgccggaaaa
    32581 cgttattcca ttgatccagg attactcaaa attattgccc gcagggaaag cagtctgaat
    32641 ccccgggcca ttaatcataa caggaatgac agtgggaaaa ttatcagtac cgattacggg
    32701 ataatgcaga ttaactccac acatattccc gggctgaaaa aaatgggcat tatccggagt
    32761 gagaaagatc tgttgaataa tgtctgcctg aatattcaga ccggagcatg gatcctcgcc
    32821 cggcattttc agcgatgcgg cgtaaactgg gaatgcctcg gcagttataa cgccggcttc
    32881 agtaaaagta acactcaccg ccggatgaaa tacgcccggc agatttacag cgcttacatg
    32941 cagggacgtt agtccgggag aactgccatg ctccagataa tcccggcact gctggtcgcc
    33001 ataaccgtgg taatacagtg tctggccgtt cagatgagtc gctatcgact ctcccggatg
    33061 aatgcccgcc cccctcacca tgggctggcg ctgatgagcg ggatgcttgc agctgccgcc
    33121 attatcctgc gtctttatca gaatgacagc gctgtcgggg tgctgtttat gacgataatg
    33181 accggatgta tgacactgat gaccctgacg gatgctgcat cctgccgtct tccccggccc
    33241 ttcaccatca tgtatctgat aagcggcagc gcatttcgtc tctggcaggg agactggctg
    33301 acaggcctgt gcagtgcagg gttctggttc ctgctgttgc ttctgttcag gcaatacacc
    33361 tcttaccgca ggaaaacaga aaccataggc ctgggcgatg tttttctgat tgccggtatt
    33421 gccatctggt ccccgccggg agacatcccc tggattgtcg caacggcagc aggcggagcc
    33481 ttactttatc tgtggtgtaa cagacaacga cgcattacca gagaacttcc ctttgggccg
    33541 tttctctgcg cgtcacagta cgcatttact ttttatcccg gtggcctttg gtaataccgg
    33601 agggaacgac tatgcaaaaa gacaacgata aaggttttgc acttcttgaa attctgggag
    33661 gactggttgt catcagcctg ctcatgccgc tgttctggtc ttacattgaa gactacctga
    33721 atgaaatgcg caaccagagc gccgcatttc atgcagatgc ctacaatact gcagccagga
    33781 cctacatcgc cgataacaat gcccgcctgc atagcggaaa gcttcctgcc accttcacgg
    33841 cggatgagtt aatccggaaa ggatatctga agggtcttaa ccgctcgccc tttgggcaga
    33901 gttataccac cggcatccgt cgtaacacga gtaccggtcg tctggaggcc ctgacctgct
    33961 ccaccggagg ggagaatatc aaagacgatg ccctgcgcag tatcgccagc ctgctccccg
    34021 gtctgggcgg atttatagga aaaaatggca cagcaaccgg tgtttttggt ggatggacag
    34081 ataaacccgg cgactatgga ctcagctgca atggcgggca catcgccatc gtaatgatgg
    34141 gtgatgacct gcaggaaagc gatcgcctgt atcgctttca ggtaccggga cgaccggagc
    34201 tgaaccagat gaacacggcc atcaatatgg gagggaacaa cctgaacaat gcaggaaaca
    34261 tcaatggaca aagtgcaaca ctgaaaggtg atgttacaag cgaaaacggc tggttaatca
    34321 ccaaaaatga taaaggctgg aagaacatca cctatggtgg cggttttacg atgacagaca
    34381 gccagtggat acgcgcagtc ggtggtaaag gaattataac cacaggggaa atcaaaggag
    34441 gaaaagtcag tggggggaca gtccggtctg acggtcgcct ttccacaggt gagtatttac
    34501 agctggataa aacagccgta gcaaatacaa agtgttcacc agatggactt gtaggccgcg
    34561 acagcaaagg ggcaatactt tcgtgccaat ccggtgtgtg ggttggcttt gaatcctacc
    34621 ctgtgggaag ccctatcccg tggccatctg ccactcctcc acaaggctat ctgttgatga
    34681 acggccagtc gttttcctgt tcccggtatc ctcagcttgc ccgtgcttat cctagctgta
    34741 agttacccga tctgcgcggt gtttttatcc gtggctggga taacgggaag ggacttgatg
    34801 gtgatcggaa cagacagctc ctgagttatc aggccgatca atcaggcgtc tatcacgaac
    34861 gtggcggatg gctgaaaggg catcatagtg gtatgccata ctgggcacag gggagtacca
    34921 cagaaatgcg tcccaaaaac attgcattca actacattgt taaagccagc tagggactca
    34981 catctgatag gcatacacag agaaaacacc gttaccacag gcgtaatttt gtgttggtct
    35041 ggagatgatt tgatatgacg ctcccactgg cacggcaaat gaaataaatc tccgggcata
    35101 tcagctggtc agcaaactcg atgccaggcg taaacagacc agcaaaattt ccccttattt
    35161 tgttccctac cctgcaactg tcgggtgcag aaacgggctg tttgtcgtga cactgcacga
    35221 tttcgacctt gaaacccgtg cagaaacccg gggactggca atatctcatg ccagcatgct
    35281 cagtcacctc ctgtccgaat ccttttcatt cgcccttaaa acagagttgc aaacactctt
    35341 gaaacacact cgatcataac gatcgaaata actcgcttcc gtttaaaaaa cactctcttt
    35401 agactcaatt tatgaacaaa acacgctgac ccgcatggag gtgaaatgaa aactgacagc
    35461 acaggtattg ccgcccgtat gatgctctcc ctggacaggg agcgaatttg cgaatgcctg
    35521 ctcagtcatc gccagctgca gtccaccccc ctgcaggttc gttatccgca gggagtcagg
    35581 gatgcccttg gtatcatgag cgaacagctt tctctttccg tctctgacct gacgcgcatc
    35641 ctggtcgaag acgcgttaag tgaaatgttt cttcctgcag ataacatcgt ccgccgtctt
    35701 ctcagcagga tggagcacat catgcaggca catgatatca gcgcaacaac gatggctgca
    35761 ttactggccc cctggaatat ccgccctgcc gtcttccggg agccggatag actcactgac
    35821 tatctgaccg gggagattct tgcagcactg gctgactggt tttatctcag tccggaatgg
    35881 ctgaacggca gggtgcatta tccactgtat cgtccgggag actggccagc cacacaggag
    35941 atattctgca gaattatttc agcccgcgag aatatggaca ttattctctg gcatggtttt
    36001 ccgtttgccg gaacgcattc cggagaatat tgcggcgttc tactcaggca gaaaaaagaa
    36061 attaataaca ccattattta tccggtactt tcgttatatc cggtcagaat ggatatagaa
    36121 aaagaaggct ggtttcagat ggcccgaaaa atatctccgg atatccctgt tcgcgccgta
    36181 acactcactc cggcacaggc agaatatctg ataacaggaa aaatattacc cactgcgtta
    36241 ttcagggttc cgttatcccc ctggtaataa tactgagcaa taaaccggaa taatacacca
    36301 actgttttta atggcacagc cgtgccctct gttgttatac ccaaaatacc tcaatacagg
    36361 aaactgaaat gatgatgaaa aagcatatta cccgtactct catcgcgtct gccgttttat
    36421 tttcctttaa ttcgactgca gcaacgtcat attttgaagc ccgcaatgat gcgatgggag
    36481 gaacgggggt tgcctcctct cactatggtg tggccccact ggcaaacccg gcactgctga
    36541 caaaatccgg tgcaaaagat gacttcagtc ttctgcttcc gtccgtgggt gcgcagctgt
    36601 ccgatccggg taatatcgcc gacaacgccg acaaaatcag cgatgactgg aaagccttcg
    36661 accgtgccgt cgacagccac tccggggtac cgcagacagc ggcccgcctg aaagagagac
    36721 tgcaggactt ccggaacacc catgccagcg cacaggccgg ggtatctgct gtggcggccc
    36781 ttcccggtga taccctcgct gctgcactga tgctgaaaac gctcgggact gtcagcgtcg
    36841 acggtaaagt cagcgatgcc gacctgaact atctggaaag cattgcagac agtggcagcc
    36901 aggatgtgga taaaaacaga ctgacctcac aggcttttgc ccgtgccgca ctgatcaccg
    36961 atgtgggcgt tgcgctggcc actgaactgg aaacggccgg acaaaaatgg tcactgggct
    37021 tcacaccaaa attccagcgt gttgacctgt ttaactacaa tacgctgatc aaaaactatg
    37081 acagcagcgc attcaaaggt gaccgttacc acaacaccca aaacggtatt aacgctgata
    37141 tcggtgccag catggacctg gatgacaact ggacgctggg tctggtcgca cagaacctga
    37201 tcccacgcag cattgagacc aaagaggtac atggtgtcac tgaaaccttc aggatccgtc
    37261 cgcaggtaac gaccggcgta tcatggcaca acgacatgtt caccacggca ctggatgtgg
    37321 atctgacccc ggccagcggc ttcacatcag acagcaaacg tcagtttgcc gccgttggtg
    37381 ctgagtttaa tgcctggaaa tgggcacagt tacgtaccgg ttaccgccag aatctggcca
    37441 gcaacaacgg cagtgcattc actgccggga ttgggctctc accgtttgat gtgattcaca
    37501 ttgatgtcgc cggcctcgtg ggcaccgaca acaactacgg tgccattgct cagttacagt
    37561 tcaccttctg attatcacgc cccgggacgc ccggggcttc tgacctgaat ctgctcgttt
    37621 ccgttcttct ggccactcac cgccagtcct gttcattatt ttccggataa aaccatgcaa
    37681 aaaaaacgta tcacccttgc agggggagtt gccatgctct gtctgagcgg ctgtcatctt
    37741 cccccggata ataccgcacc ggccaggaag tacggtgcag cttctgaagt cgctgtcaca
    37801 cgacatgcac agtatcagtt gtgggagcag ggcacgtatt ctgcagccct gccttcccgc
    37861 cagcccggag gcagcctgca ggctcacagc gggcgggttt cctttgactg ggaaggcgat
    37921 gctctggagc tcctgagtga gctggcccgt ctgcgcggac aacagttcag ttacagcggc
    37981 gtgcgtcttc ccctgcccgt ctcactccat gtacgcgata tgaccttcgc tgatgccctt
    38041 cgtctcacag aggcccagac tgcctggcag gcagtcctct cacagtcagc cgggcgttta
    38101 catctttcct atatgccaac ggagaaaaaa tgaaaaaaca ggtctgtctc ctgctgtccg
    38161 ggctcctgct ggcaggctgt gccggacagc accatcgccc ccctgccggt atggcaggca
    38221 atttaccacc gccggatctc cgggcatggc tgacgcccac tgacaaccag gatgacggca
    38281 tttcagacac ccgtcgccag atgctcacgg acgccggacg aacgctggga ttccgtggcg
    38341 gaaaagcaca gcgctcatgg gaactgattc aggcactgaa tgcccgagaa agtacactga
    38401 atgccctgta tgacttccgt ccccttatca gcagggaagg ctggctaccc cctgttattg
    38461 atgaagcgca ggatgttgct cacattcagc ctgaccagat aagaaccgcg tcccgtgtct
    38521 ggaccatcct gcgccccgaa cgttttgtca gtaacccgcc gggctggcgt gactggcttc
    38581 tgcgggggct ttccactacc gccaccccgg gaactgaggg ccgtgttgta ccggaagacc
    38641 gcgcacagcg cagactctgg gaaaacgcac tccgccaggg ctggcaagaa ggccgtgaca
    38701 acgctgacct caccctggaa gccaaccaga aacgcctgac ccgggattac cggggaatga
    38761 tgctttatgc cctgctctgg cgtcagggca tgatcacacg cccggatgtg actgaacagc
    38821 ggcagaccgt caccggtaac ggcaggaaac tcatcaccgg agaccacgtc cgccgtctga
    38881 agacacacgc ggaattcacc ctgcagaaat cacgctggcg tccggtcgtc agcacggaag
    38941 gagcccctga tgaaattacc cgatgagttc ggcacattcc ctttcgcccg tttcacggcc
    39001 gaagagtttc gctatttttt tgcctggtgt gcccgacacc gcgtcagtga tatcgatctg
    39061 acaggcggct caccggtatc tgtcagtcgc tttggacggc gggtacgttc gtccgcacat
    39121 cctctgccgt cctccatcat gatggcgctg agtgatgaat tgtttggacg ggaagtcctt
    39181 ccccgggtgc tgtccggtca tcccgtggac cgtaccattc aggtcagcgg tgatgcctcc
    39241 ggtcgctacg gtctgcaacg ggggcagcgt gttcgcctgc gctgtcatct gattcaggga
    39301 acatcagcaa cagaagaaaa agtaatctcc gtcaccatgc gggtgatccc gtccgatatt
    39361 ccggacatcc tcaccatgaa tattgaaccg gacctgctgg atgccatgct ccggaaaacc
    39421 ggtctgggtt tcatctgcgg agagaccggc tccggtaaat ccacactggc agcggcactg
    39481 taccgctaca ttcagacgca tttccccgac cgcaaaacgg tgacctatga agatccggtg
    39541 gaatacattc tggggggaga gaatgacctt ctgccacccc atcaggccga aatcgggcgt
    39601 gatgtggaaa gttttgcggc aggacttcgc tctgccgttc gccgtaaccc ggacatcatc
    39661 ggtatcggtg aaatcaggga ttatgaaacg gcagatgcag ccgtccaggc aggtaacacc
    39721 ggtcacttct gcatcggcac catgcacacc aaatcacccg gtgaaacctt tgcccggctg
    39781 ctggggttat ttccaccgga aatcagggat tcaatggcag ccgccaccct ttccctggtg
    39841 cagttcattc tggtgcaggt cctggtcaga acaaatgacg gtggcagaca ggccgtccgg
    39901 gaatacatcg tcataactga cgaactgcgt gatacgctct cccggcagtc ccatgcgaca
    39961 tgggggcatt acattgatga aatcattcgc aaggaaaaac ggcggatacg ggatcaggtt
    40021 ctgaccatgt atcagacggg cagcattgag gcatcagaag ctgcgctctt catcccggcc
    40081 ggagaattcc ccaaatgaaa cgcgaaggct tctggcggga tgtcactccg ccactgaata
    40141 tttatggtgt cccgttgccg tttaccatga tttacctgct gctgtttccg tatccgtcaa
    40201 aaacaacatt ctgggtgtgc agcggcattc tgctgttttt tgtgctcctg gggtactcag
    40261 gatggactgt ccggacgctc tgccggcgtc tgtacagcct ggtgcagggt aaacaggcgt
    40321 caggccgtcc ctggtggtac cggcatcgca cagaattacc ccgtgaatgg cgcggaacgg
    40381 aataaccctc ttttgttcat catcccggcg tatcacaacg ccctcatctt taatccccgg
    40441 agaacgacat gaagtccaga tatcgaacct ggcttgctgt cccgcctgag gagacagaag
    40501 ccgtaaaaaa tgcagttccc ccgttaaatg gccgcaaagc tgtcgcatgg gatccagaaa
    40561 aaaagctctg gtatgcccgg gcgggtaccg aactttcact gctggagcgc tggttacccc
    40621 ggccacagga actgtcgatg gatgccggcg atccggtcac tgaatttgct caggtcctgg
    40681 aaaacgccgg gctggttatt cagggattgc cacagatgga cggagccata cacagagtgg
    40741 ccacccggga tgacaaaaaa ggggccaaaa gtggcgcata cagggcctat cttgatggtc
    40801 gtcccgccgg ctggtaccgc gactaccgga gcgccgatga cagtcccaca aactgggtat
    40861 tttccggcgg agaacaacac gatcctctgg ccagactgca tctgcgggca ttcgctcagc
    40921 aacagcgcga tgataacgct cgtaaactgc agcaacagta taacaaacag gccggctatg
    40981 cccgctccta tattaacggg ttgccacagg caacagcaca tgaatacctg acgcgcaagg
    41041 gcatccgggc cgcgcctggc gtgcggctga acaataaaaa tgagctggtt attcccttca
    41101 gcaacggccg gggagaaata cgttcgtacc agcgtattcc ggtcaccggc gggaaggatg
    41161 cccgcattct gaaagactcc gagaaaaccg gcaactggtt caccttcgga accccggaaa
    41221 atggccgccc cctgttattt gcggaaggtt acgctactgc cgcctccctg cacgaagcaa
    41281 ccggccttcc ggttctgatg acagttgatg ccagcaatat gattgccgtg gcagaaaatg
    41341 cccgccaaat ctggactgac agcccctttg tcttctgtgc cgacaacgat catcaacggg
    41401 aaattaacaa aggggttttt agtgcaacaa aggccgcaga agtcacgaat ggcgaagtca
    41461 ttattccggc ctttacggag gcggaaaaag cacagggact gaccgatttc aatgacctgg
    41521 acgccagccg gggacgggac aactttcaga atgccatgaa tgcacagtta aaacatatcg
    41581 gtatccccac acccaacagt gacactgccg accatcgtga ggctgtcgtg atcgacaacc
    41641 tgatattcac accggtaaaa gatgagaagc cacaaatgtc ccctgaaaac agacagagca
    41701 cagccccgga aacggagctg gcgacacagg atacgcctta tgacacccac tccgacgata
    41761 cagaaaacca ggcatatgaa gatgactatg ccgcctatgc atccctgcaa tcagtaactg
    41821 cggagacgtt atccccggat aaaccactgg agaatattgc gtctgccgct gataccggca
    41881 cagagaatac ctctgtgcct accgctggcg accaggaaga cctcaataca gacgccacag
    41941 gtacagacac agctcacaac agcgacacag cagaaacagg aacgcttcct gaacctgccg
    42001 ggatgcagtc cgtgcagcag gtaaccactg aaccgaccgc agaagataaa ccacacgaga
    42061 acgttgtttc cgctgccgat accagagaag aaaatgcctc tgtggctacc gctgataccg
    42121 gtgaagaaaa tgcctctgcc accaccgctg gcaaccagag cacgcaggca gaagaacatc
    42181 cccggagtga agacacaccg gaaacctccc tctccgacgc ggccgggaca cagcacacca
    42241 ccacaacgca gtctgaagaa caagtgcagg tttccatggc cagcgctgac gactggagcg
    42301 catttgcgga agatctcaac gctgacactc cgggggcccc cactgttcac aacaccgaca
    42361 acgcggagac aaacacagtt tctgaaccag ccgggatgcg gccagttcag caggtgacca
    42421 ctgaactgac ggcagaagat aaagcacagg aaaagagcgt tgcgtctgcc gctgagaccg
    42481 gcgcagagca ctcctctgac atcaccgatg acaacaggaa cgcacccgct gaggagctca
    42541 atgccgatgc cacggttgct gttcacaaca ccggcacagc agaaacagcc tcaccttctg
    42601 aacctgcaga aactcagcca gtccggcagg ccaccgctga accgacggct gaagataaac
    42661 tgcaggaaga gcgtcttgtg tccgccactg agaccagcac agcgaatgaa gcagcccgcc
    42721 ctgaaacgcc atcgcttttc agccgtctgc gccgttttct gaccggtgtg aaatcacgca
    42781 cctcagaacc ttccgttatt caggagaaaa agcccacgga ggctgaaaca gcaacagagg
    42841 aggtcgcacc accatcacca ccggaaacca gcccggaaga cacatcgtca gcggaaccgg
    42901 acacgattat ctttgcaccc cgccgtcccg acagtccgca gcggcacaac ctggacgaga
    42961 tccttaaaaa tcttacacac aaagagtttc ccgacaggac tgcgctctac agcgttgacg
    43021 gtgaacccgc cttcctggac agactctact gtctcgaaat ggtggatggc gccagcgcgg
    43081 atgacaaaaa ggtcctggct gcccttgctg tggccaccaa cttctatggc ggcgttatcg
    43141 agctgaccgg cagtgatgcc tttaagcaaa aagccatgca gctgattgtc gaatacgaca
    43201 tcaaggttcg catgaaactc ccggcccagc gggcagcact ggaaaaactc cgcaaggaaa
    43261 aagagatgga gcatgatgcc attgtcaccc acagacccac gccggaactc aaccgccagt
    43321 caccggaaga gacaacaatg gcccaaccgg ctccccctgt gtcaccgaag gcagaggctg
    43381 aacaaacagc aggcagtacg gctgaaacac cgcatccgga agcgccggtc acggcaaaag
    43441 ctgaagcccc ccgggccgac agaccggaac accccgccgc cccgtcgtct gcgccagaaa
    43501 aagcccctgt cagtgcacag ccgcagcaac ccgacacacc ggtggaagag ccgcccggca
    43561 agctcagccc cggtgaaagt gtgactgccg tcctgacaaa ctacggagaa aaagagtatg
    43621 cgcccggaaa agggcgctgc ttttttgtgg agctcaccaa ccgcagtggc aaacgggaat
    43681 actggggaaa cggcctggaa gaactggtca aaaaccatca gaagggtgac cccgtcacac
    43741 tgacactgca ggcgagagaa acattccggc tgtccggtga aacgcgggat cgtatccgta
    43801 atatctgggc cataaccccg gtcagtaacg gcattaccgt taataatgac caacctcatg
    43861 cgggtcagca taccctggag tttccgatgg caacattcag ccaggtgatg ttacagatgc
    43921 aacaacactg gccacagata atgcaggaag ttcgctcccc caaaaatcct cccaataatc
    43981 tgtacattgg tgaagacagg caacctgcat cagcacctca ggatcaatca atgcgactcc
    44041 ccctcgctgg taatgccccc aatgaactga taccagccat cgcctctgcc gataaggaca
    44101 atcgtcagtt gaatcttctg ctggtccaca gcgcagatga tcatctgcag ggcgtggtcc
    44161 gcctgaacgg caccctttac ccggcactgg ccaccccgtc agctgacaac cgtcagctgg
    44221 ttatcaatgc cctgacggat aacggtctgc agtttgccgg ttacggtgaa gctgttaacc
    44281 atgatgagaa taatcatcag cgtccgtccc cacaaataat gcagtttcac ctgaagcaac
    44341 aggactcccc gttgtttgcc gccatccaca aaccggaaga gcaaccggac aagcttttcc
    44401 gctctctggg ttttgagcag acctggaaag agtggtctga cagccaaaag gcggaggaca
    44461 ggcaggaaaa aactctgcaa caggcacaat cccacagccc cggccgatga gccggtaagg
    44521 acgaccatga acatgaaaaa a
//
LOCUS       DAJTUN010000017        44327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTUN010000017 DAJTUN010000000
VERSION     DAJTUN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.017"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..196
                     /locus_tag="OO470_000917"
                     /pseudo
     CDS             1..196
                     /locus_tag="OO470_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            395..2083
                     /locus_tag="OO470_000918"
     CDS             395..2083
                     /locus_tag="OO470_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6250253.1"
                     /translation="MQSPLPYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNL
                     SDSKFISSFKSWFESGKIKNIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGE
                     FITYYENGAIESKRHFNNDKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGK
                     LMQENFYQQSVLIGNDTSYYQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKD
                     GKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEW
                     YENGIPESLYPYKNGKTDGIAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKE
                     TLYVNGIRQGVEKEFNDRTGKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLS
                     SLDNPTQIKDNATTGDSSAQFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATA
                     YKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAA
                     QGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2136..3866
                     /locus_tag="OO470_000919"
     CDS             2136..3866
                     /locus_tag="OO470_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250254.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(3926..4417)
                     /gene="yajQ"
                     /locus_tag="OO470_000920"
     CDS             complement(3926..4417)
                     /gene="yajQ"
                     /locus_tag="OO470_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6250255.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4585..5496
                     /gene="panE"
                     /locus_tag="OO470_000921"
     CDS             4585..5496
                     /gene="panE"
                     /locus_tag="OO470_000921"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6250256.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5459..6049
                     /gene="yajL"
                     /locus_tag="OO470_000922"
     CDS             5459..6049
                     /gene="yajL"
                     /locus_tag="OO470_000922"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6250257.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6103..7551)
                     /gene="thiI"
                     /locus_tag="OO470_000923"
     CDS             complement(6103..7551)
                     /gene="thiI"
                     /locus_tag="OO470_000923"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6250258.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7757..7999
                     /gene="xseB"
                     /locus_tag="OO470_000924"
     CDS             7757..7999
                     /gene="xseB"
                     /locus_tag="OO470_000924"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6250259.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            7999..8898
                     /gene="ispA"
                     /locus_tag="OO470_000925"
     CDS             7999..8898
                     /gene="ispA"
                     /locus_tag="OO470_000925"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6250260.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            8923..10785
                     /gene="dxs"
                     /locus_tag="OO470_000926"
     CDS             8923..10785
                     /gene="dxs"
                     /locus_tag="OO470_000926"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6250261.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10840..11757
                     /gene="yajO"
                     /locus_tag="OO470_000927"
     CDS             10840..11757
                     /gene="yajO"
                     /locus_tag="OO470_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6250262.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11868..12386)
                     /gene="pgpA"
                     /locus_tag="OO470_000928"
     CDS             complement(11868..12386)
                     /gene="pgpA"
                     /locus_tag="OO470_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6250263.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12364..13341)
                     /gene="thiL"
                     /locus_tag="OO470_000929"
     CDS             complement(12364..13341)
                     /gene="thiL"
                     /locus_tag="OO470_000929"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6250264.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13419..13838)
                     /gene="nusB"
                     /locus_tag="OO470_000930"
     CDS             complement(13419..13838)
                     /gene="nusB"
                     /locus_tag="OO470_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6250265.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13858..14328)
                     /gene="ribE"
                     /locus_tag="OO470_000931"
     CDS             complement(13858..14328)
                     /gene="ribE"
                     /locus_tag="OO470_000931"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6250266.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14417..15520)
                     /gene="ribD"
                     /locus_tag="OO470_000932"
     CDS             complement(14417..15520)
                     /gene="ribD"
                     /locus_tag="OO470_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6250267.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15524..15973)
                     /gene="nrdR"
                     /locus_tag="OO470_000933"
     CDS             complement(15524..15973)
                     /gene="nrdR"
                     /locus_tag="OO470_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6250268.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16124..16663
                     /locus_tag="OO470_000934"
     CDS             16124..16663
                     /locus_tag="OO470_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6250269.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16961..17845
                     /gene="tsx"
                     /locus_tag="OO470_000935"
     CDS             16961..17845
                     /gene="tsx"
                     /locus_tag="OO470_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6250270.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18022..18369)
                     /locus_tag="OO470_000936"
     CDS             complement(18022..18369)
                     /locus_tag="OO470_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6250271.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18498..19469)
                     /gene="secF"
                     /locus_tag="OO470_000937"
     CDS             complement(18498..19469)
                     /gene="secF"
                     /locus_tag="OO470_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6250272.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19480..21327)
                     /gene="secD"
                     /locus_tag="OO470_000938"
     CDS             complement(19480..21327)
                     /gene="secD"
                     /locus_tag="OO470_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6250273.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21355..21687)
                     /gene="yajC"
                     /locus_tag="OO470_000939"
     CDS             complement(21355..21687)
                     /gene="yajC"
                     /locus_tag="OO470_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6250274.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21710..22837)
                     /gene="tgt"
                     /locus_tag="OO470_000940"
     CDS             complement(21710..22837)
                     /gene="tgt"
                     /locus_tag="OO470_000940"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6250275.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22892..23962)
                     /gene="queA"
                     /locus_tag="OO470_000941"
     CDS             complement(22892..23962)
                     /gene="queA"
                     /locus_tag="OO470_000941"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6250276.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24055..24628
                     /gene="acpH"
                     /locus_tag="OO470_000942"
                     /pseudo
     CDS             24055..24628
                     /gene="acpH"
                     /locus_tag="OO470_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24633..26450)
                     /gene="malZ"
                     /locus_tag="OO470_000943"
     CDS             complement(24633..26450)
                     /gene="malZ"
                     /locus_tag="OO470_000943"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6250277.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26606..27979)
                     /gene="proY"
                     /locus_tag="OO470_000944"
     CDS             complement(26606..27979)
                     /gene="proY"
                     /locus_tag="OO470_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6250278.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28055..29374)
                     /gene="brnQ"
                     /locus_tag="OO470_000945"
     CDS             complement(28055..29374)
                     /gene="brnQ"
                     /locus_tag="OO470_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6250279.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29781..31076)
                     /gene="phoR"
                     /locus_tag="OO470_000946"
     CDS             complement(29781..31076)
                     /gene="phoR"
                     /locus_tag="OO470_000946"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6250280.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31134..31823)
                     /gene="phoB"
                     /locus_tag="OO470_000947"
                     /pseudo
     CDS             complement(31134..31823)
                     /gene="phoB"
                     /locus_tag="OO470_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32013..33215
                     /gene="sbcD"
                     /locus_tag="OO470_000948"
     CDS             32013..33215
                     /gene="sbcD"
                     /locus_tag="OO470_000948"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6250281.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33212..36355
                     /gene="sbcC"
                     /locus_tag="OO470_000949"
     CDS             33212..36355
                     /gene="sbcC"
                     /locus_tag="OO470_000949"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6250282.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36481..37662
                     /gene="araJ"
                     /locus_tag="OO470_000950"
     CDS             36481..37662
                     /gene="araJ"
                     /locus_tag="OO470_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6250283.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37805..38713)
                     /gene="mak"
                     /locus_tag="OO470_000951"
     CDS             complement(37805..38713)
                     /gene="mak"
                     /locus_tag="OO470_000951"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6250284.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38838..39749
                     /gene="rdgC"
                     /locus_tag="OO470_000952"
     CDS             38838..39749
                     /gene="rdgC"
                     /locus_tag="OO470_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6250285.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39827..40282)
                     /locus_tag="OO470_000953"
     CDS             complement(39827..40282)
                     /locus_tag="OO470_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250286.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40279..40614)
                     /locus_tag="OO470_000954"
                     /pseudo
     CDS             complement(40279..40614)
                     /locus_tag="OO470_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40769..41053)
                     /gene="ppnP"
                     /locus_tag="OO470_000955"
     CDS             complement(40769..41053)
                     /gene="ppnP"
                     /locus_tag="OO470_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6250287.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41125..41802)
                     /locus_tag="OO470_000956"
     CDS             complement(41125..41802)
                     /locus_tag="OO470_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6250288.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42063..42251)
                     /gene="yaiA"
                     /locus_tag="OO470_000957"
     CDS             complement(42063..42251)
                     /gene="yaiA"
                     /locus_tag="OO470_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6250289.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42301..42825)
                     /gene="aroL"
                     /locus_tag="OO470_000958"
     CDS             complement(42301..42825)
                     /gene="aroL"
                     /locus_tag="OO470_000958"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6250290.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43008..43466)
                     /locus_tag="OO470_000959"
     CDS             complement(43008..43466)
                     /locus_tag="OO470_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6250291.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43586..>44327
                     /gene="proC"
                     /locus_tag="OO470_000960"
     CDS             43586..>44327
                     /gene="proC"
                     /locus_tag="OO470_000960"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6250292.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKG"
BASE COUNT        11510 a        11540 c        10866 g        10411 t
ORIGIN      
        1 tcccgcaagc actataactt tcattgcaca aatcgatcat gttgagctaa ataaaacagc
       61 attatctcat gatatgaaac tcgttcttgc cgatcgtttt tatttcaaaa ccagtaatcc
      121 ttgcgaaaca aattcaattt ctgctcgacc agaatcaatg gatattactg acaaagccta
      181 tgccgacaaa ttaaaaaaat tagaaactcc ttttcttgat gatcgctatt tctttatgac
      241 catcaatcag tgtgacggta atggcactcc catgctgaca gacataaaag tatgtacaac
      301 agaattatgt ggtgctgaat atacaaagaa aatgtctact gtatggctcg atgaaacttt
      361 aaaaccaact gctaaacgtc tggcaattta ttttattcaa tctcctttac cctatgacga
      421 ggagaaaaaa caatggaaag tcaccggatg gtatatctca tcaactgata atcagtcttc
      481 aactaaacag attgcatttg aaggatatac agacacagat aacctctccg attctaaatt
      541 tatttcttca tttaaatcct ggtttgaaag tggaaagata aaaaacattg ctaactataa
      601 tccaaaagga gaaagagatg gaaaatacga aagttattac aatagcaatg gtaacgtcta
      661 tgaatctcta agtttttcta atggtcttat caacggtgag tttattacat attatgaaaa
      721 tggggccatt gaaagtaaac gtcattttaa taacgacaaa attaaagatg gtgaatgccc
      781 tcatttttat gaagatggga aaatcaaaca gcaacatagc tatctgaata gtaaactcga
      841 tggtcctgca tatgaatatt atcctgatgg caaactcatg caggaaaatt tctatcagca
      901 aagtgtgttg attggaaacg ataccagtta ctaccaatcc ggaaaaataa gctctataca
      961 caatagaaat tcccgtggtc agtatgatgg agctaacgaa cgttatagtg aagagggtaa
     1021 gttactgtct aaatccgtct ataaagacgg aaaacaaatt tctgttcaaa cctggtatga
     1081 aaatgggcag aaagaagagg agaaacattt tgatgagcaa ggccaattaa atggtctggt
     1141 taagcaatgg tataaaaatg gtaatcttgc aaaatcacaa aactataaac atgacatact
     1201 cgatggtaac agtgaagagt ggtacgaaaa tggcatccct gaaagtttgt acccttataa
     1261 gaatggaaaa accgacggca ttgccaaatc atggaataag tatggtaaat taacctattc
     1321 aatcgaatac aaaaatggcg tagagaatgg ggcatatcgt aactggagca aaaacacagg
     1381 taaactaacc aaagaaaccc tgtacgtcaa tggtatcaga cagggagtgg aaaaagagtt
     1441 taacgatcgc acaggtaaac tattaacctc cacgcaatat gttaataata aaagacatgg
     1501 tacggaggaa acttatgatc aaaacggaat aaaatatatt acatgctatc aaaatgatca
     1561 gaaattatcg tctttagaca atccaacaca aataaaagat aatgcaacaa caggcgattc
     1621 cagtgctcaa tttgcattag ggaaatacga attcatctgt gcaaatatag atgaagggat
     1681 taaatggtta accaaatctg cagagcaaaa aaacactgat gcgatatact ttttagctac
     1741 agcctacaaa ggcaatggta tacctgcaaa caatgaaaaa tacctagcat atctgcaaca
     1801 agctgcaaca ctgggcagta gtaatgccca ggcggaaatt ggctatttat atctcatcgg
     1861 aaaagaacta ccacagaatc ttcctgatgc tggtgtctgg ttcaaaaaag cagctgcaca
     1921 aggtaatttt gccgcacatt tctatctggg aaggatgtat cagaatggtg acggcgttga
     1981 gagaaatatg gaaaaagcca ggtttcatct atctaatgcg gcagaaggtg gaatagaacc
     2041 tgcgcttaaa gcattaaatg aacttgaaca tcaaacaaaa taaagcgctc agggaccacg
     2101 taaggttcat caaacagaaa aggatatagt ttgcgatgaa agcgaaatct actttgttat
     2161 taatgatttt atctgttttt tattctcatc aggttttatc cagcgaaccc actcagccgg
     2221 atgtttattc tgtcgtcgag aaaaaactaa actctgcttt acctctggaa gaaaatacca
     2281 ccttcagatc gcaggctgaa tggtttaatt tgcaatatga attattagta tctggctacc
     2341 ctgaacgtgc ttacaattta ctgagcaaac ttgagacaga atcgaaaaac gctaaatatt
     2401 atctggattt aactgcctcc agagtcgctg aagaagaagc accagagaca gccttaactt
     2461 ttttatcaaa aattgggtta aataaaccgg ctactcttac atcctttgag agctatatca
     2521 atagctggat aaaaagcaaa caacccgaaa aggctatgca gcttctcagc ctcgatgaga
     2581 atgccctgaa ttattattta ccgactgtgt tagtcgcata tcagatcgtt cctgataagg
     2641 caataagtat ttataattct gtatatggag ataatgttgt tataccctat cagcaagtca
     2701 ccatgttact tacagttgcg gagggttatc aaacaaaagg cgatataaaa aacgctattc
     2761 attatgccga taaggcgctg aatatgtttg ataaagctat tgctgtagat agcagtcgtg
     2821 aatcccatta tagtgatgag tatttgaaac tcatgaatat ctacgccgcc aatggcaaca
     2881 aagaaaaagc ggtggtttta tctcaacgtc tacacaaagc cattaccgaa tctcaatcga
     2941 attacttaag tactctggaa ggaattttat tgttttatag aaataatgat atgcagcaag
     3001 actatcaaaa cagcctttca aattacatag tatttatcga taaaattttt gccttttccc
     3061 catcaacaag aagtgaatta agcctcatta atttattaaa ttcattaaat gaggtggaat
     3121 tgatgagaaa acgtctggcg tttctgatga cagcaccaga atacgcttgt tatgatagtc
     3181 agtattgtta tgaagacaaa gtcagcgcat taaaaatcct gtatcgtcat catgacaatg
     3241 agctaatgga caaatatttc acgactcttc ttaaagagat cagtaatcaa ccattagaag
     3301 actgggatat aataataagt aatactggtg aaaaatttag tgaagtgggt ctaaccaaat
     3361 atgctcaaca tctcgcatct ttcgctgaag aattttatat aaaagagcaa aaatcacatt
     3421 ctgaaagtga aatgagagga ctattttcat cacttgccga attatatagt tttggtaatg
     3481 atactgccag cgcaaaccga gtgttccatc agcatgtccc tgccttcaat acagatcata
     3541 tgattcaata ttttataaat gccaaagaat ggaacaatgc cagggaatta ttgataaaag
     3601 aggaaatgtt gggtatgcat aatctgatat tgttggagaa tatttgtatg caaaaaaatg
     3661 cagaatgcat ggcacacatt acctttacgc ttaataaatt aacaacacaa ccagcaataa
     3721 caaaaataga tagcgtgggt aatgagcaac tttatcagat tggcaaaatc taccataagt
     3781 tggaaattaa acctgagcca gagcaacagg ttttaattca gtcgctttat gaccgggcat
     3841 caggaagcgc ctctgcaact caataaatgc atggtcaaaa gggccaccac ggtggccctg
     3901 acaatcagta gctatatcca gaaagttaat cgcggaagtt tttgaactgg aacggctgac
     3961 cgagatcgcc accgcgcacc atcgccatga cagcctgcaa atcatcacga gatttgccag
     4021 tcacgcggat ctcatcgccc tgaatttgcg cctgcacttt cagtttgctg tctttgatca
     4081 tcttgacgat ttttttctga gtggcgctct caatgccctg tttcagtttc gcttccacaa
     4141 accaggtttt accgctatga acgatatttt ccggtacatc cagtgaactg ccttcaatgc
     4201 cgcgcttcag cagcttggca cgcagaatat ccagcaactg attgacctgg aagtcggact
     4261 cgctcaacac tttgatggtt ttgctggcgt cgttcagctc aaatgaggct tcaacgttac
     4321 ggaagtcaaa acgggactcc acttcgcgac tcgcgttatc gaccgcgtta cgtgcttcct
     4381 gaagatcaac ttcagagaca atatcgaaag atggcatctt ttcctctccc ttcatttttg
     4441 atgcgaagca taatacctgc aaagttaaat aaccaacagt cctgcaccag gacgtatccc
     4501 ttaattgtgc atagtcgcaa cagcatgcac aattaaggga tagccttatt gaacacccgg
     4561 agtggttgcg ggtgaggagg aacaatgaaa attaccgtat tgggatgcgg tgccttaggg
     4621 caattatggc ttacagcact ttgtaaacag ggtcatgaag ttcagggctg gctgcgtgta
     4681 ccgcaacctt attgtagtgt gaatctggtt gagacagatg gttcgatatt taacgaatcg
     4741 ctgaccgcca acgatcccga ttttctcgcc accagcgatc tgctcctggt gacgctgaaa
     4801 gcatggcagg tttccgatgc cgtcaaaagc ctcgcatcca cactgcctgt aactacgcca
     4861 atactgttaa ttcacaacgg catgggcacc atcgaagaat tgcaaaacat tcagcagcca
     4921 ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg gcaatgtcat tattcatgtg
     4981 gcaaacggaa tcacgcatat cggtccggca cggcaacagg atggcgatta cagttatctg
     5041 gcggatattt tgcaaaccgt gttgcctgac gttgcctggc ataacaatat tcgcgccgag
     5101 ctgtggcgca agctggccgt caactgcgtg attaatccac tgactgccat ctggaattgc
     5161 ccgaacggtg aattacgtca tcatccgcaa gaaattatgc aaatatgcga agaagtcgcg
     5221 gcagtgatcg aacgcgaagg gcatcatact tcagcggaag atttgcgtga ttacgtgatg
     5281 caggtgattg atgccacagc agaaaatatc tcgtcgatgt tgcaggatat ccgcgcgctg
     5341 cgccacaccg aaatcgacta tatcaatggt tttctcttac gccgcgcccg cgcgcatggg
     5401 attgccgtac cggaaaacac ccgcctgttt gaaatggtaa aaagaaagga gagtgaatat
     5461 gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa gagactgaag ccgtcaccac
     5521 gatcaacctc ttggttcgcg gcggtatcaa agtcaccact gccagcgtcg acagcgatgg
     5581 caacctggca attacctgct cgcgcggcgt gaagctgctg gcggatgcgc cgctggtcga
     5641 agtggctgat ggcgaatatg acgtgatcgt gctgcctggt ggcattaaag gcacggagtg
     5701 ttttcgcgat agcactctgc tggttgaaac cgttaaacag ttccaccgtt ccgggcgtat
     5761 cgtcgcggct atttgcgccg cgccagccac cgtgctggtg ccgcacgata tctttccgat
     5821 tggcaatatg accggcttcc cgacgctgaa agacaaaatt cccgctgaac aatggcagga
     5881 caagcgcgtc gtctgggatg cacgggtaaa attgctgacc agccaggggc cgggtacagc
     5941 tatcgacttt ggtctgaaaa ttatcgacct gctggttggg cgcgagaaag cccatgaagt
     6001 ggcatcacaa ctggtgatgg cggcagggat ttataattat tacgagtagt gtcggatgcg
     6061 gcaaacgtcg catctgacca gatgcgacgt aaaaactaca aattacgggc gatatacctt
     6121 cacattgtta aagccttgct cgcgcagata gagcgcctgc agacggctca tcaccccgcg
     6181 ctcacaccac agcagccagg ttttgttctg gtcgagatcg ccaaatttgg tgctcagttt
     6241 atagaacggc agagaaacca catcaatccc ttcgactttc agtggcttat cttcctgttc
     6301 atcgacagaa cggatatcga gaatcacgtc gttcgggccg aagccgttga cggtttccac
     6361 ttccaccact tcctgctcgg tctgctgggc gatttcgcgg atatcaacgt tattcgcttc
     6421 ctcaaccact ttatcgagaa tactgaagtc gaacttctct tcttccgctt caatcttcga
     6481 tttaaccgct ttcaccgtcg ggcttttgga gatcacgcca caatattccg gcatcgtgcg
     6541 agcaaagtct tcggtgccaa tctggcgagc caggttgatg atgtgctctt tgtcgtaaga
     6601 aatcagcgga cgcaggatca gcgtgtcaga gacgttatca atcaggcgca ggttggtcag
     6661 cgtctgactg gacacctggc cgagcgcttc gccggtgacc agcgcctgta cgccgtaacg
     6721 ttcagccact ttagatgcgg cacgcaccat catacgtttg aggataacgc ccatctgacc
     6781 gtcgtcgatt ttctcgagaa tttccccgac aaccggttcg aaattaatgg cgacaaaacg
     6841 cacgcggtgg gagctgccaa agcggttcca cagataatgc gccacctgac gcacgccaat
     6901 ttcatgcgct gcgccgccga ggttaaagaa gcagtaatgc acgcggcagc cgcgacgcat
     6961 caacatataa ctggaaacac cggagtcgaa accaccggaa atgagcgaca gcacatcttc
     7021 ctgggtgcca atcgggaaac caccaatacc ttcgtagcga cctttaatca gcaggaggcg
     7081 atcgtcttcc acttccagat gaacagttac atccgggtta gtcagcttca cgcgcgcgga
     7141 ttcaatatgc tgatttaaac cgccgccgac gtaacgttcc acatcaatcg agctaaaatc
     7201 atgtttgcca cggcgcttca cgcgtacgca gaaggttttg ccttccagct gatcgcgata
     7261 ctgaaccaac gctttctcaa aaatatcgtg catgtcggta aacggcacgt cttcgacttc
     7321 gagaatatgg tggatacccg gaatacgggt cagggcgtcg cgaatagtca gacgctggtt
     7381 ttcatctttc gcgcgaactt cgatgttatc ccagtggcgg acgacagcga gcgtctcatc
     7441 atagtgcttt aaaacgttac gaatgttccc ggtaaggatt tttataaagc gcaagcgcac
     7501 agattggctt ttgatggtga tttccgggaa caatttaatg ataaacttca tggcggcaat
     7561 ggttcgttgg caagccttaa gcgacttgta tagggaaaaa tacagcagcc cacacctgcg
     7621 gctgcatcca ggcgcggaag tataccacta acatcgcttt gctgcgcaca tcaccttacc
     7681 attgcgcgtt atttgctatt tgccctgagt ccgttaccat gacggggcga aaaatattga
     7741 gagtcagaca ttcattatgc cgaagaaaaa tgaggcgccc gccagctttg aaaaggcgct
     7801 gagcgagctg gaacagattg taacccgtct ggaaagtggc gacctgccgc tggaagaggc
     7861 gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag gggcaggcca aattacaaca
     7921 agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa gacgcctctc taaccccttt
     7981 tacaccggac aatgagtaat ggactttccg cagcaactcg aagcctgcgt taagcaggcc
     8041 aaccaggcgc tgagccgttt tatcgcccca ctgccctttc agaacactcc cgtggtcgaa
     8101 accatgcagt atggcgcatt attaggtggt aagcgcctgc gacctttcct ggtttatgcc
     8161 accggtcata tgtttggcgt tagcacaaac acgctggacg cacccgctgc tgccgtagag
     8221 tgtatccacg cttactcatt aattcatgat gatttaccgg cgatggatga tgacgatctg
     8281 cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag caaacgcgat tctcgctggc
     8341 gacgctttac aaacgctggc gttctcgatt ctaagcgatg ccaatatgcc ggaagtgtcg
     8401 gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg ccagcggtat tgccggaatg
     8461 tgcggtggtc aggcattaga tttagacgcg gaaggcaaac acgtacctct ggacgcgctt
     8521 gagcgtattc atcgtcataa aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca
     8581 ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac tcgacaagta cgcagagagc
     8641 atcggccttg ccttccaggt tcaggatgac atcctggatg tggtaggaga tactgcaacg
     8701 ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg
     8761 ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg acgatgcccg tcaggcgctg
     8821 aaacaactgg ctgaacagtc actcgatacc tcggcactgg aagcgctagc ggactacatc
     8881 atccagcgta ataaataaac aataagtatt aataggcccc tgatgagttt tgatattgcc
     8941 aaatacccga ccctggcact ggtcgactcc acccaggagt tacgactgtt gccgaaagag
     9001 agtttaccga aactctgcga cgaactgcgc cgctatttac tcgacagcgt gagccgttcc
     9061 agcgggcact tcgcctccgg gctgggcacg gtcgaactga ccgtggcgct gcactatgtc
     9121 tacaacaccc catttgacca attgatttgg gatgtgggac atcaggctta tccgcataaa
     9181 attttgaccg gacgccgcga caaaatcggc accatccgtc agaaaggcgg tctgcacccg
     9241 ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg tcgggcattc atcaacctcc
     9301 atcagtgccg gaattggtat tgcggttgct gccgagaaag aaggcaaaaa ttgccgcacc
     9361 gtctgtgtga ttggcgacgg cgcgattacc gctggcatgg cgtttgaagc gatgaatcac
     9421 gcgggcgata ttcgccctga tatgctggtg gtcctcaacg acaatgaaat gtcgatttcc
     9481 gaaaatgttg gcgcgcttaa caaccatctg gctcagctgc tttccggtaa gctttactct
     9541 tcactgcgcg aaggcgggaa aaaagttttc tctggcgtgc cgcccattaa agagctgctc
     9601 aaacgtaccg aagaacatat taaaggcatg gtggtgcctg gcacgttgtt tgaagagctg
     9661 ggatttaact acatcggccc ggtggacggt cacgatgtgc tggggcttat caccacgcta
     9721 aagaacatgc gcgacctgaa aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt
     9781 ggttatgaac cggcagaaaa agacccgatc actttccacg ccgtgcctaa atttgatccc
     9841 tccagcggtt gtttgccgaa aagtagcggc ggtttgccga gctattcaaa aatctttggc
     9901 gactggttgt gcgaaacggc agcgaaagac aacaagctga tggcgattac tccggcgatg
     9961 cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc cggatcgtta cttcgacgtg
    10021 gctattgccg agcaacacgc agtgaccttt gccgccggtc tggcgattgg tggatacaaa
    10081 cccattgtcg cgatctactc caccttcctg caacgcgcct atgatcaggt gctgcatgac
    10141 gtggcaattc aaaagctacc ggtcctgttc gccattgacc gcgcgggcat tgttggtgct
    10201 gacggtcaaa ctcaccaggg cgcttttgat ctctcttacc tgcgctgcat accagaaatg
    10261 gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga tgctctatac cggctatcac
    10321 tataacgatg gcccgtccgc ggtgcgctac ccgcgcggca acgcggttgg cgtggaactg
    10381 acgccgctgg aaaaactacc aattggcaaa ggcattgtga agcgtcgtgg cgagaaactg
    10441 gtgatcctta actttggtac gctgatgcca gaaacggcca aagtcgccga atcgctgaac
    10501 gctacgctgg tcgatatgcg ttttgtgaaa ccacttgatg aagcgttaat tctggaaatg
    10561 gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg ccattatggg cggcgcaggt
    10621 agcggagtga acgaagtgct gatggcccat cgtaaaccag tacccgtgct gaacattggc
    10681 ctgccggact tctttattcc gcaaggaact caggaagaaa tgcgcgccga actcggcctc
    10741 gatgccgccg gtatggaagc caaaatcaag gcctggctgg cataatccct actccactcc
    10801 tgctatgctt aagaaattat tcatggcagg agtggcagca tgcaatacaa ccccttagga
    10861 aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta tgacctttgg cgagccagat
    10921 cgcggtaatc acgcatggac actgccggaa gagagcagcc gtcccataat taaacgcgcg
    10981 ctggaaggtg gcataaattt ctttgatacc gccaatagct attccgatgg tagcagcgaa
    11041 gagatcgttg gtcgcgcact gcgggatttc gcccgtcgtg aagacgtagt cgttgccacc
    11101 aaagtgttcc atcgcgttgg tgatttaccg gaaggattat cccgtgcaca aattttgcgc
    11161 tctatcaacg acagcctgcg acgtctcggc atggattatg tcgatatcct gcaaattcat
    11221 cgctgggatt acaacacgcc gatcgaagag acgctggaag ccctgaacga cgtggtaaaa
    11281 gccgggaaag cgcgttatat cggcgcgtca tccatgcacg cttcgcagtt tgctcaggcg
    11341 ctggaactac aaaaacagca ctgctgggcg cagtttgtca gtatgcagga tcactataat
    11401 ctgatttatc gcgaagaaga gcgcgagatg ctgcctctgt gttatcagga gggcgtggcg
    11461 gtgattccgt ggagcccgct ggcgcggggg cgactgacgc gtccatgggg agaaactacc
    11521 gcacgactgg tgtctgatga ggtggggaaa aatctctata aagaaagcga tgaaaatgac
    11581 gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac tcggtgcaac acgagcacaa
    11641 gttgcgctgg cctggttgtt aagtaaaccg ggcattgccg caccgattat cggaacttcg
    11701 cgggaagaac agcttgatga gctgctgaac gcggtggata tcactttgaa gccataacag
    11761 attgccgagc tggaaacgcc gtataaaccg catgcggtcg taggatttaa ataagagtcc
    11821 aggcctgatg agacgtgaca agcgtcacat caggcatcgg tgcacaacta cgacagaata
    11881 cccagcggcc agtgatgacc gataaagtac aggatgcctg cggaaatcac cccggcgaca
    11941 atatcgtcga tcatgatccc catgccgcca tgtacattgc gatcaaacca gcggatcggc
    12001 cacggcttcc acatatccag aatacggaaa atcacaaacc cggcggcaac ccactgccag
    12061 tcattggtcg gcagcgccat gagcgtgatc cacataccaa taaattcgtc ccagacaatg
    12121 ctgccgtgat cgtgcacgcc catatctttc gccgtttgat gacaaagata gacgccgata
    12181 cagatcccca acatcaccac cagcgagtag agctgccagg gtaaaaaggt catcagatac
    12241 cagaaaggga tcgccgccag cgatcccatc gtgccaggaa cgattgggct taatccactt
    12301 ccgaatccga cagcaagcag atgccacgga ttactcatct tcaggcgact tttcgcgaca
    12361 tctttatggc gtgacaaaat ggtcatatcc tttccagtca aacgtgacag gttcgccgtc
    12421 acgaataaaa caaagccctt cgatatcggc ggtcatttgc ccgatacagg taaacggtac
    12481 gccaaggtgt ccgagagcaa catccagcgc gccacggttc agttccggca ccgtgaaaca
    12541 caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc gcctgttccg gttcaacatg
    12601 gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct
    12661 ggctttcacg atatgcccga gatcggaaat cagaccgtca gagagatcga tagctgaatt
    12721 tgccagatcg cgcagtgcct gcccctgtaa aatacgcggc gatggacgga gatgacgttt
    12781 gatcaagtag tcagcatctt tagcatcggc aacctgcaaa cggttttgca aaatcgccag
    12841 cccggcggcg ctatcgcccg gtgtaccggt cacatagatc cagtcacccg gtttcgcccc
    12901 agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg atacccaacg tcattgataa
    12961 tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg taataattga gaagatcaaa
    13021 caaactgtcg ctgaaggact caagccacgc ttcgtctacg tccggtaagg ttaatgccag
    13081 cgtcagccag gccggatcgg cccccatcgc tgccagatcg cttaggttca ccgccagtgc
    13141 tttataagcc agatcagcag gatcgatatc agggaggaaa tggtttcccg ccaccagcgt
    13201 atcagtgctg atcgccaggg tctgtttctc ggggatattg agaagagcgc aatcgtcgcc
    13261 gatgcccagt tcgacatcaa gacgagaact tcttacacgg tcaaaataac gggcaatcag
    13321 ggagaactcg ccacatgcca tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga
    13381 tcatggaaca gtcttccgtg aatctaccgg cctggatatt actttttgtt agggcgaatc
    13441 acaggtgctg ctttatcgag tacgccgttg acgaacttat ggctgtcttc tgcgccgaac
    13501 gatttcgcca gttcgatcgc ttcgttaatg gccactttgt atggcacatc actacgttta
    13561 gacagttcgt acagcgcaat gcgcagtact gctttttcta cctggcccag ttcttccagc
    13621 aggcgggaca ggtagggctt catcagtccg tcgaggtatg cggtgttagt cgccaccccg
    13681 gccagcagct cacggaagta caggacgtca acgtctttta catcctgttc agccaggaac
    13741 tggtattcaa catcagcgat gtcgttctgg gacaactgcc aggagtagag cgcctggacg
    13801 gcacactcac gagcgcggcg acgagcagca ggtttcacgg atttcccctt actaatttca
    13861 ggccttgatg gctttcaata cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc
    13921 tttgttgcca gctttggtgc cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag
    13981 aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc
    14041 accaccagcg acatattcaa agtgggcagt gccaccacga ataaccgtac ccagcgcaat
    14101 caccgcgtcg tatttaccgg ttttagccag tgcacccgcc gccagcggca gctcataggc
    14161 accaggcacc caaacaacgg taatgttttc atcttttacc tgaccgatac gtttcagtgc
    14221 gtcaattgca ccttccagca ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat
    14281 ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata atgttcattt tcttccttcg
    14341 ggttcgagta tggccccgca ggggggcgga ttttagcata atatttcgtg cgctgcttcc
    14401 ctttcgagcc gggagatcat gcacccacta aatgcaggca aacatccggg cctacatgac
    14461 gtatctcttt gaatttaaat tggggggcgt cggctaattt ctcaagccct ggcagcgagc
    14521 ataatccacg ggcgtcgctg cctaatagtt taggtgcgat atagacaatc agctcatcga
    14581 ctaaacccgc ctgtagcaat acgccagcga gcgttggccc cgcttccacc cagatgctgt
    14641 taatctgctg tttacccagt tgcatcatca gtacaaccag atccagatga cctttatgct
    14701 ctggaatcag caaggtacgc accgtttccg gccactcacg agaatcttcc tgcgtacgcg
    14761 cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc
    14821 tatcaatcac aatacgtact ggctggcgga gattttgttg cgggtagagc gcctgagttt
    14881 gttcatccag ttcagaccaa cgcactgtta aggctggatc atccgccagc accgtggcgc
    14941 tgctggttaa aatggcatga ctttgtgcgc gctgtcgttg tacgtcgcgc cgcgcctgag
    15001 gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt gcggccatca agcgatgcgc
    15061 caagttttaa ctgaatataa ggaaagccgg tgcgcatccg cttgagaaag cctttattca
    15121 attgctcggc ttcactcatc atcagcccgt ggctgacgtc aatgccagcc tgttgcagac
    15181 ggtaaagtcc acgcccagcg acctgcgggt taggatcttg catcgctgcg accacgcgcg
    15241 ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt acggccatga tggctacagg
    15301 gttcgagtgt cacatacgcc gttgcgcctt tggctttttc acccgccata cgcaacgcgt
    15361 gtacttcggc atgtggttca cccgctcgtt ggtggtaacc ttcaccgaca atttcgccat
    15421 ctttgacaat gacgcacccg acattcgggt tggggtgggt ggtaaaacgt cctcgttgcg
    15481 ccagctttag cgcccgcgcc atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg
    15541 cgatctcttc gccaaattct ttgatatctt cgaaactgcg gtagacagag gcaaaacgga
    15601 tataggcgac tttatcgagc tttttcaatt gctccatcac cagattgcca atcatcttgc
    15661 tcggtacttc gcgctcaccg gtggcgcgca gctgcgattt aatatgattg attgccattt
    15721 caacgtaatc ggaactcacc ggacgttttt ccagcgcccg cagcattccg ctacgcaatt
    15781 tctcttcatt aaacggttca cgcacgtcgt tgctttttac aacacgcggc ataaccagct
    15841 ccgccacttc aaaggtggtg aaacgttcat tacacaccag acactgccga cggcggcgta
    15901 cggatgaacc ctcgcccacg agacgagagt caattacctt agtgtccacg gcgaaacaga
    15961 atgggcaatg catacggcgt cctgaccagg tggttaacgg aaatctattt taccctgaac
    16021 tgacgataca acaaaggcgc aacgcttttg agataaagga tcgatgcttt cgctgctttt
    16081 accctctacc attaggctaa ttgcgacgag aaagggacga cctatgaata ctaacgtttt
    16141 tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt
    16201 gcgacagatg aaacacagcg tcagcacgct gaaccaggag atgacgcaac tcaatcaaga
    16261 aacagtcaaa atcactcagc aaaacaggct gaatgcaaaa tccagcagtg gggtttacct
    16321 tctgcctgga gcgaaaacac cggcaagact ggaaagccag atcggtactt tacgtatgtc
    16381 gctggtgaat attacgcctg atacagatgg caccaccctt acactacgta ttcagggtga
    16441 gtcgaatgat cccttgcccg cattcagcgg aactgttgaa tatgggcaaa ttcagggaac
    16501 aatagacaat tttcaggaaa tcaatgtgca gaatcaattg attaatgccc ctgccagcgt
    16561 cctcgccccc agcgatgttg atattccgtt acaattaaag gggatttctg tggatcagtt
    16621 aggctttgta cgtatccacg acattcaacc tgttatgcag taaacgtatt tcgggacgat
    16681 tttgtgcgtc ccgcaacatc ttccccgtca ttttgttact ctgcttacat cacctggatt
    16741 gatagtaaaa gtttgcaaca agggcgaaag tcagtacaat ccccgcccga atgtgtgtaa
    16801 acgtgaacgc aatcgattac gtaaatgata gaactgtgaa acgaaacata tttttgtgag
    16861 caatgatttt tataataggc tcctctgtat acgaaatatt tagaaacgca atttgcgcct
    16921 ttttcactcc cgcaagggat tttcaaacag tggcatacat atgaaaaaaa cattactggc
    16981 agccggtgcg gtactggcgc tctcttcgtc ttttactgtc aacgcagctg aaaacgacaa
    17041 accgcagtat ctttccgact ggtggcacca gagcgttaac gttgtcggaa gctatcacac
    17101 ccgtttcgga ccgcagatcc gcaacgatac ctaccttgag tacgaagcat tcgctaaaaa
    17161 agactggttc gacttctatg gttatgcgga tgcgccggta ttcttcggcg gtaactccga
    17221 tgctaaaggt atctggaacc acggttctcc gctgtttatg gaaatcgaac cacgtttctc
    17281 catcgacaag ctgaccaata ctgaccttag cttcggtccg ttcaaagagt ggtacttcgc
    17341 gaacaactac atttacgaca tgggtcgtaa taaagatggt cgccagagca cctggtacat
    17401 gggtctgggt accgatatcg acactggcct gccgatgagc ctgtccatga acgtctatgc
    17461 gaaataccag tggcagaact atggcgcagc gaacgaaaac gagtgggacg gttaccgttt
    17521 caaaattaaa tactttgtgc cgattaccga tctgtggggc ggtcagctga gctacatcgg
    17581 cttcaccaac ttcgactggg gttccgattt aggggatgac agcggtaacg caatcaacgg
    17641 tattaagacc cgtactaata actctatcgc ttccagccat attctggctc tgaactacga
    17701 tcactggcac tactctgtcg tagctcgtta ctggcacgac ggtggtcagt ggaacgacga
    17761 tgcagaactg aacttcggca acggcaactt caacgttcgc tctaccggct ggggtggtta
    17821 cctggtagta ggttacaact tctgattatg aaaatgccgg gatttattcc cggcatttct
    17881 gattgttaac aacgtgtgct ttgttcatgc cggatgcggc gtaaacgctt tgtccggccc
    17941 acaaaagcac gtaaattcaa tatattgctg aaataacgta ggcctgataa gcgtagcgca
    18001 tcaggcaatt ttacgtttta atcacttctt cttgttcatc atcgctttca ggtcagcgaa
    18061 tgggttgtac ttcgcttcac cgacatcttt ctgcgcatct tcccctgcga taacggtcgt
    18121 accatactga tccgcttcgg tatatttcga atgctcatga tcgtggcaat agagacacaa
    18181 caattcccag ttactgccat cttccgggtt attggtatgg tcgtgatcaa tgtggtgaac
    18241 ggtaagttca cgcaggttag aataaacaaa ctcgcgggaa cagcgaccgc agacccacgg
    18301 atagattttt aatgcttttt cgcgatagcc gctttctaac cgtgcatagt tttttgggat
    18361 gatagccatg aaaagttacc tggatgaaat gtagggttac gcgcagtttc ccaagatgcg
    18421 gggattttcg caatctccat acatcaaaaa aaatcccgat cttctgaccg ggattttcaa
    18481 catcaacggg aacttgatta aggcagaatt gacggctgat ccgccccttc tttttccact
    18541 ttctgctgca acatgtgttc gcgcttcata cccagtttca gagccaacgc agatgctacg
    18601 tagatggaag atgcagtgcc gatggaaaca ccgataagca tggtcagcga gaagccttcc
    18661 agtaccggac caccgaagag atacagcatc aggataacca tcaaggtagt accggatgtg
    18721 atcaaggtac ggtgcagcgt ctgggtcaag gacacgttaa agatttcgta aggcgtaccg
    18781 cgacggatct tgcggaagtt ttcacgaatt cggtccgata ccacgatact gtcgttaagc
    18841 gagtaaccga taaccgacat caacgatgcc acaatggtca ggtcaatctc gatatggaat
    18901 aacgacaaaa tacccagcgt aatgataacg tcgtgcgtca gcgcaataac cacccctgcc
    18961 gccagtcgcc actcaaagcg gaaacctacg tacacgagga tagacagcag cgctgccatc
    19021 aacgccatcg caccggtttg cgcaaggtca gcccccacgc tcggaccgac gaactcaata
    19081 cgcttcactg ctgcattctg attggtggat tcgttaatca ccttcagaac ttggctgccc
    19141 agcacctgac cgccggtttc gccttcagca ggcggcatac ggaccatgat gtcgtggctg
    19201 ctaccaaagt tttgcagcat tggctcttca aaaccggctt tttgcaatgc atcacgcatt
    19261 acgtcaattt cagccggttt ttcgagcgta atttcgataa ccgtaccacc ggtgaaatcc
    19321 agcccccagt taaagccgcg cacgcccata ataacgatag cagcgattaa cagcagacca
    19381 gagatgccga aagcccagta gtcccagcgc ataaagtcat agactttacg gccgtggttt
    19441 agttgttcaa cagtatattc ctgtgccaca tcgcactcct cagattgaca gcttcttgac
    19501 gcgcttgccg ccatatagca ggtttacgat ggcacgcgta ccgacaatcg cggtaaacat
    19561 cgacgtcgcc acaccgatac cggtagtaat cgcgaaccct ttaattgccc cggtacccac
    19621 tgcgtacagg atgatgactt taatcagcgt ggtgatgttc gcatcgaaga tagaactgaa
    19681 tgcgccacga taaccttcat caattgcctg ttgaacagta cgtccgttgc tcaactcttc
    19741 tttaatacgt tcgttgatca gtacgttcgc atcgaccgct accgcaaggg ttaagacgat
    19801 acccgcgata cctggcatac tcagtgttgc gcctggcaac agcgacataa taccgacgat
    19861 caagatcaag ttggcaatca gagcactggt cgcaatcaga ccaaacttct tatagaagat
    19921 gatcatgaac agaatagaaa ccagcagacc ggcaaggcaa gcctccagcc cctgctcaat
    19981 gttctgcata cccaaggtcg ggccaattgt acgttcttca acaatctgaa tcggcgcgat
    20041 caacgcaccg gcacgcagca gcagtgacag ctgacgggct tcgttcgggt tgttgatgcc
    20101 ggtgatacgg aagctgttac ccagacgaga ctggatgttg gcgatgttaa tcacctcttc
    20161 ctgtttcacc agaaccgcac gaccatttgc atctttctta ccgctgtctt tgtactccac
    20221 aaacagggtt gccatcggtt tgccgatgtt gtccttggtg aagttagaca taatgttgcc
    20281 accagcgcta tcgagcgaga tgttaacctg cggctggttg tattcgtcct ggctggaagt
    20341 ggagtcggtg atatggtcac cagtcagaat tacgcgtttg tacagcacaa ccggctgacc
    20401 ttcgcgggtc tgtttcactt cagagtcgcc cggtacgcga ccggatgccg cagcggcctg
    20461 atcaacattg gtgtttacca gacggaattc cagcgttgcc gtcgcgccca gaatctcttt
    20521 cgcacgcgca gtgtcctgaa taccgggcag ttcaacaacg atacggtcag caccctgacg
    20581 ctgaaccacc ggctccgcca cgccaagttg gtttacacgg ttacgcagga tattaatgtt
    20641 ctgctgcacc gcatattcac gcgcttcact cagacgagca tcgctcatta ccgcacgcag
    20701 ctggttgctg ccctggctgc taatcaccag gtccggatgg cgcttgctca gatacgcaat
    20761 ggcttcatca cgagctttag catcgcggaa agtgatgctc aggccgtagt tgttttcttt
    20821 acgaacagtg gtatacggga tgcccttttc gcgcaggtca ctgcgtaggc tatcgatatt
    20881 ttgttcctgg agtttgccaa gcgcggtgtc catatccact tccatcagga agtgaacgcc
    20941 gccacgcagg tcaaggccga gcttcatcgg ctcagcatga atagctgcca gccagcgcgg
    21001 cgttgccggg gcaaggttaa gcgccacgac gtatttgtca cccataacgc ccattaatgc
    21061 ttcacgagcg cgcaactggg tgtcagtgga gtcgaagcgc gcaagaatag cgccctcttc
    21121 cagtgccaca gacttagcag ttattttttc ttcttgtaac gttttctgga cctggatcag
    21181 cgtttgctca ctggcggcga ctccgcgcgc accagtgatc tgaacagccg gatcctcacc
    21241 aaacaggttg ggaagcgcat acagcagacc gatgacaatc accacgatca gcatgacgta
    21301 cttccacaaa ggataacggt ttaacacggc aattccctta gggaaaaatt ttaattacag
    21361 cgccttcatg gtgcctttcg gcaggacggc agctacgaag tcacgtttaa taactacttc
    21421 agtggtgtca ttcagcgcga tagcaatgta gccgttttcc gctactttgg ttacgcgacc
    21481 aaccaggcca ccgttcgtca gaacttcatc acctttggca atggagtcca tcagcttttt
    21541 gtgttctttg gtgcgcttct gctgtggacg caggatcatg aaatagaaaa tcagaccgaa
    21601 taccaccagc atcaaaatca aagacatcgg gctaccttgc gccggtgcac ccgttgccgc
    21661 taccgcatca gaaataaaaa agctcattaa atttccctca ttattaatat taatcaacgt
    21721 tcaaaggtgg tacttctcgc ccctgacgct ggtaaaaatc agttacgaag ctctctaatt
    21781 taccctcttc aatagccttg cgtaaacccg ccatcaaacg ctggtagtaa cgaaggttat
    21841 gaatggtgtt gagtcgcgcg cctaatattt cgttgcaacg gtcgagatga tgcaagtaag
    21901 cgcgtgaata attgcgacag gtgtagcaat cacactcagg atcgagtggg ccagtatcgc
    21961 tcttatattt cgcattgcgg attttcacca cgccatcggt cacgaacaaa tgaccatttc
    22021 gggcgttgcg ggttggcatt acgcagtcaa acatatcgat accgcgacgt acgccttcaa
    22081 ccaggtcttc cggtttacca acgcccatca ggtaacgcgg tttgtctgcc ggaatttgcg
    22141 gacacacatg ctccagaata cggtgcatat ctgctttcgg ctcacccaca gccagaccgc
    22201 cgacagcgta gccatcaaag ccgatatcta ccagaccttt aacagatata tcacgtaaat
    22261 cttcgtaaat actgccctga attataccaa ataacgcatt tttgttacca agactgtcaa
    22321 aacgctcacg gctacgcttc gcccaacgca gagacatctc catagagcgt tttgcgtaat
    22381 cccagtcagc aggatacggc gtacactcat caaagatcat gacgatatcc gaaccaagat
    22441 cgtactgaat ctccatcgac ttttccgggt cgaggaaaat cgggtcgccg ttgatcgggt
    22501 tacggaagtg aacgccctgt tcggtgattt tacgaatatc accaaggctg aagacctgga
    22561 agccgccgga gtcggtaaga atcggtcctt tccactgcat aaaatcgtgc agatcgccgt
    22621 gcagtttcat gatttcctgg cccgggcgca gccacaggtg gaaggtgttg ccgaggataa
    22681 tttgcgcgcc agtggcttca acttcttccg gcgtcatccc ttttacggtg ccgtaggtgc
    22741 caacaggcat aaaacaaggc gtttccacta cgccacgatc aaagaccagg cggccacggc
    22801 gtgcgcgacc gtcggtggtg tccagttcaa atttcatttt ttctccacta cgtcagaaaa
    22861 acagtccaac gtttaaacca gcgccgcgga attactcccc gacgcgctca ttaattgcct
    22921 gcggattgta cgtgataaac atcgcatcac cgtaactaaa aaagcgatat ttctcttcta
    22981 ccgccgcttt ataggcattc atggtgtgtt gataaccggc aaaggccgaa accagcataa
    23041 tcagcgtcga ctctggcaag tggaagttcg tcaccagcgc atcgaccact ttgtactgga
    23101 agcccggata gataaagatt tgggtatcgt caaagaacgg ttcgataaga tcgttttttg
    23161 ccgcctgtgc cgcgctttcc agcgaacgta ctgaagtggt gccaaccgca atcacccggt
    23221 taccgcgcgc tttcgccgcc agtaccgcgt ctaccacatc ctgcggtact tcagcgtatt
    23281 ccgagtgcat gatgtgatct tcaatggtgt cgacgcgcac cggctggaag gtgcccgcac
    23341 caacgtgcaa cgtcacaaac gccatctcca cgcctttggc gcgtaatttt tccagcaaag
    23401 gctcgtcaaa atgcagacca gcggtcgggg ctgcaaccgc gcccggtttt tcgctataaa
    23461 cggtttgata aagttcgcgg tcagcgtctt cgtccggacg atcgatatac ggcggcagcg
    23521 gcatatggcc gatgctgttg aggatatcca gtaccgagcg ttcatcatta aattcgactt
    23581 caaacagtgc gccgtggcgc gcggtcattg ttgcgttgat actttcgtca tcgcccagca
    23641 acagttctgc gccaggtttt ggcgctttcg aggcgcgaat atgcgcaaga atgcgtttgt
    23701 cgtcgagcat ccgctcaacc agcacttcaa tcttgccgcc gctggcttta cgcccaaaca
    23761 gacgcgccgg gatcacgcgg gtattattaa aaaccagaag atcgccgggg ttgagcttat
    23821 cgagtaaatc ggtgaaagta ccgtgcgtca gcgcgcccgt cggcccgtcc agcgacagta
    23881 aacgacagct actgcgttca ggcatgggat agtgggcaat cagggattcg ggcaattcaa
    23941 aggagaaatc ggtaacgcgc atgacactga ctcagactaa aataagagtc gggtagtcta
    24001 gtgccggggc gctctccctg caacctttac ccccctttgg atagaatata atcgatgaat
    24061 tttttagctc acctgcattt agcccatctc gcggaaagct cgctttctgg caatttactg
    24121 gctgatttcg tacgcggaaa tcctgaagaa agttttccgc ccgacgtcgt ggctggcatt
    24181 catatgcatc gacgtattga ctgacaatct gccggaagtc cgcgaagcac aggagtggtt
    24241 tcgtagtgaa acgcgccgcg ttgcgcctat tacgctggat gtcatgtggg atcactttct
    24301 ttcccgccac tggtcgcagc tgtcgccgga ctttccgcta caggaatttg tctgttatgc
    24361 ccgcgagcaa gtgatgacga ttttgccgga ctcaccgcca cgttttatca atctgaacaa
    24421 ttacttgtgg tcagagcagt ggctggtgcg ctatcgcgat atggatttca tccagaacgt
    24481 gttaaacggc atggcaagcc gccgcccacg tctggatgcc ctgcgtgact cctggtacga
    24541 tttagacgct cattatgacg ccctcgaaac ccgcttctgg cagttttatc cgcggatgat
    24601 ggcgcaggcg tcacacaagg cgttataaaa cgttagttca tccataccgt agccgaaatg
    24661 gcaggcaaag ccagaatccc gtcagtcagt tgcccatgcc cttctttgca ttgccattgc
    24721 acggcattga gaaacggtga cgcgggtagc accacttcac tggcctcgcc acggttgatt
    24781 gccaccagta cacgttgctg attcagcacg cggacaaata ccactacgtt atcttccgca
    24841 tacagcacct gacagccgcc acgacgcagc gcctgacttt tcttacgcag cacaatcatt
    24901 cgccggtaca gcgcgaataa cgccgtatcc tgcttttcca cctgccaggg gaacggttta
    24961 cggcaaaacg gatcgttttt accatccagt cccacttcat caccgtaata aatgcacggt
    25021 acaccaggcc aggtgaacag ccagaccacc gccagcggca ggcgcgcaat atcccgaccg
    25081 agcagcgttt taaatcgcgc agtatcgtgg ctatcgagct gattaaacat acgtaattgt
    25141 tgttgatgag aaagccctgc gcggtaatta tccatccagg ccatacaggt ttgggcgtca
    25201 atttgctgcg gatcataaga gatgtcggta ttggcaagaa atccccacaa cgggaatgtg
    25261 aagccacgat agttcatggc agcatcttcc acatcggcct gtaaccattg ccgtgcatcg
    25321 ccaaaatgtt cgccgacaat atacgcttcc ggctgggttt ctttcgccgc ttcggtgatc
    25381 ccggcaacgt gctgcatatt attgcgcgcc ccacccgcct cccccagcat atgcaccaca
    25441 tccagccgcc agccgtccat attccacggc gctttcagcc agtggcggac aatactgtct
    25501 tccccgcgat aaatttcatt caccagactt tccgactgat aatccagctt cgtcaagctg
    25561 gcatagccaa gccagtcgag cgccgtgcca tcatcactaa acgagtacca gtcgcgccag
    25621 ggcgattcag agttgtgaca agctcccccc gtgccaagat tgtgcctgtc aaaccaggca
    25681 tgggaatcgc cactgtggtt aaacacgccg tccagcacca gccgcattcc cagctgctgc
    25741 gtattgtgtc gcaaacgcag caacacccca tcaccgccaa actgcggatc gacatggcga
    25801 taatcctcgg tatcgtattt atgtacgctg ggagctttaa acaccggatt gagatacagc
    25861 gctgtcacgc caagcttttt cagatacggc agtttttcgc ttatcccgtc cagatcgccg
    25921 ccatagaacg ttgatccgcc cgcctgcgcc gtgaccggtt catcccagtc acgcaagatg
    25981 atctcttgtc cggctgcatg atggtaatag acatgatcct gttcagcttc acgaggaaga
    26041 ctacgcgcaa aacgatcagg gaagatctga taaaaaatct gatccgcagc ccattgtggg
    26101 ccgatatcag gtacatcgac ggcaaactgc tccagtcgtg ccggcggcat tcggctgaag
    26161 ccctgcggtg taaaccaacg ctggcgatcg tgccacagca gtttgaaact gtaacgccgc
    26221 cggggttgtc cgctggagag atcaatcgcc gcacgccatg cggtgacgcc tggctgcggc
    26281 tgactgcgct gcttatgcat tggtactgac atttcttcgt tatcgtgttc tgtacgcagc
    26341 ataatgcgct gcggtgggtc ttcgcccgtc agccacagtg taatgagcag ttgatctttg
    26401 ctttgtttaa caaatggggg caccggcagg tgccatgcat ttaacatcat aattcccctg
    26461 tgatgaaaat ctgatcacct tgccatagcc taatgcagga tcactcatca tctgaaggat
    26521 atttggggga ggaggacggg ggcggataac tttcggaata gccggatcag gatttcctcc
    26581 tgaaccggct tacagaacgc aagtattatt ggttttcagc cagctgacga tcgtggcggc
    26641 gtttaaacat ccagccaatc aacagcacaa caatccacgc gaaaccgaca tacagcgaga
    26701 tacgcgtatc cgggtgataa ccaatcaacc cgataataaa gagcaggaaa atcagcccgc
    26761 cgatggtcgt tgctacccca cccggcactt taaatttcag cgccttaact tcttctggcg
    26821 gcaaacggcg acggaaggcg atttgcgaca gcaggatcat aatccacacc cataccgtgg
    26881 cgaaggttgc cagcgaagcg atcaccagga agacgttttc cggcatgatg tagttcagat
    26941 acaccgcaaa cagcagcgcg gtagtcatca ccagcaccgt aacccacgga ataccgcgac
    27001 gcgacgtttt gctgaaaatt ttcggtgcgc tgccctgctc tgccataccg tggagcatac
    27061 ggcctacgcc aaatacgtca ctgttaattg ccgacagcga agcggtcagc acaacaaagt
    27121 taagaatgct ggcggcaaag gtaatgccca tatgctggaa cgtcagtacg aacgggctac
    27181 cggcagtgcc aacctgattc cacgggtaga tagacataat gacgaacagc gtaccgacgt
    27241 agaacaccag aatacgcatc ggcacggagt taatcgcacg cggaatcgat ttctcaggat
    27301 ctttcgcttc accggcggta atcccgataa tttcgatccc accgtaagca aacatcacca
    27361 tttgcaacga cattaccatg ccaagccagc cgttactgaa gaagccgccg ttgctccaca
    27421 ggttatgaat accggtcggt tgcccgccgt tgccaatccc ccagatgatg atgccgaaac
    27481 cggcgacaat catgatgatg atggtggcga ctttaaagaa cgagaaccag aattccagct
    27541 caccgaagac cttcacgctc atcaggttta cggcgcaaat gatcagcacc acgctcagta
    27601 cccaaatcca gtgcggcacc gtcgggaaca agacacccat atagatacca aaagcggtca
    27661 catcggcgat ggcgacaata aggatttcaa aacagtaggt ccatccggta atgtaacctg
    27721 ccagcgggcc aaggttttcc tgcgcataac gcgagaaaga gctggcggcc gggttatgta
    27781 ccgacatttc ccccagcgca cgcataatga tatacgccgc gataccaccg ataatatagg
    27841 ccaacaacac gctcggaccg gccattttga tggcgtctgc cgaaccgtaa aacagcccgg
    27901 tgccaattgc tgaacccagt gccataaagc gtatgtggcg ggtacttagc ccacgcttta
    27961 gcttattctt actttccatc aattcctaac ccatcaacac aataaaaaac cacggggcat
    28021 cgcagccccg tggttaaaac aaatgttcag tgatttagtg agcgctggag gtcacctgac
    28081 gacctgccgc gcgatcccag ataatggcca gaaccaccat caccactgtt ggcattaacc
    28141 acgccagacc ttgttcggcc agcggtaaac gctgcgccca ggacggtaag atatcgctga
    28201 atgcagatgc tttgatcccg tcgagaatac caaaaagcag gctgataaac atcggcggag
    28261 caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa acttaataca accagtgcga
    28321 tacacggcgg ataaatagcg gtcagcaccg gtacggagat ctggatcagc tggcttaagc
    28381 cgaggttaga caccaccatc gagaagccgc cgaggataaa caccagcgta cgataagaga
    28441 gcggtacgta ctgggcaaag aattctgcac aagcacaggt caggccaacc gccgtgacca
    28501 gacaggcgat gaagattaac gccgccagca ggaagctacc gccgccgcca aaggtgtgct
    28561 gaacgtaagc atgcagaata gcagcgccgt ttgcagactg atcgaccagc gacgcgctgt
    28621 ctgaacccag acagaacagc gccaggtaca gcaaagtcag accaacaccc gccatcaggc
    28681 cagcccagac ggtataacgg gtcagcagac gcgcttcggt aacgccacga gaacgcgccg
    28741 cgttaacaat aacgatacca aacaccattg cgcccagcgt atccatggtc agatagccgt
    28801 taacgaagcc gttagaaaat gcagcgtttt gataagcctc agtcgccgtg ctgatagaac
    28861 ccgccggcca gacaatcgcg acaacagaca ggatgaccag cgcgataatt ttcagcggcg
    28921 caaggaagtt gcccacggta tccagcagct tgcccggata gagcgaaacc agaataacga
    28981 tagcgaaata gaccaggctg tagataaaca gcggcagcgc ggaatcaccc gtcagcggcg
    29041 caatccccac ttcaaaggaa acggtagctg tacgcggcgt agcgaaaagc ggccccaccg
    29101 ccaggtaaca aaccgttgcc agcagtacgc cagcgacttt accgattggc gtgctgaggc
    29161 tgtcaacacc gccgccaact tttgccagcg ccactaccgt taataccggc aggccaacgg
    29221 cagtaatgag gaagccgaat gccgcagtcc agacgtgttc gcctgcctgt aagccgacca
    29281 ttggagggaa aataatgtta cctgcgccga cgaacaacgc aaatgtcata aagcccagag
    29341 cgatgatatc gcgcgatctt aattgatggg tcataaatca tactgcctgt ggatgtggtg
    29401 ttgtaacgtt aaatttttgt gccaatccct ggggatgata cagcggtata tttaaacaat
    29461 tttagcggga aatactccca accaggcgcg gcgaagaata gtttttcgat ttaccgcgtt
    29521 aatgcagtca gtatgacagc acctgaagcg caatttaaac gcttataacg ttttaaagca
    29581 aggagggatc taaaaaccag aggtatatag cgataagaaa tcaccaacca gtataaaaca
    29641 ctatttccgt gaaaaagaca tggctaatca tgcgaacaaa aaatcaccaa ccgttgtaaa
    29701 atctaacgcc cgtattttga ctgcaaagaa aataagccag catcggctgg cttatggcaa
    29761 taaaagatga caaaggcgga ttaatcgctg tttttggcaa ttaaacgttc cgggataaca
    29821 aaactgaaac gtgttccttt tcctactgta ctctcaatat tcaggcgact ttcgtgatga
    29881 ttcaccgcat gtttcacgat cgctaaccct aatccgctac cgccggtttg ccgggaacgc
    29941 gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa tatgctccgg tgcaatgccc
    30001 ggtccgttat cttcaacgct aaattcggca ccgtgcggca ctcgctgcca gcgtacggtg
    30061 atatgcgtgc cttccggcgt atgattcacg gcgttataga ccaggttcga aatcgcactg
    30121 cgtagctgat cttcgttgcc agacaccttg aggccgttat ctatctcaaa ggtaaatgtc
    30181 tgttttttct gactcagagt ctgagcctcg cgctcaacaa cgcgcagcat catcggcaca
    30241 tcaacctttt cattgagcaa ttgcgtcggc gcggcttcaa ttttcgacag cgttagcaac
    30301 tgcttcacca gcccttccat ccgctgggtc tgctcgcgca tggtgtgcaa cgctttttcg
    30361 cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca ggtaaccctg taacacggtc
    30421 aatggcgtac gtaactcatg gctcacgttg gcaaaaaagt tacgccgcgc cccttccagt
    30481 tgatgcattt gcgtgacatc acgcgccacc atcagcaact gtttgtgggt ataaggcatg
    30541 acgcgaattt ccagatgccg cccggtgttg agcaccagat tgagcgggcg agaaaaatca
    30601 cgcgttttca gatattgcgt aaactccggg taacgcagta ggttaaggat gttctgcccg
    30661 ttatcttccg gccagcgcaa accaagaatt tgttgcgcca gaccgttaca ccagaaaata
    30721 ccgccctctt ccgtggtcag caccaccgcg tcgggcagcg actccgcgcc gctacgaaag
    30781 cgtttaatca gattgcccag ttcacggcgg cgttttttat ttcgcagctg catctggtgt
    30841 aagccgtata gtagcggttc ccagctacca cgccccggtg gcggggtcat actgcgatcc
    30901 acccacagcc accatgaaag gcgcaataaa ttccagaaat gccagataag cagtcctgtt
    30961 accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg cacccaggat gaaagccggg
    31021 aggcagcaaa gtagcagctc cagcaccagc cttttccacg acagccgttc cagcacgtaa
    31081 gatactccag ttaagaaatc ataagccctg ctctgcgtcc gatgagcaag gcgttaaaag
    31141 cgggttgaaa aacgatatcc tgtaccgcgc acggtctgca ccatgcggtc atgcccgccg
    31201 ggctccagtg ctttacgcag gcgacgaatg tggacatcga ccgtgcggtc ttccacatac
    31261 acgttagttc cccagacgtg gtttaacagc tgctcgcggc tgtacacgcg ctcaggatgc
    31321 gtcataaaaa agtgcagcag tttaaattct gtcggcccca tctccagcgg ctcttcgccc
    31381 gccatcactc ggtgagatgt cgggtcgaga cttaatccct gcatcttaat cacctcttcc
    31441 accgccattg gcgaaatacg gcgcattacc gctttgattc gcgccaccag ctccttcggc
    31501 gaaaatggct tggtgatata gtcatccgcg ccggtttcaa ggccgcgcac gcgatcttct
    31561 tcttcccctc tggcggtcaa catcaccact ggaatatccc gggtcatcga ctcgcgcttg
    31621 aggtgtttga tgaactggat accggagccg ccaggtaaca tccagtcaag gagaattaaa
    31681 tccggctagg gttcattcag ttgattcaca gcactgtcat aatcttccgc ttcgaccggc
    31741 tgaaagccat tttgttcgag cacgaagcag accatttcgc gaattggagc ttcatcttct
    31801 acgaccagaa tacgtctcgc catgatttgc cctgttgtaa taaataggtt gcgatcatta
    31861 atgcgacgtc attatgcgtc agatttatga cagatttatg aaaagctcgt cgcacatatc
    31921 ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg gcacatctgt ttgggtataa
    31981 tcgcgaccat gctttttcgc cagggaaccg ttatgcgcat ccttcacacc tcagactggc
    32041 atctcggcca gaacttctac agtaaaagcc gcgaagctga acatcaggct tttcttgact
    32101 ggctgctgga gacagcacaa acccatcaga tggatgcgat tattgttgct ggtgatgttt
    32161 tcgataccgg ctcgccgccc agttacgccc gcacgttata caaccgtttt gttgtcaatt
    32221 tacagcaaac tggctgtcat ctggtggtac tggcaggaaa ccatgactcg gtcgccacgc
    32281 tgaatgaatc gcgcgatatc atggcgttcc tcaatacaac cgtggtcgcc agcgccggac
    32341 atgcgccgca aatcttgcct cgtcgcgacg ggacgccagg cgcagtgctg tgccccattc
    32401 cgtttttacg cccgcgtgac attatttcca gccaggcggg gcttaacggt attgaaaaac
    32461 agcagcattt actggcagcg attaccgatt attaccaaca acactatgcc gatgcctgca
    32521 aactgcgcgg cgatcagcct ctgcccatca tcgccacggg acatttaacg accgtgggtt
    32581 ccagtaaaag tgacgccgtg cgtgacattt atattggcac gctggacgcg tttccggcac
    32641 aaaactttcc accagccgac tacatcgcgc tcgggcatat tcaccgcgca cagattattg
    32701 gcggcatgga acatgttcgc tattgcggtt cccccattcc actgagtttt gatgaatgcg
    32761 gtaagagtaa atatgtccat ctggtgacat tttcaaacgg caaattagag agcgtggaaa
    32821 acctgaacgt accggtaact caacccatgg cggtgctgaa aggcgatctg gcgtcgatta
    32881 ccgcacagct ggaacagtgg cgcgatgtat cgcaggagcc acctgtctgg ctggatatcg
    32941 aaatcactac tgatgagtat ctgcatgata ttcagcgcaa aatccaggca ttaaccgaat
    33001 cattgcctgt cgaagtattg ctggtacgtc ggagtcgtga acagcgcgag cgtgtgttag
    33061 ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga agaggtgttc aatcgccgtc
    33121 tggcactgga agaactggat gaatcgcagc agcaacgtct gcagcatctt ttcaccacga
    33181 cgttgcgtac cctcgccgga gaacacgaag catgaaaatt ctcagcctgc gcctgaaaaa
    33241 cctgaactca ttaaaaggcg aatggaagat tgatttcacc cgcgagccgt tcgccagcaa
    33301 cgggctgttt gccattaccg gcccaaccgg tgcggggaaa accaccctgc tggacgccat
    33361 ttgtctggcg ctgtatcacg aaactccgcg tctctctaac gtttcacaat cgcaaaatga
    33421 tctcatgacc cgcgataccg ctgaatgtct ggcggaggtg gagtttgaag tgaaaggtga
    33481 agcgtatcgc gcgttctgga gccagaatcg ggcgcgtaac cagccggacg gtaatttgca
    33541 ggtgcctcgc gtagagctgg cgcgctgcgc cgacggcaaa attctcgccg acaaagtgaa
    33601 agataagctg gaactgacag cgacgttaac cgggctggat tacgggcgct tcacccgttc
    33661 gatgctgctt tcgcaggggc aatttgctgc cttcctgaat gccaaaccca aagaacgcgc
    33721 ggaattgctc gaggagttaa ccggcactga aatctacggg caaatctcgg cgatggtttt
    33781 tgagcagcac aaatcggccc gcactgagct ggagaagctg caagcgcagg ccagcggcgt
    33841 cacgttgctc acgccggaac aagtgcaatc gctgacagcg agtttgcagg tacttactga
    33901 cgaagaaaaa cagttaatta ccgcgcagca gcaagaacaa caatcgctaa actggttaac
    33961 gcgtcaggac gaattgcagc aagaagccag ccgccgtcag caggccttgc aacaggcgtt
    34021 agccgaagaa gaaaaagcgc aacctcaact ggcggcgctt agtctggcac aaccggcacg
    34081 aaatcttcgt ccacactggg aacgcatcgc agaacacagc gcggcgctgg cgcatattcg
    34141 ccagcagatt gaagaagtaa atactcgctt acagagcaca atggcgcttc gcgcgagcat
    34201 tcgccaccac gcggcgaagc agtcagcaga attacagcag cagcaacaaa gcctgaatac
    34261 ctggttacag gaacacgacc gcttccgtca gtggaacaac gaactggcgg gttggcgtgc
    34321 gcagttctcc caacaaacca gcgatcgcga gcatctgcgg caatggcagc aacagttaac
    34381 ccatgctgag caaaaactta atgcgcttgc ggcgatcacg ttgacgttaa ccgccgatga
    34441 agttgctacc gccctggcgc aacatgccga gcaacgccca ctgcgtcagc gcctggtcgc
    34501 gctgcatggg cagattgttc cccaacaaaa acgtctggcg cagttaatcg tcactatcca
    34561 gaatgtcact ctggaacaga cgcaacgtaa tgtcgcactt aacgaaatgc gccagcgtta
    34621 taaagaaaag acgcagcaac ttgccgatgt gaaaaccatt tgcgagcagg aagcgcgcat
    34681 caaaacgctg gaagcccagc gtgcacagtt acaggcgggt cagccttgtc cactttgtgg
    34741 ttccaccagc cacccggcgg tcgaggcgta tcaggcgctg gagcctggtg ttaatcagtc
    34801 tcgattactg gcgctggaaa acgaagttaa aaagctcggc gaagaaggtg cggcgctgcg
    34861 cgggcaactg gatgcattaa cgaagcagct tcagcgcgat gaaaacgaag cgcaaagcct
    34921 ccgacaagat gagcaagcac ttactcaaca atggcaagcc gtcacggcca gcctcaatat
    34981 caccttgcag ccacaggacg atattcaacc gtggctggat gcacaagatg agcacgaacg
    35041 ccagctgcgg ttactcagcc aacgacatga attacaaggg cagattgccg cgcataatca
    35101 gcaaattatc cagtatcaac agcaaattga acaacgtcag cagcagctct taacggcatt
    35161 ggcgggttat gcactgacat tgccacagga agatgaagaa gagagctggt tggcgacacg
    35221 tcagcaagaa gcgcagagct ggcagcaacg ccagaacgaa ttaaccgcgc tgcaaaaccg
    35281 tattcagcag ctgacaccga ttctggaaac gttgccgcaa agtgatgatc tcccgcacag
    35341 cgaagaaact gtggcgctgg ataactggcg gcaggttcat gaacaatgtc tcgcattaca
    35401 cagccagcag cagacgttac agcaacagga tgttctggcg gcgcaaagtc tgcaaaaagc
    35461 ccaagcgcag tttgacaccg cgctacaggc cagcgtcttt gacgatcagc aggcgttcct
    35521 tgcggcgcta atggatgaac aaacactaac gcagctggaa cagctcaagc agaatctgga
    35581 aaaccagcgc cgtcaggcgc aaacgctggt cactcagaca gcagaaacgc tggcacagca
    35641 tcaacaacac cgacctgacg ggttggctct cactgtgacg gtggagcaga ttcagcaaga
    35701 gttagcgcaa actcaccaaa agttgcgtga aaacaccacg agtcaaggcg agattcgcca
    35761 gcagctgaag caggatgcag ataaccgtca gcaacaacaa accttaatgc agcaaattgc
    35821 tcaaatgacg cagcaggttg aggactgggg atatctgaat tcgctaatag gttccaaaga
    35881 gggcgataaa ttccgcaagt ttgcccaggg gctgacgctg gataatttag tccatctcgc
    35941 taatcagcaa cttacccggc tgcacgggcg ctatctgtta cagcgcaaag ccagcgaggc
    36001 gctggaagtc gaggttgttg atacctggca ggcagatgcg gtacgcgata cccgtaccct
    36061 ttccggcggc gaaagtttcc tcgttagtct ggcgctggcg ctggcgcttt cggatctggt
    36121 cagccataaa acacgtattg actcgctgtt ccttgatgaa ggttttggca cgctggatag
    36181 cgaaacgctg gataccgccc ttgatgcgct ggatgccctg aacgccagtg gcaaaaccat
    36241 cggtgtgatt agccacgtag aagcgatgaa agagcgtatt ccggtgcaga tcaaagtgaa
    36301 aaagatcaac ggcctgggct acagcaaact ggaaagtacg tttgcagtga aataactatt
    36361 cagcaggata atgaatacag aggggtgaat tatctcttgg ccttgctggt cgttatcctg
    36421 caagctatca ctttattggc tacggtgatt ggttgccgtt ctggtggttg tgatggtggt
    36481 atgaaaaaag tcattttatc tttggctctg ggcacgtttg gtttggggat ggccgaattt
    36541 ggcattatga gcgtgctcac ggagctggcg cataacgtag gaatttcgat tcctgctgcc
    36601 gggcatatga tctcgtatta tgcactggtg gtggtggtcg gtgcgccaat catcgcactc
    36661 ttttccagcc gctactcact caaacatatc ttgttgtttc tggtggcgtt gtgcgtcatt
    36721 ggcaacgcca tgttcacgct ctcttcgtct tacctgatgc tcgccattgg tcggctggta
    36781 tccggctttc cgcatggcgc attttttggc gtcggagcga tcgtgttatc aaaaattatc
    36841 aaacccggaa aagtcaccgc cgccgtggcg gggatggttt ccgggatgac agtcgccaat
    36901 ttgctgggca ttccgctggg aacgtattta agtcaggaat gctggcgtta caccttttta
    36961 ttgatcgctg tttttaatat tgcggtgatg gcatcggtct atttttgggt gccagatatt
    37021 cgcgacgagg cgaaaggaaa tctgcgcgaa caatttcact ttttgcgcag cccggccccg
    37081 tggttaattt tcgccgccac gatgtttggc aacgcaggtg tgtttgcctg gttcagctac
    37141 gtaaagccat acatgatgtt tatttccggt ttttcggaaa cggcgatgac ctttattatg
    37201 atgttagttg ggctagggat ggtgctggga aatatgctaa gtggcaggat ttcaggacgt
    37261 tattcaccac tgcgcattgc agcagtgact gactttatca ttgtactggc actgctgatg
    37321 ctctttttct gcggcggcat gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg
    37381 ggattatttg ccctttcagc acctctgcaa atattgttac tgcaaaatgc caaaggagga
    37441 gagttattag gtggcgcagg tgggcaaata gcgtttaacc tcggtagcgc cgtcggcgca
    37501 tattgcggag gtatgatgct gacgctgggg ctggcatata attacgtggc gctgcctgcc
    37561 gccctgcttt cgtttgctgc gatgtcatcg ttgctgctgt atggtcgcta taagcgccag
    37621 caagcggcgg atagtccggt gctggcgaaa ccactggggt agattactgt cacggtgcgc
    37681 gttgcttatt gccggatgcg gcgtgaacgc cttatccgcc ctacgcggtt ctggcacatt
    37741 ttgtaggcct gataagacgc ggcaagcgtc gcatcaggca tgatgcgcca attgcctacg
    37801 ttttttactc ttgtggccat aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct
    37861 tcgccttact caccggcgtt tcacattcgc cgccgaagac aaattgttta atcaactgcc
    37921 caaccgtttg atataaacgg tctacattgc tcatcccgcc ccccaggaca atcacatccg
    37981 gatcgagaat attcacgaca tgtgccagcg attttgccag ccgcagctcg tagcgacgca
    38041 atgccagttc cgctaccgga tcgctttctt caaccaggcg gataatttca ctgcctttca
    38101 gcgcatgtcc gctcaaacga cgataatccg tcgcgaatcc cgtgcccgaa ataaaggttt
    38161 caatacaacc ttgtttaccg caataacaag ggacttcctc gcgataacgc agttcgtctt
    38221 cgtccatcca cggtagcgga ttgtgtcccc actcacctgc cgtgccattg ccgccgatat
    38281 gcgtccgccc attgaatgcc acgcccgcgc cgcatcccgt gccgatagtc acggcaaata
    38341 ccgtctgcgc tcccgctgcc gcgccatcta ctgcttctga aaccgccaga cagttggcat
    38401 catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac
    38461 cgttgagcca ggttgaattg acattcttca ccacaccggt gtagggcgaa atcgaacctg
    38521 gaatgcccat acctaccgtt ccgcgctgcc ctgtcgcctg ctccgccata tcaaccaacg
    38581 tggcgatcgt ttcaatagtc tgccggtaat catcacgcgg cgtgggcaga cgatggcggt
    38641 acaactgctc ccctgcatcg cccagtgcaa tcacttcagt tttggtgccg cctaaatcga
    38701 tacctatacg cactgtactc tccttatttt tttcaatatc aatagcgtag agacggacaa
    38761 ccggattggc aatgcaaggc cgccgacaat tcgttatcat gcccgctaaa tttaacgaca
    38821 aggccgtgga aattatcatg ctgtggttca aaaatttaat ggtttaccgt cttagccgcg
    38881 agatttcgct gcgtgcagaa gagatggaaa aacagctagc ctcgatggca tttaccccat
    38941 gcggcagcca ggacatggcg aagatgggct gggttcctcc gatgggatcg cacagcgatg
    39001 cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc gcgcaaagaa gaaaaaatcc
    39061 tcccgtctcc ggtgattaaa caggcgctgg aagcgaaaat cgccaaactg gaagcggaac
    39121 aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa agacgaagtg ctgcactctc
    39181 tgctgccgcg tgctttcagc cgttttagcc agacaatgat gtggctcgac acggttaacg
    39241 gtttgattat ggtggactgc gccagtgcca aaaaagcgga agatacgctg gcattactgc
    39301 gtaaaagcct ggggtcgtta ccggttgtac cattgagcat ggaaaacccg attgaactga
    39361 cgctgaccga atgggttcgc tccggtagtg cggcacaggg cttccagctg cttgatgaag
    39421 ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa caagatctga
    39481 ccagcgaaga gatcaccaat cacattgaag ccggaaaagt ggtgactaaa ctggcgctcg
    39541 actggcagca gcgcattcag tttgtgatgt gcgacgatgg ttcgctcaag cgtcttaagt
    39601 tctgcgacga gctgcgcgat caaaacgaag atatcgaccg tgaagatttc gcccagcgtt
    39661 ttgatgccga tttcatcctg atgactggtg aactggcagc gttaattcaa aacctgattg
    39721 aaggattagg tggcgaagca caacgttaat tgctgatttt cctttaatgc cggatgcgac
    39781 gcctgccgcg tcttatccgg cgtacgaagc cacaccaagc atataattat tcgctacggc
    39841 gagcaataat ttttagcgca gcaatattat gcgttttacg ccgtaactga tctaatcgcc
    39901 tcatccctat tcgcctgtca gcagcacgca gaatattcac taacccccat ttatcgctgg
    39961 taaattgttt tgttaacagc tccgcttttg gggtatcaat atattcacgc aataaatcag
    40021 aaattgaaca aatatccgtc aggtaaggat accagcaagt ttcaccgtga aaattacgca
    40081 ctccagcgtt acctgcaata aagtctttcg ggtaatccca gatcacgttt ttatccaacg
    40141 tgatccaata acgcggtaaa tcggtacttc caccattttg gctgcgcatg ggataacggg
    40201 tacagtgaat ttgcaggttg atcgttggcg tcagtaagtc atagatctcc cgctgtaact
    40261 tgctccatgg acgttgtgtc attgtttttc ctcaagccgg tgacgcgctt cactgcgaat
    40321 tttccacgtt gccccggttt tcactaaatc acgtaactgc gaaagatcgg gttattcgac
    40381 aaataagagt aatgatggat cctcttttaa ccacgccgtt ttcacatcgg cagccagctg
    40441 tggattatta atagccagcg accgatcctg aggctcagtc aatgtcgtta acaacgacgc
    40501 cggggtatgt gcgttgcgta atgccgtttg gcgcaaatga tcattgcgac tttgcagcaa
    40561 tgcacgccac cattcatcat ctgatttatc ttttatcgta gccagttccg caaaaaatac
    40621 ctggagatct tcactgatat ttatatcaac cttgatgaat tatgaatata tcggaaattg
    40681 aaaaaatcgc agcaaaggaa atattattat gggttaaaga gaggatattt cagaaaaatg
    40741 ccctcccgtt caaggggaag gcgaggaatt acagatagcg gcacagataa gaggtaggtt
    40801 cggcaacttg aagatgaaac tcactgtgac cgggaacatt aaacaccgaa ccggcttcat
    40861 acacttgcca gtcggtcgcg tcaggcagta acacattcag cgcgccactg attaccgtca
    40921 tctcttccgg ctcagcggtg ctgaaggtgt attcgccttc aaccataaca cccacgctgg
    40981 cgcgaccagt gctgctgctg gaaaagccga tcgatttcac tttgccggaa aagtactcat
    41041 tactttgaag cataaactgg ccctgtctgg tagttgaaca tctacttcac tataggggcc
    41101 agaggcgctg gctgtcacgc aaaattacac cagtaattcc gcagccagcc gtgcaatcaa
    41161 tacgttagag agcaagacag gaacatcgag ctgtttttgc agtaaatcgc gatgacgttg
    41221 gtgaaatcct aaacaatcca gcatgatgac atcagcccct tttgccagta attctttccc
    41281 agcatcaatg attttttgtt ctgaatcatg aatggggtta cccaatgaaa ataccggcga
    41341 tttctgcaaa atttgccatt tttgcgcctg aacgggcagc atctcctcaa ccggaacgat
    41401 aacccccacc tgatgatctt caacaataga ggaaaccagt ggaggcaata ttcgcgacgg
    41461 ctcaagaaag atcgtattac gcgcagtcat actactaatg tttgctgtac tcattaataa
    41521 aatgacgtca taaccctgat tatcgagcac ttcaaccaca ccttgcagat ctcgctccac
    41581 tttacgacgc gaaacatggg ccagctggtt gtcatttaat aatgtgagaa tggtgtcttc
    41641 gcctgcttct ggcgcgtact ccgccatcac ttcttcacga cttaacttcc ccagcaggct
    41701 atgatgggaa atattatctt cgtcaatgta ttccgtcagg agcggcaaaa cctcctgcat
    41761 aggtacaatg ccgatggtca ggatcgccaa cgacgcactc attgttcatc caccttactt
    41821 ttctttcgtc gttaattacc gggcaagtgt gaagcaccac tgctgacact tacttgtgct
    41881 acaaatgaca aaaagcgtag cagcagacgt gcacggcata atgtaaagat tccaaatgat
    41941 tccagtaatg gaatgttatt gtttaatatt ctaattatta gaaaaacata aattatgaaa
    42001 aatgtgacgc agatcccttg tagtggcact cagaatccct tccgagcagt ctgatgcggt
    42061 cactatttgt cagggtcctc atagcgtttt ttcgcatcca tcgcttcctg agccgtcgga
    42121 tgttcactga tcaacgagtc tggtttagga tgatcggctc tgagttggta ccaggttacc
    42181 gtctgtcctg gctttccttt ttcaatcgtc actatatatg cttcacgagg ataaggcggt
    42241 ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt taagtatagg cgctctaaaa
    42301 tcaacaattg atcgtctgtg ccagggcgct acgaatttca gaaatcacct ggctgggttc
    42361 gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat agcgcatcgc gttcttccag
    42421 cacttcctga acttcttcgc tcagcggttt tcccgttaag gttggccgta aatcttcttc
    42481 cggtgcagct tgcagtcggt taaccaggac tgatactggc gcacacaaat aaaccacgat
    42541 cccgttattt tgcatgaagt gacgattaaa ttccgtcaga ataatgccgc cgcctgtagc
    42601 gataacggtg gatgccgcag ttaccgcttc cagcgccgcc gtttctctgg cgcgaaatcc
    42661 cgcccactct tccctttcga cgatctccgc gaccgtcata ttgagctgtg attgcaacca
    42721 ctgatcggta tcgacaaacc gacggttaag cgaatcggca agggccattc cgaccgttgt
    42781 tttaccacag ccccgaggcc cgatcagaaa aagaggttgt gtcatcgtgg gttttcccca
    42841 ataggtcgca atgcggcgaa agccggtgtc atgagaatag cgatcatacc atcaaactag
    42901 tacaatttcg attgtaaaga aaaaattcca cttaaagtaa aaatctcaat acatccctta
    42961 ctataccaat aaatattcaa gaatgaagtg taaataataa attacattta gccacgacta
    43021 cgttgcactt ccagccacca cttctccagc tccgcggcaa aggcctggcg gtcacgttgt
    43081 gaaaggctat ctggtccgcc ggtctggatc ccactggcac gtaaggtatc cataaaatcg
    43141 cgcatcgtca ggcgctcacg aatggtcgct ggcgtgtaac gttcgccgcg cgaattaagc
    43201 gccgctgcgc ctttctcgat ggcttcagca gccaaaggta tatctgcggt gatcaccaaa
    43261 tcgcccgctt cacactgccg gacaatttcg ttatcggcaa cgtcgaaacc tgccgcgacg
    43321 cgcagcgtac gaataaatcg cgatggcggc acgcgtaaac tctggtttgc taccagtacc
    43381 agcggcatct gcatacgttc cgccgcgaga tacaaaatct ctttaattac attgggacac
    43441 gcgtcggcat ccacccaaat tgtcataaag tcatcctttg ttaggtaatc ctctattgtg
    43501 tcgcgctttt gccttccggc atagttctgt ttatgcttct gccagcgatt atcaaaacaa
    43561 tgaatttcac ggcaggagtg aggcaatgga aaagaaaatc ggttttattg gctgcggcaa
    43621 tatgggaaaa gccattctcg gcggtctgat tgccagcggt caggtgcttc cagggcaaat
    43681 ctgggtatac accccctccc cggataaagt cgccgccctg catgaccagt tcggcatcaa
    43741 cgccgcagaa tcggcgcaag aagtggcgca aatcgccgac atcatttttg ctgccgttaa
    43801 acctggcatc atgattaaag tgcttagcga aatcacctcc agcctgaata aagactctct
    43861 ggtcgtttct attgctgcag gtgtcacgct cgaccagctt gcccgcgcgc tgggccatga
    43921 ccggaaaatt atccgcgcca tgccgaacac tcccgcgctg gttaatgccg ggatgacctc
    43981 cgtaacgcca aacgcgctgg taaccccaga agataccgct gatgtgctga atattttccg
    44041 ctgctttggc gaagcggaag taattgctga gccgatgatc cacccggtgg tcggtgtgag
    44101 cggttcttcg ccagcctacg tatttatgtt tatcgaagcg atggccgacg ccgccgtgct
    44161 gggcgggatg ccacgcgccc aggcgtataa atttgccgct caggcggtaa tgggttccgc
    44221 aaaaatggtg ctggaaacgg gagaacatcc gggggcactg aaagatatgg tctgctcacc
    44281 gggaggcacc accattgaag cggtacgcgt actggaagag aaaggct
//
LOCUS       DAJTUN010000018        43886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTUN010000018 DAJTUN010000000
VERSION     DAJTUN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.018"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO470_000961"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO470_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6250293.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="OO470_000962"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="OO470_000962"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6250294.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3051..4091
                     /locus_tag="OO470_000963"
     CDS             3051..4091
                     /locus_tag="OO470_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6250295.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="OO470_000964"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="OO470_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6250296.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="OO470_000965"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="OO470_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6250297.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7086
                     /gene="lnt"
                     /locus_tag="OO470_000966"
     CDS             5548..7086
                     /gene="lnt"
                     /locus_tag="OO470_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6250298.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7483..8391
                     /gene="gltI"
                     /locus_tag="OO470_000967"
     CDS             7483..8391
                     /gene="gltI"
                     /locus_tag="OO470_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6250299.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8561..9301
                     /gene="gltJ"
                     /locus_tag="OO470_000968"
     CDS             8561..9301
                     /gene="gltJ"
                     /locus_tag="OO470_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6250300.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9301..9975
                     /gene="gltK"
                     /locus_tag="OO470_000969"
     CDS             9301..9975
                     /gene="gltK"
                     /locus_tag="OO470_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6250301.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9975..10700
                     /gene="gltL"
                     /locus_tag="OO470_000970"
     CDS             9975..10700
                     /gene="gltL"
                     /locus_tag="OO470_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6250302.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10818..11753
                     /gene="rihA"
                     /locus_tag="OO470_000971"
     CDS             10818..11753
                     /gene="rihA"
                     /locus_tag="OO470_000971"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6250303.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11837..13498
                     /gene="hscC"
                     /locus_tag="OO470_000972"
                     /pseudo
     CDS             11837..13498
                     /gene="hscC"
                     /locus_tag="OO470_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13558..15009)
                     /gene="djlC"
                     /locus_tag="OO470_000973"
     CDS             complement(13558..15009)
                     /gene="djlC"
                     /locus_tag="OO470_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6250304.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15006..15713)
                     /locus_tag="OO470_000974"
     CDS             complement(15006..15713)
                     /locus_tag="OO470_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6250305.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15815..16369
                     /locus_tag="OO470_000975"
     CDS             15815..16369
                     /locus_tag="OO470_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6250306.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16379..17806)
                     /locus_tag="OO470_000976"
     CDS             complement(16379..17806)
                     /locus_tag="OO470_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6250307.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17803..18510)
                     /locus_tag="OO470_000977"
     CDS             complement(17803..18510)
                     /locus_tag="OO470_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6250308.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18674..19651
                     /locus_tag="OO470_000978"
     CDS             18674..19651
                     /locus_tag="OO470_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6250309.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19721..20203)
                     /locus_tag="OO470_000979"
     CDS             complement(19721..20203)
                     /locus_tag="OO470_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6250310.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20438..23020
                     /gene="leuS"
                     /locus_tag="OO470_000980"
     CDS             20438..23020
                     /gene="leuS"
                     /locus_tag="OO470_000980"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6250311.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23035..23616
                     /gene="lptE"
                     /locus_tag="OO470_000981"
     CDS             23035..23616
                     /gene="lptE"
                     /locus_tag="OO470_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6250312.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23616..24647
                     /gene="holA"
                     /locus_tag="OO470_000982"
     CDS             23616..24647
                     /gene="holA"
                     /locus_tag="OO470_000982"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6250313.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24649..25290
                     /gene="nadD"
                     /locus_tag="OO470_000983"
     CDS             24649..25290
                     /gene="nadD"
                     /locus_tag="OO470_000983"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6250314.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25314..25925
                     /locus_tag="OO470_000984"
     CDS             25314..25925
                     /locus_tag="OO470_000984"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6250315.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26185..26502
                     /gene="rsfS"
                     /locus_tag="OO470_000985"
                     /gene_synonym="rsfA"
     CDS             26185..26502
                     /gene="rsfS"
                     /locus_tag="OO470_000985"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6250316.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26506..26973
                     /gene="rlmH"
                     /locus_tag="OO470_000986"
     CDS             26506..26973
                     /gene="rlmH"
                     /locus_tag="OO470_000986"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6250317.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27004..28905
                     /gene="mrdA"
                     /locus_tag="OO470_000987"
     CDS             27004..28905
                     /gene="mrdA"
                     /locus_tag="OO470_000987"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6250318.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28908..30020
                     /gene="mrdB"
                     /locus_tag="OO470_000988"
                     /gene_synonym="rodA"
     CDS             28908..30020
                     /gene="mrdB"
                     /locus_tag="OO470_000988"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6250319.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30031..31119
                     /gene="rlpA"
                     /locus_tag="OO470_000989"
     CDS             30031..31119
                     /gene="rlpA"
                     /locus_tag="OO470_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6250320.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31258..32469
                     /gene="dacA"
                     /locus_tag="OO470_000990"
     CDS             31258..32469
                     /gene="dacA"
                     /locus_tag="OO470_000990"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6250321.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32580..32843
                     /locus_tag="OO470_000991"
     CDS             32580..32843
                     /locus_tag="OO470_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6250322.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            32944..33585
                     /gene="lipB"
                     /locus_tag="OO470_000992"
     CDS             32944..33585
                     /gene="lipB"
                     /locus_tag="OO470_000992"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6250323.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33844..34797
                     /locus_tag="OO470_000993"
     CDS             33844..34797
                     /locus_tag="OO470_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6250324.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35006..35971
                     /gene="lipA"
                     /locus_tag="OO470_000994"
     CDS             35006..35971
                     /gene="lipA"
                     /locus_tag="OO470_000994"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6250325.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36072..36275)
                     /gene="tatE"
                     /locus_tag="OO470_000995"
     CDS             complement(36072..36275)
                     /gene="tatE"
                     /locus_tag="OO470_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6250326.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36404..37192)
                     /locus_tag="OO470_000996"
     CDS             complement(36404..37192)
                     /locus_tag="OO470_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6250327.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37285..37668
                     /gene="crcB"
                     /locus_tag="OO470_000997"
     CDS             37285..37668
                     /gene="crcB"
                     /locus_tag="OO470_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6250328.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37722..37931)
                     /gene="cspE"
                     /locus_tag="OO470_000998"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37722..37931)
                     /gene="cspE"
                     /locus_tag="OO470_000998"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6250329.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38106..38666)
                     /gene="pagP"
                     /locus_tag="OO470_000999"
     CDS             complement(38106..38666)
                     /gene="pagP"
                     /locus_tag="OO470_000999"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6250330.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39254..40639
                     /gene="dcuC"
                     /locus_tag="OO470_001000"
     CDS             39254..40639
                     /gene="dcuC"
                     /locus_tag="OO470_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6250331.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLDRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(40680..41021)
                     /locus_tag="OO470_001001"
                     /pseudo
     CDS             complement(40680..41021)
                     /locus_tag="OO470_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41359..41868)
                     /locus_tag="OO470_001002"
     CDS             complement(41359..41868)
                     /locus_tag="OO470_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6250332.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42031..42276
                     /locus_tag="OO470_001003"
     CDS             42031..42276
                     /locus_tag="OO470_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6250333.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42251..43882
                     /locus_tag="OO470_001004"
                     /pseudo
     CDS             42251..43882
                     /locus_tag="OO470_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        11050 a        10971 c        10947 g        10918 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
       61 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
      181 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
      241 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
      301 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
      361 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
      421 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
      481 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
      541 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
      601 gccatattca tattctgcaa ctggcgaata cgcgccggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
      841 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac
     1021 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
     1141 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
     1201 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
     2101 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
     2401 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
     2581 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
     2641 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
     2701 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
     2821 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
     3421 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
     3781 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
     3901 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
     4021 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
     4321 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
     4381 ggagcaaggc aaaccactgg aggcgcattg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
     5281 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
     5401 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gcttttgcct cattaattga acgccagcgt
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
     5701 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
     5821 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
     6181 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
     6421 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
     6481 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
     6601 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
     6661 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagatcatt
     6721 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
     6781 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
     6841 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
     6901 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
     6961 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
     7021 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
     7081 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
     7141 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
     7201 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
     7261 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
     7321 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
     7381 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
     7441 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
     7501 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
     7561 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     7621 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
     7681 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     7741 caggtaaaac tgattccgat tacctcacaa aaccgtattc cactgctgca aaacggcact
     7801 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
     7861 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
     7921 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     7981 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     8041 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
     8101 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
     8161 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
     8221 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     8281 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
     8341 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
     8401 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
     8461 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
     8521 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
     8581 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
     8641 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
     8701 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
     8761 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
     8821 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
     8881 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
     8941 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
     9001 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
     9061 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
     9121 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
     9181 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
     9241 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
     9301 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
     9361 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
     9421 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
     9481 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
     9541 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
     9601 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
     9661 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
     9721 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
     9781 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
     9841 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
     9901 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
     9961 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    10021 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    10081 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    10141 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    10201 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    10261 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    10321 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    10381 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    10441 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    10501 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    10561 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    10621 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    10681 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    10741 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    10801 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    10861 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    10921 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    10981 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    11041 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    11101 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    11161 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    11221 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    11281 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    11341 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    11401 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    11461 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    11521 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    11581 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    11641 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    11701 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    11761 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    11821 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    11881 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    11941 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    12001 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    12061 aatgggcagc tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt
    12121 attacgctca ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt
    12181 gatctccgtt ccggcttatt tcagtgatga acaacgcaag catacccgtt tagcagcgga
    12241 cttagccggg ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta
    12301 tggcctgcat acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt
    12361 tgacgttacg gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctggcga
    12421 caacttcctc ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc
    12481 ggatgtcaca aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga
    12541 agcggcaaaa tgtagcaatc aatcgccaat gcacattcgc tggtagtacc aggatgaaac
    12601 gcgggaatgc gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg
    12661 cttgcgagtg ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga
    12721 cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg
    12781 tctgtttggc aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc
    12841 agcaatccag gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga
    12901 tatttgccct tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc
    12961 gccgattatt gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat
    13021 gcacccggaa caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa
    13081 aaacaacatt ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc
    13141 gattgatatt cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga
    13201 agacggcagc gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca
    13261 gattgaagag agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat
    13321 caatcgcacc tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg
    13381 agaagagatt ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc
    13441 ccgcgtcgat gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc
    13501 acggctgccg gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca
    13561 actaaacaac atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat
    13621 tttgataatg ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt
    13681 ttcaggtgtt ggctcatcga catcttcatc ctccggctcg agttttactt tcgtcaacag
    13741 catccgggtg ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag
    13801 acgggccagc atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata
    13861 caaatccagt aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc
    13921 ccatgctttg cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt
    13981 tccgctatgt aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata
    14041 tttcagcggt tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa
    14101 cgcgaaataa tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt
    14161 agatgggtaa ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca
    14221 gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg
    14281 ccactgtttg gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg
    14341 caacagtttg cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa
    14401 aaacgcagtg acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc
    14461 aacagtctgg ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc
    14521 accagcctga atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc
    14581 attaaacgaa tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata
    14641 tcgcgcttct atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt
    14701 ccaggtattt aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc
    14761 tgaatccagt aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc
    14821 ttcatcatct gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg
    14881 gcgtaatgct ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag
    14941 ataagcctgg cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca
    15001 tgttttcata ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat
    15061 ggctgccagg gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc
    15121 cgactcccgg cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct
    15181 tcgcactgag aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc
    15241 cagctgtgat agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct
    15301 tcttccgtca gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa
    15361 tcccaggctt tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta
    15421 taaatcaacc ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag
    15481 cgttgccatg tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga
    15541 taaatagtct gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat
    15601 tcttttatcc cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt
    15661 atttccatgg gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa
    15721 taatccctta agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc
    15781 tttcaaaata aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt
    15841 cgcaattttt atagttgcag caattacctg tattagccaa cccaagaaaa cgatgttgcg
    15901 tgataaagcc atggtgaatt atgcctttga ttatttaaga tcaccaggca gtctaccgtt
    15961 aaccacggca gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct
    16021 tggcgaattt tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata
    16081 ctggtatgag caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tgaggtggat
    16141 ctacctgaaa ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga
    16201 agcagctgaa caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg
    16261 cactggtatt aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca
    16321 acattacaaa aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc
    16381 acccgaagag atggagtatt ttccctatca aaccggcaaa gataaaaatg tagaaaataa
    16441 ttttgatgac gcccagacag cccagcccgc catcatcaac gggcattggc tgtggttgcg
    16501 gctccggctc gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa
    16561 taattaatgc ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta
    16621 atgattcttc tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc
    16681 ataagggatg ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct tcgtgcaaaa
    16741 ccatctcgac cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata
    16801 aatgaaaacg cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctgatcgt
    16861 taaccagatc acgcgcttca atcatcatca ccactaatgg gtaataatag acccgatgct
    16921 gacggcacca ttgcaacatt aaagcatccg cctgggtgga gggatactcg cgccagtagt
    16981 aacaaagttc tgccagcagg ctttcgcctt ctccacaata gacgcgttgt gcgtattcat
    17041 aataacgcgc atcttcattg tcaggttcag cttcccgcca cgaccgggcg taatccagga
    17101 gttcggggat tcccaggcgg cttgcgctat accagcggag taaatttctg tgcagttttg
    17161 catcatcggg gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat
    17221 gcgatgacca aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa
    17281 ctggcagatg cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa
    17341 aggattccag ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc
    17401 tttctgctaa taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc
    17461 acagcgacca gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga
    17521 atcgctgcca ggcggaggac agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg
    17581 cgagcagaat ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag
    17641 gcgactgcgc aatccgtagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg
    17701 gatcggtttc tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga
    17761 cgtcagtcgt ttcctctata tcgaggatct tccagcaatt tttcataagt cgctcatatc
    17821 cttaagcgtt tccgggcatg tggggtagtc agccagtggt tgccaggaca aggtagggta
    17881 atacgcatca tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc
    17941 tttacgcgct aagaattcgc gtaataaagg cagatgatca tcttcgggag actgccaata
    18001 ttgccgacca aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc
    18061 ccgtagatga atacgcgact ggatccatag actttcttct tcactcaacc agttattaag
    18121 tacgcccatt cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc
    18181 accacagcat tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa
    18241 ttcagcaaga tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc
    18301 tgccaaccga gcggcatgac cgttctcgat cattcggtga attatgcgca gcaggtcatc
    18361 acggtcatca attccccatg aggaagaaag gtcaaaaaag catgtaccta aatacatttt
    18421 gggggatgaa tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc
    18481 aaacaatatt ttttgcgacc ccatgtccat ataatttatc cttaaacatt catttaatga
    18541 atgtaaacta catttcgagg ctcagttatt tatacttttc tgacgcaaca taatacgcat
    18601 aaaataaggc ttacgcaaaa atggaataca taaaaatata atatatttta tctacaacca
    18661 taaggaaatg ataatgattt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat
    18721 tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa
    18781 tcgcgatagc gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc
    18841 cgctgagcaa ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa
    18901 aagccgtcat aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg
    18961 ccatactttc gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta
    19021 taaagaagcg gttacctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa
    19081 taatcttgga tggatgtaca gaaatggcaa cggagtcgca caagactata cgctggcatt
    19141 tttctggtac aaacaagctg cattacaagg ccatagttac gcacaagaca atctggccga
    19201 tctttataaa gacggagaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt
    19261 gaaaagcgca cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc
    19321 tggcgaaggg gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc
    19381 tcaggaaagc gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt
    19441 tgagaaagat tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc
    19501 cactgcctgg tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct
    19561 ccgacaggct ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca
    19621 ggaaattcgt gagactgaag ctttactgta gaaataaaaa caagggccgg ggaatttcat
    19681 tctgcccata ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc
    19741 tctggaactg gtcataacca catttcgggc atagcgtcag cacttccggt gtgtagatcg
    19801 ggagatggaa gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt
    19861 gataaacccc atgatgattg aggtcctgga aaatttcgcg ccattcaagc tgcgttttat
    19921 cggtgatgtc tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt
    19981 cagattcttc tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga
    20041 cagctcgcgt cagctcatcg atctcggttc gcgttaactc ccctgttttt attacgcgct
    20101 cgcgcgcctg ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgttca
    20161 gtgacgcgac cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt
    20221 cgttaactga ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa
    20281 ctctgtaaat taatgtacga ataatccggg catcatttca acgtcgagtg cgcgaaaggc
    20341 tgttttgacg cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac
    20401 atttgtagcc gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga
    20461 agagatagaa tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga
    20521 agacgagagc aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact
    20581 acacatgggc cacgtacgta actacaccat cggtgacgtg atcgcccgtt accagcgcat
    20641 gctgggcaaa aacgttctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg
    20701 cgcggcggtg aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat
    20761 gaaaaaccag ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg
    20821 tacgccggaa tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct
    20881 ggtatataaa aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa
    20941 cgaacaagtt atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat
    21001 cccgcagtgg tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa
    21061 actggatcac tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga
    21121 aggcgtggag atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac
    21181 ccgcccggac acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc
    21241 gcagaaagcg gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac
    21301 caaagttgcc gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa
    21361 agctgttcac ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat
    21421 ggagtacggc acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt
    21481 tgcctctaaa tacggcctga acatcaaacc ggttatcctg gcggctgacg gctctgagcc
    21541 agatctttct cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa
    21601 cggtcttgac catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt
    21661 tggcgagcgt aaagtgaact accgcctgcg cgactggggt gtttctcgcc agcgttactg
    21721 gggcgcgccg attccgatgg tgacgctgga agacggtacc gtaatgccaa ccccggacga
    21781 ccagctgccg gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa
    21841 agcagatccg gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga
    21901 cactttcgac acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta
    21961 caaagaaggt atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat
    22021 tggtggtatc gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat
    22081 gcgtgatgca ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat
    22141 ggtgctggca gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc
    22201 ggttgatgct atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc
    22261 aggccatgaa ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat
    22321 cgacccgcag gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt
    22381 tgcttctccg gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg
    22441 cttcctgaaa cgtgtctgga aactggtgta cgagcacaca gcaaaaggtg atgttgcggc
    22501 actgaacgtt gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac
    22561 tatcgctaaa gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc
    22621 gattatggag ctgatgaaca aactggcgaa agcaccgacc gatggcgagc aggatcgcgc
    22681 tctgatgcag gaagcactgc aggccgttgt ccgtatgctc aacccgttca ccccgcacat
    22741 ctgcttcacg ctgtggcagg aactcaaagg cgaaggcgat atcgacaacg cgccgtggcc
    22801 ggttgctgac gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg
    22861 taaagtccgt gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg
    22921 tgctggccag gaacatctgg tagcaaaata tcttgatggc gttactgtac gtaaagtgat
    22981 ttacgtacca ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga
    23041 tatctggcaa cactgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat
    23101 ctgcgtgaca ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat
    23161 ccgaacgggc cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg
    23221 cttgataaag aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc
    23281 gcgaaagata ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg
    23341 acggtgaatg cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc
    23401 ttccgttcat tcttcgataa cccgcaaatg gcgttagcga aagataacga acaagacatg
    23461 atcgtaaaag agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc
    23521 cgtgctgcgg atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca
    23581 acgcctgcac gcgtctccac catgctgggt aactgatgat tcggttgtac ccggaacaac
    23641 tccgcgcgca gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc
    23701 tgttattgca ggaaagccag gacgctgttc gccaggtagc tgcggcacaa ggattcgaag
    23761 aacaccacac tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc
    23821 aggctatgag tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac
    23881 cgaatgcggc gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc
    23941 tattgatcgt ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg
    24001 ctcttgcgaa tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc
    24061 gctgggttgc tgtgcgcgca aaacagctca acttagaact ggatgacgcg gcgaatcagg
    24121 tgctctgcta ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat
    24181 cgctgctctg gccagacggc aaactgacat taccgcgcgt tgaacaggcg gtgaatgatg
    24241 ccgcgcattt cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg
    24301 cgttgcatat tcttcagcaa ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca
    24361 cattacaacg tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac
    24421 tgcgtgcgtt gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg
    24481 cgttaaatcg cttaagtcag ccgcagttac gtcaggccgt gcaactcctg acacgaacgg
    24541 aactcaccct caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc
    24601 ttctgttgtg ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta
    24661 caggctctgt ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa
    24721 acgctggcga atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg
    24781 catcgtcccc agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt
    24841 gccgacaagc cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac
    24901 actgcgcaaa cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt
    24961 attattggtc aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc
    25021 gacaatgcac atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg
    25081 caataccagc aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag
    25141 cctgccggta aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc
    25201 cgcgaacgtt tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtattgact
    25261 tacattaacc aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac
    25321 tgtggttaat tcgtcatggc gaaacacaag cgaatgtcga tggtctgtac agcggtcatg
    25381 cgcccacacc cctgaccgca cgcggtattg agcaagcgca aaatctgcat acactgctag
    25441 atgatgtttc ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc
    25501 gactggttct cagtgaccgc cagctccccg ttcatatcat ccctgaactc aacgaaatgt
    25561 tttttggcga ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact
    25621 atagcgcgtg gtgcaatgac tggcagcatg ctatccccac gaatggtgaa ggatttcagg
    25681 cattttcgca acgtgtggaa cgctttatcg caaggcttag tgaatatcag cactatcaga
    25741 atattttaat cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ttaattggca
    25801 tgcctgccga gtctatgtgg cattttcgcg ttgaccaggg gtgttggagc gccattgata
    25861 tcaaccaaaa attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaacg
    25921 catgactttt ctgttttttt acgggtaagc cgcaacgacc attgacagcc ccgggcaggc
    25981 tgatattctc cgcagccaga ctttttccgc cagacacgac tttgtagaaa ttgttttaca
    26041 aaaatggcga tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt
    26101 cggcgacaat tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct
    26161 atttgattca cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac
    26221 aaaattgatg acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc
    26281 atcaccgact gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct
    26341 gaccacgttg tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa
    26401 aacagcgccg actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa
    26461 gagagccgtc gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt
    26521 gtcgccgtgg gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt
    26581 cgttttccga aagatatgcc cttcgagctg attgaaattc cggccggcaa acgcggcaag
    26641 aatgcggaca tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc
    26701 aaaaaccgca ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc
    26761 gctgagctgg aacgctggaa gctggatggt cgcgacgtaa gtctactgat tggcgggcct
    26821 gaagggttgt cgcctgcctg taaagcggcg gctgagcaga gctggtcgct gtcggcgctt
    26881 accctccccc atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc
    26941 atcaccacca accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag
    27001 cggatgaaac tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc
    27061 cgggcgctgg tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg
    27121 tataatctgc aaattgttcg ctttaccgac taccagaccc gctctaatga aaaccgcatt
    27181 aagctggtgc ctatcgcgcc cagccgcggc attatctacg accgtaacgg tatccctctg
    27241 gccctcaacc gcactatcta ccagatagaa atgatgccgg agaaagtcga taacgtgcag
    27301 caaacgctgg acgctttgcg cagcgtggta gatctgaccg atgacgatat tgctgcattc
    27361 cgaaaagagc gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctaacc
    27421 gaagtacaag tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa
    27481 ggctataaac gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg
    27541 tcgaaaatca acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat
    27601 gcggcaacgc atgatattgg taagctgggc attgagcgtt actatgaaga cgtgctgcac
    27661 ggtcagaccg gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta
    27721 aaagaagtac caccgcaagc cggacacgat atttacctga cgctggatct caaactccag
    27781 caatatattg aaacgctgct ggcgggtagc cgcgcagctg tggttgttac cgaaccgcgt
    27841 acaggtgggg tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac
    27901 ggtatctcca gcaaagatta ttccgccttg ctgaacgacc cgaatacacc gctggtgaac
    27961 cgcgccacac agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg
    28021 gcattaagcg ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa
    28081 ctaccagggt cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat
    28141 gtcacaagat cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg
    28201 gggatcgatc gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc
    28261 gacctggcgg aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt
    28321 aaaaaaccgt ggtatcaggg tgacaccatt ccggttggta tcggccaggg ttactggaca
    28381 gcgaccccaa tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag
    28441 gttccgcatt tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacaaccg
    28501 catgaaccgc ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg
    28561 tacggtgttg ctaaccgccc taatggtacg gcgaataaat actttgccag cgcaccgtac
    28621 aaaattgcgg cgaaatctgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat
    28681 aatgcgcaca aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca
    28741 tacaacaatc cgcaagtggc tgtcgccatg attctggaga acggcggcgc gggtccggcg
    28801 gttggtacgc tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat
    28861 ctgcctgcgg aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc
    28921 cgaataaaaa aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgattttac
    28981 tggcattgct ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca
    29041 tgatggagcg taaaatcggc caaatcgcga tgggtctggt catcatggtg gtgatggcgc
    29101 aaattcctcc acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt
    29161 tgctggtggc ggtagatgct ttcggagcca tctctaaagg cgctcaacgc tggctggacc
    29221 tcggtattgt tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg
    29281 cgcgctttat caaccgcgac gtttgcccgc catcgttgaa gaatactggc atcgcgctgg
    29341 tgctgatatt tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc
    29401 tcgttgcgct ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg
    29461 gcgtcgcagt agtgctggta gcggcgttca ttccgattct gtggttcttc ctgatgcatg
    29521 attaccagcg ccagcgcgta atgatgctcc tggacccgga atcagaccca ctcggcgcgg
    29581 gctatcacat tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct
    29641 ggctgcacgg cactcagtca cagcttgaat ttctccccga acgccatact gactttatct
    29701 tcgcggtact ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca
    29761 ttctgctgat catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg
    29821 tcatggctgg tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg
    29881 taagcggtat tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg
    29941 cgctaattgt gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa
    30001 tgttgtcgaa aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc
    30061 gcggcaggaa tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta
    30121 ccgcagcctg cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc
    30181 gaaccactga acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc
    30241 gtgcaggatc cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaacca
    30301 ggcagtaacc tgacggcctc tggcgaagct ttcgatccga caaagctgac ggcggcccat
    30361 ccaacgcttc cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc
    30421 gtggtgcgca ttaatgatcg cggcccttac ggcaacgacc gcgttatttc gctttctcgc
    30481 gcggcagctg accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt
    30541 gtcgcccagg atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag
    30601 acttacgccc tgcctgcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct
    30661 ggcccgcagg gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc
    30721 ggcgcgccgg taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctagtgta
    30781 ctggaaggca gcgaaccgac gcctgcgcca cagcccgttg ttacagcttc gtcgacaacg
    30841 cctgcaacct cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac
    30901 tttatggtgc aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa
    30961 ctgggacaga agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc
    31021 cagcttggcc cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc
    31081 gaagcccaat tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt
    31141 gtcaatttct gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt
    31201 gcatttgcta tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg
    31261 aataccattt tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca
    31321 gcctttatct ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta
    31381 ccgcagatcg atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc
    31441 gaacagaacg cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac
    31501 gttatcggcc aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc
    31561 aacgacgcat gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa
    31621 ccgggcatgc aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac
    31681 gatgcttgtg tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg
    31741 atgaacagct acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt
    31801 ctggatgctg atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg
    31861 atccgtgacg taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt
    31921 attcgccagc tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc
    31981 aaaaccggac acactgacaa agcaggttac aatcttgttg cttctgcgac tgaaggccag
    32041 atgcgcttga tttctgcggt gatgggtgga cgtactttta aaggccgtga agccgaaagt
    32101 aaaaaactgc taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt
    32161 aaagagttcg cctctgaacc ggtttggttt agtgattctg atcgcgcttc gttaggggtt
    32221 gataaagacg tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat
    32281 gtgctgaaca gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc
    32341 aacttccagc ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc
    32401 ccggaaggta acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg
    32461 tttggttaaa aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt
    32521 aaaaaaactc ccgccttctg gcgggagttg ctatttaatt acgttacgcc ggagctgaca
    32581 tgaaaaccaa acttaacgaa ctgcttgaat tccctactcc ttttacttac aaagttatgg
    32641 ggcaggcgtt acctgagctg gttgatcagg tggttgaagt ggtacagcgc catgcgccag
    32701 gtgactacac cccaacggta aaaccaagca gcaaaggcaa ctaccactcg gtatctatca
    32761 ctatcaacgc cactcatatc gagcaggttg aaacactgta tgaagaactg ggcaaaatcg
    32821 atattgtccg catggttctg taactcgctt ctccgttacc cggctcctgg tcgggtaact
    32881 cccctctcct cgctgtgata tacttttccc ccacttttac tcattctcca cggagatgcc
    32941 gttttgtatc aggataaaat tcttgtccgc cagctcggtc ttcagcctta cgagccaatc
    33001 tcccgggcta tgcatgaatt caccgatacc cgcgataata gtacccttga tgaaatctgg
    33061 ctggtcgagc actatccggt attcacccaa ggtcaggcag gaaaagcgga gcacatttta
    33121 atgccgggtg atattccggt gatccagagc gatcgcggtg ggcaggtgac ttatcacggg
    33181 ccgggacaac aggtgatgta tgtgttgctt aacctgaaac gccgtaaact cggtgtgcgt
    33241 gaactggtga ccttgcttga gcaaacagtg gtgaataccc tggctgaact gggtatagaa
    33301 gcgcatcctc gggctgacgc gcctggtgtc tatgtcgggg aaaagaaaat ttgctcactg
    33361 ggtttacgaa ttcgacgcgg ttgttcattc cacggtctgg cattaaacgt caatatggat
    33421 ctttcaccat ttttacgtat taatccttgt gggtatgccg gaatggaaat ggctaaaata
    33481 tcacaatgga aacccgaagc gacgactaat aatattgctc cacgtttact ggaaaatatt
    33541 ttagcgctac taaacaatcc ggacttcgaa tatattaccg cttaattcca tacatcaatg
    33601 gcccaattta cactgggtca ttactcataa tacctgctaa tgctttacat ctccttcgcg
    33661 tttgtatatt ttctttatca cttgcagaaa tgcggtgcca cgccttgctc cgtaactcac
    33721 cccattgatt aacctattga aatagttgat tatggtgagc aaaagcgttc cgttccaatt
    33781 agacataaga ttattgtaat tttttaaata ttattttccc atgaataaaa ttggagtaag
    33841 tgcgtggata gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa
    33901 ccacaaatat ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct
    33961 gtatatgtac ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca
    34021 atcagtcagt ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa
    34081 ggccagggtg tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc
    34141 cttgagtcca ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata
    34201 actattgcga ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt
    34261 aaaactcact atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa
    34321 ctcagtcagt ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg
    34381 caacatcatg ttctgttcac cgacaatatg gtgttaattt gccgcaaggg aaatccacta
    34441 ctctctttag aagatgacag agagactatc gacaacgctg cgcatgtact tctgttaccg
    34501 gaagggcaaa atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa
    34561 atcaatttca ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg
    34621 ttagcgatta ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa
    34681 ctgccttttc cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc
    34741 atcctgcgtg atcccatcct gcacggagta atagatgtta tccgtaatgc attttgaaaa
    34801 agtattgtta cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt
    34861 ttacattttc gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc
    34921 gttaattcaa aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt
    34981 aattcgcaac tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa
    35041 ataccgcgat gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga
    35101 agccctgctg cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat
    35161 ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc
    35221 ctgccctaac ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc
    35281 tatttgtacc cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga
    35341 tgccaatgaa ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt
    35401 tatcacctcc gttgaccgtg atgacctgcg cgatggcggt gcccaacact ttgcggattg
    35461 tattactgcc attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt
    35521 ccgcggtcga atggatcgtg ctctggatat tctgactgca acgcccccag atgtgttcaa
    35581 ccataacctg gaaaacgtac cgcgtattta ccggcaggta cggcctggcg cagattacaa
    35641 ctggtcgctg aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc
    35701 tggtctgatg gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct
    35761 gcgccgtcat ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca
    35821 cctgccggtt caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct
    35881 ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga
    35941 tttgcaggcg aaaggaatgg aagttaagta atcctgtaat aattacttac atctgtctga
    36001 taaaaaaccc gctttttgaa gcgggttttt tgtatcgaac aagatattga gggagcgtcc
    36061 tgctcgccac gtcactcttt atgagagagc ttttcagcct gaagatcaac gtctgcgcct
    36121 tttttcgccg cagcatcgtc atcattcatc gccttcttga accctttaat ggccgctcca
    36181 aggtctccgc ccagcgtacg taacttctta gtcccaaaca gcagaacgac cagcgccgca
    36241 actaccagca gtttggtaat actaatctca cccatagata ccttcttgac atataaccgg
    36301 caacagaacg cgccttaaaa accagagaat attaacggtc attcgacgcg cgcacacaat
    36361 agcaatctgt aacaaggtga atcaagcgcc gagtaaaaaa acatcataat aattgcggcg
    36421 gcgcaaagcg acggttgttt aagatgggca gttgcgcgcg cacctgacgc acacgttcgg
    36481 gcgtcacttc cgccataatg agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa
    36541 agggatctat aatccggctt tgcccgatat ttttgttccc gcactccccc gccgccacca
    36601 tataacaggt ggtatccagc gcacgagcgg caagcaacgt tgaccagtga tgctctttga
    36661 gcggcccacg aacccaggcg gcaggaagta ccaggatttc agctccctgt aaagcctgtg
    36721 ccagcgccag ctctggaaag cgtaagtcat aacaggtcat cagaccgacc ttcatccctt
    36781 ccacctccag taacggagct atttcattac cagcatcaac atggcgtgat tcctgaatgg
    36841 caaatgcatc atagagatgc agtttggcat aacgggcgac gatgtttcct gcctgaagtg
    36901 ccaccagcat attccatgcg cgccccggcg ttgaaggaag atgaatcgtc agaattgtcg
    36961 tcatcatgtt acgtttactt tctcgccgta aacgtccgag gaattcgcct tccagcagct
    37021 gtgctgattt aaccgataga tctgcatcat gatcatcgcg cgccagcaat gcttccggca
    37081 ggacaaacag cgatacgtcg ttttccgccg cctgcgccat caacgaggca caaatctcag
    37141 cgttcttttc ccacacagat gtaacagcaa actgtcctgc agcaaccagc attgccacct
    37201 cctgtcagag tcgtaaaatt cgccaacgac gattattcgg cgttacactt atcactcata
    37261 caaatcaaat agcaggattt tgcagtgtta caacttcttt tagcagtttt tattggcggt
    37321 ggtacgggaa gcgtagcgag atggctgtta agtatgcgat ttaacccgct gcatcaggcg
    37381 attccgttgg ggacgctggc agcaaacctg attggggcat tcatcatagg aatgggattc
    37441 gcctggttca gcaggatgac gaacattgat ccagtgtgga aagtattaat caccaccgga
    37501 ttttgtggcg gtctaacaac cttctcaaca ttttcggcag aagtggtgtt tttgttacaa
    37561 gagggccgct ttggctgggc attactgaac gttttcgtca accttctggg gtcttttgcc
    37621 atgaccgcac tggcattctg gctgttttcg gcctcaaccg tacactaaag accaaaaaaa
    37681 acccgctgat taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt
    37741 gcagcagaag ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca
    37801 agagttttaa aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg
    37861 tcttccggag taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta
    37921 atcttagaca tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttatc
    37981 agtacaccaa ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg
    38041 cgttattttt gatgttgctt attttagtta ttgattttaa agaagttact aaaacttcat
    38101 ttgtctcaaa actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc
    38161 ggaatgtagg tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga
    38221 accgggagag ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga
    38281 cctaaatgaa aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca
    38341 atcggttccc atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt
    38401 cctttttcat cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga
    38461 tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa
    38521 tcataatgtt caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatcctc
    38581 tcatctgcgt tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag
    38641 atagcgacat atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta
    38701 ctagcatagc aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg
    38761 aatagctgct ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag
    38821 atgatgcgtc gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt cttttttgat
    38881 gacaaaatgc aatcaaggaa aaattatttt atttttttaa cgagatacaa caatcatctt
    38941 aacgaagtat ataatatata tcgtcaacca tgctcatatt agattatttt ttgagcgcac
    39001 agccgcagct aacaccattg caattaacaa atttgcatca atccaccatc aatttacaca
    39061 cattattatg tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac
    39121 agcaacttca atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt
    39181 tattgggcat attttttcct taagctttag gaagttttta ttatttactt tgggcctgga
    39241 gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg
    39301 tgtggctcgc tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct
    39361 gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc
    39421 aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg
    39481 cggcgacctc ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat
    39541 cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc
    39601 ttacctgctg atgattgccg cctattttgt tgcttgtctg atgtcactgg ccgtctcttc
    39661 cgcaaccggt ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg
    39721 tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg gcggcgatta ttctcgcacc
    39781 gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc
    39841 cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt
    39901 cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag
    39961 tgaaatcacc accactgccc ccgcgtttta tgccattttg ccgttcacgc cgatcatcgg
    40021 tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat
    40081 ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt
    40141 ctctggtctg gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct
    40201 gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct
    40261 gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat
    40321 tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt
    40381 tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc
    40441 gatgctgcag gcgtcaaacc tggatcgtac cctatcaccc gtttctggcg tagtcgttgc
    40501 ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct cggtgccggt
    40561 gcttgttggg ctagtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc
    40621 agcggtcaca gacaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt
    40681 cacccactgt ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata
    40741 atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg gctgatggtc
    40801 agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc
    40861 accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga
    40921 gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc
    40981 atatgtttac gctggcggaa acgggttagc gtttgcccca gtgtattgat tcacttgaag
    41041 tacgaaaaaa ccgggagaac attggattat tcgggatctg atgggattag atttggtggg
    41101 gcttgcaagc ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa agatagtaaa
    41161 aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac actataaatc atttcaatca
    41221 tagcttcaaa aaacgttcaa aaccgataac aaccaggctg tcaccaaatg actcatatca
    41281 caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt
    41341 ttagctggct catggttgtt atacggcttt tttcgcatct tgtggttcca acaaaggagc
    41401 tacaggctct tcctgtttat gaacaccata cattgacaaa atctcttggc gagtttctgg
    41461 aggtaattta tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac ccagcgttgc
    41521 caatagggtg tcaacggaga tggaggggcg ttcttgcatt aatccggaaa gcccatattt
    41581 agccatgttt agctctatct ctcggacttc attttctcct agtttattga ttaacaggga
    41641 gaacattttc aatgtatcag cattcatttc ttcgatagca attttttgca tagccggtat
    41701 tgccgggata tattcaaccc caggcccgcg acgcccttct tgtgaacgct tcggccatcc
    41761 ttctttcttt gctcgtttat gcaccccctg tattgtccca ggcattccat ccagttcagt
    41821 aatttgtttg ggtgtcatcc actggtttat ctcatttgcg cttttcataa actaagttta
    41881 ccagatttat ctatataaaa atcaatggat tacaaaataa accacattaa ctgatataaa
    41941 ccggttttgt ttatttggta atgtataaaa cagataatca agacgaatta tttgttttgg
    42001 taacccttta tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga ttggcatcgg
    42061 gccgacgtga ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt gtccagacgt
    42121 tcaggttatt caaccgggac gcttgcaaat gcattagtca ggccatggcc taaaggtgaa
    42181 cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag tagatatcta
    42241 agggatcaca atgagagagt ggatatcagc aattgaatgc atgggtttat ctggtttacc
    42301 tggttcatta atgggggttc acaaaagagc tcagagagaa ggatggctca agagaagaag
    42361 agaaggaatt agaggggttg cttacgaata ccatatatct tctcttccta acattgctcg
    42421 agctgaagtt ttgctccaac aaggaaaatt taaaaccagt cagggatgtt ttgaaatcgc
    42481 ccgccccacg ctggaagctc atgattatga tcgtgaggca ctatggagca aatgggataa
    42541 agccagcgat tcccagcgca gcctggctga aaaatggttg ccctcaattc aggccacaga
    42601 cgaaatgctg aaccagggga tttcaacgaa aacggctttt gcgaccgttg cagggcatta
    42661 ccaggtcagc gcatccactt tgcgggacaa gtattaccag gtacagaagt ttgcgaagcc
    42721 tgactgggcg gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg ttcacaaaag
    42781 tgaatttgac gaggatgcct ggcagtttct gattgcagat tatctgcgac cggaaaaacc
    42841 cgccttccgc aaatgttatg agcgtctgga actggcagcc cgcgagcatg gctggagtat
    42901 tccctcccgt gccacggcct ttcgccggat tcagcaactg gacgaggcaa tggttgttgc
    42961 ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg gcacagcagc gaactgtgga
    43021 acacctggac gccatgcagt ggatcaacgg cgacggttat ctgcataacg tctttgtacg
    43081 ctggtttaac ggtgatgtga tccgtccgaa aacatggttc tggcaggatg tgaaaacccg
    43141 aaaaattctg ggctggcgct gcgatgtgag cgagaacatt gattcaattc gcctctcgtt
    43201 catggatgtt gtgactcgct acagtatccc ggaggatttt cacatcacca ttgataacac
    43261 ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc aatcgctacc gctttaaggt
    43321 aaaagaggac gatccaaaag gactgttttt actgatgggt gcgaaaatgc actggacaag
    43381 cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt tcggtgttgg
    43441 tgggcttgag gaatacgttg ataagcatcc ggcactggct ggcgcatata cggggccaaa
    43501 tccgcaggca aaacctgata actatggcga ccacgctgtt gatgcagagc tgtttctgaa
    43561 aacctttgcc gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa cagaaatgtg
    43621 cgggggcaaa ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa cgattgtgcg
    43681 taagccaacc gaagaacaaa aacggatgct gttactgcct gccgaggcgg tgaacgtttc
    43741 acgcaaaggc gagtttacgc ttaaagttgg cggctccctt aaaggcgcga aaaacgttta
    43801 ttacaacatg gcattaatga atgccggagt gaaaaaagtt gtggtcagat ttgatccaca
    43861 gcagttacac agcacggttt attgag
//
LOCUS       DAJTUN010000019        43643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTUN010000019 DAJTUN010000000
VERSION     DAJTUN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.019"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            390..1520
                     /gene="fruB"
                     /locus_tag="OO470_001005"
     CDS             390..1520
                     /gene="fruB"
                     /locus_tag="OO470_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6250334.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            1520..2458
                     /gene="fruK"
                     /locus_tag="OO470_001006"
     CDS             1520..2458
                     /gene="fruK"
                     /locus_tag="OO470_001006"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6250335.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            2475..4166
                     /gene="fruA"
                     /locus_tag="OO470_001007"
     CDS             2475..4166
                     /gene="fruA"
                     /locus_tag="OO470_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6250336.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            4590..5531
                     /locus_tag="OO470_001008"
     CDS             4590..5531
                     /locus_tag="OO470_001008"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6250337.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            5519..6457
                     /gene="psuG"
                     /locus_tag="OO470_001009"
     CDS             5519..6457
                     /gene="psuG"
                     /locus_tag="OO470_001009"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6250338.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            6551..7801
                     /locus_tag="OO470_001010"
     CDS             6551..7801
                     /locus_tag="OO470_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6250339.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7872..8566)
                     /gene="yeiL"
                     /locus_tag="OO470_001011"
                     /pseudo
     CDS             complement(7872..8566)
                     /gene="yeiL"
                     /locus_tag="OO470_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            8697..9638
                     /gene="rihB"
                     /locus_tag="OO470_001012"
                     /pseudo
     CDS             8697..9638
                     /gene="rihB"
                     /locus_tag="OO470_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            9738..10988
                     /locus_tag="OO470_001013"
     CDS             9738..10988
                     /locus_tag="OO470_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6250340.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11044..12132)
                     /locus_tag="OO470_001014"
     CDS             complement(11044..12132)
                     /locus_tag="OO470_001014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6250341.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(12135..12992)
                     /gene="nfo"
                     /locus_tag="OO470_001015"
     CDS             complement(12135..12992)
                     /gene="nfo"
                     /locus_tag="OO470_001015"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6250342.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(13066..14115)
                     /locus_tag="OO470_001016"
     CDS             complement(13066..14115)
                     /locus_tag="OO470_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6250343.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            14214..15095
                     /locus_tag="OO470_001017"
     CDS             14214..15095
                     /locus_tag="OO470_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6250344.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15301..16770
                     /gene="lysP"
                     /locus_tag="OO470_001018"
     CDS             15301..16770
                     /gene="lysP"
                     /locus_tag="OO470_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6250345.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            17062..19053
                     /gene="cirA"
                     /locus_tag="OO470_001019"
     CDS             17062..19053
                     /gene="cirA"
                     /locus_tag="OO470_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6250346.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(19085..19920)
                     /gene="yeiG"
                     /locus_tag="OO470_001020"
                     /pseudo
     CDS             complement(19085..19920)
                     /gene="yeiG"
                     /locus_tag="OO470_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            20178..20846
                     /gene="folE"
                     /locus_tag="OO470_001021"
     CDS             20178..20846
                     /gene="folE"
                     /locus_tag="OO470_001021"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6250347.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20863..21939
                     /locus_tag="OO470_001022"
     CDS             20863..21939
                     /locus_tag="OO470_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6250348.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            22081..23121
                     /gene="galS"
                     /locus_tag="OO470_001023"
     CDS             22081..23121
                     /gene="galS"
                     /locus_tag="OO470_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6250349.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            23401..24399
                     /gene="mglB"
                     /locus_tag="OO470_001024"
     CDS             23401..24399
                     /gene="mglB"
                     /locus_tag="OO470_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6250350.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            24460..25980
                     /gene="mglA"
                     /locus_tag="OO470_001025"
     CDS             24460..25980
                     /gene="mglA"
                     /locus_tag="OO470_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6250351.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            25996..27006
                     /gene="mglC"
                     /locus_tag="OO470_001026"
     CDS             25996..27006
                     /gene="mglC"
                     /locus_tag="OO470_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6250352.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(27042..28255)
                     /gene="preA"
                     /locus_tag="OO470_001027"
                     /pseudo
     CDS             complement(27042..28255)
                     /gene="preA"
                     /locus_tag="OO470_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(28249..29487)
                     /gene="preT"
                     /locus_tag="OO470_001028"
     CDS             complement(28249..29487)
                     /gene="preT"
                     /locus_tag="OO470_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6250353.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(29681..29920)
                     /locus_tag="OO470_001029"
     CDS             complement(29681..29920)
                     /locus_tag="OO470_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6250354.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(29923..30642)
                     /gene="sanA"
                     /locus_tag="OO470_001030"
     CDS             complement(29923..30642)
                     /gene="sanA"
                     /locus_tag="OO470_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6250355.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(30792..31676)
                     /gene="cdd"
                     /locus_tag="OO470_001031"
     CDS             complement(30792..31676)
                     /gene="cdd"
                     /locus_tag="OO470_001031"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6250356.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(31806..32501)
                     /locus_tag="OO470_001032"
     CDS             complement(31806..32501)
                     /locus_tag="OO470_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6250357.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(32498..32896)
                     /locus_tag="OO470_001033"
     CDS             complement(32498..32896)
                     /locus_tag="OO470_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6250358.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            33135..34082
                     /gene="dusC"
                     /locus_tag="OO470_001034"
     CDS             33135..34082
                     /gene="dusC"
                     /locus_tag="OO470_001034"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6250359.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(34453..34536)
                     /gene="yohP"
                     /locus_tag="OO470_001035"
     CDS             complement(34453..34536)
                     /gene="yohP"
                     /locus_tag="OO470_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6250360.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            34760..36196
                     /gene="mdtQ"
                     /locus_tag="OO470_001036"
     CDS             34760..36196
                     /gene="mdtQ"
                     /locus_tag="OO470_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6250361.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            36249..37010
                     /locus_tag="OO470_001037"
                     /pseudo
     CDS             36249..37010
                     /locus_tag="OO470_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(37127..37705)
                     /locus_tag="OO470_001038"
     CDS             complement(37127..37705)
                     /locus_tag="OO470_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6250362.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            37875..38462
                     /locus_tag="OO470_001039"
     CDS             37875..38462
                     /locus_tag="OO470_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6250363.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            38636..39568
                     /gene="pbpG"
                     /locus_tag="OO470_001040"
     CDS             38636..39568
                     /gene="pbpG"
                     /locus_tag="OO470_001040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6250364.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(39606..41321)
                     /gene="dld"
                     /locus_tag="OO470_001041"
     CDS             complement(39606..41321)
                     /gene="dld"
                     /locus_tag="OO470_001041"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6250365.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            41517..>43643
                     /gene="bglX"
                     /locus_tag="OO470_001042"
     CDS             41517..>43643
                     /gene="bglX"
                     /locus_tag="OO470_001042"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6250366.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFE"
BASE COUNT        10634 a        10689 c        11612 g        10708 t
ORIGIN      
        1 agactgcggg ggagttatgc atgctggtcg atcactggaa gaggaagacg cggagtttac
       61 gctgcttgtc agaaaataaa cagacagcga aatcaaaagc gccacgaata atcaaaatgc
      121 catttcactg ttgctgattg gctgctgacg ctgctgaaat gaaattgctg ataacggatt
      181 ttcccatcag caattaggaa aaatgacaaa aaattgtgca gcacatcaaa cttttgctca
      241 taactttacg gctttccttg cgtgctgaaa cagaagtatt atgctttctt gaaacgtttc
      301 agcgcgatct tgtctttaac cctaagctag gttggcgctt tttttctcat agaggctgaa
      361 tcgtttcaat tcagcaagag aggagaacta tgttccagtt atccgtacag gacatccatc
      421 cgggcgaaaa ggccggagac aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc
      481 aggccggtaa tgtagcagaa ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct
      541 caacgttcct cggcaatggt attgctattc cacacggcac taccgacacc cgcgatcagg
      601 tgctgaaaac cggcgttcag gtatttcagt tcccggaagg cgtcacctgg ggtgacggtc
      661 aggtagcgta cgtggcaatc ggtattgctg ccagctcgga taaacatctg ggcctgctac
      721 gccagctgac ccacgtactg agcgatgatt ccgttgctga acaactgaag tcagcaacaa
      781 cagcagaaga acttcgcgca ttactgatgg gcgaaaagca gagtgagcag ctgaagctcg
      841 acaacgaaat gctgacgctg gatatcgtcg ccagcgatct gctgactctt caggcgctga
      901 acgctgcgcg tctgaaagag gcgagggcag ttgacgccac tttcgtcacc aaagccatca
      961 atgaacaacc gctgaatctc ggacagggta tctggctgag cgatagcgcc gaaggcaatc
     1021 tgcgtagtgc gattgcggta agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg
     1081 cagccatgct ggtgagtgtg gcgatgaatg acgatcagcc cattgcggtt cttaagcgtc
     1141 tcgctgattt gttgctcgac aataaagctg accgcttgct gaaagcggat gcggcaacgt
     1201 tgctggcgct gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg
     1261 tagtgcgcaa tgaacacggc ctgcatgctc gtccaggtac catgctggtc aataccatta
     1321 aacaatttaa cagtgatatt accgtgacaa accttgatgg caccggcaaa ccggcaaacg
     1381 gacgtagtct gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta
     1441 ccgcccaggg tgcagatgct gaacaggcgc tgaaagccat tggcgacgct atcgctgctg
     1501 gtcttgggga gggcgcataa tgagcagacg tgttgctacg atcaccctta atccggctta
     1561 tgaccttgtt ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac
     1621 cggtctgcat gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag acctgggaat
     1681 tgatgtcacc gttggcggct tcctgggtaa agacaatcag gatggttttc agcaactgtt
     1741 cagcgagctg ggcattgcca accgtttcca ggttgtacag gggcgcaccc gaattaacgt
     1801 taagctgacg gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt tcgaagtcac
     1861 ccccgccgac tgggaacgct ttgtgactga ttctctgagc tggctcggtc agttcgatat
     1921 ggtctgtgtc agcggaagct taccgtcagg cgtcagcccg gaagcgttca ccgactggat
     1981 gactcgcctg cgtagtcagt gtccttgcat tatctttgat agtagccgtg aagcgttagt
     2041 agcaggtttg aaagcggcac cgtggctggt gaaacctaac cgccgcgagt tggaaatctg
     2101 ggcaggccgt aaactgcctg aaatgaaaga tgtgattgaa gctgcacatg cgctacgtga
     2161 acaaggtatt gcgcatgttg ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc
     2221 ctccggcgaa tggatcgcta aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg
     2281 ggattctatg gttggtggcc tgatttatgg cttgctgatg cgtgaatcca gtgaacacac
     2341 actgcgtctg gcgacagctg ttgcagccct ggcggtaagt caaagcaatg tgggtattac
     2401 cgatcgtccg cagttggccg caatgatggc gcgcgtcgac ttacaacctt ttaactgaca
     2461 gcaggagagg cataatgaaa acgctgctga ttattgacgc taatctcggt caggcacgcg
     2521 cctatatggc gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg
     2581 acaatccgaa cgacgcagaa atggcgattg ttctcggtga ttccatcccg aatgacagcg
     2641 cgctgaacgg taaaaatgtc tggctgggtg atatttcccg ggcagttgcg caccctgagc
     2701 tgttcctgag tgaagccaaa ggccatgcga aaccttacac tgcgccggtc actgcgacag
     2761 caccggttgc cgccagcggt ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg
     2821 tagcacacac ctttatggcg gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt
     2881 gggtgaaagt tgaaacccgt ggttctgttg gcgcgggtaa tgcaatcact cccgaggaag
     2941 tagccgcagc ggatctggtg attgtggcgg cagatatcga agtggatctg gcgaaatttg
     3001 ctggtaaacc gatgtatcgt acctctaccg gtctggcgct gaagaaaacc tcgcaggaac
     3061 tggataaagc ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct caaacggcga
     3121 ccactgaagg taagaaagag agtgcaggcg cttatcgtca cttgctaacg ggcgtctctt
     3181 acatgctgcc gatggtcgtt gctggtggtc tgtgtatcgc gctttctttt gcttttggta
     3241 tcgaagcgtt taaagagccg ggtacgttgg cagcggcgct gatgcagatt ggtggtggtt
     3301 cagcctttgc gctgatggtg ccggtactgg caggttatat tgccttctcc attgccgatc
     3361 gtccgggcct cacgccgggt ctgattggcg gtatgctggc ggtcagcacc ggttctggct
     3421 tcattggcgg tattattgcg ggcttcctgg ctggttacat tgcgaagtta atcagtacgc
     3481 aactgaaact gccgcagagt atggaggcgc tgaaaccgat cctgatcatt ccgctaattt
     3541 ccagtctggt ggtcggtctg gcgatgatct acctgatcgg taaaccagtt gctggcattc
     3601 tcgaagggct gactcactgg ctgcagacca tggggactgc gaatgcggtt ctgctggggg
     3661 cgatcctcgg tggcatgatg tgtactgaca tgggcggtcc ggtaaacaaa gcagcgtacg
     3721 cattcggtgt gggtctgctg agtactcaaa cctatggccc gatggcggcg attatggcgg
     3781 caggtatggt gccaccgctg gcaatgggcc tggcaacaat ggtggcgcgt cgcaaattcg
     3841 acaaagcgca gcaggaaggt ggcaaagccg ctctggtatt gggactgtgc ttcatttcgg
     3901 aaggtgcaat tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgc tgtatcgtgg
     3961 gtggggcgct gactggcgca atctcaatgg cgattggtgc gaaactgatg gcaccgcacg
     4021 gtggtctgtt tgttctgctg atccctggcg ctattacgcc ggtattgggt tacctggtag
     4081 caattattgc cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa cgtccggaag
     4141 tggacgcagt agcgaaagca gcgtaataaa aggtgtgtta cagggcagaa atttcctgcc
     4201 ctgtaacatt tgatgagcgc aaagagaatg accagcttgt gaatgtaatt ttatgaattt
     4261 aaatggtttt atctttcagt agaaacttca aacgttcatc aaacactaag gttatttcct
     4321 caagggcaga ttattttctg ccctttttta gtgttcctgt tctgtgcatc ccgtcacaaa
     4381 ttcctcgctt ttgtaattca ggcatttaat cgcattttgt gagcctgctc aaatctgccc
     4441 attttgcctc tctggcggta atttacttta cgtaaaacat gcatactatg agcacatgtt
     4501 taaatatgaa cacatgttta aaccgtcttt gcagtatcta tattcttgtg gattgacagg
     4561 gttggtaatt ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag
     4621 gttcggcgaa tattgatgtc gccggatatt cacatgaatc attaaattat gcggattcaa
     4681 atccaggtaa aataaaattt acgcctggtg gagtagggcg caatattgca caaaacctgg
     4741 cgttgctggg taacaaagcc tggctactga gcgccgtagg cagtgatttt tatggtcaat
     4801 cgctgctaac gcaaaccaat caatctggtg tttatgtcga taaatgcctg attgtgccgg
     4861 gagaaaatac gtcgagttat ttatcattac tcgataatac cggtgaaatg ctggttgcta
     4921 taaatgacat gaatattagc aacgctatta cagctgaata tctcgcacag caccgtgaat
     4981 ttattcagag ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag gcactggcat
     5041 ggattctgga taatgccgcc aacgtacccg tatttgtcga tccggtttca gcgcggaaat
     5101 gtgtcaaagt gcgcgaccgt ctaaatcaga tccacactct gaagccaaac cgccttgaag
     5161 cggaaaccct gagtgggatt gcgctgtcag ggcgtgacga tgtggcaaaa gttgctgcct
     5221 ggttccatca acatggcctg aaccgactgg tattgagcat gggcggcgac ggcgtttatt
     5281 acagcgatat cagcggtgaa aatggctggt ctgcgccgat caaaaccaat gttattaatg
     5341 ttaccggagc gggcgatgcc atgatggcgg gacttgcttc gtgttgggta gacggaatgc
     5401 cgtttgccga atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat
     5461 acaccaataa ccccgattta tcgattgcca acgttatatc gttagtggag aacacagaat
     5521 gtctgaatta aaaatctccc ctgaattatt acaaatttct ccggaagtgc aggacgcttt
     5581 aaaaaacaaa aaaccggttg tggcgctgga atcgaccatt atttctcacg ggatgccgtt
     5641 cccacaaaat gcccagaccg caattgaagt agaagaaact attcgtaaac agggcgcagt
     5701 acctgccact atcgccatta ttggcggcgt gatgaaagtg ggtttaagca aagaaaaaat
     5761 tgaattactg ggtcgtgaag ggcataacgt gaccaaagtt agtcgtcgcg atttaccttt
     5821 tgttgttgcc gccggaaaaa atggcgcaac cactgtggct tcaacgatga ttattgcggc
     5881 gcttgccgga attaaagtat ttgccaccgg gggaattggt ggtgtgcatc gcggggcgga
     5941 acataccttc gatatttctg ccgatttgca agaactggca aatactaatg tcaccgttgt
     6001 ttgtgccggg gcgaaatcta ttctcgattt aggattaacc actgagtatt tagaaacctt
     6061 cggtgtgccg ttaattggct atcagactaa agcgctgcct gcgtttttct gccgtaccag
     6121 ctcgtttgac gtcagcattc gtctcgacag cgccagtgaa attgcccgtg caatggcggt
     6181 gaaatggcaa agcgggctga acggtggcct cgtggtagcg aacccgatcc cggaacagtt
     6241 tgcgatgccg gaggaatcta tcaatgcagc catagatcaa gccgtcgccg aagccgaaga
     6301 gcagggcgtt attggtaaag aaagtacacc gttcctgctg gcgcgcgttg ctgagctgac
     6361 cggcggtgac agcctgaaat ccaatatcca gctggtgttc aacaacgcca ttctggcgag
     6421 tgaaattgcc aaagaatacc agcgtctcgc gggttaattt ctgacgggca gaaagccgtc
     6481 gcccgtactc atcaagcata ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg
     6541 gaaaatcatt atggatataa tgagaagtgt tgtggggatg gtggtgttac tggcaatagc
     6601 atttctgttg tcagtgaata aaaagagcat cagtttgcgc acggttggag ccgcactgct
     6661 gctgcaaatc gctattggtg gcatcatgct ctacttccca ccgggaaaat gggcagtaga
     6721 acaggcggca ttaggcgttc ataaagtgat gtcttacagt gatgccggta gcgccttcat
     6781 ttttggttcg ctggttgggc cgaaaatgga tgtcctgttt gacggtgcgg gttttatctt
     6841 cgcctttcgc gtacttccgg cgattatttt cgttactgcg ctcatcagtc tgctgtacta
     6901 cattggcgtg atggggctgc tgattcgcat ccttggcagc attttccaga aagccctcaa
     6961 catcagcaaa atcgaatctt ttgttgcggt taccactatt ttcctcgggc aaaatgagat
     7021 cccggcgatc gttaaaccgt ttatcgatcg cgtgaatcgc aacgagttgt ttaccgcaat
     7081 ttgtagcggg atggcgtcca ttgctggttc ggtgatgatt ggttatgccg gaatgggcgt
     7141 accaattgac tacctgttag cggcatcgct gatggcgatc cccggcggta ttttgtttgc
     7201 acgtattctt agcccggcaa ccgagccttc gcaggtcaca tttgaaaatc tgtcgttcag
     7261 cgaaacgccg ccaaaaagct ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa
     7321 aatcgccgct ggtgtagcga cggtggtaat ggcgtttgtc gcaattattg cgctgatcaa
     7381 cggcattatc ggcggaattg gcggctggtt tggtttcgcc aatgcctctc tggaaagtat
     7441 ttttggctat gtgctggcac cgctggcgtg gatcatgggt gtggactgga gtgatgccaa
     7501 ccttgctggt agcctgattg ggcagaaact ggcgattaac gaattcgtcg cttacctgag
     7561 tttctcccca tacctgcaaa cgggcggcac gctggaagtg aaaaccgtgg cgattatatc
     7621 ctttgcgctt tgtggttttg ctaactttgg ttctatcggt gttgtcgttg gcgcattttc
     7681 ggctatttcg ccaaaacgtg ctccagaaat cgcccagctt ggtttacggg cgctggcagc
     7741 agcaacgctt tccaacctga tgagtgcgac tattgccggg ttctttattg gtctggcgta
     7801 atgctcgatt tgtagaccgg ataaaacgta ttaagcgttg catccggcaa tcgcgctatt
     7861 gcaaataatt tttactgcat catcccggag aatttattct ccgggtccat ctccagcgcc
     7921 agtcctgaca actgctttct gtttttaatt agatatcctt tcttttaatt aataaaccgt
     7981 cgtgaatgaa ttgcgcgaga acatataaaa gatgtcgata agaaacgcct aaatactctg
     8041 ccgcttgcgt atgcttttcg tgataaagat caccttcttg tgagagtaat ataaatgctg
     8101 ccaggcgatt cactaacgga aatgattgat tctgagttaa agaaacaata ttacgataat
     8161 ttttatgact taaggtgacg caaagttttc gtaaaaatag ggtgtcgttt aataacagcg
     8221 gacggtaatg tttcataggg agcgcaaggc accaacactc ttcaatagcc tgcaccgcac
     8281 gcggttcatg gtctttgtcg attaactcaa tctcgccaat aaaacaggga gcggcaaaga
     8341 aatcgatcag cgacacgcga ccattggcaa gcgtggcgta aagcctggcg cggcctcgcg
     8401 tcagataaaa cagccatgac ggctgttgcc cttcctgcac aatgtagtca cgcgctaaaa
     8461 aatgaaacag ccgcgtatcg gctgaaacat ccgttgaaaa gcaatcctta aacgcagatt
     8521 cactcatcaa ttgctgctta aggtcattat tgtggatttc tttcacgatg tttatccgct
     8581 atgagatttc tcatatttag catacgtacg ctgttgttaa gattcaatca ttggcccggt
     8641 cgaattttct ttgctgtaga aaaaaataac gcaactggaa acagaggaaa taaacaatgg
     8701 aaaagagaaa aattattctg gattgtgagc cgggtcatga tgatgctatt gcaatgatga
     8761 tggcagcgaa acatccggca atagatttat taggcatcac tattgtagcg ggtaatcaga
     8821 cgcttgataa aacattaatt aatggcctga atgtttgcca gaaactggag attaatgttc
     8881 cggtttatgc ggggatgccg caacccatta tgcgtaaaca aatcgttgcc gataatattc
     8941 acggtgaaac cggactggat ggcccggtat tcgagccgct gacccgctag gcagaaagta
     9001 cccatgcggt gaaatatatc atcgataccc tgatggcaag cgatggcgat atcactctgg
     9061 tgccggttgg tccgctttca aatatcgcgg tggcaatgcg tatgcaaccc gcaatcctgc
     9121 ccaaaatccg tgaaattgtg ctaatgggcg gcgcttacgg tacaggcaac ttcaccccat
     9181 ctgccgagtt caatatcttt gccgacccgg aagccgcccg cgtggtgttc acctccggcg
     9241 ttccattagt gatgatgggc ctcgatctta ccaaccagac cgtttgcacc ccggatgtaa
     9301 ttgctcggat ggaaagggta ggcggccctg ccggagagct gttcagcgac atcatgaact
     9361 tcactctcaa aacgcagttc gaatactacg gcctggctgg cggcccggtg catgacgcca
     9421 cctgcattgg ttatctgatc aaccctgatg gcattaaaac ccaggatatg tacgtcgaag
     9481 tggacgtcaa cagcggccct tgctacgggc gtacggtctg cgacgaactg ggcgttcttg
     9541 gcaagcccgc taataccaaa gtcggcatca ctattgatac cgactggttc tggggcttag
     9601 tcgaagagtg cgtgcgcggc tacatcaaaa cccattaagt gttgataaca agccgggaca
     9661 gtatcgcaag atgcaggccc ggctatttct gatgggcaga aagccgtcgc ccaaaatgac
     9721 atgaagagat aatcactatg gatgtcatga gaagtgttct gggaatggtg gtactgctgg
     9781 caattgcgtt tttactgtca gtaaacaaga agaagatcag cctgcgtacc gttggcgcgg
     9841 cgttagtgtt acaggtcgtg attggcggga ttatgctttg gttaccgcca gggcgttggg
     9901 tcgcagaaaa agtcgctttt ggcgtccaca aagtgatggc gtacagcgac gcaggtagcg
     9961 catttatctt cggttctctg gtcggaccga aaatggatac cttatttgat ggcgcaggat
    10021 ttatctttgg tttcagggta ttaccggcaa ttatcttcgt cactgcactg gtgagtattc
    10081 tctactacat cggtgtgatg gggatcttaa ttcgcattct cggcggtatc ttccagaaag
    10141 ctttaaatat cagcaagatc gagtcattcg tcgcggtcac caccattttc ctcgggcaaa
    10201 acgaaattcc ggcgatcgtc aaacccttta tcgatcgtct gaatcgcaat gaattattta
    10261 cagcgatttg tagtggcatg gcctcgattg ctggttcgac aatgattggt tacgccgcac
    10321 tgggcgtacc tgtggaatat ttgctggcgg catcgttaat ggcgatccct ggcgggatct
    10381 tgtttgcccg cctgttaagc ccggctacgg aatcttcgca ggtttctttt aataacctct
    10441 ctttcaccga aacaccgcca aaaagcatta ttgaagccga tgcgacaggg gcaatgaccg
    10501 ggctgaaaat cgccgccggt gtagcgacag ttgttatggc atttgtcgcc atcattgcgt
    10561 taattaacgg tattatcggc ggcgttggcg gctggtttgg ttttgaacat gcctcgctgg
    10621 agtccatttt aggttacctg ctggccccac ttgcgtgggt aatgggtgtt gactggagtg
    10681 atgcaaatct tgccgggagt ttgattggac agaaactggc aataaatgaa tttgtcgctt
    10741 atctcaattt ctcaccctat ctgcaaacgg ctggcactct ggatgctaaa accgtggcga
    10801 ttatttcctt cgcgttgtgc ggtttcgcta actttggttc tatcggggtg gtggtggggg
    10861 cgttttctgc ggttgcgcca caccgtgcgc cggaaatcgc ccagcttggt ttacgcgcgc
    10921 tggcggcggc gacactttct aatctgatga gtgccaccat tgccgggttc tttattggtt
    10981 tagcgtagtg tgcaatttat gagccggatt agatgcggtc aagcgtcgaa tccggcaaca
    11041 ttgttacacc attggcacta atgacagcgc gttatcggca gacagggtag ggttgtttaa
    11101 gctgccgctg gcgcgtgaaa ttgccgcgca ggccatcgca aaacgggcgc tgtcacggaa
    11161 attgtttccc tccagaaagc tatataccag acccgccata aaaccatcgt cagcaccaaa
    11221 actgtctacc gtcgtatgcg ctggcacagt cagcaaaaat tgttcaccat ccttttcgct
    11281 gcaataaact gactcatcgg gcaaataaac aaacagttgc tgaacacctt gctgatgcaa
    11341 tgcattcact gcggcattac ggtcagcatc gctggtgatc gcctgtcccc ataaaatttc
    11401 cagctccggt aaagtgggtt tcagggtgtg aatatgcgcc agccagtgtt tgattttgcc
    11461 cgctttgaat tctgaaacgg tatcgacaaa caccgggatc tcatcagcga gggtaaagac
    11521 ccattccagc gcctcggctg tcaggttgca atcagccagt accacgcccg catgacgaag
    11581 taaatcgcgc gacccgttca gtagttgcgg tgtcaactgt tccagcagat gggtatcgtt
    11641 aattgccagc acggtttgat cgtctcgatt ggcaattgcc agatacgtcg atgtgctttg
    11701 accatgcaaa cgaacgcagc cggagacatt cacgccggca cggcgcgttt cttccaggag
    11761 catttcgcca taaaagtcat cgccaatcac tgaaagcaaa tggacgtcac ggcctaacag
    11821 cgccagattg tgggcgatat tgcgtccaac gccgcctgcc gaacaatgaa ttgtaccggg
    11881 atgagaagcc gcttgcgggt aatggatatc cgccatcccg cgaatatcca tattgattgt
    11941 ccccaccact acgcagtatt cctgctcggt gagaatgtaa cctttgtctt taatccggcc
    12001 tttgcgcatt aaatccataa tatgcgccgc aacgcgcgaa cgactgattt gcagcatgtc
    12061 cgcaatttcg ttctgctgaa tcagcgggtt acgccgtaaa attgcaagga tctccttttc
    12121 ccggttattc atcttcaggc taccgctttt tcagtttgtt gcgctttcag ccaggcgatc
    12181 tcttctgccc agatatccgg gttgatggtt tcaaggatca gcggaatgcc gtcgaaacgg
    12241 tcgttctgca taatccagcg gaacgcatca tgaccgatat tgccttcacc gaggctatga
    12301 tggcggtcaa cgcggctgcc aaaggtactt ttcgcatcgt taaggtgcat cccgcgcaga
    12361 tacttaaagc cgacaatacg ggcaaaatca gcgaacgttt tttcgcattc ggctggagta
    12421 tgcaaatcat acccggcagc gaaagcatgg caggtatcaa tgcagacgcc gacgcgggat
    12481 ttatcttcca cgccgtcgat aatcgccgcg agatgttcga atttaaaccc taagttactg
    12541 ccctgaccgg cggtgttttc aatcaccgct gttacacctt gagttttatc cagcgcaatg
    12601 ttgatggatt cggcaatacg cgcaaggcaa tcctcttctg aaatctgcat cagatggctg
    12661 ccagggtgga agttgagcaa agaaagcccc agttgttcgc aacgctgcat ttcatctata
    12721 aaggcatcgc gcgatttttc cagagcttca gtgaccggat gtccgaggtt gatcagataa
    12781 ctgtcgtggg gaagaatttg cgccgatgtg tagtgatatt tttcacaggc ggctttgaat
    12841 tcatcgatgg tttgcgtcgt gagcggtgcg gcacgccact gacgttggtt tttggtgaac
    12901 aaggcaaacg cggttgcgtc gatttcggcg gcgcgaattg cggcatttgc cagaccgcca
    12961 gcagcactaa cgtgcgctcc aatgtatttc atgcgaggac tcctgttaaa cccgctggag
    13021 gaaaacggta atgatagcgg gttaacagga gcaagaagta gtggtttatg cgatgacgct
    13081 ttgaatcaca tagttaatcg caccaccacc aacaatcagc caggcaaaca gtaccagtgc
    13141 catcagcaga ggtttcgccc cagctttttt cagcgcgctg acgtgagtgg tcagacccag
    13201 cgccgccatc gccattgcca gcaggaaggt atccagcgtt accagcatgt tcaccacgct
    13261 ctgcggtaac aggtggaacg agttaaagat ggcaactacg atgaacaaga tggcaaacca
    13321 cggaatagtg attttgcttt tctcgccgct gttcgcccca gacagctgtt taacacgcgc
    13381 cgccagcagg atgaggaacg gagccagcat catcacgcgc agcattttgg aaataactgc
    13441 tgcgttttct gcatccgggc tgatggcatg acctgccgcc accacctgcg ccacttcgtg
    13501 cacagtagaa ccaatgtaga taccgaaagt ttccggacta aaccattgag acatcagcgg
    13561 atatatcgcc gggtagagga aaatcgcgac ggtcccgaag ataacaacgg ttgcaacagc
    13621 cacggttact ttactggctt ccgctttcac taccggctca gtcgccagta ccgcggcagc
    13681 accacagata ctgctaccgg caccgatcaa ccaactggtg tgcttatcca gaccaaacac
    13741 tttctgcccc aggaagcaag ccagcaggaa ggtactggac agcgtcagca cgtcaatgat
    13801 gatcccactg ataccgacat cggcaatttg cgagaacgtc agacggaagc cataaagaat
    13861 gatacccaga cgtaataaat attgcttggc aaacagcaca ccaccgtcac agcttttcca
    13921 gatgtgcgga tagatggtgt tgcctaaaac catccccaac aagattgcga gggtgagggc
    13981 actaaacccg gcacccgcaa ccgcgggaat ggaaccaccc cacagggcga ccccggtgat
    14041 aactgcactc agggctaacc ccggaataaa atgccacagt gtacgatgtt gtttctgtaa
    14101 ggtgatattc gtcataaccc tctcctttaa ccgggcataa ggttacgact aactggttta
    14161 aaaataaaat tgattatata tttataatta atctttataa gtggtaagcg actatgcaca
    14221 tcaccctccg gcagttggaa gtttttgcag aagtattgaa aagtggatca accacccagg
    14281 cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc agccttgact gacctggaag
    14341 ggcagcttgg cgttcaactg tttgatcgcg tggggaaaag actggttgtt aatgaacacg
    14401 ggcggctgct ctatccgcga gcgttggcac tgcttgaaca ggcggttgaa atcgaacaac
    14461 tgtttcgcga agacaacggc gcgattcgta tctatgccag tagtaccatc ggtaactaca
    14521 ttctgcctgc agttatcgcc cgttatcgcc atgattatcc gcagttgccg attgaactta
    14581 gcgttgggaa cagccaggac gtgatgcaag cggtgctgga tttccgcgtt gatattggct
    14641 ttattgaagg accgtgccac agcactgaaa tcatttctga accgtggctg gaagacgagc
    14701 aggtggtttt cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc ttagaacagc
    14761 tggccgctgc gccgtggatc ctgcgtgaac gcggttccgg cacgcgggat attgtcgatt
    14821 atctgttgct gtcacattta ccgaagtttg agatggcgat ggaattaggt aactccgagg
    14881 caatcaaaca tgcggtgcgt catgggttgg gaattagttg cctgtcgcga cgtgtgattg
    14941 aagatcaatt gcaggcaggc acattaagtg aagttgcggt ccctctgccg cgcctgatgc
    15001 gtacgttgtg gcgtatacat catcggcaaa aacacctttc caacgcgcta cggcgctttc
    15061 tggactattg cgatcccgca aatgtgccgc gttaagttgc tgtacaagaa catgctggtg
    15121 ctgtgtcgat ttcgtgacgc agcgccttca gcatgcattc gccagaaaag agattggctg
    15181 ctttacttat aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga
    15241 aggattgcca atcgtctgct acaatcgcgc catcattttt aagatggata gcatttttgt
    15301 atgggttccg aaactaaaac tacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt
    15361 cacctgacga tgattgccat tggcggttcc atcggtacag gtctttttgt tgcctctggc
    15421 gcaacgattt ctcaggcagg tccgggcggg gcattgctct cgtatatgct gattggcctg
    15481 atggtttact tcctgatgac cagtctcggt gaattggctg catatatgcc ggtttccggt
    15541 tcgtttgcca cttacggtca gaactatgtt gaagaaggct ttggcttcgc gctgggctgg
    15601 aactactggt acaactgggc ggtgactatt gccgttgacc tggttgcagc acagctggtc
    15661 atgagctggt ggttcccgga tacaccgggc tggatctgga gtgcgttgtt cctcggcgtt
    15721 atcttcctgt tgaactacat ctctgttcgc ggctttggtg aagcggaata ctggttctca
    15781 ctgatcaaag tcacgacagt tattgtcttt atcatcgttg gcgtgctgat gattatcggt
    15841 atcttcaaag gcgcgcagcc tgcgggctgg agcaactgga caatcggcga agcgccgttt
    15901 gctggtggtt ttgcggcgat gatcggcgta gctatgattg tcggcttctc tttccaggga
    15961 accgagctga tcggtattgc tgcaggcgag tccgaagatc cggcgaaaaa cattccacgc
    16021 gcggtacgtc aggtattctg gcgaatcctg ttgttctatg tgttcgcgat cctgattatc
    16081 agcctgatta ttccgtatac cgatccgagc ctgctgcgta acgatgttaa agacatcagc
    16141 gtcagtccgt tcaccctggt gttccagcac gcgggtctgc tctctgcggc ggcggtgatg
    16201 aacgcagtta ttctgacggc ggtgctgtca gcaggtaact ccggtatgta tgcgtctact
    16261 cgtatgctgt acaccctggc gtgtgacggt aaagcgccgc gcattttcgc taaattgtcg
    16321 cgtggtggcg tgccgcgtaa tgcgctgtat gcgacgacgg tgattgccgg tttgtgcttc
    16381 ctgacctcca tgtttggcaa ccagacggta tacctgtggc tgctgaacac ctccgggatg
    16441 acgggtttta tcgcctggct ggggattgcc attagccact atcgcttccg tcgcggttac
    16501 gtattgcagg gacacgacat taacgatctg ccgtaccgtt caggtttctt cccactgggg
    16561 ccgatcttcg cattcattct gtgtctgatt atcactttgg gccagaacta cgaagcgttc
    16621 ctgaaagata ccattgactg gggcggcgta gcggcaacgt atattggtat cccgctgttc
    16681 ctgattattt ggttcggcta caagctgatt aaaggaactc acttcgtacg ctacagcgaa
    16741 atgaagttcc cgcagaacga taagaaataa gtttcctccc ttccttgcta agccctctca
    16801 accgagaggg ctttttcaat tccatttccc tgacaaatca tgcggatata aaatttaata
    16861 tttggattga taattgttat cgtttgcatt atcgttacga cacaatcaaa aaaggctgac
    16921 aaatcagagg ctgttccggc tttctgggat gatcacctgc atacaaataa gtccaccgcg
    16981 atgctgccgt acgcaagggg acgtaaagaa gatgtgagcg atacccattt tattttcgta
    17041 gttacctcat ggagatatgg aatgtttagg ttgaaccctt tcgtacgggt cgggctgtgt
    17101 ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg atgatgatgg cgaaacgatg
    17161 gttgtcactg catcttccgt ggaacaaaat cttaaagatg cacccgccag tatcagcgtc
    17221 attacccagg aagacctgca gcgaaaaccg gtacagaatc tgaaggatgt cctcaaagaa
    17281 gtgcctggcg tacaactgac gaacgaaggg gataaccgta agggcgttag tattcgtggt
    17341 ctggacagca gctacaccct gattcttgtc gacggtaaac gcgttaactc ccgcaatgcc
    17401 gtcttccgcc acaatgattt cgatctgaac tggatcccgg tcgattccat cgaacgtatt
    17461 gaagtggttc gtggcccgat gtcgtcgcta tacggctccg atgcgctcgg cggtgtagtg
    17521 aatatcatca ccaaaaaaat cggtcagaaa tggtcgggta ccgttaccgt cgataccacc
    17581 attcaggaac atcgcgatcg cggtgatacc tataacggtc agttctttac cagtggacca
    17641 ttaattgatg gtgtgctggg aatgaaagct tacggcagcc tggcaaaacg tgaaaaggat
    17701 gacccgcaaa actcaacgac caccgatacc ggagaaacgc cgcgtattga aggattctcc
    17761 agccgcgacg gcaatgtcga atttgcctgg acaccgaatc aaaatcacga ttttactgcc
    17821 ggatacggtt tcgaccgtca ggatcgtgat tccgattcgc tggacaaaaa ccgcctggaa
    17881 cgccagaact actgcgtcag ccataatggg cgttgggatt acggcaccag cgaactgaaa
    17941 tcctacggtg agaaagtcga gaacaaaaac cctggcaaca gcagcccgat aacttccgaa
    18001 agcaatacgg tcgacggcaa atacacgttg ccgctgacgg cgattaatca gtttctcacg
    18061 gttggcggtg aatggcgtca cgacaaactt agcgatgcgg tgaacctgac cgggggaacc
    18121 agctccaaaa cgtctgccag ccagtacgcg ctgtttgtgg aagatgaatg gcggatcttc
    18181 gagccgctgg cgctgacgac cggcgtgcgt atggacgatc acgaaaccta cggtgaacac
    18241 tggagtccgc gtgcctacat ggtttataac gccaccgaca ccgtaacggt gaaagggggc
    18301 tgggcgacgg catttaaagc accttctctg ttgcaactta gccctgactg gacgagcaat
    18361 tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc tgaaaccaga aaccagcgaa
    18421 agttgggagc tggggcttta ctacatgggt gaagaaggct ggctggaagg gggtgaatcc
    18481 agcgttatcg ttttccgtaa cgatgtgaaa gatcgtatca gcatcagccg tacgtctgac
    18541 gtcaacgctg caccgggcta ccaaaacttt gttggttttg agacgggcgc taacggacgg
    18601 cgcataccgg tatttagcta ctacagcgtt aacaaagctc gtattcaggg cgtggaaacc
    18661 gaactgaaaa ttccgttcaa cgatgaatgg aaactgtcga tcaactacac ctacaacgat
    18721 ggtcgtgatg tcagcaacgg cgaaaacaaa ccgctatccg atctgccgtt ccatactgct
    18781 aacggtacgc tggactggaa accgctggcg ctggaagact ggtcattcta tgtttctggt
    18841 cactataccg ggcagaaacg cgccgacagc gcgacggcta aaacaccggg cggttatacc
    18901 atctggaata ccggcgcggc ctggcaggtg actaaagacg tcaaactgcg cgcaggcgtg
    18961 ctgaaccttg gcgacaagga tctcagtcgt gacgactaca gctataacga agacggacgt
    19021 cgttacttta tggcagtgga ttatcgcttc tgatgagaag atgcccggcg aaccgggcgg
    19081 actttcactt cagtaaatac tgcgcatgga agcgcaggtg atcctctata aaagaggcga
    19141 tgaagtagta actgtgatca tatcccggct gaatacgcag cgtcatcggc cacgctttct
    19201 ggcgcgcggc ttctgccaat accgcaggtt gcaactggtc ggcaagaaac tgatcattat
    19261 cgccctgatc gataagcgtc gggatcgcat cctgcgcgtt actggcatac atcagcgcgc
    19321 aactatccca ttccagcatg catttttgtc ctcacctaaa taggtgctaa acgctttgat
    19381 tccccacggg acgctgcacg gattcacaat gggcgcaaag gccgaaacgc tggtgtattt
    19441 acccggattt ttcagcgcca taatcagcgc accgtgacca cccattgagt gaccgctaat
    19501 ggcgcagcgg tcgctgacat taaattgcga ctgaaccagc gccggtaatt catcgcgcag
    19561 ataatcatac atccggtaat gcgtcgccca cggcggttgc gtggcattaa gataaaagcc
    19621 tgcgccctgg cctaaatcgt agccatcgtc gttggcaacc ttttcgccgc gcgggctggt
    19681 gtctggcatc accagtacaa tccccagttc cgccgctacc cgctgggcac ccgccttggt
    19741 ggtgaagttc tcgtcattgc aggttaatcc ggaaagccag tacagcactg gtggcggggt
    19801 gtgatcacga gggggtggga gaaagatact gaacgtcatc gggcagttta aggtactgga
    19861 gtcgtgtcgc cagcgttgct gccagccttc aaaacagcgg tgctcttcga gcatttccat
    19921 gcgtggctcc ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt caatgtgctg
    19981 tgagtaactt tcacttccgt atttgcataa cgatgtttta acatctgctg atgaaaggca
    20041 gcggcaatta caataattat cgctgtgaat actggattat gtgcgccgcc tcacgcacaa
    20101 taatcaagct gtaaatcagc ttaataactt tgcccccacg cagggcggag gcgtcacacc
    20161 tgcaggagaa atcataaatg ccatcactca gtaaagaagc ggccctggtt catgaagcgt
    20221 tagttgcgag aggactggaa acaccgctgc gcccgcccgt gcatgaaatg gataacgaaa
    20281 cgcgcaaaag ccttattgct ggtcatatga ccgaaatcat gcagctgctg aatctcgacc
    20341 tggctgatga cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat gtcgatgaaa
    20401 ttttctccgg tctggattac gccaatttcc cgaaaatcac cctcattgaa aacaaaatga
    20461 aggtcgatga aatggtcacc gtgcgcgata tcactctgac cagtacctgt gaacaccatt
    20521 ttgttaccat cgatggcaaa gcgacggtgg cttatatccc gaaagattcg gtgatcggtc
    20581 tgtcaaaaat taaccgtatc gtgcagttct ttgcccagcg tccgcaggtg caggaacgtc
    20641 tgacgcagca aattcttatt gcgctacaaa cgctgctggg caccaataac gtggctgtct
    20701 cgatcgacgc ggtgcattac tgcgtgaagg cgcgtggcat ccgcgatgca accagtgcca
    20761 cgacaacgac ctctcttggt ggattgttca aatccagtca gaatacgcgc cacgagtttc
    20821 tgcgcgctgt gcgccatcac aactgattaa aaggcaggaa ccatggagcg caacgtcacg
    20881 ctcgattttg ttcgcggcgt cgccattctg gggatcctgc tattaaacat cagcgccttt
    20941 gggctaccaa aggcggctta tctcaatccc gcctggtacg atgctattac gccgcaggat
    21001 gcatggacct gggcatttct cgatctcatc ggccaggtga aattcctcac gctttttgcg
    21061 ctgctgtttg gtgctggcct gcaaatgttg ctgccccgtg gcagactctg gatccagtcg
    21121 cggttaacgc tgttagtctt gttgggcttt attcacggtt tattgttctg ggacggcgat
    21181 attctgctgg cttacgggct ggtggggctt ggcgttttgc tgttattggg gctgatttcc
    21241 gacagccaga ccagccgcgc ctggacgccg gatgcatcgg ctattttgta tgaaaaatac
    21301 tggaagcttc acggcggcgt tgaagcgatc agtaatcgtg ccgatggtgt tggcaacagt
    21361 ttactggcac tgggcgcaca gtatggctgg caactggcag ggatgatgct cattggtgcg
    21421 gcgctgatgc gcagcggctg gctgaaaggg cagttcagct tacgtcacta tcgtcgtact
    21481 ggttttgtgc tggtggcgat tggggtgacc attaaccttc ctgccatcgc cctgcaatgg
    21541 cagctggact gggcgtatcg ctggtgtgcc ttcttacttc aaatgccgcg ggaactgagt
    21601 gcgccgtttc aggcgattgg ctatgcgtcg ctgttttatg gcttctggcc gcaattgagc
    21661 cgctttaagc tggtgcttgc gatcgcctgc gtcggacgga tggcgctgac caactatcta
    21721 ttgcaaacgc tgatttgtac cacgcttttt taccacctcg gtttgtttat gcattttgac
    21781 cgcctggagc tgctggcgtt tgttattccg gtatggctgg cgaatatcct cttctctgtt
    21841 atctggctgc gttacttccg ccaggggccg gtggaatggc tctggcgtca gttaactttg
    21901 cgtgctgccg gaccggcaat atctaaaaca tcaagataac gatctggatt acattcgtta
    21961 acaaaacggc tgtaaccgtt tccattgctg tgactcgatt cacgaagtcc tgtattcagt
    22021 gctgacaaaa tagccgccag caagcagtca tttactgcaa tctcataaca ggtagtgaat
    22081 atgatcacca ttcgtgatgt agcgcgtcag gctggcgtct ctgtggcaac ggtttcccgg
    22141 gtgctcaata acagcacgct ggtcagtgcc gacacgcgtg aagcagtaat gaaagccgtg
    22201 agtgagctgg attatcggcc aaacgccaat gcccaggcgc tggcaactca ggttagcgac
    22261 accattggcg tggtggtgat ggacgtttct gatgcgtttt tcggcgcgct ggtaaaagcg
    22321 gtggatctgg tcgcccagca gcatcagaaa tacgtgctaa tcggcaatag ctatcatgaa
    22381 gcggaaaaag agcgtcacgc cattgaggtg ttaattcgcc agcgttgtaa tgcgttgatt
    22441 gttcactcaa aagcattgag tgacgatgaa ctggcgcaat ttatggataa cattcccggt
    22501 atggtgttaa tcaaccgcgt tgtgccgggg tacgcccatc gttgcgtttg cctggataat
    22561 ctcagcggtg cccgaatggc gacgcgcatg ttgctgaata acggtcatca acgtattggt
    22621 tatctttctt ccagccacgg cattgaagat gacgccatgc gtaaagcagg ctggatgagt
    22681 gcgttgaaag agcaggatat tattccgccg gaaagctgga ttggcactgg tacgccggac
    22741 atgccgggcg gtgaggcggc gatggttgaa ctgctggggc gcaatctaca acttaccgct
    22801 gtatttgctt ataacgacaa tatggccgct ggcgcactga cagcattaaa agataatggc
    22861 attgcgattc cgttacatct ctcaatcatc ggtttcgatg atattcccat cgcccgttac
    22921 accgacccgc aattaacgac cgtgcgttat cccattgctt caatggctaa attagccacc
    22981 gaactggcct tgcagggggc agcaggcaat attgatcctc gtgccagcca ctgttttatg
    23041 ccgacgttag tgcgtcgcca ttctgtcgca acgcgccaga atgcggcggc gatcactaac
    23101 tcaacaaatc aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca
    23161 atgtgatagc tatgattgca ccgttttaat gttgtaaccc gtatgtaaca gtgaataatc
    23221 actttcaccg aggtaatagc gtcataacaa caattaaagc cgttttctgg agcgttaccg
    23281 ggcatggaag aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc
    23341 tactgccccg ccgagcattt atctcaagca ctaccctgca taagaaaaac cggagatacc
    23401 atgaataaga aggtgttaac cctgtctgct gtgatggcca gcatgttatt cggtgccgct
    23461 gcacacgctg ctgatactcg cattggtgta acaatctata agtacgacga taactttatg
    23521 tctgtagtgc gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg
    23581 atgaatgatt ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg
    23641 aaaggggtga aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt
    23701 gagaaagcgc gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc gtctcgtaag
    23761 gcgctggata gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt
    23821 attcagggcg atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa
    23881 gacggtcaga ttcagttcgt actgctgaaa ggtgaaccgg gccatccgga tgcagaagca
    23941 cgtaccactt acgtgattaa agaattgaac gataaaggca tcaaaactga acagttacag
    24001 ttagataccg caatgtggga caccgctcag gcgaaagata agatggacgc ctggctgtct
    24061 ggcccgaacg ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat ggcaatgggc
    24121 gcggtagaag cactgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgatgcg
    24181 ctgccagaag cgctggcgct ggtgaaatcc ggtgcactgg cgggcacagt actgaacgat
    24241 gctaacaacc aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggcaaaggt
    24301 gcggctgatg gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc
    24361 gtagataaag acaacttggc tgaattcagc aagaaataag actgattggg cgcagtcgtt
    24421 tactgcgccc ttattgtatg gccaacaagg taaaattata tggtcagctc aacgactccg
    24481 tcttccgggg aatacttgtt ggaaatgagc ggtatcaaca agtcctttcc tggtgttaag
    24541 gcacttgata acgttaattt aaaagtccgg ccacattcta tccatgcatt aatgggggaa
    24601 aacggtgcag gaaaatcgac attattaaaa tgcctgtttg gtatttatca aaaagactcc
    24661 ggcaccattt tattccaggg taaagagatc gatttccatt ccgccaaaga ggcactggaa
    24721 aatggtattt cgatggtaca ccaggagtta aacctggtat tacaacgttc ggtgatggat
    24781 aacatgtggc tggggcgata tcccaccaaa ggcatgtttg tcgatcagga caaaatgtac
    24841 cgcgaaacca aagcgatttt tgatgaactg gatattgata tcgatccgcg tgcgcgcgtc
    24901 ggcacattat ccgtttcgca aatgcagatg atcgaaatcg ccaaagcgtt ttcctataac
    24961 gcgaaaattg tgattatgga tgaaccgact tcttcgttaa ccgaaaaaga ggtcaatcat
    25021 ctgttcacta ttattcgtaa attaaaagag cgcggctgcg gtattgttta tatctcgcat
    25081 aaaatggaag aaatcttcca gttatgtgat gaagttaccg tattgcgcga cggtcagtgg
    25141 atcgccaccg aaccgctggc aggactgacg atggacaaga tcatcgccat gatggttggg
    25201 cgttctctta atcagcgttt tcctgacaaa gaaaacaagc cgggcgaagt catcctcgag
    25261 gtgcgtaacc taacgtcact gcgccagccg tcgattcgcg atgtctcgtt tgatctgcat
    25321 aaaggggaga tcctcggtat tgccgggctg gtgggggcga aacgtaccga tattgttgag
    25381 acgttatttg gtattcgcga gaaatcggct ggcaccatta cgttgcacgg caaacagatc
    25441 aataaccata atgccaacga agccataaac cacggatttg cactggtaac tgaggagcgc
    25501 cgctcaacgg gaatttatgc ctatctggat attggtttta actcgttaat ttccaatatt
    25561 cgcaactaca aaaataaagt tggtttactg gataactcgc ggatgaaaag cgatacccag
    25621 tgggtgattg attcgatgcg ggtaaaaacg ccgggtcatc ggacgcaaat tggttcgctc
    25681 tccggtggta atcagcaaaa ggtgattatt ggtcgctggc tactaacgca accagaaata
    25741 ttaatgctcg atgaaccgac gcgcggtatt gatgtcgggg cgaagtttga aatttatcag
    25801 ttaattgccg aactggcgaa gaaaggcaag gggattatta ttatctcctc tgaaatgcct
    25861 gagttgttag ggataacaga ccgtattctg gtcatgagca atggtctcgt ttccggaatt
    25921 gtcgatacaa aaacaacaac gcaaaacgaa attctgcgtc ttgcgtcttt gcacctttaa
    25981 gattaggggc ttcccatgag tgcgttaaat aagaaaagtt ttcttactta cctgaaagag
    26041 ggcggtattt acgtcgttct tttagttttg ctggcgatta ttattttcca ggacccaaca
    26101 tttttaagtc tgttgaactt aagtaatatt ctcacccagt catcggtgcg tattattatc
    26161 gcgctcggtg tggcagggtt aattgtcacc caggggaccg atctttctgc tggtcgtcag
    26221 gtagggctgg cggcagtggt ggctgcgaca ttattgcagt ccatggataa cgccaacaaa
    26281 gtgttcccgg aaatggcgac gatgccgatt gcgctggtta ttctgattgt ctgtgccatt
    26341 ggtgcggtga tcggtttgat caacggtctg attatcgctt atctcaacgt gacgccgttc
    26401 attaccacgc tcggcacgat gatcatcgtc tatggcatca actcgctcta ttacgacttt
    26461 gtcggggcgt cgccaatttc tggttttgac agtggcttct ctacctttgc tcagggcttt
    26521 gtcgcgctgg ggagtttccg tctctcttac atcaccttct acgcgttgat tgcggtggcg
    26581 ttcgtctggg tgttgtggaa caaaacccgc ttcggtaaga acatttttgc cattggcggt
    26641 aacccggaag cggcaaaaat atctggtgtc aacgtcggcc tgaacctgct gatgatctac
    26701 gcgttgtctg gcgtgttcta tgcctttggc gggatgttag aagccggacg tatcggctct
    26761 gccaccaaca acctcggctt tatgtatgag ctggatgcta tcgcggcgtg cgtggtaggc
    26821 ggtgtatcgt tcagcggcgg tgtggggacg gtgattggcg tggtgaccgg ggtaattatt
    26881 tttaccgtca tcaactatgg cctgacgtat atcggcgtaa acccatactg gcagtacatc
    26941 atcaaagggg cgattattat cttcgccgta gcgctggatt cactgaaata cgcgcgtaag
    27001 aagtgatgtg attgcggata ttgccggatg cgtctctggt tttacaacgt taccgggtgt
    27061 tctttctcgc ctttcttaaa cttcacttcc ccgagctcaa tacaacccac cgggcagacg
    27121 tgaccacaaa gcagacaacc cacacatttc tcggtattac aatgcggtgt gcgggttttc
    27181 tcgctccatt ccatcgcctg gtgaccgccg tcgtaacagg aaatataaca gcgtccacag
    27241 ccaacacatt tgtcaagatt gatacgggga tagacaatat aactgcggtc taaatcttcc
    27301 gccgggacaa tattgttatt cgccagacct accatctcct gcaacgaatc aaatccctga
    27361 tcggcgagat aatgcgataa cccgctcgcc atatcttcta cgatgcgata gccgtactgc
    27421 ataatgccgg tggtgacctg taacgttgct gcgccgagca ataaaaactc cgccgcatct
    27481 tcccaggttt caatgccgcc gataccgctg attgggaaat cgcgcagttc tggatgggtg
    27541 cgcatttgct gaatgaagcg cagggcgatc ggttttaccg ctttaccgga atatccggaa
    27601 atactcgatt taccgttaac gatcggcata ccgattttct gattaagatc gatattggtg
    27661 atggatttaa cggtgttaat tgccgcaatg ccatctgcgc cgccgcgctt cgccgccagc
    27721 gccacttcgc acatatcacc gatattcggc gtcatcttcg ccagcattgg cagcgtggaa
    27781 ccccgtttca ctgcccgaca atatttttct accagctccg ggctttgccc gacatcgcta
    27841 cccatcgcat gagaagtcat ttgcggacag gagaagttac actcgatcat atccgcgcca
    27901 gctgctccca ttgctgctca ttttccccca tgatcgaagc gatcaatact ttgtccgggt
    27961 aatcttcctt cagccgacgc agggcggtca gattctcttc caacggatgt tcagcaatct
    28021 gctccatatt tttgaagccg ataaaaccgg tatcttcttt caccagatga tcaaaacgcg
    28081 gcgagacttc gttggcgata aaaaagccga tcgttttaaa caccacaccg ccccaacctg
    28141 tgtcgtaggc tttggcacac atctcatagc agttgcctac cggcgaagaa gagaggcaga
    28201 acgggttggg aaagttcacg ccgcaaaaag taatcgaaag atctttcgtt aacatgagca
    28261 agctccctct aaatagtgat gaatcgcctc ggcggcttct ttcccggttt tcacggcata
    28321 gaccacggtt ttgtcaccct caacaatatc gccagcagca aagacttgcg gatcgcgggt
    28381 ctgataattt tgtgttttga tggtgttacg ctgctgctct aactccgcaa aggcatccag
    28441 tctggcatgc tgaccgacgg cgagaataat tttatctgcc gccatcgtca gttcgcctga
    28501 taaccgtaca tgcttaaacg tgactttatt gccttccacg gctactggcg tgaatccatc
    28561 gatgatcgaa acgcccagct cccgggcgct ggcaaactct ttttcgcttg ccggaaactc
    28621 atctaactct tcacgtgcta cgcaagttac cgcctgacag ccgagaactt tcagcgtgct
    28681 ggctacgtcc atcgcgacat caccgccgcc gataattaat gcgctttgtg gaatgctgat
    28741 atcgccttgc gcctgtcgta cacgttgcag gaagtcgacg gcaatctcaa cgtcgctatg
    28801 ctcaaacagc ggtagcccgg aaccgcttga taaccccacg gtgaccagta ccgcgcggtt
    28861 ttccgctttc agttgctcca gggtgagtgt tttaccgatt tcgttattgc acttaatggt
    28921 cacgcccatt ttttcaatac gggcgatctc tgcatccagc actgattgcg gtaaccggaa
    28981 ttgcggaata ccgttacgca accagccacc ggggtgcgct tctttctcat aaatcgtgac
    29041 gtcataaccc tggtttgtca gtgtcacact ggcctgcaat ccggcaggac ctgcgccaat
    29101 aatcgcgact ttgccgagcg ttttagtacc gggctgataa atttccatcc cggtttgttg
    29161 ttcaaaatca gtaacaaaac gctgtaagcg gccgatatca atgggagcat caacaccggc
    29221 acgggtacaa ccgctttggc ataatttctc cgtcgggcac actctggcac aaacggcacc
    29281 gagggcatta ttttcgcgaa ttgtctcggc agcgccttta aaattacgaa agtagattga
    29341 gcgaataaat ttccccggat cggtctgtgc cgggcaagcc tgactacagg gagcgtcgtg
    29401 acataataaa cagcgagagg cttctttaat cgccagtaac ggcgtaaaag ccggagtgag
    29461 ttcatccaga taattttgtt gcggcatgtg atcttcctta atgctatgag taattatggc
    29521 gaacgctaaa taaatattgc gtgctgatat taaggctatg cgcgtgggga aatttaagct
    29581 gtgaaaggat tcacaaatag ctgcggttca gaatgttata tccgcagctt attgagtgaa
    29641 gtaataatta ttctcaataa ttaacagaaa agagaaaata ttaacgatag aaaataagat
    29701 aaacaatcat gccgatagat aaaattccga agccaatata tgccaccata taagcgaaga
    29761 agagtccggg attttttgct cgccagacat acaccggttc cggtgcattt ttaacccgtt
    29821 tatcaatcgc gcctgcgcgc cagcctttcc atgcttcgcg gaaacaaaat atcgggataa
    29881 ggaaaaaaac ggcaacgaca aaaaactgct gtacatccat aactactttc cttgtttctt
    29941 ttgtaattca agtaactgtt cgggtgtaac ggcgggatac ccctgcgcat cttccggcac
    30001 ctggtgcata gccggaatag ggaccagcgg ccctaaaaaa cgcggttcac gtttaaaaat
    30061 ataaaggtca gccagcgcac cgaaacgggc ggcaaattcg cgaatacgta ctgacagcat
    30121 atctttcggt gacggtacgg cataacactg agcctgaatc cccatatgca gcgcaataaa
    30181 taatgctcgc tcacagtgga aacgttgggt gataataatg aaatcattag tatcgaaaac
    30241 tttgcgtgta cgcacgatgg aatccagcgt acgaaagcct gcgtaatcga gaacaatatc
    30301 tgatgggtcg acaccagcag cgattaaatc tttgcgcatg gtcatcggct cgttataact
    30361 ttgcaatgcg ttatcgccgc tcagtaatag ataatttacc ttaccgctgt tataggcatt
    30421 aatcgctcct tgaatgcggt agcgataata ctgattaata acgccggtac gataatattt
    30481 tgctgtgccg agcaccacgc cgacctggcg gtaggggaga tcctgcaatt cgtcgtagat
    30541 ataaggcgcg gttttccagc tcatccagcg atcgaggccg agcacagtca acagcagcaa
    30601 gccgatcagg actaacaggc tgaggaacac gcgctttaac atgcaggaga ctcgagctaa
    30661 ggaaaatttc catcaggcta ctttacccgc tggttttgcg caagaaacgg caaactgttt
    30721 gccgggaatt tcgacaccag gagagggctg gcaagccgga caaggcgttc acgccgcatc
    30781 cggcaccagg cttaagcgag aagcacgcgg tcgatactgt gacagccgag agctttcagc
    30841 gttgcggagg tggcatccca ctgaatcaac ggcgcatcgg ctttttctgc cagaaccgcg
    30901 cgctggatat ccgggtaatc ataacccttg agattcaaca gaattaacgc cccctgcaac
    30961 ggcggcagag tcgggttgaa tgcggcgttt tcagcgtagc tgccgctgaa aatacgaccg
    31021 tctttacatt ccagcgcgac accgcttggt gacttactgt aaggcatgtg cgaacggttt
    31081 gccgctgcaa tcgccgcctg agaaagcgca tcaccagtca gcgcatagcc gtgatcctgc
    31141 tcgtccatca gcagcgtttt aatctccaga tctttcggcc caaaggcatc cggcagatag
    31201 tcacgcagcg cgtgtgcctc gcggcccggc aaatgaatac gcagatccag accgctgttc
    31261 agttcattca taaactgacg gcagtgacca caaggcgtat agttaacggt gatggctgca
    31321 agcgcttttt caccactcaa ccaagcgtgg ctgattgcgc tttgttcggc atgaacggtt
    31381 tgctgcattg ttgcaccaat aaattccata ttggcaccaa aataccaggt tccgctcaca
    31441 ccgcgcgcaa ttgcgccaac attaaaattc gacaatggcg tacgcgcaca ggccgccgcc
    31501 agcggaagta gtgcgaatgc cagcgcgtct tcgtccagtc ccgttgcgct cttcagcgat
    31561 gagacttgct ccccggtcaa caaagcgggg aagtacttgt ctgccagaat aggttccagt
    31621 gcagattgca agttatccgc aagttgggca aaagcggttt gaaaacgtgg atgcatggcg
    31681 ttgcctcata atcatgtaat gggataacag tttacgaacc cgttatggct ttatatgtga
    31741 tctgaatctc attaattatg caacacgtga aacttacatc aaaaatgcgc gacgtatcgc
    31801 aaattttagc ccattactgc cagaataatc gggaaaagga acggtgcgat cagcgaagta
    31861 attatcccgc ataacaccag cgctagcgaa ctaaatgcac cttcctgata atccagctcg
    31921 gcgcagcgcg ccgtaccgag ggcgtgcgag gcagtcccca ttgccagacc gcgcgcagct
    31981 ttggtacgaa tacgcatcgc attaagcaat gtatggccaa ataccgcgcc gaggatgccg
    32041 acgaaaatca cgcaaactgc gctgattgcc ggaataccgc caatactgcc gccaaccgcc
    32101 attgcaatag gcgtggtgac tgatttcggc aggattgacg cggcgatttc cggtgaagcg
    32161 cccatcaaca atgccacgga agtccccgtc accatcgcaa ccacgctgcc gataaaacag
    32221 atggtgatga tcgatttcca gcgcgcgcgg atctggtgta gctgctcata taaaggatag
    32281 gccagcgcga cgaccgccgg ttgcagcaga tcgtttagca cttcgctacc tttaaagtaa
    32341 ctgtcgtaag agatgccggt cagcattaaa aaaggaatga tcaccaccat tgctaccagc
    32401 aacgggttaa gcaggggaaa cttataccgt gccgccagtt tgcgggcggc aaaaaagaca
    32461 atcaacgtca gcggtaatga ccaccagata ttcgccatca ttcttctgat cctttctgac
    32521 ctacgacttt acgttcaccg tgtaccagtt gtgaactcca gctcaccacc agaaaaacca
    32581 ccagcgtact gacagcacag gaaaccacta ccgggccaaa ctgcgcgcgg agcaaatcaa
    32641 aatattgcat gacgcctacg ccaatcggca caaacaatag cgccatatag cgaatcagta
    32701 cgtagcaccc cggattgacc catttcgccg gaagaatttg caaggccagc aggacaaaca
    32761 ggatctgcat cccgatgatg ctgcccggaa tggttaccgg tagcagggaa gcaatgaaaa
    32821 tgcctgcata caggcaggca taaatcagga tgaaagcgcg taaatattgc cagataatgt
    32881 tcagtgtctt actcataatc aacaccccag atgagatgga ttcatcatac aattaattcg
    32941 taaaacgtgc taccgatcac attaagaaat cagcgcttat tttttcgcta cccggtatta
    33001 cgcaatgagc agcgaactca ggcatacatc aagcatagag agggattgca gtttgcagca
    33061 atccctcttt gcagaccttt aatccgctga ccataggcgc taacatagcg cctcattttt
    33121 ttgcgggtga tgatatgcgt gtgttactgg caccgatgga gggagtgctt gactctcagg
    33181 tgcgtgaatt gctgaccgaa gttaacgact acgatctgtg catcaccgag tttgtccgcg
    33241 tggtggatca actgctgccg gtaaaagtct ttcatcgcat ttgccctgag ctacaaaacg
    33301 ccagccggac accatctggt acgctggtgc gcgtgcagtt gttaggtcag ttcccacaat
    33361 ggctggcaga gaacgccgcc cgtgcggtgg agttaggttt ctggggcgtg gatctcaatt
    33421 gcggctgccc gtcgaaaacg gttaacggta gcggcggcgg ggcgacgtta ctcaaagatc
    33481 ctgaactcat ctaccagggg gcaaaagcga tgcgtgaagc tgtaccggcg catttgcctg
    33541 tcagcgtgaa agtgcgtctg ggctgggaca gcggtgagaa gaaatttgaa atcgccgatg
    33601 cggttcaaca ggctggcgct acggagctgg tggtgcatgg gcggacgaaa gagcagggtt
    33661 accgcgcgga gcatattgac tggcaggcga ttggcgagat tcgccagcgg ctgaatattc
    33721 cggtgattgc caacggtgaa atctgggact ggcagagcgc gcaacaatgc atggcgatca
    33781 gcgactgcga cgcagtgatg attggtcgcg gggcgttcaa tattcccaac ctgagccggg
    33841 tggtaaaata taacgaaccg cgaatgccgt ggccggaggt ggttgctttg ctgcaaaaat
    33901 atacccgtct ggaaaagcag ggcgataccg ggttatatca cgttgcgcgg attaaacagt
    33961 ggttgagtta tttgcgtaaa gaatacgatg aagcaacgga attatttcag catgttcggg
    34021 tgttgaataa ctcccctgat attgcaaggg ctattcaggc aattgatatc gagaaactct
    34081 aaaaaccata gtgatgtttt tgacaatgtg ttagctcttt atcaataggc cgcacggttg
    34141 tttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc acatggatgt
    34201 gggctgaggg cgcgttttac agggatgtta cctcgcgccc gacccggtag ccgtaaggga
    34261 taagccgagg gcaccgcgca gcggcgattt tgttcgccag agcccggggg ggcagggggc
    34321 ggcggcgatt ggtcgccccc tgcgcgctcc ttgcgccagt gattatatga agcaatgcac
    34381 atgacaggag cgcaacagga tgagagactg aaatattctc gttattttcg gatctgatga
    34441 cattaattaa ttttaaaata tcatcttaaa tacgccagtc accaccagta gcccaatcag
    34501 gaaaataatc aatacagccc agagtataat tttcattggc gttcctttat ttgtgagtgc
    34561 tgaataagta taggaaaaaa cgaagtcctt tgccgttatc cataaacgat taagataaat
    34621 cggaagatat catttactta gtctatattt ttctgggtat gtcgttttat tgctactgat
    34681 gtattgccac gcgaaactaa aaaatatatc gcaacacacg gtaatctccg cgcaccgttt
    34741 cttacctgat tattccccca tgaatcgtga ttccttttat ccagccatcg cctgttttcc
    34801 gctgttactg atgctggccg ggtgtgcgcc tatgcatgaa acccgccagg cgttaagcca
    34861 gcaaacgccc gctgcacaag ttgacaccgc attacccacg gcgctgaaaa atggttggcc
    34921 agacagccaa tggtggctgg agtatcacga taatcagctc acttcattaa ttaacaatgc
    34981 actacagaac gcaccggata tgcaggtggc tgaacagcgc attcagctag ctgaagccca
    35041 ggcgaaggca gttgccactc aggatggtcc gcagatcgac ttttcggcgg atatggagcg
    35101 gcaaaaaatg tcggcagaag ggctaatggg gccgtttgct ctgaacgatc cggccgcagg
    35161 tacgaccggt ccgtggtaca ccaacggaac ttttggctta acggcgggct ggcatctcga
    35221 tatctgggga aagaatcggg cggaggttac tgcccgcctg ggtacggtta aagcacgggc
    35281 ggcggaacgc gagcaaaccc gccaattgct ggctggcagc gtagcccgcc tgtactggga
    35341 gtggcaaacc caggcggcgt taaacacggt cttgcagcaa atagaaaaag agcagaacac
    35401 cattatcgcg accgatcgcc agctatatca gaacgggatt acttcttcag ttgacggtgt
    35461 ggaaaccgat attaatgcca gcaaaacccg gcagcagctc aacgatgtcg cggggaaaat
    35521 gaaaattatt gaggcacggt taaatgcact cacaaatcac cagactaagt cattgaagct
    35581 taaaccggtc gcgttgccga aagtggcaag ccagcttcct gatgaactgg ggtactcctt
    35641 actggcccgg cgggcagatt tgcaggcggc gcactggtac gttgagtcat cgttaagcac
    35701 tattgatgcg gcaaaagcgg cattttatcc tgacatcaac ctgatggcct tcctgcaaca
    35761 ggatgcgttg cacttaagcg atctgttccg tcattccgcg cagcaaatgg gcgttacggc
    35821 aggcctgacg ctacctattt tcgatagtgg tcgtcttaac gccaatctcg atatcgcaaa
    35881 agccgaaagc aacttgtcta tcgccagcta caacaaagcg gtggttgaag cagtgaatga
    35941 cgtggcgcgg gcagccagtc aggttcagac actggcggag aaaaaccagc atcaggcgca
    36001 aattgagcgc gatgccttgc gtgtggtagg tcttgcgcag gcccgcttta acgcgggcat
    36061 cattgctggt tcccgcgtca gcgaagccag aatcccggcg ctgcgtgagc gggccaatgg
    36121 cctgttattg caagggcagt ggctggatgc ctccattcga ctcaccggtg cgctgggcgg
    36181 gggttacaaa cgctgatgat atgcgccttc tatacttaac gtttattcag cgttaagtgg
    36241 agaactcgat ggcataggtc gcgataatta ccgcttccga ttcggggatc ggcaaagagt
    36301 gcgcgttatt actggcgcag caggggtttg atattggcat tacctggcac tcagatgaag
    36361 aaggggcaaa agataccgcg cgtgaggtgg ttacccacgg cgtacgtgcg gagatcgtgc
    36421 aactggatct cggcaatctg ccagaaggtg cgcaggcgct ggagacactc atccagcgat
    36481 ttgggcgtat tgatgtgctg gtgaataatg cgggtgcaat gaccaaagcg ccgtttcttg
    36541 atatggcttt tgatgagtgg cgcaagattt ttaccgttga tgttgatggt gcatttttat
    36601 gctcgcaaat tgcggctcgt cagatggtga aataagggca gggcggtcgc atcatcaaca
    36661 ttacgtcggt acatgaacat acgccgctgc cggatgccag cgcctacaca gccgctaaac
    36721 atgcgctcgg cgggttaacc aaaacgatgg cgctggagtt ggtcaggcat aagattttgg
    36781 tgaacgcagt cgcgcctggg gcgatagcca cgccaatgaa cagcatggat gatagcgacg
    36841 tgaagcccga cgcggagcct tcgattcctc tacggcgttt tggcgcaacg catgagattg
    36901 ccagcctggt ggtgtggctt tgttcggagg gcgcaaatta cacgacaggg cagtcgttga
    36961 tagtggatgg cggctttatg ttggcgaatc cgcagttcaa cccagaatag cgcagtgagg
    37021 tgcgtggata tttcaatggg ttaggccaga taagacacca gcgtcgcatc aggcatggaa
    37081 cacccggtgc cggatgtgga gtgaatgctt atctgtggca actgctttac gcctgattat
    37141 ccggcttttt cttcccgcgc cgtttcagcc accagcgcac gcccaccacc agaaccacga
    37201 ccagaatcaa ccagacccag tgcttcaaat gctggtcgag attgtgcaac cacggcgcaa
    37261 tcacctgacc accagcgtaa ccaatagtgg taaaaatcaa cgcccaggca aatgcgccga
    37321 gaatattcag cggcagaaag attttcggcg gcagctggct ggcaccaatc agcgtcgggc
    37381 caatcacccg aaagccatac ataaagcgcg taccaatgac aaacagatac ggatggcgtt
    37441 ggataagttt ctgtgcccgc tcaattttat cctgatgttt cgagaaacgg cgtaacagct
    37501 tgccgccaaa ccgccgcccg cacagataga gcacctggtc gccaatcatg ccaccaagcg
    37561 ccacagaaag taccaccagt gggaacttta atagcccctg atgtgccgca acgcctccca
    37621 gcaaagtcac ggtttcacct tccgccaggc taccgataac cagcgcggca taaccatatt
    37681 gtgagatgag tgtattgaga gccataggcc agtttgtctc cctttggttg cctgtaagca
    37741 tacaccctgt gaatcattca cgctgaaaaa ggacatacat cagacggtgc aaattacatg
    37801 gatgaacaaa aaataatcac aagctggctt atacttgaac tgtcaggttc atgaactcac
    37861 aacaaggggg cggtatgagc catgtctggg gactgttttc ccatcccgat cgtgaaatgc
    37921 aggtgattaa tcgcgaaaac gaaacgattt ctcaccatta tacccaccac gttttgctga
    37981 tggcggcgat tccggtgatt tgcgccttca ttggcactac acagattggc tggaattttg
    38041 gcgatggcac tatcctgaag ttatcctggt ttaccggact ggcgctggct gtcttgtttt
    38101 acggcgttat gctggctggg gttgcggtga tggggcgggt catctggtgg atggcgcgca
    38161 attacccgca gcgtccgtct cttgcgcact gcatggtctt cgcgggctac gttgcaactc
    38221 cgctgttttt aagcggtctg gtggcgcttt atccactggt ctggttgtgt gcgctggtcg
    38281 gcacggtggc gctgttttac accggctacc tgctttacct tggtattccg agcttcctga
    38341 atatcaacaa agaggaaggc cttagcttct ccagttcgac gctggcgatt ggcgtgctgg
    38401 tactggaagt gctgctcacc ctaaccgtta tactatgggg ctacggttat cgtttgttct
    38461 agaaagttct ttgacgttgc attgctggcg taaatgtcag caatgcagca tttactcacg
    38521 attctccttt gacgatcgca ctttttgctc gttatgatgg cgtcgctagc ctcagtaaat
    38581 ccttactccg gcggtgcgca acccgtgcgc gtgaaccact atctgaatgc tcatcatgcc
    38641 gaaatttcga gtttctttat ttagcttggc cctgatgctg gctgtgcctt ttgcaccgca
    38701 ggccgttgcg aaaacggcag ccgctaccac cgcttcacaa ccggaaattg cctccggtag
    38761 cgcgatgatt gttgatctga ataccaacaa agtgatctat tcgaaccacc cggatctggt
    38821 gcgtccgatt gcgtctatca gcaaattaat gaccgcgatg gttgtgctgg atgcacgact
    38881 gccgctggat gaaaaactaa aagtggatat cagccagacg ccggagatga aaggggtcta
    38941 ttcgcgcgta cgactgaata gcgaaatcag ccgtaaagat atgctgttgc tggcgctgat
    39001 gtcttcagaa aaccgcgcgg cggcaagcct tgcgcaccat tatcccggtg gttacaaagc
    39061 ctttattaag gcaatgaatg cgaaagcgaa atcgctcgga atgaacaaca cgcgctttgt
    39121 tgaacctacc ggattgtcgg tgcataacgt ttcaactgcc cgtgacttaa ccaaactgct
    39181 cattgccagc aaacaatatc cgttgatcgg gcagttaagt actacccggg aagatatggc
    39241 aaccttctct aacccgacgt atacgctgcc gttccgcaat actaatcatc tggtgtatcg
    39301 cgataactgg aatattcagt taaccaaaac cggctttacc aatgcggcgg gccattgtct
    39361 ggtgatgcgt acggttatca ataataaacc ggtggcgctg gtagtgatgg acgcgtttgg
    39421 caaatatacc cattttgccg atgccagccg cctgcgtacc tggattgaaa ccggtaaagt
    39481 gatgcctgtg ccggcagcag cgttgagcta taaaaaacaa aaagccgccc aaatggcggc
    39541 ggcggggcag acggcacaga acgattaagt gaattcggat ggcgatactc tgccatccgt
    39601 aatttttact ccacttcctg ccagtttttc cgtttactgg ttttaccgat ccccggattc
    39661 atgctattgg tcggatcatt ctcgcgatag aacttctgca acgtctccgg tgctttatac
    39721 aaatgaccga cgttatgctc ggcagggtac tgcgcgccgc gctgctgtag cagttccagc
    39781 atctgttctt ttaacgcatg cacatccacg ccttttttca ctatgtaatc ctgatggaag
    39841 acatagcaca taaaatggcc gtaatagagc ttgtgcacca gctggctgtc gatctccggc
    39901 ggtaaatgct cataccactc ggtgtcgtta cgccgcagag cgatatccag tgccagaatg
    39961 tcttcgactt catcggaatg caccgcctga taacgaattg ctgcgcccgc agcggcgaaa
    40021 cggtgtaaaa acgctttgct accttcctcc ggcgtacaga caaagaaatc gccctcggcc
    40081 tgtttgaaat aatccaccag ccacgatttg gcttcaccga cgccatcgcc tgccattttt
    40141 aacagcagat gatgctcgta tttatcgcgc cagtttttca tgcgcggcgg taaatggctg
    40201 gggaacaggt gaccgaattt ttgcattgca cggtcagtaa aatgcggacg gaagaatttc
    40261 actttctcca gcatcgcatc ggtgcgtccc ttgagattaa agaagaacgg catcttgtcg
    40321 gtgcctaact tatcaatcat caggaaggtg tctttgccgt atttttccgc aatatcgtag
    40381 atatcccggt gcatatattc cccggcaacc ggcagatttt cgaagttagc cagaatatga
    40441 cggcggattt cggtcagcac ttccggctgg ttggtgccga tataaaacac ctgctgattt
    40501 ttttccgctt cgaaggtatc aagacgtacc gcaaagacgg ccagcttccc ggcgcaacca
    40561 gaagattcaa ataaccgatc cggatcggcg ttataacgtg cgggcgtgtc ggcttcaata
    40621 tcacgaacgc ggtggacata gtcataatcg tgggcgtgac gaccatcgtg acgcacatcg
    40681 tcatctttga tgcgatcgtc atccagcttg ctaaggatct gctccggcgt ttcgcccaga
    40741 tcaatcccca gatggttcac cagcgtcagt ttgccatctt catctatacg cgcgaataac
    40801 gacatttcgg tatacgccgg gccacgttgc accagcgagc cgccggagtt gttacaaata
    40861 ccgccgatga ccgatgcgcc tatacacgat gatccaatca ccgaatgcgg ttcgcgtccc
    40921 agcggtttga gggctttttc cagcgaatag agcgtggtgc ccggataggc cagcacctgt
    40981 tcgcccttgc cgagaacgtg cagcttgtcg agacgcaggg tgctgatgat cacgatatcg
    41041 cgatcatagt cgttaccgtt tggcgtcgat ccttcggtca ggcctgtatt ggcagcctgc
    41101 atcagaataa ttttgtcggc ggtgacgcag gctttcagca cccgccacaa ttccagtagt
    41161 gagccaggga aaacgacagc cagcgcgtcg ccctgaccag aacggaagcc cttgcgatag
    41221 cgggccgttt ttgcgggatc ggtgagcagg tgtgaatgac cgaccagacg ggcaagttca
    41281 ttcaaaaagg ctttattatc agttgttgtc atggaagaca ttttccactc cttgtggtgg
    41341 cgaaaaaata tcacaaacta gaatagcgcc ttgtatgaca attcagcgag tgaatatttt
    41401 gaaaattcag acaataaact gagcattctt tttctctctc catcatgctt atggcaaact
    41461 acggtttttg caccattttc gctgccacgt cgggcaacaa aggaagaaaa atccatatga
    41521 aatggctatg ttcagtagga atcgcggtga gtctggtcct gcagccagca ctggcggatg
    41581 atttattcgg taaccatcca ttaacgcctg aagcgcggga tgcgttcgtc accgatttgc
    41641 tcaagaaaat gacggttgat gagaaaattg gtcagctgcg cttaatcagc gtaggcccgg
    41701 ataatccgaa agaggcgatc cgcgagatga tcaaagacgg tcaggttggg gcgattttca
    41761 acaccgtaac ccgtcaggat atccgcgcca tgcaggatca ggtgatggaa ttaagccgcc
    41821 tgaaaattcc tcttttcttt gcttacgacg tgctgcacgg tcagcgcacg gtgttcccga
    41881 ttagcctcgg tctggcctcg tcttttaacc tcgatgcggt gaaaacggtc gggcgtgtct
    41941 ctgcttatga agcggcagat gatggcctga atatgacctg ggcaccgatg gtcgatgtct
    42001 cgcgcgatcc gcgctgggga cgtgcttccg aaggttttgg cgaagatacg tatctcacct
    42061 caataatggg taaaaccatg gtggaagcga tgcagggtaa aagcccggca gatcgttact
    42121 cggtgatgac cagcgtcaaa cactttgccg catacggcgc ggtagaaggc ggtaaagagt
    42181 acaacaccgt cgatatgagt ccgcagcgcc tgtttaatga ttatatgccg ccgtacaaag
    42241 cggggctgga cgcaggcagc ggcgcggtga tggtggcgct gaactcgctg aacggcacgc
    42301 cagccacctc cgattcctgg ctgctgaaag atgttctgcg cgaccagtgg ggctttaaag
    42361 gcatcaccgt ttccgatcac ggtgcaatca aagagctgat taaacatggc acggcggcag
    42421 acccggaaga tgcggtgcgc gtggcgctga aatccggaat caacatgagt atgagcgacg
    42481 agtactactc gaagtatctg cctgggttga ttaaatccgg caaagtgacc atggcagagc
    42541 tggacgatgc tgcccgccat gtactgaacg ttaaatatga tatggggttg tttaacgacc
    42601 catacagcca tttggggccg aaagagtctg acccggtgga taccaatgcc gaaagccgcc
    42661 tgcaccgtaa agaagcgcgt gaagtggcgc gcgaaagcct ggtgttgctg aaaaaccgtc
    42721 tcgaaacgtt accgctgaaa aaatcggcca ccattgcggt ggttgggcca ctggcggaca
    42781 gtaaacgtga cgtgatgggc agctggtccg cagccggtgt tgccgatcaa tccgtgaccg
    42841 tactgaccgg gattaaaaat tccgtcggtg aaaacggtaa agtgctgtat gccaaagggg
    42901 cgaacgttac cagtgacaaa ggcattatcg atttcctgaa tcagtatgaa gaagcggtca
    42961 aagtcgatcc gcgttcgccg caagagatga ttgatgaagc ggtgcagacg gcgaaacaat
    43021 ctgatgtggt ggtggctgta gtcggtgaag cacaggggat ggcgcacgaa gcctccagcc
    43081 ggaccgatat cactattccg caaggccaac gtgacttgat tgcggcgctg aaagccaccg
    43141 gtaaaccgct ggtgctggtg ctgatgaacg ggcgtccgct ggcgctggtg aaagaagatc
    43201 agcaggctga tgcgattctg gaaacctggt ttgcggggac tgaaggcggt aatgcaattg
    43261 ccgatgtatt gtttggcgat tacaacccgt ccggcaagct gccaatttcc ttcccgcgtt
    43321 ctgtcgggca gatcccggtg tactacagcc atctgaacac tggtcgtccg tataatgccg
    43381 acaagccgaa caaatacact tcgcgttatt ttgatgaagc taacggagcg ctttatccgt
    43441 tcggctatgg tctgagctat accactttca ccgtctctga tgtgaaactt tctgcgccga
    43501 ccatgaagcg tgacggcaaa gtgacggcca gcgtgcaggt gacgaacacc ggtaagcgcg
    43561 agggggccac ggtagtgcag atgtacttgc aggatgtgac ggcttccatg agtcgccctg
    43621 tgaaacagct gaaaggcttt gag
//
LOCUS       DAJTUN010000020        40802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTUN010000020 DAJTUN010000000
VERSION     DAJTUN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.020"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="OO470_001043"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="OO470_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6250367.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OO470_001044"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OO470_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6250368.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OO470_001045"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OO470_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6250369.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="OO470_001046"
     CDS             complement(3100..6843)
                     /locus_tag="OO470_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6250370.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OO470_001047"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OO470_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6250371.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="OO470_001048"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="OO470_001048"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6250372.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="OO470_001049"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="OO470_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6250373.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OO470_001050"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OO470_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6250374.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="OO470_001051"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="OO470_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6250375.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OO470_001052"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OO470_001052"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6250376.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OO470_001053"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OO470_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6250377.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="OO470_001054"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="OO470_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6250378.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="OO470_001055"
     CDS             16240..16347
                     /locus_tag="OO470_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6250379.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OO470_001056"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OO470_001056"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6250380.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="OO470_001057"
     CDS             complement(17386..18195)
                     /locus_tag="OO470_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6250381.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="OO470_001058"
     CDS             complement(18199..18591)
                     /locus_tag="OO470_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6250382.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OO470_001059"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OO470_001059"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6250383.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OO470_001060"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OO470_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6250384.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OO470_001061"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OO470_001061"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6250385.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="OO470_001062"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="OO470_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6250386.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="OO470_001063"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="OO470_001063"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6250387.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /gene="prs"
                     /locus_tag="OO470_001064"
     CDS             23640..24587
                     /gene="prs"
                     /locus_tag="OO470_001064"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6250388.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="OO470_001065"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="OO470_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6250389.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OO470_001066"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OO470_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6250390.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="OO470_001067"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="OO470_001067"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6250391.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="OO470_001068"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="OO470_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6250392.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29637..32504
                     /locus_tag="OO470_001069"
     CDS             29637..32504
                     /locus_tag="OO470_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6250393.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OO470_001070"
     CDS             complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OO470_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6250394.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            34751..35821
                     /gene="dhaK"
                     /locus_tag="OO470_001071"
     CDS             34751..35821
                     /gene="dhaK"
                     /locus_tag="OO470_001071"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6250395.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35832..36464
                     /gene="dhaL"
                     /locus_tag="OO470_001072"
     CDS             35832..36464
                     /gene="dhaL"
                     /locus_tag="OO470_001072"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6250396.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36475..37893
                     /gene="dhaM"
                     /locus_tag="OO470_001073"
     CDS             36475..37893
                     /gene="dhaM"
                     /locus_tag="OO470_001073"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6250397.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38213..39910
                     /gene="treA"
                     /locus_tag="OO470_001074"
     CDS             38213..39910
                     /gene="treA"
                     /locus_tag="OO470_001074"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6250398.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGKKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(39989..40428)
                     /locus_tag="OO470_001075"
                     /pseudo
     CDS             complement(39989..40428)
                     /locus_tag="OO470_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10087 a        10575 c        10175 g         9965 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactgga cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgctagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaagt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccaa gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atagccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccaag taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgccggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg ctatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccctggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtga ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt cctgcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacggaacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gtgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgactttct ggcacgacct ggcagcaccg atcctggcgg ggattattgc cgcagcgatt
    16321 gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 agcggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatccgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgcctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcgacaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttacca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgcacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cctggaaagg atcatgcctg
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg ccccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctattctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct ggagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatagcc
    28321 aacgtcaacg aagatggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatctt ggcaagatga
    29161 aaatgataac cagtagcaca ataataacga taggccagaa aatccattga acaaaaatga
    29221 atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt
    29281 tatgcagata catcaagcga aaatcccata actaattagg attattacaa aaaatcttca
    29341 ccaaattttt taaccgctgt ttgttgtaat atttttgcca tattcatagt aacgaattgg
    29401 aataagtacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    29461 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    29521 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    29581 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    29641 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    29701 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    29761 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    29821 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    29881 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    29941 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    30001 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    30061 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    30121 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    30181 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    30241 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    30301 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    30361 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    30421 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    30481 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    30541 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt tcagacgcga
    30601 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    30661 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    30721 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    30781 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    30841 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    30901 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    30961 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    31021 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    31081 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    31141 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    31201 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    31261 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    31321 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    31381 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    31441 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    31501 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    31561 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    31621 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    31681 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    31741 gcctggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    31801 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    31861 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    31921 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    31981 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    32041 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    32101 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    32161 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    32221 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    32281 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    32341 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    32401 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    32461 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    32521 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    32581 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    32641 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    32701 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    32761 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    32821 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    32881 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    32941 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    33001 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    33061 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    33121 aggtccacgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    33181 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    33241 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    33301 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    33361 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    33421 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    33481 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    33541 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    33601 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    33661 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    33721 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    33781 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    33841 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    33901 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    33961 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    34021 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    34081 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    34141 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    34201 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    34261 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    34321 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    34381 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    34441 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    34501 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    34561 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    34621 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    34681 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    34741 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    34801 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    34861 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    34921 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    34981 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    35041 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    35101 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    35161 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacacag tattaattga
    35221 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gaggcctgtg cggaactggg
    35281 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    35341 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtcggcat
    35401 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    35461 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    35521 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    35581 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    35641 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    35701 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    35761 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    35821 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    35881 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    35941 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    36001 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    36061 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    36121 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    36181 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    36241 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    36301 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    36361 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    36421 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    36481 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    36541 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    36601 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    36661 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    36721 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    36781 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    36841 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    36901 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    36961 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    37021 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    37081 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    37141 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    37201 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    37261 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    37321 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    37381 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    37441 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    37501 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    37561 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    37621 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    37681 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    37741 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    37801 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    37861 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    37921 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    37981 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    38041 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    38101 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    38161 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    38221 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    38281 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    38341 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
    38401 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    38461 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    38521 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    38581 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    38641 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt
    38701 caccatgtta ggacttgccg aaagtggtca ctgggataaa gtcgcggata tggtggccaa
    38761 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    38821 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    38881 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    38941 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
    39001 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
    39061 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    39121 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    39181 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    39241 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    39301 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    39361 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    39421 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    39481 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    39541 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    39601 attacaaaac tacgggaaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    39661 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    39721 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    39781 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    39841 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    39901 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    39961 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    40021 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    40081 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    40141 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    40201 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    40261 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    40321 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    40381 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    40441 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    40501 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    40561 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    40621 ttcctgatgc gtttggttct ctgcggattg tcgctggcga aataaatggc ttgcggtaaa
    40681 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccggctt ttggcgagag
    40741 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    40801 at
//
LOCUS       DAJTUN010000021        40164 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTUN010000021 DAJTUN010000000
VERSION     DAJTUN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.021"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            81..1514
                     /gene="cscA"
                     /locus_tag="OO470_001076"
     CDS             81..1514
                     /gene="cscA"
                     /locus_tag="OO470_001076"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6250399.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1522..2580)
                     /locus_tag="OO470_001077"
     CDS             complement(1522..2580)
                     /locus_tag="OO470_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6250400.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2748..2966
                     /locus_tag="OO470_001078"
                     /pseudo
     CDS             2748..2966
                     /locus_tag="OO470_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2984..4312
                     /gene="dsdA"
                     /locus_tag="OO470_001079"
     CDS             2984..4312
                     /gene="dsdA"
                     /locus_tag="OO470_001079"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6250401.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4420..5958)
                     /gene="emrY"
                     /locus_tag="OO470_001080"
     CDS             complement(4420..5958)
                     /gene="emrY"
                     /locus_tag="OO470_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6250402.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5958..7121)
                     /gene="emrK"
                     /locus_tag="OO470_001081"
     CDS             complement(5958..7121)
                     /gene="emrK"
                     /locus_tag="OO470_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6250403.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7537..8151
                     /gene="evgA"
                     /locus_tag="OO470_001082"
     CDS             7537..8151
                     /gene="evgA"
                     /locus_tag="OO470_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6250404.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8156..11749
                     /gene="evgS"
                     /locus_tag="OO470_001083"
     CDS             8156..11749
                     /gene="evgS"
                     /locus_tag="OO470_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6250405.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11805..12950)
                     /gene="yfdE"
                     /locus_tag="OO470_001084"
     CDS             complement(11805..12950)
                     /gene="yfdE"
                     /locus_tag="OO470_001084"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6250406.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13024..13968)
                     /gene="yfdV"
                     /locus_tag="OO470_001085"
     CDS             complement(13024..13968)
                     /gene="yfdV"
                     /locus_tag="OO470_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6250407.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14038..15732)
                     /gene="oxc"
                     /locus_tag="OO470_001086"
     CDS             complement(14038..15732)
                     /gene="oxc"
                     /locus_tag="OO470_001086"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6250408.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15786..17036)
                     /gene="frc"
                     /locus_tag="OO470_001087"
     CDS             complement(15786..17036)
                     /gene="frc"
                     /locus_tag="OO470_001087"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6250409.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17549..18172)
                     /locus_tag="OO470_001088"
     CDS             complement(17549..18172)
                     /locus_tag="OO470_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6250410.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18469..18744
                     /locus_tag="OO470_001089"
     CDS             18469..18744
                     /locus_tag="OO470_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250411.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18821..19063)
                     /locus_tag="OO470_001090"
     CDS             complement(18821..19063)
                     /locus_tag="OO470_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6250412.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19416..20336
                     /gene="lpxP"
                     /locus_tag="OO470_001091"
     CDS             19416..20336
                     /gene="lpxP"
                     /locus_tag="OO470_001091"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6250413.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20690..20761
                     /gene="ypdK"
                     /locus_tag="OO470_001092"
     CDS             20690..20761
                     /gene="ypdK"
                     /locus_tag="OO470_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6250414.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20826..22064)
                     /gene="alaC"
                     /locus_tag="OO470_001093"
     CDS             complement(20826..22064)
                     /gene="alaC"
                     /locus_tag="OO470_001093"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6250415.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22441..23927
                     /locus_tag="OO470_001094"
                     /pseudo
     CDS             22441..23927
                     /locus_tag="OO470_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23942..24676
                     /gene="ypdB"
                     /locus_tag="OO470_001095"
     CDS             23942..24676
                     /gene="ypdB"
                     /locus_tag="OO470_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6250416.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24689..25546
                     /locus_tag="OO470_001096"
     CDS             24689..25546
                     /locus_tag="OO470_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6250417.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25549..28044)
                     /gene="ptsP"
                     /locus_tag="OO470_001097"
     CDS             complement(25549..28044)
                     /gene="ptsP"
                     /locus_tag="OO470_001097"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6250418.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28069..29106)
                     /gene="ypdE"
                     /locus_tag="OO470_001098"
     CDS             complement(28069..29106)
                     /gene="ypdE"
                     /locus_tag="OO470_001098"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6250419.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29106..30189)
                     /gene="ypdF"
                     /locus_tag="OO470_001099"
                     /pseudo
     CDS             complement(29106..30189)
                     /gene="ypdF"
                     /locus_tag="OO470_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30204..31451)
                     /locus_tag="OO470_001100"
     CDS             complement(30204..31451)
                     /locus_tag="OO470_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6250420.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31473..31799)
                     /locus_tag="OO470_001101"
     CDS             complement(31473..31799)
                     /locus_tag="OO470_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6250421.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32018..32983)
                     /gene="glk"
                     /locus_tag="OO470_001102"
     CDS             complement(32018..32983)
                     /gene="glk"
                     /locus_tag="OO470_001102"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6250422.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33187..34443
                     /locus_tag="OO470_001103"
     CDS             33187..34443
                     /locus_tag="OO470_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6250423.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34558..34884
                     /locus_tag="OO470_001104"
     CDS             34558..34884
                     /locus_tag="OO470_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6250424.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35025..36263)
                     /locus_tag="OO470_001105"
     CDS             complement(35025..36263)
                     /locus_tag="OO470_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6250425.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36599..37801
                     /gene="nupC"
                     /locus_tag="OO470_001106"
     CDS             36599..37801
                     /gene="nupC"
                     /locus_tag="OO470_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6250426.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37851..39963)
                     /locus_tag="OO470_001107"
                     /pseudo
     CDS             complement(37851..39963)
                     /locus_tag="OO470_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10706 a         9993 c         9294 g        10171 t
ORIGIN      
        1 atttatgaaa gtaagggact atatttttat aaaagttaac gttaataatt caccaaattt
       61 gcttaaccag gatgattaaa atgacgcaat ctcgattgca tgcggcgcaa aacgcactag
      121 caaaacttca tgagcaccgg ggtaacactt tctatcccca ttttcacctc gcgcctcctg
      181 ccggatggat gaacgatcca aacggcctga tctggtttaa cgatcgttat cacgcgtttt
      241 atcaacatca cccgatgagc gaacactggg gaccaatgca ctggggacat gccaccagtg
      301 acgatatgat ccactggcag catgagccta ttgcgctagc gccaggagac gataatgaca
      361 aagacgggtg tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca
      421 ccggacacgt ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc
      481 tggctaccag tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag
      541 aaggaatcat gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg
      601 tagtcggggc gaaagatcca ggcaacacgg ggcagatcct gctttatcgc ggcagttcat
      661 tgcgtgaatg ggccttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt
      721 gggaatgtcc ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg
      781 gaatgaatgc cgagggatac agttaccgaa atcgctttca aagtggcgta atacccggaa
      841 tgtggtcgcc aggacgactt tttgcacaat ccgggcattt tactgaactt gataacgggc
      901 atgactttta tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct
      961 ggatggatat gtgggaatcg ccaatgccct caaaacgtga aggatgggca ggctgcatga
     1021 cgctggcgcg cgagctatca gagagcaatg gcaaacttct acaacgcccg gttcacgaag
     1081 ctgagtcgtt acgccagcag catcaatctg tctctccccg cagaatcagc aataaatatg
     1141 ttttgcagga aaacgcgcaa gcagttgaga ttcagttgca gtgggcgctg aagaacagtg
     1201 atgccgaaca ttacggatta cagctcggca ctggaatgcg gctgtatatt gataaccaat
     1261 ctgagcgact tattttgtgg cggtattacc cacacgagaa tttagacggc taccgtagta
     1321 ttcccctccc gcagcgtgac acgctcgccc taaggatatt tatcgataca tcatccgtgg
     1381 aagtatttat taacgacggg gaagcggtga tgagtagtcg aatctatccg cagccagaag
     1441 aacgggaact gtcgctttat gcctcccacg gagtggctgt gctgcaacat ggagcactct
     1501 ggctactggg ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg
     1561 cgtcactctt cccccttcac gaccttcaat aatatgcaat gcagcttccc gcccgataat
     1621 gtcatgtgga agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag
     1681 attatcaaag cccattacag cgacatcctg cgggatacgt acccccttcg ccaaaagaac
     1741 ctgataagcc acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt
     1801 gcccgatttg aagtgagcat tgagtaaact tgcgagatcg gtgtagtgat catcacctgt
     1861 tgccatgtga aattgtttca cctcagccag atctcgtcca gcatcacgcc aagcctgctc
     1921 aaatccctgc cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataatgg
     1981 ttgacgatag cccgccgcga gcaaatgctg tgttgattca tattgtgcag tgtaatcatc
     2041 agggatataa ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc
     2101 accatacaga gactcaggca gcgtgatatg tcgcagcccc attgtagtat agataatgcc
     2161 atccggacgg tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat
     2221 attgattaaa aaactattcc agccgaactc gctggcggtt tgctcaatgg caagcagaat
     2281 atcaacagag aaaggagtgg tagccgtgtc ctgcgccagc acggcgagag tcgacggctt
     2341 acgtccttga gcgcgcatct tacgggcgga aagatcagga acataattca gggtctggat
     2401 tgcctgcaat acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg
     2461 ggagactgtc atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc
     2521 caagccgttt gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa
     2581 cgccctatca cctgacacag caatacaata aaaaataaca ataattcccg gacaattgtc
     2641 cccaattccg cctctgttct cgccaacgag taaccatata ggcaagtaag taaaacatgc
     2701 caactggcat cgccaacctg gagaagaaaa tataaatatg gatcattgtg ctgccactgt
     2761 atcccgacat cagcccggaa attattgcga ttgctatcgg ttcaggtgca attggctgca
     2821 cgatcgttac ggactcgctt ttctggctgg tgaagcaata ttgcggcgct acgctcaatg
     2881 aaacatttaa atactatacg acagcgacat ttatcgcttc agtcgtcgct ctggcgggca
     2941 cattcctgct gtcatttatc atctaagcgc aaagagacgt actatggaaa acgctaaaat
     3001 gaactcgctc atcgcccagt atccgttggt aaaggatctg gttgctctta aagaaaccac
     3061 ctggtttaat cctagcacga cctcattggc tgaaggttta ccttatgttg gcctgaccga
     3121 acaggatgtt caggacgccc atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc
     3181 atttgctgaa acagctgcca ctggggggat tattgaatca gaactggttg ccattccggc
     3241 tatgcaaaaa cggctggaaa aggaatatca acaaccgatc agcgggcaac tcctactgaa
     3301 aaaagatagc catttgccca tttccggctc tataaaagca cgcggcggga tttatgaagt
     3361 cctggcacat gcagaaaaac tggctctgga agcggggttg ctgacgcttg atgatgacta
     3421 cagcaaactg ctttctccgg agtttaaaca gttctttagc caatacagca ttgctgtggg
     3481 ctcaaccgga aatctggggt tatcaatcgg cattatgagc gcccgcattg gctttaaggt
     3541 gacagttcat atgtctgctg atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg
     3601 cgttacggtc gtggaatatg agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc
     3661 agcgcagtct gacccgaact gtttctttat tgatgacgaa aattcccgca cgttgttcct
     3721 tgggtattcc gttgctggcc agcgtcttaa agcgcaattt gcccagcaag gccgtatcgt
     3781 cgatgctgat aaccctctgt ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg
     3841 cgtcgcattc gggcttaaac tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc
     3901 aacgcactcc ccttgtatgt tgttaggcgt ccatacagga ttacacgatc agatttctgt
     3961 tcaggatatt ggtatcgaca accttaccgc agcggatggc cttgcagttg gtcgcgcatc
     4021 atgctttgtc gggcgggcaa tggagcgtct gctggatggc ttctataccc ttagcgatca
     4081 aaccatgtat gacatgcttg gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc
     4141 agcactggcg ggtatggccg gacctcagcg cgtgtgtgca tcagtaagtt accaacagat
     4201 gcacggtttc agcgcagaac aattgcgtaa tgccactcat ctggtgtggg cgacgggagg
     4261 tggaatggtg ccggaagaag agatgaatca atatctggca aaaggccgtt aataacgttt
     4321 caacgcaaca tcgcaatcct ttccctgggt gagcgatgct gccgatggcg cagacttaag
     4381 atccccggtc ttacccgcta taaccccctc cttttatcat cacccaacgc ctttcgctgt
     4441 aaacggcggt ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat
     4501 acggaaaatc tcatttgcag aaatagaaag tgactgctgg gttatttcat tattaatttc
     4561 attaagaact cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa
     4621 cacggggtta aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcgtcg
     4681 tccccacagc gtcattgtca acgacgtacc aactgatcct gacaaggtac gaaaaaaatt
     4741 actcatactc gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt
     4801 taagggtaaa aagaaacagg caacggcgaa tccctggaaa aactgtggca aaatgatgcc
     4861 tgtaaaatca atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat
     4921 caaaaaactg aatgtcacta acacccgcat gtctatttta ttgccataac gtcctatcaa
     4981 aggtgaaatc aatagtggca tgatgccgat gggcgcataa gcaagcccgg cccatatcgc
     5041 attatacccc atcgttttct ggagtaactg cggcataagg acgatcgctc cagagtaaaa
     5101 taaatacgcg caggtgatac tcacaatacc aatggtgaaa ttacgggact taaacaaact
     5161 gagatcaaga atcgggttct ctgaggtcga ttcccaaatg actaaagaga tcagagaaat
     5221 aactgatact actgttaata ttattattgt actcgagttg aaccaatcca gatcgcgccc
     5281 tttatcaagc ataatttgca agccaccaac accgagcact aacagggtca gtcttggtag
     5341 attcattttt accggtgaag tttcagtttc tcttccttta agtaaggtta agcataatgt
     5401 caggacgata atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc
     5461 gcaaatatag ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca
     5521 taatgccaga gcaaatgttc ttttttctgg tggataattc cttaataata aactctgtga
     5581 cagtggaatt aacggccccg ccattaatcc ctgaacgact ctaaaaaata tcagcacatc
     5641 aagattggtc gataaactac acattaagga agacagcgaa aaaaaagtga ctgaaagtaa
     5701 aaataacctt aattcgccta ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc
     5761 atttgctacg ccaaacgagg tgataaccca ggtgccttcg tctgttgatg ctcccagaaa
     5821 gccagatatt gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaacgt
     5881 cgctaatgac aatgcaatag taacgcacca taacgtccca ccggttaatg gtgccggagt
     5941 tgatttagtg attgccatta aagttgtcca ttatgcgaaa taatgttgct aatttctttt
     6001 tctatcggac tggtatcgat aactaaagcc ttactggtat aagcaggcat ggaggtcacg
     6061 gttgaagcca gatcaggcat ctcagcaatg tcttcgtcct tcgtatcaat agttgcagtc
     6121 atcgataaac caatgcgcaa ggggtgttcc atgagttctt ttggatcaag agaaacttca
     6181 accggtacac gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag
     6241 gagaacgcat tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt
     6301 tcaccataaa gatcgctgat aatattgact gattgaccaa tccgtacatc cgtgagttgt
     6361 gtttctttaa agttggcatt aacccacatt tgacgtgccg gtacgacagc cattaacgat
     6421 tgtccggaac tcactgtttc gccgacctga acacttcgct gggcaatata gccagtaacc
     6481 ggactcctta tatccgtacg tttaagcacc aaccaggctt ctttggttgc atccgccgct
     6541 tcaactactt gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta
     6601 taagcctgga tagcggcatt cagtgccgct ttgctactta ttaacgtatc tttggtatgc
     6661 tccagcgttt cttttgaaat aaccccctgc ttcgctaaag gcacacgacg gttataatct
     6721 tctaaagatt gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc
     6781 tgtaagtaca gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg
     6841 agtgcgatag tggcatcagt tttatccagt gaaactaaaa tgtcaccctg tcgaacgtag
     6901 ttcgtatctt tatgattaac tacagtgaca ctacctgaga cttgtgcaga aattggatct
     6961 gcattccccg tgacataggc gtcatctgta ctaatcatgt cttctaattc cattgaccaa
     7021 taggcatagg cacctgaaaa cgcaataaat aaaactatca ccaataaaga aaagtatttt
     7081 tttctgttag actgtttttt atttgaatta atctgttcca ctattatctc tcatttcgca
     7141 tagatgaaat ttatgaattg aatgaaaaga gtattcattc ttcatatcaa acacagtgca
     7201 tgtgttgcac attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac
     7261 aacagaggac taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtgaga
     7321 agacttatag tgctaacttg aaactataaa tcatcggtat aatccctgat tttattgttg
     7381 acatttcatt tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaaact
     7441 cagattttct tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg
     7501 gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac
     7561 catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta
     7621 gcggagttaa ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa gcctgatata
     7681 gtcatcattg atgtcgatat ccccggagtt aacggtatcc aagtgttaga aacgctgagg
     7741 aagcgccaat atagcggaat tattattatc gtctccgcta aaaatgacca tttttacggg
     7801 aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat
     7861 atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt ctctctcaac
     7921 cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa
     7981 attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc ggaaaaaatg
     8041 ttcatcagca acaaaactgt cagcacgtat aaaagtcgcc tgatggaaaa attagaatgt
     8101 aaatcactga tggatcttta cacattcgcg caacgtaaca aaatcggcta atcacatgaa
     8161 gtttttaccc tatttttttc ttctctgttg tggtctttgg tcgaccataa gtttcgcaga
     8221 cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccagt
     8281 acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt
     8341 acacaagtcc caaacggcta ccttgttgca taccgattcg cagcaacagg ttcgtggtat
     8401 taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa cactccggga
     8461 atacgcagat catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt
     8521 atcacattta gttacttcgc cgcctcttaa taatgacatt gctgcaacca aaccattgat
     8581 aattaccttt ccggcgctgg taaccaccct tcacgactca atgcgaccgc ttacctcacc
     8641 aaaaccagta aatattgctc gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc
     8701 atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc
     8761 agctgggcac aatgattact ttattggtag taacatcatt accagcagta tgatttcccg
     8821 ctatttcact cactccttaa atgtagtgaa atattataac tcgccgcgtc aatataattt
     8881 tttcttgacc agaaaagaat ctgtcattct taatgaagta ctcaatagat ttgttgatgc
     8941 tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc
     9001 ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc
     9061 caatttaaag gtgctggaaa atccttactc gcccccctat tctatgacgg atgaaaatgg
     9121 ctcggttcgg ggcgttatgg gggacattct taatattatt accttgcaaa caggtttaaa
     9181 tttttctccg atcaccgttt cacacaatat ccatgctgga acacaactta gccccggagg
     9241 atgggatata atacctggcg ctatttatag tgaagatcga gaaaataatg ttttatttgc
     9301 tgaagccttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca
     9361 aacattaaaa aaaggaatga aagttgccat tccatattat tatgagctgc attcgcaatt
     9421 aaaagagatg tatccggagg ttgaatggat acaggtcgat aatgccagcg ctgcatttca
     9481 caaggttaag gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat
     9541 gatcgatcat tactatccta atgaacttta tcattttctt attcctggcg ttccgaatgc
     9601 atcgctttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc
     9661 actgaatgca attcccccaa gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc
     9721 caatgtgacc attgacacat gggacctata tagcgagcaa ttttatattg ttacgacatt
     9781 atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg
     9841 tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata tcattccgaa aagcactctc
     9901 ggattcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcataa
     9961 tagtgctttt gaacattatt tcactgcgga ttactacaaa aatgcaatgt taccattaga
    10021 aaacagtgat tcacccttta aagatgtttt ttctaatgcg catgaagtca cagcagaaac
    10081 gaaagaaaat cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag
    10141 atgcattaat cactggcata cattatgcaa tcttcctgca agtgacaatg cagtatatat
    10201 ttgtggttgg caagatatta ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa
    10261 aaataaagcg ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga
    10321 aataagaaca ccaataagct ctattatggg cttcctggaa cttctgtcgg gttctggtct
    10381 tagcaaggag caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct
    10441 cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca
    10501 accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat
    10561 tgctgcaagc aaatcgatcg cattaagttg cagcagtaca tttcctgatc attacctggt
    10621 taagatcgac cctcaggcgt ttaagcaggt cttatcaaat ttactgagta atgctctcaa
    10681 atttaccacc gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca
    10741 cgctgttatc aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca
    10801 acaactgttt aagcgctaca gccaaacaag tgcaggtcgt cagcaaacag gttctggttt
    10861 aggcttaatg atctgcaaag aattaattaa aaatatgcag ggcgatttat cattagaaag
    10921 tcatccaggc ataggaacaa catttacgat cacaatcccg gtagaaatta gccagcaagt
    10981 ggcgactgtc gaggcaaaag cagaacaacc catcacacta cctgaaaagt tgagcatatt
    11041 aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg
    11101 atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca
    11161 ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg
    11221 caaactccgt gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc
    11281 taacgaacgt gaaaaagggt taagttgcgg catgaactta tgtttgttca aaccgttgac
    11341 cctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca
    11401 gtatcgccac cttgatatcg aagccctgaa aaataatacg gcgaatgatc tacaactgat
    11461 gcaggagatt ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca
    11521 tgcactagaa gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc
    11581 ggctaacatc ctgaatttgc aaaagttgat taatattagc catcagttag aaataacacc
    11641 tgcttcagat gacagtaagc ctgaaattct tcagctgctg aactctgtta aagaacacat
    11701 tgcagagctg gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa ttgcggctcc
    11761 cacaatgttc taatgtggga gctatttacc agcacatctt acatttatga tgagaactcc
    11821 tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc
    11881 gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcctccggc ttcaatcaac
    11941 atatttctcg cctgagtttg tggcaaatta atggcctcag ccacacttaa taacggcgcg
    12001 acgggcacac caacttcatg tattttggct aaccaaactt cagctgcctg cgtttttaac
    12061 gttcgctcaa tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta
    12121 aatcggggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc
    12181 ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt
    12241 gccatgtagg gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc
    12301 atcagaccat gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc
    12361 cctctctggc tcttttcgcg gccataaagt gcactcacta ttccgctgaa taaatagaca
    12421 ccgccgcata gatccgcaag agaggtacca acacgcactg gcggagcatc agggtaccct
    12481 gtttccatca ttatcccact cattgcctga atgatggtat cgtaggcagg agcatctttt
    12541 agcggaccgg tatgtccgaa acctgacgat gaagcatata tgaggcgcgg gttgatttct
    12601 tgtaaccttt cccatgaaaa ccccagtttt tccattgtac ctaggcgaaa attctcagct
    12661 aatacatcag cttgtttgag catatttata aatatacttt tatcgtgatc attctttaaa
    12721 tcaagaacca cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc
    12781 acatagggac caaatgtgcg ggtatcatca ccatgacctg gtggctcaac tttaattacc
    12841 cttgcgccca tattataaag aagttgagtt ccgaaaggtc cattaaggac atgtgtcata
    12901 tcgataacta ataagccttc aaacggccct ttgctttcat tatttgtcat ttgcttatgc
    12961 cttgaaaaaa gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat
    13021 taattatgaa accagtcgac tgacataaat ccacaaggga gccgtgacga caaagcccag
    13081 tacgctcaca gccaatgacg ctgtaccagt acgggtgtag acattaaacc ggctggcaat
    13141 aatgattccg gagaatgccg gcggtaatgc gcctgccagt accatcatct gcaaatgttc
    13201 gctgttcaaa tgacatgcca taccaacgag aagcagcgcc agtggcatca gaatcaactt
    13261 caggaaggtg ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa
    13321 ccccgcagcg aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc
    13381 ccatgctgcc ggaattttta ccccaaccag caccaggatc gttgccagaa caggtgccca
    13441 tactactggc tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc
    13501 cgctcctgag gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat
    13561 aatagaaata attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag
    13621 aactgcaaac cccaggaatc caatggtagg tgaacccgca attaatgcac atacagctgc
    13681 ttctgcatgg gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa agaaacatcc
    13741 gacaataacc acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt
    13801 tgcccgggta atagaaacaa atagagccgc aggaagagcg tagtttaata ccagtttatt
    13861 aaatgcgcgg gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag
    13921 catgattacg ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc
    13981 gataagggtc ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta
    14041 attaccagcg acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc
    14101 agggtcgata accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc
    14161 atgacgaagt tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa
    14221 tttgtcatac cttgcatggt gcaacagatc cgttggtgat ggtgcaccag cgccactgag
    14281 atcaacaccg tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag
    14341 gttatatcga caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc
    14401 aatggcgaca accggagaac cagaagtcac gctagcaccg atggcatagc ccataccgat
    14461 gcccatgaca ccccaggtac cacaatccag acgacgacgt ggtttataca tatcaataat
    14521 atttcgtgca ttatccaggg tatttgcacc ttcattaact aaataaatat cctggttctc
    14581 gcgcaatacg tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt
    14641 acttaatttt tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg
    14701 ccataccagt ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact
    14761 ggatgcaata tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg
    14821 ttcaatatcc agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccaa
    14881 taaccaattc agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga
    14941 acgcgcagct gccgcagaaa gtggatgcgt atcttcaagg atccctttcg ccatagacat
    15001 tggcaggaat ggaatctgag cactttcaat aaattcacga agctgttcat cagcttgtga
    15061 atacgccgcg cctttgccaa ggataattaa tggccgttca gcttttgcta aaagcgaaat
    15121 tgcgctagtg actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac
    15181 aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc
    15241 aagataaact ccgccagggc gacccgatac agagacccgg atagcgcgtg ccaatgcaat
    15301 gccaagatcc tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt
    15361 catttggtcc agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga
    15421 gccgctaatc atgataatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc
    15481 attgaggaat cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc
    15541 gcttgccgca gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc
    15601 ttccgcctgg gcatggcgtg ccatatccgt cacaggaata cctacaacac cataaatagt
    15661 gtcaatatta ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag
    15721 ttgatctgac atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt
    15781 taatatcaga tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc
    15841 tcctgcaata cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta
    15901 aaggcagaga atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc
    15961 acttcaacaa cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt
    16021 aaaaccggtg cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca
    16081 atagtgacag tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca
    16141 tgggctgtac tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg
    16201 ttttcccagt tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag
    16261 cctttacatt tcaggatcca gccaggctga ccgccaccac ctgcattacc accgcgggga
    16321 actgcatcac caaatgtacc attcggatac tgcgggtatt cttccagata acccaattta
    16381 tcgagacgct gctggtcacg taatttcacg cggcaaaggt tcaatacggc atcctgcatt
    16441 gacatggtga ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa
    16501 ccgatcagca aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc
    16561 ggaccatccc aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc aacgttttca
    16621 taggctttta cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc
    16681 agacgtggat tgatttcttg aatatgctcc caggtgaagc ccatgtgatc aatggcccct
    16741 ggatgaaagt tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg
    16801 ccttccgctg ttttggtatt taactcaata gaacgtttgt tactgttaag catggtgaag
    16861 taaagcgcat cgatatcagg aatatctcgc agttggtgac gcgttacgtc accaacgccg
    16921 ggacgttcaa ttttaataac gtcagcgcca aaccaggcca gcatttgagt acaagatggg
    16981 ccagattgca caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca
    17041 atacctatta gttaattaaa tatgtgttaa tttagcgttt atttaaggca taacgccctc
    17101 tataaataat ctgcaaaata tcttcgtagc ggcactcgat atttttaaat tcctgtatca
    17161 agtactgaaa attattttat cgccacttcc aattcctttc tgcatggtaa gtagtgagga
    17221 cgaaaagaag aatatgataa atattgattc gtggtaatca gagagagtcg cgacatttgt
    17281 gatgttattc ttacaactgt aagaagcgcc ggtgatttac aagtaagagt atgattacgc
    17341 tttttaaagc gtaccataaa gcataacata ataaatgatt tgtgatgaca cgggattatt
    17401 gcggtttact ttgaggaaaa taaaatgcgt gtttatagaa ccgcgagcgc caaataattt
    17461 tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt cggctggagg gagttaaatc
    17521 ctttacctca atatactgcc tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga
    17581 tgattccatc ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa
    17641 gcagtttctg cgcatcagca agtgcattac gagtctgttt aagcggcatc agatattggt
    17701 tttccattac gccaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt
    17761 tcgccatttt ttcattggca atttttatcg cggcttcttt ttcgggtgtt gctacataac
    17821 tctcagaaat tccgacagat gcattaatga caatatattg gtcatcattg agattggtct
    17881 ttttacccgg cttagcgaac tttgcccatt cggtactgtc atctgacagc aaagcggaag
    17941 cttcattggt cagttctttt gccttttccg gatcgccatg aaacagagcc aggcgcgcta
    18001 cctgcacgtc acgcatcgca tataaacctt gttcagagat acgctctgct gctgccgttt
    18061 tctgggtttg ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag
    18121 ttgaagatgc cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct
    18181 cattctttaa cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat
    18241 cagagagcaa cggtcatttt gaaagggtta tcttacaatt gtagttatta tcgcaaagcg
    18301 gcatttagtc ttacagttgt aggaatatgc ctcctgtcgc agagaactat tcttatatac
    18361 cacaaagaga tgtcatttat tatattgatg taaatattga ttaattatat gtaaatacag
    18421 ccataacatc gtatggttct cagtgaacca ttttcaggat aactttctat gaaagtaaac
    18481 ttaatacttt tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt
    18541 tccatttctg gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg
    18601 acgaccacac actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa
    18661 gatgtgccac cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat
    18721 tatcttattc atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat
    18781 gctgatgtga acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca
    18841 ccataaagcg aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacca
    18901 gttcattacc gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca
    18961 tgccgaagaa agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta
    19021 cgcagacaat acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg
    19081 cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa
    19141 tttttgctaa gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat
    19201 tttttacttt gcacgattaa taatcagtag ctgaaagcag tcagccagtg ataaagtaga
    19261 gccaccgcat tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga
    19321 accattgtcg tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc
    19381 tgcgatacaa caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc
    19441 gcgagtttct acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt
    19501 gggtacagct tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc
    19561 gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc
    19621 agcattctgc ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg
    19681 cgctggtaga aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg
    19741 atgttgaagg gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg
    19801 tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa
    19861 tgatggctac ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc
    19921 gcatgcgctc taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac
    19981 tgaagaaagg tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct
    20041 ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct
    20101 cccgtctctc tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg
    20161 gatatcgttt gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg
    20221 ctgcttatat gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct
    20281 ggatccaccg tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa
    20341 ctgctcttac tctttccctc ctcaaatcgg ccatgataac ctcatgattt ttatagcttt
    20401 ttcttaagtt ttttccttcg ctgaatatct aattattacc gtcagaaatt aaactgtcgc
    20461 cgatatgaga cacttgtaag ttactaaaag taaataagaa aatgcctctt gtctcgtttc
    20521 agttttaggc gtacattagc gccgtctcga gcagtttagc tcagaattct ggggtgatgc
    20581 ccacggtaaa atacagggct tttaaggtcg ctttgcccgg tgtcaactca ctgtatcagg
    20641 tgtaatgaag tcattcaggc gtaacagtaa ttacgcggag agatgtaaag tgaaatattt
    20701 ctttatgggc atttctttta tggtcatcgt ttgggccggt acttttgccc tgatgatcta
    20761 aaagcaaact gtgcaatgaa aaacaggagc catcaggctc ctgtttgcat ttctgacgac
    20821 ggtgtttatt ccgcgttttc gtgaatatgt ttgctgctga cgggtagtaa accgtcggca
    20881 cggaacatcg ctttaatccc tctgatggcc tgacgaatac ggtcgtggtt ttcaatcagt
    20941 gcaaagcgaa catgcgtatc accatagtcg ccaaagccaa tccccggcga gacacaaacc
    21001 ttcgcttcgt taaggagctt cttggcaaat tccagcgatc ccatggccgc atatggttcc
    21061 gggattttcg cccagacata catcgaagcc ttcggcattt cgaccatcca gcccgcttca
    21121 tgcagccctt taaccaatac atcacgacgg cgtttgtact gttcggcaat gtcgcgcacg
    21181 cactgttgat cgccctcgag tgccgcaatc gccgccacct gcaacggcgt aaaagtgccg
    21241 taatcgtgat agcttttgat tcgcgccagg gcactgacca gcgttttgtt gccgaccata
    21301 aagccgatac gccagcccgc catgttgtag cttttcgaca gcgtaaagaa ctcgaccgcc
    21361 acatcgcgtg cacccggcac ctgcataatt gacggcgctt tccagccatc gtagacgata
    21421 tcggcatagg ccaggtcatg gaccaccagc acatcgtagc atttcgccag cgccacaacc
    21481 ttttcgaaga actccagttc cacgcattgc gcggttgggt tcgacgggaa gccgaggatc
    21541 atcatcttcg gtttcggata actttcacga atggcgcgct ccagttcgtt gaagaaatcg
    21601 acaccttcca ccagcggcac tgagcgtacc tgcgccccgg caatcaccgc gccataaata
    21661 tggatcgggt aacttggatt cggcaccagc accgtgtcac catgatccag cgtcgccagc
    21721 atcagatgcg ccaggccctc tttcgaacca atagtgacga tggcttctga ttccgggtcg
    21781 atttcaacgt cgtagcgatc ctgataccag cgggaaatgg cgcgacgtaa ccgcggaatg
    21841 ccgcgtgaag tggagtaacc atgcgtgtcc gggcgctggg ccacagtaca taatttttcg
    21901 acgatatgcg gcggagtcgc accgtccggg ttacccatgc tgaaatcgat aatatcttcg
    21961 ccgcgccgac gcgcagccat tttcagttca gcggtgatgt taaaaacgta gggcgggaga
    22021 cgatcaatgc gcgtaaagcg acgttcaggg cgagtgtcag ccataatttt ctcagattaa
    22081 cgttagcgcc cggaccgtcc gagcgacgct gccacaatgg tggctgtttt gaaaatagcc
    22141 tgattaattt ctgtctgtcc agcgttttag aacattttta tcaatggatt taaatgagga
    22201 actccacttc aaaaacagga aatttttcta cgctaacaag aaaatcacca ccaattttat
    22261 taacattata atttcaataa atttaccagc tagaagtgca ttatcaagag atacattgct
    22321 aatacaccat ttatctttca atcccctttt aaatttatgg tttttcctca aaaacggcct
    22381 gctggtcatc agccttcagg ttacctatca tagaggttta atccttattc agagtcaccc
    22441 gtgcacgaaa tattcaacat gctgctggcg gtcttcgatc gggcagcgtt aatgcttatc
    22501 tgcctgttct ttctcatccg tatccgcctg tttcgcgaac tgttgcacaa gtcggcgcac
    22561 tccccaaaag aattgctcgc cgttaccgcc attttctcgc tgttcgccct gttcagcacc
    22621 tggtccggcg ttcccgtaga aggctcgctg gtgaacgtac gtattatcgc ggtgatgtcc
    22681 ggcgggattc tgtttggccc gtgggtaggc atcattaccg gcgtgattgc gggtattcac
    22741 cggtatttaa ttgatatcgg cggcgtgacg gcgatcccct gctttatcac cagcattctg
    22801 gcgggttgta tatcaggctg gataaacctg aaaatcccca aagcacagcg ctggcgctgg
    22861 atatcgccaa caaaacgcta ccgctgtttc gccatgtcaa tagcgagtca ttacgcaagg
    22921 tctgcgaaat tatccgcgat gacattcacg ccgatgcggt ggcgattact aataccgatc
    22981 atgtgctggc ctatgttggc gtgggtgaac ataactatca gaatggcgat gacttcatta
    23041 gcccgactac ccgtcaggcg atgaattacg gaaaaatcat cattaaaaac aatgatgaag
    23101 cccaccgcac accagagatt cattccatgc tggtgatccc attgtgggag aaaggggtcg
    23161 tgaccggaac gctgaaaatt tactactgcc acgcgcatca gatcacctcg tcattacagg
    23221 aaatggcggt cggtctgtcg caaatcatct ccacgcaact ggaggtttca cgcgccgagc
    23281 agctacgtga aatggcaaat aaggcagagc ttcgcgccct gcaaagcaaa attaatcccc
    23341 attttctgtt taacgctctg aacgctattt catcgtcaat ccgtctgaat ccggataccg
    23401 ctcgccagtt gatctttaat ctgtcgcgtt atctgcgcta taacattgaa ttaaaagacg
    23461 atgagcaaat cgatatcaaa aaagagctgt atcaaattaa agactatatt gccattgagc
    23521 aggcccgctt tggcgacaag ctgacggtta tctatgatat tgatgaagag gtgaattgct
    23581 gcattcccag cctgctgatc cagccgttgg tggagaacgc cattgtccac ggtattcagc
    23641 cttgcaaagg taaaggcgtt gtcaccatca gcgttgcaga gtgcggaaat cgggtacgca
    23701 ttgcggtgcg agataccggg cacggcatcg ttccaaaggt gattgagcgg gtcgaagcga
    23761 atgaaatgcc gggcaataaa attggcctgc tgaatgtcca tcatcgcgtg aagttattgt
    23821 atggcgaggg gctgcatatc cgccgcctgg agccggggac ggaaattgcg ttttacattc
    23881 ctaaccaacg caccccagtc gcctcacagg ctacgttatt gctttgagcc ggagtgatat
    23941 tgtgaaagtc atcattgttg aagacgaatt cctggcacaa caggaactga gctggctaat
    24001 taaagagcac agccagatgg agattgtcgg cacctttgac gacggtctgg acgtgttgaa
    24061 gtttttgcag cataaccgcg tcgacgccat ttttctggat atcaatattc cgtcgctgga
    24121 tggcgtgttg ctggcgcaaa acatcagcca gttcgcccat aaaccgttta ttgtgttcat
    24181 caccgcgtgg aaagaacatg cggtagaagc gtttgaactg gaggcgtttg actacattct
    24241 caaaccgtat caggagtcac gtattaccgg gatgctgcaa aaactggaag cggcctggca
    24301 acaacagcag accagcagta cgcctgccgc gacggtaacg cgtgagaatg acaccattaa
    24361 tctggtgaaa gatgagcgaa taatcgtcac gccaattaac gatatctatt acgccgaagc
    24421 gcacgagaaa atgacctttg tctatacgcg gcgtgaatcc tacgtaatgc cgatgaacat
    24481 taccgaattt tgcagcaaac tgccgccgtc gcattttttc cgctgccatc gctcattttg
    24541 tgtcaatctg aacaaaatac gcgaaatcga accgtggttt aataacacct acattctgcg
    24601 actgaaagat ctggattttg aagtgccggt cagccgcagc aaagtgaaag aatttcgcca
    24661 gttaatgcat ctttaatcgg gaactttcat gaaagcacca ggcttgcccg ccgatcaaca
    24721 atttttcgcc gatctgttca gcggcctagt gcttaacccg caactactgg ggcgcgtctg
    24781 gtttgccagc cagcctgcct cgttaccggt gggcagttta tgtattgatt ttccccgtct
    24841 ggatatcgtg ctgcgcggcg aatacggcaa cctgctggaa gcaaagcagc agcgaatggt
    24901 ggaaggagaa atgctgttta ttccggcacg cgcggctaat ttaccgatca acaacaaacc
    24961 ggtgatgctg ttaagcctgg tgttcgcacc gacctggctt gggttatcgt tttacgatag
    25021 ccgcaccacg tcgttgttgc atcctgctcg ccagacccag cttcccagcc tgcaacgcgg
    25081 tgaaggtgaa gcgatgctta ccgccctcac ccatcttagc cgttcgccgc tggagcagaa
    25141 tatcattcag ccactggtgt taagtttgct gcatctttgc cgcaacgtgg tgaatatgcc
    25201 gccgggtaat tcacaaccgc gcggcgattt tctctatcac agcatttgta actgggttca
    25261 ggataattat gcccagccgc tcacccgcga gagcgtggcg cagtttttta atatcacgcc
    25321 caatcatctg tcaaaactgt ttgctcagca tggaacgatg cgttttatcg agtatgtgcg
    25381 ttgggtgcga atggcgaagg cgaggatgat tttgcagaaa tatcatctgt caattcatga
    25441 agtggcacag cgttgcggtt ttccggatag cgactatttt tgtcgcgttt tccggcgtca
    25501 gtttagtctg acgccgggag agtacagcgc ccgttttcag ggctaacgtc agaaggttaa
    25561 ttctgtttcc agcagcgtca ggatactttg tgcatcttgc gcggcaaaca acgactggcg
    25621 gaagttttta ttcaccagtt tacgcgccag ctgcgagaag actttcacat gattcatccc
    25681 ttcgttagca cccagcgtca gcatgatcac cagttcgact tcgcccattt ctgactgcca
    25741 gtcaaccggt ttcgccagcc gggcaatgct gatactggaa tgacggatcc actgagattt
    25801 ggtgtgcggg atcgctacgc caaaaccaac gccggtggta acaatctctt cccgctgcca
    25861 gacatcttct tccagctcaa acggatgttc agtgcgcccg ttaacgccga ggttgccgca
    25921 caggaactgg atcgcctgct ctttattgct aaaatcctga tcaacaaaga tattctccag
    25981 cgccagcagt gggcgaacgt cttcttccgg cgtaaaggcg gtgagtaacg cttcaatttc
    26041 ctgcgcactg cggcattcac atgcctgacg cgccagttcc cgacacgcct cgctatccag
    26101 ttgacgaagc tggcttttca ccgccggaat acgcgggcta ctcatactca gctcatccag
    26161 gcccagccca agcagtagcg gcagataacg gctttcaccg cccagttcac cgcaaatgcc
    26221 tacccatttg ccccgctgat gcgcagtggt aactatttgc tgcaacatgc gcaggaacga
    26281 tggcgtaatc gggttatata gcggcgatac gcgcgggtta ttacgatcga ccgcatacag
    26341 atactgggtc atatcgttgg agccgatact gaagaaatcg acctcatcgc agaagtggtc
    26401 gatgatgtag cacaccgacg gcacttccac catgatccca agcgtaatcg tctctgcatg
    26461 acgcaggcca tcgcgcttaa gctcaacgat cgctttttgg atctcgcctt tcacccataa
    26521 gatctgatcg aggctgtgaa ccatcgggat catcaactgg gcgttgccga aactggcggc
    26581 gcgcaaaatg gcccgcagtt gagtgcggaa caggccagca aattccgggt aaatacgtac
    26641 cgcgcgatag ccgaggaacg ggttctcttc ctggggaata ttcagataag gaatgctttt
    26701 atcgccgccg atgtccatcg tgcggaagat aatcggcttg tcgcccgccg ccagtagcac
    26761 ctgctggtag gcttcaaatt gctcctgctc gtccggcgcg ctgtcgcgat ccatatagag
    26821 catttcggta cggaacagcc cgacaccttc cgcgccgttg gcaaacgcgc ctggcgcttc
    26881 cagagcggtg ccgatattcg ccgcaatatc aatacgcttg ttatcacggg aataggccag
    26941 ctgcgcggca gcctgcgcct gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg
    27001 ataataaccg ctcaccgcgt cattcgggtt aatcgccagc acgccgcact gggcgtcaag
    27061 cactgcaggt tgcccggcat aacgggcaat cgcatccagc ggcaagccac tcagtaccgg
    27121 gatcgccgag gcacgggcca gaatcagtgt atgcgaggtg cgcccggttt tctccagaat
    27181 catacccgca agatttttca aatcaaggct caaaaactgg cttggggtta aatcttcagc
    27241 caccagaatg gtcggttttt ccagcaccag attgttgcgc ggcttcagtt ccggccaggt
    27301 gatatgcagc aactgttcgc tgatatcgcg aatgtcgctg acccgctcgc gcagataatc
    27361 gctggcagaa gcagatagtt tggcgcaaac ctgctccata ttgctgatga tcgccgcccc
    27421 cagtccctga tgctgttctg tcatcaggcg acggatattg cctgcaaatt catcatcctg
    27481 aatcagcgac aaatgagcgc tgaggatagt tttgctttcg ccgtcacgct cacgcaattg
    27541 ctgattcagt tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc
    27601 actggcgggg attgcccgat aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc
    27661 cacgccgacg ccgcttgcca gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa
    27721 tgaacgcggc agcggatgcg ccgtcagttc tgcctgcgtc ggctgaacgc tgtcgctgtc
    27781 gataaagcgc accaggatgt actcttccag cacgcgccgc gcctgctctt catcgctgcc
    27841 gctaatgttc aggctgcaac tgtcattaaa taaggtgcca gttccaatca acgccagcga
    27901 gcttttagca tcagcctttg cgttctggcg atggttgata aaggtgattt cgctttgcca
    27961 ctggctgcac tgttctttaa gttcccacgc cggacgagca tgtagaccgt taggcaaagg
    28021 acagagaaat tgaatcgtta acataaccgc tccgggttaa tcaggagatc atctgaaatc
    28081 cgtcagttga acaaccgtct cacgcgtaag acgttgaata agggcagata aaagttgctc
    28141 catctgcaaa atgtcgcggc aatcggcaat cgatgccgcg caatgtccgt ggcgggtggc
    28201 aggccctatt accaatgtgg gtacgccagt accggttaag tgcaccgctc caccgtccgt
    28261 gccgccgtta ctgaacatat ccgcctgcaa cggaacgcca atttctgccg ccacggtttc
    28321 aatccacgcg gtaagttttg gcggcgcaat cagcgattta tcgcttaaca ccagcatcgg
    28381 cccgttacca atctgacgat ggttagccgc gccataatca aagtttttcg cccagcaggc
    28441 ggtatcaagc acaatggcga cgtccggcga caccgcgcgg gtagcagttt gcccgccgcg
    28501 taatcccacc tcttcgctgg aacttgccac cagccacact tccgcaggca gttcggcgtc
    28561 gtgcagctca cgtagtaacg tcaccagcag atagcaactg aggcgatcat caaaggcttt
    28621 gcccatcacc cgctggtgag ggaggacctg aaaagtggta tcgaaggtga cgcgatcgcc
    28681 tggacgaatt cccgcctgca tcacttcgtc ataggtgcgc gcgccaatgt ccacgcgcat
    28741 ggcgctaacg tcattcccct gccggtcgcc gtcaagcagg cctggaattt tgcactcttc
    28801 acgggtggtg atgcgcaccg gctgcagctg gcgggcagcc atgcgcacgt tgccaaccgg
    28861 cagcacgtca atagcgcctt cgcgggagat gctgcgcacc ataaatccca cttcgtccat
    28921 atgggcgcag atcatcacct tcggacctgt cgattcattg aggcggatca gcaccgatcc
    28981 cagaccatca aatcgcactt ctttttgcag gcgagccgct tcttccagca ggatctgccg
    29041 cacttcctgt tccgaggagg cgatcgcatc tgcctcgctc aacgctttta atagcgataa
    29101 atccattatg cctctcccgt gagcaacact gttttcggca tggcgtagag cacttctgcg
    29161 ccttgcgggg taaccagcac aacatcttcg atgcgcacgc ccccttgccc tagcaaataa
    29221 atccccggct ccacggtcag caacatgcct ggctgtagcg tcgtggtgtc ccgcggtgaa
    29281 aaacgcggat cttcatggac ttcaatgccg atagcgtgac cagtgttatg accgaaatag
    29341 tcgccaaatc cagcctcggt aatgacccgg cgcgcggcgt cgtcaatctg ctggcagcgc
    29401 acgccggggc gaattgcaga gattgccgcg agctgtgcct gcaggacaat ctgatagacg
    29461 tcaaacagcg ggtgagattc ggcgctcacc ccttcgccat tcaccagcaa ggtgcgcgtc
    29521 atatcagcag tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc
    29581 aacaatcttg tcgctggctt tgccgtgcgg cagcgccccg cgccagccgc tggcgacaat
    29641 ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc
    29701 tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc
    29761 ggcaatccca caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag
    29821 cacatccggc gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt
    29881 ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat
    29941 ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg
    30001 cgcacgggct tccacctcaa cgaaatagcg cgaatctacc agaatgtgcg cactttcacg
    30061 gctaataacc acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg
    30121 tgaggagaga agcaccgcat ccagttgttg caccttaagc cagttgcgca gcgaagcgag
    30181 taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc tttgcggaac
    30241 atcatcagac gcaggaacac caccatcagc gcagtaatga ttgcccccag cgcgatcccc
    30301 gccatataaa cgcccaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac
    30361 cattgcactg cgcccagcca gacagctgcg gttgagccga caatcgcgcc gaccatatat
    30421 gacggaatag cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc
    30481 ataaaggcca ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt
    30541 ttgccggtta aacgacggtc aataatggtc gccagaccca gaccaatcgg cggaataacg
    30601 atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg
    30661 aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc
    30721 attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacagtacg gataccgccg
    30781 ttgatccagc caccaaaggg agtgatgacg tagtacatcg ccagcatgac aaaaatggcg
    30841 gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt
    30901 ttctggttca tccatttcac aaggtaaccg gcaacgatag aaataatcag cgcaccaatg
    30961 aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc
    31021 agttgcgtcg gctgggtaga catcaacccg ccgataaaac cagccgggaa cgccagtttg
    31081 ccaccgatag agttcgccac aaatgcagca aacatcggaa tggcaaaacc aaacaacacg
    31141 ccgccgaagg actgcgacag ccaggcaaat ttaagtaagg agaggtcgaa accggagaat
    31201 ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa
    31261 gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt
    31321 gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc
    31381 ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt
    31441 ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct
    31501 tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa
    31561 acgtcgcgtg attcgaaacg ttcgttatct tccggggtaa ctgccacgga atggatgatg
    31621 atggtcgctt cggcgatatc ctgcgccgtc aggcgattct ggatgccgtc cgcgccctgg
    31681 gtttcaattt tcacttcata accggcttct accgccgctt cttccagcac ctgagcggcc
    31741 ataaaagtgt gagctaggcc cattgggcag gcacataagg caatcagttt cttactcatc
    31801 gttaattcct cgttcagtga tagttgacga gtgaaattat gaacgtgcaa aacaaatcgc
    31861 cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg
    31921 caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaaagat
    31981 tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtga cctaaggtct
    32041 ggcgtaaatg tgcaccggaa ccgagaagac ccggatagtc atggacgatg agatacaccg
    32101 gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcggca cggaaaccgg
    32161 aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa
    32221 atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg
    32281 acaatgcgcg gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta atatcttttg
    32341 gcttgagatt ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca
    32401 gcccagggcc agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt
    32461 cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg
    32521 gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cccgttccgg
    32581 caccgtaaac cgcaataggc ttaccttcga ccggttctgc gccaccaaac tgaatcagat
    32641 gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca
    32701 gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg
    32761 tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct
    32821 cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc
    32881 ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg
    32941 cgttggtgcc gcccacatca ccgactaatg catactttgt cattcttcaa ctgctccgct
    33001 aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga
    33061 aaggcaggct ccctgtaaat atcgatctgg gtcacacaat tactttatcg tttcagcacc
    33121 aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa
    33181 tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca
    33241 attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag
    33301 aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc acccctctgg
    33361 atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag
    33421 ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccggt tccgccctct
    33481 gcgctacctg gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg
    33541 ccggaacatc cgatcatgac cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg
    33601 ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg
    33661 ctgtttggta cacctgttgc ggcggcgttg atattttcgc aaaccttaaa tggcagtagt
    33721 gaagttccgc tatgggatcg tctttttgcg ccgttaatgg cggcagcagc tggtgcactt
    33781 actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg
    33841 gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg
    33901 gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg
    33961 ctcgtgctgg gtattggcgg atttattctc ggaattctgg gggttattgg tggaccagtt
    34021 tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc
    34081 agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt
    34141 ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg
    34201 ctgcatgagc acgttcccgc cgttccagcg gcaataaccg tttcctgcgc tattctcggc
    34261 atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc agtcgttgta
    34321 cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta
    34381 gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataatgtt
    34441 tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct
    34501 ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg
    34561 ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaatgca
    34621 ggtgaaatca ccctactgcc atcgatcaaa ttacaaattg gcgatcgcga tcattacggt
    34681 aattactggg acggtggtca ctggcgagat cgtgattact ggcatcgcaa ttatgaatgg
    34741 cgcaaaaacc gctggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa
    34801 gcatatgagc gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc
    34861 cgcggacatg ggcaccgcca ttaaacgcgt cctccaatgg agcacaatgc ctgatgcgac
    34921 gctagcgcgt cttatcaggc ctacaaaccg gctctttaac gcgtagggcg gataaggcgt
    34981 ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    35041 gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    35101 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    35161 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    35221 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcaacat ggtgactgta
    35281 cgacgcaccc acagcgggat atggaagcga atgaatccct gcatcaccac ctgccccgcc
    35341 agtgtcccca ccaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    35401 gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca
    35461 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcac cagattgaca
    35521 aaaccggcaa tcgtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    35581 cgcgaaccgc catgtaaatg ctgagtgagc gaagagtgca aataaatcac atgcggcata
    35641 atcgtcgccc ctaacacgcc tgctgccagg aagaccgcct ccgaagtagg taaactcggg
    35701 atcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    35761 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgg tttttgcccg
    35821 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    35881 aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    35941 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    36001 tgatcacgaa tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg
    36061 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    36121 aagctagcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    36181 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    36241 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    36301 gctatgctaa acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctctga
    36361 tagaccaaga ttatgattac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    36421 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat
    36481 cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    36541 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    36601 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gtgattctcg cactgctggt
    36661 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    36721 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    36781 cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag
    36841 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    36901 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat
    36961 tggtttcctg ctctccaaag tcaacggtat gggcaaactg gaatccttta acgccgtcag
    37021 ctccctgatt ctgggtcagt cagaaaactt tattgcctat aaagatatcc tcggcaaaat
    37081 ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat
    37141 cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtactgaa
    37201 catgttcagc acctttatcg tgctgtcgct gatcaatcct taccgtgttg atgccagtga
    37261 agaaaacatc cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    37321 atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat
    37381 cgccctgatc gccgcgctga acgcactgtt tgccaccgtt actggctggt ttggctacag
    37441 catctccttc cagggcatcc tgggctacat cttctatccg attgcatggg tgatgggtgt
    37501 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    37561 gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg
    37621 catcatctct gtgttcctag tttccttcgc taacttctct tcaatcggga ttatcgcagg
    37681 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact
    37741 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    37801 aaaccataca taaaaaagcc ggggatcatt ccccggcttt tttacgcctg ttaatcagct
    37861 aatggctgcg ggcgaccaat caaataccct tgcagatatt gcaccccgag cttatgcaat
    37921 agcgcctgct gctgttgcgt ctcgacaaac tccgcgacca cactcaatga cttcgctttc
    37981 gccagatcgg taattgatct cacaatcatc gcatccagcg tattcgtgac aatatctttc
    38041 acaaagacgc catcaatttt gatgatatca gcctgcaaac gctttaaccg ttcgtagttg
    38101 gcatatccgg tgccaaagtc atcaatcgca atccggaaac caaacttatg cagctgctcg
    38161 atgttgtaca tgctgctttc tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg
    38221 accgcctgcg gggagatgtg ataacgctta aacagacgaa taatccgccc ggcaatattc
    38281 ttttgcagca gcgtgagcgg cattaaattg actgaaaagc gcggtccttt tttgtcgcaa
    38341 gggtgtgttg ccagccactt caacaaggat tccagcactt gcaaatcaaa acgcgcgcta
    38401 aggttaaact gagcaataag gggcagaaac ttatccgggg tcataatgcc gccgtcatat
    38461 ttcagtcgcg cgaggatctc atcataacct tcaccctctt tgttgcgaat tggctgggcg
    38521 tagagcagca aatcaccctg atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt
    38581 ttagtggtct gcccggaaac catctcctct ctgctatcca gcgccagcac atgatgatgt
    38641 gcgcaggatt gctccgccag ccagcttaac tgccccaaca agggttgcag ggtttcctga
    38701 tttccatcaa aacgccccca ggcagcacca tagcccatat ccagcccggt attgttccag
    38761 tgaatttgcc gactattcag gatgttaacc atatgctgga gtcgcccttc cgtttccggc
    38821 ccgctcagca ctaacagcag ttcactaccc ggcaattgat acaacttttc gttttcctgc
    38881 atcaacggca gcagcgtacg gcaaattgag cggatacagt gaacgcgcat cattaagccg
    38941 taatgacgac tcataaactc aagattatca atgcgcaggc agcaaaaact cttgcccgcc
    39001 tcttgctccg gcgcttgttc caacgcacga aagttgggta ggagcgttaa cggatctgtt
    39061 agcgcctgca aatgccagcg gcgattaagc cattcactgc gatgataaat gcgcaccata
    39121 tagagcaggc aaacgctaaa ggaaatcagc accgcgagaa taaatgccag cgaatattcg
    39181 gtttccaccc cttgcaaaaa gttctggttg taattcagaa ggcaaagcgt tgaaacagcc
    39241 caggtgagat ttaaaaacgg atagcgaaga ttaccgaccc cgagggtaaa gatgatgaag
    39301 aaaacgggta ccaggtaacc ggcaataaag tcgttttcat aaggtgtgca caacagaagt
    39361 agcagcacgc ttaacgctgc cagccagctt aaggtaaatg cgagtgagat agtagaaaag
    39421 catgttgtaa atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc
    39481 atccgcatcg ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag
    39541 atacatgctg catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca
    39601 gacatatcgg ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc
    39661 gcaggcacac aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga
    39721 gagatgaaag ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg
    39781 ccagccaaag ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat
    39841 ataactggaa tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac
    39901 ggtgagcgta aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa
    39961 catgagtaca ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg
    40021 catcgcgaag caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc
    40081 aggcggcgct aacccggacg tagactgtat tacaaaagcg gcaaaaagca gagacaaaaa
    40141 acccccgctt tgcagcgagg gttg
//
LOCUS       DAJTUN010000022        39967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTUN010000022 DAJTUN010000000
VERSION     DAJTUN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.022"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO470_001108"
     CDS             complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO470_001108"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6250427.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="OO470_001109"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="OO470_001109"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6250428.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO470_001110"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO470_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6250429.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO470_001111"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO470_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6250430.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO470_001112"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO470_001112"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6250431.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO470_001113"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO470_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6250432.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15088)
                     /locus_tag="OO470_001114"
     CDS             complement(14756..15088)
                     /locus_tag="OO470_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6250433.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(15073..15480)
                     /locus_tag="OO470_001115"
     CDS             complement(15073..15480)
                     /locus_tag="OO470_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6250434.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15477..16040)
                     /locus_tag="OO470_001116"
     CDS             complement(15477..16040)
                     /locus_tag="OO470_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6250435.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16031..16501)
                     /locus_tag="OO470_001117"
                     /pseudo
     CDS             complement(16031..16501)
                     /locus_tag="OO470_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16685..17479)
                     /gene="thyA"
                     /locus_tag="OO470_001118"
     CDS             complement(16685..17479)
                     /gene="thyA"
                     /locus_tag="OO470_001118"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6250436.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17486..18361)
                     /gene="lgt"
                     /locus_tag="OO470_001119"
     CDS             complement(17486..18361)
                     /gene="lgt"
                     /locus_tag="OO470_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6250437.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18512..20758)
                     /gene="ptsP"
                     /locus_tag="OO470_001120"
     CDS             complement(18512..20758)
                     /gene="ptsP"
                     /locus_tag="OO470_001120"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6250438.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20771..21301)
                     /gene="rppH"
                     /locus_tag="OO470_001121"
     CDS             complement(20771..21301)
                     /gene="rppH"
                     /locus_tag="OO470_001121"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6250439.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21986..22675
                     /gene="mutH"
                     /locus_tag="OO470_001122"
     CDS             21986..22675
                     /gene="mutH"
                     /locus_tag="OO470_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6250440.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22744..23457
                     /locus_tag="OO470_001123"
     CDS             22744..23457
                     /locus_tag="OO470_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6250441.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23595..23813
                     /gene="ygdR"
                     /locus_tag="OO470_001124"
     CDS             23595..23813
                     /gene="ygdR"
                     /locus_tag="OO470_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6250442.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23921..24961
                     /locus_tag="OO470_001125"
     CDS             23921..24961
                     /locus_tag="OO470_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6250443.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24994..26184)
                     /gene="lplT"
                     /locus_tag="OO470_001126"
     CDS             complement(24994..26184)
                     /gene="lplT"
                     /locus_tag="OO470_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6250444.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26177..28336)
                     /gene="aas"
                     /locus_tag="OO470_001127"
     CDS             complement(26177..28336)
                     /gene="aas"
                     /locus_tag="OO470_001127"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6250445.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28922..29953
                     /gene="galR"
                     /locus_tag="OO470_001128"
     CDS             28922..29953
                     /gene="galR"
                     /locus_tag="OO470_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6250446.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29960..31222)
                     /gene="lysA"
                     /locus_tag="OO470_001129"
     CDS             complement(29960..31222)
                     /gene="lysA"
                     /locus_tag="OO470_001129"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6250447.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31344..32279
                     /gene="lysR"
                     /locus_tag="OO470_001130"
     CDS             31344..32279
                     /gene="lysR"
                     /locus_tag="OO470_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6250448.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32266..32958)
                     /gene="ygeA"
                     /locus_tag="OO470_001131"
     CDS             complement(32266..32958)
                     /gene="ygeA"
                     /locus_tag="OO470_001131"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6250449.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33087..34505)
                     /gene="araE"
                     /locus_tag="OO470_001132"
     CDS             complement(33087..34505)
                     /gene="araE"
                     /locus_tag="OO470_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6250450.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34820..35581)
                     /gene="kduD"
                     /locus_tag="OO470_001133"
     CDS             complement(34820..35581)
                     /gene="kduD"
                     /locus_tag="OO470_001133"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6250451.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(35611..36447)
                     /gene="kduI"
                     /locus_tag="OO470_001134"
     CDS             complement(35611..36447)
                     /gene="kduI"
                     /locus_tag="OO470_001134"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6250452.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36734..37915)
                     /locus_tag="OO470_001135"
     CDS             complement(36734..37915)
                     /locus_tag="OO470_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6250453.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38170..39399
                     /locus_tag="OO470_001136"
                     /pseudo
     CDS             38170..39399
                     /locus_tag="OO470_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         9917 a        10805 c         9700 g         9545 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
      301 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct cttcctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgctcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtct tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgt tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gattcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgt gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcaa cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcggttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaat cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaagcct ttatcagtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcggata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta tgaaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctaa
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gagttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    14941 actggtttct gctggcgaga ctgttcattg atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcagcacca tcgcaaccat tacctccggc aggctaaagc
    15061 cttgttgatt cttcagggaa gctgacataa tgccccctct ttcagcggac aaaaatcgct
    15121 ccagccgcgt ggcgaaaaaa caatttttcc atcaatgact tcgcctgttc gccacaacga
    15181 aacgccttca taaccggcaa tcaataaggc ttcattatct gccagtaaac gcaaacaaac
    15241 ctgggcatcg gttccagcgt actgcgagca ctgaactgct ggctgcgtct gccaggagtg
    15301 catttttccc cacgccagcg ccgactgaac gatggcctgg cggcgcaatg actgactttc
    15361 catgctcacg cgagaggcaa aactgcgatc ctgctgactc attccttgca atagcaagct
    15421 acccaaaacc agcagcatca ggaccagagc cagtgacgaa acaccttttt cgcggttcac
    15481 aggttgaatc ctgtcacgct atagctggca tccaccacgg tttgcggttc agacttactg
    15541 gcagcacgca tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc
    15601 tgaaaagtgt cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca
    15661 caggatgtcg caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg
    15721 tcggactctt ttaccggttc gcgatcccag ataccgttac tgttcgcatc ccactgcaca
    15781 atgatacagt caccctgtcc gacaatttcc agcccttcgc cggtacagct gccatgacaa
    15841 taacccgccc tctggagatg cttcgcgacg gtaaataccc gcagccagat ttcatcttcc
    15901 agtgccagct tacgagtgct cgttaaactt tcacgctgta acgcaggcag aaagcgtgca
    15961 gcccccagca acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa
    16021 ccttgctctt ttacaggcat ccttctgttt ctccttgctg ataaagccgg agtcttcccc
    16081 acggcgaaac caccagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc
    16141 atgcggtatt acgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt
    16201 cgggccagcg tggcacaaag accaatggtg aactgccatg acaggtattg gccccagcag
    16261 cggaactcac aaggcaccat aacgtcccct ccctgataac gctgatactg tggtcgcggt
    16321 tatgccagtt ggcatcttca cgtaaataga gcaaatagtc ccgcgcctag ctggcggttt
    16381 gccaaagccg ttgcgactgc tgccagtatt gccagccata gagtccactt gcgcttagca
    16441 tgaccaaaat cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgtgttttca
    16501 tgccggcagt atggagcgag gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc
    16561 atcttccgga ctatttacgc cgttgcagga cgttgcaaaa tttcgggaag gcgtctcgaa
    16621 gaatttaacg gagggtaaaa aaaccgacgc acactggcgt cggctctggc aggatgtttc
    16681 gtaattagat agccaccggc gctttaacgc ccggatgtgg atcgtagcct tcaatctcaa
    16741 agtcttcgaa acggtagtcg aagatggatt cgggtttacg tttgataatc aacttcggca
    16801 gcggacgcgg ttcgcggctt aattgcagat gagtttgatc catatggttg ctgtacaggt
    16861 gcgtatcgcc accggtccag acaaaatcac ccacttccag atcgcactgc tgcgccatca
    16921 tatgcaccag taacgcgtag ctggcaatgt tgaacggcag gccgaggaag acgtcacagg
    16981 agcgctgata aagctggcaa gagagtttgc cgtctgccac atagaactgg aagaatgcat
    17041 ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa acaataatgc
    17101 ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt agtaatctgg tcaatatgac
    17161 gaccatctgg cgttggccag gcgcgccact gtttaccata cactggcccg aggtcgccat
    17221 tttcatcggc ccattcgtcc cagatggtga cattgttttc gtgtagataa gcaatgttag
    17281 tatcgccctg caggaaccac agcagttcgt ggatgatgga acgcaggtgg caacgtttgg
    17341 ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat ctgatgacca aaaatggaaa
    17401 gcgttccggt tccggtacgg tcgtttttct gtgtgccttc gtcgagcact ttttgcatca
    17461 gttctaaata ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg cgacgatatg
    17521 cccagaccat catgatcaca cccgcgacaa tcatcgggat ggaaagaatt tgccccatgc
    17581 tgatgtactg cacccaggca ccggtaaact gcgcgtcggg ctggcggaaa aactcaacaa
    17641 tgatgcgaaa cgcgccgtag ccaatcagga acaagcctga gacagctccc attgggcgcg
    17701 gtttacgaat atacaggttg aggataataa acagcaccac accttccagc agcagttcgt
    17761 aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat tgccactgcg
    17821 ggttggtttg cagcagcaaa atatcttctg tacgggagcc tgggaacagc atggcaaacg
    17881 ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat aaagttgccc agacgcccgg
    17941 caccaagacc aaacggaatg agtggtgcga taaaatcaga gacctggaag aaggaacgtt
    18001 tagtacggcg ggcgaagata atcatcacca cgataacgcc aatcaggccg ccgtggaaag
    18061 acatgccgcc gtcccagaca cggaacagat acagcggatc ggccataaac tgcgggaaat
    18121 tgtagaacag aacataacca atacgtcccc cgaggaagac gccgaggaag cccgcataga
    18181 gtaagttttc aacttcattt ttggtccagc cactgcccgg acgattcgcc cgacgtgttg
    18241 ccagccacat tgcaaaaatg aaacccacca gatacatcag gccgtaccag tgaagcgcca
    18301 cgggtcctat tgagaaaatg accggatcaa actccggaaa atgcagatag ctactggtca
    18361 tctgtcacca caagttcttg ttatttcgct gaaagagaac agcgattgaa atgcgcgccg
    18421 caggtttcag gcgctccaaa ggtgcgaata atagcacaag gggacctggc tggttgccgg
    18481 ataccgttaa aagatatgta tatgatccgc gctataaccc tccgcgaatc agcccgccca
    18541 tgccgcgacg ctccataaag gctgcaacct gatgacgaac ttcggtcgcc agttgcgctt
    18601 ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc aatgcgccgc agcaggtatt
    18661 ttacccgcgc tacagaacgt ccgttcatag acagatggcg atacccaagc ccaatgagga
    18721 ttgccacgca catgggatcg cccgccattt caccgcacaa acgaagatcg ataccatgta
    18781 tttccgcttc ccgggcgatc atcgccagag ctcgtaacat tgcaggatga agactgtcgt
    18841 aaatgttcgc cacccgggta ttgttgcgat caacggccag aatgtattga gtcagatcgt
    18901 tggtgccaac agagatgaaa tcgacccgct ttgccagatg cggcagcata aataccattg
    18961 acggcacttc cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg atcatctcct
    19021 cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc gtcaacttca tcgaggctgg
    19081 tgaccatcgg caacagaata ttcaggttgc ccgtagcggc attagcacgc agcatcgccc
    19141 gcacctggat caagaagatc tccggctgat cgagcgtaat gcgaatccca cgccaaccca
    19201 ggcatggatt ctcttcgctg atcggcatgt aaggcagctg cttatctgct ccgacatcca
    19261 gcgtacgcaa ggtgacgggt ttatcattga acatttgcag catcccctga tactgcgcca
    19321 cctgttcttc ttccgacggg aaaccacttt gcagcatgaa tgggatttca gtgcgataaa
    19381 gaccgatgcc atcaatacag ctgcccagtt tttcttcatg ttccgggctt aaaccagcat
    19441 tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc gggtaaattg acgtcatctt
    19501 ccgccagacg gctaagctca atctcttcac taattagccg ctgatattct tgcagcagta
    19561 ccggctccgg atcgaccagc aattcaccgc gatagccatc aacgatcagc gtcctacgat
    19621 gcagcaccga aggctgaata tccgcgccca tcacggtagg gatccccagc gcacgtacca
    19681 tgatcgcagc atgggagttg gctgagccat ctcgcacgac aacaccgact aagcgatcct
    19741 ggggcagctc agcaagagtt gtcgctgaca gttcatctgc caccagaatg aaacgttccg
    19801 gccaggcgtt cggcccttga ttagcgtcat caagatgaaa cagcaatcgc tgacccagcg
    19861 cacgtaaatc gccagcccgc tctttgagat agttatcgct tagcgcggca aactgttcgg
    19921 caaatttttc aatgaccgtt tttaccgccc actctgccac cgagccttta tcaacctcgg
    19981 caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg cgagtaaaga tcgaaaatag
    20041 ccgccgtttc tttttgtgct ccggcggcaa agcgtttgct gtagcggcga aactcgtttg
    20101 ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag agccggatcc agcgttgatg
    20161 cctgatacac ctgttccatt aaaggtaacg tggcatcctg ccagccttcg gcaatcgcca
    20221 caccaggtgc tgccggtaat gcgcggattc gcgtctggcg atattgccca aacagggcag
    20281 tcaactgcga ctgagaaaga atagctgcca tctgggtggc aagcgtcacc aggaaggatt
    20341 cttcactttc gtcatactgg cgcaactctc gttgctgtac caccagtaca ccaagcaact
    20401 ggcgacgttg aataattggt acgcctaaaa acgcgcggaa acgttcttct tttacggagg
    20461 ggatgtattt gaagctgggg tgcttttgcg catctgcaag gtttatcggt tccgccagcc
    20521 tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt tacagtgcga ccgcgtggtt
    20581 ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg gccaggtaga
    20641 ccgaacagac ctcggtatcc atcgcaagac agatgtcggt aaccagaata tttaacgcct
    20701 cattcaggcg tggtgcgctg gctacctttt cgactatttc gcgcaggcga gtgagcataa
    20761 ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc gttttgtggt tttggcgtat
    20821 tttcctgcag tgacatcacc acactcgcga actctttcat taccctacgg tagacatcac
    20881 gtttaaatga caccacctgt ctgaccggat accagtaact tacccatcgc cagccgtcaa
    20941 actctggtgt actgctggtt tgcatattga tttctgcatc gccgcttacc agctgcaaga
    21001 gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc accaaacgtt
    21061 tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg tctttgcggc
    21121 ttaatcctac ttcttcaaac aattcacggt acatcgcctg ctctgcggat tctccggggt
    21181 tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc cacattacct
    21241 gcccctggcg attacaaatc acgataccta cgtttgggcg gtagccatcg tcatcaatca
    21301 ccggactacc tcaaaataaa gctttatata cgaatgattg tttcatactc caggaagacg
    21361 gtaaaccact ctctgcaggg cattacacac taataacaat tgaataactc acagttatgt
    21421 gcagagttat aaacagagga aggggtggat agccgttttt attcaccttt tctgtggata
    21481 gagttgtgaa gaactacgga attactacgg gaaaacccgg agcattctga ataagcccga
    21541 tatagaaatt ttaaaatatt cattattagt caatgcatta catgtaattt gagtaatgaa
    21601 aaagagtaat tcgtgaccca ggtcacacct ctcatttacg ggttggcgaa agatcaacca
    21661 atgccgtatt tatccacaga atgtgccact aagttaagca ctgaaccact aaaaactgga
    21721 gtttcgtcgc acgtcaaggc tgtaaatgaa aacagtagtg gaggtttttc acagttatcc
    21781 cagctttctg tggataacat ggtgtaagat cctgtttatt ttcagtgacc agatttggaa
    21841 aacctattgc agtgttgcgc aactcgatta ccggcagcct aaaaagcaat ataaatcaga
    21901 gaattgaaca acacatgtgg aaaaagttac actgcgaata ttcggcacat aattactgtt
    21961 tgttttttaa ccaaggtatc atgacatgtc ccaacctcgc ccactgctct ctcctcccga
    22021 aactgaagaa cagttgttag cgcaagcaca gcaactttct ggttatacat tgggagaact
    22081 ggcggcactt gccgggctgg ttacgccaga gaatttaaaa cgcgataagg gctggattgg
    22141 cgtgttactg gagatctggc taggtgccag cgcagggagt aaacctgagc aagattttgc
    22201 tgctctgggc gtggaactta aaactatccc tgtggatagt cttggtcgtc cgctggaaac
    22261 aacattcgtt tgtgttgccc cgttaacggg taatagcggg gtgacctggg aaaccagcca
    22321 tgtgcgccac aagctcaaac gcgtactgtg gataccggtt gaaggcgagc gcagcatccc
    22381 gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag aggaagaccg
    22441 acagctacgc gaagactggg aagaattaat ggatatgatt gttctcggtc aggttgagcg
    22501 gatcaccgct cgtcacgggg aatatttaca gataagaccg aaagcagcga atgcgaaagc
    22561 gcttaccgaa gccattggtg cccggggcga acggattctg acgctgccac gcggctttta
    22621 tttgaagaag aatttcacca gtgcactact ggcccgtcat tttctgatcc agtagccatc
    22681 gctttgacct gccgctttcc gggcatataa ttaccgcttc atttttttgg cagggctttt
    22741 tagatgttat ttgcatggat aaccgatcct aacgcttggc ttgcgctcgg tacgctgacg
    22801 ctgctggaga tcgttcttgg gatcgacaat attattttcc tttctctggt ggtggcaaag
    22861 cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc catggttatg
    22921 cgtctggcgc tgctggcatc aatctcttgg gttacgcgcc tgacaaatcc gctttttaca
    22981 atattcagtc aggaaatttc tgcccgtgat ttgattctgc ttctgggtgg cttgttcctt
    23041 atctggaaag ccagcaagga aatccacgaa tccattgaag gtgaagaaga agggctgaaa
    23101 acacgcgttt catcattcct cggcgctatc gtgcagatta tgctgctgga tattatcttc
    23161 agcctcgact cggtgattac cgctgtgggt ctgtcagatc acctgtttat tatgatggca
    23221 gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg tgattttgtc
    23281 gaacgccatc cttcggtaaa aatgctggcg ctctctttcc tgattctggt gggctttacc
    23341 ctgattctgg aaagtttcga catccacgta ccgaaaggtt acatctactt cgcgatgttc
    23401 ttctctattg cggttgaaag cctcaacttg attcgcaaca aaaagaatcc gctctgatac
    23461 tccgtacgct ctcctgcggg agggcgtact cactcctttt accctcacag tttaagattt
    23521 tcctgctttc aagatatata acgtcggttt ataaacagac tattatcata ggtaggccaa
    23581 atgaggataa ccggatgaaa aaatgggcag taataatttc tgcagtcgga ctggcgtttg
    23641 ctgtttccgg gtgttccagt gattacgtca tggcgaccaa agatggccgt atgattttga
    23701 ccgatggaaa acctgaaatt gatgatgata ccgggctggt gagttatcac gatcagcaag
    23761 gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt taacaaataa
    23821 ggtcagcatc cggctggcct taagattttt ctctttccct tttcccttcc ctctgccatt
    23881 tttatattcc ttatgtcgtg attataaaaa ggaaacggct atgcaatatc accgtatacc
    23941 ccacagttcg ctggaagtca gcacgctggg gcttggcacg atgacgtttg gtgaacagaa
    24001 cagcgaagcc gacgcccacg cacaactcga ctatgccgtc gctcagggca ttaaccttat
    24061 cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa acgcaagggt taaccgaaac
    24121 ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa aagttaatta tcgcctccaa
    24181 agtgagcgga ccgtcgcgca ataatgacaa gggcatccgc ccggatcagg cgctggatcg
    24241 gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgc ctacagactg attacctcga
    24301 tctttatcag gtgcactggc cgcagcgccc gaccaactgc ttcggcaaac tcggttatag
    24361 ctggacggat tctgcgcctg cggtttcgct gctggatacg ctggacgcac tggcagagta
    24421 ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac gaaactgcat ttggcgtaat
    24481 gcgctacctg catctggcgg acaaacacga tctgccgcgt attgtcacca ttcagaaccc
    24541 ttacagtctg ttaaaccgca gttttgaagt aggtctggca gaagtcagcc agtatgaagg
    24601 ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg ctgaccggga aatatctcaa
    24661 tggtgcaaaa cccgctggcg cacgtaatac gctctttagt cggttcaccc gctatagcgg
    24721 tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc gccagacgtc atggccttga
    24781 tcctgctcag atggcgctcg cgtttgtacg ctgtcaaccg tttgttgcca gcactctgct
    24841 gggcgcaacc acgatggatc agctgaaaac taacatcgaa agtttgcatc tggagttaag
    24901 cgaagacgta ttagctgaaa ttgaagcggt acatcaggtt tatacttatc ctgcaccata
    24961 aagattaacg ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag
    25021 cgccgttatt gccagcgcaa atagcgcacc gaagccaatg ccaatgggca cgaccgggat
    25081 gcctaccatt accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc
    25141 aaggttttgt actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag
    25201 caacgcattg agcggaacga caaaaaagcc ccccagcacg ccaatcagca tcaacaaggc
    25261 ataggctggc agcagctcgt gttgcaggga gaaaatcggt accaccacgc caatcaaaat
    25321 cccggctggc atacagcgtg acacggtttc cagcgtaacc agcttcgctg ccgcacctgc
    25381 gccaaccacg ataccaatca ctaccatcgc gttgagatag gtcggcgtag cgttatcggt
    25441 aatgcccagc gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc
    25501 ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca ccattgcacc atagcgaggt
    25561 gcaggcattc aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc
    25621 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
    25681 ggccagagcg accaggacat gccagtcagc cagcacacca ccggctacgg aaccgagtaa
    25741 aatcgccgct atggcagaag cttccattaa accgttagct tttactaact tactacccgt
    25801 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacacccac
    25861 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    25921 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    25981 aaacggcgcc aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    26041 ctccggatag aactgcgctt tcagtaacgt cagagtggca aacagtagcg cgttatcgcc
    26101 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    26161 gttaatgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    26221 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    26281 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgcgcg
    26341 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    26401 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    26461 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    26521 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    26581 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    26641 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccagggaccg
    26701 acaacaggcg tgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    26761 tcggtacgtt gatagaaacg acaggcgcgc attcggtcac accgtagcct tcaaggatgc
    26821 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    26881 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    26941 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaag tccggcacaa
    27001 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    27061 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    27121 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    27181 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    27241 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    27301 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    27361 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    27421 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttaccccg gcagtgtagt
    27481 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
    27541 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    27601 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    27661 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    27721 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    27781 cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg catcgccacc tgcgttggag
    27841 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    27901 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    27961 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
    28021 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
    28081 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    28141 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
    28201 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagtacg cgctcgccct
    28261 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    28321 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    28381 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    28441 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    28501 atacctctgg gatcttgatt gtggtctgca cgacgactct tcgccagcga gaaaacgcaa
    28561 aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg ttactgttac agattgatga
    28621 ccggcaaaaa aaaacctgcg catctgcgca ggctggtgta attcatgtgc tcaacacgaa
    28681 gttgacttca cctatcaata cctctgggat caccacttta gcaacctgaa gccaaacgcc
    28741 accagcggtc aatcgcaaca gcgtttcgca aagtgtaacc aaaggtttgc aatctctttt
    28801 tttgtcttac ttatttgcgt ttggctcaca ttcccacgat gaaaacacgc caccccttga
    28861 accaacgggc gttttccgta acactgaaac aatgtaagcg tttacccact aaggtatttt
    28921 catggcgacc ataaaggatg tagcccgact ggcaggcgtt tcagtcgcca ccgtttcccg
    28981 cgtcattaat aattcaccca aagccagcga agcttcccgg ctggctgtgc atagtgcaat
    29041 ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg ctggcgcagc agaccactga
    29101 aacggtcggt ctgatcgttg gtgatgtttc cgatccgttt ttcggtgcaa tggtgaaagc
    29161 ggtcgaacag gtggcttatc acaccggtaa tttcttattg attggcaacg gttaccacaa
    29221 cgaacaaaaa gagcgtcagg ctattgagca actgatccgc catcgctgtg ctgcgttggt
    29281 cgtccatgcc aaaatgatcc cggatgccga tttagcctca ttaatgaaac aaatgcccgg
    29341 tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac cgttgtattg ctcttgacga
    29401 tcgttacggt gcctggctgg caacgcgtca tttaattcag caaggtcata cccgcattgg
    29461 ttatctgtgc tctaaccact ctatttctga cgccgaagat cgtctgcaag ggtattacga
    29521 tgcccttgct gaaagtggta ttccggccaa tgatcggctg gtgacatttg gcgaaccaga
    29581 cgaaagcggc ggcgaacagg caatgaccga gcttttggga cgaggaagaa atttcactgc
    29641 ggtagcctgt tataacgatt caatggcggc gggcgcgatg ggcgttctca atgataatgg
    29701 tattgatgta ccgggtgaga tttcgttaat tggctttgat gatgtgctgg tgtcacgcta
    29761 tgtgcgtccg cgcctgacca ccgtgcgtta cccaatcgta acgatggcga cccaggctgc
    29821 cgaactggct ttggcactgg cggataatcg ccctctcccg gaaattacta atgtatttag
    29881 tccgacgctg gtacgtcgtc attcagtgtc aactccgtcg ctggaggcaa gtcatcatgc
    29941 aaccagcgac taaccgcagt taaagcaatt ccagcgccag taattcttcg atggtctggc
    30001 gacggcgaat caaccgcgcc tgaccattat caaacagaac ttctggtaac agcggacggc
    30061 tattgtagtt ggatgacatt gatgcgccat atgcccctgt atcatgcagt accagataat
    30121 cacctgcctt cacttccggc aaggcgcggg tttcaacatt tcccccttcc tgctgggtaa
    30181 agacatcgcc cgattcacat aacggcccgg cgacgacggt ttccaccgtt ggcgcgtgtt
    30241 ccagagaacg accatcagct gccagggcac tgatatggtg gtaactaccg tacattgccg
    30301 ggcgcatcag atcgttgaac ccggcatcaa ccagcacaaa gtggcggcta cccatttgtt
    30361 tgacgctccg cacctgagta attaatacgc cagactgcgc taccaggaag cgacccggtt
    30421 caatttccag tttcacaggg tggcccaaat ggcgagcgat ttgctcacgc gcggcattcc
    30481 acagaccata ataatgttcg gtatcaaccg cctcttcacc ctgttgataa ggaatagaaa
    30541 gcccaccgcc cgcagaaata gcctgtaaat cctgaccgaa ttcgataacc tgacgcacca
    30601 tagcaccaca cacctgctcc agatgggcat aatcaacgcc agaaccaatg tgcatgtgaa
    30661 tgccgactag ctgcagatga tgacgttgta tcacgtccag tgcggcgggc agatcggtgt
    30721 accagatacc gtgcttgctg ttttcgccac cggtattggt tttttggcta tgtccgtgac
    30781 caaaccccgg attaacgcgc agccataccc gatgccctgg cgaaacctgg cccagttggt
    30841 cgagcgtatc aacagaaccc gcattcaccg gaatttgcaa ttcactgacg cgttcaagcg
    30901 tcgcctgatc gataacatct gccgtaaaaa caatatcatc ggggtgcgtt tgcggattgt
    30961 aacccgccgc caacgcacgc tctatttcgc ctaacgagac ggaatccact ttcacgccct
    31021 gctcacgcat taagcgcaaa atatgaatat tggaacaggc tttctgtgca aagcgcacca
    31081 cttcaaactg tttcagcgct gcaatctgcc gacgaataat ttgcgcatcg tagacccaca
    31141 ccgggcagcc aaattcagcg ggcaaacgca gcagattttc ggcggtgaga tcggtatcgg
    31201 tgctgaacag tgaatgtggc ataacaaact ccagatgagt gcttttttat gattacgcca
    31261 catcataaaa agaataaaaa atatcgattt atgtcgagtc tatgcaaaaa tgatatggat
    31321 taccggattg cgagagagcg ctaatggccg ccgttaactt acgtcatatt gaaatttttc
    31381 atgcggtaat gaccgccgga agcctgactg aggcagcaca cctgctacac acctcacagc
    31441 caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat cgggctgaaa ttgtttgagc
    31501 gcgtacgtgg acgattacat cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac
    31561 gatcctggta cggactggat cgcattgtca gcgccgcaga aagtctgcgc gagtttcgcc
    31621 agggagaact gtctattgcc tgcctgccgg tcttttcgca atctttttta ccgcagctcc
    31681 tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa tatcgtgccc caggaatcac
    31741 cgctacttga agagtggctc tcggcccagc gtcatgattt aggactcact gagacactcc
    31801 atacgcctac gggaacagaa cgtaccgaat tactctcttt agatgaagtg tgtgtgttac
    31861 ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc ggatgatttt cagggtgaga
    31921 attacatcag cctttcccgt actgacagct atcgccagtt gctggatcag ctatttactg
    31981 aacatcaggt taaacgacgc atgatcgtag aaacccacag cgccgcgtca gtctgcgcaa
    32041 tggtacgggc gggggtaggt atttcggtgg ttaacccgct caccgcacta gattatgcgg
    32101 caagcggttt agtggtgcgg cggttcagta ttgcggttcc gttcaccgtc agcctgatcc
    32161 gccccctgca ccgcccgtca tcagcgctgg ttcaggcgtt tagtgggcat ttacaagcgg
    32221 ggttaccgaa actggtcact tctcttgacg ctattttgtc gtcagctacg acagcataaa
    32281 agcgacagca tcctcggcat ggatcgccgc ggtatcaaac acaggcaaaa cactgcgctc
    32341 ttctggcacc agtaaaccaa tttctgtgca gccaaaaatg acgccctgtg cgccctgttc
    32401 tgcaaggcga gcaatcactt gcgcataata agcgcgtgac gcttcggtaa attgccccag
    32461 acacagttct tcaaaaataa tctgattaat tttcgcccgt tcatccgctt caggaataag
    32521 acaattgatg gaaaattgtt ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta
    32581 acgcgtaccc agcagcgcca cacgagtcat tccggccccg gtaattgcac gtccggtggc
    32641 atccgcaatg tgtaagaaag gcagagtgca acgtgactca atggcatccg ccactttatg
    32701 catcgtattg gtacataaca caataccttc tgcgcccgcc cgctgtaagc caagcgccgc
    32761 ctcagccaga atgtccccgg ttttatccca ttccccgcga cgctggcact cttctatttc
    32821 atgaaaatcg acgctatgta gcagcatttg cgcagagtga agcccaccaa gccgctgttt
    32881 aatgccttca tttatcagac gatagtaagg aatggtggat tcccagctca ttcctcccag
    32941 caaaccaatt gttttcattc ctctctcctg atgtgtgtta ccgcaacaag gctgaataag
    33001 gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa aaagggccgg
    33061 atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt ctcgcctgcc
    33121 atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat gagccagaaa
    33181 gtaataccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca
    33241 atgctatcaa gcagtgtcag gaaggccgcg ccgataatca tattcgacac ccagtttgtc
    33301 gtcgtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc agagcacagg
    33361 atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat cgtcatgcca
    33421 acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat caggcaatag
    33481 cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc cggtttacga
    33541 cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga acataaaggt cagtcccacg
    33601 accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc
    33661 ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg caacaacata
    33721 ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca accgccctgt
    33781 tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc cgacgtatcg
    33841 cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc cagccagcgc
    33901 gggctatttg gcaggaagac caccagaata atcagcagaa ctgctggtaa agcaagaccc
    33961 cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa aaacgccagc
    34021 acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg aacgttttca
    34081 cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac agcaatgccc
    34141 agcaccacac gagcggcgat taacatctct acgctggtcg caaaagcgga ccctatagaa
    34201 ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag gcggaacgac
    34261 agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact aaccacccat
    34321 tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc tccggcgatt
    34381 acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga aacaaacata
    34441 ttcatacgcc gcgtatcacg caaagaacgt ggcgttaaag cagattccgt attgatagta
    34501 accatttttt cctgccagca gagagtaaga cattgtgaaa aaatacgtga acaattcaca
    34561 caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat gccgttatgt
    34621 gatggatatt ccaattttaa aattaagttg aattattgag attattatta acctcctaat
    34681 tttaaagcag ataaaattca taaagttcat taattgataa ttgatatgga ttatttcata
    34741 accatgatat ggattatgat gatctagaga tataaaaaac cctgccatgc ggcagggtca
    34801 taaaagtaag aggaatgaat taacgcgcca gccaaccgcc atccacagca atggcataac
    34861 catttacata atctgaagcg ctggaggcaa gaaacactac cggccccatc aggtcactcg
    34921 gcagtcccca acggccagct ggaatgcgat cgagaatttc cgcgctacgt tgttcatctg
    34981 cccgcagttg ttgggtattg ttggtcgcca tgtaacccgg agctatcgca ttaacattaa
    35041 tgttgtgttt agcccattcg tttgccatca atcgcgtcac acccatcacg ccgctttttg
    35101 atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac gcgatattga
    35161 taatcttgcc gccattgcct tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga
    35221 atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg aactcgagag
    35281 cgtcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga ccaaattcag
    35341 ctaccgcgcg atccagcagt ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc
    35401 ttaaaaaacg acgcccctgc gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt
    35461 taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc tggcccagcc
    35521 cagtatcaca accagtgacg accgcaactt taccttggag agaaaatgca cttaaaatca
    35581 taacaacacc ttattcgttt atgcccacaa ctagcgcaaa tctttaacgc ccacatggtc
    35641 catatcatta aagacctggt tttcaccgac catgccccag ataaaggtat aagctttggt
    35701 tccgacgccg gaatggatcg accagctcgg ggagatcacc gcctgctcgt tatgcatcac
    35761 aatgtgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat
    35821 attgaaatag aaataaactt ccatccggcg ctcgtgggtg tgacacggca tggtgttcca
    35881 caagttacct ggagccagct ccgtcagccc catactcaat tgacaggttt ccagtacatc
    35941 cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg aggttatcgc ctaacgtgac
    36001 tggagatact tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca
    36061 attgtaataa aacttcgccg gagtggcggt atcaatactg gcaaagacaa cttcttttgc
    36121 acctttacca acatacaggg cgtcgcggtg accgatttca tagcattgac catcgacagt
    36181 aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaatagct
    36241 tacgccgagt tgtttaccaa cttccccgcc aacggaaacc gttttagtta tcggcataat
    36301 gccgccaaca ataattcggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa
    36361 taccttttca accaaaaatt cattgcgcag cccttgggta tccagcgttt ttgcgtgcgc
    36421 accgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat
    36481 agaaaacgat agtgaaacac tgtttcgttt tctctataaa gggatgatat gcccatgggc
    36541 tagggttttc aattaaaaat aaaacaatgt tttattttta atttaaggat taaaaaaagt
    36601 gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca gaaaatgtat
    36661 gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg
    36721 aggaaatgca gagctactcg tcacgttcaa tggtcaatgc cacgccctgg cccccgccga
    36781 tacaaagcgt tgccagccct ttgcgggcat tacgtttcac catttcatga accagagaaa
    36841 ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga
    36901 cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa
    36961 acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca cgctccaggc
    37021 aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg
    37081 caaatgcgcg gatgcgggcc agcacgggta aattcaacgc tcgtgctttg gcttcgctca
    37141 tcatcattac cgcagctgcg ccatcgttta tggatgatgc attccccgcg gtcacagaac
    37201 cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg tcagtgcgtg
    37261 gctgttcatc ggtatcaaca accaacgtct gaccgttact ttggatcatt accgggacga
    37321 tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg
    37381 cgtaagcatc ctgcaactga cggctgatgc catattcgcg agccagattt tcggcggtga
    37441 caccaatatg ataatcattg aaggcatccc acaacccatc atgcaccaga ctgtcaacca
    37501 actggctatt gccaagctgt gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc
    37561 tcatgttttc ctggccaccg gcgatgacaa tatcagcctc gccacactgt atcgcctgag
    37621 tagccagatg cagtgcttta agaccggaac cgcaaacgtc attaatcgtg attgcagaaa
    37681 cgctgttagg aagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg
    37741 cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt
    37801 ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac
    37861 cacgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa
    37921 cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat cttaattgtt
    37981 taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagtagtcag agtcataact
    38041 ggcacgtaag gtttgcaacc actaacccac caatagatgg gtagataggg cgttaatctc
    38101 ccatacttaa cttggtttat ggtaaattgc cctccatttt gtttaatttg tagatgatac
    38161 gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtgaagttt cgcgctctat
    38221 ggcacagccg ttgatgcagg cacgctattc cttcctattc agttaggttc ggcaggggct
    38281 gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc acataaagcc
    38341 ttatgccagt tcatcctctc atcaaaaaca tcagcaggtg aagggataac gggcgcggta
    38401 acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt
    38461 tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga acagctggca
    38521 aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatt
    38581 ctgaatctca tttttctgat gggacgtcat gccactattc gggtaatggg atttttggta
    38641 ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag ttggcaacct
    38701 gatctattaa caacccaggt agagttcaat cagaataccc ttcaccagat atggatatcg
    38761 attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac
    38821 agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct
    38881 tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca
    38941 tcttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc gctttctatg
    39001 ctgccgaacg ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt agttgcgatg
    39061 tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacaga ggtcgtcaaa
    39121 acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccacgc actatcaatt
    39181 atgttggtat cgctgattac cttcattgtt tgttgcatta acccgaacgc gatttcgatg
    39241 atttacgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgctgtca
    39301 acgtatctca tcccggcgct taaaccctgg cgttccatcg gaaatctgat tacgctgatc
    39361 gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat atccgttgtg gatggttcaa
    39421 aattgctaca attttgaacc attccatttt tcataatatt catttgttat tattccgccc
    39481 tatcacttct ctcgtcatat cctagaatta tcagttagtt aaacacacaa agagatctga
    39541 acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat cttctggtca
    39601 ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc tggaccacgt
    39661 tcgaccatgc aagattaaat cagtgaaaca taatcatatt taataacgag aaatgcattt
    39721 ttaaatatct taatcagaat tatagcaaat acagattaat cgcaattttt cccagcaaaa
    39781 ttacaaaatc aaggtgtatt gacatcatca atcaccaatg aaattatgca attatatacg
    39841 gatagggagg ttcttaacat ggggctttgt agtcgttata aaagtcttac atgcaatagt
    39901 tgctcaatgc attgccaaat aatgccagaa gagtcaccgc gtttgcagta ctgcgcaaac
    39961 tcgtgtt
//
LOCUS       DAJTUN010000023        39743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTUN010000023 DAJTUN010000000
VERSION     DAJTUN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.023"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..156
                     /locus_tag="OO470_001137"
     CDS             <1..156
                     /locus_tag="OO470_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6250454.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            385..975
                     /gene="uidR"
                     /locus_tag="OO470_001138"
     CDS             385..975
                     /gene="uidR"
                     /locus_tag="OO470_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6250455.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1363..3036
                     /gene="uidA"
                     /locus_tag="OO470_001139"
     CDS             1363..3036
                     /gene="uidA"
                     /locus_tag="OO470_001139"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6250456.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3072..4097
                     /gene="uidB"
                     /locus_tag="OO470_001140"
                     /pseudo
     CDS             3072..4097
                     /gene="uidB"
                     /locus_tag="OO470_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4136..5401
                     /gene="uidC"
                     /locus_tag="OO470_001141"
     CDS             4136..5401
                     /gene="uidC"
                     /locus_tag="OO470_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6250457.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5627..7135)
                     /locus_tag="OO470_001142"
     CDS             complement(5627..7135)
                     /locus_tag="OO470_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6250458.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7236..8411)
                     /gene="manA"
                     /locus_tag="OO470_001143"
     CDS             complement(7236..8411)
                     /gene="manA"
                     /locus_tag="OO470_001143"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6250459.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8610..10256
                     /gene="fumB"
                     /locus_tag="OO470_001144"
     CDS             8610..10256
                     /gene="fumB"
                     /locus_tag="OO470_001144"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6250460.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10399..11802
                     /gene="fumC"
                     /locus_tag="OO470_001145"
     CDS             10399..11802
                     /gene="fumC"
                     /locus_tag="OO470_001145"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6250461.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11799..12728)
                     /gene="tus"
                     /locus_tag="OO470_001146"
     CDS             complement(11799..12728)
                     /gene="tus"
                     /locus_tag="OO470_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6250462.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="OO470_001147"
     CDS             complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="OO470_001147"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6250463.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14284..15603)
                     /locus_tag="OO470_001148"
     CDS             complement(14284..15603)
                     /locus_tag="OO470_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6250464.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="OO470_001149"
     CDS             complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="OO470_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6250465.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16700..17035
                     /locus_tag="OO470_001150"
     CDS             16700..17035
                     /locus_tag="OO470_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6250466.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17032..17754)
                     /gene="folM"
                     /locus_tag="OO470_001151"
     CDS             complement(17032..17754)
                     /gene="folM"
                     /locus_tag="OO470_001151"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6250467.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17791..19173)
                     /locus_tag="OO470_001152"
     CDS             complement(17791..19173)
                     /locus_tag="OO470_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6250468.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19359..20303)
                     /locus_tag="OO470_001153"
     CDS             complement(19359..20303)
                     /locus_tag="OO470_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6250469.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20827..22359
                     /gene="pntA"
                     /locus_tag="OO470_001154"
     CDS             20827..22359
                     /gene="pntA"
                     /locus_tag="OO470_001154"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6250470.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22370..23758
                     /gene="pntB"
                     /locus_tag="OO470_001155"
     CDS             22370..23758
                     /gene="pntB"
                     /locus_tag="OO470_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6250471.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="OO470_001156"
     CDS             complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="OO470_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6250472.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25228..25593
                     /gene="mdtJ"
                     /locus_tag="OO470_001157"
     CDS             25228..25593
                     /gene="mdtJ"
                     /locus_tag="OO470_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6250473.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25580..25909
                     /gene="mdtI"
                     /locus_tag="OO470_001158"
     CDS             25580..25909
                     /gene="mdtI"
                     /locus_tag="OO470_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6250474.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25948..26769)
                     /locus_tag="OO470_001159"
     CDS             complement(25948..26769)
                     /locus_tag="OO470_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6250475.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26869..26952)
                     /locus_tag="OO470_001160"
     CDS             complement(26869..26952)
                     /locus_tag="OO470_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250476.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27045..27380)
                     /locus_tag="OO470_001161"
     CDS             complement(27045..27380)
                     /locus_tag="OO470_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6250477.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27948..28205)
                     /locus_tag="OO470_001162"
     CDS             complement(27948..28205)
                     /locus_tag="OO470_001162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250478.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28221..29030)
                     /locus_tag="OO470_001163"
     CDS             complement(28221..29030)
                     /locus_tag="OO470_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250479.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29137..30039
                     /locus_tag="OO470_001164"
     CDS             29137..30039
                     /locus_tag="OO470_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6250480.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30174..31394
                     /gene="mlc"
                     /locus_tag="OO470_001165"
                     /gene_synonym="dgsA"
     CDS             30174..31394
                     /gene="mlc"
                     /locus_tag="OO470_001165"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6250481.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31519..32214
                     /gene="bioD"
                     /locus_tag="OO470_001166"
     CDS             31519..32214
                     /gene="bioD"
                     /locus_tag="OO470_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6250482.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32167..33458)
                     /gene="clcB"
                     /locus_tag="OO470_001167"
                     /pseudo
     CDS             complement(32167..33458)
                     /gene="clcB"
                     /locus_tag="OO470_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="OO470_001168"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="OO470_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6250483.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /locus_tag="OO470_001169"
     CDS             complement(34274..35128)
                     /locus_tag="OO470_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6250484.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="OO470_001170"
     CDS             complement(35130..35747)
                     /gene="dmsB"
                     /locus_tag="OO470_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6250485.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..37796)
                     /locus_tag="OO470_001171"
                     /pseudo
     CDS             complement(35758..37796)
                     /locus_tag="OO470_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37857..38966)
                     /locus_tag="OO470_001172"
     CDS             complement(37857..38966)
                     /locus_tag="OO470_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6250486.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38949..>39743
                     /locus_tag="OO470_001173"
     CDS             38949..>39743
                     /locus_tag="OO470_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6250487.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT        10118 a         9634 c        10173 g         9818 t
ORIGIN      
        1 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt
       61 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      121 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt
      181 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      241 gcagccaaat cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg
      301 taccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      361 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      421 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      481 tcgatgaaag ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc
      541 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct
      601 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      661 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
      721 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
      781 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
      841 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
      901 gaagacctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata
      961 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1021 tcgtaatttc tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata
     1081 ttcaccgtag ccatatgtca tgagagttta tcgttcccaa tactctcgaa cgaacgttcg
     1141 attgcttatt ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg
     1201 ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1261 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1321 ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa
     1381 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1441 aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca
     1501 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1561 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1621 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1681 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     1741 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     1801 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     1861 tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     1921 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     1981 gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     2041 ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     2101 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2161 ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     2221 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2281 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2341 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     2401 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2461 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2521 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2581 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     2641 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     2701 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     2761 cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     2821 aaagaactgc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     2881 gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     2941 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3001 caggtatgga atttcgccga ttttgcgacc tcgtaaggca tattgcgcgt tggcggtaac
     3061 aattgaccta catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac
     3121 ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga
     3181 ttgccgcttc attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact
     3241 ccagcccgga agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg
     3301 gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg
     3361 cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt
     3421 tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt
     3481 tctacgtgcg ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa
     3541 acctggttgg tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta
     3601 aaaagaatac cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct
     3661 gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc
     3721 agggcgttac tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat
     3781 acctgaccgg cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat
     3841 gcggtcaggc aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg
     3901 ccaatcaggt gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac
     3961 cttgcggatt tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa
     4021 aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca
     4081 gcgatatcac taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag
     4141 aaaaatagtg gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta
     4201 cgcggcgcaa ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg
     4261 cagggctgat aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg
     4321 attgctcgat ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta
     4381 ttatgtttat aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga
     4441 taaaagtttt ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct
     4501 taaattaggt cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt
     4561 aatggttggc agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg
     4621 ggctttaacg ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac
     4681 tgattcaaca accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga
     4741 taaacatcgc gcacgttctt ttttagccgc cagttggcat gatgatacca gtcgctattc
     4801 tctgggggca tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag
     4861 cataccgctc gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct
     4921 cgaaggttta agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt
     4981 gacctggaat gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat
     5041 gaatggtgcc gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa
     5101 ccgtgaagga atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac
     5161 gctgacattt gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat
     5221 tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg
     5281 gatgaatttc tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg
     5341 cgatcgcctg aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta
     5401 agaaataacg ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt
     5461 tttatgccgg atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt
     5521 gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc
     5581 agatgaaaac gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa
     5641 ttgccggtac agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta
     5701 acgacatttt ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga
     5761 tggtattgtc ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa
     5821 cttgctgttt cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct
     5881 gagtcatcaa ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa
     5941 ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt
     6001 tcaggaacag cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg
     6061 cgatagtaat caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg
     6121 tcactttctc ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct
     6181 gagtttgcgc gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga
     6241 cttttaaagt cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg
     6301 agtaatccag ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga
     6361 tctccatgcc ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca
     6421 gtgacagttt ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg
     6481 ttttcagatt attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc
     6541 gaccactttg cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact
     6601 ggaactcgcc gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga
     6661 gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg
     6721 tttcaccttt tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt
     6781 gaattgatgc catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac
     6841 catgatcaac cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt
     6901 tcccggcaat gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt
     6961 tttgataact cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct
     7021 gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg
     7081 tccagactac gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga
     7141 ttatccataa aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa
     7201 gcttagcaag agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct
     7261 aaacggccgt ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca
     7321 cccggtttaa gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag
     7381 aacaaaatgg cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc
     7441 gagaaggcaa aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc
     7501 tgggtcaaca actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga
     7561 atatcaatgt atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact
     7621 tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg
     7681 ccaggattca atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg
     7741 taaaattcag aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc
     7801 gattttaaaa tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg
     7861 aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt
     7921 gcaccggcga ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc
     7981 gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta
     8041 cgctcggcgg catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa
     8101 ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac
     8161 aggaaaggca gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca
     8221 ctctcaatca catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg
     8281 cttttcggat gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata
     8341 ccataaagtt cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg
     8401 agtttttgca tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga
     8461 atcctacggg aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa
     8521 aactgtgtag gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat
     8581 aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc
     8641 cttttccact caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg
     8701 tatctgaatt tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg
     8761 cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg
     8821 ccgacattct gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc
     8881 tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg
     8941 cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc
     9001 tggcgcgcgg tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc
     9061 cgctggatat gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt
     9121 atgccgttga tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa
     9181 acaagacgta tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt
     9241 acctggttga gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt
     9301 tcgttattgg tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga
     9361 aatactatga tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg
     9421 aactggaaaa agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg
     9481 gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc
     9541 cggttggtat gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc
     9601 aggggatctg gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc
     9661 gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc
     9721 tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg
     9781 ttggtcgtga tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc
     9841 cgcaatacat taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt
     9901 atgcctccgg ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac
     9961 tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga
    10021 cggatgcctg taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg
    10081 tattggcgca gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg
    10141 aagccatctg gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag
    10201 gaaatgactt cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag
    10261 agccgccctt cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat
    10321 agtaaataat gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa
    10381 tcaggtgagg agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt
    10441 gatgtcccgg cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc
    10501 atttcgacgg agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca
    10561 gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg
    10621 caggcggcgg atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg
    10681 cagaccggct ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc
    10741 agtgaattac tcggcggtgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg
    10801 aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg
    10861 gcactgcgca agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa
    10921 tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg
    10981 ctaacactag ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat
    11041 atcgaataca gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact
    11101 ggactaaata cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc
    11161 tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg
    11221 gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat
    11281 gtccgctggc tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat
    11341 gagccgggca gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc
    11401 atgctctgct gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt
    11461 aactttgaac tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc
    11521 ttgctggcag atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat
    11581 cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc
    11641 cacattggtt atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc
    11701 ttaaaagctg cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta
    11761 cggccagaac agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc
    11821 agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg
    11881 tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta
    11941 atcagtgcaa tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac
    12001 cagacgcggg caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt
    12061 ggcagggcag cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc
    12121 cacggtgcga cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca
    12181 cgatgcaaat tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg
    12241 tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc
    12301 acccattcaa aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac
    12361 gtggtcttga gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta
    12421 tcgacctggt aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg
    12481 gactgttgtt gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg
    12541 ccgagatgtt gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc
    12601 tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat
    12661 gtagccagct cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa
    12721 cgcgccataa tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg
    12781 gcttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac
    12841 ggccagctaa acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg
    12901 cccattgcca gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag
    12961 cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg
    13021 gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg
    13081 ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg
    13141 cgctccatta agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt
    13201 accgttttat cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc
    13261 aggtctggtt cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc
    13321 agctcttcaa ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg
    13381 gcgctcaggt tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt
    13441 tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc
    13501 gccatctggt taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata
    13561 cgttcattga gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc
    13621 tgccagtgcg gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg
    13681 atcagggcga tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacca
    13741 actgccagca cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg
    13801 gcgacaattt cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc
    13861 agtggctcga cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc
    13921 cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc
    13981 aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc
    14041 agcagagaca tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt
    14101 ttcattggca tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg
    14161 taaaccgcca actggataag accttgtttg gaagataatg tccggaacag acggatgtat
    14221 ccccggtgta ttcatattca gtaaattcaa ttttacaccg gggcttgttt attatgctaa
    14281 ggtttaattt aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc
    14341 tgatattaca ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac
    14401 gatcggctct atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag
    14461 gacaatcagc gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag
    14521 cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt
    14581 tggagcaatc atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat
    14641 gctggaacca aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa
    14701 tgcagaaata gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag
    14761 tacttgccag acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc
    14821 cgagtgaaca ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa
    14881 attggtaaac tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc
    14941 ataaggcgtc agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt
    15001 gatccccgcg ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc
    15061 aataccagca agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt
    15121 cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg agtcgcggta aagataccgc
    15181 agctgttttc gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc
    15241 atcaaggcca attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca
    15301 caacatacag gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt
    15361 attaatcgct gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt
    15421 aacataaccg ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg
    15481 aatgaaatgc agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt
    15541 ggaaagggat tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag
    15601 cacaatatat gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa
    15661 atgccggaaa gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta
    15721 tagagaaatt ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc
    15781 ttattaattg aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat
    15841 tgtttattgt cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    15901 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    15961 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    16021 tcgcggtcca tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct
    16081 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt
    16141 aacgtgccga aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc
    16201 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    16261 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    16321 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    16381 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt
    16441 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    16501 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    16561 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    16621 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    16681 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    16741 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    16801 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc
    16861 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    16921 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    16981 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    17041 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta
    17101 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    17161 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    17221 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    17281 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    17341 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    17401 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    17461 cagtggcgca cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag
    17521 accatgggtg gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    17581 atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt
    17641 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc
    17701 gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat
    17761 ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac
    17821 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    17881 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga
    17941 taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc
    18001 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    18061 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    18121 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc
    18181 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    18241 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    18301 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    18361 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga
    18421 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    18481 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    18541 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag
    18601 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    18661 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    18721 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    18781 tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa
    18841 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca
    18901 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    18961 gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    19021 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    19081 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    19141 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    19201 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    19261 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    19321 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    19381 acggtcaggt tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct
    19441 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    19501 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    19561 actttagtgc catccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca
    19621 aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact
    19681 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    19741 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    19801 atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca
    19861 cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    19921 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    19981 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    20041 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    20101 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    20161 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    20221 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    20281 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    20341 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    20401 catttttcat atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct
    20461 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    20521 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    20581 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    20641 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    20701 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    20761 tgagcagctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    20821 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    20881 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    20941 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    21001 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    21061 gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    21121 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg
    21181 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt
    21241 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact
    21301 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg
    21361 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    21421 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    21481 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    21541 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    21601 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    21661 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt
    21721 gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    21781 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    21841 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    21901 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    21961 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    22021 ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc
    22081 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    22141 tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac
    22201 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt
    22261 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    22321 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    22381 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    22441 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    22501 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    22561 ggtcattggt ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc
    22621 agaactggtg gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    22681 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    22741 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg
    22801 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    22861 cctggcggct ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt
    22921 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    22981 tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta
    23041 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    23101 cggtgcgctg gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa
    23161 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    23221 tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    23281 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    23341 tcctgtcgct gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat
    23401 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    23461 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    23521 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    23581 tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    23641 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    23701 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    23761 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    23821 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa
    23881 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    23941 atgtcgaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    24001 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    24061 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    24121 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    24181 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    24241 cactgtcaat cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    24301 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    24361 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    24421 ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt
    24481 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    24541 tccacgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    24601 cgatgattgt catcaaaatc gacactgcca gtacacgtgg cacacgccag cggaccatat
    24661 gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga
    24721 tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga
    24781 tttttaggcc attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt
    24841 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    24901 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    24961 accgaggaca attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta
    25021 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    25081 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    25141 ttgccctatg cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    25201 aaatagttct cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct
    25261 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    25321 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    25381 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    25441 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    25501 ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    25561 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    25621 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    25681 caaaatattt ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca
    25741 ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc
    25801 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    25861 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    25921 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    25981 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    26041 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    26101 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    26161 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg
    26221 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    26281 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca
    26341 cggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    26401 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    26461 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    26521 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    26581 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    26641 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    26701 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    26761 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    26821 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    26881 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    26941 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    27001 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    27061 gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc
    27121 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    27181 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc
    27241 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    27301 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    27361 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    27421 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    27481 ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat
    27541 agtagtcttg atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc
    27601 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    27661 cgctagatgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    27721 tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag
    27781 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    27841 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    27901 gcaatactcg accccagata gtaactgaac agatacagcg aggaggccta gcctttagcg
    27961 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    28021 gagaagagta acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa
    28081 ccaaacggca taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc
    28141 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    28201 accacggcct gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac
    28261 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    28321 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa
    28381 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    28441 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    28501 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    28561 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    28621 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    28681 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    28741 gcatcggata gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac
    28801 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    28861 ggctgcacac aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg
    28921 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    28981 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    29041 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc
    29101 taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg
    29161 cgttactttg ttgctgttgc ggaagagctg cattttgggc gcgcggctgc ccgcctgaat
    29221 atttcgcaac cgccgctaag tcagcagatt caggcactgg agcaacaaat cggtgcccga
    29281 ctgctggcac gaactaatcg cagtgtattg ctgacggcag caggaaaaca gtttcttgca
    29341 gatagtcggc aaatcctgtc tatggtggat gacgctgccg ctcgcgctga aagactgcat
    29401 cagggtgaag cgggggagtt gcgtattggt tttacttcgt ccgctccttt tattcgggcg
    29461 gtgtccgata cgttatcgct gtttcgccgt gattatcctg atgtccattt acaaacccgc
    29521 gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg
    29581 ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt
    29641 atggcgatga tcccgtacga tcatcccctg gcaaataatc cgagtgtaac actggctgaa
    29701 ctggctgaac tggcgaaaga accctttgtc ttttttgatc cgcacgtcgg gactgggctg
    29761 tatgacgata ttctcggact gatgcgacgt taccatttga cgcccgtcat cactcaggag
    29821 gtgggcgagg caatgaccat catcggtctg gtttccgccg gtctgggtgt ttcaattttg
    29881 cctgcgtcat ttaaacgtgt tcagctcaac gaaatgcgct gggtgccgat tgctgaagag
    29941 gatgcggttt ctgaaatgtg gttggtctgg ccgaaacatc atgaacaaag tccagctgcg
    30001 cgtaactttc gtattcatct gctgaatgct ctcaggtgag ggaaatttca gcgaaaaagc
    30061 ccgaaaaatg tgctgttaat cacatgccta agtaaaaatt tgacgacacg tattgaagtg
    30121 cttcaccata gcctacagat tatttcggag cgcgaaaata tagggagtat gcggtggttg
    30181 ctgaaaacca gcctgggcac attgatcaaa taaagcagac caacgcgggc gcggtttatc
    30241 gcctgattga tcagcttggt ccagtctcgc gtatcgatct ttcccgtctg gcgcagctgg
    30301 ctcctgccag tatcactaaa attgtccgtg agatgctcga agcacacctg gtgcgagagc
    30361 tggaaatcaa agaagcgggg aaccgtggcc gtccggcggt ggggctggtg gttgaaactg
    30421 aagcctggca ctatctttct ctgcgcatta gtcgcgggga gattttcctt gctctgcgcg
    30481 atctgagcag caaactggtg gtggaagagt cgcaggaact ggcgttaaaa gatgacttgc
    30541 cattgctgga tcgtatcatt tcccatatcg atcagttttt tatccgccac cagaaaaaac
    30601 ttgagcgtct aacttcgatt gccataaccc tgccgggaat tattgatacg gaaaatggta
    30661 ttgtacatcg catgccgttc tacgaggatg taaaagagat gccgctcggc gaggcgctgg
    30721 agcagcatac cggcgtaccg gtttatattc agcatgatat cagcgcatgg acgatggcag
    30781 aggccttgtt tggtgcctca cgcggggcgc gcgatgtgat tcaggtggtt atcgatcaca
    30841 acgtgggggt gggcgtcatt accgatggtc atctgctaca cgcaggcagc agtagtctcg
    30901 tggaaatagg ccacacacag gtcgacccat atggtaaacg ctgttattgc gggaaccacg
    30961 gctgcctcga aaccatcgcc agtgtggaca gtattcttga gctggcacag ctgcgtctca
    31021 atcaatccat gagctcgatg ttacatgggc aaccgttaac cgtggactca ttgtgtcagg
    31081 cggcattgcg cggcgatcta ctggcaaaag acatcattat cggggtgggc gcgcatgtag
    31141 ggcgcattct tgccatcatg gtgaatttat ttaacccaca aaaaatactg attggctcac
    31201 cgttaagtaa agcggcagat atcctcttcc cggtcatctc agacagcatc cgtcagcagg
    31261 cccttcctgc gtatagtcag cacattagcg ttgagagtac tcagttttct aaccagggca
    31321 cgatggcagg cgctgcactg gtaaaagacg cgatgtataa cggttctttg ttgattcgtc
    31381 tgttgcaggg ttaacatttt ttaactgttc taccaaaatt tgcgctatct caatttgggc
    31441 caggaaagca taacttagac tttcaaggtt aattattttc ctagtttata tttgtgaagc
    31501 ataacggtgg agttagtgat gctgaagcgt ttctttatta ccggtacaga cacttctgta
    31561 gggaaaacgg tggtttcccg cgcattgcta caagcgttag cctcccaggg aaaaagggtt
    31621 gcgggatata aacccgtagc gaaggggagc aaagagacac ccgaagggct gcgtaataaa
    31681 gatgccttgg tgttgcagag tgtttcaacc atcgaactgc cttatgaagc agttaatcct
    31741 atcgcgttaa gcgaagaaga aagtagcgtg gcgcacagtt gcccaatcaa ttacaccctc
    31801 atttcaaacg gcctggcaaa cctgaccgat aaagtcgatc atgtcgtggt agaagggact
    31861 ggcggctggc gcagtctgat gaatgatttg cgtccactct ctgaatgggt agtgcaggaa
    31921 caactgccgg tgttgatggt tgtcggtatt caggaaggtt gcattaacca tgcactgcta
    31981 acagctcagg cgatcgccaa cgacgggctg ccgctcattg gctgggtggc taaccgaatc
    32041 aacccaggac tggcgcatta tgcggaaatc attgatgtgc tgggtaaaaa acttccggca
    32101 ccgctcattg gtgaactgcc ttatctgccg cgcgccgaac agcgtgaact ggggcaatac
    32161 atccgcttag ctatgctgcg cagtgtgctg gcggtagata gagtcacggt gtaacgtccg
    32221 cgaaattacc gacgcaatta cgcaggcaat caataaaccg gggagtagct gatactcccc
    32281 ggtcatttca catatcatca acgtcgacat aatcggcgcg tgcgtggttg ccgccaacag
    32341 tgtcgccatc ccggtcaatc cgagtaaaag cgtaatttct tcgccatcag ggaaccataa
    32401 tcccaggcta cgaccataca acatgccaat ggcaagaccg ataaatagcg tcggtgtaaa
    32461 gacaccaccg ggtgcgccgg aaccgctact cgccagcacg gcacacagtt tacaaaggaa
    32521 gatcccggca atgatcatta acagtggtgg ggcggttaaa aaggattgta cggtgctata
    32581 gccgttgccc cacactgcag gtgtgaacag ggaaagcaga cccacgatca acccgactag
    32641 tgccagttgc cagggcggcg caagtttgag actcacaaat ccacgatgac aggcgttcat
    32701 taacgttaac aacagtggtc cgcacagacc tgccagcaca cctgtactga taatcagcgc
    32761 atagtcacga gcctgaaccg tcactgagag ttgtacgttg tagagtaacg cgtcgctatg
    32821 attaatcaga ttgctaacca gccatgccac gatggcggaa ataatcaccg ggccgagaga
    32881 ggccaacatc atagtgccaa acagcacttc ggctataaat aaactgccag caagcggggc
    32941 acgataggcc gcagccattc ccgccgcggc cccacaggcg atccataatt tccattcctg
    33001 gcgtggcgta aaacgttggg caaaacagga ggcggcaagg gcagctaaaa gaatcatcgc
    33061 accttcgcga ccaattgcac tgccgctggt tactaccagc agcgaggcaa gcgatttaac
    33121 caggcttgct gcgtaatcga actgtccatc ggtttgcaac gcttccatgt aatcggtcgg
    33181 cgcatgaggg cgttgttggg taatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtacgcgct ttggtttggc gacttcactc
    36121 agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg ggcgaggatc
    36181 acatagccca tattccctgc agattcatga gagataaggt cttcttgctc tgttggcatc
    36241 aggtcaggca acaggatatc gcaatactta gccgaggccg tcatgaagtg gtcaatgcca
    36301 acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt gatgtcgcca
    36361 tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg gacatccagt
    36421 ttttcttttc cacgaacacc atcacgggtc gcggtcattt tcgtaccatg gtcgatggca
    36481 tctgtccatg taaaaacgga aatctgcgtt ttaacaggat tctcgagcat cgggaaccat
    36541 tctaccccca gatcccagct accttcgcgt acacctgagt tgccgccgtt tatgccgacg
    36601 ttaccggtaa gaacggaaag catggcaata gcgcgggacg tttgttcgcc gttggagtgt
    36661 cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat ctctcgtgcc
    36721 aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc cggcgtttta
    36781 gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt acgtggtgcg
    36841 ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa tggctgatcg
    36901 accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc atcggtgcca
    36961 gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata gcgtggatcg
    37021 atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta agtgacacca
    37081 ccgccgctca tccgcgtttc tgccgggtga tttccgaaca taacgaccag tttcgtattg
    37141 gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat tgcggcactg
    37201 atctgtgcgg tactgtagct gccatagcga ctgagaaaac ccccgcaaga gttcatcaga
    37261 cggtacggga cgttcgagtt ggtgatattt ccgccatcta cgcctgttcc gtacaggaca
    37321 tgtacagcct cattgccgta atctttcagg atccgccgaa gattatcact gatggtatcc
    37381 agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgctcgcc gacgcgcttc
    37441 atgggatatt tcaacctgtc aggatgattc atccgtcggc ggatagagcg cccgcgtaaa
    37501 catgcccgaa cctgatgatt accgtaaacg tcgtcacctg tcgtatcaga ctccacccag
    37561 tacacggtgt catctttcac atgcaaacgt agcagacagc ggctcccgca gttaacggtg
    37621 caggaactcc agaccgtttt ctcttctacc ggagcctctg ccgcccggac catttgggaa
    37681 aatggcagag tgaaagcact gcttgccagc gcgagactgc caagtgcgga ggttttcatc
    37741 agacttctat ggctgatttc agccttcatg agcgcctctg tggtatggat tttcatcatt
    37801 actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt agtgggttat
    37861 attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag tggtgaaggg
    37921 cgcagagtgg ttagcgtatt cacacatccg ccatggtcga ttttatcgcc agacatattg
    37981 ggctcgtgcc aggctccctg gcccatagcg ctaactccag ggagaatgcg tggtgttact
    38041 ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc gccgttggca
    38101 atcccacgtt tctgtgcatc tatagggttg atccacacct cctgacggca ggctgccttc
    38161 aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa accaaacagt
    38221 tgcagtggga aggttctacg ttcaggggag tcccagcctt caaaggttga ggcataaact
    38281 ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat ttccgccagc
    38341 ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc ctcggggtcg
    38401 tcacgaaatg ctttgtaggc gacaaaatgg ccattgggat ctttacgctt atagataccc
    38461 atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat tttggcgtac
    38521 agatgttgta accattgttc ctgcgtgcga ccttctgtga acttttgata gacgtcaggt
    38581 ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttacgttc gaatttttcg
    38641 ctggtgactg gctggaggaa aatgagatat cccatgttac cggcgtagtc gttaggaata
    38701 atatcttcct gctcaacggt catcaggtct gccagcagaa tgtcggcata ttttgccgat
    38761 gaggtcataa agttttcgat gaccacaatc atttcgcatt tcgattcgtc ctgcagaatt
    38821 tcatgcgttt tgttgatgtc agaatgctga ttaacgaggg tatttcccgc gtagttccag
    38881 atggactcta ctgtcagacg gatggacgga cttgaagtag ttgactcaac gctacttaag
    38941 gtcctgatat gactgacatt aaccatgacg atactgcata tccatccagt tctttctctg
    39001 ggtctcccta tctgtatcag caagtctaca cctcaagtcc tgacacaagg ctgacacaaa
    39061 tcaaggacct tgctacgcca cagctagtcc ggagaaccaa cgggcgttac actgtccgct
    39121 tcagacttaa aggagctacc agcccgtttc tatcagtctc aacccgcagc acagaccgga
    39181 ggaccgctac aatgcgtcag agagagctcg cagccacagc taaggcattc ctactggaca
    39241 atcctgaagt cacactacag gagcttacag cgcacatacg gtccatggct gagcagttcc
    39301 tgactgatgc cagtgatgac tactggaatg gcgtagaggt ggcgacactg gttgacgaga
    39361 agtccaacct cagggaactt gcagcgacaa gagcgttgag tgtggaccag cagaggggca
    39421 ttaagttagc gctggaagtc ctgaccgcag cacagcagag agtcgacact ggtgatacct
    39481 ctggtctgat taagctgatt gatggtggcg taagccagct tactgatgat aataaccata
    39541 ctgattactc aactataggc gactctacgt cgattctgaa gaatgagcag ggggatagac
    39601 cagcagtttt cacacaggaa cgtcagttac tcactccaga gtcacgagaa tcatctgtac
    39661 tttttcagga attggtgaca tcactgctgg ctgagaaggt ccagacactg aaacaatcca
    39721 gctttaagga cttgtcagcg tcc
//
LOCUS       DAJTUN010000024        38024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTUN010000024 DAJTUN010000000
VERSION     DAJTUN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.024"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..228)
                     /gene="yefM"
                     /locus_tag="OO470_001174"
     CDS             complement(<1..228)
                     /gene="yefM"
                     /locus_tag="OO470_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6250488.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKG"
     gene            511..552
                     /gene="hisL"
                     /locus_tag="OO470_001175"
     CDS             511..552
                     /gene="hisL"
                     /locus_tag="OO470_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6250489.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    528..651
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            698..1597
                     /gene="hisG"
                     /locus_tag="OO470_001176"
     CDS             698..1597
                     /gene="hisG"
                     /locus_tag="OO470_001176"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6250490.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1603..2907
                     /gene="hisD"
                     /locus_tag="OO470_001177"
     CDS             1603..2907
                     /gene="hisD"
                     /locus_tag="OO470_001177"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6250491.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2904..3974
                     /gene="hisC"
                     /locus_tag="OO470_001178"
     CDS             2904..3974
                     /gene="hisC"
                     /locus_tag="OO470_001178"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6250492.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3974..5041
                     /gene="hisB"
                     /locus_tag="OO470_001179"
     CDS             3974..5041
                     /gene="hisB"
                     /locus_tag="OO470_001179"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6250493.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5041..5631
                     /gene="hisH"
                     /locus_tag="OO470_001180"
     CDS             5041..5631
                     /gene="hisH"
                     /locus_tag="OO470_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6250494.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5631..6368
                     /gene="hisA"
                     /locus_tag="OO470_001181"
     CDS             5631..6368
                     /gene="hisA"
                     /locus_tag="OO470_001181"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6250495.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6350..7126
                     /gene="hisF"
                     /locus_tag="OO470_001182"
     CDS             6350..7126
                     /gene="hisF"
                     /locus_tag="OO470_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6250496.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7120..7731
                     /locus_tag="OO470_001183"
     CDS             7120..7731
                     /locus_tag="OO470_001183"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6250497.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7917..8894)
                     /gene="wzzB"
                     /locus_tag="OO470_001184"
     CDS             complement(7917..8894)
                     /gene="wzzB"
                     /locus_tag="OO470_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6250498.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9040..10160)
                     /gene="ugd"
                     /locus_tag="OO470_001185"
                     /pseudo
     CDS             complement(9040..10160)
                     /gene="ugd"
                     /locus_tag="OO470_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10410..11816)
                     /gene="gndA"
                     /locus_tag="OO470_001186"
     CDS             complement(10410..11816)
                     /gene="gndA"
                     /locus_tag="OO470_001186"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6250499.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11925..12284)
                     /locus_tag="OO470_001187"
     CDS             complement(11925..12284)
                     /locus_tag="OO470_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250500.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12281..12787)
                     /locus_tag="OO470_001188"
     CDS             complement(12281..12787)
                     /locus_tag="OO470_001188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250501.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12933..13496)
                     /locus_tag="OO470_001189"
     CDS             complement(12933..13496)
                     /locus_tag="OO470_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250502.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13703..14641)
                     /locus_tag="OO470_001190"
                     /pseudo
     CDS             complement(13703..14641)
                     /locus_tag="OO470_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14704..15078)
                     /locus_tag="OO470_001191"
     CDS             complement(14704..15078)
                     /locus_tag="OO470_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6250503.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15082..16230)
                     /gene="wzy"
                     /locus_tag="OO470_001192"
     CDS             complement(15082..16230)
                     /gene="wzy"
                     /locus_tag="OO470_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6250504.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16250..17155)
                     /gene="rfbG"
                     /locus_tag="OO470_001193"
     CDS             complement(16250..17155)
                     /gene="rfbG"
                     /locus_tag="OO470_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6250505.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17148..18038)
                     /locus_tag="OO470_001194"
     CDS             complement(17148..18038)
                     /locus_tag="OO470_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6250506.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18068..19324)
                     /gene="wzx"
                     /locus_tag="OO470_001195"
     CDS             complement(18068..19324)
                     /gene="wzx"
                     /locus_tag="OO470_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6250507.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19340..19885)
                     /gene="rfbC"
                     /locus_tag="OO470_001196"
     CDS             complement(19340..19885)
                     /gene="rfbC"
                     /locus_tag="OO470_001196"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6250508.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19890..20768)
                     /gene="rfbA"
                     /locus_tag="OO470_001197"
     CDS             complement(19890..20768)
                     /gene="rfbA"
                     /locus_tag="OO470_001197"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6250509.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20827..21726)
                     /gene="rfbD"
                     /locus_tag="OO470_001198"
     CDS             complement(20827..21726)
                     /gene="rfbD"
                     /locus_tag="OO470_001198"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6250510.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21726..22811)
                     /gene="rfbB"
                     /locus_tag="OO470_001199"
     CDS             complement(21726..22811)
                     /gene="rfbB"
                     /locus_tag="OO470_001199"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6250511.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23184..24077)
                     /gene="galF"
                     /locus_tag="OO470_001200"
     CDS             complement(23184..24077)
                     /gene="galF"
                     /locus_tag="OO470_001200"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6250512.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24252..25646)
                     /gene="wcaM"
                     /locus_tag="OO470_001201"
     CDS             complement(24252..25646)
                     /gene="wcaM"
                     /locus_tag="OO470_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6250513.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25657..26877)
                     /gene="wcaL"
                     /locus_tag="OO470_001202"
     CDS             complement(25657..26877)
                     /gene="wcaL"
                     /locus_tag="OO470_001202"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6250514.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26874..28154)
                     /gene="wcaK"
                     /locus_tag="OO470_001203"
     CDS             complement(26874..28154)
                     /gene="wcaK"
                     /locus_tag="OO470_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6250515.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28334..29812)
                     /gene="wzxC"
                     /locus_tag="OO470_001204"
     CDS             complement(28334..29812)
                     /gene="wzxC"
                     /locus_tag="OO470_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6250516.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29814..31208)
                     /gene="wcaJ"
                     /locus_tag="OO470_001205"
     CDS             complement(29814..31208)
                     /gene="wcaJ"
                     /locus_tag="OO470_001205"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6250517.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31263..32633)
                     /gene="cpsG"
                     /locus_tag="OO470_001206"
                     /gene_synonym="manB"
     CDS             complement(31263..32633)
                     /gene="cpsG"
                     /locus_tag="OO470_001206"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6250518.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32826..34262)
                     /gene="cpsB"
                     /locus_tag="OO470_001207"
     CDS             complement(32826..34262)
                     /gene="cpsB"
                     /locus_tag="OO470_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6250519.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34265..35488)
                     /gene="wcaI"
                     /locus_tag="OO470_001208"
     CDS             complement(34265..35488)
                     /gene="wcaI"
                     /locus_tag="OO470_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6250520.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35485..35964)
                     /gene="gmm"
                     /locus_tag="OO470_001209"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35485..35964)
                     /gene="gmm"
                     /locus_tag="OO470_001209"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6250521.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35964..36922)
                     /gene="fcl"
                     /locus_tag="OO470_001210"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35964..36922)
                     /gene="fcl"
                     /locus_tag="OO470_001210"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36925..>38024)
                     /gene="gmd"
                     /locus_tag="OO470_001211"
     CDS             complement(36925..>38024)
                     /gene="gmd"
                     /locus_tag="OO470_001211"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6250522.1"
                     /translation="GVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPH
                     TCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGT
                     LRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWIT
                     VNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRD
                     WGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEK
                     GIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREM
                     VSEMVANDLEAAKKHSLLKSHGYDVAIALES"
BASE COUNT        10171 a         9525 c         8563 g         9765 t
ORIGIN      
        1 tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt tagcggggga
       61 acgcagtaga taagccgtct cttccagcga attgtattct tcgagtgaca tcagaacaca
      121 agcctctcca ttctgacgag taatgaggat cggggcatga tcttcaacgg ctttcatcat
      181 tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt aaatctccta
      241 ttttgtacag ttcattgtac aatgataggt gttaattaac tatttattaa ttagtttgta
      301 gatcaagaca ttgccagcga gacgaaaaat cagactttgc tctttttggt gctgtcaagt
      361 tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca aatccatcgc
      421 cataaaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca
      481 tcagttgaat aaacattcac agagattttt atgacacgcg ttcaatttaa acaccaccat
      541 catcctgact agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc
      601 atgaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt
      661 gttaacggtt cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg
      721 catagctatg cagaaatccg gccgtttaag tgatgactca cgcgaattac tggcgcgctg
      781 tggcattaaa atcaatcttc acacccagcg cctgatcgcg atggcagaaa acatgccgat
      841 tgatattctg cgcgtgcgtg acgatgacat tccctgtctg gtaatggacg gcgtggtaga
      901 ccttgggatt atcggcgaaa acgtgctgga agaagagctg cttaaccgcc gcgcccaggg
      961 tgaagatccg cgctacttta ccctgcgtcg tctggatttc ggcggctgcc gcctttcgct
     1021 ggcaacgccg gttgatgaag cctgggacgg tccgctctcc ttaaacggta aacgtatcgc
     1081 cacctcttat cctcacctgc tcaagcgtta tctcgaccag aaaggtatct cttttaaatc
     1141 ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc gggctggcgg atgcgatttg
     1201 cgatctggtt tccaccggtg ccacgctgga agctaacggc ctgcgcgaag tcgaagttat
     1261 ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa atggaagaat ctaaacagca
     1321 actgatcgac aagctactga cccgtattca gggtgtgatc caggcgcgcg aatcaaaata
     1381 catcatgatg cacgcaccga ccgaacgcct ggatgaagtc atcgccctgc tgccaggtgc
     1441 cgaacgccca accattctgc cgctggcggg tgatcaacag cgcgtagcga tgcacatggt
     1501 gagtagcgaa accctgttct gggaaacgat ggaaaaactg aaagcactgg gtgccagttc
     1561 aatcctggtc ctgccgattg agaagatgat ggagtgatcg ccatgagctt taacacaatc
     1621 attgactgga atagctgtac tgcggagcaa caacgccagc tgttaatgcg cccggcgatc
     1681 tccgcttctg aaagcattac ccgcaccgtt aacgatattc tcgataacgt gaaaacgcgt
     1741 ggcgatgagg ccctgcgtga atacagcgcg aagtttgata aaaccacggt taccgcgctg
     1801 aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc tgagcgacga gctaaaacag
     1861 gcgatggcgg tggcagtaaa gagtattgaa accttccaca ctgcgcaaaa actgccgccg
     1921 gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg tcacgcgccc ggtggcttca
     1981 gtgggtttgt atattccggg cggctccgcc ccgctcttct caacggtatt aatgctggca
     2041 actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt gctcaccgcc gccgattgcc
     2101 gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg acgtgtttaa cgtcggcggc
     2161 gcacaggcca ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc
     2221 ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc aggtaagcca gcgtctggac
     2281 ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc tgacagcggc
     2341 gctacgccgg attttgtggc ttctgatttg ctttctcagg ctgaacacgg ccctgactca
     2401 caggtaattt tactgacccc cgatgctgat atggcgcgtc gcgttgccga ggccgtcgaa
     2461 cgccaactgg cagaactgcc gcgtgccgaa accacccgcc aggcactgaa cgccagccgc
     2521 ctgatcgtga ctaaagattt agcgcagtgc gtggagatct ccaaccagta cggcccggag
     2581 cacctgatca ttcagacccg caacgcccgc gaactggtcg atggcatcac cagcgccggt
     2641 tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg actacgcctc cggcaccaac
     2701 cacgtgctgc cgacttacgg ttacaccgcc acctgttcca gcctcgggct ggcagatttc
     2761 cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt tctccgcgct ggcttcgacc
     2821 attgaaacac tggccgccgc cgagcgcctg accgcccaca aaaatgccgt tactttgcgt
     2881 gttaacgtcc ttaaggagca agcatgagca ccgtgactat tactgattta gcgcgtgaaa
     2941 acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct
     3001 ggctgaacgc caacgaatat cccacagccg tggagtttaa gcttactcag caaacgctca
     3061 accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg
     3121 taaaaccgga acaggtgctg gtcagccgtg gcgcggacga aggtattgaa ctgctgatcc
     3181 gcgctttttg cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt
     3241 acagcgtcag cgctgaaacc attggtgtcg aatgccgcac agtgccgacg ctggaaaact
     3301 ggcaactgga cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt
     3361 gcagccccaa caaccccacc gggcaactga tcaatccgca ggattttcgc accctgctgg
     3421 agctaacccg cggtaaggcg attgtggttg ccgatgaagc ttatatcgag ttttgcccgc
     3481 aggcatcgtt agctggctgg ctggcggaat atccgcacct ggcgattttg cgtacccttt
     3541 cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac gctggcaaac gaagaagtca
     3601 tcaacctgct gatgaaagtg atccccccct acccgctctc gacgccggtt gccgacattg
     3661 ccgcacaggc gttaagcccg cagggaatcg tcgccatgag cgaacgggtg gcgcaaatta
     3721 ttacagaacg cgaatacctg attgccgcac tgaaagagat cccctgcgtg gagcaggttt
     3781 tcgactctga aaccaactac attctggcgc gctttaaagc ctccagtgca gtgtttaaat
     3841 ctttgtggga tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct
     3901 gcctgcgaat taccgtcgga acccgtgaag aaagccagcg cgtcattgac gccttacgtg
     3961 cggagcaagt ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag
     4021 cgaaccgccg agtgattttc aggtggaccg ttttgacaaa ctcgcctttg aaccgggcgt
     4081 gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag ctggtgatga tcactaatca
     4141 ggatggtctg ggaacacaaa gtttcccgca agcggatttt gatggcccgc acaacctgat
     4201 gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa gtgctgattt gtccgcacct
     4261 gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa ctggtggagg gttatctggc
     4321 tgagcaagcg atggatcgtg ccaacagtta tgtaattggc gatcgcgcga ccgacattca
     4381 actggcggaa aacatgggca ttaatggttt acgctacgac cgcgaaaccc tgaactggcc
     4441 aatgattggc gagcaactca ctaaacgaga ccgttacgcc catgtagtgc gcaacaccaa
     4501 agagacgcag attgacgtcc aggtgtggct ggatcgcgaa ggtggcagca agattaacac
     4561 cggcgttggc ttctttgatc acatgctgga tcagatcgcc acccacggcg gtttccgcat
     4621 ggaaatcaat gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg
     4681 cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac aaacgcggta tttgccgctt
     4741 tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc gcactggata tctctggtcg
     4801 cccgcacctg gaatataaag ccgagtttac ctaccagcgt gtgggcgatc tcagcactga
     4861 aatgatcgaa cacttcttcc gttcgctctc ttacaccatg ggcgtgacac tacacctgaa
     4921 aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg ttcaaagcct ttggtcgtac
     4981 cctgcgccag gccatccgcg tcgaaggcga caccctgccc tcgtcgaaag gagtgctgta
     5041 atgaacgtgg tgatccttga tactggctgc gccaacctga actctgtgaa gtctgccatt
     5101 gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg acattgtgtt gctggccgat
     5161 aaactgtttt tacccggcgt cggcaccgca caagcagcga tggatcaggt gcgtgagcgc
     5221 gagctgtttg atctcatcaa agcctgtacc caaccggtgc tgggcatctg cttagggatg
     5281 caactcctgg gacgacgcag cgaagagagc aatggcgtcg acttgctggg catcatcgac
     5341 gaagacgtgc cgaaaatgac cgactttggt ctgccactgc cacatatggg ctggaaccgc
     5401 gtttatccgc aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac
     5461 tttgttcaca gctacgcaat gccagtcaat ccgtggacca tcgcccagtg taattacggc
     5521 gaaccgttca ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt ccacccggag
     5581 cgttctggtg ccgctggcgc taagttgctg aaaaacttcc tggagatgtg atgattattc
     5641 cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca tcagggcgat tacggcaaac
     5701 aacgcgatta cggtaacgac ccgctgccgc gcttacagga ttacgctgcg caaggtgccg
     5761 aagtgttgca cctggtggat ctgaccgggg caaaagatcc ggctaagcgt caaatcccgc
     5821 tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca ggttggcggc ggtgttcgta
     5881 gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc gcgcgtggtg gtcggttcca
     5941 ccgcagtgaa atcaccggac gtagtgaaag gctggtttga acgtttcggt gccgatgcgc
     6001 tggtgctggc actggatgtc cgtattgacg agcaaggcaa caagcaagtg gcagtcagcg
     6061 gctggcaaga gaactcgggc gtttcactgg aacaactggt ggaaacctat ctgcccgtcg
     6121 gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg cacgctggcg ggatctaacg
     6181 tctctttata tgaagaagtg tgtgccagat atccgcaagt ggcgtttcag tcctccggcg
     6241 gcattggcga cattaacgat gttgctgccc tacgtggcac tggcgtgcgc ggcgtaatag
     6301 ttggtcgtgc attactggaa ggtaaattca ccgtgaagga ggccatcgca tgctggcaaa
     6361 acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg
     6421 caaccatgaa atcattggcg atatcgtgcc actggcaaaa cgctacgccg aagaaggtgc
     6481 agatgaactg gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg tagataaaag
     6541 ctgggtatct cgcgtggcgg aagtgatcga cattccgttt tgtgtggcgg gtgggattaa
     6601 gtctctggaa gatgccgcga aaattctttc ctttggcgcg gataaaattt ccatcaactc
     6661 ccctgcgctg gcagacccaa cattaattac tcgcctagcc gatcgctttg gcgtgcagtg
     6721 tattgtggtt ggtattgata cctggtacga cgccgaaacc ggtaaatatc atgtaaatca
     6781 atataccggc gatgaaagcc gcacccgcgt cactcaatgg gaaacgctcg actgggtaga
     6841 ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt
     6901 gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt gaagtttgcc acgtcccgct
     6961 gattgcctcc ggtggcgcgg gcaccatgga acacttcctg gaagccttcc gcgatgccga
     7021 cgttgacggt gcgctggcag cttccgtatt ccacaaacaa ataatcaata ttggcgaatt
     7081 aaaagcgtac ctggcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgt
     7141 cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg tgattgtgca acacgcggta
     7201 tctggcgaag tgttaatgct gggctacatg aacccggaag ccttagacaa aaccatcgaa
     7261 agcggtaaag tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacc
     7321 tcgggcaatt ttctcaatgt ggtaagtatt gccccggact gcgacaacga cacattactg
     7381 gtgctggcga atcccatcgg gccaacctgc cacaaaggca ccagtagctg cttcggcaac
     7441 accgctcacc agtggctgtt cctgtatcaa ctggaacaac tgctcgccga acgtaaatat
     7501 gccgacccgg aaacctccta caccgccaaa ctgtatgcca gcggcactaa acgcattgcg
     7561 cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag caacggtaca tgatcgcttt
     7621 gagctgacca acgaggcatc tgacttgatg tatcacctgc tggtgctgtt gcaggatcag
     7681 gatctggatt taacgacagt aattgagaac ctgcataaac ggcatcagtg agttacgggc
     7741 tgagcaggtg tgatgttgtt gtcacacccg gagccattgc ccgatgcgca aacttatcgt
     7801 gcctacacat tatctcaaca cacgtaggcc ggataagatg cgccagcatc gcatccggca
     7861 aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct taaatgcaca ataatattac
     7921 ttcgcgttgt aattacgcag agcgttacgc cccaacacaa tccccgcgcc aaccatgccg
     7981 cccagaagca ctgccagaat caaggtgatt gcctttttcg gactatcgcg acgaataggt
     8041 aacgtcggtt tcatcacata gcggtaagca tgaatatcaa gatcatcaaa ctttaatttt
     8101 tcaatatcca acaggttttg acgtgtctga tagtagtttg gtgagaacac caacggacga
     8161 gtcgcttcat gcttaatcat cgactccagc gcttcgctcc ctagaaggaa caacgtatct
     8221 tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac
     8281 tgcaacgctt cctgaatctg acggatacgc agatctttct gctcctgcgc gaccacttcc
     8341 tgggttctta aagagtcctg caagtttttc cgtcccagag caatgttgtc cttgagatcc
     8401 ttttctagct cttgattcac tttatcatca acttgctgaa tgtattgggc caacttcatt
     8461 tgtgcgccct ctgcagtttg cccaacataa gaaacagtca atggtaattg ctggttctta
     8521 acagaaggtt cgatggtaag tttttctggc tcttcctgat tatccagcgt ttctgctaat
     8581 gcagagaagg cagaactgaa gcgaccaatt aacgtctcct gcaaatccga tactttcggt
     8641 gcagcctgac catagataac attcatggca ttgttatagc cagcaatttg ccccacgtcg
     8701 ggctgagtga taattgctgt tgacgtccat ttctccttcg ctactgccaa ataaccaata
     8761 gccagggcaa tagccacaat gacggaaatg ataattgtca tcttgccacg ccacaactgc
     8821 actagtaaat caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta
     8881 ttttctactc tcatcattac cctaactgac ggaaatttcc aagtccgcat agtttagcga
     8941 taatccccgt gaatgctata ggataagtga taaaaataat gaattattgc ggaggtaaca
     9001 aaagacaatc agggcgtaaa tagccctgat aacaagatgt tagtcgctgc caaagagatc
     9061 gcgggtgtag accttatctg ccacatcctt aagctcttct gccatacggt tggagataat
     9121 gacgtcggct tgttgtttga aggtggcgag atcacgttcc aggcgagagt tgaagaatga
     9181 gtcttctttc atcaccggct cgtagatgat cacttcaacg cctttcgcct tgatacgctt
     9241 cataatcccc tgaatagaag acgcacggaa gttatctgac ccgctcttca taatcagacg
     9301 ataaataccc accacttgcg gtttgcatcg acaattgccg agatcaggtt attcggcaca
     9361 gactggtagt tcgccagtaa ctgcttggta tctttcggca gacaataacc accataacca
     9421 aacgacggat tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc
     9481 tggcgagtat tcagacctaa actttctgca taactatcca gttcattaaa gtatgctacg
     9541 cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt ctgcttcagt ggagtcggta
     9601 aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc aaaacgttct
     9661 gcgcgttctg aacgctcacc gatgacaata cgtgacggat ggagattatc gtaaagggct
     9721 ttaccttcgc ggaggaattc cggagagaaa ataatatttt cagtacgata tttcttatgc
     9781 atcgctgcgg taaaaccaac gggaaccgtt gatttgatga ccataaccgc ataaggattt
     9841 atctcaacta cgtctttaat tactgattct acactggatg tattgaaata attagtttta
     9901 ggatcatagt cggttggagt ggcgatgatg acataatcag catcccggta ggcttcattt
     9961 ttatctaatg tggcattaaa gtgtatttta tctgattgca aaaactgctg aatttcctta
    10021 tcaacaatag gagatatccg atcattcagc atagcaacgc gtgacggtaa aatatctaat
    10081 gccacaacct catgattttg tgcgattaga agcccgtttg acaagcctac atagccagta
    10141 ccggaaatgg tgattttcat ttcatgctct cagaattaac ttaactgtga atcatgatgt
    10201 ttttagcatc gtgataagat gctaaaagtt ttaacgctac gttgtcagat ggtcaacgca
    10261 acgaaaaaca attcagattt tctttataag aatagctgat gtttattaag ttaatattaa
    10321 gcagtgaaat ttagtctata agatatttgg caaaaaaaag cccggtgcaa tatacgccgg
    10381 gcctcaattt tattgttggt taaatcagat taatccagcc attcagtatg gaacacacct
    10441 tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt cacgctgtgc ctggatcagg
    10501 ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc
    10561 ggaaccggga taccgttctg tactgcataa gcgacgacat cgcgcagcgc ctgctggtag
    10621 tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt tagcgatctg cggattttcg
    10681 gcataagcat cggtgatttt ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa
    10741 atcttcgcga tttcgccgta gttcagatcc caattgtatt cttcagacgc agcacgcagc
    10801 tgagagaagc cctgagcgta agaaacgatt ttgcccagat acagcgcact gcgaactttt
    10861 tcgatgaact cagccttgtc gcctgctgac tgagcttgcg gaccagagag aactttagat
    10921 gcggcaacgc gctgatcttt cagagaagag atataacgtg caaacacaga ctcggtaatc
    10981 agcgacagcg gttcgccgag atccagcgcg ctctggctgg tccatttacc ggtgcctttg
    11041 tttgctgctt catccagaat cacatcaacc aggtagttac cgtcttcatc ttttttggtg
    11101 aagatatctt tagtgatgtc gatcaggtag ctgctaagtt caccgttatt ccactcggtg
    11161 aaagtctggg ccagttcttc attagataga ttcaggccac ctttgagcag agaatacgct
    11221 tcagcaatga gctgcatatc tccgtattca ataccattgt gaaccatctt cacataatgg
    11281 cctgcgccgt cggcaccaat ataagtaacg catggctcgc cgtcttctgc cacggcagcg
    11341 attttagtca ggataggcgc aaccagctca taagcctctt tctgcccgcc aggcataata
    11401 gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac cggtaccgat gaagttaaag
    11461 ccctctgctg aaagctcacg attacgacga atggtgtcct ggaagaaggt attaccacca
    11521 tcaatgatga tgtcaccttt atcgaggtat ggtttcaggg aatcaatcgc agcatccgtg
    11581 cctgcacctg ctttcaccat taacaggatg cgacgaggcg tttccagaga ttcaacaaac
    11641 tctttcaccg tatagtaagg agccagtttc ttgcctggat tttcagcaat cacttcttcc
    11701 gtcttttcgc gggagcggtt gaaaatagag acggtataac cacgactttc gatgttgagc
    11761 gcaaggttgc gccccatcac tgccataccg actacgccga tctgttgctt tgacattgtt
    11821 tactcctgtc ttctatatta ttaaccgtct attaaccgtt atattattaa ccgtcgtgat
    11881 tttcatcttc taatcaagga atacatatgg ttacagcatg cttcttactt tatagcgata
    11941 agtatgccat tgggtgtatc cataagaact ttgaattttg gatacttttc tggattaaaa
    12001 ttagcatagc ttcgtttaga tataacaata aacgaactat taaaagattt catcttctct
    12061 attaaatcat cctgtgggat tacctcacac tcgggattac taatagtcca atcactatat
    12121 cttgctaatc caaagacgtc accaattgcc atccctttat agaagtaaat accatcctcc
    12181 aagcctaatt ggataagatt atctccatat aactcactat attcacttgc tttcttcatc
    12241 agttcatagc tatagcgact ttctaagcgt gaattaaaat tcaagtattc agaataaaaa
    12301 tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaat aatacacaca aggataaaat
    12361 atcgtcccaa cttgtaattt gtaatgttga tatcttttat taaacttgca attgagatcg
    12421 caacctgcaa tactgacaat gggtaaatgg gtgataaata cctgtcacct tgcgtaacag
    12481 aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa tacccaaagt attttattat
    12541 tcctaagtgt aaagacagac agtatagcat aaaaaacaac atcactgcca actttattaa
    12601 ttgcttgttg caaatctaat gtggcaggac ttataccatg tcgagcttgt tctgctgttt
    12661 gaacttgtag atcaatttca ttccataggt agtgaccaaa tatatttcct ccagcaggac
    12721 taaaaggatc accagagatc caaaaactac ggacatacca ataaatgcca ccaagaattg
    12781 ttgccaagct aaaaataaga aatgtctgta tgtttctctt tttcgctatc aatataacaa
    12841 atatgatggc tccatatatg gcaccaaata gttttacgcc acaagcaatt ccaataaaaa
    12901 aaccacataa aaaaattaat tcattttgtg ttttattgtt ttctgagcga atatatataa
    12961 gaatactggc tgcagcaaaa caaagatagc gttaaatcaa tatatgcata accaagatga
    13021 tctttaagtg gattgatagt aaatagtagg attgtagcaa taatgcctgg ataataagtt
    13081 ttcgtaattt cccatgaaca gccaacaagt ccacaggcta ttacaaaaaa acagatattt
    13141 gctaaaaact gagctaacct tacatctcct aactgtaaac ctaaagccat taaaagatct
    13201 gcattttgcg ggaacagagg aaaacgcaaa tattgttcaa caacaattgc atggtgctca
    13261 actaatgaac gagccaaagg taagtgatac atagtatcat cccaatcacc gggagctcgc
    13321 agggacgata acaatgtaac cacaaaaatc aaaacaagaa ataaaataac aaacagcgta
    13381 ctgaactcaa tattatttat tgccagccat ctaaattgct gacgtttaat aatgcttaaa
    13441 acccaacaca ccgtaaaaat taaaaagata gcaatcttat taaattgccc gaccatgccc
    13501 agaaagtaaa ttatcaaaat atttaaagac atccctatgc ataggcaata aaaaataaag
    13561 aaaatttgtt aagtcctgcc accccctcct taaaaaaaga tgacaataca atattaccaa
    13621 tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac atacaaatac aacggcaatt
    13681 caatcatagt aaaatactct atttacatta tcagtttata gtttttttca gaaagataaa
    13741 ataatgcctt catttcacgt ctagttgttg tatttgaatg caaaataatg ccacatacaa
    13801 atgccattat tgacgataac atgatggagg cagttaaaaa ggcagtcgga aatcttctca
    13861 ccaaacctgt atcaatataa tcaacaatga tgggcaatcc taaaattata gacaaagcag
    13921 caagtaaaac gccgataata ctaaaaaagt atagtggccg ttcaagcgag taaagtttaa
    13981 ttattgtttt cagaatcttg aacccatcac tccaagttga tagtttgctc acactccctt
    14041 cagatcgctc gccatatttt gtattaacct caccatactt catacgtaat tctaaagcat
    14101 gaattgtaag ctcagtttcg atttcaaatc cccttgatag acatggaaaa gatttaacat
    14161 agcgtctgga aaatacacga taccccgaca acatatcagt aaaattccct ttaaagatag
    14221 tctgaacagt accagtcaac agtttatttc ctcagcgatg cccttttctg tatgtatttt
    14281 gatctccatt ctcttgacga catcctacaa ccatatccaa ttgatttttt atgagatggt
    14341 taatcatttc tggagcagaa cttgcatcat aagtgtcatc tccatcaacc attagataaa
    14401 tatccgcatc tacatctgag aacatacgtc ttacgacatt ccccttgcct ttcagaggaa
    14461 cagagtgaac tgttgctcca gcatcctctg ccacttgtgc tgtactgtca gtcgaattat
    14521 tatcaaatac atatatagaa gcagttggaa tattcgtttt gaaatcattt atgacttttg
    14581 ctattgcctt ctgctcatta taacaaggaa taattacagc gatattaaaa gagtgataca
    14641 taagcccctc atataaacta tttagctaat gtgcaagtaa ttgtgttttt aacaaatcgt
    14701 ttactaacga aaaatgaaat attttgatgc gcaatatcct aaaaccaaac taaaaagaga
    14761 aaaggtgaac aatgtaaata ttggagataa agctaagaga tcaaataaaa cgccgaataa
    14821 ataactcatc gcccccataa aaaatataaa tataaaatat ctagccttgg ttgcagatac
    14881 tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt gctgcacata taaaaccaac
    14941 aatgttactt cggccttgac caaaagctaa attattataa cacgcataaa atacccccca
    15001 atgtaaagca gtattgacaa ttccaattaa aaaataacta aaaaaagaac tggtcaataa
    15061 cttccctatt tttaacatcc tttattttgc tccagaagtg aggttattac taatttggat
    15121 attttctata gaaaataccc ataaaactac aacgcctata ataaaaattc ccatgggtgt
    15181 aaaaagaaat ggattagtta tagaaacggt aatactgaaa aaaagtatta acgacatcat
    15241 taatgatgtt tttgtactat ttaaaaaata gcctttcaaa aagataagca acataggaac
    15301 taatgatata aatacaccaa taacgcctgt tttctgaagt atctcaagag gtactatttc
    15361 aatccttaat cgatctaata taaatgaacc aaatcctctt ccaaacaaga acgtcgctaa
    15421 atcaacattt ttataataaa aatataaatc attaaatcta acagagtcag aatcttcact
    15481 gcgtgatccg accatgtaaa gcataattat aaatagaacg ctgcccaata ttattataat
    15541 agtatttaaa tatagcttat gtaatttaaa actggctaat aacagtgata atgttgtaaa
    15601 aagtaataca cctcttgatt ctgtcaataa tatcgcaagc actattatga gacattttaa
    15661 atattttttg tcgacaaaac tgataatcgc accgacacca aaaaagtaga aggatttgga
    15721 aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc aatgataagt gttcataaat
    15781 taaattaaaa tttaacaacc cgaaattcaa caaaattatg tatgaaatat ataacagtga
    15841 tccatataat gcaaatgcat taactatttt aataaaaatc tcacatgaaa atacttttaa
    15901 agtatatctt agtgtaaata gatatataag tgacagcata aaaaggtatg gttgcacatc
    15961 aaacagtata tcatttattt tattaccagc taataagccg acaaagaagc cataaaaagg
    16021 aaataaaagc aaccatataa aaagactgac acatttttgg gtgattgaaa aattgaatgt
    16081 aactaaatta aaaattaaaa gtataaaact aaatacaaat aataaatatc gcaaagggaa
    16141 tattcccaat ggctccagta atcttccacc accaccaata atcagttcaa tacataaaaa
    16201 tgttataaaa attttattta tattattcat attcgtaagg tgatgttttt taaaatatgt
    16261 ttaaaatcaa cattaataaa atataaattt tatcagcctt tttccgcctt aacttccaaa
    16321 aacttaaagc caagatcaaa ttgcctatat aacctttacc caattgatta ttcacaaagc
    16381 tatcatcagc taaggcatta gctattttag ttacttcttt tcgataccac ttgtttctca
    16441 ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt tgctcccact tggttgtttt
    16501 catgctgtct gtacagcatt gttggttttc tatcaatata ccacgaataa tttctcgtcc
    16561 gcgcaaatga atataaaaac caatcatgca gacagatatc ttgtgcagca tttttattat
    16621 ttatgatgaa cttcttaaat tcaataagcg tttctttttt tataacatat gtacaccccg
    16681 gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga ctgagcctta tcaactaaat
    16741 tttttcgccc tgatggataa tatgcaatta cattgctgga ataacaattc gcattattaa
    16801 ataccaagca gtcaattgca ttttttattt tatctttata ccaaatatcg tcctgatcac
    16861 taaaacagat ataatcatag ttttcaagat cgacatcttt aattaatcta taaaaatttt
    16921 tacctgcccc cccaaactta ttgacgcttg gaagtatatt tataattgag ggatatgata
    16981 attgaaattc ttcacaaata tttaatgtat tatcagttga taaatcatct gatataaaaa
    17041 ttgagatgtc tacatctttt tgttcaataa tactttttaa ttgttcctca atccagcatt
    17101 cgccattata cgtagccata agtacagcta ctcttttttg atcagaacta tgcattataa
    17161 cgaccgcccc cagtaattcc tcttattcca tccagcgtag ccaaaatatg ttttttcata
    17221 tattcaattt tatttttttg agttgcgata attaaagtcg tatgaattaa agaaaaaaca
    17281 acttccctaa tagagagtaa aagaggcaca tgattttttc taagcaaaag aaatgcattt
    17341 cttactcgat aataacgacg tactggcgag tgaattggta tgttaatccc acagattttt
    17401 actgacttat ccccaatagc atgaaccatt tctatatcag gaatgatatg aactaatatg
    17461 ccatagttta agcatctaag acaccattct gtatcgacat aatcaataaa aagttcctca
    17521 tccatcattc caacaatctc aaaaacttct tttgaaacca tggttccgga tgagattgtt
    17581 actgaacttt taaacggtgt ctgtccttca gtgacatgaa ttttctctcg aagaccattt
    17641 tttttgatat tacaaatagg ataataatgt gacttatctc tatcaataaa aactgggcca
    17701 gctatttttg cattttcatt atccatatag caggaaagtt ttttgataaa ctcattgcga
    17761 atacggctat cttgatcaaa aaaaattatc ttctctgccc ctttttcaag agaatatcga
    17821 atcccaatat tttgtgcttt tgctatacct tcattcctac caagacaaat taaatgaact
    17881 ttttcaatgt ctgctaattt atctttcaag gtgaaattag tcctattatc gactactaca
    17941 acgtaacaat tttgctcctt tagttcagtg atcagtgcat tcagattttt aagttcggga
    18001 ttatatgtca caatgacagc gtaaatatta ctattcatta tccaagtgac tcagtaattg
    18061 gttaatttta gttttcatat acagaacaca ttctagtata aagccattgt aataacagag
    18121 catctaaaat tacacgtatg ctccaggata ttgcggcacc cagtaaccca tatttttcga
    18181 ccataaaata caaaagggct aaataaggaa ccaattcagc acaatgaatc aaagcagtaa
    18241 ttttagattt tcctaatgcc tggattgcag agaatggtat cattgccagc gcattgaaga
    18301 aaaaaccgat caataacaca tttaatatat taaaggaatt aattttataa ttctcaccaa
    18361 accatatttt caatacaagc cctgaatata acaagcctat gattatcacc ggcaaacaga
    18421 ttagaaacat taaaagcaat gatttattta cattacgttt aaattcttta aagtctttga
    18481 tgttacttaa ccttggaaac actgccctcc cgattgctgc aggtattatt cctaatttta
    18541 gaataacctc cgctggtgca gaataaaatg caactttatc tgcccccata atatttgaca
    18601 ctataaatcg atcaaaatac accatgaccg gacttatgat attacttact gtcatccaac
    18661 cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa atgaaccccc gaaattttaa
    18721 tatcattcct gacataatag gcagagacta aaatcgaaat aactcttgca aatattaaac
    18781 cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa tatggccgga attcccgcga
    18841 tgcaagagct tgatatagat ttttgaatat ttacaatgcc aaatttttca tccccctcaa
    18901 gaatggctga ccataattga tttagaataa ataatggaat gcaaatagcc aacagtttaa
    18961 atgctagttg tatatcacta tgttcaacac cagaaatttt tagataatta acaattccat
    19021 cagagaaaat caataataaa aaagcaccaa agcatgaaaa tagcaccaaa aaagatgtac
    19081 ttgttgaaat taccttggtt ctttcatgat gattatcacg atgaattgca atttctctaa
    19141 taacagagcg agtcaggcct acatcaaaaa ttccagcata cccaacaagt gcaattgcaa
    19201 ttgtataaat accgaaacct tcaggcccca aacctcgagc gagaaatcct agcgagggga
    19261 tagcaattaa agttggtatt gcatagccaa aaaggttcca gacactattt tttattatgc
    19321 tcattattac atttctttat catataaaat aatcagcatc tacaaatttt tgaccattaa
    19381 tatctttatc agaaagcaat ggattttgaa ccggccattc tatatttata cttttgtcgc
    19441 tccataaaat cccacgctct tgcatttttg aatagaaatt atttgttttg tagacaaatt
    19501 ctgcttcatc actaagaacg caaaacccat gagcgaatcc ttccggtatc cataattgac
    19561 gcttattctc cgccgacaaa tttacaccaa cccacttgcc aaatgttggt gatgatttcc
    19621 gaatatcaac agctacgtca aaaacttcac cgacaacaca gcgtaccagt tttccttgag
    19681 cataaggttc cagctgatag tgcagtccgc gtaacacacc cttagttgat ttagaatggt
    19741 tatcctgaac aaactcaacc tttcgtccta cagcctcttc gaaaactttc tgattgaagc
    19801 tttccataaa aaagccccgc tcatcaccaa atacttttgg ttcaaaaatt agcacatcag
    19861 gaatttcagt tttaattacg ttcattttat taataacctt taatcatttt cagcagatac
    19921 tgaccataag catttttctt cagcggctcg gctaatacct tcacttgctc agcatcaata
    19981 aagcctttac ggtaagcaat ttcttccggg caggaaactt tcagcccctg acgctcttca
    20041 attgttgcaa tgaagttgct tgcttcaata agactttgat gtgtccccgt gtccagccat
    20101 gcataaccac gccccatcat agcgacagac aaacgtcctt ggtccatata aatacggtta
    20161 atatcggtaa tttccagttc gccacgtgca gatggtttaa ggtttttcgc catttctaca
    20221 acgtcattgt catagaaata aagcccagta accgcatagt tactttttgg ttccagcggt
    20281 ttttcttcca gactaattgc cgtaccgtta tcatcaaatt ccaccacacc gtagcgctca
    20341 ggatcattta cgtgataggc aaaaaccgtt gcgccactct ctctgttaac agcggtatcc
    20401 attaatttcg gcaggtcgtg accatagaag atattatcac caagtaccag cgcacaatca
    20461 tcaccaccta taaattcttc accaatgata aatgcttgcg ccagaccatc cggactcggt
    20521 tgtactttat attgcagatt aagcccccac tggcttccat caccaagcaa ttgttgaaaa
    20581 cgtggtgtat cctgcggcgt actgataatc agaatatctc gaatacccgc caacataagc
    20641 gttgaaagcg gataataaat catcggttta tcataaatcg gtagcagttg tttgctcaca
    20701 gccattgtca caggataaag acgagtgccg gaaccaccag ccagaataat acctttacgc
    20761 gttttcattt caccattcct tttaattcat ctcgctctag catcatgagc gagatgcaat
    20821 aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc atacgtttca cgcccacctg
    20881 ccagtcaggc aagacaagcg caaagttctg ctgaaatttt tcggtattga ggcgagaatt
    20941 atggggacga cgtgctggtg tgggataggc cgttgtcggc acggcattaa gtttgttaag
    21001 agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc agagcggcat aatcgtgcca
    21061 ggttgttgta ccaccagcaa ccagatggta caaaccagca acttctggtt tatttgcggc
    21121 cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca gcccccgttg gtgcgccgaa
    21181 ttgatcgttt attacagcca actcttcgcg ttcttttgcc aggcgcaaca tcgttttggc
    21241 aaagttattt cctttaccag cgtataccca gctggtacgg aaaataagat gctttgcgca
    21301 atgttcttgt aacgcttttt ccccagctag cttggtttca ccgtagacat ttagcggtgc
    21361 tgttgcatcc gtttccagcc atggcgtgtc accgttgcct gggaaaacat aatcagtgga
    21421 gtagtgtata acccaggctc cgacttcatt agctgctttt gcaatcgctt cgacgcatgt
    21481 cgcgttaagt aattgcgcga aattcggttc tgattccgct ttgtctactg cagtgtgagc
    21541 agcagcatta acaataacat cggggcgaat ttttttgacg gtttcagcca caccttcggg
    21601 gttgctgaaa tctccacaat aatcagtgga gtgaacatcg agcgcaatca agttacccag
    21661 aggagccaga gcacgctgca gttcccaacc cacctgccct gttttgccga aaagcaggat
    21721 attcattact gacggccctc atagttttgt tcaatccacg actgataagc accgcttttc
    21781 acattctcaa cccaatttgt attagccaga taccattcca ccgttttacg aatcccgctc
    21841 tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa ttttatcggc atcaatggcg
    21901 taacggcggt catgccccgg gcgatcggca acataagtaa tttgctcacg ataagatttc
    21961 tctttcggta caatctcatc caacaaatca caaatagtga gcactacgtc gatgtttttc
    22021 ttttcgttat gtccaccaat gttataagtt tcacccgctt taccttcggt tacgacggta
    22081 tataacgcac gagcatgatc ctctacatac aaccagtcgc gaatttgatt ccctttacca
    22141 taaataggta atgccttacc ttccagagca ttcagaataa ccaatggaat aagtttttcc
    22201 gggaagtgat aaggaccata attgttcgag caattagtca caatggttgg taaaccataa
    22261 gtccgcttcc aggcacgtac caaatgatcg ctggaagctt tagacgcaga atacgggcta
    22321 cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg cttcgttatt atttgcttca
    22381 tccggatggg gtaagtcacc ataaacttca tcagtagaaa tatgatgaaa gcggaaactt
    22441 tttttctttt catcattcag agcagaccag taattgcgcg ccgcttctaa aagaacataa
    22501 gtccccacaa tattggtttc aataaatgcc gcagggcccg ttattgaacg gtcaacgtgg
    22561 ctctctgccg ccaggtgcat caccgcgtct ggctggtgct gtgcgaaaat acgcgacatg
    22621 gctacagcat cgcagatatc tgcatgctca aaagcataac gttcagaatc agaaacatca
    22681 gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga cattaacaac actatcttgc
    22741 gtattattta ttatgtgacg aacaacagca gaaccaataa accctgcgcc accagtaaca
    22801 agtatcttca cttttttatt ccatatagct atagagcatg ctgtgaaata gactgctcca
    22861 gatttgatta atagaggtat taatacacgc taccgcccct ggcttaacag ctaccagtgc
    22921 actgcatgca tctcaacgat gtggcgagcg ttagccactc gcgccaaatc cgaaaaattc
    22981 aaacgttaat tgtcttacca aacagccgta gaaacaagga aaatcctgga aaaatttgaa
    23041 taatatgagc tatgtaactt cttgttttta taggctttat aaaaacggca acggcaggaa
    23101 aattcaatga gcaaaactta tgccacgaat cttcactgcc gttataattt tcttatcaac
    23161 cgttacatcc ggtcagattt tcattattcg cttaacagct tctcaatacc tttacggaac
    23221 ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa acgcctgcat atagcccatt
    23281 tttttgccgc agtcgtaact gtcgccggtc atcagcattg catcaacgga ttgttttttc
    23341 gccagctcgg caatagcatc agtcagctga atacgtcccc atgcaccagg ctgagtacgt
    23401 tccagttccg gccaaatatc ggcagaaagc acatagcgac ctacggccat gatgtctgag
    23461 tccagcgtct gcggctgatc cggtttttcg ataaattcaa caatgcggct gactttaccc
    23521 tcacggtcca gcggctcttt agtctggatg acggagtatt cagagaggtc acccggcata
    23581 cgttttgcca gcacctggct gcggcccgtt tcgttgaaac gtgcaatcat ggcagcaagg
    23641 ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa cgtctggcag taccacgaca
    23701 aatgggttgt caccaatggc aggtcgcgca cacaaaatgg agtggcctaa acctaaaggt
    23761 tcgccctgac gcacgttcat aatgatcacg cccggcggac agatggactg tacttccgcc
    23821 agcagttgac gcttcacgcg ctgctcaagg agtgattcta actcataaga ggtgtcgaag
    23881 tggttttcga ccgcgttctt ggacgcgtga gttaccagga ggatttcttt gatccctgca
    23941 gccacaatct cgtcaacaat gtactgaatc attggcttgt cgacgattgg tagcatctct
    24001 ttgggtatcg ccttagtggc aggcaacata tgcatcccga gacccgctac aggaataact
    24061 gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg ttgctgaatt atagcttgtt
    24121 cgattttttt cgccagcatc aattaccctg aattgattac tgaattactt gtgatgttac
    24181 gccgcttcgt tgtggattgc agtagcattg ttcctaagta tggctccatt tttccaggaa
    24241 tggtcgcaaa tctactccct ccgttccggc aatctaaagt taatcttttc cacattaaca
    24301 atatggtgat taatcctgtc gatatcgacg gagctttgcc ctctttcatt caccgcatga
    24361 acatttgcaa gagacagcag tgtttctttt ttcgccataa agacgccgcg aacgtctttg
    24421 cgcatgtcga agttcatgct caatgctggt ccaactgagg attcctgcat cacattgata
    24481 tttcgcataa aaagatgttg cggcttgttg tgtaactcca gcgaggcacg cttcatctca
    24541 atgttagtta gcgacacaaa ggagacggca ttcccggcgg agatttggat gccgcgcaat
    24601 ttataagcaa gatgggtatt atccagttga atattattca ctcggaaatt ttgcggtatc
    24661 gagagatatt tgcctttaat taccccatag ccgattaaca tcccggcgct attaatcatt
    24721 tcaatattat caatcacgaa attgtcacaa ccgtaaatag cgactgtcgc gttatcaatg
    24781 cccgctttct tactgaaatc cggcgtgata ttgcgggcat tgatattacg aataacaaaa
    24841 tgtttaccat tttcaacatg gatcaactgc cgacaatccg atcccgtgat attcgccacg
    24901 acaaagtttt tcactgcctg gtcttccggg tagttgttat cataagtgct tcccgcaagg
    24961 cctatgccga tgccccagtt gattttgccg ttggtacagt tgatacgctc gatgacatgg
    25021 tcagatatca aaatatcact gtcgttaatt gccacgttcc attcaatggc gtcgccctgt
    25081 aagtcgctga acttacagtt ggtaatgttg gcaccgataa tctggttatg aaatccctgg
    25141 cgtaagatgg cgtaattagc gtggctgaca gtgaggttat cgatggtcag gttgcgcatg
    25201 acccgtttgt ttttgccgcc aatataaatc tgcattaccg ggccaaagcc gctcatcgcc
    25261 agccctttga tggtgcagtc agaacaacgc acatccaggg taatgttatg cataccgccg
    25321 ccctctcccc ctgtcacctg gctaccgtcc tgtaagacaa atcgccctct gccgttgccg
    25381 cgcaggcttc caaggatgtg taacgttttg ccgggaggga tgaagatgcc ggtgttgata
    25441 ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc cttcgctgaa ggcttgttta
    25501 aatgaggcga tccagtcatg tgggttgtag tcgttaatgt taacgctttg tcgggcggga
    25561 agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg agcttgccgt caggaaggtg
    25621 cgtcgggaga gtgttttaaa tggcatacgt tctcctctat aaagcctgca gcaagctggc
    25681 gagttctcga ttaatcacct gctggttaaa atcgtgttcg actttttcgc gcgcacgttt
    25741 gacgaccgga gccagttcgt cggtatccag ttggctaaac gccgccagac gttgtgccag
    25801 tgcgcgagca tcgttctcag gcagcagcca gccggattta tcggcctcca ccagttcagg
    25861 aattccgcta tgcagagtag aaaccaccgg aataccgacc gccatcgctt ccattagcgc
    25921 caccggaatg ccttccatat cgccatccgc gcccgttacc gatggcaaca ggaagacatc
    25981 cgcgtcgtcg agcatctcct tcacttcatg gctcggttta aagcccggca tctctaccac
    26041 atcttccagt tgatattgtt cgatgagggt gcgcaggcgt cgttcccaca ggccaatgcc
    26101 gaggatgcga tagcgaaatg ccacgccctg ctctttcaac tgacggcagg cttcaatcgc
    26161 cacatgcagg ccttttttct cggttaagcg tgcgacagat atgatttcca gcggcgttgc
    26221 gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc acgcccatac gcgatacggc
    26281 gattttttct ctggggcagc ccattttttg cagccttccg gcccacagat cgcttatcgg
    26341 taacatcagg tcgccacggc gaaacagttg ttgatattcg ggagtgtagt ggttgagcac
    26401 ttcccgactg gagatatcaa taccgtggaa gatggtggca attttgccgc gaatgatacc
    26461 cagttcgcgt agttttgctg cggttacccc cgctgggcca aaatgagcga tgaacacatc
    26521 ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac aaaatcaggt tccgcgactc
    26581 ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt ttacgatgaa tgccacgtaa
    26641 ggtctggctg gcgcggtggc gcagtttcgc tactttgccc gcaggttcgt cctgtaacca
    26701 gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg tgggtgttct ctgtatcgcc
    26761 tttttgcaaa gcgacaatct ccacttcaaa gcccatatca ataaacgcgg taatttgatt
    26821 gaggacgaag gtttccgacg acagcgggaa tttcaataag aagaagccga ccttcatttc
    26881 acctccccga tgcgctcaag cacagactgc accatctgca ttcccgtctg acgttcgcga
    26941 ctgacggctt cgttaaatcg agcgttcagc accggaagct ggcctaaggt atccgcaacc
    27001 atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa tcgccatctc tggcagtccc
    27061 agctgctgca taatcccggc ggatttatgt tcatagttga tggcaatcgc cggagtagca
    27121 aagttcattg agataatggc ggagtgcagg cgcgtaccga cggtgagttc acaggctccg
    27181 agaattttgc ccatttccag atcgttgagt tcatccatca ctacgtggta acgggcagga
    27241 tcgctgatgt gctggcgcag attgagcgcc accatgcggt cgtctttgtt atagctgtcg
    27301 atacccgtac aggtggagag cgcaatcacc tgatagcctt catcgagaat gcgattgacc
    27361 accccggcaa aggctttttc atacgcttgt tgagtggtgc cgagacgttt gtcgaacggt
    27421 gccagttcgc gcagggtaat tgccactgtt ttctgttgtg cggcaacgtc cagccagtgt
    27481 tgaacggcat agctggcggt gaagtcttcg gtgtggtgat cgaccagcca cgcggtatcg
    27541 acgccatgtt ccacttttgc ggtggtgata tttctgcgtt tcatcaaatc aaggctgacc
    27601 gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat agttcgccag ttggttaaat
    27661 tgctcatcct ggaaggggcc gacgctgtga ccaatcataa acagcggctt tttcgccata
    27721 aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga gatcgacaaa aaacgatccg
    27781 ccgacctgaa taatggcgtc gtaacctgac agcaggcgca cgaagtcggt gaatccctgg
    27841 gcgatggcga tattgcgcag cttgccagtg tcggtgacgc gtgagagcaa tacctgatgc
    27901 tggtagcggc gacggaggac ttttttaatg cgcccgacaa cgcccgccgc gctattgtgt
    27961 tgtgtcatct gcaggaacag cggatcgccc attaccgggc ggttgagcag ccaggaagaa
    28021 ctgaccggat agcggctcat cacgtccact tcggtatgtg gattgaggat gttgatggca
    28081 tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc cgcaagtgtg gttgcccaga
    28141 ataagtaatt tcattttttc ctcataaatt tgatgccagg tgaggctgtg tttgttgtgt
    28201 tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt atcaggccta caggtcccga
    28261 gcacagaacc gtaggacgga taaggcgttt tacgccgcat ccggcaaccg ttgtcggaac
    28321 cgaaaacagc aattcacccc gcccgtaaaa gcattttcat tttttcgctg cgacaaaact
    28381 gacgcttcac ttccaccacc agcggatggc gtgacagcac aatcatcacc acaaacgcca
    28441 gcacgcccgc ggctatttgc accgccagca gcatccccag cgccagttgc cctttcagca
    28501 caatgcccag cgcataactg accaccagcg tcggcaacga gagataaaac ggcagccaca
    28561 aactcaggat gtactggcga taactggaac caagaaccgg tttaatcatc acgaagtaac
    28621 tcagaatggt gttgataatc tgcaccagca ggaaaccgag cgtgacgccg attgcgcccg
    28681 ccatctgccc accaataaca atcgccggaa taaacagaaa tgttttgaat acgttgaatt
    28741 taaagctgat atcgacccgc gctttcgcca tcagcagcga accaatcggg ttacccacgg
    28801 agcgcagcag acccacaata cacagcaatt gcagcaccgg aataatgctg ttccactttt
    28861 cgccgaatac cagcggtacg aagttattcg acaccaccat cagccccagc agcgccggaa
    28921 agttgataat ccccactatc gacagcagct tgtagaagtt aacacgcagc ttttcggtgt
    28981 cgtcctgaat tttggcaaac gccggaaaca acacgcgggt gatgattggg ttcagcttca
    29041 tcggcggtac gacggccacg ttgtacgcca ggttgtatcc ccctgccacg cccgcgccaa
    29101 gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag atagttgatg atgctgtccg
    29161 ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga cgccagcgag aagtgcagac
    29221 cggggcaata aattttgcgt ccaaagtagc caaacagcag tgttctcacc gcactattga
    29281 ccagataacc gaggatcgcg gtcatcgcca gcggccagaa atgggcgcta actaccgtaa
    29341 aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc gattttgttg aactccagct
    29401 ccttctgcat caacgcgcgg aactgttgcc cgtgggggat caccacaaac gccagcgaca
    29461 atgttttaat caacggtgcc aagtccgggt tattcagcac gtcgccgatg acatcactca
    29521 acaaaaacac cgccacacat accacaagcc ccagcccgac gttcagccag tacagcgtgg
    29581 tgagttcaag gtgactgatt tcttttcgct gaataatcga gttagcgata ccgaagtcag
    29641 aaagcgtatc tgccagcgcg ataatcacca gcgacacagt cagcaggccg aactggtggt
    29701 tgtcgataat ccgcgccagc acggtcatct gcaccagccc gaggccgatg atgatcaccg
    29761 tggcaatagc cgaccacttc gcgccgctga tggttttttc acgtaagctc atatcaatat
    29821 gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa cgattttgat atcgaaccag
    29881 acgctccatt cgcggatgta ctcaaggtcg aactcgacgc gtttttccat tttctccagc
    29941 gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc cggtaatgcc cggtttcact
    30001 ttatggcgca gcatgtagcc ttcaatgagc tggcgatact gttcgttatg cgctactgcg
    30061 tgcggacgtg gaccgacaat cgacatcccc ccggtcagca cattgataaa ctgcggcaat
    30121 tcgtccagcg aggtgcggcg cagaaagttc cccactttag tgacgcgcgg atcgttctgc
    30181 gtcgcctggg tcaccacttt gtcgttctcc atcactttca tggaacggaa cttccacact
    30241 ttgatcggct tgccatccat accatagcga gtctggcgga aaataaccgg ccctggtgaa
    30301 ctgagtttca ccgccagcgc aatacagcac agcaccgggg agatcagcag cagaataagc
    30361 gtcgccagca caatgtcttc cgcacgtttt agcaggcggt taacccccga aagcggcgtg
    30421 tcatacagcg gcaccaccgg aacgccgttc atctcttcga gacgtgaatg gagaatgttg
    30481 aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg tatccgccag ttgatggacc
    30541 agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga tatagacgtt atgaatcttg
    30601 cccgcttttg catcctcgac cagctgttgc aggttacccg cccagtcgtt agaaacgccg
    30661 cccggtttcg ggtcgtggta aacgcccacc acttcaaacc ctaaccacgg ctgattacgg
    30721 aaactctcca tcagcatttg tccggcggct aaatccccca ctacggcgac catccgcttg
    30781 ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa tacatgaacg gcaaaccacc
    30841 agtccgatgc tggtcagccc ataccacgcc agccagattt tcagttgcgt gtcgaaatca
    30901 ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta aggtccagtt ttgtagcaac
    30961 agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc gataaaaatc ggtgatgccg
    31021 cccagcatct ggaacaccac cagcgtaatc agcgccacca acaggtgcat gtagaggaat
    31081 gacagtccgc tgacttcgca aaccagccat agcccggcaa acatgatggt gatatctgaa
    31141 aagcgttgca ccatagagat taacgatgca ttggttttcg ctcgctcgcg cttttttaga
    31201 tttgtcatcg ttgttcctgt tattagcccc ttacccgcag tggggtaagg gaagatccga
    31261 cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg cttccatcag cggcacatca
    31321 ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat tggaggtgcg caggttaaag
    31381 caccagtcgg caaaggtcat gctgatgcca tcgctgcgat ccaccgccag cgcctcacgg
    31441 ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt gcgccagttt gctgttgatc
    31501 tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca gttcgcccag cgtttttcct
    31561 ttcaggcaca ccagttcggc gaccagcagc cacgggatca tgccgctgtc gcagtaagcg
    31621 aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt agatggcgtc ttccttgcgc
    31681 atccgttctt taataaaggc gtgtccggtt ttcgacatta ccggcgtgcc gcctgcagca
    31741 gtcaccacat caatagtgtt ccaagagaga cgcggatcat ggatgatctt cgcgccggga
    31801 tttttttcga ggaacgcttc tgccagcagg ccgacaatgt agtagccctc gataaactgc
    31861 cctttttcgt caaacagaaa acagcggtca aaatcgccat caaaggcaat gcccatatcc
    31921 gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac attccggcag cagcgggtta
    31981 ggaataccgt tggggaaatt gccgtccggt gtgttgtgta ctttgattaa ttccacgggc
    32041 gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca ccggacccgc tgcgccattc
    32101 ccggagttga tcaccagctt gagcggcgtg agatttttga cattgatata accgaacagg
    32161 tgatcaacgt aagcgtcacg caggttgatt tgctgatagc gaccgcgttt ggtttcatcg
    32221 acgggaggaa agtcgttggc ttcagccaga cgctggacgt cgcgcagtcc ggtatcaccg
    32281 ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt tgtaatccat cggattatgg
    32341 ctggcagtaa cttcaatgcc gccatccacg ccgagatgga acgtggcgaa atagatctct
    32401 tcggtgccgg acataccgat atccagcaca tcgacgcccg catcctgtaa accttttgcc
    32461 agcgccagtt ttaaggtttc gctggtgaga cggacatcac cgcctaacac aatggttttc
    32521 ggtttgagaa attcgccata ggcgcgacca atgcgccagg cgatatcttc attcagttct
    32581 tcgcctaatt tcccgcgaat atcataggct ttaaagcagg ttaatttttt catgtcgtta
    32641 ccctttttca ggcaatagtt ggccctgacc gaaacggcca attttatttt tgtcgttatt
    32701 tacaccgcgg tttcgcattc attgcctgat gcgacgctgc cgcgtcttat caggcctaca
    32761 aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc cgacaaacag cgcctgatgc
    32821 gacgtttaca cccgtccgta gcgatccgcg aagcgcacca catcatcctc ttcgagataa
    32881 gagccggagc gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcagtgc
    32941 gtcgccccca gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca
    33001 atggtgactt ttgccgttcc cgcgaccact acccagtgtt ccgcgcggtg atggtgcatc
    33061 tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc
    33121 gcgtcgatag agtcatattt gccccacgga cgatacactt cgcgatgcac ccgatgctca
    33181 tggcgaccat cggctttgat ctgctcgacc acttttttca catcctgtac cgcgttacgg
    33241 tcggcaatca gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg
    33301 gtgaccaggc ctgattcggc atacacatag ctgttttcag ttttgtgatt aatcacatcg
    33361 ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct cccataatga agaccaggaa
    33421 ccaacatcgc tccagcccgc atccatcggc accacaacag catctgccgt aggttccatg
    33481 accgcgtaat ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata
    33541 aaatcgagat ccggatcgac ggcgctcatc gctttttcgc aggcatcgag gatatccggg
    33601 cgatattttt tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc
    33661 cagtaatatt cgccgcttgc tacataggcc tgggcggttt ccagattcgg tttttcgaca
    33721 aactgcgcca cttcaaaggc caccgtatcc tgctcaccca cagacacttc accgcgacga
    33781 atatagccat aaccggtttc tggtagatcc ggcacaatgc caaaggtcac cagcttgccc
    33841 gcttcggcat aaggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc
    33901 acatgatccg ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc
    33961 gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc ctgccggttc gagaataatg
    34021 ttctcggtga gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca
    34081 ttgcaaatca ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc
    34141 agcatggtga gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc
    34201 ggccataaac ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc
    34261 ataattatcc ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagcgtgc
    34321 gttcggcata ttcacgtgcc accgtgttat gtttgggcag caggagcgcc tgacgaatcc
    34381 ccgccaccag cgcctcgacc gattccggtt ctacgcaaac cgcaataccc ggaaaggttt
    34441 cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg
    34501 ccagaatatt ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca
    34561 ccagatggca atcgcccatc ttcagcagtg caggtaaagc gtcatacgat tgcagcggga
    34621 aaaattgcat gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc
    34681 cgctctgccc gacaatggca aaaatcagcg gttcatcgcg caggagatcg gcagcttcaa
    34741 taacgttttc cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt
    34801 tatccggcag gccaagctgg ttacgaaggg catcaacatc ggcgtctgca acatgctgaa
    34861 aacgggcaat ttccgaccag ttggggaaga agatgacgtt atccgccgcc acgccttttt
    34921 cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac gttatcgacg ttatgcagtc
    34981 cgctacgttc gaacgccgtt gccagctgtg ccactttgcc gcctttgcct tttccggcaa
    35041 ggcccagccc cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac
    35101 cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc actacgccaa
    35161 taatgcgatc cggcttccag cgacgttgcg ccatcagtgg aaagaaactg ctgacggcaa
    35221 aactgccgag atgcaacaag cgtttcaggg tgctcggctg ttttggcacg tacagcgggc
    35281 agcgccacac cgtggcagcc ccttcttctc gtttgtagcg ccaggcggaa tagttctcac
    35341 ccacctgcca ttgcgggtag taaggcggtg cggtaatgac ccgcacctca tgaccttgtg
    35401 ccgccaacca ttccaccatc tcgccggtgt atttgccgat gccggttaac tccggcgagt
    35461 agttaatgcc gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag
    35521 aaaataggct cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag
    35581 ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc tcttcttccg ctactctgaa
    35641 gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata
    35701 aaaatgctgc cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc
    35761 cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg tctttctgca cgcgccctcc
    35821 cggcacaaac cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcgcc
    35881 gcgactgttc tcgacaataa agtcgagaga gacaagcggt gtggagcgca ctacagtggc
    35941 aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc ggtcttgatt ctcaaggaac
    36001 cactggtaag tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga
    36061 tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa
    36121 accacccgac ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg
    36181 cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg gttgggtatt ctccagccag
    36241 acttcatgcg ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg
    36301 aattcacgca tcggtgtacc gctgccccat accaccacgt ccgtgccgtc gcctcgtgga
    36361 agcgacgcag caatgctggt atcacatggg agttactcgg gtggaagttg tcatgcggcc
    36421 cgtacaggtt ggtcggcatg acagtgcggt aatcgcgtcc atactggcgg ttgtaagatt
    36481 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    36541 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatgcagg
    36601 acgatccgag aaacatcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    36661 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    36721 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    36781 gcacggcgcg gctgtccagc aggttcagtt catcgcgggt acgtaatacc agctccacat
    36841 caccgcgctg ttcgagctgc cgcttgatgg cagaaccgac catcccacaa tggccagcga
    36901 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    36961 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    37021 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    37081 gccgagcagc gtttcaactt ctgccggacg gaagtaacgc ggatcaacgg cgataatcac
    37141 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    37201 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgctgccg ccatttccac
    37261 aaactgacgt acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    37321 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    37381 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    37441 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    37501 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    37561 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    37621 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    37681 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    37741 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccta ggttgtacac
    37801 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    37861 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    37921 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    37981 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccgg
//
LOCUS       DAJTUN010000025        37805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTUN010000025 DAJTUN010000000
VERSION     DAJTUN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.025"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO470_001212"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO470_001212"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6250523.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO470_001213"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO470_001213"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6250524.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO470_001214"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO470_001214"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6250525.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO470_001215"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO470_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6250526.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO470_001216"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO470_001216"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6250527.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO470_001217"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO470_001217"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6250528.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="OO470_001218"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="OO470_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6250529.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO470_001219"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO470_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6250530.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO470_001220"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO470_001220"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6250531.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO470_001221"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO470_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6250532.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO470_001222"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO470_001222"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6250533.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="OO470_001223"
     CDS             complement(15666..16703)
                     /locus_tag="OO470_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6250534.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="OO470_001224"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="OO470_001224"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6250535.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO470_001225"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO470_001225"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6250536.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="OO470_001226"
     CDS             19445..19885
                     /locus_tag="OO470_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6250537.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO470_001227"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO470_001227"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6250538.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21602)
                     /locus_tag="OO470_001228"
                     /pseudo
     CDS             complement(20612..21602)
                     /locus_tag="OO470_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO470_001229"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO470_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6250539.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO470_001230"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO470_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6250540.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO470_001231"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO470_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6250541.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO470_001232"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO470_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6250542.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO470_001233"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO470_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6250543.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO470_001234"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO470_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6250544.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO470_001235"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO470_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6250545.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO470_001236"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO470_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6250546.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="OO470_001237"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="OO470_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6250547.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO470_001238"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO470_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6250548.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO470_001239"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO470_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6250549.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO470_001240"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO470_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6250550.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO470_001241"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO470_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6250551.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO470_001242"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO470_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6250552.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="OO470_001243"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="OO470_001243"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6250553.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="OO470_001244"
     CDS             37413..37682
                     /locus_tag="OO470_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6250554.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..>37805)
                     /locus_tag="OO470_001245"
     CDS             complement(37685..>37805)
                     /locus_tag="OO470_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2500 family protein"
                     /protein_id="HCR6250555.1"
                     /translation="GGMEQTFRLDAQQYHALTVGDKGTLSYKGTRFVSFVGEQ"
BASE COUNT         9029 a        10513 c         9476 g         8787 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcgc atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgca gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgacaca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat aatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaacg gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcaacac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag catgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg ggagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggctagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaagatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcga gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gctcttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcaggttaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaatagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctggcgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccg
//
LOCUS       DAJTUN010000026        36880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTUN010000026 DAJTUN010000000
VERSION     DAJTUN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.026"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(127..1011)
                     /gene="yhdJ"
                     /locus_tag="OO470_001246"
     CDS             complement(127..1011)
                     /gene="yhdJ"
                     /locus_tag="OO470_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6250556.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1097..1393)
                     /gene="fis"
                     /locus_tag="OO470_001247"
     CDS             complement(1097..1393)
                     /gene="fis"
                     /locus_tag="OO470_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6250557.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1419..2384)
                     /gene="dusB"
                     /locus_tag="OO470_001248"
     CDS             complement(1419..2384)
                     /gene="dusB"
                     /locus_tag="OO470_001248"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6250558.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2713..3594)
                     /gene="prmA"
                     /locus_tag="OO470_001249"
     CDS             complement(2713..3594)
                     /gene="prmA"
                     /locus_tag="OO470_001249"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6250559.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3606..5057)
                     /gene="panF"
                     /locus_tag="OO470_001250"
     CDS             complement(3606..5057)
                     /gene="panF"
                     /locus_tag="OO470_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6250560.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5047..5289)
                     /locus_tag="OO470_001251"
     CDS             complement(5047..5289)
                     /locus_tag="OO470_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6250561.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5398..6747)
                     /gene="accC"
                     /locus_tag="OO470_001252"
     CDS             complement(5398..6747)
                     /gene="accC"
                     /locus_tag="OO470_001252"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6250562.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6758..7228)
                     /gene="accB"
                     /locus_tag="OO470_001253"
                     /gene_synonym="fabE"
     CDS             complement(6758..7228)
                     /gene="accB"
                     /locus_tag="OO470_001253"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6250563.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8206..9180)
                     /locus_tag="OO470_001254"
     CDS             complement(8206..9180)
                     /locus_tag="OO470_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6250564.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9332..11272
                     /gene="csrD"
                     /locus_tag="OO470_001255"
     CDS             9332..11272
                     /gene="csrD"
                     /locus_tag="OO470_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6250565.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11577..12620
                     /gene="mreB"
                     /locus_tag="OO470_001256"
     CDS             11577..12620
                     /gene="mreB"
                     /locus_tag="OO470_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6250566.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12686..13789
                     /gene="mreC"
                     /locus_tag="OO470_001257"
     CDS             12686..13789
                     /gene="mreC"
                     /locus_tag="OO470_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6250567.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13789..14277
                     /gene="mreD"
                     /locus_tag="OO470_001258"
     CDS             13789..14277
                     /gene="mreD"
                     /locus_tag="OO470_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6250568.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14286..14879
                     /gene="yhdE"
                     /locus_tag="OO470_001259"
     CDS             14286..14879
                     /gene="yhdE"
                     /locus_tag="OO470_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6250569.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14869..16338
                     /gene="rng"
                     /locus_tag="OO470_001260"
     CDS             14869..16338
                     /gene="rng"
                     /locus_tag="OO470_001260"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6250570.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16406..20206
                     /gene="yhdP"
                     /locus_tag="OO470_001261"
     CDS             16406..20206
                     /gene="yhdP"
                     /locus_tag="OO470_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6250571.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20362..21807
                     /gene="tldD"
                     /locus_tag="OO470_001262"
     CDS             20362..21807
                     /gene="tldD"
                     /locus_tag="OO470_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6250572.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21935..22864)
                     /gene="aaeR"
                     /locus_tag="OO470_001263"
     CDS             complement(21935..22864)
                     /gene="aaeR"
                     /locus_tag="OO470_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6250573.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23047..23250
                     /locus_tag="OO470_001264"
     CDS             23047..23250
                     /locus_tag="OO470_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6250574.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23258..24190
                     /gene="aaeA"
                     /locus_tag="OO470_001265"
     CDS             23258..24190
                     /gene="aaeA"
                     /locus_tag="OO470_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6250575.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24196..26163
                     /gene="aaeB"
                     /locus_tag="OO470_001266"
     CDS             24196..26163
                     /gene="aaeB"
                     /locus_tag="OO470_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6250576.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26255..26527
                     /locus_tag="OO470_001267"
     CDS             26255..26527
                     /locus_tag="OO470_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6250577.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26583..26846)
                     /gene="yhcN"
                     /locus_tag="OO470_001268"
     CDS             complement(26583..26846)
                     /gene="yhcN"
                     /locus_tag="OO470_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6250578.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27211..27681)
                     /gene="argR"
                     /locus_tag="OO470_001269"
     CDS             complement(27211..27681)
                     /gene="argR"
                     /locus_tag="OO470_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6250579.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28116..29054
                     /gene="mdh"
                     /locus_tag="OO470_001270"
     CDS             28116..29054
                     /gene="mdh"
                     /locus_tag="OO470_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6250580.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29116..30183)
                     /gene="degS"
                     /locus_tag="OO470_001271"
     CDS             complement(29116..30183)
                     /gene="degS"
                     /locus_tag="OO470_001271"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6250581.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30273..31620)
                     /gene="degQ"
                     /locus_tag="OO470_001272"
                     /pseudo
     CDS             complement(30273..31620)
                     /gene="degQ"
                     /locus_tag="OO470_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31774..32172)
                     /locus_tag="OO470_001273"
     CDS             complement(31774..32172)
                     /locus_tag="OO470_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6250582.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32366..33493
                     /gene="zapE"
                     /locus_tag="OO470_001274"
     CDS             32366..33493
                     /gene="zapE"
                     /locus_tag="OO470_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6250583.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33713..34141
                     /gene="rplM"
                     /locus_tag="OO470_001275"
     CDS             33713..34141
                     /gene="rplM"
                     /locus_tag="OO470_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6250584.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34157..34549
                     /gene="rpsI"
                     /locus_tag="OO470_001276"
     CDS             34157..34549
                     /gene="rpsI"
                     /locus_tag="OO470_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6250585.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34944..35582
                     /gene="sspA"
                     /locus_tag="OO470_001277"
     CDS             34944..35582
                     /gene="sspA"
                     /locus_tag="OO470_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6250586.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35588..36085
                     /gene="sspB"
                     /locus_tag="OO470_001278"
     CDS             35588..36085
                     /gene="sspB"
                     /locus_tag="OO470_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6250587.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36128..>36880)
                     /gene="dcuC"
                     /locus_tag="OO470_001279"
     CDS             complement(36128..>36880)
                     /gene="dcuC"
                     /locus_tag="OO470_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6250588.1"
                     /translation="ISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPV
                     MPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFD
                     GMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICA
                     IVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGI
                     AGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8903 a         9225 c         9721 g         9031 t
ORIGIN      
        1 cgaagacagc gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat
       61 gtttaaccaa gtctggcagg aaatatacac gaaatgaata atctgaaaac taggcttaca
      121 cttcctttac tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag
      181 gttgccggtc tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac
      241 atccagccgt cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt
      301 tcgtccgctg gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac
      361 gatatcgcct gggttggaag aggcgagaat aatgcgtttc agtaaggctt tcggtttttg
      421 cgtcgggtgg ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt
      481 acccggtact ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc
      541 gcgctgcgat ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt
      601 tgcgtctttc accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg
      661 tactccagaa ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca
      721 ctggagatcg ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt
      781 tttcagaacg cggtggcact ctgcaatcac ttcaaacagc cagtcgataa acagatcttc
      841 tttccaggct tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa
      901 gagcagatcg acgctttcag taggtagctt tttaagttcg gcaaaggcat caccgtgaat
      961 aatggtctta gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc
     1021 ctttgtgaca cctataaaaa aggcgcttcc ccataccgag tagcgccttt ttaatcaagc
     1081 atttagctaa cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac
     1141 ggttgatgcc catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca
     1201 tgtccaacag gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct
     1261 gaccattcag ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt
     1321 tttgggttac ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc
     1381 gttgttcgaa catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat
     1441 gcctccaacg cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac
     1501 tggtcatttg gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac
     1561 ccttttgccg gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta
     1621 acctctgcca aaggcagtgg gggcagcaac tccccagtgt ccagataatg ctggatttcc
     1681 cgaaagatcc agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta
     1741 tagtcgagca cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga
     1801 atggaaactt tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa
     1861 caggcgcgtg tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg
     1921 gcaatctctt cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca
     1981 ggaacgtcca ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc
     2041 aagagggctg aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata
     2101 tcaataatct gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga
     2161 gcgctaccag caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa
     2221 cgagatttgt cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat
     2281 ccggctccca tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc
     2341 gctgcgatca ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc
     2401 atactgtgac tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac
     2461 tttgaccaat cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgcgataa
     2521 ttactcataa aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt
     2581 gagtaagttc tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata
     2641 atcggcaaat ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg
     2701 gcgatgcgaa ggtcaattct tacgaccggt aatacggcac cactcttctt tttccacgac
     2761 cgggtccagt gcgaagctat cggcataagc ttcacaaacg ctctctgcct ggcttgccag
     2821 aataccggaa aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag
     2881 ttcacgtaat gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg
     2941 ctgatctttc ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttatc
     3001 gcggctggcc tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag
     3061 tttcagcgcc gcgatcgcca gaatgccgga accacagcca aagtcgatga ctgttttacc
     3121 ggttaaatcg aggctgtcga gccattgcag gcacagagag gtggttggat gggtacccgt
     3181 accaaacgcc agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg
     3241 ccagctaggg cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca
     3301 ttcgcgctcc cagtctttat cttctagttg ttcgatttta tgcgcgaagc ctgcgccgag
     3361 cagcggatgg ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag
     3421 accaatcaca tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg
     3481 cgtatcgtgg gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct
     3541 aagatcttcc gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa
     3601 actctttatt tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt
     3661 ccgaccagga aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg
     3721 tactgaatat tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt
     3781 agcgcgcctt tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa
     3841 acggcttcca gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc
     3901 caggcggcaa gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc
     3961 agacgcgtct cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc
     4021 gtagcggaac tttgcagcag ttgggcgtta attgtcgaca tgatcgcagc cataggtgca
     4081 gccagaaaga tcccggcagc aaaaggtggc agcactttta ccattaacgt cgggatcacc
     4141 agatccggta cggtgagatc ggggatcact gcacgaccta acgctccggc caggtgcata
     4201 ccgaacatca ggattgcgac tataatcgta ccgatgatga tcccccggtg tactgctttg
     4261 ctgtctttat aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac
     4321 accagtaccc agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc
     4381 gtaaccagtt gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg
     4441 gcatgtacca cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg
     4501 gtgtcgttca gcacgctggc gcgaaagcca ccaaaggcgg tatataacgc aatgctgata
     4561 ccaaaaatca gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca
     4621 ccgccgataa actgcacggt catcgcacca acgaacgcaa ccagcaaact caaactcgcc
     4681 agccacacca gaagacgact ctggtagcgg gcaaacagca tatcgttcag cgtcactgca
     4741 ttgtagcggc gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagaca
     4801 gcaggaagct gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct
     4861 ggcccgccga taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca
     4921 ataccgccca tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt
     4981 ttacgcatcg cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga
     5041 attacttcaa gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat
     5101 cgcccagcac agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg
     5161 cggaaagccg gtaaaaccgg gggcaacgcc aggtaagtaa gcgtctacta accaaactgc
     5221 cagatacaaa agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg
     5281 agtgtccatt ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg
     5341 tcgatcccca gtaataaaaa aggccggaaa atccggcctt ttgacgcttt tgcagtctta
     5401 tttttcctga agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa
     5461 gttctcgtca ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat
     5521 cagctcctgc agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca
     5581 aatcagctta ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg
     5641 agactcccaa cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct
     5701 tggcaggaag gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac
     5761 gtgaacttct tcttgcttga tcgacagcgg ttgaccggca gcgatacgca gctgttcttt
     5821 gatcaggtca acgccggtga tcatttctgt aaccgggtgt tctacctgaa tacgggtgtt
     5881 catttcgatg aaatagaact cgccgttttc gaacaggaac tcgaaagtac ctgcaccgcg
     5941 atagccgata tcaacacacg ctttagcgca acgtttgccg atgtagcgac gcagttccgg
     6001 ggtaatgccc ggtgctggtg cttcttcgac cactttctgg tggcggcgct gcatggagca
     6061 gtcacgttcc gccagataga tagcgttgcc ctgaccgtca gccagtacct gaatctcgac
     6121 gtggcgagga ttctccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt
     6181 cgcttccgca cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg
     6241 cataccgcga ccgccgccgc cgccggaggc tttgataatc accggataac caatgcgttt
     6301 agcaatggcg cggtttttat ccatatcgtc gcccagcggg ccgtcagaac caggtacgca
     6361 aggaacgccc gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat
     6421 ggtttctgct ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt
     6481 ggcgttctcg gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc
     6541 ggcgctgatg attgccggga tgttcagata actttttact gacggagcag ggccaataca
     6601 gaccgtttca tctgccagta atacgtgttt tagatcgcga tccgtgctgg agtgcacagc
     6661 gacagtcttg atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg
     6721 gttggcaata acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct
     6781 cgtcaaattc taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt
     6841 ccgcttcgat ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt
     6901 tgactttctg acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag
     6961 taccaaccat cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca
     7021 tggaaggaac ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg
     7081 cagcgtaagc ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta
     7141 ctgactcttc gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga
     7201 tcagtttttt aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat
     7261 tgtgacaggc gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg
     7321 ataaattcca gcaggccggg acgccggtct attttgcccc agagtgtcaa tgttagactt
     7381 gacggacatt gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt
     7441 tgcgccaatg tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca
     7501 ttataacgat ttcgtagcaa ttggcagcta aatactggtc ttatcaggga atataatcaa
     7561 cagctaacat gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg
     7621 aaattagcgc catcatcgac ggaacttctt ataacgtaag gtgaaaagct gaaaagccag
     7681 ccctgcgaag atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga
     7741 tgccaggatc gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt
     7801 tcactgcatc gtctggatag atgtgaatgc taaagcacgc aaaatcaacc aactgacaat
     7861 tctcagcatt aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac
     7921 cccatggtac cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata
     7981 aacctaacag gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat
     8041 caatttgtgg tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg
     8101 accaaggtgt tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa
     8161 tgatgtcaaa atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac
     8221 cagcgtgcga ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga
     8281 cagagatatc tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg
     8341 ctgccaggct tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg
     8401 gacattacgc agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc
     8461 acaggccgcc acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc
     8521 gccaacagtg tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa
     8581 ctcatcacga gggagaatac cgctggcacc taaacttttc agatattcat gggtactttc
     8641 gcgaccggaa acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact
     8701 gccgacgcca ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc
     8761 atcttccagc gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc
     8821 tttacgcgcg tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg
     8881 ctccgccagc ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg
     8941 accggcatga aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg
     9001 aatcatcgga aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata
     9061 gttcaggctc gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gacgttcgtc
     9121 cagagtctgt actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat
     9181 aagtggtcct catgtgcatg atggattgga aaataatttc tgaagactat actcgctaat
     9241 ggaaaagcaa agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc
     9301 ccgcttcctc actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt
     9361 tgttacgctg ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt
     9421 ctacaacgcc attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga
     9481 tacccacctt gtgtcgaatg acttcagcgc attaaggcca caaattaccg aattaatggt
     9541 gtcagcagat atcgttcgtg tagacctgct gcatggtgat aaacaggttt ataccctggc
     9601 cagaaatggt agttatcgtc cggttggcac cagcgatctg tttcgtgaac tgagcgttcc
     9661 gttgataaag catccgggga tgtcgttgcg tctggtttat caggatccga tgggcaacta
     9721 tttccattcg ttgatgacca ccgcgccgct cacgggggcg attggcttta tcattcttat
     9781 gctctttctg gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac
     9841 ccgggctact cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga
     9901 atggccgccc agaaccagca gtgcgctgga tacgctgctt cgtgaaattc agaacgcacg
     9961 cgaacaacac agccgtcttg atacgctgat ccgctcttat gccgcacagg acgtgaaaac
    10021 cggcctcaat aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga
    10081 gaaagtaggt acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag
    10141 cgatacctgg gggcacagcc aggttgaaga acagttcttc actctgacga atctgctgtc
    10201 gacatttatg atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc
    10261 tgcgctgtta ccgcaccgga cgttaaaaga ggcagagagc atagccagtc agttaatcaa
    10321 agccgtcgat accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg
    10381 tatctgtgcc tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc
    10441 cacgcgtaat gcgggattgc agggtggcaa tagctgggct atttatgatg actcgttgcc
    10501 tgaaaaagga cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg
    10561 cggcccgcgc ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga
    10621 actcatgtgc cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat
    10681 ggtcttgcag tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc
    10741 actattgcgt tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat
    10801 tcgtccgcgt tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg
    10861 aaaacgcata attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca
    10921 acctgttatt cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt
    10981 gacgctggta agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc
    11041 ggggctggtc aggaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt
    11101 ggaagcctgc tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga
    11161 gtggcagacc ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc
    11221 acagccactt gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt
    11281 ttaatccgtt ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct
    11341 cgccagattg ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag
    11401 gaatgctgct gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac
    11461 gaactgcact aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg
    11521 ttggtaaagt aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt
    11581 tgaaaaaatt tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata
    11641 ccctcattta tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc
    11701 gtcaggatcg tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga
    11761 tgctgggccg tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg
    11821 ccgacttctt cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca
    11881 gctttatgcg tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg
    11941 aacgccgcgc aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg
    12001 aagaaccgat ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta
    12061 tggtggttga tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg
    12121 tttactcctc ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg
    12181 tgcgtcgtaa ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa
    12241 tcggttcggc ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg
    12301 cagaaggtgt tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg
    12361 aaccgctgac cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac
    12421 tggcttccga catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta
    12481 accttgaccg tttgttaatg gaagaaaccg gcattccagt cgttgttgct gaagacccgc
    12541 tgacctgtgt ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg
    12601 acctgttcag cgaagagtaa tcggatgcag gcaggggaag cgtctgttta ccctgcctgg
    12661 tctgatacga gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca
    12721 gattcgcctt attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct
    12781 ggggacgttc agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt
    12841 ttccaatgct cctcgtgaat tgctggatgg catatcgcag acgctggcct cgcgtgacca
    12901 actagaactt gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct
    12961 gatgcttgga caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct
    13021 gcgtcaggat gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag
    13081 cgatcaagtt gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat
    13141 cagcgacaaa ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct
    13201 gctgatttgt gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt
    13261 aattgcagcc ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac
    13321 ggatattcgt gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg
    13381 ctatccggtc gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat
    13441 ccaggcgcgt ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc
    13501 ggatcgtaac ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg
    13561 tctgatgcag atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc
    13621 tgaaccggca acggggatcg ctcagccgac tccgcagcaa ccggcgacag gaaatgcagc
    13681 tactgcgcct gttgcgccga cacagcccgc tgctaatcgc tctccacaaa gggctacgcc
    13741 gccgcaaagt ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat
    13801 cgtagccagg gacgctgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc
    13861 atgccctggc cggataacct gattgttttc cggccaaact gggtgttact tatcttgttg
    13921 tattggatcc tggccttgcc ccatcgcgta aatgtgggca caggttttgt gatgggtgcc
    13981 atactggatc tgatcagcgg ctcgatgctt ggcgtacgcg tattggcgat gagcatcatt
    14041 gcttacctgg tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg
    14101 ctggtcgtca tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta
    14161 gtgattaacg tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc
    14221 tggccgtgga ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg
    14281 tttctatgac ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc
    14341 aacttggcgt gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg
    14401 agagcgcgca gcagtatgtt gtgcgtctgg cacgcgagaa agcacaggcg ggtgtcgcgc
    14461 aaacggcaca ggatctcccg gtgctgggtg cggatattat cgttatcctg aacggagaag
    14521 tgctcgagaa accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc
    14581 agacccatca ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgtc
    14641 tggtggtcac cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg
    14701 ccagcggtga accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt
    14761 ttgtcaggaa gataaatggc agctatcacg ccgtagtcgg cttaccgctg gttgaaacgt
    14821 atgaattatt aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa
    14881 ttgttagtaa acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg
    14941 caggaaattc atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt
    15001 cgtgtaagtc gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa
    15061 gccgcgtttc ttcatgcatc cgacatcatg ccgcataccg aatgtgtggc gggtgaagaa
    15121 caaaagcaat tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg
    15181 gtgcaggtgg tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg
    15241 ctgccttctc gctatctggt atttatgcca ggggcttctc acgttggggt ttcccaacga
    15301 attgaaagcg aatcagaacg tgaacgtctg aaaaaagtgg tcgcagagta ttgcgacgag
    15361 cagggcgggt ttatcatccg caccgcagcg gaaggggttg gcgaggctga actggcctcc
    15421 gatgccgctt atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag
    15481 acccgttatc agctgtacgg cgaactggcg ctggcgcagc gtgttctgcg tgattttgcc
    15541 gacgccgaac tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attgctggag
    15601 tttacctcgg agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag
    15661 ccgattttcg atctctttga tgtcgaaaac gaaatccagc gagcgttgga acgcaaagta
    15721 gaactgaaat ccggtggtta tctgattatc gaccagaccg aagcgatgac caccgtggac
    15781 atcaataccg gagcgtttgt cggtcatcgc aatctggacg acactatttt caataccaat
    15841 attgaagcga cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc
    15901 attattgatt tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcattcgctg
    15961 gagcaggcgt tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg
    16021 ctggtggaga tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa
    16081 tgcccaacct gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc
    16141 atgcgcgaga ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct
    16201 tctccggcag tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa
    16261 attttcgttg gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt
    16321 gacgtcgtaa tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac
    16381 atttttagca gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg
    16441 agccgcgctc gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca
    16501 tcttaacgcc tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt
    16561 agaagccagt cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga
    16621 catccgtgca gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct
    16681 ggatgtctgg cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca
    16741 gctgcgcttt cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc
    16801 cagtcacatc agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga
    16861 agtcagtttt ctgacgccat ccggtcagcg cgccgagctg gcgatcccac aactcacctg
    16921 gctgaacgat ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg
    16981 acagcacggc gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa
    17041 tggtcgcgtc tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat
    17101 gcaggacaat attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga
    17161 caaaggcgat gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg
    17221 cgagaagcaa acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa
    17281 tccgggctgg cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc
    17341 gagcggagca ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa
    17401 acgcagtgac gaactccgga ttcgcgctag taatctggag ctggcaggcc tggagggcat
    17461 acgcccgctg gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc
    17521 gagcggcaag attaacactc tggcgctgga tatcccgctt caggcggcag gcaagacccg
    17581 ttttcaggca tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga
    17641 acacttctcc gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa
    17701 gcaggcaaag atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca
    17761 ggcaactata agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga
    17821 cgttaaagcc aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga
    17881 tgaaccctgg ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggcg
    17941 ctatttcccg gaaaacttga tgggtaaaga cttggttgat tatttaagtg gtgcgattca
    18001 gggcggtgaa gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta
    18061 taaacacaac gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt
    18121 ccagccggac tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg
    18181 tttatggatg aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc
    18241 agtgatccct gactactcaa aagaaaaact gctgattgac gctgacatta aaggtccggg
    18301 taaagccgtt ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct
    18361 gcaagaactc cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa
    18421 cggcgaactg gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa
    18481 accactcgac agcatcctga aaaatttgag cggtaaattc agctttatca atggcgatct
    18541 gcaaagtgaa ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac
    18601 caaagaaggg gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc
    18661 gaaaaccggc gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga
    18721 tggtaaagtg ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa
    18781 cggcgatctg aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga
    18841 accactagcg gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca
    18901 ggctggtgcg gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga
    18961 tcgtgctatt tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt
    19021 tgaacttaat atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc
    19081 tgcggagagt gtcggtggtg cagcgagttt cccacaacac ataacgttac gtacgcctat
    19141 gttgtcactg ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa
    19201 tggcaccctg gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa
    19261 cgcgccgtgg ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg
    19321 tggtgattca acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg
    19381 gccggacgca caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat
    19441 tgacagtgat atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac
    19501 tggtttctcg cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac
    19561 ctcgctgaaa ggaaaactgc gtgggcagaa aattgatgcc gccgcagaat tttttggtgt
    19621 cacgacgccc atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc
    19681 accctggcag ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg
    19741 cgaaattacc gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga
    19801 tgccctgatg cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt
    19861 tgactccatt cgcagcaccg cgtggattaa agacggcgtt atgcacaccg acgacacgct
    19921 ggtggatggc ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgccgcga
    19981 cctgaatatg gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc
    20041 ttttgcggtt aaccccattg ttggcgcggc agtgtttgcc gccagtaaag tgctggggcc
    20101 gctgtggagc aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca
    20161 aatcaacgaa gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc
    20221 gtaattgccc caatctcata tgataatcgt tgccaaaggc caacgagccg gaacataact
    20281 gtaggccgga tagggcgttc acgccgcatc cggcagccgt aaaaaatcct ctactgcagt
    20341 aactaacgag tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg
    20401 aacggcctga aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt
    20461 gattatggcg atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc
    20521 attattaaag atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt
    20581 gaaaaaaccg gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg
    20641 caagcggcgc gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg
    20701 gtagagcata gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag
    20761 aagctggata tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag
    20821 gaagtgactg ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc
    20881 acgctagcgg cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa
    20941 gatggcaaac gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc
    21001 cttgccgatc ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg
    21061 gcgctggtca atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc
    21121 gcaggttggc cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc
    21181 aaccgccgtg gcacttcagt atttagtgga caggtcgggg agctggtggc ttcagaactg
    21241 tgtaccgtgg ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgat
    21301 gaaggtacgc caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg
    21361 caggataaac tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa
    21421 tcctacgccc atctgcccat gccgcgtatg accaacacct atatgctgcc gggtaaatcg
    21481 accccgcaag aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc
    21541 ggtcaggtgg atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt
    21601 gaaaacggta aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa
    21661 accatgcagc agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc
    21721 tgcggtaaag aagggcaaag tttgccggtt ggcgtgggcc agccaacgtt gaaagtcgat
    21781 aacctgactg ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct
    21841 ttctacgtgc acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg
    21901 cacaggtttg tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca
    21961 tctcctgaaa caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct
    22021 gtaccttcag cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg
    22081 actgataacg cggcagcagg atctccagct ccccacgatt gatctcgttg atcacccaca
    22141 tcagcggcac gtaggcgatc ccggcacccg ccgttagcca gcgcaccagc gtcatcggat
    22201 cattagtcac aaatctccct tgcgggatca ggcgagtcga gatcccttcc ggtgcaatca
    22261 gttcaaattc attgtcgggc cgcacgctgt attcaagcca tgaatggcta cttaaatcgg
    22321 cgggtttttc cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccattg
    22381 gcatcgcgcc aagacggcgg gaaaacaggc tggaatcctg caacgcgccg acgcggatca
    22441 ccacatccag accgtcggca atcaggtcgg gggctggaat tccggtaacc agattggcgc
    22501 tcaaacctgg gtattctttc agcattttgg ctgtcagccc ggcgagaaca ttttgtgcca
    22561 tagttgaaga acagccaatg cgtagcgtcc cgatgggggt gttattgaag gcatacagtt
    22621 gctcatgaac atcctgcact tcatgaagca tacgacggca gccctggtag taaattctac
    22681 cggcttcggt caggccaatg ctgcgtgtgc tacggtttaa cagctttacc tgcaactcat
    22741 cttccagttt tgacaccgtc tgactgatgg acgaaacgct catctgtagc tgtctggcgg
    22801 cggcggtaaa agagccaaat tcaactactt tggcaaacac cgacatgcgt tttagtcgtt
    22861 ccattattca ctctgactta aaagtgattt agatcacata atatagataa cagcataaca
    22921 gttacgctaa tatattaaat atcaatctac agcaatgttg ctctcgcccg gctttccatg
    22981 cccttctctg cggcgacaga tgctgaaaat aataacgcct gctctctcta tacaaccaag
    23041 gtcaacatga gtctgtttcc cgttatcgtg gtgtttgggc tgtccttccc accgatattt
    23101 tttgaattgc ttttatcact ggcgattttc tggctggtgc gccgggtact tgtgccaaca
    23161 ggtatctacg actttgtctg gcatccggcg ttgttcaaca ccgcgctcta ttgctgcttg
    23221 ttttatttga tatcgcgact gttcgtttga ggttgaagtg aaaacactaa taagaaaatt
    23281 ctcccgtacg gccatcacgg tcgtattagt cattctggcc ttcatcgcaa tttttaatgc
    23341 ctgggtctat tacaccgaat ccccctggac gcgtgacgcg cgctttagcg ctgacgtcgt
    23401 tgcgatcgcg ccggacgttt ctggactcat tacccaggtg aatgttcatg ataaccagct
    23461 ggtgaaaaaa ggacagatac tgttcaccat cgaccagccg cgctatcaaa aggcgcttga
    23521 ggaagcgcaa gccgatgttg cttattatca ggtactggca caggagaaac gccaggaggc
    23581 cggacgtcgt aaccgtctcg gtgtgcaggc gatgtctcgc gaagagatcg accaggctaa
    23641 caacgtacta caaacggttc tgcatcagtt agcgaaagcg caggcgaccc gcgatctggc
    23701 aaaactggat cttgaacgca cggtgatccg agcgccagca gatggctggg tgaccaacct
    23761 caacgtctat accggtgagt ttattactcg aggatcaacg gcggttgcgc tggtgaaaca
    23821 gaactccttc tatgtactgg cctatatgga agaaactaag ctggaagggg tgcgtccggg
    23881 gtatcgtgca gagatcacgc cgcttggcag taacaaagtg ctgaaaggga ctgttgatag
    23941 tgttgccgct ggggtcacca acgccagcag cacgcgtgac gacaaaggga tggcgaccat
    24001 cgactctgac cttgaatggg tgcgtttggc gcaacgtgtt ccggttcgta ttcgtctcga
    24061 caaccagcaa gagaacatct ggcctgcggg caccactgct acagtggtgg tcactggcaa
    24121 acaagatcgc gacgaaagcc aggattcgtt cttccgtaaa atggcccatc gcctgcgtga
    24181 gtttggttaa tcacgatggg tattttctcc attgctaacc aacatattcg ctttgcggta
    24241 aaactggcga ccgccattgt actggcgctg tttgttggct ttcacttcca gctggaaacg
    24301 ccacgctggg cggtactgac agcggcgatt gttgccgccg gtccggcctt tgctgcggga
    24361 ggtgaaccgt attctggcgc tattcgctat cgtggctttt tgcgcatcat cggcacattt
    24421 attggctgta ttgccggact ggtgatcatc attgcgatga tccgcgcacc attattgatg
    24481 attctggtgt gctgtatctg ggccggtttt tgtacctgga tatcctcgct ggtacgaata
    24541 gaaaactcgt acgcgtgggg gctggccggt tataccgcgc tgatcattgt gatcaccatt
    24601 cagccggaac cattgcttac gccgcagttt gccgtcgaac gttgtagcga gatcgttatc
    24661 ggtattgtgt gtgcgattat ggcggatttg ctcttttctc cgcgatcgat caaacaagaa
    24721 gtagatcgag agctggaaag tttgctggtc gcgcaatatc aattaatgca actctgtatc
    24781 aagcatggcg atggtgaagt tgtcgataaa gcctggggcg acctggtgcg acgcaccacg
    24841 gcactacaag gcatgcgcag caacctgaat atggaatctt cccgctgggc gcgggccaat
    24901 cgacgtttaa aagcgatcaa tacgctatcg ctgacgctga ttacccaatc ctgcgaaact
    24961 tatcttattc agaatacgcg cccggaattg atcactgata ctttccgcga attttttgac
    25021 acgccggtag aaaccgcgca ggacgtccac aagcagctca aacgcctgcg gagagttatc
    25081 gcctggaccg gggaacggga aacgcctgtc accatttata gctgggtcgc ggcggcaacg
    25141 cgttatcagc ttctcaagcg cggcgttatc agtaacacaa aaatcaacgc caccgaagaa
    25201 gagatcctgc aaggcgaacc ggaagtaaaa gtagagtcag ccgaacgtca tcatgcaatg
    25261 gttaacttct ggcgaaccac actttcctgc attctgggca cgcttttctg gctgtggacg
    25321 ggctggactt ccggcagtgg tgcaatggtg atgattgcgg tcgtgacgtc actggcaatg
    25381 cgtttgccga atccacgcat ggtggcgatc gactttatct atgggacgct ggccgcgctg
    25441 ccgttagggc tgctctactt tttggtgatt atccctaata cccaacagag catgttgctg
    25501 ctgtgtatta gcctggcagt gctgggattc ttcctcggta tagaagtaca gaaacggcga
    25561 ctgggctcga tgggggcact ggccagcacc ataaatatta tcgtgctgga taacccgatg
    25621 actttccatt tcagtcagtt tctcgacagc gcattagggc aaatcgtcgg ctgtgtgctc
    25681 gcgttcaccg ttattttgct ggtgcgggat aaatcgcgcg acaggactgg acgtgtactg
    25741 cttaatcagt ttgtttctgc cgctgtttcc gcgatgacta ccaatgtggc acgtcgtaaa
    25801 gagaaccacc tcccggcact ttatcagcag ctgtttttgc tgatgaataa gttcccaggg
    25861 gatttgccga aatttcgcct ggcgctgacg atgattatcg cgcaccagcg cctgcgtgat
    25921 gcgccgatcc cggttaacga ggatttatcg gcgtttcacc gacaaatgcg ccgcacagca
    25981 gaccatgtga tatctgcccg tagcgatgat aaacgtcgtc ggtactttgg ccagttgctg
    26041 gaagaactgg aaatctacca ggaaaagcta cgcatctggc aagcgccacc gcaggtgacg
    26101 gaaccggttc atcggctggc ggggatgctc cataagtatc aacatgcgtt gaccgatagt
    26161 taagtcaaaa ccgacgccaa aagcgtcggt tttttcatgg ctatacttag cgatgcacgg
    26221 cagaactcgc cgcgaaacgt gacggtggca acagatgaat atttatgcct ttgattttga
    26281 tgagattgag agtcaggagg atttttatcg tgactttagc caaacctttg gtctggctaa
    26341 agataaggta cgcgatctcg actcactatg ggatgtgtta atgaacgatg tcctgccgct
    26401 accacttgag attgaatttg ttcatctggg agagaaaacg cgtcgccgtt ttggcgcgtt
    26461 aatattgctg tttgatgagg cagaggaaga gctggaaggg catttgcgtt ttaatgttcg
    26521 tcattagcgc aaaaaaaagc ccacgaaccg ggggcaatat cgtcggacaa aacgatgagg
    26581 gtttatttgt acagttcagc cgtagcgtgc caggtgtcac cgctacgagc ttcagtaatc
    26641 tggtaggccg ttgcgccttt ctcttccgct tttttgttca gcatttcacg tatatccatt
    26701 ggcgaagacg ccacaccact tacggatacg gtcccgattg cttcacgatt ttgtgcttgt
    26761 gcagcatcaa tggagtcggc agcgaatgca ccgaaagaga gaacagaaag tacgcttaat
    26821 gcagctacag tggttttgat tttcatgatt tttacctcga cataatcttt tagctgggcc
    26881 tttgtttcgt gacccatatc acaaaatcaa gtatacacta attattgaac taattaatac
    26941 cacactaact gtttcagttg atactaagtg ttaaaaatgt gaattttaat aacaaaaaag
    27001 tataaaaaac acggttattt attattaatt atagtatttt caattagata atgaattttg
    27061 cataatgtgc tttttttacc gtatgtattc attttgtgaa tgatgtgtcg cggtaaaacg
    27121 cactattcgt taaatgtatc gcaggagaaa gaaggggaat gagagggata agcaacattt
    27181 tccccgccgc cagaagcgac ggggcagaga ttaaagctcc tggtcgaaca gctctaaaat
    27241 cgcttcgtac aggtctttga cggtgaaacc gttagcaggg gtggtaaaga tggtgtcatc
    27301 gccagcgatg gtgcccagaa taccttctgc tttgcccagt gagtccagca ggcgagcaat
    27361 taactgcgcc gcgccagggc tggtatgaat cacgacaact gcatcgttgt agtcgatatc
    27421 cagcaccaga ttcttcaatg gactggaggt ggttggtaca cccagttcag ctggcaggca
    27481 gtaaaccatt tccattttgg cattgcgtgt acgtacagca ccaaacttgg tcaacatccg
    27541 cgagacttta gactgattaa tattgtcaaa gccttgctcc tgcaacgcgg cgacgatttc
    27601 gccctgggag ctaaatttct cttctttaag taatgcttta aatgctttaa ctagttcttc
    27661 ttgcttagcc gagcttcgca taagtcaccc gatatggtgg ttgatacaac attattgtgc
    27721 atacagatga atttttatgc aaacagtcag ccctgaagaa ggctgaaata atgttatgaa
    27781 agagcgggat tttatcaaat ttcgttattg agaaacatgc ctgcgtcacg gcatgcaaat
    27841 tctgtttaaa agtaaattaa ttgttatcaa attgatgttg ttttggctga acggtagggt
    27901 atattgtcac cacctgttgg aatgttgcgc taatgcataa gcgactgtta attacgtaag
    27961 ttaggttcct gattacggca attaaatgca taaacgctaa acttgcgtga ctacacattc
    28021 ttgagatgtg gtcattgtaa acggcaattt tgtggattaa ggtcgcggca gcggagcaac
    28081 atatcttagt ttatcaatat aataaggagt ttaggatgaa agtcgcagtc ctcggcgctg
    28141 ctggcggtat tggccaggcg cttgcactac tgttaaaaac ccaactgcct tcaggttcag
    28201 aactctctct gtatgatatc gctccagtga ctcccggtgt ggccgtcgat ctgagccata
    28261 tccctactgc tgtgaaaatc aaaggttttt ctggtgaaga tgcgactccg gcgctggaag
    28321 gcgcagatgt cgttcttatc tctgcaggcg tagcgcgtaa accgggtatg gatcgttccg
    28381 acctgtttaa cgttaacgcc ggcatcgtga aaaacctggt acagcaagtt gcgaaaaact
    28441 gcccgaaagc gtgcattggt attatcacta acccggttaa caccacagtt gcaattgctg
    28501 ctgaagtgct gaaaaaagcc ggtgtttatg acaaaaacaa actgttcggc gttaccacgc
    28561 tggatatcat tcgttccaac acctttgttg cggaactgaa aggcaaacag ccaggcgaag
    28621 ttgaagtgcc ggttattggc ggtcactctg gtgttaccat tctgccgctg ctgtcacagg
    28681 ttcctggcgt tagttttacc gagcaggaag tggctgatct gaccaaacgt atccagaacg
    28741 cgggtactga ggtggttgaa gcgaaagccg gtggcgggtc tgcaaccctg tctatgggcc
    28801 aggcagctgc acgttttggt ctgtctctgg ttcgtgcact gcagggcgaa caaggcgttg
    28861 tcgaatgtgc ctacgttgaa ggcgacggtc agtacgcccg tttcttctct caaccgctgc
    28921 tgctgggtaa aaacggcgtg gaagagcgta aatctatcgg taccctgagc gcatttgaac
    28981 agaacgcgct ggaaggtatg ctggatacgc tcaagaaaga tatcgcccta ggcaaagagt
    29041 tcgttaataa gtaattgatt agcgaataat aaaaaaccgg agcacagact ccggtttttg
    29101 ttttgagcac tcgacttaat tggttgccgg atattcctga atggtgacct gcagcgttaa
    29161 ctgcttatca tcacgcatca ctacaaccgg gatcaccgaa ccagggcgaa tttccgccac
    29221 ctgatccatc gtctccagag cagagatggc cggtttgtta tccaccgaaa taatcagatc
    29281 gttgacctga atacccgcat tcgccgctgg gccgtcaggt gacacttcat taaccacgat
    29341 cccttgcagt tgatctatac caccgctctg cgcgtgcagt ggtgcgatct cgcgtccgcc
    29401 gataccaatg tagccgcgga tcacgcgacc atcgcggatc agcttatcca taattttggt
    29461 tgctaactgg aaaggaatcg caaagccgat accttccggc gtttcgccat cgttactctt
    29521 atcaaacgac agcgtgttaa tgcccatcag ttctcccagc gagttcacca gcgcgccgcc
    29581 agagttaccg tggttaatgg aagcatcggt ttgtaggaag ttttgccgcc cggtcgggtt
    29641 cagaccgatt cgacccgtgg cactaataat cccttgggta atggtctgcc cgaggttgta
    29701 cgggttaccg atcgccagta ctacgtcgcc aatgtgcggt acgcgacgtg cattaattgg
    29761 aatggtaggt aaaccgccag tggcattaat tttaagtacc gccagatcgg ttagagagtc
    29821 agatcccacc agcaatgctt caaatacacg tccatcctgt aaggcgacga tgatctgatc
    29881 ggcgtcgttg atgacgtgtt tattggtgat gatataaccg cgttgatcca tgattacacc
    29941 ggatcccagg gtgcggatct caagctggtt gtgagagttg gtgttcaaac cacggttgta
    30001 aacgttaacc accgctggcg cggcgcggcg aaccgccaga ttatagctgg caggcgtctc
    30061 atcggtactg tcaaattgcg gagtggaaag cgggttaagg ctgcgcagcg aaggcatggc
    30121 aaccagcaga atagcgccga caattaatcc aatcgcaacg gaacgtaaga gcttcacaaa
    30181 catgatggag gcgtcattaa aaaagggaac ggcagcagca taccacgagt taaccggaca
    30241 tcacacgtca gcctgatgcc cggtttacga cattaacgca tcagcagata gatgctttca
    30301 ttgccgcgta caatttgcag ggcgatgatg gccggttttg ccgccagcac tttacgcatt
    30361 tcagcaatcg agttcacccg atcgcggttg acgccaatga tcacatcgtc tttttgcaag
    30421 ccagcctgag cagctgggct tcctttgaca acttcatcta tcttaatacc tttgccgcca
    30481 tcttttagct gaccatcgct caacgttgca ccttccagcg ctggccgacg aagaggtgct
    30541 ggtatcaagc gtcacttcta cttccagtgg tttgccgtta cgcagcaggc caagcttcac
    30601 tttcgtgccc ggctctgtag tcgcgatacg agagcgcaac tcagcaaagc tattcagcgg
    30661 tttgccgttg aggctggtaa taatatcgcc cgctttgacg cccgctttcg ctgagccaga
    30721 acttggcaac acttcgctga caaacgcgcc acgctgcacg tcaaggttga aggctttggc
    30781 gatatcggca ctcatctcgg tgcctttgat gcctaacaaa ccgcgtttga tttcaccaaa
    30841 gtcgataagc tgctgcgcca gtgttcgcgc catattactg gggatggcaa atccaatccc
    30901 gacgctcccg ccgccaggcg caaggattgc agtgttgatg ccaattaact caccgttaag
    30961 gtttaatagt gcaccgccgg agttaccgcg gttaatggaa gcatctgtct ggataaagtt
    31021 ttccagacct tcaagattca acccgctgcg gcctaatgcg gaaacaatgc cagaggtggc
    31081 ggtttgccca aggccaaatg ggttaccgac cgctacggca aaatcaccga cgcgcaattt
    31141 atcggagtcg gcaatagcga tttgcgttaa tttgctcggg ttttgaattt gtaacagggc
    31201 gatatcgctc tggtcatcgc taccaatcag ttttgcatca aactcgcgcc catcattgag
    31261 ctgaatactg attttctgtg cctgattaat cacatggttg ttggtcagca catagccttt
    31321 actggcgttg atgatgacac cggaacctaa accttcgaag ggttgtgcag gttgatccgg
    31381 taaatcatca ccaaaaaact ttttgaattc ttccgggatt ttctgtccct gactggccgt
    31441 tccttccacc cgtacgctca ccactgccgg aagcactttt tccagcattg gagccagact
    31501 ggggagaggg gcctgatcgg caacctggcc tggaatcgac gcgacggcct gaaatgacgc
    31561 cgagagagtt aacccggcac ttaacgctaa tgcactcaac agctgggttt gttttttcat
    31621 tattcctgct ctcgtacctg aatgataaga aaagagattc aaaacagttt gatgttattg
    31681 aattttcaga cggctaacaa tgagacacca gattaaatga tagtcgggga gggagagaag
    31741 aggagggcgc aatggctgcg cccgaaaaat aaattagtcg cgcttcgcgc cagtacgcag
    31801 caggccggat gcgccttcag aatagtcgcg aggcacctgc accggtgcct gatcgttgct
    31861 ggcttcagac tctgccagac gattacggaa cgggtttgct tcagcagaca gttccggcag
    31921 caggctgctg gagctttttg ccatgtgctg atacagctgg cgatagtcgt gcgccatggt
    31981 atccagtaat tccgcgctgc gggcaaagtg gctaaccagc tcttcgcgat actcgtccag
    32041 ttcagcttta ttcttttcca gttcgtactg caacgcctgt tgctggcgta gtttacgatt
    32101 accaaaacgc atggccacag caccaataat gatgccgacg actaacccaa ttagcgcata
    32161 ttcccaggtc atgaacatct cccgttgtct tttgtttccg tagggtgttg gcttcaggct
    32221 ccatgcctgc ggctgattat gccactataa ccgttaattc cacagaagtg gaatcccgac
    32281 tgcatatcgc gtagtgtaga acggcctttt tttcgtcaac cgtgaacaac agcgcaccga
    32341 ttattcaagg aataacaata agatcatgca aagcgttacc ccaacatcgc aatacctgaa
    32401 ggcgctcaat gaaggcagcc atcaacccga cgacgttcaa aaagaggccg tcagccgcct
    32461 ggaaattatt tatcaagaac tcatcaatag caggccacca gcccccagga cgagtgggct
    32521 aatggcgcgg gtcggtaagc tgtggggtaa acgcgaagac acaaagcata cgccagtgcg
    32581 tggcttatat atgtggggcg gtgtaggacg cgggaaaacc tggctgatgg accttttcta
    32641 tcaaagcctg ccgggagagc ggaaacagcg cctgcacttt caccgtttta tgctgcgggt
    32701 gcacgaagag ctaactgcct tacaggggca gaccgatccg ctggaaatta ttgccgatcg
    32761 ctttaaagcc gaaactgacg tgctctgttt tgacgaattt tttgtttctg atattaccga
    32821 cgccatgcta cttggcggtc tgatgaaagc cctgttcacc cgcggtatta ccctggtagc
    32881 gacgtcaaat attccgccgg acgaacttta tcgaaatggc ctgcaacgtg cgcgttttct
    32941 gcctgcaatc gatgccatta aacagcattg tgatgtaatg aacgtggacg ctggtgttga
    33001 ttatcgactg cgtacactca ctcaggcgca tctgtggctt tcgccactca acgatgaaac
    33061 ccgggcgcag atggataaac tatggttggc gctggcgggg gtgaaacgag aaaattcacc
    33121 gacgttagaa atcaaccatc ggccattggc gacaatgggc gtcgagaacc agacgctggc
    33181 ggtttctttt actacgctgt gcgtcgacgc ccgcagtcag catgactata ttgcgctctc
    33241 acgcctgttt cacacagtaa tgttgtttga tgtgccagtc atgacgcggt tgatggagag
    33301 cgaagcgcgg cgctttattg cgctggtgga tgagttttac gagcgccatg tcaaattagt
    33361 ggtgagtgca gaagtgccgc tgtatgaaat ttatcagggt gagcggctga agtttgagtt
    33421 ccagcgttgc ctgtcacgtc tgcaagagat gcaaagcgaa gagtatctga agcgcgagca
    33481 tttggcgggt taaaacctgc cacaaatcac aaaaaggggt cgatctttga ccccgacttc
    33541 tctataatcc tgcgacccca cgttacaaga aagttttttt ccccaaaact ttttgtgtgc
    33601 tggcataggc tattcgaagg ggtaggtttg ccggactttg tcgtgtgaac ctcaacaatt
    33661 gaagacgttt gggtgttcac caacgtgtaa ctatttattg ggtaagcttt taatgaaaac
    33721 ttttacagct aaaccagaaa ccgtaaaacg cgactggtat gttgttgacg cgaccggtaa
    33781 aactctgggc cgtctggcta ctgaactggc tcgtcgcctg cgcggtaagc acaaagcgga
    33841 atatactccg cacgtagata ccggtgatta catcatcgtt ctgaacgctg acaaagttgc
    33901 tgtaaccggc aacaagcgta ctgacaaagt gtactatcac cacaccggcc acatcggtgg
    33961 tatcaaacaa gcgacctttg aagagatgat tgctcgccgt cctgagcgtg tgattgaaat
    34021 cgcggttaaa ggcatgttgc caaaaggccc gctgggtcgt gctatgttcc gtaaactgaa
    34081 agtttacgcg ggtaacgagc acaaccacgc ggcacagcaa ccgcaagttc ttgacatcta
    34141 atcgggatta taggcaatgg ctgaaaatca atactacggc actggtcgcc gcaaaagttc
    34201 cgcagctcgc gttttcatca aaccgggcaa cggtaaaatc gtaatcaacc aacgttctct
    34261 ggaacagtac ttcggtcgtg aaactgcccg catggtagtt cgtcagccgc tggaactggt
    34321 cgacatggtt gagaaactgg acctgtacat caccgttaaa ggtggtggta tctctggtca
    34381 ggctggtgcg atccgtcacg gtatcacccg cgctctgatg gaatacgacg agtccctgcg
    34441 ttctgaactg cgtaaagctg gcttcgttac tcgtgacgct cgtcaggttg aacgtaagaa
    34501 agtcggtctg cgtaaagcac gtcgtcgtcc gcagttctcc aaacgttaat tggcttctgc
    34561 tacggcagaa aacaatttgc gaaaaaaccc gcttcggcgg gtttttttat ggctaaattc
    34621 tgaatcagcg taaaaaatgg gaagttgttt tttgctgccg tctgaaagac aggtaaaaca
    34681 aaaaccacat cgccaataag ggtctaagtc aactatttca gactaaagcg catctctttt
    34741 tccccatttc cggcatccac tcaccacaaa ggtcgcaaaa tctggtaaac tatcatccaa
    34801 ttttctgccc aaatgtcggg tattgctcat tttttgtttg attttcgaac aaagagagtc
    34861 gttccttatt gggtaacaca acttctgact ggccacctgg tggctggtag cagtaaaaat
    34921 tctgactata cctggaggtt ttcatggctg tcgctgccaa caaacgttcg gtaatgacgc
    34981 tgttttccgg tcctactgac atctatagcc atcaggtccg cattgtgctg gctgagaaag
    35041 gtgtaagttt cgagatcgaa cacgtggaaa aggacaatcc gcctcaggat ctgattgacc
    35101 tcaacccgaa tcagagcgtt ccgaccctgg tggatcgtga gctgaccctg tgggaatctc
    35161 gcatcattat ggaatatctg gatgagcgtt tcccgcatcc gccactgatg cctgtttacc
    35221 cggtagctcg cggtgaaagc cgtctgtaca tgcatcgcat cgaaaaagac tggtacacgc
    35281 tgatgaacac tatcatcaac ggttcagctt ctgaagcaga tgccgcacgt aagcaactgc
    35341 gcgaagaact gctggcgatt gcgccggtct tcggtcagaa gccgtacttc ctgagcgatg
    35401 agttcagcct ggtcgattgc taccttgctc cgctgctgtg gcgtctgccg caactgggca
    35461 tcgagttcag cggcccgggt gcgaaagagc tgaaaggcta tatgacccgc gtctttgagc
    35521 gtgactcttt ccttgcttct ttaactgaag cagaacgtga aatgcgtctg ggccggagtt
    35581 aatctgtatg gatttgtcac agctaacacc acgtcgtccc tatctgctgc gtgcattcta
    35641 tgagtggttg ctggataacc agctcacgcc gcacctggtg gtggatgtga cgctccctgg
    35701 cgtgcaggtt cctatggaat atgcgcgtga cgggcaaatc gtactcaaca ttgcgccgcg
    35761 tgctgtcggc aatctggaac tggcgaatga tgaggtgcgc tttaacgcgc gctttggtgg
    35821 cattccgcgt caggtttctg tgccgctggc tgccgtgctg gctatctacg cccgtgaaaa
    35881 tggcgcaggc acgatgtttg agcctgaagc tgcctacgat gaagatacca gcatcatgaa
    35941 tgatgaagag gcatcggcag acaacgaaac cgttatgtcg gttattgatg gcgacaagcc
    36001 agatcacgat gatgacactc atcctgacga tgaacctccg cagccaccac gcggtggtcg
    36061 accggcatta cgcgttgtga agtaatacaa aacaggccca ggcggcctgt tttgtctttt
    36121 taatgactta ataaaatagc gtgattgtgg caatcatatt caccacgaaa ccgactgcca
    36181 tggggatcgc tgtccgcttc accaccgcaa aaggcgaaac gcctgcaatt cctgacgtaa
    36241 cgaccaccac cgcagtaatc ggcgaaaccg cgcgcgctag cgtcgtggca aaatgcatcg
    36301 gcataatcat tacaaccgct ggtacatgta gtccggctgc gatattcgga ataagactgg
    36361 caaatgacat aaacggcgca ttgccagagc ccatcacaat ggcacaaatg gcaatgacta
    36421 gcgccataat aatcatcacg ccaataccgc ccagaccaga atgctccgca cccctgataa
    36481 ccgcatcaac agtgccaatc gtcgttaagc ctttcgcaaa tatttcaccc gcgaccacca
    36541 gcgttaccac gttgacaaac tgcgtaccca tgccgtcaaa aaacgcctgc acatcgtcca
    36601 ttgtttcgcg caagttatgc ttgcggaaga actcaacaaa catcgtcacc gtcaaactta
    36661 gtaacatcac caccaccaga tgcagttctg acttcatcag cccgacgtgg gcgaggaaca
    36721 gcgagccgag catcaggatt aacggcatca caggtaaaat ggcgtaatag agcggcggga
    36781 cattatcgag agttttttgc tctgcctgtt cgtgattgat atttttatct tttttgtcaa
    36841 aagcgcgttg cacgaaaaag tgggagatcg ccaccgagat
//
LOCUS       DAJTUN010000027        36089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTUN010000027 DAJTUN010000000
VERSION     DAJTUN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.027"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..236
                     /locus_tag="OO470_001280"
     CDS             <1..236
                     /locus_tag="OO470_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6250589.1"
                     /translation="RGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRP
                     LIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(283..1041)
                     /gene="deoR"
                     /locus_tag="OO470_001281"
     CDS             complement(283..1041)
                     /gene="deoR"
                     /locus_tag="OO470_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6250590.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1099..1695)
                     /gene="ybjG"
                     /locus_tag="OO470_001282"
     CDS             complement(1099..1695)
                     /gene="ybjG"
                     /locus_tag="OO470_001282"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6250591.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1980..3206
                     /gene="mdfA"
                     /locus_tag="OO470_001283"
     CDS             1980..3206
                     /gene="mdfA"
                     /locus_tag="OO470_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6250592.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3247..3531)
                     /locus_tag="OO470_001284"
     CDS             complement(3247..3531)
                     /locus_tag="OO470_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250593.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3617..4432)
                     /gene="ybjI"
                     /locus_tag="OO470_001285"
     CDS             complement(3617..4432)
                     /gene="ybjI"
                     /locus_tag="OO470_001285"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6250594.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4432..5640)
                     /locus_tag="OO470_001286"
     CDS             complement(4432..5640)
                     /locus_tag="OO470_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250595.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5724..6260
                     /gene="rcdA"
                     /locus_tag="OO470_001287"
     CDS             5724..6260
                     /gene="rcdA"
                     /locus_tag="OO470_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6250596.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6435..8120)
                     /locus_tag="OO470_001288"
     CDS             complement(6435..8120)
                     /locus_tag="OO470_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6250597.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8390..8767
                     /locus_tag="OO470_001289"
     CDS             8390..8767
                     /locus_tag="OO470_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6250598.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8797..9054)
                     /gene="grxA"
                     /locus_tag="OO470_001290"
     CDS             complement(8797..9054)
                     /gene="grxA"
                     /locus_tag="OO470_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6250599.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9214..9501
                     /locus_tag="OO470_001291"
     CDS             9214..9501
                     /locus_tag="OO470_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6250600.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9485..10207
                     /gene="nfsA"
                     /locus_tag="OO470_001292"
                     /pseudo
     CDS             9485..10207
                     /gene="nfsA"
                     /locus_tag="OO470_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10268..11170
                     /gene="rimK"
                     /locus_tag="OO470_001293"
     CDS             10268..11170
                     /gene="rimK"
                     /locus_tag="OO470_001293"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6250601.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11258..11734
                     /locus_tag="OO470_001294"
     CDS             11258..11734
                     /locus_tag="OO470_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6250602.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12086..13198
                     /gene="potF"
                     /locus_tag="OO470_001295"
     CDS             12086..13198
                     /gene="potF"
                     /locus_tag="OO470_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6250603.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13293..14426
                     /gene="potG"
                     /locus_tag="OO470_001296"
     CDS             13293..14426
                     /gene="potG"
                     /locus_tag="OO470_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6250604.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14436..15389
                     /gene="potH"
                     /locus_tag="OO470_001297"
     CDS             14436..15389
                     /gene="potH"
                     /locus_tag="OO470_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6250605.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15386..16231
                     /gene="potI"
                     /locus_tag="OO470_001298"
     CDS             15386..16231
                     /gene="potI"
                     /locus_tag="OO470_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6250606.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16291..16779
                     /locus_tag="OO470_001299"
     CDS             16291..16779
                     /locus_tag="OO470_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6250607.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16820..17947
                     /gene="rlmC"
                     /locus_tag="OO470_001300"
     CDS             16820..17947
                     /gene="rlmC"
                     /locus_tag="OO470_001300"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6250608.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18122..18853)
                     /gene="artJ"
                     /locus_tag="OO470_001301"
     CDS             complement(18122..18853)
                     /gene="artJ"
                     /locus_tag="OO470_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6250609.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19145..19813)
                     /gene="artM"
                     /locus_tag="OO470_001302"
     CDS             complement(19145..19813)
                     /gene="artM"
                     /locus_tag="OO470_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6250610.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19813..20529)
                     /gene="artQ"
                     /locus_tag="OO470_001303"
     CDS             complement(19813..20529)
                     /gene="artQ"
                     /locus_tag="OO470_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6250611.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20536..21267)
                     /gene="artJ"
                     /locus_tag="OO470_001304"
     CDS             complement(20536..21267)
                     /gene="artJ"
                     /locus_tag="OO470_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6250612.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21285..22013)
                     /gene="artP"
                     /locus_tag="OO470_001305"
     CDS             complement(21285..22013)
                     /gene="artP"
                     /locus_tag="OO470_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6250613.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22231..22746)
                     /locus_tag="OO470_001306"
     CDS             complement(22231..22746)
                     /locus_tag="OO470_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6250614.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23395..25164
                     /locus_tag="OO470_001307"
     CDS             23395..25164
                     /locus_tag="OO470_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6250615.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLDKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25375..25698
                     /locus_tag="OO470_001308"
     CDS             25375..25698
                     /locus_tag="OO470_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6250616.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25695..26525
                     /gene="amiD"
                     /locus_tag="OO470_001309"
     CDS             25695..26525
                     /gene="amiD"
                     /locus_tag="OO470_001309"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6250617.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26522..27535)
                     /locus_tag="OO470_001310"
     CDS             complement(26522..27535)
                     /locus_tag="OO470_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6250618.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27634..29064)
                     /locus_tag="OO470_001311"
     CDS             complement(27634..29064)
                     /locus_tag="OO470_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6250619.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29075..30076)
                     /gene="ltaE"
                     /locus_tag="OO470_001312"
     CDS             complement(29075..30076)
                     /gene="ltaE"
                     /locus_tag="OO470_001312"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6250620.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30113..31831)
                     /gene="poxB"
                     /locus_tag="OO470_001313"
     CDS             complement(30113..31831)
                     /gene="poxB"
                     /locus_tag="OO470_001313"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6250621.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31964..32932)
                     /gene="hcr"
                     /locus_tag="OO470_001314"
     CDS             complement(31964..32932)
                     /gene="hcr"
                     /locus_tag="OO470_001314"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6250622.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32944..34596)
                     /gene="hcp"
                     /locus_tag="OO470_001315"
                     /gene_synonym="priS"
     CDS             complement(32944..34596)
                     /gene="hcp"
                     /locus_tag="OO470_001315"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6250623.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34740..35639)
                     /gene="lysO"
                     /locus_tag="OO470_001316"
     CDS             complement(34740..35639)
                     /gene="lysO"
                     /locus_tag="OO470_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6250624.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(35792..>36089)
                     /locus_tag="OO470_001317"
     CDS             complement(35792..>36089)
                     /locus_tag="OO470_001317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250625.1"
                     /translation="WHFICPGCFIFDITNRTGIGGVKIAEAFPEGRGSPHGCGLRARL
                     TGRYLAPDPVAEGDKLKVPRSGDFVRQSPGVQGAAATGCPLRAPSASGFIWHCT"
BASE COUNT         8982 a         8936 c         9279 g         8892 t
ORIGIN      
        1 cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg cagcttaccg
       61 caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac ggtaaatcca
      121 ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc tttggtagga
      181 tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc tcttaatctt
      241 ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca tcaacttaat
      301 acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac tcaccacaat
      361 atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt tactgtggtc
      421 gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg gcaactcttc
      481 aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat aaaaagcgat
      541 atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa tggcgttgct
      601 ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt tcagcgccag
      661 aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa tcgcttcaat
      721 aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt ctaccagtgt
      781 cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt gatcgcttaa
      841 caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca aaacgacggg
      901 cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa gcagggcggc
      961 ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct gcccgatacg
     1021 ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc cgctcaatac
     1081 tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta atgcaaaaca
     1141 gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt gccagataat
     1201 ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat ccagcggcca
     1261 gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca ttaaaagtga
     1321 gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa tcaccgtacc
     1381 gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc cgatattttc
     1441 gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa acaggctgac
     1501 cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc cccacaacca
     1561 aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa aaatcgccaa
     1621 cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata gagagagatt
     1681 caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa tacaggcaag
     1741 tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat ttccctcaag
     1801 cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt aaagaaagcg
     1861 tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta caaaattcgc
     1921 tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga agaaattgca
     1981 tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt ttccctctct
     2041 gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa cccggtatgt
     2101 tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg atgaccgcgt
     2161 atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat cgtattggtc
     2221 gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg gcaatattac
     2281 tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata agcttctgtt
     2341 tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg gtttgtatca
     2401 agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt ccgctggtgg
     2461 gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt gccgcattgg
     2521 cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg cgtataggcg
     2581 agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag aacggccgct
     2641 ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc gcccagtcgc
     2701 cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg ctgcaagtgc
     2761 ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc tcgcgccgca
     2821 ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg ttggtcgctg
     2881 ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg ttaagtattt
     2941 atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg tttgccagcg
     3001 atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg atctttaccg
     3061 tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg tttaatctct
     3121 tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta aaagataaac
     3181 agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg atcaggcatt
     3241 ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt cacctacgct
     3301 ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag gagccacata
     3361 gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg aatcaggaac
     3421 cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca tcgccacacc
     3481 agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca tttcgcctga
     3541 aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca ttgtgttgcc
     3601 gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact ttatcgatca
     3661 catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct gcgacgactg
     3721 cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc tcaatatcgt
     3781 taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat ttctgcagtt
     3841 ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg ccgttgccgg
     3901 tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt acttgtggaa
     3961 tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag ttgtcaaagt
     4021 tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg gcatcgtcat
     4081 attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact tccgggcgcg
     4141 tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca ttaaaaacat
     4201 ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt tcattcgcaa
     4261 tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc acaaagcgaa
     4321 tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta taggtttttt
     4381 gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc atcaactatt
     4441 ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga caatagccgc
     4501 gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat gttcgccgag
     4561 atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg ctaccacact
     4621 gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca gcgggaagcc
     4681 cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg cgctatcgac
     4741 aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa ccacggcaac
     4801 gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca tccccagggt
     4861 aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat caaccattaa
     4921 taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca ctacaacacc
     4981 aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat gctgggtgcc
     5041 atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat aaataggcgc
     5101 gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa aggccgtcag
     5161 tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt gcatcatcgg
     5221 caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta tcgccacttc
     5281 cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca ggggcgatgt
     5341 cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca tcgtgaccag
     5401 gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa taccgctcat
     5461 cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga cagagagaat
     5521 atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg gcaaaaagaa
     5581 gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat ttacggtcat
     5641 agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta catttgtaca
     5701 caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg cgagaaaaaa
     5761 ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt acgcaccgca
     5821 aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt tcaggaattg
     5881 atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg caatatcagg
     5941 cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc gatatgatct
     6001 acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc tacgcgctgg
     6061 ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc agtcagcaaa
     6121 cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt attgagggga
     6181 tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt ttgaggatgg
     6241 ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat caaccttgaa
     6301 ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa gcagtggcac
     6361 taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca
     6421 gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt ccctgccagc
     6481 gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg gatgttactg
     6541 cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg
     6601 ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa acagataacc
     6661 acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat cgcgcccagg
     6721 ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac catcaagccg
     6781 aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg gtggttagca
     6841 cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc catgccgaaa
     6901 ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac aaagaaggcg
     6961 caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat gcgatcggcg
     7021 atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt gttaagcacg
     7081 acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg
     7141 tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt tttaacgacc
     7201 acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc tttaccgtta
     7261 cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg
     7321 cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg tcgaatacgt
     7381 tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt gccgtcggtc
     7441 caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata aaccttacgg
     7501 ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc
     7561 tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag accgattaaa
     7621 taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg cctgctttcc
     7681 atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga cgtcatagag
     7741 cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc taaccctaag
     7801 gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt tttcccatca
     7861 cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa aataaacagc
     7921 ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc taataacagc
     7981 gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg taactttccg
     8041 agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc atttaacaat
     8101 tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct gttgaaagaa
     8161 atggtaattt acgataatgt gttttaccag aattcagggc gcagattcat tcagcgcacc
     8221 taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt atcaacggct
     8281 atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc ttactgaacg
     8341 ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag tgaaacataa
     8401 acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt gcctttttct
     8461 ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg gcttgctgtt
     8521 tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag tcctgaaacc
     8581 tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc tgtttttttc
     8641 accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt tctggtgtgc
     8701 gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc acagaaaaaa
     8761 tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat tttctttcac
     8821 ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa tctgcggcac
     8881 ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc cttccgcacg
     8941 aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct cagccagatc
     9001 ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt gcattatttc
     9061 tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg aaagtaggtt
     9121 taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag tgaaaagttt
     9181 tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa attgcctaaa
     9241 ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct gctgtcggta
     9301 agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat tctgatgatt
     9361 tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca ggtggcaaaa
     9421 cgtcttgccc cacagctgat gaaccgtcca ccgcaatatt cacgttcaga aagagaaaaa
     9481 gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc atttcactga
     9541 tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg cgacgtccag
     9601 ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat tacgtgaaga
     9661 actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt tctgagtgtt
     9721 ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg gcctggcgga
     9781 acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat taaccgcagc
     9841 ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata ttgaagcggt
     9901 gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt gccttggctg
     9961 gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg tgcatgaaaa
    10021 cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac tggcggaata
    10081 ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata tccgccgaac
    10141 aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg gttgggcgac
    10201 gcgctaaaac cgctacgtcg atgtatgata cgcgggcttt tgaccaggtc tgacagagag
    10261 gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt gtaagcggct
    10321 gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc tttcttgcta
    10381 catgaacata aatcctgcgg cgtcttctat tcactacaaa ggccgcaagt taccccattt
    10441 tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg cggcactgcg
    10501 ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg cccgggcgcg
    10561 tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc ctgtcacggg
    10621 cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg gtgcgccgct
    10681 ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg agactcgtca
    10741 ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc tggtgcagga
    10801 atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg atgaagtggt
    10861 cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc atcgtggcgg
    10921 cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg
    10981 aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt
    11041 gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg gtatcgatat
    11101 cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt gcctgaaaac
    11161 gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt tgcgtaagct
    11221 gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg tcgttcctgg
    11281 tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg aatgtgataa
    11341 ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg atgccaaaat
    11401 cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac cttcagccgt
    11461 attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg tgaaagcatt
    11521 tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt tatccgttat
    11581 gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag agcagatttc
    11641 gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg atgaagggca
    11701 aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca cgcagtcgct
    11761 gctgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct taaagattat
    11821 ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat aaaaatttgt
    11881 taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt attcttgcga
    11941 tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta caccgcgccg
    12001 ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg ttcgtttgtt
    12061 aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc tatcgggtct
    12121 ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac aaaaaacact
    12181 ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt ttgaaaaaga
    12241 aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg aaggcaaatt
    12301 aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc tagaacgcca
    12361 gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt ggaagaatct
    12421 cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat ttgctatgcc
    12481 ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag ctgtgctggg
    12541 cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc tggaaaaact
    12601 gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta ccgtgttgaa
    12661 ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac cggcaaccga
    12721 tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat acattaacga
    12781 cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct ggcaggcgtc
    12841 aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc caaaagaagg
    12901 ggcgatggcg ttctttgatg tattcgccat gcctgcggat gccaaaaaca aagacgaagc
    12961 ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt ccgaccatgt
    13021 gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag tccgtgagaa
    13081 cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga aagtgcagga
    13141 cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg gaaaataatc
    13201 cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac catcatggtg
    13261 cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct cgcccgcagg
    13321 cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc aaatcctacg
    13381 atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa atcttcgcgc
    13441 tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca ggtttcgaac
    13501 aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt ccgccttacc
    13561 tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg accgtggaac
    13621 agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt tccagtcggg
    13681 tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa ccgcatcagc
    13741 tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag cgcccgaaac
    13801 tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac cggatgcagc
    13861 ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc acccacgatc
    13921 aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg aaatttgtcc
    13981 agattggcga accggaagag atctacgagc atccgactac ccgctatagc gccgaattta
    14041 ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat ggcctggtgc
    14101 ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg gtggtcgata
    14161 acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa gagccgcccg
    14221 ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc ggcgatcttt
    14281 cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta caaaacgccc
    14341 atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc tgggaagtgg
    14401 acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa cctgctgccc
    14461 agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg aagcatgggc
    14521 gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg ctgccatttc
    14581 tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct tataccgaac
    14641 tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat tttctgcaac
    14701 tgaccgacga ttcgctctat ttcgatgctt atctccagtc gctacaggtg gctgcgattt
    14761 cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg cacagtaagc
    14821 cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc tcgtttctga
    14881 tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat aattttctgc
    14941 tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg gcagtttata
    15001 ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc gcgttgattc
    15061 gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg ctgaaaacgt
    15121 tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg atgctggtgt
    15181 ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg gacagcatca
    15241 tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg gtggcctcgg
    15301 cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc cacaaacacc
    15361 agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt cgccctggcg
    15421 gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga tgctggttat
    15481 ctattcgttt aacagctcga agctggtgac ggtgtgggcc ggctggtcaa cgcgctggta
    15541 tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc tgacaattgc
    15601 tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg tgctggtgcg
    15661 ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc cgctggtaat
    15721 gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg ctcatgccat
    15781 tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca ctttctgtac
    15841 ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct cgatagaaga
    15901 agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta cgctaccgat
    15961 gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc ttgatgatct
    16021 ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc tggtcttttc
    16081 cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga tcctcggcgc
    16141 ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa aacagcggat
    16201 acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa cctggctaca
    16261 taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg attttttaag
    16321 aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt ggcggcgctc
    16381 gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct gggcgtgcgc
    16441 ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct ggtcttttta
    16501 agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt gaaagggcgt
    16561 cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta cttgtgggcg
    16621 gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc aaaacgtgaa
    16681 atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat gctgctgttc
    16741 gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc gtcgcccctg
    16801 atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct gtcgttcctg
    16861 tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg atcttaaaaa
    16921 tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc cggaacaagg
    16981 gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac tgctcggtat
    17041 gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc cagcctcatt
    17101 tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa cgccctacaa
    17161 cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc agagtgatgg
    17221 cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac tgcgtaaggc
    17281 gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca atattcagcc
    17341 ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac aacaggcact
    17401 ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct tccagactaa
    17461 tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac agctgccggt
    17521 taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact gcgcgacgcc
    17581 tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg caaagcagtc
    17641 agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca ctcagtttgc
    17701 cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc gcggcattgg
    17761 taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct actccagctg
    17821 taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca tcgaacgggt
    17881 acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc tgctggtgaa
    17941 gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt tgttcatgcc
    18001 ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat acattgcaga
    18061 gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc atcagtctca
    18121 attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct ttaattgctg
    18181 ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca gcgataccca
    18241 ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc agttgcggat
    18301 tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca atacgaccat
    18361 ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc acttccgggt
    18421 gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg cgtttgcctt
    18481 tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct gagttttcat
    18541 agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc ataccggaga
    18601 ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac gcgtgattag
    18661 taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg atatcaaagc
    18721 cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct gaaacgccaa
    18781 aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa gcggcaagaa
    18841 ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc cgtgtctgcg
    18901 gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt aaataattta
    18961 aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat aagaatttat
    19021 gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc gtgctgatgt
    19081 ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa tgtacgcagc
    19141 tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc atcatcagcg
    19201 tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc attacatcgt
    19261 aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta atggtgtaag
    19321 ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag agcgagcgtt
    19381 taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc agggcgctac
    19441 aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat
    19501 acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct gacaacaaat
    19561 gccacagtgc cggatactcc tgcaacgtcg gaaattggcc cggcccgtaa taaatcagga
    19621 agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc accagccaca
    19681 ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga atcagtgcca
    19741 caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc agctcgggta
    19801 aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt
    19861 gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca caatgtacca
    19921 ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta aatcattcac
    19981 actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt taccgaggcc
    20041 aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag ccgatttcga
    20101 cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca acgcgccgcg
    20161 cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac cacaaaggaa
    20221 cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga acccaagatt
    20281 gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat agataaacag
    20341 cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac ctgcccacgc
    20401 gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg ccagcccgac
    20461 aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa
    20521 ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt aagtgccatc
    20581 tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt tgccctgacg
    20641 taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat cgcccactgc
    20701 cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac caaatacgct
    20761 gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat acggaacggt
    20821 ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt tctgaacgcc
    20881 gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac ccacaaacag
    20941 ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct ccggagtgat
    21001 atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga tcaggctgtc
    21061 aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg cctgtgccag
    21121 gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg gaggataggg
    21181 ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa aacctgcaat
    21241 taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg agagagatag
    21301 tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg ttctacgata
    21361 tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc aacttcgtgg
    21421 gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct gacgatttgt
    21481 gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac ctgcggttcc
    21541 atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag atgcagcggg
    21601 taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc acgggccagc
    21661 gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt ttgctgcacg
    21721 gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg acgcaaatcg
    21781 cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc
    21841 gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct tttacccgcg
    21901 ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac
    21961 agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact cattgacact
    22021 cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac agaataatta
    22081 tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa catagcacaa
    22141 aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa atgccggata
    22201 atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact gacggagcgt
    22261 acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga cccaacactg
    22321 gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt tgagatccac
    22381 ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg caacgtgggc
    22441 gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt cacgatgact
    22501 attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat aggggcgcag
    22561 ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt gagccacact
    22621 gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat aagctggcgt
    22681 gacggtggta caggcactca gcagcagtgc acatggaata agtagagata gtgaacggta
    22741 gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca aacgctatca
    22801 gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc tgattgggtt
    22861 tgagtccatt gctggctacc cactcaaggt tgctcgtagc tgacttactt aaattaccca
    22921 aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg aaatgatcat
    22981 ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga ggttaagagc
    23041 atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg ttattgtcta
    23101 atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga ttgccaaaat
    23161 tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact ctcgataata
    23221 ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt gaagaataaa
    23281 ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt gcttttataa
    23341 ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt taatatgata
    23401 aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca aagtgataat
    23461 ttcctggatt cactgaggga atttttcgcg atattaaatt ccagtcgtaa gggtgagttg
    23521 tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga cagtgatctc
    23581 ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca atttaataca
    23641 ttttttagca atactttcga gattgatgtt aaaaaatatc tggataaatc agaagataac
    23701 gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa tgtatcgaat
    23761 ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa attacaagaa
    23821 caattagata agtattttac gattgaatcg cacccattat tatacaggta taccattggt
    23881 agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt taacaaagaa
    23941 caatatatat cctttataaa ggacgcattt attcattttt atgattatag caggcgatat
    24001 gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt gatgagcact
    24061 ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac agaagaagag
    24121 tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc tgatgataat
    24181 tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc ctttcaagat
    24241 accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat ttgtagcgta
    24301 agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa tgaagaagtt
    24361 ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag taatttagtc
    24421 aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt taatgacgtg
    24481 gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt gaatgaaagc
    24541 atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat aacaaatagc
    24601 aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat taataacgta
    24661 aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat gataagagtt
    24721 aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat aaaaccctgg
    24781 ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg tttactatct
    24841 ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa cgtgaaggtt
    24901 gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg tattaaacta
    24961 ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt cgcttatatt
    25021 aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga ccaaaaagca
    25081 atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca tttacaatat
    25141 aagttgcctc tttccgctca ttaatgttgt tggtgcaaca taataagaca ggtaagcggt
    25201 accctataat gaaaccaata tcgggttatg caacggataa ttgtaaagac ttaagtgggt
    25261 gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc aacatggcat
    25321 atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc gatcatgcaa
    25381 ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg tgtggtgacc
    25441 ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat ccgcgatatc
    25501 gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga gatcgccttt
    25561 gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg tattgatatc
    25621 gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag cggtacggcg
    25681 gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg ttactggcag
    25741 ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat acccgacacc
    25801 aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc gcagatgatt
    25861 ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat ctgatccctg
    25921 cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg gaacaagaac
    25981 tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac gacacctcta
    26041 ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa tattttgccc
    26101 cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt attgcccgtt
    26161 atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag cgcaaagacg
    26221 atccggggcc attatttcct tggcagcaac tggcgcagca ggggattggt gcctggccgg
    26281 atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg gtagataccg
    26341 cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat atgacaccgc
    26401 gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg ttatataacg
    26461 gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa tacgggcagg
    26521 attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg atgccttcat
    26581 ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata tccagcgtaa
    26641 agtcaaaatt caacttggag acgccgtagt gggtcagcgg cggctctttt gctgacttgc
    26701 ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg acggaacgaa
    26761 tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc agtgtgcgat
    26821 gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag gcttcctggc
    26881 ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc agcgcactgc
    26941 cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga cggggaataa
    27001 agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag cgcgtttgtg
    27061 gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg cgggcaaact
    27121 cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg tgataatcga
    27181 agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg acagcccatt
    27241 cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt gtcccccagg
    27301 gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc actttagctt
    27361 gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt ttttccagca
    27421 atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc tggcataaaa
    27481 actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc ttcatatcaa
    27541 tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca tgatgcaatg
    27601 ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt ctgccagtct
    27661 ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca tcaataacca
    27721 gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga tagtacgata
    27781 gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt tcatgccaaa
    27841 taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat ccaccgcatc
    27901 gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac cgatcgcgcg
    27961 gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct ctttaccgcc
    28021 gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa cggtaaaccc
    28081 cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct gagcgtcgta
    28141 gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac gtaccgcgtc
    28201 atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat ctgccagcag
    28261 atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg aagtaatcac
    28321 attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca accagcggcg
    28381 cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca cctctggtcc
    28441 ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca ccagatagtg
    28501 cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa
    28561 cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac ctgcgccaac
    28621 gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa tgtccgccgt
    28681 agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca acgaactgag
    28741 aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga gagccagctg
    28801 gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa aatagacggt
    28861 atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga ctttatggca
    28921 actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg ccgctaggat
    28981 ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc cactggcacc
    29041 gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat gcgcgccagt
    29101 gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc aggcggacaa
    29161 tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac gcggcagcat
    29221 tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca tccgcgcctg
    29281 cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga
    29341 cgttattttt cagggcatac atcccggcgg cagccagaat cccggactga cgcatcccgc
    29401 caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta ccgacgagta
    29461 atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa tcacaatatt
    29521 gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg cgcgcaccgt
    29581 caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc aggtattcac
    29641 gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg cgggcgaaat
    29701 ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag
    29761 ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg gcttcaaaca
    29821 gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag tgactgagca
    29881 gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa
    29941 gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa acgtcgtccc
    30001 caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta acggtatcac
    30061 tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct ttttacctta
    30121 gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata attgcgcgca
    30181 acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga atggctaatt
    30241 cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag gcgcgttgca
    30301 gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg cacgcttcgg
    30361 caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa cctccggcct
    30421 tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt ttcaccggca
    30481 gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc
    30541 acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc tgcggcatgg
    30601 cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg ttcattttca
    30661 gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg gcgtcatcgg
    30721 cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc tcgctcggtt
    30781 tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa
    30841 acttgcgatc ggttttttct tccaccagtg gcagcaatgc gcgcagggtt gatttgatat
    30901 cgccgaccag cgccatatct accttgctat gcgcgccgat gctggctgga ttaatatcaa
    30961 tctgaataat tttggcatcg gtcgggtaga acgcacggta ggggaactga gtaccgagta
    31021 gcactaacgt gtcggcgttc atcatggtat ggaaacctga cgagaatccg attaacccgg
    31081 tcattccaac atcatacgga ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa
    31141 caataggtgc tttaattttc ccggcaaact caactaactc tttatgcgcc cccgcgcagc
    31201 cgctgccaca catcagggcg atattgctgg aataacgcag cagttgcgcc agtttgcgta
    31261 actcttcttc ttccggcatc acgactggct gtggcgcatg ataccagtgc gtggttgccc
    31321 cttctggcgc aggttttaac gccacgtcgc ccggtaatac aacaacggaa acgccacggt
    31381 taagcaccgc tttgcgcata gcaattgcca gcacttgtgg gatctgctcc gggctggaaa
    31441 ccagctcgca atagtgacta cattcgcgga atagctcttg tgggtgggtt tcctggaaat
    31501 agccgctgcc aatttcgctg gagggaatat gagcggcaat cgccagtacc ggaacgttat
    31561 tgcggtggca atcgaatagg ccgttgatca agtgcaggtt gcctgggccg cacgatccgg
    31621 cacagaccgc cagttctcct gaaagttgtg cttcagcgcc agcggcaaac gccgccactt
    31681 cttcatggcg ggtggacatc cactcgatgg tgcccatgcg attaagactg tcgctaagac
    31741 cgttcagaga gtcgcccgtg actccccaga tgcgtttcac gcctgccgat tcgagtgttt
    31801 tggcgatata agctgcaacc gtttgtttca tggttctcca tctcctgaat gtgataacgg
    31861 taacaagttt agttcatctg acggaggggg aagggatggg agagaaagga ggcactaacg
    31921 gttaaatagc ccgatgaaag gaatatcatc gggcataagg cgattatgcg agaaccaaat
    31981 ccccctgcgg atggcaggag caggccagta cgtaaccttc agcgatttcg gcgtcggtca
    32041 gcgtcattgt actgctcacc gtatattcac cggaaaccac ttttgtctta cagcagccgc
    32101 aaacacccgc acggcaggca gcgacaaccg gaacgttatt gctttccagc gcctccagta
    32161 gcgtggtgcc aaccggggcg taaaattctc gtgccggttg cagtttggtg aatttcagac
    32221 cgctggtcgc cgcttctgct actggggtga agaatttctc tttaaagaaa cgcgtcacgc
    32281 cgagcgcttt cacttcctgc tctacccaat ccatatacgg agccgggccg caggtcatca
    32341 cggtacgtga agctaagtca ggtacatctg ccagcagttc gcgagtgaga cgaccagcga
    32401 taaagccttc ggtaacgtta ttttctgcca ccagcgttac cggatagtta cgccactcat
    32461 cggcgaaaat aacatcctgc ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg
    32521 gacggttctt cgcaagccag cgacgcatcg acataatcgg cgtgacgccg cagcctgccg
    32581 ccagcaacag gaatttatct tctgctttat cgtcgcaggt aaattccccc atcgcgtccg
    32641 aaagccagag ataatcaccg cgttttacat cgcgcgtcag ccactgggag ccgacaccgt
    32701 catcaatccg ccgcacggtc agggtgatat attcactcac gcctggcgtg gaggaaatgg
    32761 tgtaagcacg cagcgtttcc gctgagtttc gcacgctgac cagtgcatat tgcccggcgc
    32821 gatatgggta gtgatcgtgg caaatcaggg aaatcgtcca cacatccggc gtttcttgcg
    32881 taatgtgatg aacctgtatc cgccacgggc attgattcgt tggcatcgtc atcgacaaac
    32941 tccttacgcg ttcaacagtt gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc
    33001 gaatttctcg ttcagcaccg ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc
    33061 ggtgacgata tttttcacgc ccagagaaag cagcgtcagc agaatgacaa tcgctttctg
    33121 ttcaaaccag gagagcacca gcgacagcgg cagatcgttc acaccacagc ccagtttctc
    33181 tgccagagtg acagccagaa taattgctga gtaagcatcg ttacattgac ctgcatctac
    33241 cagacgcggc agaccttcga tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca
    33301 ggcgagggtc aggatcaggc agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg
    33361 gcgctcgccg cgtgcgccgt cacagccacc aagcaggaag atatgacgca gtttttcacg
    33421 gctaaccaga tcaatcagcg tatcggctgc accaagcagc gtctggcgac caaagcccac
    33481 ggtgatcagg tgaggaattt cgctgtacgg gaagcccgcc atttgttgcg cctgggcgat
    33541 gaccgcagag aaatcttcgc catccagatg acgcacgcca ggccagccaa cgatgctgcg
    33601 ggtccagata cgatcgtcat aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat
    33661 cacgatgggg ccagggaaac gagcgaactc cacttgctga ttctgccagc cgctgccgta
    33721 gttaccgacc agatgcttga atttacgcag ctccgggtag ccatgcgcag gcagcatttc
    33781 gccgtgggtg tagacattaa cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc
    33841 tttgagatcg tgaccggaaa tcagaatgca tttacccgcc gtcgctttga cgttgacctg
    33901 ggttggcgtc gggtgaccgt atttaccggt ttcgcctgca tccagaatgc tcatcacttt
    33961 gaagttcatc tggccgattt ccattgaaca ctcaagaagc gcgttcatat cggcaggcca
    34021 ggtccccagc cacgccatga ttttatggta ctgggcataa atatcgttgt cgtattgacc
    34081 gagaacatgc gcgtgttcca tataggccgc cgcacctttc aggccataca ggcacagcag
    34141 acgcaggccg agaatgtttt cgccaatcgc cgctttatct ttgttagggg taaattctgc
    34201 tgcctgacgt tgcagctcgc cgagatcatc gctcaccagt tgcaggtcag ccatcgggtt
    34261 atcgacgcgc gcgttggcat ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc
    34321 aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt tggtcagggt
    34381 tgagaaaaag gcacgtggcg cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg
    34441 cgcttttacc gcccaggcag aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc
    34501 agaagtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt tttctgccgg
    34561 agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta aagttatatt
    34621 taatatacat gtttaagggt aagacgctta acgcggggat aaaagggatt tttcatgcaa
    34681 ctttaaggga gattgattta gcgcaatttt ggcggcaggg aactaccgcc agagaggtat
    34741 tacgcagaga aaaaggcgat gaggatcggc actaacaggc taagaataaa accgtgaaca
    34801 attgccgccg ggaccatatc cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc
    34861 attgatgtgg caccgcataa gcccagtgca gtagagcggc tgcggcgaat cagcccaggg
    34921 atcaacataa tagcaatcag ttcacgggcc agatcattaa aaaacgccgc gctcccgatt
    34981 accggaccaa aagattcggt caataaaata ccggaaagag aataccagcc gaaaccggag
    35041 gccattgcca gcgcggtatt gatggggaga tcaagaataa aggcgttaat taaaccacca
    35101 attaatgaac tgacaaccac caccacggcg acaatcattc cccggcgatt aaggacaatc
    35161 tgctttaagg tcatgccatt attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt
    35221 aacgtgtatt cactggcttc ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg
    35281 gcaaaaccaa tcactactac gccgcacagt tttagcgact ccagcgccat cgcaatacgc
    35341 gacgggagtt tttcttgctg atggtggttg cgccacggca ggcctcgctc cagccacatc
    35401 agggcggcaa tattacacag taaaataacg gtaatactga cggcagaata atgcagaatc
    35461 gccaacaggt tactggcgag gttatcgaga aacgccagac tgatacccat aaaaaagaga
    35521 ataaggtaaa ccatccagct taatagctga ttaatagctt ttaacgcagc ttgttggcga
    35581 agcggaatga ggtaacccac aatcagggga accagaatga ttaacagccc agaaaacatg
    35641 aaaatccagt ccttgcaaag aagaagtcga aatgcgcgat gacacactac tgaaagcgga
    35701 agggcgagta aagttgcaat taaaaggaaa tgttatgcat cagaggggga tttttaatcc
    35761 tatctgagtg tttagcattg ttgcgctcct ttcatgtgca atgccatata aagccactgg
    35821 cggagggagc gcgcaggggg cagccagtcg ccgccgcccc ctgcaccccc gggctctggc
    35881 gaacaaaatc gccgctgcgc ggtaccttca gcttatcccc ttcggcaacc gggtcgggcg
    35941 cgaggtaacg tcctgtaaga cgcgccctca gcccacatcc atgtgggctg ccccggcctt
    36001 cggggaacgc ttcagcgatt ttgacgccac caatacccgt gcggtttgta atgtcgaaga
    36061 taaaacaacc agggcagata aaatgccaa
//
LOCUS       DAJTUN010000028        34894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTUN010000028 DAJTUN010000000
VERSION     DAJTUN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.028"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(18..93)
                     /locus_tag="OO470_001318"
     tRNA            complement(18..93)
                     /locus_tag="OO470_001318"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="OO470_001319"
     tRNA            complement(152..228)
                     /locus_tag="OO470_001319"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="OO470_001320"
     CDS             complement(331..1716)
                     /locus_tag="OO470_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6250626.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO470_001321"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="OO470_001321"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6250627.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO470_001322"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="OO470_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6250628.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO470_001323"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="OO470_001323"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6250629.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO470_001324"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="OO470_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6250630.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO470_001325"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="OO470_001325"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6250631.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO470_001326"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="OO470_001326"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6250632.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO470_001327"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="OO470_001327"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6250633.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO470_001328"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="OO470_001328"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6250634.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO470_001329"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="OO470_001329"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6250635.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO470_001330"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="OO470_001330"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6250636.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO470_001331"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="OO470_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6250637.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO470_001332"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="OO470_001332"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6250638.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO470_001333"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="OO470_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6250639.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO470_001334"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="OO470_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6250640.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO470_001335"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="OO470_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6250641.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="OO470_001336"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="OO470_001336"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6250642.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="OO470_001337"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="OO470_001337"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6250643.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO470_001338"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="OO470_001338"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6250644.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="OO470_001339"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="OO470_001339"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6250645.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO470_001340"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="OO470_001340"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6250646.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="OO470_001341"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="OO470_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6250647.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO470_001342"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="OO470_001342"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6250648.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO470_001343"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="OO470_001343"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6250649.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO470_001344"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="OO470_001344"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6250650.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO470_001345"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="OO470_001345"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6250651.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /locus_tag="OO470_001346"
     CDS             complement(29521..31167)
                     /locus_tag="OO470_001346"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6250652.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /locus_tag="OO470_001347"
     CDS             complement(31170..31220)
                     /locus_tag="OO470_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250653.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO470_001348"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="OO470_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6250654.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33276
                     /locus_tag="OO470_001349"
     CDS             31756..33276
                     /locus_tag="OO470_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR6250655.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33301..33639)
                     /gene="maoP"
                     /locus_tag="OO470_001350"
     CDS             complement(33301..33639)
                     /gene="maoP"
                     /locus_tag="OO470_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6250656.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33758..34597
                     /gene="hdfR"
                     /locus_tag="OO470_001351"
     CDS             33758..34597
                     /gene="hdfR"
                     /locus_tag="OO470_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6250657.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34693..34768)
                     /locus_tag="OO470_001352"
     tRNA            complement(34693..34768)
                     /locus_tag="OO470_001352"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34733..34735),aa:Trp,seq:cca)
     gene            complement(34777..34853)
                     /locus_tag="OO470_001353"
     tRNA            complement(34777..34853)
                     /locus_tag="OO470_001353"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34817..34819),aa:Asp,seq:gtc)
BASE COUNT         8505 a         9556 c         8811 g         8022 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccggtactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 atattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg tacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacgggc gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtggaatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc gatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatta ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca accggctctc ggggcccttt
    32881 ctcgaccgct tcgatctctc actggagatc ccattaccac cccccggcat tttgagtaaa
    32941 acggtagtgc cgggagaaag cagcgccacc gttaaacaac gcgtaatggc cgccagagag
    33001 cgccaattta agcggcagaa taaactgaat gcctggctgg atagtccgga aatacgccaa
    33061 ttctgcaaac ttgagagcga agatgcgatg tggttggaag aaacgctgat ccatctgggg
    33121 ttatcgattc gtgcctggca gcggttactg aaagttgcac gaaccattgc tgatattgat
    33181 cagtctgaca ttatcacacg tcagcatttg caggaggcag ttagctatcg tgcgattgac
    33241 cgtttgctca tccatctgca aaaactactg acataaaaaa agggcatttc gcccttttta
    33301 ttaatcgtca gaatcggtgt agtcttcagc accttcaacc tgcggtttac cgccggaaag
    33361 ggtgtgaaaa cgttttggac gcttaatacg cgtcatatac ttggaccaca cgcgttctgc
    33421 ttctgtcact ggctcacgtt cgccacggca tactgctacg aagagtttct cttcctcggt
    33481 aaccggctcg cgtttgccaa gatccaactc attgaaggca taaccatgac gctcaagcag
    33541 ttgtgcctct ttgatggtga aatcaccatg acgagagaat ccacgtggat aatgtttatt
    33601 gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt
    33661 gaccgagcta gttatggcgc ggagtattag ttacgcttga cagagtgtaa aacaaaacat
    33721 ttaaatcata acgacaaata attttgcgga gagcactgtg gatacggaat tgttaaaaac
    33781 tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg gctgaatcgc tctatctgac
    33841 ccagtcagca gtgagctttc gaatcagaca actggaaaat caactgggtg tgaacctttt
    33901 cacccgccac agaaacaata tccgtttaac cgctgccggt gaaaaactac tgccttatgc
    33961 agaaacgctc atgagcacct ggcaggccgc ccgcaaggag gtggcgcata cctcacgaca
    34021 taacgagttt tctatcggtg ccagcgcctc gttgtgggaa tgtatgctta atcagtggtt
    34081 gggacgcttg tatcaaaatc aggatgccca tacaggctta cagttcgaag cgcgaattgc
    34141 ccaacggcag tctctggtaa aacagctgca tgaacgccag cttgatcttc ttattaccac
    34201 tgaagcgccc aaaatggacg aatttagtag tcagttgctg gggtatttca ctttagcgct
    34261 ttataccagt gccccttcta aactaaaggg agatcttaac tatctgcgac tggagtgggg
    34321 gccagatttt caacagcatg aggcaggttt gatcggtgct gacgaagttc ccattctgac
    34381 aaccagttct gctgaactgg cacagcaaca gattgcgatg cttaatggtt gcacctggct
    34441 acccgtcagc tgggcgcgta aaaaaggcgg cctgcatacc gttgtcgata gcacaacact
    34501 ttcacggccg ctttatgcca tatggctgca aaatagcgat aaaaatgcgt tgattcgcga
    34561 tcttttgaaa attaacgtgc tggatgaagt gtattaatct gaatggctgg caaggatgcc
    34621 ggtagaagga tttacttcgg agaggattat ttcagataaa aaaaatcctt agctttcgct
    34681 aaggatgatt tctggcaggg gcggagagac tcgaactccc aacacccggt tttggagacc
    34741 ggtgctctac caattgaact acgcccctaa ttagggtggc ggaacggacg ggactcgaac
    34801 ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat
    34861 tcttttacaa ccaccggttt tatgaccggc ttac
//
LOCUS       DAJTUN010000029        34693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTUN010000029 DAJTUN010000000
VERSION     DAJTUN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.029"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..2105
                     /locus_tag="OO470_001354"
     CDS             <1..2105
                     /locus_tag="OO470_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6250658.1"
                     /translation="WNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLF
                     NAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQL
                     LGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFL
                     QAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVG
                     NLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCY
                     ESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQL
                     SASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVP
                     TDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNV
                     YATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQ
                     DEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIING
                     AGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFT
                     LEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQL
                     KQPKLRGNVQTAQREVAP"
     gene            2102..3190
                     /locus_tag="OO470_001355"
     CDS             2102..3190
                     /locus_tag="OO470_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6250659.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3278..4576)
                     /gene="exuT"
                     /locus_tag="OO470_001356"
     CDS             complement(3278..4576)
                     /gene="exuT"
                     /locus_tag="OO470_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6250660.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5060..6472
                     /gene="uxaC"
                     /locus_tag="OO470_001357"
     CDS             5060..6472
                     /gene="uxaC"
                     /locus_tag="OO470_001357"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6250661.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6487..7974
                     /gene="uxaA"
                     /locus_tag="OO470_001358"
     CDS             6487..7974
                     /gene="uxaA"
                     /locus_tag="OO470_001358"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6250662.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8057..8608
                     /locus_tag="OO470_001359"
     CDS             8057..8608
                     /locus_tag="OO470_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6250663.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8613..9857)
                     /gene="sstT"
                     /locus_tag="OO470_001360"
     CDS             complement(8613..9857)
                     /gene="sstT"
                     /locus_tag="OO470_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6250664.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10453..11418)
                     /gene="alx"
                     /locus_tag="OO470_001361"
     CDS             complement(10453..11418)
                     /gene="alx"
                     /locus_tag="OO470_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6250665.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11689..12687)
                     /locus_tag="OO470_001362"
     CDS             complement(11689..12687)
                     /locus_tag="OO470_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6250666.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12766..13458)
                     /locus_tag="OO470_001363"
     CDS             complement(12766..13458)
                     /locus_tag="OO470_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6250667.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13531..14034)
                     /locus_tag="OO470_001364"
     CDS             complement(13531..14034)
                     /locus_tag="OO470_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6250668.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14119..15255
                     /gene="rlmG"
                     /locus_tag="OO470_001365"
     CDS             14119..15255
                     /gene="rlmG"
                     /locus_tag="OO470_001365"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6250669.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15540..15854
                     /gene="higB"
                     /locus_tag="OO470_001366"
     CDS             15540..15854
                     /gene="higB"
                     /locus_tag="OO470_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6250670.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15851..16267
                     /gene="higA"
                     /locus_tag="OO470_001367"
     CDS             15851..16267
                     /gene="higA"
                     /locus_tag="OO470_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6250671.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16312..18330)
                     /gene="fadH"
                     /locus_tag="OO470_001368"
     CDS             complement(16312..18330)
                     /gene="fadH"
                     /locus_tag="OO470_001368"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6250672.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18556..20907)
                     /gene="ygjK"
                     /locus_tag="OO470_001369"
     CDS             complement(18556..20907)
                     /gene="ygjK"
                     /locus_tag="OO470_001369"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6250673.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20924..21343)
                     /locus_tag="OO470_001370"
     CDS             complement(20924..21343)
                     /locus_tag="OO470_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250674.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21321..21674)
                     /locus_tag="OO470_001371"
     CDS             complement(21321..21674)
                     /locus_tag="OO470_001371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250675.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21675..21995)
                     /locus_tag="OO470_001372"
     CDS             complement(21675..21995)
                     /locus_tag="OO470_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250676.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22129..23562)
                     /locus_tag="OO470_001373"
     CDS             complement(22129..23562)
                     /locus_tag="OO470_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6250677.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23625..24074)
                     /locus_tag="OO470_001374"
     CDS             complement(23625..24074)
                     /locus_tag="OO470_001374"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6250678.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24071..27148)
                     /gene="ebgA"
                     /locus_tag="OO470_001375"
     CDS             complement(24071..27148)
                     /gene="ebgA"
                     /locus_tag="OO470_001375"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6250679.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27331..28314)
                     /gene="ebgR"
                     /locus_tag="OO470_001376"
     CDS             complement(27331..28314)
                     /gene="ebgR"
                     /locus_tag="OO470_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6250680.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28532..28863
                     /locus_tag="OO470_001377"
                     /pseudo
     CDS             28532..28863
                     /locus_tag="OO470_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28905..30284)
                     /gene="ygjG"
                     /locus_tag="OO470_001378"
     CDS             complement(28905..30284)
                     /gene="ygjG"
                     /locus_tag="OO470_001378"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6250681.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30702..32222
                     /gene="aer"
                     /locus_tag="OO470_001379"
                     /pseudo
     CDS             30702..32222
                     /gene="aer"
                     /locus_tag="OO470_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32376..32999)
                     /gene="nfeR"
                     /locus_tag="OO470_001380"
                     /gene_synonym="yqjI"
     CDS             complement(32376..32999)
                     /gene="nfeR"
                     /locus_tag="OO470_001380"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6250682.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33276..34021
                     /gene="nfeF"
                     /locus_tag="OO470_001381"
                     /pseudo
     CDS             33276..34021
                     /gene="nfeF"
                     /locus_tag="OO470_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34075..34150)
                     /locus_tag="OO470_001382"
     tRNA            complement(34075..34150)
                     /locus_tag="OO470_001382"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34115..34117),aa:Ile,seq:cat)
     gene            34275..34693
                     /gene="mug"
                     /locus_tag="OO470_001383"
                     /pseudo
     CDS             34275..34693
                     /gene="mug"
                     /locus_tag="OO470_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8465 a         8927 c         9114 g         8187 t
ORIGIN      
        1 actggaattt caacggttat gatgcagatg atgtcagtga cagtaatact gacgtcagcg
       61 atctctatta tcgttatgat tttttacgtc gttattatgt gcaggcgggt cgcatggaca
      121 accgcacact atttaatgca caaggcggga actttacctt taactttctg ccactcggtg
      181 caatcgatgg gatgcgtatc gggtcgacac tgagctattt aaaccaggcg caaagccagc
      241 aaggaacccc agtaatggtt ctgctttcgc gcaattctcg tgttgacgct tatcgtaatg
      301 agcaactttt gggatcgttt tatctcaata gtggttcgca atttattgat accagttcct
      361 ttccgccagg cagctacagc gtggcattaa aagtctacga aaataaccaa ctcacccgca
      421 ccgagcttgt gccgtttacc aaaaccggcg gtctgactga cggaaatgcg caatggttct
      481 tacaggcagg taaaactaca tcacaggttt ctgatgatga aagttcagct tatcagctag
      541 gagtacgcct gccattacat ccgcaatatg agctctacgc agggctggcg aatgccgatg
      601 atgtgagtgc tttcgagtta ggtaataact ggacggcaga tttaggcagg gtggggaatc
      661 ttgcaatcag cgccagcgtg ttccgtaacg atgacggcgg caaaggtgat atgcaacagg
      721 ccaactggag taatccggga tggccgacgt tgggctttta tcggaccaac tctgacggcg
      781 atgcttgtac aaccgacagc agagagagct ataacgcctt aagctgttat gaaagtattt
      841 ccgcgacagt ttcactgaat tttgtcggct ggaatatgat gctgggttat acctgcacac
      901 aaaataacac tgatgatagt ttgcgttggg ataaacagca gagctttgaa aataactatc
      961 ttcgccagac aacagcgcaa agtatctctg aaactgtaca acttagcgct tcccgcgcta
     1021 ttgtgatgcg tgactggatt ttgagtacgt cagtcggtgt tttccatcgt aatgacaacg
     1081 gtggcgataa cgacgacaac ggcttgtact tatcgttttc gttatctgac acgccaacga
     1141 tggatagcaa taacaacagc cattcaacca atgtgcctac ggattatcgt tatagcgaac
     1201 aggatggcga tcaaacgtca tggcagttat cgcatacctt ttataacgat tcattcagcc
     1261 ataaagaact tggcgtaacc gtcggaggcc tgaacaccga taccataaac agcgcggtta
     1321 acgggcgttg ggatgggcaa tacggaaatg tctacgctac cgtatctgat agttatgacc
     1381 gtaagaatca tgatcatctt tcggccttta cgggcactta tagctctacg ctggctgtca
     1441 gtcgctatgg cgttaatttg ggagccagtg gtacagacga tttgctgggt gcggtactgg
     1501 tggatgtgaa aggcttctct gaacaggatg aagagagtca ggatctgcaa ctcgaagcgc
     1561 gggtggcagg cagccgaact ttgcagcttg gtcaaagtga cagtgtgttg ttcccttatc
     1621 ctggatttca gtctggtttt gttgaggtta acgacagtag ccagggcaat cagcagggga
     1681 caacaaacat cattaacggt gcagggaatc gtgaattaat gttgttgcct ggcaagctgc
     1741 gctatcgcga agtgtctgcc agctttaact acaactatat cggtcgttta ttactgccgg
     1801 cagcggtgaa aaaattcccg attgtggggc tgaacagcgc catgttactg gtagctgaag
     1861 atggcggatt tacacttgaa attaacggca gcgaaaaaga gctctatctg ctttccgggc
     1921 agcaattcct taagtgtcca ctgagtgttg taaagaaacg cgccagcatt cgttacagcg
     1981 gagatgttac ttgtagtgtg gtgacttatt cacaattacc ggagtcgatt caggttcagg
     2041 cacagttgaa acagcctaaa ttacgtggaa acgttcagac ggcgcaaagg gaggttgcac
     2101 catgagaaat cgattgattg cggcgatatt gggcttgttt ggcacgctca ctggcgttca
     2161 ggcagctcct gacgtgacca gtgaaattac gtatgatttg gcatctggta gagcggatta
     2221 ttacttctgg aaggatgaag cgtcggcagg aaataatgga tatatgtggt atgaatgttc
     2281 gtatcctgac ctccaacaaa cctgtacagc taatggaaat atatcgacag tacaaatcta
     2341 tttaactgaa caacgcagtg ggatgcgttg gccggtaaaa ctcaaaggat ttaaaacagc
     2401 cattgtaagt agcgatgaag cgccgctagg atgcaagggg ggcaaagggc ttcagacgaa
     2461 tcttaaggat tctaatagat cttcatgtac agaagatggt caacattatt atatatacga
     2521 tacaaagttt cttacgctct accttgagca gacagagatg aagaatttgc cgattggtgg
     2581 cgtctggaag gggaaagtta aattacattc gaacagcccg gcccaggact atttcgcaaa
     2641 tattaccctg aatacgctcg accccaacca tattgacgtg ttcttcccgg agttcgccca
     2701 cgccacgcca agggtgcagt tagacttgca tccaacagga agcgttaacg gcagcaacta
     2761 cgcgcaagat ctgaccatgt tggacatgtg cctgtacgat ggttttaacg gtaatgccat
     2821 cagttatgaa atcatgctca aagatgaagg gcgaccagct gcagggcgca gagacggtta
     2881 cttctctatc tatcgtcagg gagggaccac caccgacgag ggagaacgca ttgattaccg
     2941 ggtcaaaatg tacaacccgg aaaccggtgg gcaaattgat gtgcgcaata atgaaaatat
     3001 ggtctggaac agcattaacc tgaaacgtgt gcgtccggtg gtactgcccg gtattcgcta
     3061 tgccgtgatg tgtgtgccga cacccttgac tctggcagtc gataaattca gcgtgatgga
     3121 taaacaggcc gggtactaca tgggcaaatt gtcagtaatc tttacgcctt ccttgccaac
     3181 catcaattaa ccctgcaaac cggacaaacg cttttttcta tataaacgac gaagccgcct
     3241 gataaggcgg cttcgtcgtt ataacttacg ttaaacgtta atgttgcggt gcgggatcgt
     3301 tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg caatacggtc cagataacca
     3361 gcgcacccag caggtcaaat actgccagaa ctgcgaacag cgggctgaag ccgatggtgt
     3421 cagccagtgc accgacaacc agcgcaaaca gcgtacttgc cagccatgcg gacatcccgg
     3481 ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac gtcagaagag agcgtaatca
     3541 gcgcgccaga cagtgcctgg tgggcaaaac caccgataca cagcagcata attgcgacat
     3601 acgggttggt gaacagaccg atcatacccg ggccaatcat cagcacagca cccagcgtta
     3661 cgaccatctt acgggaaacg atcaggttca caccaaacca acgctggaac agcggcggca
     3721 ggtaaccacc gaggatacaa ccgaggtcag caaacagcat cggcatccag gcgaacatcg
     3781 cgatctcttt caggttaaag ccgtaaactt taaacatgaa cagcgggatc cacgcgttaa
     3841 aagtacccca ggccggttct gcaaggaaac gcggcagtgc gataccccag aactgacggt
     3901 tacgcaggat ctgaccaacg gacattttct tcgccgtgct cacctggtgc tgggcttcct
     3961 gaccattaat aatatagtcg cgttcttcat cggtcagatg cttctggtcg cgcggatgtt
     4021 tatagaaaat cagccatgcc atcgcccaga taaagctcaa tgcaccggag atgataaatg
     4081 ccatctgcca gctgtgcatc acgattgccc ataccaccag cggcggcgca atcatcgcac
     4141 caatcgaaga acctacgtta aagtagccta ctgcgatgga acgctctttc gccgggaacc
     4201 attcggagct ggctttcaga cccgccggga tcatcgctgc ttccgcggca ccgaccgcac
     4261 cacgagcaac agctaggcca ccccagctac ctgccagcgc ggttgcacca cagaacacgg
     4321 cccacagcac agcaaacatt gcataaccga ttttcgtacc cagcacatcc agtacataac
     4381 ctgctaccgg ttgcatgacc gtataagcag cagaataggc tgcgatgata taggaatact
     4441 gttgggtgga gatgtttaac tcttccatca gagttggcgc agctgccgcc acagtgttac
     4501 gcgtcaggta accaagcacg gtgccgagcg tcaccagtgc gatcatatac caacgtaacc
     4561 ctttaatttt acgcatgtaa aacctcatcg ttttgttata tccgcaggaa gcgcggccat
     4621 catcccgcca gaggctgaag atgttagtta cgaccgggta accgaaaaaa aaaccggcac
     4681 gccccgaacg ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat
     4741 tcatctaaca aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc
     4801 agaaagataa cttgtcatac aaccttaaaa ggtgagagcc atcacaaatg tgcgaatatt
     4861 tgtagggaca ttacctgacg acagcgaggc cagtactggc gcggcctgca gcgagattta
     4921 ccactttgag agtaattttt ttaactacgt ttattgatct aactcaagaa aatatcttcg
     4981 gactctggaa attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact
     5041 cgttgagagg aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga
     5101 atttgcccgc cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg
     5161 ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg
     5221 gttgaagggc gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ctgagcgtct
     5281 gtgtactggt gatgcgtctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca
     5341 cactatcggc aacccgttat accactggac gcacctcgaa ctgcgtcgtc cgttcggtat
     5401 cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact
     5461 gctggcgcag gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt
     5521 cggcaccacc gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg
     5581 ctctttcacc atcaaagtgc tgccgagctg gcgtccggac aaagccttca atatcgaaca
     5641 ggcgaccttt aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg
     5701 ctttgctgac ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg
     5761 taaagtgtct gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact
     5821 ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcaca
     5881 gttcaaaact gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca
     5941 gcagtaccac attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc
     6001 ggatgtaggc tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct
     6061 gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg
     6121 cgataacgaa gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa
     6181 aatgcagttc ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat
     6241 gacccaactg gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg
     6301 tagcttcctg tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg
     6361 tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa
     6421 aaatatttgc tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg
     6481 gttgatatgc aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat
     6541 ttggctgaag gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt
     6601 gctcgtggac ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat
     6661 ggcctgccga ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac
     6721 aatacgcgca cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat
     6781 ctgcctgcgc aagcggcaga tcctgaagtg cagatctatc gtcgcgctaa cggcgatgtc
     6841 ggggtgcgta acgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg
     6901 caaatccaga accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc
     6961 ctcttcagcc acacctatgg ctgctcacaa ctgggcgacg atcacatcaa cacccgcacc
     7021 atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc
     7081 tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc
     7141 gttcatttca tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg
     7201 catcagctgt ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg
     7261 aagtttggtc tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg
     7321 ctggggcgtt tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg
     7381 ccggagatgt ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt
     7441 gaaaaactgg tcaccatggt caacgacttc aaacagtact ttattgccca tgatcagccg
     7501 atctacgaaa acccatcacc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa
     7561 tcacttggct gtacccagaa agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc
     7621 gagcgtctga aaacgccagg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg
     7681 accagcgccc tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgcggcacg
     7741 ccatacggtg gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag
     7801 aaaaaacact ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag
     7861 ttgctggaag aatttatcga caccatcgtt gagtttgcca acggtaagca aacctgcaac
     7921 gagcgtaacg acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga
     7981 aatgagttgt taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac
     8041 ctgctaagga tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt
     8101 tctgattggt atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc
     8161 ggtctatagc gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc
     8221 cagtgccatc cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt
     8281 aatggcgatt tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt
     8341 cgaactattg ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg
     8401 gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg
     8461 ggcggggtcg ataggcggca cgatgattga gggtagtttt ctgcttatga atggcctgaa
     8521 tatcattcgt ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac
     8581 ccctcccgcc atagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga
     8641 cgatcgtctt ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc
     8701 gcggtttcgc aagagtcctg caatacgccg ataataaagc cgacggcaac cacctgcatg
     8761 gcgatatcgt tcgaaatacc gaacatatta caggccagtg ggatcagcag caaagaaccc
     8821 cccgccacgc cggatgcgcc acaggcacac agagaagcca ctacgctcaa cagcagcgct
     8881 gtgggcagat cgaccggaat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg
     8941 gtgattgctg cgcccgccat attgatggtg gctcccagcg gaatagaaac tgaataggta
     9001 tcgcgatcca gattcagctt ttcacacagc gccatattca ccggaatgtt cgctgcagag
     9061 ctgcgggtga agaaggcata cacaccgctt tcgcgcaggc acagcagcac cagcgggaac
     9121 gggttacgac gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac
     9181 atacagccaa ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg
     9241 gttgccaggg tagaagaaac cagcccaaaa ataccgaacg gtgcgaagcg aatgaccagt
     9301 ttcaccataa aggtaacggc attcgacata tcattgacca ggtttttggt ggtctcgtta
     9361 ccgtgacgca gtgcgaagcc gaggccgatc gcccacacca gaatcccgat gtagttacct
     9421 ttcagcaggg cgtcgatggg gttggaaacc atgctcatta ccagtccgcg catcacttcg
     9481 acaatgcctg acggcggcga aatatcaccc gcgctactgg acaagtgcag ggtagaaggg
     9541 aaggcaaagc tgaagactac tgcggccaga gctgcagaga aggtgcccag cagatagagg
     9601 aacaaaatag gacggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatt
     9661 accagcatca acaccaggat gggggcaaca gctttcagtg cgccgacgaa caaagtacct
     9721 aacagaccaa cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca
     9781 aggccgacca ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt
     9841 gaacgttgcg tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt
     9901 ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt
     9961 acagcttcgg gaagcgaaaa atgctggatt ttaaagagag gtcatgtttt gcaacttaat
    10021 ggttgcattt atttaacata aacagaggga aggactggct actaaggctg attagataac
    10081 atatgggcaa aactatttgg aatatgagaa cggttgcgct cctttaacgt gttatgccat
    10141 ataaagtcac tggcgcaggg agcgcgcagg gggcggccaa tcgccgccgc cccctgcacc
    10201 cccaggctct ggcgaacaaa atcgccgctg cgcggttccc tcagcctatc ccttgcggct
    10261 accgggtcgg gcgcaaggta acgtccctgt aagatgcgcc ctcgccccgg cctccaggga
    10321 acgcttcggc gattttgacg ccaccaacac ccgtgcggtc tttttgtaaa gagctataag
    10381 ccgattacat aattaatagc tcacctttgt ctggaatgag ttatctctag gaatggctta
    10441 atatacagcg aatcattctc cccgctgctt atcatgccga taattcaccc aggcgttgat
    10501 aataaacgtc atcaccagaa tgccaaacac cacgcccagc gagacggcga ttggaatatg
    10561 gtagaagtcg acaatcagca tcttgatacc gataaacacc agaatcaccg ccaggccata
    10621 tttgagcatc gagaaacgct ctgctacgcc cgccagcagg aaatacatcg cacgcaggcc
    10681 gaggattgca aacaggtttg aggtcagcac aatgaacggg tcagtggtca cggcgaagat
    10741 agccggaatg ctatccacgg caaaaatcac gtcgctcaat tccaccagaa tcagtaccag
    10801 catcagcggt gtggcgtaca gcaatccatt cttacgcaca aagaaatgct cgttgtcgat
    10861 ggtgtcggtc atgcgcaaat gaccgcgtag ccagcgcacc agcggcttgt cgccaatgcc
    10921 tgattcatct tcatgggcga gcgccatctt cacgccggta aacagcagga aggcaccgaa
    10981 gatatacagg atccagtcga actgcgaaat cagccagctg cctgtgaaga tcatgatggt
    11041 acgcagaaca atcgccccga gcacgccata caccagcacg cgacgttgta atgccgccgg
    11101 aacggagaaa tagctgaaca acatcagcca gacaaagacg ttatcgaccg ccagcgattt
    11161 ctcaatcaga taacccgtga gaaaggccag ggcctgtgga tcggcaacgg cgcgaccttc
    11221 ggtttgcacc agataccacc agaaagcggc gttaaacagt aacgacagcg tcacccagac
    11281 cagcgaccag gccgcagcct gtttcatggt catggcatgt gccccacgac gcccctgcaa
    11341 caacaggtcg atagccagca taatggcgac aacaacagcg aatccgcccc atagcaacgg
    11401 cgtgccgaca gtattcatag aagttcctta cacataaaaa aacggccaac gtcggaagac
    11461 gtcagccgct gctttttatg catagacctc gccttccggc aaggtctcac ttacaacaaa
    11521 aaaggaatac gtctgcgtat tcctttcggg ttgcccggtg accggatgtg gtttttcaca
    11581 catcgtaatg acgatcgacc ggcaatgaag ttactcccct ttgcaggtaa caaagtatga
    11641 cagaccattc agtcagtcaa tggtctgtta catacctttt acactgtttt actttggcaa
    11701 gtatagtttt acgctatctg ccggaaaaat caccccagtt tggcggcgga tctcggtcag
    11761 cagtttggct gtgatgcgac tgaccgccag ccctggatga tccaccagat gctcatccac
    11821 cagggtagcg aacagctctg cttcgtagag catggtatta atatgctgcg gctgggtgag
    11881 atcctgcatc tggctgccac gcggcacaaa gcagactttc tggcattcag acagtttttc
    11941 aatcaccagc gatcccgctt cgccctgaat ctcgcttccc aggacagaat cactgacctt
    12001 tgagtgctgc aaggtgacgc tgaaatcacc gtaatccatc accaccacgc cgtgggcgtc
    12061 aacgccgctt gccagcaaac tcgccgttgc ctgcacgctt ttcggctcac caaataacgc
    12121 caccgccgac gccaggcagt aaaagccgat atccataatt gaaccgttag agaatgccgg
    12181 attaaaggtg ttgggattct caccgtccag gtaacgttga taccgcgagg aatactggca
    12241 atagttgaaa aagactttac gcagtttgcc tgctttcggc agcgcctggc gcaacaaatg
    12301 aaagttcggc aggcaggcgg ttttaaatgc ctcaaacagc accacctgat tttcgcgcgc
    12361 acaggcaatg gcggcatcca cttccgccag attcgacgcc agcggttttt cgcaaatcac
    12421 atgaatttta tggctaagga aaagttgtgt ctgggaaaag tgcagggaat tcgggctggc
    12481 aatatacacc gcgtcaatgg catcgctttc cgccatcgct tccagcgacg taaacagatg
    12541 ctcgacagaa aaatcattgg cgaagtgctg agcctgctca aggctgcggg aatatacggc
    12601 ggttaacttg tatttaccgc tctcatgggc ggcctcgacg aactggcgag tgatccagtt
    12661 cgtaccaatc acagcgaaac gtatcataac gcgtggatac tccataaagg gattctgtcg
    12721 tcaatgtagc acgccttttt catcttcgac gtgcgcagat acgcattatt ctttcgccgg
    12781 acagccatgc tcactaaacg gcccaatcat gaccgatggc ccgagatatt tcggctggcg
    12841 atggaggatt tttgcgtcaa tcaccgcgct gacacgagcg agtttctccc gcagttgagt
    12901 ataaaaaccg tgcttcatat tgaggtctgg cgcgttaaac ccgatagcgt cgatgccgta
    12961 ctgttttgcc agccagatgg cgcgttggtt atgaaattct tgcgagataa tggtgatatg
    13021 gttttcccca aacacctttt tggcacgcac taccgaatcc agcgttgaga atccggcata
    13081 gtcgcaaaag atcactttcg ccggtacgcc tttagcgatc aaagcctgct gcatacctga
    13141 tgcttcatcg taatttttac gtccgttatc gccactcacc agtagccatt tcacttttcc
    13201 ggcgtggtac aacgctgctg cggtatcaat acgtcgggta aagtagcgat tacccggcct
    13261 cgcccccagc aataaaccca cgttacgcgc cgggacggcg ttgacatcgc cccaggtcag
    13321 ctgcttgctg gcgttgacca taacgcgatc ggcgataaaa atggtcgctc ctgccacaag
    13381 taacagtaag caggcaatct ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg
    13441 ggcaaatgcg cgcagcatgg tcgttatcct taacgaaagg ggggaggtcg attttaggcg
    13501 ctaaagaaaa gataacgcct gacatcattt tcagattttg tcctgcccaa gcgacaactg
    13561 cgttagccac agccgggtgt cgaactcaag ctggtggtac tgcggctcca tatggcagca
    13621 caattgatag aacgctttgt tatgctcttt ctccttcagg tgcgccagct cgtgcaccac
    13681 gatcatgcgc aaaaacggtt ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc
    13741 ttttgctttc agcttgccgc cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt
    13801 ttttagcaca tggatcttat tgtcatacat cactttattg atcggcgggg cattacgcag
    13861 aaactgattt ttcagatcct gagtatattg ccagagagct ttatcggtgg cgtagtcgtg
    13921 tgttcccgga tagcgttttg ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg
    13981 cacctgcgaa agcagctgct cgggataacc ctgaagataa gtaagattgc tcataaccgc
    14041 ccacgaaaaa gagaaaaagg gtatactcac gcaccctttt caggggcaac gtcgaaattt
    14101 tatcattcag gagggccgat gagccactta gacaacggtt tccgttcact gacactacaa
    14161 cgttttccgg cgacggatga cgttaacccg ctacaggcgt gggaagcggc agatgaatat
    14221 ttgctgcaac agttggacga cacagaaatc cccggcccgg tgttgatcct gaatgatgcc
    14281 tttggtgcgt taagctgcgc gctggcggaa cataagccgt acagcattgg cgactcatac
    14341 atcagtgaac tggcgacgcg cgagaattta cgcctaaacg ggattgatga gtcgagcgtg
    14401 aagtttctcg acagcaccgc cgactacccg caacagccgg gtgtggtgct gatcaaagtg
    14461 ccgaaaacac tggcattgct ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgtcg
    14521 gatacacgta ttattgccgg tgccaaagcc cgtgacattc acacttccac gctggaactg
    14581 ttcgaaaaag tgctcggccc gaccaccacc acgctggcat ggaaaaaagc gcgcctgatt
    14641 aattgcacgt tcaatgaacc gccgctggcc gatgcgccgc agaccgttag ctggaagctg
    14701 gaaggtactg actggactat ccataaccat gcgaatgtct tctcccgcac cgggcttgat
    14761 atcggcgcgc gcttctttat gcaacatctg ccagagaatc tcgaagggga gattgtcgat
    14821 ctcggttgcg gtaatggcgt tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa
    14881 gtggtgtttg tcgatgaatc gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc
    14941 aacatgccag aggcgttgga tcgcagcgag tttatgatca acaacgcgct ctccggcgtg
    15001 gagcctttcc gctttaatgc tgtgctctgc aaccctccgt ttcaccaaca acatgcgctg
    15061 accgataacg tcgcctggga gatgttccac tacgcccgcc gctgcctgaa aatcaacggc
    15121 gagctgtata tcgttgccaa ccgccatctg gattacttcc ataaactgaa gaaaattttc
    15181 ggcaactgca ccactattgc gacgaataat aaatttgtgg tgctgaaagc ggtgaagctg
    15241 ggacgtcgtc ggtaagattg ggagttacct gccggatgcg gcgtaaacgc cttatccggc
    15301 ctacgttcag cacgttaccc ctaggccgga taagacgcgg caagcgtcgc atccggcaat
    15361 gtctgctcag ttctggaatc gcgcttccat aaaacaaaaa atccctgtca ccatcctcta
    15421 cattctcttg tttagcgttt ttctacgttt attcttccgt cacacagata aattcatccg
    15481 ttgccaatct gtcaacggat gttatcatat tgccaatttg tcaacggacg tgatgaagaa
    15541 tgcacctgat aactcaaaaa gcattgaaag atgctgcgga aaaatacccg caacataaaa
    15601 cggagttggt ggctctgggg aacacgattg ctaagggata tttcaaaaaa cctgagtcat
    15661 taaaagcagt attcccatct ctggataact tcaaatatct ggataagcat tatgttttca
    15721 atgttggggg caatgaatta cgtgttgtag caatggtctt ttttgaatcg caaaagtgct
    15781 acatacgtga agttatgacg cataaagaat acgatttctt taccgctgtt catcgtacta
    15841 aggggaaaaa atgattgcga ttgccgacat cttgcaagca ggagaaaagc taactgctgt
    15901 ggcacctttt ctggcgggta ttcagaacga ggaacaatac acccaggcgc tggaactggt
    15961 agatcatctg ctgctcaacg atcctgaaaa ccccttgctg gatctggtgt gtgccaaaat
    16021 aaccgcgtgg gaagaatcag cgcccgaatt tgcggaattt aatgccatgg ctcaagccat
    16081 gcctggcggt atagccgtga ttcgtaccct tatggatcaa tatggtttaa ccctttccga
    16141 tctgccggaa attggcagta aatctatggt gtcacgcgtt ttgagcggga agaggaaatt
    16201 aacgctggaa cacgctaaaa aattggcaac gcgattcggc atttctcccg ccttgtttat
    16261 tgattaacgc gacgtgccgg atgcgttgca tccggcgctt caaataacga tttaaatctc
    16321 cagcgccagc cgtgttccct gggcaattgc ccgtcgtgcg tccagctcca tagccacatc
    16381 gcagccgcca attaaatgca cagttttccc gctatcaatc agcggttgcg ccagcgcgcg
    16441 gtttggctct tgccctgcgc agatcaccac attgtccact gctaatacct gcgtttcgcc
    16501 gttgatcacc acatgcagcc cgtcatcgtc aatcttctga taacttacgc ctgggatcat
    16561 tttcacaccc cgcgagagca ggatggtgcg atggatccag ccggtggttt tgcctaaccc
    16621 ctgtcccggt ttgctggctt tgcgctggag catcacaatt tgccgtgggc tacgcgggat
    16681 ctgcattccc tgcgggctta agccaccagc ctgttgtagg ctgctgtcga tcccccactc
    16741 attacagaac ctggcgatat tctggctggt ggattcgccc ggctgactta aatacatcgc
    16801 cgtatcaaaa ccaatcccgc cacaaccgat gatggcaacc ttgttgccta ccggcgcttt
    16861 gtcgcgcagt acatcgagat aactcaatac cttcggatga tcgatcccgt cgataggcgg
    16921 aacacgcggc acgatcccac tggcgaggat cgtttcatcg aacgcctgta actgatccgc
    16981 cgtcacggtg tgattgagtt tcagcgtcac gcccgtcact tcgatcatcc ggcggtagta
    17041 gcgcagcgtt tcgtaaaact cctctttgcc ggggatctgt ttggcgatat taaactgccc
    17101 gccgatctcg ctgtgcgcat caaacaatgt tacctgatgc ccacgcgccg ccgcgttaat
    17161 ggcaaacgcc agcccagcag gtccagcgcc gaccaccgcc agattttttt tctgcacggc
    17221 gggaaggatt ggcattttgg tttcgtggca ggcgcgagga ttcaccaggc acgaggtgac
    17281 tttgccaacg aagatctgat cgaggcagcc ctggttgcag ccaatacaag tgttgatctc
    17341 atcggctcgc cctgattgcg cttttgacag cagttccgca tccgcgagaa acggtcgcgc
    17401 catcgatact atatcggcat cgccgcgcga gagaatatcg tcggcaacct gcggatcgtt
    17461 aatccggttg gtggttacca gcggcagcga gacgtggcct ttcagtttgc gcgtgaccca
    17521 gctaaatgcg ccgcgcggca cgggcgtggc gatggtcgga atccgcgctt cgtgccagcc
    17581 tattccggtg ttgataatgg tcgcgcccgc cgcttcaatg gcctgcgcca gttctaccgt
    17641 ttcggcaaaa gtgccgccgc cttcgaccag gtcgagcatc gacaaccggt aaataataat
    17701 gaagtcgttg ccgacgcgtt cgcgcaccgt acgcactact tctacggcaa atcgcatccg
    17761 gttgcggtaa tcgccgcccc actggtcact acgctgattg gtgcgcagcg tcagaaattc
    17821 gttgatcaaa tacccttcag agcccatcac ttcgacgcca tcgtatccgg cctcccgcgc
    17881 cagttgcgcg cagcgggcga aatcgtcgat cagttgcagg atctcttcat gggtgagttc
    17941 gtgaggaacg aaacggttga tgggggcctg caatgcggac ggtgcgacca gatgcggttg
    18001 gtagctgtag cgcccggtat gcaaaatttg cagggcgatt ttgccgcctt cctgatgtac
    18061 ggcttcggta atggtgcgat ggtgtgggat ctggctggcg tcgttgagca ttgcgccacc
    18121 ttccatgcca acgcctgtta aatctggtgc aatgccgccg ctgacaatca gcgccacgcc
    18181 gtgacgggcg cgttcggcat aaaacgcagc cagccgttca gcaccgtccg ggtattcctc
    18241 cagcccggtg tgcattgaac ccatcaacac gcggtttttt aacgtggtaa aacctaaatc
    18301 cagcggggcg aacagcgacg ggtagctcat aaaattgtcc agtatgtaaa ataattgtta
    18361 tgtagtcgga tgagttctaa tttagccgtc gatggagaaa agggaaaagg ggagtgcggg
    18421 gtttgtgatg ggattcaaaa agaatacgcc cggtggtaac gccggatgcg acgcttgccg
    18481 tgttttatcc ggcttggggg attgcacatg tagggcggat aaggcgttca cgccgcatcc
    18541 gccagccgtt gcaaattact gcttacggaa aaaatcgtta tacaacatat acaaatgagc
    18601 ggcactccag gagaaatttg gtgcgccttg ctgcgcgcct gtcagcgggt tgtaattctc
    18661 ctgaattggg ccatcggcgg ttaatccttt ggcgtgcctg aagaacgtat ccgccagctt
    18721 cagggcatca tcacgataac cgtaacgctc catccctttc agaccaaacc agaactgatc
    18781 cacccatacg cgcccgcgcc agtagatatc agcgccaaaa gccggattgg ttaacgccgc
    18841 cgttcccagc ggaacaaagg tgttgaactc tttaggatcg agcatcacct tcaccaccgc
    18901 gtcggcattg gcctgcgttg ccgcaccgtt aaacagcggc gaccagcctt ccggcccttt
    18961 accgcgctca acaatcggtt tgcccgcgca gccgttcgcc agtggtttat cttcaatacg
    19021 tacgtcatag tagaactggg tagtcgggtc gaacatacag gtgttgatgt agtccgcgag
    19081 ctgctgtgcc aactggcgat agcgtttggc ctcttctggc ttaccgagga tggttgccat
    19141 ctccgccaga taatggttat cgctgtacat atagctggcc tgatccaccg actcctgcaa
    19201 tagcgagtag cccagcaacg ttccgtcctg actgcggttt tcggcgaatt tcaccgtcca
    19261 gtcgctacgt ttgccgccat tagcgacata tttatccaac tgttctttgt cgataaaacc
    19321 aaagacggcg gcatcatcac ggcccgattc ccacgacgca gcgacctgtg ccggaatttc
    19381 cagactgtcg tactggcctt tctccaccac gcgggcgtag ttgttcagcc cagactgcgt
    19441 ctcttcttta tcgccttttt tcaccgtaaa cagcatctcg ccgctctcgg tgttgtgggc
    19501 tttgtcgcgg gtcgcgccat attccggcac cccgttgccg ttatgatcgc ggttacgtaa
    19561 ccaccagtcg tgataggcca ccagtttcgg gtacatctct gccaaccagg ctttatcctg
    19621 ggtgacgttg tatacttcca tcaccgacca ggcggcaagg ctgggtttgg tattacgttc
    19681 gttccagttg ccgccatcgc caccacgctc ggggctaagg ttccacgcta tcaggtcggg
    19741 gacaaagccc acatcctgcg gacgcacgct gtcgccaggc tggatctgcc aggagaagac
    19801 cgcgcggata ttctctttgg cgatatccgg attgaaatgc gccatcgcaa acgcctgctt
    19861 ccaggtatcc cacggccagg tctgattgcc ggagaaccag cgtccggtca ccgacggcgt
    19921 gacggtgtta taattcaccg caccgcccgg tgagcgccag ttaccgttga gcgtttcgat
    19981 cgctttcacc gcgacgcgcg tctgttccgg cgtcgcatcc ggattggtta accctttctt
    20041 caggtattct tcccagcgtt gctgcgaggc ggtgagataa aacgccggac gcgccagaat
    20101 atcgcggatc tgcatttgct ctttgctaac ttcctgagcg gtcagcagat gggaataggt
    20161 ggtgtagagc gtggtcgaac cgttgatatg cgccttactg gtaaagcgat taccgttgat
    20221 ttcagtctgc accggcaggg atttatgcac ctgatattcc gattcgccgg aggtcagcag
    20281 atcccaggtg gcgcgcactt tgccaaaggt gactttcagg ccatcacggg tggcgctgat
    20341 tttgcgctga tagtcagggt attcgccagc aatggtttta tcggaaagcg gtttcccttc
    20401 tttcgcttcc agtttttcca gcagttcgcc atcccacacc agatccagcg gtttattgct
    20461 gatgatttta gtttccagta gtgacgtgcg cggcgtggcg aagcgcagag tcatttcgac
    20521 ctgcacatct tttgctgtca gtttttgcac cagcgcgccg ggaatactgt atgcctccag
    20581 cgtgaagttg actttcttgc cgtcttgcca gacagtcagg cggtcgaaat tgctggccat
    20641 aaagttgatg tattcttccg tcagcagcgc aacgcccgga aagccgccca tggtgttcgg
    20701 gccgtctggc aacagatgac catgccaggc tccgagatcg aaaaacggat taaaacgctg
    20761 gtgatcgtcg taatcgtaat ccttcatgta ctgcggcgca ccagtacggt taatcacgtt
    20821 tttataattg tcggcgttag cggcatgagc ggaaaaactt atcagcagag cgcaggttac
    20881 tggcgttaaa atagttttga ttttcatgag attacagtcc ttattaccag tagaaatatt
    20941 gcatcaggaa gatttggtcg ctgggatcgt tagaggcatt tttcatgtag aagcggctat
    21001 caaaagccgt ggcgaaacgt ccgccgccat attcgtacca gataccaaac tgcatgaaag
    21061 aggtggtttc atcgtcagcg tggtcagggc gatgtttggc gtaggtgtaa gcggtggaga
    21121 gataaacgcc ttttaacgct tcatacatgg cgctcaccat catcccgctt tcacttcccg
    21181 gtacgtccag cccgtcggca cgtccggtat ggtgccaggc gcgagcggca aagttagggg
    21241 taaacaggcc cgtgagatag agttgtcctg tgccgtcggt aatttccaga ccattcatcc
    21301 aggtgatgcc ttcggcaagg tcatactgga tgtaaccgtt catccattgc cggataggtg
    21361 tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga
    21421 tgtccggcag gtgtggcggt gacgctataa cgaaagttgc cgcttaagtt ctgtaccttc
    21481 agcgcggtca taatgtcgcg ggtattggga atgacgtagc ccaaatcggg tgtgaagtcg
    21541 ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg
    21601 ccatcattgg tacgataacc gccatagaaa cggttaatcc caccttcgaa accaccccag
    21661 ctatggtcag gcacctaggc attaccgtcg tggtcggcct gcacggtcca gccttcgccg
    21721 taaatcaggc cgaaccagga gccgtacttc atctccaggc caccttcaat ataggtgccg
    21781 tcgctgtatt tatgtttatt ctcaccctct aaatccatat atccaccaag ccctaattcg
    21841 ccgtaaaaac gaaatgaagg agaagatgtc gaggtaattg cgggtgtttt tgtgtcgtca
    21901 tgttctgccg gacgtgcttc ttctcctgcc gcagaaaagg cgtagcaaaa cggcagagca
    21961 agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa
    22021 aagtacagcc ttttatcgtt gtaattataa aaatataaag gcagaaacta ttttccctct
    22081 cctcatccag gaagagggaa aatgcctgct cattttttat tagtctgctt aatcaacatt
    22141 gctggctggc gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta tatatttgct
    22201 gtatttccac cacgcaaagc cgaggaagat aacaataccg ccgacgttat aaaaaatgat
    22261 ggtcagaata ttcgcgccag tcgggaatgt cgaagcgaca aaccctacgg caaatatcgc
    22321 aatcagcatt gaaacaacga taataccggt gcggcgggag cccatacgga aatcgcgtgg
    22381 caggtgatct aatttggcgc gtaaattcag gtaagccagc atgatgaata acggcggaag
    22441 catggacgct gcggcggtca tattaataat ggtattcatc agatcctgca cggtattgga
    22501 accgagcatc gggataatca tcagcgggat gacgatcaga aactggatcc atgctgcgcg
    22561 cgccggaacg ccgttttcgt taagttcgac ggttttctta ccaaagatgc cttccgggat
    22621 ttcggagaag aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacattgc
    22681 ggtaaaggac accagaccga caaagcgatt catcaacgct tccggcagac caaaatacgc
    22741 cgccatgccg tggaatacct gcaccgatcc gccggtaaat ttcaactctt tgctgctgac
    22801 gaagacgtta atcagcaccg aggagacgga atacagtacg ccgataaaaa tcccggcgag
    22861 gatgatcact ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acaccgcgac
    22921 ggactccgcg ccacctgcgg ccataaagat ccaggtggta acgccgagga acgcccagtt
    22981 gaagttcggg atcatcgcgt caacggtgat cggatcggca ggctgtacgc cgccaaccag
    23041 tgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
    23101 ggaggtaatt ggccccagca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
    23161 cagcaccata ctgataacgg tggtggcaac cggcgtcata atatattcgt agccgaggaa
    23221 ggcatacgaa gcataagcaa taacgcgcgg caacagcgag gtgaaaaaga acagatttac
    23281 gaaccagtaa gtgtaggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
    23341 cgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
    23401 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
    23461 ctcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
    23521 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
    23581 tgtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
    23641 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
    23701 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc tacggtttct
    23761 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
    23821 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
    23881 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt cattttccac acggtaggtc
    23941 aatgaatcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
    24001 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
    24061 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
    24121 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
    24181 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
    24241 agttggagcc gaggccaagt agctggtggt cgaggttcag ggtgatgtca tcactgcgct
    24301 gcagctcgtt acagtgctgg gcagcgtgga tgttttcctg ggtatagtgc caggcgctga
    24361 agttaattgg gcgctgcgga accaccagca gaccgttgcc gtggcggtta gtcagtgccg
    24421 tccagcggac atgctgacgg ttgccgttgt tctgcgggaa gggatagttc tcgaacatgg
    24481 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
    24541 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatc cccatggtga
    24601 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
    24661 taacctggcc atcggcagcg atgcgccaga tgtaggtaca gcgcatcccg aagtcaaaca
    24721 ccggcggtgc aataactgtg cggctgatga tcaacacttc accatcgctc tgttctacgg
    24781 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac aacccttcgt
    24841 actcctgctt gtggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
    24901 gcagcgactc gccatttacc tggcaggatg tcggtttgcc actcattttt gagaaggtga
    24961 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
    25021 gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt ttccttcagc gggaactgat
    25081 aagaggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
    25141 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
    25201 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
    25261 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
    25321 tcaactcgcc gcgagtcagc gcgtggattt ttaccggcgc gataacctgt ttgtactctt
    25381 tcaggcccga tcccggcgtc tgatcggaat agatcaaacc atcaagacag aagttatagt
    25441 tgttgggata gtcgccgccg aatttatacc agacattgcc gtggtcgtcc tgtgcctgga
    25501 tcccgtggtc gcaccactcc cagacataat gaccctgaat gcaatcgtgc ttatagaaga
    25561 cgttctggta ctccgtcagc ccgcccggtc cgttccccat cgcatgagca tattcacaga
    25621 tgatgcgcgg cttcggatgc gggtattcac caaactcatt catcagcggc acgcgggtgt
    25681 acatggtgga aataatatcg accacttcag catcgcgatc ttcttcgtaa tgaaccagtc
    25741 gcgtgtcatc cagcgctttc gccgcatggt acatcgcgcg gatgttacag ccatagccgg
    25801 attcattgcc cagcgaccag atgatgatcg acggatggtt tttctgtgcg tggatatggc
    25861 gaacaatgcg ctcgacgtag actttttccc actgcggatc gtcggtaata cggctgatat
    25921 cgccgacatt agcaaagccg tgcgattcga cgtcggtttc cgccatcaca aacaggccgt
    25981 agatatcaca cagttcgtaa aaacgcggat cgttcgggta gtgagcggta cgcacggagt
    26041 tgatattgtg ctgcttcatc aactggagat ctttctcgac gcgatccatt ccaacggcgc
    26101 ggcctttgcg atgatcgttg tcgtgacggt tgacgccgtg cagcatcaca taacggttat
    26161 tgatccagaa cagaccgtcg cgcactttga tatcacggaa gccaacgcgt tgtggcacca
    26221 cttccagaac gttgccgttg gcgtctttca gcgtcatgac cagatggtaa agataagggg
    26281 attctgctga ccattgctgc ggctgttcga cagtaaaagc aaagctggcg ctggtcagtt
    26341 tttcaattgc caaatgatca atggcgctgc tgtgcaccac gcgttcgcca tcaaacaggg
    26401 tatattccag cgtcgtgacg acaggggagg cggcgagatt ttccagcacc acttcgcagg
    26461 aaagcgtggc atcgcaatag gcttcgtcaa agtcggtacg cacagtgaaa tcgttaatat
    26521 gcgttaggtg ttttccgacc agataaacat cgcggaagat ccccgccgac caccacatat
    26581 cctggtcttc cacgtaggta gagtccgccc actgcatcac gcgcacacac aacaggttgt
    26641 cgccggtttt aaccatcgcg ctgatgtcaa actctgcggt caggcgactg cccttgctga
    26701 aacccacata ctgaccgtta acgtagactt caaaataggt ttcgacgccg tcaaatttaa
    26761 tcagcgtctg tttaccctgc cagccgtcgc tgagggtgaa aatacgttga taggcaccgg
    26821 ttgggttatc gctggggaca aacggcacat cgatggggaa cggaaaacct tcgtcggtat
    26881 attgcagttt gccgtgacct tccatttgcc acatggcggg gacggtaata tgcccccagt
    26941 cagccattaa ctcagaggtg aaggcttctg gtacttgcag cggatggtca aaaaagtgga
    27001 aattccactg actgcttaag ggcagaaaca ggctgctggt ttcgcgggca aaggtacgcg
    27061 cttgcgcaac agaatcatat gaaaaaaagt acgcacgcgg cgcaagtcgg ttttcgtggg
    27121 tgagctgaat gttttcccag cgattcatag ggcctcccgg tagagagaaa tcggaaccat
    27181 tcatggtgat ggtagtttgt gtaattgctg gggggttagt aaaattttta ctaaaaagca
    27241 gcaggtaaat gcatttactt taactggatc acgaaaataa acggcagcac tgtccggcaa
    27301 aaatgattgc cggacagtgt gtaaggggat ttaacgggtc gtgccgcgca gttttaattt
    27361 gctgggaacg aagactagca gcggcagcgc gcgaccatcg cgggcttttt catacaccag
    27421 gttaacgccc tgacttccca tcatctcgga atggatgcgc acggtggaga gcggcggaaa
    27481 ggtaaatcgc gcggtgggga tatcgttaac gctgataagc gaaatatctt gtgggatgtt
    27541 caggccacgt tcatgaattg cccgcagtac gccgatagca atggaatcgg aagcaacaaa
    27601 cagtgccttc ggatagtctt cccgcgccag catttgtttt gccagttcat aacccgacga
    27661 actggaaaaa ccgccgcgcc agatatcctc ttcgcgtacc acttgtttca gtcggccata
    27721 ttccgcaaag gcgacctcac gaatatccgc cttgccaggc tcatcttcac cgccaataaa
    27781 accaatacga ttaacgccct ggttgatata gaagtcgatg atttctttac tgatgcgtgc
    27841 cagatcgata tccaccgcat cgtaaccgct gccgggttcg tgaaagtcga taaaacagat
    27901 attgtcggtc aacgcgctgg cagcggcgcg cagggcgggc gtgggtttgc cgacaattaa
    27961 aataccggtg acgtttttaa tgtctggtaa tccgttgtgt tcataacagt tggtcagttc
    28021 gatcgccagc ttttcgcact gagtttcaat gccgtggcgg atcgccagat agtaaggatc
    28081 gttgatctcc agctcctgct ggtagctgta gatagccaga atatggtgtt ggttgactgc
    28141 gcctgtctgt agtttacggg cgctactggt cttgtactcc agcttttcgg cgatctcgag
    28201 aatgcgatgt ttcgtctctt ctttcacatt caatgtcgga tcgtcattta agaccctgga
    28261 tactgtcgcc agggatacgc cagcttcgat tgcgatgtct tttaatgttg ccatgtttac
    28321 cttttttact ttccctgatg tgcgccccca ttgtagagga agtgcttatc gggtcacctg
    28381 ttttcagtaa ataaagcgcg tttctttgac gctgtaacgg taacacagcg gcttaggaat
    28441 gtgatcacgc ctgcattctc catttttagc ctacaggatt gagaaaaact tttctatagt
    28501 ttagtaaaat tttatccgaa gggaggaagc aatggaaacc gtggcttacg ctgattttgc
    28561 acgtctggaa atgcgcgtcg gaaagattgt ggaagtgaaa cgccatgaaa acgccgacaa
    28621 gctgtacatc gtacaggttg atgtggggaa aaaacactgc aaaccgtgac cagcctggtg
    28681 ccgtactaca gcgaagagga gctgatgggg aaaacggtgg tggtcttatg caatctgcaa
    28741 aaagcgaaaa tgcgtggtga aacgtcggaa tgcatgctgt tgtgtgcgga aaccgatgac
    28801 ggcagtgaaa gcgtgttgtt aacgccggag cggatgatgc ctgcgggcgt acgcgtcgta
    28861 taaaaagatc ggatggcgac gtcgtatcgc catccgattt gatattacgc ttcttcgaca
    28921 cttactcgca tggccgccag cgccttacgc gccgctttga tcaccagttc acactgttca
    28981 atggtcagtg tcagtggcgg ttcaatgcgg atcgttttgg cgttattgag cgttccggcc
    29041 accagtacgc gctggcggaa catctcgctg gcaaagttat agccgatttc gttatcaaca
    29101 aactcaatcg ccatcaacat ccctttacca cgcgcttcct gtaccagatc gggatattcc
    29161 cgcgccagtt gacggaaacc gtccagcaac atatcgcctt tttgctcagc ctgagccggt
    29221 aagttctgct ccagcaacac attgatggtc gccagcgccg ccgcacaggc cagcgggttg
    29281 ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca gaactgaaaa cacctcttca
    29341 gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga gggcttttgc cagacacaga
    29401 atgtccggct gaacgttctc atgttcgcag gcaaacatct tgcccgtgcg ccccatgccc
    29461 gtttgtactt catcgaggat catcagtgcg ccgaactcat cgcacagctt acgcacggcg
    29521 gtgagatagc ccggcggcgg cagaattacg ccaccttcgc cctgaatcgg ttcgaggatc
    29581 accgcagcca catcatcacc ggtttttttg cactcattaa gagccgtgcg catggcttcg
    29641 atattgccaa acggcacatg acggaaccct ggcagcagtg gcataaacgg tttgcggaag
    29701 gtcgatttcg ccgtggccga cagcgcgcca agtgatttac cgtggaacgc gccgctggtg
    29761 gcaataaaag taaatttgcc gcgcggcgac tgataagcct tcgccagctt cagcgccgct
    29821 tcaacggact cggtgccgct attacagaag aagctgtatt tcagtttacc gggcgttagc
    29881 gcagcaaggg ttttcgccag catagcccgt aacggatcga gtagctcctg gctgtgcagt
    29941 ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa ctggattacg gtgccccacg
    30001 ttgaaaattc caaaacctcc caggcagtcg ataaactcct gtccctgggt gtcgacaagc
    30061 gtatttaaac ctcccgcttg ccactctacg gctccgtaat ccccgccggc ggtaacagat
    30121 ttgcgatact ctaaaaaccc cggattgaca tgctctttga agtattcaat cacctctcgg
    30181 ttaagtgctt tcatctcctc atgatccagc gttcgcttct caatgagatt cagggcgtgg
    30241 gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt tcaaaatatg ctccggaggc
    30301 tcgcgtatca catgataccg atttaagtat tgcagggatt gcgccactcc ggctgcgatc
    30361 gcgaaaatcg ggataaacac aaatgcggtt atcatttgtg caatatttaa tgctatccgt
    30421 taacatttgt tgttattttg cacggttttt gtcgggtatg gatttgatga ttgctgcgca
    30481 aatgcacgtt ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt
    30541 atagtaacag agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat
    30601 tgcgatctaa atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg
    30661 acaagaagtt aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg
    30721 tcacccagca aaataccccg ctggcggacg ataccactct gatgtccact accgatctgc
    30781 aaagctatat cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag
    30841 agttacaagg gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg
    30901 cggatatgtg gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc
    30961 gcaaaaatgg cgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa
    31021 aaatcagtgg ctatatgtcg attcgtaccc gggcgacgga tgaagagatt gcggcggtgg
    31081 agccgctgta caaagcgttg aactccggac gtaccagtaa gcgtattcat aaaggcctgg
    31141 tggtgcgtaa aggctggctg ggtaaactgc cttcattacc gcttcgctgg cggacgcgtg
    31201 gagtgatgac cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg
    31261 tggtgacgta tttcctctgt gtgttagtgg tattgttggc aagcgcttgt tttgaatggc
    31321 agattgtgcg cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc
    31381 gtaatagtgt tgagcacctg aatcgcagcg atgagctggg gctgacatta cgcgcggtag
    31441 ggcagcttgg cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg
    31501 tcagaaatgg cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc
    31561 agacagttga taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga
    31621 aacagaactc tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg
    31681 cagtgcaggg tggggaggcg atgaccacgg tgatcaagac aatggacgat atcgccgaca
    31741 gtacctagcg cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata
    31801 ttctggccct gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg
    31861 cagtggtggc aggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata
    31921 ttcgcaagct gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacacg
    31981 ccgccggacg gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg
    32041 cccagattag ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag
    32101 tggatgagct taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg
    32161 tgtcggcgat ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt
    32221 aatcggtatg ccggatccgg cggttggagc acaatgcctg atgcgatgct ggcgcatctt
    32281 atcaggcctg cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc
    32341 cggcaattgc accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa
    32401 gcggcgcggt cgatcaccgc aatgatcttt tttatttgtg catcactgat atcgctctgg
    32461 ttgacgcgta aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc
    32521 tggcgcagcg ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacttgt
    32581 tcgcggtttt cttccagcca ctgcgcacct tgttcggtca gcgcaatctg cttcttacca
    32641 ccttcctctt cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgtcgggtag
    32701 atgacgcccg ggcttggggt gtaattcccc tgggttagat tctcaatcgc tttaatcaat
    32761 tcgtaaccgt ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg
    32821 tgaccaaaga aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcaacattga
    32881 ccgtgttcat gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag
    32941 cagccctcat gccgtggctg gccttcatgt ttacaacacc cttcgtgatg atggctcata
    33001 tcagcctcct tttgcttgat tagatatatc taatttatat ctgatgtgag cattactgca
    33061 agtacaaaaa tgatttttaa acgtggaagc acctcacatc taggccggat aagacgttca
    33121 cgccgcatcc ggcagcattg ctatggcact gaatgccttc acatacttca gacatatcag
    33181 ttctacaaat cgcttgcatt tatcatgatt aacaatcatt atcatttgcg aattttattt
    33241 agatatatct gattaccatc acgaaggcga taacaatgaa taacaccccc cgctacccgc
    33301 agcgcgttcg caatgatctg cgcttccgtg aactgactgt gctacgcgtt gagcgcatca
    33361 gcgccggttt tcagcgcatt gtccttggcg gcgaggcgtt ggacggtttt acatcgcgcg
    33421 gctttgacga tcacagcaaa ctcttctttc ctcaacctga cgctcacttt gtgccgccaa
    33481 cggtaacgga agagagcatc gtctggccgg aaggaccacg cccaccgtcg cgtgactata
    33541 cgccactgta tgacgaacta cgccatgaac tggcgatcga tttctttatt cacgacggtg
    33601 gggtcgccag cggctgggcg ataaacttac ggtggcaggt ccgcgcggtt cgctggtggt
    33661 gccggaagat tacgcgtatc agctgtatgt ctgcgatgaa tccggaatgc ccgcactgcg
    33721 ccgccgcctg gaaacgttga gcaaacttgc cgttaaaccg caagttagtg cgctggttag
    33781 cgtgcgggat aacgcctgtc aggattatct cgcgcacctt gatggtttta atatcgaatg
    33841 gctggcacat gatgagcagg cggtagatgc gcgtctggcg cagatgcaaa tccctgccga
    33901 tgattacttc atctggataa ccggcgaagg taaagtcgtt aagaatttaa gccgccgctt
    33961 tgaagcggaa cagtatgacc cacagcgggt acgtgcagcg gcttactggc acgcaaagta
    34021 actgatatat aaaaagaaaa aggctgacga tttctcgtca gcctttgcta tatctggtgg
    34081 cccctgctgg acttgaacca gcgaccaagc gattatgagt cgcctgctct aaccactgag
    34141 ctaaggggcc atggtagcgg attataaagt aactccgtgt cgcaatccag ccattaccgc
    34201 gcgcctgctg tttttataaa caatgtattt tcaatccgtt atacttttcc tggtgatgtt
    34261 agaaaggagt aaatatggtt gaggatattt tggctccagg gttacgggtc gtgttttgcg
    34321 gtatcaaccc tgggctttca tccgccggga ctggttttcc ctttgctcat ccggcaaatc
    34381 gcttctggaa ggtgatatat caggccgggt ttaccgaccg tcagttgaag ccgcaggagg
    34441 cacagcatct gctggattat cgttgtggca tcaccaaact ggtagaccgt ccaacggtgc
    34501 aagccaatga agtttcaaag caggagctgc acgcaggtgg gcgtaagctg attgaaaaaa
    34561 ttgaagatta tcagccgcag gcgttggcga ttctgggcaa acaagcatat gaacagggat
    34621 tcagctagcg cggtgcacag tgggggaaac aaacgctcac cattggttcg acgcagattt
    34681 gggtgctgcc aaa
//
LOCUS       DAJTUN010000030        34455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTUN010000030 DAJTUN010000000
VERSION     DAJTUN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.030"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..332)
                     /locus_tag="OO470_001384"
     CDS             complement(<1..332)
                     /locus_tag="OO470_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250683.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            712..1662
                     /gene="corA"
                     /locus_tag="OO470_001385"
     CDS             712..1662
                     /gene="corA"
                     /locus_tag="OO470_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6250684.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1703..2083)
                     /locus_tag="OO470_001386"
     CDS             complement(1703..2083)
                     /locus_tag="OO470_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6250685.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2097..2513)
                     /locus_tag="OO470_001387"
     CDS             complement(2097..2513)
                     /locus_tag="OO470_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250686.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2572..3462)
                     /gene="rarD"
                     /locus_tag="OO470_001388"
     CDS             complement(2572..3462)
                     /gene="rarD"
                     /locus_tag="OO470_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6250687.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3514..3981)
                     /locus_tag="OO470_001389"
     CDS             complement(3514..3981)
                     /locus_tag="OO470_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6250688.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4146..5015
                     /gene="pldA"
                     /locus_tag="OO470_001390"
     CDS             4146..5015
                     /gene="pldA"
                     /locus_tag="OO470_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6250689.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5148..6977
                     /gene="recQ"
                     /locus_tag="OO470_001391"
     CDS             5148..6977
                     /gene="recQ"
                     /locus_tag="OO470_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6250690.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7041..7661
                     /gene="rhtC"
                     /locus_tag="OO470_001392"
     CDS             7041..7661
                     /gene="rhtC"
                     /locus_tag="OO470_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6250691.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7723..8343)
                     /gene="rhtB"
                     /locus_tag="OO470_001393"
     CDS             complement(7723..8343)
                     /gene="rhtB"
                     /locus_tag="OO470_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6250692.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8454..9476
                     /gene="pldB"
                     /locus_tag="OO470_001394"
     CDS             8454..9476
                     /gene="pldB"
                     /locus_tag="OO470_001394"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6250693.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9484..10284
                     /gene="yigL"
                     /locus_tag="OO470_001395"
     CDS             9484..10284
                     /gene="yigL"
                     /locus_tag="OO470_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6250694.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10360..11259
                     /gene="bioP"
                     /locus_tag="OO470_001396"
     CDS             10360..11259
                     /gene="bioP"
                     /locus_tag="OO470_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6250695.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11147..12100)
                     /gene="metR"
                     /locus_tag="OO470_001397"
     CDS             complement(11147..12100)
                     /gene="metR"
                     /locus_tag="OO470_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6250696.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12336..14597
                     /gene="metE"
                     /locus_tag="OO470_001398"
     CDS             12336..14597
                     /gene="metE"
                     /locus_tag="OO470_001398"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6250697.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14636..15451)
                     /locus_tag="OO470_001399"
     CDS             complement(14636..15451)
                     /locus_tag="OO470_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6250698.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15713..16474
                     /gene="udp"
                     /locus_tag="OO470_001400"
     CDS             15713..16474
                     /gene="udp"
                     /locus_tag="OO470_001400"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6250699.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16615..18042
                     /gene="rmuC"
                     /locus_tag="OO470_001401"
     CDS             16615..18042
                     /gene="rmuC"
                     /locus_tag="OO470_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6250700.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18137..18892
                     /gene="ubiE"
                     /locus_tag="OO470_001402"
     CDS             18137..18892
                     /gene="ubiE"
                     /locus_tag="OO470_001402"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6250701.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18906..19511
                     /gene="ubiJ"
                     /locus_tag="OO470_001403"
     CDS             18906..19511
                     /gene="ubiJ"
                     /locus_tag="OO470_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6250702.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19508..21148
                     /gene="ubiB"
                     /locus_tag="OO470_001404"
     CDS             19508..21148
                     /gene="ubiB"
                     /locus_tag="OO470_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6250703.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21227..21496
                     /gene="tatA"
                     /locus_tag="OO470_001405"
     CDS             21227..21496
                     /gene="tatA"
                     /locus_tag="OO470_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6250704.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21500..22015
                     /gene="tatB"
                     /locus_tag="OO470_001406"
     CDS             21500..22015
                     /gene="tatB"
                     /locus_tag="OO470_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6250705.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22018..22794
                     /gene="tatC"
                     /locus_tag="OO470_001407"
     CDS             22018..22794
                     /gene="tatC"
                     /locus_tag="OO470_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6250706.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22836..23618
                     /gene="tatD"
                     /locus_tag="OO470_001408"
     CDS             22836..23618
                     /gene="tatD"
                     /locus_tag="OO470_001408"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6250707.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23615..24103)
                     /gene="rfaH"
                     /locus_tag="OO470_001409"
     CDS             complement(23615..24103)
                     /gene="rfaH"
                     /locus_tag="OO470_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6250708.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24270..25763
                     /gene="ubiD"
                     /locus_tag="OO470_001410"
     CDS             24270..25763
                     /gene="ubiD"
                     /locus_tag="OO470_001410"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6250709.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25809..26510
                     /gene="fre"
                     /locus_tag="OO470_001411"
     CDS             25809..26510
                     /gene="fre"
                     /locus_tag="OO470_001411"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6250710.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26702..27865)
                     /gene="fadA"
                     /locus_tag="OO470_001412"
     CDS             complement(26702..27865)
                     /gene="fadA"
                     /locus_tag="OO470_001412"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6250711.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27875..30064)
                     /gene="fadB"
                     /locus_tag="OO470_001413"
     CDS             complement(27875..30064)
                     /gene="fadB"
                     /locus_tag="OO470_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6250712.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30254..31585
                     /gene="pepQ"
                     /locus_tag="OO470_001414"
     CDS             30254..31585
                     /gene="pepQ"
                     /locus_tag="OO470_001414"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6250713.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31585..32199
                     /locus_tag="OO470_001415"
     CDS             31585..32199
                     /locus_tag="OO470_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6250714.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32238..33689
                     /gene="trkH"
                     /locus_tag="OO470_001416"
     CDS             32238..33689
                     /gene="trkH"
                     /locus_tag="OO470_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6250715.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33701..34246
                     /gene="hemG"
                     /locus_tag="OO470_001417"
     CDS             33701..34246
                     /gene="hemG"
                     /locus_tag="OO470_001417"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6250716.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8104 a         8703 c         9309 g         8339 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgtggtg ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg
      481 atcactctaa gaggacattc gccttggaca cacccagtag atactggctc actatcctgt
      541 catccaggat caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg
      601 acctcaattt ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt
      661 tctgtgtgcc caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc
      721 gcatttcaac tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt
      781 gtaaatgcag tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa
      841 tctgaacttg gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca
      901 cgtttctttg aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg
      961 gaagatcacg ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact
     1021 ctgcgtgagc gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg
     1081 atggtagacg gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag
     1141 ttggcagatg aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg
     1201 gaagggcatc agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat
     1261 atcggctgga aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg
     1321 cgtaaagcgc gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc
     1381 gaatccctgc tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg
     1441 gcaatgggtt ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc
     1501 gtcgtgttcc tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg
     1561 ccagaactga agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc
     1621 ctggcaccgt atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta
     1681 gcctggctaa gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct
     1741 cataaaacca tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt
     1801 taacgccaat taatgcacca acaaatagag atagaatgcc aagtaataga actataattg
     1861 cttttttcca taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat
     1921 tcatttgaat agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg
     1981 gtgttgctcc ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc
     2041 atttttcact taatgaatca tcgttcatat attccttact cataaatact ccataactat
     2101 tgttttgatg aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc
     2161 aaaaaaatca tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata
     2221 gagatgatat atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta
     2281 tcacggagtc gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact
     2341 ataacaagaa aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa
     2401 tttattaaaa taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg
     2461 ataaacatac gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata
     2521 tgcccactct catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga
     2581 cgttctacgc tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa
     2641 ggtgaaagtc accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag
     2701 gaacatcagc gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt
     2761 ggcagcggca gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag
     2821 taaattcagc gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa
     2881 caggtaaatc gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc
     2941 ttctaccgca atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag
     3001 cgcgataata ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag
     3061 aatcaccgcc agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac
     3121 aatgttcacc agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt
     3181 caccgcccag ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat
     3241 aaaaattttc tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct
     3301 catcagcacc accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc
     3361 gggcacgtag taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc
     3421 aagagcgagt aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta
     3481 attggtaaca gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc
     3541 actggcaata taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt
     3601 gcctgcacgc aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata
     3661 atcaacgcga agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact
     3721 gatggtttcg tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag
     3781 cgccgacgca atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg
     3841 ctgattttta aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa
     3901 tgcgcggtta aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc
     3961 agcggtcagt acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt
     4021 tatgcttctt tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa
     4081 gtatgtaaaa tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac
     4141 gaccgatgcg gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat
     4201 atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta
     4261 tcgccaatat gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact
     4321 acctcatcta cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg
     4381 cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc
     4441 gtgggatttt aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc
     4501 aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt
     4561 tcctcggttt tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg
     4621 ggtataacca cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt
     4681 atactcgcct gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg
     4741 tgggtaatac tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa
     4801 tcggctatca cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg
     4861 gctacggcgg cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata
     4921 ctcaggttta cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg
     4981 tcggtgtagg ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc
     5041 tggaatccag catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca
     5101 ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg
     5161 aagtgttgaa tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac
     5221 agtttcgccc cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg
     5281 ttgttatgcc cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa
     5341 acggccttac cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac
     5401 tacaggccaa cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac
     5461 ttgaagtgat gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac
     5521 gcctgatgct ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg
     5581 tcgatgaagc gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc
     5641 tcggtcagtt gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg
     5701 acgacaccac gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa
     5761 tcagcagttt tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg
     5821 atcagttgat gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca
     5881 gccgggcgaa agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg
     5941 cctatcatgc cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg
     6001 atgatctgca aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg
     6061 ttcgcttcgt ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg
     6121 gtcgcgccgg gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata
     6181 tggcgtggct gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc
     6241 gccacaaact caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat
     6301 tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg
     6361 atccgccgaa acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc
     6421 gtgtgaatca gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc
     6481 agcgtatccg cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa
     6541 gccatgaaca ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa
     6601 atattgccca gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg
     6661 aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga
     6721 aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat
     6781 cgattgccga tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg
     6841 agatggctga acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga
     6901 tgcgcaagct ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg
     6961 gcgacgacga agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt
     7021 ttcccccgcg agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg
     7081 tggcgcttat gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt
     7141 cccgtaaaga agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg
     7201 ggattgcgtt gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga
     7261 ttatggtagg cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac
     7321 tgaaaaaaga ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca
     7381 gtttcctgaa aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt
     7441 cggtattctc attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg
     7501 cgttgattat tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc
     7561 cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt
     7621 tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga
     7681 gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga
     7741 tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat
     7801 ctgctttggt cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat
     7861 cacaataata tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg
     7921 ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt
     7981 ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag
     8041 cgatttaaga tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta
     8101 agccgcgcct gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc
     8161 caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga
     8221 cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga
     8281 gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt
     8341 catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt
     8401 gtcaggcacg cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc
     8461 agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc
     8521 tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg
     8581 tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc
     8641 gtattgagag ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg
     8701 atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc
     8761 atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc
     8821 aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg
     8881 cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg
     8941 cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact
     9001 gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg
     9061 cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac
     9121 gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg
     9181 aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc
     9241 ttctcttaca agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg
     9301 aactccgcac cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg
     9361 cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg
     9421 ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt
     9481 cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg
     9541 ttatcccctt acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg
     9601 tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag
     9661 tcttacatga ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt
     9721 gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg
     9781 gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa
     9841 gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg
     9901 gagccggaag gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg
     9961 ctggagcagg cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta
    10021 acctgtctgg aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg
    10081 aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc
    10141 gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa
    10201 gatctgcatc cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat
    10261 ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt
    10321 cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc
    10381 acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat
    10441 agctattttg cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg
    10501 cgtacccgta gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag
    10561 ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg
    10621 ttgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag
    10681 cgccgtctgc gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt
    10741 attcgctatg atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc
    10801 aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg
    10861 ccgcagtata acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca
    10921 tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg
    10981 gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg
    11041 caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg
    11101 gtaaacctgg ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg
    11161 gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg
    11221 gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac
    11281 gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt
    11341 tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa
    11401 tacccatccg cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg
    11461 acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca
    11521 ccggataaat taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg
    11581 ccagtggatg gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat
    11641 gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca
    11701 acgcgggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt
    11761 tcttatggaa attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg
    11821 caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg
    11881 gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct
    11941 ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag
    12001 acagggcgga ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc
    12061 gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca
    12121 tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg
    12181 gacggctaaa atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg
    12241 gtcacttact tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa
    12301 atccatagga tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg
    12361 gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg
    12421 ggaactccac gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg
    12481 atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc
    12541 atgtactgac caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg
    12601 gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac
    12661 ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg
    12721 agttcgttaa aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg
    12781 aggcgctggc gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt
    12841 ggctggggaa agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc
    12901 cggtttatca gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg
    12961 atgaacccgc gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt
    13021 acgacgcgct ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc
    13081 caaatctcga cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg
    13141 gtaaagataa cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg
    13201 gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta
    13261 aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc
    13321 ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg
    13381 ccctacaaaa atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg
    13441 cagctctggc agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata
    13501 atccggcggt agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg
    13561 tctatgaagt gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca
    13621 ccacgattgg ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa
    13681 agggcaatct cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca
    13741 ttgttgagca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg
    13801 acatggtgga atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg
    13861 tacagagcta cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc
    13921 cggcaccgat taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga
    13981 aagggatgct gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca
    14041 gccgtgaaac catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg
    14101 aagccgctgg aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc
    14161 tgcgtcgtag cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg
    14221 ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg
    14281 acatcatgga ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt
    14341 ccgacatgga gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg
    14401 gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga
    14461 agaaagcggc aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga
    14521 aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga
    14581 atttgcgtcg ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca
    14641 gcgacttctt cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg
    14701 cagattcggc atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt
    14761 acacgataat ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc
    14821 tctcctgcgg aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat
    14881 cggttgcgat atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc
    14941 cgtaccacgc cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac
    15001 gtccacccca gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag
    15061 cccaactggc gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca
    15121 gaccgctaag caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa
    15181 gttcaggtgc gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat
    15241 gttcatgcac gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt
    15301 gctttggtct ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc
    15361 ccgccacaat cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag
    15421 gtgcaaatcc agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt
    15481 gcgcaatgtt aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt
    15541 tatgcagtcc tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa
    15601 tttatgcaac gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg
    15661 taaagtgtct ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa
    15721 gtctgatgtt tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat
    15781 cgtccctggc gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa
    15841 gctggcatct caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat
    15901 cgtctgctct accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca
    15961 gctgggcatt cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa
    16021 tgtgggtgat gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt
    16081 cgcaccgctg gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc
    16141 tgcgaaatct attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta
    16201 cccaggtcag gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc
    16261 tatggaagag tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct
    16321 gaccatgtgt gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg
    16381 cacccagcaa gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa
    16441 aatcgtggtg gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac
    16501 gcgttcggcc ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg
    16561 tttgtacagc acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat
    16621 ttctcaatca tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt
    16681 gccagttatc aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc
    16741 gcggagttaa gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt
    16801 gaattgctca ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat
    16861 ctgcgtgaag taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc
    16921 cagatgatta acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt
    16981 tttgagcaca gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg
    17041 tcgccgctac gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa
    17101 gaagcacaag aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg
    17161 caaatggccc aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag
    17221 ggcaactggg gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg
    17281 tatgaatatg aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat
    17341 gtcatcgtgc ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc
    17401 gcctatgaac gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa
    17461 catatcgcgt cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg
    17521 ccggggctgc gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta
    17581 ctggcgcttg accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg
    17641 gttagcccga ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag
    17701 catcaaagcc gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg
    17761 cgtttgttca tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac
    17821 taccgccagg caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa
    17881 gcgtttcgcg gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag
    17941 gcggtgagcc aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa
    18001 gcttatcaac gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa
    18061 gccgggtaga aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg
    18121 atgagcaggc attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac
    18181 cgtcgcgaag gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa
    18241 atacgatgtc atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac
    18301 gattgattgc agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg
    18361 cgacctgaca gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga
    18421 tatcaatgaa tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg
    18481 caacgttgag tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga
    18541 ttgcatcacc atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc
    18601 aatgtatcgc gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat
    18661 tatcgagccg ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg
    18721 ctcactggtc gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca
    18781 tcccgatcag gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta
    18841 ctacaatctg acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac
    18901 cggaaatgcc ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc
    18961 tgtatcgctc acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg
    19021 tggaggtaaa aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg
    19081 tactgggcga atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc
    19141 cgaaacttcg cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc
    19201 agggcgatat tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc
    19261 ctgcggaact gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca
    19321 tgcgcggagg cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg
    19381 aagccattac tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg
    19441 aagagacggc tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg
    19501 aggctaaatg acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag
    19561 ctacggactt gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg
    19621 atactcatta ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg
    19681 actggccctg caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg
    19741 ccgcgatctt tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc
    19801 tccgtttgat ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt
    19861 agaagcgtgg tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt
    19921 tcataccgcg cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga
    19981 tattttgccg gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc
    20041 gcgtttgctg ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa
    20101 gaccttgatt gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg
    20161 caattttgaa gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga
    20221 agggatgatg gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga
    20281 gaaaaacggc actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca
    20341 ggtctttcgc gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta
    20401 tgaacacccg gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa
    20461 caaagaagat aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg
    20521 caaagtggca gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga
    20581 gttcgaattt gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat
    20641 ttcgtttgga catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt
    20701 gcagccgcaa ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca
    20761 gctttatccg cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa
    20821 agatcaggtc ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt
    20881 cgaaaaaatg ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt
    20941 acagcacagt gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca
    21001 atcgcgttat tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt
    21061 cagccgacct gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg
    21121 gtttgtcggt tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg
    21181 tataatgcgg ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag
    21241 tatttggcag ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct
    21301 cggctccatc ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga
    21361 tgatgaacca aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc
    21421 cgataagcag gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga
    21481 taaagagcag gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat
    21541 catcggcctc gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg
    21601 ctggattcgc gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt
    21661 aaaactccag gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct
    21721 gacgcccgaa ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg
    21781 ttcctacgtt gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc
    21841 ggtggtgaaa gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca
    21901 ggccagttcg ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc
    21961 ggacgctgaa ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg
    22021 tctgtagaag atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg
    22081 aactgcatta tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc
    22141 tatcacttgg tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc
    22201 accgacgtgg cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt
    22261 ctgtcagcgc cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag
    22321 catgaacgtc gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc
    22381 atggcattcg cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg
    22441 ccggaagggg tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg
    22501 tttatggcgt ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg
    22561 gggattacct cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc
    22621 gttgtcggga tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg
    22681 atgtactgtc tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga
    22741 aatcgggaag aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa
    22801 ccgcccgtca gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga
    22861 ccagttcgca atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag
    22921 ttaatgggct actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg
    22981 cgcgtcagta ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt
    23041 ggcaagctgc gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga
    23101 ttggtgaatg tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg
    23161 cttttgttgc tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc
    23221 gcgatgccca cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg
    23281 cggttcttca ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa
    23341 tttatatcgg cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat
    23401 tgttgccgtt gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc
    23461 cgcgcgatct cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata
    23521 ttttgcaacg tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg
    23581 atgccaatgt caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct
    23641 tcacactgtg cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac
    23701 catcgggttc ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct
    23761 tatcacccgg ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct
    23821 gatgaattac cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga
    23881 cgccacgcgt agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca
    23941 ggtagttagg gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca
    24001 gggtgatcat cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt
    24061 gaagttgccc gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt
    24121 taatcggggc gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc
    24181 gttataccgt cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc
    24241 gtataatggg cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg
    24301 acttcctgac gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc
    24361 cgcatctgga aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt
    24421 tgttcgaaaa ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa
    24481 agcgcgtggc gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat
    24541 tattggcgtt tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac
    24601 tgccgcagtt taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc
    24661 aacaaaaaat cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct
    24721 ggccggaaga tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata
    24781 aagagcggca gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta
    24841 tgcgctggct gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc
    24901 cgggcgaaca tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg
    24961 cagtcacccc cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca
    25021 ccaaaaccga agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa
    25081 tcgtcctgga agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc
    25141 acacaggtta ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc
    25201 agcgtgaaga tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg
    25261 tactgggcgt ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa
    25321 ttgtcgattt ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca
    25381 aaaaacagta cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc
    25441 agtttatgta cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga
    25501 acgatgtgat ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag
    25561 aaaatacgcc tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa
    25621 tggggctgga tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca
    25681 tcaaaaaaga tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt
    25741 ttaacaacgg taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga
    25801 gaaagcgcat gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg
    25861 tatatcgtgt ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga
    25921 tggtagtgat ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa
    25981 aagggtttat cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca
    26041 tggaccgcat cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc
    26101 tgcgcgatga tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg
    26161 cccgctcgat tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact
    26221 ggggcgggcg tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa
    26281 agcaccttgg tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc
    26341 gtactggtac cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata
    26401 tctatattgc cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc
    26461 gtaatgcgcg ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa
    26521 aacccgcccc tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat
    26581 ctatgtcgca tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag
    26641 gcctacattg gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg
    26701 gttaaacccg ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca
    26761 gaccaaactg aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac
    26821 cggaacaacc cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt
    26881 caatctgctc cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt
    26941 cgttcatctc aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct
    27001 tcgaggccgg aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg
    27061 aacgcacacg agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca
    27121 gcatggcagc tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta
    27181 ctggatcaaa cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca
    27241 cttcgtcgta attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga
    27301 tttcattttt aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg
    27361 caaaggcatc ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta
    27421 agcccatcat gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc
    27481 cgtgactcat cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat
    27541 cgccagtcat gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc
    27601 gattgacggt aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg
    27661 cgatgttaaa accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg
    27721 ccgccgcttc cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat
    27781 cttccgcacg cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg
    27841 catcgacaat gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg
    27901 gacgggctgg ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac
    27961 cttccggcac ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt
    28021 ttgcgctacc gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca
    28081 ggccgtagac cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac
    28141 agcgcaccac ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga
    28201 aatcgcgctt cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt
    28261 tcttcggctt acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac
    28321 caaagcggtt ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct
    28381 gcgggaagcc tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt
    28441 ccagcagata tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct
    28501 tgcggaaatc cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca
    28561 cgcggttaac aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc
    28621 tcgcccaggc gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt
    28681 ctaccaacgg cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc
    28741 gttccagcgc gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg
    28801 tatcctggcg tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt
    28861 caacaaccgc ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt
    28921 ggatcgtgga gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct
    28981 tgttcagcag tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt
    29041 tcatgacaac cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc
    29101 ctgcgcccag cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt
    29161 tgccttttac atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat
    29221 gcgccagcgg gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg
    29281 ccgcagcttc aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt
    29341 gtgcgaccat tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta
    29401 gcggttcctg cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc
    29461 gtaaaatcgc catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac
    29521 cgattttcag cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac
    29581 tgtcagcgcc cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca
    29641 gtttggtttc cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca
    29701 gcacgcattc gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg
    29761 gcagatcttc caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt
    29821 cttcaggaac gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg
    29881 ctttgttcga acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg
    29941 cctcgccgag gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa
    30001 ataccagttc ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa
    30061 gcatgtcagt ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt
    30121 gttttgtgtt aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg
    30181 cagaaatcac gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac
    30241 agaagggtaa aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca
    30301 agaacggact cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg
    30361 cgagctgttc aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt
    30421 caaagcgtgg gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa
    30481 caagccgaaa ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc
    30541 gacctccttc tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg
    30601 tagcctgctg cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc
    30661 gctgcaactg ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca
    30721 ttattaccgc tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat
    30781 ggcggtcaat ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga
    30841 tatcaatatt gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa
    30901 cattgtggcg cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc
    30961 accggaagag atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc
    31021 tgacctgacc cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga
    31081 cgtaaatgat gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga
    31141 ttaccacatc cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac
    31201 cgatatgagt gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg
    31261 tattggccat ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag
    31321 cggtacacac ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca
    31381 gccgggcatg gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc
    31441 gtggcgtgaa gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc
    31501 gttcggcggc attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat
    31561 gacccgggat ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg
    31621 gtcgttgaag agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt
    31681 gaggcggcga aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat
    31741 tgcgtggcgt gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac
    31801 ggggagccgg cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc
    31861 ggggaaatta ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg
    31921 ttagtgaaag cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc
    31981 aagacgccat taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa
    32041 gcgttgctgg ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg
    32101 ctgcgggtgg cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt
    32161 agccgtggtt cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa
    32221 ctaaggaagc ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca
    32281 tcttattttc agggaccatg attatccctg ggctggtagc actcatctac cgggatggag
    32341 cgggccgcgc ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt
    32401 ggccgaaccg caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc
    32461 tgttttggac cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga
    32521 acctcacgat taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca
    32581 ctacgctggt ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc
    32641 aatggtttgg cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg
    32701 tgggtgggat gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc
    32761 gcccgcgaat tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg
    32821 cctgtgcgct ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct
    32881 ttgcgactat cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca
    32941 gtccgactat taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc
    33001 tgcacttttc actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc
    33061 gcatgtttat cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc
    33121 ataatgtcta cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga
    33181 tggcgacaac cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg
    33241 tactgctttt atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga
    33301 aagtgatccg catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc
    33361 atccgaacgc cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg
    33421 aagccgtttg gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga
    33481 ttatcgccac gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata
    33541 acctggggcc agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta
    33601 aatggatcct gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc
    33661 tctttacccc gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt
    33721 ctcaacaagg gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga
    33781 actggggatc cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa
    33841 ctatgaccgt gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca
    33901 ggaatttgtc aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt
    33961 aaacctggtg gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa
    34021 gtttctgatg aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg
    34081 ttacccacgt tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg
    34141 cggtgaaacg gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt
    34201 cgcccgagaa atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa
    34261 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
    34321 aatgcgccac cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc
    34381 tgagaattcc gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt
    34441 atgaacaccc tgcgc
//
LOCUS       DAJTUN010000031        33740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTUN010000031 DAJTUN010000000
VERSION     DAJTUN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.031"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..568
                     /locus_tag="OO470_001418"
     CDS             <1..568
                     /locus_tag="OO470_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6250717.1"
                     /translation="AFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRY
                     LEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSA
                     LELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRVQYV
                     LDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(565..1701)
                     /locus_tag="OO470_001419"
     CDS             complement(565..1701)
                     /locus_tag="OO470_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6250718.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1707..2030)
                     /locus_tag="OO470_001420"
     CDS             complement(1707..2030)
                     /locus_tag="OO470_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6250719.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2213..2356
                     /locus_tag="OO470_001421"
     CDS             2213..2356
                     /locus_tag="OO470_001421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250720.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2575..4113)
                     /gene="aldB"
                     /locus_tag="OO470_001422"
     CDS             complement(2575..4113)
                     /gene="aldB"
                     /locus_tag="OO470_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6250721.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4278..5429)
                     /gene="yiaY"
                     /locus_tag="OO470_001423"
     CDS             complement(4278..5429)
                     /gene="yiaY"
                     /locus_tag="OO470_001423"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6250722.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5619..7463)
                     /gene="selB"
                     /locus_tag="OO470_001424"
     CDS             complement(5619..7463)
                     /gene="selB"
                     /locus_tag="OO470_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6250723.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGMALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7460..8851)
                     /gene="selA"
                     /locus_tag="OO470_001425"
     CDS             complement(7460..8851)
                     /gene="selA"
                     /locus_tag="OO470_001425"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6250724.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8949..9557)
                     /locus_tag="OO470_001426"
     CDS             complement(8949..9557)
                     /locus_tag="OO470_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6250725.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9628..10713)
                     /locus_tag="OO470_001427"
     CDS             complement(9628..10713)
                     /locus_tag="OO470_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6250726.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10716..11078)
                     /locus_tag="OO470_001428"
     CDS             complement(10716..11078)
                     /locus_tag="OO470_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6250727.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11615..13528
                     /gene="mtlA"
                     /locus_tag="OO470_001429"
     CDS             11615..13528
                     /gene="mtlA"
                     /locus_tag="OO470_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6250728.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13623..14771
                     /gene="mtlD"
                     /locus_tag="OO470_001430"
     CDS             13623..14771
                     /gene="mtlD"
                     /locus_tag="OO470_001430"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6250729.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14771..15358
                     /gene="mtlR"
                     /locus_tag="OO470_001431"
     CDS             14771..15358
                     /gene="mtlR"
                     /locus_tag="OO470_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6250730.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15370..15579)
                     /locus_tag="OO470_001432"
     CDS             complement(15370..15579)
                     /locus_tag="OO470_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250731.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15863..16225
                     /locus_tag="OO470_001433"
     CDS             15863..16225
                     /locus_tag="OO470_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6250732.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16769..17452
                     /locus_tag="OO470_001434"
     CDS             16769..17452
                     /locus_tag="OO470_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6250733.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17496..21861
                     /locus_tag="OO470_001435"
                     /pseudo
     CDS             17496..21861
                     /locus_tag="OO470_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22229..23884
                     /gene="lldP"
                     /locus_tag="OO470_001436"
     CDS             22229..23884
                     /gene="lldP"
                     /locus_tag="OO470_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6250734.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23884..24660
                     /gene="lldR"
                     /locus_tag="OO470_001437"
     CDS             23884..24660
                     /gene="lldR"
                     /locus_tag="OO470_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6250735.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24657..25847
                     /gene="lldD"
                     /locus_tag="OO470_001438"
     CDS             24657..25847
                     /gene="lldD"
                     /locus_tag="OO470_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6250736.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26043..26516
                     /gene="trmL"
                     /locus_tag="OO470_001439"
     CDS             26043..26516
                     /gene="trmL"
                     /locus_tag="OO470_001439"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6250737.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26569..27390)
                     /gene="cysE"
                     /locus_tag="OO470_001440"
     CDS             complement(26569..27390)
                     /gene="cysE"
                     /locus_tag="OO470_001440"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6250738.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27470..28489)
                     /gene="gpsA"
                     /locus_tag="OO470_001441"
     CDS             complement(27470..28489)
                     /gene="gpsA"
                     /locus_tag="OO470_001441"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6250739.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28489..28956)
                     /gene="secB"
                     /locus_tag="OO470_001442"
     CDS             complement(28489..28956)
                     /gene="secB"
                     /locus_tag="OO470_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6250740.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29019..29270)
                     /gene="grxC"
                     /locus_tag="OO470_001443"
     CDS             complement(29019..29270)
                     /gene="grxC"
                     /locus_tag="OO470_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6250741.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29412..29843)
                     /locus_tag="OO470_001444"
     CDS             complement(29412..29843)
                     /locus_tag="OO470_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6250742.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30088..31632
                     /gene="gpmM"
                     /locus_tag="OO470_001445"
                     /gene_synonym="pgmI"
     CDS             30088..31632
                     /gene="gpmM"
                     /locus_tag="OO470_001445"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6250743.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31642..32925
                     /gene="envC"
                     /locus_tag="OO470_001446"
     CDS             31642..32925
                     /gene="envC"
                     /locus_tag="OO470_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6250744.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32929..>33740
                     /locus_tag="OO470_001447"
     CDS             32929..>33740
                     /locus_tag="OO470_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6250745.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPK"
BASE COUNT         8378 a         8842 c         8601 g         7919 t
ORIGIN      
        1 tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca gctacgctcc
       61 accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa ccgcttatgc
      121 cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg aacatcactc
      181 ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct tcaacgtcgc
      241 cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg ataacacgca
      301 atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag acaaaagtgt
      361 gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt atgaatggtg
      421 gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg tccagtatgt
      481 gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc aactgcggcc
      541 tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca cccagctggt
      601 catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag agtagaccgc
      661 cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat ccagttcaat
      721 ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg tgccggtcga
      781 ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc tgaatacctt
      841 acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc gcagcaagga
      901 gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca ccatcaccgg
      961 acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg tcacataacc
     1021 atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg cgagctgcgc
     1081 tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct ggctctggat
     1141 ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat aattttgccg
     1201 cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc cgcgtgcgta
     1261 acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag tattcgccgc
     1321 catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa cgatatccgc
     1381 catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata gcacctcacc
     1441 ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg tcacctgtgg
     1501 gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat ggttatagtt
     1561 cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg tggaaatggt
     1621 ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag ccacataggt
     1681 caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat gtcggcgagc
     1741 tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc atggatttgc
     1801 attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa tggccagata
     1861 acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc atgaggatga
     1921 ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc atagaatatc
     1981 accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat atttccctca
     2041 aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca gtgcaatttc
     2101 cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct gtctggtttt
     2161 tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac taatgaataa
     2221 ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta tctaccgttt
     2281 attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc cgctcaccat
     2341 cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc tgttggcgta
     2401 gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc gctttgttta
     2461 tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta ggcatgataa
     2521 gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct tatatcagaa
     2581 cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct ggtaatgctc
     2641 cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac caaacgcagc
     2701 atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta tgccgcgccc
     2761 catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc catattgcgt
     2821 atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg ccagcaccgg
     2881 gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa tcgtcggttc
     2941 gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc ctgtgagcac
     3001 gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca gttgcccgtg
     3061 agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc gaatgctttc
     3121 gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca ctaaggcacg
     3181 actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc cttccagcgc
     3241 tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat ttggcgactt
     3301 accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca taatttgttg
     3361 gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg tcgccagata
     3421 ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg gcagtaaatc
     3481 accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg gtttcagcac
     3541 cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca gcagcgggaa
     3601 gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat aggccacggt
     3661 ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg cgaaatagcg
     3721 gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg gtttgccgtt
     3781 atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat cggcaatctt
     3841 aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt tcactttgtg
     3901 cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt cgcacagcag
     3961 ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg ctacccattc
     4021 accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat actcgccggg
     4081 cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt gtgaggtata
     4141 acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta cgacagctat
     4201 cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca atttgccagc
     4261 cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc ttcgtgagtt
     4321 gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc cagaaccgcg
     4381 aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac tttcttcgcc
     4441 agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt acctgtcacg
     4501 ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt gctgttgaat
     4561 acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt gtagaaaccg
     4621 cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa cgccataccg
     4681 gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc atcttcaacg
     4741 gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc gtcagtgatc
     4801 ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc catacccgtt
     4861 gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac agaaagcagc
     4921 ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc agtgatgatg
     4981 cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat catcggcagc
     5041 tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc attggctgcc
     5101 accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga gatcacgcta
     5161 tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt ggtggggtta
     5221 ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc tttttgcaca
     5281 tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt acgggtaaat
     5341 ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat gacattcaca
     5401 gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa aaaagggtgg
     5461 gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta tcaaaattaa
     5521 tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat cataagaaat
     5581 ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg aaataataat
     5641 gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg gtcaaaatat
     5701 tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa atccgccgcg
     5761 caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc gacaatccga
     5821 tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg cgccgcctgg
     5881 cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag atcacgcacc
     5941 caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg ctgctcttcg
     6001 ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag gatgtcgccg
     6061 ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat tggcaacgcc
     6121 atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc atgataagtc
     6181 gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt caacaagcta
     6241 taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc gccgttgagc
     6301 tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg aacggctaac
     6361 gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata ctctggttta
     6421 cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag cgtgttgcgg
     6481 gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg ggtgtcgaag
     6541 accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg gctggcggcg
     6601 tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag ctcgacaatc
     6661 acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg gttaatctgc
     6721 tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt ggcggtttct
     6781 gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac accagtcagc
     6841 cagagtgaat cgcctacctt cacttccccg cttaacgcca taccggtgac gaccagcccg
     6901 gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg gctggcgtgc
     6961 tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg accttcggtt
     7021 gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac ctcctttacc
     7081 tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt cagcgccact
     7141 gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt ctgtgccatc
     7201 acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac gcccgccagc
     7261 atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac gcgaccatcc
     7321 ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt ttcttccggc
     7381 agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc gtggtcaacg
     7441 tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc tcatcttcaa
     7501 ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc ggcactggca
     7561 attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca tggggtgtaa
     7621 acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca atctgcgaaa
     7681 gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg ggagcctgta
     7741 aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc agggtcggta
     7801 atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc agcgccgcga
     7861 gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg cgggcgatca
     7921 tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg tcgccggaga
     7981 agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct ttcggcaaac
     8041 cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact ggaacatcca
     8101 gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac ccctgaatgc
     8161 tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc gcctgacgat
     8221 aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag cctgcctgac
     8281 gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg cgagatacca
     8341 ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc gcattgttat
     8401 tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc agcgcccgat
     8461 cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc gaacgcatag
     8521 cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg ttggtatgca
     8581 gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct ttcgtcaacc
     8641 gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg ctgccacgaa
     8701 tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg gtgtgaccat
     8761 aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata gccggaagtt
     8821 gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa ggccctctca
     8881 cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc aggaggacat
     8941 atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct aaacaggttt
     9001 tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac gcggcggaaa
     9061 ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt atcggttttg
     9121 agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat tttctcccgc
     9181 tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc acgcaccgat
     9241 accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac ccgcaacgac
     9301 tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat atattcagcg
     9361 atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg cactttccct
     9421 aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt aataaattcg
     9481 aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg gctggtgtag
     9541 ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta tggctcaggc
     9601 tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat gcatccaact
     9661 ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt cggagtaaac
     9721 cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag ggtccagttc
     9781 aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca ataccccctg
     9841 cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc cgtggaacac
     9901 ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca gacgtaacag
     9961 cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga agaccatcac
    10021 cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct gagtgacgta
    10081 gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt cagtaagttg
    10141 cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga gctgactctg
    10201 gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga ggaaattttg
    10261 ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc tgcctttcag
    10321 gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg tggtgttggc
    10381 ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg tcatcaggtc
    10441 agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa aaagcacctc
    10501 gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc ccgtcacctg
    10561 tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta
    10621 gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg ccgtcgccag
    10681 cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa cgtggaatag
    10741 tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc agaaagtgcg
    10801 gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca
    10861 cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta
    10921 aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg
    10981 gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg
    11041 aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta
    11101 tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc
    11161 cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta
    11221 gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc
    11281 gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg
    11341 tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta
    11401 tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga
    11461 ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca
    11521 gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc
    11581 ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt
    11641 gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc
    11701 gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc
    11761 gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg
    11821 taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca tgggcgttat
    11881 cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg
    11941 gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct
    12001 ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg
    12061 cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt
    12121 tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct
    12181 caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact
    12241 gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct
    12301 ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat
    12361 ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct
    12421 gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg
    12481 tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa
    12541 aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt
    12601 ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac
    12661 tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcga
    12721 tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg
    12781 ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca
    12841 gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac
    12901 tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct
    12961 gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca
    13021 gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct ttgacgattc
    13081 cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca aagcagcaac
    13141 caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt acgttgagcc
    13201 ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc tgggtgagtc
    13261 tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa cgggcgtggt
    13321 gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca ttgcccgtct
    13381 ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca gcctgaccaa
    13441 tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg tggatgaagt
    13501 gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac ggagagggtt
    13561 tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg aaggttaata
    13621 ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc ggtaaactgc
    13681 tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta cttgatgccc
    13741 tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag gtagataccg
    13801 tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg attgctcagg
    13861 tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct ccggctatcg
    13921 ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac atcatcgcct
    13981 gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac gccctgccgg
    14041 aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc gttgaccgca
    14101 tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta gaaactttca
    14161 gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc ccaggcatgg
    14221 agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg aacacgggtc
    14281 atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt gacgctattc
    14341 tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt gcggtactga
    14401 tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa atcctcggtc
    14461 gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag ccgctgcgta
    14521 aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa tacagccttc
    14581 cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt gaagatgatc
    14641 cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg gcgctggcac
    14701 agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc gcttataaag
    14761 caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca gattgtcaga
    14821 tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg agcgtctgaa
    14881 tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga ccgaggcggt
    14941 aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt atgctgtaga
    15001 accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa aactcattta
    15061 cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga tggcattgcg
    15121 tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa tccttggacc
    15181 ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg aaccagcaga
    15241 ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat caacaatggt
    15301 cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc aaaagtaagc
    15361 aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat aatactccag
    15421 acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg cgttacgggg
    15481 aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat ctacggcttt
    15541 atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc cgtattaccc
    15601 cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga tgacattaag
    15661 gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt tctatagatt
    15721 tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg ttgcagaact
    15781 attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg gctataaatt
    15841 tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca agcgtctcag
    15901 cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg aagccgcaga
    15961 caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg ctcgcctgcg
    16021 tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga tgccgttcca
    16081 gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg
    16141 actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg aagagatgac
    16201 tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg tatcaacccg
    16261 catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc ctaattacca
    16321 aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa tgcattttta
    16381 ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt taattaacaa
    16441 gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat attcacaaaa
    16501 tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca tttattaata
    16561 atcatcatta gaatccacga aaaataatat attcatacaa acacagacat cttttgaaac
    16621 gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca catcactgtc
    16681 attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc ggcattgatc
    16741 gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt tcagtgctgt
    16801 cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag gcaagccaga
    16861 aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac ttgaaaaaca
    16921 acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg gcataataaa
    16981 cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa atacttttat
    17041 ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg gttcggtggc
    17101 tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta ttcatatctc
    17161 actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga catggggtgg
    17221 tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc
    17281 aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa
    17341 gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca
    17401 gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat aaatgcagtt
    17461 agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt aaagttatct
    17521 ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga
    17581 aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt
    17641 ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac ggtagtggat
    17701 cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact tttatgaaca
    17761 ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa tatagctctg
    17821 ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa
    17881 tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg ggccaatact
    17941 caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct atgggggact
    18001 cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta gctagcggta
    18061 ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct attgcgatag
    18121 gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag
    18181 ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat actatcgcat
    18241 taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag ggcaacaaag
    18301 ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc ttaaacacca
    18361 ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt ggtagtaata
    18421 gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg gatttagaca
    18481 ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg aaaaatggcg
    18541 cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc attagcgact
    18601 cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg tactgttaca
    18661 gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc gctcgctgta
    18721 cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc tgctcatggt
    18781 actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat ttcagcctcc
    18841 agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt cgaagccaac
    18901 accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga tgccctgctc
    18961 tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac cagcaaaatc
    19021 accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa cggctctcag
    19081 ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa taacacttcc
    19141 aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac taatcttggt
    19201 gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca tggcaccgag
    19261 accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag caccgatgcc
    19321 gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac caccaatatc
    19381 gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct tggtgaggat
    19441 gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa caataccgcc
    19501 agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga tgccattaac
    19561 ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg caatgcttct
    19621 gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac aaattattat
    19681 aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct cggcgatgct
    19741 ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa tggtgacgca
    19801 agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga cgcagtaaac
    19861 ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg tggtgccgaa
    19921 gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc cgagtacaac
    19981 aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga taagactgcc
    20041 aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat catcactaat
    20101 gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc tcagttgaat
    20161 gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc tggtaacacc
    20221 gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac taacgacaac
    20281 gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt aggttataac
    20341 gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag caccgttgat
    20401 accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa aggttctcgt
    20461 gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga cggcgaactg
    20521 ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa cgtagctgat
    20581 ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat tggagcggtc
    20641 gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc actggccgtt
    20701 ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc aggtattggt
    20761 atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat cggcagtaac
    20821 gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac cacccgtggt
    20881 gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt aggtgagttt
    20941 tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg ttcagcggat
    21001 accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc gcaaaatacc
    21061 cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt tactaatatc
    21121 gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa gaccaacacc
    21181 gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc tggttccatt
    21241 gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga agaaaataca
    21301 atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc cagtgttaat
    21361 gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg agtgcgctac
    21421 gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg tggcaacggc
    21481 agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc ggtgaactac
    21541 gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg gatggttgag
    21601 atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg
    21661 gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc tattggtggc
    21721 ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt
    21781 cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc
    21841 ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa
    21901 ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac aatctaaggg
    21961 caattctctg atgaggattg cccttttctt taccagacat ctccccccac aagaattggc
    22021 cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc
    22081 aatattgcca atacataaca tttagttaac cattcattgt cattatccct acacaacaca
    22141 attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac
    22201 gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact acgatcccgc
    22261 cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt tcttctttgc
    22321 gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct
    22381 tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cagtggttta
    22441 tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta
    22501 taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac
    22561 ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg
    22621 agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt
    22681 taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg
    22741 cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca gctttgagat
    22801 tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt tctggatcat
    22861 ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg tggttgcagg
    22921 cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg agctgccgga
    22981 cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcaacc
    23041 agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga cgctggccca
    23101 caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgaccgc
    23161 taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct
    23221 gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg cccgtatgcc
    23281 accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc
    23341 caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc
    23401 gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac ccatctactc
    23461 catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt catcaacact
    23521 ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg
    23581 gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca
    23641 agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac
    23701 cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt
    23761 aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt
    23821 cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc
    23881 ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg
    23941 attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg
    24001 caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt gagcgaaggc
    24061 gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg
    24121 gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga ttacagtttc
    24181 gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc
    24241 gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac
    24301 ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat
    24361 aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag
    24421 catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat
    24481 caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg
    24541 gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac
    24601 gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga
    24661 ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt
    24721 tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt
    24781 tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta agcctggaaa
    24841 cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg
    24901 gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg catggcattc
    24961 cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc
    25021 gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg
    25081 agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg
    25141 gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca atgcgccgct
    25201 acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac
    25261 atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa gattacatcg
    25321 gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg
    25381 atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg
    25441 cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc cagctggacg
    25501 gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg
    25561 ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg
    25621 gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg
    25681 cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga
    25741 ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg ctgggtaaag
    25801 agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg tttgcgcccc
    25861 ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg tactccctca
    25921 ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg gccggggtga
    25981 ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac taagggggca
    26041 gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc aacatcatcc
    26101 gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga tttgcctggg
    26161 acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc
    26221 atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca
    26281 cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat ctgatgttcg
    26341 gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa
    26401 ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg gtgtcggtag
    26461 tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga gattagttac
    26521 tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt agatcccatc
    26581 cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt
    26641 atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg tatgcggcgg
    26701 caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt
    26761 gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc
    26821 accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc
    26881 aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc
    26941 aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa
    27001 gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa
    27061 ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg
    27121 ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta
    27181 ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt
    27241 cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg tcgcgtggta
    27301 aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt
    27361 ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg
    27421 gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtgactgc
    27481 tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc gcgttttttc
    27541 cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg aagcgatgcg
    27601 ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct
    27661 gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg ttacgcgact
    27721 ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata aaggtggcag
    27781 gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg gtgatcagcg
    27841 ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca atggcaatga
    27901 cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg taaacgcgga
    27961 aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga tcggtcgagg
    28021 ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga
    28081 taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc
    28141 cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg
    28201 gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac
    28261 gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca
    28321 catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt
    28381 catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca agagcggtgc
    28441 cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg
    28501 atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa
    28561 gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc tggtgatgca
    28621 ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg
    28681 ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc
    28741 ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc
    28801 tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg
    28861 gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat
    28921 ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt
    28981 ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag gggatccagt
    29041 ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata
    29101 aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg cttggcggca
    29161 ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag tgcttttgca
    29221 cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat aatttagctc
    29281 ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag tgtccccaga
    29341 agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga
    29401 cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca gccagcaacg
    29461 ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc
    29521 tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg cttctcaagc
    29581 tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc tgcgatatgg
    29641 cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc
    29701 agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta
    29761 gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca
    29821 acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac
    29881 caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca
    29941 attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc
    30001 gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt
    30061 ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg
    30121 attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa
    30181 accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt
    30241 ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg
    30301 ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc
    30361 gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa cgcaggtaaa
    30421 gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga agatcacatc
    30481 atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc
    30541 cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag
    30601 aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg
    30661 gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag
    30721 ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa
    30781 aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg
    30841 gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact
    30901 cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat
    30961 ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct
    31021 tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt
    31081 tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg
    31141 ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa
    31201 ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa
    31261 tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg
    31321 gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg
    31381 gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg
    31441 cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt
    31501 tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga catcgcgccg
    31561 accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc
    31621 atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg
    31681 aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta
    31741 ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc
    31801 gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc
    31861 gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg
    31921 caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa
    31981 ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga tgccgcattc
    32041 cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag ccagcgtgga
    32101 cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag
    32161 ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga gaaacaaagc
    32221 gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac tcaggctctg
    32281 aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg tcagcagcag
    32341 ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg tgcggaagcc
    32401 gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg cgagcgccag
    32461 aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa atcgctgatg
    32521 tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg
    32581 acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg gatggttatc
    32641 ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat tctggctgac
    32701 tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga catgagtctt
    32761 tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc gggccagcca
    32821 attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt cgaaattcgc
    32881 cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt gtttccattt
    32941 cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt acttgctggc
    33001 aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg
    33061 gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg
    33121 gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat ggcaccgttg
    33181 agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga cgaaattgag
    33241 cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa caaccacatg
    33301 ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc gctggagcgt
    33361 tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg
    33421 caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc gcagaacgaa
    33481 gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa cggttcaacc
    33541 attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat ggtttataac
    33601 ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat
    33661 acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt ccgtggcgtg
    33721 aagctgtgca aaccgaagaa
//
LOCUS       DAJTUN010000032        33284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTUN010000032 DAJTUN010000000
VERSION     DAJTUN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.032"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            65..1342
                     /gene="waaA"
                     /locus_tag="OO470_001448"
     CDS             65..1342
                     /gene="waaA"
                     /locus_tag="OO470_001448"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6250746.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1350..1829
                     /gene="coaD"
                     /locus_tag="OO470_001449"
     CDS             1350..1829
                     /gene="coaD"
                     /locus_tag="OO470_001449"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6250747.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1868..2677)
                     /gene="mutM"
                     /locus_tag="OO470_001450"
     CDS             complement(1868..2677)
                     /gene="mutM"
                     /locus_tag="OO470_001450"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6250748.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2775..2942)
                     /gene="rpmG"
                     /locus_tag="OO470_001451"
     CDS             complement(2775..2942)
                     /gene="rpmG"
                     /locus_tag="OO470_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6250749.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2963..3199)
                     /gene="rpmB"
                     /locus_tag="OO470_001452"
     CDS             complement(2963..3199)
                     /gene="rpmB"
                     /locus_tag="OO470_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6250750.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3416..4084)
                     /gene="radC"
                     /locus_tag="OO470_001453"
     CDS             complement(3416..4084)
                     /gene="radC"
                     /locus_tag="OO470_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6250751.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4256..5476
                     /gene="coaBC"
                     /locus_tag="OO470_001454"
     CDS             4256..5476
                     /gene="coaBC"
                     /locus_tag="OO470_001454"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6250752.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5454..5912
                     /gene="dut"
                     /locus_tag="OO470_001455"
     CDS             5454..5912
                     /gene="dut"
                     /locus_tag="OO470_001455"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6250753.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6019..6615
                     /gene="slmA"
                     /locus_tag="OO470_001456"
     CDS             6019..6615
                     /gene="slmA"
                     /locus_tag="OO470_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6250754.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6652..7293)
                     /gene="pyrE"
                     /locus_tag="OO470_001457"
     CDS             complement(6652..7293)
                     /gene="pyrE"
                     /locus_tag="OO470_001457"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6250755.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7359..8075)
                     /gene="rph"
                     /locus_tag="OO470_001458"
     CDS             complement(7359..8075)
                     /gene="rph"
                     /locus_tag="OO470_001458"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6250756.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8202..9065
                     /locus_tag="OO470_001459"
     CDS             8202..9065
                     /locus_tag="OO470_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6250757.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9286..10110
                     /gene="dinD"
                     /locus_tag="OO470_001460"
     CDS             9286..10110
                     /gene="dinD"
                     /locus_tag="OO470_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6250758.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10401..11018
                     /locus_tag="OO470_001461"
     CDS             10401..11018
                     /locus_tag="OO470_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6250759.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11015..12697)
                     /gene="ligB"
                     /locus_tag="OO470_001462"
     CDS             complement(11015..12697)
                     /gene="ligB"
                     /locus_tag="OO470_001462"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6250760.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12955..13578
                     /gene="gmk"
                     /locus_tag="OO470_001463"
     CDS             12955..13578
                     /gene="gmk"
                     /locus_tag="OO470_001463"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6250761.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13633..13908
                     /gene="rpoZ"
                     /locus_tag="OO470_001464"
     CDS             13633..13908
                     /gene="rpoZ"
                     /locus_tag="OO470_001464"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6250762.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13927..16035
                     /gene="spoT"
                     /locus_tag="OO470_001465"
     CDS             13927..16035
                     /gene="spoT"
                     /locus_tag="OO470_001465"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6250763.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16042..16731
                     /gene="trmH"
                     /locus_tag="OO470_001466"
     CDS             16042..16731
                     /gene="trmH"
                     /locus_tag="OO470_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6250764.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16737..18818
                     /gene="recG"
                     /locus_tag="OO470_001467"
     CDS             16737..18818
                     /gene="recG"
                     /locus_tag="OO470_001467"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6250765.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18852..20057)
                     /gene="gltS"
                     /locus_tag="OO470_001468"
     CDS             complement(18852..20057)
                     /gene="gltS"
                     /locus_tag="OO470_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6250766.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20337..21728
                     /gene="xanP"
                     /locus_tag="OO470_001469"
     CDS             20337..21728
                     /gene="xanP"
                     /locus_tag="OO470_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6250767.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21849..23558
                     /locus_tag="OO470_001470"
     CDS             21849..23558
                     /locus_tag="OO470_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6250768.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23611..25929)
                     /gene="yicI"
                     /locus_tag="OO470_001471"
     CDS             complement(23611..25929)
                     /gene="yicI"
                     /locus_tag="OO470_001471"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6250769.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25939..27322)
                     /locus_tag="OO470_001472"
                     /pseudo
     CDS             complement(25939..27322)
                     /locus_tag="OO470_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27615..27709
                     /locus_tag="OO470_001473"
     tRNA            27615..27709
                     /locus_tag="OO470_001473"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27649..27651,aa:Sec,seq:tca)
     gene            28010..29194
                     /locus_tag="OO470_001474"
     CDS             28010..29194
                     /locus_tag="OO470_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6250770.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29606..30649
                     /locus_tag="OO470_001475"
     CDS             29606..30649
                     /locus_tag="OO470_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6250771.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31164..31592
                     /locus_tag="OO470_001476"
                     /pseudo
     CDS             31164..31592
                     /locus_tag="OO470_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31622..32773)
                     /locus_tag="OO470_001477"
                     /pseudo
     CDS             complement(31622..32773)
                     /locus_tag="OO470_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8322 a         8759 c         8216 g         7987 t
ORIGIN      
        1 gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa gtcagctatt
       61 tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc tgatctggat
      121 acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg gtgaacgtta
      181 cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg tctccgtcgg
      241 tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc ctgatttacc
      301 gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg ctttcgggaa
      361 ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc gtttcctgaa
      421 taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta acctgattgc
      481 ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct ctgcccgctc
      541 ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc gtattacgct
      601 gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg caaaaaataa
      661 ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc agttggctgc
      721 taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga ttgccaccag
      781 cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt tacagcaatt
      841 cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg atgcgattaa
      901 ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag tcccctccac
      961 cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt acggcattgc
     1021 cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc cgctggaagc
     1081 tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta aagacatttg
     1141 cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta cgcttgcaaa
     1201 agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc gtcatgccgt
     1261 tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc tggaacctta
     1321 cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat ccgggtactt
     1381 tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag atgttcgatc
     1441 acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc ctggaagagc
     1501 gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg gtcgggttta
     1561 gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt cgtggcctgc
     1621 gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc cacttaatgc
     1681 cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc tcttcatcgt
     1741 tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg gagaatgtcc
     1801 atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc catccggctc
     1861 gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca tgtttagtcg
     1921 ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa acctgcaatt
     1981 cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc gttgtaccac
     2041 cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat tcacactctg
     2101 ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac agtgattcgc
     2161 tggcatagat attccctacc cctaccacca gcttgttatc catcagccac ggtttaatcg
     2221 ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg tcgctaagcg
     2281 gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc cacagccagg
     2341 caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc accaaatcca
     2401 catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg ccagacatcc
     2461 ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta gcccgccgct
     2521 gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc ggccagcgca
     2581 agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc ggttctatgc
     2641 cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga atgaacagat
     2701 gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg tttttcgtta
     2761 catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg acgaacaact
     2821 ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt cgtagtggta
     2881 tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat acctttagcc
     2941 atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt tcagccagaa
     3001 ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg cgcagggtga
     3061 caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc aggaaacggc
     3121 gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc ttgccagtaa
     3181 cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag cttcgtatgg
     3241 ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt gaactctcga
     3301 ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata cgctgactca
     3361 gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata gcgggttaaa
     3421 tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata tggtcgagca
     3481 cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt ttatccgctt
     3541 tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc gaggcgttta
     3601 tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt agcgtgccgg
     3661 aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc accataaaga
     3721 tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc tccgggctga
     3781 gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt tcagcaatcc
     3841 cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat tgttcatatt
     3901 cagaggttaa caagccataa agagagccga aattctccag catctctttt gccagggtta
     3961 atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg acatccgtta
     4021 aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt gcattgtttt
     4081 tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt aaatcgacgg
     4141 ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg cacgacaaca
     4201 aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa tcatcatgag
     4261 cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct ataaaacccc
     4321 tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca tgaccgaagc
     4381 ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc ccgtttccga
     4441 cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg gtaaatgggc
     4501 tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg ctgccggaat
     4561 ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag ccgtgctccc
     4621 cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag aggtgcttgc
     4681 ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg gtgatatcgg
     4741 tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc atttttcgcc
     4801 cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc gtgaaccgct
     4861 cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg ctatcgccgc
     4921 cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt cactaccgac
     4981 gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag ccgccgtgaa
     5041 tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg attatcgcgc
     5101 agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg aattaacaat
     5161 aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag accatcgacc
     5221 ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc ggcaaaaacg
     5281 tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa ctcaaggatt
     5341 taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag tcttaccgct
     5401 tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc gttatgatga
     5461 aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg ctcccgactt
     5521 atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac gccgtagaac
     5581 tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt gccgatcctt
     5641 cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt atcgtgcttg
     5701 gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt tccgtgtgga
     5761 accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag atgatttttg
     5821 ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc gaccgcggtg
     5881 aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata acgtcataac
     5941 atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa gtgcttattt
     6001 tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg tcgcgaggaa
     6061 atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg tatcacgacg
     6121 gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca cttccccagt
     6181 aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat tactcgcatc
     6241 aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat tgtgttgctg
     6301 cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg tcatgcgcta
     6361 atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg tattgaagcg
     6421 cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta caccaccgat
     6481 gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc acgttttgtc
     6541 cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc gctaattgcc
     6601 gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc tttaaacgcc
     6661 aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg gcttctcttc
     6721 cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt tgtaatcacg
     6781 ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat cgagcgaaat
     6841 caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact cgcgaatcgc
     6901 cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg cgctaccaac
     6961 cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt acggcaggtc
     7021 caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga tccctttgta
     7081 agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt cagcgtaaaa
     7141 acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga agaaataggg
     7201 gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct tgctaagcgc
     7261 aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct tacttttcta
     7321 cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag cgccgccttc
     7381 tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt gagtagctct
     7441 tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg cccgtcttcg
     7501 gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc cagatcgcaa
     7561 attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt catcgggttg
     7621 gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc cacgcaggca
     7681 cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca gtccagcgta
     7741 atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg ggcgatcaga
     7801 cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg agcgttacgg
     7861 gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg gccctgacct
     7921 ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt ggtatcgcca
     7981 aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag ggtaacggga
     8041 cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc ttgaaacaaa
     8101 tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag gcatcgatgg
     8161 ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc agtatgaccg
     8221 cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa atgcgctcgg
     8281 taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt agccttgaac
     8341 ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa tgtaccctgc
     8401 gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa ctggctaaac
     8461 agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa atcaacccgg
     8521 ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc gacgcgattg
     8581 ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc gcgcgcgaaa
     8641 ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc accgccgaag
     8701 tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag cgtctggtcg
     8761 cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag gagttggttc
     8821 tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg catgttaaag
     8881 agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac tttatgatgc
     8941 aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc gaagtgacaa
     9001 actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt cagaatattg
     9061 agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc actcaaacag
     9121 agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc cagattttgc
     9181 gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat ataaatacag
     9241 ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa cgaacatcat
     9301 caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg gtcagcaaga
     9361 caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt attaacgcgc
     9421 gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga ggagatcctc
     9481 gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt actctcccgc
     9541 tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat tgcggcaggg
     9601 cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga agcatttaag
     9661 caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga acataacaaa
     9721 caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt cgccatattt
     9781 cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat ccaccaactg
     9841 aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga actggcggct
     9901 aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt gaattcaaaa
     9961 cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac cattcaggaa
    10021 cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa gcagttggaa
    10081 aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt tgttgattga
    10141 gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa acattgcacc
    10201 tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca gacaagaagt
    10261 cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa gttatcaatt
    10321 tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac cctgccagac
    10381 agatttttag ggagagaacc atgctgttac acattttgta tttggttggc attactgccg
    10441 aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt ggcgtaatta
    10501 ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact
    10561 atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac
    10621 tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg
    10681 cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg
    10741 gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc gttttgcgcg
    10801 atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc ggtgtctcat
    10861 ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga
    10921 ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt cttaagctgg
    10981 gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct
    11041 gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct
    11101 gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc gtgctaaata
    11161 aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt agcggtattc
    11221 ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac tgatgccata
    11281 actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata
    11341 aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc caaccggcct
    11401 caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag cgtgaaatga
    11461 actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag cggttttctg
    11521 gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac
    11581 cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc
    11641 gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga
    11701 gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt
    11761 cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc tggcccggta
    11821 gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg gtgacgaagg
    11881 gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca tttttaaccg
    11941 cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg
    12001 tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt
    12061 cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca
    12121 tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg
    12181 ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt
    12241 cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa
    12301 ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca
    12361 tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg gcaaccggat
    12421 gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc acaaagcagc
    12481 gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt
    12541 ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt
    12601 cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc
    12661 acaagatact tattaatatc gccatccata ctttcatctt cacctccgct gtagtcaggc
    12721 tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt ctgcgaggac
    12781 gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa cagctacgca
    12841 aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg
    12901 tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct
    12961 caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag cctgattcag
    13021 gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc
    13081 caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt
    13141 aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat
    13201 ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat
    13261 atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt
    13321 attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc
    13381 gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa
    13441 tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt
    13501 attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc
    13561 aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg
    13621 agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt
    13681 tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat
    13741 ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa
    13801 ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagctggaa
    13861 gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg
    13921 tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac
    13981 caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca
    14041 cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat tctggccgag
    14101 atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagatact
    14161 cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag
    14221 ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac
    14281 tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc
    14341 gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc
    14401 gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt
    14461 aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc
    14521 aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa aatcctctcg
    14581 gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg tcgcgagaag
    14641 catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg
    14701 gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg
    14761 caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca
    14821 aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt
    14881 gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt tgccgcgcac
    14941 tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg
    15001 atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc
    15061 gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc
    15121 gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt
    15181 catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc
    15241 agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg
    15301 aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag
    15361 cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt
    15421 aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca
    15481 acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg
    15541 aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg
    15601 cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct
    15661 ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc
    15721 tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat
    15781 aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc
    15841 gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat
    15901 acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc tcgtgaccgt
    15961 gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc
    16021 cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc
    16081 gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt
    16141 tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct
    16201 ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg
    16261 aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg catgcagatt
    16321 ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta cactcgcccg
    16381 acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg
    16441 gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc
    16501 tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt
    16561 gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg
    16621 gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc
    16681 gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga
    16741 aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta
    16801 gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc
    16861 tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg
    16921 ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga
    16981 cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg
    17041 caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg
    17101 gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc
    17161 cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca
    17221 cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac
    17281 tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc
    17341 accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac
    17401 gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg
    17461 gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa aataaactcc
    17521 tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc
    17581 atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta
    17641 aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcac
    17701 tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc aactggtttg
    17761 caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt
    17821 ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca cacgccatct
    17881 tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa cagcatcgtt
    17941 ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc ttccatccgc
    18001 atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg
    18061 atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg actacggtcg
    18121 ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc tgcataactg
    18181 aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc
    18241 aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc
    18301 tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag
    18361 gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg
    18421 ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc
    18481 gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac aaaacgccgc
    18541 tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga
    18601 ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg
    18661 gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc
    18721 agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc ttgatagaac
    18781 gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg agaagaccgc
    18841 cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat caccagcgca
    18901 ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc catgtgcgac
    18961 gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt tgcaccgagg
    19021 ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc catcatgcgc
    19081 caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag aatcgccagc
    19141 atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc catcgccagg
    19201 aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac gcggtaaaag
    19261 cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca gacgaaggtc
    19321 ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac cgtcaggcag
    19381 atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg tcccacatcc
    19441 ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg cgtggtggag
    19501 tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac cagaccgaac
    19561 gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc aatgaacaat
    19621 ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc ggccaacagc
    19681 cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc attttgcatc
    19741 accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc ggcacgcaaa
    19801 ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa cggatcgcgc
    19861 agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag tagcgccagc
    19921 gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa aaaggagacg
    19981 gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag cgttgctaaa
    20041 gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat actcgtcata
    20101 cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg ctttcctggg
    20161 tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa ataccaaaag
    20221 ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg gcaaacgttt
    20281 gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg ttttcgatgt
    20341 ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa aacagcgaac
    20401 tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc gcctgtcagc
    20461 atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc caggcgctgg
    20521 gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc tccggtgtgg
    20581 catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg tctattcagg
    20641 gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg aaaaccggtg
    20701 gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg gcaagttgca
    20761 ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg ccgctggttt
    20821 ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg acctccattg
    20881 gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat ctgctgctgg
    20941 caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct tacttacgcg
    21001 tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt atgggcatgt
    21061 tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg ccgctctatt
    21121 acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt atgatcactt
    21181 cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag ccagtgtccg
    21241 gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg aactcgtttg
    21301 tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac ggagtgatcc
    21361 agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg ttgatcgtgc
    21421 tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg gttctgggcg
    21481 gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc gtttctcgtg
    21541 agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt ggtctgggcg
    21601 tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac ctgctctcct
    21661 ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc ccaccagaaa
    21721 aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct ctttcaccat
    21781 tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat ttcacgggat
    21841 ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct ctgttagtgg
    21901 tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat atcagcgcat
    21961 gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac cgtttttccg
    22021 cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag cccgcgcccc
    22081 tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa ccacgccatg
    22141 tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc gcgccgctac
    22201 cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg aatagcgaat
    22261 ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca gaagccggta
    22321 aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg ctcaatgatg
    22381 ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt acgctgacta
    22441 acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt aacgccgacg
    22501 gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc gaaaaatcgc
    22561 taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt cgcttggaag
    22621 tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat ctcagcttgc
    22681 gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg tcgatgaacg
    22741 ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac gcggaatttt
    22801 ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt atggtcagaa
    22861 cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg gcaattgccg
    22921 ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg acacccggtt
    22981 ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc attgatatcg
    23041 accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac ctgacgctgg
    23101 ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc gccgccgcga
    23161 cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac agcagcacgg
    23221 tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc accgcaagtg
    23281 tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt gtgccggtga
    23341 atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc aatattcagc
    23401 ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt agcgggcaat
    23461 tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg ggcgtcgttt
    23521 ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg ccgggtacga
    23581 ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg cattcccttg
    23641 aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca gaccatttac
    23701 tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac cagtcacagt
    23761 aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt cagcagcagg
    23821 cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg ccgtgccttc
    23881 gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag tgttatcacg
    23941 cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac taccgtcgag
    24001 ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact gcacatcgcc
    24061 cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt gacggtcaag
    24121 gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca tcggcgtacc
    24181 ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc atttcagttg
    24241 cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca cacgataaga
    24301 tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc agcgtttgta
    24361 aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga agccaaaacc
    24421 tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt agttagcgta
    24481 acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg agcgggcaaa
    24541 caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca gttcgttgta
    24601 gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc actgaacatc
    24661 aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg cgaccagacc
    24721 tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat aaatcgccag
    24781 acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga gtaaatagcc
    24841 tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt taatccagac
    24901 gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt cagggaaagt
    24961 cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac agtcaaagtg
    25021 gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt ttaccgtcgc
    25081 ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc acgggggcgg
    25141 cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg gcgtcgggcc
    25201 gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt tggagacttt
    25261 ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc cataaccacg
    25321 gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc cgccgtcccg
    25381 gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct ctcccagacc
    25441 gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc gttgattatt
    25501 cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt cgccgttgcg
    25561 cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt tgccactttt
    25621 aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca aaatattgag
    25681 cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca caccgacaat
    25741 accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct gccaggtacg
    25801 ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct gttcaacctc
    25861 gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt ttccatcgct
    25921 aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt gctggcaacg
    25981 gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg tgagtgagta
    26041 gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa tcgtgaatag
    26101 cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat catatccgcc
    26161 ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt tgagcacaaa
    26221 cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact cgccgtagtc
    26281 gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca acacaccaat
    26341 cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga acatcgcgag
    26401 gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac atttccagtc
    26461 ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag tggtgagaaa
    26521 agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca tcgccccacc
    26581 gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc gccactggtc
    26641 gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg gtgcttcaac
    26701 gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca cggaaaggac
    26761 cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat taaccagtgg
    26821 catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac gccaggattg
    26881 cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc agtaagggat
    26941 attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca tagatcattt
    27001 tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc ccgaatggca
    27061 gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt cgatcggcca
    27121 acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa aacatggttc
    27181 cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata tataacatta
    27241 cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt ttctctttaa
    27301 cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc tgagaccgat
    27361 aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt tattaattta
    27421 tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca ttgtaaaata
    27481 tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt aggcggaaaa
    27541 aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc gcctccatgt
    27601 agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc aaatccagtt
    27661 ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag tttacctctt
    27721 ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt atttatttgt
    27781 atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac tttagggggt
    27841 gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata cataacaacg
    27901 gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt tagttactgg
    27961 cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca tggcattaac
    28021 cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg ctgatggcgg
    28081 cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga agtaccgctt
    28141 tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc tagccgatgc
    28201 tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc caagggaggc
    28261 aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg aggctattgc
    28321 tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg atgaaatcat
    28381 tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg ccgaaatcaa
    28441 accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc tggagaaaat
    28501 gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta ctggccgggc
    28561 agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa aagtacattt
    28621 tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg gttataccgg
    28681 aagtataatc actaagacag ctactcagat cattatgtta accggcgtac gaacacaaga
    28741 actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg agatcccggc
    28801 agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg tcattatgct
    28861 atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg gtagaaatga
    28921 tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac ttggatataa
    28981 gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc atgaacaagg
    29041 ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt ccatcagggg
    29101 tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat ggtacgctga
    29161 ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc cctgatgttt
    29221 tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg ttattatatt
    29281 atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt gacattttta
    29341 aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca aaggaatagt
    29401 tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa tgagattcac
    29461 ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat
    29521 tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc agactatatc
    29581 accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag tgcacgataa
    29641 caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga
    29701 agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg
    29761 acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt
    29821 tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac tggcggcagg
    29881 taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg
    29941 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca
    30001 ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata atcagggggc
    30061 tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt
    30121 actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt
    30181 tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac
    30241 ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga
    30301 cttctccggc tctctggata cggcagaaga ttactatgca acatactcac cattatgggg
    30361 acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca
    30421 tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat
    30481 atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc
    30541 tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt
    30601 gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga tattgcatta
    30661 gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca
    30721 ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat
    30781 tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa
    30841 cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc
    30901 tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat aaacagtaaa
    30961 aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg tgcttgtgtt
    31021 ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat tgcatccaac
    31081 ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa atcaatggat
    31141 ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc tcatactggc
    31201 gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa gacgcccaaa
    31261 atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt gtcaataaat
    31321 tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc ccaaaagttt
    31381 atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat attgttacac
    31441 cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg ccagcgacct
    31501 ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt ggtgagattg
    31561 ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg tggacacggc
    31621 cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag gcactgtgac
    31681 gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc attatttccc
    31741 ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag aagctttcca
    31801 cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg ttgtgtcgct
    31861 tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta tgaacaatga
    31921 cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag gccagttgtg
    31981 ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg tcgatgacta
    32041 ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc gcccacttct
    32101 ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac gggcaggcca
    32161 tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag cccctgacga
    32221 cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc aaggcgggga
    32281 gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc gcaggtgagc
    32341 cgaaactgtt ggcgcaggct catctggtga cgacgactga gccagacata ccagccgctg
    32401 cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg atgattttcg
    32461 ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt tggaggatgg
    32521 acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca gcggccagtt
    32581 cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag gcataaagct
    32641 gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt tcagcgagtt
    32701 tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg ctgattgata
    32761 ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct gtgtccacta
    32821 ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat atatcatctg
    32881 taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt gaatattata
    32941 actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg ttattatcac
    33001 caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc tttgtgacca
    33061 aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta ttttgctaca
    33121 tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt agttactcaa
    33181 ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca aagattgaaa
    33241 aattatccaa cctatggttt ttattaccca atagccaatt ttat
//
LOCUS       DAJTUN010000033        32122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTUN010000033 DAJTUN010000000
VERSION     DAJTUN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.033"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO470_001478"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO470_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6250772.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO470_001479"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO470_001479"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6250773.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO470_001480"
     CDS             complement(3736..4092)
                     /locus_tag="OO470_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6250774.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO470_001481"
     CDS             complement(4096..4512)
                     /locus_tag="OO470_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6250775.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO470_001482"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO470_001482"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6250776.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO470_001483"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO470_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO470_001484"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO470_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6250777.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO470_001485"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO470_001485"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6250778.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO470_001486"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO470_001486"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6250779.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO470_001487"
     CDS             10521..11504
                     /locus_tag="OO470_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6250780.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO470_001488"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO470_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6250781.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO470_001489"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO470_001489"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6250782.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO470_001490"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO470_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO470_001491"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO470_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6250783.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO470_001492"
     CDS             complement(15306..15515)
                     /locus_tag="OO470_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6250784.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO470_001493"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO470_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6250785.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO470_001494"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO470_001494"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6250786.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO470_001495"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO470_001495"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6250787.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO470_001496"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO470_001496"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6250788.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO470_001497"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO470_001497"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6250789.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO470_001498"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO470_001498"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6250790.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO470_001499"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO470_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO470_001500"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO470_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6250791.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO470_001501"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO470_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6250792.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO470_001502"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO470_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6250793.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO470_001503"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO470_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6250794.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO470_001504"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO470_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6250795.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO470_001505"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO470_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6250796.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8174 a         8244 c         7780 g         7924 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaaggtt
    32041 aaagatgttg ttctgccaat gttatgccgc tgcaccctca acttacgtta tcccaacttg
    32101 tgactattat tcggcgctcc ac
//
LOCUS       DAJTUN010000034        32008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTUN010000034 DAJTUN010000000
VERSION     DAJTUN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.034"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..330)
                     /locus_tag="OO470_001506"
     CDS             complement(<1..330)
                     /locus_tag="OO470_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6250797.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSV"
     gene            576..1202
                     /gene="gstB"
                     /locus_tag="OO470_001507"
     CDS             576..1202
                     /gene="gstB"
                     /locus_tag="OO470_001507"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6250798.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1199..2313)
                     /gene="yliI"
                     /locus_tag="OO470_001508"
                     /pseudo
     CDS             complement(1199..2313)
                     /gene="yliI"
                     /locus_tag="OO470_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2424..2807)
                     /gene="bssR"
                     /locus_tag="OO470_001509"
     CDS             complement(2424..2807)
                     /gene="bssR"
                     /locus_tag="OO470_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6250799.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3020..4345
                     /gene="rimO"
                     /locus_tag="OO470_001510"
     CDS             3020..4345
                     /gene="rimO"
                     /locus_tag="OO470_001510"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6250800.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4392..5720)
                     /locus_tag="OO470_001511"
     CDS             complement(4392..5720)
                     /locus_tag="OO470_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6250801.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(5728..8076)
                     /locus_tag="OO470_001512"
     CDS             complement(5728..8076)
                     /locus_tag="OO470_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6250802.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8254..9165)
                     /gene="gsiD"
                     /locus_tag="OO470_001513"
     CDS             complement(8254..9165)
                     /gene="gsiD"
                     /locus_tag="OO470_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6250803.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9168..10088)
                     /gene="gsiC"
                     /locus_tag="OO470_001514"
     CDS             complement(9168..10088)
                     /gene="gsiC"
                     /locus_tag="OO470_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6250804.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10106..11644)
                     /gene="gsiB"
                     /locus_tag="OO470_001515"
     CDS             complement(10106..11644)
                     /gene="gsiB"
                     /locus_tag="OO470_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6250805.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(11664..13535)
                     /gene="gsiA"
                     /locus_tag="OO470_001516"
     CDS             complement(11664..13535)
                     /gene="gsiA"
                     /locus_tag="OO470_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6250806.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13522..14487)
                     /gene="iaaA"
                     /locus_tag="OO470_001517"
     CDS             complement(13522..14487)
                     /gene="iaaA"
                     /locus_tag="OO470_001517"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6250807.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            14691..15926
                     /gene="moeA"
                     /locus_tag="OO470_001518"
     CDS             14691..15926
                     /gene="moeA"
                     /locus_tag="OO470_001518"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6250808.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            15926..16675
                     /gene="moeB"
                     /locus_tag="OO470_001519"
     CDS             15926..16675
                     /gene="moeB"
                     /locus_tag="OO470_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6250809.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(16854..17516)
                     /gene="fsa"
                     /locus_tag="OO470_001520"
     CDS             complement(16854..17516)
                     /gene="fsa"
                     /locus_tag="OO470_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6250810.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            17647..18546
                     /locus_tag="OO470_001521"
     CDS             17647..18546
                     /locus_tag="OO470_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6250811.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18552..20984
                     /locus_tag="OO470_001522"
     CDS             18552..20984
                     /locus_tag="OO470_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6250812.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21130..21945
                     /gene="ybiV"
                     /locus_tag="OO470_001523"
     CDS             21130..21945
                     /gene="ybiV"
                     /locus_tag="OO470_001523"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6250813.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQGIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22097..23362
                     /locus_tag="OO470_001524"
     CDS             22097..23362
                     /locus_tag="OO470_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6250814.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23603..25195)
                     /locus_tag="OO470_001525"
     CDS             complement(23603..25195)
                     /locus_tag="OO470_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6250815.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25414..26334
                     /gene="ldtB"
                     /locus_tag="OO470_001526"
     CDS             25414..26334
                     /gene="ldtB"
                     /locus_tag="OO470_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6250816.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26392..27510)
                     /locus_tag="OO470_001527"
     CDS             complement(26392..27510)
                     /locus_tag="OO470_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6250817.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27507..27974)
                     /gene="mntR"
                     /locus_tag="OO470_001528"
     CDS             complement(27507..27974)
                     /gene="mntR"
                     /locus_tag="OO470_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6250818.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28160..28288
                     /gene="mntS"
                     /locus_tag="OO470_001529"
     CDS             28160..28288
                     /gene="mntS"
                     /locus_tag="OO470_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6250819.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28560..30143
                     /gene="opgE"
                     /locus_tag="OO470_001530"
     CDS             28560..30143
                     /gene="opgE"
                     /locus_tag="OO470_001530"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6250820.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30191..30706)
                     /gene="ompX"
                     /locus_tag="OO470_001531"
     CDS             complement(30191..30706)
                     /gene="ompX"
                     /locus_tag="OO470_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6250821.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(30759..30824)
                     /locus_tag="OO470_001532"
     CDS             complement(30759..30824)
                     /locus_tag="OO470_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250822.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31059..31946
                     /gene="rhtA"
                     /locus_tag="OO470_001533"
     CDS             31059..31946
                     /gene="rhtA"
                     /locus_tag="OO470_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6250823.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8011 a         8520 c         7745 g         7732 t
ORIGIN      
        1 taccgatgaa ccacgcagtg ccggatttcc cgtcgcccag gcatctttac cgaccgtcac
       61 catatcggtg agtttaatct tatcggcctt aagcgcctgc ccaaccacat agctggtcat
      121 gattttagtc aggctcgcag gatccagttt ctcatccgca ttgccttctg ccagcacttt
      181 accgctggcg taatccatta aaatccatgc acgcgcatcc acgctcggcg cttcaacggt
      241 ttgttcggcc gcgaatgccg ttggggcaaa aaggaataaa aatgcagaac ccgctgcaag
      301 accacgaagg agagaggagt attgcgtcat aaatgccacc cgagtatcca ttcaaaaaaa
      361 tacgttatat cgccgtatga taagttcggt gattaataac gcacacacaa acttaaagaa
      421 accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg
      481 tgtcattttt ttgccagctg ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt
      541 tcagtctgtg gctgcctgtc ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata
      601 attcaactaa cgtaaaaaaa gtattgctga cgctcgaaga actggaacta ccttatgagc
      661 aaattctcgc gggccgtgag tttgggataa atcacgatgc tgattttctg gcgatgaacc
      721 ctaacgggct ggtgccgttg ttgcgtgacg acgaaagtga tctcattctt tgggaatcaa
      781 acgccattgt ccgctatctg gcggcacagt acgggcaaaa acgcctgtgg atcgactcac
      841 cggcacgtcg tgcggaagcg gaaaaatgga tggactgggc aaaccagacg ctcagtaatg
      901 ctcatcgcgg gatcctgatg ggattagtca gaacaccacc ggaagagcgc gatcaggccg
      961 ccattgatgc cagttgcaaa gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg
     1021 caaaagtaaa atggttctct ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt
     1081 tcatctacaa tttgtttaac gttggcctga cctggacacc gcgtccaaat ctgcaacgct
     1141 ggtatcagca actcactgaa cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct
     1201 aattgcgtgg gctaacttta agtaattccc cactggactc gtcggtgaga acgtataaat
     1261 aaccgtcggg tccagtgcga acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc
     1321 catcttctgt cactttgtcg ccgttgacgc tcatcacaat gacatcttta tctttcagct
     1381 cccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga aggccatgcc
     1441 gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc
     1501 ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg ccagcggcca
     1561 gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg ggccatgttc
     1621 attcagccac agtgcattac tccacggatt catcgccatt ccttgcggat tacgaatgcc
     1681 ataagaccag atctcggcgc gcgcaccgga ttcctttata aaaggattat catccgggat
     1741 ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat cctgcgccgt
     1801 cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt cgaataccag
     1861 ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcggaaga cggtgcggaa
     1921 gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag ttccggcttt
     1981 gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag caaaatcagg
     2041 cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg gaactccgga
     2101 aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt
     2161 gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc
     2221 ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag aaagacaaat
     2281 aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac tctggcttaa
     2341 agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac atgaaggggg
     2401 agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc
     2461 tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt gacggtaata
     2521 acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc taatgcttct
     2581 tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa ccgcagcgat
     2641 ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt gctttcgctg
     2701 acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg
     2761 tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc
     2821 tctttccagc cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa tgctgtccag
     2881 gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg
     2941 agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac ctttcctgta
     3001 actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg gctttgtttc
     3061 ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac tccgcactga
     3121 aggttatgac gtggtaccga gctatgacga tgcgaacatg gtgatcgtca acacctgcgg
     3181 ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa
     3241 cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca
     3301 cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg agcacgttca
     3361 tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag aacaaggtgt
     3421 gaaactgacg ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta atcaccgctg
     3481 caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt
     3541 gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg tgatctcgca
     3601 ggatacttcc gcctatggcg ttgatgttaa acatcgtact ggcttccaca acggcgagcc
     3661 ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga tctggacacg
     3721 tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga tggcagaagg
     3781 caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca ttctcaaact
     3841 gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat
     3901 ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg agacggaaga
     3961 agatttccag atgctactcg acttcctgaa agaagcacgt ctggatcgcg ttggctgctt
     4021 taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg ttccggaaga
     4081 agtgaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt ccgccgagcg
     4141 cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg acgaagaagg
     4201 cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg
     4261 tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta
     4321 cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt tgtccggcct
     4381 gcgaataatg attactgcca gctaatctgt gccctgccgt ttttcttact cttatacagc
     4441 cgctcatccg ccagcatttt caagcgttcg acggtagtac attcatttga atgcgctaca
     4501 ccgatactgg tgcttaaatt aatgcttccc acctggaaat gaacgatttc ttttagtatc
     4561 gatagcaact cttccagcgt gccgccagta taaatcaccg caaattcgtc acctccgaag
     4621 cggtagactt tacctttatc taccgggatc tgtgatttaa ggattttcac cactttcatc
     4681 aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg
     4741 tcaaacagac acaaataacc gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat
     4801 tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat actgtagagt
     4861 tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta
     4921 ttaatgatgg cttctttctc cacaatacca cgattaatca ctctgcgtac cagatagagg
     4981 caactggcac ccaatatcac tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc
     5041 cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa ataggcgaag
     5101 tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cattatattc atcaccggtc
     5161 accggtttac cgttcttaaa tagagagaac atcaggttct cgctgtccat caccagcgat
     5221 tttgacagta aagaaaaacg ctccagacca aacaaaaata tcagactacg attgtgagtt
     5281 accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc tgtatttttg
     5341 acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc gaactccttt
     5401 acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa ataagagggg
     5461 gaaaccgagt cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt
     5521 agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt tgccaccgca
     5581 aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac catttgagtg
     5641 tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac tgtaagtacg
     5701 aacttattga ttctggacat acgtaaacta ctcttttact aattttccac ttttatccca
     5761 agcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac atcctaattt
     5821 aattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg ggatccggtt
     5881 atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga aaatatcgcc
     5941 ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac gtttcaggtt
     6001 tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac gcagttgttc
     6061 aatattgaga taaaaatgtt cattaattcg ctcgatcacg gtttcagtga tctcaacaca
     6121 gaggctgttc gccttcaggc cattttcttt catgatggtg gtaaaacgct catgaaagtg
     6181 cggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat ccttcagcga
     6241 cgcacgttga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct gataacccag
     6301 ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc
     6361 ccgctccgca gaaacccaac ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat
     6421 atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat ggcgaatatt
     6481 acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac agaaaataaa
     6541 ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt ctgcgtaact
     6601 ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc gcagattgcg
     6661 gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa atgtctgtac
     6721 attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat cgcagcattg
     6781 cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc gttcatcaac
     6841 accaaacacc gcctccacgg cttccagacc acacagtttg atgatcccca cagccagata
     6901 ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt cctgatcgta
     6961 agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca tttccgcgat
     7021 cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca gagcctcgat
     7081 ctgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca tttcagaaaa
     7141 ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga taataatgaa
     7201 cgcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc caggataata
     7261 aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa tatactccgt
     7321 atcgacagaa tcaataatat tataattacc ttcgctatta taaataatca gtttttcacg
     7381 agcgagttga ttcttggcaa tgatctcatg accttgtttc aaaaatagaa cttcatcatt
     7441 agcgctgatt tgatcaagat actcgttcag ataccgccag tttaaatcga catgaatttt
     7501 atctaccgct aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag agatacattt
     7561 tttacccgga cgatcaaagc aattgtatgc cggattccag ctgttgttag agtcatctga
     7621 agccaccgac cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc gccctgactc
     7681 gtcgacaagc ggtccattta cttgttcctg atgataaaca acgattttat cattcgccct
     7741 ccgcgaaaag agataatatt tattatcatc aagtaccagt cccaccgagt ctaaaaatgg
     7801 catcatttcc attttcagaa tcacggcgcg ttgaatctcc tctgtatttt ttaataaata
     7861 cagtgagtcc tttaatgtca gggccgaacg ttcaatatct ttaaagaaat agccaacgtt
     7921 tttctgaata agttcaaagc ggctatcggc acgttttacc gcgcgttccg ataccagttg
     7981 atagttaatc atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa ataaaattat
     8041 cagccttatc tttatctttt cgtataaact cagcatgtta atacatttaa caaaataact
     8101 atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa ccaaattacc
     8161 cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat ttcctgtgag
     8221 gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg gatccagcgc
     8281 atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa tagccagggc
     8341 cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat tgagcattgc
     8401 tccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc tggcggcaga
     8461 gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagaagaga cggtcccagg
     8521 caggatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg ctgactcaat
     8581 aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa acgcggggat
     8641 ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca ccgcaacaac
     8701 agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag
     8761 ccgatcccac cagccttcat aatatccagc caacaagccc agcaacgtcc ctatcgccac
     8821 accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg
     8881 gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt
     8941 attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa
     9001 aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca tatgctgacg
     9061 gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc tggtcaggtt tgaccagtgg
     9121 catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta cttgtacctg
     9181 atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag aataaattcc
     9241 agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc tacagagtca
     9301 acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat agaaccaccg
     9361 agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc gttgcgtagc
     9421 ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt acgcatataa
     9481 tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc gcgccatcac ggcggccact
     9541 gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc tcccacagta
     9601 ggcagccagc ccagttcaac ggagaatacc tgaattaaaa acatccccag cgcaaatgcc
     9661 ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca acgattacgc
     9721 cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac catactggtt
     9781 atggtcagcc acagcgttgg cataaagcgg ctggcaatct catcggcaac cggacgacgc
     9841 gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg ccagaactgg
     9901 tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac ctgcgcatca
     9961 gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatgtggac aaataaaaac
    10021 accagcaccg agacgataaa cagcgtcgga atcaacccca gtaatcgttt gataacgtaa
    10081 ttaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgg
    10141 tatctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc agtttttcta
    10201 ccaccagcgg gatccacggc gattcttgcc agatgatatc ctgcgccgcc ttatataagc
    10261 gggtcttttc cgccggatca ttcgttttca gtgcctgagc caggaagtca tccacctgtt
    10321 tattgctgta aaacgcggta ttaaacagcg tcggtggcca gttttgcgag gcaaacagcg
    10381 gcgatagtgc ccagtcggct tcgccggttg aagccgacca gccagtgtag aacatccgca
    10441 cgccactctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg
    10501 cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt
    10561 tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg ttgggatatc
    10621 ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt ttataacttt
    10681 gcgcgtaggc gatacttggc ggtaccacac cagtagctgg cgttgcatag cccgcaaagg
    10741 caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg accttcgggt
    10801 tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg
    10861 ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag ggaatgggga
    10921 aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg
    10981 gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt
    11041 tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg gttcccaccg
    11101 gatgaaaacc aatctccttg ccatattttt ccagcgctgt cggtgaaatc atcgcggtcg
    11161 ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagggta atctttaccg
    11221 ttgtcggatc gatcgcctcc gttttggcaa tattcttata caggttatag cgtttaagat
    11281 gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccactgcg ttgaaatcgg
    11341 tgccatcctg gaatttaatt ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg
    11401 aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc agaccgaaca
    11461 gcccctggta aaacgatttc gctacggcct gagataacgt gtcattcgcg tcatacggat
    11521 cgagcgtggt gaaattcgat cctaccgcca ccaccacatc tttggcagcg aatgcacaag
    11581 atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt acagctcttg
    11641 ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga ttgtggttgt
    11701 gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc ttcgccacgc
    11761 agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg ttgtcgggac
    11821 ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg cgggttttcg
    11881 aagaccgcgc accgtgaacc aatttcaaca atttgcccga gatacatcac cgccacacga
    11941 tgactaatcc gctctaccac agccatatcg tgggagataa acagatacgc aatgccgaaa
    12001 tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac atccagcgcc
    12061 gaaacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg agcaatgcag
    12121 atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac
    12181 aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa
    12241 ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa
    12301 gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag tttgccaggt
    12361 gacaaggtat cgattcgctg accgttaaag ataatttcgc cgccctgcga ttcgaccagg
    12421 cgcagcaacg cccgcccggt agtggattta ccgctgccag actcacccac cagcgatagc
    12481 gtttcgccag gccagagatc aaaactgact ttctcaacgg catgcacttc ccgcgttacg
    12541 cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac ccgtaaaaca
    12601 ggttcgccat ccaccaccgt tttctgctcg atggggggtt cctgtttcgc tggatgttca
    12661 agcgatatca atgggaaacg tcggggataa tctaaccctt tcatcgcacc aagttgcgga
    12721 acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg
    12781 gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc aatctctgcc
    12841 accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt ttgcaatact
    12901 ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt tggctcatcg
    12961 gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc
    13021 ccgccagaga gttgatgcga ataacgtgaa agaatggttt gtgcctccgg aatgcgtacc
    13081 tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga
    13141 cgaattgatt cggcaatctg ttcacccaca gtaaatactg ggttcagcga tgtcatcggc
    13201 tcctgaaata tcatcgccat atccgcaccg cgcacatggc gcatttgtgc agcgctctgc
    13261 tcgctaagtt caatcacatc acgactgcgc cgccgcaaca gcattttatc gcactgtact
    13321 aaaccgcccg cctgttccaa cagacgcatc aatgccagcg cagtcactga cttaccggag
    13381 ccggattcgc ctacaattgc cagcgtctca ccgcgttgca gactaaaaga gagattgcgg
    13441 accgcagcta ttttctgctg gtcctgcata aaggcaatat taagattttc aaccgccagc
    13501 acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt cacggtagat
    13561 accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa
    13621 cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac cgccaagcgc
    13681 agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc cgccgtaatc
    13741 cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc ctgtgccggt
    13801 acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta agggactatc
    13861 gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg ccgccagatt
    13921 gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt catccagtgg
    13981 cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc gctgccagta gttgttcata
    14041 acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc atgccatgag caaacgcaaa
    14101 attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca ccagccgggc
    14161 ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt tcagggtgtt
    14221 accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat
    14281 tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca ccacatccag
    14341 cgcactttcg ccggcttcca gcattttctg cccggtttca acaatggcag acaacgcctc
    14401 gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc
    14461 atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc tcgccacttt
    14521 tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgaatat agaaagatgg
    14581 atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag tctatggcgt
    14641 ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc atggaattta
    14701 ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc gttaccccac
    14761 tgaccaccca ggaaacgctg ccactggttc agtgttttgg tcgtattctg gcgagcgatg
    14821 tcgtttcgcc actggatgtc ccggggtttg ataactccgc tatggacggc tacgcggtgc
    14881 gtttagccga tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc
    14941 agccatacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc ggtgcgccgg
    15001 tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg gacaatggcg
    15061 tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct
    15121 ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa
    15181 ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt
    15241 ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa atctacgaca
    15301 ccaaccgtct cgccgtacac ctgatgttag aacagctggg ttgcgaggta atcaacttag
    15361 ggattatccg cgacgatcca catgccctgc gcgccgcatt tattgaagcc gacagccagg
    15421 cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac accaaaatga
    15481 ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca ggtaaaccgt
    15541 tcgcgttcgg taaactcagc aatagctggt tctgcggcct gccgggcaac ccggtttcag
    15601 cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc ggcaacaccg
    15661 ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag
    15721 gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga
    15781 cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc aactgcttta
    15841 tcgtgctgga acgcgatcgc ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt
    15901 ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta
    15961 caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc taaaagattc
    16021 tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt atctggcaag
    16081 cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct cgaatctgca
    16141 acgccagaca ctgcacagtg atgccacggt cgggcaaccg aaggtggaat ccgcccgtga
    16201 cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac tgctagatga
    16261 cgcagaactt gcagcattga ttgctgaaca cgatctggtg ctcgactgta cggataacgt
    16321 tgcggtacgt aatcaactga acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg
    16381 cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg
    16441 ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta acctgcgtag aagcaggcgt
    16501 gatggcaccg ttgataggcg taattggttc gttgcaggca atggaagcga tcaaactgct
    16561 ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca
    16621 gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc
    16681 acctccaagc ctgataagac gcgacaagcg tcgcatcagg catttgctcc agacgccaga
    16741 tgcggcgtga acgccttatc tggcctacgg ttcgggcaca gatttccaag cgcggcaggc
    16801 atcggtgcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc
    16861 gacgttctgc caaacgctcc ctgccagtcc tgctcaaact tcgtcacagc ggcttccacc
    16921 gccggatagc taatcatctg ttgtgccaca tccagtggca gagtaattga ttcacatccg
    16981 gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc
    17041 tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat gccgctaccg
    17101 ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac
    17161 agcagccctt gtgccgcgcc atataccgcg gttcccagcg tcggaatccc ttccgctttt
    17221 aacatcttaa tcgctgccag cccctcggct gtcaccggaa ctttcaccac gatatccgca
    17281 ataatagaac gcagcttaag cgcgtcattc accatccctt cagcagtggt agccattacc
    17341 tgggcaaaca gacgcccctg accgcccatc gcttcatgaa gttgcggaag cacaacatcc
    17401 agcggttttt tacccgcggc gataatgctt gggttagtgg tcacacccgc cagcggaaaa
    17461 atacgtgaca gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc
    17521 atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt
    17581 tgacgcacat caatctgact ttcattcgaa agtattttaa tgtttgaacg aaacagagag
    17641 gcaactatga ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg
    17701 gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc
    17761 gcccgtacgc aggatctgct gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc
    17821 gctaaggccg cgccggaagt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa
    17881 aagttaaccc cggagcatct gacggcgtta accgactgct gtccgacaca ggcgttaacc
    17941 gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga ccgttctgcg cgataaaccg
    18001 ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg gtgaaccctt catgcagccg
    18061 gaaatggcga cggcgctgct gcaagcctgc cacgaggcag gcattcatac tgcggtagaa
    18121 acctgtctgc atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt
    18181 cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc
    18241 gccagggtgc tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc
    18301 gtgccgctga ttcagggctt taatgccgac gaaacctctg taaaagccat taccgatttt
    18361 gccgccgacg agctgcacgt tggcgaaatt cattttctgc cctaccacac gctgggcatc
    18421 aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca
    18481 gagctgctcg attttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga
    18541 ggataacaac catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca
    18601 aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac cgagcgcgcg cagcactata
    18661 ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg
    18721 cgcatcacct ggcgaatcgc accatctgga tcaaacacga tgagttgatc attggcaacc
    18781 aggcaagcga agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa
    18841 aagagattga tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca
    18901 aacgtgttct gcatgaagtg tgcccatggt ggcgcggtca gacggtacag gatcgctgct
    18961 atggcatgtt taccgatgag caaaaaggtc tgctggcgac cggcatcatt aaagcggaag
    19021 gcaatatgac ctccggcgat gcgcacctgg cggtcaattt cccgctgctg ctggaaaaag
    19081 ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc
    19141 tggaagattt gcacggcgaa cagttcctga aagcgattga tatcgtgctg gtggcagtca
    19201 gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat ggccgcgacc gaaacccgcg
    19261 aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg cgatcttatc gcccaccagc
    19321 cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccagttg attttgcaga
    19381 ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga tcagtatctc tacccgtact
    19441 atcgccgcga cgttgaactg aaccagacgc tggatcgcga acacgccatc gaattgctgc
    19501 atagctgctg gttgaaattg ctggaagtga acaaaatccg ctccggctcc cactcaaaag
    19561 cctctgcggg aagtccgctg tatcagaacg tcaccattgg cggacaaaat ctggttgatg
    19621 gtcaaccaat ggacgcggtg aatccactct cttacgcgat cctcgaatcc tgcggtcgcc
    19681 tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt aacgatttcc
    19741 tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat gccggcattc aacaacgacg
    19801 aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct tatgactacg
    19861 cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca
    19921 tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca
    19981 ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc ggcaggtaac ttcaacaact
    20041 tcgatgaagt gatggacgcg tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg
    20101 aaatcgaata tgtcgtcgac accatgctgg aagagaacgt gcacgatatt ctctgctcgg
    20161 cgctggtgga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg
    20221 actgggtttc tggcctgcag gtcggtattg ccaacctcgg caacagcctg gcggcagtga
    20281 agaaactggt gtttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg
    20341 atgacttcga tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga
    20401 agtacggcaa cgacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata
    20461 tcgacgaact gaaacagtac cataacccac gctacggtcg tggtccggtt ggcggcaact
    20521 attacgcggg tacgtcgtct atctccgcta acgtaccgtt tggcgcgcaa actatggcaa
    20581 cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg cgctagtccg gcctctggta
    20641 ctgaccatct cggccctact gcggtcattg gctcagtggg taaactgcct acggcagcga
    20701 ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc aacgctggag aacgaatctg
    20761 acaagcagaa actgatgatc ctgctgcgta ccttctttga agtgcataaa ggctggcata
    20821 ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt
    20881 accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt cttcaccgcg ctctctccag
    20941 acgctcagga cgatatcatc gcccgtactg aacatatgct gtaatctctt cggcccggct
    21001 gcggcgtcgc cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat
    21061 ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg
    21121 agcacaccta tgagcgtaaa agttatcgtc acagatatgg acggtacttt tcttaacgac
    21181 gccaaaacgt acaaccaacc acgttttatg gcgcaatatc aggaactgaa aaagcgcggc
    21241 attaagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag
    21301 ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag
    21361 caactgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta
    21421 aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat
    21481 gcccccgaag catttgtcgc actgatggca aaacactacc atcgcctgaa acctgtaaaa
    21541 gattatcagg ggattgacga cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa
    21601 atcccgttag tgatcgacaa actacacata gcgctggatg gcattatgaa acccgtcacc
    21661 agtggttttg gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg
    21721 cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt
    21781 aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa
    21841 aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat
    21901 gtgattcagg ctgtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc
    21961 catgcgctcc ggttttccct tctttttaca tcctgtacat cacatcaaat cattaaactt
    22021 gcattaactt atttataact tgtattgtca catgcaaatt agcatagttt cgaatgaaag
    22081 ttaatgcgag gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa
    22141 agcagctatc cgtcagatga agcacgcgct gcgggcgcag cttggcgatg tccagcagat
    22201 ctttaatcag ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc
    22261 acagggcgat gccgtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac
    22321 tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc
    22381 ccgcgaacaa gcgctaggct gggatcagtc gatgctggac tatctgaacc gcaaccgctt
    22441 tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc
    22501 cgagatttac cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat
    22561 ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg
    22621 gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc cgccgccttc cgcccggaac
    22681 gacctccaaa ggtcttggag cgcataccga ctccggggcg ctggaacgct ggctgcttcc
    22741 agcgtatcag cacgttttcg ccaacgtctt taatggcaat ctggcgaaat acgatccctg
    22801 gcatgcggca catcgtacgg aagttgaaga gtacacggtg gacaacacca ccaaatgttc
    22861 cgtgtttcgg acattccagg gctggactgc gctctcggat atgctgcctg gtcaggggtt
    22921 gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga
    22981 tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga
    23041 gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga
    23101 ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg
    23161 ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc
    23221 gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga
    23281 ctatgaaaca aactgggaag gacgctttac gcttgccgac ctcaacattc acggtaagcg
    23341 agcgttgggc atggatgttt gagagttaat ggaagtgaat tgatgtggga ttaagaaaat
    23401 cgattcgacg ttactcagat tgctgacaag tgcgctttct taatgcctga tgcggcgtta
    23461 acgccttatc cggcctacaa agtcctgcag attcaatata ttacaggtac tgcgtaggcc
    23521 tgataaacgt agcgcatcag gctattttgc atttgtcatc agccgtcgtc accccgacag
    23581 tatcggggtg acggataatc acttactcga tccctttact acgcaggtaa tcttcgtaat
    23641 taccgctaaa gtcgatcacg cgttccgggg tgatttccag aatgcgggtc gccagggagc
    23701 ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca
    23761 tgttcagtga ctcgatggat tccatatcca ggtggttggt cggttcgtcc atgatcagaa
    23821 tgttcggctt ctgcatcatt aacttaccaa acagcatccg ccctttttcc ccaccggaaa
    23881 gcactttagc tggctttttg atgtcgtcct ggctgaacag caaacgaccg agaatgctgc
    23941 gtaccgcctg ctcgtcatcg ccttcctgct tccactgact catccattcg aacacggtca
    24001 gatcattttc aaactcatat tcgtggtcct gggcgtagta accaatgcgc gcgttttcag
    24061 accattttac ggtgccgctg tccggttgca gatcgcccac cagcgttttc agcagcgttg
    24121 atttaccgac gccgttggta cccagtaccg caagtttttc accgacctcc agcagcaggt
    24181 tgagattttt aaacagtgga ccgttatcaa accctttggt cagaccttcc acttccagcg
    24241 cgttacggaa cagtttctta tcctgttcaa aacggataaa cgggttctga cggctggagg
    24301 ctttcacctc ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag
    24361 atttcgaggc gttggcgcta aagcggctaa cgaaagattg caactcagca atctgcgctt
    24421 tcttcttggc gttatcggcc agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact
    24481 catcgtagtt acccggataa acgcgcagct cgccgtaatc cagatccgcc atgtgggtac
    24541 agaccatgtt aaggaagtga cggtcgtgcg agatgatgat catggtgctg tcacgctcgt
    24601 tcagcacctg ttccagccag cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga
    24661 gcaggagaat atccggctca gcaaacagcg cctgcgccag aagcacacgc agcttccagc
    24721 caggagcaac ttcactcatc gggccgtagt gctgttccac tggaattccc acgccaagca
    24781 acagttcacc ggcgcgagct tccgcagagt aaccgtccat ttcgccgtat ttcacttcca
    24841 gatcggccac tttatagccg tcttcttcac tcatttccgg caaagcatag atgcggtcgc
    24901 gctcctgctt cacttcccac aactctttat gccccatgat caccgtatcc agcacagtga
    24961 actcttcaaa ggcaaactga tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg
    25021 aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat cttcataaag gtggatttac
    25081 cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc gccgaatttg acggaaatgt
    25141 tttcaaacaa cggcttactg ccgaactgca tggtgacgtt actggaaact aacacaggcg
    25201 tatcctgaaa agagatatga caaaccgcgc attatgccat aagtctgaaa gaagatcctg
    25261 ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg
    25321 atttcactgt gagctggaat gaacttataa tgcgcttcca atactctaat attctcaacc
    25381 caatggcctg ccaggcacaa aatctcgctt aacatgaata tgaaattgaa aacattattc
    25441 gcagcggcct tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac ttatcctctg
    25501 ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga tcaccattcc tgaaggtaac
    25561 actcagccgc tggagtattt tgccgcggag taccagatgg ggctttccaa tatgatggaa
    25621 gcgaacccgg gtgtggatac cttcctgccg aaaggcggta ctgtactgaa cattccgcag
    25681 cagctgatcc tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt
    25741 ctgtattact atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg cattggtcag
    25801 ttaggcaaag atacgcctat caactggacc accaaagttg agcataagaa agcaggcccg
    25861 acctggacgc cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga accgctaccg
    25921 gctgtcgttc cggcaggtcc ggataacccg atgggtctgt atgcactcta catcggtcgc
    25981 ctgtatgcta tccatggcac caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt
    26041 tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc
    26101 cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg agccagacgg cagccgttat
    26161 attgaagtcc ataacccgct gtctaccacc gaagcccagt ttgaaggtca ggaaatcgtg
    26221 ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc agccagatgt tgaccaggtt
    26281 gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg ttcgcctgaa ttaatcgtca
    26341 aaacgtaaaa caaaggcgga ctcataatcc gcctttttta tttgccagac cttaccaggc
    26401 cgggatcata actaacaaaa cgtatcccac cagcgccgcc cacaacagca tcggtattga
    26461 atagagatgg aaacgccacc agatgcggcg atcgttcgcc atacgtagcg caatcaaatt
    26521 tgccagcgat cctggtaata aaccaaagcc ccccacgttt accgcccatg ccagtaataa
    26581 agacggaggt acataattga gcagcaaaat ggtactcggc acgttactga tcacctgcga
    26641 taaaccgact gccgttaacc ataacccggg ttcagataaa tgactcacgt tacccaacac
    26701 gccttgcaac gctggaagct gggtcagtaa atggacgtcg ataaacatcg ccataaacac
    26761 cagcagcaac gtccagtcca cactcaacac cacgcgacgc gccagcagca caaagcccgc
    26821 cgccacaatc accagtcccc acagctcttg tttgaactcc agcgccgtca gaaagacgat
    26881 atacagcccc agacaattcc acaccagccg cggtttccac tccggtgttt gcacccccgt
    26941 atggtattgc agcgcctttc cagggaaaca acaccagcac aacagcagga gtgtcagcat
    27001 cattgcgcca gccatcggtg ccatttgggc aataaatccg gcaaacgaaa gaccagaacg
    27061 tccccagata agaatatttt gcgggttgcc aattggcgtc agtagcgaac cagcgttgac
    27121 tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc tcacacagtc tttttagcgt
    27181 gatagtcagc ggaacaacaa taaacagcgc gacatcgttg gtcagaaagg tagaaagcag
    27241 cgccgccgcc agcaccataa acatcgccag ccgacgctcc gtagcaaagc ggcgcaccat
    27301 tttgcgcccc agcacatcaa aataaccgct taactccaca cctttggtca gcagcatcag
    27361 gccgcttaag gtgatgatgg tgtgccagtc gatagcagca ggccaggatt tcggtgcaaa
    27421 gggcacgaaa aagcttaatc cgataccaac aagaattaat aactgaaaaa aacgatcgcc
    27481 ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt gtttctgggt aaacaaacga
    27541 aaagcgtcga gcgtctcttc actaacatgg tgctccatgc cttccgcgtc gcgacgggcg
    27601 atttccggac tgacgcccaa caccaacaag aaattttcga ctatctgatg tcgctcgcgg
    27661 ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca cgccacgcca ggggatcatt
    27721 tcaatcagcc ccatggttgc cagccgctta agcattttag ccaccgtcgg ttgcgaaact
    27781 cccagacgag cagccatgtc cacctgacga gcttccccca cttccctgat caagtcagaa
    27841 atcagctcaa cgtaatcatc aataagctcg cgccgatgcg cctctctgac ctggcggaac
    27901 ccttcaacgt gctcttccac gttcactaac tgcgtcactt tttttgctat tggcgtacct
    27961 gcgcgacgac tcattgtgct tcctcattat ggtgacgcat acatagcgtt ctcatttcgt
    28021 gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt aacgttttag caatagctat
    28081 ataatatagc ctgtgctata tctgtatgta atgcaatcat ccctcaagga tcgacgggat
    28141 tagcaagtca ggaggtctta tgaatgagtt caagaggtgt atgcgcgtgt ttagtcattc
    28201 tccctttaaa gtacggttaa tgctgctctc tatgttgtgc gatatggtca acaacaaacc
    28261 gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt acgccgcttc actcctgctt
    28321 ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact ttttgtcgga tttgcaatcc
    28381 ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg gcggctcgct ctatcttctt
    28441 gcagccctgc gtatattgcg gctcgcggat gcggacccct ttcccctctt cacgcactct
    28501 tgcaggtatt gacccttgac gccagggtaa gcacatggcg tttgttacga tagtggcata
    28561 tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg ggtatttagc ccgtggactg
    28621 cgttctactt tttacagtcg ctattaatta acctcggctt aggttacccc ttcagtttgc
    28681 tctacaccgc tgcgtttacg gctattttgc ttttgctatg gcgaacattg cctcgcgtac
    28741 aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg ttatttccct tttgctcagg
    28801 cctacggcgc gcctaatttc aatacattgc tggcattgca ctccaccaat atggaagagt
    28861 cgaccgaaat cctgacgatt tttccgtggt acagctacct ggtcggctta tttatttttg
    28921 cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga aaaagcgcgc tggaatacct
    28981 tcgacagcct gtgtctggta ttcagtgtgg cgacattttt tgttgctccc gtgcaaaacc
    29041 tggcctgggg tggcgtattt aaactgaaag atactggcta tccggtattt cgttttgcta
    29101 aggatgtcat cgtcaataat aacgaggtga ttgaagagca agaacggatg gcaaaacttt
    29161 ccggaatgaa agatacctgg acggtcactg ccgttaagcc gaagtatcag acctatgtgg
    29221 tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc ctttggcggt cactgggaca
    29281 ataccccgtt tgccagcagc gttaacggtt tgatatttgc tgactacatt gctgccagtg
    29341 gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt agtcgatggc aaaccacagt
    29401 ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt ccagacctgg tggttttcca
    29461 accagggcca aatcggcgaa tacgatactg ctatcgccag catcgccaaa cgtgcagatg
    29521 aagtgtactt cctgaaagaa ggtaattttg aagcagataa aaacacgaaa gacgaagcgt
    29581 tactggatat gaccgctcaa gtgctggcgc aagagcactc gcaaccgcag ctgattgttc
    29641 tgcatctgat gggctcgcat ccgcaggcct gcgacaggac acaaggcaaa tacgaaacct
    29701 ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat gacgcaaacg gacgatttac
    29761 tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag cttctctctg gtttactttt
    29821 ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt gcaatacctt gcccatgatg
    29881 ataaatatca gcaaaatttc caggtgcctt ttatggtcat ttccagcggc gataaagcgc
    29941 atcgggtgat taaagcccgc cgctcagcca atgacttctt aggctttttc tcgcagtgga
    30001 cgggaattaa agcgaaggaa ataaatatca aatacccgtt tatatctgag aagaaagccg
    30061 ggccgatata catcaccaac ttccagttac agaaggtgga ttacaaccat ctcggaaccg
    30121 atattttcga cccgaaacct taaaaacaaa aatccgcccc gagaggcgga tttttatatc
    30181 accaaagtga ttagaagcgg taaccaacac cggcaatcca agtgcctacg tcaacgctac
    30241 gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg ttgaactgca
    30301 gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag
    30361 tggtctggaa tttaccataa cccacaccca ctacaccgta gatgcttgcc cagtcgttaa
    30421 tgcggtaagc cggaccagca gtgatgccgt agtactggtt tttgttgtag tcaccagagc
    30481 ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat cacacccagc gggctgttgt
    30541 cttcttcata gcggtatttc aggttgaaac cgcccatttt gttcatttgg ccctgagcgt
    30601 cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc tacggaagta cctgcggtga
    30661 aagccagaac tgcggccagt gctgaaagac atgcaatttt tttcataacc acctcaaatg
    30721 tgattcaaat aagtcctaag ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat
    30781 ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt ccatacaccg ctatccatct
    30841 aaatttaaat cactttttca gagaactgcg taagtattac gcatgttttc cctgtcattc
    30901 atccagatta ttcctaatca ccagactaat gattccatca atcctggcgc attttagtca
    30961 aaacggggga aaattttttc aacaaatgct caaccagcat tgggtatatc cagtacactc
    31021 cacgctttac ttaagtctag atatttgtgg gagaaaggat gcctggttca ttacgtaaaa
    31081 tgccggtctg gttaccaata gtcatattgc tcgttgccat ggcgtctatt cagggtggag
    31141 cctcgttagc taagtcactt tttcctctgg tgagcgcacc gggtgtcact gcgctgcgtc
    31201 tggcattagg aacgctgatc ctcatcgcgt tctttaagcc atggcgactg cgctttgcca
    31261 aagagcaacg gttaccgctg ttgttttacg gcgtttcgct gggtgggatg aattatcttt
    31321 tttatctttc tattcagaca gtaccgctgg gtattgcggt ggcgctggag ttcaccggac
    31381 cactggcggt ggcactgttc tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg
    31441 cggttcttgg tctgtggttc ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa
    31501 ccggctgtgc gctggcactg ggggccgggg cttgttgggc tatttacatt ttaagtgggc
    31561 aacgcgcagg agcggaacat ggccctgcga cggtagcaat tggttcgttg attgcagcgt
    31621 taattttcgt gccaattgga gcgcttcagg ctggtgaagc actctggcac tggtcggtta
    31681 ttccattggg tctggctgtc gctattctct cgaccgctct gccttattcg ctggaaatga
    31741 ttgccctcac ccgtttgcca acacggacat ttggtacgct gatgagcatg gaaccggcgc
    31801 tggctgccgt ttccgggatg attttcctcg gagaaacact gacacccata cagctgctgg
    31861 cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct gacagtacgc aaagagagca
    31921 aaataaaaga attagacatt aattaaattt acatttctgt atggttatgc ataaccatac
    31981 agaatttctc gctacttttc ctctacac
//
LOCUS       DAJTUN010000035        31837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTUN010000035 DAJTUN010000000
VERSION     DAJTUN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.035"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..972
                     /locus_tag="OO470_001534"
     CDS             <1..972
                     /locus_tag="OO470_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6250824.1"
                     /translation="YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAE
                     PMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSR
                     VVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIF
                     VYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTD
                     DMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIA
                     DFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            969..1934
                     /gene="yggF"
                     /locus_tag="OO470_001535"
     CDS             969..1934
                     /gene="yggF"
                     /locus_tag="OO470_001535"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6250825.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            1956..2465
                     /gene="fumE"
                     /locus_tag="OO470_001536"
     CDS             1956..2465
                     /gene="fumE"
                     /locus_tag="OO470_001536"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6250826.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2462..3175
                     /locus_tag="OO470_001537"
     CDS             2462..3175
                     /locus_tag="OO470_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6250827.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3147..3829)
                     /locus_tag="OO470_001538"
                     /pseudo
     CDS             complement(3147..3829)
                     /locus_tag="OO470_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(3823..4500)
                     /locus_tag="OO470_001539"
     CDS             complement(3823..4500)
                     /locus_tag="OO470_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6250828.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4488..4871)
                     /locus_tag="OO470_001540"
     CDS             complement(4488..4871)
                     /locus_tag="OO470_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6250829.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4887..5195)
                     /locus_tag="OO470_001541"
     CDS             complement(4887..5195)
                     /locus_tag="OO470_001541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250830.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5196..5774)
                     /locus_tag="OO470_001542"
     CDS             complement(5196..5774)
                     /locus_tag="OO470_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6250831.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5797..6228)
                     /locus_tag="OO470_001543"
                     /pseudo
     CDS             complement(5797..6228)
                     /locus_tag="OO470_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6600..7619
                     /gene="epd"
                     /locus_tag="OO470_001544"
     CDS             6600..7619
                     /gene="epd"
                     /locus_tag="OO470_001544"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6250832.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7669..8832
                     /gene="pgk"
                     /locus_tag="OO470_001545"
     CDS             7669..8832
                     /gene="pgk"
                     /locus_tag="OO470_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6250833.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9047..10126
                     /gene="fbaA"
                     /locus_tag="OO470_001546"
     CDS             9047..10126
                     /gene="fbaA"
                     /locus_tag="OO470_001546"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6250834.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10317..11177
                     /gene="mscS"
                     /locus_tag="OO470_001547"
     CDS             10317..11177
                     /gene="mscS"
                     /locus_tag="OO470_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6250835.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11316..11951
                     /gene="argO"
                     /locus_tag="OO470_001548"
     CDS             11316..11951
                     /gene="argO"
                     /locus_tag="OO470_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6250836.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12044..12784
                     /locus_tag="OO470_001549"
     CDS             12044..12784
                     /locus_tag="OO470_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6250837.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12951..13901
                     /locus_tag="OO470_001550"
     CDS             12951..13901
                     /locus_tag="OO470_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6250838.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13843..15321)
                     /gene="scpC"
                     /locus_tag="OO470_001551"
     CDS             complement(13843..15321)
                     /gene="scpC"
                     /locus_tag="OO470_001551"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6250839.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15345..16121)
                     /gene="scpB"
                     /locus_tag="OO470_001552"
     CDS             complement(15345..16121)
                     /gene="scpB"
                     /locus_tag="OO470_001552"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6250840.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16132..17127)
                     /gene="meaB"
                     /locus_tag="OO470_001553"
     CDS             complement(16132..17127)
                     /gene="meaB"
                     /locus_tag="OO470_001553"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6250841.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17120..19264)
                     /gene="scpA"
                     /locus_tag="OO470_001554"
     CDS             complement(17120..19264)
                     /gene="scpA"
                     /locus_tag="OO470_001554"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6250842.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19468..20361)
                     /gene="argP"
                     /locus_tag="OO470_001555"
     CDS             complement(19468..20361)
                     /gene="argP"
                     /locus_tag="OO470_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6250843.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20744..21403
                     /gene="rpiA"
                     /locus_tag="OO470_001556"
     CDS             20744..21403
                     /gene="rpiA"
                     /locus_tag="OO470_001556"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6250844.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21659..22891
                     /gene="serA"
                     /locus_tag="OO470_001557"
     CDS             21659..22891
                     /gene="serA"
                     /locus_tag="OO470_001557"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6250845.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23120..23179
                     /gene="ibsC"
                     /locus_tag="OO470_001558"
     CDS             23120..23179
                     /gene="ibsC"
                     /locus_tag="OO470_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6250846.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23512..23571
                     /locus_tag="OO470_001559"
     CDS             23512..23571
                     /locus_tag="OO470_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6250847.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23672..24274)
                     /locus_tag="OO470_001560"
     CDS             complement(23672..24274)
                     /locus_tag="OO470_001560"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6250848.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24295..24478)
                     /gene="ssrS"
                     /locus_tag="OO470_001561"
     ncRNA           complement(24295..24478)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO470_001561"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24520..24849)
                     /gene="zapA"
                     /locus_tag="OO470_001562"
     CDS             complement(24520..24849)
                     /gene="zapA"
                     /locus_tag="OO470_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6250849.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25017..25595
                     /gene="ygfB"
                     /locus_tag="OO470_001563"
     CDS             25017..25595
                     /gene="ygfB"
                     /locus_tag="OO470_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6250850.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25621..26946
                     /gene="pepP"
                     /locus_tag="OO470_001564"
     CDS             25621..26946
                     /gene="pepP"
                     /locus_tag="OO470_001564"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6250851.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            26943..28121
                     /gene="ubiH"
                     /locus_tag="OO470_001565"
                     /gene_synonym="visB"
     CDS             26943..28121
                     /gene="ubiH"
                     /locus_tag="OO470_001565"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6250852.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28144..29346
                     /gene="ubiI"
                     /locus_tag="OO470_001566"
                     /gene_synonym="visC"
     CDS             28144..29346
                     /gene="ubiI"
                     /locus_tag="OO470_001566"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6250853.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29420..29503)
                     /locus_tag="OO470_001567"
     CDS             complement(29420..29503)
                     /locus_tag="OO470_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250854.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29794..30888
                     /gene="gcvT"
                     /locus_tag="OO470_001568"
     CDS             29794..30888
                     /gene="gcvT"
                     /locus_tag="OO470_001568"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6250855.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            30912..31301
                     /gene="gcvH"
                     /locus_tag="OO470_001569"
     CDS             30912..31301
                     /gene="gcvH"
                     /locus_tag="OO470_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6250856.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31420..>31837
                     /locus_tag="OO470_001570"
     CDS             31420..>31837
                     /locus_tag="OO470_001570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HCR6250857.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTG"
BASE COUNT         7597 a         7959 c         8599 g         7682 t
ORIGIN      
        1 tattcagcgg gctacagcta cgaatatttt ggcggcaatg ccacttatat gattattccc
       61 gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg
      121 tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta tcacaccacg
      181 caatatgttt atgagcatcg catgggcgtc aaacctggcg gcaatattgc actgctggcg
      241 tgtgcgggtc cgatgggcat tggcgctatc gattacgcca ttaacggtgg catacaaccg
      301 tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca gaagctgctg
      361 ccggtggatc tggcggccag taaaggcatt gagctagtgt atgtgaatac caaagggatg
      421 agcgatccta tccagatgct gcgggcgctg acaggagatg tcgggttcga tgacattttt
      481 gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg aattactggc ggaagatggc
      541 tgtctgaact tctttgccgg gctgacggat aaaaacttca aagtgccgtt taatttctac
      601 aacgtccatt acaacagcac gcacgtagtc ggtacatccg gcggttcaac ggacgacatg
      661 aaagaggcga ttgcccttag cgccacagga cagttacagc cgtcgtttat ggtgacccat
      721 attggtggcc tggatgcggt gccagaaacc gtgctcaatc tgccggatat ccctggcggt
      781 aaaaaactca tttataacgg cgtgaccatg ccgctcactg ccattgccga ttttgccgaa
      841 aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc tggttgagga aacgcacggc
      901 atctggaatg aacaggccga gaaatatctg ctggcacaat ttggcgttga tatcggggag
      961 gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt tacggaacag gcagcgttgg
     1021 ctgcctggcc gcacaccgga tgtggcgaca aaaataaaat tgatggcctg gcggtcaccg
     1081 cgatgcgcca ggcattaaac gacgtcgctt ttcgtggacg agtggttatc ggcgaaggag
     1141 aaattgacca tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc gatgggccag
     1201 aagtcgatat cgcggttgat cccattgaag gcacgcggat ggtggcgatg gggcagagca
     1261 atgcgctggc ggtcatggct ttcgctcctc gagatagcct gttacatgcg cccgatatgt
     1321 atatgaaaaa gctggttgtt aatcgactgg ctgccggggc gattgatctg tctttgcctc
     1381 tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa accgctggac aagctgcgca
     1441 tggttacgct ggataaaccg cgtctgagtg ccgcgattga agaagcaaca cagctgggcg
     1501 tgaaggtttt tgccctgccg gatggcgatg tcgccgccag cgtgctgacc tgctggcagg
     1561 ataatcctta cgatgtgatg tacaccatcg gcggtgcgcc agaaggcgtg atttctgcct
     1621 gtgccgttaa agcgttaggc ggggatatgc aggccgagct gattgacttt tgtcaggcta
     1681 aaggggatta cacggaaaat cggcagattg cagagcagga gcgcaagcgt tgtaaggcaa
     1741 tgggcgttga cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg aacgatatcc
     1801 tctttagcgc tacgggcgtg acgggcggcg agttggtgaa cggtatccaa cagacggcga
     1861 atggggtgcg gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt aatataatag
     1921 actccctgca ttgatggtga tctatcggaa gaccaatggc gacgctgaca gaagatgatg
     1981 tgcttgagca actggatgca caggacaatt tattttcatt tatgaaaact gcgcattcta
     2041 ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac gatgaagaga
     2101 tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac gatattgatg
     2161 ttgcgctgcg tttgatttat gcgctgggga aaatggataa atggctgtat gccgatatca
     2221 cgcacttttc ccagtactgg cattacctga acgaacagga tgaaacgccc ggatttgccg
     2281 atgatataac ctgggatttt atcagcaatg tcaacagtat aactcgcaat gcgacgctct
     2341 acgatgcgtt aaaagcgatg aagttcgccg atttcgccgt ctggtcagag gcgcgtttta
     2401 gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag gaattaacgc
     2461 cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc atgatgaaga
     2521 gatagaacgc gtgcataaac tgttgctgcg tatgctggcg gccttacaga cggtaaatcc
     2581 gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat ctaccctaac
     2641 caccttctgg gaatatctcg cgcaacaaga cccggagctg cccgctattc aggcgctccc
     2701 gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc gccccttcaa
     2761 aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc aggtggtgga
     2821 aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg aagatgcgtt
     2881 gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg acgacgagaa
     2941 gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc cagctgctgc
     3001 gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg cagatgcaga
     3061 agccttttat gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag agagtctgcc
     3121 tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg attaacgttc
     3181 ctgattacac catcttccag ccgcaccaca cgagagaaat gacgccgggt aaatgcggcg
     3241 tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag ccagctttca
     3301 aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag taatagtggc
     3361 ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcact atgcagatcc
     3421 aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc atgggtacgt
     3481 tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat ctcgtcagcg
     3541 acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gaacagtacg ccaacctttg
     3601 ccgcgcgctg gcggtttttc aggtttttca tcgcctgttg ttgcagcata accgtaccgg
     3661 ctgtgggcgt aagaagacct gccattactc gtaacagcgt cgatttccct gcgccattgt
     3721 caccggtcag cgccaaccat tcaccttgtt tgagttgcag agaaatgtcg caaagacaat
     3781 ccgttgccgc gccaggccag cgataagata tcttatttaa cgttaacata gtggctggag
     3841 cgctccgttt tgcaattgcc acgcttgctg gcagtagttc aggaaaggtg tgcgatgacg
     3901 ttcaaaaaga ataatcagcg aaccacgttc aaacgcccag tgttgtaagc gctgcaacag
     3961 catttcgctg gcctgcggcg tcaggcggct aaaggcttca tcaagaatta agagctttgg
     4021 ctgcatcgcg atggcgcagg cgatgatcac gcgctgtgtc tctccaccgg agagtgttgc
     4081 cgggtgacga tggcgcaacg gctggcactc ggtcagagtc agggcggcat caatccgcgc
     4141 cataatctct gcttcagcaa gacacagatt ttctggccca aaggcaactt cttcttccac
     4201 gctaaaggta cagccggaga gctgcaaata gggcgattgc tggacgagct ggatggtggc
     4261 ggattgttca ttaagcggga gatgcccaat gggcgtaccg aggagcgtcc ccgtgcctgt
     4321 tatctctctt ggcagaaagt ccggatacca gcccgccatc agctgtgcca gcgtactttt
     4381 gccactgccg ttgtcaccaa agatggcgac catccccggt ttgacatagt gaaagtcata
     4441 acagaacgga ggatgcgttg agtgtgtcgg gcaataacgg aactgttcta acgtaaccat
     4501 atccacgctc cgaattctgt cagcatcagc aagatcatgg tgtaacgcgc tacgcgttgt
     4561 agggtgctat cggctggtgc ccacaacgtt gttcgtcggt tgtggatccg aaatgcccgc
     4621 atatccagcg ccgcaccacg gacagccaga tcgttaagcg cattgtgtgt tagcggaata
     4681 attaacgcgg gcatagcacg taaacgctga taccagccct catccagcgg cacgccgcgg
     4741 gcacgctgcg cttcatgaat aatcgctaat tgccgtttta actgctcaac gaccaataac
     4801 ggcccggcaa acagataagc gacgcctggc ggcaggcgag aagcaaacag agcgcgaata
     4861 aaacattgca cggggacgta ctgcattcac aactgtgagg ttgaaacaat cgccagaatc
     4921 agcaaccaga gcgtaatagc atgcgaccac cgttctggcg agcgtggcgt gccgcttagc
     4981 cattctggta gccagccacc gtgtaccagc cacagcccgg ctcccagcga gaacatcagc
     5041 cagacgacat attttgcccg ccgccgcgtg gcctttagcg caataaggca gaaaaaggtc
     5101 gccgcgctat agatcggcaa aatggtctgc gcgggcagga taagcgtggt gcaggccgcc
     5161 aatgcccaca gtgttaatga ggtaaatgga tgcatttagc gcacggcagc cattgccgga
     5221 aaatggcgcg tggtgcgaat cggcagctgg cgcagtaaaa gccagacaat caccgctgta
     5281 agaattttat cgaccaggtt cgcgcctatc accgtaatcg ccaccgactc caccaggttt
     5341 tgccccatcg agtgcatcca ggcaacgaac aaatcggctc cgctaccggt gacaccgccg
     5401 aacagcgcag tacgcagcgg tacagcgacg accgttaccg caagcgtaat aatcacgccg
     5461 ctgacaacga cttttggcag ggtgcgaaac caacccgctc gcgccagcca accggagacc
     5521 aggccgatga ccatcgcgac gggtgcaaat gcggcggcga tcgggtcggt cagcaatccc
     5581 cagagtaaat tggtcagtaa gccggtcagc attccgatca ccggaccaag cagtaccgcg
     5641 ctaataagtg tgccgatgga gtcgagaaat ataggtaatt tgaccatgct ggccagttgc
     5701 ccgccgatca tattgatggc gatagatata acgatcaaca ccagtgcctg actggaaaag
     5761 tggctacgcg ccataagaaa acctctttga aagtagttat ttaacggggg aaatatgcaa
     5821 ctcgtcgtaa ccagaaagcg cgcaaggttg gcggctaaac gccagttcct caaggctacc
     5881 gatcaccagt tcaagcgtgg tccgcacggt acagccgggc atcatatgac cgccaagcat
     5941 cgtgccgtgt ggatcggaaa cgcagagatg aagatgctca ccactctgtt ccagcgtgcc
     6001 gtttagtgag atcacttcga atgtaccgtt caggagcgtg gtgccttctt gcccggcata
     6061 gcgcaaggcg acgtcagtca ggctaccggt acagcctgca atctaggcgg catgaagttg
     6121 atgttgctgt gcaaaagcgt gtagctggga aaaaacttcc tgcccgggga ggaggcgcag
     6181 tgcgtaaaag cgtgcggagg aagaatatga gtagggcaat gtcatcattg gagtactccg
     6241 aaactcggac gtattgcgga taatatcgac gctgcatcag tcatattctg atataagtca
     6301 gggtttgtga cggcacaacg aaaagatttt gctacgctta ttcgtgattc tgcaaagtgg
     6361 aataaattcc acaggggcgg ggctgaaaac gtgacgtaag tcagataatt gctttctttg
     6421 actggacaat cattcctttt attccacgtt tcgcttatcc tagctgaagc gtttcagtcg
     6481 attaaatgtt cgacaattaa ccaatcagtc gcagtttgcg acaggtaagg tttccccgga
     6541 cgatttgctg gattactctg tctggcaaat ttgctcaggg aaaaccttgc aggagatcta
     6601 tgaccgtacg cgtagcgata aatggcttcg gtcgcatcgg gcgtaatgtg gttcgtgctt
     6661 tgtatgaatc cggacgccgg gcggaaatta ccgtggtggc aatcaacgaa ctggcggatg
     6721 ctgcgggcat ggcgcatttg ttgaaatatg acaccagcca tggccgtttt gcatgggaag
     6781 tacgacagga acgcgatcaa ctttttgttg gtgatgacgc catccgcgta ttgcatgaac
     6841 gttcactgca atcgctcccc tggcgtgaac ttggcgttga tgtagtcctc gactgcaccg
     6901 gcgtatatgg ctcccgcgag catggcgaag cgcatattgc cgccggggcc aaaaaagtgc
     6961 tcttttcaca tcctggcagt aacgatctcg acgcgaccgt tgtttacggc gtcaatcagg
     7021 atcaacttcg tgcggaacac cgcatcgttt ctaacgcttc ctgtaccacg aattgcataa
     7081 ttcccgtcat caaattgtta gatgatgcgt acggtattga gtccggcact gtgaccacaa
     7141 ttcactccgc catgcacgat caacaggtta ttgatgcata ccatcctgac ctgcgtcgca
     7201 cccgggcagc cagccagtcg atcattccgg tcgatactaa actggccgct ggtatcacac
     7261 gattttttcc gcaatttaac gatcgctttg aagcgattgc ggtacgtgtg ccaaccataa
     7321 atgtgacggc aatcgattta agcgtgacgg tgaagaaacc tgtaaaagcc aatgaagtca
     7381 acctgttgct gcaaaaagca gcacaaggtg catttcatgg tatagttgac tatacggaat
     7441 tgccgttggt ctctgtagat tttaaccacg atccgcacag tgccattgtc gatggcaccc
     7501 aaacccgggt cagtggcgca cacctgatca aaacgttggt ctggtgcgat aacgaatggg
     7561 gctttgctaa ccgaatgctc gacacgacgt tagctatggc tactgttgct ttcaggtaag
     7621 acgcaagcag cgtctgcaaa acttttagaa tcaacgagag gattcaccat gtctgtaatt
     7681 aagatgaccg atctggatct tgctgggaaa cgtgtattta tccgtgcgga tctgaacgta
     7741 ccagtaaaag acgggaaagt aaccagcgac gcgcgtatcc gtgcttctct gccgaccatc
     7801 gaactggccc tgaaacaagg cgcaaaagtg atggtaactt cccacctggg tcgtcctacc
     7861 gaaggcgagt acaacgaaga attctctctg ctgccggttg ttaactacct gaaagacaaa
     7921 ctgtctaacc cggttcgtct ggttaaagat tacctcgacg gcgttgacgt tgctgaaggt
     7981 gaactggttg ttctggaaaa cgttcgcttc aacaaaggcg agaagaaaga cgacgaaacc
     8041 ctgtccaaaa aatacgctgc actgtgtgac gtgttcgtaa tggacgcatt cggtactgct
     8101 caccgcgcgc aggcttctac tcacggtatc ggtaaattcg ctgacgttgc gtgcgcaggc
     8161 ccgctgctgg cagctgaact ggacgcgctg ggtaaagcac tgaaagaacc tgctcgcccg
     8221 atggtggcta tcgttggtgg ttctaaagta tctaccaaac tgaccgttct ggactccctg
     8281 tctaaaatcg ctgaccagct gattgttggt ggtggtatcg ctaacacctt tatcgcggca
     8341 caaggccacg atgtgggtaa atccctgtac gaagctgacc tggttgacga agctaaacgt
     8401 ctgctgacca cctgcaacat cccggttccg tctgatgttc gcgtagcaac cgagttctct
     8461 gaaactgcac cggctaccct gaaatctgtt aacgatgtga aagctgacga gcagatcctg
     8521 gatatcggtg atgcttccgc tcaggaactg gctgaaatcc tgaagaatgc gaaaaccatt
     8581 ctgtggaacg gtccggttgg cgtgttcgaa ttcccgaact tccgcaaagg tactgaaatc
     8641 gtggctaacg ctatcgcaga cagcgaagcg ttctccatcg ctggcggcgg cgacactctg
     8701 gcagcaatcg acctgttcgg cattgctgac aaaatctcct acatctccac tggcggcggc
     8761 gcattcctcg aattcgtgga aggtaaagta ctgcctgcag tagcgatgct cgaagagcgc
     8821 gctaagaagt aaaaaatcac agggcaggga aacctgcact tgtttcagcg cgcttttaga
     8881 gcacgcattt tttcatactc taaataattc gagttgcagg aaggcgacaa gagagtgaat
     8941 ccccaggagc ttacataagt aagtgactgg ggggagcgaa cgcagacgca gcacatgcaa
     9001 cttgaagtat tatgagtata aggcccgacg atacaggaca agagacatgt ctaagatttt
     9061 tgatttcgta aaacctggcg taatcactgg tgatgacgta cagaaagttt tccaggtagc
     9121 aaaagaaaac aacttcgcac tgccagcagt aaactgcgtc ggtactgact ccatcaacgc
     9181 cgtactggaa actgctgcta aagttaaagc gccggttatc gttcagttct ccaacggtgg
     9241 tgcttccttt atcgctggta aaggcgtgaa atctgacgtt ccgcagggtg ctgctatcct
     9301 gggcgcgatc tctggtgcgc atcacgttca ccagatggct gaacattatg gtgttccggt
     9361 tatcctgcac actgaccact gcgcgaagaa actgctgccg tggatcgacg gtctgctgga
     9421 cgcgggtgaa aaacacttcg cagctaccgg taagccgctg ttctcttctc acatgatcga
     9481 cctgtctgaa gaatctctgc aagagaacat cgaaatctgc tctaaatacc tggagcgtat
     9541 gtccaaaatc ggcatgactc tggaaatcga actgggttgc accggtggtg aagaagacgg
     9601 cgtggacaac agccacatgg acgcttctgc actgtacacc cagccggaag acgttgatta
     9661 cgcatacacc gagctgagca aaatcagccc gcgtttcacc atcgctgcgt ccttcggtaa
     9721 cgtacacggt gtttacaagc cgggtaacgt ggttctgact ccgaccatcc tgcgtgattc
     9781 tcaggaatat gtttctaaga aacacaacct gccgcacaac agcctgaact tcgtattcca
     9841 cggtggttcc ggttctactg ctcaggaaat caaagactcc gtaagctacg gcgtagtgaa
     9901 aatgaacatc gataccgata cccaatgggc aacctgggaa ggcgttctga actactacaa
     9961 agcgaacgaa gcttatctgc agggtcagct gggtaacccg aaaggcgaag atcagccgaa
    10021 caagaaatac tacgatccgc gcgtatggct gcgtgccggt cagacttcga tgatcgctcg
    10081 tctggaaaaa gcattccagg aactgaacgc gatcgacgtt ctgtaagtca ttcctttctg
    10141 cttatctcaa ggcccgctct gcgggtcttt ttttcgccaa aagcaaggtg attcagatga
    10201 gaaatctgtg atctatttgg caaaattatg ctttattgtt tacccttgtc agactgcccg
    10261 tcataaggcg gcggagtgta tttctccatt ttgagtcagt tgaaaaggaa tattgaatgg
    10321 aagatttgaa tgttgtcgat agcataaacg gcgcgggaag ctggctggta gctaaccagg
    10381 cgctgctgct aagttatgca gtaaacatcg tggcggcact cgcgatcatc atcgttggtt
    10441 tgattatcgc gcggatgatt tccaacgcgg tgaatcgcct gatgatctcc cgtaaaatcg
    10501 atgccactgt tgctgatttt ctttctgcat tagtccgtta cggtattatc gcctttacgc
    10561 taatcgctgc cctgggacgc gtgggtgtac aaacagcgtc agtcattgct gtactcggtg
    10621 ccgcaggctt agctgttggt ctggctttgc aggggtcact ttctaacctg gccgctggcg
    10681 tgttacttgt catgttccgc ccgttccgtg ccggagaata tgttgacctc ggcggcgtag
    10741 ccggtactgt gctgagtgtg cagattttct ccaccaccat gcgtactgca gacggtaaaa
    10801 ttatcgttat tccgaacggt aaaattattg ccggaaatat tattaacttc tcccgcgagc
    10861 cagttcgccg taacgaattt attattggcg tggcgtatga ttccgatatc gatcaggtta
    10921 agcagatcct gaccaatatt atccagtctg aagatcgcat tttgaaagat cgcgaaatga
    10981 ctgtgcgcct gaacgaactt ggtgcatcgt cgattaattt cgtggtccgc gtctggagca
    11041 acagcggcga tctgcaaaac gtgtactggg atgtgctgga gcgtattaaa cgtgaatttg
    11101 atgccgccgg tatcagcttc ccgtacccgc aaatggatgt gaactttaag cgggtgaaag
    11161 aagacaaagc tgcgtaatca acgctgaggc cagataatac tctctggcct ctctcttatt
    11221 agttattctg attgccaatt aatattatca atttccgcta ataacaatcc cgcgatatag
    11281 tctctgcatc agatacttaa ttcggaatat ccaacgtgtt ttcttattac tttcaaggtc
    11341 ttgctcttgg ggcagctatg attctgccgc tcggcccaca aaatgctttt gtgatgaatc
    11401 agggcattcg tcgtcagtac cacattatga ttgccttact ttgcgctatc agcgatttgg
    11461 tcctgatttg cgcggggatt tttggtggta gcgcgttatt gatgcagtcg ccgtggttgc
    11521 tagcgctggt cacctggggc ggcgtagtct tcttgctctg gtatggtttt ggcgctttta
    11581 aaacagcgat gagtagtaat attgagttag ctagtgccga agtcctgaag caaggcagat
    11641 ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt tacctggata
    11701 cttttgttgt actgggcagc cttggcgggc agcttgacgt cgaaccaaaa cgttggtttg
    11761 cgctcgggac aattagcgcc tctttcctgt ggttctttgg tctggctatt ctcgcagcct
    11821 ggctggcacc gcgcctgcgc acggcaaaat cacagcgcat tatcaatctg gtagtgggat
    11881 gtgtcatgtg gtttatagcc ttgcagctgg cgagagacgg tattgctcat gcacaagcct
    11941 tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct tgcctccaga
    12001 ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt tcaaagttat
    12061 cgccctggcg gcattaatgg gtattagcgg gatggcagcg caggctaacg aattgccgga
    12121 tggaccgcat attgtcacct ccggtacggc aagtgtggat gcggtgccag acattgccac
    12181 tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct acagccaaga aacaggcaga
    12241 tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga aaaaagatat
    12301 cagctcagcg aatttacgca cccagccaga ttatgactat caggatggta aaagtatcct
    12361 gaaaggctac cgagctgtga gaacggtgga agtcacgctc cgtcagttag acaaactgaa
    12421 ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttctg tgtcgctggg
    12481 cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg ataacgcgat
    12541 tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg tatatagcgt
    12601 gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga aagccgatgc
    12661 cgcgccggtg tctgcccagg aaacttacga gcaggccgct attcagtttg atgatcaggt
    12721 cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac ctgcagcaca
    12781 ataatacttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata ttgcctcctt
    12841 tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc ggtttcatca
    12901 aggataggct gactgaaacg gttaagcgga caaggatgcg gggagggggg atggacattt
    12961 ttatttcgaa aaagatgcgc aatttcattt tattagcgca aaccaataat attgcccggg
    13021 cggcagaaaa aatccatatg acggcttcgc catttggtaa aagcattgcc gcgctggaag
    13081 aacaaattgg ctatacgcta tttacccgca aagataataa tatcagcctc aataaagccg
    13141 gtcaggaact gtatcaaaaa ctgttcccgg tttatcaacg actttctgcg attgataatg
    13201 aaatccataa ctcagggcgt cgttcacgag aaattgtgat tggcatagat aatacttatc
    13261 cgacaattat tttcgatcag ttgattagtc tcggagataa gtatgaaggc gtgactgccc
    13321 agccagttga gttcagcgaa aacggcgtta tcgataatct ttttgaccgt cagttagatt
    13381 ttattatctc cccacagcat gtatctgcac gagttcagga actggaaaac ctgactatca
    13441 gtgaattacc accgctgcga ttgggctttc tcgtttctcg ccgttatgaa gagcgtcaag
    13501 agcaagagct gcttcaggag ctgccctggc tacaaatgcg ctttcaaaac cgggctaact
    13561 tcgaagcaat gattgatgcg aatatgcgac cttgtgggat caatcccacc attatttatc
    13621 gtccctacag ttttatggcc aaaatcagcg ccgtggagcg cggacatttt ctgacggtga
    13681 tcccgcattt tgcctggcga ctggtgaatc cggcaacgct gaaatatttt gatgcgccgc
    13741 acaggccgat gtatatgcag gaatatcttt attcaatcag aaatcatcgt tataccgcca
    13801 cgatcttcag catattgctg aagatcgtga cgggacaaac cattaaccca gcatcgagcc
    13861 ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt gaatatgtcc
    13921 gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat gtgcacaatt
    13981 atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg cgatcccttg
    14041 ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc acattggcac
    14101 gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg acagatacgc
    14161 gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc cggctacgtg
    14221 cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag cgatgacgcc
    14281 cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca caatgcggct
    14341 ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga ttgtcaggct
    14401 ggaggcgctg gccccgctga ttttgccggt ttccagtaaa tgcaccaccg attcctgtag
    14461 cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac gtgccattac
    14521 cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg gaatacgccc
    14581 atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct ggcacatggg
    14641 attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga cggcgacaat
    14701 ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat ggaagatcga
    14761 cacgctattg cgccgcggtg gcgcgccagg aatcacaata tctgccagtt ctgcaacgcg
    14821 cggatcgtga tagtgattaa gttcaatgat cactttcttc gctcgcagca gccaggtcgg
    14881 cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg ccgatgcttc
    14941 aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg ccacttcgct
    15001 caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta aactggacga
    15061 tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat cgtcagcggc
    15121 ggcgctgatt gacgcccccg tcagcaggcg aatttgatac ggctttttgg cctcatgctg
    15181 ttcgttagct ctgcgggcaa tcgcggcggg tagggctttc ggcgaacccg ctggggtaaa
    15241 gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat cggcggtcat
    15301 ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg accaacgaaa
    15361 ttaggtttac gtttttcgag gaaagcgttc atcccttcct ggtaatcttc gctgtcataa
    15421 accgcgcggc gcatcccctg aatacgttca aattcatcgg agttcatggt gtgtgcttcg
    15481 cccagtacac gcagctcttc tttgataacg gcaatggcta acggcgcttt ctcagagatg
    15541 tggtgcgcca tttgtaaggt gaaatcttcc acttccacaa catggttgag gatgccgaca
    15601 gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg
    15661 aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac gccgaggttt
    15721 acaggcgtca ttgagaaggt tgaggtactg gcggcgatga tcagatcgga actcatgatc
    15781 atttcaaatg cgccacccca aacactacct tccaccatcg aaatgatcgg tttcgggaat
    15841 ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg
    15901 ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc acttggcgcc
    15961 cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg cataagatca
    16021 tcaataaaga ctttacttaa ggcattaagt tttcgtccat agttaaactc aatgaccgcc
    16081 actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc tttaatcaaa
    16141 atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt
    16201 gtttttgacc gctaaaagcg tctggtggta atagcgatcg aaatcttcat tcgcgaacag
    16261 gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt gttgttgccg
    16321 cacttgttgt aaacgaccac tggcagttag cgcggttttg aagtcgatga tggcgtgcca
    16381 gatctcatcg attccacgtt tttccagtgc gctacaagtc agaccccgtg gctgccattc
    16441 gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg caatggcgac
    16501 attggtatgg ttatcgccat cgtctttgtt gataacgagc agatcagcca cttccatcag
    16561 ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg agataaaaca
    16621 gtccaccatg cgggcgactt ccgtttccga ctgcgcgacg ccaaccgttt cgacaatcac
    16681 tacgtcataa cccgccgctt cgcataacag cattaattcc cgtgctcgct gactggcacc
    16741 gcccagatga ccggaggagg gtaccgggcg aataaacgcc gcctcggcac gcgccaggtc
    16801 attcatgcgg gttttatccc cgagaatgct accgccagtg accgggctgc tgggatcgac
    16861 cgcaataaca gcaaccttta atccctcacg aatcaacaac atgccaaagg cttcaagaaa
    16921 ggtacttttc cccgcgccgg gtgtgccggt aacgcccagt cgcagggcgt taccacagaa
    16981 cggcataata gcgtcaagca gctgtgtact tagcgcctga tgacgtgggt gacggctttc
    17041 caccagcgtc atggcttggg cgagcgtggc acgctcaccc tgacgtaagc ggcgaatact
    17101 ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt acgtcgcgca
    17161 cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc tcttgcagga
    17221 aggcgtaatc ctgcggcgga atgacgccac ccgcgaccac gcagatatct tcgcgtcccc
    17281 attttttcag cgcttcgacc agttccggga tcagcgtttt atgaccggca gccagtgagg
    17341 atgcgcccac tacgtgaacg tcgttttcta cggccaggcg ggcgatctct tcaggtgtag
    17401 agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg gcgatcactt
    17461 tcgcgccgcg atcgtgtcca tcctggccca tcttagcgat cagaatgcgc gggcgacgac
    17521 cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg gaggccgatt
    17581 tctcagactg atgatagctt tgcgcaatca cgccggtaac acactggctt ggcaccagat
    17641 aacggtcgaa agcgacttcc agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag
    17701 cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt
    17761 tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa
    17821 tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg tcttcgtgat
    17881 ccagtttgta cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt
    17941 cgcgcgctga ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc gctttcgcca
    18001 tgccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg atttgatcgg
    18061 tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgtggcag agttctgatt
    18121 cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac
    18181 caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc gtcgcagcca
    18241 gtgcttcaat ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc aggctccagc
    18301 ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg atcctgtgcg ccaaatccac
    18361 tgaccgcttc gctccataaa taacgtgccg cacgcaacat ggcgacgttc ataaacagat
    18421 ccatgccgat accgaagaat aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg
    18481 cagagattgc tgctttgatg tactcaatcc catcggcgat cgtaaatgct acctgctgca
    18541 cgcagttggc acccgcttcc cccatgtggt aaccgctgat actgatggta ttaaatcgcg
    18601 gcatgttgcc ggaacaccag gcgatgatgt cggcgataat gcgcattgac ggttttggtg
    18661 ggtaaatata ggtgttgcgg cagaggtact ctttgagaat atcgttttga atggtgccgg
    18721 tcagtttatc aggtgtaaca ccttgctctt ctgcggcgac gatataaaac gccagtactg
    18781 gtagcactgc gccattcatg gtcatcgaaa ccgacatttt atccagcggg atctggtcga
    18841 acaggacttt catatcttcc acggtgtcga tagcgacgcc cgctttgccg acgtcacccg
    18901 ccacgcgtgg gttatcggag tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa
    18961 gacctttttg cccggcggcc aggttacggc gataaaaagc gttggactct tttgctgttg
    19021 aaaaaccagc atactgacgg atggtccacg gttgggcggt atacatagtg gcacgcgggc
    19081 cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac ctccagatta tcgagatcgg
    19141 cttcggtata cagcggcttg atggcgatcc cttccgcggt ttgctgaacc agcgagtcga
    19201 cagttttctc ccgacggctc aattccttgt tggcaagctg ttgccactcc tgctcgttag
    19261 acattatttg ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt
    19321 ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt gccgcctgag
    19381 atttgtctat tttgtgatgt gattcaagga atacaaaaag aagtgaggac aacgcctgat
    19441 atgtgctatc aggcattatt tgatggatta atcctgacga aggactttgt gaccataatc
    19501 gagtaacgca tcagtgactt tacgcatcat gcggctttca ggagcaaagc ggtgccagta
    19561 gagcatccgt cgttgaaata gcccaggcgt taagtcaatc agttcaccgc tggccagctc
    19621 tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag caagttgtac
    19681 gaacgcttct gaagaattaa cgatatggca gggtacgctg ccaggaggaa gatcgaagtt
    19741 ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca aacgcgacca ctggcgcttt
    19801 caataatgcc gaacgcgtta cgccgttagg aaaatatttt tcggcaaacg gttttgagct
    19861 gacgaacaga tagtcgagcg caccgagttt atcgacaaga caactcggca gcgcctgatg
    19921 ttgaatactc accgcgccga ccacttcgcc gcggcgcaga cgttcctgag tacgggtttc
    19981 atcttctacc tgcaagttga ggcggatagg cgaatcagcc aacacaggag ccagtgccgg
    20041 aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga
    20101 accggtttgt tcatcgccca gccactcttc ttccagcaac tccacctggc gcagcagtgc
    20161 cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca acagcggctg
    20221 cccgaacata ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg taatgcacag
    20281 cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg cctgtaatgt
    20341 tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact
    20401 atgacacaat tttgtgtcag gttgcatata ccgaagccgt cggcgggtaa catttgtatc
    20461 aatggttaaa cgcgcagctt aaaagatcag ggatttgcgc tattgcattt cgcttagcga
    20521 cttcgcgccc tggttatgtt gaatggcgtg gcgttattgc ctctgttcgc accgtggagc
    20581 ggcataccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc cgccattgcg
    20641 cgatatttta tggatgagtt aaccacccag cttgccaacg gggtctgaat cgcttttttt
    20701 gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc aggatgaatt
    20761 gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggtacca ttgttggtgt
    20821 aggtacaggt tccaccgccg cacactttat tgacgcgctc ggcacgatga aaggccagat
    20881 tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca
    20941 cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg cagatgaaat
    21001 caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat
    21061 tgcttcggtt gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg ttgatattct
    21121 gggtaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag tggcgcgcca
    21181 gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg ataatggcaa
    21241 cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg aaaacgccat
    21301 aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg cggacgttgc
    21361 gctgattggc acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc
    21421 ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgctt ttaccaggca
    21481 attgtcgatt gctctaaata aatcctctaa accagcatat tcatccaaga attacctttg
    21541 cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat tattttttga
    21601 tatgttgaaa ggcggatgca aatccgcaca caacatttca aaagacagga ttgggtaaat
    21661 ggcaaaggta tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca
    21721 aaaggcgctg gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc
    21781 gctggatgat gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc
    21841 ccgtacccat ctgactgaag acgtgatcaa cgccgcagaa aaactggtcg ctattggctg
    21901 tttctgtatc ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt
    21961 atttaacgca ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct
    22021 gctgctattg cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa
    22081 actggcggcg ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca
    22141 tattggtacg caattgggca ttctggctga atcgctggga atgtatgttt acttttatga
    22201 tattgaaaac aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct
    22261 gaatatgagc gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat
    22321 gggcgcgaaa gagatttcgc taatgaagcc cggctcgctg ctgattaatg cctcgcgcgg
    22381 tactgtggtg gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc
    22441 ggcaatcgac gtattcccga cggaaccggc gaccaatagc gatccattta cctctccact
    22501 gtgtgaattc gacaacgtcc ttctgacgcc acacattggc ggttcgactc aggaagcgca
    22561 ggagaatatc ggcttggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac
    22621 gctctctgcg gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat
    22681 gcacatccac gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca
    22741 gggcgtcaac atcgccgcgc aatatctaca aacttccgcc cagatgggtt atgtagttat
    22801 tgatattgaa gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc
    22861 gggtaccatt cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg
    22921 tcactgactt gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac
    22981 tttgcgagtc tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat
    23041 ggggatttac gatggcggga cagcatgggg ctgtaacggt aaagccctca ccgaagcgag
    23101 ggcttaaagg agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat
    23161 aagtttcgga gcttattaac agtcagtctc aagggaggag tcatcctccc ttacccttac
    23221 tcactaaatt aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc
    23281 tgaaccgaac tttttgatag ccacttcatc gcccacgttt actgccgtaa tagatccttt
    23341 ttcccaaaaa ttatctctgt tttgcatggg gtttttcgag cacgctcgaa gaaaagattt
    23401 aaaggtagat caatgcgttg tggttattta aagagagctt acgatagctg ggcagcatgg
    23461 ggctgtaacg gtaaagccct caccgaagcg agggcttgaa ggagaagggt tatgatgcga
    23521 cttgccatca tactgattgt actgttactc ataagtttcc cagcttatta acagtcagtc
    23581 acaggggagg agcaatcctc ccttaccctt actcactaaa ttaggttaaa gaatcaacga
    23641 tgtcaatcag ggcgatgcag gtgtatcgcc cttaccactc ccagactttc gacggtgtaa
    23701 ccaccgcagg aagagggata tcccactctt caacggggag tttttccacc aactgacaat
    23761 catgcgcata acccaccggt tgcgttttat agtgctgcca gttttgtaag gtccgatcat
    23821 aaaaaccacc gcccattccc aggcgctgac cgtactcatc aaaggcgacc agcggtgtga
    23881 tcagcacgtc taatcgggaa aggggtagca cgtcacgcac atccagtttt ggctcatgga
    23941 tcttcaacct gttcatcacc agttcgcttt gcggatggta attcaggaac agcaaattac
    24001 cggcactaaa gggatgcaaa actggaagat atacgcgctt accggcgcgc cagagttgtt
    24061 ctattagcgg ctgggtgtcg agttcaccat caaaagagag gaatacagcg accgtatgcg
    24121 ccatcaccac cgggggataa gtcatcatcc gggtagcggc ttgttgaccc atttccagct
    24181 gttgttccgg cgttaacgca cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg
    24241 ataatgtcag tgggagctct ggtagttgcg tcatggctgg tgccagataa gaagggaatc
    24301 tccgagatgc cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc
    24361 gcagttttaa ggcttctcgg acgaaccgag catgctcacc aaccgcggag cgccacattc
    24421 ttgtggtatg aaatatcggc tcaggggact ggcccgcttg cgaacatctc agagaaattt
    24481 tgtcttcacg gttactctac cacagtaaac cgaaaagtgt cattcaaagt tttggttagt
    24541 tttttcggtg atgcgacctt gttcaagtaa cgcttgttct atggtctgct gcagcatccg
    24601 aatacgctgt tccatacttg ccgcgtagtc acgagtcttt gctttttctt gcgctaactc
    24661 atagctgata ttcaatgcgg caatgaagac caactgttca gtatttgtga ctctagtgcg
    24721 ttctttcaga tcttgcaacc gttggttcag atcgtccgct gcctgattca acgcatccct
    24781 ttggtcaggc gggcagttca cacgcagtga acggccaaaa atttggatat cgacgggttg
    24841 tgcagacatg ccaccttcct gctgattgac tgcgctgcct tcgtctttag acccatggtc
    24901 tgcgaagggg cgacactata gctaccctga tgagaagaga caagcccttt tctggtccac
    24961 cagggcccaa agtggtagca tatcatgaat attcctccct ttgacgacga atgcttatgt
    25021 ctatacagaa cgaaatgcct ggttacaacg aaatgaacca gtatctgaac caacaaggga
    25081 cgggtctgac ccctgccgag atgcatggtt taatcagtgg gatgatatgt ggcggtaacg
    25141 atgacagctc atggctgccg ctacttcacg acctgacgaa cgaaggcatg gctttcggtc
    25201 atgagctggc acaggcactg cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg
    25261 acggcttcct ttttcagctt tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg
    25321 ctgatgcact ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga
    25381 agctggacaa agtgaccggc gaaaccggtg aagccatcga cgatctgcgt aacatcgcgc
    25441 agctgggcta cgacgaagac gaagatcagg aagagcttga aatgtcgctt gaagagatca
    25501 tcgaatacgt tcgtgttgcc gcgctgttat gccacgacac ctttactcat ccgcaaccga
    25561 ccgcgccaga agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt
    25621 atgagtgaga tatcccggca agagtttcag cgtcgccgtc aggccctggt ggagcaaatg
    25681 caacccggca gcgccgcgct gatttttgct gcaccagaag taacacgtag cgccgacagc
    25741 gaatacccct atcgtcagaa cagtgacttc tggtacttca ccggctttaa cgaaccggaa
    25801 gcggtgctgg tgctgattaa aagcgatgac actcataacc acagcgttct gtttaaccgc
    25861 gttcgcgacc tgacggcgga gatctggttt ggccgtcgct taggccagga tgccgcgcca
    25921 gagaaactgg gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca actttatcaa
    25981 ctacttaacg gcctggatgt ggtttaccat gcccagggcg aatatgcata tgctgatgaa
    26041 atcgtgaaca gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg
    26101 gcaacgatga tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa
    26161 gagattgccg tactccgccg cgcgggagaa atcaccgcca tggcacatac acgggcgatg
    26221 gaaaaatgcc gtccgggaat gttcgagtac catctggaag gcgaaattca ccacgaattt
    26281 aaccgccacg gtgcgcgcta tccttcctat aacaccattg taggcagcgg tgaaaacggc
    26341 tgcattctgc actacaccga aaacgagagt gaactgcgcg acggtgacct ggtgttgatt
    26401 gacgcgggtt gtgaatacaa aggttacgcg ggcgatatta cccgcacctt cccggtcaac
    26461 ggcaaattca cccaggccca gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc
    26521 agcctgcgcc tgtatcgtcc gggaacttcc attctggaag tcactggtga agtggtgcgc
    26581 atcatggtta gcggcctggt aaaactcggc atcctgaaag gtaatgttga tgaactgatc
    26641 gctcagaacg cccatcgtcc tttctttatg catggcctta gccactggtt aggactggat
    26701 gtccatgacg ttggcgttta tggtcaggat cgctcgcgca ttctggaacc ggggatggta
    26761 ctgaccgtag agcctggtct gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc
    26821 ggtatcggca ttcgcattga agacgacatt gtgattaccg aaaccggcaa cgaaaacctc
    26881 accgccagcg tggtgaaaaa gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag
    26941 caatgagcgt aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg ctggctattt
    27001 cccggttaag tcacggggcg ctgccggtac atttgattga agcgactgcg ccagagtcac
    27061 atgctcatcc gggctttgat ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac
    27121 tggcgcgcat cggcgtctgg caatctctgg ctgattgcgc aaccgccatc actaccgtgc
    27181 atgtcagcga tcgtggtcat gctggattcg tcaccctcgc cgcagaagat taccaactgg
    27241 cggcgctggg gcaggttgtc gaattgcata atgtcgggca acggctgttt gcattgctgc
    27301 gaaaagcacc tggcgtaatg ctgcattgcc ccgatcgcgt agctaacgtt gcccgtactc
    27361 agagtcatgt tgaagtgacg ctggagagcg gcgagatgct gacgggccgc gtgctgatag
    27421 ccgctgatgg cacccattca gcgttagcca ccgcgtgcgg cgttgattgg cagcaggagc
    27481 cttacgaaca actggcagtg attgccaacg ttgctacttc tgttgcgcac gaaggccgcg
    27541 cttttgaacg cttcacgcaa catggcccgc tggcgatgtt gccgatgtct gacggacgct
    27601 gttcgctggt ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg
    27661 agaagttttg ccgtgaactc cagtctgcct ttggctggcg actggggcaa attacccacg
    27721 ccggtaaacg cagtgcttat ccgctggcat taacccgcgc cgccagaccg attacccatc
    27781 gcactgtgct ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg caagggttta
    27841 acctcggtat gcgagatgtc atgagccttg cggaaaccct gactcaggcg catgagcgcg
    27901 gagaagacat cggtgattac ggcatattgt gccgctatca gcagcgtcga cagagcgatc
    27961 gcgaagcaac catcggcgtc acggacagcc tggtacatct ttttgccaac cgttgggcac
    28021 cgctggttgt cgggcgcaac atcggactga tgacgatgga attattcacc ccggcacgcg
    28081 atgtgctggc gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt taaggaataa
    28141 acaatgcaaa gtgttgatgt agccattgtt ggcggcggca tggtggggct ggcggttgcc
    28201 tgcggcttac aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt accggaacct
    28261 ctggcggtag atgcactacc acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa
    28321 ttactcaccc gtcttggcgt ctggcaggac attctcgctc gtagggccag ttgttatcac
    28381 ggcatggaag tgtgggacaa agacagtttt ggtcacatct cgtttgacga tcaaagcatg
    28441 ggctatagcc atcttgggca catcgttgaa aactcagtga ttcactacgc gctgtggaac
    28501 aaagcgcagc agtcgtcaga tattactctg ttggccccca cagaattaca gcaggtcgcc
    28561 tggggagaaa atgaaacctt cctgacgctg aaagatggca gcatgttaac ggcgcgtctg
    28621 gtgattggcg cggacggtgc taattcctgg ttgcgcaaca aagcagatat tccgctgact
    28681 ttctgggatt atcagcatca cgcgctggta gcgaccattc gcacggaaga accgcatgat
    28741 gcggtggcgc ggcaggtttt ccatggcgaa ggcattctgg cctttttacc gcttagcgat
    28801 ccgcatcttt gctcgattgt ctggtcactg tcgccagagg aagcgcagcg gatgcagcag
    28861 gcaagtgaag acgaatttaa tcgcgcgtta aatatcgctt ttgataatcg ccttggctta
    28921 tgcaaggttg agagcgcgcg tcaggtgttc ccgctgacgg ggcgttatgc gcgccagttt
    28981 gccgcacacc gtctggcgct ggtgggcgac gccgcgcata ccattcaccc actggcgggg
    29041 cagggcgtta atctcggatt tatggacgcc gcagagctga ttgctgaact gaaacggttg
    29101 catcgtcagg gtaaagacat cgggcagtac atttacctgc gccgctatga acgtagccgc
    29161 aagcacagcg cggcgctgat gctggctggt atgcagggtt ttcgcgatct cttttccggc
    29221 gctaatccgg cgaaaaaact gctgcgtgat attggtttga aactggccga cacgcttcct
    29281 ggcgttaagc cgcaacttat ccgccaggca atgggattaa acgatttgcc tgaatggctg
    29341 cgttaaaaat ttctcctctg ttgtttattt gatacccatc acactttcat ctcccggttt
    29401 tttcgccggg agattttcct catttgaaat aaactaattt cacctccgtt ttcgcattat
    29461 attttctaat accatgattt tttgatttag tgttttttga cattttttta gctcttaata
    29521 ttgtcttatt caaattgact ttctcatcac atcatctttg tatagaaatt ggtgtatttt
    29581 ttggtttttt attctgtcgc gatttttgca ttttttaacc ataagctaat gtgatgatca
    29641 attttacctt atggttaaca gtctgtttcg gtggtaagtt caggcaaaag agaacgattg
    29701 cgttggggac cgggagtggc tccgatgctg ggttttgtgg tgataatttc accatgaaaa
    29761 agttgtcagc cccgcttatt caatgaggac aagatggcac aacagactcc tttgtacgaa
    29821 caacacacgc tttgcggcgc tcgcatggtg gatttccacg gctggatgat gccgctgcat
    29881 tacggttcgc aaatcgacga acatcatgcg gtacgtaccg atgccggaat gtttgatgtg
    29941 tcgcatatga ccatcgtcga tctccgcggc agccgcaccc gggagtttct gcgttatctg
    30001 ctggcgaacg atgtggcgaa gctcaccaaa agaggcaaag ccctttactc ggggatgttg
    30061 aatgcctctg gcggtgtgat agatgatctc atcgtctact actttactga agatttcttc
    30121 cgcctcgttg ttaactccgc cacccgcgaa aaagacctct cctggattac ccaacacgct
    30181 gaacctttcg gcatcgaaat taccgttcgt gatgaccttt ccatgattgc cgtgcaaggg
    30241 ccgaatgctc aggcaaaagc tgccacactg tttaatgatg ctcagcgtca ggcggtggaa
    30301 gggatgaaac cgttctttgg cgtgcaggtg ggcgatctgt ttattgccac cactggttat
    30361 accggtgaag cgggctatga aattgcgctg cccaatgaaa aagcggccga tttctggcgt
    30421 gcgctggtgg aagcgggcgt taagccctgc ggtctggggg cgcgtgacac gctgcgtctg
    30481 gaagcgggca tgaatcttta tggtcaggag atggacgaaa ctatttctcc tttagccgcc
    30541 aacatgggct ggactatcgc ctgggaaccg acagatcgtg actttatcgg tcgtgaagcc
    30601 ctggaagtgc agcgtgagca tggtacagaa aaactggttg gtctggtgat gaccgaaaaa
    30661 ggcgtgctgc gtaatgaact gccggtacgc tttaccgatg cgcagggcaa ccagcatgaa
    30721 ggcattatca ccagcggtac tttctccccg acgctgggtt acagcattgc gctggcgcgc
    30781 gtgccggaag gtattggcga aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt
    30841 aaagtgacaa aacctgtttt tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg
    30901 gagattgatt gatgagcaac gtaccagcag aactgaaata cagcaaagaa cacgaatggc
    30961 tgcgtaaaga agccgacggc acttacaccg ttggtattac cgaacatgct caggagctgt
    31021 taggcgatat ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc gcgggcgatg
    31081 actgcgcggt tgccgaatcg gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg
    31141 aaatcgtggc ggtaaacgac gcactgagcg attccccgga actggtgaac agcgaaccgt
    31201 atgcaggcgg ctggatcttt aaaatcaaag ccagcgatga aagcgaactg gaatcactgc
    31261 tggatgcgac cgcatacgaa gcattgttag aagatgagta acggctttat tcctcttctg
    31321 cgggagagga tcagggtgag gaaaatttat gcctcaccct cactctcttc gtaaggagag
    31381 aggttcacaa ttcactgcac gtttcaggaa ccatcgctca tgacacagac gttaagccag
    31441 cttgaaaaca gcggcgcttt tattgaacgc catatcggac cggacgccgc gcaacagcaa
    31501 gaaatgctga atgccgttgg tgcacaatcg ttaaacgcgc tgaccggcca gattgtgccg
    31561 aaagatattc agcttgcgac accaccgcag gttggcgcac cggcgaccga atacgccgca
    31621 ctggcagaac tcaaggctat tgccagtcgc aataaacgct tcacgtctta catcggcatg
    31681 ggttacaccg ccgtgcagct accgccggtt atcctgcgta acatgctgga aaatccgggc
    31741 tggtataccg cgtacactcc gtatcagcct gaagtctccc agggccgcct tgaagcactg
    31801 ctcaacttcc agcaggtaac gctggatttg actggac
//
LOCUS       DAJTUN010000036        31180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTUN010000036 DAJTUN010000000
VERSION     DAJTUN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.036"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1027)
                     /gene="yeaG"
                     /locus_tag="OO470_001571"
     CDS             complement(<1..1027)
                     /gene="yeaG"
                     /locus_tag="OO470_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6250858.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIY"
     gene            1463..2209
                     /gene="mipA"
                     /locus_tag="OO470_001572"
     CDS             1463..2209
                     /gene="mipA"
                     /locus_tag="OO470_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6250859.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2299..3060
                     /locus_tag="OO470_001573"
     CDS             2299..3060
                     /locus_tag="OO470_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6250860.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3111..3995)
                     /locus_tag="OO470_001574"
     CDS             complement(3111..3995)
                     /locus_tag="OO470_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6250861.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4079..5074)
                     /gene="gapA"
                     /locus_tag="OO470_001575"
     CDS             complement(4079..5074)
                     /gene="gapA"
                     /locus_tag="OO470_001575"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6250862.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5416..5829
                     /gene="msrB"
                     /locus_tag="OO470_001576"
     CDS             5416..5829
                     /gene="msrB"
                     /locus_tag="OO470_001576"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6250863.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5871..6143
                     /locus_tag="OO470_001577"
     CDS             5871..6143
                     /locus_tag="OO470_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6250864.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6513..7589
                     /locus_tag="OO470_001578"
     CDS             6513..7589
                     /locus_tag="OO470_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6250865.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7616..8995
                     /locus_tag="OO470_001579"
     CDS             7616..8995
                     /locus_tag="OO470_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250866.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9012..10057
                     /locus_tag="OO470_001580"
                     /pseudo
     CDS             9012..10057
                     /locus_tag="OO470_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10078..10914
                     /locus_tag="OO470_001581"
     CDS             10078..10914
                     /locus_tag="OO470_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6250867.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            10919..11866
                     /locus_tag="OO470_001582"
     CDS             10919..11866
                     /locus_tag="OO470_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6250868.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            11876..12856
                     /gene="ydjG"
                     /locus_tag="OO470_001583"
     CDS             11876..12856
                     /gene="ydjG"
                     /locus_tag="OO470_001583"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6250869.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            12993..13751
                     /locus_tag="OO470_001584"
     CDS             12993..13751
                     /locus_tag="OO470_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6250870.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            13869..15227
                     /locus_tag="OO470_001585"
     CDS             13869..15227
                     /locus_tag="OO470_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250871.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15320..15961)
                     /gene="pncA"
                     /locus_tag="OO470_001586"
     CDS             complement(15320..15961)
                     /gene="pncA"
                     /locus_tag="OO470_001586"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6250872.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(15972..16988)
                     /gene="ansA"
                     /locus_tag="OO470_001587"
     CDS             complement(15972..16988)
                     /gene="ansA"
                     /locus_tag="OO470_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6250873.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17155..19011)
                     /gene="sppA"
                     /locus_tag="OO470_001588"
     CDS             complement(17155..19011)
                     /gene="sppA"
                     /locus_tag="OO470_001588"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6250874.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19172..19723
                     /locus_tag="OO470_001589"
     CDS             19172..19723
                     /locus_tag="OO470_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6250875.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19840..20883
                     /gene="selD"
                     /locus_tag="OO470_001590"
     CDS             19840..20883
                     /gene="selD"
                     /locus_tag="OO470_001590"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6250876.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20888..22849
                     /gene="topB"
                     /locus_tag="OO470_001591"
     CDS             20888..22849
                     /gene="topB"
                     /locus_tag="OO470_001591"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6250877.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            22976..24016
                     /locus_tag="OO470_001592"
     CDS             22976..24016
                     /locus_tag="OO470_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6250878.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24133..25476)
                     /gene="gdhA"
                     /locus_tag="OO470_001593"
     CDS             complement(24133..25476)
                     /gene="gdhA"
                     /locus_tag="OO470_001593"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6250879.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            25712..25984
                     /locus_tag="OO470_001594"
     CDS             25712..25984
                     /locus_tag="OO470_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6250880.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(25950..26357)
                     /gene="nudG"
                     /locus_tag="OO470_001595"
     CDS             complement(25950..26357)
                     /gene="nudG"
                     /locus_tag="OO470_001595"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6250881.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26444..27064
                     /locus_tag="OO470_001596"
     CDS             26444..27064
                     /locus_tag="OO470_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6250882.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27073..28380)
                     /gene="ynjE"
                     /locus_tag="OO470_001597"
                     /pseudo
     CDS             complement(27073..28380)
                     /gene="ynjE"
                     /locus_tag="OO470_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28447..29100)
                     /locus_tag="OO470_001598"
     CDS             complement(28447..29100)
                     /locus_tag="OO470_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6250883.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29100..30617)
                     /locus_tag="OO470_001599"
                     /pseudo
     CDS             complement(29100..30617)
                     /locus_tag="OO470_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30590..31180)
                     /locus_tag="OO470_001600"
                     /pseudo
     CDS             complement(30590..31180)
                     /locus_tag="OO470_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7830 a         7457 c         8073 g         7820 t
ORIGIN      
        1 cgtagatttt gatctcttcg gaaatgcgca agcaatacgg caccttcacg atgtaaacac
       61 ggtcgaggaa ggcttcgttg tttttgttat tacggaaagt gacccattcg gacttgttcg
      121 agtgggcgag aataatcccg ttgaacggca gggcggagat accttccgtc ccgttgtagt
      181 taccttcctg agtggcggtt aacaagggat gcagcacttt tatcggtgct ttaaacatct
      241 caacgaattc catgatcccc tgattggcgc ggcacagcgc accggaatag ccgtaggcgt
      301 ccgggtcatt ctgcgcgtag tgttcgagtt tacgaatatc gactttccca accagcgcgg
      361 agatgtcctg gttgttctca tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa
      421 ttgacggcca gaccttcact acccggaact tagtgatatc gccaccaaat tcatgcaggc
      481 gttttgccgc ccatggcgac atgatggtgc cgagataacg gcgaggaatg ccatactctt
      541 tttccagaat ctgagcatct tcctgcggat tgaaaagaca gaacggatga tcgttgaccg
      601 ggctacgctc accgttggcg ctcaatacat aaatcggtac gagctgcatt aatgatttca
      661 gtcgctcagc aagcgatgat ttacccccac ccacaggccc cagcagatac aggatttgtt
      721 tcttctcttc cagcccctga gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt
      781 cttccatgcc gtaaaactct tcaaatgccg gataacgtgc aatgacccgg ttagaaaaga
      841 gtcgagaaag tctggggtcc tgggctgtat cgaccatgac aggctcaccg atagccatca
      901 atagccgctc agccgcgttg gcataagcac tgcgatcttg ccgacaagtg gtaagaaact
      961 cctgcagtgt gaactcttcg tccttggcag cttcatatcg ctggcgatag tgatcaaata
     1021 tattcatggt atgccgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata
     1081 gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc
     1141 atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt
     1201 agatggcaaa tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac
     1261 tcatccataa attttgcata attaatgtaa agaccaggct cgccagtaac gctaaatcca
     1321 tttggctgta agcgcggtgt catccgcggc aggaaaatta aacagttact ttaaaaaatg
     1381 aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca
     1441 tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat
     1501 cgcaacgtct gcaggcgtag cgcacgctga aggtaaattt tccctgggcg caggcgtagg
     1561 tgtcgttgag cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa
     1621 ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga
     1681 cgcaacggat aaactttcaa ttaccgctta ctggtcgccg ctttacttca aagcgaaaga
     1741 cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct
     1801 gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg gcgataccct
     1861 ggataacagc aacggcattg tctgggatat ggcctggttg tatcgttaca ccaacggtgg
     1921 cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga acgaatacta
     1981 ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtggctata acccgaatga
     2041 cagctggagc ccttaccttg agctgagcgc cagctacaac ttcctcggcg actggagtgt
     2101 ttacggtacc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc cgatggtgga
     2161 taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt
     2221 tccagggcat tttgtgcata aaaacagggc gtcacgccct gttttgcatt acggtggtgc
     2281 aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg
     2341 ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa
     2401 gttgctgcac tacgcgcagg cattgaactc ggtttaaccc tcattgatac cgccgaaatg
     2461 tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgagagaag
     2521 gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca
     2581 tgcgaagcca gtttacgccg tctcaatact gactatctcg atctctactt attacactgg
     2641 tctggcagtt tcgcttttga agagactgtt gccgcgatgg aaaaattgat cgcccaggga
     2701 aaaatccgtc gctggggcgt ttctaacctt ggttatgccg atatgcagga actctggcag
     2761 ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg
     2821 attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt
     2881 ccattagccc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa
     2941 attgcacatg tccaacaaca tgcagctgcg cttgaggtcg aactttcgtc agcggaatta
     3001 gctatgctgg ataaggcata tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga
     3061 tgttgatacg tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg
     3121 gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt tggtgacttt ttgcgtttct
     3181 gaagcgtaag ccgtttctac acaaacaaag gtcttgtagc catcgtccgg catgtcgccc
     3241 atgctaactg aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg
     3301 cctacggcga taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc
     3361 agatacacgc gatcggtacg gtcagggaag gtctgaatac cgtcggtcag tacatcttct
     3421 ttcgcgtcat tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg
     3481 atatcaccca cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat
     3541 tcaagatcga tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc
     3601 ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca
     3661 gcatcttcat gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc
     3721 agaccttgtt gtgccgccgg accaaaccac ggccagcaaa ccggtacgcc accgcgaata
     3781 gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca
     3841 ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga
     3901 tcgaccacaa tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt
     3961 tcgatgaccg gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta
     4021 tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt
     4081 atttggagat gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt
     4141 accaggatac cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca
     4201 cggaagtgca aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca
     4261 gaacgccttt catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag
     4321 ttgcagcttt ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc
     4381 ggaacgccat accagtcagt ttgccattca gttctggcag tactttacct acagctttag
     4441 cagcaccggt agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt
     4501 tgtgagacgg gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca
     4561 gaccttcgat gatgccgaag ttatcgttga taactttagc cagcggagct aggcagttgg
     4621 tggtgcagga agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt
     4681 taacgaacat cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag
     4741 cggtgatgtg tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa
     4801 cgtcaacacc cacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga
     4861 tttttttacc gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc
     4921 cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga
     4981 ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac
     5041 ggccaaaacc gttgatacct actttgatag tcatatattc caccagctat ttgttagtga
     5101 ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa
     5161 tcattgcgac aaatcaatcc tgtgcctaag cattacgcga ctgactcgcc tcacttttcc
     5221 tttgggcttg agaccacatg gggtcggcgc cccgaatttt aaagggcaat tacgataaaa
     5281 atgtgatttt catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa
     5341 ttttgccagt tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa
     5401 gtgagatgtg agtaaatggc gaataaacct tcggcagaag aactgaaaaa aaatttgtcc
     5461 gagatgcagt tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta
     5521 ctgcataaca agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat
     5581 tccgaaacca agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa
     5641 gaatccattc gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc
     5701 ggtaactgtg atgcccatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa
     5761 cgttattgtg ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc
     5821 aacggttgaa aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg
     5881 atgacattat caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac
     5941 tggggaaatg gcctgatggc gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac
     6001 tggtgatgct gtggcaagcc cgccataata ccgaagcaca gcatatgaca attgacacaa
     6061 atggtcagat ggtcatgaag agcaaacagc agttaaaaga agattttggt atctcagcaa
     6121 agcctattgc catgtttaag taagtggttc atcccctttt tgccgcggct atgaagggga
     6181 tagccaacaa tgaccccacg actcgtctca attatctcga cccgacctgg ctaagtccca
     6241 gtccgatctc ttgcagtcgc gttcacgcct gaatgacttc tcctgaactt aagtctcaga
     6301 cctatttggc cggtaatccc tctcgaattt gctctttttg cgtcccgcct tccaccaacc
     6361 tgtaacagac ccagccattt tctgcgtaat tgtgatctga gttgtaaaat gcaggtcatt
     6421 atctgtgttg tgcagattta atattgacct caaaaatcat gtgggtaagt atgatggcca
     6481 tctaagcaaa acaaaacaca gaggatgacg caatgaaagc actggctcgg tttggcaagg
     6541 cctttggcgg ctacaagatg attcatgtcc cacaacccat ttgtggcccg gaagacgtag
     6601 tgattgaaat taaagccgcg gcaatctgcg gcgcagacat gaagcactac aatgtcgata
     6661 gcggttctga tgagtttaac tctatccgcg gccatgagtt cgcaggttgt attgcgcagg
     6721 ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc
     6781 acgtttgcgg cgtttgtccg gcctgtgaac agggtgattt tctgtgttgt acagaaaaag
     6841 taaaccttgg tctggataac aatacctggg gcggtggttt ttccaaatat tgtctggttc
     6901 ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga aatccctgat ggtgttgatt
     6961 atgaggacgc agccgtactt gaccctatct gtaatgccta taaatccatc gcgcagcaat
     7021 cgaaattcct tcctggtcag gatgttgtcg tcattggcac tggcccactc gggctgtttt
     7081 ccgtacaaat ggcgcggatt atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag
     7141 atgtggcggt ccgcttcccg gttgcaaaag aactgggggc gacggcagta gtaaatggtt
     7201 ctaccgaaga tatggtggcg cgctgccagc aaatttgtgg caaagataat ctgggactgg
     7261 tgattgaatg ctccggtgcc aatatcgcac tgaaacaagc catcgaaatg cttcgcccga
     7321 atggggaagt ggtacgcgtt ggaatgggct tcaaacctct tgatttctcg attaatgaca
     7381 ttaccgcctg gaataaaagc atcattgggc atatggccta tgactccacc tcatggcgta
     7441 acgctatccg gctattagcc agcggcgcta tcaaagtcaa accgatgatc acgcatcgta
     7501 tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt cgataaaacc gcaatcaaag
     7561 tgatcatgac ttacgacttt gatgaataac tactctcctg aaggaacgtt atcgcatgga
     7621 acagataaca aaaccgcatt gtggtgcacg gctggatcgc ttaccggatt gccgctggca
     7681 ttcgtcaatg tttgccatcg tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg
     7741 tggcttgatc ctcgcgcagc tgaaagcgtt gggctggaca gataattcca ctacagccac
     7801 attctcagca atcacgaccg ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg
     7861 cgacaaaacc ggtcgcagaa atgcgttcat cctctatgag gccattcata ttgcctcgat
     7921 ggttgtcggt gctttctcac cgaatatgga ttttcttatt gcctgccgtt ttgtgatgga
     7981 cgttgggctg ggagctttac tggttacact gtttgctggt ttcaccgaat atatgcccgg
     8041 tagaaatcgc ggaacgtggt ccagtcgggt ttcctttatt ggcaactggt catatccgct
     8101 ctgctcactg attgcgatgg gactcacgcc gctgattagt gcagagtgga actggcgggt
     8161 acaactgctt attcctgcaa tattgtcgct tatcgctacg gcgctggcct ggcgctactt
     8221 tcctgaatcc ccgcgctggc tggaatcgcg cggacggtat caggaagccg agaaagtcat
     8281 gcggagtata gaagaaggcg tcatacgcca gacgggtaaa cctttgccgc ctgtggttat
     8341 tgctgatgac ggtaaagcgc cacaagcggt gccgtattca gccttactga caggagtatt
     8401 actgaaacgc gtgatattag gctcttgtgt gctgattacc atgaacgttg tgcagtacac
     8461 actaattaac tggttgccaa caatattcat gacccagggg attaatttaa aagactcgat
     8521 tgttttaaat accatgagta tgtttggcgc gccatttggt atttttattg ccatgctggt
     8581 gatggataaa attccgcgta aaacaatggg tgtggggcta ttaatcctga ttgcggtgct
     8641 cggatatatc tattcactgc aaaccagtat gttgctcata acgctgattg gtttcttcct
     8701 gattactttc gtctatatgt acgtttgcta tgcctcggca gtgtatgtcc ctgaaatctg
     8761 gccgacagag gccaaactcc gtggctccgg tctggcgaat gcggtagggc gaatcagtgg
     8821 tattgccgca ccttatgccg ttgcagtgct gctcagtagt tatggtgtaa cgggagtctt
     8881 tattcttctg ggggctgttt caaatattgt cgcaattgct atcgccacca ttggaattga
     8941 aaccaaaggt gtctccgttg aaagtttaag tattgatgca gtagccaata aataatacgg
     9001 gtggattcaa aatgaaaaat tcaaaagcaa tattgcaggt gccgggcaca atgaaaatta
     9061 tttcagcaga aataccagtg cctaaagaag atgaagtttt gattaaagta gaatatgtcg
     9121 gtatttgtgg ttcagatgta catggttttg aatcaggccc gtttattccg cctaaagacc
     9181 caaatcaaga aattggcctg ggtcatgaat gcgccgggac ggttgtggct gtgggaagcc
     9241 gcgtgcgcaa atttaaaccg ggggatcggg taaatatcga acctggcgtt ccttgcggtc
     9301 actgtcgtta ctgtctggaa ggcaaatata acatctgccc ggacgttgat tttatggcga
     9361 cacaacccaa ctaccgcggc gcattaacgc actatctgtg tcatccggag agctttactt
     9421 acaaactgcc cgacaatatg gacacgatgg aaggggcgct ggtggagcct gccgcagtcg
     9481 ggatgcatgc cgcgatgctg gcagatgtta aaccgggtaa gaagataatt attctgggag
     9541 caggttgtat tggtttgatg acgttgcaag cgtgcaaatg cctgggcgca acggaaattg
     9601 ccgtcgttga tgtactggaa aaacgtctgg caatggggga acagcctggt gcgacagtgg
     9661 ttattaatgg cgcaaaagag gacactattg cacgctgtca gcaatttacc gaagacatgg
     9721 gcgcagatat tgttttcgaa acagcgggtt ctgcggtcac cgttaaacag gcacctttaa
     9781 tctggtaatg cgcggcggta aaattatgat tgttggtact gtacccggcg attcggcaat
     9841 caatttcctc aaaatcaatc gcgaagtcac tatccagacg gtattccgct atgccaatcg
     9901 ttatccggtc acgattgaag ctatttcttc agggcgattc gatgtgaaat cgatggtgac
     9961 gcatatttac gattatcggg atgtacaaca ggcatttgaa gagtcagtta acaacaaacg
    10021 cgacattatt aaaggcgtta ttaaaattag cgattaatta atacgggaga acagaacatg
    10081 ctcgcagata tcagatattg ggaaaacgat gccacaaata aatattatgc aattgcccat
    10141 tttaacgtat ggaatgcaga aatgttgatg ggcgttaaag acgcagccga agaagcgaaa
    10201 tccccggtta ttatttcttt tagtacaggt tttgtcggta acacctcatt tgaagatttc
    10261 tctcacatga tggtatcaat ggcacaaaaa gcaacggtgc cggtaataac tcattgggat
    10321 catggtcgga gtattgagat tattcataac gcctggactc atggtatgaa ttcattaatg
    10381 cgtgatgctt ctgcatttga tttcgaagaa aatattcgtt taaccaaaga ggctgttgat
    10441 ttctttcatc cgctgggtat tccggtagag gcggaattag ggcatgtcgg taatgaaacc
    10501 gtttatgagg aagcgctggc gggctatcac tataccgatc ctgaccaggc ggctgaattt
    10561 gttgaacgta cgggctgtga ctcattggct gtcgccatcg gcaaccagca tggggtttat
    10621 acgtcagagc cgcaattgaa ctttgaggtc gtcaaacgcg tacgcgatgc tgtttctgtt
    10681 ccgctggttt tgcatggcgc atcggggatc agtgatgccg acattaaaac tgcaatttcg
    10741 ttgggtattg cgaaaatcaa cattcatacg gagctctgtc aggccgcaat ggtagcagtg
    10801 aaagaaaacc aggatcagcc tttcctgcat ttagaacgtg aagtacgtaa agctgtaaaa
    10861 gaacgggcat tggaaaaaat tgaactgttc ggttctgatg gcaaagcgga ataatgatat
    10921 ggataatctc gacgttattt gtataggtgc cgctattgtt gatattccat tacaaccggt
    10981 cagtaaaaat atctttgatg tagattctta tcctcttgaa agaatcgcaa tgagcaccgg
    11041 gggcgatgca ataaatgaag caacaattat ttctcgtctg gggcatcgta ccgcattaat
    11101 gagccgcatt ggtaaagatg cctcagggca atttattctc gaccattgtc gcaaagagaa
    11161 tattgatatt caaagcctga aacaggacgt cagcatagat acatctatta acgttggact
    11221 ggtgacggag gatggcgagc ggacgtttgt cacgaaccgt aacggcagtt tgtggaaatt
    11281 aaatattgac gatgttgatt ttgcgcggtt tcctcaggcg aaattattat cactggccag
    11341 tattttcaac agtcctctat tggatggtaa agcgctaaca gaaattttta cgcaagcgaa
    11401 agcccggcag atgatcattt gtgccgatat gatcaaaccg cggttgaatg aaacgctgga
    11461 tgatatttgc gaagcattga gttatgtcga ttatctgttt ccaaattttg ccgaggaaaa
    11521 attactcaca gggaaagaga cactggatga aattgctgac tgctttcttg cgtgcggcgt
    11581 aaaaacggtg gtgattaaaa cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac
    11641 gatgaaggtg ccggcggtcg caggaataac cgccatcgac accattggcg cgggcgataa
    11701 ctttgcttca ggttttattg cggcactgtt agaaggcaaa aatctgcgtg aatgcgcacg
    11761 ctttgccaat gcaacggcgg ctatctcggt tctaagcgtc ggtgccacca ccggcgtaaa
    11821 aaacagaaag ctggtcgaac aattgctgga agaatacgaa ggataatgaa ggcaaatgaa
    11881 aaagatacct ttaggcacga cggatataac gctttcgcga atggggttgg ggacatgggc
    11941 cattggcggc ggtcctgcat ggaatggcga tctcgatcgg caaatatgta ttgatacgat
    12001 tcttgaagcc catcgttgtg gcattaatct gattgatact gcgctaggat ataactttgg
    12061 caatagtgaa gttatcgtcg gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt
    12121 agaaaccaaa tgcggcattg tctgggaacg aaaaggaagt ttattcaaca aagttggcga
    12181 tcggcagttg tataaaaacc tttccccgga atctatccgc gaagaggtag aagccagctt
    12241 gcaacgtctg ggtattgatt acatcgatat ctatatgacg cactggcagt cggtgccgcc
    12301 attttttacg ccgatagctg aaactgtcgc agtgcttaat gagttaaagg ctgaagggaa
    12361 aattcgcgct ataggcgctg ctaacgtcga tgctgaccat atccgcgagt atctgcaata
    12421 tggtgaactg gatattattc aggcgaaata cagtattctc gaccgggcaa tggaaaacga
    12481 actgctgccg ctatgtcgtg ataatggcat tgtggttcag gtttattccc cgctagagca
    12541 gggattgttg accggcacca tcactcgtga ttacgttccg ggcggcgctc gggcaaataa
    12601 agtctggttc cagcgtgaaa acatgctgaa agtgattgat atgcttgaac agtggcagcc
    12661 actttgtgct cgttatcagt gcacaattcc cactctggca ctggcgtgga tattaaaaca
    12721 gagtgattta atctccattc ttagtggggc tactgcaccg gaacaggtac gcgaaaatgt
    12781 cgcggcactg aatatcaact tatcggatgc agacgcaaca ttgatgaggg aaatggcaga
    12841 ggccctggag cgttaaatac tcaacacgat tcacctctgt ttctgtgaaa actgtgatga
    12901 aaatctgtga ttcaaatagg ttattttgaa agtaaacatc ggttatgcga taatcgcgct
    12961 aatgtgatgt aaaaagatac aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa
    13021 ttaagcaaat ggttgccaac gataaaaagg tgacagtctc aaatttgagt gggatttttc
    13081 aggtaaccga agaaaccatt cgccgcgatc ttgagaagct ggaagatgaa ggctttttga
    13141 ccagaaccta tggtggtgct gttttaaata cagcggtgtt gacggagaat atccattttt
    13201 ataagcgcgc ttcatcgttt tatgaagaga agctgcttat tgcacgcaag gcactaccct
    13261 ttatcgacaa taaaaccacc atggcagccg attccagtag taccgtaatg gaattgctca
    13321 aattgttaca ggaccgtagt gacctgacgt tgctaaccaa ctcggcggaa gcgattcatg
    13381 ttctggctca gtcagaaatt aaagtcgttt caacgggtgg ggaactaaac aaaaatacac
    13441 tttccctgca aggaagaatt actaaagaga tcatcagccg ctaccatgtc gatatcatgg
    13501 taatgagttg taaaggtctt gatattaaca gcggcgcgct ggactctaat gaagcggaag
    13561 ctgaaatcaa aaagacaatg atccgtcagg cgacagaagt tgcgttattg gttgatcact
    13621 ctaagtttga tcgtaaagct tttgtccagt tagctgactt tagtcatatt aattatataa
    13681 taactgataa atcaccgggt gcagagtgga tagcattttg caaagacaat aatatccagc
    13741 tggtgtggta atttaagcac acgttgtcaa tattatttgt tcctgcgact ggaaataaaa
    13801 tgatgataaa gatgcggcaa gtgaatttgc cgcaattgct accttttata gcaaatccga
    13861 ggcacccaat ggaacaatat gatcaaattg gcgcaagact ggaccgcttg cctttggccc
    13921 ggtttcatta tcgtatattt ggtataataa gctttagtct gttattaacg gggtttttga
    13981 gttactcagg taatgtcgtc ttagcaaagc tggtaagcaa tggatggtca aataatttcc
    14041 tctatgccgc ctttacctcg gcattaatgt ttggttattt catcggctca cttactggtg
    14101 ggtttattgg tgactacttt gggcggcgca gggcgtttcg cataaatctt ctcatcgtcg
    14161 gtattgctgc aacaggggcc gcttttgtcc ctgatatgta ctggctcatt ttctttcgct
    14221 tcctgatggg aacaggaatg ggggcgctga ttatggttgg ctatgcctca tttacggagt
    14281 ttatccccgc gacggtgcgt ggaaaatggt ccgcgcggct ctcatttgtt ggtaactggt
    14341 cgcccatgct gtctgcggcg ataggcgtgg tggttatcgc tttttttagt tggcgaataa
    14401 tgtttcttct gggtggtatt ggcatactgt tagcctggtt tctctcaggt aaatacttta
    14461 tcgagtcgcc acgatggctg gaagggaaag ggcaaatcgc cggtgcagaa agccaacttc
    14521 gtgaagtaga gcagcaaatt gaaagggaga agagtattcg tttaccccag cttactttga
    14581 accagagcaa cagcaaggtt aaggtaatca agggtacctt caggctcctg tttaaagggg
    14641 aaatgttacg acgtacatta gttgcgataa ctgttttaat tgcaatgaac atttcgcttt
    14701 ataccattac cgtatggata ccgaccatat ttgttaactc cggcattgat gtcgataaat
    14761 caatattaat gaccgctgtt attatgattg gtgctccggt aggaatattt attgcagcat
    14821 taattattga tcattttcct cgtcgattat ttggctccgc cttacttatt attattgccg
    14881 tgttaggcta tatctattca agtcagacta cagagtgggc gattttaatc tatggtctgg
    14941 tgatgatctt ctttttatac atgtatgttt gcttcgcgtc ggcggtttat atcccggagc
    15001 tttggccaac gcatttacgc ctgcgcggtt cgggtttcgt taatgccgtc ggacggatcg
    15061 tcgcagtttt cacgccctat ggcgttgcgg cattattaac acattatggg tcgatcacgg
    15121 tgtttatggt gcttggtgtc atgttagtgc tctgtgcgct ggttctctcc atttttggca
    15181 tcgaaacgcg gaaggtgtcg ctggaagaga tttctgaggt gaattaaatc tttcgttgat
    15241 ggcatcaatt gccagatgta ccactgacgt gttttatttg gcctacaatg ccagtacaga
    15301 attgtaggcc agcgtaaaat tacccctgcg tctcttccca gtctgccagc gtatatagcg
    15361 ttgccccagc tgctgacatc tccataaacg cgtgtgcact gtcctggggc tggatattca
    15421 cgccacgaca gccatcggta atcacgttta ccttataacc taactgtaac gcgtccagca
    15481 cggtaaactt cacgcaatag tcagtagcca ggcccataac gatcaattca tcgatttcat
    15541 gatcgcgcaa ccagtcatca agcgcggttt tctgccgacg tccgttatca aaaaaggcac
    15601 tgtaactgtc aactaaagga ttttcgcctt tatggaacac cgctgcgatc tctttttgat
    15661 tcagtaacgg atgtaattgt gcgccttcac tgttctgcac acagtgttca ggccagaaag
    15721 tttgccgcag gccgccgagt tggcctggcg tataaggctc tacgccgtgc tggctggcaa
    15781 agctgccgtg attcgccgga tgccagtcct ggctggcgat aaccgcttct ccgcgcgact
    15841 ggcaccagtc aataaggcgg ttagcaacct ccaccgtact gtcaccttcc ggtacggcga
    15901 gcgcgccacc agcacagaaa tcattttgta aatcgaccag taacagggcg cgatggggca
    15961 ttgcagtctc cttaatcatc cggcgtcagt tcgccgcgca ggttttggct catggccttg
    16021 cgaatggttt cagtatccag ttcctggctc agcaggtaat gcagtttggt tagcgtggct
    16081 tctacagtca tatctgcacc gtcaataacg ccggcatggg cgagggcgtt accggtggca
    16141 taaccactca tattcacttt accggacata cattgtgtca ggttgaccac cacaataccg
    16201 cgatcgctgg cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc attacccacg
    16261 ccataggagc gcagaatcaa tgctttcacc ggttggcgca gaaaattgcg cacgacgtca
    16321 gcagaaatcc ctggataaat cgtcaccacg ccaattggtt gtggcgtgat tggatgaacg
    16381 attaattccc cttcaccgtg cggggcgggt ggcgtattca aacgacgaat atggatacca
    16441 gcttccagta acggaggaag gtttggagag gcaaacgcat caaaaccatc ggcatgggct
    16501 ttggtagtgc ggttgccgcg atacaatcgg ttattgaaaa agagcgttac ttcgttgatc
    16561 ggataattcg ccgcaacgta caacgcattc agcagattaa tttgtccgtc agagcgtaac
    16621 tcagccagcg ggatttgtga ccctgtcaca atgaccggtt taccgagatt ctcgagcatg
    16681 aacgacagcg cagaggcggt atacgccatc gtgtcggtac cgtgtagaat gacaaaacca
    16741 tcatagtcgt catagtgcgc tttaatatct tcagcaatat gctgccagtc ttccggcgtc
    16801 atatcagaag aatccatcaa cggcgtatat tcatgaatgg tgaaatctgg catctccggg
    16861 cgatggaatt ccggcatcag cgccagttgg cgttgtagat gacctgacac cggaatataa
    16921 ccctgctcgg aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta aattgatttc
    16981 ttttgcatga tattgatatg aatgatatcg aaagagcaaa agtatatacg tgaggcagga
    17041 ggagcgcacc tgatactatg cggataagga aatatggggc gagacagcgg gaagaggatt
    17101 cgtcgaggta aaaaataatg tggccgacga tctgtcggcc actcagtaca agacttaacg
    17161 cacgttggcg caggtcaggc aaaacgcata acggttttgt gggtcgttaa acgcggccag
    17221 cttgtcgctt tcacttttaa cggtagaggc taccgaggca agcggtgcag gtaacatggc
    17281 ctggaacgca tctggcaaca ttgcccggac agaaccagac atgttgtcca tcactttgtc
    17341 gaagaaggtc ggttcatcaa cgtagtattc cagatgccac tgtttcactt ttgcgagctc
    17401 tgctgctttg gcaaccgcat catcgaaatc cccgagacta tcgaccagcc cgttagcttt
    17461 tgcatcctga ccggtccaga cgtggccctg ggcgatttta tcaatttgct ccggcgtcga
    17521 atgacgcgca tcagcaacca gcgtgataaa gcgtttatag ccattctcaa tgcttaattg
    17581 catcatctgc tgcgcttccg gcggcagtgc cctggtgata gaaacatccg ccagcggtga
    17641 agttgagaca ccatcagtat gaacaccaat cgaatccaga ctattttcta cggtggtgat
    17701 cacgccgaag ataccgatag aaccggtcag ggtgctgggg ttagccacaa tgtaattagc
    17761 tggcgtggaa atccagtaac caccagatgc cgccatgccg cccatcgata caaccacaga
    17821 cttacccgct gcccgggctg ctgccagttc agcgcgaatc acttcagacg cggtaacgct
    17881 gccgcctggg ctattaacac gcaggacaat cgctttcact ttcgggtcaa ggcgagcgtc
    17941 gcggatttgt gccgcagtgg tatcaccgcc aacattcccc tgagtttcct cgccatccat
    18001 aattgcgcca ttagcgaaga cgacaccgat gctgtcaccg gtatctgccg gcgttttcaa
    18061 tgcgtaatcg taataactga tggcgcgata atttttatca gtcttactcc agccgaactc
    18121 tttggtcaga gctttttcga tttcggcact cgatgccagt gcatcgacca gcttgttttc
    18181 cagtgcatat ttcgcggtat cgccaccggt tttggttaaa ccctcaagca acccttgcgc
    18241 gccagggaat acctgctgag cagggatctg ccggttagcg gcaacagtat tcagatagtt
    18301 ttgccacagc tcaccaatcc agcggctgtc agcttcgcgg gctgccggtg acatatcatc
    18361 acgaataaac ggttcaacgg cagatttata cgtacccacg cggaacacat gggtggaaac
    18421 tttcagctta tccagcaacg atttgtagta cagaccgttg gtagcaaaac cgtgcagatc
    18481 aaccacgcct tgcggtgaca gccaaatttt attggcgaaa ctggcgagat aatattgccc
    18541 ctggctgtag ttctcgccaa cggcataaac cggtttcccg ctgtcacgaa actctttcag
    18601 ggctttgccg atgtactgca tagacggctg gtcgccgcct gcgaagtttt tcagatccat
    18661 cacgataccg gtgatattgc ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa
    18721 cagtgagttt tcctgcagac gatcggaact ggcaccaagc agctggcggc ttaatttact
    18781 aaaccgctga gaactgtctg gtttatcgac gatcacacca gaaatgtcca gcagcagtgc
    18841 gccacgactg gccgtttctt tcgaatcacc accactgacc tgcatccaga tccccacacc
    18901 aaccagtacg aggaaaataa agaacaggtt aagtaccatt tcacggacga aattcagcag
    18961 acgccacgtc catttaaaaa atccggcaat aaatcgccaa agggttcgca tgtattctcc
    19021 caacttaagg tcacacctgt cacaggcgca atagatgtgg ttatcgtaat gacccaaccg
    19081 gcacttgtca gcaggaattg cggactgtgc tgtaacaaaa tccactctcg tgttaatttt
    19141 gtgaataaat atcacgacag gagttaatca aatggatgca ctcgaactat tgatcaatcg
    19201 ccgtagcgcc tcccgcctgg ctgaacccgc gccaacgggt gaacaactgc aaaacatcct
    19261 gcgtgcgggt atgcgtgcgc cggaccataa gtccatgcaa ccgtggcatt tttttgtgat
    19321 tgaaggggaa gggtgcgagc gtttcagcgc cgtactggaa cagggggcga ttgctgccgg
    19381 tagtgatgat aaagctatcg acaaagcccg taatgcgccg ttccgcgcac cgctcatcat
    19441 cacggtggtg gcgaaatgca aagaaaatca taaagtcccg cgatgggaac aggaaatgtc
    19501 tgccggatgc gcggtcatgg cgatgcaaat ggcagccgtt gcccagggat ttggcggcat
    19561 ctggcgcagt ggcgcattaa ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga
    19621 gcaggataaa attgtcggtt ttctctacct cggcacgccg cagttgaaag catctacgtc
    19681 gattaacgtc ccggacccga cgccgtttgt aacttatttc tgataattgt cgcgaaactg
    19741 tctggattgt gagcaaaggc gcaggaattc agacactctc acttatcact tcacggaatg
    19801 agggttacca tacccggatt gcattgacag gagatgtcca tgagcgagaa ctcgattcgt
    19861 ttgacccaat acagccacgg agctggttgc ggctgtaaaa tttccccaaa agtgttggaa
    19921 accatcctgc acagtgagca ggcgaagttt gttgatctga atttgcttgt gggtaatgaa
    19981 acccgcgacg atgcggcggt gtacgatctg ggcaatggca ccagcgttat cagtaccacc
    20041 gacttcttta tgccgatcgt tgataatcct ttcgattttg gccgcattgc ggcgactaac
    20101 gccatcagcg atatcttcgc gatggggggc aaaccgatta tggcgattgc gatcctcggc
    20161 tggccgatta acaaactttc cccggaaatt gcccgcgaag taaccgaagg tggacgctat
    20221 gcatgccgtc aggcgggtat tgcgctggct ggcggtcact ccatcgatgc gccggagccg
    20281 atttttggtc tggcggtaac ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc
    20341 gcacaagccg gatgcaaact gttcctgacg aaaccgctgg ggatcggcgt tcttaccacg
    20401 gctgagaaaa aatcactgtt gaaaccagaa catcagggac tggcgacgga agtgatgtgc
    20461 cggatgaaca tcgcaggcgc gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac
    20521 gttacgggct ttggtctgct gggccacttg agcgaaatgt gtcagggggc tggtgtgcag
    20581 gcacgcgtcg actatgaagc gatcccgaaa ctccccggtg ttgaagagta cattaagttg
    20641 ggcgcagtac ctggcggcac tgaacgtaac tttgccagct acggtcatct gatgggtgaa
    20701 atgccgcgtg aagtgcgcga tctgctgtgc gatccgcaaa cttctggcgg tttgctgctg
    20761 gcggtcatgc cggaagcaga aaatgaggtt aaaactacag ccgccgagtt tggcattgaa
    20821 ctgacggcga ttggcgaact ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt
    20881 taatttaatg cggttgttta ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt
    20941 cctgcccaaa ccgcaccgga aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt
    21001 gacttggtgt atcggtcacc tgcttgagca ggcgcagcca gacgcctacg acagccgcta
    21061 tgcgcgctgg aatcttgcgg atttgccgat tgtcccggaa aagtggcaat tacagccacg
    21121 accctccgtg accaaacaac ttaacgtcat caaacgcttc ctgcatgaag ccagcgaaat
    21181 cgttcacgcc ggggacccgg atcgtgaagg gcaattgctg gtggatgaag tgctggacta
    21241 tctgcaactg gcaccggaaa agcgccagca ggtgcagcgt tgcttgataa acgacctgaa
    21301 cccgcaggcg gttgagcggg cgatcgaccg tcttcgttcc aacagtgagt ttgtgccatt
    21361 gtgcgtttct gcgctggcgc gagcgcgtgc cgactggctg tacggcatca atatgacccg
    21421 tgcgtatacc attctcggtc gcaatgccgg ttatcagggc gtactttccg tgggacgcgt
    21481 gcagacaccc gtgctcgggc tggtggtgcg ccgcgatgaa gagattgaaa acttcgtggc
    21541 gaaagacttc tttgaagtca aagcacatat cgtgacacct gccgatgagc ggtttaccgc
    21601 tatctggcaa ccgagcgaag cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca
    21661 tcgtccactg gcggagcatg tggttaaccg cattagtggt caaccggcta ttgtcaccag
    21721 ctataacgat aaacgggaat cagaatccgc gccgctgccg ttttcgcttt cggcgttgca
    21781 gattgaagcg gcaaaacgct tcggtctgag cgcgcagaac gtgcttgata tctgccagaa
    21841 gctgtacgaa acgcacaagc taatcactta tccgcgttcc gattgtcgct atttgccaga
    21901 agaacatttt gccggacgcc acgcggtgat gaatgccatc agcgttcatg caccggatct
    21961 gttgccgcag ccagtggtag atccagatat acgcaaccgc tgttgggatg acaaaaaggt
    22021 cgatgcgcac cacgccatca tcccgaccgc acggagttct gcgatcaacc tgacggagaa
    22081 cgaagcgaag gtctataacc tgattgcccg tcaatacctg atgcagttct gcccggatgc
    22141 ggtgttccgc aagtgtgtta tcgaactgga cattgccaaa ggcaaatttg tcgctaaagc
    22201 ccgttttctt gctgaagcag gctggcgcac gctgttaggc agcaaagagc gcgatgaaga
    22261 aaacgacggc acgccattgc ctgtggtggc gaaaggcgat gagttgctgt gtgaaaaagg
    22321 tgaagtggta gagcggcaaa cccagccgcc gcgccatttt accgatgcaa cactgctttc
    22381 ggcgatgacc gggatcgcgc gctttgtgca ggacaaagat ctgaaaaagg tccttcgtgc
    22441 gaccgatggt ctggggacag aagcaacccg tgccgggatt attgaactgc tgttcaagcg
    22501 tggttttctg accaaaaaag ggcgctatat ccactccacc gacgccggaa aagcgttatt
    22561 ccattcgctg ccagaaatgg cgacgcgacc ggacatgacc gcgcactggg aatcggtgct
    22621 gacgcaaatc agcgaaaagc agtgtcgcta tcaggacttt atgcagccgc tggtggggac
    22681 gctatatcag cttattgatc aagccaaacg tacgccggtg cggcagtttc gcggcattgt
    22741 ggctccgggc agtggtggca gtgctgataa gaaaaaggct gcaccgcgta aacgtagtgc
    22801 gaaaaaaagt ccgccagcag atgaagccgg aagcggggcg atagcgtaag cgagcgaatc
    22861 tttcgtgcta ttcgagtcat attctgaaat atccagcgga tcaagaaaat tcgttggata
    22921 tttttttgca tggataaaat tatcgcctct aaagtatgta ataacaggga atgtgatgaa
    22981 aaaagtttta ttgcaaaacc atcctgggag cgagaagtat tcttttaatg gctggggaat
    23041 atttaatagt aattttgaac gggtgattaa agaaaataag gccatgctgc tttgtaagtg
    23101 ggggttttat ttaacatgtg ttgtcgctgt aatgtttgta ttcgcagcga taacatccaa
    23161 cggtttgaat gaaagaggcc tgattaccgc gggatgctct tttctttatc tattaattat
    23221 gatggggctt attgttcggg ccggttttaa agcaaaaaaa gaacaactgc attattatca
    23281 ggctaaaggt attgagccgc tcagtatcga gaagttacag gcgctacaat tgatcgcacc
    23341 ttatcgattc tatcataagc aatggtctga aacgctagag ttctggccgc gaaagcctga
    23401 acctggcaaa gataccttcc aatatcatgt gcttcctttt gactcgatcg atatcatcag
    23461 taaaagacgc gagtctttag agaatcaatg gggtatcgaa gatagcgaaa gttattgtgc
    23521 cttaatggag cattttcttt ctggcgacca tggagccaat acctttaaag caaacatgga
    23581 ggaagcccca gagcaggtta tcgccttgtt gaataaattt gctgtttttc cctcagacta
    23641 tatctctgat tgcgctaata atcgctccgg taaatcctcg gcgaagctaa tatgggcggc
    23701 ggaattatca tggatgatct cgatatcaag cacagctttt caaaacggga caattgaaga
    23761 agaactggcc tggcattata taatgcttgc ttctcgaaag gcgcacgagt tgttcgaaag
    23821 cgaagaagat tatcaaaaaa atagtctaat gggatttctt tactggcata tctgctgcta
    23881 tcgcagaaag ttaacggatg ctgaacttga agcgtgttat cgttacgaca agcagttttg
    23941 ggagcactac agtaagaaat gccgttggcc cataagaaat gttccctggg gagcatcatc
    24001 cgttaaatac tcataaacgc ctgaaatttt gccgggggcg ctgcgcttga ccggcctaca
    24061 ttagagcgta actgcaatga attgtgccca tctgtaggcc tgataagcgt agcgccatca
    24121 ggcatttaca acttaaatca caccctgcgc cagcatcgca tcggcaacct tcacaaaacc
    24181 ggcaatgttc gcgccctgca cgtagttggt ttgctcacct tcaccaccat gctcaacaca
    24241 ggcatggtgg atatccagca tgatgtgatg caaacgtgcg tcaactttct cggctttcca
    24301 gcccaggcgc gcagcgtttt gtgccatttc caggcccgac gttgctacac cgccagcatt
    24361 agccgcttta cccggtgcaa acagtacgcc tgcctgctgg aacagttcag tcgcttcgat
    24421 ggtggtcggc atatttgccc cttcagcgac ggctttaacg ccattggcga taagctgatg
    24481 cgcggcgtca acatccagtt cgttctgggt ggcgcaaggc agggcgatat caaccggtac
    24541 agaccacggc tgttggcctt cgagatagac cagaccaaat tctttggcgt aatctgccac
    24601 tcgaccatcg cggctggctt tgatttcgat aagacgtgcc agtttctctt tcgtgaatcc
    24661 gctttcatca actacagtgc cgctggagtc tgacgcagtg atcacacgag caccaaattc
    24721 catcgctttt tcgatagcgt actgggcgac gttgccggag ccagaaacgg aaacgctcat
    24781 cccttcaaaa cccataccgt ggcgttttaa cattgcttct gtgaaataaa ccagaccgta
    24841 gccggtagct tccgggcgaa taagactacc gccaaatgaa aggcccttac cggtgaagac
    24901 gcaggcggta ttgttggaga gctttttcat catccccgcc ataaagccga cttcacgacc
    24961 accaacgccg atatcacctg ccggaacgtc ggtatccgcg cccaggtggc gatacagttc
    25021 agtcatcagc gtctggcaaa aacgcatcac tttaccttcg ctttttcctt tcggatcgaa
    25081 atcgctgccg cctttaccac cgcccatcgg cagggtagtc agggcatttt tgaaggtttg
    25141 ttcaaagccg aggaatttga gaatggaaag gttaactgac ggatggaagc gcataccgcc
    25201 tttgtacggg ccgatggcag agctgaactg cacacgccat gcacggttga cctgtatctg
    25261 gttgcgatca tcaacccata ccacgcgaaa ctggatcacg cgctccggtt caaccagacg
    25321 ctccagtaat gacatctggc gatattttgg attttgttca agaaaaggcc agagtgtggt
    25381 cattacttca cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct tttggacatg
    25441 gttgaggaat gactccagag aatatgtctg atccatagat ataaaaacct tatatattaa
    25501 tacgattgtg cttatgttgt ttatgtcatg tgcttttgca gttttcgact ataccatcaa
    25561 gaatgtgacg gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa cgagagtaat
    25621 ctcataacca aatgcgataa ctcagacgct aaagttttgc cacccggcta ccgttatcag
    25681 tagcagagac aataacgtta aggagttccc tgtgagtcga gcattgttcg ccgtagtatt
    25741 agcatttcca ttaattgcac tggctaaccc gcattatcgt ccggacgtgg aggtaaacgt
    25801 accgccagaa gtgttcagtt caggcgggca gtcagcgcag ccctgtactc agtgctgcgt
    25861 ttatcaggat caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa tactgctgca
    25921 atgccagcgt gatgacaaaa cccttagcac taatccgctg ctctggcggc gcgtaaagcc
    25981 ataaacgcct ctaataatgg aatgtcagca ggggccagcg gatattgcag cgcctcttca
    26041 ggtgagcacc agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg gaagtcgggt
    26101 acgtgccagg catgaagatg gataatccgg cccgaaactt ctcgctgatg gctggcaaca
    26161 tactcaccca cagttgcttc gatgtccaat tcttcgttta actcacgcac cagcacctgc
    26221 tgctggcttt catccagctc gactttacct ccggcaaact cccataatcc cgcctgatcg
    26281 ctctgggcgg ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat ggcggcaaca
    26341 acttcaatca ttttcataaa ttacacacgg gcagataagc gaaaaatgcc ataatatccg
    26401 cactgttgct caatcagaaa ggctttatat ttaaggagtg cctgtgctag accgccatct
    26461 tcatccccgg attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata aaccgggcat
    26521 tacgccagat ggtttaacat tagtgggatt tgccatcggc gtgctggctc tgccgtttct
    26581 ggcgctgggc tggtatctgg cggcgctagt cgtcattttg ttgaacaggc tgcttgatgg
    26641 tctggacggt gcgctggcgc ggcgcagagg gcttaccgac gcgggcggct ttcttgatat
    26701 ttctctcgat tttctctttt acgcgctggt gccgtttggc tttattcttg ctgcaccgga
    26761 gcaaaatgcg ctggcaggcg gctggttgtt gtttgcgttt atcggcacgg gcagcagttt
    26821 tctcgccttt gctgcactgg cagcaaaaca tcagattgat aaccccggtt atgcacataa
    26881 gtcgttttac tatctggggg gattaaccga aggcacagaa acgatcttac tgtttgtgct
    26941 gggatgttta ttccctgcat ggtttgcgtg gtttgcgtgg atcttcggtg cgttgtgctg
    27001 gatgacgacc tttacgcggg tgtggagtgg gtatctgacg ctgaagtcgc tccagcgtca
    27061 gtaattgcaa tgttatttgc tactgcccgg gccgcgttca ccggttgcaa ccggattttt
    27121 tggatcgctg ctccattcgt accagccgcc gtcatacacg gaaacattct tccaacccat
    27181 ggcgcgtgcg tacataaagg tttcggacgc gcgccagccg gtgccgcagt agaatgaaac
    27241 ttgctgatct ggtttgatat tccatgcttt ccacatagcg gtaatatcat cggcgctacg
    27301 catggtgcca tccgggttat agaaatcttc catatgcgtc gagtcgctac cagcgtgtcc
    27361 ccaacgtgct ccggctattt caccttttgg tttaatatag ctgtaaccgc tggtcgtacc
    27421 gataaattct ggccacgaac gaatgctcac cagcgatgca tcctggcgat gcagcagtcc
    27481 acgggcctgt tccatatcga gcatcaactg cggttgtgcc gggatcttca cgccgaaatc
    27541 cggttccgct ttcactttcg gtggcgttcc tcgctcaaca ggcagtcccg cgtcggacca
    27601 ggtttgccag ccgccatcca gcagacgcac atctttcacg cctgcgtaga gcataatttg
    27661 cgccacacgc gctccagcgt atacgtcacg cccgtacaga atgaccgtgg tgtcatggcg
    27721 aatgccgtgt tttgccagca tcgcttttag ttgttcatca gaaactttgt tccacagcgg
    27781 ttcgctttcc acttcgttgg tatcgatgta gtcagcgcca ggaatatggc tgataaggta
    27841 aagcttagga gcgccctagg ccgcttcaat gactttccag tcaccgacag gtttcgccgt
    27901 aacctcttta ccttgttgca ggtcatgcag ccattgcgga taaaccagct gctcaaagtg
    27961 cggcagtttt tgtagacggg aaggttcgct tagcgcgtca ctcagaatgg agatatgcgt
    28021 aaaacctgct ttttgcagtc gcgttttgac ggcgtcgaca tctttgccat taccgtacag
    28081 cgccaccggg gcatcggctt tcaggttatg ttgcttgatc cacgcgttga gctgttcggt
    28141 gctcattttg tcgagccagc tagcagagag gtttaaggcg gcaggttcat gaccagaagg
    28201 gccatttaag gtttgtggcc agccgttata aaacgcgctg gggcgagtat ctatcgcttt
    28261 gccattttgt tgctgaagtt ggtcaagtgt aagaggcttc gccagttcag cggcccacga
    28321 agaagcgcaa gccagcccta aagccattgc cagcgcggtc atttgagaaa cacgtttcat
    28381 cgaaaatccc gacattgtag ttttgagtgc gccattctga actgacgcat tgagaaaaac
    28441 tttgcgttat cgcagtttgt tgtaattttc tgaccactgc gccatatcca gaacagaact
    28501 atcagcagga acatcctgga gatcgtgcgt tacctgaacg acggggatcg ccagggcgcg
    28561 aacttcgctg aacacccact ggcgaaaatt atcgcgcaga gccacatcaa gacggctgaa
    28621 tggctcatcc aggagtaacg cttttggctg ggcgagaagg gcgcgtagta gagcaacgcg
    28681 cgctcgctga ccgccagaca aagtggcagg atcctgatgg aaagctccgt caaggcctga
    28741 acgctcaagt gcatcattca cggcattacg tcgggcattc cccttaagtg tcgccggtag
    28801 cgccagcagt aaattttgcc cgacgctgaa ctggtcaaat aacagtgcat cctgaaaaag
    28861 aatgccaatc tgacgctgtg cggtgggtag catgtcaatc cgttgctcat tgagccatag
    28921 ctcacctgta caagaaaact gtccggccag tgcaccaatc atccatgaaa acagagtgga
    28981 ttttccacag ccagacggcc ccattaacgt gacgatgtca cctttatcaa ccgtaaagtt
    29041 aacgtttgtc agcaagcggc tttctggtaa acgtagcgaa acatttttca cgcagagcat
    29101 tagcggattc cttgtctgac ataacctacc cattttgcga ctaacgcggt cagggcaaaa
    29161 ataataagcg gtaacaacag ttgccataac gcctgggcgg cgagaatacc gttgctgccg
    29221 ccgctgctta atgccaccgc ctcagtggtg agcgtcggaa aacgccccgc gcccagccac
    29281 agcgtcggca tatactgcgc aatactgact gaaaatccca ccgcgaaggc agtcagcaca
    29341 gggcgcaaca tgagtgggca tttcacgtaa aagaagattt tggcccgcga ccagcccagt
    29401 gtttgcgcaa tcaaaattaa ccgtgaatca atgcgctgcc aggcgggttg caggataaac
    29461 agcatccacg gcattaccca cagcagatgc ccccagacca ccgccgtcca gcttccatcc
    29521 aggttcagcc atagcgccag cgtgtactgg cccgccacca gtggcagagc gggcaataga
    29581 atgggcaacc atagccacaa ctggcgacgc tgtgcccccc attccagcca cagcagtaac
    29641 aggagcaaag cgatgaatgt tgctaccagc cccattgtca gactgttgat gagtgcttca
    29701 ctattgatcg tcgactgatc cgcgaggatc gccagcagaa ccacacacag cacaccggtt
    29761 aagggtaaaa aactcgccag cgtagtgcct ggtaataaag gtgtggcagg cttgcgaatg
    29821 ccatctacgc ggggaatagt gcgccgccag ctgcgccaaa acgtaggcgg cgagtaacag
    29881 catcagcagc agactggcga gtgcgccttt cgtttgttga tccgcgtcgc cctgagttaa
    29941 ccactgccag ctaattactg ccagcgtcgg tggattacct ggcccaagaa taatcgccac
    30001 atccacgacc gaaagcgacc aggcaacaat cgccagcatc gccattgcca gcgaaggcgc
    30061 gacggagggc aacagcagcc agttcaggca ttgccagcgg ctgtagccca gcgaatccag
    30121 cacaatgacc tgctgcaaca gccgtttttc gctcaatact gccactaaga tccacagcag
    30181 aaatgcgctt tctttcactg caagggtgag gcccagaccg atccaaatcg gtccattggc
    30241 ggagtgaaat acgggaaata gtcatatagc agccctccgt cagcaaagag cagcagggcg
    30301 ctggtggcaa aagccacatg gggaatggcg agcagccacg gcagacgggc gcacatacgc
    30361 tgccatttcg gcccaggcca tagcgccaca atcaccagca gggcaatcaa caatgccccg
    30421 accgccgcga tggttgtcga caccagcgtt gccagtaatg cctgcggtaa ctgtggatcg
    30481 gcaaataacg cctgccagcg tgtcaacgac aacgcaggcg atatcagcgt gagagctgcc
    30541 gggatcagcg gtgcataaat caccgccacc atcgcccaca gcaaaaagat taatgcgtac
    30601 cgtaacggcg taaccattct tgttccagtg cattcaccca tcctgcgtgc ggttcagcca
    30661 gtaccggcgg cagatcctgc ggcattcttg attgcaatat ttcgcgctgc ccgtcaggca
    30721 gtttttgcgg atcgagaaca gaaggatcgc cccagacaac gggatctgct ttacgcagtt
    30781 gcgcatcggg tgagagcagg aaattggcaa ctaccttcgc cgcagcactg gcattcgcgt
    30841 tggctggagt ggtgacgaaa tgcacgttgc caatcatccc ctcgcgaaag ccaaaactgt
    30901 aactgcttgc cggcaaatcg ccgctggcga ttttttgctg cgcatgtgcg gggttaaagg
    30961 tcagcgacag gcgcaatgtg ccggctttca gcagagcatc catccgcgcg ggcgaaggcg
    31021 ggaaatcctt tccttcgcgc cacaaatacg gatgcagtgc atcaagatat tgctacaagg
    31081 gcgcagtgac acgggcgaaa gtcgcatcgt ccggcgcttc ttttaatgcg gcgggatctg
    31141 gcgtcagcat aatcagcaac tgttcaagaa acgccgtgcc
//
LOCUS       DAJTUN010000037        30534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTUN010000037 DAJTUN010000000
VERSION     DAJTUN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.037"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1608
                     /locus_tag="OO470_001601"
     CDS             <1..1608
                     /locus_tag="OO470_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6250884.1"
                     /translation="SLMLEQNNVLIGENAWGKSSLLDALTLLLSPESDLYHFERDDFW
                     FPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTPCTDGYHRIFYRLEGESA
                     EDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLRDARFMRRIRNGTVPNVP
                     NVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEHYFSEQGAGQARYRLMRR
                     RASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQAKGTLRLDKDARPLLLIE
                     DPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVEHVCRLVRESSRVAAWRL
                     GPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWVINELARQCGHHFDAEGI
                     KVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATVRSLLNNDREAEREHLTA
                     LPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAIHRSSKPDLAIEVAMEAG
                     RRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1605..2561)
                     /locus_tag="OO470_001602"
     CDS             complement(1605..2561)
                     /locus_tag="OO470_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6250885.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2712..3827
                     /gene="macA"
                     /locus_tag="OO470_001603"
     CDS             2712..3827
                     /gene="macA"
                     /locus_tag="OO470_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6250886.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3824..5770
                     /gene="macB"
                     /locus_tag="OO470_001604"
     CDS             3824..5770
                     /gene="macB"
                     /locus_tag="OO470_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6250887.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5843..6067)
                     /gene="cspD"
                     /locus_tag="OO470_001605"
     CDS             complement(5843..6067)
                     /gene="cspD"
                     /locus_tag="OO470_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6250888.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6390..6710
                     /gene="clpS"
                     /locus_tag="OO470_001606"
     CDS             6390..6710
                     /gene="clpS"
                     /locus_tag="OO470_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6250889.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6741..9017
                     /gene="clpA"
                     /locus_tag="OO470_001607"
     CDS             6741..9017
                     /gene="clpA"
                     /locus_tag="OO470_001607"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6250890.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9420..9507)
                     /locus_tag="OO470_001608"
     tRNA            complement(9420..9507)
                     /locus_tag="OO470_001608"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9471..9473),aa:Ser,seq:gga)
     gene            complement(9761..9979)
                     /gene="infA"
                     /locus_tag="OO470_001609"
     CDS             complement(9761..9979)
                     /gene="infA"
                     /locus_tag="OO470_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6250891.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10264..10968)
                     /gene="aat"
                     /locus_tag="OO470_001610"
     CDS             complement(10264..10968)
                     /gene="aat"
                     /locus_tag="OO470_001610"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6250892.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11010..12731)
                     /gene="cydC"
                     /locus_tag="OO470_001611"
     CDS             complement(11010..12731)
                     /gene="cydC"
                     /locus_tag="OO470_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6250893.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12732..14498)
                     /gene="cydD"
                     /locus_tag="OO470_001612"
     CDS             complement(12732..14498)
                     /gene="cydD"
                     /locus_tag="OO470_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6250894.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14621..15586)
                     /gene="trxB"
                     /locus_tag="OO470_001613"
     CDS             complement(14621..15586)
                     /gene="trxB"
                     /locus_tag="OO470_001613"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6250895.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16130..16624
                     /gene="lrp"
                     /locus_tag="OO470_001614"
     CDS             16130..16624
                     /gene="lrp"
                     /locus_tag="OO470_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6250896.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16759..20787
                     /gene="ftsK"
                     /locus_tag="OO470_001615"
     CDS             16759..20787
                     /gene="ftsK"
                     /locus_tag="OO470_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6250897.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            20942..21553
                     /gene="lolA"
                     /locus_tag="OO470_001616"
     CDS             20942..21553
                     /gene="lolA"
                     /locus_tag="OO470_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6250898.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21564..22907
                     /gene="rarA"
                     /locus_tag="OO470_001617"
                     /gene_synonym="mgsA"
     CDS             21564..22907
                     /gene="rarA"
                     /locus_tag="OO470_001617"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6250899.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            22998..24290
                     /gene="serS"
                     /locus_tag="OO470_001618"
     CDS             22998..24290
                     /gene="serS"
                     /locus_tag="OO470_001618"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6250900.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24530..26974
                     /gene="dmsA"
                     /locus_tag="OO470_001619"
                     /pseudo
     CDS             24530..26974
                     /gene="dmsA"
                     /locus_tag="OO470_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            26985..27602
                     /gene="dmsB"
                     /locus_tag="OO470_001620"
     CDS             26985..27602
                     /gene="dmsB"
                     /locus_tag="OO470_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6250901.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27604..28395
                     /locus_tag="OO470_001621"
     CDS             27604..28395
                     /locus_tag="OO470_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6250902.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28431..29057)
                     /locus_tag="OO470_001622"
     CDS             complement(28431..29057)
                     /locus_tag="OO470_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6250903.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29372..30520
                     /locus_tag="OO470_001623"
     CDS             29372..30520
                     /locus_tag="OO470_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250904.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7514 a         7530 c         8241 g         7249 t
ORIGIN      
        1 tcgttgatgc tcgaacaaaa caacgtcctg attggggaga acgcgtgggg taaatccagc
       61 ttgctggacg ccttaaccct gctgctatcg ccagaatcag atctctacca ttttgagcgc
      121 gacgatttct ggttcccgcc gggagatatc aacgggcgag aacatcatct gcatattatt
      181 ttgaccttcc gcgaatcgct gccaggccga catcgggttc gccgttatcg gccgctggaa
      241 gcgtgctgga cgccatgcac cgatggctat caccgtattt tttatcgtct ggaaggggag
      301 agtgcggaag acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg
      361 attgatgttg aggatattaa cgatcaggca cgccatctgg tgcgtttaat gccggtgctg
      421 cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc
      481 aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg
      541 caaaatctct ctgatggaca gattcgtcag ggactttccg caatggtgca gttgcttgag
      601 cattatttct ctgagcaggg ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc
      661 agcaatgagc aacgaagctg gcgctatctg gacatcatca accggatgat tgaccgacct
      721 ggagggcgct cgtatcgggt tattttgctc ggcctgtttg ctactttatt gcaggcaaaa
      781 ggcacattgc gactggataa agacgcgcgt ccgttattgc taattgaaga tccagaaacc
      841 cgcctgcatc cgattatgct ttccgttgcc tggcatctgt tgaatctttt gcctttacag
      901 cgtattgcta ccaccaactc tggcgagctg ctttcgttaa ctccagtgga acatgtttgc
      961 cgcctggtgc gtgaatcttc tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc
     1021 accgaagaca gccgccgcat ctcttttcac attcgtttta atcgtccgtc atcgctgttt
     1081 gctcgctgct ggttgctggt ggaaggggaa acggaaacct gggttatcaa tgaactggcg
     1141 cgtcagtgcg gacatcattt tgatgccgaa gggatcaagg tcattgagtt tgcccagtcc
     1201 gggctaaagc cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc
     1261 gatggcgatg aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataacgat
     1321 cgggaagccg aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg
     1381 tatcgccagg gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg
     1441 atgaatctac gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc
     1501 attgaagtgg caatggaggc aggacgtcgt ggtgtggact ccgtaccaac gctgctgaaa
     1561 aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc
     1621 gtggccattt gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt
     1681 tttttactgg cgatttcggc aatctctttt cgtgctatct gtgctggaag gcggtaatgg
     1741 cgttcagcat cacatacgcc gccaaccgac tcccagaacg cgttgtaatc agcatggatc
     1801 ttgccttctt tatcgcgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca
     1861 ataatctgct ctacctgcaa acgttgggca aacagacagg ccgcttccat cacgaggcgt
     1921 ttgggaaata gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc
     1981 cattttgcgc cttgcagtcc gccaataaac atcgttcttt tcccctgata ttcacacagg
     2041 gtaaacgtga tctctgccag aggaatacct tcgctgttgc ggaacaggat tgtgctgtca
     2101 ccttctttat ccattgagat catcatggtc agctcaagcg tgaactgctc accgtttttg
     2161 ccttccagtt tagccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc
     2221 gacatacacc cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg
     2281 ctaagattcg cggcaaggta agggcgatgc agacgcacag gcagtcgcgg ctggcgcgtt
     2341 aacaacacat tgagattagg ccagtgggaa agttcgttca tccactcaac gcttaaacgc
     2401 ggcataatca acgagcgcag caaaaatttc tggcgaaaac tacagcgatg ccagaattta
     2461 cccggccgac actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca
     2521 gatggcgtaa aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat
     2581 gacgtcgatt tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaacgtaa
     2641 attttagggt tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat
     2701 tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga
     2761 tagtcatcgc cggactgatt acgttatgga gaattcttaa cgcacccgtg ccgacttatc
     2821 agacactgat tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg
     2881 acgcgctgcg taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa acgctgtcgg
     2941 tggcgattgg cgataaagta aaaaaagacc agcttttagg ggtcattgat cctgaacagg
     3001 ctgaaaacca gatcaaggag gtcgaagcaa cgctgatgga gctacgcgcg cagcggcagc
     3061 aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac
     3121 aaacgcaagc ggtttcactg caggatctcg ataccgccgc gacggagatg gctgtgaaac
     3181 aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcctct ctcgatacgg
     3241 ctaaaaccaa tctcgattac acccgtatcg ttgccccgat ggctggggaa gtgacgcaaa
     3301 tcaccactct gcaaggccag acggtgattg ccgcacagca agcaccgaac attctgacgc
     3361 tggcagatat gagcactatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc
     3421 tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga tccactgacg cgctacgagg
     3481 ggcaaatcaa ggatgtgcta ccgacgccgg aaaaggtaaa cgacgctatt ttctattacg
     3541 cccgttttga agtacccaac cccaatggtt tgctgcggct ggatatgacc gcgcaagtgc
     3601 atattcagct caccgatgtg aaaaatgtgt tgacgatccc tctgtcggcg ttaggcgatc
     3661 cggttggcga taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg
     3721 aagtgacgat tggcgcacgt aacgataccg atgttgagat tgtcaaaggg ctggaagcgg
     3781 gcgatgaagt ggtgattggt gaggccaaac caggagctgc acaatgacgc ctttgctcga
     3841 attaaaggat attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg
     3901 catcagcctc aatatttatg cgggtgagat ggtcgcgatt gttggcgctt cgggttccgg
     3961 taaatcgacc ctgatgaata ttctcggctg cctggataag gccaccagcg gcacctatcg
     4021 cgtagccggt caggatattg ccacgctgga cgccgatgcg ctggcgcaac tgcgccgcga
     4081 gcatttcggc tttattttcc agcgttacca tttgcttccg catttaaccg ccgagcagaa
     4141 cgttgaagta cccgccgtct atgccggtct tgagcggaaa cagcgactgc ttcgtgccca
     4201 ggagttgctg caacggctgg ggctggaaga ccgtacagag tattatccgg cacagctttc
     4261 gggtggtcag caacagcgcg tcagcatcgc gcgggcattg atgaacggcg gtcaggtaat
     4321 tcttgccgat gaaccgaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat
     4381 cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc gtcacccacg atccgcaggt
     4441 cgctgctcag gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc
     4501 cgccattgaa aaagtgaatg ttgctggcgg gacggagcct gttgtcaata cagtgtctgg
     4561 ctggcggcag tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcgctggc
     4621 agcgaataaa atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt
     4681 ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc
     4741 tattggtacg aatactattg atgtctatcc cgggaaagat tttggcgatg acgatccgca
     4801 atatcagcag gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc
     4861 ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc
     4921 tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga
     4981 aggaaacacc tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag
     5041 taatactcgc cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt
     5101 cggcaatatg cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag
     5161 cagtaaagtg ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca
     5221 gtcgtggctt aactccatta ctgtcagggt gaaagaagga tttgacagcg ccgaggcgga
     5281 acagcaactc acgcgtttac tttcactgcg ccacggtaag aaggatttct ttacctggaa
     5341 catggacggc gtcttgaaaa ctgttgaaaa gaccacacgt actttacaac tgtttctgac
     5401 gctggtggcg gtgatttcgc tggtggtggg cggtattggt gtaatgaata ttatgctggt
     5461 gtcagtgacc gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag
     5521 cgatgttttg caacagttcc tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt
     5581 gggaataaca ctgtcactgt taattgcttt caccttgcag cttttcttac ccggctggga
     5641 gattggtttt tcaccgttgg cgctgctgct ggcgtttctc tgctcgacgg ccaccgggat
     5701 tttatttggc tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc
     5761 acgggagtaa tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga
     5821 tgtacacaat gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa
     5881 cactggcgtg attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt
     5941 ttagcgttct gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc
     6001 cgccttcagg gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct
     6061 tttccatgct tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc
     6121 agagaggggg ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg
     6181 agccgtcaag catttgacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt
     6241 aacgctattg acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttatct
     6301 atcatgtgga gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg
     6361 ataaccgtaa ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg
     6421 atcaactggc ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga
     6481 tattagtcaa tgatgattac actccgatgg agtttgttat tgacgtgtta caaaaattct
     6541 tttcttatga tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg
     6601 ccatttgcgg agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt
     6661 acgcgaggga gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat
     6721 aaaaattggg ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt
     6781 tcgccagagc gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc
     6841 tgctcagtaa cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc
     6901 tccgtcagga actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag
     6961 aggagcgcga cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc
     7021 atgtccagtc ctccggtcgc aatgaggtta ccggtgcaaa cgttctggtc gctatcttca
     7081 gcgaacagga gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg
     7141 tggtgaactt tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg
     7201 gcagccagcc aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga
     7261 cgaacctgaa ccagcttgcg cgtgtgggcg gaatcgaccc actaattggt cgtgagaaag
     7321 agctggagcg tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg
     7381 gggaatctgg tgtcggtaaa accgcgattg cagaaggtct tgcctggcga attgttcagg
     7441 gtgatgtgcc ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt
     7501 tagcgggcac aaaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc
     7561 tggagcagga cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg
     7621 gtgcagcgtc tggtggtcag gtcgatgcgg ctaacctaat caaaccgttg ctctccagcg
     7681 gtaaaattcg cgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag
     7741 accgtgctct ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtcg atcgaagaaa
     7801 ctgttcaaat catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata
     7861 ccgcaaaagc ggtgcgtgcg gcggtagagc tggcggtgaa atacattaac gatcgtcatc
     7921 tgccggataa agccattgat gttatcgacg aagcgggcgc tcgcgcacgt ctgatgccgg
     7981 taagcaaacg caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg
     8041 cacgcattcc agagaagagt gtttctcaga gtgaccgcga taccctgaaa aaactcagcg
     8101 atcgcctgaa aatgctggtc ttcggccagg ataaagccat tgaggcgctg actgaagcca
     8161 ttaagatggc gcgtgcaggt ttaggtcacg aacataaacc ggtcggttcg ttcctgtttg
     8221 ccggtcctac cggggtcggg aaaacagagg tgacggtaca gctttcgaaa gcgttgggca
     8281 ttgagcttct gcgctttgat atgtccgagt atatggaacg ccataccgtc agccgtctga
     8341 ttggtgcgcc tccgggatac gttggttttg atcagggcgg tttgctgacc gatgcggtaa
     8401 tcaagcatcc acatgcggtg ctgctgctgg acgaaatcga gaaagcgcat ccggacgtgt
     8461 tcaatattct gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag
     8521 cggacttccg taacgtggtg ctggtgatga ccactaacgc cggggtacgg gaaactgagc
     8581 gcaaatccat tggtcttatc caccaggata acagcaccga tgcgatggaa gagatcaaga
     8641 agatctttac accggaattc cgtaaccgtc tcgacaacat tatctggttc gatcatctgt
     8701 caactgacgt gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagctgg
     8761 atcagaaagg tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag
     8821 gttacgaccg ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa
     8881 aaccgctcgc caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtgacggttg
     8941 cgctggataa agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg
     9001 cggaagcagc gcattaatct gattgtcagg taggttggtg aagtccgtaa tctcgtcagg
     9061 ggttacggac tttttattta tggggtggaa cggtgaaaac cctatttttg gaggtgaagg
     9121 taagttgttg ataattagtg ctgctggaag gtaaggataa aaaagggtgc tgcaggagaa
     9181 tgggatggtt ttgctttatt aacaacgggc taaacgtgta gtatttgagt tcactgccgt
     9241 acaggcagct tagaaatcga cggggtgcgg taaaaccttt gcgaacgcgt tcactgccgt
     9301 acaggcagct tagaaattca caggtaacat actccacccg cccaccatgt tcactgccgt
     9361 acagacagat aaaatgcgaa aaaaaagctc gcactttcgt acgagctctt ctttaaatat
     9421 ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt tccaggcgtg
     9481 ctccttcagc cactcggaca cctcaccaaa ttgttttgct accaaacctc atgggtggca
     9541 acggggcgct actataggga gttggagtaa aacggtcaag aagaatttta atgataatta
     9601 ttgtttgctc atactgtaaa caacttgtgc agtatatcta catcgagaca agttatggac
     9661 ttatacttcc aaagtacttc atacatatca caaaataaaa aggccggtta aaccgacctt
     9721 ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa tcagcgacta cggaagacaa
     9781 tgcggccttt gctcaggtcg tacggggtca gttcaacagt cactttgtcg cccgtcagga
     9841 tgcggatgta gtttttgcgc attttaccgg agatgtgtgc agtaaccacg tgaccgtttt
     9901 ctaactctac gcggaacatg gtattaggca acgtttcaag aacggtacct tgcatttcaa
     9961 tattgtcttc tttggccatc taatcctctg gggtatcact accgtaattt gaaccggcaa
    10021 gataatgccg aagttctgta aataagtaaa gatttgcgcg ctaaatcgca acaaacaggt
    10081 tcggcacatt actccgaaaa cacacggcta agccgcacca aaagcgcaac gtataaggga
    10141 gcggtgagat aaacgatggg cgttacctga cgcgaaaaat tccttatcgg cagcggggta
    10201 atgagcgtaa ccaactctgc gaccgcaatt ataacactct ggggagaaat gtgccgaaaa
    10261 cattcattct tgtggtgaaa acaagcaccg tggtacccag aaattattcg gcaatcgtcc
    10321 gaggcgcatt tgattgagat aattaaggta atcccggcgg ggaatttcgc aggcaccaag
    10381 cgatgctgtg tgatcgttaa ggacctggca gtcgataagc ttaccgccat gaccgataaa
    10441 ttcctcacag aataccagaa gcgccgtttt agacgcattt tccatccggc tgaacatgga
    10501 ctcgccacaa aatagcgttc cctgggccac gccgtacata ccgccgacaa gctcatcttc
    10561 acgccagact tcaatggagt gggcatgacc gagttcgtga aggcgatggt aggcttcgac
    10621 cacgccacgc gtgatccagg ttccttcttc gcgatcgctg gcacagcctt caatgacctg
    10681 accaaaagcg taattcatcg tgacacgata gggcgagcgt ttatgaaatc gcttcatact
    10741 acggctgata tgcagtgatt ctggccatag caccgcgcgg ggatcgggcg accaccagag
    10801 gatggggtcg cctggagaaa accacggaaa aataccacgc tggtaagcca ttaacaggcg
    10861 cgcagggcta agatcgcccc caagtgccag caggccgtta ggctcacgta atgcgccttc
    10921 cggggaaggg aaggctattg aatggcgaga aagctgaacc aagcgcatga ccgcaaaact
    10981 ccacgcaagt cggatcgttc aataatagct tacaaaccct gcttgaactg gtaataacgc
    11041 ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa taatttgccc gttgtccatc
    11101 actattattt gttggaaacg agagagtccg cgaagtcgat gggtgaccat taacaccgtt
    11161 ttctcacgca tcatttttgc aagcaattca aggatctggc tttcggttgt ggcatctaag
    11221 ccttcggtag gttcatccag caacaccagt ggcgcatcat gtaacagcgt acgggcgata
    11281 gccagacggc gcagttcacc accggagagc tggcgtccgc cttcacctaa ccaactgttg
    11341 agacctgcat cctcgagcag cttctccaga ccaacgcgac gcaagatctc cgacagagcc
    11401 tcatcactac tgccaggcga ggcgagtaac agattatcac gcagcgtggc gctaaacaga
    11461 tgcactcgct gaggaacaac gctgatggtc tgtcgtagag ccgcttcatt caggctggct
    11521 atggggctat cgttaagcaa aatctcgccc tgttgcgggt cccatgcgcg ggtcagcagt
    11581 tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa tcgctatatg ttccccggcg
    11641 tttacctgaa gagaaatccc tttaagtgcc tgttgggatt gctccggata agtgaactga
    11701 acatcccgta atgtcagcga aacgcgatcg gcaacacgag tttgggtatc agggaaggtg
    11761 acctccggtt tttgatccgt taagtcagag atacgtacgg cagaggcaat gacttgcccc
    11821 agatgctgaa atgcacccgt tactggtgcc agtgcttcaa acgcggctaa cgcacaaaag
    11881 acaaacaggg caattaatgc gccgggttga gcattgccgc caacgccgcc agacgccatc
    11941 cacagcatca ggatcaccgc taacgcgcca atgagcagca ttatcgcttg cgacaatgcg
    12001 gtcagttcag attgacggcg ttgcgcttcc agccattgaa tttctgtatt ctctagttgc
    12061 gtgcgataac gatcgctggc accaaaaatg gtcagctcag cttgcccttg cagccaggcc
    12121 gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat tttgcccggt gctttttccc
    12181 gcacgataaa acagcggtgg catcaggaaa agcgtcagta acataacgct gcccagcgta
    12241 aaggcgaggg tgaaatcaag gaaacttaac ccgattgtca ccaccataat caccacaaaa
    12301 gcgcccacca gcggcgagat aacgcgcagg taaagatgat cgagcgtatc aacatccgcc
    12361 accacgcggt tgagcaattc gccctgacga tagcgcgcca gtccggcagg ggagaggggc
    12421 aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca cgcggaaagt cgcgtcgtga
    12481 cttaccagac gttcaaaata acgcccggca gtacgggtga ttgcggcacc acgcacgccc
    12541 gcagcgggta gcatatagtt gaagctgtac agtccggcaa cccccgcaac cgctgaggcc
    12601 gagaggaacc agccggaaag tgtcaacaga ccgatactgg cgagcagcgt cacaattgcc
    12661 agcacaatac caagacttaa catccattta tgacgtttat acagtgccag atagggtagc
    12721 aaagcgcgca tttaaatctc ctcctgacga tgggccagta atgtggcgaa tgggccacca
    12781 gccacactta attccgcgta acgtccttgc tcaataatcc ggccatcctg cataacccaa
    12841 atgacatccc agtcagcaag atcttctaac tggtgggtga ccattaacgt tgtctggcgc
    12901 agagaggcgg cattcagcgc ctccattacg cgctgttcac tgtgagcatc aaggctggca
    12961 gcgggttcat ccaacagtaa tagcgaacag ggatttagta acgcacgggc caccgccacg
    13021 cgctgcgcct gccccacgga aaggcgggca gcctggtcgc caacaggtgt atcaacgcct
    13081 tgtggcagga gcggtagaaa ctcgctgacc caggcgttat ccagcgctgc ttgtaattct
    13141 tgttcgctgg catcaggtcg cgccagtagt acgttatccc gcaattttgc tgccggtaat
    13201 tgtgggtttt gcccaaccca ggagagatgt ttacgccatg attctggtga taaatcacgt
    13261 aattctatcc cgttgattcg taatgatccc tgatatgaga gaaaaccaga aagcgcgttc
    13321 agcagtgagc ttttacctga accgctgcgg ccaaccaaca ctgcacgttg gcctgctggc
    13381 aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg gcgacgtgat aaacagatcc
    13441 tcggcctcaa tggtcagcgg atcggtcaat gctaattccg cctcaccgcg ctgcggatgg
    13501 gcgagcgggg tttccataaa cgttttcaga ctgtcagctg caccaacagc ctgggcttta
    13561 gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa actctggcgc aaggatcagg
    13621 gccagaaaac ctgcagccag cgtcacccca gtatcgtagt gaccaaaatc cagctcgccg
    13681 agataggaaa aaccaaagta gaccgccacc agagcaattg acagcgaggt aaaaaattcg
    13741 agaatgccgg aggataaaaa cgccagtcgt agcacttcca ttgtccgttg gcggaaatct
    13801 tccgaagcag aacgaatact ttcaatttca gcttcaccac gaccaaaaat acgcaatgtt
    13861 tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc gagcaagagc gagaaagtta
    13921 cgccggttag catcggcagc ccccattcca accagcgcca taaacaacgg aattagcggt
    13981 gctgtgccca gcagaatgag cgccgcagcc cagttagagg ggaagatcgc caccacaatc
    14041 agcaacggca ccgacactgc cagcgccatt tgcggcagat agcgtgcata gtaatcatgc
    14101 atatcgtcaa tttgctcgag taccagcgtc gcccagctcc ccgcaggttt accctgaatc
    14161 cacgctggcc ctgcttgttg cagacggtcg agaacctgac ggcggatggc aaagcggata
    14221 tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga ccacccatgc gcgcaataca
    14281 aaggtcagaa ccagtaacgt aaagggaagc agcagggctt cacggggaat attctccata
    14341 atcatatgtt gcagaattcg cgccatgaac caggcctggg caatgatcaa tatgccgctt
    14401 acaaagccca gcagacgaga aatattcagc caacgttggg agatgacgct ttgctgtttt
    14461 aaccagcggg ttaactcttt ttgacgagat ttattcattg cacgcttagc aggtgagtta
    14521 tcagaattat ttgcagagca atgttacaac ggggaaaaaa taaaggcgac ccatagtcgc
    14581 aaggtgtcgc cttctttact tttgttactg atttgtaaaa ttattttgcg tcagctaaac
    14641 catcgaggta gcgttccgca tcaagtgctg ccatgcagcc agtaccggcc gaagtaatgg
    14701 cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa gacgccagga atgctggtct
    14761 gggtggcatt accatgaata cccgactgta ctttgatgta gccgttttcc agttccagct
    14821 gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc aacaaacaga ccggcaacgt
    14881 cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag acgaacgcca gtgacaccca
    14941 tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg cagaatgatg ttgccgttct
    15001 ccactttatc catcagacgc ttaataagga ttttttccgc gcggaaaccg tcacggcggt
    15061 gaatcagatg cacttccgaa gcgatgttag acagatacag cgcctcttca accgcggtat
    15121 tgccgccgcc gatgaccgca actttctggt tgcgatagaa gaaaccgtcg caggttgcac
    15181 aagcagaaac cccacggcct ttaaaggctt cttcagaggg caggccgaga tagcgtgcag
    15241 aagctccggt ggcaataatc agcgcgtcgc aagtgtattc ggcgttatcg ccattcagac
    15301 ggaacggacg gttttgcaga tccaccttgt tgatatgatc aaaaatgatc tcagtttcaa
    15361 acttggtggc atgttcgtgc atgcgcgcca ttaataacgg accggtcaga tcgtttggat
    15421 caccaggcca gttttccact tccgtggtgg tggtcagttg gccgcctttt tccatgccgg
    15481 taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac agcagcggtg tatcccgccg
    15541 ggcctgaacc caggataagc agtttactgt gtttggtcgt gcccatgaga tccccatagt
    15601 tgttggcaga caatgggcag gattgtaggg aatttacaga cgtaaaaaaa gagtatgacg
    15661 attttgttaa caatttgtgc aatcggcagc atcgataagc aggtcaaatt ctcccgtcat
    15721 tatcacctct gctacttaaa tttcccgctt tataagccga ttaaatgatg aataaacgcc
    15781 cttgttaatg aatatctggc atgttgtact aaaaatcgat gttttgcttt gacaatcccc
    15841 tagtgttttg cgaaaacatt cgaggaagaa aaaaacagta ttcttatatg cgcataacca
    15901 tgtatgtaaa taccatgttt accgtgctag tgaaatctac gtatggcgtg gacagacgcc
    15961 attcgtgatg tcgatagctg ccacaaggca acggtcttct caccgtagac ccaggcattg
    16021 cgcgccgtga atcttcatga tttcggtcta tcgtgacggg tagcgactct gaacagtgat
    16081 gtttcagggt cagacaggag tagggaagga atacagagag acaataataa tggtagatag
    16141 caagaagcgc cctggcaaag atctcgaccg tatcgatcgt aacattctta atgagttgca
    16201 aaaggatggg cgtatttcta acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc
    16261 gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt attcagggct atacggcgct
    16321 gcttaacccc cattatctgg atgcatcact tctggtattc gttgagatta ctctgaatcg
    16381 tggcgcaccg gatgtgtttg aacaattcaa taccgctgta caaaaacttg aagaaattca
    16441 ggagtgtcat ttagtatctg gtgatttcga ctacctgttg aaaacacgcg tgccggatat
    16501 gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac
    16561 acggacatac gttgttatgg aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg
    16621 ctaacacgga acaggtgcaa aatcggcgta ttttgattac actcctgtta atccatacag
    16681 caacagtact ggggtaacct ggtactgttg tccgttttag catcgggcag gaaaagcctg
    16741 taacctggag agcctttctt gagccaggaa tacactgaag acaaagaagt cacattgaca
    16801 aagttaagta gcggccgccg ccttctggag gcattgctga tccttattgt cctgtttgcc
    16861 gtctggttga tggctgcctt actaagcttt aacccttcgg accccagctg gtcgcaaacg
    16921 gcctggcatg aacctatcca taatttaggt gggatgcccg gtgcgtggtt ggcagatacg
    16981 ctgttcttta tttttggcgt gatggcttac accattcccg tcattattgt cggcggttgt
    17041 tggtttgcct ggcgtcatca gtccagcgac gaatacattg attattttgc cgtttcgcta
    17101 cgcatcattg gcgttttggc gctcatcctt acctcctgtg gtctggcggc aatcaacgct
    17161 gacgatatct ggtattttgc ctccggtggc gtcattggca gcttactaag cactacgcta
    17221 caaccactgc tacacagtag cgggggaact attgcgctgc tctgcgtttg ggcagcgggc
    17281 ctgacgttgt tcaccggttg gtcatgggtg accattgctg aaaaactcgg cggctggatt
    17341 ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa
    17401 gatgagtatg aagacgacga agagtatgaa gatgaaaatc acggcaaaca gcatgaatca
    17461 cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc ggaaaaattc
    17521 attaatccga tggggcggca aacagacgct gcgttgttct ccggtaagcg gatggatgat
    17581 gacgaagaga ttacctacac tgcacgcggt gtggctgccg acccggacga cgtcctattt
    17641 tcgggcaatc gtgcaacgca gccagaatat gacgaatacg atccattatt aaacggtgcg
    17701 ccaattaccg aacctgtcgc tgtagcagct gctgctacca cggcgacaca aagctgggct
    17761 gcgccggttg aacctgtgac tcagacgccg cctgttgcct ctgttgatgt tccaccttcg
    17821 caacctacag tagcctggca gcctgtaccg ggtccacaaa cgggagagcc ggttattgct
    17881 cctgcaccgg aaggttaccc acagcagtca caatatgcgc agcctgcagt gcaatataat
    17941 gagccgctgc aacaaccagt acagccgcag cagccgtatt atgcacctgc agctgaacaa
    18001 cctgcgcaac agccgtatta tgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18061 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18121 tatcagcagc cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18181 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccgctttac
    18241 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18301 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18361 tctgttgcag cagtacctcc agtagaagcc gctgccgctg tttctccgct ggcatctggc
    18421 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18481 ctggcaaatg gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18541 cgaccgaaac gtatccgcgt gccaactcgt cgtgaattgg cgtcttacgg tattaagctg
    18601 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtatgattct
    18661 ggcgatcagt ataacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    18721 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    18781 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgt gcaaactcaa
    18841 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    18901 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgct gtttacgcca
    18961 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cacagcagca gtatcagcag
    19021 ccacaacagc cggttgcgcc acagccgcag tatcagcagc cacaacagca ggttgcgccg
    19081 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19141 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcaccgcag
    19201 ccgcagtatc agcagccaca acagccagtt gcgccacagc cgcaggatac cctgcttcat
    19261 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcaca aaccgacgac gccgctgcct
    19321 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19381 gaacaaatgg cgcgtctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtc
    19441 gttaattact ctccggggcc agttatcact cgctttgaac ttaacctggc tcctggcgta
    19501 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19561 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accaaataaa
    19621 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    19681 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    19741 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    19801 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    19861 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcacct gttaacggaa
    19921 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    19981 cgtcggtata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20041 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20101 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtcgt gttggttgac
    20161 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agctcgtctg
    20221 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20281 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20341 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctgggtatg
    20401 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20461 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20521 tatgttgatg gcatcacctc cgacagcgaa agcgaaggtg gtgcgggtgg tttcgatggc
    20581 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    20641 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    20701 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    20761 gtgctggccc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    20821 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    20881 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    20941 gatgaaaaaa attgctatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21001 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21061 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21121 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21181 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21241 ctggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21301 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21361 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21421 gtttagcgcg gtggagcagg acgatcagcg cagcagttat cagctgaaat cccagcaaaa
    21481 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21541 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21601 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggtcagcaac
    21661 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ttgcattcta
    21721 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    21781 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    21841 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    21901 acgaagttca ccgtttcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    21961 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22021 tgctttcccg tgcccgtgtt tatctgttga aatccctgag tacagaggat attgagcaag
    22081 tactaactca ggcgatggaa gacaaaaccc gtggctatgg tggtcaggat attgttctgc
    22141 cagatgatac acgacgtgcc attgcagaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22201 atacgctgga aatgatggcg gatatggccg aagtctatga tagcggtaag cgggtcctga
    22261 agcctgaatt actgatcgaa atcgccggtg aacgtagcgc ccgctttgat aacaaaggcg
    22321 atcgctttta cgatctaatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22381 cggcgctgta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22441 gtcgctgtct ggcgattgcg tctgaagacg tcggtaatgc cgatccacgg gcgatgcagg
    22501 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22561 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22621 ttaaagccgc gctggccgat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    22681 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    22741 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    22801 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggtgaa aagctcgcct
    22861 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    22921 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    22981 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23041 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23101 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23161 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23221 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23281 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23341 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23401 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23461 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23521 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23581 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    23641 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    23701 tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg
    23761 atctgccaat taagatgatc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    23821 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    23881 tcgtgcgccc agaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    23941 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24001 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24061 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24121 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24181 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24241 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24301 ttctgaatct aaaaagcgcc tttagggcgc tttttttgtc tccctttgat accgaacaat
    24361 aattactcct cacttacacg taatactact ttcgagtgaa aatctaccga tctctttgat
    24421 tttcaaatta ttctatgtat acaagcctat atagcgagct gctatagaaa taattacaca
    24481 atacggtttg ttactggaat caatcgtgat caagcttgag tgagccatta tgaaaacgaa
    24541 aatccctgat gcggtattgg ctgctgagga gagtcgccgt ggtttggtaa aaacgacagc
    24601 gatcggcggc ctggcaatgg ccagcagcgc attaacatta ccttttagtc ggattgcgca
    24661 cgctgtcgat agcgccattc caacaaaatc agacgaaaag gttatctgga gcgcctgtac
    24721 agttaactgt ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg aaatcaaata
    24781 tgtcgaaacg gacaataccg gcgatgacaa ttacgacggc ctacactagg ttcgcgcctg
    24841 cctgcgtggg cgttccatgc gtcaccgtgt ctacaatccg gaccgcctga aatatccgat
    24901 gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg aagaagccta
    24961 cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg agtctatcta
    25021 tctgaactat ggcaccggta cgctgggcgg caccatgacc cgctcctggc cgccgggaaa
    25081 taccctggtc gcgcgcctga tgaactgctg cggcggttat cttaaccatt acggtgacta
    25141 ctcttccgcg caaattgcgg aaggtttgaa ccatacctac ggcggctggg cagatggtaa
    25201 cagcccgtcg gatatcgaaa acagtaaatt ggtagtgctg tttggtaata acccaggcga
    25261 aacgcgaatg agtggcggtg gggtgactta ctatcttgaa caggcacgcc agaaatctaa
    25321 tgctcgcatg atcatcatcg atccgcgcta taccgacacc ggtgccgggc gcgaagatga
    25381 gtggatccct attcgtccag gaacagatgc cgcactggtt aacggtctgg cgtacgtcat
    25441 gatcattaaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg gctatgacga
    25501 gaaaaccctg cccgccagtg cgccgaaaaa tggtcattat aaagcttata ttctgggtga
    25561 agggccagat ggcgtggcta aaacaccgga atgggcctcg caaatcaccg gtgttccggc
    25621 agacaaaatc atcaaactgg ctcgtgaaat cggcagtacc aaaccggcgt ttatcagcca
    25681 gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta tctcgatgct
    25741 ggcgattctg accggtaacg ttggtattaa tggagggaac agcggcgcgc gtgaaggttc
    25801 atacagctta ccgtttgtcc gtatgccgac cttggaaaac ccgatccaga ccagcatttc
    25861 gatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacggcgc tgcgtgatgg
    25921 tgtgcgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact atgccggtaa
    25981 ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat gaaatcctcc aggatgataa
    26041 gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcggcga aatatgctga
    26101 catcctgctg cctgactgca ccgcttccga acagatggac tttgcactgg atgcatcctg
    26161 cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct ttgaatgtaa
    26221 gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc aacagtttac
    26281 tgaaggccgt acctaggaag agtggatgcg gcatctgtat gcccagtcgc gggaagcgat
    26341 tcctgaactg ccaacgtttg aagagttccg caagcagggg atctttaaaa agcgcgaccc
    26401 acaagggcat cacgttgctt ataaagcctt ccgtgaagat ccgcaggcaa acccactgac
    26461 tacgccatcg ggcaaaattg agatttattc gcaggcgctg gctgacattg ccgctacctg
    26521 ggaattgcca gaaggcgatg tgatcgatcc actgccgatc tacacgccgg gctttgaaag
    26581 ttatcaggat ccgctgaaca aacagtatcc gctgcagctt acaggtttcc actataaatc
    26641 tcgcgttcac tcaacttaca gcaacgttga tgtgctgaaa gcggcttgcc gtcaggaaat
    26701 gtggatcaac ccgcttgatg cccaaaaacg cggtatccac aacggcgata aagtcaggat
    26761 ctttaacgat cgtggtgagg ttcatattga ggcgaaagtg acgccacgaa tgatgccggg
    26821 cgtggtcgca ctgggtgaag gcgcctggta tgacccggat gcaaaacgtg tcgataaggg
    26881 cggttgtatc aacgtactga ccactcaacg tccgtctcct ctcgctaagg ggaatccgtc
    26941 acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca acccagtatg
    27001 gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg gcttgtaaag
    27061 actacaaaga tttgacgcca gaagtcagtt tccgccgcat ttatgaatat gctggcggcg
    27121 actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat ctgtcgattt
    27181 cgtgcaacca ctgcgaagat ccggcttgta ctaaagtctg cccgagcggt gcgatgcata
    27241 aacgcgaaga tggttttgtt gtggtcgatg aagatgtatg cattggctgc cgttactgtc
    27301 atatggcttg cccgtatggc gcaccgcaat ataacgaaac gaaaggccat atgaccaaat
    27361 gcgatggttg ttatgaccgt gttgcggagg gtaaaaagcc gatctgtgtt gaatcttgcc
    27421 cgctgcgggc gctggatttc ggccctatcg acgagctgcg taaaaaacat ggcgatctgg
    27481 cggccgttgc gccgttgccg cgagctcact ttaccaaacc gaatattgtg atcaaaccca
    27541 atgccaatag ccgcccgacc ggggatacca ctggctatct ggcaaacccg aaggaggtgt
    27601 gagatgggaa gtggatggca tgaatggccg ctgatgatct tcacggtctt cgggcaatgt
    27661 gtggcgggtg gttttatcgt tctggctttg gcgctgctca aaggcgacct gcgagcagaa
    27721 gcccagcagc gtgttatcgc ctgcatgttt ggcttatggg tactgatggg cattggcttt
    27781 atcgcctcta tgctccatct tggttcaccg atgcgcgcct ttaactcgct caaccgggta
    27841 ggggcttcag cactcagtaa cgaaatcgcc agcggttcga tcttttttgc tgtaggcggc
    27901 atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg cattgcgtac gctatggctg
    27961 gtggtgacga tggttcttgg cgtcgtcttt gtctggatga tggtgcgtgt gtataacagc
    28021 attgataccg ttccgacctg gtacagcatc tggacgccga tgggctgggc gatgcgtctg
    28081 ctgccagcca tttctgtact ggcactggtg gtgagtggcg tgatgtcagt gatgcagggc
    28141 acagagctgg caactattca tagttctgtg cagcaggccg cagcgctggt gccggactat
    28201 ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg ccctgtgctt gtggattgca
    28261 ccacagctaa aaggttatca gcctgcggtt ccgctactat ccgtctcatt cattctgctt
    28321 ctggcagggg aattaatcgg tcgcggcgta ttctatggtt tgcatatgac cgtggggatg
    28381 gccgtcgcaa gctaatcagt aacaaccggg gtttcggccc cggttctctt ttatttctgc
    28441 ttcgttaacg tgtcataact ggtcatcaaa ttacgataat caggaatatg gttagagaac
    28501 aacgtcgcca gtccttcaat atcattacgc cagtcacggt gcagctcgca agccacgccg
    28561 aaccatgtca tcaattgcgc accagcttgc gacatgcgat cccacgccga atgccgggta
    28621 atttcattaa aggtccctga tgcatcagtc acgacaaaga catcaaaccc ttcttcaatg
    28681 gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat aattaactgt
    28741 tttttacctg tcgctttgac tgcttttaca aaatcttcgt tatcccaggc gttaatattg
    28801 cccgggcgag caatgtaagg tgcatcgggg aattgtgctt tcagttctag aactaatggg
    28861 ccgttaggac cggtttcaaa actggtggta agaatagttg gtaagttgaa atacttagcc
    28921 aggtcaccca gcgccagcac gttattttta aacttatcgg gttcgatatc ccgtacaagg
    28981 gaaagtaaac cagcctggtg atcaacaagc aaaacggcag catcattttt atcaagacga
    29041 acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgtct gaaatcggtc
    29101 agacgcgatg cattgctctg aaagcataga cgggaaatat gagtttgctg tgaccatgaa
    29161 atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac aatcagatga
    29221 gattaatcat ctcttcagca ttagcgttat gaatgagtaa tataaatcga taataccgcc
    29281 gctgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc atgatgcgca
    29341 tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc ctgtcatgct
    29401 tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata cactcgtgcc
    29461 gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca gctcatccta
    29521 ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaaac gcattggctt
    29581 taaccgcagc tattatctgt cctccttcat ttttgctgct ggctgtgccg gccttggcct
    29641 gatgattggt ttctggagct ggttggcgtg gcgttttgtc gcgggcgtcg gctgtgccat
    29701 gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta accgtgggcg
    29761 tttgcttgct gcgtatatga tggtttatta cgtgggaacg tttttaggcc agttactggt
    29821 cagcaaagtc tcaaccgagc tgatgagcgt attgccgtgg gttacaggtt tgacattggc
    29881 agggatctta ccgctgttgt ttacgcgtgt cctgaatcag caggctgaaa accatgattc
    29941 gacgtcaatt acggcaatgc taaaactccg tcaggcgcgg cttggcgtga atggctgcat
    30001 tatctcagga atcgttctgg gatctctata tggcctgatg cctctgtacc tcaatcacaa
    30061 aggggtgagc aatgccagta ttggtttctg gatggcggta ctggtcagtg cgggtatcct
    30121 cggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg ttttgcgtgt
    30181 tcaggtcttt gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg cgatggcccc
    30241 agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga tggcctgggc
    30301 ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac caagccttac tgttgagcta
    30361 taccgtggga agtctgcttg gcccgtcatt tagcgctatg ctaatgcaga atttctccga
    30421 taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga tgctgcttcg
    30481 caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa gcaa
//
LOCUS       DAJTUN010000038        30014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTUN010000038 DAJTUN010000000
VERSION     DAJTUN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.038"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO470_001624"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO470_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6250905.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO470_001625"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO470_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6250906.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO470_001626"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO470_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6250907.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO470_001627"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO470_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6250908.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO470_001628"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO470_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6250909.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO470_001629"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO470_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6250910.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO470_001630"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO470_001630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6250911.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO470_001631"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO470_001631"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6250912.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO470_001632"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO470_001632"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6250913.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO470_001633"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO470_001633"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6250914.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO470_001634"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO470_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6250915.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO470_001635"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO470_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6250916.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO470_001636"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO470_001636"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6250917.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO470_001637"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO470_001637"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6250918.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO470_001638"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO470_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6250919.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO470_001639"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO470_001639"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6250920.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO470_001640"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO470_001640"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6250921.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO470_001641"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO470_001641"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6250922.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO470_001642"
     CDS             21622..22212
                     /locus_tag="OO470_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6250923.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO470_001643"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO470_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6250924.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO470_001644"
     CDS             22684..23379
                     /locus_tag="OO470_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6250925.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO470_001645"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO470_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6250926.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO470_001646"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO470_001646"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6250927.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO470_001647"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO470_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6250928.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO470_001648"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO470_001648"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6250929.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO470_001649"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO470_001649"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6250930.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7030 a         7822 c         8256 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgattcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacttg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatgtcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgtgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gaga
//
LOCUS       DAJTUN010000039        29155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTUN010000039 DAJTUN010000000
VERSION     DAJTUN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.039"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1879)
                     /locus_tag="OO470_001650"
     CDS             complement(<1..1879)
                     /locus_tag="OO470_001650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6250931.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     AL"
     gene            2011..3120
                     /gene="apbC"
                     /locus_tag="OO470_001651"
     CDS             2011..3120
                     /gene="apbC"
                     /locus_tag="OO470_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6250932.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3382..3663
                     /locus_tag="OO470_001652"
     CDS             3382..3663
                     /locus_tag="OO470_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6250933.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            3956..4480
                     /locus_tag="OO470_001653"
     CDS             3956..4480
                     /locus_tag="OO470_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6250934.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            4561..5235
                     /locus_tag="OO470_001654"
     CDS             4561..5235
                     /locus_tag="OO470_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6250935.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5251..7731
                     /locus_tag="OO470_001655"
                     /pseudo
     CDS             5251..7731
                     /locus_tag="OO470_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            7747..8780
                     /locus_tag="OO470_001656"
                     /pseudo
     CDS             7747..8780
                     /locus_tag="OO470_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(8862..9200)
                     /gene="rcnB"
                     /locus_tag="OO470_001657"
     CDS             complement(8862..9200)
                     /gene="rcnB"
                     /locus_tag="OO470_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6250936.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9419..10267)
                     /gene="rcnA"
                     /locus_tag="OO470_001658"
                     /pseudo
     CDS             complement(9419..10267)
                     /gene="rcnA"
                     /locus_tag="OO470_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10389..10661
                     /gene="rcnR"
                     /locus_tag="OO470_001659"
     CDS             10389..10661
                     /gene="rcnR"
                     /locus_tag="OO470_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6250937.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      10753..10849
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10884..11672
                     /gene="thiM"
                     /locus_tag="OO470_001660"
     CDS             10884..11672
                     /gene="thiM"
                     /locus_tag="OO470_001660"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6250938.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            11669..12469
                     /gene="thiD"
                     /locus_tag="OO470_001661"
     CDS             11669..12469
                     /gene="thiD"
                     /locus_tag="OO470_001661"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6250939.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            12534..13355
                     /locus_tag="OO470_001662"
     CDS             12534..13355
                     /locus_tag="OO470_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6250940.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13403..14149
                     /locus_tag="OO470_001663"
     CDS             13403..14149
                     /locus_tag="OO470_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6250941.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14123..15088)
                     /locus_tag="OO470_001664"
     CDS             complement(14123..15088)
                     /locus_tag="OO470_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6250942.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGDDCAPTREELLLAHKNV"
     gene            complement(15085..16088)
                     /locus_tag="OO470_001665"
                     /pseudo
     CDS             complement(15085..16088)
                     /locus_tag="OO470_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16085..17362)
                     /locus_tag="OO470_001666"
     CDS             complement(16085..17362)
                     /locus_tag="OO470_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250943.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17619..18671
                     /gene="fbaB"
                     /locus_tag="OO470_001667"
     CDS             17619..18671
                     /gene="fbaB"
                     /locus_tag="OO470_001667"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6250944.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18979..19833
                     /gene="gatY"
                     /locus_tag="OO470_001668"
     CDS             18979..19833
                     /gene="gatY"
                     /locus_tag="OO470_001668"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6250945.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19862..21124
                     /gene="gatZ"
                     /locus_tag="OO470_001669"
     CDS             19862..21124
                     /gene="gatZ"
                     /locus_tag="OO470_001669"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6250946.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21134..21586
                     /gene="gatA"
                     /locus_tag="OO470_001670"
     CDS             21134..21586
                     /gene="gatA"
                     /locus_tag="OO470_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6250947.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21617..21901
                     /gene="gatB"
                     /locus_tag="OO470_001671"
     CDS             21617..21901
                     /gene="gatB"
                     /locus_tag="OO470_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6250948.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21905..23260
                     /locus_tag="OO470_001672"
     CDS             21905..23260
                     /locus_tag="OO470_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6250949.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23308..24348
                     /gene="gatD"
                     /locus_tag="OO470_001673"
     CDS             23308..24348
                     /gene="gatD"
                     /locus_tag="OO470_001673"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6250950.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24454..25227
                     /locus_tag="OO470_001674"
     CDS             24454..25227
                     /locus_tag="OO470_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6250951.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25309..26208)
                     /gene="yegS"
                     /locus_tag="OO470_001675"
     CDS             complement(25309..26208)
                     /gene="yegS"
                     /locus_tag="OO470_001675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6250952.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            26614..26931
                     /locus_tag="OO470_001676"
     CDS             26614..26931
                     /locus_tag="OO470_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250953.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27273..28634)
                     /gene="yegQ"
                     /locus_tag="OO470_001677"
     CDS             complement(27273..28634)
                     /gene="yegQ"
                     /locus_tag="OO470_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6250954.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28781..29112)
                     /locus_tag="OO470_001678"
                     /pseudo
     CDS             complement(28781..29112)
                     /locus_tag="OO470_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7582 a         6751 c         7378 g         7444 t
ORIGIN      
        1 tcagtgcctg cggatccggg taagcagaac gaataccgga gaagacattg cgtttttcac
       61 cgccgagatc cagcgtcagg cgcagcagtt tgtcagaacc ttcaacaaac tctgcgtttt
      121 cgatcagcgc cacgcgcagg tcaactttgg cgaagtcgtc aaaggtgatg gtttcctgaa
      181 tcggatcatc tgccagtggg ccagtaactg gcgcggtagt ggctttcact tcttctttag
      241 aggcttccac cagagcttca acctgtttca tatcgatgcg gttatacagc gccttgaacg
      301 gattcacttt gtggcccagc agcggttgct ggataccatc ccaggtcagt tccgtattga
      361 ggaaggcttc tgcacgctcg gtcagtttcg gcagtaccgg cttcaggtaa gtcatcagca
      421 cgcggaacag gttgatgccc attgagcaaa tcgcctgcag gtcagcatcg cgaccttcct
      481 gttttgcgac tacccacgga gcctgctcgt cgacatagcg gttagccagg tcagccagtg
      541 ccatgatttc gcgcactgct ttaccaaatt cacggctttc ccacgcttca ccaatcactt
      601 cagcggcatc ggtaaaggtt ttgtacagct cagggtcagc cagctcgctt gccagcacac
      661 cgtcaaaacg cttgttgata aagcccgcat tacgggaggc caggttaacc actttgttca
      721 cgatatcggc attcacacgc tgaacgaaat cttccaggtt gagatcgata tcatcaatgc
      781 gcgaagagag tttcgcagtg tagtagtaac gcaggctgtc agcgtcaaaa tgattcagcc
      841 aggtgctggc tttaataaag gtgccgcgag acttggacat cttcgcgccg ttcaccgtca
      901 catagccatg aacaaacagg ttggtcggct tgcggaagtt gctgccttcc agcatggcag
      961 gccagaacag gctgtggaag taaacgatat ctttaccgat gaagtggtac agctcggcgg
     1021 tggagtcttt cttccagtat tcatcgaagc ttacgctgtc gccgcgcttg tcgcacagat
     1081 tcttgaaaga acccatgtag ccaatcggtg cgtccagcca gacgtagaaa tatttgcccg
     1141 gtgcgttcgg aatttcaaaa ccaaagtatg gcgcgtcgcg ggagatatcc cactgttgca
     1201 ggccggattc aaaccactcc tgcattttgt ttgccacctg ctcctgcaac gcaccgctgc
     1261 gggtccatgc ctgcagcatt tcgctgaaag agggcagatc aaagaagaag tgttcagaat
     1321 cacgcatcac tggtgtagca ccagaaacca ccgatttcgg ctcgatcaat tcagtcgggc
     1381 tgtaggtcgc gccgcagact tcgcagttat cgccgtattg atccggggat ttacatttcg
     1441 ggcaggtgcc tttcacaaaa cggtccggca ggaacatgcc tttttccgga tcgtacagct
     1501 gagagatggt gcggttttta ataaaaccgt tttctttcag gcgagagtag ataagttctg
     1561 acaactggcg gttctcttcg ctgtgcgtcg agtgatagtt gtcatagctg atgttaaagc
     1621 ctgcgaaatc agtctgatgc tcctgactca tttcgccaat catctgctcc ggggtgatac
     1681 cgagctgctg agctttcagc atgatcggtg taccgtgggc atcgtcggcg cagatgaagt
     1741 tgacctcgtg gccgcgcatt cgctggtaac ggacccagac atcagcctgg atgtgctcca
     1801 gcatatggcc gaggtggatt gagccgttag cgtacggcag tgcgcacgtc accagaattt
     1861 tcttcgcgac ttgagtcata gtaggcatta cttcttaata gtgaaaaggg gttagatagt
     1921 accaaatggg aaaatgttaa gtaagtactt ctctatattc ttcaggtaaa ctatcagctg
     1981 atggtttatc tatacaaaaa aggagtcggg atgaacgaac aatcccaggc caaatcacca
     2041 gaagccttgc gcgcaatggt cgccgggaca ctggccaatt ttcagcaccc aaccctgaag
     2101 cataacctca ccacgctgaa agcgttgcac catgtcgcct ggatggacga cacgttgcat
     2161 gtggaactgg ttatgccgtt tgtctggcat agcgcgttcg aagagttaaa agagcaatgt
     2221 agcgccgaac tgctgcgcat caccggggcg aaagctatcg actggaaact gtcgcataac
     2281 atagccactc tgaaacgcgt gaagaatcag ccgggaatta acggtgtgaa gaacattatc
     2341 gcggtgagtt ccggtaaagg cggcgtgggt aaatcgtcca cggcggtaaa cctggcgctg
     2401 gcactggcgg cggaaggggc gaaagtcggc attctggacg ccgatatcta cggcccatca
     2461 attcccacca tgctgggcgc agaaaatcag cgtccaactt cgccggacgg tacccacatg
     2521 gcaccgatta tgtctcatgg cctggcaacc aattcaattg gttatctggt cactgacgac
     2581 aatgcgatgg tgtggcgtgg accgatggcg agcaaggcgt taatgcagat gttgcaggaa
     2641 accttgtggc cggatctcga ctatctggtg cttgatatgc cgccaggcac cggtgacatt
     2701 caattgacgc tggcgcaaaa cattccagta actggtgcag tcgtggtcac caccccgcag
     2761 gacatcgcgt tgatcgatgc gaaaaaaggc attgtgatgt tcgagaaagt cgaggttccg
     2821 gtgctcggta ttgtcgagaa catgagtgtg catatttgca gtaactgtgg tcaccatgaa
     2881 ccgattttcg gtaccggtgg tgcagagaaa ctggctgaaa aataccatac acagctgctg
     2941 gggcagatgc cgttacacat ctccttacgt gaggatctcg ataaaggcac gccaacggtt
     3001 atcagccgtc cggagagcga atttaccgct atctaccgcc agctggctga ccgcgttgca
     3061 gctcagctct actggcaggg tgaagtcatt cctggcgaga tttccttccg cgcggtctaa
     3121 cgcctattaa ccattctggt tattcttcat acaccgcaga gatgttatat tgatgcggtg
     3181 tatttgtttt tgccgcatta atgaacactt atttaatact gcgaatattc tgcggacctt
     3241 cgtccgaaac tctacaaagt cgagtgatcg tacaggcaat gcttcattat catccttcgt
     3301 gctgtgtagt ggatgtacag ctatagtttg ttatgcactc attctatcca ggcataacct
     3361 ttactacagg gataacgttc aatgaataag tattggctgt caggaattat ttttcttgcc
     3421 tatggattgg cttctcccgc agtctcttca gagactgcca cattaacgat taatggcagg
     3481 atcagtcccc caacatgcag tatggctatg gttaatagtc aaccacaaca gcattgtggt
     3541 cagttaacgt ataatgttga cacccgtcat caggtttctt cgcccgtcaa aggtgttacg
     3601 acagaagtgg ttgttgcagg tagcgatagc aaacgtcgaa tcgtgttaaa tcgctacgat
     3661 taatttggca acctgatttt aattgcattt agaaagacga aatcatttat ataaatgatt
     3721 tcgtcttttt tattgatatc aacaatacca tttacataaa tctacattaa ttaatagatg
     3781 tgataaatat ttaatattct taagtatttg ttgtatttat attgcattgg ttaatttgtt
     3841 ttaaatcaat aaaacaatat gtttacattg cggcagcttt tttgatgtga gaaatttgtt
     3901 tccaagagat acagactctt aacaaccaaa aataaatttt attaaggaac atattatgaa
     3961 acgttcaatt attgctgccg ctgtcttttc ttcttttttt atgagcgctg gagtatttgc
     4021 tgcagacgtt gataccggaa cattaaccat taaaggaaat attgcagaat ctccgtgtaa
     4081 attcgaagcg ggtggtgatt cagtaagtat taatatgccg actgtaccaa ccactgtctt
     4141 tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc ggtgtaacca gcagcatgtt
     4201 aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc tcgttgatca caaacgacaa
     4261 aataaccggt aacgataagg cgatagccag tagcaacgat accgtaggtg ataacagcga
     4321 tgtcctagat gtttctgcac cttttaacat tgagagttat aaaacagcgg aaggtcaata
     4381 tgctattccg tttaaagcaa aatacctgaa actgacagat aactcagtgc aatcaggtga
     4441 tgtgttatct tctctggtta tgcgtgtggc gcaggattaa taaataaaat tgcaagtttc
     4501 tccaggtaat aaacatggct gctatcccca tggcggcttt ttaatttaag aggcattaag
     4561 atgaaaggat tattatcttt actcattttt tctatggtcc ttcctgcaca tgccggaatt
     4621 gttatctacg ggacgcgcat tatttacccg gcagaaaata aagaagttat ggtgcagttg
     4681 atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga ttgatgatgg cgatacgtcg
     4741 ttaccaccag aaaaaattca ggttcctttc atgttaacgc caccagtggc aaaaataggg
     4801 gcaaattccg ggcagcaagt aaaaatcaaa attatgccga ataaactgcc cactaataaa
     4861 gaaagcattt tttatctgaa tgttctgtat attccaccaa atagtccaga gcaagaaggc
     4921 aagaatgcac tgaagtttgc gatgcaaaac agaattaagt tgttttaccg gccagcgggt
     4981 attgctccgg taaataaagc gacctttaaa aagttactgg taaatcgcag tggcaatgga
     5041 ttggtgataa aaaatgactc agctaattgg gtgacgattt cggatgtcaa agctaataat
     5101 gttaaagtca attatgaaac tattatgatt gcccccttag aaagtcagag tgttaatatc
     5161 aaaagtaata atgcaaataa ctggtatctg accattatca atgaccacgg caactatatt
     5221 agtgacaaaa tttaattgca ggagctgcct atgttgagaa tgaccccact tgcatcagca
     5281 atcgtagcgt tattgctcgg cattgaagct tatgcagctg aagaaacctt tgatacccat
     5341 tttatgatag gtggaatgaa agaccagcag gttgcaaata ttcgtcttga tgataatcaa
     5401 cccttaccgg ggcagtatga catcgatatt tatgtcaata agcaatggcg cgggaaatat
     5461 gagattattg ttaaagacaa cccgcaagaa acatgtttat caagagaagt tatcaagcgg
     5521 ttaggcatta atagcgataa cttcgccagc ggtaagcaat gtttaacatt tgagcaactt
     5581 gttcagggtg ggagctatag ctgggatatc ggggtttttc gtctcgattt cagtgtcccg
     5641 caggcttggg tggaagaact ggaaagtggc tatgttccac cggaaaactg ggagcggggt
     5701 attaatgcgt tttatacttc ttattatgtg agtcagtatt acagcgacta taaagcgtcg
     5761 ggtaacaaca agagtacata tgtacgtttt aacagcgggt taaatttact ggagtggcaa
     5821 ctgcattctg atgccagttt cagtaaaaca aataacaatc caggggtgtg gaaaagcaat
     5881 accctgtatc tggaacgtgg atttgcccaa tttctcggca cgcttcgcgt gggtgatatg
     5941 tacacatcaa gcgatatttt tgattctgtt cgcttcagcg gtgtgcggtt gtttcgtgat
     6001 atgcagatgt tgcctaactc gaaacaaaat tttacgccac gggtgcaggg gattgctcag
     6061 agtaacgcgc tggtaactat tgaacagaat ggttttgtgg tttatcagaa agaggttcct
     6121 cctggcccgt ttgcgattac agatttgcag ttggccggtg gtggagcaga tcttgatgtt
     6181 agcgtgaaag aggcggacgg ctcggtaacc acctatctgg tgccttatgc agcggtgcca
     6241 aatatgctgc aacccggcgt gtcgaaatat gattttgcgg cgggccgtag ccatattgaa
     6301 ggggcgagca agcaaagtga ttttgtccag gcaggttatc agtatggttt taataactta
     6361 ttgacgctgt atggcggcac gatggttgct aataattact atgcgtttac cctctgaacg
     6421 ggttggaaca cgcgcattgg cgcaatttcc gtcgatgcca cgaagtcgca tagtaaacaa
     6481 gacaacggcg atgtgtttga cgggcaaagt tatcaaattg cctacaacaa atttgtgagc
     6541 caaacgtcga cgcgttttgg tctggcggcc tggcgttatt cgtcgcgtga ttaccggaca
     6601 tttaacgatc acgtttgggc aaacaataaa gataattatc gccgtgatga aaacgatatc
     6661 tatgacattg ccgattatta ccagaacgat tttggccgta aaaatagctt ttctgccaat
     6721 atgagccagt cattgccaga aggctggggt tctgtgtcct taagtacgtt atggcgagat
     6781 tactgggggc gtagcggcag cagtaaggat tatcagttga gttattccaa taactggcgg
     6841 cggataagct ataccctcgc ggcaagccag gcgtatggcg agaatcatca tgaagagaaa
     6901 cgttttaata tttttatatc aattccctgt gattggggtg atgacgttac gacgcctcgt
     6961 cggcaaatat atatgtctaa ctcaacgacg tttgatgatc agggttttgc ctcaaataat
     7021 acgggattat caggaacagt agggagtcgg gatcagttca attatggtgt caacctgagt
     7081 catcaacatc agggaaatga aacgacagct ggggcgaatt tgacctggaa cgcgccggtt
     7141 gcgacagtga atggcagtta tagtcagtcg agtacttatc gacagactgg agccagtgtt
     7201 tcagggggca ttgtcgcctg gtcgggtggc gttaatctgg cgaaccgtct ttccgaaacg
     7261 tttgctgtga tgaatgcgcc aggaattaaa gatgcttatg tcaatgggca aaaatatcgc
     7321 acaacaaacc gtaatggagt ggtggtatac gacggaatga caccttatcg ggaaaatcac
     7381 ctgatgctgg atgtgtcgca aagcgatagc gaagcagaat tacgtggcaa ccggaaaatt
     7441 gccgcccctt atcgcggcgc ggttgtactg gttaattttg ataccgatca gcgcaagccc
     7501 tggtttataa aagcgttaag agcggatggt caaccattaa cgtttggtta tgaagtcaat
     7561 gatatccatg gtcataatat tggcgttgtc ggccagggaa gccagttatt tattcgcacc
     7621 aatgaaatac cgccatcggt taatgtagca attgataagc aacaaggact ttcatgcaca
     7681 atcaccttcg gtaaagagat tgatgaaagt agaaattata tttgccagta atagtcaggt
     7741 ggttctatgg aaattcgcat aatgctattt atattaatga tgatggttat gcctgtgagc
     7801 tatgcggcat gttatagtga gttatctgtt cagcacaatt tggttgttca ggggaatttt
     7861 gcacttactc aaacacaaat agcgacatat gagcataatt ttaatgattc gtcatgcgtg
     7921 agtacaaata ctatcacccc tatgagccca tcggatatta ttgttggact ttataacgat
     7981 accattaaat taaatttaca ttttgaatgg accaataaaa acaacatcac gttgtcaaat
     8041 aatcagagca gtttcaccag tggttattcg gtaacggtga cacctgcggc cagtaatgca
     8101 aaagtgaata tttctgcggg gggcggtggt tcagtgatga ttaatggtgt tgcgacatta
     8161 tccagtgctt catcatcgac acgcgggagt gccgcagtac aatttctact gtgtttatta
     8221 ggtggcaagt catggggtgc atgtgtaaat agctacagaa atgcattggc acaaaatgca
     8281 ggtgtctatt ccttcaatct gacattgtca tacaacccga taaccacaac ctgcaaacag
     8341 gacgatttat taattacttt agacagtatt cccgtttcac aattaccagc cacaggtaac
     8401 aaagcaacaa taaatagtaa aaaaagggat attattctgc gttgtaaaaa tttattaggt
     8461 caacaaaatc aaacatcacg gaaaatgcag gtgtatttat caagttctga cttgttaacc
     8521 aacagcaaca caatactgaa aggtgcggaa gataatgcgt aggatttatt cttgaaagta
     8581 atggttcgcc agtcacactt ttaaatatca ctaacagcag taaaggatac acaaatttaa
     8641 aagaaattgc ggcaaagtca aaacttacag atacaacggt ttcaattccg ataacagcca
     8701 gttactacgt ctacgataca aacaaagtta aatctggcgc actggaggca accgcattaa
     8761 tcaacgtgaa atacgactaa taacaagcaa aacgagcaag tggctaataa taaaaaataa
     8821 caaggtgtct gcatttcccc ctcatgatga gggggctttt tttagcgatg ataaaaaatc
     8881 tcaccgtcgt aggctttaat gattttaccg tcggtgtcgc tgatcaacac gtacgcgcca
     8941 cccatatagg tccagtgcgt cccggcatca ggcgcgggca ggttacgtaa ctgccactgc
     9001 ttaatgttgt actcatcggt gcggtacatc tctgggacgg tatcacctgg tttgaaatgc
     9061 ttaaagtcag caatgaaact acttaactca tacttagaaa ttcccgaggt attggtcgaa
     9121 cccgcggtgg ctggtgcggc ccaggcggca ctggtaacca gcaaaagcac acccagcaac
     9181 atcttatttt taatggtcat aaactctcca ctttggggat ctgtaatatc agtcgcatat
     9241 ctttcctgtc atacaatata actgacaagc gatattttgt tagtgatccc atgatttcgt
     9301 aaaaagtgta aatgaaagga tgccctaaaa gcatccttag cgagcagaga gtcagcggaa
     9361 atagctgatt taagaatcat cctgggctga agataacatg caagcctaaa ggattgagtt
     9421 atcgcattat gcccatgaag ccatgtacgc ccatatacac accgactaag ccaatcaaca
     9481 gactggaaaa atagggggcg cgtttagcga gagtgttaaa tccgctccag cgttttgcga
     9541 cctgctgaac gctgattgct gcgccaacgc ctacggtgac aagcgttaac gccaggccaa
     9601 tgctgaaact gacgaccagt gttgcgccca gtgtcagggc tttcaactga atgcatatca
     9661 acagcacggt aattgctgcc gggcagggga taaagccacc ggttaagcca aataacaaaa
     9721 tttgccagtt gatgacctct ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc
     9781 gtgcatgggc atcctgatac tcgccatatt cgtgatggtg atgatgtcct tggtcgtggt
     9841 gatgttcgtg gtcttgatga tgttcgtgat cgtgatgatg gtgatgatgc tcataatcat
     9901 gcccgtgcat attctccagc cagttgcgtt cgccgcgcta ggtacgccag aacatccaga
     9961 acgcggtgcc aataatgatc actgcggaaa tcagctggag ccacggttct gctgattgag
    10021 cagtaaagcg cttactgatc accatcccgc caaaggcaat caaccagacc accgcggtat
    10081 gcgaaatggt tgctgccagt ccgagcatca ccgcctgttt aatggtgcct ttgatggcga
    10141 tgataaacgc cgccatcatc gtttttgagt gccctggttc caggccatga agcgcaccaa
    10201 gtaagatggc gctggggatg aagaaccagg cgtttccttg ctgaagaaga gttgtaaatt
    10261 cggtcatgat aataattctt agtattaatt cggcaatctg attctactcc cccccagtac
    10321 ctgatactac ccccccagta gattaatagt gctatgattt ttcatattct tgttaaccag
    10381 gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt gccagtaaga
    10441 ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga gccgcacgaa tgcgctgcag
    10501 ttttacaaca gattgctgct atccgtggag cggtaaacgg tctgatgcgg gaagtgatta
    10561 aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt gaagaagatc
    10621 tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac gcgattgtaa
    10681 aactgccgtt tttcctcgtt tacaacgggg gcgctggaca ttaccatcct cctctgcgat
    10741 ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag aaatacccgt
    10801 atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt cattgcttct
    10861 tcacgttatg gcaggagcaa actatgcaag tcgacctgct gagttcagcg caatctgcgc
    10921 acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc aatgatgtgg
    10981 tgcaaacctt taccgccaat accttgctgg cgctcggtgc atcgccagcg atggttatcg
    11041 aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt aacgttggca
    11101 cactgacgca gccacgcgca caggcgatgc gtgctgccgt tgagcaagca aaaagctctc
    11161 aaacacgctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc cgccattttt
    11221 gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg gaaatcatgg
    11281 cattagctgg cattgctaat ggcggacggg gagtggatac cactgacgcc gcagctaacg
    11341 cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg gtcactggcg
    11401 aggtggatta tgttaccgat ggacatcgta tcattggtat tcacggtggc gatccgttaa
    11461 tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc tgctgtgcgt
    11521 taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa caagccggag
    11581 aacgcgcagt cgccagaagc gaggggccag gcagttttgt tccacatttc cttgatgcgc
    11641 tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga cgattgccgg
    11701 tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct cggcacttgg
    11761 cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg gcgtacagtc
    11821 ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt tcagcgatgt
    11881 gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg aagcggtggc
    11941 agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta tgctggcaaa
    12001 aagcggcgac ccgctgcttt caccttcggc ggttgctacg ctgcgcagtc gattattgcc
    12061 acaggtttca ttaataacgc caaacttgcc cgaagctgcc gccttgctcg acgcgccaca
    12121 cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga tgggctgtgg
    12181 cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact ggctgtttac
    12241 ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca cccacggcac
    12301 tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa actgggctga
    12361 caccgtacag gaggcaaaaa tctggctttc atcggcgtta gcccaggccg acacgctgga
    12421 agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag caccgtacta
    12481 tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa ccaatgcagc
    12541 taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg ctaatttcca
    12601 tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca gttcagatta
    12661 cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt cgcattcctg
    12721 ccagttacac cattcacgga ttagatgttt cgcgctggca ggagcgtatc gactggcagc
    12781 gggtggcaaa aatgcgcgac aacgatatcc gcttacagtt tgcttttatt aaggctacgg
    12841 aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaattaagc cgcgaaaatg
    12901 gcctgctgcg cggggcgtat cattatttct ccccgtcggt atccgcttca gttcaggcgc
    12961 gattatttct gcaaacggtg gatttctcac aaggcgattt ccctgccgtg ctggacgtag
    13021 acgaacgggg aaaattatcg gcaaaagaat tacgcaagcg ggtaagtcag tggctaaaaa
    13081 tggtcgaaaa acgcacggga aaaaagccga ttatttactc aggagccgtt ttttatcaca
    13141 ccaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactat tatcaacgtc
    13201 gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt ggacaggtag
    13261 atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggggatg agttgcaggc
    13321 attcgttgat gggattaaag aaacgcctta aataagaagc agtgatatac tcccaccaga
    13381 ctccctggca ggaaaaagac aaatggaaca agcgcatacc cagctgattg cccaactgaa
    13441 cgagcgtatt ttagcggcgg ataacacgcc gctttatatt aagtttgccg aaacggtaaa
    13501 aaatgccgtg cgcagcgggg tgctggagca tggcaatatt ttgcccggtg agcgtgattt
    13561 aagtcagtta accggcgttt cgcgcattac ggtgcgcaag gcgatgcagg cgctggaaga
    13621 agagggcgtg gtgacgcgtt cgcgcggtta cggcacgcag atcaacaaca tcttcgaata
    13681 ctcgttgaaa gaagcgagag gtttttctca gcaggtggta ttgtgcggga aaaagcccga
    13741 tacgctatgg gttaataagc gtgtcgtgaa atgtcccgaa gaagttgcgc agcagctggc
    13801 ggtcgaagcg ggaagtgatg tctttttgct taagcgtatt cgctatgtcg atgaagaagc
    13861 ggtatcgatt gaggaatcgt gggttccggc gcatttaatt catgatgttg atgccatcgg
    13921 aatttctctt tatgactatt tccgcagcca gcacatttac ccacaacgta cgcgttcccg
    13981 cgttagcgcc cggatgccgg atgccgagtt tcagtcacat attcagttag atagcaaaat
    14041 accggtgctg gtgatcaagc aagtggcgct tgaccaacag caacggccta ttgagtacag
    14101 cattagccac tgtcgcagcg atctatacgt ttttgtgtgc gaggagtagt tcctcgcgcg
    14161 ttggcgcaca atcatcgccc cgatgcccga caacccacga cgccactgca ttgcccagta
    14221 atacggcatc cgccagtggc agaccagagg ccagcccggc aagtacgcca cctgcatgac
    14281 tgtcgcccgc cccaatggtg tctacaactt gcgttggaaa tgccggaatg cagccagaag
    14341 cgtcgttgct gaaataccat gcgccttctt tatcgagacg aacgatcaac ggcgcggcaa
    14401 atttctcctg ccattgcttg ccaagtgttg ttatctctgc ggataaagca aaacgttcgg
    14461 cggcaatctc agcctcttga cgattgagtg acactaaagg tcgacaggcc atgatccgcg
    14521 ccagtaatgc gtccgggata tcgccaatac gtgggcaaaa atcgataaac ggcgtcacgt
    14581 cttgcagctt ttccagccat tccactaaca attcgccgca gggcgaggcc agttgataac
    14641 cggaaaaata gagcaggttg ccaggcgcaa cggttaatcg tgccaaccat tggcgattcc
    14701 attgattttc cacaccgctg aatgacataa aagtgcgttc gccatccggc tcaaccagtg
    14761 ccagacacca gccgttatcg ctttcggcgt tatcgatcag gctgattaac ccctctttcg
    14821 ccatccggtt gcgaataatc tccgcccaca cgccctgacc gagcggcaag gcattacccg
    14881 cttcgatgcc gaggcgcttt aacgccacgg caatattcag tgcgcagccg ccaacgttaa
    14941 cgctctgctg tttcagttcg atatcgcacc cacgccaggg gagggcataa gcgtcggcga
    15001 tcacgtcaat gaccgccgcg ccgacgacca tcaccggctg acgcgtggtt aattcaggca
    15061 gcagtgtgtg taatcgagcg ccgctcatac cgcctcccgt tgttgacgat atttcgccag
    15121 cgctgtggca tagcggttga aatcaagctg atttaccgca tccagctccg cctttaacgc
    15181 aggatcgaca gcgttaatgc catgcaacgc gccgcaaatc gccgtcgcca tagcacctat
    15241 ggtgtctgtg tcgccgccaa gattggcgca caggacggcg cagcggtttg ggtcggtttg
    15301 tgccagttca actagcgcaa tggcgcacgg aacggactca atagtgctgg tgcctgcgcc
    15361 aacgacctgg taaagctgtt cgctggcgga ttcggtgccg tcggcattac gcacaatttt
    15421 cagcgcaatc tccagacgtg ccgccagtga ggcgctgaac gtggtggtgc gtttttgctg
    15481 tgcatgtcgc gcaattgaag gcaatgaatc aacaatcgct gaccagcgtt ctccgtcaat
    15541 ggcacgagaa atcgcccatg cgatgactac cgcgcctgca accgccagat cggatttatg
    15601 ggtcgggctg gacgccagcg ccacatcatc aataaaggaa tcaacatcac gcgctggaag
    15661 caaacaacct aatggcgaga cgcgcatcgc tgcgccgttg gttacgccgt tgttttccag
    15721 ttcagcaacg ggtttaccgt cgcgaatggc gttaagcgca atcttcgagg tcggacctag
    15781 tacgtttttg ttaaaggcgt cgaaacgcaa cgcccagtcg agaatattac gcccaatcag
    15841 attcggatcg atcttgcctt cacattccag taacgcatcc gccagacaca ggccatcgag
    15901 gtatcgtcgg tgaattcggc gcggttaaaa taacaggccg cgttattctc ctttggtcca
    15961 ggcagaaaac ggtcaatcca gccaaagtgt gctttaacgc ggctgcgtgg ccaaagctcg
    16021 gagggcatcc ccatcgcatc ccctaacgcc tgcccataaa gagcaccgag aatacgttct
    16081 gttttcattt aacttcccct tgtgtcaacg caatatctcg atcatcgacc ttgatagccg
    16141 taatttcgtt gtcggattcg cggaaaaaaa tcatgaacag cacggcgata atggcaatca
    16201 tcaccgcgcc gaaagtccac atcccggacc agttgaaagt cagtccgttt accggttcct
    16261 gataagcgaa catcttttcc atcatcacac cgccaagacg atagccgagc aaactgccga
    16321 agccctggca gcagagcgtg atcagcccct gcgcagcggt acgcatatgc acgggggctt
    16381 ttttatcgac atagatgtaa gcggtaacgt agtaaaaatc gtaacttacg ccgtgaagca
    16441 aaataccgag gaacagtaac gcgtaggtga aatattcatc cgcactaccg taaataaaga
    16501 agccatagca gatcgcagcg gtgaccagac caagcaataa tacctttttg ataccaaagc
    16561 gtttagtgaa aaacggcaat gccagcataa agaagatttc agagaactgg ccgagcgtca
    16621 tccagccggt ggcgtttttc atgccaactt cggtcagata accgttggca aagatgtaat
    16681 agaacgccag tggcatcgca aacaggaatg aacagaagaa aaagacgagg aagtttttat
    16741 cgcgcagcag gatcagcgca tccaggccga gcatgacttt aatgtccatt ttgccggtgc
    16801 tttttggcgg cgtgtcgggc aggaaaaacg caaacacacc gagcagagca gaacttccgg
    16861 cggtaatcag cagcgggatg ttagtcggtg agatatcggc ataccccagc atttgcggca
    16921 agaaaccaca tgccagaccg gaggcaatcc agccgatagt gcccatcaca cgaatgcgtg
    16981 ggaaatcacg ctccacatcc ggcacgttgg caaaagcgat gctgttagtc agcgcaatgg
    17041 tcggcatata ggttagcgag taggccagca gtaacggaaa gaacccggca aaagtggtct
    17101 gttgcgcagc gaaatacatc agcacagcac cagcgaacat caataccgcc agcactttct
    17161 gcgccgagaa aaagcggtca gtgatggagc caaccagaat tggcgacagg atcgccgcaa
    17221 tggctgtgca ggcatacgac cagccaattt ctccggcact aaaaccgctt ttacttaacc
    17281 acaaccacaa tggcacaaac cacgcgcccc agataaacca ttcaacaaac atcatgaacg
    17341 acagctttgc tgttgttttc atcttttaat tccttcatga caggtaatgt aaggtacgcg
    17401 atgacaatac cacataataa tgccttttga atacctttgc ggtgaaaact tgaccgcgct
    17461 aacatttttt ctgatgaatc gagccaacag aaaacgctga aaaaacatcc aaaagatgga
    17521 aaaactcgcc cgtaacgact aggattatga ttgcccgcaa aaggcgggcc aggacaatcc
    17581 atagccgata tccaatcgga atttacggga gcatagtaat gacagatatt gcgcagttgc
    17641 ttggcaaaga cgccgacaac cttttacagc accgttgtat gactattcct tctgaccagc
    17701 tttatctccc cggacatgac tacgtagacc gcgtgatgat tgacaataat cgcccgccag
    17761 cggtgttacg taatatgcag acgttgtaca acaccgggcg tctggctggc acaggatatc
    17821 tttctattct gccggttgac cagggcgttg agcactctgc cggagcttca tttgctgcta
    17881 acccgctcta ctttgacccg aaaaacattg ttgaactggc gatcgaagcg ggctgtaact
    17941 gtgtggcatc aacttacggc gtgttggtgt cggtatcgcg gcgctatgcg catcgcattc
    18001 catttctcgt caaacttaat cacaacgaga cgctaagtta cccgaatacc tacgatcaaa
    18061 cgctgtatgc cagcgtggag caggccttca acatgggcgc ggtggcggtt ggtgcgacta
    18121 tctattttgg ctcggaagag tcacgtcgcc agattgaaga aatttctgcg gcctttgaac
    18181 gcgcgcacga gctgggcatg gtgacagtgc tgtgggccta tttgcgaaac tccgccttta
    18241 agaaagatgg cgttgattac catgtttccg ccgacctgac tggtcaggcg aaccatctgg
    18301 cggcaaccat aggtgcagat atcgtcaaac aaaaaatggc ggaaaataac ggcggctata
    18361 aagcgattaa ttacggttat accgacgatc gcgtgtacag caagttaacc agcgaaaacc
    18421 cgattgatct ggtgcgttat cagttagcta actgctatat gggccgggct gggttgataa
    18481 actccggcgg tgctgcaggc ggtgaaactg acctcagcga tgcagtgcgt actgcggtta
    18541 tcaacaaacg cgcaggcgga atggggctga ttcttggacg taaggcgttc aagaaatcga
    18601 tggctgacgg cgtgaaactg attaatgccg tgcaggacgt ttatctcgat agcaaaatta
    18661 ctattgcctg atacgcctca tacgaacagc accaccccgt aggacggata agacgtttac
    18721 actgcatccg gcaacctatg cctgatgcga cgcggaagcg tcttatcatg cctacatagc
    18781 actgccacgt atgtttacac cgcatccggc ataaaaacac gcgcactttg ctacggcttc
    18841 cctatcggga ggccgttttt ttgcctttca ctcctcgaac aattttcata ttgttgtttt
    18901 tgtgatcatt atctcgacat ttaaaagcaa ataatttcat tatattttga aatcgaaagc
    18961 aaacgacaag atatgaaaat gtacgtggta tcgacaaagc agatgctgaa caacgcacag
    19021 cgcggcggtt atgcggttcc ggcattcaat attcacaatc tcgaaacgat gcaagtggtg
    19081 gtggaaaccg ctgccaacct gcatgcgccg gtcatcatcg ccggaacgcc tggcacattt
    19141 actcatgctg gtacagaaaa tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac
    19201 catccactgg caattcatct cgaccatcac acgaaatttg acgatatcgc tcagaaggtt
    19261 cgttctggcg tgcgctcagt catgattgac gcctcgcatt tgccttttgc gcaaaatatt
    19321 tcacgggtca aagaggtggt ggatttttgc catcgctttg atgtcagcgt cgaggcggag
    19381 ctggggcaac ttggcggcca ggaagatgat gtgcaagtca atgaagtcga tgcgttgtac
    19441 accaaccccg cacaggcgcg tgaatttgcc gaggcaaccg ggattgattc cctggcggtc
    19501 gccatcggca cggctcacgg tatgtatgcc agcgcaccgg tgcttgattt ttctcgactg
    19561 gagaacattc gccagtgggt gaacttaccg ctggtgctgc atggcgcgtc aggattatcg
    19621 actaaggata ttcagcaaac catcaaactg gggatatgca aaatcaacgt tgcaacggag
    19681 ctgaaaaatg ccttcttgca gtcgttaaaa aattacctga ccgagcaccc cgaagcgacc
    19741 gatccccggg attatttgca gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg
    19801 attgccgatt gtggctgcga gggcagggca taacgcacct gccatttaac aaggaaaaaa
    19861 catgaaaacg ttaattgccc ggcataaagc tggtgaacat atcggcatat gttcagtctg
    19921 ttctgcccat ccgttggtta tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa
    19981 agtgctgatt gaagcaacgt caaaccaggt caatcaattt ggcggttata ccggaatgac
    20041 accggcagac tttcgcgaat ttgtttttac gattgccgat aaagttgggt ttgcacgcga
    20101 acgcattatt ctcggcggcg atcatctggg accaaactgc tggcagcaag aaaatgcgga
    20161 tgcggcgatg gaaaaatccg tcgagctggt aaaagcatat gttcgtgctg gcttcagtaa
    20221 aattcatctt gatgcgtcaa tgtcctgcgc gggggatacc ataccgttag caccagaaac
    20281 ggttgcggaa cgagctgctg tgctttgctt tgctgcggaa agtgtggcga cagattgcca
    20341 gcgtgagcaa ctgagctatg tcattggcac tgaagttccg gttccgggcg gtgaggccag
    20401 cgccattcag tcagtacaca tcacccatgt tgaagatgcc gccaatactt tacgtacgca
    20461 tcaaaaggcc tttattgccc gtgggctgac agaggcgtta acacgtgtga ttgccatcgt
    20521 ggtgcagccg ggtgtggaat ttgatcacag caatattatc cattatcagc cgcaggaagc
    20581 gcaggcgctg gcgcaatgga tagaaaacac ccgaatggtt tatgaagcac attctaccga
    20641 ttaccagacc cggacggctt attgggaatt agtccgcgat cactttgcaa tattgaaagt
    20701 cggtcccgca ttaacctttg ctttacgcga ggcgatattt gcactggcac aaattgagca
    20761 ggaacttatc gcccccgaaa atcgcagcgg ttgcctggcg gtaattgaag aagtgatgct
    20821 cgacgaaccg caatactgga aaaaatatta tcgcacgggt tttaacgatt cattactgga
    20881 tattcgttac agcctgtcgg atcgtattcg ttattactgg ccgcatagtc ggattaaaaa
    20941 tagcgttgaa acgatgatgg tgaatctgca aggcgtggac atcccactgg gcatgattag
    21001 tcagtatctt cccaaacaat ttgaacgcat tcagtccggg gaattatcag caatgccgca
    21061 tcagcttatt atgaataaaa tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga
    21121 ataaggacgg tatatgacta acctgtttgt tcgtagcgga atttcttttg tcgatcgtag
    21181 cgaagtttta acctatatcg gtaatgagat gctcgcgaaa ggtgtggttc atgatacctg
    21241 gccacaggca ttaattgcca gagaagcaga attccctacc gggataatgc ttgagcagca
    21301 cgccattgca ataccgcatt gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt
    21361 aaggccaaca aataaagttc attttcagca agcggatgat gataacgacg tggcggtatc
    21421 gttggttatt gcgttgattg tggaaaatcc gcagcagcaa ttgaaacttt tacgctgttt
    21481 atttggcaag ttacaacagc ccgatatcgt cgagacacta attactcttc ctgaaactca
    21541 gttaaaggaa tacttcacaa agtatgtttt agattcagac gaataaatcc ctctgtaaca
    21601 ataaaaagta ctatttatga aacgcaagat tattgtcgct tgcggaggcg cggttgcgac
    21661 ctctacgatg gcggcggaag aaattaaaga gttgtgtcag agtcataata ttcctgttga
    21721 attaatccag tgtcgggtta atgaaataga aacctatatg gatggtgtgc atttgatatg
    21781 taccactgcc agggtggatc gtagttttgg cgatattccg ttagttcacg gcatgccttt
    21841 tgtttctggt gtcggtatcg aagcattaca aaataaaatt ctgactatct tacaggggtg
    21901 acctatgttt tcagaagtca tgcgttatat tctcgacctc ggccctacag tgatgctgcc
    21961 ggttgtcatc attatttttt ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc
    22021 gggtctgcat atcgggattg gctttgttgg cattggcctt gtgattggct taatgctgga
    22081 ttccattggt ccggcggcga aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt
    22141 cgatgtcggc tggccgggct cttcaccaat gacctgggcg tcgcaaattg cgctggtggc
    22201 gattccgatt gcgatattgg ttaacgtggc gatgttactg acccgtatga cgcgggtggt
    22261 aaatgttgat atctggaata tctggcatat gaccttcacc ggcgcgttgc tgcacctggc
    22321 aaccggttca tggatgatag ggatggcggg tgtggtaatt cacgcggcgt ttgtttataa
    22381 gctctgcgac tggtttgccc gcgatacccg aaatttcttt gagctggaag gtattgctat
    22441 tccgcacggt acgtcggcgt atatggggcc gattgcggtg ctggtcgatg ctatcatcga
    22501 gaaaatccca ggcgttaacc gaattaaatt tagcgccgac gatattcagc gcaaatttgg
    22561 cccttttggc gaacctgtca ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc
    22621 gggttacgat gtcaaaggtg tattgcagct ggcggtaaaa acggcggcag ttatgctgct
    22681 gatgccacgg atgattaaac ccatcatgga tggtttaacg cccatcgcta agcaggcccg
    22741 tagtcgttta caggcgaagt tcggcggtca ggagttcctg attggcctgg atcccgcatt
    22801 actgctgggg catacggcgg tggtatcggc aagcctgatt tttattccgc tcaccatttt
    22861 aattgctgtt tgtgttccgg gtaatcaggt actgccgttt ggcgatcttg ccactatcgg
    22921 cttcttcgtg gcgatggcgg tcgcggtgca tcgtggaaat ctgttccgca ccttaatctc
    22981 gggtgtcatc attatgagca tcaccctgtg gatcgcgacg caaactattg gtttgcacac
    23041 ccaaccggcg gctaatgctg gggcgttaaa agccgggggt atggtggctt caatggatca
    23101 gggcggttct cccattacct ggttactgat tcaggttttc tccccgcaaa atattcccgg
    23161 tttcattatt atcggcgcaa tttatctgac cggtattttc atgacctggc gtagagcgcg
    23221 tggttttact aaacaagaga aagccgttct cgcagaataa tttttacctg agggggaggt
    23281 tatccccctt aacaatcagg agtttttatg aaatcagtag tgaatgatac tgatggtatc
    23341 gtgcgcgttg caaaaagcgt cattcctgaa attaaacatc aggatgaggt gcgggtaaaa
    23401 attgccagct cgggcttatg tggttccgat ttacccagga tatttaaaaa tggtgcacat
    23461 tattatccaa taacgttagg ccatgaattt agcggctata ttgatgcggt gggatccggt
    23521 gttgatgatt tacaccctgg cgatgcggtt gcctgtgtgc cgttattacc ctgttttact
    23581 tgtccagagt gtttgaaagg gttttattcc cagtgcgcaa aatatgattt tattggctcg
    23641 cggcgtgatg gtggatttgc tgaatatatt gtcgttaagc gaaaaaatgt ctttgctcta
    23701 cccacggata tgcctattga ggatggggct tttattgagc cgattaccgt tggcctgcat
    23761 gcttttcatt tagcgcaagg ttgtgagaat aaaaacgtta ttattattgg tgccggaacc
    23821 attggcctgc tggctattca gtgcgctgtc gcgctgggag caaagagtgt gacggcgatc
    23881 gacattagct cagaaaaact ggcactggca aaatctttcg gtgcgatgca aacatttaac
    23941 agtagcgaaa tgagcgcgcc gcaaatgcag agcgttttac gcgaactgcg ctttaatcag
    24001 cttatcctcg agacggctgg cgtaccgcaa actgtcgaac tggcggtaga gattgccggt
    24061 cctcatgccc aactggcgct ggtgggcacg ttgcatcagg atctgcattt aacatcggca
    24121 acgtttggca aaatattgcg taaagagctg acggttatcg gcagttggat gaactattcc
    24181 agcccttggc cggggcagga gtgggaaacg gcgagccggt tgctgacaga acgtaagtta
    24241 agcctggagc cattaatcgc tcaccgtgga agctttgaaa gcttcaccca ggcggtgcgt
    24301 gacatcgctc gtaatgccat gccgggcaaa gtgttgctca ttccatgaaa ccgcgggcca
    24361 gcgtgatgct ggcccggtat tgtgcaaaac agatcattca ccaatggtcc cccttcgttt
    24421 acactagcca caattgaata tgggtaaatg acgatgaatt cattcgagcg aaggaataag
    24481 atcatccaat tagtgaatga acagggaacc gtgcttgttc aggatctggc gggagtattt
    24541 gctgcctcgg aagcgacaat ccgtgccgat ttgcgctttc tcgaacaaaa aggcgtggtt
    24601 acgcgctttc atggcggtgc ggcgaaaata atgtccggta atagtgaaac cgagacccag
    24661 gaagtcgggt ttaaagagcg atttcagctc gccagcgcgc caaaaaacag aatagcgcag
    24721 gcggcagtca aaatgatcca tgaagggatg accgttattc tcgacagcgg aagtaccaca
    24781 atgcttatcg ccgaaggatt aatgaccgct aaaaatatca cggtgattac caacagtctc
    24841 ccggcagcgt ttgccctttc cgaaaataaa gacattactc tggtcgtctg tggtggcacg
    24901 gtccgccata aaacgcgctc gatgcatggt tccattgccg agcgttcatt gcaggatatt
    24961 aatgccgatt taatgtttgt cggtgctgat ggtattgatg cggttaatgg tattacgacc
    25021 tttaatgaag gttattcgat tagcggggcg atggtcaccg ccgcaaataa agtgattgct
    25081 gttctcgatt catcgaaatt taatcgtcgc ggttttaatc aggtactgcc aatcgaaaaa
    25141 attgacatta ttgttaccga tgatgctgtg tcagaggtgg ataaattggc attgcagaag
    25201 acacgggtaa aattaattac ggtgtagtat tgttctgtat gccggataag gtgtttacac
    25261 cgcatccggc attacggatt ctccctggtt caagagagaa tagtttgatt aacgcaataa
    25321 tggacaatct ggtggtaatc gacaacgcaa cgccgccgga agtatttcaa tatggaagtt
    25381 ttgcccactc aacggttcgc catcaagatt aaaagtgatt tcgtgtgggg cttgtatatc
    25441 aaaccacgac gaagcgcctt cgataatatt cgggttatct tcgtcagatt ttaaggttga
    25501 tacgagagcc ggaagaattt catcgccggt aaaaatgcgc agttgcagca agccatcgtt
    25561 aattaacgcg ttcgggcaca attgttgacc gccaccggcc tgacgcccgt taccaatacc
    25621 aatgaccagg gcgtcacctt gccagtgaaa gttttcaccg cggatttcac aacggtccgg
    25681 ttgcagagta tccatgcgca ttaagccatg aatgatgtaa gagacgccac ccagcgcggc
    25741 ttttaatttt tccggcgttt ctgtggtaat acgcgtccca aatccgcctg tcgccatatt
    25801 aataaaacag gtttgtttgt tgacctgcgc catatctatc gcaatggcat taccggcaat
    25861 tgccagtttc agcgccttat ccagtgcctc aggaatccct acactggtgg caaaatcatt
    25921 ggcggttcct aatggcaaaa ttcccagcgc gggtatgtca tccccctcac actgaatcaa
    25981 cgccgtagaa acttcattaa tggtgccatc gccaccaccg gcaatcaccg ttgcgacgcc
    26041 caacttccgg gcctcctcta caaatcgtgc ggcatcacct ttctcccagg tgacccgcac
    26101 atggatcgtc attccttcct cacgcaacag cataattgct tcgcgcaagg gtagattgtc
    26161 agtactttta ccattaagaa tcagtaagct ggcgggaaat tctgccatga tcgtatgtgc
    26221 ctttatgatt ggtctgtaga tagtgtagag cagaaaacaa aaggtggagt cagaacagga
    26281 tgaaagtcgg aggataaaaa gtcagttcaa gtatggagga ttcagtggtt accaccagga
    26341 gctttcaatg gggacagcga atactaacgg ctgccaccga taaatttcaa aaaagagcat
    26401 atacctaata ttcaactaaa cagtggcatc ttcaatataa tatattaaag cccccatgga
    26461 gttaccctga agggcctcaa tgtccgtaat tcctacttat gtaggaaatg ttgtacagag
    26521 catttattat aatcctattc aattataata atcatgtcat tattatattt aaacactaga
    26581 gagtgtcgtt ggtatttaat gggggaaggt gagatgaaaa agatagctgc tatatcatta
    26641 attagtattt ttcttatatc tggttgtgct gtgcataatg atgaaacaag tatcggtaaa
    26701 tttggtcttg catataaaag taatattcag cgtaaactcg ataaccaata ctacaccgaa
    26761 gccgaagctt ctttagccag gggcagaata tctggtgcag aaaatatagt aaaaaatgat
    26821 gcagcccatt tctgtgttac tcagggcaaa aaaatgcaga tagttgacct gaagacagaa
    26881 ggtgcaggat tacatggcgt cgctcgtctg acattcaaat gtggagagtg agaatatttt
    26941 tttgtaagcg tcaaacatgc gcgttctggc tgtgcgtagc cggaacctgg gcgagcacga
    27001 taccgttacg tgaaaggcat tgtgctatga agggggattc tatcgatgtg gtcaatggaa
    27061 gacggttatc agggatcaat ccttctttac agcttatgta caaaaaataa gcccgtgtaa
    27121 gggagattac acaggctaag gaggtggttc ctggtacagc tagcatttta tggattatgt
    27181 ttttcagcga aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga
    27241 attttccatc cgggaaaaat aatcgaaatt aatcacttac cgtgggggtt acgcgtggtt
    27301 tccccggaga aattacgcat tagcagcgcg taatttagct caagatcctg cgggaccggg
    27361 agccacacag tataaccatc gcctggtgcg accggcatag cttcgccttt ggcgttttcc
    27421 atgtgctcaa gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag actgtcgcca
    27481 acggagaatt tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac
    27541 tcaccaacaa actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg
    27601 tcgtgagtat gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga
    27661 gtttccagca ggctggtatc gaacggtttt cccgcagcgg cgtcatcgat agctttgcgg
    27721 taaacctgtg cggtgcgtgc gcaatagtag aaagatttgg tacgaccttc gattttcagc
    27781 gaatgcacgc ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag
    27841 ttcatgatgt aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg
    27901 gcttcttcga tcataaacac tttgtcggtt ggcgcgccga tacccagcgt cggctcaaca
    27961 ttttgcaccg gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct
    28021 tcctggacgt tgtactccca gcggcaggcg ttggtgcagg tgccctggtt cgggtcgcgc
    28081 ttgttgatat agccagagag caggcagcga ccggagtagg ccatgcacag tgcgccgtga
    28141 acgaagatct cgatctccat atccggcacc tgattatgga tctcttcaat ctcttccagc
    28201 gacagctcgc gagagaggat cacgcgggtc agtcccattt gctgccagaa tttcaccgtc
    28261 gcccagttca cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca
    28321 cgcaccagca taatcagccc tggatcggac ataatcagcg catccggccc catttccacc
    28381 accggtttca ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc
    28441 acgacataaa actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg
    28501 tggttgaatt cgttgttgcg cacacgtagg gagtaacgcg gctggcccgc ataaacagca
    28561 tctgcgccat aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt
    28621 tccggtttaa acataattct ctcgttctga tgacaggtca gatccgcttc acctgatgaa
    28681 gcggttaggg gaggccccca ctttaagggc gggcattgta gcgctgtagg ggggatctgg
    28741 taaagcgccg gacgccaaca acccacgccc ggcatcgtaa ttacgtattg tctttcaccg
    28801 tctggcttgt gccattggct ttaacggagg caattccggt ttcacgagat tgcgcggtgg
    28861 cgtacatctg gctggagccg ataatttgat gattagcggc tttcagattg aaatagaatt
    28921 tgccgttgct tgccgttttt tctcatagcg ctcttcttgc gggctgttgc tacgcaccga
    28981 cgcgatgccc ttttccgcag aggcttttga ggtatacagc tcgctggtga ggatagtctc
    29041 accattgccc gcttttagca caaaccggaa ctgattatca ctgctcttac tgagttcaaa
    29101 ccaaccagcc ataatgactc cttgattgaa attgcttatc gaaaacagca tctaa
//
LOCUS       DAJTUN010000040        28568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTUN010000040 DAJTUN010000000
VERSION     DAJTUN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.040"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..910)
                     /gene="glpQ"
                     /locus_tag="OO470_001679"
     CDS             complement(<1..910)
                     /gene="glpQ"
                     /locus_tag="OO470_001679"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6250955.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDA"
     gene            complement(915..2273)
                     /gene="glpT"
                     /locus_tag="OO470_001680"
     CDS             complement(915..2273)
                     /gene="glpT"
                     /locus_tag="OO470_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6250956.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2546..4174
                     /gene="glpA"
                     /locus_tag="OO470_001681"
     CDS             2546..4174
                     /gene="glpA"
                     /locus_tag="OO470_001681"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6250957.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4164..5423
                     /gene="glpB"
                     /locus_tag="OO470_001682"
     CDS             4164..5423
                     /gene="glpB"
                     /locus_tag="OO470_001682"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6250958.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5420..6610
                     /gene="glpC"
                     /locus_tag="OO470_001683"
     CDS             5420..6610
                     /gene="glpC"
                     /locus_tag="OO470_001683"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6250959.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            6803..7753
                     /locus_tag="OO470_001684"
     CDS             6803..7753
                     /locus_tag="OO470_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6250960.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7794..8264)
                     /locus_tag="OO470_001685"
     CDS             complement(7794..8264)
                     /locus_tag="OO470_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6250961.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8319..9521)
                     /locus_tag="OO470_001686"
     CDS             complement(8319..9521)
                     /locus_tag="OO470_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6250962.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(9608..10150)
                     /locus_tag="OO470_001687"
     CDS             complement(9608..10150)
                     /locus_tag="OO470_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6250963.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10429..10854
                     /gene="nudI"
                     /locus_tag="OO470_001688"
     CDS             10429..10854
                     /gene="nudI"
                     /locus_tag="OO470_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6250964.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(10893..11495)
                     /locus_tag="OO470_001689"
     CDS             complement(10893..11495)
                     /locus_tag="OO470_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6250965.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            11803..12942
                     /gene="arnB"
                     /locus_tag="OO470_001690"
     CDS             11803..12942
                     /gene="arnB"
                     /locus_tag="OO470_001690"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6250966.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            12946..13914
                     /gene="arnC"
                     /locus_tag="OO470_001691"
     CDS             12946..13914
                     /gene="arnC"
                     /locus_tag="OO470_001691"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6250967.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            13914..15896
                     /gene="arnA"
                     /locus_tag="OO470_001692"
     CDS             13914..15896
                     /gene="arnA"
                     /locus_tag="OO470_001692"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6250968.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            15893..16783
                     /locus_tag="OO470_001693"
     CDS             15893..16783
                     /locus_tag="OO470_001693"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6250969.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            16783..18435
                     /gene="arnT"
                     /locus_tag="OO470_001694"
     CDS             16783..18435
                     /gene="arnT"
                     /locus_tag="OO470_001694"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6250970.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18432..18767
                     /gene="arnE"
                     /locus_tag="OO470_001695"
     CDS             18432..18767
                     /gene="arnE"
                     /locus_tag="OO470_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6250971.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            18767..19153
                     /gene="arnF"
                     /locus_tag="OO470_001696"
     CDS             18767..19153
                     /gene="arnF"
                     /locus_tag="OO470_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6250972.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19147..19419)
                     /gene="pmrD"
                     /locus_tag="OO470_001697"
     CDS             complement(19147..19419)
                     /gene="pmrD"
                     /locus_tag="OO470_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6250973.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19524..20879)
                     /gene="menE"
                     /locus_tag="OO470_001698"
     CDS             complement(19524..20879)
                     /gene="menE"
                     /locus_tag="OO470_001698"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6250974.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(20876..21838)
                     /gene="menC"
                     /locus_tag="OO470_001699"
     CDS             complement(20876..21838)
                     /gene="menC"
                     /locus_tag="OO470_001699"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6250975.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(21838..22695)
                     /gene="menB"
                     /locus_tag="OO470_001700"
     CDS             complement(21838..22695)
                     /gene="menB"
                     /locus_tag="OO470_001700"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6250976.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(22710..23468)
                     /gene="menH"
                     /locus_tag="OO470_001701"
     CDS             complement(22710..23468)
                     /gene="menH"
                     /locus_tag="OO470_001701"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6250977.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23465..25135)
                     /gene="menD"
                     /locus_tag="OO470_001702"
     CDS             complement(23465..25135)
                     /gene="menD"
                     /locus_tag="OO470_001702"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6250978.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25224..26519)
                     /gene="menF"
                     /locus_tag="OO470_001703"
     CDS             complement(25224..26519)
                     /gene="menF"
                     /locus_tag="OO470_001703"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6250979.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(26598..26903)
                     /gene="elaB"
                     /locus_tag="OO470_001704"
     CDS             complement(26598..26903)
                     /gene="elaB"
                     /locus_tag="OO470_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6250980.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(26958..27419)
                     /locus_tag="OO470_001705"
     CDS             complement(26958..27419)
                     /locus_tag="OO470_001705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6250981.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27484..28401
                     /gene="rbn"
                     /locus_tag="OO470_001706"
     CDS             27484..28401
                     /gene="rbn"
                     /locus_tag="OO470_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6250982.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         6874 a         7730 c         7161 g         6803 t
ORIGIN      
        1 gggcatcttg caccatgcca gtgagtttga tattacccgg ctgcgatgtc tcctcaatca
       61 acatatggta atccggacca ataccatctg catattccgc cacctgtttc atggctcccg
      121 gcttaaacat ccagtcgtag ttgtaattaa cccagcttcc atccggctgt ttctgctgcg
      181 tttcattcca gtcggtatag gcaatcagct gtaccagatt gagctccatg cccattttgg
      241 gttccagctc attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt
      301 tatcgtcttt accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat
      361 ccttcccttc ctgatgatgg aaccacggcg ctttgatttc tggatagata ccgatatttt
      421 tcccggtaga gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgcaccc
      481 ggaagtcgga cttacccatt gggaaacgcc ccggataagt ctgtactttt ttaccgtttt
      541 caatatcgaa accttcggta aatttcagcg acttaatttc atccagcgtg aaatctatcg
      601 cgtagtaacg accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac
      661 gatcgagata atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct
      721 gttccagata atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg
      781 gcaaatatcc actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca
      841 ttgcactgct tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagct
      901 tcaatttcat gccattagcc tccgttgcgt ttttgcagta attgttcatg gcgacgtttt
      961 tcgccaatca tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc
     1021 ataaagccgc catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc
     1081 accgaaccgc ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt
     1141 ttcggtgcca gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag
     1201 ccgataacaa tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa
     1261 acgatagtcg cgatggtcac cagtgtcata aagaagacgc cggttgcccc acggttacca
     1321 cggaagactt tgtccgacat ccagccgcac agcagggtgc ccggaatacc cgcatactca
     1381 tagaagaagt aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa
     1441 gtcggtgacc agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg
     1501 atgtaccaca gcagtttgtt cggcagcacg tactgcatga agatttgctt cgcggtcagc
     1561 tcctgttccg ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc
     1621 ggcaagccac aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga
     1681 atggcgcaga aagcaggcat atagagcgcc gcatgccagt cattgaacca ggccatcccc
     1741 agcaggaaca gcagcggcgg aataccacca ccgacgttgt gcgcacagtt ccacactgac
     1801 acaatgccgc cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc
     1861 caccccatcc cctggaacca accgcagagg aacaacagta caaacatcac cgcaatgctc
     1921 gacgtcgccc atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct
     1981 gcgggcagga aaacgcgcgg attcgagcga tccgataccg aacccatgat gaatttcgaa
     2041 aatccataag caatcgagat ccccgaaagg gcaaaaccta aatcaccgcg tgagaatccc
     2101 tgctcaacca gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca
     2161 tagccaaaga atatccccag gaaaatttgc cagcgcaatc gacgataagt cggatcgatc
     2221 tccgcggcag gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgatagc
     2281 ctccgtggcc cgtggtctta tttatgatta acagcctgat tcagtgagag aacctgccgt
     2341 ttcttgagtt gccgcgatgt taagaaaaca ttcataaatt aaatgtgaat tgccgcacac
     2401 attattaaat aagatttaca aaatgttcaa aatgacgcat gaaatcacgt tttactttcg
     2461 aatcatgagc gaatatgcgc gaaatcaaac aattcatgtt tttactatgg ctaaatggta
     2521 aaaaacgaac ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat
     2581 tatcattggc ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct
     2641 gcgcgtgatt ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg
     2701 cctgctgcac agcggtgcgc gctatgcagt aaccgatgcg gaatcggccc gcgaatgcat
     2761 tagtgaaaac cagatcctga aacgcattgc acgtcactgc gttgaaccaa ccaacggcct
     2821 gtttatcacc ctgccggaag atgacctctc attccaggcc acttttattc gcgcctgcga
     2881 agaagcaggg atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc
     2941 cgttaacccg gcactgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg
     3001 tctgaccgca gcaaacatgc tggatgccaa agaacacggt gccgttatcc ttaccgctca
     3061 tgaagtcacg gggctgattc gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca
     3121 tctcaccggc gaaactcagg cccttcatgc acctgtcgtg gttaatgccg ctgggatctg
     3181 ggggcaacac attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc
     3241 gctgctgatc atggatcacc gcattaacca gcatgtgatc aaccgctgcc gtaaaccttc
     3301 cgacgccgat attctggtgc ctggcgatac catttcgctg attggtacca cctctttacg
     3361 tattgattac aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct
     3421 gcgtgaaggg gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc
     3481 tggcgtgcgc ccgctggttg ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg
     3541 catcgtgctg ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg
     3601 tggcaaactg atgacctatc ggctgatggc tgaatgggct accgacgcgg tatgccgcaa
     3661 actgggcaac acgcgcccct gtacgactgc cgatctggca ctgcctggtt cacaagaacc
     3721 cgctgaagtt accttgcgta aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt
     3781 ttatcgtcat ggcgatcgca cgcctgcctg gctgagcgaa ggccgtctac accgtagcct
     3841 ggtatgtgag tgcgaagcgg taactgcggg tgaagtgcag tacgcggtag aaaatttaaa
     3901 cgttaatagc ctgctggatt tacgccgtcg tacccgtgtg gggatgggca cctgccaggg
     3961 cgaactctgc gcttgccgcg ctgccggact gctgcaacgg tttaacgtca cgacgtccgc
     4021 gcaatctatc gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat
     4081 cgcctgggga gacgcactgc gcgaaagcga atttacccgc tgggtttatc agggactgtg
     4141 tggtctggag aaggagcaga aagatgcgct ttgatactgt cattatgggc ggtggcctcg
     4201 ccggattact ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc
     4261 gtggtcaaag cgcgttgcat ttctcatccg gttcgctgga tttgctgagc catctgccag
     4321 atggtcaacc cgtggcagac attcacagtg gactggaatc tttgcgtcag caggcacctg
     4381 cacatcctta ctctcttctc gggccacaac gcgtgctcga tctcgcttgc caggcgcagg
     4441 cattaatcgc tgaaagcggt gcgcaattgc agggcagcgt agaactggct caccagcgga
     4501 ttacgccgct cggcactttg cgtgcaacct ggttaagttc gccagaagtt cccgtctggc
     4561 cgctacccgc gaagaagata tgtgtagtgg ggataagcgg gctgatggat ttccaggcgc
     4621 atcttgcggc ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc
     4681 tgccggaact ggatgtcctg cgcaataacg ccaccgaatt tcgcgcggtg aatatcgccc
     4741 gtttccttga taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca
     4801 atacctgcga aatgatcctg atgcccgcct gcttcggcct ggccgatgac aaactgtggc
     4861 gttggttgaa tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg
     4921 tgctgggcat tcgtctgcaa aaccagttac agcgccagtt tgtgcgccaa ggtggagtgt
     4981 ggatgccggg cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct
     5041 ggacccgcaa tcacgccgat attccgctac gtccacgttt cgcggttctc gccagcggca
     5101 gtttctttag tggcggactg gtagcggaac gtaacggcat tcgcgagccg attctcggcc
     5161 ttgatgtgct acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc
     5221 aaccgtggca gcagttcggt gtaaccactg atgagacgct acgcccgtcg caggcagggc
     5281 aaaccattga gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc
     5341 agggatgcgg cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc
     5401 aacgcgcagg aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt
     5461 ctgcaccacc gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc
     5521 cgggccggat ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa
     5581 atattgcatc aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga
     5641 tattatccag cgcgcgcggg cgaaatatga caccacgcgc ccgtcgctgc gtaattttgt
     5701 gttgagtcat accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac
     5761 cgctacctcg ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg
     5821 tcgcacgcta ccgaaatact ccttcggcac gttccgtcgc tggtatcgca gcatagcggc
     5881 tcagcaagca caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa
     5941 ccatccgcag ttaggtaaag atttaattaa agtgctcaac gcaatgggta ccggtgtaca
     6001 actgctcagc aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa
     6061 agcacgcaaa caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg
     6121 cattccggtg attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga
     6181 agtgctgaat gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct
     6241 gtggcgcaag ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt
     6301 ttatcacact ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt
     6361 gcgtaacatc ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg
     6421 tacttacggt ttcaaaaaag agaactaccc cacctcacaa gccatcggcg caccactgtt
     6481 ccgccagata gaagaaagcg gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg
     6541 gcagattgag atgtccacaa gtcttcgctg cgaacatccg attacgctac tggcccaggc
     6601 gctggcttaa actcccttct gatgcccggt aagcatgtgg ttaccgggca tttttgcgta
     6661 cacgattccg tgcccaatgt atgcgttgca acgcagtgaa aattcctctg aaaacgtctc
     6721 gcaaaggctg aaactggcag atgtcaaagg cctgggataa ccgtaatgtc gcgtcatcat
     6781 aaatatcagg tgacggacaa ccatgaccga atcaacaacc tcctccccgc atgatgcggt
     6841 atttaaaacc tttatattca cacccgaaac cgcacgggat tttctcgaaa tacatttacc
     6901 agaaccactg cgcaagcttt gcaacctgca aaccttacgc ctggaaccca ctagttttat
     6961 tgaaaaaagt ttacgcgctt actactcgga tgttttgtgg tccgtggaaa ccagcgacgg
     7021 tgacggctat atctactgcg tgattgaaca tcaaagctct gcagaaaaga atatggcttt
     7081 tcggccaatg cgctatgcca ctgccgccat gcagagtcac ctagataaag gctatgacag
     7141 agttccgctg gtggtgccat tgctgtttta tcatggcgaa acatcgccct acccgtactc
     7201 acttaactgg ctggatgagt ttgacgatcc gcaacttgcc cggcagttgt acaccgaagc
     7261 ttttccgttg gtggatatca ccatcgtacc tgacgatgag atcatgcaac atcggcgtat
     7321 agctctgctg gaactgattc aaaagcatat tcgcgaccac gatttaatcg gcatggtcga
     7381 caggatcacc acgcttttgg ttagaggctt cactaatgac agccagctac aaacactgtt
     7441 taattatctg ctgcaatgcg gcgatacctc ccgtttcacc cgttttattc aggagattgc
     7501 cgaacgttca ccactacaaa aggagagatt aatgactatt gctgaacggc tacggcagga
     7561 aggacatcaa attggctggc aagaaggtaa attagtaggt ttgcagcaag gtaaattaga
     7621 aggtttgcag gaaggtatgc atgaacaagc cattaaaatt gctttgcgca tgctggaaca
     7681 gggcattgat cgtgaccaag tgctcgcggc cacccagcta agcgaagccg atctggcagc
     7741 gaataaccac taattaacac aggcccacag ccgatcccca tgggcctttg atatcaataa
     7801 ctacctttta tgcgtggccc atttttgcct gatttaaaca tcgccagtcg ttgatccagg
     7861 gcatcgctgt agagcatcgt gtcaacgcca acagcgacaa agttcgctcc ccacgccagg
     7921 cattgctgcg ccatatcagg agccacagcc agaaaaccag ccgctttacc cgcagcacgg
     7981 atccgccgaa tgctggtttc aataattcgc tgcacttccg ggtgcccggc gttatccggg
     8041 tagcccaacg acgcagaaag atccgctggt ccaataaaca cgccatcaat cccttcgacg
     8101 tcgaggatct cgtccaggtt atccagtgcc gttttacttt ccacctgcac taacagacaa
     8161 agcgaatcgt tgacttgcgc catgtaattc tcaatgcgtc cccagcgcgc agcccgtgcc
     8221 acactggccc caacaccacg ctcaccgtag ggagggttgg acaaattttg ccggaggggg
     8281 aatgtgccgg tgcagtaaac cggcacagag tggcgaaatc agacggataa agtcatggac
     8341 tcaacaacct caatccagcc atgctcacta gcgatgtcct ggccgtttaa ccaacggcgt
     8401 agcatattca gtgccatcat tgcgcacact tcctgacgga tagccaggct gtagcgtgta
     8461 gtgctgaaac gcacacgcag agcgaaagtg ccgtctggcg tggctagcgc aaagttgaga
     8521 tgctcgttct cgaaacccga aacagccagt gccagcccgg caaaatggtt ggcccgccgt
     8581 tctgtaatcc agtgcgcagt ttgcgccagg gtttcttcct gtgaaggaat cacttcacac
     8641 gccagcaatg gagcacctgc gcgagaaagt tgcaaagcca ataaaccacc ggtgaattgc
     8701 tcgctcaacg tcaggctgaa ctggcggttt tgcaattcgc gactgatctg cgcgggcagt
     8761 ccttcggtgc cttcgaaaat cacgctctgt ccggcaacac gtttaacgtc cagccacagt
     8821 ttttccatcg cctgttgctc gcttgccggt ccggtgagtt tcagttcgat gatcggcatt
     8881 gaggagcgat agcccattgt tacgcccggc ggcagttgta gagtgtccag gctttgtgcc
     8941 agatcgcttt ccgaacgacc aaaggtagtt aaacgcagac aaaccggcgg ctgtggtaaa
     9001 gaaaaacgct cgcgcaggcg cggcaggatt tcgtgctcga ccatcacctt aaattctgac
     9061 ggtacgccgg gagtaaagaa catcaggcaa cgattaagct gcacggcaaa accacaggcg
     9121 gtgcctaccg ggttattgat aaattcagca ctggcaggca gctccgcttg tttacggttg
     9181 ctcggtgcca ttactcgtcc acgttcgtgg aaatagcgtt ccatctcttt gagccaggct
     9241 tcatgcagca ccaggccttc accttttgct gtcgcagcgg cgagtgcgct taaatcatcg
     9301 ctggtcggtc ccagcccgcc gttaacgatc agcacatcgg cgtgctgact acgttcgcga
     9361 agaatggtga ctaagtcatc aaggttatcc cccaccgtat tgcggcgaga taatggcagc
     9421 ccctgatgaa agaaaaaatc ggccagccag gcagcgttag tgtcaacgat ttgcccgtat
     9481 aacacttcat ccccggtgga tagcatttcc acttttaaca tggttatctc ctgcttttgt
     9541 tatcgaatca ctataacgca aagggagata aggcgtagta agaaactgaa tgccgcgccg
     9601 gaagggatca gaaactggcg ttaaccccaa catacaggcc gtcagccaca gcgttgtcgc
     9661 ggttaccgtc tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggac
     9721 gcataatggt gtaacgcgcg ccagcattcg cttcctcata actttgaata ccgctggaga
     9781 gcgaatccgg agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact
     9841 gcaaaccacc tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaca
     9901 cgccttttcc gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca
     9961 cgcccgcgac gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaaa gccaggcccg
    10021 tcgattcagt cccaaaaccg acaccaatat tggtgtattc tttacccgcc tggccgctga
    10081 tgctcattgc attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa
    10141 tttttttcat tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt
    10201 gtactgcctg gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac
    10261 taaatacttc ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa
    10321 aaataataat aagtgcagtt atcattgcaa aagagattac tgtcactttc acatatttct
    10381 gagttaccgt attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg
    10441 actattgtat gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac
    10501 gatcgcggcg ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa
    10561 cgaattgaag aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg
    10621 acagaaatca cgccgtggac cttcagcgat gatattcgca ccaagacata tgcagatggt
    10681 cgcaaggaag agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa
    10741 gtgaaaataa acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat
    10801 tatgatttaa atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga
    10861 tgacagccat cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga
    10921 taaactttgc ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatatc
    10981 gcatcacgct tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata
    11041 tttttatcag gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac
    11101 tgaagaagtc gtttatctac cgtcagcttt ttacccgcgg aaaaccaggt cgccgactga
    11161 atggtccgga cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg
    11221 ttgcccagtt cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag
    11281 cattgattgg ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc
    11341 tgctgtgcca gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag
    11401 gcggcatgag tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt
    11461 tttgacttca atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc
    11521 aaccagacaa ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa
    11581 tattaaaaat cctacgatgt cggcaaaatg cctcaaattt ttgccaaatg caaagcctaa
    11641 ataagaaaaa atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt
    11701 taatattcta tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag
    11761 caaatataag tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct
    11821 ttttcgcgac cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc
    11881 ggttggatca caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg
    11941 ggaaatcagc atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg
    12001 gcgttggaaa ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc
    12061 ctcaatatga tttccttgct gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg
    12121 ctgatggtca cgcctgaagc gatcgaatca gccattacgc cacgcactaa agccatcatt
    12181 ccggtgcatt atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt
    12241 tacggcatcg cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga
    12301 catattggcg caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt
    12361 gctgaaagtg gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg
    12421 aaatttcacg gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag
    12481 gctgaagtct taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc
    12541 ctgacacagt tagtcaaatt agagcacctc aacacccgtc ggcgcgaaat tgcccagcaa
    12601 tatcagcaag cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac
    12661 gttcacgcct ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc
    12721 gatgcgttga tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcacc
    12781 gctcacacac aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa
    12841 tggaatagcg aacgcatctg ttcgttgccg ctgttcccgg atatgactac cgccgatgcc
    12901 gaccgcgtca tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac
    12961 cctgttaaga aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa
    13021 ttaatcaggc gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg
    13081 attgatgacg gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag
    13141 aacagccata ttgtgtctat tttgattaac cgcaattacg ggcaacattc agcgattatg
    13201 gcgggattca gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat
    13261 ccgccagaag aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg
    13321 actgtacgcc agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac
    13381 cggcttattc agcgcaccac tggcaaagcg atgggtgact acggttgtat gctgcgcgcc
    13441 tatcgccgtc atattgtcga tgcgatgttg cactgccatg aacgcagcac ctttatcccg
    13501 attctggcga atatcttcgc ccgccgtgca attgaaattc cagtacatca tgccgagcgt
    13561 gagtttggtg aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg
    13621 acctgcctta ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt
    13681 ggaggtttta gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg
    13741 gcggcagaag gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt
    13801 atcggcatgg gattactcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc
    13861 ccccgctatt ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa
    13921 ccgtcgtttt tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg
    13981 gttacgaaat tagcgccatt tttacccata ccgataatcc cggtgaaaaa gccttttatg
    14041 gttcggtggc tcatctagcg gcggaaagag acattccggt ttatgcgccg gataacgtta
    14101 atcatccgct gtgggtggaa cgcattgccc aactgtcacc agaggtgatt ttctcttttt
    14161 attatcgcca tcttatttgc gacgagattt ttcagctcgc tcccgcaggt gcatttaatc
    14221 tgcacggttc gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga
    14281 acggtgaaac ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg
    14341 ccattgtggc gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc
    14401 ataaattgtg ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg
    14461 gtaatattct ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt cgcagaacgc
    14521 cggatgacag tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg
    14581 ccgttgccga tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct
    14641 ggtcgtcgcg tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg
    14701 cgccactgct gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg
    14761 acggcattac tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac
    14821 gcttgaatag ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg
    14881 tgaatggctt tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag
    14941 tttacggtct ggatattggc agcgatgcga taagccgttt tctgaatcat ccacattttc
    15001 actttgtcga aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat
    15061 gtgatgtcgt cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc
    15121 tgcgcgtatt tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt
    15181 accgtaagcg aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat
    15241 acttcgatga ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt
    15301 attcggtgtc aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt
    15361 tacagttcac cctcttcctc ccgtttaact ggatgggacc acgactggat aaccttaatg
    15421 cagcgcgaat tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt
    15481 caccaattaa gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg
    15541 gtatcgaggc gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta
    15601 tcaacattgg caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg
    15661 cgagcttcga aaaacatccg ctgcgccatc atttcccacc gtttgcgggc tttcgcgttg
    15721 tcgaaagtag ctgctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca
    15781 tccgcaatgc ccaccgctgc ctggactggg agccgaaaat tgatatgcag gaaaccatcg
    15841 acgaaacgct ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa
    15901 agtaggctta cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccgcgtct
    15961 gctggaaatc ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga
    16021 caatatgggc cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg
    16081 ctcaaacgcg gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg
    16141 taaagagatt ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt
    16201 cggtctgcac gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca
    16261 aacaatgatc gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc
    16321 ggtaacctgt tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga
    16381 agcgttccat ttgcgctaca acagcgattg tcgtggggcc ataccgttcc gtccactgct
    16441 tgaatcagga aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt
    16501 gattggtcgg gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg
    16561 agataaaggc acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca
    16621 taattttgtg gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag
    16681 cgaactgtta tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg
    16741 tgaaggctgg ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac
    16801 cttatcggca tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg
    16861 ctctggcaac ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc
    16921 gactggattg tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac
    16981 tggattaaca gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc
    17041 gttatctttg cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg
    17101 cgcgataaac gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat
    17161 gccatcggca cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg
    17221 tgcagcttct ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta
    17281 ctgctgggaa tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg
    17341 ccggtattaa gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt
    17401 tacggctggc tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata
    17461 gcgcagcggg agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt
    17521 gcactagatg atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc
    17581 ggtagcctgc cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc
    17641 aagcattccg caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc
    17701 gtcgctaaag gcaaattgcc cacctatatt ctttcttgct ttgcacctct ggcaatgctg
    17761 ttggcgcatt acgcgttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc
    17821 tggattaaca tcgcttttgg cgtcactggc attattgcca catttgtgat ctccccctgg
    17881 ggaccaatga acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg
    17941 tcgatttttt cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc
    18001 tggtctttcg ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct
    18061 gacagagtca tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt
    18121 cagccaagcc gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc
    18181 ctgcaacgcg atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat
    18241 tatccggatg cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa
    18301 catcgtcagg aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac
    18361 agtctcgcca ttccgcccgc agatgccatc gttcgtcagg agcgtctggt gctgattcag
    18421 tatcgtccca aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc
    18481 agttgtgtca gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg
    18541 tgctgtggct gggactggcg ctggcttgtc ttggtcttgc catgatgctc tggctgctgg
    18601 tctcgcagaa cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg
    18661 tgacgctggc tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg
    18721 tggcgttcat tattggtggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatatg
    18781 gggattattc agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcaag
    18841 tcatctgccg ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct
    18901 ggatgcccgg atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta
    18961 taaaacgcta cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt
    19021 gctggtatgg attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc
    19081 actactggga gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa
    19141 acaacgttac tgagttttcc ctgccgctgt acactgccgt tcccactcat cggggctata
    19201 actgcttgcc gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc
    19261 attctgcaaa ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc
    19321 gatcattttt atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt
    19381 attgcagcac gattttttgt gccagccatt ccattgcatt atcctgtttg ctaagagttt
    19441 tcacatcaat ttacagatta acagataaac tctttatcaa cggcaatgga atggcagaaa
    19501 tgagaagtga aacggagggt taattattgc tgacgttgca cccactcttt tagcgcctga
    19561 cgtgaaattt taataccgcc gtttttcagc tccggcggta aagtcagcca gcgcaccggt
    19621 tgttgaaaac gagccagctt atctttcacc cattcgccaa gatcaacggt ttgctggtca
    19681 tactccacca ccgctaccgg ccgatgacca aactccttgt cggcaacgga aacgataaac
    19741 acctgcaaaa ccgcaggatg tgcagcaata acacgctcga cttcttccgg ctgaataccc
    19801 tcaccgccac tgaagaacag attgtctaaa cgtccgacaa tggtcagctt gccattatgc
    19861 atctcaccgc gatcgcgcgt agcgtaccag ccttcgtcat taaccagtga aaccagttgc
    19921 ccgttacgcc agtaaccttc tgccatactg gcagcccgca gccacacttc attattaacg
    19981 attttcactt cccgacccgg cagcggcaaa ccaacgtctg ccaggccgtc ggcttctttc
    20041 gcacataccg tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc
    20101 tcgcgcgcct gttccgtcaa ctcgaccggg atagccgcgc cgccaagtaa caccgctttc
    20161 agggaaacgg aactgcggtt aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg
    20221 tgagtacagc ctgccagcat ttgctccaat ggctgtttat cacgcaccgt catccgcgca
    20281 ccagcgtata accagcgcca cataattccc tgaccggaga cgtgaaacag cggtaaagag
    20341 agcaaccagt catcgtgatc gccaaacgga atcagcgata acacaccttg cgcactggca
    20401 agatgggcct gataagtatg gacagcggct ttcggcaaac cggtagaacc cgaggtcaac
    20461 gtcattgagc acagacgcgt cggctgccac gcagcggcat gtacgccttc aaccagctga
    20521 atgtgcagcg acgttaatgc cggaaacgtg ttttccccat ccggcaccag cgcaaattgc
    20581 agcgtcagat tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg gttcacgggc
    20641 aacacccgcg ccccgcattg cagtaacgcc agccaggcga gcagcgtttg cggcgtattc
    20701 cacgcccgca acatcacgcc gctgccctca accaccccct gcgccgcaaa gcccgtagct
    20761 aactcatcga cgcgagcaca aagctcgcgc cagttgagtt gctcgtcatt aagacgtaag
    20821 gcgatggctt ctccccgcac ttgccgccag tgacgccacg gccagtcaga gaagatcata
    20881 acaactgctc ctgtacatca gcgtccacta acggcagtgg gctccccggc cagcgacgaa
    20941 cctgctgcgc ctgcatcaga tccagcgtgt ccagtcccgg aatggtgtct ggcgttaacc
    21001 aggcagcaat ccgcgccagt tgcgttagac ctaagctcga ctcaatggaa gaactgatca
    21061 ccgccgtcag ccccagcgca tgcgccgcct gtacctgctc gcgtactttt tccagactgc
    21121 ccgtgagcgt gggtttgata actaccgcgc gcacgccctc ttcagccaca aaggcaaaat
    21181 ccggctcgcg caggctttca tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc
    21241 gcgaatcatc gcgggttttg cacggctctt cgagaaacgc gatgcggtgg cgataatccg
    21301 ggttaacata tttggcaaac tgctgacctt tcagcggtgt ccaggcgcga tttgcgtcaa
    21361 gacgcaaatg cagatccgga attgcctcca gcaacagatt caccaccatg ccgtcgcgca
    21421 ccgcttcgta caatcccact ttgaccttcg ccactttctc gcccggcata tcggcaagtt
    21481 tgaggatcag atcgtccgga tcgccattac acagcggtgc cgcacggtag ttggctgctt
    21541 gcggcaacgt atctgtcagt tctgccaatg cacagcttac gccaaaggcc acggaaggca
    21601 tctgcggtag ctcgcaatcg cctgccagcc agttatttac ccaggcaagc agcacgcttt
    21661 gcgcctcttc ccagttttcc tggctgaagc ccggcagtgg ggagatctcc ccccaccctt
    21721 cgcgctcgcc ctcacgcagg caaacgtaca gcccgtcgcg ggtttttaac cgcctgtcgc
    21781 gcagaaccac ccccgcgtcc atggggatct gccagcggta tacctgcgcg ctacgcatta
    21841 cggattccgt ttaaatttgc tgaaatcagg ctgacgtttc tggttgaaag cgttgcgacc
    21901 ttcctgacct tcttccgtca tgtagaacag catggtggcg ttgcccgcca gctcctgcag
    21961 ccccgcctgc ccgtcacagt cggcgttcag cgcagctttc aggcagcgca gcgccatcgg
    22021 gctgttttgc agcatttcgc ggcaccaacg gacggtttct ttttccagat ccgccagcgg
    22081 taccacggtg ttgacaaggc ccatatccag cgcctgtttt gcgtcgtact gacggcacag
    22141 gaaccagatt tctcgcgctt ttttctgccc gacgatacga gccatgtagg aagcgcccca
    22201 gccgccgtcg aaggaaccga ctttcgggcc agtctgaccg aagatggcat tatctgctgc
    22261 gatagtcagg tcgcacatca tgtgcagaac gtgaccaccg ccgatggagt agccagccac
    22321 catcgcgaca accggtttcg gacaggtacg gatctgacgc tggaagtcca gcacgttcag
    22381 gtgatgtacg ccggaatcat ctttatagcc gccgtaatca ccacgcactt tctggtcacc
    22441 accggagcag aacgctttat cgcctgcgcc agtcagaatg atcacgccga tattgtcgtc
    22501 ataacgcgca tctgccagtg cctggatcat ctcttttacc gtcagaggac ggaaggcatt
    22561 gcgcacctgc ggacgattaa tggtgatttt tgcgataccg tcggtggatt tttcataacg
    22621 aatgtcctcg aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaag gcattgcttc
    22681 atcaggataa atcatattgt gtcctttagt cagaaacgca agatctgcgc cagacttgcg
    22741 attacgccag cgggattttc ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcag
    22801 tcggcagcca gttccgccgc cagggcgcgg aatttgctgt cacgttcacc acataaataa
    22861 taaaacgcaa atgtgcgggc gctaaggtta gcacgtaaat caggctggac ggcgagagaa
    22921 gtcgcctcca gcatggcggc aagggttgcg ccattattgt tgctgcgcag cgccaccagc
    22981 tcccggcgtt gatcgtcatt gagcgaggca aaaacaggct gttgatacca gtcggcaaat
    23041 accgccgtta acggttctgt gcgaaaacgc tgcgcccatt ggcgatcgga acgctgacgt
    23101 tccgcacgtt gttcagcatt ttgcagcccc ggatgcccgc cttcgacaat aaccccacaa
    23161 agccccgcca gcccctggca agccgccatc atcgccaccc gtccgccaag cgagtacccc
    23221 accagccaga agtcaaggat gttgtaacta accaaggttt tacgcagtaa gtcggtgaca
    23281 tcatcaaatc catcgacgct aatcgccgcc gaaccaccat gacctaggag atcaacatac
    23341 aaccgtgagt agtcggcaaa cgcctcgccc acttcttgcc attcgtggca atcgccggaa
    23401 aaaccgtgca aaaacaccag ccagggtaaa cctagttttc cgtgttttgc ctgcgcgtgc
    23461 aggatcataa atggcttacc tgcgccagaa gttgctggag cgtttgcgca ccatcggtgt
    23521 cgttaaccac catttcaatc accgtggtgg ttggcgtgcg ccaggcgtcg gcaaatgccg
    23581 tttcaagttc ctgccagttt tgcggatgat gatatttcag ctcgaacatc gcggcggcgt
    23641 gctcaaaatg gacgttttgc ggcatcagat agaaacgctc acgctcgctt tgcggcgttg
    23701 gcaacagcga gaaaatttgc ccgccgttgt tgttcaccac aattaatacc agcggcgcag
    23761 aaacctgacg caataacgcc agcgcgttga gatcgtaaag cgcggagaga tcgcccacaa
    23821 tcgccagcgt cggtttgccg cttgcccgct gaacgcctgc ggcggtcgaa agcagtccgt
    23881 cgataccgct ggcccctcgg ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg
    23941 catcaatcag acgtaccacc aggctgttac caacaaacaa ttgcccctgt tcaggcagat
    24001 agtcgctgat gcgatgcgcc agttgcgctt cgccaaacgc atcacggcgg gcaataaccg
    24061 cctgcattgc ctgttccgcc aggcgcggga tttcaacgca ccagggctgg cgtttttctg
    24121 ccggatgcag ctccagccag tcggcaatat tggcaattaa gcgacgtccg cgatggtgtg
    24181 ccggatcaag tcgcccttca atgtcatcaa caatccagta ctcttctggt tcacagcttg
    24241 cctgccattg caggagccgt ttccccgtca ggctacttcc cagttgcacc acaatttgcg
    24301 cctgctgcag ctcgctggtc gctttggcat tgcctaacca aagatcggca cacggcagcg
    24361 gctgcccggt ttgtgacagc acatcgccaa tcagcggcca gccaagagtt tgcgcccaca
    24421 gggcaacttt tttgccctct tccgcactca tgcgcccggc aaccaccacg ccgcgctttt
    24481 gtcgccagaa gaaccagtcg cgctgttttt cactttccag acgaggcgct tcacgcagcc
    24541 acggtttgtc gtcctgccac cagtcaccca gacgctgttg ccagctaagc ccggtatcgt
    24601 ccatttcgcc atacagcggt tcagcaaacg ggcagttgat atggactccc cccgcatgaa
    24661 gcgtaccgag agcgtggtcg atggtagaaa ccagccaacg tgcggggata tcctgggtcg
    24721 ggcgcggcaa tgaaatactg tgcgtggggt gagaggcgaa cattcccggc tggcgaattg
    24781 cctgattcgc gccgcagtca attagctccg gcgggcgatc ggcggttaag agaatcagtt
    24841 tttctccggt taacccggct tcaatcagtg ccggatagag atttgccacc gccgtgccgg
    24901 aggtcacaat caccgccacc ggctgcttgc tgactttcgc cagccccagc gccagatgcc
    24961 ccaacccacg ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta
    25021 acgttaacgg tgtagaacgc gagcctgggg cgatacagat gtgtctgacg ccgtgacgcg
    25081 ttaatgcttc cagaatgacc gccgcccagc gtcggttaaa tgcgcttact gacatgagtt
    25141 tgtccggtat caatattgcg gctaagtata aggagctaca aaaatagatt attgatatga
    25201 atcggtaatg atgcgactca ttactattcc atttgtaata aagtacgcag ccctgccgct
    25261 ttgttgtcga tttcctgcca ctcttgctcg gggtcggaac cacggacaat gcccgcgcca
    25321 gcatataatc gcacgacatt gccgctaatt tttgctgagc gcagggaaac gcagaattcg
    25381 ctttgttgta atgagagata gcccgcagaa ccggcgtacc attctcgggt gaacggttcg
    25441 tgacgggcga taaactgtcg cgccagatcg cgcggtaagc cagcaactgc tgccgttggc
    25501 tgcaactgat gtaaacagat cacatcatcc gctttgttga gtgaagtcca gatacagcgg
    25561 cgaagatgct gcactttacg cagacgcagt acctgcggcg gtaaaacatc cagcgtctgg
    25621 gtatcggcct gtaatcgttg gcagatatct tccaccacca gcatgttctc gcgctggttt
    25681 ttatcatccg ccatcagcca ctctcctaac tgctgcgcct gcttatcatc aggatgattt
    25741 gctactgttc ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg ccataaccgt
    25801 tccggtgaag agccaagaaa agcattttcg ccatcaaagg ccatgtaaaa atggtagcaa
    25861 ttcagattca gtcgacgact ggcagccatc atcgccgccg cgttgaccgg acttgcgaaa
    25921 tgcaggtcag ttgcccgagc gagcaccact ttgtcgagct caccttcggc gatggttttc
    25981 gttgccagtt cgattaattg cgtccagccc gttttgtccg gccagtgttg ttctcgcgtg
    26041 gtggttaaat gtaacccagg caagggcttg atactcacca gtgtggcgat aaattctttt
    26101 gcctgaatcg catcgtgctg aagggagctt tcgctgaata gcgtcagccg cagcgtggct
    26161 ttaccgccac agcgtcgcca ttccaggcgg ggtaaaagta aattgccctg cgacgggtca
    26221 aaagcattca gcccccaaat gcgtaagtca gcgtgttccg ggtgctggcg aagaaaacgt
    26281 tgtgcctggt ccaacgacgt aaaacgggta atcgcgccca ggacggcagc ttcttcatca
    26341 ccattacgtt gttgccagta gaattgcggg tacgtttgct gactggccag ccagctcaag
    26401 gcatcaaaag cgtctttgag agggaaagga atatcgataa cccgaatgcc gggtgtcgcc
    26461 ggaatctctt gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg
    26521 cggacctctc cctgttaaaa gcctcacatt atacggggta ctacaaaaaa aatgcagtac
    26581 cccggtgtag ggaggtttta acggcgtgcc agcaacagtc ctagtaccag cccgacggcc
    26641 gcgcccacac caattccttg ccagggtttt tcgtggacgt agtcatcagc acgataaaca
    26701 gcctgcttcg cccgatagta ataactgtct gaagcctggc taacccgttt tttcacatca
    26761 tccagcgctt tttctgcatg cgctttcagc tcaacatatt tctgatcggc gggatcgcca
    26821 gaggagcgga gcacctcttc cagtgtttca ctaagcagcg tcaggtcgtc atcgatacgt
    26881 gtatcaccaa actgattaga cattctcgtt ctccatttgc gtaaaacctg tcactaacta
    26941 tagacaagag ttgttgatta cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata
    27001 ccatcttctt catagacctc cgtcacaggg ataaaaccga aactctggta gaagttttgc
    27061 agatgcgcct gcgccccgag gtaaacaggc ttatcaggcc agtgatgcgt acatgtttcg
    27121 agtgttttac tcatcagctg ctgccccact ttttcaccac gcaacgcttc actgacaatc
    27181 acccgaccta taacgaccgg ctcaagatca tcatcacttt tcagaatcct cgcatacgcc
    27241 accagttcat catttttcca accgagaata tggcgattat cccccgtcaa atcatcacca
    27301 tcgatatcct gataagggca attctgctcg acaacaaaca ccgcgcaacg cagttgtaat
    27361 aaggcataaa gctgggaaac cgataattca gagtgatgca gatcttgcca ttcaatcata
    27421 atgctctcct tttagatgta tgccttatac tagccggaat attttttgaa acgtgatgaa
    27481 ctcatggaat taattttttt aggtacttca gccggtgtgc caacccgcac gcgcaatgtc
    27541 acggcaatat tgcttaacct gcaacatccg acccagagcg gactttggtt gtttgactgc
    27601 ggtgaaggca cccagcatca gctactgcat accgccttta accctggaaa actggacaag
    27661 atttttatca gtcaccttca tggcgatcat ctttttggtt tacccggctt gctgtgcagt
    27721 cgttctatgt caggcattat ccaaccctta acgatttatg gtccgcaagg tatccgtgaa
    27781 tttgtggaaa ccgcgctgcg gattagcggc tcatggaccg attatccgct ggaaattgtc
    27841 gaaattggcg ctggcgaaat tctcgatgat ggcctgcgca aagtaaccgc ttatccgctg
    27901 gaacacccac tggaatgtta tggctatcgt attgaagaac atgataaacc gggtgcatta
    27961 aatgcccagg cattaaaagc tgctggcgtg ccgcctggcc cactgtttca ggaattaaaa
    28021 gcgggcaaaa caatcacgct ggaagatgga aggcagatta acggcgcaga ttacttagct
    28081 gctccagtgc caggtaaagc gctcgctatt ttcggcgata ccggcccctg cgatgccgca
    28141 cttgacctgg ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga tataaccatg
    28201 gaagccaaag ccaatagtcg cggccatagc tctacacgcc aggctgcgac actagcccgt
    28261 gaggctggag tcggcaagct aatcattacc cacgtcagct cgcgctatga tgacaaaggt
    28321 tgtcaacacc tgttacgtga atgcaggtca attttcccgg cgactgaact ggcgaatgat
    28381 ttcaccgtgt ttaacgttta actctgctta ttattaaaaa cagggtgaaa cttgccctgt
    28441 tatagcaacc cgcgctacat atatccaccc acacaggcat attgagcgat ttttgatatt
    28501 tattcatctc cttacgcact atattcagag caataagttg cctcttttca tcttgattca
    28561 gcacaacc
//
LOCUS       DAJTUN010000041        28174 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTUN010000041 DAJTUN010000000
VERSION     DAJTUN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.041"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..722
                     /locus_tag="OO470_001707"
     CDS             <1..722
                     /locus_tag="OO470_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6250983.1"
                     /translation="NILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEG
                     NPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMG
                     FTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNV
                     VDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEG
                     LKKQFDPNGIMNTGTIYPIEK"
     gene            744..2153
                     /locus_tag="OO470_001708"
     CDS             744..2153
                     /locus_tag="OO470_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6250984.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2131..2910
                     /locus_tag="OO470_001709"
     CDS             2131..2910
                     /locus_tag="OO470_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6250985.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2907..3767
                     /locus_tag="OO470_001710"
     CDS             2907..3767
                     /locus_tag="OO470_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6250986.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3915..4490)
                     /locus_tag="OO470_001711"
                     /pseudo
     CDS             complement(3915..4490)
                     /locus_tag="OO470_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4507..4767)
                     /locus_tag="OO470_001712"
     CDS             complement(4507..4767)
                     /locus_tag="OO470_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6250987.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4758..6029)
                     /locus_tag="OO470_001713"
     CDS             complement(4758..6029)
                     /locus_tag="OO470_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6250988.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6107..6469)
                     /gene="queD"
                     /locus_tag="OO470_001714"
     CDS             complement(6107..6469)
                     /gene="queD"
                     /locus_tag="OO470_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6250989.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6788..8587
                     /gene="cysJ"
                     /locus_tag="OO470_001715"
     CDS             6788..8587
                     /gene="cysJ"
                     /locus_tag="OO470_001715"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6250990.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8587..10299
                     /gene="cysI"
                     /locus_tag="OO470_001716"
     CDS             8587..10299
                     /gene="cysI"
                     /locus_tag="OO470_001716"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6250991.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10373..11107
                     /gene="cysH"
                     /locus_tag="OO470_001717"
     CDS             10373..11107
                     /gene="cysH"
                     /locus_tag="OO470_001717"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6250992.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11371..11523
                     /locus_tag="OO470_001718"
     CDS             11371..11523
                     /locus_tag="OO470_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6250993.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11717..12808
                     /locus_tag="OO470_001719"
                     /pseudo
     CDS             11717..12808
                     /locus_tag="OO470_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12954..13991)
                     /gene="iap"
                     /locus_tag="OO470_001720"
     CDS             complement(12954..13991)
                     /gene="iap"
                     /locus_tag="OO470_001720"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6250994.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14243..15151
                     /gene="cysD"
                     /locus_tag="OO470_001721"
     CDS             14243..15151
                     /gene="cysD"
                     /locus_tag="OO470_001721"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6250995.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15153..16580
                     /gene="cysN"
                     /locus_tag="OO470_001722"
     CDS             15153..16580
                     /gene="cysN"
                     /locus_tag="OO470_001722"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6250996.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16580..17185
                     /gene="cysC"
                     /locus_tag="OO470_001723"
     CDS             16580..17185
                     /gene="cysC"
                     /locus_tag="OO470_001723"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6250997.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17235..17558
                     /locus_tag="OO470_001724"
     CDS             17235..17558
                     /locus_tag="OO470_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6250998.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17752..18063
                     /gene="ftsB"
                     /locus_tag="OO470_001725"
     CDS             17752..18063
                     /gene="ftsB"
                     /locus_tag="OO470_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6250999.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18082..18792
                     /gene="ispD"
                     /locus_tag="OO470_001726"
     CDS             18082..18792
                     /gene="ispD"
                     /locus_tag="OO470_001726"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6251000.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18792..19271
                     /gene="ispF"
                     /locus_tag="OO470_001727"
     CDS             18792..19271
                     /gene="ispF"
                     /locus_tag="OO470_001727"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6251001.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19268..20317
                     /gene="truD"
                     /locus_tag="OO470_001728"
     CDS             19268..20317
                     /gene="truD"
                     /locus_tag="OO470_001728"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6251002.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20298..21059
                     /gene="surE"
                     /locus_tag="OO470_001729"
     CDS             20298..21059
                     /gene="surE"
                     /locus_tag="OO470_001729"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6251003.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21053..21679
                     /gene="pcm"
                     /locus_tag="OO470_001730"
     CDS             21053..21679
                     /gene="pcm"
                     /locus_tag="OO470_001730"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6251004.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21819..22958
                     /gene="nlpD"
                     /locus_tag="OO470_001731"
     CDS             21819..22958
                     /gene="nlpD"
                     /locus_tag="OO470_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6251005.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23021..24013
                     /gene="rpoS"
                     /locus_tag="OO470_001732"
     CDS             23021..24013
                     /gene="rpoS"
                     /locus_tag="OO470_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6251006.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24133..24540)
                     /locus_tag="OO470_001733"
     CDS             complement(24133..24540)
                     /locus_tag="OO470_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6251007.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24687..25280
                     /locus_tag="OO470_001734"
     CDS             24687..25280
                     /locus_tag="OO470_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6251008.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25280..26227
                     /locus_tag="OO470_001735"
                     /pseudo
     CDS             25280..26227
                     /locus_tag="OO470_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26500..27063)
                     /locus_tag="OO470_001736"
     CDS             complement(26500..27063)
                     /locus_tag="OO470_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251009.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27228..27479
                     /locus_tag="OO470_001737"
     CDS             27228..27479
                     /locus_tag="OO470_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6251010.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27481..>28174
                     /locus_tag="OO470_001738"
     CDS             27481..>28174
                     /locus_tag="OO470_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6251011.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVN"
BASE COUNT         6848 a         7009 c         7607 g         6710 t
ORIGIN      
        1 tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct cgtttgtatg
       61 acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt gtgctgatct
      121 ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt gcggaaatcg
      181 ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc tggtttaaca
      241 acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc aaaaccggca
      301 acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa atctacgaaa
      361 gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc atgctgggcg
      421 gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat gactacaacg
      481 ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc aacaaaatca
      541 tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc attggtaaac
      601 atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg gaagggctga
      661 aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg attgaaaaat
      721 aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc tcaggcgaga
      781 agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg gtgcgaatgg
      841 atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc ggcgcacacc
      901 tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt acgcccgcca
      961 tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt ggtttgtttc
     1021 ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa atcttcacct
     1081 tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc acgccagagc
     1141 atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac tcagtaggtc
     1201 acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg ggcgcattca
     1261 gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac tttatttccg
     1321 aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg ttgttgatta
     1381 cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag gggcgttttg
     1441 cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg ggcgatgaag
     1501 tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac tggcggcgca
     1561 cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg atttatacct
     1621 ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc agcctgatgc
     1681 ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac ctgctggcac
     1741 atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta gtaatggcct
     1801 gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc accaccattt
     1861 cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact gacattcgct
     1921 cgctgggcgt cggttttgcc accgccatga gtcgacttgg cgcggcggta agtactggcc
     1981 tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc ctagcgacag
     2041 tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa gccctcccgc
     2101 tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt taaagccgaa
     2161 ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg taatagcgga
     2221 ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc tgccgcgctg
     2281 ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag catgggggat
     2341 gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga agctgtattg
     2401 ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca tattgccgaa
     2461 tggcagcgtc agaatccgct ggatctgata attaccggct gccaaagcag cgaagggcag
     2521 aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt cacccaggtg
     2581 gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga acatgggttg
     2641 cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg tgaagtggcc
     2701 ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat tatccgtaaa
     2761 actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc agagcaacga
     2821 cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa gctatggcag
     2881 gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata aatcaggaga
     2941 atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg ctggcgcact
     3001 ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg gtggaacagt
     3061 ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc gatgaactgt
     3121 cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact tcgttagata
     3181 ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg acgctgcaaa
     3241 cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg aaaaacgcca
     3301 cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc gactggctgg
     3361 ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc agacgggtgc
     3421 tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg gcagaaaagc
     3481 tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt gatgctgaaa
     3541 aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt gttggcgctt
     3601 ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg gcgattaatc
     3661 atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat gactggaaag
     3721 tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt gctgccggat
     3781 gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg ttaagcgtcg
     3841 catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa attcaattgg
     3901 ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt attcgtggtg
     3961 gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc gcataccagc
     4021 ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac tttgggcatg
     4081 cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt atcaatgttg
     4141 tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac ttccgctctt
     4201 gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt caccagtttc
     4261 aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac atgaataaaa
     4321 gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca gatattgccg
     4381 tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct ggcattatct
     4441 ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat aaattatcct
     4501 tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc gccactgttg
     4561 tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc cgcgatagtc
     4621 aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg cgttaatcaa
     4681 ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg catcagcaac
     4741 gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc cgccagatgc
     4801 cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg aacaggttta
     4861 tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca cgcccgatac
     4921 cagccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac atcccatagc
     4981 aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc gcggtgctgg
     5041 caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat atccatgccg
     5101 cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag cagccagccg
     5161 ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag atgcgcacca
     5221 tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc tttaaagcga
     5281 gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg gcaaacaatc
     5341 cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa gtcatcacag
     5401 attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa acgttcttca
     5461 atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc ttcgccagca
     5521 ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc tgccagcacc
     5581 acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct gccgttttct
     5641 tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt ttcggcttcg
     5701 gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc aggattcacc gccgggctgt
     5761 aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc gtgagtgatg
     5821 cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag tgcatgggta
     5881 tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc gagtaacaaa
     5941 acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat accggcacca
     6001 ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga acacaacacc
     6061 ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat tcgccgcgat
     6121 agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc ggcacaacgg
     6181 gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt tccagacctg
     6241 gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac gccgctttca
     6301 gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta atttccagtc
     6361 gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg acatgtggta
     6421 agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc ataatttctc
     6481 tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca ttatctaaaa
     6541 cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc atttgttttt
     6601 cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg ttagtcgatt
     6661 tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt taaccttacc
     6721 ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta ctggaacata
     6781 acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc cggagcaact
     6841 ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct gggtttctgg
     6901 ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc cagcgcccgc
     6961 cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg cgcgccgggt
     7021 tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc tggtgaacgc
     7081 gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag tgacgtcaac
     7141 gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc tgttctccaa
     7201 aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg atagctctta
     7261 tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac tggggggtga
     7321 acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca gcgagtggcg
     7381 cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt cgcaatccgt
     7441 cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg cgccgctggt
     7501 ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag acgttcgcca
     7561 tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg cgctgggcgt
     7621 ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt ggctgacagg
     7681 cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc tacagtggca
     7741 cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc ttacccgcag
     7801 tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg ccgcgacgac
     7861 gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag cgctaattaa
     7921 tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg cggaagtcga
     7981 gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc gcgcccgtgc
     8041 cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag tccgcgtatt
     8101 tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg tgattatgat
     8161 tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg ccgctgacga
     8221 agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag acttcctgta
     8281 tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg atctcgcctg
     8341 gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac agggcgcgga
     8401 gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg ctaatcgcat
     8461 ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg gcatggacac
     8521 cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc agcgagatgt
     8581 ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact gacagacgcc
     8641 gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga tttaaacgac
     8701 ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca cggcatgtat
     8761 cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga gccgcgccac
     8821 gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca gtggcaggcg
     8881 atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac caaccgccag
     8941 acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca gatgctgcac
     9001 tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt actctgcacc
     9061 tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa gaagatttct
     9121 gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca ggaaaaagtc
     9181 gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa gttcaaaacc
     9241 acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat gaacttcgtg
     9301 gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg tgggctttct
     9361 atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg ctatctgccg
     9421 ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga ctggggtaac
     9481 cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg ggttgagacg
     9541 tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg cccgtatgaa
     9601 tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa ctggcacctg
     9661 acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct gaaaactggc
     9721 ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa ccagaatctg
     9781 atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc caaagagagc
     9841 gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt gtcattcccg
     9901 acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat cgacaacatc
     9961 gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg tgtaacaggc
    10021 tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcttggtggg taaagcaccg
    10081 ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc acggatgtat
    10141 aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat agggcgctgg
    10201 gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc gggcatcatt
    10261 cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg gtcttgtagg
    10321 cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat ctatgtccaa
    10381 actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg cgctggcaga
    10441 aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg cgctggataa
    10501 tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg tgagcctgca
    10561 tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg gctatctgtt
    10621 cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca acctgaaagt
    10681 gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac tgtgggaaca
    10741 gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga tgaaccgggc
    10801 gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac aatccggcag
    10861 ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc tgccgattat
    10921 cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga aatatcaccc
    10981 attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta aatgggaacc
    11041 cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg gattacacga
    11101 agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg gcgtttttat
    11161 tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta acaggtatag
    11221 aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc cagatggaca
    11281 aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag caattcggat
    11341 gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct tgtggcaatc
    11401 atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc gttatgtgag
    11461 ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga atcgaagaag
    11521 tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg tctcctgcac
    11581 ccttttttta aatttatatt ttttatgtga atatatagtc tctatttatt attatgaatt
    11641 tcaaatgaaa atcattaatt tattcataat gtaaatgttt tattggctga tatatatcat
    11701 agatagtttt taaaatatgc gtaaatatcc tttaagttta ctgaaggata aaaatattgt
    11761 gactttcttt gatttctggg gaaaaaaccg acgtggcgag aaagagggta tcgacgacta
    11821 tcaccttctt tgctggcatt tgctggatgt ggccgcaatg ggctatttaa tggttaaaag
    11881 aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca aggaacaggc
    11941 agctcaattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg cccgctcttt
    12001 tcaacaactt tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa aaaattacga
    12061 aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa gtgaatgtca
    12121 ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga tagaaatgtg
    12181 gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg aattggataa
    12241 ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca agccactgtt
    12301 tccgcgaata gagatcccca cattctggga tgatgacgag ggcgttgaac ttataaaaca
    12361 actttcctgg tatatctctg caaccgtcgt acttgcagac tggacagggt cgtcaacgcg
    12421 attttttcca cgcgtcgcac aggcaatgga tattgaagat tactggcaaa aggctttagt
    12481 tcaggctcaa aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc ctttcaccgg
    12541 aattaatacg ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa aggtgctgga
    12601 tctggatatc aatcagccag gaccacagtt gtttattctg gaagatgtga ccggcgcagg
    12661 taaaacagaa gcggcgctta tcctggcgca caggttgatg gctgcgggta acgctcaggg
    12721 gctgtttttt ggcctgccaa caatggcaac ggccaatgcc atgtacgatc gactggtcaa
    12781 aacctggctt gctttctatt cgccagaggt gttccccgct ccagcggaga taaaccggcg
    12841 caatacggtg cggttttccc ggaagtaaac cggacgcact ggatgcgatg atgggtatca
    12901 cttagaattc cccgcccctg cggtagaact cccagctccc attttcaaac ccatcaagac
    12961 gccttcgcca gctccttcac caacggtagc attatccgca taacgtcacg gcagcgacgt
    13021 tctattcttc caggaagtgc cttatcaata tgttgttgat tatccagtct tacgtcatgc
    13081 cagctatttc ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc atccttattt
    13141 ccaagattcc agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt gtcgaatatt
    13201 tctgcgtcat tacaacaccc agtgcctttc ggataatttt tattcaaacc cggattggtc
    13261 gttgcggcta ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt taatttcctt
    13321 actgcttcag gggtttttac accgctgttg aaatacaatt tatcgccaac aattaagtta
    13381 tcgagattaa tcaccagcag cgtatttttc ttttcggtgt cactcatccg cttgagtaaa
    13441 ttctcagcgc ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg aataccatac
    13501 tcggtaggcg tatttttcag gcgttctgcc agttccagca tgacacctaa acctgcggcg
    13561 ttatcatcta ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc agcatcgctc
    13621 agcggggcgt aagtatccag atgcgccata atgatgatct gctgcggcgc tttgccttca
    13681 tgagcggcaa tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt atcgcgggcg
    13741 gtataaatat accgactatt aaatgtccga atatcactgc gataacccat ttgctgaaac
    13801 tgttggcgaa tataatcggc agataacatt tctgctggag ttccggtcat gcgtcccgga
    13861 aagaaagtag caatatgtcg tgcctgggta ttagcaaaat cgccaggttt aggtgacgag
    13921 gcgtgtacgg ggagaataaa gcatacgccg agcgccaggg cagcggtacg gtggcgcaat
    13981 gcggaaaaca tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca tagtatgaaa
    14041 ctgccgctgc gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg gtgccttaag
    14101 cactttttga tattagcttt gccaaatcgt tattccgtta aggaactact cattctaatt
    14161 ggtaatttca ttcgttctct tacgctccct atagtcgaaa catctgatgg caagaaaata
    14221 gcggtattgc aaaggaacgg ttatggatca aaaacgactt actcacctgc ggcaactgga
    14281 ggcggaaagc atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc cggtgatgct
    14341 ctactctatc ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg cgttttatcc
    14401 aggtacgctg cctttcccgt tgctgcatgt cgataccggc tggaaattcc gcgagatgta
    14461 tgagttccgc gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc ataaaaaccc
    14521 ggaaggcgtg gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac ataccgatat
    14581 tatgaaaact gaaggcctga aacaggcgct gaacaaatac ggttttgatg ccgccttcgg
    14641 tggtgcgcgc cgtgacgaag agaaatcccg cgctaaagag cgaatctact ctttccgtga
    14701 ccgcttccat cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca actacaacgg
    14761 gcaaattaac aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga ccgagcagga
    14821 tatctggcaa tacatctggc tggaaaatat cgacattgtg ccgctgtatc tcgctgcgga
    14881 acgtccggtt ctggaacgcg acggtatgtt gatgatgatt gatgacaacc gtatcgacct
    14941 gcaaccgggc gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg gctgctggcc
    15001 gctgaccggt gcggtggagt caaatgcaca aacactgccg gaaatcatcg aagagatgct
    15061 ggtttccacc accagtgaac gtcaggggcg cgtgattgac cgcgaccagg cgggatccat
    15121 ggagctgaaa aaacgtcagg ggtattttta agatgaacac cgtacttgca caacaaatcg
    15181 ccaatgaagg cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc ctgctgcgtt
    15241 ttctgacctg tggtagcgtc gatgacggca aaagtaccct gattggtcgt ctgctgcacg
    15301 atacccgcca aatctacgaa gatcagctct catcgctgca taacgacagt aagcgtcacg
    15361 gcacccaggg cgaaaagctg gatctggctc tgctggtgga cggcctgcaa gctgagcgcg
    15421 aacagggcat cactattgat gtggcctacc gctatttctc taccgagaag cgtaaattta
    15481 ttatcgccga caccccaggg cacgagcagt acactcgcaa tatggcgact ggcgcatcga
    15541 catgtgaact ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat caaacccgtc
    15601 gtcacagttt tatctccaca ctgttgggga tcaaacatct ggtcgtggcg atcaacaaaa
    15661 tggatctggt ggattacagt gaagagacgt tcacccgtat tcgtgaagat tatctgacct
    15721 ttgccgggca gctgccggat aatctggata tccgctttgt gccgctctcc gcactggaag
    15781 gtgacaacgt ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg acactgctcg
    15841 aagtgctgga aaccgtggag atccagcgag tggtggatgc ccagccaatg cgcttcccgg
    15901 tgcagtacgt taaccgccca aatctcgatt ttcgtggcta cgccggaatg ctggcatccg
    15961 gtcgcgtgga agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa tcaaacgtcg
    16021 cgcggatcgt gacttttgat ggtgatcgcg aagaagtctt tgccggagaa gcgatcaccc
    16081 tggtgctgac ggatgagatc gatatcagcc gtggtgatct gctgctggcg gcagacgaag
    16141 cgttaccggc ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa cagccgcttt
    16201 ccccgggcca gagttatgac atcaaaattg ccggtaagaa gacgcgtgct cgtgttgatg
    16261 gcattcgtta tcaggttgat attaataacc ttacccaacg cgaagttgaa aacctgccgc
    16321 tgaacggcat cggcctggtg gatctcactt ttgacgagcc actggtgtta gatcgctatc
    16381 agcaaaatcc ggttaccggt gggctgattt ttatcgatcg cctgagcaat gtgaccgtgg
    16441 gtgccggtat ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct gaattcagtg
    16501 cattcgaact ggaattgaat gccctggttc gtcgccactt tccgcactgg ggcgcgcgcg
    16561 atttgctggg ggataaataa tggcgctgca tgacgaaaac gtcgtctggc atagtcatcc
    16621 ggtcactgtg caacaacgcg aactacacca cggtcatcgt ggtgtagtgc tgtggtttac
    16681 cggcctctcc gggtccggta aatcaacggt cgccggggcg ctggaggagg cgttacataa
    16741 actcagcgtc agtacgtatc tgctggatgg cgacaatgtt cgccacggat tatgcagcga
    16801 tctcggtttt agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg aagtggcgaa
    16861 tttgatggtt gaagccggac tggtggtgct gaccgcattt atctcgccac accgcgccga
    16921 acgccagatg gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt ttgtcgatac
    16981 gccgctggcg atttgcgaag cccgcgatcc aaaaggctta tataagaaag cgcgtgccgg
    17041 tgaactgcgc aactttacgg gaatagattc cgtttacgaa gcgcctgaat cggcagaaat
    17101 tcatctcaat ggtgaacaat tagtaacaaa tttggtacag caattattag atctgttgag
    17161 acagaacgat attatcagat cctgagacac caccgggttt ccatgcccgg ttcgtcaaag
    17221 taacaggatc aaatatgcgt aatagccata acattacact aacaaataac gacagcctta
    17281 ctgaggatga agaaactaca tggtcactgc ctggtgccgt ggtcggtttt atctcctggt
    17341 tatttgcgct ggcgatgccg atgttgattt atggctctaa cacgctgttc ttctttctct
    17401 acacctggcc tttctttctg gcactgatgc ctgtcgcggt agtggtgggg attgcgctgc
    17461 attcattgat ggacggaaag ctacgctaca gtattgtttt caccctggtg acagtaggca
    17521 ttatgtttgg ctcactgttt atgtggctac tgggctaatt tgtactttct tgtctcctct
    17581 gttcataatt caaaccgtaa ctaataatga gattatgttc tgcacgccct gggtatacgt
    17641 aacaatggac aaatgtggta catttgcccg cgttgtcgcg gtatccccaa cagaggatgt
    17701 agagtcgtct tcggatgcat gggatgatga tgcagttttt cagggggcag gatgggtaaa
    17761 ctaacgctgc tgttgctggc tattctggtc tggctacagt attcgctgtg gttcggtaag
    17821 aacggtatac atgactatac ccgcgtcaat gatgatgtgg cggcactgca agctacaaac
    17881 gcgaaactta aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct caatggcggc
    17941 caggaggcgc tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc gggcgaaact
    18001 ttttatcgtc tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca aaacaatcga
    18061 taaatcagcc cgggaattaa catggcaacc actcatttgg atgtttgcgc cgtggttccg
    18121 gcggccggat ttggccgtcg aatgcaaacg gaatgtccta agcaatatct ctcaatcggt
    18181 aatcaaacca ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg ggtgacacgt
    18241 gtcgtcattg ccataagtcc tggcgatagc cgttttgcac aacttcctct ggcgaatcat
    18301 ccgcaaatca ccgttgtaga tggcggtgat gagcgtgccg attccgtgct ggcaggtctg
    18361 aaagccgctg gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat
    18421 caggatgacc tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg agggatcctc
    18481 gccgcaccag tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat
    18541 accgttgatc gcaacggctt atggcacgcg ctgacgccgc aatttttccc tcgtgagctg
    18601 ttacatgact gtctgacgcg cgctctaaat gaaggcgcta ccattaccga cgaagcctcg
    18661 gcgctggaat attgcggatt tcatccgcag ttggtcgaag gccgcgcgga taacattaaa
    18721 gtcacgcgcc cggaagattt ggcactggcc gagttttacc tcacccgaac catccatcag
    18781 gagaatacat aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc ggtgaaggcc
    18841 caattatcat tggtggcgta cgcattcctt acgaaaaagg attgctggcg cattctgatg
    18901 gcgacgtggc gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg ctgggggata
    18961 tcggcaaact gttcccggat accgatccgg catttaaagg tgccgacagc cgcgagctgc
    19021 tacgcgaagc ctggcgtcgt attcaggcga agggttatac ccttggcaac gtcgatgtca
    19081 ctatcatcgc tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc gtgtttattg
    19141 ccgaagatct cggctgccat atggatgatg ttaacgtgaa agccactact acggaaaaac
    19201 ttggatttac cggacgtggg gaagggattg cctgtgaagc ggtggcgcta ctcattaagg
    19261 caacaaaatg attgagtttg ataatctcac ttacctccac ggtaaaccgc aaggcaccgg
    19321 gctgctgaaa gccaatccgg aagactttgt ggtggtggaa gatttgggct ttgagcctga
    19381 tggtgaaggt gagcatattc tggttagaat cctcaaaaac ggctgcaata cccgttttgt
    19441 ggcggatgca ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct tcgctgggca
    19501 aaaagacaaa catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg gcaaggaaat
    19561 gcccgatctg agcgcctttc aactggaagg ctgccaggtg ctggagtatg cgcggcacaa
    19621 gcgcaagctg cgtttagggg cgctgaaagg taacgccttt accctggttc tgcgcgaagt
    19681 gagcaatcgc gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag gtgtaccgaa
    19741 ctacttcggt gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg cgctgcgctg
    19801 ggcgcaaacc aatactccgg tgcgcgatcg caataaacgg agtttttggt tgtcggcagc
    19861 ccgcagtgcg ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag acgttaatca
    19921 agttgttgac ggcgatgcgc tacaattagc cggacgtggt agctggtttg tagcaaccac
    19981 cgaagaactg gcggaattac agcgtcgcgt caacgataaa gagctgatga taaccgccgc
    20041 attgccgggc agtggcgaat ggggaactca gcgtgaagcg ctggcattcg aacaagcagc
    20101 tgtcgccgca gaaactgaat tacaagcttt actggtgcgc gaaaaagttg aagccgcgcg
    20161 cagagcgatg ctgctgtatc cgcaacaatt aagctggaac tggtgggatg acgtcaccgt
    20221 agagatccgt ttctggcttc cggcgggtag ttttgcaacc agcgttgtca gggaacttat
    20281 caacacaaca ggtgattatg cgcatattgc tgagtaatga tgacggggta catgcacccg
    20341 gtatacaaac gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg gtcgcccccg
    20401 atcgtaaccg cagcggcgct tcaaattctc tgacactgga atcctccctg cgcacgttta
    20461 cctttgaaaa tggtgatatt gctgtgcaaa tgggaacccc gaccgattgt gtctatcttg
    20521 gcgtgaatgc tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt aacgccgggc
    20581 cgaatctggg ggatgatgtt atttattccg gtacggtagc cgccgcgatg gaaggccgtc
    20641 atttaggttt tccggcgctg gccgtctcgc ttgacgggca taaacattac gacactgccg
    20701 cggcggtaac ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc accggacgta
    20761 ttcttaatat taacgttcag gatttaccct tggatcaaat caaaggtatt cgcgtgacgc
    20821 gctgcggtac acgacatccg gcagatcagg tgatcccgca gcaagatccg cgcggcaata
    20881 cgctgtactg gattggcccg ccgggcggta aatgtgatgc cggtccgggg accgattttg
    20941 ctgcggtaga tgagggctat gtctccatca cgccgctgca tgtggattta actgcgcata
    21001 gcgcgcaaga tgtggtttca gactggttaa acagcgtggg agttggcacg caatggtaag
    21061 cagacgcgta caagcacttc tggatcaatt acgtgcgcaa ggtattcagg atgagcaggt
    21121 gctgaatgca cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt ttgaacaaaa
    21181 agcctgggac aatatcgcct tgccgatagg tcaggggcag acaatttcgc agccgtatat
    21241 ggtggcgcga atgaccgaat tactcgagct gacgccgcag tcgcgggtgc tggaaattgg
    21301 cactggttcg ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg tttgctcggt
    21361 tgaacggatt aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc ttgatttaca
    21421 taatgtttca acccgtcatg gtgatggatg gcaaggttgg caggcacgtg cgccgtttga
    21481 cgctatcatt gttacggcgg caccgccgga aattccaact gcgctaatga cgcagctgga
    21541 cgaaggcggg attctcgtct tacccgtagg ggaggagcac cagtatttga aacgggtgcg
    21601 tcgtcgggga ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg tccctttagt
    21661 gaagggtgag ctggcttaaa acgtgaggaa atacctggat ttttcctggt tattttgccg
    21721 taggtcagcg tatcgtgaac atcttttcca gtgttcagta gggtgccttg cacggtaatt
    21781 atgtcactgg ttattaacca atttttcctg ggggataaat gagcgcggga agcccaaaat
    21841 tcaccgttcg ccgcattgcg gctttgtcac tggtttcgct atggctggca ggctgttctg
    21901 acacttcaaa tccaccggca ccggtcagct ccgttaatgg caatgcgcct gcaaatacta
    21961 attctggtat gttgattacg ccgccgccga aaatggggac gatgtctaca gcgcagcaac
    22021 cgcaaattca gccggtacag cagccacaaa ttcaggctac tcaacaaccg caaatccagc
    22081 cagtgcagcc agtagctcag cagccggtac agatggaaaa cggacgcatc gtctataacc
    22141 gtcagtatgg gaacattccg aaaggcagtt atagcggcag tacctatacc gtgaaaaaag
    22201 gcgacacact tttctatatc gcctggatta ctggcaacga tttccgtgac cttgctcagc
    22261 gcaacaatat tcaggcacca tacgcgctga acgttggtca gaccttgcag gtgggtaatg
    22321 cttccggtac gccaatcact ggcggaaatg ccattaccca ggccgacgca gcagagcaag
    22381 gagttgtgat caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta
    22441 cgtattctga gtcttcgggt gaacagagtg ctaacaaaat gttgccgaac aacaagccaa
    22501 ctgcgaccac ggtcacagcg cctgtaacgg taccaacagc aagcacaacc gagccgactg
    22561 tcagcagtac atcaaccagt acgcctatct ccacctggcg ctggccgact gagggcaaag
    22621 tgatcgaaac ctttggcgct tctgaggggg gcaacaaggg gattgatatc gcaggcagca
    22681 aaggacaggc aattatcgcg accgcagatg gccgcgttgt ttatgctggt aacgcgctgc
    22741 gcggctacgg taatctgatt atcatcaaac ataatgatga ttacctgagt gcctacgccc
    22801 ataacgacac aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa aaaatagcaa
    22861 ccatgggtag caccggaacc agttcaacac gcttgcattt tgaaattcgt tacaagggga
    22921 aatccgtaaa cccgctgcgt tatttgccgc agcgataaat cggcggaacc aggcttttgc
    22981 ttgaatgttc cgtcaaggga tcacgggtag gagccacctt atgagtcaga atacgctgaa
    23041 agttcatgat ttaaatgaag atgcggaatt tgatgagaac ggagttgagg tttttgacga
    23101 aaaggcctta gtagaagagg aacccagtga taacgatttg gccgaagagg aactgttatc
    23161 gcagggagcc acacagcgtg tgttggacgc gactcagctt taccttggtg agattggtta
    23221 ttcaccactg ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga
    23281 tgtcgcctct cgccgccgga tgatcgagag taacttgcgt ctggtggtaa aaattgcccg
    23341 ccgttatggc aatcgtggtc tggcgttgct ggaccttatc gaagagggca acttggggct
    23401 gatccgcgcg gtagagaagt ttgacccgga acgtggtttc cgcttctcaa catacgcaac
    23461 ctggtggatt cgccagacga ttgaacgggc gattatgaac caaacccgta ctattcgttt
    23521 gccgattcac atcgtaaagg agctgaacgt ttacctgcga accgcacgtg agttgtccca
    23581 taagctggac catgaaccaa gtgcggaaga gatcgcagag caactggata agccagttga
    23641 tgacgtcagc cgtatgcttc gtcttaacga gcgcattacc tcggtagaca ccccgctggg
    23701 tggtgattcc gaaaaagcgt tgctggacat cctggccgat gaaaaagaga acggtccgga
    23761 agataccacg caagatgacg atatgaagca gagcatcgtc aaatggctgt tcgagctgaa
    23821 cgccaaacag cgtgaagtac tggcacgtcg attcggtttg ctggggtatg aagcggcaac
    23881 actggaagat gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc agattcaggt
    23941 tgaaggcctg cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct
    24001 gttccgcaag taagtaagca tctgtcagaa aggccagtct caagcgaggc tggccttttt
    24061 cttttgtttg ggaagcttgt gtttttgtcg cctggataag acacgtcagc gtcctacccg
    24121 gcaatcagca ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca gctcctgctc
    24181 ttctccactc aaacgcccca aaaattcctc atccacgctg tcaccaatag gtatcgccgc
    24241 agcaagtagc ttttctcctt cggcagtcag ccagacaaag cgtcgccgct tgtcagcgac
    24301 gtcatgttca cgtctgacta agccacgatt ttccattctt gccaacattt ctgctagcgt
    24361 tgctttggtg ctgactgctg cttctatcag tgccacctgt tcaataccag gtttatcagc
    24421 aatggcacgc atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt gctgccagcg
    24481 agccgtgtgc tgctgaaaaa gctgtcgtaa ctgatggaac gctttatttc gtaacgccat
    24541 gtaaactccg ggctaattct ctgtcgctat catagcggtt ttttagtgac gaagaagatg
    24601 ttgttacttt tcaatccttg ccgctgtgga aatgaaggag tatgttaata ataacgttcg
    24661 tatacgaata attaagagaa taagcaatga aactgatcgt cgggatgaca ggggctaccg
    24721 gtgcgcctct tggtgtggca ttactgcaag cgctgcgtga gatgccgaat gtcgagactc
    24781 atctggtgat gtcgaagtgg gcgaaaacca ccattgaact ggaaacgcct tacagcgctc
    24841 gcgatgttgc tgccctcgca gacttcagcc ataacccggc ggatcaggcg gcgatcatct
    24901 catccggttc ttttcgtacc gacggcatga tcgttattcc gtgcagtatg aaaacgctcg
    24961 ccggtatccg cgctggttac gccgatggcc tggtagggcg cgcggcggac gtcgtgctca
    25021 aagaaggccg caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc atccatctcg
    25081 aaaatatgct cgcactttca cgcatgggcg tggcgatggt gccgccgatg cctgcctttt
    25141 ataaccatcc cgaaacggta gatgacattg tccaccatgt ggtagcccgc gtgctggatc
    25201 aatttggcct cgaacatccc cacgccaggc gctggcaagg attaccgcag gcccggaatt
    25261 tttctcagga gaatgaataa tggcatttga tgatttacgc agctttttac aggcgcttga
    25321 tgaccacggc cagttactga aaatcagcga agaagtgaac gctgagccgg atctggcagc
    25381 agcagctaac gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt ttgataatat
    25441 tcgcggcttt accgatgccc gcgtggcgat gaataccatc ggttcctggc agaaccacgc
    25501 gatttccctt ggcctgccac caaacacccc ggtcaaaaag cagattgatg agtttatccg
    25561 ccgctgggat aacttcccga ttgccccgga gcgccgcgcc aatccagcct gggcgcagaa
    25621 caccgtcgat ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc gcctgaatga
    25681 tggcgatggc ggtttctatc tcgacaaagc gtgcgtggtt tcccgcgatc cgctcgaccc
    25741 ggataacttc ggcaagcaga acgtcggcat ctaccgcatg gaagtgaagg gcaagcgtaa
    25801 gctcggcctg caaccggtgc cgatgcacga tatcgccctg catctgcata aagcagaaga
    25861 gcgcggtgaa gatctgccga ttgcgatcac gctcggtaac gatccgatca tcacgctgat
    25921 gggggccacg ccgctgaaat acgatcagtc tgagtatgaa atggcaggcg cgctgcgtga
    25981 aagcccgtac ccgatcgcca ccgctccgct gaccggtttt gatgtgccgt ggggttcaga
    26041 agtgatcctc gaaggggtta tcgaaagccg taaacgcgaa atcgaagggc cgttcggtga
    26101 gtttaccggg cactactccg gtgggcgcaa catgaccgtg gtgcgcatcg ataaagtctc
    26161 ttatcgcagc aaaccgattt tcgaatcact ctatctcggt atgccgtgga ccgaaatcga
    26221 ctacctatga ctaggtaatt tattcaagat aaccgggaag agattaaaaa agacgggaat
    26281 agattggaat caacaagtta ggaactcaag gttttcaaga taaagcgagt ccaaaaatgt
    26341 taaacggttc gatatcaata aaacattcaa gataactcac aggttcttac aatagccttt
    26401 aaagaccgtt caaaaacgat tcaggtgttt attatagcgc tgatgggtgc aagcgccaaa
    26461 accaatgtaa ttttagcaca gctaggcact tgcactgggt tatgcgctgg ctgctttctg
    26521 aggtagattt aagtgatcgc ccttagcttc ggacaacgcg taaatctctc taaccttttc
    26581 gtctggcaaa ttaccaaggc ttaacgctat ggctctacga actccaacta gagcgtgcaa
    26641 ctcttggatg tgctcatcca gaagaatgag taattgctcc gctccttttc tcccaagaac
    26701 atcagctact tgttgttttt cttgtggttt aagaaatgac aagaacatta gaatttgagc
    26761 atcaacatta tccgcattct cttttttggg ttctttggaa tcttttagtt caacatcacc
    26821 ctctcccgtc atgagccatt ccaaggtaac attttcgctt tctgctatct tttttaactg
    26881 atgtgggcgt ggtatcggtg cgtaatcccg cctgttgtaa taatttttta aagtgccttc
    26941 attgatcccc catgcccgag cagccgcatt tcttgaaggg tatcgttcta ctaacctaaa
    27001 gattctttcg ataatcggtt cttttccatc ggcaggaaaa gaaccttggg gtgcttttct
    27061 cattcttggt tcctttctat ctaattcatt gataaataat aaaattaaaa aataaaccat
    27121 aaaaacacca aagaaccctt tacagggaat ctttggtatc ttaatcttta tccatacgga
    27181 tatccgaccg gatatccgcg cgggtaattt tgtaggttaa aggaaagatg agacaaagag
    27241 aagatagtga ccgcgattgg ccttcgctga agattcagct ggcgctcaaa gataacgggt
    27301 ggtcttcctt ccgggagctt tcactccatg ctggttatgc tcaggattca ctgaaagcgg
    27361 tagttcgcac accaaacaaa aaatacgaac gcgtaatttc tgacgctata ggtgtaccgc
    27421 cagaaatcat ctggcccagc cggtatcgta acgatggatt tatcaggaag gcgtcgtgat
    27481 atgcagtcgt acgttaccgt aaatgacctg ctcggcgtac caggaatgcc agccaccaca
    27541 aaaggcatcc gacaggcgtt acagcgtttt tctcgtgatc ttggtgacgt tagtcgccgt
    27601 cgggaaggca caaaagccat tgagtaccac attgattgtc tgcctgaaat aacacgaaag
    27661 gcactgcgtg aacgctatgt ggaacagctg gtggctacag agaacaatgt ttctgaagtg
    27721 aaagctgtta ccagaaaaac acgcaatcct gacgctgtcc aggcaatcga agcatatcgc
    27781 ggttctccac aactgatgga agaacgcctg aatgcgctga ccgaaaacca gcgctgggta
    27841 tctgaagcaa gagctgcgct ggtggtggaa gtgctgaagc tggaaagcgc cggtaacccc
    27901 gggcgactga aagccattaa ctttcttgtt gaaaaagccc gtaaaggtga gctgccggag
    27961 cgcctgcaac aggccgcagt taacgccaat gcaaaacgtg gcgctaatcg tactatcagc
    28021 cgcgatccac tttaccagtg ggttctgaaa tacaaccagt cgcagaacgc cgccgaacgg
    28081 ctgctgttac tggcaccggg aaaacgcgac gaaataaaac cggaagaaat cagctggctg
    28141 cctgaatttc tggcgcagta tcgccaggtg aacg
//
LOCUS       DAJTUN010000042        27787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTUN010000042 DAJTUN010000000
VERSION     DAJTUN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.042"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..545
                     /gene="eutR"
                     /locus_tag="OO470_001739"
     CDS             <1..545
                     /gene="eutR"
                     /locus_tag="OO470_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6251012.1"
                     /translation="ALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLL
                     MAMGAMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRT
                     LQNAFHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDY
                     QQLFAEKQSLTLHQRMREWG"
     gene            complement(551..1450)
                     /gene="hemF"
                     /locus_tag="OO470_001740"
     CDS             complement(551..1450)
                     /gene="hemF"
                     /locus_tag="OO470_001740"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6251013.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(1454..2323)
                     /gene="amiA"
                     /locus_tag="OO470_001741"
     CDS             complement(1454..2323)
                     /gene="amiA"
                     /locus_tag="OO470_001741"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6251014.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            2537..2962
                     /locus_tag="OO470_001742"
     CDS             2537..2962
                     /locus_tag="OO470_001742"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6251015.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            2949..3398
                     /locus_tag="OO470_001743"
     CDS             2949..3398
                     /locus_tag="OO470_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6251016.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            3459..4034
                     /locus_tag="OO470_001744"
     CDS             3459..4034
                     /locus_tag="OO470_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6251017.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            4130..5029
                     /gene="yfeX"
                     /locus_tag="OO470_001745"
     CDS             4130..5029
                     /gene="yfeX"
                     /locus_tag="OO470_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6251018.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(5209..6633)
                     /gene="murP"
                     /locus_tag="OO470_001746"
     CDS             complement(5209..6633)
                     /gene="murP"
                     /locus_tag="OO470_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6251019.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(6637..7533)
                     /gene="murQ"
                     /locus_tag="OO470_001747"
     CDS             complement(6637..7533)
                     /gene="murQ"
                     /locus_tag="OO470_001747"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6251020.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            7697..8497
                     /gene="murR"
                     /locus_tag="OO470_001748"
     CDS             7697..8497
                     /gene="murR"
                     /locus_tag="OO470_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6251021.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            8684..9475
                     /gene="ucpA"
                     /locus_tag="OO470_001749"
     CDS             8684..9475
                     /gene="ucpA"
                     /locus_tag="OO470_001749"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6251022.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            9779..10795
                     /gene="cysP"
                     /locus_tag="OO470_001750"
     CDS             9779..10795
                     /gene="cysP"
                     /locus_tag="OO470_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6251023.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            10795..11628
                     /gene="cysT"
                     /locus_tag="OO470_001751"
     CDS             10795..11628
                     /gene="cysT"
                     /locus_tag="OO470_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6251024.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            11628..12503
                     /gene="cysW"
                     /locus_tag="OO470_001752"
     CDS             11628..12503
                     /gene="cysW"
                     /locus_tag="OO470_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6251025.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            12493..13590
                     /gene="cysA"
                     /locus_tag="OO470_001753"
     CDS             12493..13590
                     /gene="cysA"
                     /locus_tag="OO470_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6251026.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            13724..14635
                     /gene="cysM"
                     /locus_tag="OO470_001754"
     CDS             13724..14635
                     /gene="cysM"
                     /locus_tag="OO470_001754"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6251027.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14839..15562)
                     /locus_tag="OO470_001755"
                     /pseudo
     CDS             complement(14839..15562)
                     /locus_tag="OO470_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(15595..15969)
                     /locus_tag="OO470_001756"
     CDS             complement(15595..15969)
                     /locus_tag="OO470_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6251028.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            16074..16925
                     /gene="pdxK"
                     /locus_tag="OO470_001757"
                     /gene_synonym="thiJ"
     CDS             16074..16925
                     /gene="pdxK"
                     /locus_tag="OO470_001757"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6251029.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(16967..17476)
                     /gene="crr"
                     /locus_tag="OO470_001758"
     CDS             complement(16967..17476)
                     /gene="crr"
                     /locus_tag="OO470_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6251030.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(17517..19244)
                     /gene="ptsI"
                     /locus_tag="OO470_001759"
     CDS             complement(17517..19244)
                     /gene="ptsI"
                     /locus_tag="OO470_001759"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6251031.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(19289..19546)
                     /gene="ptsH"
                     /locus_tag="OO470_001760"
     CDS             complement(19289..19546)
                     /gene="ptsH"
                     /locus_tag="OO470_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6251032.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(19930..20901)
                     /gene="cysK"
                     /locus_tag="OO470_001761"
     CDS             complement(19930..20901)
                     /gene="cysK"
                     /locus_tag="OO470_001761"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6251033.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(21086..21847)
                     /gene="cysZ"
                     /locus_tag="OO470_001762"
     CDS             complement(21086..21847)
                     /gene="cysZ"
                     /locus_tag="OO470_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6251034.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            22077..23063
                     /gene="zipA"
                     /locus_tag="OO470_001763"
     CDS             22077..23063
                     /gene="zipA"
                     /locus_tag="OO470_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6251035.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            23134..25149
                     /gene="ligA"
                     /locus_tag="OO470_001764"
     CDS             23134..25149
                     /gene="ligA"
                     /locus_tag="OO470_001764"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6251036.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            25151..25369
                     /locus_tag="OO470_001765"
     CDS             25151..25369
                     /locus_tag="OO470_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6251037.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(25366..26364)
                     /locus_tag="OO470_001766"
     CDS             complement(25366..26364)
                     /locus_tag="OO470_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6251038.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            26454..27380
                     /locus_tag="OO470_001767"
     CDS             26454..27380
                     /locus_tag="OO470_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6251039.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(27371..27712)
                     /locus_tag="OO470_001768"
     CDS             complement(27371..27712)
                     /locus_tag="OO470_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6251040.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
BASE COUNT         6699 a         6897 c         7489 g         6702 t
ORIGIN      
        1 cggcgctgga agtgaaagag cagcataaag ccgcgctgtg gggctttgtc caacaggcgc
       61 tggcgacgtt ttgcgagaac ccggaaaatc tccatcagcc tgcagtgcga aaagtgctgg
      121 gggataattt gctaatggcg atgggggcga tgctggaaga agcgcaacca atggtgacgg
      181 cggaaagcat cagtcatcag agttaccgtc ggttgctttc ccgcgcccgt gaatatgtgc
      241 tggaaaacat gtccgaaccg gtgacggtgc tggatttgtg taatcaactg catgtcagtc
      301 gccgcacgct acaaaacgcg tttcacgcta ttttaggcat tggcccaaac gcgtggctga
      361 aacgcattcg cctgaacgcc gtacgccgcg aactgataag cccgtggtcg caaagcacaa
      421 cggtaaaaga cgccgccatg cagtggggat tctggcatct ggggcaattt gccacggatt
      481 accagcagct gtttgccgag aagcagtcgt tgacgttgca tcagcggatg cgggaatggg
      541 ggtgagggag ttacacccaa tccctgacct taataaactc acttaacgcc gcttctgggc
      601 tgccatcttt tggctgataa tcatactccc agcgtaccag tggcggcatt gacatcagga
      661 tcgactcggt gcgcccgcca gtttgcaggc caaacagcgt gccgcgatcc cagaccagat
      721 tgaactcgac ataacgaccg cgacggtaga gctgaaaatt gcgctcgcgc tcgccgtagg
      781 ccatcgcttt acgtcgctct acaattggta aataagcgtc ggtgtagcct ttgcctaccg
      841 cctgcataaa ggcaaaacag tggtcgaaat ctggcgtgtt cagatcatca aagaacagcc
      901 cgccaatgcc gcgctgttcg ttgcgatgtt tgaggtagaa gtaatcgtcg caccactttt
      961 tgtaacgggg ataaacgtct tcaccaaatg gctggcacag gtcacgggcg gtgcggtgcc
     1021 agtgaatggc gtcttcttca aaaccataga aaggggttaa atcgaagccg ccgccaaacc
     1081 accaaacggg ctctgcaccc ggtttttcgg caataaaaaa acgcacattc gcgtggctgg
     1141 tgggaacata cgggttatgc ggatgcacta ccagtgaaac gcccatcgcc tcgaaactgc
     1201 gcccggcaag ttccgggcga tgagcggtgg cggaagcagg catcgcctca ccgtggacgt
     1261 gagaaaagtt gacgcccgcc tgttcgaaaa caccaccatt acgcaacacc cgactgcgcc
     1321 cgccgccgcc agcttcgcgc tgccaactat cttcgacaaa ttctgcgcca tcgacggcgg
     1381 tcagctgctg acaaatcgta tcctgaaggt tgagcagaaa ctgtttaacc tggtgtgcgt
     1441 cgggtttcat aacttatcgc tttttcgaat gtgctttctg gttgtcgaac cagtggaaat
     1501 aactgatcac gccttcagca atcgctgtgg cgattttctg acgaaacgcc gccgtgccta
     1561 acagccgctc ttcttccggg ttggtgataa acgaggtttc caccagcacc gaaggaaccg
     1621 acggcgattt caacaccaca aacgcggctt gttcggtgtt gcggctgtgc agtttatgca
     1681 ccggcttaat ctttttcaga atatgcgagc caagcgtcag gctgttttta atagtatcgg
     1741 tttgcaccag atcaaacagt acttgctgca ataggtgatc cttgtcagtc gcctttttac
     1801 cggcaacttc atcggcgcgg ttttcgcgtt cagacaggta tttcgccatt gcgctacttg
     1861 ccccacggtt agagagggca aataccgaag caccggcagc tttcgggttg gtaaagccat
     1921 cggcatgaat tgacataaac agatctgcgc catgtttatg ggcgatttca acgcgatcgt
     1981 aaagtgggat aaacgtatcg ccagaacgcg ttaaacgcgc atcaatccca tgattacgca
     2041 aaatggaacg gacgttttta gcaatcgcca gcaccacatg tttttctttc gaaccgttgc
     2101 gtccgatcgc tccggtatca attccgccgt ggcctggatc gagaacaacg acacgtttgc
     2161 cgccagattt tttggctttc ggcttgctgt gtccgttgct ggtttttaaa ggttcgtctt
     2221 tggcaatggc ttgcgacatt cctgacaacg tcagggcagc taaaccggct ttcagcacct
     2281 ggcggcgcga agtgagtgtt tttagtggtt taaaagtgct catacggcct gagttgtaat
     2341 aataaagttc cagatgttat atcgtatcgc gtaatccgtt acgacggttt gctgttgaga
     2401 attgttttac ttttcatttc aatagttgac aactccccat tatgccactt tttcaaccga
     2461 ttttcgtcag atattggcta attcgaccgt tcacgccata atcagccttt taacccgcaa
     2521 aaaggtaacg aataccatgg agatacgcgt atttcgccag gaagatttcg aagaggtcat
     2581 caccctttgg gagcgttgcg acttgctgcg tccgtggaac gatccggaaa tggacatcga
     2641 gcgtaagatg aaccatgacg tcagtttgtt tttggtcgct gaggtaaacg gtgaagtggt
     2701 cggaacggtg atgggcggtt atgacgggca tcgcgggtct gcttattatc ttggcgtgca
     2761 tccagagttt cgtgggcgtg gaattgccaa tgcgttgctt aaccggctgg agaaaaagct
     2821 gattgctcgc ggctgcccga aaattcagat caacgtgccg gaagataacg acatggtgct
     2881 cggaatgtat gaacgcctgg gatatgaaca cgccgacgtg ctgagtctgg gtaagcgttt
     2941 gattgaagat gaagagtact gagttccatc ctgtccatta tgatgcgcac ggacgcctgc
     3001 gtttaccctt gctcttctgg cttgtgctat tgcttcaggc gcgaacctgg gtgctgtttg
     3061 tcattgccgg tgcgtcgcgt gagcagggta ctgcgctgct aaatctgttt tatcccgatc
     3121 acgataattt ctggctgggg ctgattcccg gtattccggc ggtgctggcg tttttgttga
     3181 gcggtcggcg ggcttcgttt cctcgcacct ggcatgtgct ctattttctg ttgctgttgg
     3241 cgcaggtggt tttactttgc tggcaaccgt ggctgtggct gaacggtgaa tccgttagcg
     3301 gtatcggtct ggcgctggta gtggcggata ttgtggcgtt aatctggctg ctgaccaatc
     3361 gacgtttacg cgcttgtttt aatgaagaaa aagaataacg gcactttttg gtgaatttgc
     3421 actccaagca acgttattga ataaccaaag gaagtgacat gaaatcgctg cgtttaatgt
     3481 tatgcgctat gccgttgatg ctgaccggct gttccacgat gtcgtcagtt aactggtctg
     3541 ccgctaaccc gtggaactgg tttggatcgt ccaccaaagt gagcgagcag ggcgtgggtg
     3601 aattaacggc gtccacacca ctgcaagaac aagccattgc tgatgcgctt gatggcgatt
     3661 atcgcctgcg cagcggaatg aaaaccgcga acggcaacgt ggtgcgcttt tttgaagtga
     3721 tgaaaggcga caacgtggcg atggtgatta acggcgatca aggcacgatc agtcgcattg
     3781 acgtgctgga tagcgatatt cctgctgaca ccggtgttaa aatcggtaca ccgtttagcg
     3841 acctttacag caaagcattt ggcaattgcc aaaaagctga tggtgatgat aatcgtgccg
     3901 tcgaatgtaa agccgaaggc agtcaacata ttagctacca gttcagcggg gaatggcgtg
     3961 gtcctgaagg gttaatgcct tcggacgata ccctgaaaaa ctggaaagtc agtaaaatta
     4021 tctggcggcg ttaatttgcg tctgaacaaa ccgccgtcgc gaaaaaacac gtaaaatagc
     4081 gctcaacaat gccacggatt gcgtggcatt cttattttca ggaggaacaa tgtctcaggt
     4141 tcagagtggc attttgccag aacattgccg cgcggcgatt tggatcgaag ccaacgtgaa
     4201 aggggaagtt gacgccctgc gtgcggccag taaaacattt gccgacaaac tggcaacttt
     4261 tgaagcgaaa ttcccggacg cgcatcttgg tgcggtggtt gcctttggta acaacacctg
     4321 gcgcgctctg agcggcggcg ttggggcaga agagctgaaa gattttccgg gctacggtaa
     4381 aggccttgcg ccgacgaccc agttcgatgt gttgatccac attctttctc tgcgtcacga
     4441 cgtaaacttc tctgtcgctc aggcggcgat ggaagccttt ggtgactgca ttgaagtgaa
     4501 agaagagatc cacggcttcc gttgggtgga agagcgtgac ctgagcggct ttgttgacgg
     4561 tacagaaaac ccggcgggtg aagagacgcg ccgcgaagtg gcggttatca aagacggcgt
     4621 ggattccggc ggcagctatg tgtttgtgca gcgttgggag cacaacctga agcagctcaa
     4681 ccggatgagc gttcacgatc aggagatgat gatcgggcgc accaaagagg ccaacgaaga
     4741 gattgacggc gacgaacgtc cggaaacctc tcacctcacc cgcgttgatc tgaaagaaga
     4801 tggcaaaggg ctgaagattg ttcgccagag cctgccgtac ggcaccgcca gtggcactca
     4861 cggtctgtac ttctgcgcct actgcgcgcg tctgcataac attgagcagc aactgctgag
     4921 catgtttggc gataccgatg gcaaacgtga tgcgatgttg cgtttcacca aaccggtaac
     4981 cggcggctat tatttcgcac cgtcgctgga caagttgatg gcgctgtaag cgtttctgat
     5041 tcattgatta aaggccaggc agtaacatta ctgactggcc tttttattac tgctttaacg
     5101 ccgcatatac ttgatcaact accttctgtc agaacagggt attttgcggc gctgacgcta
     5161 caactaacag ccagcagaga aagatagagc attgtccgtt tcataatatc agtccagatt
     5221 gacgttacgg cagccaaaga gcgtggtgaa aataaacccg caaacccatg ccaccagaat
     5281 cccgccagca taaaccgcca ttgccggaag gatgccttgt gcggaagtca tcagcggcag
     5341 cgccaccaga ccagacggcc caaaggcgct gtttaagccc atcggcagac cccaccaggc
     5401 tatcaatccg ataaacaaac cgcccgccgc gccgcctaaa caggcggtaa caaacggctt
     5461 catgcggggc agggtgacac cgtaaatcag cggttcacca acgcccagca ggccgggaat
     5521 aatcgccccg cgtacctgac tgcgtagcgc actgtgcggt tgcgcccgcc agtagagtgc
     5581 cagcgccgcg cccacctggc ccgcgcctgc cattgaaagg atgggaaata agctgttgaa
     5641 tccctggctg tccattaacg cgaggtaaac aggaataaag ccctgatgca cgccaaatac
     5701 cacggcgatc aggaacagcc cggctaaaac cgcacaaccg aacggattac tgttcaggtg
     5761 cataaacagc catgacatac cttcgaataa ccagccaccc agcggcataa tgatcaggta
     5821 cgcaagcgta gcggtgatca gcagggtgat taacgaggtc agcagcatgt cgagatcgtc
     5881 cggcataaag cggcgcacca tgccttcaat gcgggcgcag gcccacgcgg caatcagcac
     5941 gccgataata ttgccgcgcg gatcgatggg cagaccaaag aaatcgtgaa aaccggcgta
     6001 gtaaccggtg gttgcagcgg ggttataacc gagcaaaaag agtgcggcaa taattgcgcc
     6061 attgacgccc gtgccgccaa atgcctgggc ggcgttatag cccaccagaa tcactaggaa
     6121 ggtgaacaaa cctttgctga acaccttcat aaaattcagc gcgtcgggta gcgtgccctg
     6181 agcatctgcc ggaacgtgca tcaccgtggc aattaacgtc gctatcccca gcagcagacc
     6241 ggcggcaata aaaccgggga tcagcggcgt gaagatggtg gcgaatttgg cgagaaattg
     6301 ttgcacgccg gaggtttgtt tggcttttaa ctgacgtttg ttctgggcgg cgatttctgc
     6361 ggcatcctgt accggtgcat cgcccagcag ctcgctcatc gcttttgcgg cacgatgcgc
     6421 tttaccaggc ccaaaaacga cctgcacctg gtcgctggtc aaaatgacgc ccttcacgcc
     6481 ttccagcgtt ttgatattgg gatcaaccag tgaactgtca tgtacaccca gacgcaggcg
     6541 cgtcatacag ttaccacaac tggcgatatt tcccggtccg ccgacacggg taagaatggt
     6601 gttcagaagt tcactgctga tctctttggc catgggttat tccttgtcta aaacctgacg
     6661 aataaagccg ccgtgttgat ccaggcgttt tttagcttct gcggcatcga gatttttcag
     6721 caccatcaca atggccgttt tacagttgcg ctcgcaggca attaacgccg cttccgcttg
     6781 ttcggcatta catccggtgg cgtttttaac gatattgacc tggcgcacat gcagtttttc
     6841 gttggtggcg accacatcga ccatcaggtt gccgaacact ttgccggatt taatcatcag
     6901 cccggtggaa agcatattga gcaccagttt ctgcgctgta cctgctttca tccgcgaaga
     6961 accggtaaca acttcggcac ctacaatcgg tgtaatggca aactcagcgg tggttgaaac
     7021 ggcgctcccc ggattacagg aaattcccac tgtgcggcag ccgagctggc gtgcgtattc
     7081 cagtccggca atcacatacg gcgtgcgacc gctggcagca atgccaacca ccacatcctg
     7141 tgccgttaaa ttaatatttt tcagatcgtt aacaccacct tcccggctat cttcagctcc
     7201 ttccaccgcg tgctgaatgg catattcgcc tccagcaatc aaaccaacca ccagacccgg
     7261 tttcacgccg taggtgggcg gacattcgct ggcatccaga atccccagac gaccggatgt
     7321 tcccgcaccg agataaatca gacgaccgcc gccgctgacc tgggcgtgga taacatcgat
     7381 cgccgcggcg atatccggca ggacgcgatc aacggcaggc ggtacggttt tatcttcatc
     7441 gttgataatc cggcacattt ccagcgtcga tacgcggtca atttcagccg aggcggtgtt
     7501 cgagccttca gtaatcatct tttctagttg catcggtggt gaccttacta tttacgatca
     7561 aataattgca gatgtatgga atcatatatc ccgaattgac agacttaaag gaatcattga
     7621 gtctacactc gcagagtgtg aagagcgtca taagaactta agcggaattt ttgctgccag
     7681 cccaggagag tggaagatgt tgtacctgac gaaaatacgc aatgcggaaa gtgaatttac
     7741 cgggaacgag caaaaaattg ctgactttct gcgggccaga gtgagcgagc tgaaatccgt
     7801 ctcttcacgg cagatggcga aacagctcgg tatcagccag tcgagcattg tgaaatttgc
     7861 ccagaagctc ggcgcgcaag gatttactga attgcgtatg gcgttaattg gcgaatacag
     7921 tgccagccgg gagaaaacga atgccacggc gctgcacctg cacagttcga ttaccagtga
     7981 tgattcgctg gaagtgattg cccgcaaact gaatcgcgaa aaagagctgg cgctggagca
     8041 aacctgtgcg ttgttcgact acgcgcggct gcaaaaaatc attgaggtca tcagtaaagc
     8101 accgtttatc cagataacgg gcctgggcgg atcggcgctg gtggggcgcg atctgtcgtt
     8161 caaactgatg aaaattggct atcgcgtggc ctgcgaagcc gacacgcacg tacaagccac
     8221 cgtttcccag gcgttgaaaa aaggcgatgt acaaatcgct atctcttaca gcggcagtaa
     8281 aaaagagatt gtcttgtgcg cagaagcggc acgaaagcag ggggcaacgg tgattgccat
     8341 tacttctctg gctgactctc cgctgcggcg gctggcgcat ttcacgctcg ataccgtttc
     8401 cggtgaaacc gaatggcgta gctcgtcgat gtctacccgc acggcgcaaa attctgttac
     8461 cgatttactg tttgttggtc ttgtgcaaca tcaatgatgt ggcgtcattg aaaatgattc
     8521 agcgcagcag tgagctaaca caacgcctga aatgatgcat aaagcagcga ctggattgag
     8581 attttcctga attagtgagc tgatccgcag caatattttg tttatcctgt attttcagag
     8641 ggaatggagt gtaacgctct gtattaacaa ggagagcatt aaaatgggta aactcacggg
     8701 caagacagca ctgattacgg gcgcattgca gggaattggc gaaggaattg ccagaacttt
     8761 tgcacgtcat ggcgcgaacc taatcttgct ggatatctcc cctgagatcg aaaagctagc
     8821 ggacgaactg tgtggtcgtg gtcatcgctg tacggcggtt gtcgccgatg tgcgtgaccc
     8881 ggcgtcggta gccgcagcta tcaaacgcgc gaaggaaaaa gaagggcgca ttgatatcct
     8941 ggtgaataac gcaggcgttt gtcgtctggg cagtttcctc gatatgagcg atgaagatcg
     9001 cgatttccat attgatatca atattaaagg cgtatggaac gtcacgaagg cggtgctgcc
     9061 ggagatgatt gcgcgcaaag atggtcgcat tgtgatgatg tcttcagtca ctggtgatat
     9121 ggtggccgat cctggcgaaa cggcgtatgc cttaacgaaa gcggcgattg ttggcctgac
     9181 taaatcgctg gcggtggagt acgcgcaatc cggtattcgc gttaacgcca tctgcccggg
     9241 atacgtccgc acgccaatgg cggaaagcat tgcccgccag tcgaacccgg aagatccaga
     9301 atcggtgctg actgaaatgg caaaagcaat cccgctgtgt cgcctcgccg atccgctgga
     9361 agtcggcgaa ctggcggcct tcctcgcatc ggatgaatcc agctatttaa ccggtacaca
     9421 gaatgtgatt gatggcggca gcacactgcc ggagacggtt agcgtcggta tctgattcac
     9481 ctctgtttcc tccctgcatg tgtggggagg atttcgtctt gaactaagtt caccaggcta
     9541 tttcatttgt cattttggcc ccgggcagtg ctcgaaatcc tcacgtacta tgtgtacgct
     9601 ccggtttctc cgcgctgtcc gtgtccaaac tgcctgcaac aattacgcct gttgaaccaa
     9661 gttcttattc cctttttaac ttccaaatca ccaaacggta tataaaaccg ttactccttt
     9721 cacgtccgtt ataaatatga tggctattag aaagtcatta aatttataag ggtgcgcaat
     9781 ggccgttaac ttactgaaaa agaactcact cgcgctggtc gcttctctgc tgctggcggg
     9841 ccatgtacag gcaacggaac tgctgaacag ttcttatgac gtctcccgcg agctgtttgc
     9901 cgccctgaat ccgccgtttg aacagcaatg ggcaaaagat aacggcggcg acaaactgac
     9961 gataaaacaa tctcatgccg ggtcatcaaa acaggcgctg gcgattttgc agggcttaaa
    10021 agcagacgtt gtcacttata accaggtgac cgacgtacaa atcctgcacg acaaaggcaa
    10081 gctgatcccg gctgactggc agtcgcgcct gccgaataat agctcgccgt tctactccac
    10141 catgggcttc ctggtgcgta agggcaaccc gaagaatatc cacgactgga acgacctggt
    10201 gcgctccgac gtgaagctga ttttcccgaa cccgaaaacg tcgggtaacg cgcgttatac
    10261 ctatctggcg gcatggggcg cagcggataa agctgacggt ggcgataaag ccaaaaccga
    10321 acagtttatg acccagttcc tgaaaaacgt tgaagtgttc gataccggcg gtcgtggcgc
    10381 aaccaccact tttgccgagc gtggcttggg cgatgtgctg attagctttg aatcggaagt
    10441 gaacaacatc cgtaaacagt atgaagcaca gggctttgaa gtggtgatcc cgaaaaccaa
    10501 tattctggcg gaattcccgg tggcgtgggt cgataaaaat gtgcaggcca acggtacgga
    10561 aaaagccgca aaagcctacc tgaactggct ctacagccca caggcgcaga ccatcatcac
    10621 cgactattac taccgtgtga ataacccgga agtcatggac aaactgaaag ataaattccc
    10681 gcagaccgaa ctgttccgcg tggaagacaa atttggctca tggccggaag tgatgaaaac
    10741 ccacttcacc agcggcggcg agttagacaa gctgttagcg gcggggcgta agtaatgttt
    10801 gcagtttctt caagacgcgt gctgccgggc tttactttaa gccttggcac cagtctgctg
    10861 tttgtgtgcc tgatattgct gctgccgctc tctgcgctgg tgatgcagct ttctgagatg
    10921 agctgggcgc agtactggga ggtgatcacc aacccgcagg tggtcgcggc ctacaaagtg
    10981 acgctgctgt cggcgtttgt ggcatcgatt tttaacggcg ttttcggtct gctgatggcg
    11041 tggattttaa cacgttatcg ctttccgggc cgcacgctgc ttgatgcgct gatggattta
    11101 ccctttgcgc tgccaacggc agtcgccggt ttaacgctgg cctcgctctt ttccgtaaac
    11161 ggtttttacg gtgaatggct ggcgaagttt gatatcaaag tcacctatac ctggcttggt
    11221 attgcggtgg cgatggcctt taccagcatt ccgtttgtgg tgcgtaccgt gcagccggtg
    11281 ctggaagagt taggcccgga atatgaagaa gcggcggaaa cgcttggtgc aacgcgctgg
    11341 cagagtttct gcaaagtggt gctgccggag ctttctccgg cgctggtggc gggcgtggcg
    11401 ctgtcgttta cccgtagtct tggtgaattt ggcgcggtga tttttatcgc cggaaatatc
    11461 gcgtggaaga cggaagtgac gtcgctgatg atttttgtgc gcttacagga gtttgattac
    11521 ccggcagcga gcgcgattgc ttcggtgatc ctcgcggcat ctctgctgct gctgttctca
    11581 attaacactc tgcaaagtcg ctttggtcgg cgtgtggtag gtcattaatg gcggaagtta
    11641 cccaattgaa gcgttatgac gcgcgcccga ttaactgggg caaatggttt ctgattggca
    11701 tcgggatgct ggtttcggcg ttcatcctgc tggtgccgat gatttacatc ttcgtgcagg
    11761 cattcagcaa ggggctgatg ccggttttac agaatctggc cgatccggac atgctgcacg
    11821 ccatctggct gacggtgatg atcgcgctga ttgccgtacc ggtaaacctg gtgttcggca
    11881 ttctgctggc ctggctggtg acgcgcttta acttcccggg acgccagtta ctgctgacgc
    11941 tactggacat tccgtttgct gtatcgccgg tggttgccgg tctggtgtat ctgctgttct
    12001 acggctccaa cggcccgctc ggcggctggc tggatgaaca taatctgcaa attatgttct
    12061 cctggcccgg aatggtgctg gtcaccatct ttgtgacgtg tccgtttgtg gtgcgcgagc
    12121 tggtgccggt gatgttaagc cagggcagcc aggaagacga agcggcgatt ttgcttggcg
    12181 cgtccggctg gcagatgttc cgtcgcgtca cattgccgaa tattcgctgg gcgctgcttt
    12241 acggcgtggt gctgaccaac gcccgcgcga ttggcgagtt tggtgcagtg tcggtggttt
    12301 ccggctcgat tcgcggcgaa acactttcgc tgccgttaca gattgaattg ttggagcagg
    12361 actacaacac cgtcggctcc tttaccgccg ccgcgctgtt gacgttgatg gcgattatca
    12421 ccctgttttt aaagagtatg ttgcagtggc gcctggagaa tcaggaaaaa cgcgcgcagc
    12481 aggaggagca tcatgagcat tgagattgcc aatatcaaga agtcttttgg tcgcacccag
    12541 gtgctgaacg atatctcact ggatattcct tctggtcaga tggtcgcgtt gctggggccg
    12601 tccggttccg ggaaaaccac gctgctgcgg attatcgccg ggctggagca tcaaaccagc
    12661 gggcatattc gcttccacgg caccgacgtg agtcgcctgc acgcgcgcga tcgtaaagtc
    12721 ggtttcgtgt tccagcatta cgcgctgttc cgccatatga cggtattcga caatatcact
    12781 tttggcctga cggtgctgcc gcgtcgcgag cgcccgaatg ccgcagccat caaagcgaaa
    12841 gtgacaaaat tgctggaaat ggtccagctt gcgcatctgg cggatcgtta tccggcgcag
    12901 ctttccggcg gccagaaaca gcgtgtggcg ctggcgcgcg cgctggctgt agaaccgcaa
    12961 attctgctgc ttgatgaacc gttcggcgcg ctggatgcgc aggtgcgtaa agagctgcgt
    13021 cgctggctgc gtcaactgca tgaagagctg aaattcacca gcgtgtttgt gacccacgac
    13081 caggaagagg cgaccgaagt agccgatcgt gtagtggtga tgagtcaggg gaatattgaa
    13141 caggctgatg cgccgaatca ggtatggcgc gaaccagcga cccgttttgt actcgaattt
    13201 atgggcgaag tgaaccgcct gcagggaacc attcgcggcg ggcagttcca tgttggcgcg
    13261 catcgctggc cgctgggcta cacacctgcg tatcaggggc cggtggatct cttcctgcgc
    13321 ccgtgggaag tggatatcag ccgccgtacc agtctcgatt cgccgctgcc ggtacaggta
    13381 ctggaagcca gcccgaaagg tcactacacc caattagtgg tgcagccgct ggggtggtac
    13441 aacgaaccgc tgacggtcgt gatgcatggc gacgatgccc cgcagcatgg cgagcgttta
    13501 ttcgttggtc tgcaacatgc gcggctgtat aacggcgacg agcgtattga aacccgcgat
    13561 gaggaacttg ctctcgcaca aagcgcctga taggttgagt gaatgttaaa cgcccggagg
    13621 cgcttcccgc gatccgggct ttttaatggc aaggtttgta acctgtaggc ctgataagac
    13681 gcgcaagcgt cgcatcaggc aacaccacgt atggatagag attgtgagta cattagaaca
    13741 aacaataggc aatacgcctc tggtgaagtt gcagcgaatg gggccggata acggcagtga
    13801 agtgtggtta aaactggaag gcaataaccc ggcaggttcg gtgaaagatc gtgcggcact
    13861 ttcgatgatc gtcgaggcgg aaaagcgcgg ggaaattaaa ccgggtgatg tcttaatcga
    13921 agccaccagt ggtaacaccg gcattgcgct ggcaatgatt gccgcgctga aaggctatcg
    13981 catgaaattg ctgatgcccg acaacatgag ccaggaacgc cgtgcggcga tgcgtgctta
    14041 tggtgcggaa ctgattcttg tcaccaaaga gcagggcatg gaaggtgcgc gcgatctggc
    14101 gctggagatg gcgaatcgtg gcgaaggaaa gctgctcgat cagttcaata atcccgataa
    14161 cccttatgcg cattacacca ccaccgggcc ggaaatctgg cagcaaaccg gcgggcgcat
    14221 cactcatttt gtctccagca tggggacgac cggcactatc accggcgttt catgctttat
    14281 gcgcgaacaa tccaaaccgg tgaccattgt tggcctgcaa ccggaagaag gcagcagtat
    14341 tccgggcatt cgccgctggc ctgcggaata tctgccgggg attttcaacg ctgcgctggt
    14401 ggatgaggtg ctggatattc atcagcgcga tgcggaaaac accatgcgcg aactggcggt
    14461 gcgggaagga atattctgtg gcgtcagctc aggcggcgcg gttgctggag cactgcgggt
    14521 ggcaaaagct aaccctggtg cggtggtggt agcgatcatc tgcgatcgtg gcgatcgcta
    14581 cctctccacc ggagtgtttg gggaagagca ttttagtcag ggggcaggga tttaaggatt
    14641 aatagcattg gagactgatg acaaacgcaa aactgcctga tgcgctccgc ttatcaggcc
    14701 tacaatgttt ctgcaatata ttgaattagc aatattttta tgccggatca ggcgtttacg
    14761 ccgcatccgg cataaacaaa gtgcactttg tcaataatct gatttgtcgg cagttgttgc
    14821 tgccgacaaa tgcagttttt aattttcgtg tggaaaagca gtaaggtcat caatcatttt
    14881 ttgaagtatt ggtgagtcct gaccgtcacc ccattgaaga atttttgcgg taagctgatg
    14941 acgcgctagt gcattgagtc ccatcttttt aagtttatgg gagattttac ccatgacttt
    15001 gatctgaccg aatgcagtcg cgtaggtaat catattgctc tggatagagc tcatttcatc
    15061 ggctagtaat tgcgcacgca catcctcttc aagaatactg tccatagcga tgtatttcat
    15121 accccacata gtttcaatta acatttgagg gaaatcagca cagtccagaa cggaggctat
    15181 tttcaagttc gtttagagca tcagcacctt catcgctacc aaaaggagat tcttcatccc
    15241 agcaacacat ataaagtgga tcagtaaaat ggcactggaa gtttgggtgt gacgttttaa
    15301 gatgtaaccc aatctcttca tcatcaaaat agtagagatc catgaagtta aagtctggag
    15361 tttcttgata tccttttttc atttttgcgg caatcaattt actggcttct ttcagacact
    15421 gttcttcatt atcgaattct tttgtatgga atttaccaat actacctacc ttgccatagt
    15481 tgacagctaa actatcccct tcgtaatcta tccaccaaaa tttattcgat ttttcatcgt
    15541 gataaataaa gcattttttc atgttacctc ctgtaaatgc cttaccaaag cggattaatt
    15601 tccttccgca ggcaccgttt tatccgtctg cgcaattaac gcatgtaaaa aaggctgtgc
    15661 gttctcatcg ctcttaccgg ggatcttcac gatatacggc ttcccggtaa tcgatgacga
    15721 cgaagctacc ttatcaataa actgctcggc agtgtcaatg cggttacggg tattgccgag
    15781 cttcagacgc aggtgggaaa ccgcttcatc gcaggtatgt tcatcaccgt tgcgcacaaa
    15841 tatcaaatcc tttttctgtg ctaatccctc cagcatggcg ttgatgcggg cttcttcatg
    15901 aacagttaac ttcgcgtaag cggggagtgt cattagcagc gtaatggcca gacaaatgat
    15961 tttcttcact gaattacctt atctttgttt tgtaatagcc gatttgactg ccagaatcag
    16021 gtaaagtttc ggttggaata cgatgaggat catcggcaag gagaaaaaat taaatgagta
    16081 gtttgttgtt gtttaacgat aagagtaggg cgctgcaggc ggatatcgtc gccgtgcagt
    16141 cgcaggtagt gtacggaagc gtaggcaaca gcattgcggt gcctgctata aaacagaacg
    16201 gcttgaatgt ctttgccgtg ccgacggtat tgctgagcaa tacgccgcat tatgacactt
    16261 tctacggtgg tgcgattccg gacgaatggt ttagcggcta tttgcgtgcg cttcaggagc
    16321 gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta tatgggaacg gcatcgcaaa
    16381 tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga ccatcctgac ctattgatca
    16441 tggtcgatcc ggtgattggc gatattgata gcggaattta tgtcaaacct gaccttcccg
    16501 aggcgtatcg acaatattta ctgccgctgg cgcagggaat tacccccaat atctttgagc
    16561 tggaaatcct gaccggtaaa aattgccgcg atctcgacag tgccattgct gccgcaaaaa
    16621 gtctgctttc agacacatta aaatgggtgg tgattaccag cgcctccggt aatgaagaaa
    16681 atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt gaatgtcatt tcccattcac
    16741 gggtaaaaac cgacctgaaa gggactggcg acctgttttg tgctcagctt atcagtggtt
    16801 tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc ggggttgcgc gtactggaag
    16861 tgatgcgcta cacccagcag catgagagcg atgaattgat tttgccgccg ctggcggaag
    16921 cataaaaaat ggcgccgatg ggcgccattt ttcactgcgg caagaattac ttcttgatgc
    16981 ggataaccgg ggtttcaccc acggttacgc taccggacag tttgatcagt tctttgattt
    17041 cgtccatgtt ggagataaca accggagtca gggtagactt ggctttctct tccagcagcg
    17101 gcagatcaaa ttcaatgaca gtatcgccaa ctttcacgcg ctgaccttct tcagcaatac
    17161 gcttgaagcc ttcgcctttc agttcaacgg tgtcgatacc gaagtggacg aacagttcaa
    17221 cgccgctatc agattcgata gagaacgcgt gattggtttc aaagatttta ccaatggtac
    17281 cgtctacagg cgcgaccatt ttgttacccg ttggtttgat agcaatacca tcaccaacga
    17341 ttttttccgc aaaaacgaca tccggcacgt cttcgatatt gacgatctcg ccagagagcg
    17401 gagcaatgat ctcaatagtt ccggtatcct ttttgtcgtc ggaaaccaga gatttcagtt
    17461 tatcgaacaa acccatgatc ttctcctaag cagtaaattg ggccgcatct cgtggattag
    17521 cagattgttt tttcttcaat gaacttgtta accagcgtca ttaactcgtc cgttgtcggt
    17581 tgagcaagag cctgctctgc taacaccttc gcatcttcga agttcgtgtt acggataatc
    17641 ttcttaatgc gcgggataga aatggcgctc atagagaatt cgtccagacc catccccagc
    17701 aacagaagtg tagcacgttc atcgccagca agctcaccac acatgccagt ccatttgcct
    17761 tcagcatgag aagcatcaat aacttgcttg atcaagttca gcacggacgg tgacattggc
    17821 tggtaaaggt gtgaaatcat atcattacca cggtcaactg ccagagtgta ctgcgttaaa
    17881 tcattggtgc cgatactaaa gaaatcaact tctttggcta aatgacgtgc aattgttgcg
    17941 gcagccggtg tttccaccat tacgccgatt tcaattgact cgtcaaacgc tttaccttcg
    18001 tcgcgcagtt cctgtttgta gatttcgatc tctttgcgca gtgcacgcac ttcttcaaca
    18061 gagatgatca tcgggaacat aatgcgcaat ttaccgaaag ccgaggcacg caggatagcg
    18121 cggagctgat cgcgcaggat ctctttacga tccatcgcga tacggatagc gcgccagccg
    18181 aggaacgggt tctcttcttt cgggaagttc atgtatggca gctctttgtc gccgccgatg
    18241 tccatggtac gaacgataac cgcctgcgag ccacacgctt cagccactgc tttgtaagca
    18301 gcaaactgtt cttcttcagt gggcagcgcg tcgcggtcca tgaacaggaa ctcagtacga
    18361 tacagaccaa cgccttcagc gccgttacgc tctgcacctt caacgtcacg aaccgtacca
    18421 atgttagcgc atacttctac ctggtgaccg tccagcgtaa tagctggcag atctttcagt
    18481 ttagcaagct ctgctttttc agaagccact tgctcctgaa cagcgcgcat tttatcaata
    18541 acttcgttgg ttggattgac gtaaacctga ttatttacgg catccagaat cagatagtcg
    18601 tcatttttca cctgagaggt gacgctaccg gtacccacga tagcaggcag ttccagagaa
    18661 cgcgccatga tagaggtgtg ggaagtacgg ccacccgcgt cggtgatgaa acccagcacc
    18721 ttcttcaggt tcagctgtgc ggtttcggac ggcgtcaggt cagcggcaac cagaatgact
    18781 tcatcctgaa tggcgctcag gtcgataatc ttcaggccca ggatgttgcg cagcaggcgc
    18841 ttaccgatat cacgtacgtc agccgcacgt tctttcaggt attcatcatc cagctcttcc
    18901 agggcagaag cctgaccttc gataacttca tgagcagctg cgtcagctgt catgtgttta
    18961 tctttaatca gggctatgat ttcctgctcc agctcctcat cttcgagcag cataatatgc
    19021 ccttcaaaga tggcttcttt ttcttcaccg aacgtttcac cagctttcgt tttgatcgtt
    19081 tccagctggg ctgatgcctt ggcacgaccg ctcagaaaac gttcaacttc ctgatcaacc
    19141 tggtcggcag aaattttttt ccggtcaatg acaatttcgt cttctttcag aagcagagct
    19201 ttaccgaaag cgatacccgg ggatgctaaa atgcctgaaa tcataaccct accttacttg
    19261 tgactgattt ttaaaagaac ccgggaaatt actcgagttc cgccatcagt ttaaccagat
    19321 gttcaaccgc tttctgctcg tcttcgcctt ctgcggagat agtcacaacg gtaccttgag
    19381 tcaggcccag agtctgcagt ttaaacaggc ttttcgcgct ggcgcttttg ccgttggaag
    19441 tcacagtaat ttcagaagtg aagcccttag cttcttttac aaactgggca gcagggcggg
    19501 tgtgcagacc gttcggagcg gtaatggtaa cttcttgctg gaacattgta tttccccaac
    19561 ttataggttt agtgttgtgg aactaaagtc tagcctggcg gttcgacttt agcctgtatt
    19621 gttagcgcca gtttttaaca gacgcgacgc acgaagagcg caatacagtc cattgctggc
    19681 gggtgttgac cgctgacgtt tcgtacgtca ttaagcatta tgccgctaaa ggaagaattg
    19741 aaccaaatca taaaatcgat tcagctttgg cttttcctgt aacgattaat ttcgcgcatc
    19801 aaaataatta gtctggttaa ataccagttc cgacaggctg aatcaatgcc aggaggggcg
    19861 aaagtttgaa gcaggccaca aaaaagcacc taaaaaggtg cttttttacg catttttaac
    19921 aagctggcat tactgttgca attctttctc agtgaagaga tcggcaaaca atgcggtgct
    19981 taaataacgc tcacccgatg atggtagaat aaccacaata ttcttgttgg taaagctttc
    20041 atcttcttgt agtttcaacg ctgcggcaac agctgctcca gaagagatac ctgcaagaat
    20101 accttcttct tccattagac gacgggcggt agaaatggct tcttcattgg tgatgccaat
    20161 gactttatcg accagcttga gatcgaggtt agccgggata aaaccagcgc caataccctg
    20221 aattttatgc gggccaggtt taatctcttc acctgccagc acctgggcga taactggaga
    20281 atcggttggc tcaacggcga cagagataag atcggtcttg cctttggtgc ctttaatgta
    20341 gcggctgacg ccagtcagcg taccgccagt cccaacgcca gcaataaata catcaacctg
    20401 accgtcggta tcttcccaga tttccggacc ggtggttttt tcgtgaattt cagggtttgc
    20461 cggattgctg aattgttgca gcagcaggta tttctctgga ttgctggcga caatttcttc
    20521 tgctttttgg attgcgcctt tcatgccttt agcaccttcc gtcagcacca ggtttgcacc
    20581 taacgctttc agcagcttgc ggcgttcaat actcatggtt tctggcatgg tcagggtgag
    20641 tttgtaaccg cgagcggcag ctacataggc cagtgcaatc ccggtattac cgctggtcgg
    20701 ttcaaccagt tcaacgcctg gtttcagcac gccgcgcttt tcggcatccc aaatcatgtt
    20761 ggcaccgata cggcacttaa cgctgaagct ggggttacga gattccacct tcgccagaat
    20821 gcgtccgtta ccgatgcgat tcaggcgaac cagcggcgtg tgaccgatag tcagcgagtt
    20881 atcttcaaaa atcttactca tggcctgtcc ttaactgtat gaaattggga tacaacaggt
    20941 agcatacccg ctcagagaat atgcggaagt aaggatttag catatctata tacagaaggg
    21001 aaataatgac atgcaagatg gaataagggg cggcataagc cacccctgtt tcacacaaac
    21061 ggtttacaaa ataaccggta gattgttacc gccacatcgc gtgtttatcg cgatagcaat
    21121 cgacccacat cgccgtcgcg ccacaaacgg caacgggcat gatgaacaga ttaagcagcg
    21181 ggatcatcgt aaacaggctg gttaaagcac caaactgcat attggtgatt ttgcgtgtgc
    21241 gcagggcggt gcgcatctct ttaaacggca ctttgtggtt atcgaagggg taatcgcaat
    21301 actggatggc taacatccag gcgctaaaca ggaaccacag taccggcgcg acggtttgcc
    21361 caataccagg gatgaagtaa agaattagta atacaattgc gcgcggcaga taccagacaa
    21421 atttttgcca ttcgcgtttc atgattcgcg gcacatcttt catgataccg aaaatcccgg
    21481 tatctggcgg tgtagcgcca gtcaatcgtg cttccagttg ttcagccaat aaaccgttaa
    21541 acggagcggc aatccagtta gcaatcgtgg agaagaaata gccaaacact aacagcacag
    21601 agatgacagc cagaggccac aacagataac tcagccattg tagccagtcc ggaacgtaac
    21661 tcatgaaagt cgggatccag acatcgagct gtgtaaagag ccaccagaat gcgcccccca
    21721 tcaacaaaat attgaccagc agcggtaaaa taacgaaacg ccgaatccca ggttgcgaga
    21781 cgagcttcca gccttgcgca aaatagtaaa aaccgctgcg tggggcagat gtgaatgatg
    21841 aaaccataat caggatgagc tccttttgac caatcccagg aaaattctgc gtattttacc
    21901 gggtaattgc gcaatggaca gttaggatat gttcgaaaaa acagcaaaaa gcacgatttc
    21961 atctatcttt gtgctgtgaa agttaatagt gcacttgcac ttgaggtaat cggcaaatac
    22021 tcttagtgag taaatgtttg ccgtggtggc aaggtgttag aacaacagag aatataatga
    22081 tgcaggattt gcgtctgata ttaatcattg ttggcgcgat cgccataatc gctttactgg
    22141 tacatggttt ctggaccagc cgtaaagaac gatcttctat gttccgcgat cggccattaa
    22201 aacgaatgaa gtcaaaacgt gacgacgatt cttatgacga ggatgtcgaa gatgatgagg
    22261 gcgttggtga ggttcgtgtt caccgcgtga atcatgcccc ggctaacgct caggagcatg
    22321 aggctgctcg tccgtcgccg caacaccagt acctaccgcc ttatgcgtct gcgcagccgc
    22381 gtcaaccggt ccagcagccg cctgaagcgc aggtaccgcc gcaacatgct ccgcgtccag
    22441 cgcagccggt gcagcagcct gcctatcagc cgcagcctga acagccgttg cagcagccag
    22501 tttcgccaca ggtcgcgcca gcgccgcagc ctgtgcattc agcaccgcaa ccggcacaac
    22561 aggctttcca gcctgcagaa cccgtagcgg caccacagcc tgagcctgta gcggaaccgg
    22621 ctccagttat ggataaaccg aagcgcaaag aagcggtgat tatcatgaac gtcgcggcgc
    22681 atcacggtag cgagctaaac ggtgaactgc ttcttaacag cattcaacaa gcgggcttca
    22741 tttttggcga tatgaatatt taccatcgtc atcttagccc ggatggcagc ggcccggcgt
    22801 tattcagcct ggcgaatatg gtgaaaccgg gaacctttga tcctgaaatg aaggatttca
    22861 ctactccggg tgtcactatc tttatgcagg taccgtctta cggtgacgag ctgcagaact
    22921 tcaagctgat gctgcaatct gcgcagcata ttgccgatga agtgggcggt gtcgtgcttg
    22981 acgatcagcg ccgtatgatg actccgcaga aattgcgcga gtaccaggac atcatccgcg
    23041 aagtcaaaga cgccaacgcc tgatacactt aaggcaaatt aactcctctt cgaacccccg
    23101 cttgtcgggg gtttttagca ttgatggtgc gatatggaat caatcgaaca acaactgaca
    23161 gaactgcgaa cgacgctttg ccatcatgaa tatctttatc atgtgatgga tgcgccggaa
    23221 attcccgacg ctgaatacga caggctgatg cgcgaactgc gcgagctgga aaccaaacat
    23281 ccagaactga ttacgcctga ttcgcctacc caacgtgtag gtgctgcgcc gctggcagct
    23341 ttcagccaga tacgccatga agtaccaatg ctgtcactgg ataacgtttt tgatgaagaa
    23401 agctttcttg ctttcaacaa acgtgtgcag gaccgtctga aaaacaacga gaaagtcacc
    23461 tggtgctgtg agctgaagct ggatggcctt gccgtcagta ttctgtatga aaatggcgtt
    23521 ttagtcagtg ccgcgacccg tggcgatggc accaccgggg aagatatcac gtctaatgtg
    23581 cgtactattc gcgccattcc gctgaagctg cacggagaga atatcccggc gcgtctggaa
    23641 gtgcgtggtg aagtgttcct gccgcaggcg gggttcgaaa agattaacga agatgcgcga
    23701 cgcacgggcg ggaaagtttt tgctaaccca cgtaatgcgg cagctggttc actgcgtcag
    23761 cttgatccgc gtattacagc gaagcgaccg ctcacttttt tctgctatgg cgttggtgtt
    23821 ctggaaggtg gcgagctgcc ggatactcat cttggccgtt tactgcaatt taaaaagtgg
    23881 ggggtgccgg tcagcgatcg ggtaacgctt tgtgaatcgg cggaagaagt gctggcgttc
    23941 tatcacaaag tggaagaaga ccgcccgacg ctgggctttg atatcgacgg cgtggtgatt
    24001 aaggtcaact cactggaaca gcaggagcag cttggctttg tcgcgcgtgc cccgcgctgg
    24061 gcggttgcgt ttaaattccc ggcgcaggag cagatgacct ttgtgcgtga cgtcgagttt
    24121 caggttgggc gtactggcgc gattacgcct gttgcgcgtc tggaacctgt ccatgttgct
    24181 ggcgtgctgg tgagtaacgc aaccttacac aatgcggatg aaatcgaacg tcttggttta
    24241 cgcattggcg ataaagtggt gattcgccgc gctggcgacg tgatcccgca ggtggttaac
    24301 gtcgtgcttt ctgaacgccc ggaagatacc cgtgaggttg tattcccgac gcattgtccg
    24361 gtatgtggtt ctgacgttga gcgtgtggaa ggtgaagcgg ttgcccgctg taccggtggc
    24421 ctgatttgcg gtgcgcagcg taaagagtcg ctgaaacact ttgtttcccg ccgtgcgatg
    24481 gatgttgacg gaatgggcga caaaatcatc gatcagctgg ttgaaaaaga atatgtccat
    24541 actccggcag atctgttcaa actcaccgca ggcaaactga ccggactgga gcgtatgggg
    24601 ccaaaattgg cacaaaacgt ggttaacgcg ctggaaaaag cgaaagaaac cacctttgct
    24661 cgcttcctct atgcacttgg catccgtgaa gtcggcgagg ccaccgcagc aggtctggcg
    24721 gcatatttcg gcacgctgga agcgctggaa gccgcttcga ttgaagagct gcaaaaggtg
    24781 cctgatgttg gcattgtcgt tgcatcccac gttcacaact tctttgccga agaaagcaac
    24841 cgcaatgtca tcagcgagct gttggcggaa ggtgttcact ggcctgcgcc gatcgttatc
    24901 aacgcggaag agattgacag cccgtttgct ggtaaaaccg tggtgcttac gggcagctta
    24961 agccagatgt cgcgtgatga cgctaaagct cgactggtcg aactgggcgc gaaagtcgcg
    25021 ggcagcgtgt cgaagaaaac cgatctggtg atagcgggtg aagctgcagg atctaaactg
    25081 gcgaaggcgc aggaactggg cattgaagtc atcgacgaag cggaaatgct gcgtttgctg
    25141 ggtagctgag atggaaaaag agcagctgat tgaaatagcc aatacgataa tgccgtttgg
    25201 caaatacaaa gggcgtcgct taattgacct gccggaggag tatctgctgt ggtttgcccg
    25261 caaagatgag ttcccggcag ggaagctcgg tgagctaatg caaatcacgc tgctgattaa
    25321 aaccgagggg ctgacgcaac tggtccagcc cctgaagcgt ccgctttaag ctttatcggc
    25381 gctgctttcc tgctgcgcct gtaactgttc ggtctggcgt ttgtatcgac gcgccagcac
    25441 cgcacagacc atcaattgga tctgatggaa aatcatcagg ggcagcacca tcataccgat
    25501 caccgatgtg gggaacagaa tgtttgccat cgggatgcca tttgccagac tctttttcga
    25561 accacaaaag acgatagtaa tttcatctgc cttattgaag ctcagtcggc gtgccataaa
    25621 gacgttaact acaatcacga tagccagaag aacgcagctg accaccacga taaacagcaa
    25681 tgatccccag ccaactttat gccagatacc attaacgacg gcttcgctga acgctgtata
    25741 aaccaccaac agaatggacg tctggtcagt tttcgcaatc cattttttat tgcgcgacac
    25801 ccagtcacca atccacggcc gggaaagatg ccccaacaca aacggcagca gcagttgcag
    25861 cataatttta ccgacctgct caaggctgcc ccttgcaccg tgaacattca tcaccagacc
    25921 aaccagcaat ggtgaaagga aaatccccag caggctggat gctgacgcag aacaaaccgc
    25981 cgccgcgacg ttaccgcccg ccattgacgt gaaggcgatt gcagactgca cggtagccgg
    26041 gagaatgcac aagtagagaa aaccggagta gagcatcggg tcgacattta ccggtttcca
    26101 ccaggcaaac agtacaccca gaatcggaaa cagcacgaag gtgctgcaca ttacccacaa
    26161 atgcagtcgc cagtgaccac cgccagcaat aatcgcctca cgcgacaact tcgcgccgtg
    26221 cataaagaac agcagggcaa tagctgcggt ggtcagattt tcaaagaagg ggacgaaatc
    26281 gcctctggcc ggaaagaaag aggccagcaa caccaccgtg atcagggtta aggtgaaagg
    26341 atcgaggata cgaaaaagtt tcataaagac tccagatgat cgatggttct attgtgcttc
    26401 ttccagtttg agaaataaaa ttgatttatt gcatccattc atgaagaaaa cagatgaatt
    26461 attctttaaa acaattaaag gttttcgtca cagtagcgca ggagaaaagt tttagtcgtg
    26521 caggagagcg tatcggcttg agccagtcgg cagtaagtca cagtgtgaag gagctggaaa
    26581 atcatactgg tgttcgcctg ctggacagaa ccacgcgtga agtggtgctt acagatgcag
    26641 ggcagcagtt ggctttgcgt cttgagcgac tgctggatga acttaacagc acgttgcgcg
    26701 ataccgggcg tatggggcaa caactgagcg gaaaagttcg ggtcgctgcc agccagacca
    26761 tttccgcgca tcttattccg caatgcattg ctgaaagcca tcgccgctat ccagatattc
    26821 agtttgtcct gcatgatcgt ccgcagcagt gggtgatgga aagtattcgc cagggagatg
    26881 tcgactttgg catcgtcatc gatccaggcc cagtgggcga tctgcaatgt gaagcgattc
    26941 tttccgagcc tttctttctg ctttgccatc gcgatagcgc tttggccgtg gaagattatg
    27001 taccctggca ggcattacag ggagcgaaat tggtgttgca ggattacgcg tcaggcagcc
    27061 gaccgctgat tgacgcagcg ctggcgcgca atggtattca ggcgaatatt gtgcaggaga
    27121 ttggacatcc ggcgacgctg ttcccgatgg tagccgcagg catcggcatc agtattcttc
    27181 ctgcactggc gctaccattg cctgaaggta gcccactggt ggttaagcgc attacgccgg
    27241 tggttgaacg ccagttgatg ctggtgcgca ggaagaaccg ttcactttcc accgccgctg
    27301 aagcgttatg ggacgtggta cgagaccagg gcaatgcgct aatggctggt cgggaagggg
    27361 atccgttgta tcagatatag atatcgatat gagtcgtttt ttcaacagga tttagaaggg
    27421 atacattcag agacaaacgc tgctctttct cctgcgcctt ttctgcctgc tgtttctgca
    27481 actgcgccag ttgtgtttcc agtagggtga tttgctgttg aatcaattcg gcctgttttt
    27541 gcttgtcttc agcactgccg ctagtgtcta caatctcttt aatttgttga gtgagtttga
    27601 tgatttgctg acttatccgc gcgatttgtg aagcaacatc gctaccttgt gatgccctgt
    27661 tactactctg tgtttgtatg gaaggaattg aagttgaaga gatagtggcc attgttcagt
    27721 cctcgtcact gtacgaggta atatcggtag aagaacaagg aacttgagaa gaaaaatcaa
    27781 tcttaca
//
LOCUS       DAJTUN010000043        27732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTUN010000043 DAJTUN010000000
VERSION     DAJTUN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.043"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            303..1073
                     /locus_tag="OO470_001769"
     CDS             303..1073
                     /locus_tag="OO470_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6251041.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1064..1537
                     /locus_tag="OO470_001770"
     CDS             1064..1537
                     /locus_tag="OO470_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6251042.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1644..2183
                     /gene="dnaT"
                     /locus_tag="OO470_001771"
     CDS             1644..2183
                     /gene="dnaT"
                     /locus_tag="OO470_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6251043.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2186..2923
                     /gene="dnaC"
                     /locus_tag="OO470_001772"
     CDS             2186..2923
                     /gene="dnaC"
                     /locus_tag="OO470_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6251044.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2972..3466
                     /locus_tag="OO470_001773"
     CDS             2972..3466
                     /locus_tag="OO470_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6251045.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3720..6011
                     /gene="opgB"
                     /locus_tag="OO470_001774"
     CDS             3720..6011
                     /gene="opgB"
                     /locus_tag="OO470_001774"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6251046.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6149..7171)
                     /gene="lgoD"
                     /locus_tag="OO470_001775"
                     /pseudo
     CDS             complement(6149..7171)
                     /gene="lgoD"
                     /locus_tag="OO470_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7310..8224
                     /locus_tag="OO470_001776"
     CDS             7310..8224
                     /locus_tag="OO470_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6251047.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8439..9800
                     /locus_tag="OO470_001777"
     CDS             8439..9800
                     /locus_tag="OO470_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251048.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9849..11513)
                     /gene="tsr"
                     /locus_tag="OO470_001778"
     CDS             complement(9849..11513)
                     /gene="tsr"
                     /locus_tag="OO470_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6251049.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11632..12078)
                     /gene="hpaR"
                     /locus_tag="OO470_001779"
     CDS             complement(11632..12078)
                     /gene="hpaR"
                     /locus_tag="OO470_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6251050.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12350..13639
                     /locus_tag="OO470_001780"
     CDS             12350..13639
                     /locus_tag="OO470_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6251051.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13636..15102
                     /gene="hpaE"
                     /locus_tag="OO470_001781"
     CDS             13636..15102
                     /gene="hpaE"
                     /locus_tag="OO470_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6251052.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15104..15955
                     /gene="hpaD"
                     /locus_tag="OO470_001782"
     CDS             15104..15955
                     /gene="hpaD"
                     /locus_tag="OO470_001782"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6251053.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15965..16345
                     /locus_tag="OO470_001783"
     CDS             15965..16345
                     /locus_tag="OO470_001783"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6251054.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16413..17216
                     /gene="hpaH"
                     /locus_tag="OO470_001784"
     CDS             16413..17216
                     /gene="hpaH"
                     /locus_tag="OO470_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6251055.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17227..18030
                     /gene="hpaI"
                     /locus_tag="OO470_001785"
     CDS             17227..18030
                     /gene="hpaI"
                     /locus_tag="OO470_001785"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6251056.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18052..19407
                     /gene="hpaX"
                     /locus_tag="OO470_001786"
     CDS             18052..19407
                     /gene="hpaX"
                     /locus_tag="OO470_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6251057.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19417..20307
                     /gene="hpaA"
                     /locus_tag="OO470_001787"
     CDS             19417..20307
                     /gene="hpaA"
                     /locus_tag="OO470_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6251058.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20334..21271
                     /locus_tag="OO470_001788"
                     /pseudo
     CDS             20334..21271
                     /locus_tag="OO470_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21498..23056
                     /gene="hpaB"
                     /locus_tag="OO470_001789"
                     /pseudo
     CDS             21498..23056
                     /gene="hpaB"
                     /locus_tag="OO470_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23074..23586
                     /locus_tag="OO470_001790"
     CDS             23074..23586
                     /locus_tag="OO470_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6251059.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23979..26129
                     /gene="btsT"
                     /locus_tag="OO470_001791"
     CDS             23979..26129
                     /gene="btsT"
                     /locus_tag="OO470_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6251060.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26179..26382
                     /locus_tag="OO470_001792"
     CDS             26179..26382
                     /locus_tag="OO470_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6251061.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26393..27349
                     /gene="yjiA"
                     /locus_tag="OO470_001793"
     CDS             26393..27349
                     /gene="yjiA"
                     /locus_tag="OO470_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6251062.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27418..>27732)
                     /locus_tag="OO470_001794"
     CDS             complement(27418..>27732)
                     /locus_tag="OO470_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6251063.1"
                     /translation="TGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGS
                     KNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWGLAELKMRHEKMKTQLKKLW
                     LV"
BASE COUNT         6727 a         7294 c         7026 g         6685 t
ORIGIN      
        1 aatcaatatt tattcaataa aatctgatga ttttaacttg ttaacactaa aaaactcgcc
       61 cagctatcta ttatcctgac ccgtaaaggc taaacaataa attcattata gtgataatat
      121 tcatgtgata gtttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttgtccca
      181 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca
      241 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      301 ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg tatccagtgt ggattatttc
      361 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      421 cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacta
      481 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      541 atgtggtgac tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca
      601 aaggcgtaga gaaacgattt agccaaattc aaccattacg ttacccaaga tggctggtag
      661 ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg
      721 gtgccgtcat caccttcttt gccagtacga ccgcgatgta tatccgccag ctgctggcac
      781 aacgtcatct tcatccacag atcaactttt gccttaccgc tttcgccgcc accaccattt
      841 ccggattgct tttgcaactc cccactttca gcaatacccc caccattgcg atggccgcca
      901 gcgttctgct gctagtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag
      961 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct
     1021 gcgtcggcgt agtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
     1081 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
     1141 ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg ctgcttggct cgataggtca
     1201 tggttcccga atgatcttga tgaccagcgg gttgaatatt gagtggtcaa cctttatggc
     1261 ttctatgctg gtcggtacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
     1321 agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat
     1381 gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga ttaccctgtt
     1441 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
     1501 tagattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg
     1561 atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca
     1621 tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta
     1681 ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg
     1741 ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac
     1801 tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga tgtcgctctg gacgaccaac
     1861 tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc gccatgtatc
     1921 cggactggca acccgatgcc gattttatcc gcctggcggc gctatggggc gtggcgctaa
     1981 gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta
     2041 aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg
     2101 ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagtgaacct gacagccaaa
     2161 ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca
     2221 aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg
     2281 gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa
     2341 aatgcagcgc acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga
     2401 gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt
     2461 cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa
     2521 aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat
     2581 catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcggtaccag
     2641 cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg
     2701 cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc
     2761 ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt
     2821 gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttatggg tgatcttcaa
     2881 ctgggatagc taccgtagcc gggtaacggg taaagagtat taatccccgt ttctggcggt
     2941 atactgcgac acaattccac cttgagtaat gatgatgaaa actattaaac atcttctgtg
     3001 ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga
     3061 tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg
     3121 ttggtcactc gcgtcattaa ctaacttgct tagcagcgga aaccaagcct taagcgcaga
     3181 taacatgaac aatgccgcag gcattctgca atactgcgcg aagcaaaagc tggcttcggt
     3241 aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga
     3301 gcaaaaagaa gacaccaact atctggacgg tattcagggt ttgctgaaaa caaaagatgg
     3361 tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg tgaaaaccaa
     3421 agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaacgccg
     3481 cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg
     3541 gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta taaactaatt
     3601 cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat
     3661 tgtgcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccat
     3721 tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag
     3781 cgggacgtaa cacctggtgg tttgcagcca cgttaacggt gctggggcta tttgtcgttt
     3841 taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac gacgcggttc
     3901 tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc ctgccgggta
     3961 tcggcattgt gctggggctg acggcggtgt tcggtgcgct gggctggatc ctgcgccgtc
     4021 gtcgtcatca tccgcaccat tttggttaca gcctgctggc gctcttactg gcgctgggtt
     4081 cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg
     4141 atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga
     4201 aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgaag
     4261 ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc
     4321 acacgcagca gctgccagga acggattaca cgattgcggg catggtggct tctcagtgcg
     4381 gcataccgct gttcgccccc tttgaaggca acgcctccgc ctctgtctcc agcttcttcc
     4441 cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc
     4501 agggcgcaaa tctgcgtttt gccggtaaag atgtgttcct gaaatcgcac ggcttcgacc
     4561 acttatacgg ctcagaagag ctgaaaagcg tggtagccga cccgcactat cgcaacgact
     4621 ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa gagctttccc
     4681 gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt
     4741 ttatctcgcg cacctgtaac cgcaaaaaat atgattttga cggtaagccg aatcagtcat
     4801 tcagcgcggt aagttgcagc caggagaata tcgcgacgtt tatcaacaaa atcaaagcgt
     4861 caccgtggtt taaagatact gtcatcgtcg tctcttctga ccatttagcg atgaacaaca
     4921 cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc attcgtggcg
     4981 acaagccgca gcaaaagacg ctggcagtga agcgtaacac gatggataac ggcgcgacgg
     5041 tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc
     5101 agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccagata
     5161 tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa
     5221 acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca
     5281 aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc cagctggccg
     5341 atttcgctcc acgcgacaat ttcgtctggg ttgacagttg ctacaagatg gcacaactct
     5401 gggctccgga actggcactt tccaccgact ggtgtgtctc gcaggggcag cttggcggtc
     5461 agcaaattgt tcagcatgtt gacaaaacaa catggaagag caagacggca tttaaagata
     5521 cggtcatcga catggcgcgt tacaaaggca atgtcgatac gctgaagatt gttgataacg
     5581 atattcgcta caaagccgac agtttcatct ttaacgtcgc cggtgcgcca gaagaggtga
     5641 aacagtttag cgggatttcc cgtccggagt cgtggggccg ctggtccaac gcgcagctgg
     5701 gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta
     5761 ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag
     5821 aacaaactct tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa
     5881 ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata
     5941 tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gtggtagaac
     6001 gtgaagggta attcctgact gatgtgtagg tcgcataggg tgcgacctac acgccttctc
     6061 cggcttacaa cacattatta agaaacaaaa aaaacagcgc ccaacacttc cccggacgct
     6121 gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta atcaactcac
     6181 gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga tagcgatggg
     6241 ttaacatcat gtcggcagtg attttccctt ccgccatcag tcgaccgact ttagcgaaat
     6301 cttccggcgt ggcgttgcgg ctgcccatca tcgtcgtttc ttttttatgg aattccggat
     6361 cggagaactg caactcacct ttaaacagac cgacaaatac caccgtgccg ccgtgacgaa
     6421 tcagattcac ggtattattc atcgcatgtt gattacctgt cgcgtcgatc actttctgcg
     6481 ccagcgaacc accaaactgc gcccgcagct gcgcgtcaaa atcttcggct aacgggtcca
     6541 gcacgggcaa ctccagacgc gttgccacat gttcacggcg cgccggactg gtatccgcca
     6601 ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg attggccccg
     6661 ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc gcatgagcgc
     6721 taatagcgaa aggttcaatc aatgcggccg cctgcgggtc aataccgtct gcgggcaaaa
     6781 tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc acgccaatga
     6841 ctgaaatttt ttcacagcag ttggtacgcc cgcttttaca cgccgggcat tgctgacagg
     6901 caacataagg gatcacagca acttgctgac catttttaag atcagcaata tttttaccca
     6961 gcccaacaat ctccccacat atttcatggc ctaaaacccg tggataacta aaaaatggtt
     7021 gatttccacc ctaggcatga atatcggtac cgcaaatccc gacagactta atttttatta
     7081 atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat tctttcggct
     7141 gctggcaaat taaaacattc atcgtagaca tgattcggct atccattttt tgttgcctta
     7201 gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg aaaacacacg
     7261 cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata tgagtcgttc
     7321 acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc ggggtcatat
     7381 cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca gccgcactac
     7441 agtgcgtcat attctcagcc aattacgcga atgcggcgtc ttaacgcagg ttggaaacga
     7501 ctatgttatt gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta ccgcttcaat
     7561 gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg atgataaacc agcgtcagtt
     7621 acgccctagg gaaacttttt ctgaactgca actggctcgg gccgcgggtg tcagcccggt
     7681 ggttgtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa gcgaaaagcg
     7741 cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt ttgagctacg
     7801 cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc atgatccccg
     7861 ctggctgcag gcgaaaacca tgctagaacg tcatcggcta ctgcgtgaca acatcggcaa
     7921 tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc tttcagctgc
     7981 cgacaatatc ttttttgatc aatcgcttga gatcatctca gtcatctttc attttcacta
     8041 tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg aacatatgac
     8101 cattctcagc gctctgattt gccgcagtga ccttgatgcc acccttgcgc tccgtaacca
     8161 tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc gttatgctca
     8221 ttaagtacgg attaaaaaca gcaaactatt agcgggtgca cggttttatc aaaagataaa
     8281 taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatg tttttgtgag
     8341 cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt aatgcacaat
     8401 tacgtaaaca attaaaatta taaatacagg agtccggcgt ggaaaaagaa aatatcaccc
     8461 tcgatccgcg ttcttcattt actccatctt cgtcggcaga tattcccgtg ccaccagatg
     8521 gattagttca acgcagtacc cgaattaaac gcattcaaac caccgccatg ttgttattat
     8581 tttttgcggc ggtaatcaat tatctcgacc gcagttcgct gtcggtagca aatttaacga
     8641 ttcgtgaaga attgggatta agtgccaccg aaatcggcgc tttgctctcc gtgttttcac
     8701 tcgcttacgg cattgcgcaa cttccttgcg gcccactatt ggatcgtaaa ggcccacgcc
     8761 tgatgctggg actggggatg ttcttctggt cactgttcca ggccatgtct ggcatggtgc
     8821 acagctttac gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa gcgccgatga
     8881 acccatgcgg tgtaaaagtc attaacgact ggttcaacat caaagagcgc ggacgcccga
     8941 tgggcttctt caacgcagct tctaccattg gcgttgctgt aagcccaccg attctggcgg
     9001 caatgatgct ggtgatgggc tggcgcggga tgtttattac cattggtgta ctggggattt
     9061 ttctcgccat cggctggtat atgctctatc gcaaccgcga gcacgtagaa ctgactgccg
     9121 ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg ccgagatccg ctcaattttg
     9181 ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg aatgatgctc ggattcagtg
     9241 gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc tggttacctg caaacagcct
     9301 ataacctgga tttaaaaagc acagggttga tggcggctat ccctttcctg tttggggctg
     9361 ccgggatgct ggtcaacggt tacgttactg actggctggt caaaggggga atggctccga
     9421 ttaaaagccg taagatctgc attattaccg ggatgttctg ttctgccgcc tttacgctga
     9481 tagtaccaca agcgacaaca tccatgatgg cggttctgct gattggcatg gcactgttct
     9541 gtattcactt tgccggaaca tcctgctggg gcttgatcca cgtcgcagtt gcttctcgca
     9601 tgactgcgtc ggtgggcagt atccagaact ttgccagctt catctgcgcc tctttcgcgc
     9661 cgatcattac tggttttatt gttgatacaa cccactcatt ccgtctggca ctaatcattt
     9721 gcggttgcgt caccgcagcg ggagcactgg cgtacatctt cctggttcgc cagccgatca
     9781 acgacccgcg taaagattaa actccctatg cccgataaca ttttgcttat cgggcatttt
     9841 tatggcgatt aaaacgtttc ccagttctcc ccgctatctg ccactgccat tttacgcggc
     9901 gtagctggcg tcacggtttt taccacagcc gatgtttcac gctgctgttg ctgctgttgc
     9961 tgaatccgga acactgccac agcttcggtc aggcgactgg cctgctcttc cagcgcggcg
    10021 gcggcagcgg cagattcttc caccagcgcg gcgttctgtt gagttacccg gtccatctca
    10081 gcaaccgcta agccaacctg atcgatacca cggctctgct catcagaagc agaagctatt
    10141 tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa tctccgccat tgtttccccg
    10201 gcgctttcga ccagcgtaga gccaacatcc actttcccca ccgagtcttc aatcaggctt
    10261 ttaatttcac gaaccgcctg ggcgctacgc tgggccagat tacgcacttc tcccgcaacc
    10321 accgcaaaac cacgtccttg ctcacccgca cgcgctgctt caaccgccgc gttcaaagcc
    10381 agaatattgg tctggaaggt aatgccgtca attacgctga taatatcggc gattttctgc
    10441 gaactggtgg agatatcgcg catagtctgc accacgttat ctaccacttt gccgccgcgt
    10501 tgcgccgttt cagaagcact taatgccaga tggctggcct ggcgcgcatt ctcggcgttc
    10561 tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg tctcttccag cgaagcggcc
    10621 tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag cgatttcgct ggcaccgcta
    10681 tagatggcat tggccccgtt gcgcacatca ccgacggtac gcatcagctc tccctgcata
    10741 tggcgcaaac tctctgccag ttgccccatc tcattagagc catccacctc aatcggtttc
    10801 accagatcgc cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat tggcgctacc
    10861 agcgaggctt taataccgaa ccagacggcg aagatgaccg ccagtacgac gatcatcacg
    10921 cccaccagaa tccacatcgc ctggctgtag gaggcattgt tatcgctgac ggcgatatca
    10981 tagagccgat cgttttgctc catgtaagcc acatactgct tctcgaaacc gtcctgatat
    11041 ccctgggtcg gctgatcaaa gaactcgttg attttgcctg cacctaacag ttggatcagc
    11101 tccgccagcg cattgtgata aatatcgtaa ttacgtttga tctctgccgc tgcggcggtg
    11161 ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc agtttttttc cgcctgtttc
    11221 agcgaaatac tggcactctg catcagctca gcaacggttg aaccgctacc aatattattc
    11281 tgatccatca tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg caacaacgcg
    11341 acccagctgc cattcagcgt gggttgctgc tggcgaatgg tttgtaaaac agtgaaattt
    11401 tctttgtcat tctttaaggc attaaaaaac agaccgcctg atgtcagttg taaaaggcca
    11461 aaaacggcca aaaccagcag taagctggtc acgatcttga tacgttttaa catggtttct
    11521 ctttcctgtg gaccgatgca agattttcgg cctggaaagc aaaaacttta tgaaaaggtg
    11581 taacccgcca tccatggaaa aaccaggcgc tggttatcag atactaaaaa gttattcatt
    11641 atcgcccggg atgtcttctt gtcgggaatt gcccagagca ataaattcct ctaacagatg
    11701 ggttaactgt tgcattttct cggcagtaaa ctgagcttca atctgccgat aggcttcttc
    11761 aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct ttggtcagcg agatatagag
    11821 cttccgctga tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt ccatccgcgt
    11881 gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat cgtgaaaatc
    11941 cattgatggg ctttccgcca gaatacgcac gatgcgccac tgttgctcag tcaaattatg
    12001 ccgtttaacg attgggcgaa aataactcat cgccgcttct cgcgcctgga gcaacgcaat
    12061 ggttagtgag tcgtgcatta tctttcccct gaaaaattca aaatcattaa tatagaaaca
    12121 attaattatc acgttaccgg gactgagctg cccccgcaat tcttcatttt atcaaaattg
    12181 atgatatttt attaacttac tgatatttaa tattttatta tctttaaatg taaatagttt
    12241 gttaattaga tcacatttat atcacttact tttgccaaac tacgcaatcg ggaataaaag
    12301 taatcattaa catattaatg attaagatga tcgaatccga ggagtggtaa tgaaaggcac
    12361 tatcttcgcc gtagcgttga accatcgcag ccaacttgat gcctggcagg aagcgttcca
    12421 gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg tttattaaac cgcgcaatac
    12481 ggtgattggt tgcggtgaac cgattccctt tccacagggt gaaaaagtac tgagcggtgc
    12541 gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta cgtgaagaag atgcggcaga
    12601 gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg ccggaagaga gcttttaccg
    12661 tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa ccgtggctct
    12721 cagcaatgtc gataatctga ctatctatac cgagatcaac gggcgtcctg ccgatcactg
    12781 gaataccgcc gatttacaac gtaacgccgc acagctgctg agcgccctga gcgaatttgc
    12841 cacgctgaat ccaggcgatg ctattctgct cggcacgcca caggcgcgcg tggaaataca
    12901 gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg ccgctggaaa atccggtagt
    12961 ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg ctgccacacc cgcacggtac
    13021 gctgtttgcc ctcggcctga actacgccga ccacgccagc gaactggaat ttaagccacc
    13081 ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc actggcgaca accagacctc
    13141 cgtgcgcccg aacaatattg aatacatgca ctacgaagcg gagctggtgg tagttattgg
    13201 caagcaggcg cgtaacgtca gcgaagccga tgccatggat tatgtcgcgg gctacaccgt
    13261 gtgtaacgac tacgccattc gcgactatct ggaaaactac taccgcccta acctgcgggt
    13321 caaaagccgc gacggactga cgccgatgct ttcaaccatc gtgccgaaag aggcgatccc
    13381 ggccccgcat aatctgaccc ttcgcacctt cgtcaacggc gagttacgcc agcaaggcac
    13441 caccgccgat ctgatcttca gcgtgccctt cctgatcgcc tacttaagcg aatttatgac
    13501 cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa ggcttatctg acgtggtgcc
    13561 tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc gaattgtgag
    13621 tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa tgttgcaggt
    13681 aacgactact tccagactac caatccggca acgggtgaag tgctggcgga tgtggcctct
    13741 ggcggtgaag cggagatcaa tcaggcggta gcggcagcga aagaggcgtt cccgaaatgg
    13801 gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga tctgatcgac
    13861 cagaacgtgt ctgagatcgc cgcgatggaa accgcggaca cgggcctgcc gatccatcag
    13921 accaaaaatg tgttgatccc acgcgcttct cacaactttg aatttttcgc ggaagtctgc
    13981 cagcagatga acggcaagac ctatccggtt gacgacaaga tgctcaacta cacgctggtg
    14041 caaccggtag gcgtttgtgc gctggcgtcg ccgtggaacg tgccgtttat gaccgccacc
    14101 tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg tgctgaagat gtcggaactc
    14161 tccccgctga ccgccgaccg cctgggtgag ctggcgctgg aagctggtat tccggcgggc
    14221 gtgctaaacg tggtacaggg ctacggcgca accgcaggcg atgcgctggt ccgtcatcat
    14281 gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg ggcgcaacat catgaaaaac
    14341 gccgggctga aaaaatactc catggagctg ggcggtaaat caccggtgct gatttttgaa
    14401 gatgccgata ttgagcgtgc gctggacgcc gccctgttca ccatcttctc aatcaacggc
    14461 gaacgctgca ccgccggttc gcgcatcttt attcagcaga gcatctatcc agaattcgtt
    14521 aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga tccgaatacc
    14581 caggttaggg cgcttatcag tcagcaacac tgggaaaaag tctccggcta tatccgtctc
    14641 ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg ataaaccgtc cgacctgcct
    14701 gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc tggcagacgt agataaccgt
    14761 atgcgcgttg cccaggaaga gattttcggg ccggtcgcct gcctgctgcc gtttaaagac
    14821 gaagccgaag gcttacgcct ggcaaacgac gtggagtacg gcctcgcgtc gtacatctgg
    14881 acacaggatg tcagcaaagt gttacgcctg gcgcgtggca ttgaagcagg catggtgttc
    14941 gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg gcggcgtaaa agcctccggc
    15001 accgggcgtg aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa gaacgtctgc
    15061 atttccatgg gcgaccatcc aattccgaaa tggggagtct gatatgggta agttagcgtt
    15121 agccgccaag atcacgcacg taccgtcaat gtatctctct gaactaccag gaaaaaacca
    15181 cggttgtcgc cagggtgcga tcgacgggca taaagagatc agcaagcgtt gccgggaaat
    15241 gggcgtcgat accattatcg ttttcgatac tcactggctg gtcaacagtg cttatcacat
    15301 caactgtgca gaccattttg aaggcgtcta caccagcaac gagctgccgc attttattcg
    15361 cgacatgacc tacaactacg agggcaaccc ggagttgggg cagctaattg ccgaggaagc
    15421 cttaaagctc ggcgtgcggg caaaagcgca caatattccc agcctgaaac tggaatacgg
    15481 cacgctggta ccgatgcgct acatgaatga agacaagcac ttcaaagtgg tctccatttc
    15541 ggctttctgc acggttcacg attttgccga cagccgcaag ctgggcgaag cgattctgaa
    15601 agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt tatcgcaccg
    15661 ctttattgac gatcagcgtg cggaagaagg gatgaacagc tacacccgcg agttcgaccg
    15721 ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc cagttcaaag agttttgcaa
    15781 tatgctgccg gagtacgccg actactgcta cggcgaaggc aatatgcacg acacggtgat
    15841 gctgctgggg atgctcggct gggataaata cgacggcaag gtggagttta ttaccgagct
    15901 gttcccaagc tctggcaccg gtcaggttaa cgctgttttc ccgctgcccg cgtaaggacg
    15961 ttttatgccg cactttatcg tggaatgtac tgaaaatatt cgtgaagaag cccgcttacc
    16021 tgagctgttt gccagtgtta acactgcact ggcggcaacg gggatttttc cgcttggggg
    16081 aatacgtagc cgggcgcact ggatcgatac ctggcaaatg gctgatggca agcacgatta
    16141 cgcctttgtg catatgacgc taaaaatcgg cagcggacgc agtctggaaa gccgtcagga
    16201 agtgggcgaa atgctgtttg atctgattaa aacgcacttc gcctcactga tggagagccg
    16261 ttacctagcg ctttcatttg agattgcaga gctgcacccg acgttgaatt tcaaacagaa
    16321 caacgtacac gcgttgttta aataaatttg caccagcttc caggccggat gataaggcgc
    16381 tgccgcatcc ggcaatgcca caggatatcg ctatgttcga taaacacacc cacaccctga
    16441 tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg atctcgctgg
    16501 attacccgga gatcaccatc gaagacgcct acgccgtgca gcgcgaatgg gttcgcctga
    16561 aaatcgccga aagtcgcacg ctgaaaggcc acaaaatcgg tctgacctcg aaagcgatgc
    16621 aggcaagctc gcagatcagc gaaccggatt acggtgcact gatggacgac atgttcttcc
    16681 acgatggcag cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa gtggagctgg
    16741 cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac gctgttcgac gtttacaacg
    16801 ccacggacta tgtgatcccg gcgctggagc tgatcgacgc tcgctgccac aacatcgatc
    16861 cggaaaccca gcgcccgcgc aaagtattcg acaccatttc cgataacgcc gccaacgccg
    16921 gagtgatcct cggaggtcgt ccgatcaaac cagacgaact ggatctgcgt tggatctccg
    16981 ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt cgccgctggt gtgctgaatc
    17041 atccggcaaa cggcgtggcc tggctggcga acaaactcgc accgtatgac gtacaactgg
    17101 aagccgggca aatcattctc ggtggttcgt tcacccgccc ggttccggcg cgtaagggcg
    17161 acaccttcca cgtcgattac ggcaacatgg gctccattag ctgccgcttt gtttaaggag
    17221 agaacgatgg aaaacagttt taaagcggcg ctgaaagcag gccgtccgca gattggatta
    17281 tggctggggc tgagcagcag ctacagcgcg gagttactgg ccggaccagg attcgactgg
    17341 ttattgatcg acggtgagca cgcgccgaat aacgtgcaaa ccgtgctcac ccaactacag
    17401 gcgattgcgc cctaccccag ccagccagtg gtacgcccat cgtggaacga tccggtgcaa
    17461 atcaaacaac tgctggacgt aggcacacaa accttactgg tgccgatggt acaaaacgcc
    17521 gacgaagccc gtaaagcggt acgcgccacc cgttatcccc ccgccagtat tcgcggtgtg
    17581 ggcagtgcgc tggctcgcgc ctcgcgctgg aatcgcattc ctgattacct gcaaaaagcc
    17641 aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa gaacttaccg
    17701 cagattctgg acgtggaagg ggtcgacggc gtgtttatcg gcccggcgga tctgagcgcc
    17761 gatatgggtt atgccggtaa tccgcagcac ccggaagtac aggccgccat tgagcaggcg
    17821 atcgtgcaga tccgcgaagc gggcaaagcg ccggggatcc tgatcgccaa tgagcaactg
    17881 gcaaaacgct atctggaact gggcgcgctg tttgtcgccg tcggcgttga caccaccctg
    17941 ctcgcccgcg ccgccgaagc gctggcagca cggtttggta atagttcttc tatttcgatg
    18001 gcgaagcaaa acaacaactc tgtctactaa acgtacgatg tggagcgcat catgacgacc
    18061 tctaccctgc aatataaaga taataaaact gttgaaattg aacatcgtgt cattaataaa
    18121 ttatttcgtc gcctaatcat ttttcttttt attctgtttg ttttttcgtt tcttgatcgc
    18181 atcaatatag gatttgccgg actgacaatg ggcaaggatc ttggcctgac gtccacaatg
    18241 tttggcctcg ccgcaacact cttttatgtc acgtacgtca tgtgcgggat ccccagtaat
    18301 atgatgttag cgaaaattgg cgctcgtcga tggatcgctg ggatcatggt ggtctgggga
    18361 attgcttcca cctgcacaat gtttgcaaca agccccgaaa cactctatct actgcgtatg
    18421 ctggttggta ttgctgaggc gggtttttta cctggtattt tggtttatct gacctggtgg
    18481 ttcccggcat atcatcgggc cagagccaat gccttattta tgatagcgat gccagtcact
    18541 atgatgctgg gattgatcct ttcaggctac attctggcct tggatggact gtggaatcta
    18601 aaaggctggc aatggctctt tttgctggaa ggtctgccat cggtcattct tggtgtcgtc
    18661 acctggtttt atctcaacga cacaccagac caggcgacct ggcttgatga aaacgaaaaa
    18721 caggctttaa aaacgatgat tctccgtgaa caagagagtg catttgccca tgcatctgtt
    18781 ccacgctcaa ccttacgtga agtcctgacc ccagccatca tgctatatac acttgcttat
    18841 ttttgcctga ctaatactct gagtgcgatt aatatctgga cgccgcaaat cctacagagt
    18901 ttcaatactg gcagtagcaa catcatgatt ggtttgctgg cggccatccc ccaattctgc
    18961 acaatttttg gcatgatctg gtggagtcgt cgttccgacc gtcttaaaga acgcaaaaag
    19021 catacgattc ttccctacct gtttgccgct gccggatggt tactcgcttc tgcgacagat
    19081 cacagcctca ttcaactgag cagcatcatt atggcctcaa tgggatcatt taccgcgatg
    19141 actatctttt ggacaacacc ggatcaagtt ataagcttgc aatcgcgtgc cgttgcgatg
    19201 gctgtcatca atgctgtcgg caatgtaggt tcagcagtca gtccattact tatcggtatt
    19261 ttgaaagatg caaccggcag ttttagctca ggtttgtggt ttgtagcagg attgctggta
    19321 ctgggcgcgt tagtcatgac ccgaatccct atggggagtc gggaacactc tctttcatct
    19381 gaacttatcg caccaaaacc gcaataagga gtcatgatgt ccagtgaagc tatctttgaa
    19441 catggttatg aaaatattga tttggtccgg gaatatgatg cccgctacgc tggtgaagat
    19501 gtgcattatg aaacatttgc ccggttagcg aatttctttg gacgcgatat gcgccctcac
    19561 tggcacgata gatattttca actccattat ctggtcactg gacgcattac tttacaactg
    19621 gatgagcact tctattctgt gcgcgcaccg ctttttgtac taacaccacc atcggtgcca
    19681 catactttct ttactgatcc tgatagcgat gggcatgttc tgaccgtacg tcaggagtta
    19741 atttggcccc tggtagaaaa gctctggcct ggtagtggag atgccgcaat gctacaaggt
    19801 atctgtctgt cactggaggg aatggaacat acacaggcgg cattaaatca ttattggccg
    19861 ataatatccc tggagtttca acaaaataag caaggacggg aaattctatt gcaatcactg
    19921 gcgcaaagta tttttacaat cctactgcgt gaagcaccac caaatgatat ttcaacatgc
    19981 agcgtgcgcg gcgagatgtg tttattccag aagtttaatc ggctgattga cgaaaaatat
    20041 cgccaacatt taatggtccc tgaatatgca acaatgctcg gtatgagtga atcacggctt
    20101 accgagctgt gccgacgttt tgccaatcaa tcgccgaaga gattgatttt cgaacgagtg
    20161 agccgggaag ccagacggct tctgctctat agcgagcaaa gcatcaacca gatagccctt
    20221 gatcttggct acaaagatcc cgcctatttc gcccgttttt tcaaccgaat gatgggttgc
    20281 ccaccaacac aattccgtgg tcgctgacca tttatcaacc tgagagactc gacatgaaaa
    20341 tatctttacg ctcactattt gtggcgtgcc tgattgcgcc agtggcattc acctacgcct
    20401 ccgaaccgca ataaatgaaa attgtactac ttggaacgaa aggtggccct tctttattaa
    20461 aagctgaccg tttaccgatg gcaacggcac ttattattgg caataaaacc tagttaattg
    20521 atgctggtta cggggccagc ttacaactgg taaaaaataa tattccactg cgccagattg
    20581 ataatatttt actcacccat ttacacagtg atcatattct ggactatcca tcagtcttaa
    20641 tgaatgcatg ggctagcggt ctgcaggaca aaactattca tgtattcggt ccgccaggca
    20701 cccgagccat gaccgctgcc agttggcagg tcttcgatcg ggacatcaca ctgcgtatgg
    20761 aagaagaagg aaaacccgat cctcgcaaac tcgttacagc tcaagatgtt ggtgaaggcg
    20821 tagtttataa agatgatacc gtgaccgtga ctgcattaaa ggtccctcat tctcctttcc
    20881 ctgacggtga ggcttttgct tatcgcttcg acacacaagg caagcgaatt gtcttctcag
    20941 gagatacctc gtactttccc ccactggcaa ctttctcaga aggcgctgat atacttgtgc
    21001 atgaggccgt acatgagccc tccgtcgcta agctggcaga cagtattggc aatggtaaaa
    21061 cactggcaaa agccattgct tctcaccata cgactattga ggatgtaggc aaaattgcca
    21121 atgaagcaaa agtgaaaaaa ctcatcctta gccacctggt accaatcacc gttccggatg
    21181 aaacatggaa gcaagaagca cagaaaacta ttcaggccct atcgtcgttg gtcatgaaaa
    21241 tatgacgatt accattccat tcgccccata aaaaattccg tccccgttag cccttgttag
    21301 cggggaatat tatgatttgc cttactgtaa gttgatcacg atcgcagttt actatttaac
    21361 tccaccttgc tgtcgaaaag taccaacgtt cgcctgaaac gtctcttccc gacaaccgcg
    21421 ttcaacgctt caattaaata acaaaaacaa cacacaaaat taacaatcgt attccgatta
    21481 atactgtaga ggtcgacatg aaaccagaag atttccgcgc cagtacccaa cgtcctttca
    21541 ccggggaaga gtatctgaaa agcctgcagg atggtcgcga gatctatatc tatggcgagc
    21601 gagtgaaaga cgtcaccact catccggcat ttcgtaatgc ggcagcgtct gttgcccaac
    21661 tgtacgacgc gctgcacaaa ccggagatgc aggactctct gtgttggaac accgacaccg
    21721 gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa aagtgccgac gacctgcgcc
    21781 agcaacgcga cgccatcgct gaatggtcac gcctgagcta tggctggatg ggccgtaccc
    21841 cagactacaa agccgctttc ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt
    21901 tcgagcagaa cgcccgtaac tggtataccc gtattcagga aactggcctc tactttaacc
    21961 acgcgattgt taacccaccg atcgtcattt gccgaccgat aaagtgaaag acgtttacat
    22021 caagctggaa aaagagactg acgccgggat tatcgtcagc ggtgcgaaag tggttgccac
    22081 caactcggcg ctgactcact acaacatgat tggcttcggc tcggcacaag tgatgggcga
    22141 aaacccggac ttcgcactga tgttcgttgc gccaatggat gccgatggcg tgaaattaat
    22201 ctcccgcgcc tcttatgaga tggtcgcggg tgctaccggc tcgccatacg actacccgct
    22261 ctccagccgc ttcgatgaga acgatgcgat tctggtgatg gataacgtac tgatcccatg
    22321 ggaaaacgtg ctgatctacc gcgattttga tcgctgccgt cgctggacga tggaaggcgg
    22381 tttcgcccgt atgtatccgc tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat
    22441 tacggcactg ctgaaaaaat cactcgaatg taccggcacc ctggagttcc gtggtgtgca
    22501 ggccgatctc ggtgaagtgg tggcgtggcg caacaccttc tgggcattga gtgactcgat
    22561 gtgttcagaa gcgacgccgt gggtcaacgg ggcttattta ccggatcatg ccgcactgca
    22621 aacctatcgc gtactggcac caatggccta cgcgaagatc aaaaacatta tagaacgcaa
    22681 cgttaccagt ggcctgatct acctcccttc cagtgcccgt gacctgaaca atccgcagat
    22741 cgaccagtat ctggcgaagt atgtgcgcgg ttcgaacggt atggatcacg tccagcgcat
    22801 caagatcctc aaactgatgt gggacgccat tggcagcgaa tttggtggtc gtcacgagct
    22861 gtatgaaatc aactactccg gtagccagga tgagattcgc ctgcaatgtc tgcgccaggc
    22921 acaaagctcc ggcaatatgg acaagatgat ggcgatggtt gatcgctgcc tgtcggaata
    22981 cgaccagaac ggctggactg tgccgcacct gcacaacaac gacgatatca acatgctgga
    23041 taagctgctg aaataacgca gcaggaggtt aagatgcaat tagatgaaca acgcctgcgc
    23101 tttcatgacg cgatggccag cctgtcggca gcggtaaata ttatcaccac cgagggcgac
    23161 gccggacaat gcgggattac ggcaacggct gtctgctcgg tcacggatac accaccgtcg
    23221 ctgatggtgt gcattaacgc caacagtgcg atgaacccgg tttttcaggg caacggcaag
    23281 ttgtgcgtca acgtcctcaa ccatgagcag gaactgatgg cgcgccactt cgcgggcatg
    23341 acaggcatgg cgatggaaga gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg
    23401 cagccggtgc taaaaggttc gctggccagt cttgaaggag agatccgcga tgtgcaggcg
    23461 attggcacac atctggtgta tctggtggag attaaaaaca tcatcctcag tgcagaaggt
    23521 cacggactta tttactttaa acgccgtttc catccggtga tgctggaaat ggaagctgcg
    23581 atttaagctc cagccccttc acgcattcgt cgaaggggca tttttattgt gatctcaatc
    23641 acatatctac ccgatgaata attcttgccc aaccgctcac cgccattcct gaccgcttac
    23701 atccctaaaa taaccactca gttatttacc ttactttacg cgcacgtatc tctggcaaca
    23761 tcactgcagg atagcggtca atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc
    23821 ggcctatgat taacaaatta ctctatggat tttggtttgc aggaaggcag caagtgagtg
    23881 aatccccggg agcttacaaa agtaagtgtc agaggtgaac gaacgcagct gccgcacctg
    23941 taagccaaaa gacgacgagt aaaatgccag gttttactat ggatacgaaa aaactattca
    24001 agcacatacc ctgggtgatt ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat
    24061 tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc
    24121 tggtggcgta tcgctactac agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca
    24181 cgcgcgcgac gcctgcggtt attaacaacg acggtctgaa ctacgttccg accaaccgtt
    24241 acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc tggtccgctg gtgggaccgg
    24301 ttctcgccgc gcagatgggc tacctgcctg gcacgctgtg gctgctggcg ggggtagtgc
    24361 tggccggtgc ggtacaggac tttatggtgc tgtttatctc ctctcgccgt aacggcgcat
    24421 ctctcggtga gatgatcaaa gaagagatgg ggccagtacc ggggactatc gcgctgtttg
    24481 gctgtttctt aatcatgatc atcatcctcg ccgttctggc gctgattgtg gttaaagccc
    24541 tggccgaaag tccgtggggt gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta
    24601 tgggtatcta catgcgcttt atccgtccgg ggcgtgtggg tgaagtctct gttattggta
    24661 tcgtgctgct ggttgcctct atctacttcg gtggcgtgat tgctcacgat ccgtactggg
    24721 gtccggcact gacctttaaa gacaccacca ttaccttcgc gctgattggc tatgcgtttg
    24781 tttccgcact gctgccggtg tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc
    24841 tgaaaatcgg cgttatcgtc ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga
    24901 aaatgcctgc gatgacccag tacattgacg gtactggccc gctgtggaaa ggcgctctgt
    24961 tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc tggcttccac gcgctgatct
    25021 cttccggtac gacgccgaaa ctgctggcta acgaaaccga cgcgcgtttc attggctacg
    25081 gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct ggttgcagcg tccatcatcg
    25141 aaccgggtct ttacttcgcg atgaacaccc cgcctgctgg ccttggcatc accatgccta
    25201 acctgcatga aatgggtggc gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta
    25261 ccgcacacgc ggcagcgacc gtcagctcct ggggcttcgt gatttcgcca gagcagatcc
    25321 tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccga
    25381 cgctggcggt aggtatcgct cacgtgttcc acaaagtgct gccgatggct gacatgggct
    25441 tctggtatca cttcggtatt ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg
    25501 gtacccgttc tggccgcttt atgctgcaag acctgctggg taacttcatc ccgttcctga
    25561 aaaaaaccga ctctctggtt gccggtatca tcggtactgc gggctgtgtg ggtctgtggg
    25621 gctacctgct gtatcagggc gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc
    25681 tgttcggtat ctccaaccag atgctggcag ccgtagcgct ggtactgggc accgttgtgc
    25741 tgattaagat gaagcgcacc caatacatct gggtaactgt tgttccggct gtatggctgc
    25801 ttatctgcac cacctgggcg ctgggtctga aactgttcag caccaacccg cagatggaag
    25861 gcttcttcta catggcaagc cagtacaaag agaagattgc taacggtact gacctgacgg
    25921 cgcagcagat tgccaacatg aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga
    25981 gtattctgtt cctgattgtg gtgtacagca tcatcttcta cggtttcaaa acctggcttg
    26041 cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc gtacgttcca atcccggaag
    26101 gcggcgtgaa gatctcttcg caccactaac cgtgtttagc cccgcttcgg cggggctttg
    26161 ttcttatcag agtgaactat gtttggtaac ttaggacagg caaaaaaata tctcggccag
    26221 gcggcgaaga tgttgattgg tattccagac tacgacaact acgtcgagca tatgaagacc
    26281 aaccatcccg acaagccgta catgagctat gaagaattct tccgcgaacg ccagaacgcg
    26341 cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt aatggagaga gcatgaaccc
    26401 gattgcagtt accctactca ccggtttttt aggcgcgggc aaaactaccc tgctgcgcca
    26461 tattcttaac gaacaacacg gctacaagat tgccgtgatt gaaaacgaat tcggcgaagt
    26521 ctccgttgat gatcaactga ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg
    26581 ctgcatctgc tgttcgcgct ccaacgagct ggaggacgca ctactcgacc tgctggataa
    26641 tctcgacaag ggcaatattc agttcgatcg tctggtgatt gaatgcaccg gcatggccga
    26701 tcccggcccg attattcaga cctttttctc ccatgagatt ttatgccagc tctacctgct
    26761 ggacggcgtg attgcgctgg tggatgcggt acatgccgat gagcagatga accagttcac
    26821 catcgcccag tcccaggtgg gctacgccga ccgtattctg ctgactaaaa ccgacgtcgc
    26881 aggcgaagca gagaaactgc gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta
    26941 caccgtcacc cacggcgaca tcgacctggg cctgctgttc aacaccaacg gttttatgct
    27001 ggaagagaac gtcgtcagca ccaaaccgcg tttccacttt atcgcggata aacaaaacga
    27061 tatttcgtca attgtggtgg aactggatta cccggtagat atcagcgaag tttcccgcgt
    27121 gatggaaaac ctgctgctgg aatcggcgga taaactgctg cgttacaaag ggatgctgtg
    27181 gattgacggc gaacctaacc gcctgctgtt ccagggcgtc cagcgcctct acagcgccga
    27241 ctgggacagg ccgtggggcg atgaaaaacc gcatagcaca atggtgttta tcgggattca
    27301 gttgccggag gaagagattc gggcggcgtt tgcgggattg aggaagtaag ttcgggaaag
    27361 atcaactttt tgagagttcc accgcaacat tattacgagt ggaactcttt agatatgtca
    27421 gaccaaccaa agcttcttca attgcgtttt cattttttca tgtctcattt tcagttcagc
    27481 caatccccat tctgaagaat tacaaacatc ttttgttaaa taaaaagagc ttttactatt
    27541 aagtttctca taaatagatt tctttttgat aaaatcatag tgttgtgttt cagaattttt
    27601 ggaaccactt atgagtgtta aattacctat tttatgcagc cagtagacat gctcttgagg
    27661 ttggaatcta ttaatccagt attcattaac taatgcttga ggaagaatat gctcaattgt
    27721 gattctcccc gt
//
LOCUS       DAJTUN010000044        27091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTUN010000044 DAJTUN010000000
VERSION     DAJTUN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.044"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO470_001795"
                     /pseudo
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO470_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO470_001796"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO470_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6251064.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO470_001797"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO470_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6251065.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO470_001798"
     CDS             3807..3995
                     /locus_tag="OO470_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6251066.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO470_001799"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO470_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6251067.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO470_001800"
     CDS             4756..5115
                     /locus_tag="OO470_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6251068.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO470_001801"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO470_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO470_001802"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO470_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6251069.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO470_001803"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO470_001803"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6251070.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10895..11071
                     /locus_tag="OO470_001804"
                     /pseudo
     CDS             10895..11071
                     /locus_tag="OO470_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11399..13147
                     /gene="bcsC"
                     /locus_tag="OO470_001805"
                     /pseudo
     CDS             11399..13147
                     /gene="bcsC"
                     /locus_tag="OO470_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13229..15217
                     /gene="hmsP"
                     /locus_tag="OO470_001806"
     CDS             13229..15217
                     /gene="hmsP"
                     /locus_tag="OO470_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6251071.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15402..16688
                     /gene="dctA"
                     /locus_tag="OO470_001807"
     CDS             15402..16688
                     /gene="dctA"
                     /locus_tag="OO470_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6251072.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            16909..18405
                     /locus_tag="OO470_001808"
     CDS             16909..18405
                     /locus_tag="OO470_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6251073.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OO470_001809"
     CDS             complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OO470_001809"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6251074.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19662..20429
                     /gene="pdeH"
                     /locus_tag="OO470_001810"
     CDS             19662..20429
                     /gene="pdeH"
                     /locus_tag="OO470_001810"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6251075.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20499..22559
                     /locus_tag="OO470_001811"
     CDS             20499..22559
                     /locus_tag="OO470_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6251076.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22741..24063)
                     /locus_tag="OO470_001812"
     CDS             complement(22741..24063)
                     /locus_tag="OO470_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6251077.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OO470_001813"
     CDS             complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OO470_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6251078.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25536..26435)
                     /locus_tag="OO470_001814"
     CDS             complement(25536..26435)
                     /locus_tag="OO470_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6251079.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26955..>27091
                     /locus_tag="OO470_001815"
     CDS             26955..>27091
                     /locus_tag="OO470_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR6251080.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YP"
BASE COUNT         6445 a         6855 c         7416 g         6375 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttccgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggctacaagg agaaacttgg tcccgccaag
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaataatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgtgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caactctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtagtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc aggccgatac
    11221 gttaatgcgc aatctggcgc agcagaagcc gaacaacccg gagcaggttt acgcttacgg
    11281 gctgtacctc tcaggtcaca accaggacag agcggcgctg gcgcatatca acagcctgcc
    11341 gcgcgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa gcgatcaggt
    11401 gctggaaacc gctaatcgcc tgcgagaaag cggcaaagag gctgaagcgg aagcgatgct
    11461 gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg cgtaacaacg
    11521 acgtgattac accgccgccc gcactgcata tcagaatgtc ctgacgcggg agccaactaa
    11581 cgccgatgcc attcttggtc tgacggaagt ggatattgct gccggtgaca aagcggcggc
    11641 acgtagccag ctggcgaaac tgcctgccac cgataacacc tcgctgaaca ctcagcggcg
    11701 cgtggcgctg gcacaggcgc agcttggaga taccgcagcg gcgcagcaga cgtttaataa
    11761 gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg tactgcgcga
    11821 cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa cctacaaaga
    11881 cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca cctttacccg
    11941 actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca gcgatgcggc
    12001 ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact ggggttcgag
    12061 cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg tggatgcgcc
    12121 gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg tcggcagttt
    12181 ctccactaat gccgatggca aatgggatga caactggggc acctgtacat tacaggactg
    12241 tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct ggcgaaatga
    12301 cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg atgtggtcgg
    12361 cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg cccaccgtcg
    12421 gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga gcaataccgg
    12481 gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct acgataaagg
    12541 tgaagtaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta aaaatgtcga
    12601 agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta accagaacaa
    12661 tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag atctgagtgg
    12721 ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt ttgccatacc
    12781 ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt ctggctcgtg
    12841 gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga tcccgaccga
    12901 ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg gcttcggcta
    12961 cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg gtacggcaat
    13021 tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg tacgttattc
    13081 cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac cttacgccga
    13141 ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat actcgggcgg
    13201 caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa caatcaagca
    13261 gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca ccgttttgct
    13321 gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa gcattgctcg
    13381 ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg aagcggaagc
    13441 tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa
    13501 ccagttccag gcgctgcgta aaagttttat tccagagcgt ccggtgccgg taatggttac
    13561 tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc
    13621 aaatccgcag ccaatagcct atctggtgct acaggcggat tccttccgta tgtataagtt
    13681 cgtgatgagc actctctcaa cgttagtgac catttactta cttttgtcgc ttatcctgac
    13741 cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca atattgctcg
    13801 cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat taccgcgtct
    13861 gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc agcaattgct
    13921 gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc
    13981 gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc
    14041 gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca
    14101 acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt cgccacgtat
    14161 gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg tacaggaacc
    14221 gtggcacgca atcactttgg gtcagcaagt actcactatc atgagcgagc gcctgccgat
    14281 tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct acggcgatct
    14341 caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc gccataaagg
    14401 caagaatcag attcagttct ttgatccgca gcagatggaa gcagcccaga agcggttgac
    14461 ggaagagagc gatatcctta atgcactgga aaatcatcag tttgcaattt ggttacagcc
    14521 acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc gtatccagca
    14581 accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt gctgtgggct
    14641 gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg cagcctggca
    14701 agagcgcggc attatgctgc ccttgtcggt aaacttctct gcgctgcaac tgatgcaccc
    14761 gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc cgggaacact
    14821 gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg tggcaatcct
    14881 ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca tgggctacgc
    14941 agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga aaatcgacaa
    15001 aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta tcatgctggc
    15061 gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac aacgagactg
    15121 gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgcc cactccctat
    15181 tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg attacaaaac
    15241 tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt gtttcaatta
    15301 tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc aggtaccccc
    15361 cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc tctctgttta
    15421 aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt ggccatttct
    15481 atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga
    15541 tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc atggaaagca
    15601 tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca
    15661 tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg
    15721 tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc
    15781 agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc gcatttgcca
    15841 gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc
    15901 tgggcagcaa aggccaactg atttttaacg tgatcgaaag tttctcgcag gtcatcttcg
    15961 gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gttcggggca atggcgttta
    16021 ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt atctgtttct
    16081 acattacctg tatcctgttt gtggtgctgg tattgggttc aatcgctaaa gcgactggtt
    16141 tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta ctggggactt
    16201 catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta
    16261 aatcggtggt ggggctggtt atcccgacag gctactcgtt taaccttgat ggcacatcga
    16321 tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag atggatatcg
    16381 tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa
    16441 cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat ttgccggtag
    16501 cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt gcgctaacta
    16561 acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc
    16621 acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg cacgaattat
    16681 cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg tttgcgcata
    16741 attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc acttcttccg
    16801 tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt tagactgacc
    16861 gacaacgggc agttttttta ttaccaggat agttgatcag gggttcacat gcagggcata
    16921 aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca
    16981 gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg
    17041 caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat
    17101 accggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt
    17161 gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg gcagcagggg
    17221 atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat
    17281 ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta tctggcaaat
    17341 gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca aagtcaggac
    17401 atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg tctgaaaggg
    17461 tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaaa
    17521 attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact ggtggtggga
    17581 aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga actgaaaggc
    17641 aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt
    17701 attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag
    17761 ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgcgaggcg
    17821 ctgttctggc atgttcagca agcgttaagc gccagtgaca gcaaagacat cggtcttgga
    17881 tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac
    17941 gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa
    18001 ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa
    18061 ctgtttgccg cctatgcacg ggctgatacc gatattctga tgggccagcg gatgcgttcg
    18121 ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg gcaggatttc
    18181 cttaacagcc tgactgtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat
    18241 atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag
    18301 gcggcctggg atcaaatcat ggccccatct accgccgctg cgaccacctc tgtcgccacg
    18361 gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg
    18421 agttagcaca accatcaggt atttatggat gagctggata gaggggtaac actttatcca
    18481 gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat cgcgccgcga
    18541 tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg
    18601 cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg
    18661 atcacttttt ctttcggcag tttcaccgcc ggaacatcaa ctaacgcttc accagcaatg
    18721 gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg
    18781 gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc gtcgtccagc
    18841 gtcaggaagg cgatatccgt gcattccagc atttgctggt acacctgctg tgtctcttct
    18901 ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gcctttggcg
    18961 cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg
    19021 ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac
    19081 tgctcgctcg ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt
    19141 tcgccggtgc tgtcggtttc aatgtagtaa aggcccggaa gacggttttc catccgttgg
    19201 gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatctgctg gctaaaactg
    19261 tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg
    19321 atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc
    19381 tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat agtcgtgaac
    19441 ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta
    19501 aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag
    19561 tgaggaaatt taccccggcc aggcggtaaa gttctgccct tacgcgccga taatctttgt
    19621 cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc
    19681 agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc
    19741 agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg
    19801 tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc
    19861 gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag cagattgatt
    19921 tgctggcgca aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg
    19981 atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc
    20041 ttccctggct gcgtttcgaa ctggtggagc atatccgtct gccgaaagat tcaacctttg
    20101 cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt
    20161 tctctgcact aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga
    20221 tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat ctaatgaatc
    20281 gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg cgtgatgttc
    20341 agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatggaaa
    20401 cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgccggg ctggactatc
    20461 tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa
    20521 ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa
    20581 cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc
    20641 gtccgttcgc tatccgtggc aatctgggcg tggtgtggga gcggcaaaaa caagaaactg
    20701 gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt cttggtaatc
    20761 caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca acgctggcac
    20821 cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa aagcccgacg
    20881 cgcgcctgat tcgcctctct gaaaagaaca ataactggac gtttaatctt gccaacgatg
    20941 acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat aatattcttt
    21001 tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg
    21061 ttgatccgtt aggcaagccg ctgccattca gcgaagttac tggatcgaaa ggtaaagcgg
    21121 ataaagaaaa ggtgggcgat tacatttttg gcctgaaggc gcagggacgt tataacggtg
    21181 aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc gaagggacgc
    21241 cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt gatggcgtcg
    21301 tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct ggcgattcac
    21361 tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg tttgaaacgg
    21421 atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat tatcgcggtt
    21481 ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc accggaaagc
    21541 cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac
    21601 cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga
    21661 agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg
    21721 ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca
    21781 gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc
    21841 tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt catctggaag
    21901 gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag
    21961 aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg
    22021 aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga
    22081 aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg
    22141 tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg
    22201 cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg
    22261 agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcggaacag ctggatttga
    22321 ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg ctgtatgtgc
    22381 gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt gcccgtggtg
    22441 ctgttgctgc ggcactggca acgctggtaa caccggcggc ggcgttactg gcactgatct
    22501 caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat
    22561 gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt ggcaaagtgt
    22621 gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc cttgtgaatt
    22681 caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt ttcacactta
    22741 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    22801 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    22861 gatatacggc gcaacggaag ccccgagaat cgacgatacg ttgtaagaga acgatgctcc
    22921 ggtgtaacgc acttctgtcg gaaacagctc tggtaacagc gcacccatcg gcccgaaggt
    22981 cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    23041 accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgatgatcac
    23101 catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca ccatcacgcc
    23161 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    23221 aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    23281 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    23341 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    23401 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    23461 tggcacacgc cagccccagc tcataaactg ctcatcggtc agtagccagg aaagcagcaa
    23521 gaaagtgccg ttggcaaaga agaagccaat cggtgcgcct agctgtggga aggagccata
    23581 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcac cgccccattc
    23641 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagtg gggcgaaaat
    23701 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    23761 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    23821 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    23881 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    23941 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    24001 attacgcgag ttgatcggcg tgtattcttg ttcgtggtcg agtgttgtgg ctgttgcttg
    24061 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    24121 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    24181 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    24241 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggag
    24301 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    24361 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    24421 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    24481 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    24541 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    24601 aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat cacgatttta
    24661 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    24721 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    24781 atcgccaggc caatcaatcg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    24841 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag cgtcacaatc
    24901 agcgcaatca acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    24961 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    25021 aggttaccca tccagttgat gccggaataa agtgccaccg ccaggccgac aagccctaca
    25081 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagtgtcgga
    25141 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    25201 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga caaaaatgag
    25261 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgcg
    25321 cgaataaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    25381 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt aatcggctcg
    25441 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    25501 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    25561 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    25621 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    25681 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    25741 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    25801 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    25861 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    25921 agatagtgcg gactggcaca gttgaccatg gttagtttgc cgaggggacg ggcgataacg
    25981 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    26041 accggacggt tatggctact cagttccagc tcaattcccg gatgctgata gagaaattcc
    26101 gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat gcgtaattta
    26161 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    26221 acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgta
    26281 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    26341 cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    26401 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    26461 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    26521 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    26581 atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct ttaaagtgaa
    26641 tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt tattttggct
    26701 tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    26761 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    26821 tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa
    26881 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    26941 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    27001 taaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggtc agtcaggcag
    27061 acgagttatg gcaaaagctg gaaagttacc c
//
LOCUS       DAJTUN010000045        27070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTUN010000045 DAJTUN010000000
VERSION     DAJTUN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.045"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..985)
                     /locus_tag="OO470_001816"
     CDS             complement(<1..985)
                     /locus_tag="OO470_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6251081.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFI"
     gene            complement(999..2501)
                     /locus_tag="OO470_001817"
     CDS             complement(999..2501)
                     /locus_tag="OO470_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6251082.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(2641..3597)
                     /gene="ytfQ"
                     /locus_tag="OO470_001818"
                     /pseudo
     CDS             complement(2641..3597)
                     /gene="ytfQ"
                     /locus_tag="OO470_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            3907..4437
                     /gene="ppa"
                     /locus_tag="OO470_001819"
     CDS             3907..4437
                     /gene="ppa"
                     /locus_tag="OO470_001819"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6251083.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(4816..5157)
                     /locus_tag="OO470_001820"
     CDS             complement(4816..5157)
                     /locus_tag="OO470_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6251084.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5160..8939)
                     /gene="tamB"
                     /locus_tag="OO470_001821"
     CDS             complement(5160..8939)
                     /gene="tamB"
                     /locus_tag="OO470_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6251085.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(8936..10669)
                     /gene="tamA"
                     /locus_tag="OO470_001822"
     CDS             complement(8936..10669)
                     /gene="tamA"
                     /locus_tag="OO470_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6251086.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            10875..11513
                     /gene="msrA"
                     /locus_tag="OO470_001823"
     CDS             10875..11513
                     /gene="msrA"
                     /locus_tag="OO470_001823"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6251087.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            11836..13179
                     /locus_tag="OO470_001824"
     CDS             11836..13179
                     /locus_tag="OO470_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6251088.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13241..13447)
                     /locus_tag="OO470_001825"
     CDS             complement(13241..13447)
                     /locus_tag="OO470_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6251089.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            13772..14326
                     /locus_tag="OO470_001826"
     CDS             13772..14326
                     /locus_tag="OO470_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6251090.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(14389..15327)
                     /locus_tag="OO470_001827"
     CDS             complement(14389..15327)
                     /locus_tag="OO470_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251091.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(15539..16279)
                     /gene="cysQ"
                     /locus_tag="OO470_001828"
     CDS             complement(15539..16279)
                     /gene="cysQ"
                     /locus_tag="OO470_001828"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6251092.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            16469..18412
                     /gene="cpdB"
                     /locus_tag="OO470_001829"
     CDS             16469..18412
                     /gene="cpdB"
                     /locus_tag="OO470_001829"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6251093.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(18538..18918)
                     /locus_tag="OO470_001830"
     CDS             complement(18538..18918)
                     /locus_tag="OO470_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6251094.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19007..19867
                     /gene="qorB"
                     /locus_tag="OO470_001831"
     CDS             19007..19867
                     /gene="qorB"
                     /locus_tag="OO470_001831"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6251095.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            19976..20941
                     /locus_tag="OO470_001832"
     CDS             19976..20941
                     /locus_tag="OO470_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6251096.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21049..21711
                     /gene="ytfE"
                     /locus_tag="OO470_001833"
     CDS             21049..21711
                     /gene="ytfE"
                     /locus_tag="OO470_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6251097.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(21756..23168)
                     /gene="cycA"
                     /locus_tag="OO470_001834"
                     /gene_synonym="dagA"
     CDS             complement(21756..23168)
                     /gene="cycA"
                     /locus_tag="OO470_001834"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6251098.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(23476..24096)
                     /gene="fklB"
                     /locus_tag="OO470_001835"
     CDS             complement(23476..24096)
                     /gene="fklB"
                     /locus_tag="OO470_001835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6251099.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            24315..24860
                     /locus_tag="OO470_001836"
     CDS             24315..24860
                     /locus_tag="OO470_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6251100.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(24844..25491)
                     /locus_tag="OO470_001837"
     CDS             complement(24844..25491)
                     /locus_tag="OO470_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6251101.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            25703..26494
                     /locus_tag="OO470_001838"
     CDS             25703..26494
                     /locus_tag="OO470_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6251102.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            26504..>27070
                     /locus_tag="OO470_001839"
     CDS             26504..>27070
                     /locus_tag="OO470_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6251103.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHV"
BASE COUNT         6601 a         7189 c         6674 g         6606 t
ORIGIN      
        1 tgataaagcg ttgcgactga acaatcagca cacaaagcac caccaccgct ttcaccacct
       61 ggttcatctc cggcggaaag cccgaaagca aaattccggt gttcatcccc tgaataatca
      121 gcgcccccac taccgagagc agcaggttaa agcgcccgcc catcagcgat ccgccgccaa
      181 tcaccaccgc gagaatggcg tccagctcca gccataatcc ggcgttgttg gcatcggcac
      241 cgcgaatatc cgccgccacg ataatgcccg caatcgccgc acatagcccg ctcaacacgt
      301 aggtgagcat gacgatgatt cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga
      361 taccaacgac ttcgataaac atccccagcg ccgttttgcg ggtcaacagc cagaacagga
      421 gaagcgtcag caccgcgata atgaccggcg ttggcaggaa caacagcgat ccgctgccaa
      481 accatgagag atccggcgag ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca
      541 cgccgcgccc ggcgaccatc aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg
      601 ctaccagtat gccgttccac aatcccgcca ggatgccggt gcccagggcg cttaacaaaa
      661 caatcggcag gctgaatccc gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca
      721 tcaccgcccc cacagagaga tcgataccac cggtggcgat caccagcgtc ataccaatcg
      781 ccagtaacgc aacgggagct gcacggttaa gaatgtcgat agggctaccg aacaaacgcc
      841 catcctgaag caccacctgc cagaaatgcg gggctaccag gctatcgacc agcagcacca
      901 gcaacagtgc cgccagctgc ggcatccccg tcggccagtg aaaacgcctt tttggcggcg
      961 tagtgtcagg gagagattga ggcatcacac tgttctcctt acgccgcaat ggcgttcatg
     1021 atcgccggaa cggaaagctg tgccagcggg atctccgcca cctgtttgcg atcgcgcatg
     1081 atgatcaccc ggtcggcata gcccaccagc tcttccagtt cggaggagat caccagcagc
     1141 gccagaccgt cagcacacag cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca
     1201 tcaatgccac gggttagctc atcaaggatc agaaattgcg gtcgggtcag tagccaacgt
     1261 gaaagcagca ctttttgctg attgccaccg gagagaaatt caatcggttg ttcagttgaa
     1321 ggtgtgcgaa tgccaagctg gcggataaag cgttcggcaa tctcttgctg ctctttgcgg
     1381 gaaatgggtc gtagccagcc gcgctgggcc tggagagcga ggatgatatt ttcccgcacc
     1441 gaggcggcgg cgatgatgcc atcggttttc ctgtcttccg ggcagaatcc gatgcccagt
     1501 actgaagcct gatgtggcga tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg
     1561 ctgtcagcag gtttgatacc gaagatcact tcggcggttt cggtacgtcc ggatcccagc
     1621 aagccagcca gaccgacgat ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt
     1681 ccttttttgc cgtaattttt gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg
     1741 gcacgctgta gcgcgtgggt atccagctcg cgccccagca tcatttttac cagttcgatc
     1801 tgcggtagct cgcgcgtttc ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc
     1861 cgatcgctga cctgatagac ctgatcgaga aagtgagtga caaagatcag gctgacgccg
     1921 cgatcgcgca actgacgcat caggccaaat aatagctcca tttcctgggt gtcgaggctg
     1981 gcggtgggtt catcgaggat cagcactttg gcggagagat cgatagcccg gcaaatggcg
     2041 acgatttgct gcatcgccac cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa
     2101 ccgtaagatg ccatcagttc ggtggcgcgc ttttccatct ctttacggcg tagaaggccg
     2161 aaacgtttgg gttcgcgacc tatgaataga ttatcagcaa ccgacatatt gggtagcagg
     2221 ttgacttcct gatagacggt gccgataccg agttgttgtg catgggcggt atttttcggt
     2281 gagatagtct ggccttccag ccagatggtg ccgcgatcgg cgtggtatac accagttaat
     2341 gctttgatta gcgttgattt tcccgccccg ttttcaccaa gcagcgccat gatttcgcca
     2401 cggcgcaggc tgaaatcaac gttgtctaac gctttgacgc cggggaaaaa tttacttaat
     2461 ccttcggtgc ggaggatctc ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag
     2521 cgtgctccca gtaggcatga taagacgcgg taagcgtcgc atcaggcttc agtgcaccat
     2581 cgccggatgc ggcgcgagcg ccttatccgg cctacgaatc ggcatcaggc atagcaaccc
     2641 tcaatacccc atatttttct tcttctctaa ctcttctttt gcggtatcag gcaggtaaag
     2701 ggtggatttg gttaacgtca gcttttcagg catggtgccg tcttttttgt atttctccag
     2761 cgcatcgaaa gcgggtcccg ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc
     2821 atccatcatc gctttgtaga tgtccggtac accgtcaatg gaacccgtga ggatatcttt
     2881 gcccggtttc aggcccgctt ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt
     2941 atgggcgtaa accatgcaga tgtttttgct gttgttttcc gctttgataa agctctccat
     3001 gacttctttg cctttactgc gggtgaagtc acctgactgc gagcggataa ttttgatatt
     3061 tggcgcattc ttaatggctt cggcaaagcc tttcttacgg tcaatggcga cgctggcccc
     3121 aacggtgccc tgcagctcca ccacgttgca tggtttgcca ttcacttctt ttaccagcca
     3181 gtcaccgatc aacttacctt cgaggatgtt gtcggcagta acggtggtca tatagagaga
     3241 tttgtctttc acatcgatgg aacggtcgag caagaagacc gggatttcgg catctttcgc
     3301 ctcttttaat accggttccc aacccgtcgc aaccactgga gcgataaaga tcgcatccac
     3361 cccttatgca acgaaggaac gtaccgcttt aatctggttt tcctgctttt gctgaccatc
     3421 ggcaattttc aacgtaattc cgcgtttttc ggcctcactt ttcgccacat tggtttctgc
     3481 ggcgcgccag ccggattccg atccgacctg cgaaaatcct acggttaatg gagcggccaa
     3541 cgccatagac gacatggctg ccgagactgc agagactaca agtaagcgtt tccacatagg
     3601 gttgtcctcg tcggggtaat tattattggt gaaaagatgt tcgcgaaaaa actatagaca
     3661 attcgttatg taacggattg cgttacatca cacttcaaaa aagtaaataa aacgttaatc
     3721 acaagtttgt aatcgctttc atctcactat gaaaaatgcg gctacggtta tggattttcc
     3781 tgctctgtat accgtcttaa aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag
     3841 acattcggcg cgagttggct ataatacttg gcacttgttt gccacatatt tttaaaggaa
     3901 acagacatga gcttactcaa cgtccctgcg ggtaaagatc tgccggaaga catctacgtt
     3961 gttattgaga tcccggctaa cgcagatccg atcaaatacg aaatcgacaa agagagcggc
     4021 gcactgttcg ttgaccgctt catgtccacc gcgatgttct atccgtgcaa ctacggttac
     4081 atcaaccaca ccctgtctct ggacggtgac ccggttgacg tactggtccc gactccgtac
     4141 ccgctgcagc ctggttctgt gatccgttgc cgtccggttg ccgttctgaa aatgaccgac
     4201 gaagccggtg aagatgcgaa actggttgcg gttccgcaca gcaagctgag caaagaatac
     4261 gatcacatta aagacgtaaa cgatctgcct gaactgctga aagcgcaaat cgctcacttc
     4321 ttcgagcact acaaagacct cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac
     4381 gcagaagccg ctaaagctga aatcgttgct tccttcgagc gcgcaaagaa taaataagtt
     4441 cttctagcgc aataaccctg aacgccgggc ttcggttagt aagggttttt ttatgcccgc
     4501 gataaataaa ctctctattc caccatcatt attctcagcg gttgcaaggc ttgaacggta
     4561 agaacaagca aacccgacca ccattttgct gttcatagcc acttgctgga agttagccga
     4621 cctcactcat actcaccgtt caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg
     4681 ccagcagagt tttttatgcg attgcagata tggaaagcgg ctcggagaga aaagcaaaag
     4741 atggaaaagt tacaaaaaaa atcgcgagat acatcgcata aaacaacgcc acccgaagga
     4801 ggcgtgcttt tattattact tatccctgtc taaccagtcg ccgctttcaa ttagctttaa
     4861 tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccatacggcg tctgaatcaa
     4921 ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca agttcggcca gcgtggcgtt
     4981 gtcaatacga taaacttcac cgtgtaccgt tccgttcccc ggaactgcgc ctggatagtg
     5041 gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact gggcattggt
     5101 catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata ttcgcattgc
     5161 taaaactcga actgatagag caaatccagt gcctggtcta caccagacac ggcttccaga
     5221 tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc aaatataccc
     5281 acgccgtatt tcacttgcag acctggcaat acatagccgc tgaccactac ctgggaggag
     5341 tcgcctactc ccagggtgtc gagcgctaaa ttgcttacgc caaacgtctc gccgatttta
     5401 cccacaatct ggccactttg cgcaaccccc agaccaatca gcatcgaggt cattgccgca
     5461 ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa tgcagcttgt
     5521 tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag accagtgacg
     5581 cgaactccgg cgattacatc gtcttctgta gcatccgggt tacgaatagc ttcaatatta
     5641 agatacggtt gatccggcgg accagagaac agtaactcac ccttacgcac aatcagatcc
     5701 tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag gcccagccct
     5761 tgtttgtcct gtacgacgtt aagatcgccc gtcagccgcg ctttcaggcc aaaggcgtca
     5821 atgcgcacat tgttgccaac gtggacaatc aggttactgt taatcggaat cgacgccgtt
     5881 ttcggctctt ccggttgcag gttatcgtta agcatcacca catcgctgga gacgcctact
     5941 gcgctttccg gcagatcgtg caccacgatg cgcgcccacg ggacatccac gcgaccatcg
     6001 agggtaaaca ggtttggtgt agcctcgaat acaacatctg gcgatacatc cattcgtacc
     6061 atcggcggca cggtgatccg cactttactg cctttcgccg ttactcgtgc ccgccagttt
     6121 tcaatttggc tccagtcggc attaccgttc aggtagattt caccctgctg ggtccgtact
     6181 gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg caagctggct cggctgcata
     6241 tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg accaaacaac
     6301 tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc cgctgctttt
     6361 tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat attgacgttg
     6421 ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc aaactggccg
     6481 ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa ttccgccgtc
     6541 agattcagtg tctgaaacgc caccggcagc gccgcatcgt tgacggtttg cgttacctgc
     6601 acgttacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttcgt ggtgtcccag
     6661 gcaacatccg ctttacccgt gaagataccg ctggcctgag tggtttctgg cataaacggt
     6721 ttcagcatgg cgaggtcgaa gcggttgaga ttcaccaccg cacgcccttc ggcccccgca
     6781 tcgatagttt gcggcacgca cagttccgca ttcgggttaa gccagcagtg tggcccgatg
     6841 ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag cgaccacggg
     6901 ccgaccggcg tctggaagcg ggtattgcta agagttccct tccagcgttc ttctttgcga
     6961 tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat ccgcaactgt
     7021 agctcgtgct gcttttcgct gcctttggca ttcagggtga cgaggttgat atttacatcc
     7081 ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc ggtggattta
     7141 atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag gccgcgcgcg
     7201 gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac cagccctttc
     7261 gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat ggtggcatca
     7321 agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg cccgagttcc
     7381 agatgaagcc cagggatcat ccactgcata taactgttgc ctttcagcgt gccgtcaacg
     7441 ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc catctgccag
     7501 gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga cggccactcc
     7561 gggaactctt tggcggtgtt aatgccatta agcgttagct caccgcgcca actaatggcc
     7621 tgctgccagt cgagcaacgc cttgagttca gttttccctt ccagcgccgc gacggtgagt
     7681 ttgtcgagat tgacctgctg ttcattacct ttggcatcaa gggtaatggt ggctggcggg
     7741 atctcctgtc ccttcactgc cgtacgcata gagagcgtgt aatcggtcat tttgccggta
     7801 agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg ccagtaaagc
     7861 tgtttgctgt tcacttccac gttgagtggc aatccggctt ccgccagtcg cgtctgggcg
     7921 cgtaaatcca tatccaccgg accggaaagg ttaacgccta tctccagctg ttcgcgcagc
     7981 gcgccgccca ctttcagctt caccttttca cctttcaacg gctccacgtt cagtgtgctg
     8041 ttgagggtga tatccaccgg ccagttgtct gacagctgcg ccgtgccgct ggcgttgacg
     8101 atcccctggt tggaatcgat atccagggcg tccagtttag tattgccgtc aatgctgctc
     8161 actttcagca gcatggtgcg cacggtgatg tccgtgtcgc ccgtcacgcg cagctgctcg
     8221 cccttaaact cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat ttccggcaat
     8281 accgggcgag aaaagagatc tttcagcgtt tcgccgagcg gcttttcctc cggctgcgga
     8341 ttttcaattt tcggttcgac cacttcttcc tgcgccactt ccaccacttt cggcagagca
     8401 atcagcaggc ctttcagcga cgtcggtttc agggtcaggg ttttctcctg ccagttcagg
     8461 ccggaggtga agtccatcac cgacaccgtg gtgtcatcaa tcttgatgtt gacgttgtcc
     8521 agcgccaccc gtgtcagggt gatgggatac ggcgtggaga gatccagcgg accgctatct
     8581 tcctcttctt caacctgttc agaaggaggc atttttttac tgtcgatgtt gacctgaatg
     8641 tctttcagcg ccaggtcatt aatacaaacg ctactgttcc acaggcactc aagaccgacc
     8701 cccagatgca gattacccgc ttttaccgcc acgcctagct gctcataacg aacgtcagac
     8761 aaggtgagat cgcgccagcc gccggtgacc ttgccaatat ccagtcctgg cacccagcga
     8821 tccgccgctt taaatacaag atgcaggccg ctggtggtgc ccaccagaaa cgccaccgat
     8881 cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca taaactcata
     8941 attctggccc cagaccgatg taaaactgta acccgtgttc gtctttatcc gcgaccggta
     9001 cggcaaaatc gagtttgatt ggcccgaccg gcgattccca gcgtacgccg accccggtac
     9061 cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg acaaacaccg
     9121 cgccccacca ttttccggtc acgttgtact ggtattccag cgatccggtt atcaacttcg
     9181 aggccccttt caggtcaccg ttggcgtatt tcggggcgat agatttgtat ttgtagccgc
     9241 gaatactgcg atcgcccccg acgaagaaac gcagatccgg cggtactttg tcgaaatcac
     9301 cggtttcaat ccagcccagc gtgccgcgtg tcacaaagcg atggcgatcg tacagtgtgc
     9361 ggatccagac gtgctgcgcc tggaaaacgg agaaatcgac atctgaaccc caggccgtgt
     9421 tggagtagtc gatagagtag cgttgcgagt ctccccaggt tggcatcagg ccaccacgag
     9481 aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg gtaatttcac
     9541 cctgggtaaa gtggtcgagg ctccagcgca ggttaatggc acgctgccag ccgctggaga
     9601 gatcccagta gcgagaagcc accaacgtag tggagtcaga ttcggtatcg ttcaggtcag
     9661 tgcgcttaaa accgccctgc accaaataat attgttccag tggattcttc agcagcggca
     9721 ttttatagct gaagtcgagg gtctgttccg gcgcggaaat actggtactg gtggtcaaac
     9781 tgtgaccgta tgagttcatc cacggctttt tccacgtcgc tttcacgcgc ggtcccacgt
     9841 ccgtagagta accgaccccg gtttcgatgg tgttttctgt tcgcggcgaa accacgcccg
     9901 tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc accaccgagt
     9961 taaaccagcc ggtagcagaa agtcggcggt tcagttccgc cagatctttc gattcatact
    10021 catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt tgtgatcctt
    10081 caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata tcccagaagg
    10141 ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta tcgaaataac
    10201 ctttacgcaa cgcaatactg gttaaggact ttttgaaatt ttcataatcg ccctggttca
    10261 gtaccgtacc aatagccggg cgagtatcga gcaatttcaa atagtcttta tcggtccgcg
    10321 cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacacct ggcgtgactt
    10381 tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat tcaatggtcg
    10441 gctggtaata acccagcgct ttcagacctt cacggatggc atcatcgacg cgtgcgcgaa
    10501 agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgca cgaacgttct
    10561 tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg acggcagatc
    10621 cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca ttttctcctg
    10681 aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa tccagtaata
    10741 tggattaaaa agcagactaa accccaaata tttcttatgt tttactttag acctattcac
    10801 ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt gaccccaatg accgattttc
    10861 gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc gccgatgccc
    10921 tgcctggacg taacaccccg atgcccgtag ccacgctgca tgcggtcaac ggtcactcaa
    10981 tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt ttctggggtg
    11041 tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc tataccggcg
    11101 gctatacgcc aaatccgact tatcgggaag tgtgctccgg tgatacgggt catgccgaag
    11161 cggtacgcat tgtttacgat ccttccgtca tcagctatga gcagttgcta caggtatttt
    11221 gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc acgcagtatc
    11281 gttcagcgat ttatccgctg accccagaac aggatgccgc agctcgcgcc agtctggaac
    11341 gttttcaggc ggcgatgctt gccgccgatg acgatcgtca catcaccacg gaaatcgcta
    11401 acgccacacc gttttattat gccgaagatg accaccagca atatctgcat aaaaacccat
    11461 atggttactg tggaattggc ggaattggcg tctgtctgcc gccggaagca tagcgttacg
    11521 ggtacaaatg tagtttgttg ataaagtgct ctttgtctat gcctgatgcg gcttaaacga
    11581 cttaccaggc ctacaaaatc gcccaaattc aatatattgc aaggactgcg taggcctgat
    11641 cggcatagcg catcaggcag tattgcattt atcagcagtc tgaattttaa ccctctggcg
    11701 actttacagt accttacgct atactagcca ctgaaaatgc cggttcactt tcttcgaatc
    11761 ggctttcaat gtgtatttca cacaaattaa tcaactttcc cttccgaggg tctggcctga
    11821 aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgcttgat cgctgtaagt
    11881 gcgttcttct cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa acttaaactg
    11941 ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga aaatcccggc
    12001 atgttcttta ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg cggtatcgtc
    12061 ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat gtcggcagag
    12121 ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat gtttatcctg
    12181 tttgcggatt taaccccgaa acgcatcggt atgattgcgc cagaagcggt ggctttgcgt
    12241 atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg gttcttcaac
    12301 ggcctggcga acatgatctt ccgtattttc aaactgccaa tggtacgtaa agatgacatc
    12361 acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt gttacgtaaa
    12421 caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt tccgtcttca
    12481 atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca aagcctgaag
    12541 aataaggtgg cggaacatcc gcactctaag ttcctcgtct gtaatgaaga tattgaccac
    12601 atcatcggtt atgtcgattc taaagacctg ctgaaccgcg tgctggctaa ccaaagcctg
    12661 gcactgaaca gcggcgtaca aattcgcaac acgctgattg tgccggatac gttaaccctt
    12721 tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat catgaacgag
    12781 tacgcgctgg tggtggggat catcaccctc aacgacgtga tgaccacgct gatgggcgat
    12841 ctggtcggtc aggggctgga agagcagatt gtcgcccgtg atgagaactc atggctgatt
    12901 gacggcggca ccccaattga cgacgtcatg cgcgtgctgg atattgacga gttcccgcag
    12961 tcgggcaact acgaaaccat cggtggcttt atgatgttta tgctgcgtaa gatcccgaaa
    13021 cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat cgataactac
    13081 cgcatcgacc agctgctggt gacccggatc gacagcaagg ccaccgccct ttcgccaaaa
    13141 ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac atcaacggct
    13201 cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc tctgtttgca
    13261 gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta tccttcactt
    13321 ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aatgtccttg atgtataacc
    13381 gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac cgttggaaaa
    13441 ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc ggcccaacta
    13501 ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat gtttccaccg
    13561 cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt accgaagcgc
    13621 ggtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc aaactggcat
    13681 cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc caagtggtcg
    13741 gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc tggcactcac
    13801 ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaatcggtc agcgagtgcc
    13861 gccgattggc attaccgatc ggggcgagtt ggtgcttgat aaagatcagt ttagctacaa
    13921 aacctggaac agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata ttgctggtcg
    13981 cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag cgaagttacc
    14041 gcacgatcgt taccagacca ccaccattgt taacaccgac gacgcgattc cgggttccgg
    14101 catgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt cgcagtttat
    14161 tgtcgatagc aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa gttccgctgt
    14221 agtggtgctg gataaagacg gtcgcgtgca atgggccaaa gacggggcgc tcacaccgga
    14281 agaggtgcag caagtgatgg acctgctgca aaagttgctc aaataatggc aataacataa
    14341 attctggaaa aataattttc ccctatcgtt atctatggga taggggaatt aagtctctga
    14401 gttcttacac cgaactaata ctggtaacgc tagttgctct tttttaaacg cctcaatggt
    14461 cgatttatat ttctcgcaaa tcttttcttt ggtaatatta ctatctgcgg ccagattcgc
    14521 acttactatc gcatccactt gttcggctaa ttcgacttgt tggcaacctg cattaaccag
    14581 aaaattataa ataagagagt aatcttcatc ctccaaaaca acagaggaac cattacgaaa
    14641 acgttcatcc cctccataga ttttaagcgc aaagcccaaa gattctatga ctatggcaaa
    14701 attatcatca attatttttt cgtaaatata ccatccatcg cattctttaa ttccaccgcc
    14761 tggtatttgg agttcaaatt taccccgttt taatttgctt actacatcct ccaataaggc
    14821 ataaccgaaa gatacgtcac caaacgcctg gcgaatataa cctttaatcc tttttggtat
    14881 gtcagactgt tttttctgca aaatatcaca ggcatacaat cttctgtttt gttctaacgg
    14941 aaactcgcgg tttttgaaat ccatgaatga gttatactca tttattgtta tgggaatttc
    15001 ttcgtattca aacccactgc ctcgcaatgt tattgtatat tcagcaccgt tatctttaag
    15061 gctgaaactg ataagaaaat tatccatggc attaacagtt tcattttttg atgaaagcac
    15121 ttgataacta tttttatcaa tctgtgttgc gcaacgaaag gactcccgca aagttaacag
    15181 actatcataa ttcgaaaccc aatctttttt cttaaatgca tgctgaatat tattaaaaaa
    15241 atcgtttgaa tgtgtttttg tatgcgatga ctttacaaat aactccaccg caccaataaa
    15301 attattgtgt ctgatcctgg gtaacatatg gctaccttat tttatttggt tattttatgt
    15361 caccattaaa tatctcatta tagaagtaaa ctatcaaaaa acgctcatta ttaaaatgta
    15421 acttactagc aattgaattt ttgcacttta aatacaatct ctttttattt ataacttttt
    15481 aggttgggat aaatcaggat ggcagaatca ggaaatacac tgtttctgcc atctgaattt
    15541 agtaaatgga cactctgaac cccggattca ggaacgactc gcgcggagtg taatccagcg
    15601 gtttaccctg ccagtcgtga acgtgcgctc cggcagctgc agccacagca tgtccggcgg
    15661 cggtgtccca aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca
    15721 ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg atgttcgcca agctgttgca
    15781 gatactcttt cagctccgca tctgcatggg aacggctgat aaccaccagc ggcgggcgcg
    15841 catcgcggac ctgaatcagc ttgcgcacac cgcactcttc tttccaggcc ttgccttctg
    15901 ccgcgctgta cattacgttc attaccggcg catacaccac acctaaaatc ggtttgccat
    15961 ggtcaatgag cgcaatgtta acggtgaatt cgccattacg tttaataaac tctttagtac
    16021 catccagcgg gtctaccagc cagtaacgct gccagtgctg acggacttcc caaccgggag
    16081 gatcttcttc agaaaggacc ggaatatccg gtgccagcgt acgtaaaccg tccatgataa
    16141 cggtgtgagc ggcaatatcc gctgccgtta ccggagaatt gtccgctttg ctgacgacgt
    16201 ccatcggttt cgtcccgtcg tagacctgca taatggcatc gcctgcattc cgtgcaagct
    16261 ggcatacttg atctaacatt tctccacctc gtctctgtga gcggtgttaa cttattgttt
    16321 tacttatacc ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg
    16381 taaaattgtg gcattcttca ctgttctata agtaaggcgt ttattctccc ttttctttcg
    16441 tattcccgat gataaaagga tgtccctgat gattaagttt agcgcaacgc tcctggccac
    16501 gctgattgcc gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg aaaccactga
    16561 tctgcatagc aacatgatgg atttcgatta ttacaaagac accgccacgg aaaaattcgg
    16621 actggtacgt acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa acagcgtact
    16681 ggtcgataac ggcgatttga ttcaggggag tccgctggcc gattacatgt cggcgaaagg
    16741 attaaaagca ggtgatgttc atccggttta taaggcgctg aatacgctgg attatacggt
    16801 cggtacactc ggcaatcatg aatttaacta cggtctggat tacctgaaaa atgcgctggc
    16861 gggagcgaaa ttcccttatg taaatgccaa cgtcattgac gccagaacca aacagccaat
    16921 gtttacaccg tatttaatta aagataccga agtggtcgat aaagacggaa aaaaacagac
    16981 gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg ataaagctaa
    17041 tttatccgga aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga
    17101 aatgcgcgag aaaggtgccg atgtcattgt cgttctggcg cattccgggc tgtctgccga
    17161 tccgtataaa gtaatggcgg aaaactcagt ttattacctc agtgaaattc cgggcgttaa
    17221 cgccattatg tttggccatg ctcacgccgt tttcccgggt aaagattttg ctgatatcga
    17281 aggggctgat atcaccaaag gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg
    17341 gggcgatcat cttggggtgg tcgacttaca actcagtaat gacagcggta aatggcaggt
    17401 gacgcaggcg aaagcggaag cacggccgat ttacgacatc gccaataaaa aatccctcgc
    17461 ggcggaagac agcaagctgg tagaaacact caaagccgat cacgatgcca cacgccagtt
    17521 cgtcagcaag ccaatcggta aatctgccga caatatgtat agctatctgg cgctggtgca
    17581 ggacgatccg accgtgcaag tcgtgaacaa cgcgcaaaaa gcgtatgtcg aacattacat
    17641 tcagggcgat ccggatctgg caaaactgcc ggtgctttca gctgccgcac cgtttaaagt
    17701 tggtggtcgc aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt
    17761 ccgtaatgct gccgatcttt atctctaccc caatacgctg attgtagtga aagccagcgg
    17821 taaagaggtg aaagagtggc tggagtgctc cgccggacag tttaaccaga ttgctcctaa
    17881 cagcacgaaa ccacagtcac tcatcaactg ggatggtttc cgcacttata actttgatgt
    17941 tattgatggt gtgaattatc agattgatgt tacccaaccc gcccgctatg acggcgagtg
    18001 ccagatgatt aatgccaatg cagagcggat taagaacctg acctttaatg gcaagccgat
    18061 tgatccgaac gctatgttcc tggttgccac caataactat cgcgcttacg gcggcaaatt
    18121 tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc gctcggtgct
    18181 ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa attcatccgg cggcagataa
    18241 caactggcgt ttagcaccga tagctgccga taagaaactg gatatccgtt tcgaaacttc
    18301 cccgtcagat aaagccgcag cgtttattaa agagaaaggg cagtatccga tgaataaagt
    18361 cgcgaccgat gatatcgggt ttgcgattta tcaggtggat ttgagtaagt aaaacacttc
    18421 tttttcggcc tataaatcaa ccgcatccgg catttattgg cggatgcgat gctgccgtat
    18481 cttatccacc ctacaagcca tgaaccgtag accggataag cgcagcgcat ccggcagtta
    18541 tgccgcacgt tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa tccagtccgc
    18601 cagtgcagca accttttcgc tcacctgctc gcccagcggc gtgaggctat attccacatg
    18661 cggcggcacc accggatacg cgatacggtt aagaaaacca tcctgttcta acgcctgtaa
    18721 cgactgcgca agcatctttt cactcacccc accgattttg cgccgcaggt cgctaaagcg
    18781 atgagtacct tcgcgtagcg ccaccagaat caacaccccc caacggctgg tgacgtgttt
    18841 caacacctcg cgcgacgggc actgttccgc aaagagatta ccctctttca gttgttgtga
    18901 caggctaacc tgactcattt catacttacc tttttgcacg tacttactaa aagtaagttt
    18961 aggtgttagc gtatttaaac acaagacaaa acgatggaga cttcccatga tcgctattac
    19021 tggtgccact ggccaacttg gtcactatat tattgaatcc ttgatgaaaa cggttcctgc
    19081 cagccaaata gtggctatcg ttcgtaatcc ggcaaaagcc caggccctgg cagcacaagg
    19141 cattaccgtg cgtcaggctg actacagcga tgaagccgca ctgacatctg cacttcaggg
    19201 agtggaaaaa ctactgctga tctcttccag cgaagtgggt caacgtgccc cgcagcatcg
    19261 taatgttatt aatgccgcaa aggcggctgg cgtgaaattt atcgcttata ccagcctgct
    19321 acatgcagat acctccccgc tcggcctcgc cgatgagcac atcgagacgg agaaaatgct
    19381 ggctgattct ggcatcgttt acaccctgct gcgcaacggc tggtacagcg aaaactacct
    19441 cgccagcgcc ccggcagcac tggaacacgg cgtatttatc ggtgcggcgg gcgatggcaa
    19501 aatcgcctca gcaacgcggg cagattatgc gacagctgcg gcacgcgtga ttagcgaagc
    19561 cggtcacgaa ggcaaggttt acgaactggc gggcgatagt gcctggacgt tgacacagtt
    19621 agcggcagag ctgaccaaac agagcggcaa acaggttacc tatcaaaatc tgagcgaagc
    19681 cgatttcgcc gcggcactga aaagcgtcgg actgcccgac ggactggcgg atatgctggc
    19741 ggattctgac gttggcgcat cgaaaggtgg tctgtttgat aacagcaaaa cgcttagcaa
    19801 attgattggc tgcccaacga caacgttagc cgaaagcgta agccatcttt ttaatgttaa
    19861 taactagtta attaaagtgg catcctcccg catcctctct gataatgacg ggatgccggg
    19921 agcaatcatg tctgcttcct gaacgtttct tctgacagac caatggatgc cagtaatgat
    19981 tagcggcgtg ctgtacgccc tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc
    20041 gttgatcgtg ccggaatacc cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg
    20101 gttaattgcg ctacccattg cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg
    20161 ggactggctt accgccttga tgctcactat gatgggcaac ctcatttatt acttctgcct
    20221 tgccagtgcc attcaacgta ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc
    20281 ggtggtgatt cctgtctttg ccaatctgct ttatagccag cgcgacggca aactcgcgtg
    20341 gggaaaactc gccccggcac tgatttgtat tggcatcggc ctggcgagtg tgaatattgc
    20401 tgagttaaac cacggactcc ccgattttga ctgggcacgt tatacctctg gcatcgtgct
    20461 agcgttagtt tccgtggtct gctgggcatg gtatgccctg cgcaacgccc gctggctgcg
    20521 ggaaaatccc gacaaacatc cgatgatgtg ggcgacggtg caggcgctgg tcacgctgcc
    20581 ggtttctctc atcggctatc tcgtcgcctg ttactggctg aatatacaaa cgccggactt
    20641 ctccttacct tttggccccc gtccgctggt gtttattagt ctgatggttg cgatagccgt
    20701 gctttgctca tgggttggcg cactctgctg gaacgtcgcc agccagcgat taccgacagt
    20761 gattctcggg ccgctgattg ttttcgaaac gctggcaggt ttgctgtaca cctttttact
    20821 ccgccagcaa atgccgccgc taatgacgct gagcggtatc gcgctgttag tgattggcgt
    20881 ggtcatcgcg gtcagagcaa aaccagaaaa acctttaact gaatctgtct cagaaagttg
    20941 acatgctggc agtgagttaa ataagcctct gctacgtaag ggttatagct tttgccttaa
    21001 aggtgtattt aaaatacatc ttatcttatt aagaatgagg tatcagctat ggcttatcgc
    21061 gaccaacctt taggtgaact ggcgctctct atccctcgcg cttcggctct gtttcgtaaa
    21121 tatgatatgg attactgctg tggcggtaag cagacgctgg cgcgcgcggc ggcacgtaaa
    21181 gaactggatg ttgaggtcat tgaagctgaa ctggcaaagc ttgctgaaca accgattgag
    21241 aaagactggc gtagcgcccc gctggcagaa atcattgacc atatcatcgt gcgctaccac
    21301 gatcgtcacc gcgagcagct gccggagctg atcctgcaag cgactaaagt cgagcgcgtt
    21361 cacgccgaca aaccgagcgt gccaaaaggg ctgacaaaat acctgaccat gctgcatgaa
    21421 gagctttcca gccacatgat gaaagaagag cagatcctct tcccgatgat caaacaaggc
    21481 atgggcagcc aggcaatggg gccaatcagc gtaatggaaa gcgagcacga tgaagcgggc
    21541 gaactgctgg aagtgatcaa acacactacc aataacgtca caccgccgcc agaagcctgc
    21601 accacctgga aagcgatgta taacggcatt aatgaactga ttgatgacct gatggatcac
    21661 atcagtctgg aaaacaatgt actgttccca cgcgccctag cgggtgagtg atctaaaagc
    21721 tggatggcat tgcgccatcc agcatgataa tgcggttatt tccgcagttc agcagcccgc
    21781 ttcttaccaa taaacagcca gcccaacccc agcgcgataa accacagcgg ggtaaccagc
    21841 agcgcctggc gagtgtcatc ttccagtgtc agcaacacga ccacgaacac aaagaacgcc
    21901 atacataccc agcacatcag cttgccgagc ggcatcttgt agatcgactt ttcatgcaga
    21961 tgaggacgct gtttgcggta caccaggtac gagcaaagga taatcgtcca gacgaacata
    22021 aacagaatcg cggaaacggt tgtaatcatc gtgaacgcgc caatcacgct aggattcaca
    22081 tacaacatca ccacgccgcc gagcagacag atacacgaga aggtcaaccc tttcgcgggt
    22141 actgcgcgct tagaaagttt agcgaacgct ttcggcgcaa caccttcctg cgccagacca
    22201 aacagcatac ggctggtaga gaagacaccg ctgttagcgg aagacgccgc agaggtcagc
    22261 accacaaagt tgatcacgct ggcagccgca ggcaaaccta ccaacacaaa cagctcaacg
    22321 aacgggcttt tctccgggac taccgaactc cacggtgtca cggacataat cacaatcagc
    22381 gcgaagacgt agaacataat gatacggatc ggaatggagt taatcgcgcg tggcagtgat
    22441 ttctccggat ctttggtttc cgcagctgtt gtacctacca gctcaatccc cacgaaagcg
    22501 aaaaccgcta tctggaatcc ggcaaagaag ccacttaaac ctttcgggaa ccagccgccg
    22561 tcattccaca aatgcgcgaa tgacgcttcc acaccggtcg gtgactgaaa gtgcatcgcc
    22621 accatgacca ggccgacgac aatcagcgac acgatggcga cgattttgat catcgcaaac
    22681 cagaactcca tctcaccgaa cattttcacg gtggcaaggt tgagcgtcag cagcagcact
    22741 atcaccgcca gcgaggcgac ccagtcggag agatcgggga accagaactg agcataagcc
    22801 gtgatcgcca ccacgtctgc cataccggtt acaacccagc agaaccagta agtccagccg
    22861 gtgaaatatc ccgcccacgg ccccagtaaa tcggaagcga agtcactaaa agatttgtat
    22921 tccagattcg aaagcagcaa ttcccccatt gcccgcatca cgaaaaagag cataaaacca
    22981 atgatcatat aaacgaaaat gatcgacggc ccggcaaggc taatcgtttt gccggacccc
    23041 ataaacaacc cagtaccaat ggcacctcca atggcaataa gctgaacatg tcggtttgtg
    23101 agattgcgcc gtagcgactg ttcagccgga gcctgatcat cggcaacgac ttttacctga
    23161 tctaccatgt tttttcttcc tgtacctgtc tgtgttgttc aggctctacg gcctttagtc
    23221 agtctatgat acgacgaatc tgtatcggga ctcatcgata ttaggtaaca atacgcggga
    23281 tgaatactaa gatttagatc taatgttaat tttatgttta aagtgagtgt catatcacgg
    23341 tgataacgcg aaacagctca caaaaataca cgcttacggg gtctttgcaa cacaaaatag
    23401 cgccgtttgg caaactgaag ggtttattgc tgaatgcctg ctcccctctg gttcgagggg
    23461 aacagaatgc gctgcttaga ggatttccag cagttcgact tcaaacacca gggtgctgaa
    23521 cggagggatg gatgcacctg cgccgcgctc gccatatgcc agttcctgcg ggatagtcag
    23581 ttcccattta gaacctaccg gcatcagagt cagtgcttca atccagccag ggatcacgcc
    23641 attaaccggg aattcagcgg gttcaccacg agcaacggag ctgtcaaaca cggtgccgtc
    23701 gatcagttta ccggtgtaat gaacacgaac gcggtcggta cgtgccggaa ttgcgccttc
    23761 accctggttg atcacgcgga attgcaggcc agattcggtg ctattcacac cttctttttt
    23821 ggcgttttct tccaggtatt tcacgccttc agcagccatc gcctggaaac gctgacgacg
    23881 aacggcatcg gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat caaccggcac
    23941 agccggatgt ttgccttcca gcgcatcggc aatacctgca accagtgctt ctggcagcag
    24001 cccttccagg ccagattcac tcagttgttg ccctacctgc aaaccaatgc cgtagcttgc
    24061 ttgcgcttcg atggtgtcaa aagttggggt ggtcatcgta tttcctttct tagggtataa
    24121 aagtagcggg cagcatatca gccacacttc tttgggtaaa actttgtcgc aggaatgatg
    24181 acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat aaaggcgggt
    24241 ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa caagacgtgg
    24301 agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag aaagtctggc
    24361 acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt cgggggatca
    24421 ttattgccgc cattgtattg gtggtcggat ttctgcttcc atctgatgat acgcccaacg
    24481 cgtcggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa ccgccgacag
    24541 aagaacagcc gcaaactacc caaacgcaac cattccagcc agatagcgga attgataatc
    24601 aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc cgcgaccacg
    24661 gactgcccgc taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt aagccgctga
    24721 gtaatttgca aaatgggcag atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg
    24781 gcttaaccat tgatacgggg aataatcaac aggtcctgtt tacccgccag ccggatggca
    24841 gttttattcg tgcgcggtag atgaagggtc aaccaaaagc cagctaagga ttgtctcttt
    24901 gatgacctta cgtctggctt ccagtgcctg gggggaaacc atactgatat gataaactag
    24961 gccaagcgta tgctggttga tcaaataata tcccccaagc gcagcaatat taatattcac
    25021 ctgcaatggg tcgatacctg gcttaaaaat attgtatttc ttcccttcat ccaataagga
    25081 ttccattaac tgcaagtgag catggttaat ttctaagaga cgctgtgatt ttgcataatg
    25141 cacaccttta ctttgatttt cactatgcac aatttttaaa aaccaaggat tagcaatata
    25201 ataatcccat gtaaaatcaa taattttctc aatggctttt tgtggcggaa gatctgagaa
    25261 atcaagggcc ttttcttttt gtcgaatatc gttccaggta tattccagca cttcgataaa
    25321 taaattttct ttattaccaa agtggtgata aaccgtctgt ttactacacc ctgctgcctt
    25381 tacaatatta tctactctgg ccccttcata accatattcg gcaaattcat taaccgcaca
    25441 tagcagcaat ttttccttca ggctctgtac gttgctggta tcaatataca taacgcttcc
    25501 ccacgacctc ttcgtggtga ctatcataac ctgatttaat ttcagcttca gaaaaaaggg
    25561 caaaaacttg tactttcatc aggttatcaa atgtcctgtg atcgccatcg catatcaacc
    25621 aactagatgg ttgatctttt tatcctccag tcgcaagcta atcatcacca aggaaacaaa
    25681 attcaaaaaa gagagctatt gtatgaaatc cactcgtcct gttgccgtga ttaccggagc
    25741 cactcgcggt attggaaagg gctgtgcgca ggagcttgct cgtggcgggt ttaacctgct
    25801 gattaacgat cgtcccgatg ccgacagcgt agaaaaactg catatcacac aacaagaatg
    25861 tttggcggaa ggcgtggaag tgatttgctt ccctgctgat gtcggtgatc tatctctgca
    25921 tgaggagatg ctcgacgcgg cgcaaaacca gtgggggcgg ctggactgtc tgctcaataa
    25981 cgcggggatt tcagtgaaaa aacgcggtga cctcctcgac ctcgaaccag acagcttcga
    26041 tcaaaacatt gccattaaca cccgcgcgcc gttcttcctt gctcaggcat tcagtaagcg
    26101 cttactggca cagccgaaac cggaagcgga gctgccgcac cgctcgatta ttttcgtcag
    26161 ttccatcaac gccatcatgc tggcgatgaa tcgcggcgaa tacaccattg ccaaaaccgc
    26221 cgtttctgcc gctgctcgtc tgtttgctgc tcgcctttgt aatgaacaaa ttggcgttta
    26281 tgaagtgcgc cctggtctca tcaaaactga tatgactatt ccggcaacgg cttattacga
    26341 cgaactgatt gccaaaggtc tggtgccgtg gggacgttgg ggttatccgg ctgatattgc
    26401 ttccaccgtc cgcgcgatgg cggaaggtaa actgatttac acctgtggtc aggcggtcgc
    26461 aatcgacggc ggcttaagta tgccgcgctt ctgaggctga atcatgagag atctgttaca
    26521 acgcccggat ttattcagta tcaataccgc gacactgggc tataaaacgc cgctacccgc
    26581 cattattgat gcctgcgccg cacgcggtat tggcgcaatt gccccctggc gcagggagtt
    26641 gcagggtgaa gatctccagc aaatcacccg ccagcttgcc gccagtaaca tgagcgtttc
    26701 cggtttgtgc cgtagcactt actacaccgc gccgacactg gcggaacgca aactggcgat
    26761 tgatgataac cgccgggcgc tggacgacgc agcggtgctt aacgccgcct gttatatgca
    26821 ggtggttggc ggtctgccaa cgggcactaa agatctatat gaagcgcgag aacaagtgaa
    26881 acaagggatt cgccagttgc tgccacattc gaaagatgtc ggcgtgccaa ttgcgctgga
    26941 gccactgcac ccgatgacag ctgccgatcg ctcttgcctg tgcacgttac gtcaggcgct
    27001 ggactggtgt gatgaactcg atcccgatgg tgagtttggc ctcggcgtgg cggtggatgt
    27061 ttatcacgtc
//
LOCUS       DAJTUN010000046        27001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTUN010000046 DAJTUN010000000
VERSION     DAJTUN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.046"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO470_001840"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO470_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6251104.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO470_001841"
     CDS             complement(1614..1937)
                     /locus_tag="OO470_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6251105.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO470_001842"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO470_001842"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6251106.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO470_001843"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO470_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6251107.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO470_001844"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO470_001844"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6251108.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO470_001845"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO470_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6251109.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO470_001846"
     CDS             7537..8574
                     /locus_tag="OO470_001846"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6251110.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO470_001847"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO470_001847"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6251111.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO470_001848"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO470_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6251112.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO470_001849"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO470_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6251113.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO470_001850"
     CDS             10745..11626
                     /locus_tag="OO470_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6251114.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO470_001851"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO470_001851"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6251115.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO470_001852"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO470_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6251116.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO470_001853"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO470_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO470_001854"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO470_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6251117.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO470_001855"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO470_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6251118.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO470_001856"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO470_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6251119.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO470_001857"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO470_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6251120.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO470_001858"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO470_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6251121.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO470_001859"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO470_001859"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6251122.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO470_001860"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO470_001860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6251123.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO470_001861"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO470_001861"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6251124.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO470_001862"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO470_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6251125.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO470_001863"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO470_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6251126.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO470_001864"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO470_001864"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6251127.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO470_001865"
     CDS             24893..25273
                     /locus_tag="OO470_001865"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6251128.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO470_001866"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO470_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6251129.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO470_001867"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO470_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6251130.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27001
                     /locus_tag="OO470_001868"
                     /pseudo
     CDS             26442..27001
                     /locus_tag="OO470_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6672 a         6715 c         7083 g         6531 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 c
//
LOCUS       DAJTUN010000047        26449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTUN010000047 DAJTUN010000000
VERSION     DAJTUN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.047"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            348..641
                     /locus_tag="OO470_001869"
     CDS             348..641
                     /locus_tag="OO470_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6251131.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="OO470_001870"
     CDS             713..1372
                     /locus_tag="OO470_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6251132.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="OO470_001871"
     CDS             1464..1910
                     /locus_tag="OO470_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6251133.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO470_001872"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="OO470_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="OO470_001873"
     CDS             3390..3809
                     /locus_tag="OO470_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6251134.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="OO470_001874"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="OO470_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6251135.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO470_001875"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="OO470_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6251136.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO470_001876"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="OO470_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6251137.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="OO470_001877"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="OO470_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6251138.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="OO470_001878"
     CDS             complement(8332..9864)
                     /locus_tag="OO470_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6251139.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="OO470_001879"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="OO470_001879"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6251140.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="OO470_001880"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="OO470_001880"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6251141.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="OO470_001881"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="OO470_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="OO470_001882"
     CDS             complement(14765..16501)
                     /locus_tag="OO470_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6251142.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO470_001883"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="OO470_001883"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6251143.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="OO470_001884"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="OO470_001884"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6251144.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="OO470_001885"
     CDS             complement(18223..18957)
                     /locus_tag="OO470_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6251145.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="OO470_001886"
     CDS             19158..19412
                     /locus_tag="OO470_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6251146.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..20922)
                     /locus_tag="OO470_001887"
     CDS             complement(19462..20922)
                     /locus_tag="OO470_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6251147.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20859..21416)
                     /locus_tag="OO470_001888"
     CDS             complement(20859..21416)
                     /locus_tag="OO470_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6251148.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21442..22296)
                     /locus_tag="OO470_001889"
     CDS             complement(21442..22296)
                     /locus_tag="OO470_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6251149.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="OO470_001890"
     CDS             complement(22296..23105)
                     /locus_tag="OO470_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6251150.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="OO470_001891"
     CDS             complement(23115..23873)
                     /locus_tag="OO470_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6251151.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="OO470_001892"
     CDS             complement(23870..24850)
                     /locus_tag="OO470_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6251152.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="OO470_001893"
     CDS             complement(24850..25872)
                     /locus_tag="OO470_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6251153.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6647 a         6454 c         6825 g         6523 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgtga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa taggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagaatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacacgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcact cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttaggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt actcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgctggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt tttccctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gagcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattaccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgga
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 caccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggagaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatga
//
LOCUS       DAJTUN010000048        26332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTUN010000048 DAJTUN010000000
VERSION     DAJTUN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.048"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..836)
                     /locus_tag="OO470_001894"
     CDS             complement(<1..836)
                     /locus_tag="OO470_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR6251154.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LR"
     gene            complement(1074..2294)
                     /gene="fsr"
                     /locus_tag="OO470_001895"
     CDS             complement(1074..2294)
                     /gene="fsr"
                     /locus_tag="OO470_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6251155.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            2512..4164
                     /gene="ushA"
                     /locus_tag="OO470_001896"
     CDS             2512..4164
                     /gene="ushA"
                     /locus_tag="OO470_001896"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6251156.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(4201..4680)
                     /gene="ybaK"
                     /locus_tag="OO470_001897"
     CDS             complement(4201..4680)
                     /gene="ybaK"
                     /locus_tag="OO470_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6251157.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(4884..5678)
                     /locus_tag="OO470_001898"
     CDS             complement(4884..5678)
                     /locus_tag="OO470_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6251158.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            5816..6157
                     /locus_tag="OO470_001899"
     CDS             5816..6157
                     /locus_tag="OO470_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6251159.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(6372..8876)
                     /gene="copA"
                     /locus_tag="OO470_001900"
     CDS             complement(6372..8876)
                     /gene="copA"
                     /locus_tag="OO470_001900"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6251160.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            9138..10070
                     /gene="glsA"
                     /locus_tag="OO470_001901"
     CDS             9138..10070
                     /gene="glsA"
                     /locus_tag="OO470_001901"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6251161.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            10073..11365
                     /locus_tag="OO470_001902"
     CDS             10073..11365
                     /locus_tag="OO470_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6251162.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            11490..11924
                     /gene="cueR"
                     /locus_tag="OO470_001903"
     CDS             11490..11924
                     /gene="cueR"
                     /locus_tag="OO470_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6251163.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(11898..12356)
                     /locus_tag="OO470_001904"
     CDS             complement(11898..12356)
                     /locus_tag="OO470_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6251164.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(12353..13270)
                     /locus_tag="OO470_001905"
     CDS             complement(12353..13270)
                     /locus_tag="OO470_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6251165.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            13416..14093
                     /gene="fetA"
                     /locus_tag="OO470_001906"
     CDS             13416..14093
                     /gene="fetA"
                     /locus_tag="OO470_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6251166.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            14080..14859
                     /gene="fetB"
                     /locus_tag="OO470_001907"
     CDS             14080..14859
                     /gene="fetB"
                     /locus_tag="OO470_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6251167.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(14922..15776)
                     /gene="cnoX"
                     /locus_tag="OO470_001908"
     CDS             complement(14922..15776)
                     /gene="cnoX"
                     /locus_tag="OO470_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6251168.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(15837..16646)
                     /gene="ybbO"
                     /locus_tag="OO470_001909"
     CDS             complement(15837..16646)
                     /gene="ybbO"
                     /locus_tag="OO470_001909"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6251169.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(16636..17258)
                     /gene="tesA"
                     /locus_tag="OO470_001910"
                     /pseudo
     CDS             complement(16636..17258)
                     /gene="tesA"
                     /locus_tag="OO470_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            17229..17915
                     /locus_tag="OO470_001911"
     CDS             17229..17915
                     /locus_tag="OO470_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6251170.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            17912..20327
                     /locus_tag="OO470_001912"
                     /pseudo
     CDS             17912..20327
                     /locus_tag="OO470_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(21039..22133)
                     /gene="mnmH"
                     /locus_tag="OO470_001913"
     CDS             complement(21039..22133)
                     /gene="mnmH"
                     /locus_tag="OO470_001913"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6251171.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(22202..23128)
                     /gene="allS"
                     /locus_tag="OO470_001914"
     CDS             complement(22202..23128)
                     /gene="allS"
                     /locus_tag="OO470_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6251172.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            23358..23840
                     /gene="allA"
                     /locus_tag="OO470_001915"
     CDS             23358..23840
                     /gene="allA"
                     /locus_tag="OO470_001915"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6251173.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            23918..24733
                     /gene="allR"
                     /locus_tag="OO470_001916"
     CDS             23918..24733
                     /gene="allR"
                     /locus_tag="OO470_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6251174.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            24823..>26332
                     /gene="gcl"
                     /locus_tag="OO470_001917"
     CDS             24823..>26332
                     /gene="gcl"
                     /locus_tag="OO470_001917"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6251175.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSE"
BASE COUNT         6169 a         6814 c         7079 g         6270 t
ORIGIN      
        1 tcgcgcaatg gcaacgtatc gtgggcggca cggtgactca attcagactc gttcagtacc
       61 atcccggcaa agaacgcacc gagtgcaaag gagacatcaa acagctctac cgcaccaaag
      121 gcaatcccta acgccagcgc cagcactgac agggtaaaca gctcgcgaga accggttgcc
      181 gcgctgcgtg ccataatcca cggcaccaga cggcgaccta ccagcatcat aatggcgata
      241 aatgcgatca ccttgccgat ggtgatcccc atatcgactg caagagtggc aaagcccaca
      301 tcgccctgtt ccatcattcc tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc
      361 aggtcttcca caatcaacca accgatggcg atttgcccac gctgactgtc tattaattgc
      421 cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg tggaaagaca taagccgaac
      481 acgataccgg tcattaacga ccagcccagc acggcggaga gcgccatacc cagcagcgtc
      541 gccacggcta tctgggcgat cgcgccggga atggcgatgg actttaccgc catcaaatcc
      601 ttgagcgaaa aatgcaaacc gacgccgaac atcagcaaaa taacgcccag ttcagccagt
      661 tccggcgcaa gcttggtatc ggcaacaaag cccggagtga atggtcctgc cagcacaccc
      721 gctaacagat atcccaccag aggagaaata cgtagtttat tggccagcat gccgaggata
      781 aaggcgagca caaggccgcc aacaatggtg gtgataagcg gggtggcgtg atgcattccg
      841 tctccttttc ctggtggtta ttgtccattt ttggctggga aaaccaaaat tacaggtaat
      901 agtttatgac aatttcattg atgatgttta tgaataattg ttgaattttg cagaaaaatg
      961 gaattagctg caaaaaaagc acggttcgga aaacagaagg cggtttaaca gaggaaaggc
     1021 ttatggcgac tgtaaaacga tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct
     1081 ttatgccggt tatcaggcag gaatatggtc aacatcccca atagtggcag gaaagcacag
     1141 attttataga ctaactcgat gctggtgtga tcggcgataa gccccagaac tgccgctccc
     1201 agacctccca tgccgaaagc aaaaccgaaa aagagtccag aaaccatacc gatacgtccc
     1261 ggaagtagct cctgagcgta gaccagaatg gcagagaatg ccgaagcgag gataaatcca
     1321 ataatcaccg ttaaaacccc cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac
     1381 ggcgcaacgc cgaggataga gccccaaatc acatatttcc gtccaatttt atcccctaca
     1441 ggcccgccga tcaccgtacc tgccgcaacg gcaaacagga aggcaaacag atgaagctgg
     1501 gcattctgga tagataatcc gaatttttgc atcagataaa aggtgtaata gctgctgatg
     1561 ctcgccatat agaaatattt cgagaaaatg aggattaaca gaatgctgac tgccagtacc
     1621 actttgttgc gcggcagtgg attgattatc gtcgctttgg gttttccttt attcattcgg
     1681 tgctgtgccg agtaccaacg gctaatttgc gccaacacca cgatcgccag cagtgccgca
     1741 agcacaaacc aggcaacgtt gcctttgcca taaggcgcga taatcaccgc cgccagcaag
     1801 ggtcccaggg aactgccaaa gttgccgccg acctgaaaga tagattgcgc caggccatgc
     1861 cgcccgccgg aagccatacg ggctacgcga gaagattccg gatgaaagac cgatgaaccg
     1921 gtaccgacca gcgccgctgc cagcagaact gcgccaaaac tgcccgccag cgcaagcagc
     1981 accagaccac ttaaggtaaa gcacatgcca attggcaacg accacggcat cggatattta
     2041 tcggtccagt agccgaccac tggttgcagt agcgaagagg cgagctggaa ggtgagggtt
     2101 atcatgccaa tctgcataaa tgtcagagaa aattctgact gaagcagcgg ataaatcgcc
     2161 agaatcagcg attggatcat gtcgttcagc agatgtgaga ggctgatagc acctaaaata
     2221 ccaaacgatg ttcgggcctt ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt
     2281 tcactcattg ccataggaaa gtcacttttt cagggttgcg atgtaaagaa tgatcttatt
     2341 tgtgattatt acccgactaa catacctgta tgcgtcgtct gaaggaagtc tcaacgccga
     2401 atacagaatt tctaatctgg atgcagattt atcttcaccg gacgcagact tgtctatgat
     2461 gtcgcgtcat actatttttc aacacgttga aatcaggtca gggagagaag tatgaaatta
     2521 ttgcagcggg gcgtggcgtt agcgctgtta accacattta cactggcgag tgaaactgct
     2581 ctggcgtatg agcaggataa aacctacaaa attacagttc tgcataccaa tgatcatcat
     2641 gggcattttt ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg
     2701 gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact ttccggtggc
     2761 gacattaaca ctggcgtgcc cgagtctgac ttacaggatg ccgaacctga ttttcgcggt
     2821 atgaatctgg tgggctatga cgcgatggcg atcggtaatc atgaatttga taatccgctc
     2881 accgtattac gccagcagga aaagtgggcc aagttcccgt tgctttccgc gaatatctac
     2941 cagaaaagta ctggcgagcg cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg
     3001 aaaattgccg ttattgggct gacaaccgat gacacagcaa aaattggtaa cctggaatac
     3061 ttcactgata tcgaatttcg taagcccgcc gatgaagcga agctggtgat tcaggagctg
     3121 caacagacag aaaagccaga cattattatc gcggcgaccc atatggggca ttacgataat
     3181 ggtgagcacg gctctaacgc accgggcgat gtggagatgg cacgcgcgct gcctgccgga
     3241 tcgctggcga tgatcgtcgg tggtcactcg caagatccgg tctgcatggc ggcagaaaac
     3301 aaaaaacagg tcgattacgt gccgggtacg ccatgcaaac cagatcaaca aaacggcatc
     3361 tggattgtgc aggcgcatga gtggggcaaa tacgtgggac gggctgattt tgagtttcgt
     3421 aatggcgaaa tgaaaatggt taactaccag ctgattccgg tgaacctgaa gaagaaagtg
     3481 acctgggaag acgggaaaag cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag
     3541 caaatgatct cgctgttatc accgttccag aacaaaggca aagcgcagct ggaagtgaaa
     3601 ataggcaaaa ccaatggtcg tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc
     3661 aatatggggc ggttgattct ggcagcccaa atggatcgca ctggtgccga ctttgcggtg
     3721 atgagcggag gcggaattcg tgattctatc gaagcaggcg atatcagcta taaaaacgtg
     3781 ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg acatgaccgg taaagaggtg
     3841 attgattacc tgaccgccgt cgcgcagatg aagccagatt caggtgccta cccgcaattt
     3901 gccaacgtta gctttgtggc gaaagacggc aaactgaacg accttaaaat caaaggcgaa
     3961 ccggtcgatc cggcgaaaac ttaccgtatg gcgacattaa acttcaatgc caccggcggt
     4021 gatggatatc cgcgccttga taacaaaccg ggctatgtga ataccggctt tattgatgcc
     4081 gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg
     4141 aaaggtgagg tgagctggca gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc
     4201 ttaatcgcgg cgggcgatat cagcaaattt ggcatcgagg atctttgcca gatcgcctgc
     4261 cgccagttcg atatccagtc cgcgcttgcc gccggaaaca taaatagtgg caaattcttg
     4321 tgcgggggcg tcgataatcg ttggcagacg ttttttctgc cccagtgggc taatcccccc
     4381 aaccaggtat cccgtcgaac gctgcgcgac catcggatcg gccatctcaa ctttcttggc
     4441 acccagcgct tttgctactt ttttaagatc cagttgacct gcgaccggcg taacggccac
     4501 ggcaaggtgt ttcatatcac cgttcactgc caccagcagc gttttgtaga cctgatccgg
     4561 attcaaacct aattttttga cgacttcatc gccaaaattg gtttcagccg gatcgtgctc
     4621 gtaggtatgg atttgaaacg aaatcttgtt tttttcgagt aatttaactg cgggtgtcat
     4681 ggtaattttc ctgccttaaa caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa
     4741 aaaaagagtc atcttgccta agagtattgg caggatggtg agattgagcg acaatcgagt
     4801 tacaccgtcg cttaaagtga cggcataata ataaaaaaat gaaattcctc tttgacgggc
     4861 caatagcgat attggccatt tttttagcgc aacatttgcg gcaaattccc ttctccatac
     4921 aggtgtagtg caccgaccgc gaccacatat cgccccggcg gcatggcgcg taatttatcc
     4981 cgccaggcga gatttcgctg atgcatcagc acatcgtaca gcgactgact gaacgtattg
     5041 ggcagcgtta tatcattatt ttgcggcggt gcattcagcc accagctcat catttgttgc
     5101 agcaaccgtg cgttggtatg ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg
     5161 tcagggagct ggagcaacat ggcaatctgg ttttcagccc cttccagttc aatcacgggt
     5221 ttatgttgtt gcttcgccgc ctgcaatagc tggtaatcga taccgtattc tgcccgcagc
     5281 cccagttttt gtgcctgcgt cgcctgaaga accatcgcga tttgccacag cggttgggta
     5341 gaaaagagtg agggagaaat gcccatctcc tggctaatgt gctgcaggtt ttggagttgc
     5401 tcctcgctaa tgcgctcttc cagcgcctcg caggcaggca aattagcaaa aggcgtatcg
     5461 ctggtggaaa catccgcctc gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa
     5521 cgggtgggca ggggagccat atcgtggcta cccatatgaa tactgccaat cagatgaaaa
     5581 tggcgattgc cggggagggt gatatcgatg gcaggccagg tgtaatgatt accgcgcagc
     5641 gcggcccaaa gcgtttttac ccggtacaac agatccatac gacctccctt tgtgaaatat
     5701 catgctagcg cgcggtgagg gatggcgcaa atggagttgg ggacttgttt aatgtgtttg
     5761 tatgattcag tatgttcttg catcgctatt cacagggaag caacagttaa aaaccatgaa
     5821 acaggcaaca agaaaaccga cgacacccgg agatattctt ctctatgaat atctggaacc
     5881 gctcgatttg aaaatcaatg agttagcaga gttgctgcat gttcatcgta atcgcgtcag
     5941 tgcactgatc aataacaatc gtaaactcac cactgagatg gcattccgtc tggcgaaagt
     6001 ttttgatacc acagtcgatt ttaggctaaa cctccaggcg gcagttgatc tttgggaagt
     6061 tgaaaacaac atgcgcaccc aggaagaatt gggacggatt gaaacagtgg ctgaatattt
     6121 ggcacgccgt gaagagcgtg caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg
     6181 taggccggat aaggcgttca cgccgcatcc ggcatccgag caccattgcc tgatgcgacg
     6241 cttacgcgtc ttatcaggcc tacaaacctg tgcctgaacc gtaggccgga taaggcgttt
     6301 acgccgcatc cggcagtcat gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg
     6361 caatgacgta cttattcctt cggtttaaac cgcagcaacc ggttggcgtt acttactacg
     6421 gtaattgacg agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagta
     6481 aacggccaca aaataccggc ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg
     6541 agcaggttct gcttcatgtt gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg
     6601 cccatcaggc tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg
     6661 ccacccatcg caatgccgac atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg
     6721 cccaccattg ccacctgacg tccttcactt tgcagacgtt tgatcgcttc ggctttaccg
     6781 tccggcagca ctccggcaat cacttcatca attccagctt ctttggcgat cgcattggcg
     6841 gtggttgggt tatccccggt caacatcacc agacgatatc ccgctttatg caggcgttgc
     6901 agcgccgcca cgctatcact acgcaacgga tcgcgtacag ccagcagggc tactgctttc
     6961 ccgtcaatcg ccagcagcac aggcgttgct ccttgcgatg cctgagctga aatatcggct
     7021 tcaatcgctt tagtatcgac ttgctgatca tttaacaacg cctgattacc cagcaataac
     7081 gcatgacctt ctgcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc
     7141 tgcggtagct gcatatcgcc tgctttatca aggatcgctc gtgccaatgg atggctggaa
     7201 ccttgctcca gcgccgccgc cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt
     7261 ttcactgcga caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacacgacg
     7321 gtatcgagtg tactggcgcg ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca
     7381 gccgcccgcc cgacgccgga aataatcgac atcggcgtcg ccagtcccag cgcacacggg
     7441 caggcgataa tcagcaccgt agtggcaatc accagggtat agacaatctg cggtgccgga
     7501 ccaaagaaat accagattgc cgcactgaca agcgcaataa ccactactac cggcacaaat
     7561 acggctgaga ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc
     7621 accatgcgaa tgatccttga tagcgtggta tggctgccaa ccgcactggc acgaaacagc
     7681 acactgccgt cctgtaccac tgtcccggca tggacgctat cgccttcgcc tttttgctgc
     7741 gggattggtt cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg
     7801 ccatctaccg gcacgcgatc gccggtcgtc aggcgcagca acatacctgg ctgcacttct
     7861 gccagaggca cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa
     7921 tcgagtaatt tctccagtgc cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg
     7981 ccgagattga tcagaccgat aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc
     8041 atcgggaacc actgcggcca caggttgacg ctcatcgaat agagccacgc cacgcccgtt
     8101 cccagcgcca ccagcgtatc catcgtcgct gcaccgttca gcaggctttt ccatgcactg
     8161 cggtaaaaat ggccgctggc gaaaaccatc accgccaggg ttatcaggcc gataaccaac
     8221 cacaggctgc ggttgtcggc ggtgaccatc atgttatcgc cgatcatccc ccagaccatc
     8281 accgggatac ccaccgccag tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg
     8341 acggcggttt cttgctggcg ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg
     8401 tagcccgctt tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc
     8461 gcagtacgct ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca
     8521 ttttgtacgc gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg
     8581 tcatcgtcat cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac
     8641 gggattgatg actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc
     8701 gcttgtttga tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca
     8761 gtgatagaca catccgcttg ttccacatcc gggcgctgtt caagactttc tttcacgcgt
     8821 ttaacgcagt gaccgcagga caggccgtcc aggttcaggt cgatagtttg tgacataaaa
     8881 cactccttta agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa
     8941 ggtcaagaaa ttaataaacc aggcgggtaa aagtccgtaa agattaaaaa atcggctcga
     9001 tttgcatcag gattagacat ttatttcttt gttttcctgt agttaagttg cgggtgctaa
     9061 gttaaatcag gatgcctgaa aatcggcact ggggtgagga attacctccc gcatctataa
     9121 aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt
     9181 acacccaatt tcactcactt aacggcggac aaaatgccga ttacattccc tttctggcga
     9241 atgtaccagg tcaactggct gcagtggcta tcgtgacctg cgatggcaac gtctatagtg
     9301 cgggtgacag tgattaccgc tttgcactgg aatccatctc taaagtctgt acgttagccc
     9361 ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg
     9421 gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaaccg ctttcgccac
     9481 tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaac gccgaacaac
     9541 gctggcagcg aattttacat atccaacagc aactggctgg tgagcaggta gcgctctctg
     9601 acgaagtcaa ccagtcggaa caaacaacca acttccataa tcgggccatt gcctggctgc
     9661 tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc
     9721 agtgctctac gctcatcaat actgttgaac tggcaacgct tggcgcgacg ctggcggcgg
     9781 gtggtgtgaa tccgttgacg cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc
     9841 tggccgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg
     9901 gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg
     9961 gaattgccgc gttctcacca ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa
    10021 tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa
    10081 cacggaaggt aataacggta acaaacccct cggtctatgg aacgtcgttt ccatcggtat
    10141 tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg caggctgcgt tgctaatgga
    10201 agcctcgacc tggggcgcct ttgcttttgg cggtattgtg gctatgtttt ccggttatgc
    10261 ctatgcgcgt ctgggggcga gctatcccag caatggcggt attatcgact tttttcgtcg
    10321 cggattaggc aacggcgtct tttcgctagc gctctcgttg ctatacctgt tgacgctggc
    10381 ggtgagcatc gccatggttg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga
    10441 aggcagccag gaggagcacc ttattttgct ctacgcgttg gggatcattg cggtgatgac
    10501 gcttttcaac tccttaagca accatgcggt agggcggctg gaagtgatcc tcgtcggcat
    10561 taaaatgatg atcctgttat tgctgattat tgccggtgtc tggtcgctgc aaccggcgca
    10621 tatttccgtc tctgcgcccc ccagctccgg tgcgttcttc tcctgtattg ggataacctt
    10681 ccttgcctat gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgca
    10741 ggtcattatg ccacgggcgt ttctggtggc gattggcgtt accacgttgc tttatatctc
    10801 gctggcgctg gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgctgatac
    10861 cgccgtagcg caggctgctt ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat
    10921 cggcgcttta ctggcgacgg cttcaggcat taacgcgaac ctgttcgccg tgtttaacat
    10981 catggacaac atgggcagcg aacgcgaact gccgaagcta atgaataaat ccctgtggcg
    11041 gcagagtacc tggggcaata tcatcgtcgt ggtgttgata atgctgatga cggcggcgct
    11101 aaatttagac tcactcgcca gcgttgccag cgccaccttt ttgatctgct atctggcggt
    11161 gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt
    11221 tggtacgttg gtgatgttgt tggtgatcgt tggctttatc tacagcctgt ggtcccaggg
    11281 cagccgtgcg ttgatatgga ttattggtgc aatcttactc agccttattg tggcaatggt
    11341 catgaagcgt aataaaaccg tataacacct ctctgtgcgc agtacttcct gtattattgt
    11401 ggtggcggtc gatattcgca ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt
    11461 aacaaagcac aggaggcgtt gcgcgaacga tgaacatcag cgatgtagca aaaattaccg
    11521 gcctgaccag caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc
    11581 gcagcgaaaa cggttatcgc acctacacgc agcagcatct caacgaactg accttactgc
    11641 gccaggcacg gcaggtgggc tttaacctgg aagagagcgg cgagctggtg aatctgttta
    11701 acgacccgca gcggcacagc gccgacgtca aacggcgcac gctggagaag gtggcggaga
    11761 tcgaacgaca cattgaggag ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg
    11821 cctgccctgg cgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc
    11881 atcatcgggc agggggatta agacgagacg gctcggatat gtagggttat cccttctatc
    11941 gcaatgactt caacatgcgt acctgcaccg agatcctcgc tggcgctgac aggccatgaa
    12001 ctgtcaccga cgcgcatatg accgcgcccg ttgaccagcg gagattccag cacaaaacgt
    12061 cggccaatca gctgctgccc gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc
    12121 acccgccgcg acaaccattt ccaccacagc caggcggcga gcagcgtcag gatggcaaac
    12181 attacccctt gccactccca acccagcggc accagccaga ccaccaggcc ggtaatcacc
    12241 gctgccacgc cgctccacaa caaataacca tttccgccca gcatctcggc tgccagcagc
    12301 aacccgccga gactgagcca gaaaatatgt ggatgaacga ccattaactc catcatggct
    12361 gagtccgttt gttggcgctg tctttcacca gctcggcaat cccggcaatc gaccccatca
    12421 ggctgctggc ctctaatggc atcatcacta ctttgctgtt actggaggaa ccgatctgct
    12481 gtaacgcttc ggtgtatttt tgcgctacga agtagttcac cgcctgaata tcaccggagg
    12541 cgatagcttc agacaccatt ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg
    12601 cttcagcctg taaaaacgcc gactgacgtt cgccttccgc tttcaggatt tgcgactgtt
    12661 tttcaccttc ggctttgagg atttccgcct gacggatccc ttccgcttca agaatgtaag
    12721 cgcgtttggt acgttccgct ttcatctgcg cgttcattga agagataagc tctgccggtg
    12781 ggcgcacgtc gcgaatttca atacgggtga ctttaatccc ccacgggttg gtggcttcat
    12841 cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt
    12901 ccattgaacc caacacggta cggatgttag tcatggtcag gttgatgatc gccagctcca
    12961 gattgctgac ttcataggca gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt
    13021 cgatggtaac gttggcgtta tctttcgaga taacttcctg ggaagggata tcgagcactt
    13081 gctccatcat attgatcttg cgaccaatgc gatccataaa cggcaccacc agactgagcc
    13141 ccggctgtaa cgttttggta tagcgaccaa aacgttctac cgtccattga tagccctgcg
    13201 gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac aaaaatgaga atcgggataa
    13261 agataagcat cggaaaaacc tcctgttgta ccgtccataa tcagcaaaat tgctgcttga
    13321 ttaaacaaat tatacctgat tactgaaaga gagttccccc ttattcctgc gaaggataaa
    13381 ctgtttttag taaaaatcag aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc
    13441 agctacaaaa cgtaggatat ctggcgggtg atacgaagat tcttaataac atcaattttt
    13501 cgctgcgtgc tggcgaattt aagttaatta ccggtccttc tggttgtggc aaaagtacgc
    13561 tgctaaaaat agttgcttca ttgatcagcc caaccagcgg aacgttactg tttgaaggtg
    13621 aggatgtcag cacactaaaa ccagaaatct accgccagca agtctcttac tgcgcccaga
    13681 cgccgacgct gtttggcgat acggtatacg ataatctgat ctttccctgg cagatccgtg
    13741 accagcagcc tgacccggcc atttttctcg attttctcga acgcttcgct ttgccggaca
    13801 gcattttgac gaagaatatc gccgagctat ctggtggtga aaaacaacgc atctcattga
    13861 ttcgtaacct gcaatttatg ccgaaggttt tattgctgga tgaaataacc agtgcgctgg
    13921 atgaaagtaa taaacataac gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata
    13981 ttgccgtgct gtgggtgaca cacgataaag acgaaattaa tcatgcggat aaagtgatta
    14041 cactgcaacc gcatgccgga gaaatgcagg aagcacgcta tgaactcgca taatattact
    14101 aacgaatcat tagcactggc attaatgctg gtggtggtgg caatcttaat tagccataaa
    14161 gaaaaactgg cgctggagaa agatattctc tggagcgtcg ggcgagcgat aattcagctg
    14221 attattgtcg gctatgtgct gaagtatatt ttcagcgtgg atgatgccag cctgacatta
    14281 ttgatggtgt tatttatctg ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat
    14341 attgctaaag cttttatctc atcgtttatt gccattacgg tcggggcggg aattaccctg
    14401 gcggtgctga ttctctcagg ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc
    14461 gggatgattg ccggtaacgc catggtagcg gtggggttgt gttacaacaa tttagggcaa
    14521 cgggtcatta gcgaacagca acagattcag gagaaactga gtcttggcgc gacgccgaag
    14581 caggcttcag cgatattgat tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat
    14641 tcagcaaaaa cggttggctt agtgagttta ccaggaatga tgtccgggct gatatttgcc
    14701 gggattgatc cggtgaaggc gattaaatat cagattatgg tgacctttat gctgctctca
    14761 accgccagct tgtcgaccat tattgcctgc tatttaacct atcgtaagtt ttataattcg
    14821 cgccaccagt tggtggtgac tcaattgaag aagaaatgat gcgatgccgg atgcgcagca
    14881 tcgcatccgg cgttgtggtt catgtgccga acaacggaat atcaatacaa caatgcatac
    14941 agctggcggc gatacttcga cgccagtgca tcacccgtac ccagcgcagc gaggatctcc
    15001 tggaacgttt tacgcgtctg accgtctgcg gcggtgagat ctttacgcag atgcccgaac
    15061 agcaactcca gcgcctcttc attgcgccca acctgatgca gttgcagcgc cagttgcgtc
    15121 gccagtgcgg catcttctgg attctccgcc acctgctgtt gcaactgttg aatttccggc
    15181 ttatcagccg cctgcttcag cagttcgatt tgcgctacca gcccctggta gcgggtgtcc
    15241 tgatcctgca acggaatggt tttcagcacc gcttccgcat cttcagaacg gttcagcgca
    15301 atcagcgttt ctgccagcag caggccgatc tccccgttct gattcgacaa ctgccaggcg
    15361 tcttgcagca atggcagggc atcggtgtaa ttgccttcct gcatcagttg catcgcctgc
    15421 tgcgctttca gctcttcttc gcgcggcagc actttatcca gcagggcgcg gatcgcctct
    15481 tccggttgcg gcccctggaa gccatctacc ggttgcccgt tctggaacag atacacggtc
    15541 ggaatcgcgc gcagaccaaa ctgggcggca atcatctgct ccgcgtcgca gtccagcttc
    15601 gccagaataa attgcccgtt gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc
    15661 aaacagtgct ggctacgttc agaccaaaaa tagaacagca ccggagtggt catcgactgt
    15721 tcaagaacct gttgcaggtt agattcgtta atgttgacaa tattttctac ggacatggag
    15781 tcgctctctg ttgtcgatat tttctttgac atgggggctt aagcgcgcgc ttcaactcac
    15841 ccctgcaata ttttgtccat cacgcgccca ggcagcaggc gctcgagcac cattactgcc
    15901 caggtcacca gcgttaccgg atagcgcatc ttcggcttct cgctaataaa agcatggcgc
    15961 actttgtcga ccaccgcttc cggtcccaac gtaaagcggg cggcgatgcc gggattttcg
    16021 actggtttat cactttgcgt ctggttgacg ttgtcggtga agcgagtacg aatgggaccg
    16081 ggttcgatca ggctgacttt aattccgctg tggcgtagct ccatgcgcag cgcgtctgac
    16141 cacgcctcca gcgcatattt actggccgcg taagcgccac gacccggcgt ggagattaat
    16201 cccatcaccg atgatgtcat cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac
    16261 aggcgcatgg tgagctggtg tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc
    16321 gcacggctga tggtggaaag ggggccatac atgccgaatc cggcattgtt aaagatccca
    16381 tacagacaat tatcggtcag ggcgatcacc tcgtctgctg cgcgattaac actttctggt
    16441 gaatccagat cgatcaacac gccggtaaat cccatgttgt tcatacgctc aacatcattc
    16501 ggttttcggc aacctgccag cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc
    16561 aggccaattc cactggaaca tccggtaatt aagaccgatt tttgcataac tttacctgtc
    16621 aggatctccg ttgctttatg agtcatgatt tactaaaggc tgcaactgct tcgccatcca
    16681 gtcggcaata aacggctggg cgtcgcggtt gggatgaata ccgtcatcct gcatccattg
    16741 tggcttgagg tagacctctt ccataaaaaa gggcagcagc ggaacatcaa actctttggc
    16801 gagtttgggg taaatggcgc taaaggcttc attataacgg cgaccatagt ttgcaggcag
    16861 acgtatttgc attaacaatg gttcagcgtt ggcggctttg acatcctgca aaatctggcg
    16921 cagcgtttgc tcggtttgct gtggctgaaa accacgcaaa ccatcattgc gcccagttca
    16981 accagcaccc aacgcggctg atgctgtttg agcagagccg gaaggcgcgc cagcccttgt
    17041 tgcgaggtgt cgccgctgat gctggcatta actaccgacg ttttactctg ccacttatca
    17101 ttcaacaagg caggccaggc cgcgctggca gacattcgat acccggcgct caggctatca
    17161 cccagaatca ataacgtgtc cgctgcggcg gcacggaagg ttaacaggac caggaacagg
    17221 aagggcaaat gccagcggaa aacattgttg aagttcatca tcttaagaag tccgtcggtc
    17281 agggggagca tgaactctcc atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga
    17341 ccatcgcact ggtgggcgag tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg
    17401 ggcttgatga cggcagcagt ggcgaagtga gtctggtggg acaaccgcta cataatatgg
    17461 acgaagaagc gcgggcaaag ttgcgcgcga agcacgtcgg ctttgttttt cagtcattta
    17521 tgttaattcc tacccttaac gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg
    17581 agagtagcgc ggaaagtcgt aacggggcga aagcgttgct cgaacagtta gggctgggta
    17641 aacgtctgga tcatcttccg gcacagcttt ccggcggtga acagcaacga gtggcgctgg
    17701 cacgagcctt taatggtcga cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg
    17761 accgccagac gggcgataaa attgccgacc tgctgttttc cctcaaccgt gaacatggca
    17821 ccacgttgat tatggtgacc cacgacctgc aactggcggc acgctgcgac cgctgcttac
    17881 ggctggtgaa cgggcagttg caggaggaag catgattgca cgttggttct ggcgcgaatg
    17941 gagttcgccg tcgctattaa ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct
    18001 ggcgctgggc aatatcagcg atcgcatgga gaagggctta agccagcaaa gccgtgagtt
    18061 tatggcgggc gatcgggcgt tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga
    18121 agcgcaaaag cgcggcctga aagtcggcaa gcagctgact ttcgccacaa tgacctttgc
    18181 aggcgacaca ccgcagctgg cgaacgtcaa agcggtggat gatatctacc cgatgtatgg
    18241 cgatctgcaa actaatcccc ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc
    18301 acgcctgatg gcactgctta acctgaaaac gggcgacacc attgacgtgg gcgatgccac
    18361 cttgcggatt gccggagaag tgattcagga accggattcc ggttttaacc ccttccagat
    18421 ggctccgcgt ctgatgatga atctggcgga tgtcgataaa accggagccg tgcagccggg
    18481 gagtcgggtc acctggcgtt ataaattcgg cggcaacgag aaccagctcg acggctatga
    18541 gaaatggttg ttacctcagc ttaaacccga acaacgctgg tacggtctgg aacaggacga
    18601 aggcgcgctg gggcgatcga tggaacgctc gcaacagttc ctgctgcttt cggcgcttct
    18661 gaccttgctg ctggcagtgg cagcggtggc ggtagcgatg aatcattact gtcgcagtcg
    18721 ctacgatctg gtggcgatcc tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct
    18781 aatcgtcggt cagtggttga tggtgctgac gctttcagcc gttaccggtg gggccatagg
    18841 cctgttgttc gaaaacgtgt tgatggtgct gctcaagccc gttctgcctg ctgcactacc
    18901 gccagccagc ctctggccgt ggctgtgggc gcttggcacc atgacggtca tctcgctgct
    18961 ggtggggcta cgaccatacc gcttgttgct ggcaacgcag cctttacgcg tattacgtaa
    19021 tgatgtggtc gcgaacgtct ggccgctgaa gttttatctg ccgattgtca gtgtggtggt
    19081 tgtgctgctg ctcgccggat taatgggtgg cagcatgctg ctttgggcgg tgctggcggg
    19141 cgcggtagta ctggctttgc tgtgcggtgt gctgggctgg atgctgctga atgtacttcg
    19201 ccgcatgacg ctgaaatcgc tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc
    19261 gtggtcaacg ttaagccagc tttcggcatt ttcgctctcc tttatgctgc tggcactgct
    19321 gctggtgctg cgtggcgatc tgctcgaccg ctggcagcag cagctaccgc cagaaagccc
    19381 gaactacttt ttaattaaca tcgccacaga acaggtagca ccgctaaaag cgttcctcgc
    19441 ggaacatcat atagtcccgg aatcatttta tccggtagtg cgggcgcggc tgacggcgat
    19501 taatgataag ccgacagaag gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac
    19561 ctggcaaaat acgcggcccg atcataatcc gattgtcgcc ggtaactggc cgccaaaagc
    19621 cgatgaagtg tcgatggaag aggggctggc gaaacgctta aacgttgccc tcggtgatac
    19681 cgtgactttt atgggcgata cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt
    19741 ggactgggaa agtctgcggc ctaatttcta ttttattttc cctgaagggg cattagacgg
    19801 gcaaccgcag agctggctta ccagtttccg ctgggagaat ggcaacggca tgttgacgca
    19861 actcaaccgc cagttcccga ccattagcct gttagacatt ggcgcgattt taaaacaggt
    19921 cggtcaggtg ctggagcagg taagtcgggc gctggaggtg atggtggtgc tggtcaccgc
    19981 ctgcggtatg ttgctgttgc tggcacaggt gcaggtggga atgcgccagc gtcatcagga
    20041 gctggtggta tggcgcacac tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg
    20101 tgatgttcgc catgcttggg tttgtttccg gcctggtggc cgcaattggt gcggaaacgg
    20161 cactggcagt gttgcaggcg aaagtgtttg atctcccgtg ggagccagac tggcgattgt
    20221 ggattgttct gccgtgcagc ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg
    20281 cgcgactggt taagggtaag gcgctgttca ggcagtttgt ggggtgatga caggtgttag
    20341 attgcctgat gcgctacgct tatcaggcct acgaagagcg tgcaatatat tgattttgca
    20401 agattgtgta ggccggataa ggcgtttacg ccgcatctgg catgaacagt gtgcattttg
    20461 gcaataaata tgaaaccaac tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct
    20521 tcggccttag ctacgtttcc tcagttattc aataaactga cggatcagtt ccagcagcgc
    20581 gattagcgcc ttaagaacga tgagaacttt atcgagaaaa gttttcatcc gttgctccct
    20641 taagggagaa agacaatcac agcttaccct tacgttgcct ataactatga ttttcttcat
    20701 gtgctagatg aggactgtct ctctccaaat tagagcacca gaatcagcac cacctgtatc
    20761 ctgaccagga cttaaaagta cttttccgca ctgcggtaat cccttgaggc agtatcacta
    20821 ctagcgtcgg gattgtatcg ccaacatagc tgtttgaata tgtaggcatc ctgacttata
    20881 attcacttgc tagatgagga ctgtttaatt tctgcccgga accaccaagt cttgggcgga
    20941 aaaaataaaa aaaccgactg tcatgtcggt tttttatatc tgtattctgg tgcaaccatt
    21001 ttgttctcac gacaatcgcc gtttcagaat accgcccatc actgcgcctt aacccatacc
    21061 gccacttccg cccactcacc gcgaaagaca actttttccg cttttttctc aagctgatag
    21121 cgatacattg ggtcgtaata ttctttaagt aacggcacca gccaggccag atgaccgtcg
    21181 gtgctgccgg tggtgagttg cgttgtcagc gccgtatcca gctgggcagc gagttcgtta
    21241 tagcgctgta accccagccg acgcttaatc gccgaaagtc cgtgatgcag gtattcgcaa
    21301 tattcctgcc agccctgttc gtcgccataa gcgtgggtaa aatcatgatg catacgcaag
    21361 aaatactctt cattcaggcg ttcaagacgg atctcaaacg gatcttctac caccgcaatc
    21421 gccgcctgag tcattcgctc gcgcagacat tccggcaggt gattcgaacc gatcatccgg
    21481 ctttcatctt ccagcaccca caggcgcaaa tcctgacggg cgtcggtttt tagcatttcg
    21541 gcagccagca ggttttcaaa actcgcctgg ctaagttgtg gttgtaacgt gcgaccaaac
    21601 gccgaaccgc gatgacgcgc cagcccttcc agatcaacac cgtttggctg ttgttgcact
    21661 aatagcgttt taccgctgcc ggtacaaccg ccaatcagca ctatcggttt ttgtgccagt
    21721 tcaatggtcg cctgaatcgc ggtctggcgc agtgccttat aaccgccttc caccagcgga
    21781 taatcaatcc ccgcttcatg cagccagcgt tgcacaatat gtgaacgttg accgccacgg
    21841 gcgcagcaga gaataccttg cggattttgc aggcacgctg cccgccaggc gtacatacgc
    21901 tgctgacgaa tttcacccgc caccagttta tgccccagcg ccagcgctgc gtctgagcct
    21961 tgctgtttat agcaggtgcc aacggaggcg cgttcatcgt tatccattaa cggcagattg
    22021 atagcggcgg gcattgcgcc ttgctcaaac tcgatagggg cgcgaacatc aataatgggc
    22081 gtatcagcaa tcagcagggc acgatagtcc tgttccgtgt gtctctcttg catagttaaa
    22141 agtgaacctc aaatcagctt gcgcgctatt ttacgcgcca acgcgcaagg aaacttgatt
    22201 tttaactgcg tggggtgccg aaaatttcta aaaatccgct gatttccggc ctgtgttggg
    22261 taaacaaggt cacaatatct tctaccgctt tgcagctgcc aaatttgcgc catgccagac
    22321 tcaatggcga aggagggcgc atcgttggga ttacccggct gaccagttgt tgattatcga
    22381 tcattgactg gcaaagtgat tttggcaaaa aaccaatgcc aacacccgcc agatgggcgg
    22441 cgattttcgt ttccatatcg ggaacaataa tctctttttg ccccggcaat cgccaggcga
    22501 cgcgtttggt taaggtgccg gcgctgtctt caatattgac cgccggaaag cgacgcaact
    22561 gcgcttctgt tagcggctct tcaacgttcg ccagcggatg atccgccgcc atgacaaatc
    22621 gccattgcac cgatcctaag ggatcaagac taaaggtatt tgccagcgcc tcagttcccg
    22681 tgaccccgat agccagcgaa aaaccttcgt acaatagcga gtcccagacg cccatataga
    22741 tttgtcggga gatgtgaaac tgggtaaagg ggtaacgctc attcagccac gccagcaact
    22801 gggcgacggc ctgggggtta tagagcaggt tgttgatgac aatattcacc tggcgttcca
    22861 cgccatcatt cacctgttgc agctcgctgg gcatactttc cagccagctc agccagtctc
    22921 tggcctggga aagtagatgc tcgccagccg ctgtcaacgt tacgctacga gtcgtacgga
    22981 aaaacagcgc tactccggta ttctcttcca gaagtttaat gcgataactg atcgtcgccg
    23041 tggttttaca taatcgttct gccgcttttg aaaaacttcc tgtttcagca accgcaatga
    23101 aagtccgcaa ggtttctgga tcgaacatct tcaggtatcc ccttttaaat ccgcaagttg
    23161 cgtgattttc ttatcctctg atttatcagt atttttacat gataatcctg ttcaatttgt
    23221 ggactaaatc tagttttgga aaaacattcc aacttttgta ttgatgttgt tctcttaagg
    23281 ttttagattg cctgttattg aaaccaagct gaccggtcgg cggtggttga acggaattat
    23341 gttacaagga caaaaagatg aaacttcagg tattaccgtt aagtcaggaa gcctttagtg
    23401 cttatggcga cgtaatcgaa acgcagcaac gggatttttt ccatattaac aatggcctgg
    23461 tggagcgtta ccacgatttg gcgctggttg agattcttga gcaagaccgt acgcttatca
    23521 gcattaaccg cgcgcaaccg gcgaatctgc cgctgaccat tcacgaactc gaacgtcatc
    23581 cgctgggcac tcaggccttt atcccaatga aaggtgaagt gtttgtggtg gtcgtggcgt
    23641 taggcgacga caaaccagac ctgtcaacgc tgcgggcgtt tatcaccaac ggtgaacagg
    23701 gggtgaatta ccatcgtaac gtctggcatc acccactttt cgcctggcag cgcgtcaccg
    23761 attttctgac catcgatcgc ggcggcagtg acaactgtga tgttgaaagt attcctgaac
    23821 aggaactctg ttttgcgtga cgcctgcaac cgacttgcat aagataaact aattgttcat
    23881 tgtttatgct cacttgtagg tcggagttaa cgtaggtatg acggaagtta gacggcgcgg
    23941 caggccagga caggcggagc ctgtggcaca gaagggcgca caggcgttag agcggggaat
    24001 tgcgattctg caatatctgg aaaaaagtgg gggaagttcg tcggttagcg atatttctct
    24061 caatctggat ttgccgctct ccacgacctt tcgcttgctg aaggttttac aagcagcgga
    24121 ttttgtctat caggacagtc aattaggctg gtggcatata ggattaggtg tctttaacgt
    24181 cggtgcggcg tacatccata accgcgatgt cctctccgtc gccgggccgt ttatgcgccg
    24241 cctgatgtta ctttccggcg aaacggtcaa tgtcgcgatc cgtaacggca atgaagcggt
    24301 attaattggt cagttagagt gtaaatcgat ggtcaggatg tgtgcgccac tgggcagtcg
    24361 tctgccgctg catgcttccg gtgcgggcaa agcgctgctt tatccgctgg cggaagagga
    24421 gttgatgagc atcattctgc aaaccggttt gcagcagttt acgccaacta cgcttgtgga
    24481 tatgcccacc ttgctgaagg acctggaaca agcgcgtgaa ctgggctata ccgtagataa
    24541 agaagagcat gttgtaggtc tgaattgcat agcttcagca atttacgatg atgtcggtag
    24601 agttgttgcc gctatctcca tctccgggcc ttcatcaaga ctgacagaag atcgttttgt
    24661 cagtcagggt gagctggtca gagacaccgc ccgcgatatc agcacggcgt tgggactgaa
    24721 agcacatcca tagtgtctgt cgcatcccgc tctgcggagc gggttttttt gacaaaattt
    24781 gaaagttgga aaatttttcc aattaataga ggtaggaata aaatggcaaa aatgagagcc
    24841 gttgacgcgg caatgtatgt gctggagaaa gaaggtatca ccaccgcctt cggcgttccg
    24901 ggagctgcaa ttaatccgtt ctactcagcg atgcgtaagc acggcggtat tcgtcacatt
    24961 ctggcgcgtc atgtggaagg tgtttcgcac atggcggaag gttatacccg cgcaacggca
    25021 gggaatatcg gcgtatgtct ggggacttcc ggtcctgcgg gcacggacat gatcaccgcg
    25081 ctctattccg cttctgctga ttccattcct attctgtgca ttaccggcca ggcaccgcgc
    25141 gcccgtctgc ataaagaaga ttttcaggcc gtagatattg aagcaattgc taaaccggtc
    25201 agcaaaatgg cggttacagt tcgtgaagcg gcgctggtgc ctcgcgtgct gcaacaggca
    25261 tttcacctga tgcgttctgg tcgtccgggt ccggtactgg tggatttacc gttcgacgtt
    25321 caggttgcgg aaatcgagtt tgatcctgac atgtacgaac cgctgccggt ctacaaacct
    25381 gctgccagcc gtatgcagat cgaaaaagct gtagaaatgt taatccaggc cgaacgtccg
    25441 gtgattgttg ccgggggcgg ggtaatcaat gctgacgcag ctgcactgtt acaacagttt
    25501 gctgaactga ccagcgttcc ggtgatccca acgctgatgg gctggggctg tatcccggac
    25561 gatcatgaac tgatggccgg gatggtcggt ctgcaaaccg cgcatcgtta cggtaacgca
    25621 acgttgctgg cgtccgacat ggtgtttggt atcggtaacc gttttgctaa ccgtcatacc
    25681 ggttcggtag agaaatacac cgaagggcgc aaaatcgttc atattgatat tgagccgacg
    25741 caaattggtc gcgtgctgtg tccggatctc ggcattgtct ctgatgctaa agcggcgctg
    25801 acactgctgg ttgaagtggc gcaggagatg caaaaagcgg gtcgtctgcc gtgtcgtaaa
    25861 gaatgggtcg ccgactgcca gcagcgtaaa cgcactttgc tgcgcaaaac ccacttcgac
    25921 aacgtgccgg tgaaaccgca gcgcgtgtat gaagagatga acaaagcctt tggtcgcgat
    25981 gtttgttatg tcaccactat tggtctgtca caaatcgctg cggcacaaat gctgcatgtc
    26041 tttaaagacc gccactggat caactgtggt caggctggtc cgttaggctg gacgattccg
    26101 gcggcgctgg gggtttgtgc cgctgatccg aaacgcaatg tggtggcgat ttctggcgac
    26161 tttgacttcc agttcctgat tgaagagtta gccgttggcg cgcagttcaa cattccgtac
    26221 atccatgtgc tggtgaataa cgcctatctc ggcctgattc gtcagtcaca acgcgctttt
    26281 gacatggact actgcgtgca actcgctttc gagaatatca actccagtga ag
//
LOCUS       DAJTUN010000049        26300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTUN010000049 DAJTUN010000000
VERSION     DAJTUN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.049"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(1..312)
                     /locus_tag="OO470_001918"
     CDS             complement(1..312)
                     /locus_tag="OO470_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6251176.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            475..571
                     /gene="ffs"
                     /locus_tag="OO470_001919"
     ncRNA           475..571
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO470_001919"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            691..1044
                     /locus_tag="OO470_001920"
     CDS             691..1044
                     /locus_tag="OO470_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6251177.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1086..2636)
                     /gene="pdeB"
                     /locus_tag="OO470_001921"
     CDS             complement(1086..2636)
                     /gene="pdeB"
                     /locus_tag="OO470_001921"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6251178.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2800..3270)
                     /locus_tag="OO470_001922"
     CDS             complement(2800..3270)
                     /locus_tag="OO470_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6251179.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3386..3937)
                     /gene="maa"
                     /locus_tag="OO470_001923"
     CDS             complement(3386..3937)
                     /gene="maa"
                     /locus_tag="OO470_001923"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6251180.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4109..4327)
                     /gene="hha"
                     /locus_tag="OO470_001924"
     CDS             complement(4109..4327)
                     /gene="hha"
                     /locus_tag="OO470_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6251181.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4353..4727)
                     /gene="tomB"
                     /locus_tag="OO470_001925"
     CDS             complement(4353..4727)
                     /gene="tomB"
                     /locus_tag="OO470_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6251182.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5273..8422)
                     /locus_tag="OO470_001926"
     CDS             complement(5273..8422)
                     /locus_tag="OO470_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6251183.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8445..9638)
                     /gene="acrA"
                     /locus_tag="OO470_001927"
     CDS             complement(8445..9638)
                     /gene="acrA"
                     /locus_tag="OO470_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6251184.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9780..10427
                     /gene="acrR"
                     /locus_tag="OO470_001928"
     CDS             9780..10427
                     /gene="acrR"
                     /locus_tag="OO470_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6251185.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10555..13917
                     /gene="mscK"
                     /locus_tag="OO470_001929"
     CDS             10555..13917
                     /gene="mscK"
                     /locus_tag="OO470_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6251186.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14129..14290)
                     /gene="rsmS"
                     /locus_tag="OO470_001930"
     CDS             complement(14129..14290)
                     /gene="rsmS"
                     /locus_tag="OO470_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6251187.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14304..14831)
                     /gene="priC"
                     /locus_tag="OO470_001931"
     CDS             complement(14304..14831)
                     /gene="priC"
                     /locus_tag="OO470_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6251188.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14901..15278
                     /locus_tag="OO470_001932"
     CDS             14901..15278
                     /locus_tag="OO470_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6251189.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15431..15982
                     /gene="apt"
                     /locus_tag="OO470_001933"
     CDS             15431..15982
                     /gene="apt"
                     /locus_tag="OO470_001933"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6251190.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16111..18036
                     /gene="dnaX"
                     /locus_tag="OO470_001934"
     CDS             16111..18036
                     /gene="dnaX"
                     /locus_tag="OO470_001934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6251191.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17369..17433
                     /gene="dnaX"
                     /locus_tag="OO470_001934"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18089..18418
                     /locus_tag="OO470_001935"
     CDS             18089..18418
                     /locus_tag="OO470_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6251192.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18418..19023
                     /gene="recR"
                     /locus_tag="OO470_001936"
     CDS             18418..19023
                     /gene="recR"
                     /locus_tag="OO470_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6251193.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19133..21007
                     /gene="htpG"
                     /locus_tag="OO470_001937"
     CDS             19133..21007
                     /gene="htpG"
                     /locus_tag="OO470_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6251194.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21188..21832
                     /gene="adk"
                     /locus_tag="OO470_001938"
     CDS             21188..21832
                     /gene="adk"
                     /locus_tag="OO470_001938"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6251195.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21964..22926
                     /gene="hemH"
                     /locus_tag="OO470_001939"
     CDS             21964..22926
                     /gene="hemH"
                     /locus_tag="OO470_001939"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6251196.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22923..23882)
                     /gene="aes"
                     /locus_tag="OO470_001940"
     CDS             complement(22923..23882)
                     /gene="aes"
                     /locus_tag="OO470_001940"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6251197.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24034..25338
                     /gene="gsk"
                     /locus_tag="OO470_001941"
     CDS             24034..25338
                     /gene="gsk"
                     /locus_tag="OO470_001941"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6251198.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25471..>26300)
                     /locus_tag="OO470_001942"
     CDS             complement(25471..>26300)
                     /locus_tag="OO470_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HCR6251199.1"
                     /translation="LFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLF
                     GHSQRTALTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFA
                     LLEKYLAKTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPL
                     VVIETSRTRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVAS
                     ARAKNPDIEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         6510 a         6765 c         6776 g         6249 t
ORIGIN      
        1 ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata ccatcacacc
       61 ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg aaattgtgcc
      121 atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac gctttaacac
      181 accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat caccgtaagt
      241 ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg gaaatgaatc
      301 ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa aaacgcccga
      361 gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca attagccggg
      421 gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg gctctgttgg
      481 ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag ccaaggcaga
      541 tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct cccaccgttt
      601 cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa gaattgtctg
      661 gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt tgtggttgcc
      721 gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc gccattgttt
      781 aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc ggatggcaaa
      841 gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt ctttagctgg
      901 attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat gtcggaccca
      961 cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat ctatggcgga
     1021 ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc cgtcagccca
     1081 aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa tgccttgctg
     1141 tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc ttgtttactg
     1201 gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc gatgatatgc
     1261 ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt cagaatgtcg
     1321 acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc atcaagataa
     1381 atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt cggatcggca
     1441 aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata gtgattgatc
     1501 atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc aagattgatc
     1561 gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa gacgcttctt
     1621 ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg aataaaacta
     1681 tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc agcaccgaca
     1741 attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc acgattttcg
     1801 atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg cagcacaaac
     1861 atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac ttgccgatgc
     1921 cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt catctccagg
     1981 aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg tgttttttgt
     2041 agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac gttatgggca
     2101 ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc aataaaggaa
     2161 gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta acggataatg
     2221 cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat tttacctagc
     2281 tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg aacgttgtta
     2341 tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg catttccatg
     2401 agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag cgcatctttc
     2461 ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt atccagctct
     2521 tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc gacaggcagc
     2581 aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt tctcaccagc
     2641 atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag gctggcagga
     2701 gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca ttgtcatttg
     2761 accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg attcggcgcg
     2821 agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca ttttttgcag
     2881 ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa ccagcgcggc
     2941 aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat ccagcacgtc
     3001 gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat taagcaggat
     3061 aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca acatcaccgc
     3121 cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt tacggataaa
     3181 actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag actcaatcgt
     3241 ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct gcaactttcc
     3301 agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc taccacaatt
     3361 ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct ggattaccgc
     3421 ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct accacgacgt
     3481 tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag acgttattac
     3541 cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg tcgatgggat
     3601 gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg atgcgaatag
     3661 ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg agaaaaatgt
     3721 tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc tgaccgaata
     3781 aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga ttgtatcggt
     3841 gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc gagcgataca
     3901 actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg ttgctgaaaa
     3961 tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac atctgtgtga
     4021 taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta ataaacagcc
     4081 ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact gaggaaggga
     4141 tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg gctgagtaaa
     4201 ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca cgctccagcg
     4261 tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt aaaggttttt
     4321 cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc aggattctct
     4381 ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca tttctgaaga
     4441 gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag atattcgtcg
     4501 atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag tgcgaaggtc
     4561 gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg gtcgttaacc
     4621 caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt ttcacagaga
     4681 aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac gcgtcccctt
     4741 cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga cctggacttg
     4801 ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc gcattattac
     4861 gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact agcactaacc
     4921 cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt atcgccagat
     4981 gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc gaggaatgaa
     5041 taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt attaaatcta
     5101 ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt ttatacgtgg
     5161 ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct taatgttcgt
     5221 aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg tatcaatgat
     5281 gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg cggcgaacca
     5341 ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc atcccgccca
     5401 ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg ataaccagcg
     5461 gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa cgcatccgca
     5521 ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag tctttggcga
     5581 attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc agtcctactt
     5641 ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca ccgataaccc
     5701 ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac agcgccgcca
     5761 gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg ttgccggaga
     5821 gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc agtttgctcg
     5881 ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc gcctggccta
     5941 agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg tactcccaac
     6001 gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga acataccagt
     6061 cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa actttcttca
     6121 cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg cccagagtgg
     6181 tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg atatcaatct
     6241 taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata tcagggtgct
     6301 tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga ccaaggccag
     6361 cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg attgcgggca
     6421 ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt gcacgcatgg
     6481 taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc aaggaaacga
     6541 acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg gcgaacaccg
     6601 actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg agcactttct
     6661 gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat acgccctggt
     6721 cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg ccgaccacga
     6781 tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct acgctgtcgg
     6841 tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag ccttttttac
     6901 cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga gccggagtca
     6961 ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata gagaactgac
     7021 gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc gccgacagta
     7081 ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg gtagcttctt
     7141 ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca acgatagcgt
     7201 catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg tttatcgaga
     7261 agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg gcaatggtcg
     7321 gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac acgaggatga
     7381 tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac ggcgtggtgt
     7441 cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc gccagttcag
     7501 cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg atccccagac
     7561 cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca cccagctcaa
     7621 tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc agcaggattt
     7681 tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg ttaagctgtt
     7741 ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg ttctgcgctt
     7801 tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca ttcgggttca
     7861 tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg cccgacgtac
     7921 ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc tgcgtcatgg
     7981 tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat ttctcaacgc
     8041 tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc agtttgttct
     8101 gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg gtgatctgca
     8161 cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata ccgttcatat
     8221 tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg ccggggtagg
     8281 aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc ggcagtttga
     8341 ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca aaaatcgggc
     8401 gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga cttggactgt
     8461 tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc ttgtgctttt
     8521 acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc gcctgctttc
     8581 agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat cggacgggtt
     8641 tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg cgtacgggtt
     8701 acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag acgtgcgcgc
     8761 acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg tagggtgata
     8821 gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc ctgcgggaac
     8881 ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg tttcagcgtg
     8941 ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt cacatcaacg
     9001 tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt ctgtaccaat
     9061 gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt gactttggtg
     9121 taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac cgcagcattc
     9181 gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta ctgagtaccg
     9241 agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc ctgggctttc
     9301 gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc aatctgatag
     9361 agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag gataatcccg
     9421 ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg aagctcggtt
     9481 gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat ctgctggcca
     9541 ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga gagcatcaga
     9601 acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg agtgtccgat
     9661 ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata ttatcgtcgt
     9721 gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt cacgaacata
     9781 tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc gatgtggctc
     9841 tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt gcaaaagcag
     9901 ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat ttgttcagtg
     9961 agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat caggcaaaat
    10021 tccctggcga tccactctca gtattaagag agatattaat tcatgttctt gaatccacgg
    10081 tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc gaatttgtcg
    10141 gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt tatgaccgta
    10201 tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat ttaatgacgc
    10261 gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac tggctctttg
    10321 ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc ttactggaga
    10381 tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc tgaatctgac
    10441 tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg tcgtgatatt
    10501 cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt catcatgact
    10561 atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat tgcttttgtt
    10621 tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg tgatctgccg
    10681 acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga tctttctgct
    10741 caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga taaaatcgat
    10801 cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc ggaaaaaatg
    10861 cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga agaaacgcgc
    10921 aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca ggcgctggac
    10981 gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt ttcgttacag
    11041 acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct gcaacaaatt
    11101 cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag ccagaaagtg
    11161 ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg taaaagcctg
    11221 gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt gacggcgaac
    11281 agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag caagcgcctg
    11341 actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc gcgtattcag
    11401 gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca gcgtctgatt
    11461 accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa aaactggctg
    11521 gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct gaagggcagc
    11581 ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc ggatgaactg
    11641 gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt taaccagcag
    11701 cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg tcacaccaac
    11761 gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg tcgcgaattg
    11821 ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa cctgcaaatc
    11881 aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac tcagcaaatc
    11941 ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt cccgcaaagc
    12001 ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc ctggcctgcc
    12061 gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct gatccactgg
    12121 cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg ttccctgcgt
    12181 aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg cgcgctgccg
    12241 gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct caacatcagc
    12301 gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt tggcctgtgc
    12361 tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc ggagcagcag
    12421 accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc tatccatttc
    12481 tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct ggggcaggcg
    12541 atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg ccgcgaaagc
    12601 tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct gtcgattatc
    12661 ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct gcgcctctcc
    12721 gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta ccagacggta
    12781 ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct ggcgcgtcgg
    12841 cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc caccattgca
    12901 ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt tgcgctgttc
    12961 ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta tctcgacagc
    13021 atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa aaacgtcacc
    13081 atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt gattcgcaac
    13141 ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca gggcgcgtcg
    13201 tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat gacggtgttc
    13261 ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt atccgtaggt
    13321 cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat cattctgttc
    13381 gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg gacggtaagt
    13441 aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt gatcatcccg
    13501 aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac tactacgcgt
    13561 ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg taaagtgtta
    13621 ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga agtcttcttt
    13681 acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg tgaactgcgt
    13741 gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg ccgtgaaaac
    13801 gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa gggcgatgag
    13861 gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt tgggtaataa
    13921 ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt tgttcatgcc
    13981 ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat atattgcagg
    14041 agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc cgcaatctta
    14101 ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg tgcgttaatt
    14161 tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg gcagggatct
    14221 gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca ggggcatttt
    14281 caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt ttcgattttt
    14341 tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc acgatgcagc
    14401 gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc ggcaaccatc
    14461 tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca gcgggaaatt
    14521 ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg cgcgatggct
    14581 tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg ttgctgctca
    14641 actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga ctgtagtgtt
    14701 gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc gaactgtgac
    14761 accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc cagcagcagg
    14821 gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag tagccactat
    14881 gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc tggctggcgg
    14941 tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg tttatcctgc
    15001 tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg ctgtaccgct
    15061 catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg cgcggcgtca
    15121 aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg ttcgtccaga
    15181 tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc tatatgtggc
    15241 gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc gcagttgcaa
    15301 ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc ggtcaaaggt
    15361 taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg taacaagcag
    15421 gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata gcatcaaaag
    15481 cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct tactggaaga
    15541 cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa atgcgggcat
    15601 taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg tagctctggg
    15661 tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg aaaccatcag
    15721 tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg atgccatcaa
    15781 accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca ctatcgaagc
    15841 gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt tcattatcaa
    15901 cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca gctacagcct
    15961 tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct acggacagca
    16021 caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac cccttcgtga
    16081 atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc ccgaaaatgg
    16141 cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc actggcgaac
    16201 ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg tggcgtcgga
    16261 aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg cattaccgcg
    16321 acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt tgtcgatctg
    16381 attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct gctggataac
    16441 gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga agtgcatatg
    16501 ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc ggagcacgtt
    16561 aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt gtcacgctgt
    16621 ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct tgagcacatc
    16681 ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc gcgcgccgct
    16741 gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag cggtgacggc
    16801 caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga tcaggcgctg
    16861 tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact gattaatgaa
    16921 gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg cttgttgcat
    16981 cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc cgccatcgag
    17041 ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct ttactatcag
    17101 acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat gggcgttgag
    17161 atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga gccagaagtg
    17221 ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac cttggtgccg
    17281 ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac cagccaggtg
    17341 ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa aaagagtgaa
    17401 ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag actggcttcg
    17461 gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc gccagccaaa
    17521 aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga agtggtcgcc
    17581 acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact ggcggtgaag
    17641 ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca actttcgcta
    17701 ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga caacgcagta
    17761 tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca gcaaaaactg
    17821 gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt tgaagatgat
    17881 aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga aaaacttgcg
    17941 caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg gtttttcgat
    18001 gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct tacgttcgtc
    18061 atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg gtaacctgat
    18121 gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga tcgcgcagct
    18181 ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg gtgcacacaa
    18241 ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga tgctggaaga
    18301 cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc agaaagaaaa
    18361 aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc cgttctgatg
    18421 caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc gggcgttggc
    18481 ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag cggcgggatg
    18541 cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc cgattgccgc
    18601 actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca ggaaaacggt
    18661 caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac ggggcagttt
    18721 tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat cggtccggat
    18781 gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac tgaagtgatc
    18841 ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc cgagctttgc
    18901 gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg cggcgaactg
    18961 gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa gattcgtttt
    19021 taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct tgtccccatc
    19081 tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct acatgaaagg
    19141 acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga tgatccattc
    19201 tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct ccgatgcggc
    19261 ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg atggcgaact
    19321 gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg ataacggcgt
    19381 ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat ccggtaccaa
    19441 atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga tcggtcagtt
    19501 tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc gtactcgcgc
    19561 ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag gtgaatatac
    19621 cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc tgcgtgaagg
    19681 cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat actccgacca
    19741 tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa ccgttatctc
    19801 ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg aaatcaccga
    19861 tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc cgctgacctg
    19921 gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca tcccgtccca
    19981 ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt acgttcaacg
    20041 cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct tcgtgcgtgg
    20101 tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc aggacagcac
    20161 ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc tagaaaaact
    20221 ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc tggtactgaa
    20281 agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc tgcgttttgc
    20341 ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg tttcccgcat
    20401 gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg cagcgaagag
    20461 cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc tttccgaccg
    20521 catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt tccagtcggt
    20581 gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga gcgcgaaaga
    20641 agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg gcgagcgcgt
    20701 gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt cgaccgacgc
    20761 ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga aagtgccaga
    20821 agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg cggcagatac
    20881 tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg cgctgctggc
    20941 agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc agctgctggt
    21001 ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt catcatctgt
    21061 actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt cttgaggcaa
    21121 tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt ttaaggggat
    21181 tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga ctcaggctca
    21241 gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc tgcgtgctgc
    21301 ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg ctggcaaact
    21361 ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg aagactgccg
    21421 taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg cgatgaaaga
    21481 agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac tgattgttga
    21541 tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg ttaaattcaa
    21601 tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta cccgtaaaga
    21661 cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga ctgcaccgct
    21721 gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga aagttgacgg
    21781 taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct aatcaagccg
    21841 cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct ctcacagcaa
    21901 ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg ataagaggcg
    21961 gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc cgatgccccc
    22021 acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg cgtggttgat
    22081 acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct gcgctcgccg
    22141 cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct gatggtttac
    22201 agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt ggcgctgggc
    22261 atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc agagcatgta
    22321 gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt cggtgcggta
    22381 tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat atcgtttatt
    22441 cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt acgcgcttct
    22501 tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat tccccagcgt
    22561 tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg cgaactggct
    22621 tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg ctttggccgg
    22681 gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga aaaaggcgta
    22741 ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac gctggaagag
    22801 attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata tgaatatatt
    22861 ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt tgccgcgtat
    22921 cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc ggcggttttc
    22981 atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt gaactcacag
    23041 ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag cagcggatcg
    23101 aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt attaaacaga
    23161 cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc atacatctgc
    23221 aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt cacggaatcc
    23281 cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca atcgatctgt
    23341 ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga atcaccggca
    23401 aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa ataacaacaa
    23461 gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag ggtgtaatta
    23521 ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg atcgtgggta
    23581 tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc cgggctatct
    23641 ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac catgtaagct
    23701 ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt gtaatactgt
    23761 cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg aagagtattc
    23821 acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt ttccggcttc
    23881 atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga aaatgcgatc
    23941 ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt gtgttaacat
    24001 tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa acgtaaatcc
    24061 aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca gccagaaaac
    24121 gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat tgaagcgaaa
    24181 gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact ggtgattgag
    24241 gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat tacccatcag
    24301 tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc ggacgaccgt
    24361 tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc ctatcgttac
    24421 ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga tggtccgatt
    24481 ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat cagtccaggc
    24541 cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg agcctcggca
    24601 ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc ggaagcaacc
    24661 atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac gctgggcacc
    24721 aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga ccacgtttct
    24781 atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga tccgttgttg
    24841 gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg gccaatcggc
    24901 ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca tccgctgctg
    24961 ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg ccacaaggat
    25021 tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg gccggaaaaa
    25081 atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca tgacattacc
    25141 gccaacagct accatcgtag caacgtacca aactccagca aacataaatt cacctggtta
    25201 acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta tcaggtactg
    25261 aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag ccaggaagag
    25321 tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt tcacgccgca
    25381 tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat gcctacatat
    25441 ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac ccgccggtgg
    25501 cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca ctacctgatt
    25561 cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg cgataatctc
    25621 aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat aaccgttggg
    25681 gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa tttcttcgtt
    25741 cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa cacgggttcg
    25801 tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc ccagcaggct
    25861 gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg ggatctgttt
    25921 ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct tcgccagata
    25981 tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg cccctgccag
    26041 taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc ccgccaggat
    26101 aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg tacgttggga
    26161 gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca gaataattgc
    26221 cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa acaacatccc
    26281 gactgagaca aaaaacagca
//
LOCUS       DAJTUN010000050        25903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTUN010000050 DAJTUN010000000
VERSION     DAJTUN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.050"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            239..801
                     /gene="srlA"
                     /locus_tag="OO470_001943"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             239..801
                     /gene="srlA"
                     /locus_tag="OO470_001943"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            798..1757
                     /gene="srlE"
                     /locus_tag="OO470_001944"
                     /gene_synonym="gutE"
     CDS             798..1757
                     /gene="srlE"
                     /locus_tag="OO470_001944"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6251200.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            1768..2139
                     /gene="srlB"
                     /locus_tag="OO470_001945"
                     /gene_synonym="gutB"
     CDS             1768..2139
                     /gene="srlB"
                     /locus_tag="OO470_001945"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6251201.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            2143..2922
                     /gene="srlD"
                     /locus_tag="OO470_001946"
     CDS             2143..2922
                     /gene="srlD"
                     /locus_tag="OO470_001946"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6251202.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            3027..3386
                     /gene="gutM"
                     /locus_tag="OO470_001947"
                     /pseudo
     CDS             3027..3386
                     /gene="gutM"
                     /locus_tag="OO470_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            3453..4226
                     /gene="srlR"
                     /locus_tag="OO470_001948"
                     /gene_synonym="gutR"
     CDS             3453..4226
                     /gene="srlR"
                     /locus_tag="OO470_001948"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6251203.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4219..5184
                     /gene="gutQ"
                     /locus_tag="OO470_001949"
     CDS             4219..5184
                     /gene="gutQ"
                     /locus_tag="OO470_001949"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6251204.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5181..6695)
                     /gene="norR"
                     /locus_tag="OO470_001950"
     CDS             complement(5181..6695)
                     /gene="norR"
                     /locus_tag="OO470_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6251205.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6882..8321
                     /gene="norV"
                     /locus_tag="OO470_001951"
     CDS             6882..8321
                     /gene="norV"
                     /locus_tag="OO470_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6251206.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8318..9451
                     /gene="norW"
                     /locus_tag="OO470_001952"
     CDS             8318..9451
                     /gene="norW"
                     /locus_tag="OO470_001952"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6251207.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(9579..11831)
                     /gene="hypF"
                     /locus_tag="OO470_001953"
     CDS             complement(9579..11831)
                     /gene="hypF"
                     /locus_tag="OO470_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6251208.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11984..12511)
                     /gene="hydN"
                     /locus_tag="OO470_001954"
     CDS             complement(11984..12511)
                     /gene="hydN"
                     /locus_tag="OO470_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6251209.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12660..13670)
                     /gene="ascG"
                     /locus_tag="OO470_001955"
     CDS             complement(12660..13670)
                     /gene="ascG"
                     /locus_tag="OO470_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6251210.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(13684..13803)
                     /locus_tag="OO470_001956"
     CDS             complement(13684..13803)
                     /locus_tag="OO470_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6251211.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(13813..15522)
                     /gene="hycE"
                     /locus_tag="OO470_001957"
     CDS             complement(13813..15522)
                     /gene="hycE"
                     /locus_tag="OO470_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6251212.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(15540..16463)
                     /gene="hycD"
                     /locus_tag="OO470_001958"
     CDS             complement(15540..16463)
                     /gene="hycD"
                     /locus_tag="OO470_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6251213.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(16466..18265)
                     /gene="hycC"
                     /locus_tag="OO470_001959"
     CDS             complement(16466..18265)
                     /gene="hycC"
                     /locus_tag="OO470_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6251214.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGA
                     GSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKL
                     SNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMS
                     VSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFII
                     MAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSL
                     VPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18302..18913)
                     /gene="hycB"
                     /locus_tag="OO470_001960"
     CDS             complement(18302..18913)
                     /gene="hycB"
                     /locus_tag="OO470_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6251215.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(19038..19499)
                     /gene="hycA"
                     /locus_tag="OO470_001961"
     CDS             complement(19038..19499)
                     /gene="hycA"
                     /locus_tag="OO470_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6251216.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            19711..20061
                     /gene="hypA"
                     /locus_tag="OO470_001962"
     CDS             19711..20061
                     /gene="hypA"
                     /locus_tag="OO470_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6251217.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            20065..20937
                     /gene="hypB"
                     /locus_tag="OO470_001963"
     CDS             20065..20937
                     /gene="hypB"
                     /locus_tag="OO470_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6251218.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            20928..21200
                     /gene="hypC"
                     /locus_tag="OO470_001964"
     CDS             20928..21200
                     /gene="hypC"
                     /locus_tag="OO470_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6251219.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            21200..22321
                     /gene="hypD"
                     /locus_tag="OO470_001965"
     CDS             21200..22321
                     /gene="hypD"
                     /locus_tag="OO470_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6251220.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            22318..23328
                     /gene="hypE"
                     /locus_tag="OO470_001966"
     CDS             22318..23328
                     /gene="hypE"
                     /locus_tag="OO470_001966"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6251221.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            23402..25480
                     /gene="flhA"
                     /locus_tag="OO470_001967"
     CDS             23402..25480
                     /gene="flhA"
                     /locus_tag="OO470_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6251222.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(25517..25870)
                     /locus_tag="OO470_001968"
     CDS             complement(25517..25870)
                     /locus_tag="OO470_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6251223.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         6130 a         7199 c         6878 g         5696 t
ORIGIN      
        1 gattcaacca ggggcaagta tggtaatgca tcacaccccg cacaaggaag cggtagtcac
       61 tgcccgatac ggactttaca taactcaact cattcccctc gctatacttt tattcaaact
      121 ttcaaattaa aatatttatc tttcatttta cgatcaaaat aacactttta aacctttcaa
      181 cctgattaga ttaggttgcc gtttggtaat aaaacaataa atcctgaagg agagaacaat
      241 gatagaaacc attactcatg gtgcagagtg gtttatcggg ctgttccaaa agggcggaga
      301 ggtgtttacc gggatggtga ccggcattct tccgctgttg attagcctgc aggttatcat
      361 gaacgcactg attaatttta tcggtcagca tcgtattgaa cgttttgctc aacgttgcgc
      421 cggtaaccct gtttcccgtt acctactgtt accgtgcatt ggcacgtttg tcttttgcaa
      481 tccgatgacc ttaagcctgg gtcgctttat gccggaaaag tacaaaccca gctactacgc
      541 ggcggcctct tatagctgcc actcaatgaa tggcctcttc ccccatatca accctgcgaa
      601 ctgtttgttt atcttggcat tgccagcggt ctgacaacgc tgaacctgcc acttggccca
      661 ctggcggtga gttatctgct ggttggtctg gtcaccaatt tcttccgcgg ctgggtgacc
      721 gatctgacca ccgccatttt tgagaaaaag atgggcattc aacttgaaca aaaagttcac
      781 ctggcaggag caacatcatg acgcgtattc ggatcgaaaa aggaacgggt ggctggggcg
      841 gcccgcttga gctggaagcc acgccgggca aaaaaatcgt ctatatcacc gccggtaccc
      901 ggcctgcgat tgttgacaaa ctggcacaac ttactggctg gcaggctatt gacggattta
      961 aagaaggtga acccgcggag gcggaaattg gtgtcgcggt aatcgactgt ggcggcacat
     1021 tacgctgcgg catctatccg aaacgacgta ttcccaccat taatatccac tcgacgggca
     1081 agtccggtcc gctggcgcag tacattgtgg aagatattta tgtctctggc gtaaaagaag
     1141 aaaacatcac tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc cgtgactatg
     1201 acaccagtaa gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg ggaatgggca
     1261 tggggtccgc cgttgccgtg ttgtttcagt ctggtcgtga caccatcgac actgtattaa
     1321 aaaccattct gccgtttatg gcattcgtct cggcgctcat tggcatcatt atggcttctg
     1381 gccttggtga ctggattgcc cacggtcttg ctccgctagc gagccatcca ctgggtctgg
     1441 tcatgctagc gctcatctgc tccttcccac tgctttcacc tttcctcggc ccaggcgcag
     1501 ttatcgcaca ggttatcggc gtattgattg gcgtgcagat tggtctcggc aatattccgc
     1561 cgcatctggc tttaccggca ctgtttgcca tcaacgcgca ggcggcttgc gacttcatcc
     1621 cggtcggttt gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt gtcccttctg
     1681 tactggtgag ccgcttttta accggcgcgc cgactgtact gatcgcctgg tttgtctcca
     1741 gttttatcta tcaatagagg ctgaaacatg accgttattt atcagaccac catcacccgt
     1801 atcggcgcga gtgccactga cgccctcagc gaccagatgc tcatcacctt tcgtgaaggc
     1861 gcgccagcgg acctcgaaga gtattgcttc attcattgcc acggcgagtt gaaaggtgca
     1921 ctccatcccg gtttgcaatt ttcactcggg cagcatcgct atccggtgac cgctgttggc
     1981 agcgtggcgg aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt cgatggttta
     2041 aacgaagcgg aatttccggg cactgtccat gtggcaggcc ctgtacccga cgatatcgcg
     2101 ccgggatcgg ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca ggttgccgtt
     2161 gtcatcggtg gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc tgccgagggg
     2221 tatcgcgtcg cggttgtcga tattcagagc gacaaagccg caaatgtggc acaagaaatt
     2281 aacgccgaat atggtgaagg tatggcgtac ggttttggtg ctgacgccac tagcgagcaa
     2341 agcgttctgg cgctctctcg tggggtagat gaaatctttg gtcgcgtgga tttgctggtc
     2401 tatagcgccg gaatagcaaa agcagccttt atcagcgact tccagctcgg cgattttgac
     2461 cgttcgctac aagtgaatct ggtgggttat ttcctgtgtg cccgtgaatt ttcgcgtttg
     2521 atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc cggcaaagtg
     2581 ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg gctgactcaa
     2641 tcactggcgc tggatctggc ggagtacggc attacggtac attcactgat gctcggtaac
     2701 ctgctgaaat cgcggatgtt ccagtcactg ttgccacaat acgcgaccaa gctgggtatc
     2761 aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg cggctgcgat
     2821 tatcaagatg tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc gtactgcacc
     2881 ggacagtcga tcaatgtcac cggcggtcag gtgatgttct gatcaacagc ggagatccgt
     2941 taaggatctc cgtgagacta tagaatgcct gatgcgctac gctcatcagg catacaggac
     3001 ttccgccact acattaagga aaagttatgg tatccgcact catcaccgtc gccgttatcg
     3061 cctagtgtgc gcaactggcc ttaggcggct ggcaaatttc tcgttttaac cgtgccttcg
     3121 acacactatg ccagcaaggg cgggttggcg tgggccgttc cagcgggcgc tttaaaccgc
     3181 gggtcgtggt cgccatcgcg ctggacgatc agcagtgcat cgtcgacacc ttgtttatga
     3241 aaggactgac tgtcttcgcc cgaccgcaaa aaattcccgc aattaccggc atgcatgcgg
     3301 gtgatttaca gcccgatgtg atctttcccc atgatccact atcacagaat gctctatcat
     3361 tggcgcttaa actgaaacgt ggataatttc gttgtgaatg ttacttgctt gcgaagttat
     3421 cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt caggccgcca
     3481 ttctggagta tctgcaaaag cagggtaaat gctcggttga agaattggcg caatactttg
     3541 acaccacagg cacaaccatt cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca
     3601 ttcgtactta tggcggagtg gtgttgaata aagaggaatc cgatccgcct atcgatcata
     3661 aaacgctcat caacacccac aagaaagagc tgattgcaga agctgccgtt agttttatcc
     3721 atgatggcga ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg gttcccctgc
     3781 tctcgcgctt taataacatc acggtgatga ccaacagcct gcatatcgtc aatgcgctat
     3841 ccgaactgga taacgaacaa actatcctga tgccaggcgg aacatttcgc aaaaaatcgg
     3901 cctcatttca cgggcaactg gcagagaatg ccttcgagca tttcaccttc gataaattgt
     3961 ttatgggcac cgacggcatc gatctcaatg cgggcgtaac cacgttcaac gaggtttata
     4021 ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg gactcatcaa
     4081 agtttggccg taaaagcccc aacgtagttt gcagtcttga aagcgtcgat aagctaatta
     4141 ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg atcgatgtga
     4201 tcataaccgg agagagcaat gagtgaagca ctactgaaca cgggacgtca gacgttaatg
     4261 ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt tgttcgcgcc
     4321 gccaatatca tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc
     4381 cacattggta agaaaatcgc cgcaacactt gccagtaccg gcacaccggc tttttttgtc
     4441 catccggcag aagcactgca cggcgatctg gggatgattg aaagccgcga tgtgatgctg
     4501 tttatctctt actccggtgg cgcgaaggaa ctggatctga ttattccgcg tctggaagat
     4561 aaatctatcg cgctgctggc gatgaccggc aaaccgacgt caccgctggg cctggcggcg
     4621 aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc cgatgcacct tgcgccgacc
     4681 tccagcaccg tcaataccct gatgatgggt gacgcgctgg cgatggcggt catgcaggcg
     4741 cgcggtttta atgaagaaga ttttgcccgc tcccacccag ctggggcact gggcgctcgc
     4801 ttgctgaata aagtgcatca tctgatgcgt cgtgatgacg ctatcccaca ggtagcgtta
     4861 accgccagcg tgatggatgc gatgctggaa ctcagccgca ccggtctggg gctggtggcg
     4921 gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg acggcgattt acgtcgctgg
     4981 ctggtaggcg gcggcgcact caccacgcca gttaatgaag cgatgacgac aggcggcacc
     5041 acattacagg cacaaagtcg cgccatcgac gccaaagaag ttctgatgaa acgcaaaatc
     5101 actgccgcac cagtggtaga tgaaaacggc aaactcaccg gcgcaattaa cctgcaggat
     5161 ttctatcagg ccgggattat ttaatccttc aatcccagac gtttcgccag ccgatgcagg
     5221 ttggcgacgt cagtttccag cattcgcgca caggctgccc agttgtgatg attttgtgcc
     5281 agtgcctggc gaatagtttc acgctggaac gcttctgtcg cttcacgcag gttttgctta
     5341 acaacgagca ccgccgccgc ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt
     5401 tgcgcctcaa gaatcacttc atcgccgttg cgggtggctc tcgccagcac taccgcccga
     5461 tgaatagcat gttccagttc gcgcacgttt cccggaaaac tgtagtgttg cagtaaattt
     5521 cgcgccccgg cacttaatac cacgcgggag agcccctgcc gcaaacgaca ctgctcgcag
     5581 aaataccccg ccagcagaat gacatcatcg ccccgctcac gcagcggcgg caccgaaagt
     5641 ggaaacacgc tcaggcgatg aaacaaatcg gcgcggaatc gccctgccag cacctcttcg
     5701 cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga cccgcaaact acggtcatcg
     5761 ccaacgcgct gaatatcgcc atactgcaac accctcagca gcttggcctg caatgccaac
     5821 gacaactcgc cgatctcatc gagaaacaga gtaccgttat ccgccatttc aaacttcccg
     5881 ctgcggttac tgatagcgcc agtaaacgct cctttcacat gcccgaacaa ctcactttcc
     5941 gccacacttt ccggcagtgc ggcacagttg agatagacca gcggattcac cgcccgtggc
     6001 gaggcttcat gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc accgctgatc
     6061 agaacgttga gatcggacgc cgccacaatc tcaatctctt ttttcagttg cgtcatgcca
     6121 ggggacaagc caatcatctg cgtctgtttc accgcttcaa acggcgtggc atcgcctggc
     6181 agcatattct ggctttccag ttgttcaatc agcaacgcat tgcttaacgc tcccgccgcc
     6241 agcgcggcaa tcagccgtaa ctcttcgtcg ctgaaaacat cgaactgatc gggctgcatc
     6301 ccgtcgagcg tcagtgcgcc gatcaggttt tgtccgacaa acaatggcag accaacgcag
     6361 gcgtgaacct tcagactctc ctgcccagga atcaaaccgt catagggatc gggcaattcg
     6421 ctgtctgcgg gaaagcgcac cacatccccg gcgcgggcaa tcgcttccag ccgtggatgc
     6481 ccttccagcg caaagcgtct accgagtaca tcctttgcca gaccgtcgat ggcaagcgga
     6541 ataaactgcc gcgaatcgta acgtagcaac gcagacgcat cgcactccag cacctgacgt
     6601 agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg caattcgatg
     6661 gcgatattcg ccagcacatc aacggaaaaa ctcatctttg cctcactgtc aatttgacta
     6721 tagatattgt catatcgacc atttgattga tagtcatttt gactactcat taatgagcat
     6781 aattttattt atagagtaaa aacaatcaga taagaaactg gcacgcaatc tgcaattagc
     6841 aagacatctt tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa
     6901 ataacattca ttgggtgggt caacgtgact gggaagtgcg tgattttcac ggcacggaat
     6961 ataaaacgct gcgcggcagc agctacaaca gctacctcat ccgcgaagaa aaaaacgtgc
     7021 tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa
     7081 tcgatctggc ggatatcgat tacatcgtga ttaaccatgc agaagaggac cacgccgggg
     7141 cgctgaccga actgatggcg caaattcccg atacgccgat ctactgtact gccaacgcta
     7201 tcgactcgat aaatggtcat caccatcatc cggagtggaa ttttaatgtg gtgaaaactg
     7261 gcgacacgct ggatatcggc aacggcaaac agctcatttt tgtcgaaaca ccaatgctgc
     7321 actggccgga cagcatgatg acttacctga caggcgacgc ggtgctgttc agtaacgatg
     7381 ctttcggtca gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc
     7441 ttttcgagca gtgccagcgt tactacgcca atatcctgac gccgttcagc cgcctggtaa
     7501 cgccgaaaat taccgagatc ctgggcttta acttaccagt cgatatgata gcgacctccc
     7561 acggcgtggt ctggcgcgat aacccgacgc aaattgtcga gctgtacctg aagtgggccg
     7621 ctgattatca ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca
     7681 tgatggctga cgctatcgcc cagggaattg cggaaaccga cccacgcgtg gcggtgaaaa
     7741 ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac caatgtcttc cgctcaaaag
     7801 gcgtgctggt cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccgggctgg
     7861 tggaggagat gaccggatta cgcttccgta acaaacgcgc cagtgctttc ggatctcacg
     7921 gctggagcgg cggtgcgatg gatcgccttt ccacgcgcct gcaggatgcg ggtttcgaaa
     7981 tgtcgctcag cctgaaagcc aaatggcgac cagaccagga cgctctggag ttatgccgtg
     8041 aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga
     8101 atacggtagt taaagaagaa acctctgcca cgacgacggc tgacctcggc ccacgaatgc
     8161 agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg
     8221 cgccaggaac gccgtggagt gaagtcccgg ataacttcct ctgcccggaa tgctccctcg
     8281 gcaaagacgt ctttgacgaa ctggcatcgg aggcaaaatg agcaacggca ttgtgatcat
     8341 cggttcgggc ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccactat
     8401 tccattaacc ctgattgccg ccgacagcat agatgagtac aacaaacccg acctcagcca
     8461 tgttatcagt caggggcaac gtgccgacga ccttacccgc cagacggcgg gtgaatttgc
     8521 cgggcagttt aatctgcgcc tgtttccgca cacctgggtg acggatatcg atgccgaagc
     8581 ccatgtggtg aaaagccaga ataatcagtg gcaatacgac aagttagtgc tggcaaccgg
     8641 tgccagcgcc tttgtcccgc ctgtgcctgg gcgtgagtta atgctgacgt taaatagtca
     8701 gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt tgattgtcgg
     8761 cggtggtttg attggtagcg aactggcgat ggatttttgt cgtgcaggca aagcggtcac
     8821 gctaatcgac aacgctgcca gtattctggc gtcattaatg ccaccagaag taagcagccg
     8881 cttgcagcat cggttgacgg agatgggcgt tcatctgctg ttgaaatctc agttgcaggg
     8941 gctggaaaaa acggattctg gcattctggc aacgctggac caccagcgta gtatcgaagt
     9001 ggatgcggta attgccgcca ccggactgcg cccggaaaca gccctggcac gacgcgccgg
     9061 gctgacgatt aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgccgatat
     9121 ttacgcgctg ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc tgcagccgat
     9181 tcaacttagc gggatggtgc tggcaaaaaa tcttctcggc aataacacgc cgctgaaact
     9241 accggcgatg ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac
     9301 ccagcgtcag gatttacgct ggcagattaa taccgaacgc cagggaatgg tggcgcgcgg
     9361 cgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc
     9421 atttgtattg ttgaaaacgt tgccggtgta ggtgggctac tgtgcctaaa atgtcggatg
     9481 cgacgctggc gcgtcttatc cgacctacgg ggacgcatgt gtaggccgga taaggcattt
     9541 acgccgcatc cggcaagggt gtccaaatgc aacacgcttt atccgttctg gacttcaccc
     9601 gctaaccaac gcgccgcagc aataaccccc tgccccagag acaaaccgcc atcacccgcc
     9661 ggtaaactct gtggaaagag caatgtgaaa tcagcgagat aatgcgccag acgtgcacgc
     9721 agcaaacggt tatgaataac cccgccgcta aataccagcg tagtgatacc acgcatcgtg
     9781 gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg
     9841 cgttgattaa ccagtgcctg ccagttcagc cactgctgcc agaaagtggc gagatccagt
     9901 tgattgtcca cccgcggcat cgtcaccgga tgcgtcactc cgtcgcatga ggctgcgagc
     9961 gcctccagag cacaagccgc ttcaccttca taacttaacg tggctggcgc acagcccagt
    10021 gccgccgcca ctgcatcgaa caaccgccca cacgatgatg ccagcggcgc gttaattcca
    10081 cgctcaatgg cccgcgccag tacgctccag ttttgctgtt gcacacttgc cgtttcaggg
    10141 taattctgcc actccggcac aaagcgcagg cactgcgcca gcaggtttcg ccacggctgc
    10201 ttcgccgcca aatcgccacc cggaagcgcc actgcaggca agccgcccag gtgctcacat
    10261 tcgcgatagt tcacccgcag gcactcgccg ccccacaaag cgccattctc ccccataccg
    10321 ataccgtcga gcgtcaaagc aatgacatca ccgccatcca gcggccactg atgctctgcc
    10381 agacacgccg ctgcatgggc atgatgatgc agtaccgttt gcgtcggcag attcatttcg
    10441 ctcgcccact ggctggagac atagcccgga tgcacatcat gcacaatgta ttgcggagtg
    10501 aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc actgcatctg gatgccatcg
    10561 tcacttaaat cgcccagatg ctgactcaac accgcttgtt caccgcgcac caggcagaag
    10621 gtatttttca gatccgcgcc aagacacagc acaggcggaa catttttaaa gcccggaggc
    10681 aaaaccagcg catccggcac atacccccgc gaacggcgca gcatttcgcc gctttcgcgc
    10741 accactgaat catccatccg ctgcacgatg tcgcggttat gtagcaagaa tccgtcggca
    10801 atgccctgca aatccgccag cgcctgttcg ttgctgatag ctggtggttt accgctcagg
    10861 ttgccggagg tcatcaccag cgggcattgc agttcctgta acagcaaatg ctggagcggg
    10921 ttcgcaggca acatcacccc gacttcgtta aggccagggg cgatatcatc acaaagctca
    10981 ggtacgtatt ttttatccac cagcacaatc ggcgcagcgg gcgtggtaag caactggcgc
    11041 gcagcgtctg gtaaactttc tgccactggc aacatgactg ccagcggttt cgccgggcga
    11101 tgtttgcgct cccgcagtgt cgccaccgcg ttactgttac gtgcatcgca ggcaagatga
    11161 aatccgccaa tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagctgcc
    11221 tgtaatgccg cctcttgttc tgcatgttca ccatgactta cccattcaag atgcgggcca
    11281 cactccgggc aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc gcggtactct
    11341 ttgtcacagg ccggacatag cggaaacgcc gccatcacgg taaacgggcg gtcgtaaggc
    11401 atcgcgcgaa taatggtgaa acgcgggccg cagtgggtac agttgataaa cggataacga
    11461 taacgccgct cgcctggggt attcatttcg gcaaggcaag cagggcaagt ggcggcatcc
    11521 gggacaattt gcgtattcat ggtgccgccg gtgctctggc ggatggtgaa ctcggtgggc
    11581 agttgtgacc agataaacgg ctcacgctcg acgctatcaa tacgcgccag tggcggacag
    11641 tgctgatgca attgaacaag aaacgtttcc gggtcttcca gcagccggac ttctacgcca
    11701 tcgccgtcat tacatacatc gccgtgaaga tttaattgct gtgccagctg ccagacaaac
    11761 ggacgaaaac cgacgccctg cactttgcca cgaatacgca gttggacacc gcaagatgtg
    11821 ttttttgcca ttgagttatt cccgccatca tgaattgcgt aacccgccct gccggacacg
    11881 acagcgtcgt atccggcagt ctcaggtcgg cgatcccgcc cgctccgtat tctacgaata
    11941 tttccgggaa ttcctttgat gccagaacag ttctgtaaga tttttagaac atcagcgccg
    12001 tacggcggcg tttttctgcg ctcagttgtt caagtttatt acgatcgaca caaatcagcg
    12061 catgggtcgg gcaagccgcc atacacgccg ggccgtcttc acgatggttg cacaggtcgc
    12121 atttattggc ttcggctttg tcagcccgta cattcagacc cgcgccgctg ttgcgaatca
    12181 caggacgtac caccacttcc atcgcgccat acgggcaagc caccacgcag gttttgcaac
    12241 caatgcaacg ttcctgcatc acaggaacaa accctttatc acggctgata gcaccattcg
    12301 ggcagacgtt agcgcacggt gcatcttcac actgacggca aactgtcgcc gtggaaatgt
    12361 tcacaccttt aatgacatgg atacgcggta aaaatgtttc cggggtcagc gatgcacagt
    12421 cctgattttc ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt
    12481 tactcgcgtc agcaatgatg aaacggttca tcaaattctc cagcaatgac agttaatgcg
    12541 ccgatacatt cacaaatcat gccagttttt aatttgctgt tatttaagga aattaatttc
    12601 tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt
    12661 tatcgcgaaa gggcaatgag tgagtcgcgg cggatcagtt ttccgctaaa ggttttcggc
    12721 ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc ctgaatcatc
    12781 tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc aattgcgata
    12841 tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc gtgcaacgct
    12901 ttgatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa tttagcccct
    12961 cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt accgttagcg
    13021 ataagttttt cattgagcgc aataccatgc tgcgacagcg cgtctttata cccggcaaga
    13081 cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc ctgatgcccg
    13141 gcgtttatca actctgccac ggcgttaaag ctggtctgtt tatgatcgca ccagacgcta
    13201 tggctgctat tattgcgcag gcggcgatta agcaccatta tcggctgact gtgcgcgtca
    13261 atgatgtcat caatctcatc cacgcttaaa aaacgcgggt aaatcataat ggcgtcgcag
    13321 cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg tttaccatct
    13381 gccaatagca actgtcgccc tttctcttcc gccattcgcg cggcatgaaa cagtaattca
    13441 ctaaaataaa tgccgtgata aagcgtgttg gtcactacca gccccagcgt ctgagtactc
    13501 ttcgccgaca gattgcgggc cagcaaattt gggcggtaac tgctctcttc taccgcctga
    13561 aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac gcgggaaacg
    13621 gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat cattttcatc
    13681 cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt gcgcaatcgg
    13741 ctccagcgga taagacgagg tcgccgtgcc ggttttgatg acttttttga taaaggtaaa
    13801 catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc
    13861 tttgtacggc accactttgc tcttcttctt acgcacatcg actacggtca tgcggtcggt
    13921 acaggagtag caagggtcca ggctaccgat aatcagcggc gcatcggaaa cggtattgcc
    13981 acgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc agcgccagcg
    14041 gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc gcggcgcttc
    14101 ggcaaagccc agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca tcagcggacc
    14161 gcccggcagg ttatccagac cgtagtcgat catattcagc gcggtataga cctcgttgat
    14221 tcgcactttc agacgggaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag
    14281 caggccataa ccgacaaacg ggtgatcggc gcgagtatcg cgggcgagac cgctggcgcg
    14341 aaccatcggg ccaacgttac tgaaatcgcg ggcgatttcc gggtccagac gaccaatgcc
    14401 gacggtgcgt tgttccatgt tcggcgtact cagcagcaca tccaccagct cctgcacttc
    14461 acgacgcatt tgttgcgcca gctggcgggt ctggatcatg tcatctttca gcagatcgcg
    14521 acgaatcccg ccgatcaggt tcaggccgta ggttttacgc gcaccggtaa ggatctctgc
    14581 cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt cgaagccggt
    14641 aaagtgacag gcgaggccga ggttgagcag atgcgggtgc aggcgttcca cctccagcag
    14701 aatggcgcgg atcatctgcg cgcgttctgg cacctgaata cccatcgcgt tttccaccga
    14761 cgtggtgtag gcggtgctgt gggcaaagcc gcagatcccg cacacacggt cagagaggaa
    14821 ggtcacttcg ttataaccca tgcgggtttc cgccagtttt tccataccgc gatggacgta
    14881 gaacagacgg tagtcggcgt cgataatgtt ttcgccatcg acgaacagac ggaagtgacc
    14941 cggttcgtca gaagtgacgt gcagcggacc aatcggcacg acgttgtttt tcttgtcgcc
    15001 cagttcgttg atgaactcgt aggtttcagc atcggtagtc ggtgccggac gctgacgata
    15061 atccatgctg tctttacgca gcggataaag ttcatccggc cagtcatccg gcagcaccag
    15121 acgacgttca tccggcagac caaccggaat caaaccgtac atatcgcgca cttcgcgctc
    15181 gccccacacc gccgccggaa cgcgcggcgt cacggacgga tactccggtt tgttggcgtc
    15241 aacttcgacg cgaaccgtta cccaacactt ggtgcccttc tccatcgaca gcacgtaata
    15301 aacggcgtaa tgaccattca gtttgcgttc gtcgttacca aacagcaccg acagccagcc
    15361 accctgcttg tagtagagaa actccaccac ttccggcaga tagttcacct ttacggtgac
    15421 agtcagctga tctttggtct gccaggcgtg gtccagcacg acgcctagaa atgcctcatt
    15481 cagcgcggcg agataatgtt gacctaattt ttcttcagac atgctcaaac tctctttaat
    15541 cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc caaacccagc ccaggtaatg
    15601 cgcggagtaa tatcaagacg cagacgggcc atgctgtttt cgaacagcgc gataaccagg
    15661 acgccgacca ccagtttgac gatggcaatc accagcgcca gcagcagtcc accgacggtg
    15721 aaggtttcca tttgtcccca cggaataaac accccgacga acatctgcaa caccaccagc
    15781 tgtttcaggc tgatacccca tttcatgacg ccaaagccgc taccgctgta ttcggagagc
    15841 ggaccttcct gcaactcctg ctcggcttcc gccaggtcga acggcagttt gcccatttcg
    15901 ataaaagtgg cgaacgcaca ggcgcagagc gccagcacca gcgggatgct ttggctcagc
    15961 ggccagtgat aaacggtgtc ggtaatgttg ctgatgttgg tggaaccggc aacctgtgcg
    16021 gcaacccaca gaccaagcag caacatcggt tcgaccagca cgccaagcat cgcttcacgg
    16081 ctcgcaccga tagcggtaaa cgggctaccg gtatccagac cggaaatggc aaagaagaaa
    16141 cgcgcgatgg cgaagagata cagtaaggtg atcaaatcgc ccagttgcgg cagcggagaa
    16201 ccgacggtca ccaccggcag cgcagtagcg atagtcagca tgacgcccac catcacatac
    16261 ggcgtcaggc ggaacatcca gccagaggca tccgggccaa cgctctggcg gcccagcagt
    16321 ttgataatgt cgcgatactc ctgcaacacg cccggcccgc gacggttatg cagacgggcg
    16381 cgcgccacgc gggtaatacc ggagagcagc ggcgcaacgg caaataacac cagcgcctga
    16441 attaacggat ataaaacact cattctcagg ctcctcgtga aacaataatc accaccagta
    16501 ccgccagttc aaccagcgcc atccggcgga acagcaacgc actaccttcg cactgccagc
    16561 ccggcactag cgacaccgga ttcagccatt tgcgcagttt cagcactggc gcaaacgcct
    16621 gtttaaccgg catggcaaaa ccgtgagcgg taatcaccat tgatttttca tggtcgtagc
    16681 cgcacaccca ggccgcaccg cgggaacgtg acggcaggcg gtcgcctttg caaatcgcca
    16741 taatgatgaa tggcagcagc gggcaggcaa tcagcagcaa tgtgatcatt ggttgagaaa
    16801 cggtggtgtt agcaggctcc agaggcagag gtacagcagc agagagcatc ggcagcagcc
    16861 acggcgcagc aacaccgcca attacgcagc aaatcgccag tgccactacg cttacgctca
    16921 tcaggagcgg cgcacaggtg gcgttttcgg cttctttggt acgcggcgcg ccgaggaacg
    16981 tgacgccata gactttcgcc atacacatca ccgccagcgc accggtaatt gccagaccca
    17041 cagcgagcag cggccccagc agacgggcaa caaacgcgcc gctattgctc agtttgaaga
    17101 aggattgata gataacccat tccccggcaa acccgttcag tggcggcagc gcagccattg
    17161 ccatcaaccc gactaacatg gcgatggaga taaccggcat tttcttgcca ataccaccga
    17221 gtttttcgat atcgcgatga ccggtacgga accagacgct ccccgccccg aggaacagta
    17281 cgcttttgaa caggctatgg ttaagcagat ggtacagacc accgaccagg ccaagagcaa
    17341 tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc gcccagcccc agccccagcc
    17401 ccagccccag caggataatg ccgatatttt ccagggtgtg gtaagccagc aggcgctgga
    17461 tgttgtgttc catcaacgca tacagaccac cgacgaacgc ggtgatcatg ccgagcacca
    17521 gcagcgcgat cccccaccac agcggtgcat taccgcccag cagtgacagg gttaacatgc
    17581 ccagcaggcc aattttcatg actaccgtag aaaacaacgc ggcagctggc gcagaggcgt
    17641 tagcatgtgc ctgcggcacc cagccgtgca gcggaataat cccagccagc aggccaaagc
    17701 caatcacacc gagcagccag atatcggaac cgagcggcag ctgttgcata cgcatatcca
    17761 gcaggcgcag atccagcgtg ccataacgct gccacagcag ccagcaagca atcaccagca
    17821 gcagagtgcc aagacgcccc agcgcaaacc acagtttgcc ctctttgctg ttgctggtga
    17881 ggaacaccgc acacaaggcc atgacttcgg ccattaccac gaacatgccg aggttgctgg
    17941 cgatgacggc gcagacggcg gcagccatca acatattgat ctgcaagccg ttgcacttca
    18001 cctgcgcgtg gcgatgccag tcaatgttat agaggctgac aaacagaccg caaaggccga
    18061 gggtaatcag ccaaatcgcg ttaagcgggg agatttgcac atcgtagctt accagcgaca
    18121 gcgcaccgct cacgccaacc gcgccagtca gtacagtgaa gcccgcggct gccgtataca
    18181 gactaccaac cgcgccgcca attccggcta tccagccact tagcgctttt tgaaaagaga
    18241 aatgccagaa cagcggcggc gacaaaccat gccacgccgc tattgatcag ggaaattgcg
    18301 ctcatttagc ctctccactt tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt
    18361 taaaggtcag ctcacgctta cgtttgctga cgcgggcgat atcggtgtta tccaccagat
    18421 gcagggcttt agtcgggcac atccgcacac aggccggacc ttgttcatca aagctacaaa
    18481 ggtcacattt gacggcgatc gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac
    18541 gcgccggagc aggcggtgcc ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca
    18601 gcggacggct gccggaaaat tcaattgcgc caaacgggca ggcgatgccg cacagtttgc
    18661 agcttacgca cagactttca ttcaactgca cggccccatc aacgcgggtg atggcgttaa
    18721 ccgggcagac caccgcgcag ggtgcatctt cacagtggtg acagagctgc ggcgcagatt
    18781 ctttttcatt gagcatcact ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg
    18841 tctctgaaca ggcggcctca caggtgtggc agccgataca gagcgtggag tcggcaatta
    18901 caaaacgatt caccaggcat tcctcaggtg attgtcattt ttgacgaaaa catgccgatg
    18961 aaatgtcatt ttcgacactc atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa
    19021 acgggcaaag cctcagctca tgctgccggg ctttgtccct ttaccagttg gcttaaattc
    19081 accggcacat tgttttcaat ggcggtgtat aagctgtcgt aaacgccagc gattttttcg
    19141 agatagtgtt ccagaacctg ttcgcgatcg cggatgttga ccttgccatc aatcatgccg
    19201 tcaatgctca gttgcgcctg cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg
    19261 taatactcga tggtgtggct gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt
    19321 tcaaaaagga cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg atacaggcgc
    19381 gctttcgata acgtgatccc ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt
    19441 aggcgacgat gccgctgtgc gatgtaatcg gctttctcgc ttatttccca aatagtcatt
    19501 gtcaggttac tcgtttaaca gagatgccag ctttaagcat tttttgtgcc aacttttaat
    19561 ttattgttat taaagagatt tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc
    19621 atttcgacat catcgacatt attcaccgca gggataatca acactggcgc aattattgct
    19681 tgtagctggc aatagttaat gggaggcgat atgcacgaaa taaccctctg ccaacgggca
    19741 ctggaattga tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg
    19801 ctcaaaattg gcgcattttc ttgtgtcgaa accagctctc ttgccttttg ttttgatctg
    19861 gtttgccgcg gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa
    19921 tgctggtgtg aaacatgcca acagtatgtg acgctactga cccagcgcgt ccgccgctgt
    19981 ccacagtgtc atggtgacat gctacagatt gtggcagacg acggtttaca gattcggcgg
    20041 atagaaatag accaggagtg agcgatgtgt acaacatgcg gttgcggtga aggcaacctg
    20101 tatatcgagg gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc atttgccccg
    20161 gcggcacgcc cgcagatgaa aatcaccggc attaaagcgc ctgaatttac ccccagccag
    20221 actgaagaag gcgacctgca ttacggtcat ggcgaagcgg gcactcacac accgggcatg
    20281 agccagcgtc ggatgctgga agtcgaaatt gacgtgctgg acaaaaacaa ccgtctggct
    20341 gaacgcaacc gcgcgcgctt tgctgcccgc aagcaactgg tgctcaacct ggtttccagc
    20401 cctggttccg gtaaaaccac cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt
    20461 ccgtgcgcgg ttattgaagg cgaccagcaa accgtgaacg atgccgcacg cattcgcgct
    20521 accggtacac cagcgattca ggtgaacacc ggtaaaggct gccatcttga cgcacagatg
    20581 attgccgacg ccgcaccgcg tctgccactg gacgataacg gtattctgtt tatcgaaaac
    20641 gttggcaacc tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa agtagcggtg
    20701 ctttccgtta ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc
    20761 tcgctgatgc tgctcaacaa agttgacctg ttgccgtatc tcaactttga cgttgagaag
    20821 tgcatcgcct gcgcccgcga agtcaatcca gaaattgaaa tcatccttat ttccgccacc
    20881 agcggcgaag ggatggacca gtggctgaac tggctggaga cacagcgatg tgcataggcg
    20941 ttcccggcca gatccgcacc attgacggca accaggcgaa agtcgacgtc tgcggcattc
    21001 agcgcgatgt cgatttaacg ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg
    21061 gccagtgggt actggttcac gttggctttg ccatgagcgt aattaatgaa gccgaagcac
    21121 gcgacactct cgacgcctta caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt
    21181 tgtatggcga ggaaaaataa tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat
    21241 gcagttaatt gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc
    21301 tctgcggatt atggaagtgt gtggcggtca tacccacgcc atttttaaat tcggcctcga
    21361 ccagttactg cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact
    21421 gccgatgggt agaatcgaca cctgcgtgga gattgccagc catccggaag tcatcttctg
    21481 tacctttggc gacgccatgc gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc
    21541 acgcggtgcc gatgtgcgca tcgtttactc gccgatggat gcgttgaaac tggcgcagga
    21601 gaatccaacc cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca tgccgaccac
    21661 cgccatcact ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact tcttctgcca
    21721 gcacattacg cttatcccga cgctgcgcag tttgctggaa cagccggata acggtatcga
    21781 cgcgttcctc gcgccgggcc acgttagtat ggttatcggc actgatgcct ataattttat
    21841 cgccagcgat tttcatcgtc cgctggtggt cgctggtttc gaaccccttg atctactgca
    21901 aggcgtggtc atgctggtgg agcagaaaat agcggcccac agcaaggtag agaatcagta
    21961 tcgtcgggtg gtaccggatg ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt
    22021 ctgtgtcaac ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca
    22081 cctgacgccg gattatcaac gattcgatgc cgaagcacat ttccgcccgg caccgcagca
    22141 ggtctgcgat gacccgcgcg cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca
    22201 tcaatgcccg ctgtttggta acacctgtaa tcctcaaacc gcatttggtg cactgatggt
    22261 ctcctccgaa ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg
    22321 aataatatcc aactcgccca cggcagcggc ggccaggcga tgcagcaatt aatcaacagc
    22381 ctgtttatgg aagcctttgc caacccgtgg ctggcagagc aggaagatca ggcacgtctt
    22441 gatctggcgc agctggtagc ggaaggcgac cgtctggcgt tctccaccga cagttacgtc
    22501 attgacccgc tgttctttcc tggcggtaat atcggcaagc tggcgatttg cggcaccgcg
    22561 aatgacgttg cgggcagtgg cgctattccg cgctatctct cctgtggctt tatcctcgaa
    22621 gaaggattgc cgatggagac actgaaagcc gtagtgacca gcatggcaga aaccgcccgc
    22681 acggcaggca ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg cgcggcagat
    22741 aaactgttta tcaacaccgc tggcatgggc gcaattccgg cgaatattca ctggggcgca
    22801 cagacgctaa ccgcaggcga tgtattgctg gttagcggta cactcggcga ccacggggcg
    22861 actatcctta acctgcgtga gcagctgggg ctggatggcg aactggtcag cgactgcgcg
    22921 gtgctgacgc cgcttattca gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat
    22981 gccacccgtg gtggtgtaaa cgcggtggtt catgagttcg cggcagcctg cggttgtggt
    23041 attgaaattt ctgaatcagc gctgccggtt aaacctgccg tgcgcggcgt ttgcgaattg
    23101 ctgggactgg acgccctgaa ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc
    23161 aacgcggcag agcaagtgct ggcagcgtta cattcccatc cactggggaa agacgcggcg
    23221 ctgattggtg aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa
    23281 cgaaccctcg atttaccaca cgccgaaccg cttccgcgta tatgctaata aaattctaaa
    23341 tctcctatag ttagtcaatg accttttgca ccgctttgcg gtgctttcct ggaagaacaa
    23401 aatgtcatat acaccgatga gtgatctcgg acagcaaggg ttgttcgaca tcactcggac
    23461 actattgcag cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg
    23521 ttctgcgctc gccgacaacg cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc
    23581 ttattacgcg tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa ctgttctggc
    23641 acacggtccg gtacgcagca ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga
    23701 attttgtgaa acctggccgc agctggccgc aggtgggcta taccccaaat ttggtcacta
    23761 ttgcctgatg ccactggcgg cgggagggca tatttttggt ggctgtgaat ttattcgtta
    23821 tgacgagcgt ccctggagcg aaaaagagtt caatcgtctg caaacattta cgcagatcgt
    23881 ttctgtcgtc accgaacaaa tccagagccg cgtcgtcaac aatgtcgact atgagttgtt
    23941 atgccgggag cgcgataact tccgcatcct ggttgccatt accaacgcgg tgctttcccg
    24001 cctggatatg gacgaactgg tcagcgaagt cgccaaagaa atccattact atttcgacat
    24061 tgacgatatc agcattgtct tacgcagcca ccgtaaaaac aaactcaaca tctactccac
    24121 tcactatctt gataaacaac atcccgccca cgaacagagc gaagtcgatg aagccggaac
    24181 cctcaccgaa cgcgtgttca aaagtaaaga gatgctgctg atcaatctct acgagcggga
    24241 cgatttagcc ccctatgaac gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt
    24301 gtgcctgtta ccgctgatgt ctggcgacac catgctgggc gtgctgaaac tggcgcaatg
    24361 cgaagagaaa gtgtttacca ctaccaatct gaatttgctg cgccagattg ccgaacgtgt
    24421 ggcaatcgct gtcgataacg ccctcgccta tcaggaaatc catcgtctga aagaacggct
    24481 ggttgatgaa aacctcgccc tgaccgagca gctcaacaat gttgatagtg aatttggcga
    24541 gattattggc cgcagcgaag ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca
    24601 aagtgacagt accgtgctga tcctcggtga aactggctcg ggtaaagagc tgattgcccg
    24661 tgcgatccat aatctcagtg ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc
    24721 gatgcctgcc ggattgctgg aaagcgatct gtttggtcat gagcgtgggg cttttaccgg
    24781 tgccagcgcc cagcgcattg gtcgttttga actggcggat aaaagctctc tgttcctcga
    24841 cgaagtgggc gatatgccac tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca
    24901 ggagtttgaa cgtctcggca gcaacaaaat cattcagacg gacgtacgtt taattgccgc
    24961 gactaaccgc gatctgaaaa aaatggtcgc cgaccgagag ttccgtagcg atctctatta
    25021 ccgcctgaac gtattcccga ttcacctgcc gccactacgc gagcgtccgg aagatattcc
    25081 gctgctggcg aaagccttta ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag
    25141 cattcctgcc gagacgctgc gcaccttgag caacatggag tggccgggta acgtacgcga
    25201 actggaaaac gtcattgagc gcgcagtatt gctaacacgc ggcaacgtgc tgcagctgtc
    25261 attgccagat attgctttac cggagcctga aacgccgcct gccgcaacgg ttgtcgccca
    25321 ggagggcgaa gatgaatatc agttgattgt gcgcgtgctg aaagaaacca acagcgtggt
    25381 tgccgggcct aaaggcgctg cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg
    25441 gatgaagcgg ctgggaattg ataaatcggc attgatttaa ctgcaaattg ccggacagat
    25501 ctgcctgtcc ggcatactat tcacgaggtt ttttcggacg atatttttcc ggcagttctg
    25561 gcaccggacg cttgtcatcg atgagatgac gcacggttaa gatcggatga cgccacagca
    25621 ttctcggccc ggcccaacgc ataatctgtt tcatctcttc acgctttgca ggctggtaac
    25681 agtgcaccgg acactgctta caggctggtt tctcttcgcc gaacacacat ttatccagcc
    25741 gcttttgcgc gtaaacaaac aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc
    25801 atttcgcttg ataaagatcg atcatttttt taatcgtcag tttttcacga gagatacgct
    25861 tgccggacat gctgcctcca cctcattaag atgcatttat att
//
LOCUS       DAJTUN010000051        25562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTUN010000051 DAJTUN010000000
VERSION     DAJTUN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.051"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(1..1998)
                     /gene="yccS"
                     /locus_tag="OO470_001969"
                     /pseudo
     CDS             complement(1..1998)
                     /gene="yccS"
                     /locus_tag="OO470_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
     gene            complement(2008..2454)
                     /locus_tag="OO470_001970"
     CDS             complement(2008..2454)
                     /locus_tag="OO470_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6251224.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2577..4631
                     /gene="helD"
                     /locus_tag="OO470_001971"
     CDS             2577..4631
                     /gene="helD"
                     /locus_tag="OO470_001971"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6251225.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4663..5121)
                     /gene="mgsA"
                     /locus_tag="OO470_001972"
     CDS             complement(4663..5121)
                     /gene="mgsA"
                     /locus_tag="OO470_001972"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6251226.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5217..5879)
                     /locus_tag="OO470_001973"
     CDS             complement(5217..5879)
                     /locus_tag="OO470_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6251227.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6052..6465
                     /locus_tag="OO470_001974"
     CDS             6052..6465
                     /locus_tag="OO470_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6251228.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6510..6827)
                     /gene="hspQ"
                     /locus_tag="OO470_001975"
     CDS             complement(6510..6827)
                     /gene="hspQ"
                     /locus_tag="OO470_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6251229.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6885..8075)
                     /gene="rlmI"
                     /locus_tag="OO470_001976"
     CDS             complement(6885..8075)
                     /gene="rlmI"
                     /locus_tag="OO470_001976"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6251230.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8170..8448
                     /gene="yccX"
                     /locus_tag="OO470_001977"
     CDS             8170..8448
                     /gene="yccX"
                     /locus_tag="OO470_001977"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6251231.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8445..8774)
                     /gene="tusE"
                     /locus_tag="OO470_001978"
     CDS             complement(8445..8774)
                     /gene="tusE"
                     /locus_tag="OO470_001978"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6251232.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8865..9524)
                     /gene="yccA"
                     /locus_tag="OO470_001979"
     CDS             complement(8865..9524)
                     /gene="yccA"
                     /locus_tag="OO470_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6251233.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9731..9818)
                     /locus_tag="OO470_001980"
     tRNA            complement(9731..9818)
                     /locus_tag="OO470_001980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9782..9784),aa:Ser,seq:tga)
     gene            10244..11362
                     /gene="hyaA"
                     /locus_tag="OO470_001981"
     CDS             10244..11362
                     /gene="hyaA"
                     /locus_tag="OO470_001981"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6251234.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11359..13152
                     /gene="hyaB"
                     /locus_tag="OO470_001982"
     CDS             11359..13152
                     /gene="hyaB"
                     /locus_tag="OO470_001982"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6251235.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13171..13878
                     /gene="hyaC"
                     /locus_tag="OO470_001983"
     CDS             13171..13878
                     /gene="hyaC"
                     /locus_tag="OO470_001983"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6251236.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13875..14462
                     /locus_tag="OO470_001984"
     CDS             13875..14462
                     /locus_tag="OO470_001984"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6251237.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14459..14857
                     /gene="hyaE"
                     /locus_tag="OO470_001985"
     CDS             14459..14857
                     /gene="hyaE"
                     /locus_tag="OO470_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6251238.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14854..15711
                     /locus_tag="OO470_001986"
     CDS             14854..15711
                     /locus_tag="OO470_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6251239.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15845..17389
                     /gene="appC"
                     /locus_tag="OO470_001987"
     CDS             15845..17389
                     /gene="appC"
                     /locus_tag="OO470_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6251240.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17401..18537
                     /gene="appB"
                     /locus_tag="OO470_001988"
     CDS             17401..18537
                     /gene="appB"
                     /locus_tag="OO470_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6251241.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18549..18641
                     /gene="cbdX"
                     /locus_tag="OO470_001989"
     CDS             18549..18641
                     /gene="cbdX"
                     /locus_tag="OO470_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6251242.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18721..20019
                     /gene="appA"
                     /locus_tag="OO470_001990"
     CDS             18721..20019
                     /gene="appA"
                     /locus_tag="OO470_001990"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6251243.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20134..22315)
                     /gene="etk"
                     /locus_tag="OO470_001991"
                     /pseudo
     CDS             complement(20134..22315)
                     /gene="etk"
                     /locus_tag="OO470_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22335..22781)
                     /gene="etp"
                     /locus_tag="OO470_001992"
                     /gene_synonym="yccY"
     CDS             complement(22335..22781)
                     /gene="etp"
                     /locus_tag="OO470_001992"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6251244.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22769..23908)
                     /locus_tag="OO470_001993"
     CDS             complement(22769..23908)
                     /locus_tag="OO470_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6251245.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(23954..25114)
                     /locus_tag="OO470_001994"
                     /pseudo
     CDS             complement(23954..25114)
                     /locus_tag="OO470_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25193..>25562)
                     /locus_tag="OO470_001995"
     CDS             complement(25193..>25562)
                     /locus_tag="OO470_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6251246.1"
                     /translation="TGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDP
                     LSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDS
                     MAARYRDSRGREWDKIEIDK"
BASE COUNT         6113 a         6516 c         6785 g         6148 t
ORIGIN      
        1 atgcctgatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
       61 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
      121 cgaggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa acgcggcttc
      181 gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg acgccagttc
      241 agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc
      301 ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg tggcgcgttc
      361 gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca ctgccgccca
      421 cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt caaaaccttc
      481 acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgagcgta
      541 ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca ccagctgccc
      601 ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta ccagcgtacc
      661 aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa
      721 acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga tggcgtagcc
      781 gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt ccggcgtgaa
      841 gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat cagcgagctc
      901 attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg ttgccagttg
      961 ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta aatcggcggg
     1021 tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa
     1081 atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt gctggcacgc
     1141 ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg
     1201 ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa tatcctgtgc
     1261 gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg
     1321 ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt tggcgagagc
     1381 caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt
     1441 gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg
     1501 gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg caccggccag
     1561 cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat
     1621 ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa
     1681 aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag
     1741 caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa
     1801 acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc ccagcgttag
     1861 cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc
     1921 aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg
     1981 acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcacg
     2041 cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata gtgcaatagc
     2101 ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac attgtgcgat
     2161 gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt
     2221 caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca gttcatcgac
     2281 atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg
     2341 tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca gccagcccag
     2401 agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc
     2461 tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca
     2521 cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg
     2581 aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga
     2641 tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaatc
     2701 aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa tttgtactgc
     2761 cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag cgtttttacc
     2821 atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg
     2881 ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa tggctgacgc
     2941 gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc
     3001 cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa tgtcaggctt
     3061 ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc gaagccatgc
     3121 ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat ccggcgcagg
     3181 cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca ggaagcggaa
     3241 aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg
     3301 agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac gagcggattc
     3361 gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg gcgctgcata
     3421 ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat gataccgctg
     3481 cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg
     3541 cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact
     3601 tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc tgggtaagtc
     3661 tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc gaagagattc
     3721 gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc tggaaaggtg
     3781 cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg attgtgattc
     3841 tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa tttcaggata
     3901 tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt
     3961 tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc
     4021 tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta gacacgactt
     4081 accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag aacccaggcc
     4141 agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc acgttattgg
     4201 atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa ccggaagagc
     4261 gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa aaagcggcaa
     4321 cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa gggcaacagg
     4381 cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg gctgcggcgc
     4441 gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg gacgctgaag
     4501 aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg gcactgttta
     4561 acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga
     4621 gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca gacggtcagc
     4681 gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat gcggcgactg
     4741 gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata ccgtcgccag
     4801 acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat cccagaagaa
     4861 tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt caccccccat
     4921 tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca agttaccggt
     4981 agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt ccacccagct
     5041 catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa
     5101 agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca gattactgta
     5161 agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag
     5221 gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt
     5281 ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg aatagcatcc
     5341 agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc aaattgcggt
     5401 agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata atcaatcgtt
     5461 tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt cgcatcgcca
     5521 tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc attctccagg
     5581 cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac caccagcggt
     5641 ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac
     5701 actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag cagagagctg
     5761 gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg
     5821 gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc ggttttcata
     5881 ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc tttaatcata
     5941 cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat tagctgcatg
     6001 gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat aatgaaagaa
     6061 accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg ggcaagcgac
     6121 aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc
     6181 attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg gtatggcacg
     6241 ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc agaagcggcg
     6301 tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat gcaactgggg
     6361 gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt ggtgatggat
     6421 cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat ccccggaagg
     6481 caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc ctggagttgt
     6541 ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc atcctgcaat
     6601 tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc
     6661 atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag ctcatcaggc
     6721 gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag ataacctaac
     6781 agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt cacctcccgg
     6841 gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat gacgcgacag
     6901 gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg atcggctgcc
     6961 tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc atccgcgatg
     7021 attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag gagaataccg
     7081 ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg acacgcgccc
     7141 atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac gtcaaatttt
     7201 tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac aaactcagcc
     7261 ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag cgcttcctgg
     7321 gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca
     7381 ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg ggtagccagg
     7441 cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat atccaccagc
     7501 agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc ggtgacgagg
     7561 ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc
     7621 gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg atattctgcg
     7681 ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt aatacccggc
     7741 aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt ttgcgccagc
     7801 cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac
     7861 tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga ataagcgccg
     7921 cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag gctggcttta
     7981 ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag taatgatttt
     8041 tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt cgatgcaaag
     8101 aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt aaggataagg
     8161 agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt tcagggcgta
     8221 ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg gtacgccaaa
     8281 aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca ggtagaaaaa
     8341 ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc
     8401 gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat acattttacc
     8461 ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg gaacagtcgg
     8521 tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc ttttaccagc
     8581 atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa acgcaccact
     8641 tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac tgccagcggt
     8701 tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat ctctttacct
     8761 tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta acaaaaaaca
     8821 aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc gcggctggcg
     8881 aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata caggctaacc
     8941 gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc aaacaagata
     9001 gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc
     9061 aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat cagcataccg
     9121 ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca gaagaacact
     9181 aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga cagataggtg
     9241 ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc ggagataatc
     9301 ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat acccaccaga
     9361 gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc
     9421 gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt atgggtgcta
     9481 agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc actctctatg
     9541 acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag aagttttacc
     9601 catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc attaagcaaa
     9661 tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt agctaacccc
     9721 ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt
     9781 ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca
     9841 tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc
     9901 attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc cttctctttt
     9961 actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat aggtccattt
    10021 ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt ttcactacgc
    10081 tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct ttcgttttca
    10141 gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc gaccataagc
    10201 gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata acgaggaaac
    10261 attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca aatattgtag
    10321 tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg cctgggcgct
    10381 ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta cctgctgtac
    10441 cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt ccctgatttc
    10501 cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag aagtctttga
    10561 agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta atccgccgct
    10621 gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa aactcaaacg
    10681 tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct ggggctgcgt
    10741 gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca tcaccgacaa
    10801 acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg ccatcattac
    10861 ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc cactgatgtt
    10921 ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg ccggagagtt
    10981 cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca aaatgggctg
    11041 caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg gcgtttcttt
    11101 cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct gggatcgcgg
    11161 ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca ccgccgatac
    11221 cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg cagttgccag
    11281 cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac atcagccagg
    11341 caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac catcaataat
    11401 gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat gcgctgtgaa
    11461 gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac catgtttcgc
    11521 gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt cgttgaacgt
    11581 atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat cgaagatgcc
    11641 atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct ggcaacgctc
    11701 tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg gatcgacgtg
    11761 ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag tctctcctct
    11821 tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa gaaattcgtt
    11881 gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca gtacaaactg
    11941 ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga tttccaacgt
    12001 gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa ctggattgtc
    12061 ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc ggtcaatatg
    12121 gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat taacaacgtg
    12181 atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga aatcggcacg
    12241 ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc caacgacttt
    12301 ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt caacaatgtg
    12361 ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca cgcctggtat
    12421 cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga cccgtggtac
    12481 aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga acaggaacgc
    12541 tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg gccgctggca
    12601 cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt cgatcgcatg
    12661 atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg cattttgtgc
    12721 cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga caagctgatg
    12781 accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga acctgcaacc
    12841 tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc gttaggccac
    12901 tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc gaccacctgg
    12961 aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc gctgatgaac
    13021 accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca cagctttgat
    13081 ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct gatctccgtg
    13141 caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa cgttgtcagc
    13201 cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt atgcatggcg
    13261 gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag tggcgaggcg
    13321 acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg gatgattttt
    13381 accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta ctcccgcgag
    13441 ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta tgaaatccgc
    13501 tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc catcgcccag
    13561 gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg ttttgcgctg
    13621 tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt ggaatttttc
    13681 tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg gatgtggctg
    13741 attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat catgtccgac
    13801 gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg caaaataagt
    13861 aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc aacctgctgt
    13921 gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat taccactggc
    13981 ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg gggtatgtcg
    14041 aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa cctggaacgc
    14101 tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa atgagcctgc
    14161 atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat ctgccagcac
    14221 atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc agcctgagcg
    14281 aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag ttggctgcgt
    14341 ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac tgtctgtcga
    14401 tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag gagcagggat
    14461 gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc
    14521 ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg tggtgttatt
    14581 aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga ttggcgaatt
    14641 actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg agcagagcga
    14701 agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt ttaccggcgg
    14761 aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa acctgatgcg
    14821 cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt ccatctgctg
    14881 gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc gatcacctgt
    14941 caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag cccgcaggtg
    15001 attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc gttgggcgag
    15061 gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat caatacgctt
    15121 ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa aagtgaaata
    15181 caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga aaagttgctg
    15241 gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc aacgcaaaat
    15301 ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa tggcctaccg
    15361 ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca cagcattaat
    15421 ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct ctgtgggccg
    15481 ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc cacggggtta
    15541 cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact ggaaagttat
    15601 gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt cgactctgcg
    15661 cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta aaaagacggt
    15721 aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt cgtagagttt
    15781 caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc ctacaggagt
    15841 gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg cgctgtatca
    15901 ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca tggaaaccat
    15961 ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg gtaagctctt
    16021 cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc agtttggtac
    16081 taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac tggcgatgga
    16141 agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct tcggctggca
    16201 acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg gttcaaatct
    16261 ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt
    16321 tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct ttaacccggt
    16381 cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg ccatgtttat
    16441 tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg cattacgctc
    16501 gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc aactcggaga
    16561 cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga tggaagggga
    16621 gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg aacaggatca
    16681 agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg ccactcactc
    16741 attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc cacgcttgca
    16801 acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc gtgagccgca
    16861 tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt tgctctcccg
    16921 ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga tgcgtggcgc
    16981 gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct gtggttccct
    17041 gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca
    17101 tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg cgattgaagc
    17161 cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca tcttaccgac
    17221 atactccgcg cactccgctt taaccacagg acaactggct ttctcactga tcatgatcgt
    17281 agggctttac accctgttct taatcgccga agtctacctg atgcagaaat atgcccgtct
    17341 ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc
    17401 atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt gatcctggtg
    17461 gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc gctggtggcg
    17521 cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg ggaaggcaat
    17581 caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag agtgtatgca
    17641 gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct gttcttccgt
    17701 ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa aatgtgggat
    17761 gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc cttcggcaac
    17821 ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta tctcggcagc
    17881 ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct tgggatggtg
    17941 attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca tctgcgttca
    18001 cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct ggcaggttac
    18061 tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa cggtccttcc
    18121 aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa tttcgtcgaa
    18181 tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct gacggtgatg
    18241 gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat gcaatttggc
    18301 gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag tgtgagtcca
    18361 atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag cattatgttg
    18421 gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag ctactacaaa
    18481 atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt gtactaagga
    18541 gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt tcgctctcca
    18601 cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag gcctgatgcg
    18661 gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg aaacatatcg
    18721 atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc gcaatctgca
    18781 ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag tcgtcatggt
    18841 gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga cgcatggcca
    18901 acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat cgcctatctc
    18961 ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa gggctgcccg
    19021 cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa aacaggcgaa
    19081 gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca ggcagatacg
    19141 tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact ggataacgcg
    19201 aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt taccgggcat
    19261 cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc aaacttgtgc
    19321 cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc atcggaactc
    19381 aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc aatgctgacg
    19441 gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg aaggatcacc
    19501 gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta tttactacaa
    19561 cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat catggcagcg
    19621 ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac ttcagtgctg
    19681 tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga gctcaactgg
    19741 acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt tgaacgctgg
    19801 cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca gactttacag
    19861 cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt gaaactgacc
    19921 ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg ttttacgcaa
    19981 atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa gagcattcag
    20041 ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt agaccggaaa
    20101 aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt aactataacc
    20161 gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac cattgaggat
    20221 tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc tcacctcttt
    20281 ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc gtcccacgac
    20341 cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat cgtaatggtc
    20401 gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag acgggttagg
    20461 tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct ggatcacttt
    20521 gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac tcacggtaaa
    20581 caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt tttgatcgga
    20641 ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt ctggcgtcgc
    20701 accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca gactggttcg
    20761 tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca ggaaggggat
    20821 atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca gacgggtgcg
    20881 tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt gctcttccag
    20941 ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg cactcaccga
    21001 aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg gtttcactgg
    21061 ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac tggatttcga
    21121 aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc ccgcttctac
    21181 gtcacgactt aaacgcaaca ctttcctgtt gggtggaagg cattgccgat acccgcttat
    21241 tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca cgataagttg
    21301 ggtgatcttt cttatacagc tgggagatct ctgcctcgcg gaaggtcagc tcattgagtt
    21361 gattatcaac gttcacaatc tgctcaagaa cggctttggc ttccaggtta aggtcaaccg
    21421 aatcgcgctg ctggcgataa acgttgagtt tttcttccgc ctggtccagc tcgctgcgca
    21481 cttcaggtaa ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc gcctggcgag
    21541 cgatattctg ttgcaaatag ttgttagcga tgctgttcag aatacgagta atcaactggg
    21601 gatcatcacc agtcatggta agttccagca tcccgctttc tttactgcgt tcgctaacgg
    21661 taaaggtttc ctgcaatgcg ttaatcgctt ccagttcggt acgctggctc aggacaaact
    21721 gtgttcctgg tttggcctta atgtccgcga tagtcagcgc aacgccatct ttttccagac
    21781 gctggccgac cataccattg acggtgaact cttcaccttc cagtgtatag tggccgtttt
    21841 ccccaaccgt gagtgtcagt tgctgatcct gaccattcag ttgtggaata tgcatccagc
    21901 tgatcgccag ctcacctagt ttttctttgg ttaatctcgc ccagccgcga cccacaatcg
    21961 gaaaatactt ctgctcaact atgtcgcgca gattcagttc agcaatggtt ttaccgagaa
    22021 tcatgcgcga ttgcagcagt tggatctccg gtgcagactc gggcgatgag ttagggatca
    22081 tatcgctcag gccgctgaga atggcgttgc cctgtttttg ctcaacctgg accagagtat
    22141 ctgcctgata aattggtgtg cttaacagcg agtaagcgac agcgatcagc gtgaataacg
    22201 cggtcacgct gataataaac ttacggtgat cccataactc gccgaccaga cgaagcagat
    22261 cgatctcatt ttcctgagtg ctgcctggtg gcgtattcat atttttagtt gtcatcgtat
    22321 tttataaact caaattaccg gctgaggcgc ttcgcccatt cctgactggc gcgctccaac
    22381 ataccgtaga catgttcaaa tgcgtcctga cttttacgat agggatccgg gatctctttc
    22441 tgttccagcc attgcccaaa cagcattgtc ttcccgcgga cctcgggagc aattgcggta
    22501 acctgagcaa tatgttccga ctccattgcc agaatcagat cgtaatttcg tgccatctca
    22561 gcagtgagct tacgtccggc atgtccttca agagatacgc catgattagc ggcaacatct
    22621 gccgctgtcg catctgcagg gtgttttacc agaccatgaa cacctgccga tttgactttt
    22681 acgcccggca gacgtttacg cagcaagcgc tcgccaatgg gcgaacggca aatgttgccg
    22741 gtacaaacca ctaggattga gttaaatttt agttgggcca tgtttgaatc caccgtgaag
    22801 tctctgtcag gtcatgcacc ccggaaatcg tcggtaccag cagagaaatc acacggttcc
    22861 aacgagccag cggcgcggta gtgacataaa caatgtcgta aggttcaagc tggaattccg
    22921 ttccgagtat cattgccgat gcgtccttcg cattcagctg ataaatattg gcgattttgc
    22981 cattctgctt gttttgcgtt gcacgaataa caaaaatccc cgtcgcatca gcgacatcct
    23041 gattcatacc ctcagcgtta cccagcgctt ctgccagggt cataccgctg cggtccattt
    23101 tcagcgtgct ctgtttaccc acttcaccca tcacgaagac tttcagatca tcgttgcgcg
    23161 gaataaataa aatatcgccc gggtgcagca gtttgttctg gcgcaagtcg ccacgctgca
    23221 tcagcgcata gaggttcact ttggtcttta cgccattctg ggtgagcacc acgttgcgcc
    23281 agtctgcatc tgcggtcaga ccgcccgcgg cgttaatcgc atccatgatg gtcagaggaa
    23341 tattggtaat cggctgctga cccgatttgc tgacttcgcc ggtgacgtac gctttttgcg
    23401 atcggaacgc cgcgacgctg acatcgactt gcgggctttc aatgactgaa tcgagacgcg
    23461 ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc cactttcagg cgaccaatat
    23521 aggggtagaa aatcgcacca tcggcgttaa cccagttgcc ggtatcactg gcgctacggt
    23581 attgccctgc gggggtggtt agttccgggt gatcccagac cgtaaccatc aacacatcac
    23641 caatcccgat acgatattca tagtttgcga tttgctgctc cagctccggg ttagcctgcg
    23701 acataatcgg tttggcgcgc agctgatcaa tcaatcctgg cgtgacggga tagatattga
    23761 ccattttgtc caggtcatac tgattgtcag gtagttcaat gacatccttg ttactggtcg
    23821 ataaattctg accaggaaca agggtacatg ctgtcaaaga cagaacagcc aatgtcagta
    23881 ccgaaaactt aaaaatgttc tttttcattg gtttatattt atcagtcaat gtaatgttat
    23941 gaattacgtc aggttattgt ccaaccggta cttctcgctc tgccgtcatt ggatcgagct
    24001 ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa tggtgtccac gaaaccaccg
    24061 cgcggttgcg gttaggtcca atggtcatca agtcgaacgg aatcgagata taaaaacctt
    24121 tactaaagcc gccttcgccg tagtcatctt tagatacgtt actgattgcc gcccataccc
    24181 ctaccgccac gccgctgtca aagcgtttgg cgacgtcgat cgttgcgcct ttatcttttg
    24241 ccagatattg cccaacgcta agtttcatca gtacgccatt gagcgtcggc gggttccagt
    24301 aagccgtcac gaaacctgtg ggcgtggaat aatcggtgaa gcgcatcatg ttgtcccagt
    24361 cacgctgctt cacgtagtta acgtccacac ccagcgccca gctggcatct agcgggcgat
    24421 aaagaagctc ggaaccgaca ccggcataca tcgtttccag ataaccgcca tacacctggc
    24481 catagaaacc attgcctaag tcggcaaagt aattcgcttg caagttgttg agataaacgt
    24541 cattgcgaac gtaatcacgg atatgcgtgc gcacgcgggg cagggtagag tccgcgggta
    24601 acagtgaaga cttaaacttg tcgtagttgt tgtaaatatt ggtgaaaata ccgtcatcaa
    24661 gcagcaggtg gtcggtaaac cagtagcggg cgctggacat cagccccagc tggaacatat
    24721 agaaatcttc cgggccgccc agcgactgtg aaagtgttgg gttgaaagag tagctaaagc
    24781 gatcttcacg aatacggtag ccccgaccaa aggcagaaag atcttctgct tcaacacgtt
    24841 gttgttgcag ttgctctgat ttaccaggcg ctgtgccggc cagctgtttg cgcaggctgg
    24901 ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg cttttgcgtc acgctaatct
    24961 tctcaacgcc ttgcggcagg ttattcacca gaatccggtt ggcacgatcg accgcttcgc
    25021 gagagtcacg gtatttgtat tgctgaccag acatatacag cgtcttatcg cgtagctgaa
    25081 tttctggcgc gtcaaagccc gcgttatact tcaggtgaat aagttgtata tttaaaatct
    25141 ctttgactat caatgaatta gtataagaag gtcattatta gtcaaaataa aatcatttgt
    25201 cgatttcaat tttgtcccat tcccgtccac ggctgtccct ataccgcgcc gccattgaat
    25261 ctgatttatg tccgagaaga cgttgagcaa acttatcgcc aatctgatta cggtatagcc
    25321 tcgccgacag gctacgcagt tcatggaatg ttggcgggtc tccatcaaat gagagtcctg
    25381 atgcatttct cgcctttgta aaatacttcg atactgtttt cggggaaagc ggatcgtgat
    25441 gctttgatgc gattatagtt tcactgctgc tggcctccct gcatttctgt agtgtatcag
    25501 ccaatgagat attgagcgcg tcaatcgtta gcgtcagcgg aatggcgagc ttagcccctg
    25561 tt
//
LOCUS       DAJTUN010000052        25321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTUN010000052 DAJTUN010000000
VERSION     DAJTUN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.052"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..103
                     /locus_tag="OO470_001996"
     CDS             <1..103
                     /locus_tag="OO470_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6251247.1"
                     /translation="MPAIIKRPLLCAPGKPMLLGFSDSSYQQFFHEV"
     gene            107..1234
                     /gene="dapE"
                     /locus_tag="OO470_001997"
     CDS             107..1234
                     /gene="dapE"
                     /locus_tag="OO470_001997"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6251248.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1262..1462
                     /locus_tag="OO470_001998"
     CDS             1262..1462
                     /locus_tag="OO470_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6251249.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1572..2270)
                     /gene="ypfH"
                     /locus_tag="OO470_001999"
     CDS             complement(1572..2270)
                     /gene="ypfH"
                     /locus_tag="OO470_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6251250.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2344..4359)
                     /gene="tmcA"
                     /locus_tag="OO470_002000"
     CDS             complement(2344..4359)
                     /gene="tmcA"
                     /locus_tag="OO470_002000"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6251251.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4374..5237)
                     /locus_tag="OO470_002001"
     CDS             complement(4374..5237)
                     /locus_tag="OO470_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6251252.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5405..6118)
                     /gene="purC"
                     /locus_tag="OO470_002002"
     CDS             complement(5405..6118)
                     /gene="purC"
                     /locus_tag="OO470_002002"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6251253.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6331..7365)
                     /gene="bamC"
                     /locus_tag="OO470_002003"
     CDS             complement(6331..7365)
                     /gene="bamC"
                     /locus_tag="OO470_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6251254.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7382..8260)
                     /gene="dapA"
                     /locus_tag="OO470_002004"
     CDS             complement(7382..8260)
                     /gene="dapA"
                     /locus_tag="OO470_002004"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6251255.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8406..8978
                     /locus_tag="OO470_002005"
     CDS             8406..8978
                     /locus_tag="OO470_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6251256.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     TFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            8978..9448
                     /gene="bcp"
                     /locus_tag="OO470_002006"
     CDS             8978..9448
                     /gene="bcp"
                     /locus_tag="OO470_002006"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6251257.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            9701..10318
                     /gene="hyfA"
                     /locus_tag="OO470_002007"
     CDS             9701..10318
                     /gene="hyfA"
                     /locus_tag="OO470_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6251258.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10318..12335
                     /gene="hyfB"
                     /locus_tag="OO470_002008"
                     /pseudo
     CDS             10318..12335
                     /gene="hyfB"
                     /locus_tag="OO470_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12346..13293
                     /gene="hyfC"
                     /locus_tag="OO470_002009"
     CDS             12346..13293
                     /gene="hyfC"
                     /locus_tag="OO470_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6251259.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13310..14749
                     /gene="hyfD"
                     /locus_tag="OO470_002010"
     CDS             13310..14749
                     /gene="hyfD"
                     /locus_tag="OO470_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6251260.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            14761..15411
                     /gene="hyfE"
                     /locus_tag="OO470_002011"
     CDS             14761..15411
                     /gene="hyfE"
                     /locus_tag="OO470_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6251261.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15416..17002
                     /gene="hyfF"
                     /locus_tag="OO470_002012"
     CDS             15416..17002
                     /gene="hyfF"
                     /locus_tag="OO470_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6251262.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            16992..18706
                     /gene="hyfG"
                     /locus_tag="OO470_002013"
                     /pseudo
     CDS             16992..18706
                     /gene="hyfG"
                     /locus_tag="OO470_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            18716..19261
                     /gene="hyfH"
                     /locus_tag="OO470_002014"
     CDS             18716..19261
                     /gene="hyfH"
                     /locus_tag="OO470_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6251263.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19258..20016
                     /gene="hyfI"
                     /locus_tag="OO470_002015"
     CDS             19258..20016
                     /gene="hyfI"
                     /locus_tag="OO470_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6251264.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20009..20422
                     /gene="hyfJ"
                     /locus_tag="OO470_002016"
     CDS             20009..20422
                     /gene="hyfJ"
                     /locus_tag="OO470_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6251265.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20452..22464
                     /gene="hyfR"
                     /locus_tag="OO470_002017"
     CDS             20452..22464
                     /gene="hyfR"
                     /locus_tag="OO470_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6251266.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            22486..23334
                     /locus_tag="OO470_002018"
                     /pseudo
     CDS             22486..23334
                     /locus_tag="OO470_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(23372..24433)
                     /locus_tag="OO470_002019"
     CDS             complement(23372..24433)
                     /locus_tag="OO470_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6251267.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            24646..>25321
                     /gene="bepA"
                     /locus_tag="OO470_002020"
     CDS             24646..>25321
                     /gene="bepA"
                     /locus_tag="OO470_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6251268.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTF"
BASE COUNT         5570 a         6810 c         6684 g         6257 t
ORIGIN      
        1 aatgcctgca attatcaaac gtccattgct ctgcgcgcct ggtaagccta tgctgctggg
       61 tttcagtgat tccagttatc agcaattttt ccatgaggtg tagtctatgt cgtgcccggt
      121 tattgagctg acacaacagc ttattcgccg cccttccctg agtcctgatg acgcaggatg
      181 tcaggctttg ttgattgaac gtttgcaggc gatcggtttt accgttgaac gcatggattt
      241 tgccgatacg cagaattttt gggcatggcg tgggcaaggt gaaacgttag cctttgccgg
      301 gcataccgac gtggtgccgc ctggcgacgc cgatcgttgg atcaatccgc cgtttgaacc
      361 caccattcgt gacggcatgt tattcgggcg cggtgcggca gatatgaaag gctcgctggc
      421 ggcgatggtg gtagcagcag aacgttttgt cgcacaacat cccaaccata cggggcgact
      481 ggcatttctg atcacttctg atgaagaagc cagtgcccac aacggtacgg taaaagtcgt
      541 cgaggcgtta atggcacgta atgagcgtct cgattactgc ctggtcggcg aaccgtcgag
      601 tatcgaagtg gtaggtgatg tggtgaaaaa tggtcgtcgc ggatcgttaa cctgcaacct
      661 caccattcat ggcgttcagg ggcatgttgc ctacccacat ctggctgaca atccggtaca
      721 tcgcgcagca cctttcctta atgaattagt ggctattgag tgggatcagg gcaatgaatt
      781 cttcccggcg accagtatgc agattgccaa tattcaggcg ggaacgggca gtaacaatgt
      841 tattccgggt gaactgtttg tgcagtttaa cttccgcttc agcaccgaac tgactgatga
      901 gatgatcaaa gcgcaggtac ttgccctgct tgaaaaacat caactgcgct atacggtgga
      961 ttggtggctt tccgggcagc catttttgac cgcgcgcggt aaactggtgg atgcggtcgt
     1021 taacgcggtt gagcactata atgaaattaa accgcagcta ctgaccacag gcggaacgtc
     1081 cgacgggcgc tttattgctc gcatgggggc gcaggtggtg gaactcgggc cggtcaatgc
     1141 cactattcat aaaattaatg aatgtgtgaa cgctgccgac ctgcagctac ttgcccgtat
     1201 gtatcaacgt atcatggaac agctcgtcgc gtgatgagtg gttctgcaag aggaaataag
     1261 catggactgg ctggctaaat attggtggat tctggtgatt gtctttttgg taggcgtcct
     1321 gctgaacgtg attaaagatc tcaagcgcgt cgaccataag aaatttctcg ccaacaagcc
     1381 ggagcttccc ccgcatcgtg atttcaacga taagtgggac gatgacgacg actggccgaa
     1441 aaaggatcaa ccgaagaagt aagctgagtg ctcaaccgta ggccggataa ggcgttaacg
     1501 ccgcatccgg caatggtgaa cgatccctga tgcgacgctc acgcgtctta tcaggccagg
     1561 taatctgtct tttacatcat ttcaatcaca tcatcatcgc caggcttacc gccacttagc
     1621 gcttggtcga aataatgttt cggaatggta taacgcaagt gatccagggc aaattgcata
     1681 ctgcgattgt cgatcgcatg gcctaaatcc tccacgatat ccagcgtcac gtcaccaccg
     1741 gcactgatta acgcttcctg cgccgcaact gcatgcgcca gatcgataac cggatcttca
     1801 ccaccatgaa tcaagtgaat tgtcgtggct gtagacgcgg tctccggcag gctcgcatag
     1861 cgtccgttaa aggcaatgac gcgcgaagca aggcccggtt cggctttaat gctctccagc
     1921 gccataatcg cgccttgtga gaaaccaatt agcgccgtgg cgttagcccc tactccgctc
     1981 tgtttctgcc agtagcgtac cgtttcaata aacgtcggca tgatggcatc cacacgcgcc
     2041 tggcgattat cttccgtaat accctgcact gaaaaccact gacgccccgc cggattaccg
     2101 ctcggctccg cgccaccgac actcaccacc agcgcatcag gaaacagcgg tgcaaaccag
     2161 ctgcctattt cccccattgc caccgggtta tccccgacac cgtggaaaag cagtaacaac
     2221 tgttgtgcag gtttatccgg gctttgaaca acaaaatggt catgtttcat ggcgaactcc
     2281 ttaactgatg tcattaattt tacgccgctg actcacaatg accatgggag aaaattgaag
     2341 gagtcagtga aaaaattgcc attgcgttat gcgatcgcgc agacgctcag tgcgaacctc
     2401 atttagtgcg aacagcgcct gcgcggcctc ttcccgctgg cgaaccagta acatcttgcg
     2461 gcctgaaagt ttaagtgtgg tacataactg cgcgtcactg gcgtttttct gtaaacgccc
     2521 acgcagcgcc ggtaatgcca gttcactggt ttgtagcaga cgcaataagc aacctaacga
     2581 tgttaatagc ggacgatgag cgaaagcaaa accggccagt tcaagccagt cgtcgtcaga
     2641 aaggatggca tcgtttagtg gatcaacagg aagcatttcg ccattccact gcgcgagagc
     2701 ttgcgcatcg cgacgtaaac ggtaatgctc acgttcagcc agctgtttac ccgcatcact
     2761 catcggtaac agcgccatcg ccgtatagca accgctgctg gcttcccgat gattacccat
     2821 ccgcaccagc acaaaaccgc agcgttgcca gaaacgccat aactccccag tgtaaccaaa
     2881 actcaccgaa agatagtcga ggtcatgcgt atattgcaaa gctccagcaa taagttgtcg
     2941 ccctgtgcct tcccgctgac gagccggatg aactgctatc cggctaaccc gccttccgcg
     3001 caatgtcgcc gccagtggat tgctgccgtg cgccgccagc gactgggcca ccagattacc
     3061 ccgcgggcga cgaaaacctg cccataccgc ctgactgagt tcttgagata atccgccctc
     3121 atccaccagc cacaacgccc cggcaatctc gttttcgcca gccgcctgta aaaaatgttg
     3181 ccctggtgca tccatcatcc ggcgtaaatc cagcggcgag gtccggtagt gcgcaccaga
     3241 caataactga taaaccttta acggcgtttc tggctcgctt cgccataacg tttgttcaaa
     3301 tgcggaaatg acgatattgc cttgtggtgt atgggtgaag ttttcatcgt taaaaaccag
     3361 tgcctcacta accatttttt ccagcgggca tccctgtgcc cagcggatcg gctgttgcag
     3421 ttcaaaacgg tgtaaatgcg gaaagcgagc gcaaaatttc agcaaaaaac cacgtccggt
     3481 accttcgtag ccctgcaccg tagtggttaa caacgttcga ggaaaacgcg ataccagttg
     3541 atgcaacaac ggcgcaggta tggctgcggc ttcgtcgacc accagccagt cggcttgctc
     3601 atcgctggct aacaaggcat ccggtgcaat aaagcgaaac ttctcgcccg caaattgtgc
     3661 cagtacatcc gttgccgctt ttgcgggcgc ggtgacaatc gcactgcccg caatacgaga
     3721 aatgagttgc cctgccaacg ccgacttacc gcgcccacgc gcagccgtta ccgctgccac
     3781 gcccggcggc atggtcagta gctgctgtaa gagttgctgt tgttctggtt gtggtgcgcc
     3841 ggtcgcgggg tgccagtcag tacggggagt aaaatgcgcc aacgagaacg gctggttttg
     3901 ccgccagagg atagcgtcgt tatccgccgt aagtacgcgt ttgagatgct ggacaaaatg
     3961 cggcgtcgca atagggtcag ggcaatcact ccagcgcagc gagtcggcat caggttggtt
     4021 ttcccactct tcccatacag ggagtaacaa aaccagccag cttcccgctt tcaacgttcc
     4081 gctaagtgct gcaaaggcag cggcatcaaa gccgtggcgg gcgtcgaata ccgcatgccg
     4141 gaactcgcgc ccaagtaaag tttgtagcgc cgagggtgaa cagtggtttt cagcatctag
     4201 ctgcggcgaa atccacagcc agtcgccagg taaggcatca cgcaacttaa gagcatgatc
     4261 aaaacaccaa ccctcttccc cgctcaacac cagcaagcgg cagatccctt cacgtttcat
     4321 ttgcgctgtt aatgtgtaaa gcgcagtcag ttcagccatc cctgccccgg aaattaaatg
     4381 cttttaccaa aagtattgca ttgtgccgga tcgccgctgt cgaaaccacg tttaaaccag
     4441 ctgtagcgtt gctgagaagt gccatgtgtg aaactgtctg gtactactcg cccctgactt
     4501 tgctgttgta aacggtcatc gccgatggcc tgcgccgcat tcagcgcctc ttccagatcg
     4561 ccagtttcca gaacgccttg ctgctgcata ctatgccccc agacaccggc aaaacagtcg
     4621 gcctggagtt ccatacgcac agataagcgg tttacttccg cctgcgtcgc gttttgttgc
     4681 agttgacgaa ctttcggctc gatgcctaac agtttctgca catgatgacc gacttcatgg
     4741 gcgataacgt acccctgggc aaaatcgcca tccgcgccaa gtttgtcttt catgtcatca
     4801 tagaaggaga gatcgatata aactgtgcca tccgccgggc aatagaacgg ccccataatg
     4861 gactggcccg ccccgcagcc ggtgcgcgtc attccacggt acatgaccag cttcggttgc
     4921 tgataggtct tacccatctt ttcgaactgt tgtccccagg tgtcttccgt ggttgccaga
     4981 atcaccgagg tgaattttgc ggcttcgtct tcattagggc taattgaccg cgttgattgt
     5041 tgttgagaaa ccggctgccc ggtcatcaac ccggttaaat caacgccata gtagcctgca
     5101 accagcacga ctatcagtaa aatcagcccg cctttaccgc tcggcagacg gaaaccggga
     5161 ccgcccattg acggaccgcc agagctgttg cgcctgtctt caacattgtc actttcacgt
     5221 cgcccttgcc aacgcataga tacctcaaca atatattcat tatagaaatg atcgtaggtg
     5281 gttcagagga agattaccac aggaaagcgg gatgaaatgg cggaggaatg agcgttcaat
     5341 gcatatattc cgagagcggt tgtcttgccg tgccagctgc acggcaagat gatgaacaga
     5401 aaaatcagtc cagctgtaca cccaggcggc gggcgacggc ttcataggct tcgatcaggc
     5461 caccgaggct ctggcggaaa cggtctttgt ccattttctc cagtgtttct ttgtcccaca
     5521 ggcggctgcc gtccggggag aactcatcgc ccagtaccac ttcacctttg tacagaccaa
     5581 actccagctt gaagtcgacc agaatcagac cagcatcatc gaacagtttt ttcagcacgt
     5641 cgttcgcttt gtaggtcagc tctttcatac gcgccaggtt ctctttgctc acccagccaa
     5701 aggtttcgca gtaagattcg ttgaccatcg gatcgtgcat ggcatcgttt ttcaggaaca
     5761 gatcgaacag cggcgggttc agctcaatac cttcttcgat tccaagacgt ttcaccagag
     5821 agccagcagc acggttacgc acgacacact caaccggcac catatccagc tttttcacca
     5881 gacattcggt atcggagagc agacgctcca tttgagtcgg gatacccgct tcagccagtt
     5941 tgctcataat gaagtagttg aacttgttgt tcaccatacc tttgcgatca aactgctcaa
     6001 tgcgcgcgcc atcccctgct gacgtatcat tgcggaattc gagcaccaac aggtccgggt
     6061 tttccgtgct gtatacggtt ttcgctttac cacgatacaa ctcagcttgc ttttgcatct
     6121 ttatcactcc tgggtgtgaa ttaacgtttt aaaatctttt gctgtctggt gtgccggatg
     6181 ttttgtcgga tgcggcgtga acgccttatc cgaactacgt ccgacaaaca aatttcgtgc
     6241 gaattaccgc taaaatcgcg ttttcctgtc gacgcacacg tttgcgtatc atatcagaaa
     6301 aaagggccgg atgattccag ccctgtattt ttacttgcta aacgcagcct ggaagacagc
     6361 taccagcgcg tcgttctgac tctgagtcag agtatgacct ttcggatcga tgaactgtag
     6421 gctgctgcgg ttatctaaat cgccaacctg cagtttatag tcaccggatg ccaggcctgg
     6481 atcgctcgcg cccagttcct gccagtcgct gtcagacagc ggcttataag ttacggccat
     6541 gttgccctgc gaacgggtgc tgtcggtcac tttcatgccc actttttcca gcgccgctgg
     6601 cagacgttgc caaaccacat tgaacggccc gcgtacgacc agcattggta aaccggtgtc
     6661 atcagctgca ctttgtacgt ccatagtggt ggaggcacga ttttgcgcag cgttcgcggc
     6721 gtcagtggca gatttatcca gaccggcgga aataacgttc atcatctccg tgctgtaacg
     6781 ctgcatggaa gccgcgtctg caaccggttt gcccgcctgt tccaggttca gcagtttaac
     6841 cgtaaccgcc tgctgataac cctgcggctt aacagagatt tgataacgac cacgatactg
     6901 ctcgtcttcg tccagacggt tccattgtac ccaatcggtg gtcagtgtct gaccagcatc
     6961 atcacgttgg gtgatggtgt agtttttcgc ctgcagcacg ctaaccacct gcggccacag
     7021 agtattgcca cgaccatttt ccaccagcaa tgaagcggta tcgcccgtga actgggtacg
     7081 cgcgccagaa accagtgcca acggctgggc tggtggacga atgtccagcg ccttaccgac
     7141 agcaccacta ccgttggtca ccgggattgc ataatcaccg gaggtcaccg gcaaaatcat
     7201 tccagccggg gcatgaagct ccgcaagcgg tgccgcttcc aggtaggctt catcaccact
     7261 gacctgacgc ttatagcgtg agtcagaact acaggcagcg agtaataaaa caagcgaaac
     7321 acccgcaacc tttgccaggc gcgacttttg aacagagtaa gccatcaaat ctccctaaac
     7381 tttacagcaa accggcatgc ttaagcgccg ctctgaccgt ctcacgacca ctgtcggtga
     7441 ttggtgtcat tggcaggcgc agcgtatcgg tcgccacaag acccagttcc ttacatgccc
     7501 atttcaccgg gattggattg ggttcgacaa atagtttgtt gtgtaatggc atcagacgct
     7561 gattaataac gcgtgcctcg gcaaaatgcc cttctgctgc cagtttgcac atctgggcca
     7621 tatcacgcgc tgcgacgtta gccgtaacgg aaataactcc atgaccgccc aattgcatga
     7681 agtccagcgc gctcgcatca tcgccgctca gcagaacaaa atcatctgaa accagctctt
     7741 tgatctggtt tacacgcgtt aagttccctg ttgcctcttt gattccgata atatttttta
     7801 ctttcgccag acggcccacc gtttccggga gcagatcgca gccagtacgg gacggcacat
     7861 tatacagaat ttgcggcagg tcagtatgct cagcgatggc tttgaaatgc tgatacaaac
     7921 cttcttgcga cggacgattg tagtaagggg ttaccgtcag gcagccgacg ataccactgt
     7981 cattgaagcg ctgcgtcagg ctaatggctt ccgcagtagc gttagcgccg gtcccggcaa
     8041 ttaccggaat gcgcccatca gccagatcca gcgtcatcat caccacatca gcatgttcgt
     8101 catgatttaa ggtagcggac tcgccagtgg tgccaacaga aacgatcgcc gaagtaccgc
     8161 tggcgacatg ataatcaatc agttttttca agctagcccg acagacatta cctttttcat
     8221 ccatcggagt aacaatcgcg acaatacttc ccgtgaacat gggccatcct ctgtgcaaac
     8281 aagtgtctca atggtacgtt tggtatggca ttaaaagcaa gcagacagaa ccgttctgat
     8341 tgttgtatgc atgatttttt tatgctttcc ttaagaacaa ctcacccctt aaaggaataa
     8401 ccagtttgac actgtcatcg caacattatc tggtgatcac tgcgttgggt gccgatcgcc
     8461 ctggaattgt gaacaccatc acccgtcatg tcagtagttg cggctgtaat attgaagaca
     8521 gtcgcctggc gatgctggga gaagagttca cgtttatcat gctgctttcc ggttcatgga
     8581 atgccatcac tctgattgaa tcaacgttac cgttgaaagg tgccgaactg gatcttttaa
     8641 tcgtgatgaa gcgcacgacg gcgcgtccgc gtccgccaat gccagcatct gtctgggttc
     8701 aggtcgatgt tgcagactcc ccgcatttaa ttgaacgctt cacagcactt ttcgacgcgc
     8761 atcatatgaa cattgcggag ctggtgtcgc gcacacaacc tgctgaaaat gaacgggctg
     8821 cgcagttgca tattcagata accgcccaca gccccgcatc cgcggacgca gcaaatattg
     8881 agcaaacgtt caaagcccta tgtacagaac tcaatgcaca aggcagtatt aacgtcgtca
     8941 attattccca acatgatgaa caggatggag ttaagtaatg aatccactga aagccggtga
     9001 tatcgcaccg aaatttagct tgccggatca agacggagaa caagttaatt tgaccgactt
     9061 ccagggacag cgtgttctgg tttatttcta cccgaaagcc atgacccccg gctgtaccgt
     9121 acaggcctgc ggcttacgcg ataacatgga tgagttgaaa aaagcgggcg ttgatgtgct
     9181 gggtatcagc accgataaac ccgaaaaact ctcccgtttt gcggaaaaag agctgcttaa
     9241 ctttacgctc ctgtctgatg aggaccacca ggtgtgcgaa caattcggcg tctggggtga
     9301 aaagtccttc atgggcaaaa cctacgatgg tattcatcgc atcagcttcc tgattgacgc
     9361 tgatggcaaa atcgaacatg tctttgacga tttcaaaacc agcaatcacc acgacgttgt
     9421 gctgaactgg ctgaaagaac acgcctgatt actttgctcc attccgtgct ggctgcgctt
     9481 gcggccagca tacctcactt ctcgtgatca agatcacatt ctcgctttcc cctgcgacac
     9541 gggtgtcgaa tccatttttt gctgaacgtt aatgaccatc atttttgtac cgttcagaat
     9601 ccagttaata cataacttat tgaatagatt gagttaatca gaatggcatc ctttatacaa
     9661 tatgaaatgc aatgtttcat atcattttca aggagccgac atgaaccgct ttgtggtggc
     9721 cgaaccactg tggtgtacag gatgtaatac ctgtctcgct gcctgttcgg acgtgcataa
     9781 aacgcaaggt ttacagcaac acccgcgcct ggccctggcg aagacgtcaa caatcactgc
     9841 ccctgtcgtg tgtcatcact gtgaggaagc cccttgcctg caggtctgcc cggtcaatgc
     9901 catctctcag agggatgatg cgatccaact caacgaaagc ctctgtattg gctgcaagct
     9961 ttgcgccgtg gtctgcccat ttggcgcaat cagcgcttca ggaagccgtc cggtgaatgc
    10021 ccatgcgcaa tatgtttttc aggctgaagg ctcactcaaa gacggcgaag aaaacgtgcc
    10081 gccccaacat gcgttgctgc gctgggaacc gggtgtccag accgtcgcgg tgaaatgcga
    10141 cctgtgtgat ttcttgccag aaggtccggc ctgcgttcgc gcttgcccga atcaggcgtt
    10201 acggctgatc accgacgata gcctgcaacg tcagatgaaa gaaaaacagc gccttgccgc
    10261 aagctggttt gccaatggcg gggagtctcc cctttccctc actcaggagc aacactaatg
    10321 gatgccctgc aattattaac ctggtcgctg atcctctatc tgtttgccag tctggcttcg
    10381 ctgtttttac tcggtctgga cagactggct attaagcttt ccggcatcac atcgctggtg
    10441 ggcggcgtga ttggcatcat cagcggaatt acgcaattac atgctggtgt aactttagtc
    10501 gcccattttg ccaccccttt tgactttgcc gatttaaccc tgcgaatgga tagcctctcg
    10561 gcatttatgg tgctagttat ctccttgctg gtggtggttt gttcgctcta ttcattgact
    10621 tatatgcgcg aatacgaggg caaaggcgcg gcggcgatgg gcttctttat gaatcttttc
    10681 atcgcatcga tggttgccct gctggtgatg gacaacgctt tttggttcat cgtgctgttt
    10741 gaaatgatgt cgctgtcttc ctggtttctg gtcattgcca ggcaggataa aacgtcgatc
    10801 aacgctggca tgctctactt ttttatcgcc cacgccggat cggtgctgat tatgatcgcc
    10861 ttcttgctga tggggcgcga aagcggcagc ctcgattttg ccagtttcgc acgctttcac
    10921 tttctccggg gctggcgtcg gcggtgtttc tgctggcctt tttcggtttt ggcgcgaaag
    10981 ccgggatgat gccgttgcac agctggttgc cgcgcgctca ccctgccgca ccatcgcacg
    11041 cttcggcgtt gatgtctggc gtaatggtca aaatcggtat tttcggcatc ctgaaagtag
    11101 cgatggatct gctggcgcaa acgggtttgc ctctgtggtg gggcattctg gtgatggcga
    11161 tcggcgcaat ctccgcgctc ctgggcgtgc tatatgcgct ggcggaacag gatatcaaac
    11221 ggctgctggc ctggagtacc gtcgaaaacg tcggcattat tttgctggca gtcggtgtgg
    11281 cgatggacgg tctgtcactg cacgacccgc tgctcaccgt ggttggactg ctcggcgcac
    11341 tgtttcatct gctcaaccat gcgctgttca aagggctgct atttctcggc gcggacgcga
    11401 ttatttcgcg tttgcatacc cacgacatgg aaaaaatggg ggcactggcg aaacggatgc
    11461 cgtggacagc cgcagcatgc ctgattggtt gcctcgcgat atcagccatt cctccgctga
    11521 atggttttat cagcgaatgg tacacctggc agtcgctgtt ctcactaagt cgtgtggaag
    11581 ccgtagcgct acaacttgcg ggtcctattg ctatggtaat gctggcagtc actggtgggc
    11641 tggcagtaat gtgcttcgtc aaaatgtacg gtattacttt ctgtggtgcg ccgcgcagta
    11701 cacacgctga agaggcacag gaagtgccaa atacgatgat cgtcgccatg ctactgctcg
    11761 cggcactctg cgtattaatt gcgcttagtg ccagttggct ggcaccgaag ataatgcata
    11821 ttgcccatgc gtttaccaat acccctcccg tcactgtcgc cagcggaata gcacttgtac
    11881 ccggcacgtt tcatacacgg gtcactcctt cattactgtt gctgttacta ctggcgatgc
    11941 ctttgctgcc aggcctttac tggctgtggt gtcgttcgcg ccgcgcagcg tttcgtcgca
    12001 caggagatgc ctgggcatgc ggctacggct gggaaaacgc gatggctccg tcaggcaatg
    12061 gcgtgatgca gccgctgcgt gtggtcttct ctgcgttatt tcgtctacga caacagctcg
    12121 accctacgct gaggctaaat aaaggtcttg cgcacgtcac cgccagggct cagagcacag
    12181 aacccttctg ggatgagcgg gtgatccgcc ccatcgtgag cgccacccaa cggctggtca
    12241 aagaaataca gcatctgcaa agcggcgact ttcgtctcta ttgcctgtat gtggtcgccg
    12301 cactggttgt gctgctaatc gctattgccg tctaaggaaa tcaccatgag acaaactctt
    12361 tgcgacggat atctggtcat ttttgcgtta gcacaggccg tgattctgct gatgctaacc
    12421 ccacttttta cgggtatttc ccggcagata cgcgcgcgta tgcactcccg ccgcgggccg
    12481 gggatctggc aggattatcg cgatatccac aaactgttta aacgccagga agttgcgccg
    12541 acatcttcag gtctgatgtt ccgcctgatg ccgtgggtat taatcagcag catgctggtg
    12601 ctggcgatgg ccttaccact atttattacc gtttcccctt ttgcgggcgg cggcgatctg
    12661 atcaccctta tctatcttct tgccctgttt cgttttttct ttgctctttc cgggctggat
    12721 accggaagtc cgtttgcggg agtcggtgcc agtcgcgagt tgacgctcgg cattctggtc
    12781 gaaccaatgc ttattctctc actgctggta ttggcgctga tagcaggttc cacgcatatc
    12841 gagatgatca gcaatacgct ggctatgggc tggaactcgc cgctaaccac cgtactggcg
    12901 ttactggcct gtggttttgc ctgcttcatt gagatgggaa aaattccctt tgatgttgct
    12961 gaagcagaac aggaattaca ggaaggcccg ctgaccgaat attccggtgc cgggctggcg
    13021 ctagcgaaat gggggctggg gctgaaacag gtcgtgatgg cagcactgtt tgtggccctg
    13081 tttctgccct ttgggcgcgc gcaagaactt tctctcgcct gcctgctgac ttcacttgtc
    13141 gttacgctgc tcaaggtttt gctgattttt gtactggcct caatcgcaga aaacacgctg
    13201 gcacgcgggc gttttttact cattcaccat gtgacctggc ttggcttcag ccttgctgcg
    13261 cttgcatggg tcttttggtt aaccggtctg taaggagcac tgacggaata tggaaaatct
    13321 tgctctgacg acgttaatgc tgccttttat cggcgcactg gtcgtttcgt tttcgccaca
    13381 acgtcgggcc gccgaatggg gggttttgtt cgccgcgctg accacgctgt gcatgttgtc
    13441 gctgatctcc gcgttttatc aggccgataa agttgccgtc acgttgacgt tggtcaacgt
    13501 gggggatgtg gcattgtttg gcctggtcat tgatcgcgtg agtacgctga ttctgtttgt
    13561 ggtggtgttt ctcggtttgc tggtcacgat ctactccacg ggttatctga cggataaaaa
    13621 tcgcgaacac ccgcataacg gcacgaatcg ttattacgca tttttgctgg tgtttatcgg
    13681 cgcgatggcg ggactggtag tctcctcgac gctgctcggt cagttgttgt tttttgaaat
    13741 tacgggcggc tgctcctggg cgttgatcag ttattaccag agcgataaag cgcagcgttc
    13801 agcactaaaa gcgttgctta tcacccatat cggttcgttg gggttgtatc ttgccgccgc
    13861 cacgctgttt ttgcagaccg gaacgtttgt gcttagcgcg atgagcgagt tacatggcga
    13921 cgcacgttat ctggtttatg gcggcatcct gtttgccgcg tgggggaaat cggcccagct
    13981 accgatgcaa gcgtggctac cggacgcaat ggaagcgcca acaccgatca gcgcctatct
    14041 ccacgccgca tcgatggtga aagtgggcgt ttacattttt gcccgcgcca ttatcgacgg
    14101 cggcaatatc ccgcatgtga ttggcggcgt tggcatggtc atgacactgg tcaccattct
    14161 ttatggcttt ctgatgtatt tgccacagca ggatatgaag cggttgctgg cctggtcgac
    14221 catcactcaa cttggctgga tgttcttcgg cttgtcgctc tccatcttcg gctcgcggct
    14281 ggcgctggag ggtagcatcg cctacatcgt caaccacgcg ttcgctaaaa gcctgttttt
    14341 ccttgtagca ggtgcgctga gttacagctg cggcacgcgc ttgttgccgc gtctgcgtgg
    14401 cgtattgcac accctgccgt tgccaggcgt gggtttctgc gtggcagcgc tggcaattac
    14461 cggtgtgccg ccgttcaacg gcttcttcag taaattcccg ctgtttgccg ccggttttgc
    14521 gttgtcagtg gagtactgga tcctgctgcc cgccatgatt ctgctgatga ttgaatcggt
    14581 cgccagtttc gcctggttta ttcgctggtt tggtcgcgtc gtgccgggca aaccgagcga
    14641 ggccgtcgcc gatgccgcac cgctgccagg gtcaatgcgc ctggtgttga ttgtactgat
    14701 tgtgatgtcg ctgatttcca gcgtaatcgc cgcgacctgg ttgcagtaag gagatgatga
    14761 atgaccggtt ctatgatcgt aaataatctg gcgggactga tgatgctgac atcgctgttt
    14821 gtgattagcg tcaaaagcta tcgcctgtca tgcggatttt acgcctgcca gtcgctggtg
    14881 ctggtgtcta ttttcgccac tctctcgtgc ctgttcgccg cagagcaact gctgatctgg
    14941 tccgccagcg cctttatcac caaagtgctg ctggtaccgt taatcatgac ttacgctgca
    15001 cgaaatattc cccagaacat cccggaaaaa gcgttattcg gtccggcaat gatggcactg
    15061 ctcgcggcgt taattgtcct gctttgcgca tttgtcgttc agcccgtgaa gctaccgatg
    15121 gctaccgggc tgaaaccggc gctggcggta gcgttaggtc attttctgct tggcctgctg
    15181 tgcattgtca gccaacgcaa tatcctgcgg caaatttttg gttactgcct gatggaaaac
    15241 ggctcccatc tggtgctggc gcttcttgcc tggcgagcac cggaactggt ggaaataggt
    15301 atcgctaccg acgccatctt cgccgtcatt gtgatggtgt tactggcaag aaaaatatgg
    15361 cgtacccacg gcacgctgga cgtgaacaac ttgaccgcgc tgaagggata atgagatgag
    15421 ttattctgtg atgttcgctt tactcctgct cacgccgctg cttttttcgc tgctctgttt
    15481 tgcctgccgg aaacgggggc tctctgcgac ttgtacggtg actgtattac atagcttagg
    15541 gatcacactg ctgctgattc tggcactctg ggtggtccaa actgccgctg atgcaggaga
    15601 aatattcgct gcgggactgt ggcttcatat tgatggtctg ggcggtttgt tcctcgccat
    15661 tcttggtgtg attggctttc tcaccggtgt ttactcgatt ggctacatgc gtcatgaagt
    15721 ggcacacggc gagctttcac ccgttacact gtgcgattac tacggtttct tccatctgtt
    15781 tttgttcacc atgctgctgg ttgttaccag caataacctg attgtgatgt gggcggcgat
    15841 cgaagccacc accttaagct cggcgtttct ggtaggcatt tacggtcagc gttcatcgct
    15901 ggaatcagca tggaagtaca tcattatttg tactgttggt gtcgcttttg gtctgttcgg
    15961 taccgtgctg gtatacgcca acgccgccag cgttatgccg caggcagaaa tggcgatatt
    16021 ctggagcgag gttcttaagc aatcgtcctt gcttgaccca acattaatgc tgttggcctt
    16081 tgtgtttgtg ctaattggct ttggtaccaa aaccgggcta tttcccatgc acgcctggct
    16141 gccggatgct cacagtgaag cgccgagtcc ggtcagcgcc ctgctctccg ccgtattgct
    16201 gaactgcgcg ctgttggtgc tgattcgcta ttacatcatt atttgccagg ccatcggcag
    16261 cgatttcccc aaccggttgt tgctcatctt cggcatgttg tcggttgccg tggcggcatt
    16321 tttcattctg gtacagcggg acattaagcg tctgctggcg tactccagcg tggagaacat
    16381 ggggctggtc gcggtggcgc taggcattgg cgggccgctg ggaatttttg ccgcgctgct
    16441 gcacacctta aaccacagtc tggcaaaaac gctgctgttc tgcggttccg gcaatgtact
    16501 gctcaagtac ggcacgcgcg atctcaacgt cgtctgtggg atgctcaaaa tcatgccatt
    16561 taccgccgtg ctgtttggcg gcggtgcgct ggcgctggcg ctggcaggga tgccgccctt
    16621 caacattttt cttagcgaat ttatgaccgt taccgcagga ctggcacgta atcacctgct
    16681 gattatcgtc ctgctgttat tgctgttaac gctggtgctg gcgggcctgg tacggatggc
    16741 tgcgcgggtg ttaatggcga aaccgccgca ggccgttaac cggggtgatc tcggctggtt
    16801 gaccacctcg ccaatggtga ttctgctggt catgatgctg gcgatgggaa cgcatattcc
    16861 acaacctgtc atcaggatcc tggcgggcgc ttccactata gtcctctcag ggacgcacga
    16921 cctgcctgca caacgtagca cctggcatga ttttttgcct tcaggcaccg catctgtttc
    16981 ggagaaacac agtgaacgtt aattcatcgt caaatcgtgg cgaagcgatt ctcgccgccc
    17041 tgaaaacgca gttccccggc gcggtgctgg atgaagagcg acaaacgcct gaacaggtca
    17101 ccattacggt gaaaatcaat ctgctgcctg acgttgtaca ttatctttat tatcaacatg
    17161 atggctggct tccagtcctg tttggcaacg acgagcggac acttaacggt cattacgcgg
    17221 tttattatgc cctttcaatg gaaggggccg aaaaatgctg gatcgtggtg aaggcactgg
    17281 tcgatgccga cagtcgggag tttccgtcag tcacaccgcg cgtccctgcc gcggtctggg
    17341 gcgagcgaga aattcgcgat atgtacgggc tgattccggt tggcctgccg gatcagcgtc
    17401 gcctggtgtt gcccgatgac tggccggaag atatgcatcc gctgcgcaaa gatgcgatgg
    17461 attatcgact gcgccctgaa ccgacgacag attccgaaac gtatgcgttt atcaatgagg
    17521 gcaacagcga tgcgcgggtg atccctgtcg gcccgctgca tatcacctcc tatgaaccgg
    17581 gtcacttccg cttgtttgtg gatggcgagc aaattgtcga tgctgattac cgcctgtttt
    17641 atgtccatcg cggcatggag aaactggcag aaacgcggat gggctacaac gaagtgacct
    17701 tcttatcgga ccgcgtgtgt gggatttgcg gttttgccca cagtgtggcc tataccaact
    17761 cggttaaaaa tgcactgggg attgaggtgc cgcaacgagc acatactatt cgctcgattc
    17821 tgctggaagt cgagcggcta cacagtcatt tgctcaacct tggcctctcc tgccattttg
    17881 ttggttttga taccggcttt atgcaatttt tccgcgtgcg ggaaaagtcg atgacgatgg
    17941 cggaattgct gaccgggtcg cgtaaaacct acggtctgaa tctgattggt ggtgttcgcc
    18001 gcgatattct caaagagcaa cgtctgcaaa cgctgaaact ggtgcgcgag atgcgcgccg
    18061 acgtgtcgga gctggtagag atgctgcttg ctacgccgaa tatggaacaa cgcactcagg
    18121 gcattggcat tctcgaccga caaatcgccc gtgattatag ccctgtaggg ccgctgatcc
    18181 gcgcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact acggcaatat
    18241 tccaaaaaca ctgtttacct ttaccggcgg cgatgttttc tcccgcgtga tggtccgtgt
    18301 caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca tgccggatac
    18361 cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg gctttgttga
    18421 agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa aattgttccg
    18481 ctggcgctgt cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg
    18541 caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct actcctgtac
    18601 cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc tgtataaaga
    18661 gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac agaagatgct
    18721 gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc ccttcgcgcc
    18781 actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc aatgtattgc
    18841 ctgcggtgcc tgcgcctgtg cttgtccggc aaatgcgctg actatccaga ccgacgacca
    18901 gcaaaattcg cgcacctggc agctctatct ggggcgttgt atttactgcg gacgttgtga
    18961 agaagtgtgc ccgaccagag ccatccagct taccaataac tttgaactga ccgtcaccca
    19021 taaagccgat ctctataccc acgcgacgtt ccatctacaa cgttgcagcc gttgcgaacg
    19081 cccgtttgcc ccgcaaaaaa ccgtcgcact ggctgctgaa ttgttggcac agcaacaaaa
    19141 tgctccacaa aaccgcgaaa tgttatgggc gcaagcgagc gtctgcccgg agtgcaaaca
    19201 acgcgcgacg ctgatcaacg acgatacaga tgtaccgctg gtggctaagg agcagctatg
    19261 agtccagtgc ttacgcaata tgtcagccag cccatcacgc tggacgagca aacgcaaaag
    19321 atgaagcggc atttgctaca ggatatccgt cgctcggctt acgtttatcg catcgattgc
    19381 ggcggctgca acgcctgtga aatcgaaatt tttgctgcca tcacccccgt attcgacgca
    19441 gaacgttttg gcattaaggt tgtttcatca ccgcgtcacg ccgatatttt gttatttact
    19501 ggcacagtca cccgggcgat gcgtatgcct gcacttcggg cgtatgagtc tgcccccgat
    19561 cataaaatct gtgtttccta cggcgcgtgc ggtgtcggcg gcggtatttt ccacgatctc
    19621 tacagcgtct ggggcggcag cgacaccatt gtccccattg atgtttggat ccccggctgc
    19681 ccgccaacac cggccgccac cattcacggt ttcgccgtgg cgctcggttt gctgcaacag
    19741 aagattcacg cggtggatta tcgcgatccc accggggtga ctatgcaacc gttgtggccg
    19801 cagatcccgc catcacagcg tatcgccatt gagcgagaag cgcggcggct ggcgggctat
    19861 cgtcaggggc gagaaatttg cgatcggctc ctgcgccatt taagcgacga tcctacagga
    19921 aatcgggtta acacctggtt gcgcgatgcc gacgatccac gtctcaatag tatcgttcag
    19981 caactctttc gcgtactccg ggggttacat gactgaagag tgcggggaaa ttgttttctg
    20041 gacgctgcga aaaaagtttg tcgccagtag cgacgagatg ccggaacaca gctctcaggt
    20101 aatgtattac tcgctggcta tcggtcatca cgttggcgtg attgattgtc tgaatgtcgc
    20161 cttccgctgc ccactgacgg aatacgaaga ttggcttgca ctggtcgaag aggagcaagc
    20221 ccgacgtaag atgctggggg tgatgacttt tggtgagatt gttattgacg ccagccacac
    20281 cgccctgttg acccgggcat tcgcgccact ggcagatgac gcgacgtctg tgtggcaagc
    20341 gcgtagcatt caattcattc atctgttgga tgaaattgtg ctggaaccgg ctatctatct
    20401 gatggccaga aaaattgcgt gagaaggatt tctcattaat aaggactgtt gatggctatg
    20461 tcagacgaag cgatgtttgc cccgccgcaa ggaataacaa ttgaagcggt aaacggaatg
    20521 ctcgcggagc ggttagcgca gaaacacggc aaggcgtctt tattacgcgc cttcatcccg
    20581 ctgccgccgc cgttcagccc ggtacaactt attgaactgc atgttctcaa aagcaacttc
    20641 tattaccgct accatgatga tggcagcgat gtgacggcaa caacagagta tcagggcgag
    20701 atggtcgatt attcgcgtta cgccgtcctt ctcggcagta gtggaatggc ggagctacgc
    20761 tttattcgta cccacggcag ccgttttact ccccaggatt gcacactgtt taactggcag
    20821 gcacgcatta tcaccccggt tctgcaatca tggctcaatg atgaagcaca gcaggtggcg
    20881 ctgcgtttgc tggagaaaga tcgcgatcat catcgggtac tggtggatat cactaatgca
    20941 gtgctgtcac atcttgatct cgacgatctg atcgctgacg tcgctcgtga gatccatcat
    21001 tttttcggtc tgtcttcagt cagtatggta ctgggcgatc atcgaaagaa cgagaagttc
    21061 agcctgtggt gcagcgatct ttctgcctca cattgtgcgt gtctgccaca caatatgcct
    21121 ggcgacagtg tattgctgac acaaacgcta caaacccgac aaccgacctt gacgcaccgt
    21181 gcagatgatc tgtttctctg gcaacgcgac ccgttattac tcttacttgc atctaacggc
    21241 tgcgaatctg cgctccttat accgcttacc tttggcaacc atacaccggg tgcattgttg
    21301 ctggcgcata cctcttccac tctctttagt gaggaaaact gccagctact acaacacata
    21361 gccgatcgca tcgctattgc cgttggcaat gccgatgcct gccgtagaat gaccgatttg
    21421 caggaaagtt tgcagcaaga aaaccaccag cttagcgagc agctcctttc gaatctgggc
    21481 gtcggtgaca ttatctatca aagccaggca atggaagacc tgctccagca ggtagatatt
    21541 gtggcgaaga gcgacagtac ggtgttgatt tgcggtgaaa ccggaaccgg caaagaggtg
    21601 atcgccagag cgatccatca acttagcccg cgacacgaca agccgctggt caaaatcaac
    21661 tgcgctgcca tccccgccag tcttctggaa agtgagttat tcggtcatga caaaggggcg
    21721 tttactggtg cgattaatac ccatcgtggt cgttttgaaa ttgccgatgg cggcacgttg
    21781 tttctcgatg aaattggcga tctgccgtta gaacttcagc ctaaactgct gcgcgtattg
    21841 caggaacggg agattgagcg tctcggcggg agtagaacga tcccggtaaa tgtcagagtc
    21901 attgccgcca ccaaccgtga tttgtggcaa atggttgaag atcgccagtt tcgcagcgat
    21961 ctcttttatc gcctgaatgt cttcccactg gaattgcctc cgctgcgcga ccgtccggaa
    22021 gatatccctc ttttagcaaa gcatttcacg caaaaaatgg cgcgccatat gaatcgttca
    22081 attgacgcca tcccgaccga ggcgctacgc cagttgatgt cgtgggactg gccgggcaac
    22141 gtgcgcgagc tggaaaacgt gattgagcga gcggtactgc tgactcgcgg taacagtctg
    22201 aatttacatc ttaatgtccg acaaagccgt ttactgccga cgctaaatga agattcagcg
    22261 cttcgcagtt caatggcgca gttgctgcac ccgacgacgc cagagaatga cgaagaagaa
    22321 cgtcagcgca ttgttcaggt attgcgagaa accaatggca ttgttgccgg gccccgtggc
    22381 gcggcgacac gattagggat gaagcgcacc acgctgctgt cacgaatgca gcgtctggga
    22441 atctcggttc gcgaggtgtt gtaatctgct tttgcaggag tatgcatgag aaacaaactc
    22501 tctttcgact tgcagttgag cgccagaaaa gcggcaatcg ctgaacggat tgccgcccat
    22561 aaaattgccc gcagtaaagt gtcggtcttt ttaatggcga tgtccgctgg cgtgtttatg
    22621 gcgatcggat ttacttttta cctttccgtt atcgccgatg ccccgtcttc ataggcatta
    22681 acccatctgg tgggcggcct ttgctttaca cttggcttta ttttgctggc ggtttgcggc
    22741 accagcctgt tcacctcgtc ggtaatgacg gtaatggcaa aaagtcgggg cgttattagt
    22801 tggcgaactt ggctgattaa cgcacttctg gtggcctgcg gtaatctggc aggtattgcc
    22861 tgtttcagtt tgttaatctg gttttccggg ctggtgatga gtgaaaacgc gatgtgggga
    22921 gtcgcgattt tacactgcgc cgagggcaaa atgcatcata catttactga atctgtcagc
    22981 ctcggcatta tgtgcaatct gatggtttgc ctggcgctgt ggatgagtta ttgcggacgt
    23041 tcgttatgcg acaaaatcgt cgccatgatt ttgtccatca ccctgtttgt cgccagtggc
    23101 tttgagcact gtatcgccaa tttgtttgtg attccgttcg ccattgccat tcgccatttc
    23161 gcccctacct ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg
    23221 gtcagccatt ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt
    23281 gcagtgctgg tgagtatttg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc
    23341 agcccggcga aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc
    23401 aggcgtggat cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga
    23461 atccccacaa accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa
    23521 ccgcttcgga gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat
    23581 acaccgcgaa gcagctccag aattcagtac ctgcgccaaa ctggaatagc gccacgccaa
    23641 ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca
    23701 gcactgccag cagcagcgaa tagttcagcc cgaagagcaa gaaccccagc caggtggcga
    23761 tccccaccac gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca
    23821 tctccttcca cacctgtcct gccagcccac ggttacgcgg cagcacccgg cgaacggcgt
    23881 tcagcatctg ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat
    23941 agacggctat ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc
    24001 ccatggtcag catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca
    24061 ttaacaccgg atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc
    24121 ccggcatatc gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg
    24181 ccatcagtag caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg
    24241 agcagccaat agattgcagg cgcacggttg gccattccag caaataagcc agcacaatag
    24301 ccaccagcaa cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta
    24361 aaataaccag caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca
    24421 acatttcgag cataaagacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt
    24481 atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac taaaaagtca
    24541 tttgagtggg ctaatcttcg ccgttacact caaaggcggc gaggtgggaa cgatatttca
    24601 cagtatcggt caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg
    24661 aaaaaaaacc tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg
    24721 tttgccgaca gcgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc
    24781 attggtcagg aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg
    24841 ttaattaatg acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg
    24901 catgccaatt cggttaagac accgtttcat ttttttctga tcaacaacga cgaaattaac
    24961 gcctttgctt tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat
    25021 aacgaaagtc aactggcttc agttatggcg cacgaaatct cccacgtcac ccaacgtcac
    25081 ctggcgcgag cgatggaaga tcaacaacgc agcgcgccgc tgacctgggt cggcgcgtta
    25141 ggttctattt tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca
    25201 ctggcgggaa cgcgccaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac
    25261 cgcattggta ttcaggtgct gcaacgctcg ggattcgatc cacaggcgat gccgaccttc
    25321 c
//
LOCUS       DAJTUN010000053        25043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTUN010000053 DAJTUN010000000
VERSION     DAJTUN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.053"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            194..2422
                     /gene="crfC"
                     /locus_tag="OO470_002021"
     CDS             194..2422
                     /gene="crfC"
                     /locus_tag="OO470_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6251269.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2419..3297
                     /locus_tag="OO470_002022"
     CDS             2419..3297
                     /locus_tag="OO470_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6251270.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            3561..5063
                     /gene="proP"
                     /locus_tag="OO470_002023"
     CDS             3561..5063
                     /gene="proP"
                     /locus_tag="OO470_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6251271.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5175..5264
                     /gene="pmrR"
                     /locus_tag="OO470_002024"
     CDS             5175..5264
                     /gene="pmrR"
                     /locus_tag="OO470_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6251272.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5240..6340)
                     /gene="pmrB"
                     /locus_tag="OO470_002025"
     CDS             complement(5240..6340)
                     /gene="pmrB"
                     /locus_tag="OO470_002025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6251273.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6341..7009)
                     /gene="basR"
                     /locus_tag="OO470_002026"
     CDS             complement(6341..7009)
                     /gene="basR"
                     /locus_tag="OO470_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6251274.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(7006..8649)
                     /gene="eptA"
                     /locus_tag="OO470_002027"
     CDS             complement(7006..8649)
                     /gene="eptA"
                     /locus_tag="OO470_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6251275.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8753..10090)
                     /gene="adiC"
                     /locus_tag="OO470_002028"
     CDS             complement(8753..10090)
                     /gene="adiC"
                     /locus_tag="OO470_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6251276.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10227..10988)
                     /gene="adiY"
                     /locus_tag="OO470_002029"
     CDS             complement(10227..10988)
                     /gene="adiY"
                     /locus_tag="OO470_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6251277.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11313..13580)
                     /gene="adiA"
                     /locus_tag="OO470_002030"
     CDS             complement(11313..13580)
                     /gene="adiA"
                     /locus_tag="OO470_002030"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6251278.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13779..14687)
                     /gene="melR"
                     /locus_tag="OO470_002031"
     CDS             complement(13779..14687)
                     /gene="melR"
                     /locus_tag="OO470_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6251279.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14970..16313
                     /gene="melA"
                     /locus_tag="OO470_002032"
     CDS             14970..16313
                     /gene="melA"
                     /locus_tag="OO470_002032"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6251280.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            16428..17849
                     /gene="melB"
                     /locus_tag="OO470_002033"
     CDS             16428..17849
                     /gene="melB"
                     /locus_tag="OO470_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6251281.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(17988..18617)
                     /locus_tag="OO470_002034"
     CDS             complement(17988..18617)
                     /locus_tag="OO470_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6251282.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(18740..20386)
                     /gene="fumB"
                     /locus_tag="OO470_002035"
     CDS             complement(18740..20386)
                     /gene="fumB"
                     /locus_tag="OO470_002035"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6251283.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(20464..21804)
                     /gene="dcuB"
                     /locus_tag="OO470_002036"
     CDS             complement(20464..21804)
                     /gene="dcuB"
                     /locus_tag="OO470_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6251284.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22375..23094)
                     /gene="dcuR"
                     /locus_tag="OO470_002037"
     CDS             complement(22375..23094)
                     /gene="dcuR"
                     /locus_tag="OO470_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6251285.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(23091..24722)
                     /gene="dcuS"
                     /locus_tag="OO470_002038"
     CDS             complement(23091..24722)
                     /gene="dcuS"
                     /locus_tag="OO470_002038"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6251286.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            24903..>25043
                     /gene="yjdI"
                     /locus_tag="OO470_002039"
     CDS             24903..>25043
                     /gene="yjdI"
                     /locus_tag="OO470_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6251287.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         6039 a         6080 c         6487 g         6437 t
ORIGIN      
        1 ctttttttta ttcacatgat atttttatct ttctgacgtt ttaaacgcat cagaagacgt
       61 tatttattct gataaaaaac aacacactaa atataaaatt atttcttgca gtaattataa
      121 aagccgaaaa cagaactcaa aaacaatctg gctaaataaa agtaacaaaa tttgctttaa
      181 ggaagaattt tctatgtaca cacagaccct gtatgagtta agtcaggagg ctgaacgcct
      241 gttacagctt tctcgccaac agttgcagtt actggaaaaa atgcctctct ctgtacccgg
      301 agacgacgcg ccacaactgg ctttaccctg gagtcagcct aatatcgccg aacgtcacgc
      361 gatgctgaat aatgagttgt gtaaaatttc ccgactggaa atggtgctgg caattgtcgg
      421 taccatgaaa gcagggaaat caaccaccat taatgccatt gttggtacgg aggttctgcc
      481 taatcgtaat cgcccaatga ctgcgctgcc gacgcttatt cgccatacgc ccgggcaaaa
      541 ggaaccggta ctgcattttt cacatgtcgc gccaatcgat tgtttaattc aacaattaca
      601 acagcgcctg cgtgattgcg atattaagca tctgaccgat gtgctggaaa tagataaaga
      661 tatgcgtgcg cttatgcagc ggatcgaaaa tggcgtcgct ttcgaaaaat attatctggg
      721 tgcccagcct atttttcatt gtctgaaaag tttgaatgat ttagtgcgac tggcgaaggc
      781 gctggacgtc gattttcctt tttctgctta cgccgccatt gagcatattc ccgtgattga
      841 agtggagttt gtccatctgg cggggctgga aagttatccc ggtcagttga cgttactgga
      901 tacccccggg ccaaatgaag ccgggcaacc gcatctgcaa aaaatgctta accagcagct
      961 ggcacgcgcc tcggcggtac tggcggtgct ggattatacg caactgaaat cgatctccga
     1021 tgaagaggtc cgtgaggcga ttttggcggt ggggcaatcg gtgccgctgt atgtgctggt
     1081 caataagttc gatcaacagg atcgtaacag tgacgacgcc gaccaggtgc gggcactgat
     1141 ttccgggacg ctgatgaaag gctgtattac gccacagcag atattcccgg tgtcgtcgat
     1201 gtggggctac ctggcgaatc gggcgcgcta tgagttagcc aacaacggta agttaccagc
     1261 gccagagcaa caacgctggg tggaagattt tgcccatgcc gcgctcggca ggcgctggcg
     1321 tcatgccgat ctggcggacc tcgaacatat tcgtcatgct gccgatcagt tgtgggaaga
     1381 ttcgctgttc gcccagccaa ttcaggcgtt gcttcatgcc gcttacgcta acgcctcatt
     1441 gtatgctctg cgatctgctg cgcataaact gttgaattac gcgcagcagg cgcgggaata
     1501 cctggatttt cgtgcgcacg ggttaaacgt cgcttgtgaa caattgcggc aaaatatcca
     1561 tcaggtcgaa gaaagtttgc agctattgca actcaatcag gcgcaggtga gcggcgagat
     1621 taaacatgaa atcgagctgg ccctgacctc cgccaaccac tttctgcgtc aacagcaaga
     1681 tgcggtgaat gcgcagttag ccgccttgtt tcaggatgat tcggagccgt taagcgagat
     1741 tcgtacccgc tgtgagacac tgttacagac ggcgcagaac accatcagtc gcgactttac
     1801 gctgcgtttt gccgagatgg aatccaccct ttgccgggtg ttaaccgatg ttattcgccc
     1861 cattgagcaa caagtcaaaa tggaattgag cgagtcaggg tttcgtcctg ggtttcattt
     1921 tcctgttttt cacggcgtag ttccccactt caacactcgc cagctgttca gtgaagtcat
     1981 ttcgcgccag gaagcaacgg acgagcagag cacgcgttta ggcgttgtgc gtgagacttt
     2041 ttcgcgctgg ttgaatcagc ccgactgggg acggggaaat gagaaatccc cgacagaaac
     2101 ggttgattac agtgtgttgc aacgagcatt aagcgcagaa gtcgatctct attgccaaca
     2161 aatggctaaa gttctggcag agcaggtcga tgaatctgtt acggcaggca tgaatacttt
     2221 tttcgctgag ttcgcttcat gtttgacaga attacagacg cgtttacgcg aaagtctggc
     2281 tctgcgtcaa caaaatgaat cggtggtcag gctgatgcag cagcaattgc agcagactgt
     2341 gatgactcac agctggattt acaccgacgc tcagctgtta cgcgatgata ttcaaacact
     2401 tttcacggca gaacgatatt gaccaagacg ttactggacg gccccggtcg cgtgctggag
     2461 tcggttcatc cgcgcttttt agtggatctg gcgcagggcg atgatgcccg ccatcctcaa
     2521 gcccatcagc agcagtttcg tgaacgacta atgcaggaac ttctttcgcg tgtgcagctt
     2581 cagacatgga cgaacggcgg catgttaaat gcgccgctta gcctgcgact gacaatggtg
     2641 gaaaaactgg cgtcgatgct ggatcccggt catctggcgc tgacgcagat cgcgcagcat
     2701 ctggcgctgc tgcaaaaaat ggatcaccgc cagcactctg ctttcccgga gctcccccag
     2761 caaattgccg ccttgtatga gtggttttca gcccgttgtc gctggaagga aaaggcgtta
     2821 acgcaacgag gcctactggt gcaggcaggt gatcagagcg agcaaatttt tacccgctgg
     2881 cgtgctgggg cgtataacgc ctggtcgttg cctgggcgct gttttatcgt tctggaggag
     2941 ttgcgctggg gtgcatttgg cgatgcctgc cgtctgggaa gcccgcaagc ggtggtgttg
     3001 ttgctgggtg atttgcgcga gaaagcgaca caacatctgg cagagagtat caatgcggca
     3061 ccgactacgc gtcactatta ccatcagtgg tttgcctctt cgaccgttcc gacgggcggg
     3121 gatcatgctg attttttaag ttggctggga aagtggacca cggcagataa acaacccgtt
     3181 tgctggtcag tgacccaacg ctggcaaact gtcgcgctgg ggatgccacg actctgttca
     3241 gcgcagcgtt tggtgggggc aatggtcgag gaaatcttct ctgtaaattt ggcgtaaata
     3301 atcagttaca tcaatgagtc ctaaacgaaa tccatgtgtg aagttgatca caaatttaaa
     3361 cactggtagg gtaaaaaggt cattaactgc ccaattcagg cgtcaactgg tttgattgta
     3421 cattccttaa ccggagggtg taagcaaacc cgctacgctt gttacagaga ttgcatcctg
     3481 caattcccgc tccccttttg cggccgtcgc gctgattttt ctggcgtttg cggaaatggg
     3541 ccaactctgc gaggaaagct atgctgaaaa ggaaaaaagt aaaaccgatt acccttcgtg
     3601 atgtcaccat tattgatgac ggtaaactgc gtaaagccat taccgcagca tcactgggta
     3661 atgcaatgga atggtttgat tttggtgttt atggttttgt tgcttacgca ttaggtaaag
     3721 tttttttccc gggggctgac cccagcgtgc agatggttgc tgcacttgcc actttctccg
     3781 ttccctttct gattcgaccg cttggcggac tcttctttgg tatgttgggc gataaatatg
     3841 gtcgccagaa gatcctcgct atcactattg tgattatgtc gatcagtacg ttctgtattg
     3901 gcttaatacc gtcctacgac acgattggta tttgggcacc gattctgctg ttgatctgta
     3961 agatggcaca aggtttctcg gtcggtggtg aatataccgg ggcgtcgata tttgttgcgg
     4021 aatactcccc tgaccgtaaa cgtggcttta tgggcagctg gctggacttc ggttctattg
     4081 ccgggtttgt gctgggtgcg ggcgtggtgg tgttaatttc gaccattgtc ggcgaagcga
     4141 acttcctcga ctggggctgg cgtattccgt tctttattgc tctgccgtta gggattatcg
     4201 ggctttacct gcgccatgcg ctggaagaaa ctccggcgtt ccagcagcat gttgataaac
     4261 tggaacaggg cgaccgcgaa ggtttgcagg atggcccgaa agtctcgttt aaagagattg
     4321 ccactaaata ctggcgcagc ctgttgacat gtattggtct ggtaattgcc accaacgtga
     4381 cttactacat gttgctgacc tatatgccga gttatttgtc gcataacctg cattactccg
     4441 aagaccacgg ggtgctgatt attatcgcca ttatgatcgg tatgctgttt gtccagccgg
     4501 tgatgggctt gctgagtgac cgttttggcc gtcgtccgtt tgtgctactt ggtagtgttg
     4561 cactgtttgt gttggcgatc ccggcgttta ttctgattaa cagtaacgtc atcggcctga
     4621 tttttgccgg gttactgatg ctggcggtga tccttaactg ctttatgggc gttatggctt
     4681 ctaccttgcc agcgatgttc ccgacgcata tccgttacag cgcgctggcg gcggcattta
     4741 atatttcggt gctggttgcc ggtctgacgc caacactggc ggcctggctg gtcgaaagct
     4801 cgcagaatct gatgatgcct gcctattacc tgatggtagt ggcggtgatt ggtttaatca
     4861 ccggcgtaac catgaaagag acggcaaatc gtccgttgaa aggtgcgaca ccggcggcgt
     4921 cagatataca ggaagcgaag gaaattctcg tcgagcatta cgataatatc gagcagaaaa
     4981 tcgataatat tgaccacgag attgccgatt tgcaggcgaa acgtacccgc ctggtgcagc
     5041 aacatccgcg aattgatgaa taagctgaaa cggatggcct gatgtgacgc tgtcttatca
     5101 ggccaattga actcttaagg ttcacttaat ctctgacgcg catactctcc tccaggttaa
     5161 cggaggagag tgcaatgaaa aaccgtgttt atgaaagttt aactaccgtg ttcagcgtgc
     5221 tggtggtcag cagctttctt tatatctggt ttgccacgtt ctgatctttc ttcagccgta
     5281 cccaggcccg cgtgccggaa gtctcttgcc ggttttgcag gaaaaactgc ccgtgatgca
     5341 actgtgtaat gcggctgaca atacttaacc ccagaccaat cccgccataa cggctgtcca
     5401 tacgtacaaa cgctttactc aactccccgc atttactctc atcaatacct ggtccttcat
     5461 cttcaactgc catgaccgct ccgccatctt cttgcagctt aatcataatg ttgctgcctt
     5521 gcgggctgta acgatgggcg ttttctacca ggtttcgcaa taacatccgc agcagggttg
     5581 catcaccctg aacggtgatg tcggcggcgc tctctggcaa tagcagggtt tgctgtcgct
     5641 ggtcgagcat ggtactgagt tcgtcatacg aggggagaat gacatcttcc agcagtttta
     5701 catgttgata attaccggaa gaaaatgact gtccggcacg cgccagttgc agcagctggg
     5761 agacgctctc catcatctga tcaagccgtg ccactaacgg tgctacatca atgtgatgcg
     5821 ttttcgccag cagttccaga tgcaaacgca cccccgccag tggcgttcgc agttcgtgcg
     5881 cgacgtcagc ggtaaacaac ctttcgttat ccagcgtgct ggtcaggcga ctgaccagat
     5941 cgttcaacgc cgaaaccacc gcttcgattt cgagggtggc gctgtgaatg gcaatgggcg
     6001 ttaagttgtc ggcggtgcgc gcttccagct ctttttgcag ctccgccagc gggcgggtga
     6061 tgcggcgtac cgcctgatag cagataaata gcgtcaggct gaccataaag acgccgggga
     6121 caatcaggct ggcgaccgcc tcgcggatct cacgcatgat gtggcgatcg ttgttgcgat
     6181 tgtcgcgtag cgcctgctca aacagctgaa tctgctcggt actttcatgc catagccaga
     6241 agacgctgat cagctcaaac accaacaaaa tggccccgat ggtcaatatc agccgttggc
     6301 gcagcgatat tggtcggcgc agaaaacgca tcagattcaa ttagttttcc tcattcgcga
     6361 ccagcatata gccaaagccg cgcacggtgc ggatacgggc tttgcccact ttgtcgcgca
     6421 gattgtggat atgcacttcc agggtgttgg tcgagggttc attgtcccag ttatagatgt
     6481 cgttgtagag aatttcccga tgtaccggac tgcctgcttt gagcattaac cgtgacagca
     6541 gagcatattc tttgggcgtc agaatcaact cttcaccgcc catccatacc tgacggcgac
     6601 ccatattcag cgtcagattg ccaacaatca gctcactttc gccctgatta ttatggcgtc
     6661 gtagcagggc gcggatacgg gcgtgtaact cttccagcgc aaaaggcttc accagatagt
     6721 catcggcacc gacatccagc ccggcgattt tgtcggtcag cgtatcgcga gcggtgagga
     6781 tcagtaccgg cagggtgtat tttttctgcc ggatacgggc gagaaaatgc agtccatctt
     6841 cgtcgggtaa ccctaaatcc agtaccacca ggctgtaatg accgtcctca aggctttgtt
     6901 ccgccatccg cgcggttgtc acgccatcgc acgcgtagcc ttcggtttgc gccgccagaa
     6961 tcagtccctg caataacagc gtatcgtctt caacaatcag aattttcatt tactcactct
     7021 cctgcaagtt tgcagaatat catccgcggc ctggtaatac ttcgtctcaa cgccagttaa
     7081 tcccaatagc gtggagaata aattgtcttg tgaatagtgt tgcgtttgcg cctgtttttg
     7141 caggcagttc tggtcaacct gataccgctt ttgataatcc tccgacagcc acagcagcat
     7201 tggcacctgt ttttggctat ccggggcgat ggcataaggc agaccgtgca gatagatgcc
     7261 attttcacct aacgattcac cgtggtcaga aagataaacc aggctggtgg taaatttatc
     7321 ctgatgttct ttaagcagat taatcgcttt atcaacaata tagtcgacgt aaaccagcgt
     7381 gttgtcgtaa gtgttcacca gttgctcttt ggtacaggtc tggatctcat tggtgtcgca
     7441 ggttggggta aatttcctga actgcggcgg atagcggttg taataggtcg gaccgtggct
     7501 gccgatggtg tgtaagacaa tcacgccatc accctgcagg ttattgatgt actcttccag
     7561 cccgtggaac agcacttcgt catagcattc gccgttgatg cactgatcag gcagattcag
     7621 cgcggtgacg ttctggtgag gcacgcggtc gcaggcacct ttacagccgc catcgttgtc
     7681 attccacagc acgttgatgc ccgctcgctg aatgatatcc agcacgcctt cctggtgctg
     7741 tgccagctct tctttgtagt gctcacgcgg catatccgag aacatgcacg gtactgaaac
     7801 tgccgttgcc gtgccgcaag atgcggtatt agggaaatag accacgttat ctttcgccag
     7861 ccgcgggtta gttgcacgcg ggtagccgtt gagggagaag ttctccgccc gcgaggtttc
     7921 gccgacaatc aggatggtca aatttttacg tttttcgttc tgcattaacg ggttgcggtg
     7981 cgcgtcttca ccaattcgca ccagcggcag atttgccagt cgctgatggg agtaccatga
     8041 ccagctggca acaatgctgt tagaggggct taaggatttc accagctctt tgttattgcg
     8101 gaacaacgag gcgtagtctt tataaaacag tgcggcgacc agcaaaatca gcagtacaga
     8161 aaccagaata ttggctccac ggaaaagaac actgcgcaga cgcgaggtgg caggtttgat
     8221 ttttagccag caggcaatca gcgcagcaag cacgccgctg aatcccagcg ttaataacat
     8281 ttgcggtgtc atcagcgcat aactttctgc tggagtggta tcaataatat tggcaatcat
     8341 cgagcggtcg atgacgatgc cgtaagtcat tatgaaatat tgtgcagccg cgccaatcag
     8401 aataaacagg caggccagtg gtcgattaag ccataagaaa gagcttagtg tcaggacaat
     8461 attaatcacg ctgaaagcga cgaccggcat cgacaagaaa accagtacgt tatgcagcga
     8521 atccagcggc agcgcctgca acacctgttt aaaaaaggca atattcaggc agatagagat
     8581 ataaaaagcg gccaacaata gccaggcgag taaattcaaa gagggtcttt ttagtaggcg
     8641 cttcaacatc acggtgtttc catcgaacaa agtgcgcata tgctcgcaaa gcaaaattaa
     8701 gccaacctta agttcttaag gttggctttt atgtttgctg gattaagggg aattaatctt
     8761 tgcttattgg tgcatctaag ggatacgggt ttttatgtag ccggttgtaa ttcagcgcat
     8821 acatggcggt gatgaccatc agggtgacaa atgaccacat aacctctttc gccccggacc
     8881 ccaccacggc ccaaatgcag tagaggaagg caatggtagt aacagccaga tatgccgggc
     8941 gtgctttacc aaagtgaccg tggccgagca gcagtaacgc cgcacaggtg taaagatatg
     9001 gcaccagtgt aaagatgacc gagacggaag aaaccagacc gaactctttg gtcgcgtttg
     9061 gtgaaatgct gctgagctgg aagatggtca tcaaaatacc gacgataatc agccccgcca
     9121 ctggcgtacc cgctttattt acacgggcaa aaatcggtgg gaacagtccg tcatcggcag
     9181 cggctttcgc cgtttgaccc gccagcaacg tccagccgcc cagtgaacct aagcaacccg
     9241 cagctgcgca gaaggaaaca atggccccag cggtgtcacc caacgccatc cgtgcggcat
     9301 caccgaatgg cgaggcagaa acgcgcagtg cggcattagg gatcatcccc ataatcgcgg
     9361 tggtggagag cacatagcaa acggcggcaa tcaatacccc accaatggtg gcgataggga
     9421 cattgcgttt cgggtttttc accacacctg cggcaacgga ggcactttcc acaccgatga
     9481 acgaccacag cgtaacgtta agggtacttt gaatcgcacc gaaggtgccc aggccgctga
     9541 cgttccatgc cgccatatag gtttcaccac ggaaccagaa ccagccaaat acggcaatcc
     9601 cgacgatggg gatcagcgcc agcacagtgg caacggcctg cacgcgggtg atcattttcg
     9661 gaccgacaat gttcagcagg acgaagatcc acagcaccac gacgcaggtg atggttaata
     9721 ccaatggatc tttcagaatc gggaagaagt aacttaaata tcctacgcca atgaccacca
     9781 tggcgatatt gccgatccag caggccagcc agtagaggac gttggtttga taaccgagaa
     9841 acgggccaaa gcagcggcgg gcgtaagcgt aagaaccacc aggacttggg tcgaggaacg
     9901 acattttggc gtataccatc gagagcccca gcgcaccgat aatcgtcacc aaccatccat
     9961 aaatggcaat cccgccagtg gaggccaggt ttgcaggtaa cagaaaaaca cctgacccca
    10021 taatattccc cgacaccatc agggtgacgg ggattaagcc cactttgtga gcatcagcat
    10081 ccgaagacat aattaaactc ctgcgaaggc gagcttcgtg acaataaatt acgtcatatc
    10141 atacgcctgc attcgatagt tgaattatta tcgaccgggt tatcgctgat gccgttatta
    10201 atatagctct tcgttaaggt taaaaatcag gcgacagtgc gttctctgtg ctgactaaca
    10261 taatgcagcg gcgtcatacc gtagaagtct ttaaagacag aaataaagta cgacgtactg
    10321 ttgtagccgc aggactgtga tacctgagag atatttttac cgtccatcat taattcattt
    10381 acggcataac gcatgcggca ggtggtgatt atttggctat aactggtgtt ttcgcttttc
    10441 aactttttct ttaacagact tgggctaaga cataaacaac tggctaccat actaagattc
    10501 cagtctttgt gaatatcgct ttcaataatt tgataaacgc tgtcacttac acaattacgt
    10561 aacatataca tcattaatga aaggaatttt ttactgtcga gaaaacggga tagcaccgtg
    10621 aataataatg cgcgtgttcg ttccttttct gactcagttt ctgcgggcat catgctgtgt
    10681 tgcgcggcaa cgcgaaagac gtctggcgtc aggcatggca gggtgagtat gggcgtagcg
    10741 ctacgttgcc atactggtat ttgcgagaga tctttattca gaaaccggag gtaatctttg
    10801 atgatgtcgt gactaatatg agcaaccaaa gtgttgttaa gagaggagac gtcgataata
    10861 ttattttcac aatttaataa cgccatatgg ttagctttaa ggctaatagg ttctttccca
    10921 ttcaccctta tccagacatc tttttcagtc aataaaacaa tacaaggttg gtcgctgcaa
    10981 atcctcatac atgtactcct gagtgcgaat aaaagaccag ttctcgctaa agcaaagcga
    11041 taccgggaaa tggttatata attgcatgat gaatataaac atacataaaa ataacgagtt
    11101 ttactaatat tacaaatttt tatagtaatt tctcatgtac atattttttt acaggataaa
    11161 taacaggcaa aaaaaagcgc ggggtggggc cgcgcaaaaa gtatgacatt tttgtttgca
    11221 gcaattttta tgaaaacgtt gccatactca aaaggcctaa ggccaggcgg cgtaatgtta
    11281 tttaaacaat tacgccttca gcggaatagt ggttacgctt tcacgcacat aacgtggtaa
    11341 ataccgtcaa taatttcagt cccttcagtt tcgtgttcaa atccagggaa atggtggtcc
    11401 caggattgca gcgagcgtaa ataacttact tgcggactgt ttttatcgcc gaagttttca
    11461 ccagacagca gcatcgggat tcctggcgga tacgggataa ctgagtttgc cgcgatgcgt
    11521 cctggcagat tttcaatgga taccagttcg acattgttgt cgacaatcgc gttgtacgct
    11581 tcacgcgggg tgatttccgc caccggcagg ccggaatagg cttcgttcaa ccgtgcgcca
    11641 gggttgtttt ctttcagcca ggcaaacatg gtgtcaccca gatcgtgaat ccccatgttc
    11701 gcgtaagtgt caggatattg ttcaacaagt tccggcatca cctgcgccag cggtgtgttg
    11761 gcgtcatagt ggtgtttgaa ggagcaaagg gtgttaacca gagtccccca tttcccacgg
    11821 gttacgccca tagagaacag gaacataatt tggaagtcag tggtgcgggt aggcacaatg
    11881 ccgtggcgac caagccaggc agtgaccagc gccgccggaa caccggtttc ttccagttca
    11941 ccatcttcac ccattcccgg agcaaggatg ctgactttaa ttgggtcgag catactccag
    12001 ttatccggaa tatctttgaa gccgtgccag ctttcgcccg gatgcattac ccagcagtcc
    12061 tgaacggtgg tcagcagttt ggttggtgcg tcagcaaagt cataggtttt gccggtttgt
    12121 gggtcggtga cgacttcttt gttccacggt ttgaagaacc agctaccgtc agcggtgaac
    12181 tctttatata gccgcgccat cgcctgacgg aaatcaaccg cttcgtcaat cacttcctgt
    12241 gtcagtgaca ggccgctgtt gccgtccatc atcgacaccg ccacgtcgtt ggatgcgcag
    12301 atggcataca gcggggaggt ggtggcatgc atcatgtagg cctggttgaa gcgggagaag
    12361 ttaatcgccc cacgaccttc acgtacatga atataagaag cctgtgacag cgcattcagc
    12421 agtttgtgga tggagtgggt ggcgaaaacg gtaggaccgt tgtgatcgcc aggttcgccg
    12481 cgcatggcat agtgatcggc atagatcggg ttgaaacgtg catagccgta ccaggcttcg
    12541 tcaaagtgca gacgatcgga ggttttttcc agcagatcct gcgcttcttt agcgttataa
    12601 cacacgccgt cataggtgca gttggttacc acgcagtaag acggtttttg cccggctttg
    12661 tctttggtca gcgggctttc actgattttc ttttgcaagg tttcaggttg catttcctgc
    12721 ggatagattg gcccgataat gccgtagcgg ttgcggcttg gcaccatata gaccggtttc
    12781 gcgcctgtca gcatcaaacc ttgttcgatg gatttatggc agttacggtc aacgaccacg
    12841 acatcgttat cggtcatgca agcctgcatg atggtgcggt tagagccgga agtaccgacg
    12901 actaccgacc aggagcgatc ggcaccaaat acgcgtgcgg catatttttc gctttcgcca
    12961 aatgcgccag tatggtcaag caaagaaccg agggaagttc gttcgatgcc catgtcggtg
    13021 cggaacagat tttcaccata gtagtcatgg tagaaacgtc cggcgggtgt tttggtaaaa
    13081 ccaacgccgc cctggtggcc tggcgctgcc caggaatatt catggatgtc actatatttc
    13141 atcagcgcgc tgaacagtgg cggcaacagc tgctggcggt agcgggtcat cgcggcaacg
    13201 gcgcgtccgg cgataaagtc ggcggtatct tccagaatcc aggcgaattc atcgacaagc
    13261 tccagcagat cccggtccat tgcggcgagg gctttttccc gatcgcccaa caggaagacc
    13321 ggcacgtttt gttggcgctc atgaagctta ccgatcaatt gtctgacgtt ttgatgttcg
    13381 tccggatgct ccatttgata gctgaacatc aggcagtcaa tggcttcgtt tgaagagaga
    13441 atggcaaaac catcatcaaa ggaggtggat ttaatcacgg taacattttg ctggcttaaa
    13501 gcatctgcca gacgctcaac ggcgttaccg acccaggtgt cttgatggag aaactcgctt
    13561 tcaacaatta atactttcat cattgcttac ccggttatga aggaatcttc gttgcgggca
    13621 agtatcttcc ggccttttcg ggctgtaaag cgcgtgaaaa acaaacattt ttgaaatatt
    13681 tagttttata aacaatacgt tatgtgatta ttttaaatta ttttcactgc ttattatcga
    13741 cggctaaact atttttttgg ctgatactga tatcgtcttt agccgggaaa cgtctggcgg
    13801 cgctgttggc taagtttgcg gtattgttgc ggcgacatgc cgacatattt gccgaacgtg
    13861 ctgtaaaaac gactacttga acgaaagcct gccgtcaggg caatatcgag aatactttta
    13921 tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca tcgcggtaat gtactgtttc
    13981 atcgtcaatt gcatgacccg ctggaatatc cccattgcat agttggcgtt aagtttgacg
    14041 tgctcagcca catcgttgat ggtcagcgcc tgatcatagt tttcggcaat aaagcccagc
    14101 atctggctaa cataaaattg cgcatggcgc gagacgctgt ttttgtgtgt gcgcgaggtt
    14161 ttattgacca gaatcggttc ccagctagag aggctaaatc gcttgagcat caggccaatt
    14221 tcatcaatgg cgagctggcg aatttgctca ttcggactgt ttaattcctg ctgccagcgg
    14281 cgtacttcaa acgggctaag ttgctgtgtg gcaagtgatt tgatcaccat gccgtgagtg
    14341 acgtggttaa tcaggtcttt atccagcggc caggagagaa acagatgcat cggcagatta
    14401 aaaatcgcca tgctctgaca ggttccggta tctgttagtt ggtgcggtgt acaggcccag
    14461 aacagcgtga tatgaccctg attgatattc actttttcat tgttgatcag gtattccaca
    14521 tcgccatcga aaggcacatt cacttcgacc tgaccatgcc agtggctggt gggcatgata
    14581 tgcggtgcgc gaaactcaat ctccatccgc tggtattccg aatacagcga cagcgggctg
    14641 cgggtctgtt tttcgtcgct gctgcacata aacgtatctg tattcatgga tggctctctt
    14701 tcctagaata tcagaattat ggcaggagtg agggaggatg actgcgagtg ggagcacggt
    14761 tttcaccctc ttcccagagg ggcgagggga ctgtccgagc atcatgaggc cgaaaactct
    14821 acttttcagg taatttattc ccataaactc agatttactg ctgcttcacg caggatctga
    14881 gtttatggga atgctcaacc tggaagccgg aggttttctg cagattcgcc tgccatgatg
    14941 aagttattca agcaagccag gagatctgca tgatgtctgc acccaaaatt acatttatcg
    15001 gcgctggttc gacgattttc gttaaaaata ttcttggtga tgtgttccat cgcgaggcgc
    15061 tgaaaacggc gcatattgcc ctgatggaca ttgaccccac ccgcctggaa gagtcgcata
    15121 ttgtggtgcg taagctgatg gattcagcag gggccagcgg caaaatcacc tgccacaccc
    15181 aacagaaaga agccttagag gatgccgatt ttgtcgtggt ggcatttcag attggcggtt
    15241 atgaaccttg cacggtgact gatttcgagg tctgtaagcg gcatggtctg gaacaaacca
    15301 ttgccgatac gttggggccg ggcggtatta tgcgcgcgct acgtaccatt ccgcatctgt
    15361 ggcaaatttg cgagaacatg acggaagtct gccccgatgc caccatgctc aactatgtta
    15421 acccaatggc gatgaatacc tgggcgatgt atgcccgcta tccgtatatc aaacaggtcg
    15481 ggctgtgcca ttcggtgcag ggaacggcgg aagagctggc gcgtgacctc aatatcgacc
    15541 cggctacgct gcgttaccgt tgcgcaggta tcaaccatat ggcgttttac ctggagctgg
    15601 agcgcaaaac cgccgacggc agttacgtga atctctaccc ggaactgctg gcggcttatg
    15661 aagcagggca ggcaccgaag ccaaatattc atggtaatac tcgctgccag aatattgtgc
    15721 gctacgaaat gttcaaaaag ctgggctact tcgtcacgga atcgtcagaa cattttgccg
    15781 aatatacgcc gtggtttatt aagctaggtc gtgaggattt gattgagcgt tataaagtac
    15841 cgctggatga gtacccgaaa cgctgcgtcg agcagctggc gaactggcat aaagagctgg
    15901 aggagtataa aaacgcctcc cggattgata ttaaaccgtc acgggaatat gccagcacaa
    15961 tcatgaacgc tatctggact ggcgagccga gtgtgattta cggcaacgtc cgtaacgatg
    16021 gtttgattga taacctgcca caaggatgtt gcgtggaagt agcctgtctg gttgatgcta
    16081 atggcattca gccgaccaaa gtcggtacgc taccttcgca tctggccgcc ctgatgcaaa
    16141 ccaacatcaa cgtacagacg ctgctgaccg aagctattct tacggaaaat cgcgaccgtg
    16201 tttaccacgc cgcgatgatg gacccgcata ctgccgccgt gctgggcatt gacgaaatat
    16261 atgctcttgt tgacgacctg attgccgccc acggcgactg gctgccaagc tagttgcacc
    16321 gttaaaacgc gactaaacgc tactgcgccg ggggatttat tccggcgcac acctctgacg
    16381 ataccaataa cagaaggcgg gcgttggtaa cagcgacccg ataccctatg agcatttcaa
    16441 tgactacaaa actcagttat ggatttggag cgttcgggaa ggattttgcg atcggcattg
    16501 tgtatatgta cctcatgtat tactacaccg atgtcgtcgg gctgtctgtg ggtttggtcg
    16561 gtactttgtt tctggtggcg aggatctggg atgctattaa cgatccgatt atgggatgga
    16621 ttgtaaatgc tacgcgatcg cgatggggta agttcaaacc ctggatcctg atcggtacgt
    16681 tggcaaactc tgtaatctta tttctcctct ttagtgcgca tctgtttgaa ggtactactc
    16741 agattgtctt tgtttgcgtg acctacatcc tctggggcat gacttacacc attatggata
    16801 ttcccttctg gtcgctggtt ccaaccatca cgctcgataa acgtgaacgc gaacaactgg
    16861 ttccttatcc gcgttttttt gccagtctgg caggctttgt tacggcaggt gtgacgctac
    16921 catttgttaa ttatgtcggc ggtggcgatc ggggatttgg ctttcagatg ttcactctgg
    16981 tattgatcgc cttttttatt gtttcaacca tcatcactct gcgcaatgtg catgaagtct
    17041 tttcgtcaga caatcaatcg tctgctgaag gaagccatct gacacttaaa gccatcgttg
    17101 cgcttattta taaaaacgat cagctttcat gcctcttggg tatggctctt gcttataatg
    17161 tagccagcaa cattattacc ggatttgcta tctattattt ctcatatgtt atcggtgatg
    17221 cggatttgtt cccctattat ctgtcgtatg cgggagctgc taacctggtg acgttagtat
    17281 tcttcccacg cttagttaaa tcattatccc gacgcatttt atgggccgga gcatctattc
    17341 ttccggtgtt aagctgtggt gttctcctgt taatggcatt aatgagctat cacaacgtcg
    17401 tcctcattgt gattgcgggt attttgctga atgtgggaac ggcgcttttc tgggtattac
    17461 aggtcatcat ggtggcagat accgttgatt acggtgaata taaactgcac gtacgctgtg
    17521 aaagcatcgc ttactccgtg cagactatgg tggtgaaggg cggttcagcc tttgcggctt
    17581 ttttcattgc ggttgtgtta gggatgattg gctatgtacc gaatgttgaa cagtctacgc
    17641 aagccctatt aggtatgcag tttattatga ttgctctacc gactctgttt ttcatggtaa
    17701 cgctgattct ctacttccgt ttctatcgcc tcaatggcga cacgctgcgc aggatccaga
    17761 ttcatctgct ggataaatat cgcaaagtac cgcccgagcc tgttcatgct gatattccgg
    17821 tcggtgcagt gagtgatgtg aaagcctgac gtggacaata gctaacgaaa atggcctgat
    17881 gcgatatgtt tatcaggcca cgtcttaatg taatgctttg gatttgcaaa ttttgtagac
    17941 cggataagga attcacgccg catccggcat caacaaagtg cacgttgtta tccagttatc
    18001 aagccatagc gccgtagctg gcggcaatga aagcgagcga gcaatatcac tgtcgttaat
    18061 gcgccaagca gcgcgcagaa catatcggat tgcgtgtccc actggtcgcc ctgggtaccg
    18121 agaaaatcat ccgctccctg gcccatcgcc agtgccgccc accactcgat taattcatac
    18181 atcgcgctta tcgccagcgc cacgcaacag accagaaacg ccaccatttt acgtccgcgc
    18241 acgtacatcc cgcgaacgag aatttctcgt gccaccagtg caggcaccag cccctggaaa
    18301 aagtgcccca gcttgtcata cggattacga ctcaacccca gccattcctg cacctcaaaa
    18361 ccaacgggga ctttcgcgta ggtgtactgc ccgcctacca tcaggatgat ggcgtgaaag
    18421 aagatgagcg tatagagcag cagcgttaac gggtaacgtc tggcggtggc aagtagtagc
    18481 ggcacgacga taatcaccgg tgtcacttcc agcagccagg tgagtttgtc atgggccgaa
    18541 atgccggtat aaatcaggat taacgttagc gccagtgcgc cggtgttaag aattaacggc
    18601 ttgagtgtgc gggtcatggt gagttcgcaa gtcagggaaa atactgacta ttcaccagca
    18661 agcatgaaat tacaatcgca aaagaggtgg cccaggggga tcacctggca gcatgctgtc
    18721 aggcgctggg ccgaagaggt tacttagtgc agttcgcgca ctgtttgttg acgatttgct
    18781 ggaagaagtc gttacctttg tcatcgacca ggataaacgc cgggaaatct tctacttcga
    18841 ttttccagat agcttccata cccagctccg gataagcgac gcattccaga tgcttgatgc
    18901 tctgctgcgc cagtaccgcc gccggaccgc cgatgctacc gaggtagaag ccgccgtgtt
    18961 tatgacacgc gtcggtaacc tgctggctgc ggttgccttt cgccagcatg atcatgctgc
    19021 cgccgtggga ttgcagcaga tccacatagg agtccatacg acctgcggtg gttgggccaa
    19081 gtgaacctga tggataaccg gcaggggttt tcgccggacc cgcgtagtag atcgggtgat
    19141 ctttgatgta ctgcggaagt tctttaccgg cgtcaatcag ctctttcagc ttggcgtgtg
    19201 caatatctcg acccacaata atggtgccag tgagcgacaa acgagtggaa accggatatt
    19261 gcgaaagctg ggcgaggatc tctttcatcg ggcggttaag gtcgaccttc accgcttcgc
    19321 cttcaccggc ctgacgcagt tcttgtggaa tgtactgacc tagattgtgt tccagttttt
    19381 cgatccagat accttcgcgg ttgattttcg ctttaatgtt acggtcagcg gagcaggaga
    19441 cgcccatgcc taccgggcag gatgcgccgt gacgtggcag acggataacg cgaatgtcgt
    19501 gcgcgaagta tttaccgcca aactgcgcgc caagaccgag tttctgggcc tcttccagca
    19561 gttcctgttc cagctggaca tcgcggaacg cctgaccatg ttcgttccct tccgtcggca
    19621 gttcatcgta atagtgagcg cttgctaatt tgacggtttt caggttggtt tccgcagacg
    19681 taccgccaat cacaaacgcg atatggtacg gcgggcaggc tgcagtaccg agggtacgca
    19741 ttttctcgac gaggaagttt ttcagtttgc cgggagtcag cagggctttg gtttcctggt
    19801 agagatacgt tttgttggca gagccgccgc ctttcgcaac gcaaaggaat ttgtactcat
    19861 cgccatctac cgcgtacagg tcgatttgcg caggcaggtt agtgccggtg ttgacctctt
    19921 tgtacatgtc cagcgccgcg ttttgcgagt agcgcaggtt atcttcgata taggtgttat
    19981 agacgccttt cgacagcgct tcttcatcac cgccaccggt ccacacgcgc tggccttttt
    20041 taccgacgat gatcgcggtg ccggtatcct ggcaggtcgg cagcacgcct ttggcggcga
    20101 tttcggagtt tcttaagaat tgcagtgcca cgtacttgtc gttttcgctg gcttctggat
    20161 cgtgaagaat agccgcaacc tgtttctggt gtgccggacg tagcatgaaa gaagcgtcgt
    20221 gaaaggcttg ctgcgccagc agggtcagtg cttctggttc cactttcagg atggtttcgc
    20281 cgtcgaagtc ggcaacgcta acgtaatcgg aagtgagtag atagtattcg gtattgtctt
    20341 tccccatcgg gaaaggtgcc tggtagataa agggtttgtt tgacatagct tccagcctgt
    20401 aactctgtat ttgttattcg aaaatggcgt gccgcgtagt cacgggcggc acgcaaagtg
    20461 catttataag aacccgtaca tcgcggcgaa gatccagccg aagacgcacg atacgctcac
    20521 accaatcaac cccggcagaa taaaactgtg gttgatgacg aagcgaccga tgtgggtggt
    20581 gccggaacgg tcaaactgaa tcgctgccag atcgctcgga taagtcggca ggatgtaata
    20641 accgtagcaa gccggtgctg aagccacgat gtatgccgga tcaacgccga tcgccagcgc
    20701 gaccggaaca atcgccgcca gcgccgcagc ctgagagttt acaaacttgg aaaccagcag
    20761 cagaacaatg gcatacgccc acggatactc tttcaccatt tcacccagta cgccctgaat
    20821 ttcagacata tgcgcaccga acatggtttc tgccatccat gcgataccgt acaccgccac
    20881 gatggcgatc ataccggaac ggaagacttc gttttttgag atagacgcgg gattggtttt
    20941 ggtcaggata ataatcagcg ccccggtcag cagcataaac atctgaataa ccagtaccat
    21001 cgacagcggt ttgccgccga aggatggacg caggtccgaa tcagcaccaa gaagggcgac
    21061 tacagcgatt gccccgagga aaatccacat tgccagccag ttgcttttcg gcagtttttt
    21121 atccagcagc gtcgcggtat caccgtaaac atactcacgg ttttccggta cggagatgaa
    21181 tttctggaac tcttcgtctt tatccagatc tttaccgcgg aaccagctga agataccgat
    21241 cgccaggata ccgattaacg tcgatggaat ggtgattgcc agcagatcga ggaactcaag
    21301 atggcgacca tcaaaggtga cattacccag catcgcaacc agagacacga ccgcaaccga
    21361 caccggactg gcgataatcc ccatctgtgc accgatagaa cttgccgcca tcggacgttc
    21421 cggacggatg ttgttcttaa tggcgacgtc gtagatgatc ggcagaatgg tgtaaaccac
    21481 atgacccgta ccgcaaagaa tggtcagtgt acaggtcaca aacggcgcga caattgagac
    21541 atatttcggg ttgcggcgca gcagcttctc ggcaatttgc agcatgacat caagaccgcc
    21601 cgaagcttgc aaggtcgccg atgccgccac caccgcaatg ataaccagca tgacatcaac
    21661 tggtggttta cctggctgaa ggtggaagac gaagaccaga atgaccagac cgataccgcc
    21721 taataaaccc agcgcgatac caccctttct ggcaccataa aacagacata ttagtattat
    21781 gataagttgg atagtaaata acatgtgtga accctcgcga taatcctatt taaatttttg
    21841 ctgaatagat cacagtcacg ttctgttttg tatgaactgt tttcagagcc tgactaaata
    21901 tccgtttggt cgttaccggc tttagcaaat acctcacagt gaatattggc tgattaaagc
    21961 agcggcaaat ttatgcactg tgataagcgg ctttttcaga gagaaaaact ctctggcgat
    22021 gggtttatag tttgtactgc gcagatgtta atgttttgtt tgctgttatc ttattgatat
    22081 ttatcggtct attttttgtt ctcagggcag atatcattag gtttatagat taatctgatc
    22141 tacccatttg tgggtaaaaa tacacataat gcgggtgaca taatagttaa ttaacttttg
    22201 ttatcgtttt gaaattaaaa acaccgttca tctgaagaga tattaatttt taacgatgat
    22261 ggagggataa ttatatttga tctggcacaa gttttactga ttaacgatgt aacttgtgcc
    22321 aggggtattt tgcattacgg taataattat tacttacaga taaccagcga tccgttattg
    22381 gcaatattgt ttcagcagtg agtagtgttc tgcctgaata cggtaacggt aaaccggacg
    22441 ccccgtgacg ccataatgga tactggtgaa caagatgtgg cagttgacca gccagatgag
    22501 gtattttcgg caggaaacac gcgagatgtt aacctcgtta gccagctcgt cggttgaaaa
    22561 ctcatagtcc tgatgcgcgt caatccactg gcacagcgtg cgtaacgtct gcggcgttaa
    22621 gccttttggc aagcgacgag gatcctgttc gttggagctg ctgccgtgga ttagctgatc
    22681 aagctcggcc tggtcataat actgatgttt ttccagcgcc attttctttt gccgccagcc
    22741 ggtgagcgcc tcttcaaagc gggaagcctg gaagggtttg atcaggtaat ccacgacacc
    22801 gtaatgcagc gaatctttaa tggttgccgc atcggctgcg gaggagatga caatcacatc
    22861 acttttgcaa cgcgcgttat gcaggacagg cagtaaatcg agcccgttct ctttttgcat
    22921 atagatatcg agcaatatca ggtcgatagg cgcatcgcta ttgaagataa tctctttggc
    22981 tttctccagc gtcgaggctg ttccacagca ttgaaagcct gggatttgtg ctacgtatcg
    23041 gcgattcagc tccgcgacca ttgcgtcgtc atcgataatt aatacattga tcatctgttc
    23101 gacctctccc cgtcccaggg tatctggaca aaaaattgtg tgaaaatccc cggttccgat
    23161 tccacggcga tgctgccgcc gagattttct acctgttgtt tgacaagtgc taaaccgacg
    23221 cctcgctcgc ttccttttgt cgagacacct ttgtcaaaaa tgtgatcgat tttgtcgggt
    23281 gcgatccccg gtccatcatc gttaacttcg cagtgcagcc agccgtgacg gtagtgcaat
    23341 gttacgctaa tttcgccacc gggttccggc cctaatgctt ccagcgcgtt ttctatcaga
    23401 tttcccaatg tggtaatcag cgtcgcgacc tggtcctcac tgccgctgtc tggcagctgg
    23461 ctttcactgt ttaaaatcag cgtatggcct aaatcggtcg cgcggttaat cttgctgatt
    23521 aaaaaaccag cgataaccgg agatttgatc ttacccagca gagagccaat ctcttcctga
    23581 tagttattgg ctgttttgag aatgtaatct tccaactgct tataactctt cagatgcaat
    23641 aatccgagaa tcacatgcaa tttattcata aattcgtggg atcgttcacg aagtgcgtca
    23701 gcatagttga ccagaccgtc gagtcgctgc atcagtttac gaacttcagt tttgtccctg
    23761 aaggttgaaa tggcaccgat gataacgcca ttactgcgca ccggaacggt gttgatcagt
    23821 aatagccggt ctttaatcgt aatctcttcg tcgcggcgcg gcgtaccgtc gcgtaacact
    23881 tccgagacat ctaccacctg tgaccatgag tggctgagcg tcgacagttt ctcatcgtcc
    23941 tgcgacttac ggtaattcag caattcttgt gcggcatcgt tgatcagcgt gacctcgccg
    24001 cgatcgtcca cggcaacgac gccttctttg atagactgca acatggcctg gcgttgctca
    24061 aacagcgtgg agatttcgta gggttccagg ccgaaaagga tttttttcag taccttaacc
    24121 agaatgcagg tgccaatcag tccgaccagc atgccaaata ataccgacca gataatgctc
    24181 cagcgactgt cattgatctg ttgggtcacg cggcttaact caaggccgat cgccaccacg
    24241 ccaatttgtt tatgattttc atcgtagatg ggggtaaata cgcgtaaagc ctgcgccaga
    24301 aaaccgcgat tgatagcgac attttcttcg ccattcagcg cattaaggat gtcatcacct
    24361 ttaaatggct gaccaatacg ctgggcttca ggatgtgagt agcgaagact ttgcatatcg
    24421 gtaacgacaa taaacagcag atcgttgcgt ttgcgcacgg cttccgcgat ggcctggatg
    24481 ccactctcct gcggtttttt ctgcaagccc tgacggattt ccggcgagtc ggcgagggta
    24541 cgcgccactg ccagtgcctt gttggccagc ccgtctcgcg tcatatcact gatttgcgag
    24601 aagtaaatca gatgcaccac caatagcacc gagaacagta ccgcgctgac cattaagatc
    24661 acagtggtac tcaatttcat cggacgtttg cgtaatatgt ggtagggcaa tgaatgtctc
    24721 atcagcttcc ttgtgtgaca aatttcttaa gcattatctc tgatgaggcg ggtaattcaa
    24781 agggagtaaa aatgattggc tatatagggg aagagactct ggcaacggaa actgccagtg
    24841 ctgtatgaag attccggggc tatgcttata gcgataatca tactgatgag agagggaagg
    24901 tcatggatca ggcgctactg gacgggggtt atcgctgtta taccggcgaa aagatcgatg
    24961 tctatttcaa cactgcgata tgtcagcatt ctggcaattg cgtacgtggc aacggcaagt
    25021 tatttaatct caaacgaaag tcg
//
LOCUS       DAJTUN010000054        24517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTUN010000054 DAJTUN010000000
VERSION     DAJTUN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.054"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            11..1384
                     /gene="ravA"
                     /locus_tag="OO470_002040"
     CDS             11..1384
                     /gene="ravA"
                     /locus_tag="OO470_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6251288.1"
                     /translation="MFLLGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGP
                     LSIQALKDEGRYERLTSGYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVE
                     KIPMRLLVAASNELPEADSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPV
                     PDALQVTDEEYERWQKEIGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRL
                     LQASAFFSGRSAVAPVDLILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLG
                     AIVQRHLQLQQQQSDKTALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDM
                     EVVHISFERSALEQWLSKGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLA
                     LPGSSAEGLPGEIKQQLEELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQ
                     IRQAQQC"
     gene            1378..2829
                     /gene="viaA"
                     /locus_tag="OO470_002041"
     CDS             1378..2829
                     /gene="viaA"
                     /locus_tag="OO470_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6251289.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2834..3826)
                     /gene="asnA"
                     /locus_tag="OO470_002042"
     CDS             complement(2834..3826)
                     /gene="asnA"
                     /locus_tag="OO470_002042"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6251290.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            3978..4436
                     /gene="asnC"
                     /locus_tag="OO470_002043"
     CDS             3978..4436
                     /gene="asnC"
                     /locus_tag="OO470_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6251291.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4526..4969
                     /gene="mioC"
                     /locus_tag="OO470_002044"
     CDS             4526..4969
                     /gene="mioC"
                     /locus_tag="OO470_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6251292.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5348..7237
                     /gene="mnmG"
                     /locus_tag="OO470_002045"
     CDS             5348..7237
                     /gene="mnmG"
                     /locus_tag="OO470_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6251293.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7301..7924
                     /gene="rsmG"
                     /locus_tag="OO470_002046"
     CDS             7301..7924
                     /gene="rsmG"
                     /locus_tag="OO470_002046"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6251294.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8541..8921
                     /gene="atpI"
                     /locus_tag="OO470_002047"
     CDS             8541..8921
                     /gene="atpI"
                     /locus_tag="OO470_002047"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6251295.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            8930..9745
                     /gene="atpB"
                     /locus_tag="OO470_002048"
     CDS             8930..9745
                     /gene="atpB"
                     /locus_tag="OO470_002048"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6251296.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9792..10031
                     /gene="atpE"
                     /locus_tag="OO470_002049"
     CDS             9792..10031
                     /gene="atpE"
                     /locus_tag="OO470_002049"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6251297.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10093..10563
                     /gene="atpF"
                     /locus_tag="OO470_002050"
     CDS             10093..10563
                     /gene="atpF"
                     /locus_tag="OO470_002050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6251298.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10578..11111
                     /gene="atpH"
                     /locus_tag="OO470_002051"
     CDS             10578..11111
                     /gene="atpH"
                     /locus_tag="OO470_002051"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6251299.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11124..12665
                     /gene="atpA"
                     /locus_tag="OO470_002052"
     CDS             11124..12665
                     /gene="atpA"
                     /locus_tag="OO470_002052"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6251300.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12716..13579
                     /gene="atpG"
                     /locus_tag="OO470_002053"
     CDS             12716..13579
                     /gene="atpG"
                     /locus_tag="OO470_002053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6251301.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13606..14988
                     /gene="atpD"
                     /locus_tag="OO470_002054"
     CDS             13606..14988
                     /gene="atpD"
                     /locus_tag="OO470_002054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6251302.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15009..15428
                     /gene="atpC"
                     /locus_tag="OO470_002055"
     CDS             15009..15428
                     /gene="atpC"
                     /locus_tag="OO470_002055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6251303.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15779..17149
                     /gene="glmU"
                     /locus_tag="OO470_002056"
     CDS             15779..17149
                     /gene="glmU"
                     /locus_tag="OO470_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6251304.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17311..19140
                     /gene="glmS"
                     /locus_tag="OO470_002057"
     CDS             17311..19140
                     /gene="glmS"
                     /locus_tag="OO470_002057"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6251305.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19300..20121
                     /locus_tag="OO470_002058"
     CDS             19300..20121
                     /locus_tag="OO470_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6251306.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20108..22216
                     /locus_tag="OO470_002059"
     CDS             20108..22216
                     /locus_tag="OO470_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6251307.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22213..23880
                     /locus_tag="OO470_002060"
     CDS             22213..23880
                     /locus_tag="OO470_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6251308.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            23883..>24517
                     /locus_tag="OO470_002061"
     CDS             23883..>24517
                     /locus_tag="OO470_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6251309.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELS"
BASE COUNT         6238 a         5838 c         6369 g         6072 t
ORIGIN      
        1 tggtgaaagt gtgttcctcc ttggcccgcc aggtattgcc aaaagtttga tcgcccggcg
       61 cttaaaattc gcctttcaga atgcccgcgc gtttgaatat ctgatgaccc gcttctccac
      121 gccggaagaa gtttttggtc ccctttctat tcaggcgcta aaagatgaag ggcgctatga
      181 acgtttaacc agcggttacc tgccggaagc agaaatcgtc tttctggatg agatctggaa
      241 agcgggcccg gcaattctta ataccttgct caccgccatt aacgagcgcc agttccgcaa
      301 cggtgcacac gtagaaaaaa tcccgatgcg tctactggtg gcggcctcca acgagctgcc
      361 ggaagcagac agcagtctgg aagcgttata tgaccgcatg ctgattcgtc tatggttaga
      421 taaagtgcaa gataaagcga atttccgctc catgctgacc agtcaacagg atgaaaacga
      481 caatccggtt cctgatgcct tgcaggtcac agatgaagaa tatgaacgct ggcagaaaga
      541 gattggtgaa attacgctgc ccgatcatgt atttgagctg atttttatgc tgcgccagca
      601 actggataaa ttaccggatg cgccttatgt ctcggatcgt cgctggaaaa aagcgatccg
      661 cttattgcag gccagcgcct tttttagcgg tcgcagtgct gttgccccgg ttgatctcat
      721 tttgctgaaa gattgcctgt ggtatgacgc gcaaagcctg aatttgatac aacaacaaat
      781 tgatgtattg atgaccggtc atgcctggca acagcaaggg atgttgaccc gcctgggcgc
      841 gattgtgcaa cgtcacctgc aactccagca gcaacaaagc gataaaacag ccttaacgtt
      901 aattcgtctg ggcggcattt tcagccgtcg tcagcagtat caacttcctg ttaacgttac
      961 tgcttccact ctgactctgc tgctgcaaaa accgttaaaa ctgcatgata tggaagtggt
     1021 tcatatctcc tttgagcgta gcgcgctgga acagtggctg agtaaaggtg gtgaaattcg
     1081 cggcaaactg aacggtatcg gctttgccca gaaactgaat ctggaagttg atagcaccca
     1141 acatcttgtt gtacgcgatg taagtttaca aggcagtacg ctggcacttc ccggttcatc
     1201 ggctgaaggt ctgccaggtg aaataaaaca acaactggaa gagcttgaaa gcgactggcg
     1261 taagcaacac gctttattca gcgaacagca aaaatgtctg tttatccctg gcgactggtt
     1321 aggtcgcatt gaagacagcc tacaggatgt cggtgcacag attcgtcagg cacaacaatg
     1381 ctaacgctgg atacgcttaa tgtgatgctg gccgtcagcg aagagggatt gatcgaagag
     1441 atgatcatcg cactgctggc ctcaccgcag ctggcggtct tctttgaaaa attcccacga
     1501 ctgaaggcgg caatcactga tgatgttccc cgctggcgtg aggcgctgcg cagtcggctg
     1561 aaagatgccc aagtcccgcc ggaactcacc gaagaggtga tgtgctatca gcaaagccag
     1621 ctcctctcca cgccgcagtt tattgtgcag ctaccacaga tcctggactt actgcatcgt
     1681 ctgaattccc catgggcaga acaagcccgg cagttggttg atgctaacag cacaataact
     1741 tcagcgttac acacgctttt tctccagcgt tggcgtttaa gtctgatcgt gcaagcaacg
     1801 acgttaaatc aacagctatt agaagaagaa cgcgaacaac tgttaagtga agttcaggaa
     1861 cgcatgacgc tgagcggaca acttgaaccg attctcgcag ataacaatac cgcagctggt
     1921 cgtctgtggg atatgagcgc cggtcaactt aaacgtggcg actatcagtt tattgtgaaa
     1981 tacggtgaat ttcttaacga acagccggaa ctgaaacgcc tggcagagca gctggggcgt
     2041 tctcgggaag ccaaatcaat accgcgcaac gatgcgcaga tggaaacctt ccgcaccatg
     2101 gtgcgcgaac cggcgacggt tcctgagcag gttgatggtc tgcaacaaag cgatgatatt
     2161 ttacgtctcc tgccgccaga actggcgaca ctagggataa cggaactgga gtatgagttt
     2221 taccgtcggc tggtggaaaa acagttgctc acctatcgcc tgcacggtga gtcgtggcgt
     2281 gaaaaagtgc tcgaacgtcc ggtggtacat aaagattacg atgaacagcc gcgcgggccg
     2341 tttattgtct gtgtggatac ttccggctca atgggcggct ttaatgaaca gtgtgcgaaa
     2401 gcgttctgcc tggctttgat gcgcattgct ctcgctgaaa accggcgctg ttatattatg
     2461 ctattttcca ccgagatcgt ccgttatgag ctttcaggcc cacaaggcat cgaacaagca
     2521 atccgttttt taagccagca gtttcgtggc ggcaccgatc ttgccagttg ttttcgcgcc
     2581 attatggaac gcttgcaaag cagggaatgg tttgatgccg atgcggtggt gatttctgat
     2641 tttatcgctc agcggttgcc tgacgacgtg acgagtaaag tgaaagagct gcagcgggta
     2701 catcagcatc gctttcatgc cgtggcgatg tcggcacacg gcaaacccgg catcatgcgc
     2761 attttcgatc atatctggcg ctttgatacc gggatgcgaa gccgcctgct cagacgctgg
     2821 cggcgataaa ttattacagc agagaaggga cgctctcgcg aacagcagct ggccatactc
     2881 cacactgaac ctggccgata tgcggcagtt gcagcagcag catagtcaaa cgagactggc
     2941 cgataccgcc gccgatggtc tgcggcattt caccgcgcag cagcgcctga tgccattcca
     3001 gctgcagacg atcttcatca ccggtcagcg ccagctgatg cttcagcgtg tcggcatcca
     3061 cgcggatccc catggaagaa agctcaaacg catcttccag taccgggttc cacaccagaa
     3121 tatcgccgtt caggcccgca tggcccagct ctgacggggt gctccagtca tcataatccg
     3181 gtgcgcgcac gtcgtgacga tgaccatcac tcaacttgcc gccaatcccg acgaggaata
     3241 ccgcgccaag atctttcgca atcgcccgct cacgcccttt ggcatcgaga tccggataac
     3301 gagacagtaa ctcctggctg tgtacgaagt gaatctgatc cggcaggaac ggtgccaggc
     3361 caaactcttc gttaaccgca gcttcggttg ctttaattcc cgcccagatc gcctctaccg
     3421 tgcttttcag agtcgagagt tgacgctcac cgtcgcccat tacgcgttcc cagtcccact
     3481 ggtcaacata gaccgagtgc aacggagaaa gacggtcttc atcggggcga agggctttca
     3541 tgtgcgtgta cagcccttcg cccgcgctga agtcgtgttg ccctaaggtc tgacgtttcc
     3601 acttcgccag tgaatgaacc acttcgaact gggcatcagg cagagctttc acttttacct
     3661 gcaccgcttt ttcacagccc gacaagttat cctgcgtgcc atcccccaca cggctaagaa
     3721 tcggcgcctg gacttcgatc agccccagac gttcttccag ttggcgagaa aagtgagatt
     3781 tcacgaagct aatttggcgt tgtttggcaa tgtaagcggt tttcattttt tatactcctg
     3841 cgtcctgttg cttatgatta agcaacaaaa accgacacac atgcaataat cattcaataa
     3901 aaagcctaac acacttttga ttcattaaaa aagacggcta aaatagaatg aatcatcaat
     3961 ctgcataaga aaatcctatg gaaaattatc tgatcgacaa tctggaccgt ggcatcctgg
     4021 aagcattaat gggcaatgcg cgcaccgctt acgccgaact ggcgaaacaa tttggcgtca
     4081 gtccggggac gattcacgtt cgagtagaga aaatgaagca ggcggggatc attaccgggg
     4141 cgcgtattga tgtcagcccg aagcagctcg gttatgacgt aggctgcttt atcggcatta
     4201 tattaaagag cgccaaagac tacccttccg cgctggcaaa gctggaaagc cttgatgaag
     4261 tcactgaagc ctattacaca accggccact acagcatctt tataaaagtt atgtgccgtt
     4321 cgatcgacgc tctccagcat gtacttatca acaagatcca aacaattgat gaaattcagt
     4381 ccaccgagac attgatcgtc ctgcaaaacc cgatcatgcg taccatcaag ccctgatcgg
     4441 cttttttaat cccatacttt tccacaggta gatcccaacg cgttcacagc gtacaatacg
     4501 ccactcttaa taaaggtggc ggtttatggc agatatcact cttatcagcg gcagcaccct
     4561 cggcggtgcc gaatatgtag cagaacacct ggctgaaaag ctggaagagg tgggttttac
     4621 caccgaaacg ctgcacggtc cgctgttgga agatttatct gcctcgggga tctggctggt
     4681 tatcagctcc acccacggtg ccggagatat tccggacaac ctttctcctt tctatgaagc
     4741 attgcaggaa cagaagcccg atctttctgc ggtccgcttt ggcgcaatcg gtattggcag
     4801 tcgtgaatat gacacctttt gtggggctat cgataaactc gaggcagaac tcaaaaattc
     4861 cggtgcaaaa cagacaggcg aaacactgaa gatcaacatt cttgatcacg acattccgga
     4921 agatccggca gaagaatggc tgggatcgtg gattaattta ctcaaataag tatacagatc
     4981 gtgcgatcta ctgtggataa ctctgtcagg aagcttggat caaccggtag ctatccaaag
     5041 aacaaccgtt gttcagtttt tgagttgtgt ataacccctc attctgatcc cagcttatac
     5101 ggtccaggat caccgatcat tcacagttaa tgatcctttc caggttgttg atcttaaaaa
     5161 tcggatcctt gttatccaca gggcagtgcg atcctaataa gagatcacaa tagaacagat
     5221 ctctaaataa atagatcttc tttttaatac ccaggatccc aggtctttct caagccgaca
     5281 aagttgagta gaatccacgg cccgggcttc aatccatttt cataccgcgt tatgcgaggc
     5341 aatcaccatg ttttatccgg atccttttga cgtcatcatc attggcgggg gtcatgcagg
     5401 caccgaggcc gcgatggctg cggcgcgtat gggtcaacag actctgcttt tgacacacaa
     5461 tatcgacact ctggggcaga tgagctgcaa cccggcgatc ggcggtattg ggaagggaca
     5521 tctggtaaaa gaagtggatg cactcggcgg tctgatggcg aaagcgatcg atcaagcggg
     5581 tatccagttt aggatactaa acgcaagcaa aggaccggcg gttcgcgcta cccgagctca
     5641 ggcggatcgt gtgcgctacc gtcaggcggt acgtacggcg ctggagaacc aaccgaacct
     5701 gatgatcttc cagcaggcgg ttgaagatct tattgtcgaa aacgatcgcg tggtcggtgc
     5761 tgttacccaa atgggactga agttccgtgc caaagctgtg gtgctcaccg ttgggacgtt
     5821 cctcgacggc aaaattcata tcggtctgga taattacagc ggtggccgtg ctggcgatcc
     5881 gccatccatt ccgctctctc gccgtttgcg tgaactgccg ctgcgcgttg gtcgtctgaa
     5941 aaccggtact ccgccgcgta ttgatgctcg aaccatcgac tttagcgtgc tggcgcaaca
     6001 gcatggcgat aacccaatgc cggtattctc gtttatgggc aatgcgtccc tgcatcccca
     6061 gcaggtgccg tgttatatca ctcataccaa cgagaaaacc catgatgtga tccgcagtaa
     6121 cctcgatcgt agcccaatgt atgcaggggt gatcgaaggt gtcggcccac gctactgccc
     6181 gtcgatcgaa gacaaagtca tgcgcttcgc agacagaaat cagcatcaga tcttccttga
     6241 accggaagga ctgacctcta atgaaattta tccgaacggt atctccacca gcctgccgtt
     6301 cgatgtgcag atgcaaatcg tccgctctat gcaggggatg gaaaacgcga agatcgtgcg
     6361 tccgggttat gccattgagt atgacttctt cgatcctcgc gacctgaaac cgacgctgga
     6421 gagcaagttt atccaggggc tgttctttgc tggtcagatt aacggcacta ccggttacga
     6481 agaagccgct gcgcaaggtt tgctggccgg tcttaacgct gcccgtctgt ctgctgacaa
     6541 agaaggttgg gctccggcac gttctcaggc gtatctcggc gtactggttg atgacctgtg
     6601 cactttagga accaaagaac cgtatcgtat gtttacttcg cgcgcagaat atcgtctgat
     6661 gctgcgcgaa gataatgcgg atctgcgttt gactgaaatc ggtcgtgaac tgggcctggt
     6721 ggatgacgaa cgttgggcgc gctttaacga gaaacttgag aatatcgagc gtgagcgtca
     6781 gcgtctgaaa tcgacctggg taaccccgtc ggcggaagct gcagccgaag tgaatgctca
     6841 cctgactgcg ccgctttccc gtgaagccag tggtgaagat ctgctgcgtc gtccggaaat
     6901 gacttatgaa aaattaacca cgctgacgcc gtttgcccct gcgttgacag acgaacaggc
     6961 ggcggaacag gttgagattc aggttaaata cgaaggttat atcgcgcgcc agcaagatga
     7021 gatcgaaaag cagctgcgta acgagaacac cctgctaccc gcgacactgg attaccgcca
     7081 gatatccggt ctttctaacg aagtgatcgc caaacttaac gatcacaaac cggcctctat
     7141 cggccaggct tcgcgtattt ctggcgtcac gcctgcggcc atctccattc tgctggtgtg
     7201 gctgaaaaaa cagggtatgc tgcgtcgtag cgcataacgc attaaaaatg cctggtaagc
     7261 acccgcttac caggcaacgc atcaagaaca ggtaatcacc gtgctcaaca aactctcctt
     7321 actgctgaaa gacgcaggta tttcgcttac tgatcaccag aaaaaccagc ttattgccta
     7381 cgtgaatatg ctgcataaat ggaacaaagc gtacaacctg acttcggtcc gcgatcctaa
     7441 tgagatgctg gtacgccata ttctcgatag cattgtggtg gcaccgtatc tgcaaggtga
     7501 acggtttatc gatgtcggca ccggaccagg actgccaggc attccactct ctatcgtgcg
     7561 tcctgaagcc catttcactc tgttggatag ccttggtaaa cgcgtgcgtt tccttcgtca
     7621 ggtgcaacat gagcttaaac tggagaatat tgaaccagta cagagcaggg tagaagagtt
     7681 tccttcagag ccgccatttg atggcgtaat tagccgcgct tttgcctctc tgaacgatat
     7741 ggtgagctgg tgccaccatc ttcctggtga gcaaggccgt ttctacgcgc tgaaagggca
     7801 aatgccggaa gatgaaatcg ctttgttgcc cgaagaatat caggtcgaat cagtggttaa
     7861 acttcaggtt ccagccctgg atggcgaacg tcatctggtg gtgattaaag caaataaaat
     7921 ttaattttta tcaaaaaaat cataaaaaat tgaccggtta gactgttaac aacaaccaag
     7981 ttttctactg atataactgg ttacatttaa cgccacgttc actcttttgc atcaacaaga
     8041 taacgtggct ttttttggta agcagaaaat aagtcattag tgaaaatatc agtctgctaa
     8101 aaatcggcgc taagaaccat cattggctgt taaaacatta ttaaaaatgt caatgggtgg
     8161 tttttgttgt gtaaatgtta tttattaaaa cagtatctgt ttttagactg aaatatcata
     8221 aacttgtaaa ggcatcattt gccaagtaaa taaatatgct gtgcgcgaac atgcgcaata
     8281 tgtgatctga agcacgcttt atcaccagtg tttacgcgtt atttacagtt tttcatgatc
     8341 gaacaggggt agcggaaaag tcgcaattgt atgcactgga aaaatattta aacatttatt
     8401 caccttttgg ctacttattg tttgaaatca cgggggcgca ccgtataatt tgaccgcttt
     8461 ttgatgcttg actctaagcc ttaaagaaag ttttatacga cacgcggcat acctcgaagg
     8521 gagcaggagt gaaaaacgtg atgtctgtgt cgctcgtgag tcgaaacgtt gctcggaagc
     8581 ttctgctcgt tcagttactg gtggtgatag caagtggatt gctgttcagc ctcaaagacc
     8641 ccttctgggg cgtctctgca ataagcgggg gcctggcagt ctttctgcct aacgttttgt
     8701 ttatgatatt tgcctggcgt caccaggcgc atacaccagc gaaaggccgg gtggcttgga
     8761 cattcgcatt tggcgaagct ttcaaagttc tggcgatgtt ggtgttactg gtggtggcgt
     8821 tggcggtttt aaaggcggta ttcttgccgc tgatcgttac gtgggttttg gtgctggtgg
     8881 ttcagatact ggcaccggct gtaattaaca acaaagggta aaaggcatca tggcttcaga
     8941 aaatatgacg ccgcaggatt acataggaca ccacctgaat aaccttcagc tggacctgcg
     9001 tacattctcg ctggtggatc cacaaaaccc cccagccacc ttctggacaa tcaatattga
     9061 ctccatgttc ttctcggtgg tgctgggtct gttgttcctg gttttattcc gtagcgtagc
     9121 caaaaaggcg accagcggtg tgccaggtaa gtttcagacc gcgattgagc tggtgatcgg
     9181 ctttgttaat ggtagcgtga aagacatgta ccatggcaaa agcaagctga ttgctccgct
     9241 ggccctgacg atcttcgtct gggtattcct gatgaatctg atggatttac tgcctatcga
     9301 cttgctgccg tacattgctg aacatgtact gggtctgcct gcactgcgtg tggttccgtc
     9361 tgcggacgtg aacgtaacgc tgtctatggc actgggcgta tttatcctga ttctgttcta
     9421 caacatcaaa atgaaaggca tcggcggctt cacgaaagag ttgacgctgc agccgttcaa
     9481 tcactgggcg ttcattcctg tcaacttaat ccttgaaggg gtaagcctgc tgtccaaacc
     9541 agtttcactc ggtttgcgac tgttcggtaa catgtatgcc ggtgagctga ttttcattct
     9601 gattgctggt ctgttgccgt ggtggtcaca gtggatcctg aatgtgccgt gggccatttt
     9661 ccacatcctg atcattacgc tgcaagcctt catcttcatg gttctgacga tcgtctatct
     9721 gtcgatggcg tctgaagaac attaatttac caacactact acgttttaac tgaaacaaac
     9781 tggagactgt catggaaaac ctgaatatgg atctgctgta catggctgcc gctgtgatga
     9841 tgggtctggc ggcaatcggt gctgcgatcg gtatcggcat cctcgggggt aaattcctgg
     9901 aaggcgcagc gcgtcaacct gatctgattc ctctgctgcg tactcagttc tttatcgtta
     9961 tgggtctggt ggatgctatc ccgatgatcg ctgtaggtct gggtctgtac gtgatgttcg
    10021 ctgtcgcgta gtaagcgttg cttttattta aagagcaata tcagaacgtt aactaaatag
    10081 aggcattgtg ctgtgaatct taacgcaaca atcctcggcc aggccatcgc gtttgtcctg
    10141 ttcgttctgt tctgcatgaa gtacgtatgg ccgccattaa tggcagccat cgaaaaacgt
    10201 caaaaagaaa ttgctgacgg ccttgcttcc gcagaacgag cacataagga ccttgacctt
    10261 gcaaaggcca gcgcgaccga ccagctgaaa aaagcgaaag cggaagccca ggtaatcatc
    10321 gagcaggcga acaaacgccg ctcgcagatt ctggacgaag cgaaagctga ggcagaacag
    10381 gaacgtacta aaatcgtggc ccaggcgcag gcagaaattg aagccgagcg taaacgtgcc
    10441 cgtgaagagc tgcgtaagca agttgctatc ctggctgttg ctggcgccga gaagatcatc
    10501 gaacgttccg tggatgaagc tgctaacagc gacatcgtgg ataaacttgt cgctgaactg
    10561 taaggaggga ggggctgatg tctgaattta ttacggtagc tcgcccctac gccaaagcag
    10621 cttttgactt tgccgtcgaa caccaaagtg tagaacgctg gcaggacatg ctgacgtttg
    10681 ccgccgaggt aaccaaaaac gaacaaatgg cagagcttct ctctggcgcg cttgcgccag
    10741 aaacgctcgc cgagtcgttt atcgcagttt gtggtgagca actggacgaa aacggtcaga
    10801 acctgattcg ggtaatggct gaaaatggtc gtcttaacgc gctcccggat gttctggagc
    10861 agtttattca ccttcgtgcc gtgagtgagg ctaccgctga ggtagacgtc atttccgctg
    10921 ccgcactgag tgaacaacag ctcgcgaaaa tttctgctgc gatggaaaaa cgtctgtcac
    10981 gcaaagttaa gctgaattgc aaaatcgata agtctgtaat ggcaggcgtt atcatccgag
    11041 cgggtgatat ggtcattgat ggcagcgtac gcggtcgtct tgagcgcctt gcagacgtct
    11101 tgcagtctta aggggactgg agcatgcaac tgaattccac cgaaatcagc gaactgatca
    11161 agcagcgcat tgctcagttc aatgttgtga gtgaagctca caacgaaggt actattgttt
    11221 ctgtaagtga cggtgttatc cgcattcacg gcctggccga ttgtatgcag ggtgaaatga
    11281 tctccctgcc gggtaaccgt tacgctatcg cactgaacct cgagcgcgac tctgtaggtg
    11341 cggttgttat gggtccgtac gctgaccttg ccgaaggcat gaaagttaag tgtactggcc
    11401 gtatcctgga agttccggtt ggccgtggcc tgctgggccg tgtggttaac actctgggtg
    11461 caccaatcga cggtaaaggt ccgctggatc acgacggctt ctctgctgta gaagcaatcg
    11521 ctccgggcgt tatcgaacgt cagtccgtag atcagccggt acagaccggt tataaagccg
    11581 ttgactccat gatcccaatc ggtcgtggtc agcgtgaatt gatcatcggt gaccgtcaga
    11641 caggtaaaac cgcactggct atcgatgcca tcatcaacca gcgcgattcc ggtatcaaat
    11701 gtatctatgt cgctatcggc cagaaagcgt ccaccatttc taacgtggta cgtaaactgg
    11761 aagagcacgg cgcactggct aacaccatcg ttgtggtagc aaccgcgtct gaatccgctg
    11821 cactgcaata cctggcaccg tatgccggtt gcgcaatggg cgaatacttc cgtgaccgcg
    11881 gtgaagatgc gctgatcatt tacgatgacc tgtctaaaca ggctgttgct taccgtcaga
    11941 tctccctgct gctccgtcgt ccgccaggac gtgaagcatt cccgggcgac gttttctacc
    12001 tccactctcg tctgctggag cgtgctgcac gtgttaacgc cgaatacgtt gaagccttca
    12061 ccaaaggtga agtgaaaggg aaaaccggtt ctctgaccgc actgccgatt atcgaaactc
    12121 aggcgggtga cgtttctgcg ttcgttccga ccaacgtaat ctccattacc gatggtcaga
    12181 tcttcctgga aaccaacctg ttcaacgccg gtattcgtcc tgcggttaac ccgggtattt
    12241 ccgtatcccg tgttggtggt gcagcacaga ccaagatcat gaaaaaactg tccggtggta
    12301 tccgtaccgc tctggcacag tatcgtgaac tggcagcgtt ctctcagttt gcatccgacc
    12361 ttgacgatgc aacacgtaag cagcttgacc acggtcagaa agtgaccgaa ctgctgaaac
    12421 agaaacagta tgcgccgatg tccgttgcgc agcagtctct ggttctgttc gcagcagaac
    12481 gtggttacct ggcggatgtt gaactgtcga aaattggcag cttcgaagcc gctctgctgg
    12541 cttacgtcga ccgtgatcac gctccgttga tgcaagagat caaccagacc ggtggctaca
    12601 acgacgaaat cgaaggcaag ctgaaaggca tcctcgattc cttcaaagca acccaatcct
    12661 ggtaacgtct ggcggcttgc cttagggcag gccgcaaggc attgaggaga agctcatggc
    12721 cggcgcaaaa gagatacgta gtaagatcgc aagcgtccag aacacgcaaa agatcactaa
    12781 agcgatggag atggtcgccg cttccaaaat gcgtaaatcg caggatcgca tggcggccag
    12841 ccgtccttat gcagaaacca tgcgcaaagt gattggtcac cttgcacacg gtaatctgga
    12901 atataagcac ccttacctgg aagaccgcga cgttaaacgc gtgggctacc tggtggtgtc
    12961 gaccgaccgt ggtttgtgcg gtggtttgaa cattaacctg ttcaaaaaac tgctggcgga
    13021 aatgaagacc tggaccgaca aaggcgttca atgcgacctc gcaatgatcg gctcgaaagg
    13081 cgtgtcgttc ttcaactccg tgggcggcaa tgttgttgcc caggtcaccg gcatggggga
    13141 taacccttcc ctgtccgaac tgatcggtcc ggtaaaagtg atgttgcagg cctacgacga
    13201 aggccgtctg gacaagcttt acattgtcag caacaaattt attaacacca tgtctcaggt
    13261 tccgaccatc agccagctgc tgccgttacc ggcatcagat gatgatgatc tgaaacataa
    13321 atcctgggat tacctgtacg aacccgatcc gaaggcgttg ctggataccc tgctgcgtcg
    13381 ttatgtcgaa tctcaggttt atcagggcgt ggttgaaaac ctggccagcg agcaggccgc
    13441 ccgtatggtg gcgatgaaag ccgcgaccga caatggcggc agcctgatta aagagctgca
    13501 gttggtatac aacaaagctc gtcaggccag cattactcag gaactcaccg agatcgtctc
    13561 gggggccgcc gcggtttaaa caggttattt cgtagaggat ttaagatggc tactggaaag
    13621 attgtccagg taatcggcgc tgtagttgac gtcgaattcc ctcaggatgc cgtaccgcgc
    13681 gtgtacgatg ctcttgaggt gcaaaatggt aatgagcgtc tggtgctgga agttcagcag
    13741 cagctcggcg gcggtatcgt gcgtaccatc gcaatgggtt cctccgacgg tctgcgtcgc
    13801 ggtctggatg taaaagacct cgaacacccg atcgaagtcc cggtaggtaa agcgactctg
    13861 ggccgtatca tgaacgtact gggtgaaccg gtcgacatga aaggcgagat cggtgaagaa
    13921 gagcgttggg cgattcaccg cgcagcacct tcctacgaag agctgtcaaa ctctcaggaa
    13981 ctgctggaaa ccggtatcaa agttatcgac ctgatgtgtc cgttcgctaa gggcggtaaa
    14041 gttggtctgt tcggtggtgc gggtgtaggt aaaaccgtaa acatgatgga gctcattcgt
    14101 aacatcgcga tcgagcactc cggttactct gtgtttgcgg gcgtaggtga acgtactcgt
    14161 gagggtaacg acttctacca cgaaatgacc gactccaacg ttatcgacaa agtatccctg
    14221 gtgtatggcc agatgaacga gccgccggga aaccgtctgc gcgttgctct gaccggtctg
    14281 accatggctg agaaattccg tgacgaaggt cgtgacgttc tgctgttcgt tgacaacatc
    14341 tatcgttaca ccctggccgg tacggaagta tccgcactgc tgggccgtat gccttcagcg
    14401 gtaggttatc agccgaccct ggcggaagag atgggcgttc tgcaggaacg tatcacctcc
    14461 accaaaactg gttctatcac ctccgtacag gcggtatacg tacctgcgga tgacttgact
    14521 gacccgtctc cggcaaccac ctttgcgcac cttgacgcaa ccgtggtact gagccgtcag
    14581 atcgcgtctc tgggtatcta cccggccgtt gacccgctgg actccaccag ccgtcagctg
    14641 gacccgctgg tggttggtca ggaacactac gacaccgcgc gtggcgttca gtccatcctg
    14701 caacgttatc aggaactgaa agacatcatc gccatcctgg gtatggatga actgtctgaa
    14761 gaagacaaac tggtggtagc gcgtgctcgt aagatccagc gcttcctgtc ccagccgttc
    14821 ttcgtggcag aagtattcac cggttctccg ggtaaatacg tctccctgaa agacaccatc
    14881 cgtggcttta aaggcatcat ggaaggcgaa tacgatcacc tgccggagca ggcgttctac
    14941 atggtcggtt ccatcgaaga agctgtggaa aaagccaaaa aactttaacg ccttaatcgg
    15001 agggtgatat ggcaatgact taccacctgg acgtcgtcag cgcagagcaa caaatgttct
    15061 ctggtctggt cgagaaaatc caggtaacgg gtagcgaagg tgaactgggg atctaccctg
    15121 gccacgcacc gctgctcacc gccattaagc ctggtatgat tcgcatcgtg aaacagcacg
    15181 gtcacgaaga gtttatctat ctgtctggcg gcattcttga agtgcagcct ggcaacgtga
    15241 ccgttctggc cgacaccgca attcgcggcc aggatctcga cgaagcgcga gccatggaag
    15301 cgaaacgtaa ggctgaagag cacattagca gctctcacgg cgacgtagat tacgctcagg
    15361 cgtctgcgga actggccaaa gcgatcgcgc agctgcgcgt tatcgagttg accaaaaaag
    15421 cgatgtaaca ccggcttgaa aagcacaaaa gccagtctgg aaacaggctg gcttttttta
    15481 cgcgttgacc cgtcctgaag agcgttcaca tagatcctgc tgatataaaa cacccctgtt
    15541 ttcccgttta ttcattgatc gaaataagag caaaaacatc cacctgacgc ttaaatgaag
    15601 gtactgcctt aattttctgc agacaaaagg cgtgacgatg gtcgaaaatg gcactttcgt
    15661 cagcggggat aatccgttat tgaacaattt atcctctgtc catttcacga tgaaaaaaat
    15721 gtagtttttt caaggtgaag cggtttaaat tcgttctcaa attacagtca ggacgcgtat
    15781 gttgaataat gctatgagcg tagtgatcct tgccgcaggc aaaggcacgc gcatgtattc
    15841 cgatcttccg aaagtgctgc atacccttgc cgggaaagcg atggttcagc atgtcattga
    15901 tgctgcgaat gaattaggcg cagcgcacgt tcacctggtg tacggtcacg gcggcgatct
    15961 gcttaaacag gcgctgaaag acgacaacct taactgggtg cttcaggcag agcagctggg
    16021 tacgggtcat gcaatgcagc aggccgcacc tttctttgcc gatgatgaag acattttaat
    16081 gctctacggc gacgtgccgc tgatctctgt cgaaacactc cagcgcctgc gtgatgctaa
    16141 accccagggt ggcattggtc tgctgacggt gaaactggat gatccgaccg gttatggacg
    16201 tatcacccgt gaaaatggca aagttaccgg cattgttgag cacaaagatg ccaccgacga
    16261 gcagcgtcag attcaggaga tcaacaccgg cattctgatt gccaacggcg cagatatgaa
    16321 acgctggctg gcgaagctga ccaacaataa tgctcagggc gaatactaca tcaccgacat
    16381 tattgcgctg gcgtatcagg aagggcgtga aatcgtcgcc gttcatccgc aacgtttaag
    16441 cgaagtagaa ggtgtgaata accgcctgca actctcccgt ctggagcgcg tttaccagtc
    16501 cgaacaggct gaaaaactgc tgttagcagg cgttatgctg cgcgatccgg cgcgttttga
    16561 tctgcgcggt acgcttactc acgggcgcga tgttgaaatt gatactaacg ttatcatcga
    16621 gggcaacgtg actctcggtc atcgcgtgaa aatcggcacc ggttgcgtga ttaaaaacag
    16681 cgtgattggc gatgattgcg aaatcagtcc gtataccgtt gtggaagacg cgaatctggc
    16741 agcggcctgt actattggcc cgtttgcccg tctgcgtcct ggtgctgagt tgctggaagg
    16801 tgcacacgtt ggtaacttcg ttgagatgaa aaaagcgcgt ctgggtaaag gcacgaaagc
    16861 cgggcatctg acttacctgg gcgatgcgga aattggcgat aacgttaaca tcggcgcggg
    16921 aaccattacc tgcaactacg atggtgcgaa taaatttaag accattatcg gcgacgatgt
    16981 gtttgtcggt tccgacactc agctggtggc cccggtaaca gtaggcaaag gcgcgaccat
    17041 tgctgcgggt acaactgtga cgcgtaatgt cggcgaaaat gcattagcta tcagccgtgt
    17101 gccgcagact cagaaagaag gctggcgtcg tccggtaaag aaaaagtaat tctggccggg
    17161 taatccggtc atctgggatg aggagataac ataatctccc tcccacaagc agtaactata
    17221 aaaataaccc cactctctac aaggctcggg gcgcccgaaa aaacgggcat acaggttgac
    17281 cgacaacgat ataaatcgga atcaaaaact atgtgtggaa ttgttggcgc gatcgcgcaa
    17341 cgtgatgtag cagaaatcct tcttgaaggt ttacgtcgtc tggaataccg cggatatgac
    17401 tctgccggtc tggccgttgt tgatgcggaa ggtcatatga ctcgcctgcg tcgcctcggt
    17461 aaagtccaga tgctggctca ggcagcggaa gaacatcctc tgcatggcgg caccggtatt
    17521 gctcacactc gctgggcgac ccacggtgaa ccttcagaag ctaacgcgca tccgcatgtt
    17581 tctgaacaca ttgtggtggt gcataatggc atcatcgaaa accatgaacc gctgcgtgaa
    17641 gagttaaaag cgcgtggcta taccttcgtt tctgaaaccg acaccgaagt gattgcccat
    17701 ctggtgaact gggagctgaa acaaggcggg actctgcgtg aggctgttct gcgtgctatc
    17761 ccgcagctgc gcggtgcgta cggtacagtg atcatggact cccgtcaccc ggataccctg
    17821 ctggcggcac gttctggtag tccgctggtg attggcctgg gtatgggcga aaactttatc
    17881 gcttctgacc agctggcgct gttgccggtg acccgtcgct ttatcttcct tgaagagggc
    17941 gatattgcgg aaatcactcg ccgttcggta aacatcttcg ataaaactgg cgcggaagta
    18001 aaacgtcagg atatcgaatc caatctgcaa tatgacgcgg gcgataaagg tatttaccgt
    18061 cactacatgc agaaagagat ctacgaacag ccgaacgcga taaaaaacac ccttaccggg
    18121 cgcatcagcc acggtcaggt tgatttaagc gagctgggac cgaacgccga cgaactgctg
    18181 tcgaaggttg agcatattca gatcctcgcc tgtggtactt cttataactc cggtatggtt
    18241 tcccgctact ggtttgaatc gctggcaggt attccgtgcg acgtcgaaat cgcctctgaa
    18301 ttccgctatc gtaaatctgc cgtgcgtcgt aacagcctga tgatcacctt gtcacagtct
    18361 ggcgaaaccg cggataccct ggcgggcctg cgtctgtcga aagagctggg ttaccttggt
    18421 tcgctggcaa tctgtaacgt tccgggttct tctctggtgc gcgaatccga tctggcgctg
    18481 atgaccaacg cgggtacgga aatcggcgtg gcgtccacta aagcattcac cactcagtta
    18541 actgtgctgt tgatgctggt ggcgaagctg tctcgcctga aaggtctgga tgcctccatt
    18601 gaacatgaca ttgtgcatgg tctgcaggcg ctgccgagcc gtattgagca gatgctgtct
    18661 caggataaac gcattgaagc tctggcagaa gatttctctg acaaacatca cgcgctgttc
    18721 ctgggccgtg gcgatcagta cccaatcgcg ctggaaggcg cattgaagct gaaagagatc
    18781 tcttacattc acgctgaagc ctacgctgca ggcgagctga aacacggccc gctggcgctg
    18841 attgacgcgg atatgccggt tatcgtcgtt gcaccgaaca acgaactgtt ggaaaaactg
    18901 aaatccaaca ttgaagaagt tcgcgcgcgt ggcggtcaat tgtatgtctt cgccgatcag
    18961 gatgcgggtt ttgtaagtag cgataacatg cacatcatcg agatgccgca tgtggaagag
    19021 gtgattgcac cgatcttcta caccgttccg ctgcagctgc tggcttacca tgtcgcgctg
    19081 atcaaaggca ccgacgttga ccagccgcgt aacctggcaa aatcggttac ggttgagtaa
    19141 tcactatcat agataaaaaa gggcttgtgg gcggacaata aagtcttaaa ctgaacaaaa
    19201 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca
    19261 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca
    19321 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa
    19381 gactatattc catggctaac agtacaagaa gttccttctt caggtcgttc ccaccgtatt
    19441 tattctcata agacgggacg agtccatcat ttgctatctg acttagagct tgctgttttt
    19501 ctcagtcttg agtgggagag cagcgtgcta gatatacgcg agcagttccc cttattacct
    19561 agtgatacca ggcagattgc aatagatagt ggtattaagc atcctgttat tcgtggtgta
    19621 gatcaggtta tgtctactga ttttttagtg gactgcaaag atggtccttt tgagcagttt
    19681 gctattcaag tcaaacctgc agcagcctta caagacgagc gtaccttaga aaaactagaa
    19741 ctagagcgtc gctattggca gcaaaagcaa attccttggt tcatttttac tgataaagaa
    19801 ataaatcccg tagtaaaaga aaatattgaa tggctttatt cagtgaaaac agaagaagtt
    19861 tctgcggagc ttttagcaca actatcccca ttggcccata tcctgcaaga aaaaggagat
    19921 gaaaacatta tcaatgtctg taagcaggtt gatattgctt atgatttgga gttaggcaaa
    19981 acattgagtg agatacgagc cttaaccgca aatggtttta ttaagttcaa tatttataag
    20041 tctttcaggg caaataagtg tgcagatctc tgtattagcc aagtagtgaa tatggaggag
    20101 ttgcgctatg tggcaaatta atgaggttgt gctatttgat aatgatccgt atcgcatttt
    20161 ggctatagag gatggccaag ttgtctggat gcaaataagc gctgataaag gagttccaca
    20221 agctagggct gagttgttgc taatgcagta tttagatgaa ggccgcttag ttagaactga
    20281 tgacccttat gtacatcttg atttagaaga gccgtctgta gattctgtca gcttccagaa
    20341 gcgcgaggag gattatcgaa aaattcttcc tattattaat agtaaggatc gtttcgaccc
    20401 taaagtcaga agcgaactcg ttgagcatgt ggtccaagaa cataaggtta ctaaggctac
    20461 agtttataag ttgttacgcc gttactggca gcgtggtcaa acgcctaatg cattaattcc
    20521 tgactacaaa aacagcggtg caccagggga aagacgttca gcgacaggaa cagcaaagat
    20581 tggccgagcc agagaatatg gtaagggtga aggaaccaag gtaacgcccg agattgaacg
    20641 cctttttagg ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa agaccaccgt
    20701 tgcctataga cgatttgtgg acttgtttgc tcagtatttt cctcgcattc cccaagagga
    20761 ttacccaaca ctacgtcagt ttcgttattt ttatgatcga gaatacccta aagctcagcg
    20821 cttaaagtct agagttaaag caggggtata taaaaaagac gtacgaccct taagtagtac
    20881 agccacttct caggcgttag gccctgggag tcgttatgag attgatgcca cgattgctga
    20941 tatttattta gtggatcatc atgatcgcca aaaaatcata ggaagaccaa cgctttacat
    21001 tgtgattgat gtgtttagtc ggatgatcac gggcttttat atcggctttg aaaatccgtc
    21061 ttatgtggtg gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg ccatttgtgc
    21121 ccagcatgat attgagatta gtagctcaga ctggccgtgt gtaggtttgc cagatgtgtt
    21181 gctagcggac cgtggcgaat taatgagtca tcaggtcgaa gccttagttt ctagttttaa
    21241 tgtgcgagtg gaaagtgctc cacctagacg tggcgatgct aaaggcatag tggaaagcac
    21301 ttttagaaca ctacaagccg agtttaagtc ctttgcacct ggcattgtag agggcagtcg
    21361 gatcaaaagc catggtgaaa cagactatag gttagatgca tctctgtcgg tatttgagtt
    21421 cacacaaatt attttgcgta cgatcttatt cagaaataac catctggtga tggataaata
    21481 cgatcgagat gctgattttc ctacagattt accgtctatt cctgtccagc tatggcaatg
    21541 gggtatgcag catcgtacag gtagtttaag ggctgtggag caagagcagt tgcgagtagc
    21601 gttactgcct cgccgaaagg tctctatttc ttcatttggc gttaatttgt ggggtttgta
    21661 ttactcgggg tcagagattc tgcgtgaggg ttggttgcag cggagcactg atatagctag
    21721 acctcaacat ttagaagcgg cttatgaccc agtgctggtt gatacgattt atttgtttcc
    21781 gcaagttggc agccgtgtat tttggcgctg taatctgacg gaacgtagtc ggcagtttaa
    21841 aggtctctca ttttgggagg tttgggatat acaagcacaa gaaaaacaca ataaagccaa
    21901 tgcgaagcag gatgagttaa ctaaacgcag ggagcttgag gcgtttattc agcaaaccat
    21961 tcagaaagcg aataagttaa cgcccagtac tactgagccc aaatcaacac gcattaagca
    22021 gattaaaact aataaaaaag aagccgtgac ctcggagcgt aaaaaacgtg cggagcattt
    22081 gaagccaagc tcttcaggtg atgaggctaa agttattcct ttcaacgcag tggaagcgga
    22141 tgatcaagaa gattacagcc tacccacata cgtgcctgaa ttatttcagg atccaccaga
    22201 aaaggatgag tcatgagtgc tacccggatt caagcagttt atcgtgatac gggggtagag
    22261 gcttatcgtg ataatccttt tatcgaggcc ttaccaccat tacaagagtc agtgaatagt
    22321 gctgcatcac tgaaatcctc tttacagctt acttcctctg acttgcaaaa gtcccgtgtt
    22381 atcagagctc ataccatttg tcgtattcca gatgactatt ttcagccatt aggtacgcat
    22441 ttgctactaa gtgagcgtat ttcggtcatg attcgaggtg gctacgtagg cagaaatcct
    22501 aaaacaggag atttacaaaa gcatttacaa aatggttatg agcgtgttca aacgggagag
    22561 ttggagacat ttcgctttga ggaggcacga tctacggcac aaagcttatt gttaattggt
    22621 tgttctggta gtgggaagac gacctctctt catcgtattc tagccacgta tcctcaggtg
    22681 atttaccatc gtgaactcaa tgtagagcag gtggtgtatt tgaaaataga ctgctcgcat
    22741 aatggttcgc taaaagaaat ctgcttgaat tttttcagag cgttggatcg agccttgggc
    22801 tcgaactatg agcgtcgtta tggcttaaaa cgtcatggta tagaaaccat gttggctttg
    22861 atgtcgcaaa tagccaatgc acatgcttta gggttgttgg ttattgatga aattcagcat
    22921 ttaagccgct ctcgttcggg tggatctcaa gagatgctga acttttttgt gacgatggtg
    22981 aatattattg gcgtaccagt gatgttgatt ggtaccccta aagcacgaga gatttttgag
    23041 gctgatttgc ggtctgcacg tagaggggca gggtttggag ctatattctg ggatcctata
    23101 caacaaacgc aacgtggaaa gcccaatcaa gagtggatcg cttttacgga taatctttgg
    23161 caattacagc ttttacaacg caaagatgcg ctgttatcgg atgaggtccg tgatgtgtgg
    23221 tatgagctaa gccaaggagt gatggacatt gtagtaaaac tttttgtact cgctcagctc
    23281 cgtgcgctag ctttaggcaa tgagcgtatt accgctggtt tattgcggca agtgtatcaa
    23341 gatgagttaa agcctgtgca ccccatgcta gaggcattac gctcgggtat cccagaacgc
    23401 attgctcgtt attctgatct agtcgttccc gagattgata aacggttaat ccaacttcag
    23461 ctagatatcg cagcgataca agaacaaaca ccagaagaaa aagcccttca agagttagat
    23521 accgaagatc agcgtcattt atatctgatg ctgaaagagg attacgattc aagcctgtta
    23581 attcccacta ttaaaaaagc gtttagccag aatccaacga tgacaagaca aaagttactg
    23641 cctcttgttt tgcagtggtt gatggaaggc gaaacggtag tgtcagaact agaaaagccc
    23701 tccaagagta aaaaggtttc ggctataaag gtagtcaagc ccagcgactg ggatagcttg
    23761 cctgatacgg atttacgtta tatctattca caacgccaac ctgaaaaaac catgcatgaa
    23821 cggttaaaag ggaaaggggt aatagtggat atggcgagct tatttaaaca agcaggttag
    23881 ccatgagaaa ctttcctgtt ccgtactcga atgagctgat ttatagcact attgcacggg
    23941 caggcgttta tcaagggatt gttagtccta agcagctgtt ggatgaggtg tatggcaacc
    24001 gcaaggtggt cgctacctta ggtctgccct cgcatttagg tgtgatagca agacatctac
    24061 atcaaacagg acgttacgct gttcagcagc ttatttatga gcatacctta ttccctttat
    24121 atgctccgtt tgtaggcaag gagcgccgag acgaagctat tcggttaatg gagtaccaag
    24181 cgcaaggtgc ggtgcattta atgctaggag tcgctgcttc tagagttaag agcgataacc
    24241 gctttagata ctgccctgat tgcgttgctc ttcagctaaa taggtatggg gaagcctttt
    24301 ggcaacgaga ttggtatttg cccgctttgc catattgtcc aaaacacggt gctttagtct
    24361 tctttgatag agctgtagat gatcaccgac atcaattttg ggctttgggt catactgagc
    24421 tgctttcaga ctaccccaaa gactccctat ctcaattaac agcactagct gcttatatag
    24481 cccctctgtt agatgctcca cgagcgcaag agctttc
//
LOCUS       DAJTUN010000055        24009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTUN010000055 DAJTUN010000000
VERSION     DAJTUN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.055"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..431)
                     /locus_tag="OO470_002062"
     CDS             complement(<1..431)
                     /locus_tag="OO470_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6251310.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGT"
     gene            649..1509
                     /gene="tesB"
                     /locus_tag="OO470_002063"
     CDS             649..1509
                     /gene="tesB"
                     /locus_tag="OO470_002063"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6251311.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1558..2844)
                     /gene="amtB"
                     /locus_tag="OO470_002064"
     CDS             complement(1558..2844)
                     /gene="amtB"
                     /locus_tag="OO470_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6251312.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2874..3212)
                     /gene="glnK"
                     /locus_tag="OO470_002065"
     CDS             complement(2874..3212)
                     /gene="glnK"
                     /locus_tag="OO470_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6251313.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3393..5174)
                     /locus_tag="OO470_002066"
     CDS             complement(3393..5174)
                     /locus_tag="OO470_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6251314.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5167..6939)
                     /locus_tag="OO470_002067"
     CDS             complement(5167..6939)
                     /locus_tag="OO470_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6251315.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(6969..7427)
                     /gene="decR"
                     /locus_tag="OO470_002068"
     CDS             complement(6969..7427)
                     /gene="decR"
                     /locus_tag="OO470_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6251316.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7581..8399)
                     /gene="cof"
                     /locus_tag="OO470_002069"
     CDS             complement(7581..8399)
                     /gene="cof"
                     /locus_tag="OO470_002069"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6251317.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8498..10190
                     /locus_tag="OO470_002070"
                     /pseudo
     CDS             8498..10190
                     /locus_tag="OO470_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10255..10950
                     /gene="queC"
                     /locus_tag="OO470_002071"
     CDS             10255..10950
                     /gene="queC"
                     /locus_tag="OO470_002071"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6251318.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11002..11400)
                     /gene="fadM"
                     /locus_tag="OO470_002072"
     CDS             complement(11002..11400)
                     /gene="fadM"
                     /locus_tag="OO470_002072"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6251319.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11494..11865)
                     /locus_tag="OO470_002073"
     CDS             complement(11494..11865)
                     /locus_tag="OO470_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6251320.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12016..13887)
                     /gene="ppiD"
                     /locus_tag="OO470_002074"
     CDS             complement(12016..13887)
                     /gene="ppiD"
                     /locus_tag="OO470_002074"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6251321.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14079..14351)
                     /gene="hupB"
                     /locus_tag="OO470_002075"
     CDS             complement(14079..14351)
                     /gene="hupB"
                     /locus_tag="OO470_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6251322.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14560..16914)
                     /gene="lon"
                     /locus_tag="OO470_002076"
     CDS             complement(14560..16914)
                     /gene="lon"
                     /locus_tag="OO470_002076"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6251323.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17102..18376)
                     /gene="clpX"
                     /locus_tag="OO470_002077"
     CDS             complement(17102..18376)
                     /gene="clpX"
                     /locus_tag="OO470_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6251324.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18502..19125)
                     /gene="clpP"
                     /locus_tag="OO470_002078"
     CDS             complement(18502..19125)
                     /gene="clpP"
                     /locus_tag="OO470_002078"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6251325.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19372..20670)
                     /gene="tig"
                     /locus_tag="OO470_002079"
     CDS             complement(19372..20670)
                     /gene="tig"
                     /locus_tag="OO470_002079"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6251326.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21014..21331)
                     /gene="bolA"
                     /locus_tag="OO470_002080"
     CDS             complement(21014..21331)
                     /gene="bolA"
                     /locus_tag="OO470_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6251327.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21636..22214
                     /locus_tag="OO470_002081"
     CDS             21636..22214
                     /locus_tag="OO470_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6251328.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22258..23733
                     /gene="ampG"
                     /locus_tag="OO470_002082"
     CDS             22258..23733
                     /gene="ampG"
                     /locus_tag="OO470_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6251329.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5631 a         6626 c         5769 g         5983 t
ORIGIN      
        1 gttccgccct ggttgatcac cggctgaacg gtatcggtga taaataccag tttgtcattc
       61 acggtaatcg ccgcactcaa cagaatacgc gcgttcggct gaacatctgc cgggttaaat
      121 ggcagaacaa agctgaatgg tgactgttta ccttcagtac gcaccgcttt ctgcgccagc
      181 acttttgacg gtgcatcggc taacgacgcg tcagaaagtg tcacggtcag cacagcatca
      241 ggcggcagtg cgactttctg acggatccag acggtaccgg agacattcgg ttgctggata
      301 gcaggttgtt gtgttgctga aatagacgta tttgcagccg gggctggcgt ctgaatatcc
      361 gcgcttttat ctgcgcaagc cgccaacgca atcgcaaccg ctaaaccact ggccatgtgc
      421 acgagtttca ttcatttctc cttattatca atgcaccagc gggctaactt tcctcgccgg
      481 aagagtggtt aacaaaatag taacgtcaac aagtgtggca cacatcacgc atttctgcct
      541 gtaattagcc cgtaattcag actatttcac ccatcggacc agtggcaaat ttgcgttata
      601 ctcaactcac tttggcttgc tgcggcagct ttgttactgg agagttatat gagtcaggcg
      661 ctaaaaaatt tactgacatt gttaaatctg gaaaaaattg aggaaggact ctttcgcggc
      721 cagagtgaag atttaggttt acgccaggtg tttggcggcc aggtcgtggg tcaggccttg
      781 tatgctgcaa aagagaccgt cccggaagag cggctggtac attcgtttca cagctacttt
      841 cttcgcccag gcgatagtaa gaaaccgatt atttatgatg tcgaaacgct gcgtgacggt
      901 aacagcttca gcgcccgccg ggttgctgct attcaaaacg gcaaaccgat tttttatatg
      961 acggcctctt tccaggcacc agaagcgggt ttcgaacatc aaaaaacaat gccgtccgcg
     1021 ccagcgcctg atggcctgcc ttcggaaacg caaatcgccc aatcgctggc gcacctgctg
     1081 ccgccagtgc ttaaagataa attcatctgc gatcgtccgc tggaagtccg tccggtggag
     1141 tttcataacc cactgaaagg tcacgtcgca gaaccacatc gtcaggtgtg gatccgcgca
     1201 aatggtagcg tgccggatga cctgcgcgtt catcagtatc tgctcggtta cgcttctgat
     1261 cttaacttcc tgccggtagc tctacagccg cactgcatcg gttttctcga accggggatt
     1321 cagattgcca ccattgacca ttccatgtgg ttccatcgcc cgtttaattt gaatgaatgg
     1381 ctgctgtata gcgtggagag cacctcggcg tccagcgcac gcggctttgt gcgcggtgag
     1441 ttttataccc aagacggcgt gctggttgcc tcgaccgttc aggaaggggt aatgcgtaat
     1501 cacaattaaa aaaatagccg gaggtgaaaa ccgtccggct attttttgca gtgcttgtta
     1561 cgcgttatag gcattctcgc cgtggctgtt gacgtccagc ccttctcgct cttgttcttc
     1621 cggcacacgt aggccaaccg tcagatccgc cagtttgtag ccaataaatg ccaccacacc
     1681 ggaccagacg atcgtaatgg cgatgctttc caactgtacc agcaactgat ggcccatcgt
     1741 cacaccttca gcgaagccca cgccgcccag agaactggcg gcaaaaatcc cggtcatgat
     1801 acagccgaca atgccacaaa cgccgtgcac accgaagaca tcgcagggat catccacccg
     1861 cagcaagcgt ttgagcatgg taacgcccca caaacccgcc agaccagcta ccacgccgat
     1921 aatcaacgcg ccgccaaccc caatgtagcc gcacgctggc gtcacgccga ccagaccggc
     1981 aatcgcgcca gaacacgctc ccagcagaga aggcttacca cgcagcgccc attcaccgaa
     2041 gatccagcca agaattgccg ccgccgttgc gaccacagta ttcacaaatg ccagtgccgc
     2101 gatttcattc gccgtgccca ctgacccggc gttaaagcca aaccaaccga tatagagaat
     2161 ggcagtcccg gtgaagacca tcggcaggtt gtgcggttta aacgcctctt taccgaagcc
     2221 tacgcgtttg cctatcagat acgcgcccac cagaccggcg attgctgcgt taatgtgcac
     2281 caccgtgcca cccgcgaaat ccagcgcacc gtgagaagcc agcaaaccac cgccccacac
     2341 catatgcgca atcggaatgt aagagagcgt cagccatacc accacgaaaa tcaacacagc
     2401 tgagaagcgg attcgttccg ccagcgcccc aactatcaag cctacggtaa tgcaggcaaa
     2461 cgatccctga aacgctacgt ggatatactg ataaatgctg cccatcaccg ccgtcagttc
     2521 gatgtttttt agcatcaacc agttaatgtt gccgaagaag ttgttgccct caccaaacgc
     2581 cagcgaataa ccgtaaacca cccagagaat acagaccagt gcaaacgtca ccgtcacctg
     2641 cgtcagcatc gacagcacgt ttttgccgcg aatcaaccca ccgtaaaaca gggcaatccc
     2701 cggaatagtc ataaacagca ccagcgcagt acaaatcatc ataaacgcat tgtcggcttt
     2761 atcggccacc gcaggtgcag ccattaccag tcccggcagc atcgccagtg aagcaagccc
     2821 agtttttatc gtcgctatct tcatttttcg ttcctgttgc tgtgtgccag agattacagc
     2881 gccgcttcgt cggcttcgcc ggtacgaata cgaatgacgc gttgcaattc agcgacgaag
     2941 attttgccgt cgccaatttt tccggtgtaa gccgccttac tgacgatatc gatcacttca
     3001 tcgagttggt catcagcaat cgccacatca atttttactt ttggcaggaa gttgacgctg
     3061 tattccgccc cccggtacag ctcggcatgc cctttctgac gcccgaaacc tttcacttcg
     3121 gtgacggtca ggccctgaat accaatggaa gataacgctt cacgaacgtc ttccagcttg
     3181 aatggtttga ttatcacggt caccagcttc atagatcccc tccggtcaga attcggtaat
     3241 ggttctgcta cacgaagaag gtattgcaag cggtgtgcca gaaatgaaaa acgagaaaga
     3301 gagcaggaag ttaagaagat tgaaaatgaa aacgcactat gacagtgcac agtgcgttac
     3361 atttttgcac caacgatgaa atattgcgct attcaggcgc tcaatgattc ctcttcacgc
     3421 acgctggctg ccagctcttc gcccgcaagt tgcagttgat acatctgcca gtagcgtccc
     3481 tgggccgcca gcagttgctg gtgagtgccc tgctccacgg cttgcccacg atgaagcacc
     3541 agaatggtgt cggcatcaat aatagttgat aagcggtgag caatcaccac cagcgtggta
     3601 tgttcacgca ccgccgccag agcatgttga atcgcctgtt cagtaccgga gtcaatgctg
     3661 gcggttgcct catcaaggat caggatttgc ggcgtctcca ccagcacgcg cgccagtgcc
     3721 agcagttgct tttgcccgac tgagagatta tttccctgct cgcccagcgg cgtataaata
     3781 caatcactca tgctgcgcgc cagctccgcc agttgcacgg tttccagcgc ctgccagacg
     3841 cgttcttcgg agatatcccg ccccagcgtc acattagcga ggaaggtatc cgccagcacc
     3901 accggatctt gctgcaccat tgccacgccc tggcgcagcg cgctatgact tagcgaactt
     3961 aatggacgac catcaaggtg aatctcacct tccgttagcg ggtaatagcc catcaataaa
     4021 ctggcgaggg tacttttacc actgccggta tgcccgacca gcgccacaaa gttacgcgaa
     4081 ggcacagaga gattaatgtt ctttagcacc agattgtcat cgcgataagc aaatgacacg
     4141 ttatcgactt cgatggtgcc actctgtaac gggtaatcat cattgccata ttgctggcgc
     4201 ggtccgtcca tcagttcaaa cacgcgctca ccagcaacaa cagcctgttg cagcatcgcc
     4261 tgttgcgtgg tcagttcgat taagggttcg ttaagtcgcc caagatagct gataaacgca
     4321 tacaacacgc ccacttcaat ggtgccgctg gcggagaagc caaacagcat caataagcca
     4381 caaagaatga gcgatgaaaa cagactcagc agcggacgca gcagaaaacc gtcgaggcgc
     4441 agggtttgca tcctagccat atagtgtgaa cgactggcct cccccatacg ttcgccaaat
     4501 cgcgcctgct gacgaaactg ctggataacg ctcatgccat tgatgatttc gttaaagccg
     4561 tcgttgatat ccgccagata ggcgcgcaca cggcggacaa tcggcgtgct gtaacgctgg
     4621 tatatcacca ttaccaccag caccaccggg aaaatcatta tcgccaccag cgccattcgc
     4681 cagtcgaggc tgaacatcgc caccagcatc gcgcccacca gtgcggcact gcgcaggaca
     4741 gttgccacta cggtaacgta gagatcgcgg atcacttcag tgtcattagt gacgcgggaa
     4801 atcacctgcc cgacgggttg ggtatcaaac tcgcttaatg gctggcgtaa cgcagcatcc
     4861 atcacgtcgg tacgcaactg ttgcactgcg cctactgccg cccgattaaa cagcagcgac
     4921 tgcgcgtaat gtagcccggc ggcaaacagt tgtagcccaa catacgccgc agccagccct
     4981 gcaaccactt tcaacggcag gttatttttc gctaccatat tgtcgataaa atagctgata
     5041 agcagcggcc cactgacttc tgccgccgcc gcaacccaca tcatcaggac cgcaatcccc
     5101 agcggtttac gccacggcga accatacgct aacaggcgct tgagagtcgg ccacagttgg
     5161 ctaaaactac gcatcgacgg cctcctcgcg aatttccgga acgtcgtcga gcgccgcctc
     5221 cagttgttga tagcgataca tatcgcgata ccaaccgctt tgttgcgcca gctcatcatg
     5281 attgccacgc tgggcgatat gtccgtgctg catcacaata atttcactgg cttccgtcag
     5341 tgccgaaagg cgatgggcac tgatgattac cgttcttccc tgtccccact gacgcaggtt
     5401 atgcaggatc tggtgctctg tgcgtccgtc caccgccgaa agcgcatcat caaggatgag
     5461 gatttccgcg ttgactaata acgctcgagc aatggagata cgctgttttt gcccaccgga
     5521 aagcatcacg ccgcgctcgc ccacttctgt atcgtaacct tgcggtagac gcaaaatatc
     5581 gtcatgtacg ctggctaacc gcgcgacatg ctcaatctct tgctgggtgg cattcgggca
     5641 acccagcgcg atgttattcg ccacggtgtc agaaaaaagg aatggcgtct ggctgaccac
     5701 cgccaggcgg ctacgccagc tatcgagttg taacttcgtc agaggaatat catgaaagcg
     5761 aatatccccc tcgctgacgt cgaaatgacg ctgaatgagc gacaacaggg tacttttgcc
     5821 ggaaccagtc ggcccgcaaa tacccagcat ctgaccgggt ttcagggcga aattgacgtt
     5881 ttccagcgca ggatggtcag tctgcggata cgtgaactgg tgaatattta catccagttc
     5941 gccacgccct tccggcacgg gttcgctacc atcgttcacc accggcgctt ccgccagcat
     6001 cgcgcgaata cggctgtatg cagcactacc acgttccaca atgttaaaca tccatgccag
     6061 cgccagcatt ggccaaatca tcagacctaa atacatcata aaactggtga gctggcccag
     6121 cgttaaactg ccctgcacca ccatccaact accgccgcca atcgccagca agttcgccat
     6181 accaatcgcg atatagatgg tcgggtcgaa gcgagcatca atacgcgcca cccgcatgtt
     6241 ttttttgccg gtatcttcgg catccgcggc aaataacgcc gactggcgat cttccagacc
     6301 aaaggctttg atcatgcgga tactggtgag gctttcctgg gtgcggtcat taagactgga
     6361 aaacgccgcc tgtgccagct taaagcgttc atgcaaggca tcgccgttgc gcttaatcat
     6421 gatcgccatc actggcatcg gtaacagggc aaataaggtc aactgccagc taatttgcgt
     6481 agacatcata atcaacacag cgcagcccat taccagtgaa tccaccagcg tcagcacccc
     6541 ttctccggcg gcaaacacga cgcgatcgac gtcattggtc gcacgagcca tgagatcacc
     6601 cgtgcgatga cgcaggtaaa actccgggtg ctgccggctt agctgacggt aataatcttc
     6661 acgcagttca acagccagtt ggtaagacgc gccaaacagc aatacgcgcc agacataacg
     6721 caggagataa accacaacgg caatcagcac catggtggcg atccacatca ggctcagccc
     6781 ggtagtaaag tgttgttctg tcacgccatc gacaacaata ccaaccactt ttggcggaac
     6841 cagttgcagc atcgcgataa tgacaagcaa ggcgacagcc ccaagatagc gacgccattc
     6901 ccgacggaaa taccagctta attgagcaaa taatcgcacg cgttgtgtcc tgacctgatt
     6961 ctggatattt attcgatggg taaagaagtg gtgtatttaa tctgttccat cgcgaagctg
     7021 gaagtgacgt ccgacagccc cggcacgctg tttaccagac gcttataaaa ctcgtcgtag
     7081 cgtttcatgt cggcaacctg gacgcgcatc agataatcgt attcaccagc catgcgccag
     7141 acccccagca cttctggcat ttcggtaacc accgtgacaa agcggcaata ccattcgctg
     7201 ctgtgatgtt gcgtttttat cagcacaaag gcggtcaggc cgaggcctat tttttccgga
     7261 tccagcaggg cgactttgcc gataaggata ccgtcgtcct ccagccgttt caggcgcttc
     7321 cagcaggggg tggttgtcag attaacggct tcagccagtg cctgcaaaga gagggtgcaa
     7381 tcctgctgca gtaaggccag cagcttacgg tcaattttat ctaacatagc ccttccacgg
     7441 agaatttttt tctcttttaa ttctatttta aaggtgaaat cgcgacaatt tattctgtgc
     7501 ggtttaaaca ccacggcaca aaatgacaaa ttcaacgcaa aaaaaaaccg ggcaattgcc
     7561 cggtgctgga agggatctcg ttattcgggg gaataaggta gatgtggata gtccagccag
     7621 tgcgtcaaat agtgagagac agcctgattt cggcaatgtc caatcaccgg taaatgcggg
     7681 agctccgcgc gcagttgcgg catcgcattg cccataataa acccgctacc gacgctgcct
     7741 aacatttcgc gatcgttcat cgcatcacca aaggccatgc aatcgcgcaa cgataaacct
     7801 aaatgttggg tccgcaccgt caatgcagcg cctttattgc agcccaccgg cagcacttcg
     7861 aggcaatccg tggcggaaaa acacaaatgt gcacgctcgc ctaatgcttc gtatagctgg
     7921 atctgcaagc gtgtaagatc gtcgtgatcg ccacagaagc agatcttggt gacgctgccg
     7981 agtggcattt ttttgacatc gattatctga taacgaaaac cgctatagac aaatacctgc
     8041 aacaacgcag ggctctcttt cccggtaaac caaccgtcgt cattgaagat atgcatgctg
     8101 gctcgggtat cccattgctg atacagcacc agctccgcga catccgcagg taaatcatca
     8161 cgatgtaaaa gttcaccttc cagagaatgc acgcgcgttc cgttgccggt aatcaaatat
     8221 gcatccagcg atagcgcccc gagaatatgc tgcatctcca gcgcatgacg ccccgtggca
     8281 aaagtgaggg taatgtcgca ttcacgcagt cgcgccaaag tagagagggt tttctcacct
     8341 aaatgatggt caggcatcaa taaagtgcca tccatatcaa atgctaccag acgagccatt
     8401 tctttctcca ctctgtgaca cggttaatgg tgattgagta tcacctgata tatacggaag
     8461 taatagtgaa tagttaataa tattgttccg ggtttatatg cgattgctca accgtcttaa
     8521 ccagtatcaa cgtctgtggc aaccttcagc cgcaaaccgt caccgtcagc gaactggccg
     8581 aacgctgttt ttgcagcgaa cgccatgttc gtacgctgtt gcgtcaggca caggaggcgg
     8641 gatggctgga gtggcaggcg cagtcaggac gcggaaagcg cggacaatta cgctttctgg
     8701 tcacgccgga atcgctacgc aatgcgatga tggaacaggc actggaaacc ggaaagcagc
     8761 aagatgtgct ggagctggcg caactggccc caggtgagct gcgcactctg ttacagccat
     8821 ttatgggcgg acaatggcaa aacgatacac ccacgttgcg tattccctac tatcgcccgc
     8881 tcgaaccgct acaaccaggc tttttgcccg gccgtgccga gcagcatctc gccgggcaga
     8941 tattttccgg cctgacccgc ttcgataata atactcagcg cccgattggc gatttagcgc
     9001 atcactggga aacctctact gacaggttac gctgggactt ttatcttcgt tcaaccctac
     9061 actggcataa cggcgatgca gtaaaagcct cacacttaca ccagcgatta ttgatgctgt
     9121 tacaactgcc agcactggat caattattta ttagcgtgaa gcgtattgaa gtcacccatc
     9181 cgcagtgtct gaccttcttt ttacatcgcc ccgattactg gcttgcgcac cggctggcga
     9241 gctattgcag ccatctggcg catccgcaat tcccactgat cggcactggt ccttttcgct
     9301 taacacaatt cacagcggag ctggtgcgcc tggagagcca tgattattac catttgcgtc
     9361 atccgctgct taaagcggtt gagtactgga taactccgcc gcttttcgaa aaagatttgg
     9421 gaaccagttg tcggcatccc gtgcaaatca ccatcggcaa accggaggag ctgcaacggg
     9481 tcagccaagt cagtagtggc atcagtttag gtttttgcta tttaacgttg cgcaaaagtc
     9541 cccgactctc cctctggcag gcgcgaaaag tgatctccat tattcatcaa tccggtttat
     9601 tacaaacgtt agaagtcgga gaaaacctga tcaccgccag tcatgcatta ctgctaggct
     9661 ggactattcc gcactggcaa gtaccggatg aagtcaaact accgaaaacc ttgacgctgg
     9721 tttatcacct accgatagaa cttcatacca tggcagaacg cctacaggcg acactggcag
     9781 cggaaggctg tgaactcaca attatttttc ataacgcaaa aaactgggac gacacgaccc
     9841 tactggcaca cgcagacctc atgatgggcg acagattaat tggcgaagca ccggaatata
     9901 ctctggagca atggctacgt tgcgatccac tatggccaca tgttttcgac gctccagcat
     9961 atgcacatct gcaatcgacg ctggacgcgg tgcaagtaat gcctgatgag gaaaaccgat
    10021 ttaatgccct gaaagcggtt tttagccagt taatggcaga cgcgacgctg acgccgctgt
    10081 tcaactatca ctatcgcatt agtgcccctc ccggcgtgaa cggtgtgcga ctgacaccgc
    10141 gcggctggtt tgaatttacc gaagcctggc ttcccgcgcc gtcgcaatga atagctggtc
    10201 cgggttaatc gcaggcgtta ccataacgct tttattgttc aagcaggatt atctatgaaa
    10261 cgtgctgtcg ttgtgttcag tggaggccag gattccacca cctgtctggt gcaggcatta
    10321 caacaatatg atgaagtcca ttgcgtgacg ttcgattacg gtcagcgaca tcgcgcagaa
    10381 atcgacgtgg cacgcgaact ggcgctgaaa ctgggagcac gcgcgcataa ggttcttgat
    10441 gtaacgctgc tcaacgagct ggcggtcagc agcctgacac gtgacagcat tccggtgcct
    10501 gattatgaac ctgaagccga tggcatcccg aatacgtttg tcccagggcg taatattttg
    10561 ttcctgacgc tggcggcaat atatgcgtat caggtaaaag cagaagccgt aattaccggc
    10621 gtctgtgaaa cggatttctc cggctacccg gattgccgcg atgagtttgt gaaagcacta
    10681 aaccatgccg tcagtttggg catggcgaaa gatattcgtt ttgaaacgcc gctgatgtgg
    10741 attgataaag cggaaacctg ggcgctggca gattattacg gcaaactgga tttagtccgt
    10801 aacgaaacgt tgacctgcta taacggcatt aaaggcgacg gttgcggtca ttgtgcggca
    10861 tgtaatttac gcgccaacgg tttgaatcat tatctggccg ataaaccgac ggtgatggca
    10921 gcgatgaagc agaaaaccgg gttgaggtaa ttattccggg tgtcgccgga tgcggcttga
    10981 gcatccggca ccacaaaacg tttacttaac catctgctcc agcttttcgc gcaattcccc
    11041 ttccagagct aatgctttct gcgttttaag atcaatacaa acaaacgtaa taagcgcatc
    11101 cgctaccacc tgcccttccg gctccagtgt aatgacctgg tttaagatgc cgcttttacc
    11161 gtttaattgc tgcaactgac tggtaatagt taacaggtcg cttaataccg ctgggcgacg
    11221 atagttaata ttgatattga ccacgacgaa ggcgatgtta tgggccgtca tccactgaaa
    11281 actgtcgcta ttttccaccc catcccagcg ggcttcttcg agaaattcaa ggtagcgggc
    11341 gttgttgacg tgctggtaaa cgtcgagatg atatccacga actttgattt gtgtttgcat
    11401 agcgcaataa ccttacgttg ttgttataag tacagaggtc ataactggta tgacctcttc
    11461 agtctggcaa aaaattgcca ctatgcaaat taattacagg gttaataccg ccagattacg
    11521 ttccaccagc gaattgccca tccccggcac ctgctttaga tcctccaccg ttttaaacgg
    11581 accgtactct tcgcgataac tgacaatcgc ctgcgctttc ttcaggccaa cgccattcat
    11641 cgcgcgggct agctcttccg cgctggcatt attaatgctg acccgggtgc cttcttcgtc
    11701 actggctttc gccggtactg ctgctttact ttgtgctgca ggagcttccg ctttggtttc
    11761 taccgccgtc ggtttcgcca cagaagctgc cgccagcgcg ctatgagaca ttccggcaca
    11821 ggccagggac agggtaatga gcagtgcttt aattccgtgt ttcatactgt tttctccttg
    11881 tttgttaaca gtggaatcac cttaacggga gtgaaaacag taaacaaatg gcaatgttca
    11941 gaaatggaaa aggccgcgaa agcgaccttt tcttgttaca gctgcaacgc atttttttgc
    12001 gaggcggctt caggattatt gctgttccag cgcatcgcca attttgattt tcgcctcttt
    12061 acgcaggtta ctcatcagag cttcaaagac gatttgtgcg ttgttctggg tgataccctg
    12121 caccatcgct tttttctgat cttccggcat tgaaccttgt ttcacttcat ccagcgccag
    12181 cagaaccaca ttaccttgca tatcggtcgc cataccgtag ctcggtttgt ctttcgctgg
    12241 cagtggcagt gcaaacgccg cctggctaat cgggtcacga ccggaacggc ttaaggtttt
    12301 cggctcgcca aatttcagac cggcagcctg catagcttcc gcacctttgc cggctttcaa
    12361 atcaaccagc agtttctcag catccacttt cgcctgttgt tcagctttgt tgtgctgaac
    12421 cagtgcctta acttgttcct gaacatctgc caacggtttc accgcttccg gtttgtgctc
    12481 gctgacgcgc agcacgaatg cgcggtcgcc gtctacggtg atgatgtcag agttgatgcc
    12541 cgacgcgccg ttttcaccta ccagaccgcc gttaaagata gcgtcggcaa ccggcttgaa
    12601 gttcaactct tccggcaggt tatctttgct gaaccaaccc gtctgagtgg ctttaacgcc
    12661 ggcagcttgc tctgcaccgg ccagagactc ggtgtcgttg cttgccgcat cgctcacttt
    12721 ctgctgcagc gcatagtacg catcgagggc tttttcgtgt ttcactttcg ccgcaatgtc
    12781 gtcacgtact tcgtctaacg atttcacttt cgctggctga atgtcgtcca gacgtacaat
    12841 caggaaaccg accgaagatt tgatgacacc agacagttgg cctttttctt tcagaccagc
    12901 atttttcagt tcgtcgggga tagtggcatc ttctaaccaa cccatatcgc cgccgttacg
    12961 ggcagagata atatcggcag atttttcttt ggctaatgca gcaaaatcac cgcctttatt
    13021 cagctcatca agtaccgctt tcgcttcatc ttcagttttg gtctggatga tgctgtagcg
    13081 ggtacgctgc ggctgggtga attgatcctg atgctgatcg tagtagctct ggatatccgc
    13141 atcgctaacc ggttgctgca tcgttgcggc aaccagcttg atgtaactca cgcggaattg
    13201 ttccggcgtc atgaaattgt ttttgttttg ttcgtagtag ctggcaatct cctgttcggt
    13261 cacaggctgc ttcgccgcca gcgcgttaac atcgatagtc gcctcacgca ccacgcgttg
    13321 ttgcgcgacc agtgccgcca gctcgtcggt ttcacctttc agcataaaat cggtaccggc
    13381 aacgccgtta atcagctgtt gggtagtgag ctggttacgc agcgcctggg cgtactgatc
    13441 ggcggtcatc cccatctggt tgaggatacc gttatagcgg ctgttatcaa atttgccgtc
    13501 aacctggaag gctggggtcg cgaaaatcgc ctgtttaacc tgctcatcac tgatacccag
    13561 tttcagctca cgagcgtact gatccaacag cgcctcgtcg atcagacgat tcagcacctg
    13621 ttgacgcagg gttttcatat agccttcgtt cgctgccagc tcggagtatt gatcgcccag
    13681 ctgttgctgc atacgattac gctcgctgtt aaaggcgttc tcgaactgcc cacggctgat
    13741 ttcctggtca ttcacttttg cggcgtagtt attgcctccg ccaatcaggt aaccactcac
    13801 gccggtcaat atgaacgaca cgataatgat accgaaaata atcttgagca cgagactgtt
    13861 tgcagccgtg cgtaagctgt ccatcatggt gtaacaacac tccgctgtag atgactgtgt
    13921 acctcacgca acgtatcgat gcgcgcaaag gcctattgtg acaagaaacg ggggcaattg
    13981 tcagcccaca agacacagaa agtcaccgaa tacaaataaa aaaggcacat cagtagatgc
    14041 gcccttgaac ttcgtcacat ccccactggg gacaacgctt agtttaccgc gtctttcagt
    14101 gctttacctg cacggaagct cggtacttta gcggcagcga tggtgatctc tttaccggtc
    14161 tgcgggttgc ggccagtacg ggcagcacgc tctttaacgg caaaagtacc aaaacctacc
    14221 agtgctacat catccccttc tttcagagat tcagttacgg aagcaataat agcatctaac
    14281 gcacggccag ccgcagcttt agagatatca gcccctgcag caatcttgtc gatcaattga
    14341 gatttattca ctcttctctt cctctttata atttatatcg cacttgaatc cttcaaggta
    14401 cgaacgcgca gcagttatat caggccagcc atccccttac aacacccctt aataagggca
    14461 agcccgatcc gctatctaac ttagcgagac aaaaaaaggc tggcaagccc gaattagcct
    14521 gccagccctg tttttattag tgcattttgc gcgaggtcac tattttgcag tcacaacctg
    14581 catgccagac ggttcatttt gcagcgccag agtcagaact tcctcaatgc gcttcacagg
    14641 atgaatgtcc agatcggcaa ttacgttgtc aggaatctct tccagatcgc gtttattttc
    14701 gaacggaatt agcactgttt taatcccgcc gcgatgcgct gccaggagtt tttctttcaa
    14761 accaccgatc ggcagtacct gaccacgcag agtgatctca ccggtcattg ccacatcggc
    14821 acgaaccggg ttaccggtca ggcaagaaac cagcgcggtg cacatagcaa taccggcact
    14881 cggaccatct ttcggcgtcg caccttccgg tacgtggacg tggatgtcac gtttttcgta
    14941 aaaatcaggg ttgatcccca gtttttccgc acgcgcacga accaccgtta acgccgcctg
    15001 aatagactcc tgcatcactt cgccgagcga accggtatag gtcagtttgc ctttacccgg
    15061 aacacatgcg gtttcaatgg tcagcaagtc accgcccact tccgtccacg ccagaccgat
    15121 tacctgaccg acacggtttt cgttatcagc gcgaccatag tcgaaacgct gaacaccgag
    15181 gtagtcatgc aggttatcgc cgttaatttc gatatgtttt aatgacttat cgagcagtaa
    15241 ctgcttaacc gctttgcggc acagtttgga gatttcacgc tccagaccac gcacgcccgc
    15301 ctcacgggtg tagtaacgaa taatgccgat aatggcgcta tcgtcgacgg tcagctcacc
    15361 ttttttcagt gcattacgtt caatctgctt cggcagcagg tgacgtttgg cgatgttcag
    15421 tttttcatct tcggtataac cggagaggcg aatcacttcc atacgatcca gcagcggtgc
    15481 cggaatgttc atggagttcg acgtcgcgac aaacatcacg tcgctgagat cgtaatccac
    15541 ttccaggtag tggtcgctga acgctacgtt ctgctctgga tccagcactt caagcagtgc
    15601 agaggccgga tcgcctcgca tgtcagaaga cattttgtcg atctcatcga gcaggaacag
    15661 cggatttttc acgcccactt tcgccatttt ctggatcagt ttacccggca tagaaccgat
    15721 gtaagtacgg cggtgaccac ggatttccgc ttcatcacgc acgccgccca gcgccatacg
    15781 gacatattta cgcccggtgg ctttggcaat ggactgacca agagaggttt tacctacccc
    15841 cggcggccct accaggcaga ggatcggtcc cttgattttg ttgacacggc tttgaaccgc
    15901 aaggtactca aggatgcgat ctttcacgcg ctccagacca taatggtcgg tatcaaggat
    15961 ttcctgcgcc tgacgcaggt cttttttgac cttgctgcgc gcattccacg gtacctgtac
    16021 catccagtcg atataaccac gcactacggt cgcttctgcc gacatcggag acatcatttt
    16081 cagcttctgc aactctgctt ccgctttctc ttttgcctct ttcggcatct tcgccgcgtc
    16141 gattttgcgc ttcagggctt cgttttcgtc cggcacgtcg tccatttcac cgagttcttt
    16201 ctgaatagct ttcatttgct cgttcagata gtactcacgc tgggatttct ccatctgctt
    16261 tttaacgcgg ttgcgaatgc gtttctcaac ctgcagcaga tcgatttccg attccatcat
    16321 tgccatcaga tattccagac gttcgttaac gtcggacatc tccagaacgg actgtttgtc
    16381 agccagtttc agcggcatat gtgcagcaat ggtatccgcc agacgcgccg gatcgtcgat
    16441 gctattcagc gacgtcagca cttctggtgg gatttttttg ttcagcttga tgtagccttc
    16501 gaactggctg attgcagtac gcaccagcac ttcctgttcc cgctcatcaa tggtcggcga
    16561 ctccagatac tccgccttcg cagaaaagtg ttcgccattg tcagagagcg cagaaatacg
    16621 cgcgcgctgt aacccctcga ccagcacttt gacggtgccg tcaggcagtt tcagcatctg
    16681 caatatagag gccacggtcc cgacggtgaa aagatcgttt acacccggct catccgttga
    16741 agcttctttc tgcgcgacca gcataatttt tttatcatgg tccatcgccg cttccagaca
    16801 acggatagat ttttcccgcc cgacaaataa ggggatgacc atgtgcggat aaaccaccac
    16861 atcgcgcagc ggcaatacgg ggatttcaat gcgttcagaa cgctcaggat tcatagagct
    16921 ctctcttagt ttaatttccg ccaggtaatc agatgacacg actgtgcttc acgccatcta
    16981 ttaacatgta cgtcagtata tggggatgtt tcccccacat tcaacgccga gaatagagga
    17041 aaaattaaag gggagataaa atcccccctt tttggttaac taattgtatg agaatggtta
    17101 attattcacc agatgcctgt tgcgcttccg gcttaccata aatcagcaac ggtttgcttt
    17161 gaccatcaat taccgactcg tcgataacca ctttttcgac gtcttccatg gacggcagat
    17221 cgtacatggt atcgagcagt gcggcttcta cgatggaacg caggccacgg gcaccggttt
    17281 tacgcgccat cgctttctta gcgatagcat ccagcgcctc gtcacggaat tccagatcca
    17341 cgccttccag attaaacagc gcctgatact gcttggtcag ggcgtttttc ggctctttga
    17401 ggatctgaat cagagcttct tcgctcagtt cattcaacgt tgctacaacc ggcagacgac
    17461 caatgaactc agggataaga ccaaacttga tcagatcttc cggttcaacc tgcgccagca
    17521 gctcgccttc gcttgctttg tcggacttcg cttttaccgt cgcgccaaaa ccaatgccgg
    17581 agccggtttc tacacggtgg gaaatcactt tatccagacc ggcaaacgca ccgccacaaa
    17641 taaacaggat cttagaggta tcaacctgca agaattcctg ctgcggatgt ttacgcccac
    17701 cttgcggtgg aacagcagct accgtacctt cgatcagttt caacagtgcc tgctgtactc
    17761 cttcaccgga aacgtctcgg gtaatggacg ggttgtctga cttacgagaa atcttgtcga
    17821 tttcatcgat gtagacaatc ccgcgctgcg ctttctgtac gtcgtaatcg cacttttgca
    17881 acagcttctg aatgatgttt tcaacgtctt cgcccacata accggcttcg gtcagcgtgg
    17941 ttgcgtcggc catggtgaac gggacgtcca gcaggcgcgc cagcgtttca gccagcagcg
    18001 ttttaccgga accggtcgga ccgatcagca gaatgttact tttgcccaac tcgacaccat
    18061 tgctggtatc gccgttgcgc agacgtttgt aatggttgta taccgcgacc gccagcactt
    18121 ttttcgcctg ttcctggccg ataacgtaat cgtccaggtg gttgcgaatt tcatgcggcg
    18181 tcggtagcgc actgcgttca cgatgcggtg caacttcttt aatctcttcg cgaatgatgt
    18241 cgttacataa atcaacacat tcgtcgcaga tatacacgga tggaccggca atcagcttgc
    18301 gcacttcatg ctggcttttg ccgcaaaaag agcaatacag caatttgcct gagccatctt
    18361 tgcgtttatc tgtcatgggt caaaacctct tctttgttct ttgtgccgca cacgacgacg
    18421 caaatgccat tctcaggcgc aagccgctta cagcgttgtg ccgccctaga taagtatagc
    18481 ggcacagttg cgcctctggc atcaattacg atgggtcaga atcgaatcga ccagaccgta
    18541 ttccaccgct tcaggggcgg aaaggaagcg atcgcgctcg gtatcacgtt caatctgttc
    18601 taatgattga cccgtatgaa gcgccataag ttcattcatg cgccctttaa ctttcagaat
    18661 ttcacgggca tgaatttcga tatcggtcgc ctggccctgg tagccgccca acggttggtg
    18721 aatcatcacg cgcgaattcg gcaggcaaaa acgtttacct tttgcccctg cggtcaacaa
    18781 gaaagcgccc atcgaggccg cctggcccat acagatggtg ctgacatcag gcttgataaa
    18841 ctgcatggtg tcatagatag acatcccggc agtgatcacc ccgcctgggg agttaatgta
    18901 cagatagata tctttttctg ggttttccgc ttccaggaac agcatctgcg ccacaatcag
    18961 gttagccatg tggtcttcaa cctggccagt cagaaaaatg acgcgttcct taagtagacg
    19021 agaatagata tcaaaagagc gctcaccgcg tgaggtctgt tcaatgacca tcggcaccag
    19081 cgccatatgg ggtgcaaagt tatctcgttc gccgctgtat gacatttccg tctcctggat
    19141 aaaattgaaa aaaacctgct gtaccgattg taaccttatg gacggattat cagctagtcc
    19201 ctgtgttatg ggtacggcat caccatattt caagcataac aatcttttgt tgttacgcta
    19261 acaccgaaag aggcttttcg cacggctcca tgcaaaattc atgacaaaaa aaagcccacc
    19321 acctggcggt gacgggcctt tgtgcgaatt tagcgcgtta tgctgcgtaa attacgcctg
    19381 ctggttcatc agctcgttga aagtggtttc tttttcagtc actttcgctt tcgccagtac
    19441 agcttcaaca gcctgttctt ccagagcaac attgcgcatg ttgtccatca gttctttgtt
    19501 tttgctgtag aactcgataa cttctttcgg atcttcgtac gcagaagcca tctcttcgat
    19561 caggcctttc acgcgctctt cgtcagcttt cagctcgttg gtgcggataa cttcgcccag
    19621 cagcaggcca acaactacgc ggcgtttagc ctgttcttcg aacagttcgc gcggcagttc
    19681 cagagcttgt ttttcgttgc caccgaaacg ctgtgcagcc tggcgacgca gaacgtcgat
    19741 ttcgctgtcg atcagcgcag ccggtacgtc gatgtcgtta gcttttacca gaccttcgat
    19801 cgcctgagac ttaacgcggt tacggatggc gctcttcagc tcgcgctcca tgtttttacg
    19861 cacttcagcg cgcagacctt ctacggaacc atcttcaacg ccgaaacgtt tgatgaattc
    19921 tgcagtcagt tccggcagtt cacgctcttc aactttcttc aggttgatag cgaatttcgc
    19981 tgctttacct ttcaggtttt ctgcgtggta ttcttccggg aaggtcacgt cgatggtgaa
    20041 ctcttcgcca gctttgtggc ctttgatacc gtcttcaaag cccgggatca tacgaccctg
    20101 gcccatcgcc agtacgaaat cagacgcttt accgccttcg aactcttcgc cgtctacaga
    20161 accggtgaag tcgatggtta cgcggtcttc tgcttcaaca gcgccgtctt tttctttcca
    20221 ggtcgcctgc tgtttacgca gagtatccag catgccgtca acgtcagcgt cggtcacttc
    20281 aacgatcggt ttttcaactt cgatcgcttc cagaccctgc agttcaactt ccggataaac
    20341 ttcaaactct acagagtaag tgaagtcttc acccagcttg tattcgcccg gaacataagt
    20401 cggtgcgcca gccggattga ttttttcttt aatgatggcg tcaatgaagt tacggctcat
    20461 caggtcaccc agaacgtcct ggcgtacaga cgcgccataa cgctgagcaa cgatattcat
    20521 tggcactttg cctttgcgga agccgtcaat acgtactttt ttcgcaacgt tgaccagctc
    20581 gcttttaaca gcggtctcga tgctgtcagc agcgatagta atcgttacac ggcggccaag
    20641 gccttgagtg gtttcaactg aaacttgcat cttgttacct caaaaaatca cagtgctcgg
    20701 tcaactctta ccccgcaagc acaggttgtt ggcttcgcgg aaccgggatg ttcttataat
    20761 caatcacatt ccctgttacc agaatcatcc cgaaagcatt caaataggac gcggcattat
    20821 agcggcatca cttttatgag tcgagaacgg ttattgcgcg ctgtcgcgta ttttttcaca
    20881 attcccgggc aattttcgtc tgaatgctgg gcaaaaagcg caaaatccag acaaaacaaa
    20941 acggcccgca ggccgttttt cataaaatct ttagcaacat actggaaaag cgccgacagt
    21001 tgcaaatgcg tttttacgcg atgcttcctg ctccacgaca gggaggagag gcaaagacgg
    21061 tgtcctgcaa cccttcccac tccttaatag tgtaagtatg cagagccagc gcatgaacgg
    21121 tagtagagag ttcctccgct aaagtactgt aaatcattcg atgacgattc agaaaacgtt
    21181 cacccgtaaa acgatcgctg accagcacaa ctttaaaatg gctttcagag ccggctggga
    21241 cattgtgacg atagctttca tccactactt cgaggaatac gggttggaac gccgccctta
    21301 atttttcttc tatccgctca cgtatcatca tgaaatatct cctccgacaa cgctgagatg
    21361 tcatccatcc ctttaaatac tagccgcttt taccgtttcc attgcagctt aacaacaaat
    21421 atttaggttt cgtcctggtt tttccgtcaa gcgtatgaag tttactgatt taaagattga
    21481 aacacaggcc tcgcatcagc gtttctttta ccaggggtga ttatttcgtc aacatgatga
    21541 atttacatgc cttacccact tcccctcgcc gttggcaatg ttatgatggc gggaattttt
    21601 accaaacacg cttaagagtc tctgagagcc cgaacatgtt caaaaaaatc ctcttcccgt
    21661 tagttgctct gtttatgctt gcaggatgcg caaaaccgcc aacaactatt gaagtttccc
    21721 cgacgattac actgccacag caggatccaa gcctgatggg cgtcaccgta agcattaatg
    21781 gtgccgatca gcgtaccgat caggcgctgg caaaagtcac ccgcgataat caaatcgtta
    21841 ccctgaccgc ctcccgcgct ctgcgtttcc tgctgcaaga agtgctggaa aaacagatga
    21901 ccgcgcgtgg ttacatggtt ggtccgaatg gcccggttaa tctgcaaatc atcgttagcc
    21961 aactgtatgc tgacgtgtcc cagggcaacg tgcgctacaa catcgctacc aaagcggata
    22021 tcgccatcat cgctaccgcg cagaatggca acaaaatgac caaaaactat cgtgccagct
    22081 acaacgttga aggtgcgttc caggcctcca acaaaaatat cgccgatgcg gttaacagtg
    22141 tgctgaccga taccatcgct gatatgtctc aggacaccag cattcacgaa ttcatcaagc
    22201 agaacgcgcg ttaatttctg ccctctggcc cggtgcaagc cgggcctgta gacgcccatg
    22261 tccagtcaat atttacgtat ttttcaacag ccgcgttcag ccatattgct gatcctgggt
    22321 tttgcttccg ggctaccgct cgccctgaca tccggcacct tacaggcctg gatgacggtc
    22381 gaaaatatcg atctcaaaac cattggtttc ttctctctgg taggccaggc ttacgttttt
    22441 aaattcctct ggtcaccgct gatggaccgc tacacgcccc cattttttgg gcggcggcgc
    22501 ggttggctgc tcgccacgca aatcctgtta ttagtcgcca ttgcggcgat gggttttctc
    22561 gaaccaggca cccaactccg ctggatggcg gcgctggcgg tggtgatcgc tttttgctct
    22621 gcctcccagg atattgtctt cgatgcgtgg aaaaccgatg tgctcccggc agaagaacgt
    22681 ggtgcgggcg cggcaatcag cgtgctgggt taccgtttag ggatgctggt ttccggcggc
    22741 ctagccctgt ggctggcaga taaatggttg ggctggcagg gcatgtactg gttgatggcg
    22801 gcgctgttga tcccctgtat tatcgcgacg ttgcttgcac cagaaccaac cgacaccatt
    22861 cctgtgccca aaacgctgga acaagcggtt gttgcacctc tgcgagattt ctttggtcgc
    22921 aataatgcct ggcttatttt gcttcttatc gtgctgtata agctgggcga cgcattcgcc
    22981 atgagcctga caaccacgtt tttgattcgc ggcgtcgggt ttgatgcggg tgaagtaggc
    23041 gtggtaaaca aaacgcttgg cttactggcg accattgttg gcgcattgta cggtgggatt
    23101 ttgatgcagc gcctgtcgct gttccgggcg ctgctgattt tcggcatttt acaaggtgcg
    23161 tctaacgctg gttactggct gctgtcgatt actgataagc atctctacag catgggcgca
    23221 gccgtctttt tcgaaaacct ctgtggcggg atgggcacat cagcctttgt cgcgctgtta
    23281 atgacgctgt gtaataagtc attttccgct actcaatttg ccctgctctc agcgctttcc
    23341 gctgtagggc gagtttatgt cggccccgtg gcgggttggt ttgttgaagc acacggctgg
    23401 tcgacattct atctattctc cgtcgccgct gccgtaccgg ggcttatttt gctgctggtt
    23461 tgccgccaga cgcttgaata tacacgagta aatgacaact ttatctcccg taccgaatat
    23521 ccggcaggtt atgcctttgc catgtggaca ctggcggcgg gcgtcagcct gttggccgtg
    23581 tggttacttc tgttgacgat ggacgcgctg gatttgacgc acttctcttt cctgcctgct
    23641 ctgctggaag tcggggtttt agtcgccctt tctggcgtca tgcttggtgg tttgctggat
    23701 tatctggcgc tacgaaaaac gcatctgacg taatctgtaa atattattta gctgtagtaa
    23761 tcactcgccg taattattac ggctaaataa acatcagcat cgcttattaa tatttgttct
    23821 tttgtgcggc ttagcgtttg gaaattattg acgccattta taaattctat ttttcttaca
    23881 cgattcagct aatgagtctt tattttctca ccacccagtt gtcactttaa tgataattat
    23941 ttgttaaata attgttttat ttcgcattgg ttataccaat tgcccgccaa gactccttct
    24001 gcactcccc
//
LOCUS       DAJTUN010000056        23804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTUN010000056 DAJTUN010000000
VERSION     DAJTUN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.056"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            484..2412
                     /gene="thrS"
                     /locus_tag="OO470_002083"
     CDS             484..2412
                     /gene="thrS"
                     /locus_tag="OO470_002083"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6251330.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2416..2958
                     /gene="infC"
                     /locus_tag="OO470_002084"
     CDS             2416..2958
                     /gene="infC"
                     /locus_tag="OO470_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6251331.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3055..3252
                     /gene="rpmI"
                     /locus_tag="OO470_002085"
     CDS             3055..3252
                     /gene="rpmI"
                     /locus_tag="OO470_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6251332.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3305..3661
                     /gene="rplT"
                     /locus_tag="OO470_002086"
     CDS             3305..3661
                     /gene="rplT"
                     /locus_tag="OO470_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6251333.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3778..3902
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3784..3828
                     /gene="pheM"
                     /locus_tag="OO470_002087"
     CDS             3784..3828
                     /gene="pheM"
                     /locus_tag="OO470_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6251334.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4111..5094
                     /gene="pheS"
                     /locus_tag="OO470_002088"
     CDS             4111..5094
                     /gene="pheS"
                     /locus_tag="OO470_002088"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6251335.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5109..7496
                     /gene="pheT"
                     /locus_tag="OO470_002089"
     CDS             5109..7496
                     /gene="pheT"
                     /locus_tag="OO470_002089"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6251336.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7501..7800
                     /gene="ihfA"
                     /locus_tag="OO470_002090"
     CDS             7501..7800
                     /gene="ihfA"
                     /locus_tag="OO470_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6251337.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7901..8881
                     /gene="btuC"
                     /locus_tag="OO470_002091"
     CDS             7901..8881
                     /gene="btuC"
                     /locus_tag="OO470_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6251338.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8944..9495
                     /gene="btuE"
                     /locus_tag="OO470_002092"
     CDS             8944..9495
                     /gene="btuE"
                     /locus_tag="OO470_002092"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6251339.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9495..10244
                     /gene="btuD"
                     /locus_tag="OO470_002093"
     CDS             9495..10244
                     /gene="btuD"
                     /locus_tag="OO470_002093"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6251340.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10322..10747
                     /locus_tag="OO470_002094"
     CDS             10322..10747
                     /locus_tag="OO470_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6251341.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            10994..11707
                     /gene="ydiV"
                     /locus_tag="OO470_002095"
     CDS             10994..11707
                     /gene="ydiV"
                     /locus_tag="OO470_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6251342.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11771..13207
                     /locus_tag="OO470_002096"
     CDS             11771..13207
                     /locus_tag="OO470_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6251343.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13211..13402)
                     /gene="hemP"
                     /locus_tag="OO470_002097"
     CDS             complement(13211..13402)
                     /gene="hemP"
                     /locus_tag="OO470_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6251344.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13534..14580)
                     /gene="aroH"
                     /locus_tag="OO470_002098"
     CDS             complement(13534..14580)
                     /gene="aroH"
                     /locus_tag="OO470_002098"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6251345.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14737..15570)
                     /gene="ppsR"
                     /locus_tag="OO470_002099"
     CDS             complement(14737..15570)
                     /gene="ppsR"
                     /locus_tag="OO470_002099"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6251346.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15902..18280
                     /gene="ppsA"
                     /locus_tag="OO470_002100"
     CDS             15902..18280
                     /gene="ppsA"
                     /locus_tag="OO470_002100"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6251347.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18337..19983)
                     /gene="fadK"
                     /locus_tag="OO470_002101"
     CDS             complement(18337..19983)
                     /gene="fadK"
                     /locus_tag="OO470_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6251348.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20040..20333)
                     /locus_tag="OO470_002102"
     CDS             complement(20040..20333)
                     /locus_tag="OO470_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6251349.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20330..21619)
                     /locus_tag="OO470_002103"
     CDS             complement(20330..21619)
                     /locus_tag="OO470_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6251350.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21675..22613)
                     /locus_tag="OO470_002104"
                     /pseudo
     CDS             complement(21675..22613)
                     /locus_tag="OO470_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22633..23397)
                     /locus_tag="OO470_002105"
     CDS             complement(22633..23397)
                     /locus_tag="OO470_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6251351.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5868 a         5812 c         6262 g         5862 t
ORIGIN      
        1 atgattaatt attatcgcca gaacgaaatg tgcacgaaaa attgttataa gaattgtgtc
       61 acttttatca ttgtgatatg accacttcgt cattaatctc atgagcgttt aaaatactac
      121 cgccaaatca cgcaacgaac tttgctggat tcagtttatg ctgctgtaaa tccgctcgca
      181 gtaaaccttt cagacgcacc gtgatgttat cagtggttct ttaagcgttt tgctggtgta
      241 ctcactacaa acgaattgcg aatcgaatca atgtgaaacg gaaaggtaca atctcccttt
      301 ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag gttaatgtct ttcatttgtg
      361 tacctgattg cgaaccaatt tagcatttgt tggctagatg gtttcgcaat gaactgttaa
      421 taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc accactgcaa ataaggatat
      481 aaaatgcctg ttataactct tcctgatggc agccaacgcc attacgacca cgctgtaagc
      541 cccatggatg ttgcgctgga cattggtcca ggtctggcga aagcctgtat cgcagggcgc
      601 gttaatggcg aactggttga tgcttgcgat ctgattgaaa acgacgcaca actgtcgatc
      661 attaccgcca aagacgaaga aggtctggag atcattcgtc actcctgtgc gcacctgtta
      721 gggcatgcga ttaaacaact ttggccgcat accaaaatgg caatcggccc ggttattgac
      781 aacggttttt attacgacgt tgatcttgac cgcacgttaa cccaggaaga tgtcgaagca
      841 ctcgagaagc ggatgcatga gcttgctgag aaaaactacg acgtcattaa gaagaaagtc
      901 agctggcacg aagcgcgtga aactttcgcc aaccgtgggg agagctacaa agtttccatt
      961 cttgacgaaa acatcgccca tgatgacaag ccaggtctgt acttccatga agaatatgtc
     1021 gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct gccatcattt caaactaatg
     1081 aaaacggcag gggcttactg gcgtggcgac agcaacaaca aaatgttgca acgtatttac
     1141 ggtacggcgt gggcagacaa aaaagcactt aacgcttacc tgcagcgcct ggaagaagcc
     1201 gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc tgtaccatat gcaggaagaa
     1261 gcgccgggta tggtattctg gcacaacgac ggctggacca tcttccgtga actggaagtg
     1321 tttgttcgtt ctaaactgaa agagtaccag tatcaggaag ttaaaggtcc gttcatgatg
     1381 gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact acaaagatgc aatgttcacc
     1441 acatcttctg agaaccgtga atactgcatt aagccgatga actgcccggg tcacgtacaa
     1501 attttcaacc aggggctgaa gtcttatcgc gatctgccgc tgcgtatggc cgagtttggt
     1561 agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc tgatgcgcgt gcgtggattt
     1621 acccaggatg acgcgcatat cttctgtact gaagaacaaa ttcgcgatga agttaacgga
     1681 tgtatccgtt tagtctatga tatgtacagc acttttggct tcgagaagat cgtcgtcaaa
     1741 ctctccactc gtcctgaaaa acgtattggc agcgacgaaa tgtgggatcg tgctgaggcg
     1801 gacctggcgg ttgcgctgga agaaaacaac atcccgtttg aatatcaact gggtgaaggc
     1861 gctttctacg gtccgaaaat tgaatttacc ctgtatgact gcctcgatcg tgcatggcag
     1921 tgcggtacag tacagctgga cttctccttg ccgtctcgtc tgagcgcctc ctatgtgggc
     1981 gaagacaacg agcgtaaggt accggtaatg attcaccgcg caattctggg gtcgatggag
     2041 cgtttcatcg gtatcctgac cgaagaattc gctggtttct tcccgacctg gcttgcgcca
     2101 gttcaggttg ttatcatgaa tattaccgat tcacagtctg aatacgttaa cgaattgacg
     2161 caaaaactat caaatgcggg cattcgtgtt aaagcagact tgagaaatga gaagattggc
     2221 tttaaaatcc gcgagcacac tttgcgtcgc gtcccatata tgctggtctg tggtgataaa
     2281 gaggtggaat caggcaaagt tgccgttcgc acccgccgtg gtaaagacct gggaagcatg
     2341 gacgtaaatg aagtgatcga gaagctgcaa caagagattc gcagccgcag tcttaaacaa
     2401 ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgccc taaccgtatc
     2461 aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc tggaaggcga gcagcttggt
     2521 attgtgagtc tgagagaagc tctggagaaa gcagaagaag ccggagtaga cttagtcgag
     2581 atcagcccta acgccgagcc gccggtttgt cgtataatgg attacggcaa attcctctat
     2641 gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa aagttatcca ggttaaggaa
     2701 attaaattcc gtcctagtac agatgaaggc gactatcagg taaaactccg cagcctgatt
     2761 cgctttctcg aagagggtga taaagccaaa atcacgctgc gtttccgcgg tcgtgagatg
     2821 gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga aagacgattt gcaagaactg
     2881 gcggtagtcg aatccttccc aacgaagatc gaaggccgcc agatgatcat ggtgctcgct
     2941 cctaagaaga aacagtaagg ccttcaagta gcactatctg tggagccttt gggcttcaca
     3001 ggcattgttc gcctttgttt cgtttattaa caatgcgaag tggaagttat taaaatgcca
     3061 aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa aggtggtttt
     3121 aagcacaagc acgctaacct gcgtcacatt ctgactaaaa aagcgaccaa acgtaaacgt
     3181 cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat cgcgtgcctg
     3241 ccgtacgcat aagccgttaa cgtttttaac tttttaatta gaatatagat acaggagagc
     3301 acatatggct cgcgtaaaac gtggtgttat tgcacgcgca cgtcacaaga aaattttgaa
     3361 acaagctaaa ggctactacg gtgcgcgttc tcgcgtatac cgcgttgcct tccaggctgt
     3421 tatcaaagct ggtcagtatg cttaccgtga ccgtcgtcaa cgtaagcgtc agttccgtca
     3481 actgtggatt gcgcgtatca acgcagcagc acgtcagaac ggtatttctt acagcaaatt
     3541 catcaatggc ctgaaaaaag cctctgttga aatcgaccgt aagatcctgg ctgatatcgc
     3601 agtattcgac aaagtagcgt tcaccactct ggttgaaaaa gcgaaagcag ctctggcata
     3661 agccagttga aagagggagc tagtctccct cttttcgttt caacgccatc aaaacattga
     3721 cttttatcgc cgtagccttt tcaataaagg tcttttgaag agtaccaaaa ggtaacgcaa
     3781 gcaatgaatg ctgctatttt ccgcttcttt ttttacttta gcacctgaat ccaggaggct
     3841 agcgcgtgag aagagaaacg gaaaacagcg cctgaaagcc tcccagtgga ggcttttttt
     3901 gtatgcgcgt tttgaaaaat aaaaagccag tctatcaggc gtaattgtcg cagccagtct
     3961 ggacacggcc agtgcggaga aaccggagcg tacttaagta cgtgaggatt tcgagcactg
     4021 cccgggacca aaatggcaag taaaatagcc tgatgggata ggctctaagt ccaacgaacc
     4081 agtgtcacca ctgacacaat gaggaaaacc atgtcacatc tcgcagaact ggttgccagt
     4141 gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt gcgcgtcgaa
     4201 tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga gctgccgcca
     4261 gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt tcagcaggcg
     4321 ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct ggcggcggaa
     4381 acgattgatg tctctctgcc aggtcgtcgc attgaaaacg gcggtctgca tccggttacc
     4441 cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg
     4501 ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg tcaccacccg
     4561 gcgcgcgctg accacgacac tttctggttt gacgctaccc gcctgctgcg tacccagacc
     4621 tctggcgtac agatccgcac catgaaagcc cagcagccac cgattcgtat catcgcgcct
     4681 ggccgtgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca tcagatggaa
     4741 ggtctgattg ttgataccaa catcagcttt accaacctga aaggcacgct gcacgacttc
     4801 ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta cttcccgttt
     4861 accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg gtaaatggct ggaagtgctg
     4921 ggctgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc ggaagtttac
     4981 tctggtttcg ccttcgggat ggggatggag cgtctgacta tgttgcgtta cggcgtcacc
     5041 gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa ataaggcagg
     5101 aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg gcgattgata
     5161 gcgatacgct ggcgaatcaa atcactatgg cgggcctgga agttgacggt gtagaaccgg
     5221 ttgccggtag cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg cagcatccga
     5281 acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg ctggacatcg
     5341 tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagctacc attggtgctg
     5401 ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg tggcgaaccg tctgaaggga
     5461 tgctgtgctc cttttctgag ctgggaattt ctgacgatca cagtggcatt atcgaactgc
     5521 ctgcggatgc gccgattggc accgacatcc gcgaatacct gaaactcgat gataacacca
     5581 tcgaaatcag cgtgacacca aaccgtgccg actgcttagg tatcattggt gttgcgcgtg
     5641 acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga aatcgttccg gttggtgcga
     5701 ccatcgacga cacgttgccg attacagtcg aagcgccgga agcctgcccg cgctatcttg
     5761 gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg aaagaaaaac
     5821 tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac tatgtactgc
     5881 tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc ggcattgtgg
     5941 tgcggatggc aaaagagggc gaaacgctgg tgctgcttga tggcactgaa gcgaagctga
     6001 atgctgacac tctggtcatc gccgaccaca acaaggcgct ggcgatgggc ggcatctttg
     6061 gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cttgctggaa tgcgctttct
     6121 tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact gatgcgtctc
     6181 accgttatga gcgtggcgtt gatccggtac tgcagcacaa agcgatggaa cgtgcgaccc
     6241 gtctgctgat cgacatctgc ggtggtgagg ctggcccggt aattgatatc accaacgaag
     6301 caacgctgcc gaagcgtgca accatcactc tacgtcgtag caaactggat cgcctgatag
     6361 gccatcatat tgctgatgag caggtaactg acattctgcg tcgtctcggc tgcgaagtga
     6421 ccgaaggcaa agacgaatgg caggcagttg cgccgagctg gcgtttcgat atggagattg
     6481 aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc ccggatgagc
     6541 cggtacaggc aagcctgatt atggggactc accgtgaagc tgacctgtcg ctcaagcgcg
     6601 tgaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc ttcgttgatc
     6661 cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctggca agcccgatct
     6721 ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg cctgctggca accgtggtgt
     6781 acaaccagca ccgtcagcag aaccgtgtgc gcattttcga aagcggtctg cgtttcgtac
     6841 cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt gtgatttgcg
     6901 gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc tatgatttga
     6961 aaggcgacct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt gagttccgtg
     7021 cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg aaaggtgaac
     7081 gtattggttt tgttggagtt gttcatcctg aactggaacg taaactggat cttaacggtc
     7141 gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg cctcaggcgc
     7201 gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc gcagaaaacg
     7261 ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag gtagttggcg
     7321 taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag agcctcgcca
     7381 taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt gccgctaccg
     7441 tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg gattgaacct
     7501 atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg gcttagcaag
     7561 cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc tctggaaaac
     7621 ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa gaatcaacgc
     7681 ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg cgtggtgacc
     7741 ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa agacgagtaa
     7801 tctgatctaa ctaaaaaggc cgctatgcgg ccttttttct tttcactgtc gaagagtcac
     7861 cgtaaaatca acgccatgac acttcagcag aacggatacc atgctgacac ttgcccgcca
     7921 acaacagcga caaaatattc gctggttatt atgcctgtca gttttgatgc tgctggcgct
     7981 tctcttaagc ctttgcgccg gtgaacaatg gatttcgcca ggtgactggt ttactcctcg
     8041 tggcgaactg ttcgtctggc aaattcgcct gccacgtacg ctggctgtat tgctggttgg
     8101 tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc ctctggcaga
     8161 acctggacta cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg cggtattgct
     8221 tgggcaaggg caactcccca actgggcgct agggctgtgt gcgattgctg gcgcgcttat
     8281 catcacttta atactcttac gtttcgcccg tcgtcatctt tcgaccagtc ggttattgct
     8341 ggctggcgtt gcattaggga ttatctgcag cgcactaatg acgtgggcta tctacttttc
     8401 cacctctgtt gatttacgtc agctgatgta ctggatgatg ggcggttttg gcggcgtaga
     8461 ctggcggcaa agctggctga tgctggcatt gatccccgtg ttgttgtgga tctgttgtca
     8521 gtccaggccg atgaatatgt tagcacttgg cgagatctcg gcgcggcaac tgggtttacc
     8581 catgtggttc tggcgcaatg tgctggtggc agcgaccggc tggatggttg gcgtcagtgt
     8641 ggcgctggcg ggtgctatcg gctttattgg tctggtgatc ccacatattc ttcggttgtg
     8701 tggtttaacc gatcatcgcg tattacttcc cggctgcgcg ctggcagggg cgagcgcatt
     8761 gctgctggcc gatattgtag cgcgcctggc attagctgcc gcagagctgc ctattggcgt
     8821 ggtcaccgca acgttgggtg cgccggtgtt tatctggtta ttgttaaaag caggacgtta
     8881 gccgcaaaaa gacggtctat gattaaaagc taagattttt actgaccaca cctaggagaa
     8941 acgatgcaag attccattct gacgaccgta gtgaaagata tcgacggtga agtgaccacg
     9001 ctggagaagt acgccggtaa tgtgctgtta attgtcaatg tcgcctcaaa gtgtggctta
     9061 acgccgcaat atgagcagtt ggagaatatt caaaaagcct gggccgatcg aggttttgtg
     9121 gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac cgggcagcga tgaagagatt
     9181 aaaacttact gtaccaccac atggggggtg acgttcccga tgttcagtaa gattgaagtt
     9241 aatggcgaag gacgccatcc gctgtatcaa aaattgattg ccgcagcgcc gaccgcagtc
     9301 gcgccggaag agagcggatt ctatgcccgt atggtcagca aaggccgtgc accgctgtac
     9361 ccggatgata ttttatggaa ttttgaaaaa ttcctggttg gcagggacgg aaaagtcatc
     9421 cagcgttttt ccccggatat gacgccggaa gatcccattg tgatggaaag cattaaactg
     9481 gcgttggcaa aataatgtct attgtgatgc agttacagga tgttgcggaa tctacccgcc
     9541 tggggccgct ttcaggcgag gttcgggctg gggaaatcct gcacttggtg gggccgaatg
     9601 gcgcgggtaa gagtacctta ctggcgcaaa tggccggaat gaccagcggt aagggaagca
     9661 ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac aaaactcgcg ctgcatcgcg
     9721 cctatctttc acaacagcag acgccgccgt ttgcaatgcc ggtctggcac tacctgacac
     9781 tgcatcagca cgataaaacg cgtaccgaac tactgaatga tgtcgcaggg gcgctggctc
     9841 ttgatgacaa actcggtcgt agcaccaatc aactttccgg cggtgaatgg caacgcgtac
     9901 gtcttgctgc ggtggtgttg caaatcacac cacaagccaa tcccgcaggc caattgctgc
     9961 ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca aagtgcgtta gacaaaattc
    10021 tgagcgcgct gtgtcagcaa ggactggcga ttgtgatgag cagtcacgat ctcaaccaca
    10081 cgttgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg aaaaatgctg gccagtggac
    10141 gcagagaaga ggtgctcacg ccgccaaatc tggcgcaggc ctatgggatg aattttcgcc
    10201 gtctggatat cgaaggtcac agaatgctga tttcgaccat ctgatttcat tagtatcccg
    10261 aaaatcgggt aattacgacg ctaaattaat gcaagaataa aaaacagagg aaccgtacgg
    10321 aatgcgtttc tgccttattt tgatcacagc actgtttctg gccgggtgta gccaccataa
    10381 agcaccgccg ccaaatgcca gactttctga ttcgattacc gttattgccg gtttgaacga
    10441 ccagctacaa agctggcatg gcacgccgta tcgttatggt ggcatgacgc ggcgcgatcg
    10501 tttcgatttg catctgcccc gtgaaaccaa acaacaagcc tctattggca cgcaaattga
    10561 taaagacgag ttgctgcctg gtgacctggt ctttttcaaa acgggttccg gacaaaatgg
    10621 tttgcatgta ggtatttacg ataccaacaa ccaatttatc cacgcctcta ccagcaaggg
    10681 agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc aggcgagacg
    10741 aatctagtat gtcgaaaaat tgacggcgat gatacatagc cgtcaattta ctcctgaata
    10801 cagtagtatt ttgcagacaa aaattccgta aatggattat tcgtttataa tcaggataat
    10861 ctaaaaaaag gccccctgaa aaagtggtta acatggaaca aatgaaagtt actccttctg
    10921 taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac atccttcgag
    10981 agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt gctatttcct
    11041 gccgataaga gataaccagc aactccttgt cggcgttgag ttaattaccc atttctccag
    11101 tgaagatggc acggttcgta tcccgacgag tcgggttata gcgcaactta ccgaggagca
    11161 acattggcaa ttattttcag agcagttgga attactaaaa tcatgccaac acttttttat
    11221 tcaacataaa ctctttgcct ggcttaattt aacgccacaa gtggctacat tattattaga
    11281 cagggataat tacgcaggcg aactattaaa atatccgttt attgagctgt tgattaatga
    11341 gaattacccg catctcaatg aaggaaaaga taatcgcgat cttttgtcct tatcgcaaat
    11401 gtatcccctt gtgttaggca atttgggagc aggtaatagc acaatgaaag cggtttttga
    11461 tggtcttttc acccgcgtaa tgctggagaa aggttttatt cagcaacaaa tcacgcatcg
    11521 ctccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccttgtt gcaattgcat
    11581 catcgcgggg ggcattgata cgccagaaat attggcacag attaccccct tcgatttcca
    11641 tgctctccag ggatgcctgt ggcctgccgt tcctataaat cagataacga cgttggttca
    11701 gcgataaccc tcctgtttgc tggtgtttaa gacgagagta accgtctaca ctatcaaaca
    11761 gataggatct atgaccctgt cttttattac ccgctggcgc gatgaattgc cagaaaccta
    11821 tacagcactt tcccctacgc ctttaaataa tgcccggctg atttggcata ataccgaact
    11881 cgctaacacg ctgagtattc catcgtcgct gtttaaaaat gccgcaggtg tctggggcgg
    11941 cgaaacccta ctgcctggta tgtcaccact ggcacaggtt tacagtggtc atcagttcgt
    12001 cgtctgggcg ggccaactgg gtgatgggcg cggcatttta cttggcgaac aactgcttgc
    12061 tgatggcact acaatggact ggcatctgaa aggtgctggc ctgacgcctt attcgcgaat
    12121 gggtgatgga cgggcggttt tacgttcgac gatacgagaa agtctcgcca gtgaggcgat
    12181 gcattatctg ggcattccga cgacccgcgc gttaagtatc gtcaccagcg attcgccagt
    12241 gtatcgggaa acggtggagc caggcgcgat gctgatgcgt gtggcaccaa gtcatctgcg
    12301 ctttggtcat ttcgaacatt tttactatcg ccgcgagccg gaaaaggttc gtcagttggc
    12361 tgactttgcc attcgtcatt actggtcaca tcttgaagac gatgaggaca aataccgtct
    12421 ctggtttaac gatgttgtcg cacgtaccgc gtcgttaatt gcccaatggc agacggtcgg
    12481 ctttgcacat ggggtgatga ataccgacaa catgtcgctg ctgggactga cgcttgatta
    12541 cgggccgttt ggttttcttg atgattacga acccggtttt atttgtaatc actcggatca
    12601 tcaagggcgt tatagctttg ataatcaacc tgctgtcgcg ttgtggaatt tacagcgtct
    12661 ggcgcagaca ttgtcaccat ttgttgccgt agatgccctg aatgaggccc tggacagcta
    12721 tcagcaggtt ttgttgacgc attatggaca acggatgcgg cagaaactgg gcttcatgac
    12781 ggagcaaaaa gaggataacg cgctactgaa tgaattattc agtctgatgg cgcgagagcg
    12841 cagcgattat acccgcacat tccgcatgct gagtctgacc gagcagcaca gcgcggcgtc
    12901 accgctacgt gaggagttta ttgatcgtgc ggcatttgat gactggtttg cccgttatcg
    12961 ggggcgtttg caacaagacg aggttagcga tagtgagcgt cagcaactga tgcaaagcgt
    13021 taacccagct ctggtattgc gcaactggtt ggcgcaacgg gcgattgaag cggcagaaaa
    13081 gggtgatatg atggaattgc accgcctgca tgaggcgttg cgaaatcctt tcagcgacag
    13141 agatgatgac tatgtcagtc gtccacctga ctggggtaaa cggctggaag tcagctgctc
    13201 gagttaaagg ctacttagtt aacagcagct tgccagcctg agttttacgg agcagatatt
    13261 cttgcccgtc atgatcgata atcagtttgc catccggacc taacagtgtc tggctggtaa
    13321 ttcgccggat gggctgcggg gaagcggtct tgtgattggc atccgtttca ggcgtgagtt
    13381 ttctgctatc cgtataacgc ataaaaatta ataataattg atataacaat gatttctatt
    13441 atcaatatta tggcaggcca gagcaagcag taatgtgttg agaagacaac aagttgcgca
    13501 gaaatgccca tcccatcagg aatgggcacg cattcagaag cgggtatcta ccgcaaaggc
    13561 gagtttttcg accaggcgtt cggtatcttc ccagcccaga cacgggtcgg taatggattg
    13621 accgtaagtg agcggctgac cgccgacgat tttttgcgtt ccttcgcgca ggaaactttc
    13681 cgccataatt ccagcaatct ccgtagagcc attgcggatt tgctgacaaa tatcctcaca
    13741 aacttctaac tggcgacggt gctgcttctg gcagttaccg tggctgaaat ccaccaccag
    13801 atgttcaggt aaatcaaact cgtgcagcgt atcgcaggct gcggcgatat catcggcatg
    13861 ataattcggt tttttgccgc cacgcataat aatgtggcca tacgggttgc cgctggtctg
    13921 atagatggtc atctgaccat ttttgtctgg cgagaggaac atatggctgg cgcgggctgc
    13981 gcggatagca tccacggcaa tccgcgtgtt gccatcggta ccatttttaa aacctaccgg
    14041 acaggagagt gctgaagcca tttcgcggtg gatctgactt tcggtagtac gagcgccaat
    14101 cgcgccccaa ctgattaaat cagcaataaa ctgaccggtt accatatcga ggaactcagt
    14161 cgcggtaggg acacccagct catttacctg taaaagtaat ttgcgcgcca gctccagacc
    14221 gtgatttacc cgatagctgc cgtttaaatc cggatcggag attagccctt tccagccgac
    14281 aacagttcgt ggtttttcaa aataggtgcg cattacgatt tccagccgtg actggtactg
    14341 gttgcgcagc gactgcagac gggtggcgta ctctattgca gcggtgagat cgtggatcga
    14401 gcaggggcca ataatgacca acagtcgctt atcttcacca ttcagtattt tttcaattct
    14461 gcggcgggag tcggtgacat gggtggcgac gccaggcgtt acgggatacc gtagcgcgag
    14521 ttcggcgggc gttaccaggc tctcaatacg cgcagtacgg agttcgtcag ttctgttcat
    14581 tacaagtctc agaaatttgt tgcttctacg gcaggactac cggaaagtga tgcgcatcac
    14641 gataaaccaa tcgcagctga tttcaagtgc gaggtgtgtc acacctcgcc gggtggttag
    14701 catgataaca aaaaaacaaa ccaatgcact agttctctag tacattcggc gactaaggcc
    14761 catgatatcg aggatcttgg tggcaatctc ttctaccgaa taattggtac tgttaatcca
    14821 cgggatctga tttttacggt acaaggcttc cacttccgcg acttccatcc tgcactgacg
    14881 aagcgaggca tagcgactgt tctcccgacg ttcctcgcga atcgccgcca gacgttccgg
    14941 gtcgatagtc aggccgaaca atttatgctg aagcggtttg agcgacgcgg gtagcaccag
    15001 attatccata tcgtcggcaa taaaggggta gtttgccgcg cggataccaa attgcattgc
    15061 cagatacaga ctggtggggg ttttaccaca gcgagaaaca ccgagcagga tcacctgagc
    15121 ctgatccaga ttgcgcaacg aaatgccgtc atcgtgggcg agggtgtaat caatcgccgc
    15181 aatgcgcgca tcatatttat tgagattatt agggttaagg ccatgggtac gatgagcaat
    15241 cggcgttgga tccagtttca tctcttgttg tagcggggca accagcgcct gaacgatatc
    15301 ctggcaaaag ccttcacttt gcaagatgat ggcgcgaatc tccggcaaca cgatggagta
    15361 gaagaccagc gggcgcacgc ctgtctggtg ataaattgcg tcaatctgat ccttcactgc
    15421 ccgtgcacgg ctctcatttt cgacaaacgg cagcgtgatg ctgctgatag tgacgggaaa
    15481 ttgtgacatt actgcgtgtc ctaatacctc cgcagttatt gccgtaccat cagaaatata
    15541 aaaaacgtgg cgatcaacag cattatccat tttgttcttc ccgtgaagca gacataattc
    15601 tatgaaagca taaattaaaa gcgcacagaa gcgtaggacg taatgtctgg tttataaaat
    15661 gaaccttcat tttatttttt ataaaaacag catttcattt ttatggtttc gtttataccg
    15721 atggtttatg tggaaattgt cgaatagagc aggtttgcgc aacgctggga tcagtcttaa
    15781 aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc
    15841 aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgttc
    15901 gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga
    15961 tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta atctttccgg
    16021 aatgggtgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct
    16081 ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga
    16141 tgttactcag cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt
    16201 ccagcctgag ctggaaaacg ccatacgcga agcctatgca cagctttccg ccgatgacga
    16261 aaacgcctct tttgcggtac gctcctccgc caccgcagaa gacatgccgg acgcttcctt
    16321 tgccggtcag caggaaacct tcctcaacgt tcagggtttt gacgccgttc tcgtggcagt
    16381 gaaacatgta tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg
    16441 ttacgatcac cgtggcgtgg cgctctccgc tggtgttcag cggatggtgc gctccgacct
    16501 cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt
    16561 tatcacttcc gcatggggcc ttggggaaat ggtcgtgcag ggtgcggtta acccggatga
    16621 gttttacgtg cataaaccga cactggcggc gaatcgcccg gctatcgtgc gccgcaccat
    16681 ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa
    16741 aatcgaagac gtaccgcagg aacagcgtga catcttctcg ctgaccaacg aagaagtgca
    16801 ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccaa tggatattga
    16861 gtgggcgaaa gatggccaca ccggcaaact gttcattgtg caggcgcgtc cggaaaccgt
    16921 gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc
    16981 cgaaggccgt gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtca tccatgacat
    17041 cagcgaaatg aaccgcatcg aacctggcga cgtgctggtt actgacatga ccgacccgga
    17101 ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg
    17161 tcacgcggcg atcatcgctc gtgaactggg cattccggcg gtagtgggct gtggagatgc
    17221 aacagaacgg atgaaagacg gtgagaacgt cactgtttct tgtgccgaag gtgataccgg
    17281 ttacgtctat gcggagttgc tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc
    17341 ggatctgccg ttgaaagtga tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc
    17401 ctgcctgccg agcgaaggcg tgggccttgc gcgtctggaa tttatcatca accgtatgat
    17461 tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga
    17521 aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac
    17581 tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc
    17641 tgattttaaa tcgaacgaat atgccaacct ggtcggtggt gagcgttacg agccagatga
    17701 agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca gcttccgcga
    17761 ctgcttcgca ctggagtgtg aagcagtgaa acgtgtgcgc aacgacatgg ggctgaccaa
    17821 cgttgagatc atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg tggttgaaga
    17881 actggcgcgt caggggctga aacgtggcga gaacgggctg aaaatcatca tgatgtgtga
    17941 aatcccgtcc aacgccttgc tggccgagca gttcctcgaa tatttcgacg gcttctcaat
    18001 tggctcaaac gatatgacgc agctggcgct cggtctggac cgtgactccg gcgtggtgtc
    18061 tgaattgttc gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg ctatccgtgc
    18121 cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg accacgaaga
    18181 ctttgccgca tggttgatgg aagaggggat cgatagcctg tctctgaacc cggacaccgt
    18241 ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatccc cggcggcgtt
    18301 cagtcgccgg ggttatgtga tccccgaaga tgaaacttat tcaatctctt cacagacatc
    18361 ctgcgttaat cgccgtataa tatcttttct taacaaaaac ttttgtattt tatctgaggc
    18421 agtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt ttgcgacccg
    18481 tttacggcta aaaaaagccg ccatctcttc cagcgataat gaatgatgcg gcgctttcag
    18541 cacgacataa gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa ccacacaggc
    18601 atcgtgaatt ttaggatgct gcaataaaat atcttccact tcacggctgc taatattttc
    18661 gccgccgcgg acaataatat cttttttgcg tccggtaatt tttatatagc cagcctcatc
    18721 catacggcag agatcgccgc tgtaatacca gccttcttca tccagcgcac gggcggttaa
    18781 ttcaggttca tcaaaatacc ccataaacac attggggcca cgcgatgctt cttcaccttc
    18841 gcaacctggc ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc
    18901 agcgtaacca tcggtatgca taaagcgcga caaagaatta tcgagattca ccaccgcatg
    18961 cggcgaactt tctgtggaac catagacact taataattta atgctgcgct gctggcattc
    19021 gcgcgccact tttttgggga ttgtggtacc gccgcaaaga aagaagcgca gcgctgaaag
    19081 gtctgcgggt tgtttctcta gtaaattcaa aagatcatag acaaacggcg ttgcgcctag
    19141 catacaggtg caacgctgct gctcaagcag cgcgagacac gcatcaggag tgaaaatatc
    19201 taacaacacg ctgcgagcgc caattaagaa tggtgccgtt acgccatgca gaaagcccgt
    19261 tgcgtgacca agtggcgcag gcatcataaa gacatccagc caggtcagat tcagccgcgc
    19321 gcaataagcc cgctcactgg cgagaatatt gttatgcgtt agcatcacgc cctttggcag
    19381 accctcggtt ccggaggtaa acagcaccgc agctaattca tcgccgtggg tcgttatcgc
    19441 cgtggtcagt gggatattgt cggcgataat ctgacttaat gagagggaag aggtggcggg
    19501 agccagtttg tccacgccga caatttgttg tagttgtgga agctgatttt gcagcggcag
    19561 gattaaatct accggacgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg
    19621 acacttattg agcacccaca ccagttctgc ttcccgccag gaaggcaaca gcggcacgga
    19681 aaccgcaccg attttcaggc aggcaagata gataacggta aattcacacc agccaggcag
    19741 ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca
    19801 gctcgcggcg tgatcgagcg cgctataggt gtacgatgca ccatgattat cgaccacggc
    19861 aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagtg aagcatcgcc
    19921 ccatagccct tgctgacgat acgccgcacg acgttgttcg ttaaacgtta atgtcacttt
    19981 cattttggac tctcgaaaca gaacatcagg cccgagttgc gggcctgtgg gatgcatgat
    20041 tagccgtagc gaaaatcaat accgaaggtg cctgcgggat attgccactg ttcgagaata
    20101 gtgttaccgc acagcacccg acaggtgccg cactccagac aaccggcggg atcaaaatga
    20161 atgtttccta cgtcatcctg cttataaagt ccggcagggc aggctttcat taatttacgg
    20221 aattcattga tatcgggatt ttccgtcaga atgatatgcg ggtggccctc atcaacatgg
    20281 aatttattga cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg
    20341 ttgctccctt aatgccatct ttcagcaagt tgatcagccc aattttcttc gcgtgtccca
    20401 tgatcatttt gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc tcgttcatga
    20461 tgtcggctac cattcgtggg tattggctaa acaggcgcgg gttttccatc agcgccggga
    20521 tcttgcgaaa atgctgcata tcacgcatga cgcagctttg ttccagctca cgtttgtatt
    20581 gcgccagact gttggcggag aaatccgcgc gttctttggc ggcgatcacc gttgtggcgg
    20641 cagcctgagc cgatgcaatg gctaaatcca tgccgcggac cgtaaaaccc aaattcaggc
    20701 agaagcctgc ggcgtcacca acgatcatca cgccctcgtt aaccagttgc ggcaccattg
    20761 ccagaccgcc ttccggcacc atatgcgcgg aatattcaag cagtttgccg ccgctaatca
    20821 gcgggcgaat ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg cttttttgcg
    20881 catgggcgat atcacccaat ccacaaacca gccccaagga tatggaatcc ttgttggtat
    20941 agagaaatcc cccgcccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac
    21001 cttcctcgcc cgtaatatta aagcgatcgt tgatctgttc tggtgtgagg ccaataacct
    21061 ctttaacacc aacagcgtaa tgatgcggat cggaagcggg aaccattccc agcgagcggc
    21121 caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga atatcatccc
    21181 cagcctgcac gccagtgacc ttgtttcctt cacgaaccag cgcgtcgacg cgaacgcctg
    21241 ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc agacgattac
    21301 gcaatacggt ataagatgcg tgttgaggaa catctggttg ctcgcggtga aaatcgaggg
    21361 taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg
    21421 gcgctgatac tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa agacgcccgc
    21481 cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg
    21541 ctcgtgccat gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc acaatggcat
    21601 caaatttgtc atccgacatg cgttatgctc ccttcctgct gcgtttacag cgggagatgt
    21661 ggttgaaagg ggaattagcg gctcaactgg ctaatcaggg cagggacgac tttgtagata
    21721 tcgcccacca gaccgtagtc ggcatagttg aagattggcg catttttatc tttattgatg
    21781 gcgacaatca cttttacgcc gttgccgcca accatatgtt ggatctgccc ggagatcccc
    21841 agcgtcaggt agagatcgga tttcagcaac acgccggaga caccgatata acgttcacgc
    21901 tccatccagt tctcgccttc ggcaattgga cgtgaacagc cgacttcagc attcagcacc
    21961 gccgccagtt cgtggaccat tttcaggtca tcctgcgccg ccagaccacg accgacgcca
    22021 accacacgtt ttgctttgct caggtccacg ctgcttgcgg ctttcgcacg gcgttcgcga
    22081 cagagaattt catgacgcgg agcaacataa ggtaccgttt ctgtcgggca ctgatgagag
    22141 gtatcactgg tgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc cagcgggctg
    22201 ttgatctttt cctaagcgaa cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga
    22261 ccatcgacaa tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag ttgcacactt
    22321 aaccgtgctg ccagagcttt accacgtttc gtcgcggcca acagcaacat agcggggtgt
    22381 ttatctttca gcagggcagc aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg
    22441 ttttgctcaa gaacataaat acattttgga ccataaggca taactgcctg cgcctggtcg
    22501 gtattttgta caatggcata cacctgttgg ccccattgct gagcgccgcc aaacagttca
    22561 gcataacgtt caggattatc gctaaagacc cagacgctgt ttaattgact catcgtttct
    22621 tctccgtagg cttcagttca gggctttctt cagatgttcc gccagctcgg caatggcctc
    22681 cggcgaatcg ttatcgagaa tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc
    22741 gaccagttca gcaagtggcg cgctctggct ccagtcaata tcacttgcct gccactgatt
    22801 taccggtttt ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg gcacgttaat
    22861 atcggaggtg acgcagagca cggctggaac agagagttca ataacttcaa catcatcttc
    22921 aagcgtgcgt tcaatcacca gtgtattgcc ctgacgctga atagcactca ctgcattaat
    22981 caccggaagt tgcagaattt ctccgaccag caagccaacc tgctgggcat aaaggtcgcc
    23041 ggaaccttca ccaaagatca gtaaatcgaa gccgatcttt tcaattgctg ccgccagcgc
    23101 ttttgcggta tcgagaggca gtgcatgttc aagttgcgca tcctgcacca aatacaggct
    23161 gtgcggcccg cgggatagca cgtctttgcg cactttcgag ttctgcaaca atgagccgcc
    23221 aacggtcagc gcggctatct catcgtcatc tgttgcgagc tggcttgcag cttcaatggc
    23281 attgagatcg aactggctga ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg
    23341 agtgacaaca atgtcctgtt cttcaggcac cagcttaaag caggttatta ttttcatgat
    23401 ttctcctgtg agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata
    23461 gtgtgcgata gcggtgcggg gggttcgacg gagcaagctg ttttccttgc caacaatctt
    23521 gcaaaaaaaa tgcgcttgct aaaacaaaag tgcaatttgc ggcaatatag ttattccatg
    23581 accgtacaat cttgcattca gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt
    23641 ataaccgcaa tacacgccgt attttatccg gcatatattg tttttgcttg ctcatggaat
    23701 agggaggttg tcatgtatca acgctgtttt gataatgcca gtgaaacgct gtttgtcgcc
    23761 ggtaaaacgc cacggctttc acgttttgca tttagcgatg atcc
//
LOCUS       DAJTUN010000057        23743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTUN010000057 DAJTUN010000000
VERSION     DAJTUN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.057"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            114..1763
                     /locus_tag="OO470_002106"
     CDS             114..1763
                     /locus_tag="OO470_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6251352.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(1917..3209)
                     /locus_tag="OO470_002107"
     CDS             complement(1917..3209)
                     /locus_tag="OO470_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6251353.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHLNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(3387..5036)
                     /gene="actP"
                     /locus_tag="OO470_002108"
     CDS             complement(3387..5036)
                     /gene="actP"
                     /locus_tag="OO470_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6251354.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(5033..5347)
                     /locus_tag="OO470_002109"
     CDS             complement(5033..5347)
                     /locus_tag="OO470_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6251355.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(5547..7505)
                     /gene="acs"
                     /locus_tag="OO470_002110"
     CDS             complement(5547..7505)
                     /gene="acs"
                     /locus_tag="OO470_002110"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6251356.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            7898..9334
                     /gene="nrfA"
                     /locus_tag="OO470_002111"
     CDS             7898..9334
                     /gene="nrfA"
                     /locus_tag="OO470_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6251357.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            9379..9945
                     /gene="nrfB"
                     /locus_tag="OO470_002112"
     CDS             9379..9945
                     /gene="nrfB"
                     /locus_tag="OO470_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6251358.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            9942..10613
                     /gene="nrfC"
                     /locus_tag="OO470_002113"
     CDS             9942..10613
                     /gene="nrfC"
                     /locus_tag="OO470_002113"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6251359.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            10610..11566
                     /gene="nrfD"
                     /locus_tag="OO470_002114"
     CDS             10610..11566
                     /gene="nrfD"
                     /locus_tag="OO470_002114"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6251360.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            11586..13304
                     /locus_tag="OO470_002115"
     CDS             11586..13304
                     /locus_tag="OO470_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6251361.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            13297..13680
                     /gene="nrfF"
                     /locus_tag="OO470_002116"
     CDS             13297..13680
                     /gene="nrfF"
                     /locus_tag="OO470_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6251362.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            13677..14273
                     /gene="nrfG"
                     /locus_tag="OO470_002117"
     CDS             13677..14273
                     /gene="nrfG"
                     /locus_tag="OO470_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6251363.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            14615..15928
                     /gene="gltP"
                     /locus_tag="OO470_002118"
     CDS             14615..15928
                     /gene="gltP"
                     /locus_tag="OO470_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6251364.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(16006..16695)
                     /locus_tag="OO470_002119"
     CDS             complement(16006..16695)
                     /locus_tag="OO470_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6251365.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(16789..18936)
                     /gene="fdhF"
                     /locus_tag="OO470_002120"
                     /pseudo
     CDS             complement(16789..18936)
                     /gene="fdhF"
                     /locus_tag="OO470_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(19134..20600)
                     /gene="mdtP"
                     /locus_tag="OO470_002121"
     CDS             complement(19134..20600)
                     /gene="mdtP"
                     /locus_tag="OO470_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6251366.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(20597..22645)
                     /gene="mdtO"
                     /locus_tag="OO470_002122"
     CDS             complement(20597..22645)
                     /gene="mdtO"
                     /locus_tag="OO470_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6251367.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            complement(22645..23676)
                     /gene="mdtN"
                     /locus_tag="OO470_002123"
     CDS             complement(22645..23676)
                     /gene="mdtN"
                     /locus_tag="OO470_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6251368.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
BASE COUNT         5370 a         6125 c         6409 g         5839 t
ORIGIN      
        1 cccgttttta ttttctccgc acattgtgtg agcttttgcg atattctgaa aaaatgagaa
       61 ttcaggcata acgtctttct cagatcataa aaaacatcgc aaacagggaa cgcatggaaa
      121 tcttcttcac catactgata atgaccctcg tggtctcgct gtccggggtg gtcactcgtg
      181 tcatgccctt tcagatcccg cttccgctta tgcaaatcgc catcggtgcg ctactggcgt
      241 ggccgacgtt tggtttgcat gtggagtttg atcctgaact ctttttagtc ttgtttatcc
      301 cgccgttgct gttcgctgat ggctggaaaa cgccgacccg tgaatttctc gaacatggtc
      361 gagagatttt cggcctcgcg ctggcgctgg tggtggtcac cgtggtcggc attggcttcc
      421 ttatttactg ggtggtgccg ggcattccgc tgatccccgc ctttgcgctg gcggcggtac
      481 tttctccgac cgatgctgtg gcgctctccg ggattgttgg cgaagggcgc atcccgaaaa
      541 aaatcatggg cattttgcag ggcgaagcgt tgatgaatga cgcttccggc ctggtgtcgt
      601 tgaagtttgc cgtggcagtg gcgatgggga cgatgatctt cactgtcggc ggtgcgacgg
      661 tcgaatttat gaaagtagcc attggcggta ttcttgccgg ttttgtggtg agctggctgt
      721 atggtcgctc gctgcgattc ctcagccgct ggggcggtga tgaacccgcg acgcagatcg
      781 ttctgctgtt cttgctgcca ttcgcttctt atctgattgc cgaacatatt ggcgtttcgg
      841 gcatcctcgc tgcggttgcc gccgggatga ccatcacccg ctccggtgtg atgcgccgtg
      901 cgccactggc aatgcgcctg cgcgcaaaca gcacctgggc gatgctggaa tttgtcttta
      961 acggcatggt gttcctgctg ttaggtctgc aactgccggg tattctggag acgtcgctga
     1021 tggcggcaga aatcgaccct aacgtcgaaa tctggatgct gtttaccgat attattctga
     1081 tatatgcggc gctgatgctg gtccgtttcg gctggctgtg gacgatgaaa aagttcagca
     1141 accgcttcct gaagaagaag ccgatggagt ttggttcgtg gaccacacga gaaatcctga
     1201 tcgcgtcttt cgctggggtg cgtggggcga tcactctggc cggtgtgctc tcaatcccgc
     1261 tgctcttgcc ggatggtaac gtcttcccgg cgcgctatga gctggtgttc ctggcggctg
     1321 gcgtcattct cttctcgctg tttgtcggcg tggtgatgtt gcctattctg ctacaacaca
     1381 ttgaagtcgc ggaccattcg caacaattga aagaagaacg tattgcgcga gcggcaacgg
     1441 cagaagtggc gattgtggcg atccagaaaa tggaggagcg tctggcggcg gataccgaag
     1501 agaatatcga taaccagttg cttacggagg tcagttctcg cgtcattggt aacctgcgtc
     1561 gtcgcgccga tggacgtaac gacgttgaaa gttccgtgca ggaagagaac cttgagcgtc
     1621 gcttccgtct ggcggcattg cgttctgaac gtgctgaact gtaccacctg cgcgccacgc
     1681 gggagatcag caacgaaacg ctgcaaaaat tactgcacga tctcgatttg cttgaagcgt
     1741 tgctaattga ggaaaatcag taagccgaaa gcgacgtaac caggcccgag gtaatctgaa
     1801 aaaggtgcaa tcttttgcac ctttaacagg ttagctcggg cgaacattca tgattctaaa
     1861 acaaataagt tatctctctt gtaaagtcaa ttcattggct gttcttcgac ttaacctcaa
     1921 tctgcgtgtt gcataatatc tgcagtaatc ttcatagcga ctgcattaat attatcctca
     1981 gtttgtgaca tgccagtatg agaggtacaa tcttcttcaa agaatttctg tagtgttacc
     2041 agggtatcac tatcaatggg gatattttcc tccccacgga gaatggaaat gcccattccc
     2101 tggacgctag cctcatcgaa attaacccga tagagacggg tattaaacat acaggctttt
     2161 gttaaaatag aacagttgag atggcagttg cttaaattgg cattattaaa attaacatta
     2221 gccatattag agccggtaca tataaccatt tcgaggaatg tatttgtcag ccacgtacaa
     2281 aagagtttgc tatcaattaa gtttatattc tggaggattg ctttgttcag attgacgcga
     2341 gacagatcag catgttcaaa gttaattaaa tcgagtttag agaaactaag gtcggcttta
     2401 aataaattaa catatggcgc tattacttta taaagattta cctcagccat gtacgccata
     2461 taaaaatttg aatagtcaag gaaggtattt ttaatattgg ctctattcat tgctgcattg
     2521 tagaattgaa catgcatagc ggagcatgca tttagagttc cgtcggccat ttttatctga
     2581 gcgaaatttg agtcatcgag aatacaatta tcaaaaagta ttttttccat tatggcgtaa
     2641 taaaaattcg ttttatacat attacagtcc tggaaaatag cgttttgtag tctgcaacct
     2701 gcaaaattcg aattggataa atcttcatct ttaatcacga tacccttcag gctaaggtgt
     2761 gaaaaatcgt aaccaccatt tactatctgt ggaatttcga tagtattatt atggacggcg
     2821 acattattta taaaatatac caccttaatg gatacatcct tttctttact aaggtaaact
     2881 cttctcgtaa ttctatcgtc acatttatta tttgcatata acgctatatc tataaaatat
     2941 ttatgtattt tcctgtccgt ttctttcgtg atttttcctg atggtaaact atcataaatt
     3001 cttttcattt tctgaatcaa gcctatcact ttttcatgat catctatttt gaaaagagag
     3061 agggctttat tgtcaattaa cgtgttttta tgatgtttgc tgtaattatc tgattttgtt
     3121 ttctttgtat gtgatgaact tgtgatagag tggttatcgt taatctggca aagagggaaa
     3181 aacatattat ttaaaccatt atagcgcata aaatatcctt ataattaaca agaaagaaaa
     3241 ggcatattct ctgcattatc attttctacg actgtcaaaa atcgctcatt ttttaatgag
     3301 tttatttgtt taatattatg ggaaaaggtg atgcatgttg gagaggaagc gtattccccg
     3361 gtcaggcgac cggggaaggg gagagattaa tgtgcgcggc cttgctcaac gccaaagccg
     3421 gtctgggagc ggataaactg cgcgcggaac agttcacgct cgcgcgcccc ttccgctgag
     3481 ttatcggttg ccgagaagaa ccagatgccg aggaatgcca cgctgataga gaacagcgcc
     3541 gggtattcat acgggaagat ggctttttcg tgaccgagga tctgtaccca aatagtcggg
     3601 ccgaggatca tcagcaccac tgcggtaatc agccccagcc agccgcccag catcgcgcca
     3661 cgggtagtca gtttcgacca gtacatggaa agcagaatga tcgggaagtt acagctcgcc
     3721 gcgatggcaa acgcaaggcc taccataaag gcgatgttct gattctcaaa cagcacgccg
     3781 aggataatcg caatcacgcc gaggatcagt acggtgattt ttgatacccg cagctcttca
     3841 cgttcggtcg cgcctttttt gaagacgtta gcgtacaagt catgcgaaac cgccgatgcg
     3901 cccgccagtg tcagatccgc aaccaccgcg aggatagtgg cgaaagcaac cgctgaaata
     3961 aaaccgagga ataggttgcc gcccactgca ttcgccaggt gaacggccgc catgttgtta
     4021 ccaccaatca gatggcccgc cgcgtcttta tattccggat tcgcaccaac aagcatgatc
     4081 gcgccgaagc cgataataaa ggtcagaata tagaagtagc ccataaatcc ggtggcgtag
     4141 aacacgctct tacgtgcttc gcgggcatcg ctgactgtaa agaagcgcat cagaatgtgc
     4201 ggcaagcccg ccgtaccaaa catcagtccc agccccagag agagcgcgga gatcggatct
     4261 ttcaccagtc cgcccggttt catgatgtcg acacctttcg ggtgtaccgc catcgcttcg
     4321 ctgaacagat tgttgaagct aaagccgacg tgtttcatca ccataaaggc cataaagctg
     4381 gcaccgaaca gcaacagcac agctttgata atttgtaccc aggtggtcgc cagcatgccg
     4441 ccgaacagga cgtacatcat catcagcacg ccgaccagca ccaccgcaat gtgatagtta
     4501 aggccaaaca gcagctcgat cagtttacct gcgcctacca tctgagcgat aaggtaaagc
     4561 gccaccacta ccagagaacc acaggccgaa agaatacgaa tcggcccttg tttcagacga
     4621 taagaggcca catcggcaga ggtgtagcgt cccaggttac gcagacgttc ggcaatcaga
     4681 aacagaatga ttggccagcc caccaggaag cccaacgagt agatcagccc gtcatagccg
     4741 gaggtaaaca ccagcgcgga gatccccaag aatgaggcgg cggacatata gtccccggca
     4801 atcgccagcc cgttctggaa gccagtgata ttgccgcctg cggtgtagta gtcgctacga
     4861 gaacgtacgc gttttgatgc ccagtaggta atgccgagcg taaacacgac gaaaatcagg
     4921 aacataataa tcgcctgcca gttcgttggc tggcgctcta cggccccgct aatagcatcc
     4981 gcggcgttag ctgcgaaagg gagtgtggcg gcaagcgccg tcagaactct tttcatgatg
     5041 cttgtacctc atgcaggact tcattattaa gacggtcgaa ttcgccgttc gcccgccaga
     5101 tgtagatacc ggtgagaaca aaggagatca caatcactcc aacaccaatt ggaataccgc
     5161 gtgtgacgct ggtgttcgga ttcagcggcg tgcccagcca gccgggcgcg aaggcgatca
     5221 gtaaaataaa gccgatataa actgccagca taataatcga caggatggtg gcaaaccgtt
     5281 gccgtttttc gactaactcc ctgaaatgcg cattgtcttc tatccgctga taaatcgtac
     5341 cattcatcac agattctcca gaggtaatgt agggattgtt ttaattcccg ctcccttatg
     5401 ggagaaggtt aacgctcggg taacccttgc cgaatgtagg ccggataagg cgtttacgcc
     5461 gcatccggca atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acaaaccgtt
     5521 accgattcgc atccggcaat tgtgggttac gatggcatcg cgatagcctg cttctcttca
     5581 agcagcttct cgaccacgcc aggatcggca agcgtcgagg tatcgcccag gttgctggta
     5641 tcgcccgccg caattttgcg cagaatacgg cgcataattt tgccggagcg ggttttaggc
     5701 agggagtcgg tccagtgcag cacgtctggc gtcgccagcg ggccaatctc tttacgcacc
     5761 cagttgcgga cttctgcgta cagttctggt gacggttcct ccccgtgatt aagcgtgaca
     5821 taggcgtaga tcgcctgacc tttaatattg tgcggaatac ctactacggc ggcttcggca
     5881 atcttcggat gcgccaccag cgccgactct atctctgccg tccccagacg gtgaccggag
     5941 acgttcagca cgtcgtccac acgcccggtg atccagtaat agccatcttc atcgcgacgc
     6001 gcgccgtcgc cgctgaaata catatttttg aaggtggaaa aataggtctg ctcaaaacgt
     6061 tcgtgatcgc caaacagcgt acgcgcctga ccaggccagg agtcggtgat caccaggcta
     6121 ccttcggtag ccccttccag cgggttacct tcgttatcga ccagcgccgg ttgcacgccg
     6181 aagaacggac gtgttgccga accggctttc agctcggtag cgccaggcag cggggtgatc
     6241 atgaaaccgc cggtttcggt ctgccaccag gtatcgacca ccggacattt ctcgttgccg
     6301 atttttttcc agtaccactc ccacgcttcc gggttaattg gctcgcccac ggaaccgaga
     6361 atgcgtagcg acgaacggtc ggtgccttca atcgctttat cgccttccgc catcagcgcg
     6421 cggatcgccg tgggtgcggt atagagaata ttgacctgat gcttgtccac cacctgcgcc
     6481 atacgggcag gcgtcggcca gttcggtacg ccttcaaaca tcagcgtggt cgcgccgcag
     6541 gccagcgggc cgtacagcaa ataactgtgt ccggtaaccc agcccacatc ggcggtgcac
     6601 cagtagatat cacccggatg ataatcaaag acatatttaa aggtcagcgc cgcgtacacc
     6661 agataaccgc cggtagtgtg cagtacgcct tttggttttc cggtagaacc ggaggtatag
     6721 agaataaaca gcggatcttc ggcgttcatc tcttccgcct ggtgctgatc gctggcttgc
     6781 tcaaccaggt cgtgccacca caggtcgcgc ccttcctgcc agtcgatgtt tccgccagta
     6841 cgcttcagta ccaccacatg ctctacgctg gtgacgttcg ggttttttag tgcgtcatca
     6901 acgtttttct tcagcggaat actacgcccg gcgcgcacgc cttcgtcgga agtgatcacc
     6961 agtcgtgagt tggaatcaat aatgcgcccg gcaacggctt ccggcgagaa gccgccgaaa
     7021 atcaccgaat gcatcgcgcc aatgcgggcg caggccagca tcgcaaccgc ggcttccggc
     7081 accatcggca tataaatcgc caccacatca ccttttttaa tgcccagctc gagcagggta
     7141 ttggcgaagc ggcagacgtc gcggtgcagc tctttatagc tgatatgttt gctctggctg
     7201 gcgtcgtcgc cttcccagat gatggcggtg cgatcgccgt tttcttgcag atggcggtca
     7261 aggcagtttg ccgccagatt cagcgtgccg tcctcgtacc atttaatgga cacattaccg
     7321 ggggcaaagg aggtattttt caccttctgg tacggtttga tccagtcgag aatttttccc
     7381 tgttcgcccc agaaggtatc aggtacgtta atagattgtt gatacatcgc ctcgtactgc
     7441 tgagggttta tcaggcaacg gtctgcgatg ttggcaggaa tggtgtgttt gtgaatttgg
     7501 ctcatgcttt tgttctcctt gtaggatgtt aataatatgt ggcataagcg ttaaatgtag
     7561 gggtattggc agttttgttt agtatttggg cgacagatca cgcaaaagta gaatggtgca
     7621 aataaacggc agggtaattt ttgaaggtca ccaacaaaag ttgattaatt ctttgaggaa
     7681 catgcagtta tgcatgctgt tgaaaaagag gaagatactg actaactcta aagtggtatt
     7741 tcacatgcac ttacaattga ttaaagacaa cattcacagt gtggttattt gttacacata
     7801 ggggtgagca atgtcatgac agtgtaggtg cggttactgt cgtgaaaaac aataaaaacc
     7861 gccattgcaa caatggcgca attcggatga agcccctatg acaaggataa aaataaacgc
     7921 acgccgtatc ttcagcttat tgattccttt tttctttttc acttctgttc acgctgaaca
     7981 aacggcagct cccgcaaaac ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca
     8041 gcatcacgat caatatctct cctggaaagc cacctcggaa cagtcagagc gtgttgacgc
     8101 cctggcggaa gatccacggc tggtgatcct gtgggcgggg tatcccttct cgcgcgatta
     8161 caacaagcct cgtggacatg cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg
     8221 tgcgccgaaa aacgctgaag atggtccgct accgatggcg tgctggagtt gtaaaagccc
     8281 ggatgtggcg cgtctgatcc agaaagacgg cgaagatggc tacttccacg gtaaatgggc
     8341 gcgcggcggc ccggaaatcg tcaacaactt aggttgtgcc gactgccata acaccgcctc
     8401 accagagttc gccaaaggca aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc
     8461 gatggaagcc attggtaaac cttttgagaa agccggacgt ttcgaccagc aatcgatggt
     8521 ttgcggtcag tgccatgtgg agtattactt cgacggcaaa aacaaagcgg ttaaattccc
     8581 gtgggatgac ggcatgaaag tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc
     8641 tgactggact aactccctgt cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga
     8701 aacctggaca gcgggcattc acggtaaaaa caacgtgacc tgtatcgact gccatatgcc
     8761 aaaagtgcag aacgccgaag gcaaactcta caccgaccat aaaattggta atccgtttga
     8821 taacttcgcc cagacttgtg cgaactgcca tacccaggat aaagctgcct tgcaaaaagt
     8881 ggtcgcggaa cgtaagcaat cgattaacga cctgaaaatc aaggttgaag atcaactggt
     8941 tcacgctcac ttcgaagcga aagcggcgct ggatgcaggc gcgacggaag ccgaaatgaa
     9001 gccgattcag gacgatatcc gtcatgccca gtggcgttgg gatctggcga tcgcttccca
     9061 cggtattcat atgcacgcac cggaagaagg tctacggatg ctcggtacgg cgatggataa
     9121 agcggcggat gcgcgcacca aactggcgcg cctgctggcg accaaaggta tcacccatga
     9181 aatccagatc ccggatatct cgaccaaaga gaaagcccag caggccattg gcctgaacat
     9241 ggaacaaatc aaggccgaga agcaggactt catcaaaacg gtgatcccgc agtgggaaga
     9301 gcaggcacgt aaaaacggtc tgttaagcca ataaccccgt tccgcctcgc aaggggcgga
     9361 aaacacaatg gagtgaatat gagcgtatta cgttcgttat taactgctgg ggtgctggcg
     9421 tcgggcctgt tgtggagcct gaacggaatt accgctaccc ctgcggtgca ggcatctggt
     9481 gatcgctacg aagttaccca gcagcgtaac ccggatgccg cctgtctgga ctgtcataaa
     9541 ccagataccg aaggtatgca tggcaaacat gcttccgtta tcaacccgaa taacaaattg
     9601 ccggtcacct gcaccaactg tcacggccaa gcatcaccgc aacaccgcga aggggtgaaa
     9661 gatgtgatgc gctttaacga gccgatgtat aaggtcggag agcagaacag cgtctgtatg
     9721 tcctgtcatc tgccggaaca gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc
     9781 aaagtggcgt gcgccagctg ccattccctg catccacagc aagatacgat gcagacgtta
     9841 agtgacaaag gacggattaa gatttgcgtc gattgccaca gcgatcagcg caccaatccg
     9901 aactttaacc cggcgtctgt tccgttgctt aaggagcagc catgacctgg tctcgtcgcc
     9961 agtttctcac cggcgtcggc gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg
    10021 cgaaaacgtt gaatatcaat ggggtgcgtt acggcatggt gcatgacgag tcgttatgca
    10081 tcggctgtac cgcctgtatg gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct
    10141 cgcgcctgac gattattcgt agcgagccgc agggcgaatt tcctgatgtg aaatatcgct
    10201 tcttccgtaa gtcttgccag cactgcgatc atgtgccgtg cgttgacgtc tgcccgaccg
    10261 gtgcgtcttt tcgtgatgcc gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg
    10321 gttgtcagta ctgcatcgcc gcctgtccgt accgcgtgcg ctttatccat ccggtcacga
    10381 agacggcgga caaatgcgat ttctgtcgta agaccaattt gcaggccggt aagcttcccg
    10441 cgtgcgttga agcttgcccg acgaaggcgc tgacgtttgg caatctggac gatcctaaca
    10501 gtgagatttc gcaactgctg cgccagaagc ctacttaccg ctacaagctg gcgctgggaa
    10561 ccaaaccgaa gctgtaccgc gtaccgttta aatacgggga ggtgagccaa tgacgcagac
    10621 ttccgcattt cattttgaat cgctggtgtg ggactggccg attgccatct acctgttttt
    10681 gattggtatt tctgccggtc tggtgacgct ggccgtgctg ttacgtcgct tctacccgca
    10741 ggcgggcggt gcagacagta cgttgctgcg caccacgctg attgtcgggc cgggcgcggt
    10801 gatcctcggt ctgttgatcc tcgtcttcca cctgacaaga ccgtggacct tctggaagct
    10861 gatgttccac tacagtttta cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta
    10921 catggtggtg ctggtgctgt ggctggcgaa aatctttgaa catgatttgc ttgccctgca
    10981 acaacgctgg ttgccgaagc tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt
    11041 tcatcgcgga ctggaaacgc tgatgctggt gttggcggtg ctgctggggg cttataccgg
    11101 ctttctgctg tcggcgctga aatcgtatcc gttcctcaat aacccgagcc tgccggtgct
    11161 gctcctcttc tccggcatct cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg
    11221 ccaacgcagt aacccgcatt ccacggaagc gcactttgtt caccggatgg aaatccccgt
    11281 ggtatggggt gaaatcttcc tgctggtggc gttttttgtc ggtctggcgc tgggcgatga
    11341 cggtaaagtt cgtgcgctgg tggcggcatt aggtggcggt ttctggacgt ggtggttctg
    11401 gcttggtgtc gccgggctgg ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg
    11461 cagttccggc attcctgccg tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat
    11521 gctgcgcttt ttcattctct acgccggaca gttgacggtg gcgtaagcca gaaaagaggt
    11581 ggtttctgga cgtattcctt cctgaagtcg gttttctggc gttgttgtta agtctcgggg
    11641 tcaacgtgtt gaccccgttg acggcctttg cgggagtgcg gttgcgctgg cctgccatga
    11701 tgcgactcac ttgcatcggc attctggcgc agttcgcgct cctgctgctc gcctttggcg
    11761 tactgacgta ttgttttctc atcagcgatt tctcggtcat ttatgtcgcc caacatagct
    11821 acagcctgct gtcgtgggaa ctcaaactgg cggcggtgtg gggcggtcat gaaggttcgc
    11881 tgctgctttg ggtgctgctg ctttccgcct ggagcgcgct gtttgcctgg cattatcggc
    11941 agcaaagcga tccgctattt ccgctgacgc tagccgtttt atctctcatg ctcgccgcac
    12001 tgctactgtt tgtggtgctg tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg
    12061 aaggccgcga tctcaatccg atgctgcaac atcccggtct tatctttcat ccaccgctgc
    12121 tttgccttgg ctatggcggt ttgatggtag cggcgagcgt ggcgctggcg agtctactgc
    12181 gcggcgagtt tgatgcggcc agcgcccgaa tttgctggcg ctgggcgtta cctggctgga
    12241 gtgcattaac ggcggggatc atcctcggtt cctggtgggc ctactgcgaa ctcggctggg
    12301 gcggctggtg gttctgggat ccggtggaaa acgcctcttt attaccctgg ctttctgcca
    12361 ctgcgctgct gcacagtttg tccctgacac gccagcgggg gattttccgc cactggtcgc
    12421 tgttgctggc gatagttact ctgatgctgt cgctgctggg caccttaatt gtccgttctg
    12481 gcattctggt ttcggttcat gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca
    12541 gcctgtttgc actgattagc cttgcgtctt tggctctgta tggctggcga gcgcgggacg
    12601 gtggcccggc ggtgcgtttt tcggggttat cgcgggaaat gttaatcctc gctacgctgt
    12661 tgctgttttg cgcagtgcta ctgatcgtgc tggtgggaac gctttatccg atgatttacg
    12721 gcctgctggg ctggggacgc ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac
    12781 cgtttggtct gttgatgctg gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg
    12841 tgcagcttcc ggcgctggta gctcatgctg gcgtgctgtt atttgccgcg gggatcgtgg
    12901 tttccagtgt cagtcgtcag gagatcagcc tgaatttaca gccgggtcag caggtgacgc
    12961 tggcaggata caccttccgt tttgagcgac tcgatctgca agccagaggc aattacacca
    13021 gcgaaaaagc gatagtggcg ctgtttgacc atcagcaacg cattggtgaa ttaacgccgg
    13081 agcggcgttt ttatgaagca cgccgtcagc aaatgatgga accgtcaatt cgctggaacg
    13141 gcatccatga ctggtatgcg gtcatgggtg aaaaaaccag agcggatcgt tacgcttttc
    13201 gtttgtatgt acaaagcggt gtgcgctgga tctggggggg aggattgttg atgattgcgg
    13261 gcgcattgtt aagcggatgg cgggggaaga agcgcgatga ataaagggct tctcacgcta
    13321 ctgctgttat ttacctgttt tgcgcgcgct caggttgtag acacctggca attcgccaat
    13381 ccgcaacaac agcaacaggc gttaaatatt gccagccagt tacgttgtcc gcagtgccag
    13441 aatcaaaact tactcgaatc caatgcgccg gtggcggtca gtatgcgcca tcaggtttat
    13501 agcatggtgt cggaggggaa gaacgaagtc gaaatcattg gctggatgac cgaacgctac
    13561 ggagattttg ttcgctataa cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg
    13621 ccagtggtgt tgttattgct gatggcactg atcctctggc gagtgagggc taagcgatga
    13681 aacagcctca aataccggtg aaaatgctga caacactcac gatattgatg gtattcctct
    13741 gtgtcggcag ttatctgtta agcccaaaat ggcaggcggt acgtgcggag tatcagcgtc
    13801 agcgcgatcc gctacatcag tttgccagcc agcaaacccc ggaagcgcag cttcaggcat
    13861 tgcaggataa aatccgcgct aatccacaaa acagcgaaca atgggcgtta ctgggcgagt
    13921 actatctgtg gcaaaacgat tacagcaatt cgctgctggc gtatcgtcag gcgttgcaac
    13981 tacgtggtga gaacgctgaa ctgtatgcgg cgctggcgac ggtgctttat taccaggcca
    14041 gccagcatat gaccgcccag actcgcgcaa tgatcgacaa agccctcgcg ctggactcta
    14101 acgaaatcac cgctctgatg ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc
    14161 aagccatcga actatggcaa aaagtgatgg atctcaactc accccggatc aaccggacac
    14221 agctggtcga gtcgattaat atggcaaaac tgttgcagcg gagatcggat taatcatctg
    14281 gtgatgaaga ttatgtcatt gagctgcata aaaaataatc gaatgaacat atgccaaaaa
    14341 taatcaccaa tcagtattat tgcagattaa acaaataaaa atctttccat aataaatggt
    14401 tattcattaa tcctgccata aactcgcgtt attatgcatt aatgcagcga aaagctctgt
    14461 tgttaaaggg ttgcgcaaca taccgcgcaa atgatactga tcataagcgt taaaaaaatc
    14521 tacaaaccaa cgcaacacaa ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa
    14581 cggggaacgg cgctccattg aggaagttat tctgatgaaa aatataaaat tcagcctggc
    14641 ctggcagatt ctgtttgcta tggtgctggg cattctcctg ggaagctacc tgcactacca
    14701 tagcgacagc cgcgactggc tggtcgtcaa tttgctctct ccggcgggtg atatcttcat
    14761 ccatctgatc aaaatgattg ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc
    14821 gggtgttggt gatgccaaac agctcgggcg tattggcgcg aaaaccatta tttacttcga
    14881 ggtgatcacc accgtcgcca tcattttggg gatcactctg gcgaacgtct tccagcccgg
    14941 tgccggggtg gatatgtcgc agctggcgac cgtcgatatc tcgaaatatc agagcactac
    15001 ggaagcggta caaagcagtt cccacggcat tatgggcacg attttgtcgc tggtgccgac
    15061 gaacattgtg gcgtcgatgg cgaaaggcga aatgctgccg atcatctttt tctcggtgct
    15121 gtttggtctg gggctttctt ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt
    15181 ccgctccatc tctgaaacca tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt
    15241 gggtgtgttt gcgctgattg cggtgacggt ggctaacttt ggtttctcgt ctctgtggcc
    15301 actggcgaaa ctggtgctgc tggtgcattt cgccattctg ttcttcgcgc tggtagtgct
    15361 gggaattgtg gcacgtctgt gcgggttaag cgtctggatc ctgattcgta ttctgaaaga
    15421 tgagctgatt ctggcgtact ccactgccag ctctgaaagc gtactgccgc gaattattga
    15481 gaagatggaa gcctacggag caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta
    15541 ctcttttaac cttgatggtt cgacgctgta tcaaagtatt gccgctattt tcatcgctca
    15601 gctgtatggc attgacctgt ccatctggca ggaaatcatt ctggtgctga cgctgatggt
    15661 gacctcgaaa gggattgctg gcgtggctgg cgtgtcgttt gtggtgttgc tggcaacgct
    15721 gggtagcgta ggtattccgc tggaaggtct ggcgtttatt gctggtgttg accgtatcct
    15781 cgacatggcg cgtactgcgc tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc
    15841 caagtgggaa cacaaatttg accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg
    15901 caaatttgat aaaactgcgg atcaataatt gaagattgcc ggggatatcc cccggcaatg
    15961 tgtggatgcc tgatgcgacg cttgccgcgt cttatcaggc ctaccttaac cgttggttaa
    16021 tttttcaaac tcttcacacc cggtatcaaa cccttccata cagctcaggt tcaaccagtg
    16081 cagcgccttt tgtttgttct tctcgataaa accttcctca ccgtttaaga acatcattcc
    16141 cgcccagtac tcggaataac cggttcggga aattgcagag ctgcgtttga aataccaggt
    16201 tgccttgtca tcgtcggcct taatgcccac gccgttggcg taaatcaaac caagcagcat
    16261 ttgggcatcg acggcggagt cattctccag atcttccgag gcgttttcta acagcgaaat
    16321 cgcctttgga taatccggtt tacccgcttg agtatttacc agaatatgcg ccagcgttac
    16381 ttcaccttct ttactgcccg cttgcgccgc tttctctgca agaacttttg cttgtggata
    16441 gtccagactg accggattgg tgattttgat ctgcgccaac agcgcgcagg catcagcatc
    16501 accgctgtcg gcggctttct gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa
    16561 ccagctgtcg gcaagaaaat attgtgcacg tcggtccccg gcctctgctg cctttaaata
    16621 ctggctgcca ggctcggagt cgttcgcgtg ggcaaagaat gtcaaaaaca acatcaatgc
    16681 gataattttt ttcattttga taatacgatt gggtagggag taaccagtat aaagagttcg
    16741 aatggataaa aaaacagcct ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc
    16801 cgcttcgcgc aggcgagttt tcaacttgtt gtactcgtca atcacgtact gctcggcggc
    16861 gcgctgatcg gagatcggct cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt
    16921 aatcgggctt aagctttcgg taaccagctc gttacaggca ccaatccacc actggtaggt
    16981 catgtaaatc gcccctttgt tcggacgatc gctgacctgc gcacgggtga taattttgcc
    17041 tttacgcgag tgcactcaaa ccaatgcctc atcttcaata cccagacgtt tggcgtcttc
    17101 ggtattgatt tgtgcgtagc caggttcatc agccagcgcc gccagtgccg cacagttacc
    17161 ggtcatcgaa cggcaagagt agtgaccaac ttcacgcacc gttgacagta ccatcgggta
    17221 ctcgtcggtg agtttgtcga ttggcgctac ccagtcgcag gtgaagaact gcgccagacc
    17281 gttcggggta tcaaacttct ctttaaacag ataagaagtc ccctgatcgg catctgaagt
    17341 atcgcggcaa ggccactgaa tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta
    17401 gaaatccggg cacagatgac gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat
    17461 cggataaccc atacgggtgg cgatttcact gatgatttgc cagtccgttt tcagatccca
    17521 tttcggttca actgctttga agaaacgctg gaagccacgg tcagccgcag taaacacgcc
    17581 ttcatgctcg ccccacgacg ttgacggcaa aatgacatcc gccgccgacg cggttttggt
    17641 tataaagatg tcctgaacga tgaccagttc cagatcttca aaggctttac gtactgccga
    17701 cagctccgca tcagtttgca gcggatcttc gcccataatg tacgcggcac gcacttcgcc
    17761 atgcgctgcg cggtgcggca gctcgctgat gcgatagccg gtatgagcag gcaggctttc
    17821 cacgccccag gctttggcga atttttcgcg gttagccgga tctttcacgt actggtatcc
    17881 cggatacgta tccggcagcg cgcccatatc gcaggcaccc tgcacgttgt tctgaccacg
    17941 aaccgggttt acacccgcat gcggcttacc gaggttactg gtcagcatcg cgaggctggt
    18001 cagagaacgc acggtttcca cgccctggta gaactgggtt acacccatgc cccacaggat
    18061 ggcggcgctt ttcgcctggg catacatccg tgccgcctga cgaatctcac tggcgctgac
    18121 accggtgata tcttcaaccg actccggcgt gtagccttca acgattttac gatactcttc
    18181 aaagccttct gtacgggaag cgacgaacgc tttgtcgtac agattttctt caataatgac
    18241 atggcccatc gcattcaaca gcgcgatgtt cgagccgttt ttcagtgcaa tgtgcatgtc
    18301 agcaatgcgc gcggtttcaa ttttgcgcgg atcgcagacg ataattttcg ccccgttacg
    18361 tttagcgtta attacgtgat tcgccacgat tgggtgggaa tccgccgggt tgtacccgaa
    18421 aacgaacact aaatcggtat tatcaatttc gttaatagca ttgctcattg cgccattacc
    18481 gaccgattgg tgcagacctg caaccgatgg gccgtgtcag acacgagcgc agcagtcaac
    18541 gttattggta ccaataacgg cgcgcgcaaa tttttgcatt acatagttgg tttcgttacc
    18601 cgtaccacgc gaggagccgg tcgtctggat ggcatccgga ccgtacttct ctttgatggc
    18661 gctcaggcgc tcggcaacgt aattcagtgc ctcatctcag gaaacaggtt cgagtttgcc
    18721 gccacgctgg cgacggatca tgggggtttt caggcgcggg gtcaggatct gggtatcgtt
    18781 aatgaaatcc cagccataat aacccttcag acacagggta ccctggttgg ttttcccctg
    18841 cgctgcctcc gcccggacga ttttgccgtt atcgacgacc aggttgattt tgcaacctga
    18901 tgcgcaatag gggcaaaccg tgacgacttt tttcatcggt ctcgctccag ttaatcaaat
    18961 cacgcatacg cgctctcgac tacagtatgc atcttttatg ccacatttta tttggggtca
    19021 ttccctgata ttacgggcac tatttattca aaactctgac gaaaaacagg ctgtcgtcag
    19081 ttttgacgtg acgaaacgaa ataccgcgtg acagccatca cgcggcagac attttatttt
    19141 ttctcgacga cgggagccgc ctgatacccg ccgcccagcg atttcatcaa ctgaatgctt
    19201 tggatcaccc ggcggctgtc cagcatcagt aatgacatct cttcggcaag tactggcaac
    19261 cgggcttcag tggcctgtaa gcggctggtt aagccgcgct gataggcggc ctcggcagcg
    19321 cgctgggtga agcgcgttgc ttccacgtgt tcagcctgca tttctcgctc gtcgttgagc
    19381 gtttgcagac gcgtgccgtt gacggcaacg tcacgcaccg cgttcagtac tgactggttg
    19441 taacgttcaa tcatcatgtt gctggcggcg cgcgtgcctt cgagattggc attcaaccgt
    19501 ccaccgtcaa acagcggcaa tttcagaccc gggatgaagt tgaactggcg actggttttt
    19561 ttgaataagg tatccaggtg gatggcatca aggccgaaaa acgctttgat atcaaagctc
    19621 ggatagaaca acgcccgcgc ggaatccacc tgatctaatg acgcctgaac ataccagcgc
    19681 atggcttgca gatccgggcg tctggcgagc aactcataag agagtgtcgc cggaatgccg
    19741 gtctggactc gtggtaatgc cacaggtttg atctccggca tatcgctggc tcccgcgcca
    19801 atcaatgcgc gcagagattc tctcgtttcg gtgatttgcc ctttgacggc agcaatttgt
    19861 ttatcgaccg ccagaatctg tgcccgcgcg ccgtggaaag gcacttgtgc ttccagaccg
    19921 tgcgccactt tactttggtg cgctttcacc gcgtaatcaa tcacatcgcg agtttgttct
    19981 aacagatcga gcatctgata gctggcctgc atactgtaat aaagctgcgc tacgcccgtg
    20041 gtcagcgata gctctactgc tgcggtttct gccagcgcgg cattatgcgc gccaatggcg
    20101 gcggcaaccg ctgagcgatg cacaccccac aaatcaagat ccagtccggc aaacaaacct
    20161 actgtggctt ccgtatagta cggcccgtcc atccccagtg cgggcgcatc catcgcataa
    20221 gggcttaaaa agccgttcgc cgagacacgt tggcggttaa gcatccctaa cgctgccacc
    20281 tgcaactgtg aaccggcatc taacaaatcg gcctgcgatt gcgctttttc ttcccgcagt
    20341 ttcgcttcgg cgagggtgtg tgaaccactt agcgtccgtt ggatcagggc atccaactgc
    20401 gggtcattga gttgtttcca ccactgcgcc tgcggccagc cggagctggc aagatgaata
    20461 tcatcggcca gtttgatttg ttccggtttg agctgttgat gaggcgcaga atccttacgt
    20521 accagggcac agccggaaat cagcgtcgtg ctgccgagaa tgctgcacaa cagcagacgt
    20581 gaaagttgac gattgatcat tgcgtggctc cttgtgcctg ttccgtggcg ggcgttaatg
    20641 ccggggctgt ccagtctggc aagcgggcga aaagctggca gacgtgttgc tcctgtttta
    20701 ataaggtggg caggatggcc tgcgaggtgg gtgtctcttc aagcgttatt tgaggagaac
    20761 ggctgagtgc ggttgccaga ccggcagcgt atttctccag ggcgtcggca aactgacctg
    20821 ctctgtccgg ctgtagtgcg ttatccagcg cctgcgccga attccaggtg gcatcccagg
    20881 cgtgaaggat atcgcggccc tgacgaataa ccgtttgcga acgttcaatc agtaaggcgc
    20941 gttcttcgct gtccagttga cgctccagcg ccacgcgttg gcacatctct tcacaggcat
    21001 taaacgccgc atgcagaccg atacgaattt gcagataagt gcgcagagcc gtgacttcct
    21061 gctggcgtgg gatccgcatt actttactta acatacccag cgcgccagcc agtttttgcg
    21121 gcagcgtgcg cgcttcactt tcaggccaga caaaggtgta aatcatcgcg gacaccacgg
    21181 taccaatgat gatacccagg gcgcgatcgc gaatttccac caggtcgtac actgggccaa
    21241 aaacgttttc gagcgtggcg agcgcgaagg tgaccaccat ctgtgtgccg atataagaag
    21301 agcgttcaga gctggtggca atccatgcgg ccaacaggaa aatcggtgcc agcacaaaca
    21361 gcaattcgac aatattgtcc agccagggca tgaccagtag cgtgaataac agcgccagaa
    21421 tcgcgccgca aaaggccccg ccaaaacgca gcaccatctt ctggtacgac gaaccgacat
    21481 ttggattagc gacgatcacg caggtcagca tacaggtgtg aatgccttcc cagtccacgc
    21541 cgctgtaaaa ggtgtaacag atcaaacagg cgagcaatgt ttttaccgcg tagcgtatat
    21601 agtctggatt ggtaaaagca tcggcgacca ttgatggcgg tttggcggcg ggcgttggcg
    21661 gcgtattcgg gttcatctgg cccagttgta acaacgtctg gcagatattc tccaggttac
    21721 attcccgtgc cgccaccgct tcactttcac tgatccgcca gtcgctttgc cagcactgac
    21781 cttcagcaac ggcatgctgc agcttgttga tttctgaagc taatttttgc cgaaattcaa
    21841 taattgcctg agaatcagca aaagaggtgg gatcgtagcg attcagcgtc gagtaaatgt
    21901 aggttaccgt tgccacgcag ctttgccacc atgcgctttg agttcgccag ttggcatcgt
    21961 ccgcgagaca aaagacattc agtttttgca gcgccagcgc ctctctttca atccgcgttt
    22021 cgggtagcgg tgcgaggctg tccgtcaggt ggctaatggc atcatcaagc cgatcattaa
    22081 gcgcttgatg catttgcgta atggcacgat tgggaaacca cagcacgccg attaacgtca
    22141 tcagcaaggt tggatagagg ccaacaacga tacaccacag cgttaagcgc acgactactt
    22201 ccggatagtc gagcatggcg gggaaggttt gcccgtaaat ggcgacagcg aaaaagacca
    22261 gtcccagccg atgggtgcgc atcaaaaaca tgcagcccat caggatcggc ccggcgatga
    22321 tcaatcggat caacggttcg ccgtatgacc atttgtagat caaaaacagg ctgccgatct
    22381 ccagcaccgt ggcaaccaca aacaagatcg ccacaaattt ggtgtaaaac gcgttcgact
    22441 gaataccgat accgtaaaac agcactgcca gcgataacgc cacaaaaggg atctcaaaag
    22501 tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacgatc tgcggcacgc
    22561 ggccgggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta
    22621 atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc
    22681 gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc
    22741 aacgcggacc cagttaatag aacgagacac ttttggcagg ccgcccagca ccaggccgcc
    22801 gtcatccggt agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct
    22861 gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc
    22921 gcggaagttg gcgatcacgt accagtgacg agtgtcgatg agggtaaaaa tagggcgcat
    22981 ggcagaagca aattgcccga cggaggtttt gagggaaatg atccggccat caaatggcgc
    23041 gcgaacggtc gtcatttcca gatgtagttt ggtcagggca atatccgctt cgaccgccgc
    23101 acgttgcgcc accagcgcat cgacgccgct gacggcgctg gcggctgact gcgcctgtaa
    23161 caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac ggtcaacgtc
    23221 ttctgctgag acaaaaccct ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc
    23281 ctgtttcgcg gcggcacggg ctttttctac cgtggcatta accgagtcgg caccaaactg
    23341 ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc
    23401 ttcggctttc gccaggttgg cttcgtacgg gcgcgggtcg atgcggaaca gcaaatcgcc
    23461 ctgtttcacc gcctggttgt cggtgaccgc cagttctaca atgcgaccgc tgacttccgg
    23521 caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg cactgtctac
    23581 gagccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca gagcagggaa
    23641 tttactgcga ggtgcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa
    23701 aaatgccagc cagaacagca tggcgtagag agcaaaaagg gcg
//
LOCUS       DAJTUN010000058        22246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTUN010000058 DAJTUN010000000
VERSION     DAJTUN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.058"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..434)
                     /locus_tag="OO470_002124"
     CDS             complement(<1..434)
                     /locus_tag="OO470_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6251369.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTG"
     gene            442..942
                     /gene="tnpA"
                     /locus_tag="OO470_002125"
     CDS             442..942
                     /gene="tnpA"
                     /locus_tag="OO470_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6251370.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(981..1328)
                     /gene="grxD"
                     /locus_tag="OO470_002126"
     CDS             complement(981..1328)
                     /gene="grxD"
                     /locus_tag="OO470_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6251371.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1663..2490
                     /gene="mepH"
                     /locus_tag="OO470_002127"
     CDS             1663..2490
                     /gene="mepH"
                     /locus_tag="OO470_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6251372.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2618..3199
                     /gene="sodB"
                     /locus_tag="OO470_002128"
     CDS             2618..3199
                     /gene="sodB"
                     /locus_tag="OO470_002128"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6251373.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3345..4514)
                     /locus_tag="OO470_002129"
     CDS             complement(3345..4514)
                     /locus_tag="OO470_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251374.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="OO470_002130"
     CDS             complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="OO470_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6251375.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5068..6093
                     /gene="purR"
                     /locus_tag="OO470_002131"
     CDS             5068..6093
                     /gene="purR"
                     /locus_tag="OO470_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6251376.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6090..7022)
                     /locus_tag="OO470_002132"
     CDS             complement(6090..7022)
                     /locus_tag="OO470_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6251377.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7135..8346
                     /locus_tag="OO470_002133"
     CDS             7135..8346
                     /locus_tag="OO470_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6251378.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8637..9785
                     /gene="cfa"
                     /locus_tag="OO470_002134"
     CDS             8637..9785
                     /gene="cfa"
                     /locus_tag="OO470_002134"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6251379.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="OO470_002135"
     CDS             complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="OO470_002135"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6251380.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10681..12054
                     /gene="mdtK"
                     /locus_tag="OO470_002136"
     CDS             10681..12054
                     /gene="mdtK"
                     /locus_tag="OO470_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6251381.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12095..13351)
                     /locus_tag="OO470_002137"
     CDS             complement(12095..13351)
                     /locus_tag="OO470_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6251382.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13659..13735
                     /locus_tag="OO470_002138"
     tRNA            13659..13735
                     /locus_tag="OO470_002138"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13693..13695,aa:Val,seq:gac)
     gene            13740..13816
                     /locus_tag="OO470_002139"
     tRNA            13740..13816
                     /locus_tag="OO470_002139"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13774..13776,aa:Val,seq:gac)
     gene            13924..14229
                     /locus_tag="OO470_002140"
     CDS             13924..14229
                     /locus_tag="OO470_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6251383.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14355..15959
                     /locus_tag="OO470_002141"
     CDS             14355..15959
                     /locus_tag="OO470_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6251384.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(15971..16783)
                     /locus_tag="OO470_002142"
     CDS             complement(15971..16783)
                     /locus_tag="OO470_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251385.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16787..17572)
                     /locus_tag="OO470_002143"
     CDS             complement(16787..17572)
                     /locus_tag="OO470_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6251386.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17569..18237)
                     /locus_tag="OO470_002144"
     CDS             complement(17569..18237)
                     /locus_tag="OO470_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6251387.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(18301..18939)
                     /locus_tag="OO470_002145"
     CDS             complement(18301..18939)
                     /locus_tag="OO470_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6251388.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18952..21053)
                     /locus_tag="OO470_002146"
                     /pseudo
     CDS             complement(18952..21053)
                     /locus_tag="OO470_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(21074..21700)
                     /locus_tag="OO470_002147"
     CDS             complement(21074..21700)
                     /locus_tag="OO470_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6251389.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(22156..>22246)
                     /locus_tag="OO470_002148"
     CDS             complement(22156..>22246)
                     /locus_tag="OO470_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2767 family protein"
                     /protein_id="HCR6251390.1"
                     /translation="LRTALANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5569 a         5746 c         5182 g         5749 t
ORIGIN      
        1 cccgtgacct gacggctatt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgtcctgc gggtagcgga atgcagcatt ctgtccccgc
      121 tttttgaatc ggggaaaagc agcccgcttc tggaagaagt ttttgtaagc ccgctcaagg
      181 tctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg
      241 gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg
      301 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg ctcctgttga ccacctggtc ttaactggaa tttaaatgcc
      421 tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg
      481 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac caaataccga
      541 cgccagattt ttgaccatga cacgacagaa aaactacgca cttacttttc aaatgtatgt
      601 gctgattttg cagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta
      661 attaactatc ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcatatcg
      721 aataggttac tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt
      781 ttgtggagtc ctggctattt tgccagtagc tgcgaaggtg cgccaatatc cgtcatccgc
      841 cagtacattg aacaacagca aacacctggt caggtggaaa accgcgcctt atatccccgc
      901 cctgaaggac gaggttttac gacgcaccgg ataaaggact gacggatgcc cgttcgcatc
      961 cgtcagtatt gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca
     1021 gtttctttga tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa
     1081 ccgccgacca gctcgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc
     1141 ggcagttccg cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca
     1201 catgcggcaa gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa
     1261 cctttcatgt acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg
     1321 gtgctcattg tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt
     1381 cagtgcggca tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct
     1441 atttgttgca tttcaaatat tcgtttcttc tctgatgcgc ggcaaactgg cgtatttata
     1501 accattttta gctttcactg ctgcattttt ttgcgctcgc caacgaaacg tattttttaa
     1561 caataaaagc tattaacttt ctcttcttct atgcattaga atcatcaagt tttgtaaatc
     1621 agacgcaggc atgatagacc tgccttaaca gagggacgct cagtggcgcg gataaaccgt
     1681 atttcgatca cgctctgtgc tttacttttt accaccctgc ctttaacgcc tatggcccat
     1741 gcttcaaagc aagccaggga gagttctgct accactcata tcaccaaaaa agcagataaa
     1801 aagaaaagca cggcaaccac caaaaagacc cagaaagcca gccagaaaac agcgaaaaaa
     1861 gccgccagta aaagtatgac caaaagcaaa accgcttctt ctgttaaaaa atcttccatt
     1921 accgcttcta aaaacgccaa aactcgcagc aaacacgccg tcaataaaac ggcctcagcc
     1981 agctttaccg aaaagtgtac caaacgtaag ggctataaat cgcattgtgt gaaagtcaaa
     2041 aatgccgcgt caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa agccacgaaa
     2101 gtggcaatga ataaactgat gcagcaaatt ggtaagccat atcgttgggg cggcagctca
     2161 ccgcataccg gttttgattg cagcggcctg gtttattacg cttataaaga tttggtgaaa
     2221 attcgtattc cgcgtacggc gaatgaaatg tatcacctgc gtgatgcagg acctatcgaa
     2281 cgtagtgagc tgaaaaacgg cgacctggtc tttttccgta ctcagggacg cggtacagcc
     2341 gatcatgtcg gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg cacaggtcag
     2401 gaaattcaaa tcacttctct cagtgaagac tactggcagc gccactatgt tggcgctcgt
     2461 cgggtaatga ccccaaaaac acttcgctaa aactttaccc tgttgttacg gcaacagggt
     2521 aagttcatct tttgtctcac cttttaattt gctaccctat ccatacgcac aataaggcta
     2581 ttgtacgtat gcaaattaat aataaaggag agtagcaatg tcattcgaat tacctgcact
     2641 accatatgct aaagatgctc tggcaccgca catttctgcg gaaactatcg agtatcacta
     2701 cggcaagcat catcagactt atgtcactaa cctgaacaac ctgattaaag gtaccgcgtt
     2761 tgaaggtaaa tcactggaag agattattcg cagctctgaa ggtggcgtat tcaacaacgc
     2821 agctcaggtc tggaaccata ctttctactg gaactgcctg gcaccgaacg ccggtggcga
     2881 accgactgga aaagtcgctg aagctatcgc cgcatctttt ggcagctttg ccgatttcaa
     2941 agcgcagttt actgatgcag cgatcaaaaa ctttggttct ggctggacct ggctggtgaa
     3001 aaacagcgat ggcaaactgg ctatcgtttc aacctctaac gcgggtactc cgctgaccac
     3061 cgatgcgact ccgctgctga ccgttgatgt ctgggaacac gcttattaca tcgactatcg
     3121 caatgcacgt cctggctatc tggagcactt ctgggcgctg gtgaactggg aattcgtagc
     3181 gaaaaatctc gctgcataat aactgatggc aaatgcagca ttgcctgatg cgcttcgctt
     3241 accatgccaa cgcggatgag gcgttaacag cgcattcggc aagataaatc gcacgttgtc
     3301 agcaactgta acgcagaagg ttatccttct gcgtttttgt ttaattagct gttagcaacg
     3361 caaactgttt caggttgttt tctggctgac ataaacacca gcaataatgc cagtcccgcg
     3421 acaatcgccc ccgtcaccgg cacaaagctg tatcccagcc cagcggaaat taccgcacca
     3481 ccagcagctg ctcccagcgc atttccaaga ttaaaggcac caatattgac tgatgaagac
     3541 agacctggcg cttcactggc gacatgcatc acgcgcatct gtaacggagg tacaaccgca
     3601 aaggttgctg cgccccacac caccatgcta atagctgcgc cgaactcatt gcgggccagg
     3661 aacgggattg ccagcataat caccatcagc agcaacaaaa agcctttcaa cgtgccgtta
     3721 actgaacgat ctgccagttt gccgccgaga tagttaccga tagagaatcc gacaccaatc
     3781 agcaccagca ttgccgtgac gaacaccggt gttgcgtggg taatactttg cagtaccgga
     3841 gagatatagg tgtagagggt aaacattgca ccagctccca gtaccgtcgt cagcaatgca
     3901 gacagcacct gcggacgcat taataccgcc agctcttttt tcacttcagg tcgtgcccct
     3961 gtaccacctt taggtaatga gaagaacaga cttaccattg aaatcactcc cagccccgcc
     4021 gttgccagaa atgacatccg ccagccgatg gtttcaccca accaggtcgc cgccggcacg
     4081 ccaccgatat ttgccagggt taaccccata aacatagtgg caactgcgct ggcctgttta
     4141 tgttttggca ccacgcttgc ggccacgact gaacccaaac caaaaaatgc tccgtgattc
     4201 aggctggtca aaatacgtga aagcatcagg gtcatataat ccggcgcgat ggcggaaaga
     4261 acgttgccta gcgtgaaaat tgccatcagg aaaatcaacg cactgcggcg ggcacgatga
     4321 gaaagtagaa gcgtcatcag cggcgcgccc accattacgc caactgcata ggcactgatt
     4381 aacattccgg cagcgggaat cgagacatcc acaccgcgcg caatgacggg caacaagccc
     4441 attggcgaga actccgttgt cccgatacca aacgcgccaa tcgccagcgc cagcaacgga
     4501 tagttaattt tcatgcctta tctccacctc ttcgcgtcat tacgcgatat tcattaaagt
     4561 ggcgaaagca tgacagcaat cacaaaaaaa tgaaaataac aaaaagagaa aacacttttg
     4621 ccattttgct aacaaacagg aaggagatgc gagggagaac gcgctccctc gagaggaaat
     4681 cagtgcagcg cggcagtcaa acccacggct acgatcaaac cgaggacgat aatcgttgtt
     4741 accagtgaaa atttaaggtc ggtgctcatc aagttttctc cttttttatt accacacaaa
     4801 aagtgatatt acgcattttt acacactgtg atgaaaaaat ctcccgtcat ttataatgat
     4861 aagtgttttt accacttccc cttttcgtca agatcggcca aaattccacg cttacactat
     4921 ttgcgtactg gccattgacc ccttcctgac gctccgtgtc gtttttccgg cgtaccgcaa
     4981 cacttttgtt gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgatt ttgcaggagc
     5041 tgaagttagg gtctggagtg aaatggaatg gcaacaataa aagatgtagc gaaacgagca
     5101 aacgtttcca ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt cgctgaagaa
     5161 acgcgcaacg ccgtgtgggc agcgattaaa gaattacact actcccctag cgcggtggcg
     5221 cgtagcctga aggttaacca caccaagtct atcggtttgc tggcgaccag cagcgaagcg
     5281 gcctattttg ccgagatcat tgaagcagtt gaaaaaaatt gcttccagaa aggttacacc
     5341 ctgattctgg gtaatgcgtg gaacaatctt gagaaacagc gggcttatct gtcgatgatg
     5401 gcgcaaaaac gcgtcgatgg tctgctggtg atgtgttctg agtacccaga gccgttgctg
     5461 gcgatgctgg aagagtatcg ccatatccca atggtggtga tggactgggg tgaagcaaaa
     5521 gctgacttca ccgatgcggt cattgataac gcgttcgaag gcggctacat ggccgggcgt
     5581 tatctgattg aacgcggtca ccgcgaaatc ggcgtcatcc ccggcccgct ggaacgtaac
     5641 accggcgcag gccgccttgc cggttttatg aaggcgatgg aagaagcgat gatcaaggtg
     5701 ccggaaagct ggattgtgca gggtgacttt gaacctgaat ccggttatcg cgccatgcag
     5761 caaatcctgt cgcagccgca tcgccctact gccgtcttct gtggtggcga tatcatggca
     5821 atgggcgcac tttgtgctgc tgatgaaatg ggcctgcgcg tcccgcagga tgtttcgctg
     5881 atcggttatg ataacgtgcg caacgcgcgc tattttacgc cggcgctgac cacgatccat
     5941 cagccaaaag attcgctggg tgaaacagcg ttcaacatgc tgttggatcg tatcgtcaac
     6001 aaacgtgaag aaccgcagtc cattgaagtg catccgcgtt tgattgaacg ccgctccgtg
     6061 gctgacggcc cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt ctcttccggc
     6121 tcccgtagcc actccttatt cagcgtctca ctatcgccga gatactcaag cagccaggtt
     6181 aacgcaggcg acatatcatt ttgctgccat gtcagacaac atgccgaatc cgggaagggg
     6241 ttttccagtt caagtgctac ccacttcccc tcattaagcc acggtttggc gaaatgtgtt
     6301 ggcaccatcc ctatgcataa tcctgccgag atacaggttg ccgatgattc ccagtcaggt
     6361 acgacaagtc ttttttggtt atccagcaac caggtaatac gtttaggtag cgttcgcgag
     6421 gtgtcttcgc gcaccaacga cggccagttg cgcaacgtat catcgctgaa cgggccatcc
     6481 atcaacgcca gcgggtggtg actggcaaca acgcaactcc agcttagcat ccccatatcc
     6541 cggaaggcat aacgaccgcc taccggaatc gcgcgtgttg cgccaattgc cagttccacg
     6601 cgcccgtcgg aaagcgcatc ccagacaccg ttgaacactt cctgaaagac aagaagttcg
     6661 acatcatcaa aatggcgata aaaatcaacg atcatctgcc gtgtacgttc tggcctgaca
     6721 atattatcca ctgcgatagc taactgaccg cgccagccgt tcgctatctg ctgacattgc
     6781 tggcgggtga tctgcatttt tttgacaaca gagcgccctt ctttgagaaa ccacgctcca
     6841 gcagcggtca gctccacatc acggtgccgt cgttcaaaga gcggtaccgc cagccactct
     6901 tccagctgac gcacggtata gctgaccgca gaaggaacac gatgcagctc ctgtgccgca
     6961 gcgctaaaac taccattacg cgctaccgca tcaacaactt cgagtgaata ttctgaccac
     7021 atagtctgcc tgcaaaattt ttgaaaccag tcatcaaata ttaccgtttc acaacactaa
     7081 tttcactccc tacactttgc ggcggtgttt aattgagaga tttagagaat atacatgcaa
     7141 cctgggaaaa gatttttagt ctggctggcg ggtttgagcg tactcggttt tctggcaacc
     7201 gatatgtatc tgcctgcttt cgccgccata caggccgacc tgcaaacgcc tgcgtctgct
     7261 gtcagtgcca gccttagtct gttccttgcc ggttttgccg cagcccagct tctgtggggg
     7321 ccactctccg accgttatgg tcgtaaaccg gtgttattaa tcggcctgac aatttttgcg
     7381 ttaggtagtc tggggatgct gtgggtagaa aacgccgcca cgctgctggt attgcgtttt
     7441 gtacaggctg tgggggtctg cgccgcggcg gttatctggc aagcattagt gacagattat
     7501 tatccttcac agaaagtcaa ccgtattttt gcgaccatca tgccgctggt gggtctgtct
     7561 ccggcactgg ctcccctgtt aggaagctgg ctgctggtcc atttttcctg gcaggcgatt
     7621 ttcgccaccc tgtttgccat taccgtggtg ctgattctgc ctattttctg gctcaaaccc
     7681 acgacgaagg cccgtaacaa tagtcaggat ggtctgacct ttaccgacct gctacgttct
     7741 aaaacctatc gcggcaacgt gctgatatac gcagcctgtt cagccagttt ttttgcatgg
     7801 ctgaccggtt caccgttcat ccttagtgaa atgggctaca gcccggcagt tattggttta
     7861 agttatgtcc cgcaaactat cgcgtttctg attggtggtt atggctgtcg cgccgcactg
     7921 cagaaatggc aaggcaagca gttattaccg tggttgctgg tgctgtttgc tgtcagcgtc
     7981 attgcgacct gggctgcggg cttcattagc catgtgtcgc tggtcgaaat cctgatccca
     8041 ttctgtgtga tggcgattgc caatggcgcg atctacccta ttgttgtcgc ccaggcgctg
     8101 cgtcccttcc cacatgcaac cggtcgcgcc gcagcgttgc agaacactct gcaactgggt
     8161 ctgtgcttcc tcgcaagtct ggtagtttcc tggcttatta gtatcagcac gccattgctc
     8221 accaccacca gcgtgatgtt atcaacagta gtgctggtcg cgctgggtta catgatgcaa
     8281 cgttgtgaag aagttgactg cccgaatcat ggcaatgccg aagtcgctca tagcgaatca
     8341 cactgatcta tatcgatata cttatactta ggctgctaac aaaattttgt tgtatgattg
     8401 aaattagcgg cctatactaa tttcgagttg ttaaagctac gataaatatt atgtttttac
     8461 ggggacagga tcgttcccga ctcactatgg atggtcattt cggcaagggt tcctcctttc
     8521 cctctgttct acgtcggatt atagactcgc ggttttttct gcgagatttc tcacaaagcc
     8581 caaaaagcgt ctacgctgtt ttaaggttct gatcaccgac cagtgatgga gaaactatga
     8641 gttcatcgtg tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt atcgccaacg
     8701 aattacttag ccgtgccggt atagccatta acggttctgc cccggcggat attcgtgtga
     8761 aaaaccccga tttttttaaa cgcgttctgc aggaaggctc tttggggtta ggcgaaagtt
     8821 atatggatgg ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa gtcttacgcg
     8881 caggtctcga gaaccaactc ccccatcatt tcaaagacac gctgcgtatt gccggcgctc
     8941 gtctcttcaa tctgcagagt aaaaaacgtg cctggatagt cggcaaggag cattacgatt
     9001 tgggtaatga cttgttcagc cgcatgcttg atcccttcat gcaatattcc tgcgcttact
     9061 ggaaagatgc cgataatctg gaatctgccc agcaggcgaa gctcaaaatg atttgtgaaa
     9121 aattgcagtt aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg ggcggactag
     9181 cgcactacat ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt tctgccgaac
     9241 agcaaaaaat ggctcaggaa cgctgtaaag gcctggatgt caccattttg ctgcaagatt
     9301 atcgtgacct gaacgaccag tttgatcgta ttgtttctgt ggggatgttc gagcacgtcg
     9361 gaccgaaaaa ttacgatacc tattttgcgg tggtggatcg taatttgaaa ccggaaggta
     9421 tattcctgct ccatactatc ggttcgaaaa aaaccgatct gaatgttgat ccctggatta
     9481 ataaatatat ttttccgaac ggttgcctgc cctctgtacg ccagattgct caatccagcg
     9541 aaccccactt tgtgatggaa gactggcata acttcggtgc tgattacgat actacgttga
     9601 tggcgtggta tgaacgattc ctcgccgcat ggccagaaat tgcggataac tacagtgaac
     9661 gctttaaacg gatgtttacc tattatctga atgcctgcgc aggtgctttc cgcgcccgtg
     9721 atattcagct ctggcaggtg gtgttctcac gcggtgttga aaacggcctt cgagtcgctc
     9781 gctaaaggct attctatcgc cccctcttcg ggggcgagtt cagatcaggc ttctgtacct
     9841 ggttgattca tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc taccactgcc
     9901 tgagtttgtg gatcgatttc aatgttgacg cgtgcgccaa gttttttctt cccaagagtc
     9961 gtgcgttcca gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt gacttcgccg
    10021 acggtcaggc taataccgtc gatgccaata aatcctttgt acaggatata tttcatcaac
    10081 tgactatcct ggactttaaa ccagatctgg cgattatttt ccgaggttaa tattttcgcc
    10141 acttcagcgg tggtcataat atgacctgac attaagtgtc cgccaatttc atcactgaat
    10201 ttcgccgcac gctcaacgtt tacccaatcc cccactttta aatcgccaag attggtaatg
    10261 cgtaacgttt ctttcatcag gtcaaaactg acatggttgc cgttaatttc cgtcacggtc
    10321 aggcagcaac cgttatgcgc cacggaagca ccggtttcca ggccgtccag catgtggtcg
    10381 ggtaactcca ccacatgcgt acgaaaattt ggtttctcgt caatcgacac cagttttgcg
    10441 gtgccctgta caatccccgt aaacatactt acaactcctg aaatcagtta agacattctg
    10501 ttcagcacaa tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa tggctatttt
    10561 ttcactggag aattaataaa tcctcgctac aatagactga atttcccctg cttcttcttt
    10621 ttgctgccca ttcaggcggc tttttagtct ctcatataac tacaaataaa aggtgttcac
    10681 gtgcagaagt atatcagtga agcgcgtctg ttattagcat tagcaatccc ggtgattctc
    10741 gcgcaaatcg cccaaactgc gatgggtttt gtcgataccg tgatggcggg cggctatagt
    10801 gccaccgaca tggcggcggt cgctatcggt acttctatct ggcttccggc gatcctcttt
    10861 ggtcacggac tgctgctggc attaacgccg gttatcgcgc aattaaatgg ttccggtcga
    10921 cgtgagcgca ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt tgtttccgtt
    10981 ctcattatgc tggtgctgtg gaatgcaggt tacattatcc gctacatgga aaacatcgat
    11041 ccggctctgg cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg cgcgccggga
    11101 tatctgttct tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac caagccgggt
    11161 atggtaatgg gctttatcgg cctgctggtg aacatcccgg tgaactatat ctttatttat
    11221 ggtcatttcg gtatgcctga gctcggtggc gttggttgtg gcgtggctac tgcggcggtg
    11281 tattgggtca tgttccttgc catggtttct tacattaaac gcgcccgctc catgcgcgat
    11341 attcgtaacg aaaaaggcac cgctaagccc gagcctgcgg ttatgaaacg actgattcaa
    11401 ctcggtttgc cgattgcgct ggcactgttc tttgaagtga cactgtttgc cgtcgtggct
    11461 ctgttagtgt ctccgctcgg tattgttgat gtcgcaggac accagattgc cctgaacttt
    11521 agttcactga tgtttgtgct tccaatgtcg ctggcggcag cggtaactat ccgcgtaggt
    11581 tatcgtctgg gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac cgggcttatg
    11641 gtgggtgtct gtatggcaac cctgacggcc attttcacgg tttcactgcg ggagcaaatc
    11701 gccctgttgt acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt gatgttgctg
    11761 gcggcggtat atcagatttc tgactcaatc caggtgattg gcagtgggat tttgcgtggt
    11821 tataaagata cgcgttccat tttctatatc acctttacag cttactgggt gctgggcttg
    11881 ccaagcggct atattctggc actgaccgat ctggtcgttg aacctatggg gccagcaggc
    11941 ttctggatag gctttattat cggcctgaca tcggcagcca ttatgatgat gctgcgtatg
    12001 cggttcctgc aacgtatgcc gtcagccatc attctgcaac gagcgtcccg ctaataaaga
    12061 caaggcgcaa ccttcacggg ttgcgcctgt gtttttacgc aggctggagc gttgcgccaa
    12121 tcccgtcttc gtctggctgt aatttcagag cgttacacag agtttgccga ctggcatctg
    12181 ttatctcaac gcgtatatcc ccgttaactt cccctttcac attgatgaag tcaggtgaaa
    12241 tttctttcgc actggtttgc aggcaaatcg ctccctctcc gcgcaggcgt ttcactttca
    12301 gttgcacatg cgttgccgca ccagtgatca gccacagttt tccgccattc agattgatgt
    12361 taccaacaat gaaagtgacg ctgtcacctt ttaccgtact gccgtcgaga atcaacacac
    12421 cgccctgctg tacagtaatc gttgattgtt gcagctctgc gccataacca ataatcattg
    12481 aagcgccgtc cagtaatgca acactcccgg tcagttcaac cggtgcgcga tcttcctcta
    12541 cgacaaaatt ttcgtccgcg ctggtgggtg acaacaggat cactgttcca ccttgtatta
    12601 ccgcattttc cagcgtaccg gcatgcactt cagcccgccc ccccacattc accgacaggt
    12661 tttgcgtctt accggaactg tagagctgaa ggccatccgt ccccagacga taaatggatc
    12721 cattctcaac aatagtgtca gagtcttcac caccgagatt attcatggtg ccaccagcaa
    12781 gcaacgtatc actggctttc ccggccagca taaaaacgcc gccctgatta atctcacaat
    12841 tgctggcttc tccacttgga tacaccaaca attcaccacc ttcatctacc acgaccgctg
    12901 aggttgtccc attaaccaac aggccgccct cttgatcgag aatgattttt tccgcgcgat
    12961 gtccttccag tacacgcagg ttaccgccat tttccagcaa caaaccgcag gcataaccct
    13021 gatcaacgct gaattcacct tcgggatggc ggccattaac cgtagcgagc gtagagagac
    13081 taattgcgcc tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg ctgaccgata
    13141 gcgtgccatt gttaccgtac tgtgtgcatt tggcgatccc accggttgcc actttcaacc
    13201 agccagcccc cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca atcaactcgc
    13261 cttcagtaag ttgcgtcgcg ccaaggacct cgccggtctt aacaatttgc acattcccgt
    13321 cgctcatcaa gtttttcgca tcagatccca tgatttattc ctttgctgca tctgtgtgcc
    13381 tttattgcta cctaagtgta aaggctacgg aggatttatc cacgacagat ttgagatggt
    13441 ggcaaacaac tctgtttaaa ctctgataca cgaattattg ggttgtatca gatgtaaatg
    13501 cgatcctgaa taaaaatcgc ccttgcaaat caacaaaata tagccaattt gcttaaaagc
    13561 tctgcaaacg gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac ctttccactc
    13621 tgccgctaat attcgtcccc gttgtcacct acaacgttgc gttcatagct cagttggtta
    13681 gagcaccacc ttgacatggt gggggtcgtt ggttcgagtc caattgaacg caccatcctg
    13741 cgtccgtagc tcagttggtt agagcaccac cttgacatgg tgggggtcgg tggttcgagt
    13801 ccactcggac gcaccagatt ttcttaatct ggtcttttcc tttttccctc tgtttcttct
    13861 ctgtatccaa tacgttaaaa gatttacact gtcttcgtat gcgttatcag aaggagaatc
    13921 gctatggcaa ctttgttaca acttcatttt gcttttaatg gcccatttgg cgacgcaatg
    13981 gctgagcagc ttaaaccact tgctgagtcg attaatcagg aacctggttt tctgtggaag
    14041 gtatggacag aaagtgaaaa gaaccacgaa gccggtggga tctacctttt cactgatgaa
    14101 aaaagcgccc ttgcctatct ggaaaaacat actgcccgac tgaaaaatct cggcgttgag
    14161 gaagttgtcg ccaaagtttt cgatgtcaat gagccactta gtcaaatcaa tcaggcaaaa
    14221 ctcgcctgac agaatttaat caagggcggt tagcgccctt ttcatccctg tctgaaattt
    14281 ctcaaattct aaaaatctca accaaactta tctgataaca ctaaattcga aagaatgcgt
    14341 acaggtaagt aacaatgaaa aaaattgcta ttgtgggtgc cgggcctacg gggatctaca
    14401 ccttattctc gcttctacag caacaaactc cactttctat ttctatcttc gagcaggctg
    14461 acgaggccgg tgtcgggatg ccatacagtg atgaggaaaa ctcaaaaatg atgctggcaa
    14521 atattgccag tattgaaata ccgccgatta attgtacgta tctcgaatgg ctacaaaagc
    14581 aagaagccag ccatctccag cgttatggcg ttaaaaaaga aaccttgcac gatcgtcagt
    14641 ttttaccgcg aattctgctg ggcgaatatt tccgcgatca atttttacga ttagtagacc
    14701 aggcacgaca gcaaaaattt gcagtggctg tttatgaatc atgccaggtt accgatctgc
    14761 aaattacaaa tgctggcgtc atgctcgcta caaatcagga tttacccagc gagacgtttg
    14821 atttagcggt gattgccacg ggtcacgtct ggcctgatga agaagaagca accagaacgt
    14881 attttcccag cccgtggtca ggcctgatgg aagcaaaggt cgatgcgtgt aacgtgggta
    14941 ttatgggaac atccttgagc ggactggatg cggcaatggc agtggctatt cagcatggtt
    15001 cgttcattga agatgataaa caacacgtcg tttttcaccg cgataacgca agtgaaaagc
    15061 taaatattac gttgatgtcg cgcacgggta ttttacccga agccgatttc tattgcccta
    15121 ttccctacga gcccttacac atcgtcaccg atcaggcatt aaatgctgag attcaaaaag
    15181 gtgaagaggg ccttttggat cgggtattta gattgatagt agaggaaatc aagtttgctg
    15241 atccagactg gagccaacgc atagccttag agagcctgaa tgtcgattcc tttgctcaag
    15301 cctggtttgc cgagcgcaaa caacgcgacc catttgactg ggcagaaaaa aatctccagg
    15361 aagtcgaacg caataaacga gaaaaacata ctgttccctg gcgttatgtc attctgcgcc
    15421 tgcatgaagc cgtacaggaa attgttcctc atctgaatga acacgaccat aaacggttca
    15481 gtaaaggcct tgccagggtt ttcatcgata attatgcggc aatcccttca gagtctattc
    15541 gtcgactact ggccttacgt gaagcgggga tcattcatat tctcgccctc ggtgaagact
    15601 acgaaatgga aattaatgag tcgcgcaccg tcctgaaaac ggaagacaac agctactcgt
    15661 ttgacgtttt tattgatgcc cgcggacagc gtccgcttaa agtgaaagat attcctttcc
    15721 ctgggctacg cgagcaatta cagaaaacag gggatgaaat ccctgatgtt ggcgaagatt
    15781 atacgttaca gcaacccgaa gatattcgtg gacgcgtagc gttcggcgcg ttgccctggt
    15841 tgatgcacga ccagcctttc gttcagggac ttacggcatg tgcagaaatt ggtgaggcga
    15901 tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg gcgtctttcg tttgattaaa
    15961 ggtaaagcta tcagcactca acaaactgat gctgttcatt aagacgatag cggactccgg
    16021 ttcgaatatt gttttcacct tcaatggcga cggcgaagcg aactctttcg ccgtcatgat
    16081 atgccagtgc ggcgcttccc cctagcccga gaataattga gtcgaccacg cccgtgctca
    16141 ctaccgttga attttcaccg ctggcaatga tgtgtacatt gtcgccgctg ttggcaattc
    16201 tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc attactggcg acatgacacc
    16261 gttcacccaa tgtacaaacg cgtactcgca ttccggtatt cgcaatccga ctactatctc
    16321 cagcgctgct tatccgcgaa gaattaccgg ttacagcaac ccgtgcgcgt tcaccggagc
    16381 tgccaatgtg actgttaaag ccgacacttc caatccgcac gctataaccc atactggcaa
    16441 tttgcgcggc atacccagcg gttgcgattc gggcgttatc ctcacaacag ccgatgcgag
    16501 catgatcgtc gctgcgggca aactgactgc aaaatggtat ctctccgggg tctgtggcca
    16561 gtttttgcat agaggaaact tcctgatgcg cgaagttttc atccgccagc cattttttcc
    16621 acgcatactc aaccagactc tccgcccagt ccgtatatcc ttcctcaatc agtgcatgac
    16681 gaatatcggc ataactaccg ccgcgtggga aatggcgcag gaaccagcga tacatcaccg
    16741 ctccgatacg ccattctcgc aggtctgctc gtgtaatgat cattcgttag tgccggtgat
    16801 agccatcgac catgctttta aaggtttcgt gtggcgtacg ccccgtggtg caaagataaa
    16861 gatgaccgaa gataaaaaag aggcttataa atgccagagc aaaatgcgcc tgcaataacc
    16921 agtatcttac gccaggaaac acatctccca cggcttgcgg atagagacac aacagccccg
    16981 tcaatagtaa caacggcagc aatccataca tgacaccaac ataggcgacc tgctgtaagg
    17041 gattaaattt agactgtgtt gttgccggga aaggatgttc ttccccctgc ataatgccaa
    17101 acaaataaaa tcgcgtttgt tttgccgctc gttccagcca cccctgacga cgaatgcgat
    17161 agtggtgacc attatcccca acggcattga tcagcacaaa gccgagccag catgccagta
    17221 acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt aaccgcagtt gcgcccacca
    17281 tcgcaaagtg atttatcagc ccacttgcca gcaacaatac aaagagtaac gcattcgacc
    17341 agtgccataa tctgaccgct ttgctgtaaa gataaacctt ctcaccatga tcggtggctg
    17401 atttcttcac gccacgagca cgaagcaagg cgtgcagccc cagcaccaac cacatcccaa
    17461 ccagcaatac tccggcaatg atcaaccaca ccggccaaaa ttcgggagtg aactgtggca
    17521 catagttcgc taactggctc tgaaactgtt cagcatgttg cgacgggttc atacattttc
    17581 ctttttaatc aaatgttgac cgaaccggcg atacagatgc ggttttccgg caccaggtag
    17641 ctgatattga tagtatttat tatcctgcaa ccacgcctga atttctgggc tatcttcgcg
    17701 accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa atgggcggaa agccttttgc
    17761 cagtcgggac tcagcgcaga aatcacattt atctgccact ttagtgacag ggttaaggta
    17821 gcgaacctgg tacggacaag cgccaatgca ataactgcag ccgatgcact gcgatttttc
    17881 tacccgcacg atcccctgct catcacgcca cgatgccccc gtcgggcata cgtcgatgca
    17941 cggagcatct tcgcagtgct gacatgactg gcgaaaaaag tgatactgcg tttcgttgtc
    18001 gttatcggtg actggaatgt gtgctatcga taaacggctt ccctgagcgg ggacgtggtt
    18061 agttttacgg catgcgcggg cgcaaatatt acagccattg caccgtgact cgtcatgaat
    18121 catggcatac cgaacttcct tttcttgcct cgtgttcgct aacagagaag acgcagaacc
    18181 agtaaaaaag attactgttc ccatccccag aacaaatttg cgtcgagtaa aggacatctg
    18241 attatcccct aatgcagtgg ctgcaactgt ccaccctatc catgacatct ccctgaaatt
    18301 tcaggggttc tgaaactgct ctacctcgaa ccattcgcat aagccataag ccttgccata
    18361 ggtcataaat cgctcgctaa acaagtacaa caccgccgtc gatgactcca cctgccgtat
    18421 atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg gcttcaatct gcgcttcctg
    18481 gaagtaatat ggcgcttgca tcagcatggc gactttgtaa ggacgaggat acgtctggca
    18541 ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc tgctccacaa cttgcaaaga
    18601 catcgccgcg tagttttcac tcatggcctg cgttgagtaa tagtaatcat cctgacttcc
    18661 cgtcatacaa gcgatatccg cgtagtctcc gttctgctta atgctttcca gcaaaactga
    18721 cagttcctct tctgcaaccg aatagggtgg cgagagaaac acggtgcgcg ccaccagctg
    18781 cccagccgca gaatgctggc gaatgaactc agccaggatt tcagcaggtt ctggttcggt
    18841 taccgtatca acggggtgac gcatcccctg caaccattgc cgcttcgcct cgtcaacttc
    18901 gctgactttc gctaacggca attcatcctg atggttcatc ttacccattc cttacgcagg
    18961 cagtagattg tgtgctgcca gatcggcggc aatatcttcc agccccagac gctgcaatgt
    19021 ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt tctttgtaga acatcgtgag
    19081 cgaagcatgc atatcgtcac gatccatttt gtcagtacct tcagtaaaga ccgggatctg
    19141 cggatccttg tcgaaaaccc aggaacagat aagatcgtgt tcattacgca tatctttggt
    19201 ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag atacgctctg cagctaaatc
    19261 taatttttcc tgagtcatct cttcgccggt gatcgctttg aagaatttgg cttaaagcgc
    19321 cagatcgccc cggtaattac ggcttttcag cggcgaaacg gtcattggcc agacccagtt
    19381 gcacagagtg acggcgttat gcaaacagac ccgcaacagc gaccactttg catatttaat
    19441 ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg taagcatctt cagaaccaaa
    19501 aagttctttc gccacttcac gttgcagttt caatggcaag ccggaaccaa taaagttgat
    19561 atgggtatgc gtcatgcagt cacggttgaa catacagtta acaatggaac cgacctgcgc
    19621 tgacgcttca ttggcatggt gaaccggata gccaaacggc gaccagagtt tatttttcgc
    19681 gtagccccag tactctttac ccaaattcca gcgttctgcg atggcatatg aaccatcagc
    19741 caggtgactc agctcaccca cacgatgcgc cagacggtag taaaaatctt taatgaagtt
    19801 aacgtcaccc gcttccagtt gatcccagcg aatttctgca tactcttcag ctggcagaac
    19861 acgcttgaac acacctttgc tgtaacaata agtaaagtcg cgatgcaact gcccgtagtt
    19921 acaccatagg ccgtagtcat cgaacaaatt cagaccaacc aggttaccaa tcacacgacc
    19981 atcgtcttta tcttcaaaat ctttcggccc gttcgggaag atggtggtat ggacaaagtt
    20041 tgctacacaa gtgttaccac ctgtgctggg cacgccaaac tctttcaccc gaggaatatt
    20101 catttgggtc atacaacgga tcgggcaaga gtggcagccg ctcattttta ctgtgtattt
    20161 ctctgccgcc ggtcctaagt caaaaacgga tttataggta cgaaagccga ccgtattctg
    20221 attgcctggc ggaatttcac ccgtttcaat cggaccacct tcagccgcgc cccaaaacag
    20281 ccctttacgt gctgtccagc gtgacttggg atctgaatac tctgcccacg attgtggcgt
    20341 acttggcacg acatggttgt tattcgcacc aataagttca gtcatcatgt aatcattgag
    20401 acgcttcatc tcctgacgat cggcaatgtt gacccctttc gtcccttcaa ccgcaatcgc
    20461 tttcaggttt ttcgaaccca ttattgcgcc agttcccgca ccgccgctgt ggttacggct
    20521 attcaacatg ccagagagag gaataaggtt ttccccagcc tgaccaatag ccgccacaca
    20581 ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt gcgcgcgtcc cttttcccca
    20641 taagaaatcg gctttttcca ggctaacttt gtcatcttta atcttcagcc ataccggtga
    20701 tttcgccttc ccttcgataa taatgacgtc gtatccagcg aatttcattt gcgctgcaaa
    20761 aaagccaccc atatgggcat cgcgactaaa tttcctttgg taaaagtaga aagtgaggtg
    20821 atatttacgc gagaactaca gggggcacca gatccagtta atgggccggt agcaaagact
    20881 aatttattcg cttcatcgaa aggtttcgtg cctagcggta cttcgtcata cataattttg
    20941 tagccgaagc ccatgccacc gacaaaactt ttaaacttac tggaatcttc gagggtaata
    21001 tttcctgtcg tgagattgac tcttaatata ttacctgtcc aaccgttagc catgattttt
    21061 tcctttgcaa gattcacaca gtaatatctt tccactcgat aatttttaac gcccctgttg
    21121 ggcaggcgtt tgcgcattca ccgcataaca cacatttcga ggatttttta ctttcggtat
    21181 ttacggtggc catcatccac gggcatgccg tagtacaggc gctacagcca atacaacgtt
    21241 tatgatcgac ggtaatacag ccttctttct gctgccaggt aatcgcacca atcgggcaga
    21301 cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc cgcggtatag ttgagatcgc
    21361 catacaggcc gccgccagag ccaaccccgt tgtcgccaaa gaaataattg cgatggattt
    21421 tgatacggga gaagaatgtc cctactgagc catcgttgaa gttggtacaa gagatttcac
    21481 agcggtggca acccgtgcat cgcgctcgct gggtcaccag cacccctttg ggtgtattta
    21541 tcaaccctac tgtgccgcta tcaatatctt cctgtttgca acccaaaagt gacagtaacg
    21601 caggggcaat tgttagacca gcaaggcctt ttcctgatat acgcaagaat tctaatcgcg
    21661 ttaagccaat atctaatagt ggacgatcaa ccgggttcat ttatttcgca tcctcttcgc
    21721 aaacacagtg ctgacaattc agcagcctga tattaagcat tcaactaata cgctgctgac
    21781 tctgtcccca acaccagata ttttccctaa cccgatagtg tatgagaaaa gatttaacgc
    21841 cgataaacta atcctttatg gttaggagaa aaaataatgt gattattctg agcccttaac
    21901 attgatcgtt atcaattaaa attacaaagc aagaaattat aagcgaactg atatttatta
    21961 tcatttgaaa taaatttaac ttaagcgaca atcatttgtg acgttcattg cagaaaataa
    22021 aaataacatt atcgctataa attaaaatac ataccgaaat tcgtataatt tcagcagttg
    22081 ttatttagaa tgattaactg gaccgcaact gaagcaagaa tatcaggatg aagcttaccg
    22141 cctcatcctg aatggtcatt ttaccagtgc gttaatgacg gtttccatcg cctgcttttc
    22201 cagctcactg cgctgaatgc gtttattggc taatgctgta cgtaat
//
LOCUS       DAJTUN010000059        21972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTUN010000059 DAJTUN010000000
VERSION     DAJTUN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.059"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..355)
                     /gene="pgrR"
                     /locus_tag="OO470_002149"
     CDS             complement(<1..355)
                     /gene="pgrR"
                     /locus_tag="OO470_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6251391.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLK"
     gene            493..1413
                     /locus_tag="OO470_002150"
     CDS             493..1413
                     /locus_tag="OO470_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6251392.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1413..2348
                     /locus_tag="OO470_002151"
                     /pseudo
     CDS             1413..2348
                     /locus_tag="OO470_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2366..3004
                     /locus_tag="OO470_002152"
     CDS             2366..3004
                     /locus_tag="OO470_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6251393.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3295..4023
                     /gene="mpaA"
                     /locus_tag="OO470_002153"
     CDS             3295..4023
                     /gene="mpaA"
                     /locus_tag="OO470_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6251394.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(3998..4957)
                     /gene="ycjG"
                     /locus_tag="OO470_002154"
     CDS             complement(3998..4957)
                     /gene="ycjG"
                     /locus_tag="OO470_002154"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6251395.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5076..5582
                     /gene="tpx"
                     /locus_tag="OO470_002155"
     CDS             5076..5582
                     /gene="tpx"
                     /locus_tag="OO470_002155"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6251396.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5626..7167)
                     /gene="tyrR"
                     /locus_tag="OO470_002156"
     CDS             complement(5626..7167)
                     /gene="tyrR"
                     /locus_tag="OO470_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6251397.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7315..8376)
                     /locus_tag="OO470_002157"
     CDS             complement(7315..8376)
                     /locus_tag="OO470_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6251398.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8373..9770)
                     /locus_tag="OO470_002158"
     CDS             complement(8373..9770)
                     /locus_tag="OO470_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6251399.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9926..10924
                     /locus_tag="OO470_002159"
     CDS             9926..10924
                     /locus_tag="OO470_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6251400.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11035..11907)
                     /gene="ompG"
                     /locus_tag="OO470_002160"
                     /pseudo
     CDS             complement(11035..11907)
                     /gene="ompG"
                     /locus_tag="OO470_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(11980..13061)
                     /locus_tag="OO470_002161"
                     /pseudo
     CDS             complement(11980..13061)
                     /locus_tag="OO470_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13075..13734)
                     /gene="pgmB"
                     /locus_tag="OO470_002162"
     CDS             complement(13075..13734)
                     /gene="pgmB"
                     /locus_tag="OO470_002162"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6251401.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13731..15998)
                     /gene="ycjT"
                     /locus_tag="OO470_002163"
     CDS             complement(13731..15998)
                     /gene="ycjT"
                     /locus_tag="OO470_002163"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6251402.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(15995..16618)
                     /locus_tag="OO470_002164"
     CDS             complement(15995..16618)
                     /locus_tag="OO470_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6251403.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16615..17037)
                     /locus_tag="OO470_002165"
     CDS             complement(16615..17037)
                     /locus_tag="OO470_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6251404.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(17059..17834)
                     /locus_tag="OO470_002166"
                     /pseudo
     CDS             complement(17059..17834)
                     /locus_tag="OO470_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17852..18904)
                     /locus_tag="OO470_002167"
     CDS             complement(17852..18904)
                     /locus_tag="OO470_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6251405.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(18935..19777)
                     /locus_tag="OO470_002168"
     CDS             complement(18935..19777)
                     /locus_tag="OO470_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6251406.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVMAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19764..20645)
                     /locus_tag="OO470_002169"
     CDS             complement(19764..20645)
                     /locus_tag="OO470_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6251407.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20666..21949)
                     /locus_tag="OO470_002170"
                     /pseudo
     CDS             complement(20666..21949)
                     /locus_tag="OO470_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
BASE COUNT         5384 a         5936 c         5389 g         5263 t
ORIGIN      
        1 atttcaggaa tgtgcgcatg gctggtaaca atatcgtttt tgctgcatgt tctacagtag
       61 taatacgtat tgtcccggat gggcggttct gcagatcgct cagggatgtc agggcagaat
      121 ctatgtcatg caatataggg ccaagaacag acaaaagatg ctcgcccgct tcagtgggta
      181 caacgctgcg cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct atacggcgca
      241 ctatctggct taaagctgac tgcaccatgc tcaggcgggc tgctgcgcga gtgaagctac
      301 gctcctctgc aacgacgaca aacgccatca gatcagcgat ttcttcacgc ttcattcagt
      361 atctccatac aatctattaa tcatttaaat gataggtcta atcatgattt agggtctaat
      421 caacttaatg atcaatatct atagtgatat caccaaccgt caaaacgaac cgacaactcc
      481 atgaggaact gaatgatgaa taataaagtc agcttcacta acagcaataa tccaaccatc
      541 tctttgtctg cagtgatcta tttccctcca aaatttgatg aaacccgtca gtatccggca
      601 attgtggtat ctcacccagg tgggggcgtt aaagagcaaa cggccggaac ctatgccgaa
      661 aagctggcgg aaaagggatt tgtcactatt gcttatgacg catcttatca aggtgaaagt
      721 ggcggcgagc cgcgtcagtt agaaaaccca tacattcgta ctgaagacgt cagtgccgtg
      781 attgattacc tgacaacgct ttcttatgtc gacaataccc gaattggtgc gatgggaatt
      841 tgtgccggtg caggatatac agccaatgct gcaattcagg atcgccgcat caaggccatc
      901 ggtaccgtta gtgcggtcaa tattggttcg atgttccgca atggttggga aaacaatgtg
      961 aaatctattg atgctctgcc acacattgaa gctggctcaa acgcccgaac cagcgatatt
     1021 agcagcgaag aatacgccat tatgccactg gctccaatga aggaggccga tgcgccgaac
     1081 gaagaactgc gtcaggcctg ggagtattac cacacacctc gtgcgcagta tccaacagca
     1141 ccgggttacg ctactttgcg cagccttaac cagattatta cctatgatgc ttaccatatg
     1201 gcggaagtgt acctgactca gccgatgcag attgtggcgg gcagccaggc agggagcaaa
     1261 tggatgagtg acgacctgta cgatcgggca tcaagccagg ataaacgcta tcacattgtt
     1321 gaaggcgcaa accatatgga tctttatgac ggcaaagcct atgtggctga agcaatttca
     1381 gtattagctc cgttctttga ggaaactctg taatgaaaca tattcaaatc cgcaatagtg
     1441 atatggcctg gcatattgct gccaatattc agtttccacc aaattttgat gagtcaaaac
     1501 agtatccggc gattatcagc gtccatcctt ttggcagttg taaggagcag acgtctggca
     1561 atatctatgg caaggcattg gctgaaaaag gctatgtcgt tctcgcttat gacgccagtt
     1621 tccagggaga atctggtggg tagcctcgtt ggatagaaga tccgacacag cgcgtagagg
     1681 atattagccg tgttatcgat tatgcagtaa ctctgccata tgttgatgct gaacgcattg
     1741 gcgtgctggg tgtgtggggg ggggggggtg gctatgccat taatgccgcg ctgacagaaa
     1801 aacgaattaa agctgtagtg agtattacgg gtgttaatat cgggcgtctt tttcgggaag
     1861 gtttcagcaa ttatgatccg attggcgtat tgaatgcaat ggcgtcacaa cgagcaaaag
     1921 aggctcgtgg tggagaatta cagatcaacg aattacttcc cgccagcctg gatgcagcaa
     1981 aagcccatgg actgaccgaa cgcgatgttt atgaagcaac tgactactat aaaacaccac
     2041 gcgggcaaca accgggaggt gcgacaaaaa tgttgttctc acatgcacag aaaacgctgg
     2101 cctgggatgc cttcgcattt actgaagtgt tactgaccca gccggtgatg gtagtagttg
     2161 gtgaaaaagt cggtgccttc ggtgcttatc gtgatggact ggaagtctat ggccgggcga
     2221 tggtttcgca ggatcgtcag ttagtttcgt tacccgattt ctcacattat gaactatatg
     2281 acaaaccgga agcagttcag gaggcgctgg caaaagttat tccttttttc aacacccatc
     2341 tgggataagt ttcatgagaa atattatgaa aaatgtactg attcttggtg ctggcggtca
     2401 gatagcccgc catgtgatta atcaactggc agataaacaa actattaaac aaactttatt
     2461 tgcccgtcag ccagcaaaaa tacacaaacc ctatccaact aatagccaga ttattatggg
     2521 ggatgtactg aatcatgcag cactgaagca ggccatgcag ggacaggata ttgtgtatgc
     2581 caaccttacg ggggaagatt tagatattca ggcaaattca gtgatcgctg cgatgaaagc
     2641 ctgtgatgtt aaacgtctga tctttgttct ttccctcggt atttatgatg aagttccagg
     2701 aaaatttgtc gaatggaata atgcagttat tggtgaaccc ctaaaaccat tccgcagggc
     2761 tgcggatgca attgaggctt ctggactgga atatacaatt cttcgccctg cctggctgac
     2821 tgatgaagat attattgatt atgagcttac ctctcgcaat gaaccattca aaggtacgat
     2881 cgtatcacgt aagagtgtcg cagcattaat tacagatatc atcgataaac ctgagaaaca
     2941 cattggtgag aatataggca tcaatcaacc cgggactgat ggcgataagc cattcttcat
     3001 gtaaatcaat tatatagata ccatcgtact gtactatgtg cagtacgatt tattaaactt
     3061 attaaggtga actgatcaat cagatgcgat taacatgtgt agaaccagta agaataaaat
     3121 acgccatgta tataacatta cgacaattct aatatctgca catcaatgaa aatgcaaaca
     3181 acttccggca aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata
     3241 atccgcaaaa tttgcacaac actgcctgta atacttcctg aaccagagac gatcatgacc
     3301 gtaacccgct cacgcgccga acgcggcgca tttccgcccg gaacagaaca ttacggacgt
     3361 tcattattgg gcgcgccgtt aatctggttt cctgcccctg cagccagccg cgaaagtggt
     3421 ttgattctgg ctggcactca cggtgatgaa aactcttcgg tcgtcacgct ctcctgtgct
     3481 ctgcggactt tgacaccttc tttgcgccgc catcatgtgg tgctgtgtgt gaatcctgac
     3541 ggctgccagc tggggttacg ggccaatgct aatggtgtgg atttaaacag aaactttccg
     3601 gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga acagcgccgc tgaagaacgt
     3661 gatgtcgtat tactgacagg tgacaaacct ggctccgaac ctgaaaccca ggcgctgtgc
     3721 cagcttatac accgcattca acctgcctgg gtggtctcat tccacgatcc actggcttgt
     3781 attgaagatc ccagacacag cgaattaggc gaatggctgg cccagtcgtt tgaattgcct
     3841 ctggtaacca gcgtcggtta tgaaacgcct ggctctttcg gcagttggtg tgcagatctc
     3901 aacttgcatt gcatcaccgc agagtttccg cccatctcct ccgacgaagc cagcgaaaaa
     3961 tatctctttg cgatggcaaa cttgctgcgc tggcatccta aagatgcaat tcgcccgtcg
     4021 tgaactgaag cgccggttcc acatctaccg ccagccaggt cggtccgtca agatcggcga
     4081 aactgacctg cggcaccagc ggtaaagcgg cgctaatggc acgagaggta cacaacatgc
     4141 agcccagcat cagactgaaa ccttgtgcac gcgcttcagt cgccagcgct tccgtcagac
     4201 caccggtttt atcgagctta atgttaacca tctcatagcg ccctttcagc gccttcagat
     4261 tgctacgagt atgacaactt tcatcagcac aaatcggcaa cggatgaata aaattctcca
     4321 gcgccgcatc gtcctgcgca ggaagcggtt gttcaagcat cgcaacgcct aaatccgcca
     4381 atagctggca acgcgccgcc aacccttctg cacgccagga ttcatttgca tcaacgatca
     4441 gcgtggcatc gggcacagcg gtgcgaattg ccaccatccg ctcactgata agatggttat
     4501 ccagcttcac tttcagtaat ttcgcgcctg cctgccagag tgttgatgca ctattggcca
     4561 tctgatcagg cgtaccgatg acaaccgtct gtgcagtgat aactgtctcg ggaagcgtta
     4621 tgccgatcaa atcagccagc gattgctgct gttttcgcgc cgcgagatcc cacaatgcac
     4681 aatccagcgc attacgtgct gcgccggcag ggagaatttt ttgcaactcc tcccgtgtca
     4741 gccctttctc taattgcggc acgacgctca taatttgcgc cattaccgag gcatcacttt
     4801 ctccataacg cggatacggc gtgcattcgc cggtgccttt aataccctct tcttccagtt
     4861 caaccaccac cacgcgcgct tcactgcgac ttccccgggc aatcacaaac ggggtatgta
     4921 agggccaggc ttcctcaaat accttaacga ttctcatctt tactccttgt tgtcgcggtg
     4981 gtctgcggat taagcgcaaa tagcgtttgc tgtgttattg acagttagca taaactaggt
     5041 gtgacgttaa ctatatgtaa acaggaaaga taatcatgtc acaaaccgtt catttccagg
     5101 gcaacccggt tacagtcgcc aattccatcc cgcaggcggg tagcaaagcg cagactttta
     5161 ctctcgtggc aaaagatctg tctgacgtca ccctcggtca gtttgcgggt aaacgcaaag
     5221 tgctgaacat tttcccgagt attgataccg gtgtttgcgc cgcatcagta cgtaagttta
     5281 accaactggc aactgagatc gacaacaccg ttgtgctgtg tatctctgcc gatctgccgt
     5341 tcgcccagtc tcgtttctgc ggcgcagaag gtctgaacaa cgttatcacc ctctccactt
     5401 tccgtaacgc tgaatttctg caagcctacg gtgtggcaat tgctgatggc ccactgaaag
     5461 gtctggcagc gcgtgccgtt gtggttattg acgaaaatga caatgtgatt ttcagccagc
     5521 tggtggatga aatcaccacc gagccggatt acgaagcagc tctggctgta ctgaaagcat
     5581 aagttaatat gcctgatggt gttgcaccat caggcatatt cgcgcttact cttcgttctt
     5641 cttctgactc agaccatatt cccgcaactt attggcaatc gcggtatgtg aaacgccgag
     5701 acgttttgcc agtttgcgcg tgctgggata attgcgataa agctgggtta ataccgagcg
     5761 ttcaaaacgg ctggtgattt cgtccagcga accttccatc gcatcttcgc ccacggctac
     5821 cgttgcggcg tcataatccg gcaacaaaat atcctgtgga cgcagctcat aaccgtccag
     5881 ttgtgtcagt gcgcgataga tagcgttctt taactgccgc acatttcccg gccacgcata
     5941 acgtgtaagt acagtattca ggtcagcggc cagtttcgga cgcggcacgc cctgctcgtc
     6001 ggcaaagcgg gcgacgaaca gctcagttaa cggcataatg tcctgcggac agtcacgtag
     6061 cggcggcaga ttgagcgtca acacgttcag acgataatag agatcttcac ggaacacgcc
     6121 tttttgcacc agttcgacca gattcttctg cgtagcgcaa atcacccgca catcgacatg
     6181 cacctcatgg tcttcgccaa cccgacggaa agtgccatca ttaagaaaac gcagtaattt
     6241 cgcctgcatc cgtggtgaca tttcccctat ttcatccaac agcaccgaac caccgttcgc
     6301 ctgctcaaag aaccctttct tcccttccgg agcatgacca aacagttcac tctcgaccgc
     6361 atcttctggt atagacgcgc aattcagcgc caggtaaggt ttgcccgctc tggggcttgc
     6421 ctgatggcag gcgtaggcaa aaagatcttt acctgtacct gtgtcacccg taatcagcag
     6481 cggcgcgctt agcatcgcca gtttctgcgc ctgttcgaca acgtgcttca ttttcgggct
     6541 gacggcgaca atttggctga aggcgctgac gtcctgggcg gcgacatttt gcaactggcg
     6601 gcccatacga atcgttgatc gcaacatcac caccgcaccg gtcaggacgt gttgatcatt
     6661 ttcatcctga agataaacag gcgtaatctc catcaggaaa ttctgcccat taataacgac
     6721 atgctcgtta tgcgaatctt gcggttcgct ttccagccaa cgtaaaaaat taaagccgtt
     6781 aatcaattgt gcggcggtat ggttgcgcag gcgatccaat ttttgcccaa aaagctgaca
     6841 gctcgccggg ttcgccatat ccactttgct tttcatatcg acagagagca caggttcagg
     6901 caacgcctcc agtaacgcgc tcaacgccag atgctcacgt tcggaaggca tccacgggac
     6961 agtacgcaca tcggtaacac ctgcaatacg gcgtatttcg gccatcagac tgctgaaact
     7021 ctcaaactcc agttcagcaa aattgaggta gattcgccca atgggatcaa tctcaatacc
     7081 gcgtaaatca atgcctctta gcacgagtag atcgagtaat tcgcgggtca gaccgagtcg
     7141 gtcttcacaa aagacttcca gacgcatggg aaccttcacc tgaaaaaaga acaattaata
     7201 tgatgatatg acactatttg gatagcagga aggtttctgt caacaatcat tgacacaaag
     7261 acggaaaaac gggcacttcg gcgtaaagat atccggtcag cacccgatat tgcattattt
     7321 ttcgctgggc gttttgcctt tttgcagagt ttctttcacc tgaccgataa gctgacgacg
     7381 gaaatcccca aggcgaggtt tgtcatcgtc aatccacggc agcgggcggc aaagctccat
     7441 agctttaatc ccgagtcgta ccgtcagaag tccagcacca attccctgag ctgcgcgggt
     7501 agacaaacga gcagcgagat cttgcgacat ccagtccatc cccacttcgc gcaccagttc
     7561 gctggctccg gcaaaagcga tattcagcaa taccagctta aacagacgca aacggctgta
     7621 ataccccagt tcaatgccat acagcgtggc aatgcgatta attaaacgca gattgcgcca
     7681 ggcgataaac gccatatcga ccaacgccag cgggctgacc gcaatcatca acgttgattc
     7741 cgccgccgaa tggctgattt cgcgccgcgc ctgggcatct aaaactggct ggaccaaatg
     7801 cgcatacagg ctgaccactt cacgatcgtt ctgcgtttca tggattgagg cataccagcg
     7861 ttgcagcgcc ggatgcgact gatcaatacc cgcctgctgc gccagttttt cgcaaaatgc
     7921 gcgacctttg cccgtgccgt ggctatgcaa taaatcacgc gcttcgtcgc gttcatgggc
     7981 gcgctgtcgc aagcgccata agcgccgcca ctctgttacc acagaaccta cgccagcgcc
     8041 gataatcaat gccccagcgg cacatccacc cagcgctacc cagtcctggg tttgccaggc
     8101 attcattgtc cactgtatac cctgcccgac aacgcttgcg ccaaacagag ccagcccgcc
     8161 catcaccatt ttgcgccaca ggctgcgttt cggacgtaac gctgcgtcca ttaccgcttc
     8221 gacttgcccc tcttcttcct gcgcttcgtc gagcgtggcc ggggcaaaat tttgcgcctg
     8281 attttcgtca aaggtctgct gcgccctgaa ttttggattc tgttcgacct ccagcggacc
     8341 gtcgaaatca atacgtggtt ttaacggttc ggtcatcgca atttatctcc tattaaaaat
     8401 tccagcgcgg catcaagacg aatatgcggc agcggtttgt cgacatccat cacctgcggg
     8461 cgaaacgctt caaactggaa cccttgcttg tcccagaacg cctgaccggg caaacgcgca
     8521 ggaacttcac cggggtaaac ggtgagaggc gcaccatcgc tgagccggtt accgcgcaat
     8581 gccggaattt tctcgccgtt gacgtcaata atgccgctgg tggtcgcctg caccgaggcc
     8641 agccccaggc aatccatact aatcctttca aacgccgcat tttgccaggc atcctgaatc
     8701 agttgttgca gtaatgaaac catattggcg tgctgatcga ccgttacatg gtccgctttc
     8761 atcgcggcaa acagcagttt gtcgataacc ggcgagaata agcggcgaaa cagcgtgcgc
     8821 tgcccgtagt ggaaactttg catcagctgc gtcagtgcca gacgcatatc attaaatgcc
     8881 tgtggcccac tgttgagagg ttgcaggcaa tccaccagca caatctggcg gtcaaagcgc
     8941 agaaaatgat tcttatagaa ccccttcacc actttctcgc agtaataatt aaaccgctcg
     9001 cgcagcattc cggcattggt gtgcttatcg gcctgcgcca gtttggactc gtcccaggca
     9061 tcgacatccg gccacgggaa gaattgcagc gcgggcgcac ctgccatatc tcccggcaag
     9121 acaaagcgcc caggctgaat aaagtgcagc ccctgctgtt tacagtggtg gagataatcg
     9181 gtccacacgg cggcaatgtc tgccagccgg ttttcgtcgg caggcgctag cgggtcaagt
     9241 ccttcgcaca tcattcgcca tttcgccgac cattcgccgc gctgaccatt gagtaagccc
     9301 gtcatctggc gcgaccagct taagtaatcc tgcgccagca tcggcaggtc gagcaaccat
     9361 tcgccgggat aatccacaat ttccagatac agcgtggagg tatccttaaa gtggcgcagc
     9421 agcgaatcgt tcgatttaaa acgtaacgct aggcgaattt cactgacgcc gcgcgtaggc
     9481 gtcggccagg cgggaggatc accatataac tgcgccagtc cttcatcata ggtgaagcgc
     9541 ggaatgccaa agtcacgctg gggaatgcgt tttacgccca gcaagcgctc ttcacgcacc
     9601 gcgcttaaca gcggcaaacg tgctccggca tgaatattga gcaactgatt aaccatcgcg
     9661 gtgataaacg ctgttttgcc gctgcggcta agtccggtta cagcgaggcg cagatgtctg
     9721 tcgacacccc gattcaccag cgcattgagt tcatttttaa gtcgcttcat tgctgtcctg
     9781 tgctgctctg aagaccagaa aaaaaatcat tcgtaaggat atgaatgtgg gggccaggcc
     9841 attgattatc aactgcccgt gttgtcatcg acgggcgaac tccataagat gtgcagcatt
     9901 cacgttcgga cggagttcct gacctatgtc ccctactatt tatgatattg ccagggttgc
     9961 aggcgtatca aaatccaccg tatcacgcgt gctgaataag caaactaata tctccccgga
    10021 agcgcgcgaa aaagtgttac gggccattga agaattacag tatcagccaa acaagctggc
    10081 ccgcgcgctg acctcttcgg gttttgatgc cattatggtg atttctaccc gttcgaccaa
    10141 aactacggcg ggtaatccgt ttttctctga agttttgcat gccatcaccg ccaaagcgga
    10201 agaagaaggt ttcgacgtga tattacaaac gtcgcacaat ccggcagaag atctgcaaaa
    10261 atgcgaaagc aaaattaaac agaaaatgat taaaggcatt attatgctca gttcgccagc
    10321 ggataagtca ttttttgccc aactcgataa atacgatatt ccggtggtgg taattggcaa
    10381 agttgaaggt caatatgccc atgtttattc tgtcgatacc gataattttg gtgacagcat
    10441 tgcgttgacc gatgcattaa ttgaaagtgg gcatcaaaat attgcctgcc tgcatgctcc
    10501 gcttgatgtc catgtttcgg ttgatcgcgt taatggctat aagcaaagtc tgtccgcgca
    10561 taatattgca gtgcgtgata aatggattgt tgacggcggt tatacccatg aaacagcact
    10621 gcaagcagca cggcaattat taagtcagtc accgttgcct gaggcagtgt ttgccactga
    10681 cagcctgaaa ttaatgagca tttatcgtgc ggcggcagag aaaaatattg ctattccgca
    10741 gcagttagcg gtggtgggtt atagtaatga aacgctgtcg tttattttaa cgcctgcacc
    10801 aggcggcatc gatgttccga cgcaggagtt aggtcaacaa agctgcgagt tattattccg
    10861 cttaatttcg ggaaaaccgt caccacagaa tattaccgtt gccacgcata tgacgctgaa
    10921 ataaatacca gctaccttgc ctgaatgccg gatgcgatgc tggcgcatct tatccggcct
    10981 acatttatag atcggataaa gtactcgcgc cgcatccggc aatttagccc attatcagaa
    11041 cgagtaattt acgccgacac ctgcataatg gaatttatcg ctgtcgcctt cgtcgtgatc
    11101 ctgccactca aacgcgtatt ccagcgaaac ggaaagtccg ttctggaaat cataaccgta
    11161 aaataaaccc acacggttaa aatcatggcc ttcacgttca atatcatcct gccagtccca
    11221 gttactctag cgatccagcc caatgcgcgt atacggcgtc accgagtggt tgccgagcgt
    11281 cagcggcaaa taggcgcgaa tttcttgtgt ggaaaactca ccgttattgc ggctgtcgtc
    11341 catattgaag ccgcgctcga gataatagtt cactcgcaaa gcaaccgttt cgttgaaggt
    11401 atattgcaga cctgtttccg tttcgacacg ggtatcagcg taaccggtcg tgttcaaatc
    11461 gttggcaaat ttatacatcg acaaccaacc gttgaaacgt aaatcatcag tcagtttcac
    11521 atcccagtct ggcgcgattt tccagcgctg catattcgcc gtgtctttgc ccggttcatc
    11581 aacgtagtga taaccataat tacggaaacc gccggtcagg ccgaaactga aatcatcgct
    11641 ttcgaggaac tgataatgca cctccagctc cgggcgatca aacctcgttc cacgtttacc
    11701 cgcgctataa tctaccggcc cttcctgata ataggccaga gcaattctcc acggcccgtt
    11761 ggcggcatta aaatagactg aaggctccgc cagcccatcc atatcttcgc cataaccctc
    11821 gacgttttct atttcgtaca tcgcaccgat attaaagtgc cagtcgctct tttcctcggc
    11881 ctgtgcgcag gccattcccg cacacatcac cagtgcggta cagggtaata actttttttg
    11941 ttccttgtat tatcctgggg gaatgccttc cccctgtatt tagcgaattg ctatttccgt
    12001 ttctgcatca aagaaatgac atttcgtcat atcaaaatga atagtgatat tttctcctgc
    12061 atgataatca tttaacgcac cagcacggac cactaactcg tgcccccaac cgtggtgtag
    12121 agcataaatt ccgcaccggt taattctgcc acgctaattt tggcggaaat gttattttcc
    12181 tcttgcgcgt ccggatgaat atcttccggg cgtattccca tcacgatggg cttatgcaaa
    12241 ctttcctgtg ttttcagaac cgctaatttc tcttcgagaa tggttaattt aagcgtttcc
    12301 gtaacgaatt tatcgccatc gatcgtgccg cgaataaaat tcatcgctgg tgatccaata
    12361 aatccggcaa caaacatatt cgcgggttgg ttataaacgg ttttcggcgc acctacttgc
    12421 tgaacaatcc cgtctttcat aatcacaatc cgcgccgcca tggtcatcgc ttcggtctga
    12481 tcgtgggtca cgtagatcat ggtggtgttc agtttctgat gcagcttgct gatctctgca
    12541 cgcatttgta cgcgcagctt ggcatcaagg ttagagagcg gttcatccat taaaaacacg
    12601 cccgcttcgc gcacaatcgc ccgcccgagc gccacacgct ggcgttgccc gccggaaagc
    12661 gcgcccggct tgcgtttcag gtactcacgc aggccgagaa tttgcgccgc ccagttcacc
    12721 cgctcatcaa tcacctcttt ggcgattttt tgcatcttca ggccaaacgc catgttgtcg
    12781 taaaccgtca tatgcggata caacgcgtag ttctggaaca ccatcgcgat attgcgtgct
    12841 ttggctggaa cgtcattcat tcgtttgccg tcgatcaaca gatcgccgcc gctgatctcc
    12901 tcaagcccgg caatcatgcg cagggtggtc gatttaccgc agcccgacgg gccgacaaat
    12961 acgatgaact ctttatcggc aatttccagg ttgaagtcct tcaccacatg cacctggtta
    13021 tcgtagattt tttgaatatg ttgtaacgaa agctgagcca tgttgattcc tttgctatac
    13081 gttttgccag aaggccgata accgcgacca ggtcagggag tccgttgagg gcaacagtaa
    13141 ttgcgccccg gttaagcccg caccgatccc caccgagcgc ataccgctgg cgttaatggc
    13201 gtcaatgccc gcctgcgcat cttcaatgcc gatacatgcc tgcggcggca cgcccagccc
    13261 ggcacaggca gcgagaaaga tttccggatc cggtttcgag tttttaagtt gggaagcatc
    13321 cgcgcagaag gtgaaaaact cgcgcagctc cagcgccgct aaaatcgtcg gcgcattcag
    13381 ggagacggaa gccagcccta ccggaatctg ctgtgcacgg agctctgcca gcaaattgcg
    13441 aataccgggc agcacagcat taaccgtcag ctcgcgcagt gagtggacgt aaagcagatt
    13501 tttgcgatac gccagttgcg cccgctcctg cgggttaaag tcgccctctt tgcccccgtg
    13561 ttgcagaatg cgccgcagag actcatcgcg gctgatccct tttagagatt cgttaaactg
    13621 cgcatcaata ctgatgccaa tttcagcggc aatctgctgc cacgcctgga aatgcagatg
    13681 cgcggtatcg gtgattacac catccagatc gaaaattacc ccttgcagtt tcattcatcc
    13741 tcctgatgcg tggtagcggt cccattgaag ggcaaaataa aatcacccaa acagaaaaca
    13801 gattcttcag ccacggatat aagctgaccg ttcaaacgca gtgaaacggg cgcagaagtt
    13861 cgaatcgcaa tacgctgcgc gtcgagagtg acctgtaatt cgcagccctg ccagaacaga
    13921 gggaaagaca actgttgcca ctgctcaagt aacgccggat ttagatgcaa ttcaccgtca
    13981 cgcacgctca ccccggcaaa accctgaatc gcccccagcc agatagcgcc agttgcggca
    14041 gcatggatac catcatcaca actatgcgga tcagcaccaa gatcgatttc agtcccctcg
    14101 cgccagaact gataactttg ggtcagcagg ccacagcgtg cagcaacaat gccgtggatt
    14161 gctttactta atgacgagtc gtgaatagtg cgcggttcat aaaattgcag attggcaaga
    14221 cacgatgccg atgagaactg ctctggcagc atgtaattga gcatcaccac atcagcttgt
    14281 ttgaggatct gcatctcgtt cacttctgcg cgtgaataat ccagcagaat ggtttgcttc
    14341 cccgccgccg ctttgtattt cgccagatta atcgccggct tagtcataaa cgaatcatcc
    14401 tgcggcaaaa caccgtcggg ctgaatttct ggcagccgca gttctttaag gaacatttcg
    14461 gcacgatgaa taaacgcgtc gtcactacag ccaaactggc gggcaatact cagcgcctgc
    14521 tggacgttgt aatacaccat atagctggtg aaggcgttat tattgacatg ttcggtatat
    14581 tcgtctggcc caataacatc atgaatttcc agacggtcgt taaccctcac cgcgcggcta
    14641 atccagaact ttgcagtttc cagaagtagc gccatgcctt catgcgcaat gaaactttca
    14701 tcccccgtgg tctgccagta ttgaataacc gcccaggcga tatcggccac cagatgatgt
    14761 tccgcctgcg ccgaggccac tttttgccgc agcccggtgc gaatgttaat ggcggcaaat
    14821 tccggcgtct cttcttcgcc gctgcgcgcg ctttcccacg gaaatagcgc gccctgccag
    14881 ccgttgcgtc gcgctttctc ctgcgcgcct ggcaagttgt gccagcgata acgcagtaaa
    14941 cttcgggcaa ccgtcggatc gctaaacaga tggaacggca gcaaaaatac ttctgtatcc
    15001 cagaaaacgt ggcctttgta gccttcgccg gttaagcctt ttgccgcaat actgctgcgc
    15061 tcgccgtgag ccggcgtcat gatgcgcaga tgataaagcg cataatctaa cgcttgctga
    15121 tcgtgcgcat cgccgccatt taccgtgata cgacgtttct gccaccattg acgccagttt
    15181 tctgttgatg ctgcaagatg ttggtcgtaa ctctgttgcg cgcacatttc aagctggcga
    15241 agcgacacgc tgccccactc gtctaaagca gcttgcctgt catcccgcca gtcgatccac
    15301 accagttttt gcaacgtcac tgtctcgcct gcatgaagct gcgtactggt atgttgcagt
    15361 aaacggcgct ctttggcggt atagcattgc tgcacatcac cgctcacctg gcagcaacaa
    15421 ctgatggcca catcactgcg cccatcctgg gtggtgtaga tcccctgcat caaatgctga
    15481 ccaaacaccc gcacctgggt ttcgtcgaga tgctggcgac cgtggttggt ttgcgtggcg
    15541 tcaatgcctg ttgaaatcag cactgacgcg tcggcgtcca gtggcataat agtgatttcc
    15601 agcgcaatga gcggcagttg gtctgccgaa acaaagcgac ggctggcgat ggtgtaacct
    15661 gcgccgttgc tggtacgcca gacaacgcta cggcgtaatt ctccactggc aaagtcaagt
    15721 tcacgctgcc aggcttcgcg ggataacgag aaaacctcac catttatggc aatctccatc
    15781 cccaagatat caggcagatt caccagttcg ttgatttcac cttttcccgc ccgatgatac
    15841 agccccgcca gatacatccc tcgcgtctgg cgcgtgtaat cttcttcatg gctggcgcga
    15901 agcccaagat agccgttccc ctgcgccatc agcgatgcat acttgttcag ggtatgctgg
    15961 ctgaaatggg gttctgataa cgttactggc ctggtcataa ttccacacgc gtccctgttt
    16021 ctgcggattg atataacgcc gccaccagtt gctggatgat gtacccctgc tcggcgtcgg
    16081 ctatcatcac gggcttgccc tgtacgtgat tgataaaggc ttccatgctg cgcaaatggc
    16141 ggttgtcgtc tgccatttcc cgttgcatca gcgtcattaa ttcaccgttg ttatcggtgt
    16201 agatatgtgc tggaaacagc gtcgcaccgg ctttatcacc acaaaagctg acgttcataa
    16261 tcaactgttc gcggatgttg agtgcaaatg acgtttccag ccacagaatg ccgccgttat
    16321 gaaattcaat ggtgccaaac agcgaatctt cgacgctgta agttgccgga tcccactcgc
    16381 caaattgacc acagctcttt tgcgtgccga tcttttgaaa actatgcgca ttcacgcttt
    16441 tcaccgccgg aaagcccagc acatacatcg cagcatccag catatgaatg ccgatgtcga
    16501 tcagcaggcc accaccctgc agttctttat tggtaaagac accccagccg ggaacgccgc
    16561 agcgacgcag ggcgcgggcg gtggtaacgt aaatctctcc caaaacgccg ttggtcacct
    16621 gttcacgcag ctgttgcgta tcgagcgcaa acgatggtga aagtcgtagg ccagcacctt
    16681 gcccagtttg cgcgcggtat cgcacatttc ccacgcctgt tctgtcgtca tggcgggcgg
    16741 tttttcgcac atcacatggc agcctgcttc cagtgccatc agggtatgtt cgtaatgaaa
    16801 acggttaggt gagccgacgc taaccacatc aggtttcacc gccagcagca tggcctgcgg
    16861 atcgtcccac acggatgcat tcccgtattt ttctgccagc gcctgcgcct gggaaaggcg
    16921 gctgtcacag acagccacca gttccagatc gttgcgggtg cagtagtacg aagcatgaac
    16981 cttatccgcc acctggcccg cgcctattat cgcgacccgc agcggagagc ttgtcattgc
    17041 acttttcaca aagacctctt agcaggtacg caaccaggcc aacgaatcac ggtacgcctg
    17101 ggcaggatct tctgcccgga tacgcccttc atacaccaca taaccctgat agttatcggc
    17161 gcgcagctgt tcaaacagcg cgtggaaatc gagggtgcca ctgcccggct ggtaacgatg
    17221 gttatcggca atatgcacat gaccgagcaa gtcacggttg tcgtgcagcg cctgcgccag
    17281 gttatcttct tcgatgttca tgtgatagaa atcgccgata atctgcacat gtttaagatt
    17341 gttttcgacg atgtaacgac gagcatcggc gagggtgttg atcatatgat cctgatagcg
    17401 gtttaacggt tcgagataca ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg
    17461 cagggaatca ctcaccagtt tgcggtcgcc gtccaggcta cgcggcgagg tcatcggcgg
    17521 taagcggaag gtaaacatgc cccacgctgc cggaaacccc ccacttctgc cagcgcctcg
    17581 agaatgcgtt cgatctgctt taagccatta agacgacgct cttcgataaa gtcaccaatc
    17641 cagccgtcat agccgccgca ggcggtggtc actggtaaac cggtttcttt gatcgccgct
    17701 ttgacttctt cgatgttgtt gaccagcagt ttgccgtcaa tctcaaaacc atcgaagccc
    17761 atctctttga tataacgaaa tctctcgcgg atattttccg gaaagaatgc ctgattctgt
    17821 gtgccgattt tcattttttc attcctttga attaaaacgt aacgcccatt ttgatgctct
    17881 gttccggatg ctgatcgaca tactgcatgt agctttccgg gctgttggca aaggtcacta
    17941 ccgggtcgat taaatcttcg caattgagat aaccgttcat cagcagttcc cagcaggttt
    18001 cttcaatacg cttacggctc cagcgcggat aatccgggtt cggttcgctg cacgcgcggg
    18061 agaaaacaat tttggcgtta ttgaaatgcg cttcgcgtcc gaggttaaaa ccttcagcaa
    18121 atggtttggc aaacgcgaca taggagatgg tgccgccgta ggccaggccg cgcagcgccg
    18181 attgcagcgc gtcggcgtaa ccgctggttt cgataatcac atcagccccc tgcttgccgg
    18241 tcagcgtttt gatctctttg cctacgtcag tgccaatggg gttaaggcag aaatcagcac
    18301 cgtggcgacg ggcaatatca cagcgatggg cgataggatc gacgccaatt accaccgatg
    18361 cgccagcgcg tttcgccagt tggatggcaa tttgaccgat cgcgccaagc cctaccacca
    18421 cgacaaaatc ccctacgcgt acgttggcat cgcgcacgcc gctcatggca aactgcgccg
    18481 ggtcgtaaca gacggcgttt ttccaggagc tgccttgcgg cattttgcgc agcttgtagt
    18541 tattcactgc gttaatgatg accgtctcgg agagcgggcc gtagccgcat acgctgtcgc
    18601 ccaccgcgta gtctgtaacg tcgctgccgc actcgataat gtcgccaacc accatgttgc
    18661 caagctggaa tttgccaaac tcaatgccgc gcggcgcatc tgccggacgc ggggtgaaca
    18721 tctgccactc gccgttaaag tcttcatcga taaacgggct ggcggcgcgg aagtcgacca
    18781 cttccgttcc gtgtttcggt gcgccgaaac gcacgcggat cttcacttca ttggctaaaa
    18841 tcgcccgatc ttcatactca accagcgcag caacacgcgg tgctgtggct actaactttt
    18901 tcatgtaaaa ctccttgtaa agaaactggc cgctttagcc cttcacgcca ccggcggtca
    18961 aaccactctt aataaaacgt tccgaaagcg catacataat caccaccggc aatgcggtca
    19021 ccagcgaggc cgccatcatc cgtccccaga tgtaatcagg cgtactgaac agcgcgttca
    19081 ggcccactgg taaggtgaaa ttgctggcgc tggagaggaa aatcgacgca aacagatagt
    19141 cgttccacgc caccataaag cagtagacga acaccgatat cagcccggac atcgccaggg
    19201 gcacggtgat acggaagatg atttgcagac ggttgaggcc atccatcatc gccgcttctt
    19261 cgatctcatc agggatggtg tcgaagtaac ttttcagcat aaacacggcg gtgggcaggg
    19321 tttgcgtcac catggtaatg atcagcgcca tctcggtgtc gtaaatacca agcgcggtga
    19381 tgattttgaa cagaggcacc accagcaaaa tgccagagaa catatacacc gtgtaaaagc
    19441 tggcgttgat ggtcatccgg cctttaaagc gcaggcggga aagcgcgtaa gctccgagaa
    19501 tgccgagaaa taccgccatc acggaggaaa ccaccgacac caccatgctg ttacggaagt
    19561 agtcgacaaa cgggaaaatc atcgggttaa aaatgtcgac gtaatgctcc agcgtccact
    19621 gttgcggcag cagcgtagga tgcagcgata tcgcctcttt cgcgcttttg aacgaggtca
    19681 tcagcatcac aaaaaacgga aacagcgtga tgatgagaaa cagcgccagc ccgcagtaaa
    19741 aaccgatacg actgagcgtg cgtttatttg ttgccattga ggttcacccg tttcctggtc
    19801 agcagaatga cagcgaaaat aatgatgaag agcaccaccg agatcgccgc cgctttccct
    19861 aagtcattaa aggcaaaggc ggttttgtag agatagacac caagaatatc gaccttggtt
    19921 gtcagcagat aaacatcagc gaacatgtag aacatccaga tggtgcgcag ggtcaccacc
    19981 gtcgccagga ccggcataat tgcgggcagc gtgacgatgc gaaaccgctg ccaggcatta
    20041 gcgccatcca tctctgccgc ttcgtacagc gatttgtcga tggtctgcaa aatcgccaga
    20101 aacgagataa aggcatacgg gaagtagcgc cagatggcga acagcaccac cagcacaaag
    20161 ctactgcccg gattatcaaa ccacagcggt gcctgctcat agagatgcag aagatcgacg
    20221 ccgaggtagt tcacaatgcc gtagccgttg ttgaacatgt atttccaggc aaacaccagc
    20281 gaaatggacg gcgttacgta ggagaggatc accagcgaac gcgcggtttt gcgcaggcgg
    20341 aactcgcggt tgaaaaacat cgccacggcc agcccgagca cggtgctgcc cgccaccacc
    20401 agcgccgtat accagaccgt catccacagc gagtgccaga agccgggatc ggagagaatg
    20461 cgcacatagt tactcacccc aacaaacgtt gactcgatgt tgggattgag cggcagacgt
    20521 aaaaaactga tttcgatatt cgacaccatc ggccacgcca ccagaccgcc cagcagtaat
    20581 aagctgggcg cgagaagcag cagcgcaaac ggcatatcgg aacgacctga aaaaagcctg
    20641 ttcattgcgg attcctgtcc tgggtttagt gctgttcgat cagttcatcc agctttttct
    20701 ggctcgcttg cagcgtgcca gggagatcgg ctttacccac ggtgacgtta tgcaccatgg
    20761 agctcaccac accagaaccc gtcacatcgc ccatgcgggt aaagttttta tcccctactg
    20821 cgccaaagac ctgaatattt ggcagttcac cgattagctg attcggtagt tcacccagcg
    20881 ccttaataac gtcgttgtct ttccaggtgg cggtagtgac aaccgcttta ttcaccggca
    20941 gcgcagcacc tggcgacatc atcacccagt cggcaatgtt gtccgcctgc tccataaagg
    21001 tgacaaattt ttctgctgct tcggtctttt gtccggcggt aatggtcagc gaggtcaaca
    21061 tgccgtagac cgcagagttt ttctcggttg gcaccacgaa accgacgttt ttcgggtcgc
    21121 cttctttaat cacagccgga aggatatagg tggagtaaat cgccatcggc gcggtgccgt
    21181 tcataaaggc gtctttcact tccatgatgt cgttagaacc cggcatggtg ttggcagcaa
    21241 ggtcgcggta ataggtcagc gcctgcatca tctctggtgt atcaagggtg attttgcctt
    21301 cggcgttaaa gacgttggcc tagttggata acgcaaactg ggagaaggat tgttccgtca
    21361 gcacgctttc tgccgtaggc agcgcaatac cgtatttttt attcgccggg tcattcagtt
    21421 tctgtgcaac gtccagcagc tgttgccagt ttttcggctc ctccagcccc gcttttgcca
    21481 gcacatcttt gcgataccag atgtcgccaa tccaggcgct gacaggaaca ccggtccatg
    21541 cgctaccatc ttcggtacgc acaatacgca gtacgccatc gtaaaacgcg ccttcaccaa
    21601 cgttgctgat gaccgtggca accgctttgc gatcgataag ctgctcttta tccataactt
    21661 tggcgtagtc atggctggtt tcgatcactt ccggcagcga gccgctacgt gaaagggtga
    21721 tgactttggt gttatgagcg tcttcttcca cgggcacttg tttaacggtg atacctgggt
    21781 tttctttttc aaaacgggca atcaatttgc tgataactgc ctgacgctcc tgctccaccg
    21841 aagaatgcat aaattcgatg gatacatggg ttttattttc ttctttacag cctgaaatca
    21901 gggcgcagga aaccagtgct gataacagca cgatttttga tttaatcatg agatgttcct
    21961 ttttatttaa tc
//
LOCUS       DAJTUN010000060        21888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTUN010000060 DAJTUN010000000
VERSION     DAJTUN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.060"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..409)
                     /gene="sxy"
                     /locus_tag="OO470_002171"
     CDS             complement(<1..409)
                     /gene="sxy"
                     /locus_tag="OO470_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6251408.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            628..1137
                     /gene="sulA"
                     /locus_tag="OO470_002172"
     CDS             628..1137
                     /gene="sulA"
                     /locus_tag="OO470_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6251409.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1493..2539
                     /gene="ompA"
                     /locus_tag="OO470_002173"
     CDS             1493..2539
                     /gene="ompA"
                     /locus_tag="OO470_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6251410.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2615..3067)
                     /gene="matP"
                     /locus_tag="OO470_002174"
     CDS             complement(2615..3067)
                     /gene="matP"
                     /locus_tag="OO470_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6251411.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3252..5012
                     /locus_tag="OO470_002175"
     CDS             3252..5012
                     /locus_tag="OO470_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6251412.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5081..5599
                     /gene="fabA"
                     /locus_tag="OO470_002176"
     CDS             5081..5599
                     /gene="fabA"
                     /locus_tag="OO470_002176"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6251413.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5669..5836)
                     /gene="rmf"
                     /locus_tag="OO470_002177"
     CDS             complement(5669..5836)
                     /gene="rmf"
                     /locus_tag="OO470_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6251414.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6092..6655)
                     /gene="pqiC"
                     /locus_tag="OO470_002178"
     CDS             complement(6092..6655)
                     /gene="pqiC"
                     /locus_tag="OO470_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6251415.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6652..8292)
                     /gene="pqiB"
                     /locus_tag="OO470_002179"
     CDS             complement(6652..8292)
                     /gene="pqiB"
                     /locus_tag="OO470_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6251416.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8297..9550)
                     /gene="pqiA"
                     /locus_tag="OO470_002180"
     CDS             complement(8297..9550)
                     /gene="pqiA"
                     /locus_tag="OO470_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6251417.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9680..11587)
                     /locus_tag="OO470_002181"
     CDS             complement(9680..11587)
                     /locus_tag="OO470_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6251418.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11599..13707)
                     /gene="rlmKL"
                     /locus_tag="OO470_002182"
     CDS             complement(11599..13707)
                     /gene="rlmKL"
                     /locus_tag="OO470_002182"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6251419.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13951..15060
                     /gene="ycbX"
                     /locus_tag="OO470_002183"
     CDS             13951..15060
                     /gene="ycbX"
                     /locus_tag="OO470_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6251420.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15057..15599)
                     /gene="zapC"
                     /locus_tag="OO470_002184"
     CDS             complement(15057..15599)
                     /gene="zapC"
                     /locus_tag="OO470_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6251421.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15773..16783)
                     /gene="pyrD"
                     /locus_tag="OO470_002185"
     CDS             complement(15773..16783)
                     /gene="pyrD"
                     /locus_tag="OO470_002185"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6251422.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16894..17633)
                     /locus_tag="OO470_002186"
                     /pseudo
     CDS             complement(16894..17633)
                     /locus_tag="OO470_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17599..18114)
                     /locus_tag="OO470_002187"
     CDS             complement(17599..18114)
                     /locus_tag="OO470_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6251423.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18122..18664)
                     /locus_tag="OO470_002188"
     CDS             complement(18122..18664)
                     /locus_tag="OO470_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6251424.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18762..19745)
                     /locus_tag="OO470_002189"
     CDS             complement(18762..19745)
                     /locus_tag="OO470_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6251425.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19736..>21888)
                     /locus_tag="OO470_002190"
     CDS             complement(19736..>21888)
                     /locus_tag="OO470_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6251426.1"
                     /translation="LEISVPQIAIKSNARGYVPPERWDEGINALFSGANSIHSSADSD
                     SGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGD
                     DYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQT
                     YVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLA
                     KYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISF
                     DATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHM
                     DGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGLFYLSGSQQTYWHTDQ
                     QDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTR
                     KKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGYNSEGKTANGSASMDY
                     KGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSLGETNVLIAAPGAENT
                     RVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDLENAVVNVVPTKGALVL
                     AEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAIT
                     VRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         5418 a         5738 c         5153 g         5579 t
ORIGIN      
        1 ccacctcccc gagaatcgct tccagatgaa aagacatatt gggcaaatct ttcagtcttt
       61 cccgggtatt gcgcgtgctt ttctctttca gcgctgcatc gagagaatat ttcgacagac
      121 gcaccagctt cagttgattt cgccatagac tttcatcaac ccgatagtaa ttgagggtaa
      181 cggatcggcc acacttttta tatgtcagcc agacaggcgg atgttttaca cagtactgtg
      241 cactttgctc acaagcccga agatacaact caccatcaga aaccatcgca aacaccgtgt
      301 cgtcaacggt caggctgtaa ctgccaaaca atgatcggta ttcaattgtg cccaacgttg
      361 ccaggtattc ttgtgattta tagatccgct tataggagag gcttttcata aaattccttt
      421 taaaatcata acataaaaga atgattcaca ttaacggatc cgttaactac gaaaataggc
      481 aacttattct taaggggcaa gattaattta tgttttcccg tcaccaacga caaaatttgc
      541 gaggctcttt ccgaaaatag ggttgatctt tgttgtcact ggatgtactg cacatccata
      601 cagtaactca caggggctgg attgattatg tacacttcag gctatgcaca tcgttcttcg
      661 tcgttctcat ccgcagcaag taaaattgcg cgtgtctcta cggaaaacac tacagccggg
      721 cttatcagtg aagttgtcta tcgcgaagat cagcccatga tgacgcaact tctactgttg
      781 ccattgttac agcaactcgg tcagcaatcg cgctggcaac tctggttaac accgcaacaa
      841 aaactgagtc gggaatgggt tcaggcatct gggctaccct taacgaaagt aatgcagatt
      901 agccagctct ccccttgcca cacagtggag tcaatggttc gcgctttacg cacgggcaat
      961 tacagtgtgg tgatcggttg gttgacagat gatttgactg aagaagagca tgctgaactt
     1021 gttgatgcgg caaatgaagg taacgctatg gggtttatta tgcgtccggt aagcgcatcc
     1081 tctcacacaa cgagacaact ttccgggcta aaaattcact ctaatttgta tcattaagta
     1141 aatttaggat taatcctaga actttttttg tcgcccagcc aatgctttca gtcgtgacta
     1201 attttccttg cgaaggcttg tctgaagcgg tttccgcgtt ttttttctgt aaattgtcgc
     1261 tgacaaaaag attaaacata ccttatacaa gacttttttt tcatatgcct gacggagttc
     1321 acacttgtaa gttttcaact acgttgtaga ctttacatcg ccaggggtgc tcggcataag
     1381 ccgaagatat cggtagagtt aatattgagc agatcccccg gtgaaggatt taaccgtgtt
     1441 atctcgttgg agatattcat ggcgtatttt ggatgataac gaggcgcaaa aaatgaaaaa
     1501 gacagctatc gcgattgcag tggcactggc tggtttcgct accgtagcgc aggccgctcc
     1561 gaaagataac acctggtaca ctggtgctaa actgggctgg tcccagtacc atgacactgg
     1621 ttttattcct aacaatggtc cgacccacga aaaccaactg ggtgcaggtg cttttggtgg
     1681 ttaccaggtt aacccgtatg ttggttttga aatgggttac gactggttag gtcgtatgcc
     1741 gtacaaaggc gacaacatca acggcgcata caaagctcag ggcgttcagc tgaccgctaa
     1801 actgggttac ccaatcactg acgatctgga catctacact cgtctgggtg gtatggtatg
     1861 gcgtgcagac accaaggcta acgtacctgg tggcgcatcc tttaaagacc acgacactgg
     1921 cgtttctccg gttttcgcag gtggtgttga gtacgcgatc actcctgaaa tcgctacccg
     1981 tctggaatac cagtggacca acaacatcgg tgacgcaaac accatcggta ctcgtccgga
     2041 caacggtctg ctgagcctgg gtgtttccta ccgtttcggt cagggcgaag cagctccggt
     2101 agttgctcca gctccggcac cggaagtaca gaccaagcac ttcactctga agtctgacgt
     2161 tctgttcaac ttcaacaaag caaccctgaa accggaaggt caggctgctc tggatcagct
     2221 gtacagccag ctgagcaacc tagatccgaa agacggttcc gtagttgttc tgggttacac
     2281 tgaccgcatc ggttctgacg cttacaacca gggtctgtcc gagcgccgtg ctcagtctgt
     2341 tgttgattac ctgatctcca aaggtatccc ggcagacaag atctccgcac gtggtatggg
     2401 cgaatccaac ccggttactg gcaacacctg tgacaacgtg aaacagcgtg ctgcactgat
     2461 cgactgcttg gctccggatc gtcgcgtaga gatcgaagtt aaaggtatca aagacgttgt
     2521 aactcagccg caggcttaag ttatcgtctg gtagaaaaac cccgctgctg cggggttttt
     2581 tttgccttta gtaaattgaa ctgactttcg tcagttattc cttacccagc aatgcctgca
     2641 gatcctgctt aagagaagac attttattcg cgtatttctc tttgttttcc gcatcttcaa
     2701 tcagctgaac aatcgtttca gaaagcgttt taccgcgacg ctgtgcaaga ccagccagac
     2761 gttgccagac aataaattcc agatcgatcg attttttgcg cgtatgctgg tgttccgcat
     2821 taaaatggcg cttccgtctg gcccgaatgg tctgcttcat gcgattgacc agttccggat
     2881 tcatatgctt gtcaatccag ccatttacca gcacgggttc attttcgagc gagagcaaca
     2941 catcgacggc ttcctgggcg gcactggctt ctatgtaacg ggtaattaac tccccttcgc
     3001 gatgcttctt caccagatac ttccatttcc aaccgctttc aagattttca agttgttgat
     3061 atttcatagc gatctcaatg ttaccgtgta actctttaca gaatatcagc tttttaggcc
     3121 agtgaagaaa agaatctcca tcctgtgagc tttaacgccc agccaggagt attgtcggtg
     3181 ctttctacgt gtgctgtagt gccggttacg gtataatcgc ggctttgaca acagactaaa
     3241 aaacatcaac tttgaccatt acgaaacttg catggcgtga cctggttcct gataccgata
     3301 gctatcagga aatatttgct cagccacatt tgattgacga aaacgatcct ttattcagtg
     3361 atactcaacc gcgactgcaa tttgcgctgg agcagttgct gcatacgcga gcatcctcct
     3421 cttttatgct ggctaaggcc ccggaagagt ctgagtatct gaatcttatt gccgatgccg
     3481 cgcgtacgct acaaagcgat gcaggccaac tggtgggcga tcactatgag gtttccggcc
     3541 actccatccg cttacgtcac gcagtgagtg cagatgataa ttttgcgact ttaacgcaag
     3601 ttgtcgctgc cgactgggta gaagcggagc aactctttgg ctgcctgcgc cagtttaatg
     3661 gcgacattac cctgcagcct ggtctggtgc atcaggcaaa tggcggtatt ctcatcatct
     3721 ctttgcgtac actgctggcg caacctctgc tgtggatgcg gctgaaaaat atcgttaacc
     3781 gcgagcgttt tgactgggtt gcgtttgatg agtcgcgccc tctccccgtc tctgtgcctt
     3841 cgatgccatt gaagctgaaa gtcattctgg taggcgaacg cgaatcattg gctgatttcc
     3901 aggagatgga gccagagctt tcagagcagg ctatttatag cgaatttgaa gatactctgc
     3961 agattgtcga tgcggagtca gtaagccagt ggtgtcgctg ggtgacattt accgccagac
     4021 ataatcactt acctgccccg ggagcggatg cttggccggt acttatccgc gaagcagcac
     4081 gctacaccgg tgaacaagaa acacttccgc ttagcccgca gtggatcctc cgccagtgta
     4141 aagaggtcgc ctccctgtgt gatggcgaca ccttctccgg cgagcagcta aacttaatgc
     4201 tgcagcagcg tgaatggcgc gaaggtttcc tcgctgaacg tatgcaggat gagatccttc
     4261 aggagcaaat cctgattgaa accgaaggcg aacgcatcgg gcaaattaac gccctttcgg
     4321 tcattgaatt tccgggtcat ccacgcgctt ttggcgaacc ttctcgcatt agctgcgttg
     4381 tgcatattgg cgatggtgaa ttcaccgaca tcgaacgcaa agcggagctt ggcggcaata
     4441 tccatgcgaa agggatgatg atcatgcagg cgttcctgat gtcggaacta cagcttgagc
     4501 aacagatccc cttctcagca tcgctgacat ttgagcagtc atacagtgaa gtggatggcg
     4561 atagtgcctc gatggctgaa ctctgcgccc tgataagcgc cctcgctgat gtgccagtga
     4621 atcagagtat cgctatcaca ggttcagtcg atcagttcgg ccgcgtccag ccggtcggtg
     4681 gtttaaatga gaaaatcgaa ggcttctttg ctatttgcca gcaacgtgag ttaacgggga
     4741 aacaaggtgt cattatcccc actgctaacg ttcgccattt aagtcttcac agtgaactgg
     4801 tgaaagcggt agaagaaggc aaattcacca tctgggcagt agacgatgtg actgacgcac
     4861 tgccgttatt attaaatctg gtgtgggatg gcgaaggcca aacgacgctg atgcaaacca
     4921 tccaggaacg tatcgcgcaa gcatcgcaac aggaaggacg tcaccgtttt ccatggccat
     4981 tacgttggct gaactggttt attccgaact gatcggactt gttcagcgta cacgtgttag
     5041 ctatcctgcg tgcttcaata aaataaggct tacagagaac atggtagata aacgcgaatc
     5101 ctatacaaaa gaagaccttc ttgcctctgg tcacggtgaa ctgtttggcg ctaaaggccc
     5161 gcaattgcca gcaccgaaca tgctgatgat agaccgtgtg gtcaaaatga ccgaaacggg
     5221 tggtaacttc gacaaagggt atgttgaagc agaactggat atcaatccgg atctgtggtt
     5281 cttcggatgc cacttcattg gcgatccggt tatgccggga tgcctgggcc tggacgcaat
     5341 gtggcagctg gtagggttct acctcggctg gctgggcggc gaaggtaaag gccgcgcgct
     5401 gggcgttggc gaagtgaaat tcactggtca ggtactgccg acagcgaaaa aagtgaccta
     5461 ccgtattcac tttaaacgca ttgttaatcg tcgtctgatt atgggtctgg cagatggcga
     5521 agtgctggtt gattgtcgtc tgatctatac cgccagcgac ctgaaagtcg gtctgttcca
     5581 ggatacgtct gccttctgat tatcatcaga cagagtatca aaaggcgaaa cctccgcaat
     5641 gcggaggttt ctttttaaag agacagaatc aggccattac taccctgtcc gccatggctt
     5701 ctcgccagcc tcccagccat tgtgaccttt gactcagcgt ctgataggga cacatttctt
     5761 ttgagcgtcc ggcgatgccg gcctgataac cacgttgatg tgcccgttcc aggcgatctc
     5821 gtttttgtct cttcatgcct cgtttccctc atactggttt ctggtggaaa agaaaacagt
     5881 gattactaaa tgtgcaatca cagttaagca ataccgcgaa tcataactgc cgtcaatgcg
     5941 caaaattcac gccactgtca tatttgtgag ctacacgggc atgtcggttg tacaaaaaat
     6001 gtgaagagga acaaattttt ccgcacgtta gaaacaaaaa aaccgcctca ggcaacatac
     6061 cggagacggt tatatttaca aatctttata attaaggtag acgctttatc tcttgtgcaa
     6121 tagaagcggc ttcctgactc cagacaccgg ccagcacttt aaccatctca tcgtaaccat
     6181 cctgagtttg cactccttcc agacggaacg gacgtttgat cagttgtccc tggtggttca
     6241 acagccactc accactgaca atgaccttgc catcatagcg accgttaaac tccgttacgg
     6301 taacattgag cgtgtcctgg gcgcttccca gaggctggga ggcaaccacc cagccgggca
     6361 gttgcgtact caggttggca accagggtgt tgcgcaactg ttgatccaac gggctggccc
     6421 acaagttgtt gttggcaatc acatacttca catcactggt ttgataaacc acaccattcc
     6481 ccgccagata gtcaggaaca gtgacctgct ctacccataa cagacgattg ccctggctgg
     6541 cggtactttg tgtaccgctc tgcaccacag gtaactggta atagttttta ttaatttcgc
     6601 cggagctgca tccggccagc cacagtgctg caatcgtcac tagccacttt ttcattgttt
     6661 cgccctcttc ggctctggat cttttttgtc cttcgcttca aataccagcg cgttactctt
     6721 ctcattgagc gttttcagca ccggttgcag ttctcgcaac acctgatcaa ggcgctgcat
     6781 atccgccacc atcttgttgt aggctgcgga gccaggctgg aagccctgca tgctgcgatt
     6841 caattcacgc aacgttgact gcatatccgt cggcaactgc tgcatcgact ggctggcgag
     6901 gatcttgttc atgctatcca gcgtcgtttg caggtttttc attgtgcgct gactttcaga
     6961 aagcgtactg gttgcctgtt caatcatcgg attcaatggc agtttgttga tcttatccaa
     7021 cgcttccatc agtcgttgct ggatttgcgc caagccgccg ctaacagtcg ggataatctg
     7081 ataaccatta aattcacgaa taccggttat tgcaggcgta tttggataga aatcgagatc
     7141 aacatacagt gcaccagtga ccaggtttcc ggttttcagc gatccgcgta aaccacgttt
     7201 caacaattcg ccaaggtgct caacaacatc cgcattttcg ccaagctgca ttttcagccg
     7261 ctctggctcg atacgaatca gtaccggaat acggtaatca tcgttaaatg tctgacgcat
     7321 attcggcgca aagaatggca ctttgcttac ggtacccagg cgaatacccc ggaattctac
     7381 cggagctccc ggttgcagac cgcgtaccga atctttaaag aacatcagat aatcaatgtg
     7441 atcggtgtac aacgaatcct gaatgctctt ctgatcatca tacaaaacga aagctgtttt
     7501 cggtgccact ggctgcccta aatccagacc ttccggcaca tcaaagctaa caccgccact
     7561 aagcagcgtt gtcaatgagc ccatctccac acgcatcccc gctgacgtca gatcaaccgc
     7621 aatgccacta tctttccaga agcgaacatt gctggtcacc agtcggtcat aaggtgcatt
     7681 gatgaacagt tgataactga tattgcgttt ttgcgtatcg aaggtgctgg tttcaaccga
     7741 acctacccga tagccacgga acagcaccgg atctcctggc gagagctgcc cggctttttt
     7801 gctatcgaga atcacacgga tacctttcgc atcaggcggg gccaacggtg gtgagtccag
     7861 caaatcgtat ttatccattt tgctgccttt cgcgcctggc tgcagttcga tataaactcc
     7921 agacagcagc gttcccaggc cgctaatccc ttcgcgacca atctgcggtt tcaccaccca
     7981 aaagacggtg tctttatgca gcaatttttc cataccggaa ttcagccgcg ctttgatttc
     8041 aacgtgcgtc aaatcatcag ccagtgtggc gctttcaacc acgccgacgt caacgctacg
     8101 gcttttaatg gtggttttgc caccttcaat tccttccgca ttcgcggtga tcagggtcac
     8161 ttccggtccc tgatggctgt aatgataaaa aagaacccag gccccaatga gcgccgtgac
     8221 gataggaaat atccacacgg gagaccagtt cttcactttc tggattttgg cttccccatt
     8281 attagattcc atactgtcag gactcctcat gctctgattc aggttgacga tcccacgaca
     8341 aacgcgggtc aaacgtcata gcagaaaaca ttgtcattat gacgactaaa gcaaacatta
     8401 atgcacccat tgccggataa atactcatta aacctcccat acgcaccagc gccgagagca
     8461 ccgcgataac gaaaacgtca atcatcgacc agcggcctac aaactcaaca acttcataaa
     8521 tcaaatgcat tctttcactg tcgcgcttgc catgcccttt ggcatcccaa cacagccacg
     8581 cgatggcgat catctttaac gttggcacca taatactggc cagaaagatc accgcagcga
     8641 cgggataaga gccttcgctc cataacagaa tgaccccagc gagaatcgtc gacggcatct
     8701 tcgagcctaa taaatccgtc accatgatgg gcaaaatatt agccggaaga tacagcatga
     8761 tggacgttac aagcagcgcg agtgtccact gcaggctgtt tctacgccga acgtaccctt
     8821 tggtactaca acgcgggcac acgggttcat cagcaggaag gattgccgta caacaggagc
     8881 acgaacgcag cccctgacgt atccccgtga cgcctggttt tagcggctgg cgcagttctg
     8941 gcatcggggc gatgtcgtcc cataaccagc gacgatcaac gcactgaaaa gcgcgcagtt
     9001 gcaggacaca aaataaacac caggggagaa agctgctgcc taccccaatg ctgccgtaag
     9061 ccatcagttt aacgaaactg accagcacac ccgcgaggaa aatctccgcc attccccagg
     9121 ttttgagttg aaaaagcacc cgtgccagtt gctcttttaa acggaccggt aattccgcgc
     9181 gattcaccag taacagaatg gttatcagac aaaacgcggg aaccagttgc acaaacaaca
     9241 ggaaaaaggt gccgaggctg gcgtagtcct cagaaaaaag cacgccggga atttccagta
     9301 atgtaatttc actggtaact cctgcaacgt tcatattcac aaaaggaaac aagttggaca
     9361 gcaacagcat gaacagtgca gccaacgcat aggcggtggg acgctgccga ggggcatccc
     9421 acgccacggt taacgttgtg ccacaccggg gacatgccgc tttctgacca tgctcaaggc
     9481 gcggtaacgc caccagcatg tcacactgcg agcacaggat gtgcttcgcg gcatgatgat
     9541 gttcgcacat tggtactcct taatgatgcg ctattttaca gatgactaac aaagtgttgg
     9601 ttttcatgcc tgataaaccc agttcatcag gcaattttac cgttgtcagc tgtctctgtt
     9661 taaatcgact attttgcgat cagccaccat tttttaacgc ttcaagatac tcccagcgtt
     9721 caaaggcttg ctccagctcc tgctctgcag cagccatatc agcaagcact ttttgcgtct
     9781 gctcatgcgg ctgactgaag aaggaagcat ccgccacttg cgtctgtagg gcttccagct
     9841 tcgcctccag atcttcgagc aattgcggta gctgctccag ttcgcgctgc aatttatagc
     9901 ttagtttgct actgctgcgt tttacagttt ctgcttttgg cgcggcggct tcttcgcttt
     9961 ttttcaccgc aggctgtttg agtgccacat attgttcttg ctgaccacgg gcatcatgat
    10021 aaccgccaac ataacgacca attttaccgc cgccttcgaa gatccagcat tctgtaacgg
    10081 tgttatcgac aaactgacga tcgtggctaa ccagcaatac cgtgccctga tagctgtcga
    10141 tcagttcttc cagcagttcc agcgtttcga catcaagatc gttggtcggt tcgtcaagaa
    10201 tcaataagtt gcttggtttg aggaacaaac gcgccagcag caagcggttc cgctcaccgc
    10261 cagaaaacgc acgtactggc gtcatcgccc gtttcggatg gaacagaaag tcctgcaaat
    10321 agcccaatac gtggcgtggc ttgccattaa ccatcacctc ttgcttacct tcggcaaggt
    10381 tatccatcac cgttttatcg ggatccagtt ccgcgcggtg ctgatcgaaa taagccactt
    10441 ccagtttggt gccaacgtga atacgcccgc tgtccgcttg aagctgaccg agcatcagtt
    10501 tcagcagcgt ggttttgccg cacccattcg gaccaatcag ggcaattttg tcgccacgta
    10561 gaacctgggc agaaaaatct ttcaccagtt gcttaccgtt aacctggtag caaacgtctt
    10621 ccatttcgaa aacgatctta ccggagcggc tggcctcttc cacctgcatc tttgcggtac
    10681 ccatcacttc gcgacgttca ccacgttcgc gacgcatcgc tttcagggcg cgtacgcggc
    10741 cttcattacg ggtacggcgt gctttgatcc cctggcgaat ccacacttct tcctgcgcca
    10801 gtttgagatc gaactcggca ttttgtagtt cttccacgcg cagggcttct tctttttcct
    10861 gcaggtactg gtcgtaattc cctggatagg tcaccagctt gccgcgatcg agatcaacaa
    10921 tgcgcgtcgc catattgcgg ataaacgaac ggtcgtggga gatgaaaata atcgtcccat
    10981 tgaaggtttt aaggaacccc tccagccagt cgatggtttc gatatccaga tggttagtcg
    11041 gttcgtccag cagcaatacg cgcggattgc tcaccagcgc gcgtcctaat gccgctttac
    11101 gcaaccagcc gccggaaagc gacgacagcg caacgttagg atctaacccc agttgcgcca
    11161 gcacttcgtt gatgcggttt tccagctgcc acaggttgtg gtgatccagc tgttcctgaa
    11221 ccttcgccag ttcgttgaga tttttctcgc tcgggtcgtt catcaccagg cgcgaaatat
    11281 cgtgatagcg tttcagatat tccgcttgtt cttcaatgcc ttcggcaacg aaatcataaa
    11341 cgctaccctc aacgttacgc ggtggatcct gttgcagacg agctacaatc aaatcttgct
    11401 cataaataat gcgaccgtca tccagccctt gttcacggtt gaggattttc attaacgtcg
    11461 attttcctgc gccgttgcgg cccaccagac aaacacgttc gttatcttcg atatgcagtt
    11521 ctgcgttatc gagaagcggc gcgtcgctga acgacagcca tgcgccatgc atactgatta
    11581 atgacattac tattcctttc aggctgcggt aatcagccag cagttgtgga tctggcggtt
    11641 acgggcgaaa tcctgggaga gcgttttttg cgtaatttct tgtgctttca gtcccagttt
    11701 tgccaggccg tcgagatcca tacggaagcc acgtttgttg ttcgagaaca tgatcgtccc
    11761 acctgcacgc agcagacgtt tcaaatcttt catcagcgcc agatgatcgc gctgaacatc
    11821 aaacgcatct tccattcgtt ttgagttaga gaaggttggc ggatcgataa agatcagatc
    11881 gaactgttca tttgcctcac gcagccacgc caggcaatcg gcctgaatca ggcgatgcgc
    11941 acgcctagtc aggccattca gacgcaggtt gcgttctgcc cattccagat aagtacgcga
    12001 catatccacg gtggtggtgc tgcgtgcacc gcctaatccc gcatgcacgg tggcgctgcc
    12061 ggtataagaa aacaggttga ggaaatcttt gcctttgctc atctgaccga gcatacggcg
    12121 ggcgatacgg tgatcgagga acagaccagt atcgagataa tccgtcaggt tcacccacaa
    12181 gtgagcgtta tattcggtaa cttcaagaaa ctcgcccttc tcgcccagtt tctggtactg
    12241 gtttttgccc ttatggcgtt cacgggtttt cagcaccagt ttgtttggcg caatccccag
    12301 taccgaaatg gttgcagcga taatatcgaa cagacgctga cgcgctttgt gcgcatcaat
    12361 agtttttggc ggcgcatact cctgcaccac cacccagtcg gcataacggt caacggcaac
    12421 gttatattcc ggcagatcgg cgtcatacag gcggtaacat tcaatccctt cctggcgtgc
    12481 ccacttctcg aattttttga ggttcttacg caggcggttg gcgtaatctt ccgctaccat
    12541 tgccggttta ctgtctggtg tgctttcggc aacgtggtaa tttttctgta cgcagtccag
    12601 cgggccgttt ttcgccttgt actgtttgtc tgcacgcagc tgcaggcagc ttagcagatc
    12661 cggcgaggca ctaaacaaag agagattcca gccaccgaac tggtttttca tgattcggcc
    12721 cagcagacta tgcagcgcaa tcagcgccgg ttcgctgtcc agacgttcac cgtatggcgg
    12781 gttgctcaac actgtaccgt acggcccttt cggcagcgga ttggtcagtt gcgcgacatc
    12841 tttcacctca aaggtgatca gttcaccaat ccccgcaaga cgggcgttag tgcgcgcacg
    12901 ttgaatcacc cgtgcgtcgc tgtccgaacc gtaaaaatga gagctatact cagccaggcc
    12961 tttacgggcg cgagtttgcg cttccgcttt cacttcctgc cagatagctt catcatgctg
    13021 cgcccagccg ctaaagcccc aacgcccacg gtgcaagcct ggtgcgcgat cggtcgccag
    13081 catcgccgct tcaatcagca acgtaccgga accacacatc ggatcgagca gcggtgttcc
    13141 tgactgccag ccggatcgca tcacaatagc ggctgccagg gtttctttga tcggcgcaat
    13201 accagcacga tcgcgatagc cacgcagatg taaaccatca ccactcagat caagagcgat
    13261 actggcggtt tctttatgca gccagacgtt aacgcggata tccggcgcat cgcgatcaac
    13321 atttggacgc ggcagatttt tccgcgtgaa agcatcgacg atcgcgtctt tcactttcat
    13381 cgcgccgtac tgactgttgc ggatggtgtc attcaaacca ctgaagtgga cagcgaaggt
    13441 cgcgccagga ttaaacatct ctgtccagtt gatcgcctga acaccgagat agaggtctaa
    13501 atcgctgtaa accttacact cgcccagcgg caacataata cgcgaggcca ggcggctcca
    13561 catcaggctc tggtaaacaa gccgtgtgtc gcccttgaaa tggaccccac cctgaaccac
    13621 ctggcattca acggccccca gattttccag ttcagttttt aacagctctt ccagcccacg
    13681 ggccgtactg gcaaacagag aattcatatc gtcactttta ctctaagagc ctaccgcagt
    13741 aggcgtaatt gttgcagtca gtctggacat ggagagcgcg gagaaaccgg agcgtacaca
    13801 agtacgtgag gatttcgagc acaacccagg gccaaactga caaataaaat agcctaacgg
    13861 gacaggcgct aagaaaattg ttgcgcatta tagctaataa gcgcgtcatg tcataaagtt
    13921 gagggcttat tttcatttga ggaccgcacc gtggcgacat taacccggct ttttattcat
    13981 cctgttaaat cgatgcgcgg cattggtctt acacatgctc tggctgatgt cagtggtctg
    14041 gccttcgatc gcatctttat gatcacggaa cctgacggta cgtttattac cgctcgccag
    14101 tttccccaga tggtacggtt tactccttca cctgtgcatg atggcttgca tctcaccgca
    14161 ccagatggca gtagcgcata tgttcgtttt gctgatttcg ccacacaaga cgcaccaacc
    14221 gaagtttggg gcacacattt taccgcgcga attgcgccag acgcgatcaa caaatggcta
    14281 agtggatttt tctcccgcga agtgcaatta cgctgggtgg ggccacaaat gacccggcgc
    14341 gtgaaacgcc acaacactgt acctctgtca tttgctgatg gctatcctta ccttcttgct
    14401 aacgaagcct cgttacgtga tctccaacaa cgttgtccgg ccagtgtaaa aatggagcaa
    14461 ttccgcacca atctggtggt ttccggcgcg tcagcttggg aagaagatag ctggaaagtg
    14521 attcgcattg gtgatgtggt gtttgatgtg gttaaacctt gtagccgctg tattttcacc
    14581 accgtcagcc cagaaaaagg gcaaaaacat ccggcaggcg aaccattaaa aacattgcaa
    14641 tctttccgca ctgcccagga taacggcgat gtcgattttg gtcagaattt aattgcccgt
    14701 aatagcggcg tgattcgcgt tggcgatgag gtggaaattc tggcaacggc tccggcaaaa
    14761 atttacggcg cagctgccgc tgatgatacc gccaacatca cgcaacaacc ggacgcaaat
    14821 gtagatattg actggcaggg acaggcattt cgtggaaata accaacaagt gttgctggag
    14881 caattagaaa atcagggaat tcgtatccct tattcttgcc gcgcgggcat ttgtggaagt
    14941 tgccgtgttc agcttttaga aggcgaagtc acgccgctga aaaaatcagc aatgggcgat
    15001 gatggcacca ttctttgctg tagctgtgta ccgaagactg cccttaagct gacgcgttag
    15061 actgcctgtt cgaggctgaa gctgtcaaca ttcacctgcg gtttcagcct gtcgttcatg
    15121 attttaatgg cgtcgcccaa ctgcatcgcg cgacccgcaa taacaacgca gggttgtgcc
    15181 agcaggcata gcgcggcatt ttcgccagat tcaacgacca gcaaattgac ctgctcgtga
    15241 gtatcgctca accaaacgca ggccgcatcg cccggcatgg gcacccacat ttcaccatgg
    15301 gagacaaaat gccagctttt cggcatttgc ggtttgagat aacgaattgc taccagtgca
    15361 ttcagcacca gttcggcttt ttggtcttta gataaattaa aatcgcggca cttttcttca
    15421 aaagagaaat agagcgcggc atcatcaaca caaaagcctg cgggagaaaa ggcatctgga
    15481 gtcaacattt tgcgcgcaaa acgtgagcga aatagcatac cattggctaa atcgagcatc
    15541 attcgatcgt gctcttcatc gtaataccaa cgccagttat cgtctggttt aattcgcatc
    15601 tgcctctccc ctaccctata aaaccgcagc aacacaatcc attgtcgctt cgcttaataa
    15661 cctcaacaat aaaagtacaa aatgtctaaa taagcaacaa cgatgaatat aaaacaacca
    15721 gggctgaaaa taaagccctg attatcttat aaagaggaat tcgaagaaat aattagatat
    15781 gggtaacgat ttctttaatc agcggcggac ctttaaaaat aaaaccagaa taaatttgca
    15841 ccagtgaggc acccgcagca atcttttcac gcgcagcgat aaccgagtcg atgccgccaa
    15901 caccgatgat cggtaagcga ccgtttaatt ctagtgacaa gcggcgaata atttctgtgc
    15961 tttttaactg aagcggacga ccacttaagc caccggtttg atcgcaattt ttcattccct
    16021 gaaccagaga acgatcgaga gtggtattgg ttgcaataac gccatcaata ttatggcgaa
    16081 ctaaactatc ggcaacctgg atcaattcgt cttcagaaag atccggcgcg atcttcactg
    16141 cgatcggcac atatttatgg tgcatcgctt gcaaatcatt ttgcttattt ttaatcgcgg
    16201 ttaagagatc atccagcgct tcaccatatt gcagcgtgcg taatcctggg gtattcggcg
    16261 atgaaatatt gatggcgata tatcccgcat aggcatagat tttttccata caaatcagat
    16321 agtcatcttt gccctgctcc actggcgtat ctttattttt gccgatatta atacccaaga
    16381 cgccgtcata atgggccttt tttacgttct ctacgaggtt atcaacgcca agattattaa
    16441 agcccatacg gttgatcaaa ccttcggcat ctaccagacg aaagagacgc ggcttgtcat
    16501 taccaggctg tggacgtggc gtgacggtac cgatctcgat cgatccaaat cccatcgcgc
    16561 ctaacgcatc aatgcactcc ccgtctttat caagaccggc tgccagacca agcggatttt
    16621 taaacgtcag gcccatgcag ttaacaggtt tcgcaggcac tttctgccgc accagtgctt
    16681 caaacggtgt tccggtaatg cggcgtaatt gctgaaaagt aaactcatga gcgcgctctg
    16741 gatcgagctg gaaaagggct ttacgaacga aggggtagta catgaactct cctggattcc
    16801 cggtgtgcaa accgggggcg tattatgtgc gagttcggac caaaagggaa ttgacctgcg
    16861 gcaaaaaaaa agcaaacgtt ttcagaacct gtttcagggc agattttgct ccttctttgc
    16921 actcaacccg ccgtaatcat ttatcgtccg ccaggcgaca tgacacggtg ctattacctg
    16981 acaccagttt gtctgacgct ggcttttagg cggcacgact cccgcattac ttaacgcttt
    17041 accatttacg ctcacctgaa tcaggttaat gtagtaaggc gttgggttag tgagttgtac
    17101 accctgcgaa ttccgtgtcc agcgtaattg ttgataagct tccagcgggt cgcccggcaa
    17161 accttcgggt cgccagaaca gtttaaatac cgagcgcatc gccactttta cgctctgatt
    17221 ttcaactttg ccggatggca cgctggcaat gcttaactca aatagcgttt cgcgatccac
    17281 aggcaagatg tcgctttccg ttctcagcaa gcgcaacgtg cctgtagtac cgggcttcag
    17341 gagaataagt ggcggagcgg ccagtaatag tgctggcact gtcgacgctt cccccccccg
    17401 gcccaacgcg ttgggcgatt gattttgctg ttaataagcc aagattcctg actggtatta
    17461 gtcagtaaaa tagatatcga ttctctgtct gccggaaaga taaatcgcgt tccgccgacc
    17521 accactcctg cctgcaattc acccgccacc agcagcgata aaacggcgaa aatcaagagc
    17581 gctaatctgt tccacgtatt agtcataatg aagtacaaat gtcgcgctgg cattcacttt
    17641 acccgactgc acatcgctat tgacaggtcg caattgcgca taaaaagtta atggcacctt
    17701 accgctatgt aggctgtagt ctttactcgc ctggttaagc ggaatcaata tcttcgcatc
    17761 atccagaatc gcaattgcca gcccactggc tgcatccgta ccactttcaa tggacagcat
    17821 ttcgctctgc actccgtcgg gggttcctga aaaggtgaca tcaaccgtgc tgacatctgt
    17881 tccgcaatcc tgcaaattaa tgatgaaaga tttctccggg ccataattcc catgaaaaaa
    17941 acgtgtcagc gagacatcgc ctaactgcac attgatttgc ttgctatcct gtgccagcac
    18001 gcatgtatta cggcgaaacg atccgctaat gttgaccgtg ccatcatggg caaatagctc
    18061 acagcccccc gttaatccca gtaagaacag aatccagatt atccgtttca acatcactcc
    18121 ttcactggta aaccagatca aaatagagaa ccgccgtcgc attaccgccc gttgctcccg
    18181 cctttgtgga cttataacga agttgatatt tgagtgtgtt atccccggct tttaagggcg
    18241 taagaggctg gacctgattg agcgggattt cttgctgact ttgcgcatca agaatttgca
    18301 cggcaatccc cgttgccata ccaccgcttc cgcctgtgtc agttagtttg agcagtgtcg
    18361 gcgcaagggt gtcagcctcg ccgctaaaaa ggacgtccgc gccagtaata ccagcggcac
    18421 aaccggtgat atcaatattc aaatcagcag cagtgcttac ggtattcgcg gcctgaaaat
    18481 cactgatatt aaaataacca atatgaatat tttgtaatgc acttttggtg gcgacatcac
    18541 agccctgatc gaccacattc ccataaatat gcatactgac ttgttcaggc ccagccatcc
    18601 cgacatggat gttaagaagg ctaaagaaca aaataacgca ctgatatatc gtttttttct
    18661 tcatacgttc acgcgttaat caaaatttat tctcaccgtt gccgtggctt caaatcgccc
    18721 ggtttctggt ggtacgcctg tcgtgctgac tggatagaca ttcattcgcg gatactgtct
    18781 gcatcatcag gaatagagag cggtagtgtc ccaccagcag gggaaataat gttgttctgg
    18841 ctatcggtca ccaccacgcc cacgccagga cgtgacgtct taatcgcctg gggatagctg
    18901 gggtctgagg ttgccgtcag actcagattg acgttaacta accctgcgtt tcccgtgtta
    18961 cacctgaccg gaattgcaag ttctgccgtt ctggccccaa gaggcttatg ccctacaaca
    19021 cgaaagttag ccgcaaatac gtcgcccaga tcaacctcga ttgtttctcc ggcattgacg
    19081 gaacatgagc caagtgaatt gatcgtacca gacaggtaat acactaaaac cggtgtacca
    19141 gtagtcgtgc aggaatcacc tgcgggtatg ttgtagcatt caaataatcg cgccagcgcc
    19201 ataggaggaa tgacgaccga accaacgaac ggctgcaata ttttcaggga aaagctaccg
    19261 ctattaccgc tagccccctg gccacctaaa cgtagaccgc cgggatcgca aatatgccat
    19321 cgtgtatctg cccctgtgcc cacgttttcg aaaggaaccg cagtaggaga actgccttta
    19381 acctgcaata aaactttggc ggcaagatag tcattaattt tgtaccagtt gtcgcctaaa
    19441 tagactaacg gtgaatccgc ggcgaatgcc catcggccac ttctgacatt gtctttatcg
    19501 caatcacagg aaacataata atcattcgat tcgttccact gataaaattt gtctttaact
    19561 actccagccg tattttcttc ttcatccaga gttttaacgc caaaattgac gccaatatac
    19621 ggattcccct gagtggtatg acactgcccg atataagcag cagtttgagc gcaccatagc
    19681 attaagacgg cggcaaaaaa tagtcgtagt aataacacgc atttttctcc aaagattatc
    19741 tgcatatggc atccattcgc gtaatcgcag agttaatttg ttgttcggta agctggtaac
    19801 tgatatgaca aatttgatcg ggagcttcgc cccagcgtac agttatcgct ccctgagcgg
    19861 gcaaaccgga catatagagc gagccatcat catcaataat gccgctatta gtctgtatgc
    19921 cgtccagcgt cgctatcgcg ccaaaacgca gcggtatacc ctgctttgat gttttcatta
    19981 atacccttgc tcccgcatgg gcattgaact ccgccagaac caacgcccct ttggtgggaa
    20041 ccacgttgac tactgcattt tcaagatcca cgttacgttt taacgatgcc gcatcaagtg
    20101 cgattcgatt ttcccgataa gaagtggcat aggggacaac ggtatatccg cgccagtcag
    20161 ttttcagccc ggtgctgttc gccacacgag tattctctgc gcctggtgct gcaatcagca
    20221 cgttagtttc ccccagcgat tgccccaggg taatgccctg tgaatgggca actaaactgc
    20281 ctgaaagagc gtagttcagt tgttgttgac tgccgttatc gctgtagttg tagcccactc
    20341 gggcatctgc aaacgcccct ttgtagtcca tgctggcgct accattagcc gttttcccct
    20401 cgctgttata tccctgttgc acgctgtagc tcaggttacc gtcatcgagt agtgtctccg
    20461 ttaaacccag gttttgtgta atgtgccctt cgttatcgat actggtgtta gaggtcatcc
    20521 aggcgtaatt ttttttcctg gtatagctat cattacttct ggggagcaac agattgagcg
    20581 gcagggaaaa atttagtgca aacacctgat cagcatcagg ttgaccacgg gacttactgt
    20641 agttccagga aacccccagc gagagatctt taatttgcgt gttgtagcca aactgtaata
    20701 gccgatcctg ttgatcggta tgccagtaag tttgctggct accacttaaa taaaacaaac
    20761 cgtactcgcc taattgctgg gagatattga cctgcagttt gccacgtttg gtgtaaaaca
    20821 aattgtaata acgcgaccac atcggcgtat cttcatcatc accgtcatta aattcgtagc
    20881 catccatatg tttatacatg gtgtcggaaa gggtgtagaa acccgacgtc gaatagcgat
    20941 agcccatcaa ttgaaaatta gtgcccaatt ggttgagcgt tttggcatac agaaaacgat
    21001 atgactgacc tttatgttcg ctttggtctg ccagcgtact cttcgcctgg gtcgcatcga
    21061 acgaaattgc tccgaaatcg ccaaggttaa atcccagacc aaacatggcg gcacggtaat
    21121 attcagcata ttgtccacca ccataccatg tcgttcccca cggtccgccc cactgcaacg
    21181 tggtctgggc aaatttgctc tcctgctgtt cattactatt ggttctgtat ttcgccagtg
    21241 tcaccgcgta tttgattcgc ccctgacgct ggagtaatgg cacgctggaa aacggtacgc
    21301 tgtagctatt gacgctgccg tccgcttctt tgatttcaac taacaaatca ccgctcgacg
    21361 acgtggaata gagatcacta atttcaaaag caccaggcga tacataagtt tggtaaatgg
    21421 tgtaaccatt ttgcttaatc gttatctggg cattgctttt ggcgatacca cgcaccacag
    21481 gcgcaaaccc tttcaggctg tctggcagca tgttgtcatc tgacgccagc tgcacaccac
    21541 gaaagctgac actatcgaaa aaatcgcctg cagtataatc atcacctacg gtcagttcgc
    21601 cttttagtgg aataaccgcc cgctgcaaat agctgctgag attcttccat tctgcggttt
    21661 ggccactact gcgactccat gttgaattgt tgcgcaatct ccatgggcct aaattaacgc
    21721 cactgtttaa attcagaaaa tagctgtcgc cagaatcact gtctgcgctg ctatgaatac
    21781 tgttagcccc gctaaatagc gcgttgatcc cttcatccca acgttcaggg gggacataac
    21841 cacgggcatt acttttgatg gcgatttgcg gtactgaaat ttccagac
//
LOCUS       DAJTUN010000061        21737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTUN010000061 DAJTUN010000000
VERSION     DAJTUN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.061"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..544
                     /locus_tag="OO470_002191"
     CDS             <1..544
                     /locus_tag="OO470_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6251427.1"
                     /translation="DSGTITLGETVKLASVDQFRDSMDNSKTVWEEVSGGLDIMKIGN
                     TEMPSRAYVGRFNFKGVDQGKRVGELSGGERGRLHLAKLLQVGGNMLLLDEPTNDLDI
                     ETLRALENALLEFPGCAMVISHDRWFLDRIATHILDYQDEGKVEFFEGNFTEYEEYKK
                     RTLGADALEPKRIKYKRIAK"
     gene            complement(651..818)
                     /locus_tag="OO470_002192"
     CDS             complement(651..818)
                     /locus_tag="OO470_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251428.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(852..2084)
                     /gene="nadR"
                     /locus_tag="OO470_002193"
     CDS             complement(852..2084)
                     /gene="nadR"
                     /locus_tag="OO470_002193"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6251429.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(2105..3487)
                     /gene="radA"
                     /locus_tag="OO470_002194"
     CDS             complement(2105..3487)
                     /gene="radA"
                     /locus_tag="OO470_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6251430.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(3536..4504)
                     /gene="serB"
                     /locus_tag="OO470_002195"
     CDS             complement(3536..4504)
                     /gene="serB"
                     /locus_tag="OO470_002195"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6251431.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            4610..5254
                     /locus_tag="OO470_002196"
     CDS             4610..5254
                     /locus_tag="OO470_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6251432.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            5282..6297
                     /gene="lplA"
                     /locus_tag="OO470_002197"
                     /pseudo
     CDS             5282..6297
                     /gene="lplA"
                     /locus_tag="OO470_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            6329..6592
                     /locus_tag="OO470_002198"
     CDS             6329..6592
                     /locus_tag="OO470_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6251433.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(6753..7472)
                     /gene="deoD"
                     /locus_tag="OO470_002199"
     CDS             complement(6753..7472)
                     /gene="deoD"
                     /locus_tag="OO470_002199"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6251434.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(7529..8752)
                     /gene="deoB"
                     /locus_tag="OO470_002200"
     CDS             complement(7529..8752)
                     /gene="deoB"
                     /locus_tag="OO470_002200"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6251435.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(8804..10126)
                     /gene="deoA"
                     /locus_tag="OO470_002201"
     CDS             complement(8804..10126)
                     /gene="deoA"
                     /locus_tag="OO470_002201"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6251436.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(10253..11032)
                     /gene="deoC"
                     /locus_tag="OO470_002202"
     CDS             complement(10253..11032)
                     /gene="deoC"
                     /locus_tag="OO470_002202"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6251437.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11290..12831
                     /locus_tag="OO470_002203"
     CDS             11290..12831
                     /locus_tag="OO470_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6251438.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            12803..13666
                     /locus_tag="OO470_002204"
     CDS             12803..13666
                     /locus_tag="OO470_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6251439.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(13880..14662)
                     /locus_tag="OO470_002205"
     CDS             complement(13880..14662)
                     /locus_tag="OO470_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6251440.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(14659..15732)
                     /locus_tag="OO470_002206"
     CDS             complement(14659..15732)
                     /locus_tag="OO470_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6251441.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(15854..16015)
                     /locus_tag="OO470_002207"
     CDS             complement(15854..16015)
                     /locus_tag="OO470_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6251442.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(16132..16737)
                     /gene="osmY"
                     /locus_tag="OO470_002208"
     CDS             complement(16132..16737)
                     /gene="osmY"
                     /locus_tag="OO470_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6251443.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(17130..18719)
                     /gene="prfC"
                     /locus_tag="OO470_002209"
     CDS             complement(17130..18719)
                     /gene="prfC"
                     /locus_tag="OO470_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6251444.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(18810..19487)
                     /gene="yjjG"
                     /locus_tag="OO470_002210"
     CDS             complement(18810..19487)
                     /gene="yjjG"
                     /locus_tag="OO470_002210"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6251445.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(19502..19948)
                     /gene="rimI"
                     /locus_tag="OO470_002211"
     CDS             complement(19502..19948)
                     /gene="rimI"
                     /locus_tag="OO470_002211"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6251446.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(19917..20330)
                     /gene="holD"
                     /locus_tag="OO470_002212"
     CDS             complement(19917..20330)
                     /gene="holD"
                     /locus_tag="OO470_002212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6251447.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            20433..21464
                     /gene="rsmC"
                     /locus_tag="OO470_002213"
     CDS             20433..21464
                     /gene="rsmC"
                     /locus_tag="OO470_002213"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6251448.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         5062 a         5988 c         5625 g         5062 t
ORIGIN      
        1 ggacagcggc accatcactt tgggtgaaac ggtgaaactg gcatcggttg atcagttccg
       61 tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatattat
      121 gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg
      181 ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt gagcgcggtc gtctgcatct
      241 ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct
      301 ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgtgcgat
      361 ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggactacca
      421 ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag agtacaaaaa
      481 acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc aagtacaagc gtattgcgaa
      541 gtaatgcgta aaatgccgga tgcggcgcga acgccttatc cggcctacaa aaccagcaat
      601 ttcaatacgt tgtaaaactg taggcctgat aagacgcggt aagcgtcgca tcaggcattg
      661 agcaccgaat gccggatgcg gcgtgaacac cttttccggt ctacaacagc cttagcatcc
      721 tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac cttgtttaat ctcgacagcc
      781 tcttccagag aggttttcaa ctcatcaaaa aaactcattt tatacctcgc ctttgagcag
      841 tttcgtcacg gttatccttg ctcccccatc atctcccgca ccagctccac gcagcgcagg
      901 aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
      961 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
     1021 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
     1081 tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
     1141 gtagtgacaa aatcggtgtc aataaacgcc actttattgg cgtatttcac tgcaaaatca
     1201 atgtattgtg cgtggcccaa cgcgatttta tcgtagtcgg aatactgcaa tgcgatctca
     1261 tcaccgccga ggtgtgaaaa gacataatcg cggccatatt cccacgcact ggtggtgttg
     1321 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc gccaaggatc
     1381 gccacggtac gcacaaaaaa cggcttcact tcggtaggaa tatattccca gtagcggaac
     1441 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
     1501 accgtctcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
     1561 atcaggtccg gctggatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
     1621 tcccagccat gcggatacgg ctccatgccc tcttcgttga aagcatgaat acggatattt
     1681 ttctgatatt taaaggtttg caatagccag cgcagacgat ccggcaccgt gggctgctgc
     1741 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
     1801 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
     1861 gggtagaact taccgaatac gacaccaatg gttttcttct gccggggaaa ttcaagcccc
     1921 aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
     1981 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
     2041 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
     2101 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
     2161 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
     2221 cgtgtttcgc cgcttcagaa atccgctcct ggccactggg caccgggcgg atctcccctg
     2281 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
     2341 aaaccatcgc cagcagtaac gctaagtcgc cgctggtttc ggttaccttc acgccgccga
     2401 ccacgttcac aaacacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
     2461 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
     2521 ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
     2581 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
     2641 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
     2701 cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg ggagtcggca tcgccatcca
     2761 gaagcaccga acagtcgata cagtgttcca gcactttcgg gccagccagc gaaccatctt
     2821 tggttacgtg tccaaccatg acaatcgcca caccgcgcgt tttggcgaaa cgcgtcagat
     2881 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
     2941 gcatcacctg gatcgagtca attaccatca gcttcggttg ctcttcttcg gcaatcaggc
     3001 agatctgctc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
     3061 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc ggtgacatac agcgttttca
     3121 tctgctgggc cagtttgcac agcgtttgca gcaacagcgt ggatttcccc gcaccagggt
     3181 taccgccaat cagaatggca cttcctggca ccacgccgcc tcctagtacg cggtcgaact
     3241 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
     3301 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
     3361 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
     3421 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
     3481 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atgacgggca ggcaattact
     3541 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
     3601 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
     3661 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
     3721 tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt gtactgcgcg tctacgatgt
     3781 cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
     3841 ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa agtaaagccg ccggaggcaa
     3901 tcgccacttt ccagcccagc gtttccagct tgagcaccag ctgcgttaag cctggcatca
     3961 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
     4021 cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttacct
     4081 ccgccaccat ctcgcccgtt ccggccagct tggcaatttc atcaatacat tcaatctgga
     4141 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
     4201 ccagcggggc gacatccagc tgcgcttcgt gggccaggcg tgtagcccgt gcggtgagtg
     4261 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
     4321 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
     4381 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
     4441 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
     4501 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
     4561 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
     4621 aaaactgaaa ttccggctac atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
     4681 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
     4741 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
     4801 gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt taacagatga
     4861 aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cacgttctgg
     4921 cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt aaaaaagcag gcggctattt
     4981 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
     5041 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
     5101 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
     5161 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
     5221 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
     5281 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
     5341 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
     5401 tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
     5461 gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc ggtggcggcg cggtgttcca
     5521 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaactct
     5581 ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
     5641 acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc aaagtctcag gctcggccta
     5701 tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg ctactcaatg ccgacctcag
     5761 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacttc
     5821 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggctcacca tgagcaggtt
     5881 tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcacgtgga agcggaaatc
     5941 atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgtcagagt
     6001 agctgagagt ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt
     6061 agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag
     6121 gtatttaccg acagcctcaa cctcgcgccg ctggaagccc tcgccgggcg actgcaaggc
     6181 tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg
     6241 gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc
     6301 gctgattaaa atatccaagg aaggaaatat gatcgatctg gaaaaccagg aacgggagat
     6361 tatcaattta atgttcagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa
     6421 attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaacagat
     6481 cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atggcagaaa tttatggttg
     6541 tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagattc
     6601 gtgaatagat acttgaatgt tttttatttt ctatccatat gaaaaataat agtaaatcta
     6661 taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc cactccggct
     6721 taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag cagaacggat
     6781 tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc agtggtctgc
     6841 tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc gccaaactct
     6901 gcagccacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc gtatttttcc
     6961 atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa caggttaccc
     7021 acgcgagcat caacgcccag tgctttagct gcatctactg cgttacgcac catgtcaaag
     7081 tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt ggaatcggtg
     7141 caggccccca taccgataac gacgtcgcgc agttttacgt gcggcagaac tgcgccacag
     7201 gaaccaacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt ggtgtagatg
     7261 gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc tttgtaagta
     7321 ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt
     7381 tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc agcgaaatcg
     7441 cccatttctg cattaatgtg tggggtagcc attgttttat cctttttgtg acataacaaa
     7501 ggcggagtca acgctccgcc caaatccatc agaacatggc tttgccatat tccatatcag
     7561 aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca cggtgaccca
     7621 gtgagcccgg ttttactttc gggccatata ccagtaccgg aatgtgttca cgcgtgtggt
     7681 cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt gaggatcagg atgtcgtcat
     7741 cgcgcagcag agacatcagc tccggcagac ggcggtcgaa cagttccaga cccgcagcat
     7801 aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga
     7861 cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca
     7921 agccagtcgc tttcactttt ttggtgatac cgcagttggc gtagatgtct gcaattttac
     7981 cgacagaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg gtcggtgctg
     8041 gcggctcaac agccaagtcg tgacggttac cggtacgctg gaagttacca gctttgtcac
     8101 cgataaacgg acgagcgata acacgaccga tattgtagcc gccgttggtc agctcttcac
     8161 gggcgatttc gcacagttcg tagagtttat ccagaccgaa agtttcttca tggcaggcaa
     8221 tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc atgtgctctt
     8281 cgcccagttg atccagaatg accgtaccgg aagagtggca gttaccgagg taacccggca
     8341 gattagcgcg ttcgaccagt ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg
     8401 agaaatatcc ccactcaaac agaaccggga caccggcgat ttcccagtga ccagacgggg
     8461 tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc
     8521 cgtccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc agccccagac
     8581 gggtcagatt tggcagattg agcggccctt tacgaccatt atcagcttcg cctttggcac
     8641 aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt tctgcatctt
     8701 ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc atatgttctc
     8761 cgtactcagt gcttcaaatt taaatgatca gatcagtata ccgttattcg ctgatacggc
     8821 gatagacagt tggtgtgctt tccggtgcgt tatcggcaag tttaattgcc gctttcaccg
     8881 ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag gataaccgcc agcggacgct
     8941 gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa gccgacgctg taatcgatgg
     9001 tgtcagatgc ctgacgacga ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg
     9061 tatccatttc actgacaaaa ccttcggtat cagcatagac tgctttcgtc agcatcgctg
     9121 tcggcagata cttcgcgtag ttctcaacga agtcggtcgg gcctttttgt gccgctacca
     9181 tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg
     9241 cttcggcgtc atctttcgcc agtttgccgg agatcagcat ctccacgcac agcgccatcg
     9301 tgacatcaaa cagacgcggg ttacgatact cacccgtcag gaactgcacc gcttcacgaa
     9361 cttcaaccgc gttacctgca ctggaggcca gtacctgatt catgtcggtg agcagcgcgg
     9421 tggtgcgcac gccagcgccg ttagccacgc caacaatcgc ttcggcaagg gcttcagaga
     9481 gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc agcgcgtcca
     9541 gaccttccgc cagtttcttc gccaggatcg aggcagtgat cagcgggatg gagtccacgg
     9601 ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc cggagccagt gaactggtct
     9661 gaccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg tcatccggga
     9721 aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta tgaccgaggc
     9781 cgcgaccgga gatcatcgga atatagccgc cgcaggctgc gaccatcggc cccaacatca
     9841 gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc aacaatcggg ccattcagat
     9901 gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc agcgagacac
     9961 gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca atctgccctt
    10021 cggaaatagt gttgtcgcga ataccgttga taaagaaacg aatttcttca tcgctcagcg
    10081 catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta acctcccgag
    10141 gaaaagagta tgaggcggct tttgccggat gcgtctacgc cttatccggc ctacgggtag
    10201 atacgtctcg taggcctgat aagcgcagcg catcaggcgt aaagcatctt acttagtagc
    10261 tgctggcgct cttaccgtcg ccgtggccca gcgctttcag caggcttgcc agcaggctgg
    10321 aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc accgaacagt tcatctgcga
    10381 tggcgagata tttctgcgca tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa
    10441 cggttttttc tacgcccata tcacggatca cttccatcat gatgcgagcg ctttccggcg
    10501 tcgcgttcac agccacttta ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg
    10561 agatttcaga cgctttacgg atcagcgctt cgtctttcag ttcgccggtt tcgatgatca
    10621 ctttcagcag tacattcgct gccgcgcaag cctctttaca ggctttcacc aggtcaaaac
    10681 caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa caccacgtca acttcatcag
    10741 caccgtaggc gattgccgca cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt
    10801 gtgggaagtt ggttaccgta gcgatacgga tttccggggt gccctgctct ttcagagttt
    10861 tgcgagcaat cgggataaaa cgaggataga tacagatagc ggcggtattg ccgaccggag
    10921 ttttggcctg atgacacaga gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg
    10981 tcaggtccat caatttcagt gcacgcaggc tgcttgcttt cagatcagtc atttcattct
    11041 cctggcttgt cgccaataaa attcaccttg cgagtttgtt agtattctaa catctactcc
    11101 gcaacacact tcgatacaca tcacaattaa ggaaatctac ttacaagttt gcatcactgt
    11161 aatgcgatct ggttcaaata attcactttc aaatgaatgc gtcagtggtg gcaaacgcat
    11221 caggatcaaa gtgaacatca cgaaacttct tacaatggcg catcaccttc ggcataaaag
    11281 gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc
    11341 accagcccag tgcttagccc ggagcagaag cgccattttc tggcactgga agcagaaaac
    11401 aatctgcctt acccacagct tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc
    11461 tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct atgtcttacc cgattacgct
    11521 cgttttctgg cgaacggttc cgaatggctg gagctggaag gcgcgaaaga tcttgatgac
    11581 gcactctctc tgctgaccat tctttaccac cacgtaccgt cggtcacatc gatgccggtc
    11641 tacctggggc aactggatgc gttgttgcaa ccgtatgtta gaattctaac acaaaacgag
    11701 atcgatgttc gaataaaacg tttctggcgt tacctcgaca gaaccctgcc agatgccttt
    11761 atgcacgcca atatcggccc gtctgattcg cccattaccc gcgcgatctt acgtgcagac
    11821 gcgcaggttt caccgaacct gacctttatc tacgatcctg aaatcacccc tgatgacctg
    11881 ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac cgcacatcgc caacggtccg
    11941 gtgcatgata aaattttcac aaaagggggc tacgggattg tgagctgtta caactcactg
    12001 ccgctggcgg gtggtggcag cacgctggta cgccttaacc tgaaagccat tgccgagcgc
    12061 agcgaatcgc tggatgactt ctttacgcgc actctaccgc actactgcca gcagcagatc
    12121 gccatcatcg atgcgcggtg tgaattcctc tatcaacaat cacacttctt tgagaatagc
    12181 ttcctggtga aagaagggct gattaaccct gaacgttttg tgccaatgtt tggcatgtat
    12241 gggctggcgg aagcggttaa cttgctgtgt gagaaagaag ggattgccgc gcgctacggt
    12301 aaagaagccg ccgcaaatga agtgggttat cgcatcagcg cgcaactggc ggagtttgtc
    12361 gccaataccc ccgtgaaata tggctggcaa aaacgcgcca tgttacacgc acagtcgggg
    12421 atcagttccg atatcggcac cacgccgggc gcgcgtttgc cgtatggcga tgagccagat
    12481 ccgatcaccc atctgcaaac tgtcgccccg catcatgctt attattattc cggcatcagc
    12541 gacattctga cgctcgacga aaccatcaaa cgtaacccgc aggcactggt acagctttgc
    12601 ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg
    12661 gttcgcgtta ccggttatat ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt
    12721 tcacgcacca acaccacctg gctgggcgaa gaagccgcac gcaacactcg tattctggaa
    12781 cgccagccgc gcgtgataag ccatgaacag cagatgcgct ttagtcagta agattatccc
    12841 cttctcctgc gttgacgggc caggcagtcg tctggctctg tttttgcagg gctgcaatct
    12901 gcgctgcaaa aactgtcaca atccgtggac gatgggacgt tgcaatgact gtggggagtg
    12961 cgtgccacag tgtccgcatc aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc
    13021 tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt ccgcaacatg ccacaccaat
    13081 ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg cgcaaagcgg tgctgtttat
    13141 tgaagggata acggtaagcg gcggcgaagc cacgacccaa ctgccgtttg tagtggcgct
    13201 gtttactgct atcaaaaacg atccgcaact gcgccatctc acctgtctgg tggacagtaa
    13261 cggtatgttg agcgaaaccg gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct
    13321 cgatctcaaa gcatggggta gcgaatgtca tcaacaatta accggacgcg ataatcaaca
    13381 gattaagcgc agcatctatt tgctggcaga gcgcggcaag ctggcggaac tgcgtttgct
    13441 gatgatccca ggccaggtgg attatttgca acacatcgaa gaactggcgg cgtttatcaa
    13501 gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat gcccacggcg tgtatggcga
    13561 ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag ccgttggctg atgcattaaa
    13621 ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat ttgtgacatg ccggatacgg
    13681 cctacggttc ggcacagact tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat
    13741 tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg gcctacggtt cggcacaaac
    13801 ctgtaggcat gataagacgc gttaagcgtc gcatcaggca ttatgcgccg actgccggat
    13861 gctgcctacg aagggcaaac tacccacgaa cgttaaacac cgcatacgtg ttattaagca
    13921 acacttcggc aatctcatcc gcgggttccg ggcgtaactc gcaaagcacg gcaaacacac
    13981 gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc gttgagcggc atatccggcg
    14041 catcggtttc cagcaacaac gatgccagcg gtaattttgc gatgacatcg cgggttttac
    14101 tggcgcgtgg ataggtgata gtaccgccta cgccgatttt gtagcccagc tgtacaaacc
    14161 gttcagcctg ttgctggctg ccagaaaatc cgtggacaac accagcacgc ggcaaatcgt
    14221 ggtgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg tgaatgcagg atcaccggca
    14281 gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa ccactgctgc ctctcaaatt
    14341 gcggatcgtc gccgaagaga tccagaccga tctcccccac cgccaccacc ttcgccggac
    14401 gcctttccag cgcctgctgt agctgctcaa gagacacatc gctatgtttt tccaacatac
    14461 caggatgcaa gcccaatgcg gcatacagcg gttgataatt ttccgctaac gcctgcacgc
    14521 gggcaaaatt ttccgcctct gtcgccggaa caatgatctt gcctacgccc gcttgtgccg
    14581 cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg gaaatcaaaa tggcagtgag
    14641 tgtcgataaa acggcaaatc aagccagatc ctcattgtta aatgtcgggt cgttcgcctg
    14701 cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg actggcgcag gcggaatgac
    14761 aatcgattcc ggcgtcacca ctggcaccag atggcgggta agcggcgctt tttcagttaa
    14821 tagcttgcca accgtggcga ggaaataacg accgcataaa cgcccaagtt tatagtcttc
    14881 acgcagcgcc ggaatccgac tgccaagcgc gatactatgt aatggcttcg gcggataaat
    14941 ttcggatatc cgcagcttgc ccggtggttt ctcaataaat tgctgaatgt cacgatagct
    15001 ggtttcatga tgctgcacca gattgaccag cggctgcagg ctactgtcac ccagccagcg
    15061 ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt gacggcacag tgcggatgac
    15121 gaccaacgtt ttagcgccct gccttgccgc ctctttaacc ggaatcgcat cactgatccc
    15181 gccatccagg tagttaatgc cttccagcga cactccgcta cgataaaagc caggtatcgc
    15241 actggaggcg cgaatcacat ccagccagtt ttgtttggtt ggtaaaaagt aattcggctc
    15301 gtagtcatcc tgacgacagg cgcacatata aaacgatttg ccgctgtcaa acaaccgcgc
    15361 ggcggtatcc atttgcaacg gcatctggct tgcggtggcc tccaccagcc agtcgagatc
    15421 gataagattt cctccacgga caaagcgcaa tggatcgaaa aattcgcgtt ttgtggtata
    15481 gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag ataaacgccg agaggttctg
    15541 cgccccggca gatgtgccga gataaagatc gaaaggatta aactgcgcgc gcataaactc
    15601 atccagcacg ccagccgtga aaattccacg ctgtccgccg ccttcgcaca ccagagctat
    15661 tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata ttaccaagcg ttacaggtat
    15721 tcgctgcccc acttctgcct tcctgttttt gttgtgaaag aaagacaaag taacgcaatt
    15781 tattattcgc taaaaccgca aaagccagtc cgctggactg gctttggata aaattttctt
    15841 aatggtctga aatctagggt cgttttcggc ccatgaacaa actcactagg aacagaataa
    15901 tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt accggccaga ccaccaaacc
    15961 caagtgcggc ggcgattaac gcgataacca gaaatatgat gccccaacga aacataagcg
    16021 tctcctttac catagttaat gtcaccgctt gatatgagcg tgaaaatcac tcaatgcgat
    16081 atttttagtg tggtgcacat cgcgcctccc gacaaagttc gggaggacga attacttagt
    16141 tttcagatca tttttaacgc ttttcacacc atctaccgct ttggcgatac tttcagcacg
    16201 gtcactttgt gcctgagaat ctacggtacc ggagagctga accacgccat cggtggtttc
    16261 aactttcact ttacgtgaag ggacgatatc gtccgccagc agtttggctt tgatttcact
    16321 ggtggtggcg gtgtcacccg cgtagccctt caccgagcct tctttagcgt cgcgaacgtg
    16381 cagtttgtcg ctgacagagg tcacgccttc aacgcctttc gccactttca ctgcctcttc
    16441 ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg actttttgat cggttttgac
    16501 agagatatcg gtgctcttga tgttgtcatg atccaccagc gccgccttca ctttcgcggt
    16561 gatggcgctg tcatccatga aattaccgac tttattcata gagctatcga ctttttgccc
    16621 tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc
    16681 agaggtcaac attacagcca gcagagtttt cgaaatcttc agtcttgtca tagtcatcga
    16741 tttattcctg tatgtttgct cgtaatttga gcctagcaac acgaggttgc attgctgaat
    16801 agggagagac ttcaccctct acagaagtca atggtcgcca tcacaaaagc gatgggtgat
    16861 gaataacgac cattacagcc tctgaatcag ttattaatat cggtagaatg acaatcgacg
    16921 gctttagata ctgatatcta cgcattgaac ggtatttaac gccatcagaa atgtcatcac
    16981 tttgttaaat atagatcaca attttgaaac cgctcgggat atcagcgaaa acataagcaa
    17041 aagtgaatgt tttaagaaca ttccgtaagc ggctaataag gaagggaaat tgacagggcg
    17101 cagcggctac cgcgccctgg aggcaagaat taatgctcgc gggtctggtg gaactgaacg
    17161 tccggataac gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg
    17221 ttatcgccgc catcaagcgc cagttggctt tcgttcttac gcttgaactc ttcgaatttc
    17281 ttcgcgtctg cacattctac ccagcgggca gtggcaacgt tgactgactc atacactgct
    17341 tcaacgttgt attcgctctt caggcgcgct accaccacat caaactgcag cacaccaacc
    17401 gcaccaacga tcagatcgtt gttggagatt ggacggaaca cctgcaccgc gccctcttcg
    17461 gaaagctgta ccagcccttt gagcagctgt ttttgcttca gcggatcttt caggcggata
    17521 cgacggaaca gttctggtgc gaagttcgga ataccggtga acttcatcat ctcaccctgg
    17581 gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca ggccgaggat atcgcccgga
    17641 tacgcttctt caacgtgcga acggtcaccc gccataaagg tcagcgcgtc ggagatcacc
    17701 acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt accggacacc
    17761 acacgcataa acgccacgcg gtcgcggtgt ttcggatcca tgttggcctg aattttaaat
    17821 acgaagccgg taaatttatc ttcgctcgct tctacggtac gggtatcggt ctgacgcggc
    17881 atcggcgcag gcgcccactc caccaggcca ttcagcatat gatcaacgcc gaagttaccc
    17941 agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac
    18001 tcgttagacg cgcctttaac cagttccagt tcgtcacgca gctgctgtgc cagatcttca
    18061 ccaaccgcag catcgagatc cgggttattc agccctttaa caatgcggac ttcctgaatg
    18121 gtgtgccctt taccgctctg atagagatag gtttcatctt tataaaggtg gtaaacgcct
    18181 ttaaacagct tgccgcagcc aattggccaa gtgatcggtg cgcagccaat tttcagctcg
    18241 ttctcaactt catcgagcag ttccatcggg tcgcggatat cacggtcaag tttgttcata
    18301 aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg ggtacgatct
    18361 tcaacacctt ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt cagggtacga
    18421 taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg
    18481 tgatacggaa actgcatcac agacgtagta atggagatcc cacgctgctt ttccatctcc
    18541 atccagtccg acttagcgtg ctggttggaa ccacggcctt ttactgtacc ggcggtctga
    18601 atggcctgtc cgaacagcag caccttctcg gtgatggtag ttttaccggc gtccgggtga
    18661 gaaataatgg caaaagtgcg gcgcttcgcc acctctttca aataaggaga caacgtcata
    18721 attctttctt cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc
    18781 tattttaccc atcaacgggg gggaggcaat cagtgtttac acaggagctg ctccagttcg
    18841 tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca
    18901 ttcagccagc aggtcgcaag cccggcgttg atgccaccga gaatatcgga ctcggcagtg
    18961 tcgccaacca tcagcacgcg tgaacggtca ggattgcccg cctgttccag cgcataatcg
    19021 aaaattttct tattcggttt ggcaacgcca acttcttcgg aaatcaccag caaatcgaag
    19081 taatcacgca ggcccgtccg ttccagacgc acttgctgca aggcgctaaa accgttggtg
    19141 atgatgccga ttttagcgtt gctacgaatg gcgttaagca gagaaaccgc gcccggcaac
    19201 ggcgtacaga tttccgccat cgcattaata aaggcatcgt taagcaagcc tgcttcaact
    19261 ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg
    19321 ccgttttgat aatccaccca cagtggcttg ttaacggcct gatagtcctg aaaatcttca
    19381 gcggtaaagg tgacgctgta atcaagaaac atccgctgca ggccggtgaa tgagtcaaag
    19441 gtaaacagcg tttcatcggc atcaaagaaa atccagtccc acttcattat tccaccttgt
    19501 cttacatact gattggcaac gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt
    19561 agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg
    19621 cagcatttga ggcacggact tccagccata gtgtcgccac gccgcgtttt tccagttcgt
    19681 cgatcagatg ttccagcagc gccctaccca gcccctgacg ctgatagtcg ggatcgaccg
    19741 caatattgaa caatgtagct tcatccagca ccacttgcgt aatcgcaaac gccgccattt
    19801 tgccgttttg cgttaactga aagttgagat aacgctcgcc ctggttgctg gcaaaggttt
    19861 tttcactcca cggaaaggag tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag
    19921 tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt
    19981 gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc gccttccaat
    20041 gatagcggtt cgtcagtacc caaccgccag ctgttgcagc gactgccttg cggcagcatc
    20101 gcgatttttt ctggcgtcag ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga
    20161 acatcgctca ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat caccagacgg
    20221 acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac
    20281 cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc
    20341 tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac atatggctat
    20401 aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc atttaccccg gcaagtgaag
    20461 tcttgctgcg tcacagtgat gatttcgaac aaagccgtat tctgtttgcc ggagacttac
    20521 aggatgacct gcccgcgcgt ttagataccg cggccagccg tgctcatacc cagcaattcc
    20581 accattggca ggtgttaagc tgccagatgg gggataacgc ccgttttagt ctggtcgcca
    20641 cggcgaatga tgtcgcagat tgcgatacgc tgatttacta ctggccgaag aacaaaccgg
    20701 aagcccagtt ccagttgatg aatttacttt ctctgctgcc agtggggaca gatatttttg
    20761 tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca gattatgcgc
    20821 cgttgaataa agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac
    20881 agccagtatt tgatgcggat aaattctggg gcgaatacag cgtcgatggc ctgacggtca
    20941 aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt cggtagccag ttgctgctct
    21001 cgacgttaac tccgcacacg aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac
    21061 tttcagttgc ctttgcgcgc cattcgccga aaattcgtct caccttgtgc gatgtctctg
    21121 ccccggcggt agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct
    21181 ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga tatgatcatc tccaacccgc
    21241 cgttccacga tgggatgcaa accagcctgg atgcggcgca aacgctgatt cgtggcgcgg
    21301 tgcgtcatct taatagcggc ggcgagctgc gaattgtagc gaacgccttc ctgccttacc
    21361 cggacgtgct ggatgagaca tttggcttcc atgaagttat cgcgcaaacc gggcgcttca
    21421 aggtatatcg cgccattatg acccgccagg cgaagaaagg ttgagtatct gatatctgtc
    21481 gccgcatacc gtgtcggatg cggcgtgaac gccttatccg acctacaaaa cctgcacgtt
    21541 agcccttcgt aggccagata agacgcgcca gcgtcgcatc tggcatctgc ccccgcatgc
    21601 ctaccaaacc tgcacgctag tttcctgatg gacatttttc cagcaattac acatcaggcg
    21661 ataattaact attgacgaaa agctgaaaac cactagaatg cgcctccgtg gtagcaattc
    21721 tttttaagaa ttgatgg
//
LOCUS       DAJTUN010000062        21671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTUN010000062 DAJTUN010000000
VERSION     DAJTUN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.062"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(354..617)
                     /gene="cedA"
                     /locus_tag="OO470_002214"
     CDS             complement(354..617)
                     /gene="cedA"
                     /locus_tag="OO470_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6251449.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            800..3061
                     /gene="katE"
                     /locus_tag="OO470_002215"
     CDS             800..3061
                     /gene="katE"
                     /locus_tag="OO470_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6251450.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(3108..3866)
                     /gene="chbG"
                     /locus_tag="OO470_002216"
     CDS             complement(3108..3866)
                     /gene="chbG"
                     /locus_tag="OO470_002216"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6251451.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3879..5231)
                     /gene="celF"
                     /locus_tag="OO470_002217"
                     /gene_synonym="chbF"
     CDS             complement(3879..5231)
                     /gene="celF"
                     /locus_tag="OO470_002217"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6251452.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5336..6178)
                     /gene="chbR"
                     /locus_tag="OO470_002218"
                     /pseudo
     CDS             complement(5336..6178)
                     /gene="chbR"
                     /locus_tag="OO470_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(6186..6536)
                     /gene="chbA"
                     /locus_tag="OO470_002219"
     CDS             complement(6186..6536)
                     /gene="chbA"
                     /locus_tag="OO470_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6251453.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6595..7944)
                     /gene="chbC"
                     /locus_tag="OO470_002220"
     CDS             complement(6595..7944)
                     /gene="chbC"
                     /locus_tag="OO470_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR6251454.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(8029..8349)
                     /gene="chbB"
                     /locus_tag="OO470_002221"
     CDS             complement(8029..8349)
                     /gene="chbB"
                     /locus_tag="OO470_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6251455.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8648..8986)
                     /gene="osmE"
                     /locus_tag="OO470_002222"
     CDS             complement(8648..8986)
                     /gene="osmE"
                     /locus_tag="OO470_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6251456.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9188..10015
                     /gene="nadE"
                     /locus_tag="OO470_002223"
     CDS             9188..10015
                     /gene="nadE"
                     /locus_tag="OO470_002223"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6251457.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            10245..11132
                     /gene="cho"
                     /locus_tag="OO470_002224"
     CDS             10245..11132
                     /gene="cho"
                     /locus_tag="OO470_002224"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6251458.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11092..11667)
                     /gene="ves"
                     /locus_tag="OO470_002225"
     CDS             complement(11092..11667)
                     /gene="ves"
                     /locus_tag="OO470_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6251459.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11870..12355)
                     /gene="spy"
                     /locus_tag="OO470_002226"
     CDS             complement(11870..12355)
                     /gene="spy"
                     /locus_tag="OO470_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6251460.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12685..13653)
                     /gene="astE"
                     /locus_tag="OO470_002227"
     CDS             complement(12685..13653)
                     /gene="astE"
                     /locus_tag="OO470_002227"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6251461.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13646..14979)
                     /gene="astB"
                     /locus_tag="OO470_002228"
                     /pseudo
     CDS             complement(13646..14979)
                     /gene="astB"
                     /locus_tag="OO470_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(14976..16454)
                     /gene="astD"
                     /locus_tag="OO470_002229"
     CDS             complement(14976..16454)
                     /gene="astD"
                     /locus_tag="OO470_002229"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6251462.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16451..17485)
                     /gene="astA"
                     /locus_tag="OO470_002230"
     CDS             complement(16451..17485)
                     /gene="astA"
                     /locus_tag="OO470_002230"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6251463.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17482..18702)
                     /gene="astC"
                     /locus_tag="OO470_002231"
                     /pseudo
     CDS             complement(17482..18702)
                     /gene="astC"
                     /locus_tag="OO470_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            19148..19954
                     /gene="xthA"
                     /locus_tag="OO470_002232"
     CDS             19148..19954
                     /gene="xthA"
                     /locus_tag="OO470_002232"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6251464.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20121..20829
                     /locus_tag="OO470_002233"
                     /pseudo
     CDS             20121..20829
                     /locus_tag="OO470_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            20834..21511
                     /gene="ydjY"
                     /locus_tag="OO470_002234"
     CDS             20834..21511
                     /gene="ydjY"
                     /locus_tag="OO470_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6251465.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21526..>21671
                     /locus_tag="OO470_002235"
     CDS             21526..>21671
                     /locus_tag="OO470_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6251466.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVD"
BASE COUNT         5572 a         5745 c         5087 g         5267 t
ORIGIN      
        1 tcgaaagcca ggcttttaat atttaaatca ccataattac tctgtattaa gtttgtagaa
       61 aacatctccc gcctcatatt gttaacaaaa ttattatctt atttaaatct aagtcattta
      121 caatataagt ttaagagcga cgccacagga tgaactatca aaaataactc atcatgatta
      181 gcaaaactta accattttaa aataaataaa taattaaaga aaaaagatca cttatttata
      241 gcaatagctc gtcaaaggca gctttttgtt acaggtggtt tgaatgaatg tagcaacgaa
      301 atgcagaatt tcaggtcctg taactcccgg caaaaccggg aggtatgtaa tccttactca
      361 gtcacttccc cttcctggcg gatctgattt gcccaacgtt gggcagattc aggcacagta
      421 aacgccggtg agcgcagaaa tgactctccc atcagtacaa acgcaacata tttgccacgc
      481 agcatccaga catcacgaaa cgaatccatc tttatcgcat gttctggcgg cgcgggttcc
      541 gtgcgtggga catagctaat aatctggcgg ttttgctggc ggagcggttt cttcattact
      601 ggcttcacta aacgcatatt aaaaattaga aaaactgtag tttagccgat ttagcccctg
      661 tacgtcccgc tttgcgtgta tttcataaca ccgtttccag aatagtctcc gaagcgggat
      721 ctggctggtg gtctatagtt agagagtttt ttgaccaaaa cagcggccct ttcagtaata
      781 aattaaggag acgagttcaa tgtcgcaaca taacgaaaag aacccacatc agcaccagtc
      841 accactacac gattccagcg aagcgaaacc ggggatggac tcactggcac ctgaggacgg
      901 atctcatcgt ccagcggctg aaccaacacc gccaggtgca caacctaccg cccctgggag
      961 cctgaaagcc cctgatacac gtaacgaaaa acttaattct ctggaagacg tacgcaaagg
     1021 cagtgaaaat tatgcgctga ccactaatca gggcgtgcgc atcgccgatg atcaaaactc
     1081 actgcgtgcc ggtaaccgtg gtccaacgct gctggaagat tttattctgc gcgagaaaat
     1141 cacccacttt gaccatgagc gcatcccgga acgtattgtt catgcacgcg gctcagcagc
     1201 tcacggttat ttccagccat ataaaagctt aagcgatatc accaaagcgg atttcctctc
     1261 agatccgaac aaaatcaccc cagtatttgt taggttctct accgttcagg gtggagctgg
     1321 ctctgccgat accgtgcgtg atatccgtgg ttttgccacc aagttctata ctgaagaggg
     1381 tatttttgac ctcgttggca ataacacgcc aatcttcttt atccaggatg cgcataaatt
     1441 ccccgatttt gttcatgcgg taaaaccaga accgcactgg gcaattccac aagggcaaag
     1501 tgcccacgat actttctggg attatgtttc tctgcaacct gaaactctgc acaacgtgat
     1561 gtgggcgatg tcggatcgcg gcatcccccg cagttaccgc accatggaag gcttcggtat
     1621 tcacaccttc cgcctgatta atgccgaagg gaaggcaacg tttgtacgtt tccactggaa
     1681 accactggca ggtaaagcct cactcgtttg ggatgaagca caaaaactca ccggacgtga
     1741 cccggacttc caccgccgcg agttgtggga agccattgaa gcaggcgatt ttccggaata
     1801 cgaactgggc ttccagttga ttcctgaaga agatgaattc aagttcgact tcgatcttct
     1861 cgatccaacc aaactgatcc cggaagaact ggtgcccgtt cagcgtgtcg gcaaaatggt
     1921 gctcaatcgc aatccggata acttctttgc cgaaaacgaa caagcagcat tccatccagg
     1981 tcatattgtt cctggtctgg acttcaccaa cgatcccctg ttgcagggac gtttgttctc
     2041 ctataccgat acacaaatca gtcgtcttgg tgggccgaat ttccatgaga ttccgattaa
     2101 ccgcccgacc tgcccttacc ataatttcca gcgtgacggc atgcatcgta tggggatcga
     2161 cactaacccg gcgaattatg aaccgaactc gatcaacgat aactggccgc gcgaaacacc
     2221 accggggccg aaacgcggcg gttttgaatc ataccaagag cgcgtggaag gcaataaagt
     2281 tcgcgagcgc agcccatcgt ttggcgaata ttattcccat ccgcgtctgt tctggctaag
     2341 tcagacgcca ttcgagcagc gccatattgt cgatggtttc agttttgagt taagcaaagt
     2401 cgttcgtccg tatattcgtg agcgcgttgt tgaccagctg gcgcatattg atctcactct
     2461 ggcccaggcg gtggcgaaaa atctcggtat cgaactgact gacgaccagc tgaatatcac
     2521 cccacctccg gacgtcaacg gtctgaaaaa ggatccatcc ttaagtttgt acgccattcc
     2581 tgacggtgat gtgaaaggtc gcgtggtagc gattttactt aatgatgaag tgagatcggc
     2641 agaccttctg gccattctca aggccatgaa ggccaaaggc gttcatgcca aactgctcta
     2701 ctcccgaatg ggtgaagtga ctgcggatga cggtacggtg ttgcctatag ccgctacctt
     2761 tgccggtgca ccttcgctga cggtcgatgc ggtcattgtc ccttgcggca atatcgcgga
     2821 tatcgctgac aacggcgatg ccaactacta tctgatggaa gcctacaaac accttaaacc
     2881 gattgcgctg gcgggagacg cgcgcaagtt taaagcaaca atcaaggtag ctgaccaggg
     2941 tgaagaaggg attgtggaag ctgacagcac cgacggtagt tttatggatg aactgttaac
     3001 gctgatggca gaacaccgcg tgtggtcacg cattcctaag attgacaaaa ttccggcgta
     3061 aatatggata aggtcacgtc ccctggggag tgaccttacc accttactta cacatcaaga
     3121 taactaccca accgataacc acgctcagca attgcatatt tcaacgacgc tgatgtcagc
     3181 acttccagct ccgttaaccg tgggaaacag taagcactct gacggatagt attatcgata
     3241 aacgccggat gacacatcac ctccagcgaa ggatcacccc gatggcttgc gtcatccagc
     3301 acttgcagga acagtgcctc gctaatctct tcaccataaa acgcgctact gaatccctgc
     3361 gaactgcgca gattaaccgg taaatcacca gcgttagata gcggctgacg gtcgatgcgc
     3421 aacgcaatcc cctcttcagc cgcaaacctg gcaacaatcg ggaaaatctg cgggaacata
     3481 tgcacatgat gatggctatc aagatgcgta ggtttgcgtc caaatagctc aatgaaacgc
     3541 aaatactgac tggcaagctc ctgagtaatt tcttccagcg gtaaagcatc ttcttctgcc
     3601 aactgccaga tccattttcc cagcacacca tcgcgggtta accccggcat agctgtcagt
     3661 ggcttgccca tagtaaggac aaagtgcatc cctatggcca gactcggttc atcacgactc
     3721 aactgcaccg catggtcaat agcctgccca ttcaccagcg ccgtcgtcga tgtgacaatc
     3781 ccattgcgac aggcctcgat aatgccgtag ttctgacctt tgcttaagcc aaaatcatcg
     3841 gcattaacaa tcagtaagcg ttccataatc agcctcggtt aatgtgcttt tttaagctct
     3901 gcgatgcagt cggcaaaatt tggcagccat ttctcgtgcg ccagaatcat ctcgcgtgcc
     3961 agcagctcag catcgcgatc ggaatgcacc aacggactaa ggtttagcgc cagtaacaca
     4021 tcgttaaatt ctccgctaag tgcggcgttg ctggcagcaa tctcgaagcc tttaatggtg
     4081 tgaatcagcc ccatcacttt atcatcgaaa tgcgtaatgc gcggatgtgg cgtcgcgcca
     4141 tcgtgcccca gcttacaggt catttctacc gcccagtctg ccggaatatt atcaatatgc
     4201 ccatgatgcg ggatattaac gtaatgttca gcttgcttgt cgttgtagat agcgttgatc
     4261 acttcgcacg ctgcatcaga gtaataagcc ccaccgcgct gttccagttc tttcggctta
     4321 actttcaact ccgggttttt atacagctca aaaagttgtt tctcgacttt ctgtactacc
     4381 tgcgctcgtg cgccgccttt gtagtattcg cccatttcaa tagccagcat ctctttttgc
     4441 ttgaagtaat aaagcaaata agaacacggc aacagattca gagaacgaat taagccttcg
     4501 ctaaatggca gatcgaaaat atttttaacg ccagatactt ttaactgccc ggatgccaca
     4561 ccatcaagca attcggcaaa gcgcgacttg ccatttacca gcacatcctt aatgaacacc
     4621 atatggttga ggccaaacaa atcgatagat aaatcatcgc tgtctttcag catcagaaca
     4681 tcgtgaataa acatcttcat gccgatcgga atattacaca cgccgataaa acgtttaaat
     4741 ccggtatgac gataaacggc ttcagtaacc attcccgccg ggttagtgaa gttaatcacc
     4801 catgcattcg gacaaagttc ttcgacatct tttacgatgt caaaaatcac cggaatggta
     4861 cgcagacctt taaacaaacc gcccgcgccg ttggtttcct gaccaagata accatgactt
     4921 aatggaatac gttcatccag ttcacgcgcc ggtaattggc caacgcgcag ttgggtagta
     4981 acgaaatcag catctttcaa tgcttcgcgg caatccagcg ttttataaag cttcatcggg
     5041 acgccagcgt tatcaatcat ccgttggcag agatcgaaaa taatatccag tttagctttg
     5101 ccaccctcga catccaccag ccataattcg ctgaccggca attcgtgata acgcttaata
     5161 aatccttcca gtaactccgg ggtatagctg ctcccgccac caatagtgac gacttttaat
     5221 ttctggctca taatttctcc cttcagtaca gaatactgat atctggcata tctgcccccc
     5281 cggacataaa taatccagca acaggacaga tatgtgaatt gtcaggtata acgacttact
     5341 gattaaattc agtcaatttc ttacgatatc tcttaggcgt aaaggacgtt aatttcttaa
     5401 acgttttaat aaacaaactc gggctactat aaccggcctc aaacgcaata tccgtcacgg
     5461 aatagttggt catttccagt tgtttttttg caaaattaat acggatctca ttaataatct
     5521 gcattggtgt tttgccataa tatcgttgag tcgctcgcgt caaatattcc tgtgatttgg
     5581 ctgacaacgt caccatattc tccagcgccg attcactaaa ctgctcttta tcatgcatct
     5641 tttctaccgt acttttcagc cactacggta catcatcaat cacctgttct tcgcggtaat
     5701 gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac aaactcttcc agccctgttt
     5761 cgcggaaatt taatgaagaa atcactgttt ccacataggt gagaaaagcg ttattggtcc
     5821 ggtatacctg cgaagcaaca aagcaataag gcaacaacgg caggtaatgc tgctcaaaaa
     5881 agcgtttact gatcccaacg ttcaatatgc gcgtggcacc aaactcataa aaactttgat
     5941 ggtgcgaacc taacggaata aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt
     6001 taatctcctg gaaataacgc ccggttaata ctagagtaaa ttcataatag tcgtgctgat
     6061 gcagtccgct gatactctca gttttgttat agataaacac atggaagttt ttgccattaa
     6121 acaactgctg ttctcgggca gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg
     6181 actccttatg ccttcagttt ttcatgaagc tcaattaatt cagtaatcag ttcacgcgca
     6241 agcatggacg tcattaaatg atcctgagcg tggaccagca ccagactcac tttcatctta
     6301 ccttcgcccg catcgccttc aatcagtttc gtctgtacca gatgcgcttc attcaatgcc
     6361 atccgtgact gatccatcat ggctttcgct gcggcaaaat cgccctgctt cgcctgtttc
     6421 aatgcggcat acgccaggct acgcgcttgt ccagagttga tgataagccc catcaccact
     6481 tcttccagct cttcagcttc tgtttgcgta tcaggaatat tatcgagatc catcatacat
     6541 cgttcctctc tcttcttacc ggcaagatta cccgtaccgg catcgattaa aatttcaggg
     6601 gttagcgata tcttcttcgc tctcttcttt atcaatcgca ttttgtgctt tgttagccac
     6661 cacaacaaag ggcagataaa ttaacgttgc gatgccaagg ttgaagagtg cgaccagcaa
     6721 tgcggcgacg ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg
     6781 tgcaatattg gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc
     6841 cagaatcggt tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag
     6901 accaaacaga atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgccac
     6961 ctgacgataa tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc
     7021 accactgccc ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt
     7081 cttaccggct gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc
     7141 cagcgcccac ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat
     7201 accgaagaac cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa
     7261 cgatgccagt ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt
     7321 attcaacgcc caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc
     7381 cgagaaagaa cgcgatactg aagcaggtac gctgtcgggc agtttaatga cccaattgcg
     7441 gcggacaata aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat
     7501 attcgcccca cctaaccagt ttgcgccaac cgcataggcc tcaccgacac tatatggggt
     7561 gacggtcata aatgctgcaa cggataacaa cccagccgcc agcgcatcga ctttacgctc
     7621 ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattattc ctaatgttcc
     7681 gttatatacg ttgccgccaa tacctttcag accattaagt gtttcaatgg ttgaggcgtc
     7741 gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa aaacgttgtt
     7801 aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt
     7861 gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc
     7921 aagcgatgca ataacattac tcatagaaaa atacccttaa aaaccgcaat ttaaatatta
     7981 cggtattgat ttatgaaata actctttgac gggaaaattt aaaataaatt aatttgctgc
     8041 ggctttttta atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga ctttgccata
     8101 aagcagcgag tcaattactt caaccggttt gttgggtaac aaacgctgga tttcgggcaa
     8161 catataagca atctgcggcc ctaataacac gacatcggca ttctgacctt tttcaccagc
     8221 cagtgtttcc ggaaatgctt caataatgac cggaacttca tatttttctg cctgtgcgcg
     8281 catttttgat accagtaaag agatagacat gcccgcagaa caaaacagat aaatgtgttt
     8341 cttttccata aaactgccct catcgacgat tatctgtcag ccagacactc cgcaagcctt
     8401 gacctgcttc catgctctgg gtaacttgcg aaaccaacat gacttttttt gtggctgaaa
     8461 ggagtatacg gtatagacct gagcaatagt atttctttga ccttataaat tgtttctcct
     8521 tgacctgtgg ttatgaccct cttcacattt cgggcaaata attcgcgaag ataattaaga
     8581 actcaggcca aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa gctttcagac
     8641 gaattgatta cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc tgataaccgg
     8701 agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct ttaccatcac
     8761 gttgacccag gatgtaggtc tggcaagtac cgcgagcatg gatcatgctc acttcagacg
     8821 aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg acgtctttca
     8881 ccacaggctg tacaaactgg tctttggtac gatcataagc tgtacaaccc gccagcatgg
     8941 ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc cgtcctcttg
     9001 tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc ccgtgaacga
     9061 aacggctccg ctttcagagg attcctgtat gacgttttaa ccaccattca gcccgctgtc
     9121 gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt ctgtctggag
     9181 gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa
     9241 tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc aaacttatcc
     9301 gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc ttgccggaaa
     9361 gctgtgccag atggcgatta atgagctgcg ccaggaaact cgcaacgaat cactgcaatt
     9421 tattgccgta cgcctgccct atggtgttca ggctgacgaa caagattgcc aggatgccat
     9481 tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg tattggctag
     9541 cgagcaggca ttgcgggaag caggcattga actgagcgat tttgtccgtg gcaatgaaaa
     9601 agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagcg gtgtcgtggt
     9661 gggcaccgat catgcagcag aagccattac cggattcttc actaaatatg gtgacggcgg
     9721 tacggatatt aatccgctgt atcgtctcaa caaacgccag ggtaaacagt tactgacggc
     9781 attaggttgc ccggaacacc tttataagaa agccccaact gccgatcttg aagatgatcg
     9841 cccttctctg ccagatgaag tggcactcgg cgtgacctat gacaatatcg acgactatct
     9901 ggaagggaaa aacctacctg aacaggtcgc cagaacaata gagaactggt atctgaaaac
     9961 cgaacataaa cgccgtccgc caattaacgt tttcgatgat ttctggaaaa agtaataatt
    10021 tgcacctatt ggattgtgcg aaaaagagta atttgttcac gccggatgcg gcgtgaacgc
    10081 cttattcgac ctacaaaact atgcaaattc aatacattgc aggagtcgaa taggcctgac
    10141 aggcgtagca cgtcagacgg tgtaaccttt gtcatcaacc cgcctctttt ttaatcgcct
    10201 cccgcctgtt acactggata gataaccagc attcggagtc aacagtggta cggcgtttaa
    10261 cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa catttacatt
    10321 cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc gaaagtgaca
    10381 ccatgccgct ctatatcggc aaaagcgtta atatccgcag tcgggtgctt tctcatttac
    10441 gcaccccgga tgaagccgcc atgctacggc aatcccggcg aattagctgg atctgcaccg
    10501 caggcgaaat cggcgctctg ctccttgaag cgcgattaat caaagaacaa cagccgctgt
    10561 ttaataaacg gttgcgccgc aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg
    10621 tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc ccccaacctg tttggcctgt
    10681 ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa caaaaacttt
    10741 gttatggcct gttgggactg gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc
    10801 taaaacgttg cgccggagca tgctgcggta aagagagcca tgaggaacat gcgctacgct
    10861 tgcgccagtc tctggagcgt ttgcgagtgg tgtgttggcc ttggcaaggt gcggtggcac
    10921 tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg ctgtggctgg
    10981 gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc gggtttgatc
    11041 acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa attactgaac
    11101 ttgatccggc gaatgaccag cgagccagtt gatttcactg aacaacagtt taccttgcgg
    11161 ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt cggtagtgag
    11221 cagtttgtcg cccagttgcc acgccccgtt aatcacaaac accaccccgc cgcgtgagcc
    11281 aaaggtagta aacgtacgtt cggcaattct gactttcgcc ttacagacat ccaggcgcgt
    11341 cataatattg aaatccatcg acatctgccc tgccgtcagt ttcgctttta ccacctggtc
    11401 cgccgtaaag gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt ctgcgctttc
    11461 aaggagcatc tcgccgcctt ccagcaacgt cactatcctt tccatgccgg gaaacaaaga
    11521 aaactcccca ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat cacgttttgc
    11581 cggtgggaaa gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca gattcaccga
    11641 cattttacgc atatcaaagt attccatact cgctcccggc ccgttctttt cattgttatc
    11701 gttcaggtaa ccgtggatga ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg
    11761 ctttttttac aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac
    11821 tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaagat tattcagcag
    11881 ttgcaggcat tttacctttt gccgctggac gttctgtcag acgcttctca aaattagcat
    11941 taaattgctt tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg gtttccatgt
    12001 gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct tcagcttttg
    12061 ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcggcgttct tccagcggcg
    12121 gacgtttcat ctggtcacgc tggcctttca tgatttcgtg gatctgctgt ttctgcgcgt
    12181 cggtcaggtt caggtctttg aacatcatgt cctgatgcgg accgaacttg cctttgtggt
    12241 gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt gtctgcggca tgggccaggt
    12301 tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta cgcatattct
    12361 atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt gttgacgaga
    12421 ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa
    12481 aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag tgatgagaga
    12541 atattacaac acgatgattt tgcagagatt atgaagaact ataccggatg actggtgata
    12601 aataaagcaa ataaccagga ttaatctgta ttaatttata agaaagcaac ttaatacccg
    12661 cagaatgatt tctgcgggta agtattagct tattttttcg agcattaatc ccgcgcgtaa
    12721 tcccaacgct accaacggat tagggaataa cacatactct acatcatggg ttacggtaaa
    12781 acgttcctct ccgtcctgcg ccagcaatgt tcctttctca aacggcataa aattcagcgt
    12841 gtcacttgcc atatgcattt cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg
    12901 ataacggagc ggcggtgttc tcacgatacc gacactctca ccagatagca gcgcagcaat
    12961 tgcgctggca gttactgcaa actggcgaag atcgttttgc ccaaagggca acgctttgcc
    13021 aagttccaac gtacagacca gcgcgccaaa atgtctgacg ctgaaatggg taaacgtacc
    13081 accaggctcc tgatggaaca ccagcgcctc cagccccgcc gcacccagcc acgtcagaaa
    13141 tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg aactgcggat gcaaggagcc
    13201 acgaattgcg gtatgtagat caaggtgcca acgcacagat tctttgccct ggtcataaaa
    13261 atcttccagg cactgttcca gttcgcgcgc ccgacaggtt tctccgcttt cagcaaatag
    13321 ctgccaacga ccgccaaaca ttcggttcat atcgctatgg caataacgtt tcccttgctt
    13381 cagcgcagga ggattcccga ggatcaccag caaccgccaa cgtaacggga tctcgccgtg
    13441 agatatcgcg ccaagcaacg cgtccagcat ttccacaggt gccgtctcat taccgtggat
    13501 ccccgcagaa atcaccagcg cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc
    13561 atcgcccagc cagcgccagc gaacgccgtt gatttctcgc tcggtgataa ccggtttttt
    13621 acccgttaag gtcagagcaa gaaaattatc cattgccccc tccctcgcgc tggaacggat
    13681 aaaccgaacc gagattcagt aattggctca atacatccag tgcttcccgc ccttcgcgca
    13741 gcaattgcgg gtcggccaga tcggcagcag taagacgatc gcggtagtaa cgatccaccc
    13801 agtcattgag cacattaaac aaggtatcgt tcatcatcac cgccggattc accgcccggc
    13861 gttcttcttc tgtcaatacc ccccgcaacc gcaggcacgc cgggccgccg ccattcgcca
    13921 tgctttcacg taagtcaaag acttttagtt cactaatcgg gttgtcagcg gcaaggagtt
    13981 cattgagata accccatact ccggcgtgct cccgacactc ctgaggcagc accaacatca
    14041 tggaaccatc atcgcggctc aacagttggc tgttaaacag ataggtagac accgcatccg
    14101 acacggaaac ctgagttgcc ggaacttcta tcgccataaa accgttcacc cgcgtgccag
    14161 taactgtgac tggcgagcga acgcctgttg gtggcaaaac agcacctggc ggttactcac
    14221 ggcaatcacg tcattatgaa aaacgctctg gtcgataacg tccgggtttt gctgggcgaa
    14281 aatcacctgt tggggattca cctgattaag ccttgccacc gcctcgctgg cttcgcgagt
    14341 ctgtcgcgcc ggataacggg aaggccgggt atcattgcct tcttctcgcc cgtagacaaa
    14401 aagtagcata cccggttcac cgtaatgacc gccgagacga ttgtggtttg ccgccccctc
    14461 atcaccgagc aacgctacct gcggcaacgc cgaatggacg ctaaatttct cttcgtcctt
    14521 aaaaatcgct tttaacagcg attcagtgac gggcgcttcc agcgaacggt gaaatttatt
    14581 gttcaggttg gcaacggtga gatgcacttt gccatccagc gtatcggcag atggcgcgat
    14641 cgttgccgca ttggctaccc acattggcga agcggaactg acgctggaaa gccagtgcgg
    14701 tgcctggcgt gcaacttttt ccagtacctg ctcatcgcta ccactgaatc ccaactgacg
    14761 cagcaccgga ataaacggac gctcgtgcgg cgggatcacg gcctggggga atcccgcatc
    14821 ggcaagggct ttcattttca gtaagccctg ttttgccgcc agtcgcgggt tagacacctg
    14881 aaaacggtga cgggtagagg cttcattacc aaacgacagg cccgcgtaat gatgcgtcag
    14941 ccctaccagc ccgtcgaaat taacttccca ggcgttcatc gcaccacctc atcggaaaaa
    15001 tccagcccgg ggttaagcgt ggcgggcaat gttaacgagt ccgactccag gctcgccata
    15061 ggccatgcgc agtaatctgc ggcataccag gcgctggggc gatggttacc ggaagcacca
    15121 atgccgccga atggggcggt actggcagca ccagtaagcg gtttgttcca gttaacaatc
    15181 cccgcccgcg cctccagcaa cagttgatcg aatttttccc gctcggggga aaccagaccg
    15241 caaaagagtc cgaagcgagt gttgttcgcc atccgaatcg cctcatcaaa agtatcataa
    15301 cgccagacgc gcaaaagcgg cccgaacacc tcttcatccg gcacaccgcc aacgcccgtc
    15361 atttcaatga tccctggtgt cagcaacgat gtccctgctt gtaataagcg cggcgcaagc
    15421 agggttcgtc cgcccatcgc ttccagttgc tgccaggcag taaccacctg ctgtgcggcc
    15481 tgttcagaaa tcagcccgcc aataaacggc tgcggttcgt catcccagtt gcccggcgtt
    15541 aatcgctggc tgacggcaac cagactagca agaaacgcat cgccctgcgc tccgcttttg
    15601 agcaataaac ggcgggcgca ggtgcagcgt tgcccgactg tgacaaacgc cgactgaatg
    15661 gtcagatgga cagccgcgtc gatatccgcc acctcatcga taattagcgg attattaccg
    15721 cccatctcaa gggcgagaat tttctccggc tgaccggaga gctggcgatg cagctggtag
    15781 ccggtattgg cgctaccggt aaacagcaaa ccgtcgagat cctccagcgc actcagcgcc
    15841 tgacccgttt cacgcccgcc ctgcaccagg ttcagcacgc ccggcggcaa gccagcctgc
    15901 tgccataaac gcattaccgc ttcgccactc cacggtgtca gttcgctggg tttaaagata
    15961 atggtgttac ctgccagcaa tgccggaacg atatgtccgt tcggcaaatg accagggaaa
    16021 ttatacggcc caaacaccgc cagcacgccg tgcgggcgat gtcgcaggct cgccgcaccg
    16081 tccggcattt cactacgctg ctcgccggta cgaacgtgat acgccttaat tgatatcgcg
    16141 attttattga tcatcgccgt cacttcggtt gccgcttccc agcgcggctt acccgtttct
    16201 ctggcaataa tcgcggttaa ttcggcttta ttgctttcca gcaatccggc aaagcgttcg
    16261 acaacgacct gacgttcagc caatgagagc cgcgcccagc gcggaaacgc cgcacgggct
    16321 gcccgacaag cctgttcgac ctgagcggca tcggcatcat tgccttgcca taacacctcg
    16381 cccgataccg gattacgctt cacacgcgat gcgccctggc ccgttatcca gtcaccgtta
    16441 atccataaag tcatgctgtt ttctcctctg cgcacaggcg caccagacga acgcgatccc
    16501 ctgcgtggca tttgagggca tccagttgtg cggcggttaa aatcaaacgc tcggttgccg
    16561 gatcggtacg cgccagcacc acgcggaaat ggtgataatt ttcattggcg accaggcagg
    16621 ccggaaaatc gccctgcgca ggctgccctt ctgccacttc caccagccga cttttacgga
    16681 tggcgcgcac gcggtcgatg tcacactcaa gcgttggccc gccgtcgaag atgtcgatat
    16741 agttgcggta acgaaaacct tctttctcca gcaccgcgcg ggcaggcgcg gtttgcggat
    16801 gtacctgacc gatgacgtcc tgggcttcct gggataaaaa gtgggtatag atcggatgtt
    16861 tcggcatcag ttctgcaata aatgcctttt gcccggtgcc gcagagaaaa tcggcgcggc
    16921 taaaatccat cgaaaagaag cgtttaccga ggctttgcca gaacggtgaa tagccgtgtt
    16981 cgtcaatcac cccgcgcatt tcagcaacca ctttgtcatt aaacttgtcg cgaaaagccg
    17041 ccataaacat aaagcgcgat ttcgacagca aatagccgtt gccctctttg cgccagtccg
    17101 ggtcgagaaa cagcgtgcac agctcgctgc tgccggtgtg atcgttacta agaaacagcg
    17161 tcggcaatgc gttatagaca ttcagctctt ttgaggcgtg aaccaacgtg ccgacgcgat
    17221 agttgtacca gggatcgttc agcccaaccg ccacctcaat agcgcaaatc cccgccacgg
    17281 tgcctgtctc gctatcttcc agcacgaaca catagccctg ctcgcttttg ggcagttcac
    17341 cttgccaggt tttgattgcc ctttcgatac gcgccgaaag cgtggcttca ttggcgggaa
    17401 gcgacgtcag gccgccgccc gttttgctgg caagctgcat cagcgccgag acatctgagc
    17461 gctcaacggg acggatgacc atcatgatga acctcggcta acaaagtgtt cgcaagcagc
    17521 tgcaaagcga tccagtccgg tcgtcacctc ttcttcgctg acattgagcg caggcgcaaa
    17581 acgcaccacg ttgccacccg caatcagtac catcacgcct gctttcgccg cttccagaga
    17641 gatctgtttc gcttgcccgg cgtaatcggc attcagtaca cagccaatca acaaacctaa
    17701 gccgcgaact tcactgaaca aaccatagcg gtgattaata gtattaagac gctcaacaaa
    17761 ccagtcgtga cgctgtttaa cgccattaag catctctggt gtgttgatga gctccagcac
    17821 tttgcctgct accgccgagg ccagcggatt accgccatag gtggtgccat gagtgccaac
    17881 ggtcatcacg cgggcgcact cttcggttgc caacaacgca ccgaccggga aaccgccgcc
    17941 cagcgctttg gcggtggtta acagatcggg cgtcacgccg tagtgcatat aggcatacag
    18001 ctcaccagtg cgcccgacgc cggtttgtac ctcatcaaaa atcagcaagg cattgtggcg
    18061 gtcacacaat tcacgcagac cttgtaaaaa cgcgttgctg gctggcacca caccgccttc
    18121 gccctagatg ggctcgacaa tcaccgcaca ggtggcgtcg tcaatcagcg cgctggcaga
    18181 gttaatatcg ttatatgcag catgacgaat atccgccggc agtggcgcaa aatcctgtga
    18241 ataggctggc tgcccacccg cactgacagt aaacagcgtg cgaccatgaa acgcattttt
    18301 gaacgccacg atgccactct tatggctgcc gtagcggtcg tgagcgaatt tacgcgccag
    18361 ttttagcgcc gcttcgttgg cttccgcacc ggagttacaa aagaagacgc gatcggcaaa
    18421 cgtggcgtcg atcaattttt tcgccagtcg cagtaccggc tcgttggtgt aaccgttgcc
    18481 ggtatgccag aacttactcg cctgttcgtt cagcgcttca cgcagttccg gatgcgcatg
    18541 gcccagcgcg ttcaccgcaa tgccacccgc gaagtcgata tactctttcc cctgctgatc
    18601 ccacaagcgc gaaccttcgc cacgtaccgg tataaagggt gccggagcgt aaacaggtat
    18661 catccattca tcaaagtttt cacgcgtaat tggctgagac atagcgacct ctacagtaaa
    18721 taattcgtta tttgcatgtt aacaataagt aatgtttgcg ctgtaaatgt agattgcagg
    18781 gttcgtgcca gccagtgata aaagtgcata aacggcggag gctaactgga aatcaaggag
    18841 ttataaccaa accatatgca tttaaagtgc atataaagtg aatacgtttg cgatgtgggt
    18901 gaataaaaag aataaaaaac gcaatgttat gcagaagtaa aatataattc tagaattgtg
    18961 atcattgacg aaatttactg gaaattactg cgccattctg acgcagcgcg caccaaaagc
    19021 gggcattttt tgcgccatca ttgacatcat taacaaccat cgatcaaatc acttaacaac
    19081 aggcggtaag caacgcgaaa ttctgctacc atccacgcac tctttatctg aataaatggc
    19141 agcgactatg aaatttgtct cttttaatat caacggcctg cgcgccagac ctcaccagct
    19201 tgaagccatc gtcgaaaagc accaaccgga tgtgattggc ctgcaggaga caaaagttca
    19261 tgacgatatg tttccgctcg aagaggtggc gaagctcggc tacaacgtgt tttatcacgg
    19321 gcagaaaggc cattatggcg tggcgctgct gaccaaagag acgccgattg ccgtgcgtcg
    19381 cggctttccc ggtgacgacg aagaggcgca gcggcggatt attatggcgg aaatcccctc
    19441 actgctgggt aatgtcaccg tgatcaacgg ttacttcccg cagggtgaaa gccgcgacca
    19501 tccgataaaa ttcccggcaa aagcgcagtt ttatcagaat ctgcaaaact acctggaaac
    19561 cgaactcaaa cgtgataatc cggtactgat tatgggcgat atgaatatca gccctacaga
    19621 tctggatatc ggcattggcg aagaaaaccg taagcgctgg ctgcgtaccg gtaaatgctc
    19681 tttcctgccg gaagagcgcg aatggatgga caggctgatg agctgggggg tggtcgatac
    19741 cttccgccat gctaatccgc aaacagcaga tcgtttctca tggtttgatt accgctcaaa
    19801 aggttttgac gataaccgtg gtctgcgcat cgacctgctg ctcgccagcc aaccgctggc
    19861 agaatgttgc gtagaaaccg gcatcgacta tgaaattcgc agcatggaaa aaccgtccga
    19921 tcacgccccc gtctgggcga ccttccgccg ctaatttagc agctctcctg gctcaaactg
    19981 ggtcaggaga attaaccttg agaaaaatca acaaactgtc agtaatgatt tgttgcctgc
    20041 cgtcctttgt tataccgtca ctgcgttttt agttgtctga ccacttctct attatcaagt
    20101 ttgatatagg aaactccacg atgaacgctg agcgtaactt tctgtttgcc tgtcttattt
    20161 ttgcgctggt catttacgct atccacgctt tcggtttatt cgatctgctc accgatttac
    20221 cccacttaca gacactcatt cgccagagcg gattgttcgg ttatagcctc tatattctgt
    20281 tattcatcaa tgccccctct ttctgttacc aggaagcata ctggtgatcg ctggaggaat
    20341 agtttttggc ccgctcttag gacacaactc tcattaattg ccgcaacgct ggcctcctcg
    20401 tgctcattcc tgctggcgcg ctggctggga cgtgatttac tgctgaaata cgtgggccat
    20461 agccatacct tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt
    20521 ctgacccgct taatcccgtt gtttccttac aatattcaaa attacgctta cggattcacc
    20581 accatcgcct tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt
    20641 atttataccg tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta
    20701 caactctgtc tggcgggcct ggcgctgttt attctcatcc agctcgcaaa actctacgcc
    20761 cgacacaaac atgtggatct gtctgcttcg cgccgcagcc cacttactca cccaaaaaat
    20821 gaaggataga acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact
    20881 ctgcttactg gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa
    20941 agtggaatac gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac
    21001 ggtaaccatg ctggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat
    21061 tgtgtttaaa gatggctcca acggacataa atcgctgttt atggcttatg cgcccccgaa
    21121 agcattttat gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga
    21181 taataaagaa acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg
    21241 ggcggcaaaa gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga
    21301 catgcgcttt ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg
    21361 tctggatagc tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga
    21421 aaaacacggc gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct
    21481 ggcaacggtt acctttaaaa tcaccgaata aagccaagat aaaggatgat gaagatacaa
    21541 tcgcgtaaaa tctggtacta ccgtataacc ctcatcatcc tgttattcgc tatgctgctc
    21601 gcatgggcgc tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc
    21661 gccgtggacc a
//
LOCUS       DAJTUN010000063        21658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTUN010000063 DAJTUN010000000
VERSION     DAJTUN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.063"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..449
                     /locus_tag="OO470_002236"
     CDS             <1..449
                     /locus_tag="OO470_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6251467.1"
                     /translation="GLRGRAQNEKISRMSEAFGLKSIASHATDELPLGFKQRLALACS
                     LMHEPDILFLDEPTSGVDPLTRREFWLHINSMVEKGVTVMVTTHFMDEAEYCDRIGLV
                     YRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQLIHDWDKEHSNE"
     gene            442..1575
                     /locus_tag="OO470_002237"
     CDS             442..1575
                     /locus_tag="OO470_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6251468.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            1586..2692
                     /locus_tag="OO470_002238"
     CDS             1586..2692
                     /locus_tag="OO470_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6251469.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(2654..3064)
                     /locus_tag="OO470_002239"
     CDS             complement(2654..3064)
                     /locus_tag="OO470_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6251470.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            3197..3958
                     /locus_tag="OO470_002240"
     CDS             3197..3958
                     /locus_tag="OO470_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6251471.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3955..5196
                     /gene="clsB"
                     /locus_tag="OO470_002241"
     CDS             3955..5196
                     /gene="clsB"
                     /locus_tag="OO470_002241"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6251472.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            5196..6152
                     /locus_tag="OO470_002242"
     CDS             5196..6152
                     /locus_tag="OO470_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6251473.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(6188..6901)
                     /locus_tag="OO470_002243"
     CDS             complement(6188..6901)
                     /locus_tag="OO470_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6251474.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(7106..7810)
                     /locus_tag="OO470_002244"
     CDS             complement(7106..7810)
                     /locus_tag="OO470_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6251475.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(7947..8399)
                     /gene="moaE"
                     /locus_tag="OO470_002245"
     CDS             complement(7947..8399)
                     /gene="moaE"
                     /locus_tag="OO470_002245"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6251476.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(8401..8646)
                     /gene="moaD"
                     /locus_tag="OO470_002246"
     CDS             complement(8401..8646)
                     /gene="moaD"
                     /locus_tag="OO470_002246"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6251477.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(8639..9124)
                     /gene="moaC"
                     /locus_tag="OO470_002247"
     CDS             complement(8639..9124)
                     /gene="moaC"
                     /locus_tag="OO470_002247"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6251478.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(9127..9639)
                     /gene="moaB"
                     /locus_tag="OO470_002248"
     CDS             complement(9127..9639)
                     /gene="moaB"
                     /locus_tag="OO470_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6251479.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(9661..10650)
                     /gene="moaA"
                     /locus_tag="OO470_002249"
     CDS             complement(9661..10650)
                     /gene="moaA"
                     /locus_tag="OO470_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6251480.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(10638..10782)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11047..11955
                     /gene="yvcK"
                     /locus_tag="OO470_002250"
     CDS             11047..11955
                     /gene="yvcK"
                     /locus_tag="OO470_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6251481.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(12147..14168)
                     /gene="uvrB"
                     /locus_tag="OO470_002251"
     CDS             complement(12147..14168)
                     /gene="uvrB"
                     /locus_tag="OO470_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6251482.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(14747..15424)
                     /gene="bioD"
                     /locus_tag="OO470_002252"
     CDS             complement(14747..15424)
                     /gene="bioD"
                     /locus_tag="OO470_002252"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6251483.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(15417..16172)
                     /gene="bioC"
                     /locus_tag="OO470_002253"
     CDS             complement(15417..16172)
                     /gene="bioC"
                     /locus_tag="OO470_002253"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6251484.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(16159..17313)
                     /gene="bioF"
                     /locus_tag="OO470_002254"
     CDS             complement(16159..17313)
                     /gene="bioF"
                     /locus_tag="OO470_002254"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6251485.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(17310..18350)
                     /gene="bioB"
                     /locus_tag="OO470_002255"
     CDS             complement(17310..18350)
                     /gene="bioB"
                     /locus_tag="OO470_002255"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6251486.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            18437..19726
                     /gene="bioA"
                     /locus_tag="OO470_002256"
     CDS             18437..19726
                     /gene="bioA"
                     /locus_tag="OO470_002256"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6251487.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            19785..20261
                     /locus_tag="OO470_002257"
     CDS             19785..20261
                     /locus_tag="OO470_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6251488.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(20666..20752)
                     /locus_tag="OO470_002258"
                     /pseudo
     CDS             complement(20666..20752)
                     /locus_tag="OO470_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            20844..>21658
                     /locus_tag="OO470_002259"
     CDS             20844..>21658
                     /locus_tag="OO470_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6251489.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
BASE COUNT         5089 a         5828 c         5474 g         5267 t
ORIGIN      
        1 atggcttacg cggtcgggcg cagaacgaaa aaatctcccg tatgagcgag gcatttggcc
       61 tgaaaagtat tgcctcacac gccaccgatg aactgccatt aggttttaaa cagcggctgg
      121 cgctggcctg ttcgctgatg catgaaccgg atattctctt tctcgacgaa ccgacttccg
      181 gcgttgaccc cctcacccgc cgtgaatttt ggctgcacat caacagcatg gtagagaaag
      241 gcgtcacggt gatggtcacc acccacttta tggatgaagc ggaatattgc gaccgcatcg
      301 gcctggtgta ccgtgggaaa ttaatcgcca gcggtacgcc ggacgatttg aaagcacagt
      361 cagctaacga tgagcaaccc gatcccacca tggagcaagc ctttattcag ttgatccacg
      421 actgggataa ggagcatagc aatgagtaac ccgatccttt cctggcatcg cgtgcgggcg
      481 ctgtgcgtca aagagacgcg gcagatcgtt cgcgatccga gtagctggct gattgcggta
      541 gtgatcccac tgctactgct gtttattttt ggttacggca ttaacctcga ctccagcaag
      601 ctgcgggtcg ggattttgct ggaacagcgt agcgaagcgg cgctggattt cacccacacc
      661 atgaccggtt cgccctacat cgacgccacc atcagcgata accgtcagga actgatcgcc
      721 aaaatgcagt cggggaaaat tcgcggtctg gtggttattc ccgtggattt cgccgaacag
      781 atggagcgcg ccaacgccac cgcaccgatt caggtgatca ccgacggcag tgagccgaat
      841 accgctaact ttgtgcaggg gtatgtcgaa gggatctggc agatctggca aatgcagcga
      901 gcggaggaca acgggcagac ttttgaaccg cttattgatg tacaaacccg ctactggttt
      961 aacccggcgg cgataagcca gcactttatt atcccgggtg cggtgaccat tatcatgacg
     1021 gtcatcggcg cgattctcac ctcgctggtg gtggcgcgag aatgggaacg cggcaccatg
     1081 gaggctctgc tctctacgga gattacccgc acggaactgc tgctgtgtaa gctgatccct
     1141 tattactttc tcgggatgct ggcgatgttg ctgtgtatgc tggtgtcagt gtttattctc
     1201 ggcgtgccgt atcgcggttc gctgctgatt ctgtttttta tctccagcct gtttttactc
     1261 agtaccctgg gaatggggct gctgatttcc acgattaccc gcaaccagtt caatgccgct
     1321 caggtcgccc tgaacgccgc ttttctgccg tcgattatgc tttccggctt tatttttcag
     1381 atcgacagta tgcccgcggt gatccgcgcg gtgacgtaca ttattcccgc tcgttatttc
     1441 gtcagcaccc tgcaaagcct gttcctcgcc gggaatattc cagtggtgct ggtggtaaac
     1501 gtgctgtttt tgatcgcttc ggcggtgatg tttatcggcc tgacgtggct gaaaaccaaa
     1561 cgtcggctgg attagggaga agagcatgtt tcatcgctta tggacgttaa tccgcaaaga
     1621 gttgcagtcg ttgctgcgcg aaccacaaac ccgcgcgatt ctgattttac ccgtgctaat
     1681 tcaggtgatc ctgttcccgt tcgccgccac gctggaagtg actaacgcca ccatcgccat
     1741 ctacgatgaa gataacggcg agcattcggt ggaacttact caacgttttg cccgcgccag
     1801 cgcctttaca catgtgctgc tgctgaaaag cccacaggag atccgcccga ccatcgacac
     1861 acaaaaggcg ttactactgg tgcgtttccc ggctgacttc tcgcgcaaac tggatacctt
     1921 ccagaccgcg ccgttgcagt tgatcctcga cgggcgtaac tccaacagtg cgcaaattgc
     1981 cgccaactac ctgcaacaga tcgtcaaaaa ttatcagcag gagctgctgg aaggaaaacc
     2041 gaaacccaac aacagcgagc tggtggtacg caactggtat aacccgaatc tcgactacaa
     2101 atggtttgtg gtgccgtcgc tgatcgccat gatcaccact atcggcgtaa tgatcgtcac
     2161 ttcactttcc gtcgcccgcg aacgtgaaca aggtacgctc gatcagctac tggtttcgcc
     2221 gctcaccacc tggcagatct tcatcggcaa agccgtaccg gcgttaattg tcgccacctt
     2281 ccaggccacc attgtgctgg cgattggtat ctgggcgtat caaatcccct tcgccggatc
     2341 gctagcgctg ttctacttta cgatggtgat ttacggttta tcgctggtgg gattcggtct
     2401 gttgatttca tcactctgct cgacacaaca gcaggcgttt atcggcgtgt ttgtttttat
     2461 gatgcccgcc attctccttt ccggttacgt ttcgccggtg gaaaacatgc cagtgtggct
     2521 gcaaaatctg acgtggatta accctattcg ccactttacg gacattacca agcagattta
     2581 tttgaaggat gcgagtctgg atattgtgtg gaatagtttg tggccgctac tggtgataac
     2641 ggccacgaca gggtcagcgg cgtacgcgat gtttagacgt aaggtgatgt aacttcttat
     2701 ctttcgccag caaagacact accgccgggc cagcaaggat cgccagccct gcaattgcca
     2761 gcaaaaagtt gttttccagt acgcgggaca acagccacag cacaatcagc gctgcgccaa
     2821 aataccaggt cgtggtcagc tcttccagca cgtctgccac ctcacgccag cgttgctcgg
     2881 ggtggcgctg aaacaccagc atcatcacta ccgtgacaca atacagtgcg cataatccca
     2941 cgccgacgtg aaccagagtc ttactcatcc agcccaccac cagcagcgcc actaccagta
     3001 aatgcaacat aatctgccag caacttagac ccgttgcgac acgaacacgt tgttgccact
     3061 tcatggcttc tctcctgaag aatttatctc tacttactca gagtaccgaa ctttacggaa
     3121 aattccgtaa gaccgcacct ttttgtgacg ccgactacac tatttcaatg agataagagg
     3181 caaagacagg aaaattatgc ccgatcaaac acaacaattt tcgttcaagg tgctcaccat
     3241 caacattcac aaaggcttta ccgcgtttaa ccgacgcttc attttgccgg aacttcgcga
     3301 tgccgtgcgt accgtcagcg ccgatattgt ttgcctgcag gaagtgatgg gcgcgcacga
     3361 agttcatccg ctgcatgtgg aaaactggcc cgatacctcg cactacgagt ttctcgccga
     3421 cactatgtgg agcgattttg cctacggtcg caatgccgta tacccggaag ggcatcacgg
     3481 caacgccgta ctgtcgcgtt atcccattga acattatgag aatcgcgatg tttcggtcga
     3541 tggtgcggaa aagcgcggcg tgctctactg ccgcattgtg ccgccgatga ccggaaaagc
     3601 gattcatgtg atgtgcgtac atctgggcct gcgtgaggcg caccgtcagg cgcagcttgc
     3661 gatgctcgcc gaatgggtga atgagctacc ggacggcgaa ccggtattgg tggcgggtga
     3721 tttcaatgac tggcggcaaa aagctaatca tccgttaaaa gtgcaggccg gactggatga
     3781 gatttttacc cgcgcccacg gacgcccggc gcgcacgttt ccggtgcaat ttcctctact
     3841 acgactggac aggatctacg tcaaaaatgc cagcgccagc gcgccaaccg cgttgccgct
     3901 gcggacatgg cgacaccttt ctgatcatgc ccctttaagt gcggagattc atttatgaaa
     3961 tgtagctggc gcgaaggcaa taagatccag ttgctggaaa acggcgagca atattatccc
     4021 gcggtgttta aggcgattgg cgaggcacaa gaacgcatca ttcttgaaac gtttatctgg
     4081 tttgaggata acgtcggcaa acagctgcat gcggcactac tggcagcagc gcaacgcggg
     4141 gttaaagcgg aagtcttgct ggatggctac ggttcgccgg atctcagcga tgagtttgtc
     4201 aatgaactga cggcagctgg cgtagtgttc cgctactacg atccccgccc tcgccttttt
     4261 ggtatgcgca ccaatgtgtt tcgccggatg catcgcaaaa ttgtggtgat cgacgcgcgt
     4321 atcgccttta ttggcgggct gaattactcc gccgagcata tgtccagcta cggtccagag
     4381 gctaaacagg attacgcggt acgccttgaa gggccgattg tcgaagatat tctccagttt
     4441 gagctggaaa acctgcctgg acagagcgcg gcacgacgct ggtggcgacg tcatcacaaa
     4501 gcggaagaga accgccagcc gggagaagcg caggtattgc tggtctggcg cgataacgaa
     4561 gaacatcgcg atgatattga acgccattat ctgaaaatgc tcactcaggc gcagcgggaa
     4621 gtgattatcg ccaacgccta cttcttcccc ggctatcgat ttttacacgc cttgcgtaaa
     4681 gcggcacggc gcggggtgcg gatcaaactg atcattcagg gcgaaccgga tatgccgatt
     4741 gtcagagtcg gtgcgcgctt gctgtataac tatctggtta aaggcggcat tcaggttttt
     4801 gagtaccgcc gccgcccgct ccacggcaaa gtggcattga tggtcgatca ctgggcgaca
     4861 gtagggtcca gtaatctcga tccgctcagt ttgtcactga atctcgaagc aaatgtcatc
     4921 atccacgatc gtcattttaa ccagacgctg cgcgataatc tgaacggcat tattgccgca
     4981 gattgtcagc aggtggatga aaccatgctg cccaaacgca cctggtggaa cctgaccaaa
     5041 agcgtgctgg cgttccactt tttacgccac ttcccggcgc tggttggctg gcttccggca
     5101 cacacaccac gtctgacgca ggttgatccc cccgcacaac cgacaatgga aacgcaggat
     5161 cgggtagaaa ctgaaaacac gggggtaaac ccctgatgag taaatcacac ccgcgctggc
     5221 gcttagcaaa gaagatcctc acctggctgt tttttatcgc ggtgattgtg ttgctggtgg
     5281 tctacgccaa aaaagtggac tgggaagagg tctggaaggt catccgcgac tacaatcgcg
     5341 ttgcgctgct tagtgcggtc gggctggtgg tcgtcagcta tctgatttac ggctgctatg
     5401 acctgctcgc ccgcttttac tgcggtcaca aactggcgaa gcgccaggtg atgctggtgt
     5461 cgtttatctg ctacgccttc aacctgacgc tcagtacctg ggtcggcggc attggtatgc
     5521 gctatcgttt gtactcccga ctagggttac cgggcagcac tattacgcgg atttcctcgc
     5581 tcagtattac caccaactgg ctgggctata ttttactggc agggattatc tttaccgcag
     5641 gcgtggtgga gttgccggat tactggtatg tcgatcaaac tacgctgcgt attctcggca
     5701 ttggcttact gatgattatc gcggtttatt tgtggttttg cgctttcgcg aagcaccgcc
     5761 atatgaccat caaagggcaa aagctggtgc tgccttcatg gaaattcgcc ctcgctcaga
     5821 tgctgatttc cagcgttaac tggatggtaa tgggggcgat tatctggctg ttacttggtc
     5881 aaagcgtgaa ctatttcttt gtactgggcg tgttactggt tagtagtatt gctggcgtca
     5941 tcgtgcatat tccggcgggg atcggtgtgc tggaagcggt gtttatcgca ctactggctg
     6001 gggagcatac ctcgaaaggg agcattatcg ccgccctgct cgcttatcgc gtcctgtatt
     6061 actttatccc gctgctgctg gcgctgatct gttacctgct actggaaagc caagcgaaga
     6121 agctacgggc gaaaaatgaa gcggcgatgt gaagtttagt taagttcttt agtatctgca
     6181 tttacgacta atgaaaaaaa cgcgtatgcc tttgccagac aagcgttata gctatcgcgg
     6241 caagtatccc aaagaagccg ataaaatagt aatagagtgt taatgcacct gtaaaagcgc
     6301 aacgaaccgg actccacaac tcttcactat ggcattttcc tgcctgttca aggagcttat
     6361 ttcgtccatg cgagataatg cctggccata acacaatcat taagcaactt attacccaaa
     6421 tgggccgttc actcattaat gccgcattca cgatgattac cagggccaac cctgtcaacg
     6481 tcatcataat gataaaacgg tgagaaccag gatcaacatt aaataataaa ccggccagca
     6541 tactgattaa aaacatcgcg cccgcagtgc tgaacacgat gataatggca tatacaggaa
     6601 ctaaacttgc aacgacaatc gccaccaaag agagtaaaaa cagatatatt acgcccgcga
     6661 tggcggcatt tatcttgttg atatccttac ttaagccata catacaaagt aataataacc
     6721 ctatggcgac atacattggt ggtgaatcat atttggttgc cgtaatttcg agatacttag
     6781 ctccccaggc tagtcctgcg gttgccacca aacctaaagc ggtaatgagc catataccgt
     6841 ttaatatcct ggcctcgtgc tgaaaatcta atttattgct gttttgcgag tatgattcca
     6901 ttcttttagt tgtccttaga aaaaacatta atattcacca tcaataaatt taaaaccacg
     6961 catacaaaat acttttgtat ttatggatac ggaaaacgat tccgctcgct cataaggcat
     7021 attttatacc tggtacttgt taagtgtcgt ttttagtaag aaaaaaatcc cgacacatgg
     7081 ccgggatagt gatggtgaaa aagaattaac ggcggttgcc gaagatccgt aacaacatca
     7141 ggaacaggtt gatgaagtcc agatacaagg ttaacgcacc aagaatggaa tatttgcgca
     7201 ggttcgacgt gtcgcgggta tcaatctgct cacccatatt tttcaatttc tgagtgtcat
     7261 acgccgtcaa tccgacaaag acaatcacgc cgatgtaggt aactgcccac atcaatgctt
     7321 cgcttttcag ccagaagttg accagcgatg ccagcacaat gccgattaac gccataaaca
     7381 gcatattgcc aaagccactt aaatcgcgct tcgtggtgta accgtacagg ctcattgcgc
     7441 cgaacatccc ggcagtaacg acgaaagtac tggcgataga agcagcggta tagacaatga
     7501 atatactgga aagcgtaaga cccgtcagcg ccgaataaag cataaagagc atcgtcgtta
     7561 cacctgcgct cagcttttga atcatcgctg ataacacaat aaccaatgct aattgcgcga
     7621 tgatcagacc gattaaaaag acacggttag tgaacaacag ctccatcacg gccgcggaat
     7681 tagccgcata ccaggcaaca aatgcggtca gcaacaagcc aacggtcatc cagccataga
     7741 cttgcgccat ataagtttgc aagccagccc ggggttgtac gattgaatca gaacgtggga
     7801 atctgtccat gacgatctcc tgaagatata aggaatatct taaggatact gcaaaatgat
     7861 gaggctgtgc atcgacgcag cgtaaacgca tgtactgagc ggtgaaattg ccggacgcag
     7921 cggtgcctta tccggctaac aaaaaactac cagcgttttg ccgcctgcag atcgctctcc
     7981 cgggcttcaa cccagcggtc gccttccggc gtggcttcgc gcttccagaa cggtgcgcgg
     8041 gttttgagat aatccataat aaactgcccg gcttcaaacg cactgctgcg atgcgcactg
     8101 gtgacaccga caaaaacgat ttcatcgccc ggccataatt ccccgatgcg gtgaatcacc
     8161 gtgacgcgcc ccagcggcca gcggttacgc gcttcatcaa caatttctgc cagtgctttt
     8221 tcagtcatcc ccggatagtg ttcgagggtt aatgctttga cgctgtcgcc caggttatgg
     8281 ttgcgcacct ttccggtgaa ggtgactacc gcaccgtctt cgtcacgctc cgccagccac
     8341 gggtactctt ctcctacgct gaacggctgc gggccgacaa caattttggt ttctgccatc
     8401 ttaacctccg gttaccggcg ggaagaaagc tacttcgtcg ccgtcagtca gcggatggtc
     8461 aaaactcacc agcgtctggt tgacggcagc cagcaatttg ccatcttcca gcgccagcgc
     8521 ccagcgatcg ctctgcgcag ccaggtgctg gcgtaacgct tcaacggttg ggtaatccgc
     8581 agccacttcg gttgcatctg ttcccaccaa ctcgcgcacc tgggcgaaaa aaagaacttt
     8641 aatcatccgc ttccacctta aagtcacccg acttgccgcc gctcttcgcc agcaaacgta
     8701 ccggaccaat caccatatct ttttgcaccg ctttgcacat gtcataaatg gtcagcgccg
     8761 ccacggaggc cgcggttaac gcttccattt cgacaccggt tttcccggtc aggcggcata
     8821 aggtttctat acgcacccga ttgtgctccg gctcggcctg taaattgact tcaactttgc
     8881 tgagcatcag cgggtgacac agcgggatca gatcccaagt gcgttttgcc gcctgaatac
     8941 cggcaatacg cgcagtggca aatacgtcgc ctttgtggtg gcgaccatca ataatcatcg
     9001 ccagcgtctc gctgcgcatg gtgacaaagg cttccgcccg cgcttcacgc acggtttccg
     9061 ctttggcgga gacatccacc atgtgcgctt cgccagcggc gttgatatgg gtcagttgcg
     9121 acatacttat ttcttcaaat gtggatggaa attacacgga cgcgtacggg catccagctg
     9181 cggcgcgatg atattttccc atgcggtacg gcacgctttg gtcgaacccg gcatggcgaa
     9241 aatcagcgtt ttgttagcga cgcccgctac cgcacgagat tgcaacgtgg aagtgccaat
     9301 ctcttcaaac gacaacatac ggaacacttc accaaaacct tcaacctcac ggtcgaacaa
     9361 cggcagcagc gcttcgggag cctgatcacc ttccgtcagg ccagtaccac ccgtaatcaa
     9421 taccacttgt acatcgtcgc tggcgatcca cgcagatacc tgagcgcgaa tagcgtagcg
     9481 gttttctttc acaatggctt catcgacaac gtgatggccc gcttcttgcg ccgaatcgcg
     9541 cagatagtga ccggaggtat cgtcttcttc accgcgacga ttagaaaccg taagaatagc
     9601 aatacgggtc gggataaatt cagtgcttac ctgactcatc tgatctctcc ttttgacgtt
     9661 ttagccgcca atgtacgata agttttgcgt aataccggtg ttgttttgat gcaggaaatg
     9721 ggtctgtttc ttctcccgca gcgccgctga aatacgcgct tccagcgcct gttgctgggt
     9781 atcgccttcc agcagatcgc gcaggttaac gccgccttca ccaaacaggc agagatggag
     9841 tttaccaatg gaggaaacgc gcaggcggtt gcaagtggcg cagaagtctt tttcatacgg
     9901 catgataagg ccaatctctc cggcgtaatc tggatggcaa aagacttgcg cgggaccgtc
     9961 gctgcgttga cgtaattggt ggatccagcc gcgacgcagt agctcgtcac gcagaacctg
    10021 accagagatg tgatgcttac ggaagagctc gctgccctcg cccgtttcca tcagttcgat
    10081 gaaacgcagc tggatagggc gatgctggat ccagttcaga aaggtgtcga gctggtgatg
    10141 attaacatca cgcatcagca cggtattgac tttgaccttc tcaaaaccgg cctcaaatgc
    10201 agcatcaatc cctgccatga cctggttgaa tttatcctgc ccggtaatag cgtgaaactg
    10261 gcgggcgtcc aaactgtcga cactgacgtt aatgccagta agtcccgcat cgcgccagtt
    10321 cgccacatcg cgttccagac ggtaaccatt ggtggtgacc gcaatctggc ggatagcgtc
    10381 gttttcccgc acagcggcga tgatatcggt aaagtcgcgg cgtaaagacg gctctcctcc
    10441 tgtcaggcgc actttttcgg tgcccagact ggcgaaggcg cgcgtaaccc ggcgaatttc
    10501 atcgacggta agaaagcctt tattggtgac gccgctcggt ttgtagccat ccggcaggca
    10561 gtaggtgcaa cgaaagttac acacatcggt aatcgacagg cgcaagtagt aaaacttacg
    10621 cgcaaatgca tcagtcagtt gtgaagccat gtacaccttt ccagatacgg gaggcgaagt
    10681 catttcttcc ttcgccctgg tggcaatctt tacgagcatt gtcacggcca aagcaccgta
    10741 tcagttgacc caggtgcaga ggctagagtg tttagtggtt aagccgatac tagcgtgtaa
    10801 atgagatttt taccatccac actttcgcta tgtaatcatg tatatagcgt catgatcgtg
    10861 ctatttcgct acagaataag ggaaaatggc ggttttactt tgatatacat catcagggag
    10921 gcatagcgtg catcgtcctt tagagataga agaaaatagt ttgtatcctc aatattggca
    10981 ggttaattgc tgtttccccg caatttgcgc tactgtagcg cggataatct gactccagga
    11041 aactatatgc gcaatcgtac gctggctgat cttgatcgtg tcgttgctct cggcggaggg
    11101 catggactgg gacgcgttct ctcatcactt tcgtctttgg gttctcgttt aacgggtatc
    11161 gtcaccacca cggataatgg tggctcgacg gggcgtattc gccgttcaga aggcggcatt
    11221 gcttggggcg atatgcgcaa ctgcctcaac cagctgataa cggaaccgaa cgtcgcctcc
    11281 gcgatgtttg aataccgttt tggcggcaat ggcgaacttt ccggtcataa tctcggaaac
    11341 ttgatgttaa aggcgctgga tcaccttagc gtgcggcctc tggaagccat caatttaatt
    11401 cgtaatctgc tgaaagtgga tacgcatttg attccaatgt cagagcatcc tgttgatctg
    11461 atggcgattg acgatcaggg gcatgaagtt tacggcgagg tcaatatcga ccagttaact
    11521 acgccgattc aagagttatt gttaacgcct aatgtacccg caacgcgtga ggcggttcac
    11581 gctatcaatg aagcggatct catcattatt gggcctggca gtttttatac cagcctgatg
    11641 ccaattctgc tgctgaagga aatcgcccag gcattacgcc gcacgccagc gccgatggtt
    11701 tatatcggca atctggggcg tgagttgagt ttacctgcgg ctaatttgaa gctggaaagc
    11761 aagctggcaa ttatggagca gtatgttggt aaaaaggtca ttgatgcggt catcgtcggg
    11821 ccaaaagtgg atgtctcggc ggtgaaagag cggattgtga tccaggaggt actggaggcc
    11881 agcgatattc cgtatcgtca tgaccgccag ttgttacata acgcgctgga aaaggcgtta
    11941 caggctttag gttaacggaa atccgaagga aaattccggc ttcctattga agacaaagtg
    12001 cgcgttgttt atgccggatg cggcgtgaac gccttatccg gcctacaaac cgcgcaaatt
    12061 caatatattg cggagaaaat gtaggcctga taagcgtagc gcatcagact gttttccgtt
    12121 tgtcatcagt cttcttcgct atcctgttac gatgcggcaa taaacaactc acgcaactga
    12181 tgcaactggt cacgaatttg tgccgcttct tcgaactcca gattctgcgc gtgttgcatc
    12241 atcagccctt ccagttcatg gattttctgc tgcaacgctt tcggcaacat atccatcggc
    12301 acattatccg gctcaacaat cgggcgcgat tttcctctgc ccttcgcttt ggttttggca
    12361 atgttctgcc ccagcgccag gatatcgacc actttcttgt tcaagccttg cggcgtaatg
    12421 ccgtgttcct cgttgtactt ctgctgtttc tcacggcgac gttcggtttc gccaatcgct
    12481 ttcgccattg atggagtgct cctatcgccg tagagaatcg ctttaccgtt aacgttacgt
    12541 gccgcacgac caatggtctg gatcaacgaa cgttcggaac gcaggaagcc ttctttgtca
    12601 gcgtcgagga tcgccaccag cgacacttcc ggcatatcca gaccttcacg cagtaagttg
    12661 atccctacca atacgtcgaa ctcacccaga cgcaagtcgc ggataatctc catacgctcg
    12721 acggtgtcga tatctgagtg aagataacgc acgcgctcac cgtgttcttc gagatattcg
    12781 gcaagatctt ccgccattcg cttggtcagt gtggtgacca gcacgcgttc gttaattgcc
    12841 gctcgctgac gaatctccga aagaagatca tcaacctgtg tcgccaccgg ccgcacttcg
    12901 ataatcgggt caagcaatcc ggttggacgc accacctgat ccaccacatc gccgccggat
    12961 ttttccagct cgtaattacc cggcgtcgcc gaaacataga tggtttgcgg cgctaatgct
    13021 tcgaactctt caaacttaag cggacggtta tccagcgctg atggcaggcg gaagccgtac
    13081 tccaccagtg tctctttacg cgcccggtca ccgcgataca tgccgccaat ttgtggaatg
    13141 gtgacgtgag attcatcgac gaccagcagc ccatcggcag gcaggtaatc aaacagcgtc
    13201 ggcggtggct cacccggtcc acgaccggag aggaagcgcg agtagttttc aatccccgaa
    13261 cagtagccca gctcgtccat catctccaga tcaaactggg tacgctgggt cagccgctgc
    13321 tcttccagca gtttgttgtt ttccaacagc actttgcgtc tggcggccag ttcttctttg
    13381 atctcctcca tcgcctgtac gatgcgctcg cgcggtgtga cgtagtgcgt tttcgggtag
    13441 atggtaaaac gtggaatagt ggaaacaatc tgcccggtca gcgggtcaaa taacgacaat
    13501 cgttccactt cctcgtcaaa cagttccacg cgaagtgcaa tgtcatccga ttctgccggg
    13561 aagatatcta tcacctcgcc acgaacgcgg aaagtaccac gctggaatgc ttgatcatta
    13621 cgagcgtatt gcagctccgc cagtcggcgc agaatcgcgc gctgatcgat aatcataccg
    13681 accgtgagat ggagcatcat cttgagatat aaatcaggat cgcccagacc ataaatcgcg
    13741 gaaacagacg ccactacaat cacatcacgc cgctccagca tcgctttggt ggcggacaaa
    13801 cgcatctgct caatatgttc gttaaccgag gcatctttct caatgaaggt gtcggaactc
    13861 ggtacatagg cttccggctg atagtagtcg tagtaggaga cgaaatactc caccgcgttt
    13921 tccgggaaga actctttcat ttcgccatac agctgggccg ccagcgtttt gttgggcgca
    13981 agtaccatgg ttgggcgctg aaggtcagca atgacattgg caatggtgaa ggttttcccg
    14041 gagccagtca cgccaagtaa cgtctggtgc gctaggccat cttccagccc ctcttcgaga
    14101 cgtcgaatcg cctctggctg atcgccagaa ggtttaaaag cggaattcag tttgaacggt
    14161 ttactcatga gtcgctacct gaaggagttg ggcgggcagg tatgtaattt tactcgtcgt
    14221 acttaattat gccaacaaat tatactggat aaaaaaacag ttcatcacca taatatttct
    14281 gatacagcgt aaactccgct gtcaatcatg agcaaaattt actctgtggc gagataaaac
    14341 tccagcctta ccgggttatc cccaaagcaa cggctttttt aacatttgtc aagatgagtg
    14401 atgacagttt tatggcaaga gatgcctgtt cagtgactgc cattgcattt atctaaccag
    14461 ttaaaaaata aaagatattt ctttgagccg tctttaacgc caattcgcgt gcaagccgcg
    14521 tattctctcg cttgcctcgt gttttctaac tctaatacac atggttatcc acaggaatag
    14581 tggataactg tctccagccc ctatcccccg ccgcctgggc aattcccaca ttcgccgcat
    14641 gaggcggaag taaaattttt ttcgtgatta ttttagaatt taattggtta aattgcagtc
    14701 aatcgaagac gcgatctcgc tcgcaattta accaaataca gaatggctac aacaaggcaa
    14761 ggtttatgta ctttccggtt gccgcatttt ctggattttc tgcaagccag gggatctctc
    14821 ccagcagcgg cgcgggaatc atgcgggtga gcgtggtcat atattcagcg tgacgttttc
    14881 ccggaggcgt aacatcgttc gccacccaac ccgccagagt cagtccggcg tgttgtattg
    14941 cctgcgcagt caacatcgcg tgattaatac agccgagttt cacaccaact accagtatca
    15001 ccggcagttg ttcctgtgtt acccaatctg caaaagtgaa agtgtcagaa agcggcgtaa
    15061 accagccgcc agcaccttcc actaacaccc agtcagcctg ttgttcaagc gcgcgtaatc
    15121 cggcgctcat taccaatgat tctatcggcc tgccctcttg ggcgctgatg atgtgcggcg
    15181 aggtgggttc tgcgaaggtg taaggattta ccgttgcgta atccagctgc aggctgctgt
    15241 tgtgctgtaa cgccagcgcg tcactattac gtaagccttc cggggtcttt tcgctgccag
    15301 aggcgaccgg tttataacct gccgtccgat aacctactgc cttcgcggct tgtaaaagtg
    15361 cacaactggc gacggttttc cccacttcgg tatccgttcc ggtgacaaaa taacgtttac
    15421 tcacgagcaa tcactcccaa aaaaagatga tacgtcagag gatatcgccc ctgttgttgc
    15481 ggccaggcca gttgcaatcg ctgcaactgc gaacgcgtta atattcgcgg gtcgcgccct
    15541 tcatgaagat gcgtagcacc gatgcctttc agcgaacgca tggcactgag cgcatcatca
    15601 aaccatagtg tgatgagctg aatatgatgt tgataacgca cgccgttcag cgactcttcg
    15661 atttcatctg gcggtaaaaa gcgattagca tgcggacgct cgtccaccgc ctgccgcgcc
    15721 tgatgcagtt cgggtaacga tccctgcacc agcgtggtaa acgcgaccac gcctccgggg
    15781 cgcaccaccc gatacagctc gcggagtgcc gtggataaat taccgcacca ctgcaccgcg
    15841 agattgctcc atgcaagatc gaacgtcgca gtcgctaacg gcagggattc gatatctcct
    15901 gccagataat gatctgcggc atctttatgg cgggcttgcg caagcattgg cggcgagaga
    15961 tctaacgccg tcacctgcgc gtgacgttcc cgccagtggc ggctcatcca gccaggtcca
    16021 caacccgcgt ccagtacgtg ggtgtattta cgctgtggaa gcattgccag taaggcgtca
    16081 gcactctggc gctgtagatc tgcatgttgc tcatagtgtg cggctgcccg accaaatgcc
    16141 gctgcaatgg cttgtttatt aaccgttgcc atgcagcacc tccagcagac ggtcgatatc
    16201 ctgcatttca tgcgcagcgg ttagcgttaa gcgcagtcgc gcagtaccag cgggtacggt
    16261 tggcgggcga atcgccgtga cccagcagcc ttgctggcgc agtttttctg ccagttgtaa
    16321 cgcacggctg ttatcaccga cgatcaatgg ctggatggcg ctgcatgaat cagccagcgt
    16381 aaacggcaaa tcctgtactc cggcacgaaa acgcgcaatg agtgacacca gtttttcgcg
    16441 ccgtgcatcc ccctcatcac ggcgaatgac cgccagcgac gcacgtaatg cctgcgcctg
    16501 agcgggcggc atactggtgc tgtagataag gtggcgggcg aattgcagca gataatccgc
    16561 caccgtactt gagcaaagca ccgcagcccc actgacgcca aatcctttgc caaaagtcac
    16621 taccagcaat tctggtttta ccttttgcag ccagcagctg ccgcgcccct gctccccgat
    16681 aacgcccgtg ccgtgggcat catcgaccat caaccagcca ttgtgctgtt gcgttacctg
    16741 ctggatttcc gccagtggcg cgctatcgcc gtccatgctg aacacgcctt ctgtcaccac
    16801 cagttgctgc cccggacagg gggaagcaag cagtcgcgcc agatgagcga catcgttatg
    16861 agtaaaacgg cgaagctgcg acgggcttaa actggcagcc tccagcaatg aggcatggct
    16921 aagccggtcg gcaacaatac ggtcctcttt cgccatcatc gcggcaatga ctgcctgatt
    16981 cgcggcaaaa ccagagataa acagcagcgc ccgcgaatag ccaagccact cggccagttc
    17041 ttcttccagc gcctgatgca ccacgctata accgctgacg tgaccggagc cgccgctacc
    17101 gatgccaaat tgctccgccc cctgcttcca ggcacggata atttgcggat gatggcttaa
    17161 acctaaataa tcgttactgg aaaagttcag atactggcga tcatccgcca ccagccagcg
    17221 tccggctcct tgcgccaccg gataacggcg acgcagggca tcggcagcac gccgcgcatc
    17281 gagcgccgcg ttgattttct cctgccagct cataatgctg ccgcgttgta atattcgtcg
    17341 gtgtccgggg tcatcagcgc ctgtccaaga cgttgctgtt gttcgttatc ccctgccagc
    17401 acggcagttt gctgcggatt tagccccagt ttgcggaaca gttgcaggtc tttatcttct
    17461 tccggattcg gcgtggtcag cagtttgcaa ccgtagaaaa tcgagtttgc gcctgccata
    17521 aagcacatcg cctgagtctg ttcgttcatc tgctcgcgtc cggcagaaag gcgcacgtaa
    17581 gaggtcggca tcatgatccg cgctacggca atggtgcgaa taaaatcaaa ggcatcaacg
    17641 tcatcgttat cggcaagcgg cgtgcctttc accttcacca gcatgttgat tggtacgctt
    17701 tccggcggcg tcggcaggtt tgccagttgc agcaataatc cggcgcgatc ttttaccgtt
    17761 tcgcctaagc ccacaatgcc acctgagcag actttgatcc cggcatcgcg cactttttcc
    17821 agcgtatcga ggcgttcctg ataagtgcgt gtggtgatga tattgccgta aaactccggc
    17881 gaggtgtcca ggttgtggtt gtagtaatcc agcccggcgt tcgcgaggcg ctgcgcctga
    17941 gattcactca acgtgcccag cgtcatacac gcctccagcc ccatcgcttt taccccctgc
    18001 accatttgtt ccaggtacgg catatcgcgt tcgtggggat tcttccatgc cgcgcccata
    18061 cagaagcgcg tcgatcctgc cgctttcgct ttgcgcgccg actccagcac ctgttcaact
    18121 tccatcaacc gctcggcttc cagcccggtt ttgtagcgcg agctttgcgg gcagtatttg
    18181 caatcttccg gacaagctcc ggtcttaatc gacagcaacg tgctgacctg cacctgacga
    18241 ggttcgaaat gctggcgatg cacctgctgc gcttcaaaca gcagatccag caacggtttt
    18301 tcaaataatt ctgtgacttg cgacaatgtc cagcgtgggc ggtgagccat ggggcttctc
    18361 caaaacgtgt tttttgttgt taattcggtg taggcttgta aacctaaatc ttttcaattt
    18421 ggtttacaag tcgattatga caacggacga tcttgccttt gaccaacgcc atatctggca
    18481 cccatacaca tccatgacct cccctctgcc ggtttatccg gtggcgagcg ccgaaggttg
    18541 cgagctaatt ttgtctgacg gcagacgcct ggttgacggt atgtcgtcct ggtgggcggc
    18601 gatccacggc tacaatcacc cgcagcttaa tgcggcgatg aagacgcaaa ttgatgccat
    18661 gtcgcatgtg atgtttggcg gtatcaccca tgcgcccgcc atcgagctat gccgcaaact
    18721 ggtggcaatg acgccgcaac cgctggagtg cgtttttctc gcggactccg gttccgtagc
    18781 ggtggaagtg gcgatgaaaa tggcgttgca gtactggcaa gccaaaggcg aagcgcgcca
    18841 gcgttttctg accttccgca atggttatca tggcgatacc tttggcgcga tgtcggtgtg
    18901 cgatccggac aactcaatgc acagcctgtg gaaaggctat ctgccagaaa acttgtttgc
    18961 tcccgccccg caaagccgca tggatggtga atgggatgaa cgcgatatgg ttggttttgc
    19021 ccgcctgatg gcggcgcatc gtcatgaaat cgcagcggtg atcattgagc cgattgtcca
    19081 gggcgcaggc gggatgcgca tgtaccatcc ggaatggtta aaacgaatcc gcaaaatgtg
    19141 cgatcgcgaa ggtatcttgc tgattgccga cgagatcgcc accggatttg gtcgtaccgg
    19201 caaactgttt gcctgtgaat atgcagaaat cgcgccggac attttgtgcc tcggtaaagc
    19261 cttaaccggc ggcacaatga ccctttccgc cacacttacc acgcgcgagg ttgcagaaac
    19321 catcagtaac ggcgaagccg gctgctttat gcatgggcca acttttatgg gcaatccgct
    19381 ggcctgcgcg gcagcaaacg ccagcctagc gattatcgaa tccggcgaat ggcagcagca
    19441 ggtggcggct attgaagtgc agctgcgcga gcaactggca ccagcccgtg atgccgaaat
    19501 ggttgccgat gtgcgcgtac tgggggcaat cggtgtggtc gaaaccactc gtccggtgaa
    19561 tatggcggcg ctgcaaaaat tctttgtcga acagggggta tggatccggc cttttggcaa
    19621 actgatttac ctgatgccgc cctatattat tctcccgcaa cagttgcagc gtctgaccgc
    19681 agcggttaac cgcgcggtac aggatgaaac atttttttgc caataacggg aagtcagcgt
    19741 gagggtttct ggctacactt tctgcaaaca agaaaggagg gttcatgaaa ctcatcagta
    19801 acgatctgcg cgatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg
    19861 atggcgataa tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt
    19921 ttgttgtcac ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag
    19981 ttgttaattt acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag
    20041 caatgccaga cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg
    20101 cagcgccgcc gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag
    20161 agcgtattga tgtcgatgaa ggagccagcg gcgcgatggt cgggtttaac gttcatttcc
    20221 actctctggc aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc
    20281 aatgccatct ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg
    20341 tttcgctcaa gttagtataa aaaagctgac cgagaaacag taaaaatcat taatatcaat
    20401 gcattataat acatttagct taaaaaatag actgcataat actgcaaaac acaacatatc
    20461 cagtcactat gaatcaacca cttagatggt attagtgacc tgatacagag cattagcgca
    20521 aggtgatttt ttgtcctctt gcgctaattt tttgtcaacg cactgggtta gctgaattta
    20581 caaccatgct cagtatctcg ataagcgcag agaaatgatg ctgtggtggg cagactactg
    20641 gcttgatgaa aaggtagggc gaccaaccgc gttttttcag ttcagtcagc acattgagcc
    20701 agaacttcac cccttcattt tcggccagcc acatccccaa cagttcttcc tgttcttaac
    20761 tatttacagg cgcgtttttt taaagaatcc tcactccatt tgcacgaata tattaatata
    20821 taaacaatga ggatcaataa taaatgaaac ctgcccacaa tccttctttt ttccgctcct
    20881 tttgtggttt aggatgtata tcccgtttat ccgtagaaga gcaaaatatc acggattatc
    20941 accgcatctg ggataactgg gccaaggaag gtgctgcaac agaagaccga acacaggcag
    21001 ttcgattact gaaaatatgt ctggcttttc aagagccagc cctcaattta agtttactca
    21061 gattacgctc tctcccatac ctgcccccgc acatacaaga acttaacatc tctagcaatg
    21121 agctacgctc tctgccagaa ctccctccgt ccttaactgt acttaaagcc agcgataaca
    21181 gactgagcag gctcccggct cttccgcctc acctggtcgc tcttgatgtt tcacttaaca
    21241 gagttttaac atgtttgcct tctcttccat cttccttgca gtcactctca gcccttctca
    21301 atagcctgga gacgctacct gatcttcccc cggctctaca aaaactttct gttggcaaca
    21361 accagcttac tgccttacca gaattaccat gtgaactaca ggaactaagt gcttttgata
    21421 acagattaca agagctaccg ccccttcctc aaaatctgag gcttttaaac gttggggaaa
    21481 accaactaca cagactgccc gaacttccac aacgtctgca atcactatat atccctaaca
    21541 atcagctgaa cacattgcca gacagtatca tgaatctgca catttatgca gatgttaata
    21601 tttataacaa tccattgtcg actcgcactc tgcaagccct gcaaagatta acctcttc
//
LOCUS       DAJTUN010000064        21508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTUN010000064 DAJTUN010000000
VERSION     DAJTUN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.064"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO470_002260"
     CDS             complement(40..2109)
                     /gene="glyS"
                     /locus_tag="OO470_002260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6251490.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO470_002261"
     CDS             complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="OO470_002261"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6251491.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3125..3424)
                     /locus_tag="OO470_002262"
     CDS             complement(3125..3424)
                     /locus_tag="OO470_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6251492.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3599..4594
                     /gene="wecH"
                     /locus_tag="OO470_002263"
     CDS             3599..4594
                     /gene="wecH"
                     /locus_tag="OO470_002263"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6251493.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4636..5073)
                     /locus_tag="OO470_002264"
     CDS             complement(4636..5073)
                     /locus_tag="OO470_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6251494.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5119..5460)
                     /locus_tag="OO470_002265"
     CDS             complement(5119..5460)
                     /locus_tag="OO470_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251495.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO470_002266"
     CDS             complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="OO470_002266"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6251496.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO470_002267"
                     /pseudo
     CDS             complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="OO470_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8843..9835
                     /gene="xylF"
                     /locus_tag="OO470_002268"
     CDS             8843..9835
                     /gene="xylF"
                     /locus_tag="OO470_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6251497.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9913..11454
                     /gene="xylG"
                     /locus_tag="OO470_002269"
     CDS             9913..11454
                     /gene="xylG"
                     /locus_tag="OO470_002269"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6251498.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11432..12613
                     /gene="xylH"
                     /locus_tag="OO470_002270"
     CDS             11432..12613
                     /gene="xylH"
                     /locus_tag="OO470_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6251499.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12691..13868
                     /gene="xylR"
                     /locus_tag="OO470_002271"
                     /pseudo
     CDS             12691..13868
                     /gene="xylR"
                     /locus_tag="OO470_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13976..14800)
                     /locus_tag="OO470_002272"
     CDS             complement(13976..14800)
                     /locus_tag="OO470_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6251500.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTVSQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15120..17150
                     /gene="malS"
                     /locus_tag="OO470_002273"
     CDS             15120..17150
                     /gene="malS"
                     /locus_tag="OO470_002273"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6251501.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17328..18581
                     /gene="avtA"
                     /locus_tag="OO470_002274"
     CDS             17328..18581
                     /gene="avtA"
                     /locus_tag="OO470_002274"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6251502.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18732..19205)
                     /locus_tag="OO470_002275"
     CDS             complement(18732..19205)
                     /locus_tag="OO470_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6251503.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19307..20155)
                     /gene="yiaJ"
                     /locus_tag="OO470_002276"
     CDS             complement(19307..20155)
                     /gene="yiaJ"
                     /locus_tag="OO470_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6251504.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20356..21354
                     /gene="yiaK"
                     /locus_tag="OO470_002277"
     CDS             20356..21354
                     /gene="yiaK"
                     /locus_tag="OO470_002277"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6251505.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21366..>21508
                     /locus_tag="OO470_002278"
     CDS             21366..>21508
                     /locus_tag="OO470_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6251506.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5403 a         5488 c         5282 g         5335 t
ORIGIN      
        1 aaagcctgcc agatggcagg ctttttaata acggcgttat tactgcaaca gcgaaatatc
       61 cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg gtcagacggt tgagacgcaa
      121 ttctttgtca tcaaccatga ccatcacttt atcgaagaaa gcatcaaccg gctcacgcag
      181 ctctgccagt tcgaccagcg catcctggta acgaccttcc gcaaagtacg gctccagctt
      241 gtcacgtagc accacaacct gcatcgccag tttaatttct tccggctctt tcagggtaga
      301 ggcattcacg cgatcgctca gcacttcgtc agatttcgcc aggatgttag atacacgttt
      361 gttcgccgcc gccagtgctg cagctgcttc cagggtacgg aagtgcgata ccgctttcat
      421 gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc agtaccgcct ggatggtgtc
      481 aacggtataa ccttcgtcct gataccaggc gcggaagcga ccgagcataa agtcgataac
      541 atcatcaact acgttggcat tagtcagctt atcgccatac agacgcaccg cttcttcggt
      601 cagcgtttgc agatcaaggt tgaggttctt ctcaacgata attcgcagca cgccaagtgc
      661 ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga tgctgaccga taccgaagat
      721 acccgccaga gtatccatct tatcagcaat cgccagcgca caagctaccg ggttggacgg
      781 caggtcatca ccggcaaagc gcggctgata ctgctcattc agcgcgacgg ctacgtcttc
      841 cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg ccttgggtgt cggtgaactc
      901 gaagaccatg ttggtcatca ggtcacactt agacagcagg cccgcacggg ttgcgtggtt
      961 aacgtcagcg ccaatctgtt cggcgatcca gccagccagc gcctggatgc ggtcagtctt
     1021 gtcacgcagc gtacccagct gttgctggaa cagcacggtt tgcaggcgcg gcaggttatc
     1081 ttccagacgt tttttgcggt cggtgttgaa gaagaactcg gcatccgcca gacgtggacg
     1141 aacgactttc tcgttaccag agataatttg ctgcggatct ttcgattcga tgttggcaac
     1201 gaagataaag ttcggcagca gtttgccgtc gttcgcatac accgggaagt acttctggtc
     1261 acctttcatg gtgtaaacca gggcttcagc cggcaccgcg aggaatttct cttcgaattt
     1321 tgcggtcaga acgaccggcc actccaccag cgaagccact tcttccagca ggctttcgct
     1381 taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg gcatcggctt taatcttcgc
     1441 tttacgttct tcgtaatcgg cgatgacttt cccgcgctca cgcagaattt ccggatactg
     1501 atcggcgttg tcgatggtga attccggttc gcccataaag cggtggccgc gaatcacgcg
     1561 atcggactga atgcccagaa tggttgccgg aatgactttg tcgcccagca gcagggtcac
     1621 ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc cagcgcatca gtttcgggat
     1681 cggcagtttc gccagcgaag tcgcaaccat attcggcagc agtgcttcgg tgctttcgcc
     1741 cttcacatgg gcgcgataca gcagccattc gcctttatcg gtagtcagac gctcagcctg
     1801 gtcaacggta ataccacaac cacgcgccca accttctgcc gctttgctcg gttttccttc
     1861 agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg atttcacgat ctggttgtgc
     1921 ttccgccagg ttagctactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt
     1981 gccgtgtgcg aggcctgcgt tatccagctc cgcagtaaag ttcgcagcaa aggactctgc
     2041 caggctgcgc agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt
     2101 ctcagacata gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg
     2161 ggaagcgtaa tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatgtagcg
     2221 ctgacgctcg gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc
     2281 tttcagaata cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagctgctg
     2341 cgcttctttc tcgtactgct caaagcaggt gaacaggaag tccacatccg cgtattcgaa
     2401 gttgtaagtg gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc
     2461 cagcgggccg tcgctccaga ctaggtcgta aacgctgtct acgccctgaa tgtacatggc
     2521 cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt ttacactcca gaccaccaac
     2581 ctgctggaag taagtgaact gcgtcacttc catgccgttt agccacactt cccagcccag
     2641 tccccaggcc cccagagtcg ggttttccca gttatcttcc acgaaacgga tgtcgtgaat
     2701 agtcgggtcc atgcccagct ctttcagaga accgaggtac agctcctgaa tattgtccgg
     2761 cgatggctta atgaccacct ggaactgata gtagtgctgt aaacggttgg ggttttcgcc
     2821 gtagcgacca tcggtcgggc gacgagaagg ctgaacataa gcagccgcca tcggttctgg
     2881 ccccagcgcg cgcagacagg tcattgggtg agaggttccc gcgccgactt ccatgtccaa
     2941 tggttgaaca atggtgcagc cctggcgagc ccagtaatcc tgtaaggtca ggatcaagcc
     3001 ctggaaggtc ctggtatcaa acttttgcat attatttcgt gctggatacg tgtggattta
     3061 aaggaaggga tcagtatacc cgctggatgg aagatataca gtacgaaacg ggaaaaagca
     3121 gggcttaacg catggaaaga tgcaaaaaat ggccatctgc gtcaaaagaa cagacaaact
     3181 gctctttacg gaaggtataa ccgcgcattt catagcttcc ctggaactgt tcgaaggcgg
     3241 tgacgtcgat tttttgctcg ccggtgttat aacgttttgc cgcctgatcc ttgcataacg
     3301 cttccatatt caacgaacgc agaggatcaa cctttacccg ttgtgctttc tgcacgggtg
     3361 acggtgtaga acaacccact agcaccataa gcgccattgc cggaaagaaa gcgttcatca
     3421 tcattaaatt accgtctggt ttgcaggtga gtcttattat ttatatcggt atagtggctg
     3481 atatcttacg aatttcctta agcctctata ggctgtagtg ataaaactca gattattcca
     3541 gtgcgccaac caaatgattt atcggtcaga ctttttttaa ccagatacag agacacagat
     3601 gcagcccaaa atttactgga ttgataacct gcgagggata gcgtgtttaa tggtggtgat
     3661 gattcacacc actacctggt atgtgaccaa tgctcatagt gttagccccg tcacatggga
     3721 tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg ccgctatttt tcatgatttc
     3781 cggctatctc ttttttggcg aacgcagcgc ccagccgcgc catttcttgc gtatcggctt
     3841 atgtctgatt ttttatagcg caatcgcact gctctacatt gcgctgttta cctccatcaa
     3901 tatggagtta gcgctgaaaa acctgctgca aaagccagtg ttttaccact tgtggttttt
     3961 cttcgcgatt gcggtgattt atctggtttc accgctgatt caggtgaaga acgtcggcgg
     4021 aaaaatgttg ctggtgctaa tggtggtgat tggcattatc gctaacccaa acacagtgcc
     4081 gcagaaaatt gacggttttg aatggctgcc aattaactta tatatcaatg gcgatacttt
     4141 ttactacatt ctgtatggca tgttgggccg cgctataggg atgatggaca cacagcataa
     4201 agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg gtttttatta tctctcgcgg
     4261 gacattatat gaattgcagt ggcgcggaaa ttttgccgat acctggtatc tttactgtgg
     4321 gccgatggtt tttatctgcg caatcgcgct attgactctg gttaaaaaca cgctggatac
     4381 gcgtaccatt cgcggacttg gcttaatctc ccgccattca ttgggtatat acggattcca
     4441 cgccttgatt atccatgcgc tgcgcacccg gggaattgag cttaaaaatt ggccaatact
     4501 ggatattatt tggatctttt gcgcgacgtt ggcagcgagt ttgttacttt ctatgctggt
     4561 acaacgaatc gacagaaaca gattagtgag ttaagtaaaa gcccgttcac attggactga
     4621 ccgggcttac gtgaattatt cgctgtattc ttcctgggta acctgtgttt cttttggcgg
     4681 gtttattccg gcatcacgaa tattcttctg caccgctact gcaccaaaca agcttaagaa
     4741 gaaagccagt ccataaaaac ctttttcact gagtaataat gtcgcattcc acagacctac
     4801 catcagtaga gcaacagaga taataaagac agtcaggcag gtcatataat aaatggaagt
     4861 ggtcggtatg ccttcatact tgtcccgaac ggtcttttga taagacgcag cagaaaacag
     4921 tcctaacacc agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca
     4981 tagccctaac aggtaggtaa cgataccacc aacgagagct atccatgaca caatactaaa
     5041 ggccggtgaa taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt
     5101 gtaagagctt tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac
     5161 aaaaaaggcg atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc
     5221 gataaacaag agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt
     5281 atccagttgc ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat
     5341 taacacgcca agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac
     5401 cagataaacc agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat
     5461 aacgatctcc atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa
     5521 aatgccatta acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag
     5581 atatatagat gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa
     5641 tggcagaagt tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata
     5701 acgctgcgca tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag
     5761 cgatttctct ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac
     5821 atcccccccc gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca
     5881 gtactcacta cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc
     5941 atgcacgaca tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc
     6001 cagttcattg gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt
     6061 attgtgtggt gtacgctcgc cggaaagata aggcagaaac caaactggct cggcactttc
     6121 atcagcctgt tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc
     6181 ggcccaatcc agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg
     6241 tagcgcatgg caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac
     6301 agcaaaatag acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc
     6361 cacaccaact gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat
     6421 accccacgct ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa
     6481 tgcgggcatc tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg
     6541 ctttgcgaca tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc
     6601 cgtcatacgc agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat
     6661 ctccggctca tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag
     6721 gttgccggta atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc
     6781 acagcgcccg tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa
     6841 ggttgctcca tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg
     6901 atcgcccaga gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc
     6961 cgaccaaagt ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaag caaccacctc
     7021 accctgctcg ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata
     7081 catatcgatc gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac
     7141 tgcacagtta gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc
     7201 tggcgaccac tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct
     7261 gccagtgaca tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa
     7321 cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc
     7381 gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta
     7441 ctttgacgac gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg
     7501 agaatttcat acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc
     7561 cagcccagtt gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg
     7621 gcggtggcta tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg
     7681 ttcagtttaa tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca
     7741 tcgtaatcat attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc
     7801 tagaagccga ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca
     7861 cgctcctgac gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg
     7921 acatagtttt caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc
     7981 gcccagctga agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt
     8041 gtaaagcagt tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc
     8101 aggacatcaa ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg
     8161 gaaacatcca catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat
     8221 gcgacatctg ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga
     8281 ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag
     8341 gcggcaaaac gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag
     8401 tgacggaatg ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg
     8461 tcaaaatagg cttgcatatt gaactccata atcaggtaat gccgcgggtg atggatgatg
     8521 tcgtaatatt gggcactccc tttcaattgc tcaattatgt tatttcacac tgctattgag
     8581 ataattcaca agtgtgcgct cgctcgcaaa ataaaatggg atgatgaaac tgggtaattc
     8641 ctcgaagata aaaatgcaat aagtacaatt gcgcaacaaa agtaagatct cggtcataaa
     8701 tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt
     8761 ttagttgcta aaaattggtt acgtttatcg cggtgattgt tacttattaa agctgtcctc
     8821 taactacaga aggccctaca ccatgaaaat aaagaacatt ctactcaccc tttgcacctc
     8881 acttctgctt accaacgttg cggcacacgc caaagaagtc aaaataggta tggcgattga
     8941 tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc
     9001 tctcggtgcg aaagtatttg tacagtctgc aaatggcaat gaagaaacac aaatgtcgca
     9061 gattgaaaac atgattaacc ggggtgtcga tgttcttgtc attattccgt ataacggtca
     9121 ggtattaagt aacgttgtaa aaaaagccaa acaagaaggt attaaagtat tagcttacga
     9181 ccgtatgatt aacgatgcgg atatcgattt ttatatttct ttcgataacg aaaaagtcgg
     9241 tgaactgcag gcaaaagcct tggtcgatat tgttccgcaa ggtaattact tcctgatggg
     9301 cggctcgccg gtagataaca acgccaagct gttccgcgcc ggacaaatga aagtgttaaa
     9361 accttacgtt gattctggaa aaattaaagt cgttggtgac caatgggttg atggctggtt
     9421 accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc gccaataata acaaaattga
     9481 tgctgtagtt gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca
     9541 aggtttatca gggaaagtag caatttccgg ccaggatgcg gatctcgcag gtattaaacg
     9601 tattgctgcc ggtacgcaaa ctatgacggt gtataaacct attacattgc tggcaaatac
     9661 tgccgcagaa attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact
     9721 gaataatggc ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa
     9781 caacatcaaa gatacggtaa ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt
     9841 acgccccggc gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta
     9901 ggtggagtcg ttatgtctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt
     9961 gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt
    10021 ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat
    10081 ggctcctacg aaggcgaaat tatttttgcg ggagaagaga ttcaggcgag tcacatccgc
    10141 gataccgaac gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg
    10201 accgtgctgg aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat
    10261 gacctgatga cgctacgcta tcagaagctg ctcgcacagg tcagtttatc catttcacct
    10321 gatacccgcg ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca
    10381 cttaataaac aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag
    10441 gaaacgtcgg ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt
    10501 tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc
    10561 gacggacagc acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc
    10621 atgatggtcg ggcgagagtt aaccgcgatt taccctaatg aaccacatac caccggagat
    10681 gaaatattac gtattgaaca tctgacggca tggcatccgg tcaatcgtca tattaaacga
    10741 gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt
    10801 ggtgccggac gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa
    10861 ggaaaaattt atattgatgg caaacaggta gatattcgta actgtcagca agccatcgcc
    10921 caggggattg cgatggtacc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg
    10981 gttggtaaaa atattaccct cgccgcactc aataaattta ccggtggcat tagccagctt
    11041 gatgacgcgg cagagcaaaa atgtattctg gaatcaattc agcaactcaa agttaaaacg
    11101 tcgtcccccg accttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc
    11161 gcccgctgtc tgttacttaa cccgcgcatc ctcattcttg atgaacctac caggggtatt
    11221 gatattggcg cgaaatatga gatctacaaa ttaattaacc aactcgtcca gcagggtatt
    11281 gccgttattg tcatctcttc cgaattacct gaagtgctcg gccttagcga tcgtgtactg
    11341 gtgatgcatg aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag
    11401 gtgatggaag ccgcattgag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa
    11461 attggccgta ccgacatccg gtggcttttc tgggctgaaa tcactgaatt tgcaggtctt
    11521 cgtgatgatt gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc
    11581 ctacttaagc gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct
    11641 cgcggtagga atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat
    11701 gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc
    11761 acttaccatc attgtgacgt tggttctggg actgcttctc ggtgcctgga acggatggtg
    11821 ggtcgcgtac cgcaaagtcc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg
    11881 cggcatactc attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc
    11941 acaaattggg caaagctatc tccccgccag taccggcttc atcattggcg cgcttggctt
    12001 aatggctttt gttggttggc aatggcgcgg aagaatgcgc cgtcaggctt tgggtttaca
    12061 gtctccggcc tctaccgcag tagtcggtcg ccaagcttta accgctatca tcgtattagg
    12121 cgcaatctgg ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt
    12181 gctgttactc ggcggaatgt ttatggcaac gcggacggca tttggacgac gcatttatgc
    12241 catcggcggc aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact
    12301 tgccgtgttc gcgattaacg gattaatggt agccatcgcc ggattaatcc ttagttctcg
    12361 acttggcgct ggttcacctt ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg
    12421 cgtgattggc ggcaccagcc tggctggcgg tgtgggaagc gttgccggag cagtaatggg
    12481 ggcatttatc atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg
    12541 gcagtatatc gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa
    12601 acgccgttct tgattttgat aaaaattttc tcaaagccgg ttacgtatta ccggttttga
    12661 gtttttgcat gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta
    12721 ctgttcaatg ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta
    12781 caggcatcac aatcagaatg ggatattttc attgaagaag atttccgcgc ccgcattgat
    12841 aaaatcaagg actggttagg agatgtcgtc attgccgact tcgacgacaa acagatcgag
    12901 caagcgctgg ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca
    12961 gaaagttacc cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca
    13021 tttttgcatt taaagagaaa ggcgttaacc gctttgcttt ttatggtctt ccggaatcaa
    13081 gcggcaaacg ttgggccact gaacgcgaat atgcatttcg tcagcttgtc gccgaagaaa
    13141 agtatcgcgg agtggtttat caggggttag aaaccgcgcc agagaactgg caacacgcgc
    13201 aaaatcggct ggcagactga ctacaaacgc tgccaccgca aaccgggatt attgccgtta
    13261 ctgacgcccg agcgcggcat attctgcaag tatgtgaaca tatacatatt cccgtaccgg
    13321 aaaaattatg cgtgattggc atcgataacg aagaactgac ccgctatctg tcgcgtgtcg
    13381 ccctttcttc ggtcgctcag ggcgcgcggc aaatgggcta tcaggcggca aaactgttgc
    13441 atcgattatt agataaagaa gaaatgccgc tacagcgaat tttggtccca ccagttcgcg
    13501 tcattgaacg gcgctcaaca gattatcgct cgctgaccga tcccgccgtt attcaggcca
    13561 tgcattacat tcgtaatcac gcctgtaaag ggattaaagt ggatcaggta ctggatgcgg
    13621 tcgggatctc gcgctccaat cttgagaagc gttttaaaga agaggtgggt gaaaccatcc
    13681 atgcaatgat tcatgccgag aagctggaga aagcgcgcag tctgctgatt tcaaccacct
    13741 tgtcgatcaa tgagatatcg caaatgtgcg gttatccatc gctgcaatat ttctactctg
    13801 tttttaaaaa agcatatgac acaacgccaa aagagtatcg cgatgtaaat agcgaggtca
    13861 tgttgtagcg atgatgagaa ttgtcggcgt cacatcaggg aactcagcac agccacctga
    13921 tgcgagcaaa agccttatcc gaccgacaaa tcagataagg ctcgaggaaa tgaaattaca
    13981 tatgcgccgc gattaaccgt tggttatcct ggtacattgc gaacaggtag ttgttataac
    14041 tcttcccttt ggtcgagtag cccttcagct tgtgaatcat cgctgtggca gtcacttcct
    14101 gatccgcttt acgcagctgt gcacgcgatt tacggaacga agagtaagcc gggtgcgtat
    14161 tcaggttagt gacataggcg ctcaccgatt ctttgacaga actaaactgt gagtaccctt
    14221 tcactttacc cggcgcattg gtacaacgtc ctttcatgca tttcatgccg aacagattgt
    14281 tattgttgcg cgccagcttc gacgttcccc aaccgctttc tgctgcagcc atcgtcgcca
    14341 ccatactggt agggataatg tctacgcgtt caagcaaggt attccacggg atttttcgcg
    14401 tattaccaga ccacttcacc ttgtagcgtt tggcgatgtc tttcagacgc gcacgctcag
    14461 caggtgacca ttggccctga tactgttttg aaatcagcca gttacgttct gcagtaatgg
    14521 ctgcattttg gctggtaatg taaggcatta cggtccggag aaacgccttt ttccttggtg
    14581 ttccggaagg gtattttcgc aaatcaggaa gtgaactact ctttgcacta ttgcgagaat
    14641 actcttgttt actgcttacc tgtttattac tagctttagt taagtgggac ttttgactca
    14701 ctgttgttgt gtgcgtcttc gctaacacct cactcgaaaa caccagagtg agtaacataa
    14761 gaatcatcgc cccatatcgt cgtatgggag tcaaaatcat caggtctcct ggtcggattt
    14821 aatcattcca acaccttata tttttcacaa atttgagagt tgaatctcaa atcatatcaa
    14881 aaatagctgt caagagcacc ccaaggaata gtccaaatct gaaactatgt cacgagttaa
    14941 cgattcagat tggcgctaaa tcgcagaaaa tgtgggggtt atcgcaaaat tcagccgttt
    15001 tttgcgcgcg atcgctcacc cttgcttctc atcctgtgga cttaccgctc agggatgagt
    15061 tttgtctggc ttatcgctgg caaactgtct gaaatcgcag caataaggac tcatccacca
    15121 tgaaactcgc cgcctgtttt ctgacactcc ttcctggctt cgccgttgcc gccagctgga
    15181 cttctccggg gttccctgcc tttagcgaac agggaacggg aacatttgtc agccacgcgc
    15241 agttgcccaa aggtacgcgt ccactcacgc taaattttga ccaacagtgc tggcagcctg
    15301 cagatgcgat aaaactcaat cagatgcttt ccctgcaacc ttgtagcaac acgccgcctc
    15361 aatggcgatt gttcagggac ggcaaatata cgctgcaaat agacacccgc tccggtacgc
    15421 caacattgat gatttccatc cagaacgccg ccgaaccggt agcaaacctg gtccgtgaat
    15481 gcccgaaatg ggatggatta ccgctcacgc tggatgtcag cgccactttc ccggaaggag
    15541 ccgccgtacg ggattattac agccaacaaa ttgcgatagt gaagaacggt caaataacgt
    15601 tacaacccgc tgctaccagc aacggtttac tcctgctgga acgggcagaa actgacacat
    15661 ccgccccttt cgactggcat aacgccacgg tttactttgt gctgacagat cgtttcgaaa
    15721 acggcgatcc cagtaatgac cagagttacg gacgtcataa agacggtatg gcggaaattg
    15781 gcacttttca cggcggcgat ttacgcggcc tgaccaacaa actggattac ctccagcagt
    15841 tgggcgttaa tgctttatgg ataagcgccc catttgagca aattcacggc tgggtcggcg
    15901 gcggtacaaa aggcgatttc ccgcattatg cctaccacgg ttattacaca caggactgga
    15961 cgaatcttga tgccaatatg ggcaacgaag ccgatctacg gacgctggtt gatagcgcac
    16021 atcagcgcgg tattcgtatt ctctttgatg tcgtgatgaa ccacaccagc tatgccacgc
    16081 tggcggatat gcaggagtat cagtttggcg cgttatatct ttctggtgac gaagtgaaaa
    16141 aaacgctggg tgaacgctgg agcgactgga aacctgccgc cgggcaaacc tggcatagct
    16201 ttaacgatta cattaatttc agcgacaaaa caggctggga taaatggtgg ggaaaaaact
    16261 ggatcagaac ggatatcggc gattacgaca atcctggatt cgacgatctc actatgtcgc
    16321 tggccttttt gccggatatc aaaaccgaat caactaccgc ttctggtctg ccggtgttct
    16381 ataaaaacaa aacggatact cacgctaaag tcatcgaagg ctttacgcct cgcgattact
    16441 taacccactg gttaagtcag tgggtccgcg actatgggat tgatggtttt cgggtcgata
    16501 ccgccaaaca tgttgagttg cccgcctggc agcaactgaa aaccgaagcc agctccgcgc
    16561 ttcgcgaatg gaaaaaaact aaccccgaca aagcattaga tgacaaacct ttctggatga
    16621 ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta ctatcgccac ggcttcgatg
    16681 cgatgatcaa tttcgattat caggagcagg cggcgaaagc agtcgattgt ctggcgcaga
    16741 tggatacgac ctggcagcaa atggcggaga aattgcaggg tttcaacgtg ttgagctacc
    16801 tctcgtcgca tgatacccgt ctgttccgtg aagggggcga caaagcagca gagttattac
    16861 tattagcgcc aggcgcggta caaatctttt atggcgatga atcctcgcgt ccgttcggtc
    16921 ctacaggttc tgatccgctg caaggtacac gttcggatat gaactggcag gatgttagcg
    16981 gtaaatctgc cgccaacgtc gcgcactggc agaaaatcag ccagttccgc gcccgccatc
    17041 ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa gcagggctac ggctttgttc
    17101 gtgagcatgg cgacgataaa gtgctggtca tctgggctgg gcaacagtga cttttccggc
    17161 ttcccgttcg tcagtacctc gggaagccgc caaccaggat aaaatgtcag ccctaatcag
    17221 cgttgcggga taaagcaccg ctcactcttc aacagaccga tttgcacccc agcaaatgta
    17281 gcgttattgt taccttcctt gctacagagt tcgacagata tcccgctatg acattctccc
    17341 tttttggtga caaatttacc cgccactccg gcattacgct gctgatggaa gatctgaacg
    17401 acggtttacg cacgcctggc gcgattatgc tcggcggcgg taatccggcg cagatcccgg
    17461 aaatgcagga ctacttccag acgctactga ccgacatgct ggaaagtggc aaagcgactg
    17521 atgcactgtg taactacgac ggtccacagg ggaaaacgga gctactcaca ctgcttgccg
    17581 gaatgctgcg cgagaagttg ggttgggata tcgaaccaca gaatattgca ctaacaaacg
    17641 gcagccagag cgcgtttttc tacttattta acctgtttgc cggacgccgt gccgatggtc
    17701 gggtcaaaaa agtgctgttc ccgctggcac cggaatacat tggctacgct gacgccggac
    17761 tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga actgctgccg gaaggccagt
    17821 ttaaatacca cgtcgatttt gagcatctgc atattggcga agaaaccggg atgatttgcg
    17881 tctcccggcc gacgaatcca acaggcaatg tgattactga cgaagagttg ctgaagcttg
    17941 acgcgctggc gaatcaacac ggcattccgc tggtgattga taacgcttat ggcgtcccgt
    18001 tcccgggtat catcttcagt gaagggcgcc cgctatggaa tccgaatatc gtactgtgca
    18061 tgagtctttc caagctgggg ctgcctggct cccgctgcgg cattatcatc gccaatgaaa
    18121 aaatcatcac tgccatcacc aatatgaacg gcattatcag cctggcacct ggcggtattg
    18181 gtccggcgat gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg tctgaaacag
    18241 tcatcaaacc gttttactac cagcgtgttc aggaaactat cgccatcatt cgccgctatt
    18301 taccggaaaa tcgctgcctg attcataaac cggaaggagc cattttcctc tggctatggt
    18361 ttaaggattt gcccattacg accgagcagc tctatcagcg cctgaaagca cgcggcgtgc
    18421 tgatggtgcc ggggcacaac ttcttcccgg gactggataa accgtggccg catacgcatc
    18481 aatgtatgcg catgaactac gtaccagagc cggagaaaat tgaggcgggg gtgaagattc
    18541 tggcggaaga gatagaaaga gcctgggctg aaagtcacta aaaattgcct gatgcgctac
    18601 gcttatcagg cctacatgat ttctgcaata tattgaattt ggaagaattt gtaggccgga
    18661 taaggcgttt acgccgcatc tggcataaac aaagcgcact attccggcgt tgagaaacgc
    18721 cggaaaacgt ttcaaacagg ctgctgttgt agccgatgcc gctgcacttt ctcgacatcc
    18781 atgcactgca acgcctttgt cgggcaggct tcaacacagg ccggccccgt ctcccgatgc
    18841 cagcacagat cgcacttaat cgccctcgcc tttttgcgcg aagaaacgac ctccatcgca
    18901 ccaaacgggc aagccagcat acagctttta cagccaatgc aacgtgtttg ttcaacgaaa
    18961 atatgcccat gttcgcggct tatcgcgtca acagggcaga cattcgcgca cggtgcatct
    19021 tcacactgat gacaggctac tggcgtggtc cagctgtggt ctttaatgac acgaatacgg
    19081 gaaataaact cgtctggtga caacgcagcg caatcctgat tctcctgatg cgacactgcg
    19141 caagccactt cacaggtacg gcaaccgata catttcgtcg catccgcaat aataaaccgg
    19201 ttcatgatca ttcctttgca ctaaaacagc agagtgccag agagcgcgtc cctactgctt
    19261 tgatccaacg aaggaaatgt taaagttttg gcacttgtcc aaagcgttat gtgatcgcgc
    19321 tcaggtcatc cctgacagta aatcccagtt cattagaaat agcctgcgcg gtttcacgca
    19381 gtggtttcag gagatttttc tctcccacct gtttcagacg tgatgtcgaa agcgaaatcg
    19441 acacggcgta cggcacccgc ccatgaatat caaacaccgg aacagcaata caggagacgc
    19501 cgagttcgtt ttcttctctg tccatcgccg ctccgctttc acgaatgtgc gccagttcgt
    19561 cgaacatcgc gggcagctcg gtaatggtat tgcgggttaa cggctggatc tcatgctgat
    19621 ggttttccca gtatgacttc acgtagtccg ggtgaccaaa cgccatgtag atcttgccca
    19681 ttgcggaaca gtagagcggc atatgctggc caatataggc acgggttcgc agcatcccgg
    19741 ttgtgggttc cagcttataa atcaaaatag cgtgatcgtc ttcgcggctg gagaagttaa
    19801 tggtttcacc agtggcgatg ttcagtgcct caagatgcgg agcggcgata tgaatgatat
    19861 tcagcgaaga cagcgccttc tgcccgacgg caataaattt ggtagtcagg cggtaactcc
    19921 ctgcggcggg cgcggtggtc acatagccgc aggactgtaa tccctgcaat aagcgatgga
    19981 cggtactctt atttaaacca gccagctccg aaagatgcgc caacggacaa ccgtttggat
    20041 agttgctcaa aatctcaatc agcatcaacc cgcgaaacag actctggctt ccggctggac
    20101 gctctttttc ctgcgccatc tcgttctctt tttttcccat cacttctttc cccattttgt
    20161 cgcgtcctga tggtagcgca aagtgtgccg tagttcacga tctcgacaga taatttataa
    20221 ccacttgatt tttatgtctt ttgaaattca tcaatcagat tgctttgtta aaaagtgatc
    20281 gatatatttg aaatctagtt ccgcatattg aaattttaag ccaaaaaagc gatcaaaaaa
    20341 acaaggaagc ctaggatgaa agtgacattt gagcagttaa aagcagcctt taatcgggtc
    20401 ttaatttcac gcggcgttga cagcgaaacg gctgacgcct gtgcagagat gttcgcccgc
    20461 accaccgaat ccggcgttta ttctcacggc gttaatcgtt tccctcgttt cattcaacaa
    20521 ctggaaaacg gcgatatcat tcctgatgcc caacccaaac gtataaccag cctcggcgca
    20581 attgaacagt gggatgccca gcgttcgatc ggcaacctga cggcgaaaaa gatgatggat
    20641 cgcgccattg aactggctgc cgatcacggt attggtctgg tggcacttcg taatgccaac
    20701 cactggatgc gcggcggcag ctacggctgg caggcggcgg aaaaaggcta tattggcatt
    20761 tgctggacca actccatcgc cgtaatgccg ccgtggggcg caaaagagtg tcgcatcggc
    20821 actaacccgc tgatcgtcgc cattccttcc acgccgatca ccatggtcga tatgtcgatg
    20881 tcgatgttct cttacggcat gttagaagtt aaccgcctgg caggccgtca gctcccggtc
    20941 gatggtggct ttgatgatga gggcaatttg accaaagaac ctggcgttat cgagaagaat
    21001 cgccgcattt tgccgatggg ctactggaaa ggttctggca tgtcgattgt gctggatatg
    21061 atcgctactc tcctttccga cggcgcatcg gttgccgaag tcacccagga caacagcgac
    21121 gaatacggca tttcacaaat ttttattgcc attgaagtgg acaagcttat cgacggtccc
    21181 acccgcgatg ccaagctgca acgtatcatg gattacgtta ctaccgctga acgcgctgac
    21241 gaaaaccagg ccatccgctt acccggccac gaatttacta ccctgctggc cgaaaaccgc
    21301 cgtaacggca tcactgttga tgacagcgtg tgggccaaaa tccaggcgtt atgaggagat
    21361 aagtcatgat ttttggacat atcgcgcagc ccaatccgtg ccgtttgccc gccgccattg
    21421 aaaaggcgct cgattttctg cgcgctacca acttcaacgt cctggagccg ggcgttgtcg
    21481 aaatcgacgg caagaatatt tatgcgca
//
LOCUS       DAJTUN010000065        21445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTUN010000065 DAJTUN010000000
VERSION     DAJTUN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.065"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(119..1072)
                     /gene="tal"
                     /locus_tag="OO470_002279"
     CDS             complement(119..1072)
                     /gene="tal"
                     /locus_tag="OO470_002279"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6251507.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1351..2774
                     /locus_tag="OO470_002280"
                     /pseudo
     CDS             1351..2774
                     /locus_tag="OO470_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            2844..3620
                     /gene="yaaA"
                     /locus_tag="OO470_002281"
     CDS             2844..3620
                     /gene="yaaA"
                     /locus_tag="OO470_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6251508.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(3773..4069)
                     /locus_tag="OO470_002282"
     CDS             complement(3773..4069)
                     /locus_tag="OO470_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6251509.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4283..5569)
                     /gene="thrC"
                     /locus_tag="OO470_002283"
     CDS             complement(4283..5569)
                     /gene="thrC"
                     /locus_tag="OO470_002283"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6251510.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(5570..6502)
                     /gene="thrB"
                     /locus_tag="OO470_002284"
     CDS             complement(5570..6502)
                     /gene="thrB"
                     /locus_tag="OO470_002284"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6251511.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(6504..8966)
                     /gene="thrA"
                     /locus_tag="OO470_002285"
     CDS             complement(6504..8966)
                     /gene="thrA"
                     /locus_tag="OO470_002285"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6251512.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(8992..9111)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9047..9112)
                     /gene="thrL"
                     /locus_tag="OO470_002286"
     CDS             complement(9047..9112)
                     /gene="thrL"
                     /locus_tag="OO470_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6251513.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9326..10012)
                     /locus_tag="OO470_002287"
     CDS             complement(9326..10012)
                     /locus_tag="OO470_002287"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6251514.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(10412..10552)
                     /locus_tag="OO470_002288"
     CDS             complement(10412..10552)
                     /locus_tag="OO470_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251515.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            10648..11364
                     /gene="arcA"
                     /locus_tag="OO470_002289"
     CDS             10648..11364
                     /gene="arcA"
                     /locus_tag="OO470_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6251516.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(11423..12775)
                     /gene="creD"
                     /locus_tag="OO470_002290"
     CDS             complement(11423..12775)
                     /gene="creD"
                     /locus_tag="OO470_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6251517.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(12833..14257)
                     /gene="creC"
                     /locus_tag="OO470_002291"
     CDS             complement(12833..14257)
                     /gene="creC"
                     /locus_tag="OO470_002291"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6251518.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14257..14946)
                     /gene="creB"
                     /locus_tag="OO470_002292"
     CDS             complement(14257..14946)
                     /gene="creB"
                     /locus_tag="OO470_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6251519.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(14959..15432)
                     /gene="creA"
                     /locus_tag="OO470_002293"
     CDS             complement(14959..15432)
                     /gene="creA"
                     /locus_tag="OO470_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6251520.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            15643..16512
                     /gene="robA"
                     /locus_tag="OO470_002294"
     CDS             15643..16512
                     /gene="robA"
                     /locus_tag="OO470_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6251521.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(16509..17156)
                     /gene="gpmB"
                     /locus_tag="OO470_002295"
     CDS             complement(16509..17156)
                     /gene="gpmB"
                     /locus_tag="OO470_002295"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6251522.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17208..17728
                     /gene="yjjX"
                     /locus_tag="OO470_002296"
                     /pseudo
     CDS             17208..17728
                     /gene="yjjX"
                     /locus_tag="OO470_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(17813..18139)
                     /gene="trpR"
                     /locus_tag="OO470_002297"
     CDS             complement(17813..18139)
                     /gene="trpR"
                     /locus_tag="OO470_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6251523.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18229..20166)
                     /gene="sltY"
                     /locus_tag="OO470_002298"
     CDS             complement(18229..20166)
                     /gene="sltY"
                     /locus_tag="OO470_002298"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6251524.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20377..>21445
                     /gene="ettA"
                     /locus_tag="OO470_002299"
     CDS             20377..>21445
                     /gene="ettA"
                     /locus_tag="OO470_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6251525.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIG"
BASE COUNT         5115 a         5806 c         5299 g         5225 t
ORIGIN      
        1 tcatcaagat ttttccgttt aaacaatctc gttaaacaga atgatacact gcgaagggag
       61 tgacagacag gcttcagaag aggtagcgtg accgacttcc cggtcacgct aagaatgatt
      121 acagcagatc gccgatcatt ttttccagtt tttcctggtc aatagcaaac ttacggatac
      181 cttccgccag tttatctact gccattggat cctggttgtg ctgccacagg aactcggact
      241 cagtgatacg cgccggacgc gctttcactt cgccggtgta agacagttta cgttcgatag
      301 ccccttcgct ctccgccagc tctttcagca gtgccggtgc gatggtcaga cggtcacagc
      361 ctgccagttc cagaatttcg ccgatgttac ggaagcttgc gcccataacc acggtttcat
      421 aaccgtgctc tttgtagtac tggtagattt cagatacaga aaccacgccc ggatcttctg
      481 ccggagcgta ctctttctta tcggtattcg ctttgtacca gtcaagaata cggccaacaa
      541 acggcgagat caggaacacg cccgcctccg cacaagcacg agcctgagcg aaggagaaca
      601 gcagggtcag gttacagttg ataccttctt tttccagctg ttctgcagca cggataccct
      661 gccaggtaga agccagtttg atcagaatac gatcgttgct aatacctgca tcgttgtaga
      721 gtttgatcag gcgttttgct ttcgcaattg acgcttcggt gtcataggaa agacgcgcat
      781 caacttcagt tgagatacgg cccggaacca gtttcaggat ttccagacca atatttactg
      841 ccagtttgtc ggtcgcgtcc acgatctgct gcgcgcgatc gttgctctgc tgtttcgccc
      901 aggcgacagc atcatcaatc aacttacggt attccggaat ctgcgctgcg ttaagaatga
      961 gagaagggtt ggttgtggca tcctgcggtt gatacagctt cattgccgcg atgtccccag
     1021 tgtcggccac tacggtggtg tactgacgaa gggaggtcaa tttgtccgtc atgatagtat
     1081 ttctctttaa acagcttgtt agggggatgt aaccggtctg ccctgatgat atcacgacgc
     1141 aacgtcagcg caaccgccga caagacggtt atcgccaggc gagactgttt cggatttctg
     1201 acactgcgtt gatatcgccc accattttta tacaaaacct catgtatgct acgcagaagt
     1261 tatcaagtac ctcgtagcgt atatacttct taaacaattg gtaacgttta cacaggaaag
     1321 tcatcgcgac cggcaataag agggatatgc atgccagatt ttttctcctt tattaacagc
     1381 gtcctttggg gatcggtaat gatttacctg ctcttcggcg caggttgttg gttcactttt
     1441 cgcaccggat ttgtgcagtt tcgctacatc cgccagtttg gcaaaagtct taaaaatagc
     1501 attcatccac agccaggcgg tttaacctca tttcagtcat tgtgtaccag tcttgcggcg
     1561 cgcgtgggta gcggcaacct ggccggcgtt gcgctggcaa ttaccgccgg tggaccaggt
     1621 gccgtctttt ggatgtgggt tgctgcgttt atcggcatgg cgacctcgtt tgccgaatgt
     1681 tcccttgcac aactttataa agaacgtgac gtcaatgggc agtttcgtgg cggaccggca
     1741 tggtatatgg cgcgcgggct ggggatgcgc tggatgggcg ttctgttcgc cgtctttttg
     1801 ctcatcgcct acggcataat tttcagcgga gttcaggcga acgccgttgc ccgagccctg
     1861 agtttttctt ttgattttcc tccgctggtg acgggcatta ttctcgctgt ctttgctctg
     1921 ctggcaatca ctcgcggtct tcatggcgtc gcccggctca tgcaggggtt tgtcccgttg
     1981 atggcgataa tctgggtact gaccagcctg gtgatttgcg taataaatat cgggcaactt
     2041 ccccacgtca tttggtctat ttttgagagt gcttttggct ggcaggaagc ggcaggcggc
     2101 gcggcgggat ataccttaag ccaggcgatt actaacggtt ttcagcgcag tatgttttcc
     2161 aatgaggtgg gaatgggatc gacgccaaac gcggcagctg catcctggcc tccgcatccg
     2221 gcagcacaag ggattgtcca gatgattggc atttttatcg acaccctggt catctgtacg
     2281 gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc gctggaaggt
     2341 attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctagggtgc tgaatttgtt
     2401 accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa
     2461 aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc
     2521 accttcgcaa cgatcatcgg cggcaccttg ctaagccttc cgctgatgtg gcaactggca
     2581 gatatataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
     2641 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
     2701 ttgatccgtt gcgttatccg gatatcggtc gccaactttc tccggacgcg tgggatgatg
     2761 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
     2821 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
     2881 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
     2941 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
     3001 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
     3061 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
     3121 ccgagacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
     3181 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
     3241 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
     3301 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
     3361 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
     3421 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
     3481 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
     3541 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
     3601 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
     3661 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
     3721 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
     3781 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
     3841 gcggtgacgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
     3901 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
     3961 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
     4021 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
     4081 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
     4141 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
     4201 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
     4261 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
     4321 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
     4381 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
     4441 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
     4501 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
     4561 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
     4621 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
     4681 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
     4741 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
     4801 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
     4861 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
     4921 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
     4981 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
     5041 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
     5101 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
     5161 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
     5221 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
     5281 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
     5341 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
     5401 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
     5461 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
     5521 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
     5581 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
     5641 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
     5701 cgaacaaggt cgggccggag ccggagatac cgctcactac cgcgccgatt tccgcgaccg
     5761 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
     5821 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
     5881 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
     5941 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
     6001 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
     6061 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
     6121 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
     6181 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
     6241 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
     6301 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt
     6361 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
     6421 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
     6481 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
     6541 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
     6601 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
     6661 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
     6721 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
     6781 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
     6841 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
     6901 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
     6961 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
     7021 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
     7081 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
     7141 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
     7201 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg taacaacgtg gaaaccttcg
     7261 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
     7321 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
     7381 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
     7441 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
     7501 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
     7561 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
     7621 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
     7681 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
     7741 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
     7801 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
     7861 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
     7921 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
     7981 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
     8041 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
     8101 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
     8161 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
     8221 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
     8281 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
     8341 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
     8401 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
     8461 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
     8521 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
     8581 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
     8641 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
     8701 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
     8761 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
     8821 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
     8881 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
     8941 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
     9001 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
     9061 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
     9121 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
     9181 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
     9241 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
     9301 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
     9361 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
     9421 cgaccagttt tatgtcatct gccactgcca gagccgtcag caatgccatg actcgttcgc
     9481 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
     9541 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
     9601 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
     9661 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
     9721 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
     9781 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
     9841 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
     9901 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
     9961 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    10021 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    10081 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    10141 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    10201 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    10261 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    10321 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    10381 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    10441 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    10501 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    10561 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    10621 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    10681 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    10741 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    10801 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    10861 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    10921 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    10981 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    11041 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    11101 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    11161 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    11221 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    11281 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    11341 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    11401 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    11461 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    11521 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    11581 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg ataaacaaca
    11641 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    11701 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    11761 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    11821 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    11881 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    11941 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    12001 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    12061 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    12121 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    12181 tattcagctg caggttctgc ttacgccagt ccccttcggg taaagggata tgcacgccct
    12241 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    12301 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    12361 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    12421 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    12481 tctgattgcc atcaaccatc aatgactcag gtaacaaaaa atggataaaa cttcgtttcc
    12541 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    12601 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    12661 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    12721 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    12781 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    12841 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    12901 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    12961 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    13021 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    13081 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    13141 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    13201 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    13261 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    13321 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    13381 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    13441 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    13501 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    13561 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    13621 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    13681 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    13741 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    13801 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    13861 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    13921 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    13981 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    14041 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    14101 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    14161 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    14221 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    14281 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    14341 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    14401 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    14461 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    14521 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    14581 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    14641 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    14701 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    14761 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    14821 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    14881 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    14941 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    15001 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    15061 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    15121 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    15181 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    15241 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    15301 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    15361 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    15421 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    15481 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    15541 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    15601 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    15661 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    15721 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    15781 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    15841 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    15901 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    15961 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    16021 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    16081 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    16141 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    16201 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    16261 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    16321 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    16381 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    16441 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    16501 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    16561 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    16621 atagaacagt tacgcagacg taagcggcgc tctacccatg ctggtaatcc gagaatcgta
    16681 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    16741 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    16801 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    16861 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    16921 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    16981 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    17041 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    17101 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    17161 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    17221 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    17281 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    17341 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    17401 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    17461 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    17521 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    17581 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    17641 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    17701 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    17761 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    17821 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    17881 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    17941 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    18001 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    18061 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    18121 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    18181 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    18241 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    18301 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    18361 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    18421 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    18481 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    18541 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    18601 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    18661 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    18721 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    18781 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    18841 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    18901 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    18961 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    19021 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    19081 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    19141 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    19201 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    19261 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    19321 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    19381 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    19441 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    19501 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    19561 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    19621 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    19681 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    19741 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    19801 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    19861 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    19921 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    19981 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    20041 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    20101 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    20161 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    20221 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    20281 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    20341 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    20401 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    20461 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    20521 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    20581 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    20641 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    20701 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    20761 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    20821 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    20881 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    20941 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    21001 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    21061 atcctcgaac tggaccgcgg tgaaggtatt ccgtgggaag gtaactactc ctcctggctg
    21121 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    21181 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    21241 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    21301 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    21361 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    21421 ggggcgatcg tcgggatcat cggtc
//
LOCUS       DAJTUN010000066        21340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTUN010000066 DAJTUN010000000
VERSION     DAJTUN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.066"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..87)
                     /locus_tag="OO470_002300"
     CDS             complement(<1..87)
                     /locus_tag="OO470_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251526.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDA"
     gene            complement(189..1169)
                     /locus_tag="OO470_002301"
     CDS             complement(189..1169)
                     /locus_tag="OO470_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6251527.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1177..1887)
                     /locus_tag="OO470_002302"
     CDS             complement(1177..1887)
                     /locus_tag="OO470_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6251528.1"
                     /translation="MVENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2104..3927)
                     /gene="typA"
                     /locus_tag="OO470_002303"
     CDS             complement(2104..3927)
                     /gene="typA"
                     /locus_tag="OO470_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6251529.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4300..5709
                     /gene="glnA"
                     /locus_tag="OO470_002304"
     CDS             4300..5709
                     /gene="glnA"
                     /locus_tag="OO470_002304"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6251530.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5882..6931
                     /gene="glnL"
                     /locus_tag="OO470_002305"
     CDS             5882..6931
                     /gene="glnL"
                     /locus_tag="OO470_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6251531.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6943..8352
                     /gene="glnG"
                     /locus_tag="OO470_002306"
     CDS             6943..8352
                     /gene="glnG"
                     /locus_tag="OO470_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6251532.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8464..8574
                     /locus_tag="OO470_002307"
     CDS             8464..8574
                     /locus_tag="OO470_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6251533.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8803..10176)
                     /gene="hemN"
                     /locus_tag="OO470_002308"
     CDS             complement(8803..10176)
                     /gene="hemN"
                     /locus_tag="OO470_002308"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6251534.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10365..10874)
                     /gene="yihI"
                     /locus_tag="OO470_002309"
     CDS             complement(10365..10874)
                     /gene="yihI"
                     /locus_tag="OO470_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6251535.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11456..12088
                     /locus_tag="OO470_002310"
     CDS             11456..12088
                     /locus_tag="OO470_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6251536.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12470..15256)
                     /gene="polA"
                     /locus_tag="OO470_002311"
     CDS             complement(12470..15256)
                     /gene="polA"
                     /locus_tag="OO470_002311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6251537.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15620..16552
                     /locus_tag="OO470_002312"
     CDS             15620..16552
                     /locus_tag="OO470_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6251538.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16593..18023)
                     /locus_tag="OO470_002313"
     CDS             complement(16593..18023)
                     /locus_tag="OO470_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6251539.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFFFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18178..18804)
                     /gene="dsbA"
                     /locus_tag="OO470_002314"
     CDS             complement(18178..18804)
                     /gene="dsbA"
                     /locus_tag="OO470_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6251540.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18821..19807)
                     /gene="srkA"
                     /locus_tag="OO470_002315"
     CDS             complement(18821..19807)
                     /gene="srkA"
                     /locus_tag="OO470_002315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6251541.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCYAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19884..20153)
                     /locus_tag="OO470_002316"
     CDS             complement(19884..20153)
                     /locus_tag="OO470_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6251542.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20223..20807
                     /gene="mobA"
                     /locus_tag="OO470_002317"
     CDS             20223..20807
                     /gene="mobA"
                     /locus_tag="OO470_002317"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6251543.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20789..21316
                     /gene="mobB"
                     /locus_tag="OO470_002318"
     CDS             20789..21316
                     /gene="mobB"
                     /locus_tag="OO470_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6251544.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5219 a         5358 c         5131 g         5632 t
ORIGIN      
        1 cgcatctttc agcgacggta atttataaat tgtaccgcca cacagtgtta atagactaaa
       61 taacgcccat ttctttttcg tgagcataac atcccctaag cgatttttaa aatcactgtt
      121 ttaaaggcaa taagataccg accattcata tggaatggtt ttattaactg cgtatgagtt
      181 aaaacttatt agtggttcag aagatggttt gcctcttgtt tgatttgttg tagtacgtta
      241 cgcacatgat ttatctgatt aattgcatct tctttttctt tacgtaaccg cgcatccagt
      301 aaatgatttt ctggtagggg tgtttcactc atctggcgat aagggatcca cggattatca
      361 tcttcgtctt caaagcggaa cgccgacgca tagtaatcaa cctcttcttc caggccatat
      421 gccgtcacct gcggctcatc atcgccaagg cagataaggt gcgttaataa cgctttgaat
      481 ggcatatcac cctgacctga aatacaggct ttatgaccca ggccaccctg ttctttaacg
      541 atcaaggcat ctttaatatg gacctgggta atatgcggtg ccatcgtttt taaggcgtcg
      601 atgggatgct catttgcatt gatcatgtta gcaaaatcaa acagtaagga aagagactcc
      661 atctcacttt ctttgaccag cgacaccagc tcatgacttt ttaaatcttc atgctgctca
      721 atcgtaaaag tcaggccgct gtcctgatac gtttcccgta cataggcaat gtcgttagcg
      781 ataatcgata atacgtcgcg caaattacct tcataacgtg gataaaaacg tacggacgtt
      841 gccccagttt tcaacgcgat ggcgacggct tcgtcaatag atgccttatc tgaagcgctg
      901 gtttcaatat gaatatcaag gttcagacgg cgggctttat ccccaaaggc agagagttct
      961 ttatcgtcca tattaccaag agaaaaacgc tcgccatcca gaacatggat tttgacgcca
     1021 cgtagattat tttcacttgc gatatcaagt aaatcagcag gcaacactct ttccagccgc
     1081 atatttaaat gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga
     1141 atctttcttg cgttatttat tgtcaccata aaacacctac agattaatga tgaggtctac
     1201 ggcctcaaat cgccaatatt caatatctat ctggacaata ttgccttgct ggtcggcgcg
     1261 atgcttttca attaagatcg ctggcatacc cgcagaaccg ccaagatact tgctgatatc
     1321 gcccggcatt ctgacgggtt tgaaaatcaa tttttttact accgtctctt tttggtagca
     1381 cttttcccag actgcagaaa atgagtggtt ttcaagttgt tcaataaaac ccggagcaac
     1441 ttcaggatta atatatgttt cgtgatagaa aactttatgt ccttccagcg ccccccagcc
     1501 ggtgatgcga tagagttcag ttgatggcgt cactgcaatc agtggcgcga gcgtctcggg
     1561 aatatcgctg gtacggtttt tctcggtaaa cccccaagaa ggctctcgcc cttgctcaat
     1621 tgcggcccgc tgaaagctcg ccgacagctc cggactgtaa ttaaatcgcg gctgggtcac
     1681 gaaccacccc ttacgatcct tacggaagat tttggattct gcctcgaggt tcaacaacgc
     1741 ctggcgcagc gtcatacgtt taatgcccag cagttctccc agctctcttt ctgaagggag
     1801 ttttccaccc ggcgtggtga tgccatcttt caaccaccga tccagtttct cttttgcatt
     1861 ttctacggtg gattgattct ccaccattgt ttaatccttt tggtttaaac cagatgaagc
     1921 atcttacgct tacgccaaaa aagaaagcaa ttaacggtgt atgactttgc agataattgt
     1981 gacaaagctc tctattgacg taatcacaga cttataaggg agatactcgc attggtgtgc
     2041 gctcacgaca ggtaaaaaaa aacctgccat cgctggcagg ttttttatga ctaaaaaacg
     2101 aaattaatcg tctttcggtg cgcggttggc gcggcgacga tcgttttccg tcaggtgacg
     2161 tttacgaata cggatagagg tcggagtcac ttctaccagt tcgtcatcat cgatgaactc
     2221 cagagcttgt tccagagtca tgcggatagg cggaaccaga acaacagctt cgtcagtacc
     2281 ggaagcacgc atgttggtca gtttcttacc ggtcaggcag tttacagtca ggtcgttaga
     2341 gcggctatga ataccgataa tctgaccttc gtaaacttct gcaccgtgac cgaggaacag
     2401 cttaccgcga tcctgcagac cgaacagcgc gaacgcgaca gctttaccct gaccgttaga
     2461 gatcagtaca ccgttctgac gctgacccac ttcgcccgga cgtacgtcgt cgtagtggct
     2521 gaaggtggag tacagcagac cagtaccgga agtcatggtc atgaactcag aacggaagcc
     2581 aatcagacca cggcttggga tcacgtagtc gagacgtacg cggcctttac cgtctggatt
     2641 catgtttttc aggtcgcctt tacgttcgcc cagcgcctgc atcacagaac cctgatgctg
     2701 ttcttcaacg tccagcgtca cgttttcata cggctcttgt ttacgaccgt ctatttcacg
     2761 gaagattact ttcggacggg ataccgccag ttcgaaacct tcacgacgca tgttttcgat
     2821 aagaacagag aggtgcagtt caccacgacc agaaacgcgg aacgcatcgg cgtcttcggt
     2881 ttcttctacg cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat cgaggatctg
     2941 acgagacgtt acgaacttac cttctttacc gcagaacggc gaggtgttaa cgcagaagaa
     3001 catagaaacg gtcggctcat caacggagag tgccggcagc gcttcaacgt tttgcgtgtc
     3061 gcaaacggtg tcagaaatgt tcagttcgcc caggcccgtg atcgccacga tatcgccagc
     3121 ttccgccaga tcggtttcga tacgttccag accgaggtgg cccagcactt taccgacttt
     3181 cgcgttacgg gttttgcctt cgctatcgat gatagtaacc tgctggttcg gcttcacttt
     3241 accacgcttg atgcggccaa tgccgataac gccaacatag ctgttgtaat cgagctgaga
     3301 aatctgcatc tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt ggtcaacaat
     3361 cgcctgatac agcggggtca tgtcttccgc catatcttcg tggtccagac ccgcgatacc
     3421 gttcagcgca gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt
     3481 aacgaacaga tcgaatacct gatccacaac ccaatcagga cgcgcgccag ggcggtcaac
     3541 tttgttgata acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg
     3601 cgtttgcggc atcgggccgt caaatgcgtc aaccaccagc agcactgagt ctaccatgga
     3661 cattacacgt tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat
     3721 acggtaatca ttccatttga tagcggtgtt tttcgcgagg atggtaatcc cacgctcttt
     3781 ctccaaatcg ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc
     3841 ggattgttgg agcagcttgt ctaccagggt ggttttacca tggtctacgt gcgcgatgat
     3901 ggcgatatta cgcaattttt cgatcacaac tttgcctcag gcattagaaa tagcgcgtta
     3961 ttgtacacgg attaatcgca ctacaaaaca ggatcacaaa catcctccgc aaacaagtat
     4021 tgcagagtcc ctttgtgatc gctttcacgg agcataaaaa gggttatcca aaggtcattg
     4081 caccaatatg gtgcttaatg tttccattga agcactatat tggtgcaaca ttcacatcat
     4141 ggtgcagccc ttttgcacga tgttaagcat gataacgcct tttaggggca atttaaaagt
     4201 tggcacagat ttcgctttat cttttttacg gcgacacggc caaaataatt gcagatttcg
     4261 ttaccacgac gacaatgacc aatccaggag agttaaagta tgtccgctga acacgtactg
     4321 acgatgctga acgagcacga agtgaagttt gttgatttgc gcttcaccga taccaaaggt
     4381 aaagaacaac acgtcactat ccctgctcat caggtgaatg ctgaattctt cgaagaaggc
     4441 aaaatgtttg acggctcctc gattggcggc tggaaaggca ttaacgaatc cgacatggtg
     4501 ctgatgccag acgcatccac tgcagtgatt gacccgttct tcgccgactc caccctgatt
     4561 atccgttgcg acatccttga acctggcacc ctgcaaggct atgaccgtga cccgcgctct
     4621 attgcgaagc gcgccgaaga ttacctgcgt tccactggca ttgccgacac cgtactgttc
     4681 gggccagaac ctgaattctt cctgttcgat gacatccgtt tcggatcatc tatctccggt
     4741 tcccacgttg ctatcgacga tatcgaaggc gcatggaact cctccaccca atacgaaggt
     4801 ggtaacaaag gtcaccgtcc ggcagtgaaa ggcggttact tcccggttcc gccggtagac
     4861 tctgctcagg atattcgttc tgaaatgtgt ctggtgatgg aacagatggg tctggtggtt
     4921 gaagcccatc accacgaagt agcgactgct ggtcagaacg aagtggctac ccgcttcaat
     4981 accatgacca aaaaagctga cgaaattcag atctacaaat atgtcgtgca taacgtagcg
     5041 caccgcttcg gtaaaaccgc gacctttatg ccaaaaccga tgttcggtga taacggctcc
     5101 ggtatgcact gccacatgtc tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa
     5161 tacgcaggtc tgtctgagca ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa
     5221 gcgattaacg ccctggcaaa cccgaccacc aactcttata agcgtctggt cccgggctac
     5281 gaagcaccgg taatgctggc ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg
     5341 gtggtttctt ctccgaaagc acgtcgtatc gaagtacgtt tcccagatcc ggcagctaac
     5401 ccgtacctgt gctttgctgc cctgctgatg gccggtcttg atggtatcaa gaacaagatc
     5461 catccgggcg aagccatgga caaaaacctg tatgacctgc cgccagaaga agcgaaagag
     5521 atcccacagg ttgcaggctc tctggaagaa gcactgaacg aactggatct ggaccgcgag
     5581 ttcctgaaag ccggtggcgt gttcacggat gaagcgatcg atgcgtacat cgctctgcgt
     5641 cgcgaagaag atgaccgcgt gcgtatgact ccgcatccgg tagagtttga gctgtactac
     5701 agcgtctaat agttgaagtt gtactacccg gcgcacaacg ccgggattta gttgccgtgg
     5761 aaactttcag cccatctctg catgggcttt tttctccgtc aattctctga tgcttcgcgc
     5821 tttttatccg taaaaagcta taatgcacta aaatggtgca acctgttcag gagactgctt
     5881 tatggcaaca ggcacgcagc ccgatgctgg gcagatcctc aactcgctga ttaacagtat
     5941 tttgttaatt gatgacaatc tggcgatcca ttacgccaac cccgccgcgc aacaactgct
     6001 cgcccaaagc tcccgcaaat tgtttggtac gccgttaccg gaactgttga gctacttctc
     6061 attaaatatc gagctgatgc aagaaagtct ggaggcgggg caaggcttta ccgataacga
     6121 agtgacgctg gtcatcgatg gtcgctcgca tatcctttct gtgacggccc agcgtatgcc
     6181 ggacggcatg atcctgctgg agatggcacc gatggataac cagcgccgct taagccagga
     6241 acaactacaa catgctcaac aagtagctgc ccgtgattta gtgcgcggcc tggcacatga
     6301 gattaaaaat ccgcttggcg gtttacgtgg cgcggcgcag ttactcagca aagcgttacc
     6361 tgacccatca ctactcgaat ataccaaagt gattatcgaa caggcggacc ggctgcgaaa
     6421 tttggtcgac cgtctgttgg ggccgcagct gcccggtacg cgcgttaccg aaagtattca
     6481 caaagtggct gaacgcgtgg taacgctggt gtcgatggaa ctgccggaca acgtgcggtt
     6541 gattcgtgat tacgatccca gcctgccgga actggcgcac gacccggatc aaattgaaca
     6601 ggtcttgctg aatattgtgc gcaatgcgct acaggcgctg gggccggaag gcggtgaaat
     6661 cattctgcgt acccgcaccg cgtttcaact gaccttacac ggcgagcgct accggctggc
     6721 ggcgcggatt gatgtggaag ataacgggcc gggcattccg cctcatttgc aggatacgct
     6781 gttttacccg atggtcagcg gccgcgaagg tggcaccggg cttggcttat ccatcgcccg
     6841 taatttgatt gatcagcatt caggcaaaat tgaatttacc agttggccag gtcataccga
     6901 gttctcggtt tacctgccta tcaggaaata aaggtgacgt ttatgcaacg agggatagtc
     6961 tgggtagtcg atgacgatag ttccatccgt tgggtgcttg aacgtgcgct cgctggagcg
     7021 ggtttaacct gtacgacatt tgagaacggc gcggaagtac tggaggcgct ggcgagcaaa
     7081 acgccggatg tgctgctttc agatatccgt atgccgggaa tggacgggct ggcgctgctc
     7141 aagcagatta aacagcgcca tccaatgctt ccggtcatca ttatgaccgc acattccgat
     7201 ctggatgctg ccgtcagcgc ctatcaacaa ggggcgtttg attatctgcc caaaccgttt
     7261 gatatcgacg aagcagtggc gctggttgag cgcgctatca gtcattacca ggaacagcag
     7321 cagccgcgta atattcagct taacggccca acgaccgata tcatcggcga agcgcaagcc
     7381 atgcaggacg tgttccgtat tatcggtcgg ctttcgcgtt cttctattag cgtgctgatt
     7441 aacggcgaat ccggcaccgg taaagaactg gtcgctcatg ccctgcatcg ccacagtccg
     7501 cgcaccaaag cgccgtttat cgcgctgaat atggcagcta tcccaaaaga tttgatcgaa
     7561 tcagaactgt ttggccacga gaaaggcgcg tttactggcg cgaataccat tcgtcagggg
     7621 cgttttgaac aggccgatgg cggcacgtta tttctcgacg aaattggtga tatgccgctg
     7681 gatgtgcaga cgcgtttgct gcgcgtgctg gcagacggtc agttttatcg cgttggcggc
     7741 tatgcgccgg tgaaagtgga tgtgcggatt atcgctgcca ctcaccagaa tcttgaacag
     7801 cgggtgcagg aaggtaagtt ccgtgaggat ctgttccacc gcctgaacgt tatccgcgtt
     7861 catctgccgc cgctgcgcga acgtcgggaa gatattcccc gtctggcgcg ccatttttta
     7921 caggttgccg cgcgcgaact gggcgtagaa gcgaagttgc tgcatccgga aaccgaagct
     7981 gctctgacgc gtctggcgtg gccaggcaac gtgcgccagc tggaaaacac ctgccgctgg
     8041 ctaacggtga tggccgccgg gcaggaagtg ttgattcagg atttgcccgg cgaactgttt
     8101 gaatcaacgg ttgcggagag tacttcgcaa atgcaaccgg acagttgggc gacactttta
     8161 gcgcagtggg cagacagagc gctgcgttcc ggtcatcaaa atctgctttc cgaagcgcag
     8221 ccagagctgg agcggacgtt actgacgacc gcgttgcgac atacgcaggg gcataaacag
     8281 gaagcggcgc ggctactcgg ctggggccgc aacactctga cgcgtaagtt aaaagagctg
     8341 gggatggagt gattcacggc ttgtgtgtaa agattgatta ttgagcgcaa attgctggta
     8401 ttttgcgctt tactgttccg ataagttcag tatgatcttg cccggaaaac ggggagagtc
     8461 attatgctgg aatcaataat taatctggta tcgagtggcg cagttgacag ccacacaccg
     8521 caaactgccg ttgctgccgt gctgtgtgcc gcgatgattg ggctgtttag ctgaagctcg
     8581 ttgtttatgc cggatgcgga gaaagtcgta ggcctgataa gacgcgacca gcgtcgcatc
     8641 aggcatcatg ctcagatgcc agatgcggcg taaacgcctt atccggccta caaaagcaat
     8701 aatttcaaca ggttttgttg cccagcgcat caggcaatct tgttctcagc acattgatgg
     8761 cgctttcgtt ttctactttg taaacgaagc gccattcact ctttaaatca cccgtgagaa
     8821 ctgctgcatc cgcgctttct ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat
     8881 cagcaagcga cctttcgccg tcacctgtat tcccttctca tccacatcca ccagcccatc
     8941 ttttgctaac ggggcgagca gcttgagatc ttcctcaaag taatcagcga agtgcaaatc
     9001 ccactgtttc tcaagagggg cgtaatccag acggaagttg cagatgagcg acttaatcac
     9061 atcgcggcga atacagtcat cacgcgttag cgcaatacca cgccacagcg cattgccttg
     9121 ttcatacact tgctgatagt actgcttcaa ctctttctgg ttctgcgcgt agcagtcgcc
     9181 aatcatgctg atggcggaaa cgcccatccc cagcagatcg gtatcgccct gagtggtgta
     9241 gccctggaag ttacgatgca gcacgccttc acgctgggcc accgccagct cgtcatcagg
     9301 acgggcaaag tgatccatac cgataaactg atagcccgat tgcgtcagga aggcgatggt
     9361 ttcctgcagg atatcgagtt tttgctgcgg actcggcagg tcagcatctt tgattttgcg
     9421 ctgagcagca aaaatggtcg gcagatgcgc gtagttaaaa acgctcagac gatcggggtt
     9481 cagctccgcc acacgtttca gggtaaaggc gaaactctcc ggcgtctgtt tcggcaggcc
     9541 gtaaatcagg tcgatgttgg tggaggtaaa accaatctca cgcgcatggt taagcagtgc
     9601 aaagatgaac tcttcatcct gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc
     9661 ctgcacgccc atgctcaggc gattaaagcc ttcggcgcgt aaatgatcga gtacatccag
     9721 ttcgatttcc cgcggatcga cttcgatcga aatctccgca tcggtattga aatggaagtt
     9781 ttcgcgcagc agcttcatca gacggctgat ttgcgcttta ttcagatacg tcggcgttcc
     9841 gccgccccag tgcaattggc tgacgtgacg cccggcgaac agcggtgcac gatggacgat
     9901 ttcttgctcc agcgcgtcca gatactgatc ggccttgtgc tgctggcgag taacaatctt
     9961 attgcaaccg cagaagtaac aaagcttatg gcagaacggg atatgtacgt agagagataa
    10021 tggacgctca ggatagcgcg ccacggcttg taaaaacgcc tgttcgccga agtcttctga
    10081 aaactccagc gcggtcgggt acgaggtgta tcgtggcccg gaatagttat atttctggat
    10141 cagggccaga tcccagtcga tttgctgtac agacatgctc actccttccg atggcgtctc
    10201 tggcggctac ggcgtaccgg cggatgcccg tttcgggtca tcagccggtt gcgcagccac
    10261 ttttgtcgcc gcgacaaccg ctgtagttta acgaataacc acaccagata acatataacc
    10321 ggaagggtta tcagcagaac gggaaggccc acggcggcaa tccgttagtt gccccgcagc
    10381 agacgcatca tgtcttcttg cttctcgtct tcttcctctt cttcgtcatc gtcataagag
    10441 aggccgagtt tctgcatcaa ctcatcaata cgatccagtt tagcatccac ccaggattgc
    10501 tcttcggcgc tcagggtttc gcctgcttcc agacgttcca gcagcgcatc cagacgctca
    10561 tccgtttcca gtaattccaa ctccgcctgc ggtgaaagca taggtttctc actcttcggt
    10621 ttgtgctgtt tggtgacttt ttcagtcact cccaatggaa taggggtttt actgccaata
    10681 cgtggatctt ttggtgcgtt ctggcctttg ctgcctgacg tggtgtttcc gcccgctgcg
    10741 cggctgcccg gcgcatgacc acgacgtttt ttctgacgct tacggtcacg agcttcctga
    10801 tccagctcct cgcgtgtttt tcgacgcgct tttgcatgac ctttgctgcg tgagtttgaa
    10861 gatgatggtt tcataatggt tcgtctttta gccgttttat tcagtataga tttgcggcgg
    10921 aatctaacag aaagcaagca aagaaaaaag gcgacagatt actctgtcgc cttttttcct
    10981 gactcataac ccttaacggg tcttacaatc cttgcaggcc gccaacacac cctgtttttc
    11041 cattagccgg aacaatccgt gttgattcca tttccgttta attacgtctc cggacgtttg
    11101 tcttcctgac aatcctctgt cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt
    11161 gttcctgtta gcgtcctcgc tctatgggtg ctactttacg cctttgcttt aaacaaacaa
    11221 tcaaccgcca tcagtatttg cacattttca gagttttata taaaagcaat tcactgattt
    11281 atatagctta tttgacatgc atcgcagaat tttctctgct atttcgcata aagactatcg
    11341 gcaatgtctc acaaagcgta tggcttttac ttacaacgct cacgccgaaa aatccgcctt
    11401 ttattatggt tcaggtataa tcgccacaaa gctttgactg acggagacga ccgctttgac
    11461 taatttgaat tatcaacaga cgcattttgt gatgagtgcg cctgatattc gccacctacc
    11521 ttccgatacc ggaattgaag tggcttttgc aggccgttcc aacgcaggta aatccagcgc
    11581 gctgaacacg ctgactaacc agaaaagcct ggctcgtacc tcaaaaaccc cagggcgcac
    11641 ccagcttatc aacctgtttg aagtggctga cggcaagcgt ctggttgact tgcctgggta
    11701 cggttatgcg gaagtcccgg aagagatgaa gcgcaaatgg cagcgtgcgc tcggcgaata
    11761 cctcgaaaaa cgtcagagcc tgcaaggtct ggtggtgcta atggatattc gccatccgct
    11821 gaaagatttg gatcagcaga tgattgagtg ggcggtagac agcaatatcg ccgttctggt
    11881 gctgctgacc aaagcggaca aactggcaag cggcgcacgt aaagcgctat tgaatatggt
    11941 gcgtgaagct gtactggcgt ttaacggtga tgtgcaggtt gaaacgtttt cttcgttgaa
    12001 gaaacaaggc gtagacaagc tgaggcagaa actggatacc tggtttagcg agatgcagcc
    12061 tgtagaagaa acgcaggacg gcgaataatt ttcttgcctt aatgcttgtg ccggatgtgg
    12121 cgtatccggc ccgtaaattc aatagattac aattctaccg ttggcctgat aaacatagcg
    12181 catcaggcat tacggatctt ttctttcgcc caataaaaaa cgccccagtc attactgact
    12241 ggggcggcta aaatattcag ccaaatccga ttacgtgaag taaaaggtct gaaagataga
    12301 acatcttacc tctgtaccct acgcgactaa ctttactctt ttttgttcag ttcagaaagc
    12361 actttttgta attttttttc agttgttgca taggaatttc taatagccat cacaaaacgt
    12421 gacagcttat gttgcttact tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct
    12481 gatcccagtt ttcgccactc cccacttcca ccagcaacgg cacatccaga cgggtacagt
    12541 tttccatcag ttgatgaatc tgcttcgcga cggcatcaac atcatcttta tgaacttcaa
    12601 ataccagttc atcgtgtacc tgcatgatca tacgtacacg cggttgctca gcctgtaacc
    12661 acgcatcaac ggcaatcatc gcccgtttga taatgtcggc ggcggttccc tgcattggcg
    12721 cgttaatggc tgcacgttca gccgctgcac gacgagcacc attgctggat ttgatatccg
    12781 gcagatacag acggcgtccg tccagcgttt caacgtagcc cagctctttc gcctgagcac
    12841 gggtgcgttc catatactcc agcacgccag ggtagcgttc gaagtaaagg tccatgtact
    12901 tctgcgcttc tttacgtgga atgttcaatt gccgcgccag accgaaagca ctcatgccat
    12961 aaatcagacc aaagttgatc gctttcgcgc tacggcgttg ctcgctggtg acggtttcca
    13021 gtggcaaacc aaacacttct gccgccgttg cccggtggat atcttttcct tccgcgaatg
    13081 cggtcagcaa gcctttatca cgcgagagat gcgccataat gcgcagttca atctgcgagt
    13141 agtccgctga gacaatcaca taatcctctg gcgcaataaa cgcctggcgg atacgacgac
    13201 cttcttcgtt acgcaccgga atgttttgca ggttaggatc ggttgacgat aaacgtcccg
    13261 ttgcagttac tgcctggtga taagaggtat gcacacgccc ggttttcggg ttgatcatca
    13321 gcggcagctt gtcggtgtaa gtcgacttca gcttcgccag accacgatac tccagaatca
    13381 cttttggcaa cggatagtcc agcgccagtt cttccagtac ctcttccgac gttgacggcg
    13441 cgccacccgg cgttttcttc agcggtttaa taccctgttt ttcaaacaga atggtttgca
    13501 actgcttggt ggaagaaagg ttaaattctt cacctgcaat ttcatgcgct ttcttttcca
    13561 gctcagccag acgcaaggtg agctcttcag aatgattgtg cagcactttc ggatcgatct
    13621 tcacaccgtt acgttcaatg cgtgaaagca ccggcaccag cggcatttcg atattctcga
    13681 agacgttcaa cggccctttg tgtttctgca gatccggcca cattttcaga tgcaactgca
    13741 aggtgacatc tgcatcttcg gcggcgtaac gtccggcctc ttcgagggca atctggttaa
    13801 aggtcagttg atttttgcct ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca
    13861 accaacgttc cgcgaggctg tccatatcgt gacgcccggc aacgctattg agaatgtagg
    13921 actccagcat ggtatcaaac gcaatcccac gcagttcaat gccgtaattc gccagaatac
    13981 cgcgatcgta tttcaggttt tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt
    14041 ttagcaactc gagtgcacgc tcgtgagaga tttgatcggg cgcatcaaga taatcatgag
    14101 caaccggaat atatgccgct acgcctggct cgatagcaaa agaaagcccg accaggttag
    14161 cagagatgtt atcaaggctg tcggtttcgg tatcaaatgc aaataccggc gctttttcca
    14221 gcttcgcaat ccacgctttc agtgtttctt catcaaggat agtgacgtag ttgtcataag
    14281 aaatcaccgt tgccgtcact tctggcgctt cgtctgcaac actggtttcc tgcggcttcg
    14341 cggctgtttt tgcccctttg gcctgtaacc atttgcccgc ttcgacatca gcagtccagc
    14401 gtttgaactc atactttttg aacagcccca acaactcttc cgctgccggt tgctgcactt
    14461 ccagttgttc acaggtcagc tccagttcaa catcggtttt aatcgtcgcc aactggtatg
    14521 agagataagc aacttctttg ttttgctcga gcttcgctgc cattgttttc gcgccacgga
    14581 agctcaaccc agcaattttt tctggctcgg catacagcgt atccagcccg ccaagacctt
    14641 gcagcaatgc ctgcgcggtt ttttcaccga cgcccggtac gccaggaatg ttatcagagg
    14701 agtcacccat cagcgccagg aagtcgatga tcaattctgg cggcacgccg tacttattca
    14761 ccacctcttc cggtccgagg atggtattcg tcatggtatt gataagcgta atatttggcg
    14821 tcaccagctg cgccatatct ttatcgccag tgctaatcag caccggacgc ccggcttttt
    14881 cggcttcgcg cgccagagta ccgataacgt cgtccgcttc tacgccagaa accgccagca
    14941 gcggcagtcc catcgcttta accatcgcgt gcagaggttc gatttgtgct cgcagatcgt
    15001 ccggcattgg cgggcgatgt gatttgtaat gttcaaacag ttcatcacga aaggtttttc
    15061 ccttggcgtc aaagaccacc gctgcatgcg tcggtttata ttgcatgatc agactgcgca
    15121 gcatgttgag gacaccatac atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg
    15181 gaaacgcgtg atatgcgcga taaagataag atgaaccatc tacaaggata agtggatttt
    15241 gggggatctg aaccataatg tccgtgcctg tttatcagat tatgggtaaa ggatgccaca
    15301 gacaggatga aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt agatctgctg
    15361 gatcgctcgc aaatcgtcat gtggataact ttgtgcataa attatttaca tcgcttttaa
    15421 tttattatca tgctttttac agcatgatgt ttgtctttgt ttttcatgca gttaaaaata
    15481 aatccatccc taataattga gataatggga ttttttctaa tgtggataga tatgaattat
    15541 ttttctcctt aaggatcatc cgttatttgg gtcattttct gctaaaatct gcgccttgcg
    15601 cttctttagt gcactgtcta tggctaacct tttgaataaa ttcattatga cgagaatact
    15661 cgctgcgata acccttttgc tgagtatcgt attgaccatt ttggtcacta ttttttgttc
    15721 cgtcccgatc atcattgccg ggattgtaaa actattgcta cctgtgccag tcatttggcg
    15781 aaaggtttcg cgtttttgtg attttatgat gtattgctgg tgtgaaggtc tggcggtatt
    15841 actgcatctg aacccacacc tgcaatggga agttcacggg ctggaagggt taagtaagaa
    15901 gaactggtat ctgcttattt gtaaccaccg tagttgggca gatattgtcg tactgtgcgt
    15961 gctgtttcgt aagcacattc cgatgaataa atatttcctc aagcaacagc tggcctgggt
    16021 gccatttctt ggcctggcgt gctgggcgct ggatatgccg tttatgaagc gttattcccg
    16081 cgcttattta ttacgtcatc cggagcgacg tggtcaagat gttgaaacca ctcggcgttc
    16141 ttgtgaaaag tttcgcctgc atcccaccac cattgtcaat ttcgttgaag gctcccgttt
    16201 cacgcaagaa aaacatcagc aaacccactc cacttttcaa aacttgttgc caccaaaggc
    16261 ggcaggcatt gcgatggcgc taaatgtact gggtaaacaa ttcgataaac tgcttaatgt
    16321 tacgctgtgt tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact
    16381 gacgcggatt gtcgtacatg tggatttaca gcctattgcc gatgagttac atggcgacta
    16441 catcaatgac aaaagcttta agcgccattt tcagcaatgg ctgaactcat tgtggcagga
    16501 aaaagaccgg cttctgacgt ctctcatgtc atcacagcgt cagaataagt aagttttact
    16561 cgcctctgaa tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac
    16621 ataaattctt cttcgctcat ctcctgtccg ttaaaaacaa ctttacccgg tgtataacgc
    16681 agctgcagcg aggcggtatt gttgtcaatc gtgattaact gaaacatctg acctaatgtc
    16741 atcatcccgc tgatttgttt atcagcctgc ttttgcgctt tttccgcatc cattccttca
    16801 gataaattaa gctgttttgc ggtttctgtg acgacattaa gcggtaactt cacatcaaaa
    16861 ttgagcgatt tgatatcttt gtttgtggat gatgaagatt tggctgggtc ggcaatactg
    16921 atatccagat tggcatttag ttcgccgagt gcgttcttcc atcttaccgg ttgtttaatg
    16981 gtcggctcac ttttttgtaa taacggtaaa tactctttga acaatgcagc attcacttct
    17041 tgcagagcaa cttcatcgtt tgctaactca gggtgtgcag caagctgctt ctgcatcgca
    17101 atgttatact ggatgataaa ttggcgcact gctgagggat caatagattc agcaatcaat
    17161 gaccactcac cactaccgag ttgctgattc ccttttgtta acttatcaag ttcgtaggtc
    17221 agattgacat tgatataatc tttgtcttta acgcgatcca gcctggtttt tgcaacgaaa
    17281 gcatcgattt gggcaacgtc ttccccgtga ttgataatgt taatctgatt aaatttcgac
    17341 tcgctttcac caaccggaaa tttcttttct tctaatcgcg ccagagaatc aatggtaaaa
    17401 ctttttgccg ttactttgtt cattggtgat aattgcagaa caatatcttc gactttgcct
    17461 tcaatatcca ggttagacaa tgaactacca tcaccattaa aagtaaactg gccctcacca
    17521 aaaaaaaatt ttccatactc tgcaggggca acatctaatg tcgtagagaa tgagccacca
    17581 aacgcgaatc gcaatgtcgc ttccacgggc gatttatttt tcgcggcgat aaatagcggt
    17641 tgtgtcagtt cattattcac taagttcact tttgccgctg ccagtgctgg gatgacatta
    17701 ccatgcatta acattgtgat gggcaacgga ccgtgctcaa catccacatc aaatacaact
    17761 ttctggcctg gcttgatatt gagatcgggt gcaccgttat cgaatgtcat ttgcatttga
    17821 aaacgggaat taaaaaagcc tttgtcaaag tttttataac ttaacgtcat atcgtaggca
    17881 tgttcacctt tctttttcgc atcgttaaaa tctttaataa acttttcgat accaggctga
    17941 atttgcgtac ctgtgtacca tgtgccacca ccccagatta cggctaacgc aacaataaca
    18001 cctgtagctg acttacgtat catataaata ttccctgata aaacagcaga actttgaata
    18061 tccacttatg ctgaaataat gtccagcggc aggatgcatt atcagaatgt atttatattt
    18121 attaaataat aaaaaaagcc cgtgaatatt cacgggcttt atgtaattta cattgaatta
    18181 tttttcctcg gacagatatt tcaccgtatc agcatactgc tgaacaaaaa catccatatt
    18241 gctggtatcc ataccctgcg gattcagctg atatttaccg ttaacaaaca tcgccggaac
    18301 accacgcaat tgcacatcag ctgcggcttt ttcctgctga gcgaccagag atttcaccac
    18361 gaagctgttc cacgccgcgt cgtactcttc acctttaata cctgcattga taaatacatc
    18421 gcggatatca gatgctgaac gaatggtctg ggttttctgt acgccttcaa acagcggaac
    18481 agtcactttg tcttccacgc ccagcgccat cgccacagcc catgcctgag tcagatcttt
    18541 gcccaggtca ccacccatga agttgacgtg gtatttagtc atcttcacgc cttccggcag
    18601 ttttttcttc acattatcag aaatatgcag aacttcttca aactgatagc agtgcgggca
    18661 gaagaaagag aaaaactcca gcacttgcgg cgcgccagct accggttttt ccagggtagt
    18721 gtactgttta ccatcttcat actgcgccgc cgatgcgcta aacgctaaaa ctaaaccagc
    18781 cagcgccagc caaatctttt tcatgatcta ctctctccga ttaatacata ggtgttaatt
    18841 gcaaaggggg ttcttgtaga acttttgcct gttctataaa agtcgccgtc tgtcgtagcc
    18901 agtaatcttc cccggttaac cacgggaaat ttttcgggaa cgcgggatca gcccaatgcc
    18961 gcattagcca ggcaagataa taaaccaaac gcatggcgcg taaaggttca atcagtccga
    19021 tttcagcggt gtcgaactcg ctaaattctt cataagcttc aataatagtt tccagttgca
    19081 tccgctgctg ggctttatcg ccattgagca acatccacaa atcctgaata gctggaccat
    19141 tacgtgcatc atccagatca acaaacattg gaccatcgcg ccagagaata ttcccggcgt
    19201 agcagtctcc atgtagccgc agaacggtga aatcttcccg ccagtgtgct gtaacggcgg
    19261 cgatcagctc atctgtcgct ttcagaaatg ccgctttcaa cccggaaggt atcagtgtag
    19321 cgtcctcaaa cagcttgcga ggctcaatga gatattcatt cagaccgatg gtcgggcgat
    19381 gaataaaaag ctgtttgcgc cccgtctggt gcatacgccc taaataacgc ccaaccgctt
    19441 ccatctgatc gatattatca gcttcgaact ggcgaccacc gacgcttgga aaaacagcga
    19501 aataaaatcc ctgatgattc aataaagtct gaccgttaaa ggccacaggt gctgcgaccg
    19561 gaacttcatc atttaccagc tgcaacgcaa attgatgttc ttcgaggatt tgatcggctg
    19621 tccaacgttc agggcgataa aatttgacga caaaacgtcg acgctcttcg tcctgaaatt
    19681 gatagacacg gttttcatag ctgttaagcg gggtaagacc ggaatccacc cggatccctt
    19741 gctcaaacag agcgtccatg atggtatccg ggtgtagtgt ctggaaagta aaagcgctgt
    19801 tgttcatccc atcatccgga aaacattacg aatgatgaag gatatcattt cacgatgatt
    19861 ttggtggcgc cgcttacaag cttttactct ttaattacgc cgcgagcgcg taacagcgcc
    19921 gttttgaagt cttcttcata atctttctgc aaacccggga tcaccgcatc tttcgcggaa
    19981 tcacgcattt tcaggtgata gatcagaatg tcatccgtca aatccgccag ttcgccgtca
    20041 aaacctgact ctttcgccag tttctgcaaa aattgcaaca agttaaagtc tggctctttt
    20101 tgccaggctg gctggaggag ttcaataact tcattcagac gtttacattt catagtaatg
    20161 ctccttactc ttgagacaga cacgttagca gggtcaatcc cacaataaaa gaggcgatat
    20221 cggtgaatct gatgacgacg ataacaggcg ttgtgctggc aggcggtaaa gccagacgaa
    20281 tgggcggcgt agataaagga ttgctggact taaatggcaa accgctgtgg caacatgtcg
    20341 ctgacgcgct taagacgcag ctctctcacg tcgtggttaa tgctaatcgt catcaggaaa
    20401 tctatcaggc aggcggtctg aaagtgattg aagattcact ggcggattac ccaggccctc
    20461 tggcaggaat gctttcagta atgcagcagg aagctggtga gtggtttttg ttttgtccgt
    20521 gcgatacgcc ttacattccc catgatttag ccgcccggct taatcatcag cgcaaagatg
    20581 cgcctgtcgt gtgggtccat gacggtgaac gcgatcaccc gactattgct ctggtaaacc
    20641 gcgctattga gcctttatta ctggaatatc tgcaagcagg agaacgccgg gtaatggcat
    20701 ttatgcgtct ggctggcggt catgcggttg atttcagcga tcataaagat gcatttgtta
    20761 acgtgaatac gccagaggag cttgcccgat ggcaggaaaa acgatgatac cgttactcgc
    20821 ctttgccgcg tggagtggta ccggaaaaac tacgcttttg aaaaaactga tcccggcatt
    20881 atgcgccaga gggatccgtc cggggctgat taagcatacg caccatgata tggatgttga
    20941 taagccaggc aaagatagct atgagctgcg caaggctggc gcggcacaaa ccatcgttgc
    21001 cagccagcaa cgatgggcct tgatgacgga aacaccagac gaagaagagc tggatctaca
    21061 ttttctcgca agtcgaatgg atacctcaaa gctggatttg attctggtcg aagggtttaa
    21121 gcatgaagag atcgcaaaga ttgtgctgtt tcgcgatgga gccggacatc gacctgaaga
    21181 attagtgata gacaggcatg ttattgctgt agccagtgat gtgccgctta atcttgatgt
    21241 cgcattactg gatattaatg atgttgaggg gctggctgat tttgtggtgg agtggatgca
    21301 aaagcagaac ggataagcaa gagtaggctg gatcagacgc
//
LOCUS       DAJTUN010000067        21091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTUN010000067 DAJTUN010000000
VERSION     DAJTUN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.067"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..206
                     /locus_tag="OO470_002319"
     CDS             <1..206
                     /locus_tag="OO470_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR6251545.1"
                     /translation="WSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVC
                     DAVVDRVNDHLPGNIDPWKDYNL"
     gene            218..913
                     /locus_tag="OO470_002320"
     CDS             218..913
                     /locus_tag="OO470_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCR6251546.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNTAAGI"
     gene            924..1898
                     /locus_tag="OO470_002321"
     CDS             924..1898
                     /locus_tag="OO470_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HCR6251547.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     QGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            1902..3239
                     /gene="traO"
                     /locus_tag="OO470_002322"
     CDS             1902..3239
                     /gene="traO"
                     /locus_tag="OO470_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCR6251548.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPPGTVTSQHPRLQETSLATPAARPGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            3236..3949
                     /gene="traP"
                     /locus_tag="OO470_002323"
     CDS             3236..3949
                     /gene="traP"
                     /locus_tag="OO470_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCR6251549.1"
                     /translation="MKNEHEDPGSFGETPAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGSGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            3949..4476
                     /gene="traQ"
                     /locus_tag="OO470_002324"
     CDS             3949..4476
                     /gene="traQ"
                     /locus_tag="OO470_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCR6251550.1"
                     /translation="MSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRAR
                     AEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAAA
                     GAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVFN
                     DRVIDATLASLGIAF"
     gene            4523..4921
                     /locus_tag="OO470_002325"
     CDS             4523..4921
                     /locus_tag="OO470_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCR6251551.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            4978..5229
                     /locus_tag="OO470_002326"
     CDS             4978..5229
                     /locus_tag="OO470_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HCR6251552.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            5144..5962
                     /locus_tag="OO470_002327"
     CDS             5144..5962
                     /locus_tag="OO470_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HCR6251553.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            6257..9301
                     /locus_tag="OO470_002328"
     CDS             6257..9301
                     /locus_tag="OO470_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6251554.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            9301..9921
                     /locus_tag="OO470_002329"
     CDS             9301..9921
                     /locus_tag="OO470_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HCR6251555.1"
                     /translation="MYSIHITPRCRRPARGQVFLTTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            9879..11084
                     /gene="traW"
                     /locus_tag="OO470_002330"
     CDS             9879..11084
                     /gene="traW"
                     /locus_tag="OO470_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HCR6251556.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMGQGSVR"
     gene            11081..11650
                     /gene="traX"
                     /locus_tag="OO470_002331"
     CDS             11081..11650
                     /gene="traX"
                     /locus_tag="OO470_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HCR6251557.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11725..13890
                     /locus_tag="OO470_002332"
     CDS             11725..13890
                     /locus_tag="OO470_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCR6251558.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTPSGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPIDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTATSASEYHASKTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINRDDKDGIKQ"
     gene            13978..14631
                     /gene="excA"
                     /locus_tag="OO470_002333"
     CDS             13978..14631
                     /gene="excA"
                     /locus_tag="OO470_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCR6251559.1"
                     /translation="MNTTKSRYPSWWAYYYLIRTAYFIFGVPFLLLCSLGFTGMMLTE
                     PYIETDIQAYLYTYGSWLLLILPSLWLYMKTRAKKKKVQQVVQQIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            14905..15624
                     /locus_tag="OO470_002334"
                     /pseudo
     CDS             14905..15624
                     /locus_tag="OO470_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
     gene            complement(15681..16604)
                     /locus_tag="OO470_002335"
     CDS             complement(15681..16604)
                     /locus_tag="OO470_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011410900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR6251560.1"
                     /translation="MAKQKFKVTNWRNYNKALINRGSVTFWLDDEAIQAWYEPATHTS
                     RGRPQRYSDLAITTVLVVKRVFRLTLRAAQGFIDSIFAMMGVPLRCPDYTSVSKRAKS
                     VNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGKERRRIWRKLHLAVDASTHEV
                     ICADLSLNNVTDAEAFPGLIRQTHRKIRSAAADGAYDTRRCHDELRRKKISALIPPRK
                     GAGYWSGEYADRNRAVANQRLSGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLR
                     DYDGQVAEALAMVRALNKMTKAGMPESVRIG"
     gene            17100..17555
                     /locus_tag="OO470_002336"
     CDS             17100..17555
                     /locus_tag="OO470_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251561.1"
                     /translation="MPYCFKNKSKKYYPDLLIATVDGRCLLMEVKPTDRMTLSLTRIK
                     SNAGRKWAHTRGWGWLVISDRFSLRQLEEHAISTNTWKLIEAELKTSRILAWREMMEL
                     RTRYEIHRLDLIAYIIQSGAEVDNFYRITPKISNNTSNQRQPNESDCHN"
     gene            complement(17693..17845)
                     /locus_tag="OO470_002337"
     CDS             complement(17693..17845)
                     /locus_tag="OO470_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR6251562.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVFVATL
                     AYEVKR"
     gene            18175..18417
                     /locus_tag="OO470_002338"
     CDS             18175..18417
                     /locus_tag="OO470_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251563.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAEKPGKNIH"
     gene            18708..19076
                     /locus_tag="OO470_002339"
     CDS             18708..19076
                     /locus_tag="OO470_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251564.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            19080..19295
                     /locus_tag="OO470_002340"
     CDS             19080..19295
                     /locus_tag="OO470_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251565.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(19493..20116)
                     /locus_tag="OO470_002341"
     CDS             complement(19493..20116)
                     /locus_tag="OO470_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6251566.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            20276..20743
                     /locus_tag="OO470_002342"
     CDS             20276..20743
                     /locus_tag="OO470_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6251567.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            20893..21069
                     /locus_tag="OO470_002343"
     CDS             20893..21069
                     /locus_tag="OO470_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251568.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
BASE COUNT         5180 a         5932 c         5398 g         4581 t
ORIGIN      
        1 catggtcaca gcgggagaat gacgtcgcct cctccctgca gtcctgcctg tcccgcatca
       61 aggacatcag tatcactctt ccgcagttcc ccagcctgga caagctcctc cgcgacctgg
      121 aaaaccaggt ctgtgatgcc gtcgtggatc gggtgaatga ccatcttccg gggaatatcg
      181 acccctggaa agactacaac ctctgaacag gagcaccatg agcgaagcac aacccacctc
      241 ccctgtctca ccggaagcgg ataacagcgg agatatttat aaccccgtgc tcagaaccat
      301 cgcacagtca gaacaacggg ccgcgctggt acccggtctg gtcagggcgt tgctgtacag
      361 cggcgcagca ctctgcatca gcctggtggc taatggcata cagtactggc atgccaccgg
      421 cgtgacgcgc gaatactttg ccactgacaa tggccgactg gtccgtctgg caccaaccag
      481 ccagccggcc tggagtcaga gcgacgtcat gaactttggc agcatgacgc tgagcgaagc
      541 cttcaatctc gattttgtcc actaccgaaa acaaatcacg acacaggccc cccgcttttc
      601 cgaagccggc tttgccggct atgtgaatgc ccttcaggcc tcgaacattc tcgacactat
      661 taaaaaggag aaatttaacc tcacaaccac tatcggggca ggtaccctgg tcaaccaggg
      721 acagatggag aacggcgtct ggttctggaa attccagtat ccggtcagga tgcgcctggt
      781 cggtcagacc agcacccgcc ccgaacaggc cttcaccttt gaaatcactg tccggcgcgt
      841 tgacccccgc atcaaacccg gcggcatgga aatcagccag atggtttccc gtaacaccgc
      901 agcaggaatc tgagactcat atcatgcaca tacatcatct tattggcgca ctgcttattg
      961 tgtgcggcac tgtcacagct gcagataacc cgggctggaa aaatgcacga agcccgcaga
     1021 ccggcggaaa gaccgcacag gcaaccggga acaccccggc aggcggtccc ccgccagccg
     1081 cacaactccc gggaaacctg ccgcctcccg gcgctgctct tgtacaggaa ggcgcatcgc
     1141 tggattctcc cctgaatgcc agtgaaattc gtgaactccg tgggataatg gccgataacg
     1201 aacgggccat cagtgccccg ctcaccagtg tggtgccccg tatcagttcc ctcacggtga
     1261 atctgtctcc gggggccagc ctgccccttg tccgtaccgc tctgcataac gtcagcgtgg
     1321 tgaccttcac ggacgtcaac ggcagtccgt ggccacagtc ggatccgccc tataacgctg
     1381 cgcccggtca ctttgacgtg cagttcaacg gcaatatggt gtccgtcacc cccctgcgcg
     1441 cctgggctgc cgggaatgtg acggtctatc tcaaaggatt gtctgtaccg gtcatcctga
     1501 atatcaccag tggtgaaacg gacaccacta ccctgagtca ggaaatcgac agtcgtctgg
     1561 atctgcgcat tccccggcag ggacctgcca gcccggtggt gtccataccg tcagaaaaaa
     1621 ttgccctgca tgacgccact ctgcaggcct ttctggacgg tgtgccaccg gatgatcccg
     1681 ccatccgccg cctgaaattc tccggtaacg tcccggacac caccatctgg caacacggcg
     1741 atgatcttct cgtgcgctca cgggccatgc tgcgggatga atttgaacag acgctggcat
     1801 cggctgatgg tacgcggctg tggaaactgc cggtgacccc cttactcacc ttctccgtga
     1861 atggtcagtc cgttcacgtt accccggaac tggagtaaat catgagcgca gaacaagaac
     1921 aggacacagc cgcaaaatcc ggcaaaaagc tgtcagccct gctggttatg gggggcgtga
     1981 ttatcatcgg gggcggatac ctgctgctca cctggctgtc ccccgaaaac gctgcatcgc
     2041 tctcgtcagt caatatcaac agcacaacgt ccggcggcgg acggaacgta atggagacac
     2101 agcactaccg ggatttactg cgggctgaca atgaacgtgg tgctgcagaa gcgttacgta
     2161 ataacgagac gtttatcgcc agtctgcccc ggggactgga taccgatacc ggaaacgatc
     2221 agaaaaggga taatcccccc gatgatcagt caggcacttc cgggcaaaac gcatcccccc
     2281 ctcaggaaga tcagactgcc cgggaaaaac ggcgtgaggc attacagaaa ctgcttgccc
     2341 gtatcaatac acagcatcag gatgcccggc caccggtagt ggcagcagcc atgtggagca
     2401 ccaccggcac tcccggtaca gccccacatg cgtcaccgcc cggtaccgtt acatcacaac
     2461 atccccgtct gcaggagacg agtctggcaa ccccggcagc acgtccgggg atccagctga
     2521 taccggcact gacccgcgta ccggcctaca ttgataccga agtggactct gacaatgcgg
     2581 tctcccgtgt ggtggcctcc gtacctgccg gcccatgggc cggggccgtc ctctacagcc
     2641 aggacgccag actcgcaggt cagggaatgg atatccactt tgaccggatg atgtggcggg
     2701 gcatgtttct gaaggtgaat gcatatgccc tgaatgaacg aacaggaatg tcaaccgtgg
     2761 ccagcgacgt gaaccaccgc tggtttaagc acatcgttct gccgtccgta cttggcgggg
     2821 tgggcaatgt cggaagtctt tacaaagacg ccaacacaca gatccttcag ggaaactacg
     2881 gcagtattac cggcagggtg ggaatgcctg atggagaggc gctggccggc gtcattgcag
     2941 gtggcatggc cgaacggggc agccagctgc tgacccgcca ggcagagagc gaaccttacc
     3001 gtcaggtgac agtatcacgg ggcgaggtcg tctccattct gtttgttgag ccggtcatga
     3061 gcaacgatat cagtacgccg tcctccggca ctgccccttc tgagtccccc tcctccggac
     3121 gtccgctcac ccaggcggag gcagagcacc aggcctctga caggatccgg gcggcggtcc
     3181 tccgtaaaca gaatgaaatg cagcaacgct acggcataca acaggaaagt tatccatgaa
     3241 aaacgaacat gaagatcccg gcagctttgg cgaaacgccg gcaccggcat ccggcaaatc
     3301 cttcctgaaa cgccagtata tgggatacag cctgccggtc tgggccggct gtgccctcat
     3361 catcctggtc gccatctggt acaagtttct gcggacaccc tcagagcccc ctgcagatgc
     3421 aggctttgct gtcacaccgc ccgctgctga gagctgggca gagcccatga ccatgacgga
     3481 gtccgggagc ggaaacaaca tgcccgcacc gccggttacc ccacaggcag acatgcagca
     3541 acttgcccgt gatctgaaag cagagctgga cagacgggat gaggaaatcc ggcgcaccct
     3601 gaccatgctg aaggacagta tcaccaccct cagtaacgcc attcagaagg atgaggcctg
     3661 ggcacaggaa acccgtcagt tgcttgagga gacccgtaag caggtgcata ccctgcagat
     3721 gcagggagca ctgccagccc cgaccgggaa taacagacag cctgcgaaaa ccctgccttc
     3781 acctctgcag ggcatgaaaa tcatgtcggt ggagaaaggc atggcctgga tccgctggca
     3841 gggcagtgcc tgggcagtac gggaaggcga cagtctggga aacatcacca tcacccggat
     3901 tgacccgtcc agccgggcag tcatcacctc tgccggtaca ttacgatgat gagcattgat
     3961 gccctgacag ccatagagaa ctttgccagc ggtattttca gtgcaggcat ggagtttctc
     4021 tttacctggg gagagctgct ggggattatc ggccttatcg gtcatctgat gcgcgcccgg
     4081 gcagaaggac gtcacagtat gggaccggga aaatttattg ccggtatctt catctgcggc
     4141 atgctggtca gtctgccctc aatgatcaat gccggtggta cccagatggg cttcagggca
     4201 gattctttcg cccccatcgc atacgtgcag ccgggctcct ttggtgccgc tgccggtgcc
     4261 gtcaatgcca ttctctcact ggcaaaactg gccggcgtcg gttttgtcat gaacggcata
     4321 tccatctggc gcaaatcact gctggacggg cacaccgccc tgagcgccag tgaatccatc
     4381 agtaaaggga acgtcaagtt tgtggccggc gttctgctgg ttttcaacga ccgggttatc
     4441 gatgccactc ttgcaagcct cggtatcgcc ttctgacccc tgtatttttt cagtaaatca
     4501 atctgttaat aaaggagaca ctatgtcacg acttcgtgcc atggcggcgc gtctgcgcgc
     4561 tgcatctgtt tatgttcagt tatgcctgat ggcctgccgt gaccggctgg tacgcctgtt
     4621 actgctgctt cccgtgatgc tgctgcccca caccgcaatg gcaaataccc tcttcgatat
     4681 gattaaaacc gttggcgatg gtgccagagg ctccagcagt tcgtttgtgg ctatcggtca
     4741 ggttattggc ctggtgctct ttatcggcgg actgcttggc ctcaaacaaa tcggtaaaaa
     4801 acccggtgca gggctggcct gcctgctgtc aattctggtc ggcgtctgcc ttgccgtcgt
     4861 accggaactg atgggcagcg gacagaaaac gctcggtctt tctccggttt caatcagtta
     4921 atccgcaaag gcaccgggaa accggtgccg ccttcacaca atgaggagga ccagaccatg
     4981 tcctgtgagc tacccccttt caccctgctc agaacagagc acggcaacgt gattgcccgc
     5041 gacaaccggg ataatcagga gtttgtgttt gcagagtgtt actccaccgc tatagcagca
     5101 ctgattctgg ctgccgtcac cctgtataca gagagaaaag cgtatgaaaa agaccccgcc
     5161 actcatcgcc ccggtccggg aacgatggct tttcccgctc ccgcaaccgg acacggtatt
     5221 cagcggtgaa gccctgttac caccgccagt gctctcaaca gagggacgct gcccctgctg
     5281 tcgcaggacc gtcacccacc gcttcattct ggaagattca tggccattac agcagatggc
     5341 tgacacctgc cgggatacga tcgtcctgct ggaaaaaaac ctcacccggg tgatgcgcct
     5401 gaaaaaacgt ccggtaccgg aaaacgctga cgaaaaaaag aaacacaccc gaaccctgca
     5461 ggatgcggag cggtccctgg cacaggcccg cctgtcggcc agacgactgg ccctgcgtca
     5521 tgtggaaaag tcgcaaatcg tcacaacgga tgcactcagc gaaaatgaaa gtgacctgct
     5581 gcagccggaa ggcccgccct ttcatctctg tgccttctgc cacgcctggc actgtctgaa
     5641 cggctacgct gccgcacagg gagtgatggt ctggctcccc gatttgcatc cggcctcggt
     5701 ggttgccctc aatgcgcggg cactgcagga gattttcagc gataaccgtc agcgggtcag
     5761 gcagggacgg gcagtcctga atgccctggt acagaaccgt ctggccgttg aggagaaatt
     5821 ccgcacctgg cgtccggcgg attttgctga tgccctgcgc cgctggccgc cggcacagcg
     5881 taaaacgttg agggagaaaa tggacggggt ggcgctcatt ctgctgccgg attcgtttcc
     5941 tgacaaaaag tacgtcatgt gacacacacc ggtgccggtg acgttttgcc ggatatcagt
     6001 tctccgcaga tgcattccgg cactttatta tccggtcgct gactgacgat attccgcctg
     6061 acggagatga aaaagagatt gcccggttca ttcgccgcca gcagtttatt tatccgggtg
     6121 taatcacaca gaaatacgcc cctgcccctc aacatattat ttatcttctc tgctgttttt
     6181 tcatgccaca tcccctgctg tggcatcaaa aaatataaga ccgtgatgat tcatccttac
     6241 cggagtgccc catgccatga atatcaaccg cattattttt gccattgaag actgtatcag
     6301 taccctgtca cggtattccg tttccgcatt cttccccgat tactgtgatc tgcacaccgt
     6361 catcggtctg gaccagcagg accgcgaacg ccgcccctgg ctgaaagccc cctacattgc
     6421 caccacaaaa caggggtatt acctttccgt ctttgaaatc agcggtgccc tgaaagaaat
     6481 ggatgaaaac ccggaccaga cggcccccgg gacactggag tcactgattg cagcactggc
     6541 tgagcgaatg aacacggcct ggaaaaattc cggccacaaa atcagttttg ttttcgaacg
     6601 cgatccggag cgtggaaggg atgaaatcga agccatgctc ttaccccagc gaaaatccat
     6661 cgcacgcacg gggatcctgc ttcaggacgt cctggatgaa aaagtcacca cactcacccc
     6721 ctggcttgtc cgggagcgct gctggctgac cgtctggagc tctgaagaac tgctgagccg
     6781 gaccgatcgc aagaaccatc agacccgtgt caggaaactc gctgaacacg ctccgccagc
     6841 ccgttttgcg caggatccct ggcgctggac actttccgcc ctcaaaatac ggcatgacgc
     6901 cttactggac acgctggagc aggctctgac acatgattct gatggcctgc tgattcgcct
     6961 gatggatatc catgaagtgg gacgggaaat ccgccgccaa ctggagcgca acagtacacc
     7021 ggcagtatgg cagcctcatc tgcccgaaga tgcccgtcct gccggctggc gacagggcgg
     7081 ggatacctct gtattccacg caccgtcgct gaatctgcag ttattcacca cacagcctga
     7141 aacacacggc agcctgattc aggccggtga gctctggcac ggtatggtcg ccattaccct
     7201 gccccctcag aatctgcgga cattcaacca gctggtcagg gatgtgcccc gtgcaatccc
     7261 ctggcgtatc cggatggatc tgatgcccga cggcatgaag gcgctggggg tcaagaaaac
     7321 cctgctgacc tacagcagct ttattcctcc tctccggccc atgtatgact cggtaatgat
     7381 gctgaatgac atcaacaagc atgatccggt ctgtatcatg accattgttg ccaccacatg
     7441 gggaaacagc cgggaagtct gtacccgtaa ccaggctctc ctgaaatctg cgctggaagg
     7501 ctggggggta tgcggcacca ccacaacctt cggtgatccg agacgtgcct gggtgaacac
     7561 ggttctggca gcctcccaca gctccggccc gatccccctt tacccgcccc tttcacacgc
     7621 cctttccctg tttcccctga acagggcagg ctcagtctgg cggggacagg gaaatctgat
     7681 gatgcacacc gaagacggct ctgcatggga ggtggcgctg gcgtcatcac aacagaacaa
     7741 gcacacggag ctgacacccg gcgcacccgg tctgggcaaa tcggtactca tcaacgcact
     7801 gtcggagatc cagatagcct cagcccagaa gaatctccct ttcattgcct atatcgacaa
     7861 gggattcagt gcacagggac tggtacagct tatccgtgac agcctgcctg agcagagaaa
     7921 ggacgaagcc gtggggatca ttctgagtaa tgacccggac cacacccgca acctgtttga
     7981 tgtgatgtac ggggccagaa aacccgtcac accggaaaag aacttcatgg tctccgtcct
     8041 ctgtgcattg tgtgtggata ccggcaccgg tcagccctgt aatcccggcg acacccggca
     8101 aatcatcagc agcctggtgg acctggcctt ccgggaatat ggcgagaata accccagact
     8161 ttaccgcgca ggtacggaac ccctggtgga tctcgccctg gaagagagcg gcatcgctga
     8221 acaacatgat gccggatggt ggaatgccgc cacctggttt gaaatcaggg acatgctgca
     8281 tattgcaggg aatatccccg ccgcccagcg ggcacactac caggccatgc ccctgctggc
     8341 tgaaatgtcc gccctgctgg gacagccgtc catccgggat gtgttcggca ccgttcagcg
     8401 tgataactcg gaagagcggt tgctggacta catccgccgt gccctcgatc agggacacag
     8461 tgactacccg atgatgtccg gctgtacccg gtttatgctc agccccgaca cccgcgtggt
     8521 cgccgttgat ctgaacaatg tggccgggga caagacacct gccggacgtc tgcgcaccgg
     8581 tatcatgtat ctgctggccg ggcagatcgc cggcggggat tttgtcctgc cgcagtatca
     8641 ggaggaaata cggaaaaatc tcgacccacg ctaccatgag gtgattttcc gccgcattga
     8701 acagcttgat caggaggtca agaccaaagt ctatgacgaa ctgcacaatg caaaagggat
     8761 caacttcatc tgggaagccc tggacaccca ggagctggag cagcgtaagt tcgggatacg
     8821 cacggtgctg agcacccagc tgctgcagca ttatcctccg gccgtgctca aatccgccaa
     8881 caccctgtgg ctgctgcgtt accgccctga cgaaattccg ttcctgcggg ataactttgg
     8941 cgtcccggaa gtcacactgc ggcgcttcct gaaaatgccg gaaggggccg caccggacgg
     9001 cagcggtgtc ccggtactgg ctgtgttccg tgtaaaaaac ggcacgctgg cccggatcct
     9061 gaaattcacc ctcggtccac tggagctgtg ggcactgaac tcctctccta aagacagcgc
     9121 cctgcgacgt gcgctgacgc aggaggtggg gtcactgcgg gcacgccaga tcctggccga
     9181 acactttccc cggggttcag ccacctcact gatagaacac cgcgcccgca cacatgacag
     9241 tgaaaacgtc atccatgagc tggcagcaga actcatccgt aagcagggat ataacctctg
     9301 atgtacagca ttcatatcac cccccgctgc cggcgtcctg cccggggaca ggtcttcctg
     9361 acaacactgc gcgtggacgg tgacagtttc agcttcaccc tgccgtccgc ctgcttcaca
     9421 ctgcgaccgt cccccaatcc ccgggtcatt tttgccagtg acagacgggc tgacggggag
     9481 atgagtggta ttcgcctggc cctgcccgga gagacaggca cgctgataac aacggccacc
     9541 tggcaggtta acgtgccgga gcgaccgcct tttgatatac agcatgtcat tcactggctt
     9601 ctgcccgcac cactcactgc gctgatgtct gacaacacct ggctgtggcc ggatgtcccc
     9661 ggcacaccgg cactcacagc cggagactgg ccggtcatgg aaagcgacac cccgacactg
     9721 aaacgcgtga agcgtcgtac cttcacgctc atcgataacc gtaaccgtca gggatggatc
     9781 acgcgccgct tccagtgttt cccgctggct ccgctgccgg aatcactcag tcatcgcgta
     9841 ccagacatcc tgtatccccc gaaaacggag aaaaaagaat gcaaagacaa tctgcaacca
     9901 cactgcttgc actccttgta ggctgtgcgg ttatccctgc cacccatgcc tactctgtga
     9961 atgttgtgtc cagtcagccg gtcacccgac agatcatgcc ggagctggcc accatacagg
    10021 gcatcatgga ggaaatggtc cgtatgcaga cggccaccgg cacagccatc acccagaact
    10081 cagaaaaact ggccaccgtc attgcccagg acgggcaggc cacccgtcag cagatgatat
    10141 tcagtaatga aacacaccgc ctcgaggagg ccaggaaaag cttcagcgtg ccggactcca
    10201 tctgcagtga atccgcgtcc ggcattgcag cagaaagccg ccgggcagca gcgtctgcag
    10261 cggcacgtct gagtcagggg ggaggggtca gcagtaaacc catccgggaa cgtctctccc
    10321 gggctgcgga ctccccggtc cgcgaggcgt atgatagtgc cggcatccac gccggttact
    10381 gcacggaagc ggagtatgcc cgctttggcg gaactgatgt ctgccctgcc gtgggcgatc
    10441 tgccgggcgg agacagccag gtacgatcgc tctatcaggg cgcaggcaca gcagataccc
    10501 cggccgctct cacctgggat cagaaacaga ttgatgccgc gacggcttat atgaaaaaca
    10561 ccgccagacc gtcagcgggt cgtgccccgg ggaaaggaga agtgggcaca cagaccgggc
    10621 gcacgtatgt gggtctgcag aatgaataca acggcatcat cgatgccgcc tctcatccgc
    10681 agctgtcact gattgctgac agtaccccta atgaagccac ccgtggggca ctgactgaag
    10741 ccctgcagtc accgtcagct gcggcatatt ttgaccggac cgcatcatca gaagcccgca
    10801 cccgtggcca catgagccag cgggagtttg aggcctttga agccggcaga cggtatgcca
    10861 acaccgactg gcagcaggat ctgcagggca tggaaggcga taaccttctc cgtgagctgc
    10921 tgcgcaccac cgccctgctg aactggcaga tgaacgatct gaaagagcag attcgtcagg
    10981 gtaacgttat cgccgggcag caacttgcgc tggccgcacg gcagtattac ggacagcgcc
    11041 ttggcgaact gtcacaggcc atgggccagg ggagcgtaag atgaaagacg acaacagcat
    11101 cgacgaaaaa aaaccggccc cccgtctccg ccgcatcggt gatataggga taaatctgtt
    11161 cgcaccactg gccgaaaccc gctggatgct gcgcagctgc cgccatatgc tggaccagca
    11221 taaaacgcgc ctgaagcgcc tcatgcccga ccggcaaccg gagagtccgg tctgtatgac
    11281 ctgggaggaa gcggtcaggg ccagcggact gacggtcgac gaactggacc ggcgtctgcg
    11341 acgtcgcagg atgttctggc ggacaggatt ctggctgatg atgataccgg tacttctgct
    11401 gtgtggtatg gcccttgcgg ccaccgcact gccggtcatc accctgatac gtatcctggt
    11461 ctgcctgtta ctgatgagta ccattgcagc actgtgtgcc tcaaaagccc tgattgcgtc
    11521 ataccgcctc tggcagctga atgaacgtcg ggtcagcgag gacgaacagg gcacattccc
    11581 cgattacctg caggcccggc acggctggaa acaggccaca tggctggcca ttacccaccg
    11641 gcagtactga ctgtccggtc tgcaatttct tttcctcatt ccgggggctg atgcccgggc
    11701 atccccttcg gttccggagt taccgtgaaa atcataccca gagcactgag tgcaggtctg
    11761 gcgctggctg ccgtacctgc acaggctttc gtcacctatc aggacattgt gagtgccgcc
    11821 accaatccgg atgacctgtc gcgccaggca ctggtcacca tttttggtga tgtggtcaca
    11881 aatcccctga cagccagcag caacaccatc attggcaacc tgttcatggt gtttaacgca
    11941 attatcgcca cgatggggat agtctggttt ctgtttattg gtatccgtca tgctgtccgc
    12001 acgggacacc agggcagtgt ctttaacaac ggacgggacg tcgtcggcat actcagtgtc
    12061 gtgagtggat ttttgatgat tgtgcccacc ccttccggct ggagtctggc gcaactggtc
    12121 atgctgtggg gcgcatccat catgggcatc ggttccgcca atatcatggt tcagacagcg
    12181 gcagaaaaca ttgctgaagg ctattcaatg accgtacagc cagtacatgt ctccacccgc
    12241 acggcagcac ggggaattct ggagatggaa ctgtgcaaac acgccatgaa tacgggcctc
    12301 gatgacttta accgtaccgc acgttcatca acgccgccca tgagcgaatc ctcacggaca
    12361 gagaatggca gatataccgt caccgtctca aatggcagcg ccacctgcgg gagtgtgagt
    12421 ctctccgtga aaggaaacgg cagtaacaag cagtccgcgc tggcccattt tttcaatccc
    12481 ttcattgaaa cggaatatca aggggttctg acggcacagc gctccgccat ggataccatg
    12541 ctccgtgacc tggacagcgc ggcgcaggaa tttgtgtcag cgtaccggac ccggcgcgac
    12601 acgggtaacg gcaacctgcc ggatattgaa agccgtattc agaaggcagc caaccggtat
    12661 gaagaaacgg tgcagaaggc gttgccaata gataacgcag aaactgagcg cagggagcag
    12721 ctgaaatcct acctgaccac ctacggctgg gttgccctgg gcgcctggta ccagaccttt
    12781 gccaccgcca accagcgact ggctgaactg gccgaccgtg ctcctactgt gagtggcgtc
    12841 tcgtcactag gcgaagccgg ggatacggaa ttcctgaagg aagtcaaggg ggcttaccag
    12901 gcacagctac agaactcagc gtatactcct gcactgggaa cagccacctc tgccagtgaa
    12961 taccacgcat ctaaaactca agaccctcaa tcagcaatct taaaattgat tgggtttaac
    13021 ctaacaagaa tgactaactg gacagccact tatctgagcg gtacaggtac cacaactaat
    13081 caactaaatc cgttgataaa aatgaaaaac attggcgatt acaccatggt tacgaccgaa
    13141 accatctggg cgggttatac caccgtcagg gtactttcca cggccggcag caactccgtt
    13201 tttggtcgac tggtcaatgc cgtcagcgga ttatctgacg ccgcaaaggc ttttctggaa
    13261 gcatccgcac caccactcta cttcctgctg tttttgctgt tttgtgccgg tttttcgtta
    13321 tccatttacc tgccgtttat cccgtttatc ttctggatga ccggcatcgg caactggata
    13381 gtcagcgtac tgattggatg tgctgctggc cccctgtggg ccgccaccca tctgggaaca
    13441 agtcaggacc ggggaagccg ggccgcttac ggttacatct acctgataga cagcatgatc
    13501 agaccgctgc tgatggtgtt cgggttcttt tttgcatcag tcgccatcgt ggccggcggc
    13561 acaatcctga atgcgctgta tgctgctgca ctgatgaatg tccagataaa ctctgtgacg
    13621 ggcgtgttca gcatggcggg atttttgatg atttatgccc gcgcctgtac cacactggtt
    13681 gcagccgttt ttgcgcttca ggcttatctg cctgaccatg taattaattt tctgggcgga
    13741 cgtgacggtg ttaatatgct ggggaatatg gtttcttcag tcaaagatat tattgccgga
    13801 ggtaatacca atatgcgcag aacacctggc gttcgtgaag aaaaactgaa aaatattaat
    13861 agagatgata aagacggaat aaaacaataa atgaaaatat cgaaaaacca ctaaaacacc
    13921 attgccatat tcacctgaag agattaaaat acattcaggt gaatatggag gtttattatg
    13981 aacacaacaa aaagcagata tccttcctgg tgggcatatt attaccttat ccgcactgct
    14041 tattttatat ttggcgttcc ttttttatta ctatgttctc taggatttac cggtatgatg
    14101 cttacagaac catatataga aacagatatt caagcatatc tatatactta tggcagttgg
    14161 ctcctattaa tactcccatc actctggcta tatatgaaaa ccagagcgaa gaaaaagaaa
    14221 gtccagcagg ttgttcagca gataaaagaa agcagtccct ttgcgccgac cagcgattat
    14281 gagcaactgt cattctcgaa gagttgttat ttcgggatcg acattaaaaa cggcaccatg
    14341 ctgtatgtgc gtatttatcc aaacaatgtg atggatgtaa ttggtctgga tattcacaac
    14401 tttacccgca cggttgctga ggacggcaag ctggaaatcc acaccaccta tgtgagtctg
    14461 ccgatgattc ctcttgagat ctccggcatc tcatcaagga cgctggccaa caccatgcac
    14521 accatggctg cacgtggata tgagtacaag gaacgtttcc cacagatgat ccggtatcgg
    14581 gttaaagagt gggaaaaagt tgccggtgtt cctgttgcag aagtattttg aggtagctat
    14641 caactgttag acgataatcc gtcagccggt tttctctgaa ccggcttttt cttttcaggc
    14701 agaatgcctg tctgccgtct atctcaggac gccgcatgga gtctctatgt tttccccaca
    14761 aattcttagc actgtcttgc ccactgtaga caaaatcaat acaattctgc atatcagtta
    14821 tctataaaag ataacaatat cattataaat aattatcata tccacacact caaggcagta
    14881 cctagaaaac ataaagataa aatcatgtct gaatcccgca gcaattttcc agcacgacat
    14941 ggtactccat ggaatactga agagttacag cgtcttctcg tatccatagc ggagggcgtc
    15001 tcgctaaaaa aacttactca aacacatcaa cgatcgtccg ggggaatacg tggagcaatc
    15061 aaacgcctgc ttccttctga gtttcttgag ttcaacgata taaacacggt gaatgatctc
    15121 agcaattact tcaacaagat tcctcgccaa gaacacaata gtatcatcaa taatttaata
    15181 tcattgagca ctgcaaaaac aattgctcat gataaagttg aaatacaaaa gaatagtttt
    15241 aaacaaaata ccccactccc cctctgccaa cccaatacag attttaaaga ttatatagcc
    15301 gatgaacatg atgtcattat gctaattcat tctgctatcg aaagtttatc taataaacga
    15361 catcaatata ttcttctaat gcgactcggc acagatgatt cacctcattc tctggcagat
    15421 attgccgtca aattaggaat ttcccgtgaa agggtaagac aacttcagga acgagccctt
    15481 ggcttactag ctgctaaatc tcgctatgaa gggacacctg gagcttgcct taaagccctt
    15541 attaactcaa taggtaattc accatatgat atagcagtat ggatttatcg tattgttcat
    15601 cgggatttcg ttacatcctc tgagtggctt tgttgaataa atcagattta aggcgagttt
    15661 ccctgtaaca gctccgactt tcagccgata cgtacacttt ctggcatgcc tgccttcgtc
    15721 attttgttca gcgcacgcac catggccaaa gcctctgcaa cctgaccatc gtagtcacgc
    15781 aacgtcagcg aaccaccgaa caactgcttt accctgtaca ttgccgtttc cgctatcgac
    15841 cgacggttgt aatctgttgt ccatttccac cgtgcattgc ttccgctcag ccgttgattc
    15901 gcaacagcac gattgcggtc tgcatactca ccggaccagt agcccgctcc ctttcgggga
    15961 ggaatgagcg cgctgatttt cttgcgacgc agttcgtcat gacatcgccg cgtatcgtaa
    16021 gcaccgtccg ccgccgctga cctgattttt cggtgggtct gccggataag ccccgggaaa
    16081 gcttcagcgt ccgtgacgtt gttcagcgac aggtccgcgc agatgacttc atgcgtactc
    16141 gcatcaacag ccagatgcag ttttcgccag atacgacggc gctctttgcc gtgctttttg
    16201 actttccact caccttcacc gaagactttc agccctgtgg aatcaatcac cagatgcgca
    16261 atttcacccc gggtgggcgt tttaaaactg acgttaaccg actttgcccg cttgctgaca
    16321 ctggtgtaat ccggacagcg aagaggaacg cccatcatgg caaaaatgga atcaataaag
    16381 ccctgtgcgg ctcgcagggt caggcggaac acacgtttaa cgaccaggac ggtggtgatg
    16441 gcgaggtcag aatagcgttg aggccgtccc cgcgatgtgt gtgtggcggg ctcataccag
    16501 gcctgaatag cctcgtcgtc aagccagaaa gtgacggagc cgcggttgat gagggctttg
    16561 ttgtagttgc gccagttggt gactttgaat ttctgctttg ccatgggacg gccagtgttg
    16621 ccggatgatg cgtgatctga tcctttaact cagcaaaagt tcgatttatt caacaaagcc
    16681 cctctgagtt agccgccaaa ttcatcattt ttgccgcagg cattcctaaa gcaagacgag
    16741 atgaaataaa aaattcgctc tcattaatat cacaattaca aaagcagaag ttacgtgaac
    16801 gaagagcaga attagcacac gctcacgctc aggaaagagc ccgaatcaag aaagaaagag
    16861 ttgagttcat attcagtaaa tggctagaca atacacattg gccaaaatat atcgcacctc
    16921 ctccccccgt agaacagcta tgttcacaga gagcggttaa tgatactgac atatctggat
    16981 tcttttactc tcaaaaatta ggtcgtacag tccagtacga atcagggctt gagtttaaaa
    17041 taatgaatct attagaatgt tgcgatcagg ttcttttcta tcaagaacaa ccattttcta
    17101 ttccatattg ttttaaaaac aaatcaaaaa aatactatcc agatttactc attgcgacag
    17161 ttgatggtcg gtgcctactt atggaagtga agccaacaga ccgaatgacc ttgagtctca
    17221 cgagaataaa aagtaatgca ggtcgcaaat gggcacatac tcgaggatgg ggatggttgg
    17281 tcattagtga taggttctcg ttacgtcagc tggaagaaca tgccatctca acaaacacat
    17341 ggaaacttat agaggctgaa ctgaaaacat caaggatttt agcatggcgt gaaatgatgg
    17401 agttacgtac acgatacgag attcatcgtt tagatcttat tgcatatatc attcagtctg
    17461 gtgctgaagt ggataacttt tatcgaatta ccccgaaaat atctaataat acatctaacc
    17521 agagacaacc aaatgagagc gactgtcata attgatgacg cacaggacgg aaaaaataat
    17581 agctgccgga cagcagcatc cagcagtcgt ccggtaaaaa agacaaacaa ggcgcattga
    17641 ggtcagcctt cgcaacaatc cggcggcaga tgtcctgccg ccgtgttgcc cgttaacgtt
    17701 taacttcgta ggctaacgtt gccacaaaca ccgtatttcc ctgctcgata cgaaatccgc
    17761 aaagcgaatc cctcaccatc cagacaaaac acagtatcgt cacacagacg acgattaaca
    17821 tcattaaaaa cgttcgttgt ggcatcgcag cccctccatt gcctttcggc cgtcatcggg
    17881 ctactataat gttgtctagg cattacagtg ggccctgggt tgattgataa aaaaatcgcc
    17941 tggggctttc tgctacatgc cgtccacaaa acgctttcgg catacagcct caagcgcccg
    18001 ccgctattct aatcgttttc ctgtccgcca caatcctttt catgccacca cgtattttgt
    18061 gtcagggaca gaaataaagc cggaaagtga ctacgtgtgc acacatctgt tttccgggct
    18121 gcaggcatcg tgttattttc tgaaatgcaa aataataaaa aacaaggaca catcatgaag
    18181 gatgaaatac acagtaccag cattaacccg gatgttgttg agcagtttct ggcccttatg
    18241 aatacacatt gtaaatcctc agaagaggcg ctcaccatgc tgagtacgct ttgttacaac
    18301 ctgatgatga catgcggtat ccattccatg gacagccaga tgctcaatgg gcatgggata
    18361 tccatgcaac ttcaggggat tgatattgcc gaaaagccag gcaagaatat tcactaacct
    18421 gaacagggcc tgaaaacgct gtcttttcag gcctctgcag gcaaaaactg atgacaacaa
    18481 aatttctgcg tttgtacccc accgatgaaa aagacaaaaa ctcacaccgg gcttctcatc
    18541 attaaagata aaacccgacg tgtcagcctc tacgaaacgc caacggcatg gtgtatcagg
    18601 gggcaggagt gctacagcaa aagcactgga cgccgctgtg gcagccacga cagtctgagc
    18661 agactgcgcc tggacagcat taagcctgtt gaatgagtgg tggcaccatg aaagataaca
    18721 acacaaccgc caccccagaa acctggaaaa tgcgttttct ggccaacggg acatccggca
    18781 catggtttta cactgacgac attgcagtca ctgacgcgtg gcttcgcgcg cacccggatg
    18841 atgtggaggt tatccctctc tgccggatac agccccgcca gcattcccat gaacaacata
    18901 ttgaagaact ccgcgcagcc ctggcccgga tgctccatat gcatcatatg atgctggaaa
    18961 ccacacatta ccgggaaaat atcaccgatg cccgttgtat ccgtgaaatg catgaagcgc
    19021 cattacaggc gctcaatatt ctgtccagat acccgcataa aaaggacgag gtgtgacgca
    19081 tgtggctact gattttctgg ctttcatcgc cctttaatgt ctcttccggt gatgcccgtc
    19141 agcccacact ggcggtgcac agccaggcct ttcataccga acgggcctgc cgcgatgcat
    19201 tgcgcgccat taaaaaggag aatgttggcg aattttctct gcggggcgtt tgtgtttctg
    19261 acggaaatac gaaggaggag gctgagagct catgacggat attactgaca gacatacact
    19321 tcgccgtatg tcctggtcgg aactcatcac ggcaggacag gaagcagaat ttcagcatga
    19381 ttacgagcgt gccagaatag tgtggtcatt cgccttacat gttgcaacaa ccacgattaa
    19441 caaaaatctg gccatagctc atattcgacg ctgcgatact ttgttgcata aatcaaaggc
    19501 tgtacccgga aacaacccgt gtggttccgg tcagcgagtc tgagacctca acatcccggg
    19561 tgatgattgt cattccggac tgacgcagcc gctgcacatc tgcggcaata cgttgaatac
    19621 caagccagaa cagaccatcc agcgcggtga caattaaccc gctttccagc gccagtttca
    19681 gccgtgctgt cggggcttta acctgcccgg ctatatcagt gaaacaacgc gtcgtcatgc
    19741 catagctgtc cgcccgccgt attcgcgtcg caagactgta actgaagcta tccggcacgc
    19801 cggataagtc attctgcaga ctgtatacac atccccgctg ccgctcattg aatacaactt
    19861 ttttctggct gagctgccat tcccgccgca cgctgtcaat catctgcggt gtccgggtta
    19921 acagcagacg aaacggcagt tcaaaactgg tcacataatc aacattaagt aaggcaatcc
    19981 gcagccgctc cctggctcct gctccggtat cacggcattc acgcatgaca tcttcccact
    20041 ggcgcatcag tcgcggtgac tcattcctgg ccctgaactg atatttctca atctgataat
    20101 caatatgttc ctgcataccg tcaccggata tctgttgccg tatcatcacg gatatcagcg
    20161 tactggaatt catgccctgc gtgaagtatc acactgccaa aaaaaacaca cctgaacgtt
    20221 attatttcgc aatattaatt gcacctgttt tcataaataa aaaggagacg atgaaatgat
    20281 aaaatatata ttttcactca ccattatttt ttccgcctct ctctgtgccg cacagattca
    20341 ggggcacgtc atccgcgttc ttgatggtga tacgctggaa atattacagg acaaaaagcc
    20401 tgtcagggtc cgtctggcta atattgatgc accggaaaag aaacagccct ttggacgctg
    20461 gtcagccact cagctgaagg acctggtggc cggaaagccc gtaaccgtgc aatacgcgca
    20521 gacagacagg tatggccgta ttctcggccg ggtctatacc gctgacggca cagaagcaaa
    20581 tcgctggcag gtccgtaacg gcgctgcctg ggtgtacccg gactacaata atgactatac
    20641 tctcccgccg ctccagcgtg atgcccgcgc cctgaagcgg ggattatggg cagataaaaa
    20701 tccggtacca ccatggacct ggagaaaaca gaataagaaa taattatttg ctgatatgaa
    20761 gaaaaaaacc atttcaaaga aaataaatgg caaaaataca gcatggaaaa aattgccatt
    20821 tttaccgccg cgttattttt aatatttgct gtctgctggt cgtaataagc agaataacag
    20881 aaaggtgtaa aaatgaacct ggttgatgca tttgtcacta aagtgatatc cggaccgtat
    20941 gaagaatacg gaaaatggtg ggtggatgta gaatatattt cctggggagt tccgggtaaa
    21001 agccggctca tgtttgactc acgggaacag gcacaggaag tgcaggaagg ttttcgattt
    21061 ttaacctgag catattttgt taaagggcag a
//
LOCUS       DAJTUN010000068        20818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTUN010000068 DAJTUN010000000
VERSION     DAJTUN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.068"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..572
                     /locus_tag="OO470_002344"
     CDS             <1..572
                     /locus_tag="OO470_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR6251569.1"
                     /translation="KRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRK
                     LYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMR
                     RYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYI
                     EYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            674..826
                     /gene="hokA"
                     /locus_tag="OO470_002345"
     CDS             674..826
                     /gene="hokA"
                     /locus_tag="OO470_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6251570.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1013..1225)
                     /gene="cspA"
                     /locus_tag="OO470_002346"
     CDS             complement(1013..1225)
                     /gene="cspA"
                     /locus_tag="OO470_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6251571.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1506..1796)
                     /locus_tag="OO470_002347"
                     /pseudo
     CDS             complement(1506..1796)
                     /locus_tag="OO470_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2230..2940
                     /locus_tag="OO470_002348"
     CDS             2230..2940
                     /locus_tag="OO470_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6251572.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2990..3964)
                     /gene="ghrB"
                     /locus_tag="OO470_002349"
     CDS             complement(2990..3964)
                     /gene="ghrB"
                     /locus_tag="OO470_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6251573.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4068..4718)
                     /locus_tag="OO470_002350"
     CDS             complement(4068..4718)
                     /locus_tag="OO470_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6251574.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4881..7214
                     /gene="bisC"
                     /locus_tag="OO470_002351"
     CDS             4881..7214
                     /gene="bisC"
                     /locus_tag="OO470_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6251575.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7183..7623)
                     /locus_tag="OO470_002352"
     CDS             complement(7183..7623)
                     /locus_tag="OO470_002352"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6251576.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7620..8183)
                     /gene="tag"
                     /locus_tag="OO470_002353"
     CDS             complement(7620..8183)
                     /gene="tag"
                     /locus_tag="OO470_002353"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6251577.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8341..9039
                     /locus_tag="OO470_002354"
     CDS             8341..9039
                     /locus_tag="OO470_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6251578.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9268..10476
                     /locus_tag="OO470_002355"
     CDS             9268..10476
                     /locus_tag="OO470_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251579.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10800..12491
                     /gene="eptB"
                     /locus_tag="OO470_002356"
     CDS             10800..12491
                     /gene="eptB"
                     /locus_tag="OO470_002356"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6251580.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12583..12659
                     /locus_tag="OO470_002357"
     tRNA            12583..12659
                     /locus_tag="OO470_002357"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12617..12619,aa:Pro,seq:cgg)
     gene            13403..15010
                     /gene="dppA"
                     /locus_tag="OO470_002358"
     CDS             13403..15010
                     /gene="dppA"
                     /locus_tag="OO470_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6251581.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15318..16337
                     /gene="dppB"
                     /locus_tag="OO470_002359"
     CDS             15318..16337
                     /gene="dppB"
                     /locus_tag="OO470_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6251582.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16347..17249
                     /gene="dppC"
                     /locus_tag="OO470_002360"
     CDS             16347..17249
                     /gene="dppC"
                     /locus_tag="OO470_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6251583.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17260..18243
                     /gene="dppD"
                     /locus_tag="OO470_002361"
     CDS             17260..18243
                     /gene="dppD"
                     /locus_tag="OO470_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6251584.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18240..19244
                     /gene="dppF"
                     /locus_tag="OO470_002362"
     CDS             18240..19244
                     /gene="dppF"
                     /locus_tag="OO470_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6251585.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19274..20545)
                     /locus_tag="OO470_002363"
     CDS             complement(19274..20545)
                     /locus_tag="OO470_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6251586.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5031 a         5119 c         5442 g         5226 t
ORIGIN      
        1 tcaagcgata ccgctcttac agaggagagg tagggcaaac cgcccctaat gttctccaaa
       61 gagatttcaa ggctacgcgg ccaaacgaga agtgggttac cgatgttact gaatttgcag
      121 tcaatgggcg caagctgtat ttgtctccag taatagatct cttcaacaac gaagttattt
      181 cttacagcct ttcggaaaga ccagtgatga acatggttga gaatatgctc gatcaggcat
      241 tcaaaaagct taatcctcac gagcatcctg ttctgcactc tgaccaggga tggcagtatc
      301 gtatgagaag atatcaaaat atccttaaag aacatggtat taaacaaagc atgtccagaa
      361 aaggcaattg tctggataat gctgtggtgg agtgtttctt tggaacctta aagtcggagt
      421 gtttttatct tgatgagttc agtaatataa gcgaactgaa ggatgctgtt acggaatata
      481 ttgaatacta taacagcaga agaattagcc tgaaattaaa aggtctgact ccaattgaat
      541 atcggaatca gacctatatg cctcgtgttt aactgtccaa ctttttgggg tcagtacaaa
      601 gtgaggctac cctatgcctg aacaccataa ggttagcctc ttactcgttg gaaatcaaca
      661 cagggggctg ggaatgccgc agaaatatag attactttct ttaatagtga tttgtttcac
      721 gcttttattt ttcacctgga tgataagaga ttcactgtgt gaattgcata ttaaacagga
      781 gagttatgag ctggcggcgt ttttagccta caaattgaaa gagtaagagt cttcggcggg
      841 aaattattcc cgccttactt acggcgttgc gcattctcat tgcacccata tttattcttc
      901 acaaaaataa taatagattt tattacgcga tcgattattt atttcctgaa aaaaaataaa
      961 aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga gattacaggc
     1021 tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag gacactttct
     1081 gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt acgaacacat
     1141 ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg aaccatttta
     1201 cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt gtgcctttcg
     1261 gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta cgaggggtat
     1321 caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct gtacgtcaaa
     1381 ccgttgatgt gcattaagcc actcattggc gggtgatgca acaattattt ttcatattta
     1441 tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat aaagtggata
     1501 aaagtctatt tcatcaactg cttacttaat gccgggttgg cttgaatcaa acgcatcaat
     1561 tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac catggcgact
     1621 gatacgccca aaacccgggc gaaatcatcg atttttaatc ctgtcccttt tcataactgc
     1681 tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta
     1741 aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata ttccattgaa
     1801 aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa gaatagtcgg
     1861 gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc gattcagcct
     1921 gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat atctcttatc
     1981 actcctgttt tgtaaagatt tcgcaaatct aaccttctgc gaaggcgtaa atctctggct
     2041 acaaaaaagg caacggcact atcatttttt aacaatgaaa caacaaatta accttgtttc
     2101 attttctcca tgattttacc aggaagatta cgtagaaagg gtatcctgca agcctgtcct
     2161 ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg
     2221 agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta
     2281 ttaatggtag ttagcctgag tgggtgtttt gataaagaag gcgatcagcg taaagcgttt
     2341 atcgacttcc tgcaaaatac agtgatgcgt agcggtgaac gtctaccaac cctgactgcc
     2401 gatcagaaaa aacagtttgg tccttttgtc tctgattacg cgattctgta tggttattct
     2461 cagcaggtga atcaggcgat ggattccggt ctgcgcccgg tcgtagacag cgttaacgca
     2521 attcgcgtac cgcaggatta cgttacgcaa agcggtccgc tgcgtgaaat gaatggttcg
     2581 ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc aggccgatgc tgcgcactct
     2641 gcgctaaaac agagcgatga cctgaaaccg gtatttgatc aggcattcac caaagtggta
     2701 acgacgccag ctgatgcatt gcaaccgttg attccggcgg cacaaacttt cacgcaacaa
     2761 ctggtgatgg ttggagacta cattgctcag caaggtactc aggtgagctt tgtcgcaaat
     2821 ggcatccagt tcccgacttc acagcaggca agtgaatata acaaactgat tgcgccatta
     2881 ccggcacaac atcaggcgtt taatcaggcc tggactacag cagttactgc aacccaataa
     2941 agagtaaaag cccgagcgat tatcgggctt tacttcacgc agtcgcagct tagtccgcga
     3001 cgtgaggatt cacacagttc ttctcaacct ttccttgtaa cgcatcaatc aaattatcca
     3061 cggcacaggc ggccatgcca taacgcgtct catgggtggc agatccaata tgcggtactg
     3121 cgacgacgtt ggccattgag agcaacggcg aatctacgga cagtggctct tgttcgaaga
     3181 catccagccc ggcggcgtga atttcgcctt tctgcaatgc tgcgatcagt gcattttcgt
     3241 caaccaccgg gccacgtccg gcattaatga aaatggcgga ggatttcatt ttggcgaatt
     3301 gttctgcgcc aaacagatga tgcgtctcat cagttaacgg caggatcagg caaacgaaat
     3361 ctgactcttg taacagagta tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt
     3421 ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt gaagccaaag tgcacacgtt
     3481 gtgccagcgc catgccgatc cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt
     3541 cagtgccgta ccagtccggg cctatgctcg cggtccattc gcctgctttt acccgttctg
     3601 ctacctccac aacccgacga gcggtagaca acaccagcgc catcagcgta tcggcgacgg
     3661 tttctgttaa tacggttggc gtgtgcatca gcagaatttt tcgggcggta agcgcatcga
     3721 catcaaaatt gtcatagccg acggagatcg ttgatgtggc acgcagtttc ggcatttttt
     3781 ccagcaatgc ggcatctaca ttctcgtttg aacccagtaa accttcagct tctgcaaaaa
     3841 ttgctgcatt ttgaacgacg gtttgtgggc tgaggtttgc cacctggtga acggtgaaat
     3901 gcgcttgcag gcgttgcagt aaatcatcag gtaaggcttt gtagaggata acggacggct
     3961 tcatgcttct ctccattcac tgataaggcg atcaccttag cctgaaatat gatgaaggaa
     4021 aactgaagaa tgccaggtga tttctgcatc acctggcatg aaagggatta cagcgggctt
     4081 aaggtaattt ctacacggcg gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt
     4141 gggttagccg ggccaaggcc ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc
     4201 aacgcgctgg caacggaatc cgcacgctgc tgggagagac gcatgttcag gtcgtgaccc
     4261 cccgtgctgt cggtataacc aatcacgtta accgccgttt tcggatactc tttcagcacc
     4321 attgccacgc cggtcagggt gttagcgcct gccggtttca ggggagcgct gctgctgtcg
     4381 aaggtcacat tgttcggcat attgaggata atgttatccc cgctgcgggt tacgctaaca
     4441 ccagtgccgc gcattttgtc acgcagcttc gcttcctgca catccatgta ataacccacg
     4501 ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc
     4561 ttcgaagaag agagcgcacc aatacccgcg cccacgagag agcccagacc tgcgccgata
     4621 gcagatttgc ctgcttcgcg ttcgccggtg taagggttag ttgtgcagcc agataccgcc
     4681 agagcgccac tcactacggc ggcaagtaag ataaacacgt ttcttcattg ttaatcctta
     4741 ataacctttt tattctttgt cacgggttcc gtggcgggag attatgccgc gtgaacatga
     4801 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
     4861 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
     4921 cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc tcgcgtggcg
     4981 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
     5041 atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg gaaaacccgc
     5101 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggatctta
     5161 ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
     5221 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
     5281 tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt gcggcacagg
     5341 cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc agttggccgc
     5401 tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc aatacgctga
     5461 aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca ctgcgtgaca
     5521 gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat ttctttggcg
     5581 ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg ctggggatcg
     5641 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt tgcaccacag
     5701 gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg aaaaacgccg
     5761 aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt
     5821 tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa cagtttggtg
     5881 agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc ggcacacccg
     5941 gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg cggcgttctg
     6001 cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg gataaaatcc
     6061 ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa cacaacggta
     6121 tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc aactttactc
     6181 atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg gtggtgatct
     6241 ctgaatgctt ctggacggcg gcggcgaaac acgcggatat tgttctgcct gcgactacct
     6301 cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat ctggtgccga
     6361 tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt
     6421 tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc
     6481 aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag caggttgaat
     6541 tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg gaaaacccgg
     6601 acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg catccgttaa
     6661 aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac ggttacccgg
     6721 attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat gccgaaccag
     6781 aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag ctgaattaca
     6841 gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt catcctgacg
     6901 atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac tcccgtgggc
     6961 agatccttgc cggagcggtc attagcgagg gaattaaacc tggcgtgatt tgcattcacg
     7021 aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga
     7081 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacga
     7141 cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg tttgaaccac
     7201 cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
     7261 gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat aaacctccag
     7321 catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa taccgcgtct
     7381 gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa tgctgacaaa
     7441 accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa gataggcatc
     7501 ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat gcccccaggt
     7561 tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg cttcccgaat
     7621 catggtttat ttcccagata gcaacagcag ccaaccacat gatcattcac cagcccacat
     7681 gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccacg ttttttcagt
     7741 gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct
     7801 tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa cggttcgccg
     7861 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttccctcga
     7921 tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg cattgctgcg
     7981 accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg
     8041 gtgatccacg ataacccagc ctgctgccct tcaaagcaga tcatttcgaa cagtttttta
     8101 ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag cgggccctga
     8161 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
     8221 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
     8281 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
     8341 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc
     8401 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt tgatacgcaa
     8461 cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga ttacaacgat
     8521 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
     8581 gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
     8641 gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac cacctcgatg
     8701 tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca tgccagcgtg
     8761 agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc
     8821 agctataatc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
     8881 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
     8941 aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac taataatagc
     9001 gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca caattgaaac
     9061 cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa taaatttggt
     9121 gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc tgatggcatt
     9181 tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc agagattttt
     9241 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tacccgctgg
     9301 ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta tacctggagc
     9361 ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt cgctttctct
     9421 ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa attgcaggaa
     9481 cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt aggcttcttc
     9541 ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt
     9601 ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg gttcccggag
     9661 cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag cctgggtttc
     9721 aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt tgtgatttgg
     9781 ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa
     9841 caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc agagtcgatg
     9901 cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat gagcggcctg
     9961 tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga tgtggtttcc
    10021 gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct ggtgctgggc
    10081 attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt gatttcgctg
    10141 gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt
    10201 gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact ggtcagtgag
    10261 ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc
    10321 ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt gaccttctac
    10381 gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg tcagccagag
    10441 cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag ctactgggct
    10501 tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact cgtaaaattt
    10561 gggccagtta acaggccctt tttactgctt tttgtaacgc taaagtctct tttcaaactt
    10621 gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc gctttgctgt
    10681 ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac ttttcccctg
    10741 ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg tttgtttgca
    10801 tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca atctatattg
    10861 gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg cacgatttta
    10921 ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg gtgaccttct
    10981 ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg
    11041 tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg gtcattggtt
    11101 atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa gttgttggtc
    11161 tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt atctggaata
    11221 accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt attcgcagcc
    11281 tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg ctggatatcc
    11341 agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag
    11401 cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg
    11461 tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt acataccagg
    11521 ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc acgcgttggg
    11581 accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc caggagaaaa
    11641 atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca ctgcgttgca
    11701 tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa gaacagaaca
    11761 ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg cagagcgaaa
    11821 tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag attggtgcgg
    11881 agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa atgcagcaat
    11941 cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa ggttcgcatt
    12001 ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg
    12061 ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg gtgacctatg
    12121 tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg attgtgttct
    12181 acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc acgccgcgtg
    12241 aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca gataaatatc
    12301 tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag
    12361 tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc tatacttcac
    12421 cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca aaagtggcag
    12481 cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg ctattaaggg
    12541 attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt ggcgcagcct
    12601 ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat caccgaccaa
    12661 attcgaaaag cctgctcaac gagcaggttt ttttgcattt gtagctcatg aggatgagaa
    12721 cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc agcgtcgcat
    12781 caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc gaccaacaca
    12841 ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac ggatttgtga
    12901 cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact tatggctggc
    12961 gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag atatccacct
    13021 tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatgtt gctaaatatt
    13081 aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa atagctgttt
    13141 gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc ggttggctgt
    13201 attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt tatggaggat
    13261 ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg ttgacccgac
    13321 cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt aaaacaacaa
    13381 acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga tgctgaagct
    13441 tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa ctctggttta
    13501 ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg gcaccaccta
    13561 tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg gcaccaccga
    13621 agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa cctatacctt
    13681 ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga cgcgcgaaat
    13741 gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa acccgtacca
    13801 taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg agctgatcag
    13861 cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac cggaagcgcc
    13921 gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat atgctgacgc
    13981 gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa ccggtccgtt
    14041 ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg atggctactg
    14101 gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg cttccgtgcg
    14161 ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc cggcagacat
    14221 cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc tgaacgtcgg
    14281 ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc gccaagcact
    14341 aacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg cgggtgtatc
    14401 agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc aggattacac
    14461 ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa aaggtttctc
    14521 catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc gccgcatggc
    14581 ggagatgatt caggcagact gggcgaaagt gggcgtgcag gccaaaatcg tcacctacga
    14641 atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa tgatgggctg
    14701 gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct gcgccgcctc
    14761 tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc tgattcaacc
    14821 ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag cgcaggtggt
    14881 gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg aaccggtacg
    14941 caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg aaaacgtctc
    15001 tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc tgatgtgctc
    15061 cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt tgtaggccgg
    15121 ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca atctgtatac
    15181 ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca ttctctgtcc
    15241 gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc tgcgagtcac
    15301 tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc gtcatcccca
    15361 cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg ggcgatccgg
    15421 tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag ctgctggctg
    15481 aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg ggcgtaatgc
    15541 acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag ttcgtgccgc
    15601 gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg gcagtcggta
    15661 ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac actgcagttg
    15721 gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg ctgatcatgc
    15781 tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat atggtgttcc
    15841 tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc tggggtgaag
    15901 acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg ctgggtacta
    15961 ttccgctagc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg ctgggcgagg
    16021 attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt atcgtccatg
    16081 cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg ggaacattgc
    16141 tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg cgctggttga
    16201 ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg ctggtggcga
    16261 cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg aacccgcgta
    16321 ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa gtgattagcg
    16381 caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc aacaaaggcg
    16441 cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt gccaactgga
    16501 ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg ccagcctggc
    16561 aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt gatgtgctgt
    16621 cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg gtggtgttgt
    16681 cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc ctggttgata
    16741 acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagcctgctg ttggcactgg
    16801 tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg accttcgttg
    16861 ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg aatcgcgatt
    16921 acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt attaacatct
    16981 tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct aacgccattc
    17041 tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg cctgagtggg
    17101 gcactatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc gtgaccttcc
    17161 ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac ggtctgcgtg
    17221 acgcgctcga tcccaaacta aagcagtaag aggttcgaga tggcgttatt aaatgtagat
    17281 aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga ccgcatcagc
    17341 tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc cggtaagtcg
    17401 gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat ggcagaaaaa
    17461 ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg caacctggtg
    17521 ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc gtgctacacc
    17581 gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa aagtacccgc
    17641 cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc ttcgcgtctg
    17701 gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat cgccatggcg
    17761 attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct ggacgtgacc
    17821 attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa catggcgctg
    17881 gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat cattgtgatg
    17941 tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc gccgcgtcac
    18001 ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa agaacgtctg
    18061 gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg cctgcttaac
    18121 ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct gaatatgctc
    18181 gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag gcccacacta
    18241 tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac ctgaaaaaac
    18301 attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg ctggacggcg
    18361 tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct ggctgcggta
    18421 aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc gagctgtatt
    18481 atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt cggcagaaaa
    18541 tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa gttgggcaaa
    18601 ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt cgggaaaaag
    18661 ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc tatccgcaca
    18721 tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg ctcgacccgg
    18781 atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt gcgcaggtgc
    18841 tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt atctcccacg
    18901 acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta ggccgctgcg
    18961 tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac acccaggcgc
    19021 tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc aaactcaccg
    19081 gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc cgctgtcgtc
    19141 ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga ggtcaactgg
    19201 tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca aaaccggggc
    19261 catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc aaaatgccgt
    19321 aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg actttataga
    19381 tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc aactgaaaga
    19441 acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg ataaaagcgc
    19501 agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt cggcttaaca
    19561 gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca aagaatgcgg
    19621 tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt ccagggatca
    19681 cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg gcttcttcgt
    19741 ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg agcgtgatat
    19801 aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct gcttcccgtt
    19861 tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa aagaagcaaa
    19921 atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag gcggttatat
    19981 tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc cctactttga
    20041 ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac gcaatcgcta
    20101 tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg gcatcggtta
    20161 aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag tagataaaaa
    20221 tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag ttcttcccca
    20281 ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa agggttttta
    20341 aataaatgtc ctgcactacc caggtgacag gataagcaat gatcgctgcg gtaataaata
    20401 cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca ccaatcgcca
    20461 tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat ttctgattgt
    20521 gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg cctcgcaaaa
    20581 atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc agtatgtaaa
    20641 aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac aaaattgcgc
    20701 atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca aataaagaaa
    20761 ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt aaaaagca
//
LOCUS       DAJTUN010000069        20503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTUN010000069 DAJTUN010000000
VERSION     DAJTUN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.069"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..828
                     /locus_tag="OO470_002364"
     CDS             <1..828
                     /locus_tag="OO470_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6251587.1"
                     /translation="NRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFI
                     IDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPK
                     YKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFK
                     TSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVL
                     GLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1194..2261
                     /locus_tag="OO470_002365"
     CDS             1194..2261
                     /locus_tag="OO470_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6251588.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2258..4993
                     /gene="rbbA"
                     /locus_tag="OO470_002366"
     CDS             2258..4993
                     /gene="rbbA"
                     /locus_tag="OO470_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6251589.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4993..6117
                     /locus_tag="OO470_002367"
     CDS             4993..6117
                     /locus_tag="OO470_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6251590.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6176..6451
                     /locus_tag="OO470_002368"
     CDS             6176..6451
                     /locus_tag="OO470_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6251591.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            6448..6807
                     /locus_tag="OO470_002369"
     CDS             6448..6807
                     /locus_tag="OO470_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6251592.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6927..7328)
                     /gene="nikR"
                     /locus_tag="OO470_002370"
     CDS             complement(6927..7328)
                     /gene="nikR"
                     /locus_tag="OO470_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6251593.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7334..8140)
                     /gene="nikE"
                     /locus_tag="OO470_002371"
     CDS             complement(7334..8140)
                     /gene="nikE"
                     /locus_tag="OO470_002371"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6251594.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(8137..8901)
                     /gene="nikD"
                     /locus_tag="OO470_002372"
     CDS             complement(8137..8901)
                     /gene="nikD"
                     /locus_tag="OO470_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6251595.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8901..9734)
                     /gene="nikC"
                     /locus_tag="OO470_002373"
     CDS             complement(8901..9734)
                     /gene="nikC"
                     /locus_tag="OO470_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6251596.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9731..10675)
                     /gene="nikB"
                     /locus_tag="OO470_002374"
     CDS             complement(9731..10675)
                     /gene="nikB"
                     /locus_tag="OO470_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6251597.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10675..12249)
                     /gene="nikA"
                     /locus_tag="OO470_002375"
     CDS             complement(10675..12249)
                     /gene="nikA"
                     /locus_tag="OO470_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6251598.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12360..12947)
                     /gene="acpT"
                     /locus_tag="OO470_002376"
     CDS             complement(12360..12947)
                     /gene="acpT"
                     /locus_tag="OO470_002376"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6251599.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13002..14051)
                     /locus_tag="OO470_002377"
     CDS             complement(13002..14051)
                     /locus_tag="OO470_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6251600.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14185..15402
                     /locus_tag="OO470_002378"
     CDS             14185..15402
                     /locus_tag="OO470_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251601.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15406..15963)
                     /gene="dcrB"
                     /locus_tag="OO470_002379"
     CDS             complement(15406..15963)
                     /gene="dcrB"
                     /locus_tag="OO470_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6251602.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16036..16701)
                     /locus_tag="OO470_002380"
     CDS             complement(16036..16701)
                     /locus_tag="OO470_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6251603.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16922..17167
                     /gene="tusA"
                     /locus_tag="OO470_002381"
     CDS             16922..17167
                     /gene="tusA"
                     /locus_tag="OO470_002381"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6251604.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17269..19467)
                     /gene="zntA"
                     /locus_tag="OO470_002382"
     CDS             complement(17269..19467)
                     /gene="zntA"
                     /locus_tag="OO470_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6251605.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19541..20167)
                     /locus_tag="OO470_002383"
     CDS             complement(19541..20167)
                     /locus_tag="OO470_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6251606.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20308..>20503
                     /locus_tag="OO470_002384"
     CDS             20308..>20503
                     /locus_tag="OO470_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 family protein"
                     /protein_id="HCR6251607.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTP"
BASE COUNT         4764 a         5411 c         5671 g         4657 t
ORIGIN      
        1 aacagaagag ataatgtttt agaaagtctt cagccttatt tggttattaa taaagacgca
       61 gtcacgccat gtacgtttct tggcgaccaa accggcgatc gatttagtaa tatctgtggc
      121 gatcaattta tcatcgattt gttaaaacgc ataatgagta tcaatgaaaa tgttcatgta
      181 ttagctggaa atcatgaaac gaattgcaat ggtaactata tgcaaaactt cacgcgcatg
      241 aaaccacttg atgaggatac ctacgacggg ataaaagatt atcctgtatg tttctatgac
      301 cccaaataca aaataatggc aaatcatcat gggataactt ttgatgatca gcgaaaacgt
      361 tacattatag gaccgataac tgtctctatt gatgaaatga ccaatgctct cgatccggtc
      421 gaattagcgg aaattattaa taaaaagcat catgctataa ttaatggcaa aaagtttaaa
      481 actagccggg ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac
      541 agacctaaac ttgaagcact attagcatgt tcacaaatgt taggtataaa tcaagttgtt
      601 gcacataacg gaaatggtgg gagagaacgc ataggtgaaa cggggacagt tttaggtctt
      661 aacgcccgtg acagtaaaca tgctggaaga atgttcagca tgcataattg ccaaattaac
      721 cctggtgcag ggccagagat aaccactccc tggaagtctt accagcatga aaaaaacaga
      781 aatggactta tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac
      841 atgaatatta atgttactat ctggttgaga ctgttgataa aacgtaaaaa ggaatgctct
      901 gttcctgcaa ctgaacaggc gtgaaatgcg atccaacagg atggataccg ttttgtcagc
      961 cagttttata gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg
     1021 atgacaacat tactacccgt tttcactaag ccttccccgt tggcgctcaa tgctctgcgc
     1081 gctggtcgta tttgtcgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc
     1141 ggcggcagtt gtaacacgcg tcgtttgctt acagggatta tggcgggtcg attatggata
     1201 agagtaagcg ccatctggcg tggtgggttg tcggggcact ggcggtggct gccgtcgtgg
     1261 cgtggtggct gttgcgcccg gcgggtgtgc cggaaggctt tgctgtcagc aatgggcgca
     1321 ttgaagcgac ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg
     1381 tgaaagaagg ccagtttgtt cgcgaaggcg aagtgctggc gaagatggat actcgcgtgt
     1441 tgcaggaaca gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc gccgttgccg
     1501 ccgcgcaggc tttgctggag caacgacaaa gtgaaactcg tgccgcacag tcgctggtca
     1561 atcaacgcca ggccgaactg gactctgttg ccaaacgtca tacgcgttcc cgctcgctcg
     1621 cccaacgagg ggctatttct gcgcaacagc tggatgacga ccgcgccgcc gctgagagcg
     1681 cccgagctgc gctggaatca gcgaaagccc aggtttcggc ttctaaagcg gctatagaag
     1741 cagcacgcac caatatcatt caggcgcaaa ctcgggtcga agcagcgcaa gccactgaac
     1801 ggcgcattgc cgcagatatc gatgacagtg aactgaaagc cccgcgtgac ggacgcgtgc
     1861 agtatcgggt tgccgagcca ggggaagtgc tggcggcagg cggtcgggtg ctgaatatgg
     1921 tcgatctcag cgacgtctat atgaccttct tcctgccaac cgaacaggcg ggcacgctga
     1981 aactgggcgg tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt attcctgcaa
     2041 ccatcagttt tgtcgccagt gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg
     2101 aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc
     2161 atctggaata tgtcaaaacc ggtttaccgg gcgtagcgtg ggtgcgggtg aatgaagaac
     2221 ttccgtggcc tgacgacctc gtggtgaggt tgccgcaatg acgcatctgg aactggttcc
     2281 cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct
     2341 gaacaatatc actctcgata ttccggcccg ttgcatggtc gggctgattg gtccggatgg
     2401 cgtcgggaag tcgagtttat tgtcactgat ttccggtgcc cgcgtcattg agcagggcaa
     2461 tgtggtggtg ctgggtggcg atatgcacga cccgaagcat cgccgagacg tctgcccgcg
     2521 catcgcctgg atgccgcagg ggctgggcaa aaacctctac cacaccttgt cggtgtatga
     2581 aaatgtcgat tttttcgctc gcctgttcgg tcacgataaa gcggagcggg aagtgcgaat
     2641 taatgagctg ctgaccagca ccgggttagc accgtttcgc gatcgtccgg caggtaaact
     2701 ctccggcggg atgaagcaaa aacttgggct gtgctgcgcg ttaatccacg acccggaact
     2761 gttgatcctt gatgagccaa caacgggggt tgacccgctc tcccgcgccc agttctggga
     2821 tctgatcgac agtattcgcc agcggcagag caatatgagc gtgctggtcg ccaccgccta
     2881 tatggaagag gccgaacgct tcgactggct ggtagcgatg aatgccggag aagtgctggc
     2941 aaccggcagc gccgaagagc tgcggcagca aacgcaaagc gccacgctgg aagaagcatt
     3001 tattaatctg ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccgta
     3061 tcaacctgaa aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc
     3121 cttcgttgcc gttgatcacg tcaatttccg cattccacgc ggggagattt ttggattcct
     3181 tggttcgaac ggctgcggta aatccaccac catgaaaatg cttaccggac tgctgcccgc
     3241 cagcgaaggt gaggcgtggc tatttgggca accggttgat ccaaaagata tcgatacccg
     3301 ccgtcgtgtg ggctatatgt cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca
     3361 aaacctggag ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcgcgagt
     3421 ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt
     3481 gccgctcggc attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggagat
     3541 gttaatcctc gatgagccta cttctggtgt cgatccggtg gcgagggata tgttctggca
     3601 gttgatggtc gatctctcgc gccaggacaa agtgactatc ttcatctcca cccactttat
     3661 gaacgaagcg gaacgttgcg accgcatctc actgatgcac gccggaaaag tgcttgccag
     3721 cggtacaccg caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat
     3781 cgcctatttg caggaagcgg cagggcagag caacgaagcc gaagcgccgc ccgtggtaca
     3841 cgacaccacc cacgcgccgc gtcagggatt tagcctgcgc cgtctgttta gctacagccg
     3901 ccgcgaagcg ctggaactgc gacgcgatcc agtacgttcg acgctggcgc tgatgggaac
     3961 ggtgatcctg atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt
     4021 tgcggtgctc gaccgcgacc agaccgtcag tagccaggcg tggacactca acctctccgg
     4081 ttcccgttac tttatcgaac agccgccgct caccagttat gacgagcttg atcgtcggat
     4141 gcgtgcgggc gatatcacgg tggccattga gatcccgccc aatttcgggc gcgatatcgc
     4201 gcgtggtacg cctgtggaac tcggcgtctg gatcgacgga gcgatgccca gtcgtgccga
     4261 aacggtaaaa ggttacgtgc aggcaatgca ccagagttgg cttcaggatg tggcgagccg
     4321 acaatcgaca cccgccagcc aaagcgggct gatgaacatt gagacgcgtt atcgctataa
     4381 cccggacgta aaaagcctgc cagcgattgt tccggcggtg atcccgcttc tgctgatgat
     4441 gatcccgtca atgctaagcg cccttagcgt ggtgcgggaa aaagagctag ggtcgattat
     4501 caacctttac gtgaccccca ccacccgtag cgaatttttg cttggcaaac agctgccgta
     4561 catcgcgctg gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg
     4621 cgtaccgcat aaaggcagtt tcctgacgct caccttggcg gcgctgctgt atatcatcat
     4681 tgccactgga atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt
     4741 cggaacggcg attatcacat tgatcccggc gacacagttt tccgggatga tcgatccggt
     4801 agcttcgctg gaagggcttg gacgttggat cggcgaggtt tacccgacca gtcattttct
     4861 gactatcgcc cgcggaacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt
     4921 tatcccgttg ctgatagcca tcccgctggt gatgggctta agcatcctgc tgctgaaaaa
     4981 acaggaggga tgatgcgcca tttacgcaat atttttaatc tgggtatcaa agagttgcgc
     5041 agtctgctcg gtgataaagc gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg
     5101 tattcgtcag cgaccgttac gccaggatcg ttgaacctcg cgccgatcgc cattgccgat
     5161 atggatcaat cgcagttatc gaaccggatc gttaacagct tctatcgtcc gtggtttttg
     5221 ccaccggaga tgatcaccgc cgatgagatg gatgccggac tggacgccgg acgctatacc
     5281 ttcgcgataa atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccggat
     5341 attcaggtga acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc
     5401 cagaatatta tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa
     5461 ccgttggtat cgctggaaac ccggatgcgc tttaacccga accttgatcc cgcgtggttt
     5521 ggcggggtga tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg
     5581 gcgctgatcc gcgagcgtga acacggcacg gtggaacatt tactggtgat gccgataacg
     5641 ccgtttgaga tcatgatggc gaagatctgg tcgatggggc tggtggtgct ggtggtatcg
     5701 ggattatcgc tggtgctgat ggtgaaaggt gtactgggcg taccgattga aggctcgatc
     5761 ccgctgttta tgctgggcgt ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt
     5821 atggggacga tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg
     5881 ccgctgcaaa tgctttccgg tggatccacg ccgcgcgaaa gtatgccgca gatggtgcag
     5941 gacattatgc tgaccatgcc gacgacacac tttgttagcc tcgcgcaggc catcctctac
     6001 cggggtgccg gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc
     6061 gcgtttttca ccatcgcact gctgcgattc aggaagacga ttgggacaat ggcgtaagga
     6121 agggaaactt gacttcatac tgtgatgtca taaagtgaag tcataaggac atgccatgag
     6181 aaccaaggta caggctttac ggaagaaaca aaaaaataca ttggatcaaa tatttaaaac
     6241 tcctgttcct caagggatca agtggtctga tatagagtca ctggttaaag cattaggcgg
     6301 agaaattaag aaaggaagag gttcgcgttg caagttcata ctaaatatga gcgttgcgtg
     6361 tttccatcgg cctcatccgt cgccagatac cgataaaggc gctgtagaaa gcgtgtgtga
     6421 ctggttacta agtatagggg taaaaccatg atcaaactga agacgccaaa ctctatggag
     6481 attgccgggc aaccagctgt tattacctac gtgccagaac tgaatgcatt tcgtggcaaa
     6541 ttcctgggct tgtcaggtta ttgtgatttt gtctctgaca gtattcaggg gctgcaaaaa
     6601 gaaggagagc tttccctgcg agagtatctt gaggattgta aagctgcggg tattgagccg
     6661 tatgcgcgta cagaaaagat taagaccttt acgttacgct atccagaatc gctgagtgaa
     6721 cgccttaata atgcggctgc gcagcaacaa gtctctgtta atacctatat tattgaaaca
     6781 ctgaatgagc gtttgaatca tttataaatt ttagcgacgt acattacgca gaatatctgc
     6841 ctttacagga tctatctatg caactgaact taattttata aattaagttc agttaatatc
     6901 ttttctttcc ttgttttaga aataactcaa tcttccttcg gcaggcactg caaatgcccg
     6961 tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc acccatgtca
     7021 cctttcaaca cggcgatttc cagacagtcg tcgtggttga tgtgcacatg tagcgtggcg
     7081 acggagaggt cgtggtgatg atgctgggtg gaaacaatac ggctggctaa gtcgcgtttt
     7141 tcgtgttcat acacatacga cagcaccgcg aaaccttgtg tgccgtgctg ctgggtagct
     7201 tcctgcgcca gggcactacg cagaatatcg cggatagctt cggaacggtt gttataacca
     7261 cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt gatggtgact
     7321 cgttgcattg gcgttaaacc ttttctgagg tgcgacggcg cacggggaat gcgggtaata
     7381 ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct cccaccgcct
     7441 gggtttcggc gatttgtccg ttgtccatca ccatcactcg ctggcaaaag cgttccacca
     7501 ggcgtaagtc atgagtgatg aacaggcagg cagtgccaaa ctgttgttgt agctttttca
     7561 gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct tcatccagaa
     7621 tcagcaattt cggttcgacc gccagcgcgc gtgccaggca gacgagctgg agctggccgc
     7681 cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc gccttcagca
     7741 tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg cgcatcggtt
     7801 cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc actgatggag tcctgaaaca
     7861 ccatctgaat atcacggcgg aaggctttac gttgggcgcg attaagtttc gccagcgatt
     7921 cgccgtgcca gctaatattc ccttgtgagg gagactctaa gcctaccagc agccgcgcga
     7981 gggtgctttt tccgcagccg ctgcgtccca gcagggcgac ggtttcgccg cttttcaggg
     8041 tcagggaaac gttattcagc accgcctgat gttgatgttt tccgctaaat ccaccgtggg
     8101 catagtgatg ggaaaggccg cagacgttaa gtaaggtcat gatgccagct ccataccgta
     8161 gagggcgaga tgagcggaaa ccaggctgcg cgtcaccgta tgtttggggg cgttaaacag
     8221 cgtttctaca tcgccctgtt cgacaatttt accttgtgac atcaccgcca cgtcatccgc
     8281 cagacgcgcc acaacgccca tatcatgggt gaccagcagc attcccggcg cttgtttttg
     8341 cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga ggtcggtggt
     8401 cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa tcatcatgcg
     8461 ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg cggcgttttc
     8521 cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt tccctaacgc
     8581 caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg cgctgcgcgg
     8641 gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg taaggcgaaa ccggtttgcc
     8701 atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc ccagcgtcgc
     8761 ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca cgcgcccgcg
     8821 ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg cgatattacg
     8881 tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg atccagatga
     8941 tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag cgccagcccc
     9001 ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt aatcatcacg
     9061 ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc cgcgacgtgc
     9121 agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat caccgcgcct
     9181 gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga cgccagcaca
     9241 aactcgcgtt ggcgtagtga aatcaccaag ctgcgcacca tgcgtgcata ccacgcccag
     9301 tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac gccaaccata
     9361 aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg catggtggcc
     9421 tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc taatgtcagc
     9481 accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgc catcagccgc
     9541 gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc cggcgaaagc
     9601 aggcacgacg gcaaatcaat tgcctgtgga tcgtacggct gccaccactg gctggtgagc
     9661 gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca gcgggaagag
     9721 aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc agcgcggcgt
     9781 tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc agcgtaaagc
     9841 actgaatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc ccgacgccag
     9901 gccaggcaaa gatgttttcg ataatcatcg tcccgccaat caattcgcca atgtgcatcc
     9961 ccacggcggt gatcatcggc agcgaggcat tgcgcagaat atggcggcgt tcggtctgtt
    10021 tgtcattcag gccgcgtagc cgcgcccagg tgacgtgacg ctgaccggcg acgtccagca
    10081 tactggcgcg cagcagtcgc gcgttaatcg ccagcgacat aaaggcgatg gaaaccgcag
    10141 gtaaaatgat gtgctgccag ctgccgtagc ccatcgcggg tagccattgc agatacaccg
    10201 aaaacgccat cactagcaaa aatgccagcc agaagttggg catcgacacg ccaagaaacg
    10261 cgataaagcg cacggcgaaa tccggcaaac gatcgcggtg gcgcgccgcc cagataccga
    10321 gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc agcgtggcgg
    10381 gcaggaagtt cagcatatcg tccagtaccg ggcgttggct ggcgaatgag ataccaaagt
    10441 caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc tgatccagcc
    10501 cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta gataaacgca
    10561 gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg atcaccgagg
    10621 cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc aacattaagg
    10681 tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt aggggatatt
    10741 acccagctcc ggttttgata ccaccatcat cgagatgtag ctgataggca gataaaccgc
    10801 ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc tgacgttgcg tttcgtcatg
    10861 ggtcgccagc acttcgccga tctctttatc aatcagcggc ttgtcggcta atccttgctg
    10921 tgcctggaag tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt gtggatcgta
    10981 tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct gacgagcata
    11041 gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc gcatatcagc
    11101 ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg acagttcaat
    11161 gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg tccaaccggc
    11221 tttttccagc aactctttcg ctttctgcgg atcgtactgg cgcggtttca ggccgagatt
    11281 agcgtaaggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc catataacgc
    11341 gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta ctgccagctc
    11401 gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg gctgtgacag
    11461 ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc
    11521 ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc gggtagtcgg
    11581 gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc agtagttttc
    11641 gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc acggtccggt
    11701 gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg agggagcgat
    11761 aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg cgcttttcag
    11821 ggtaatttgc agctctgttt tattgagtgc tttaacatca acaatctggt ttgccagctc
    11881 cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg ccgccgcctc
    11941 ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga aggtccaggt
    12001 tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg aaccgtctgc
    12061 ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt taggcgtgta
    12121 aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtggtgattt catctggtgc
    12181 agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc ggcggagtgt
    12241 ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat gacgatttta
    12301 agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg gtgggcggat
    12361 cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg ggtgggggtg
    12421 cagatcgcaa ggcttaaatt ttctagctga caatggctga ctgacagcga ggagtgatag
    12481 gtgctatcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac gatggcttct
    12541 ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac ggcgtccatt
    12601 tcagcgtgtt ccccgaggct gaacacggcg ttcgccagcc agcgccagtt ggcgcgcggg
    12661 cgaatcactt cgatatcgca gccgacttcg ccttcatcgc tcaacagcag agcgatatcg
    12721 tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg gcgcgaatgc aggtttgcct
    12781 tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc
    12841 gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc cggtggcagt
    12901 ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc agggccaacg
    12961 tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg taaccgactt
    13021 ttcggtctgc ccggccccag taaaatcgcc agtttgctgc cacctttcgt cgtttccatc
    13081 cagattttac acacgctggt taatggcacc gaaagtagca tccctaccgg gccgagcagc
    13141 catccccaga ttaacaatga aagaaatacc accatggtgg acatccccag gcgatggccc
    13201 atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa caatgcgccg
    13261 accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg cggtacggcg
    13321 gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac tgcccacatc
    13381 agcgcaaact gtacgcccat tagcgccagc cccagccaga cgatgacacc tgtccataaa
    13441 ctgagtagcg tctttaacgc cagataatgc gaaacgcctt taagtgcgcg gtgtaatcct
    13501 gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac gtggcgcact
    13561 tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa
    13621 agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc
    13681 attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt
    13741 ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc
    13801 gccagtacgc cgaccagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg
    13861 gcaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg
    13921 gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg
    13981 aggatcacta ccagcgaggc caacttgagc agaatgtgca tgcccgtttt atcgggttga
    14041 ggggtttcca ttggggcttc cttgtgactt tttgtgttaa gtgtagcgcg ggagtcacgc
    14101 cagcaatatt ctgattctta ctgctgattt tcacgtcagc acatggtaaa aatgaaacac
    14161 tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc gctaaacgga
    14221 ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag ctacctcacc
    14281 atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg ctttagcgcc
    14341 ttctgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag ccgccctcat
    14401 gccggacgtt acgccgattt gctgggaccc aaaaagattg tcgtcttcgg tttatgcggc
    14461 tgctttttga gcggtctggg gtatctgacg gcaggattaa ccgccagtct gcctgtcatc
    14521 agcctgttat tactttgcct ggggcgcgtc atccttggga ttgggcaaag ttttgccgga
    14581 acgggatcga ccctatgggg cgttggagtg gttggctcgc tgcatatcgg gcgggtgatt
    14641 tcgtggaacg gcattgtcac ttacggggcg atggcgatgg gtgcgccgtt aggcgtcgtg
    14701 ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt ggcgctggtg
    14761 gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa accgctgccg
    14821 tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cgctggcact ggcttccgcc
    14881 ggatttggcg tcatcgccac ctttatcacg ctgttttatg acgctaaagg ttgggacggt
    14941 gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt gttattccct
    15001 aacggcatta accgtatcgg tggcttaaac gtagcgatga tttgctttag cgttgagata
    15061 atcggcctgc tactggttgg cgtggcgact atgccgtgga tggcgaaaat cggcgtctta
    15121 ctggcggggg ccgggttttc gctggtgttc ccggcattgg gtgtagtggc ggtaaaagcg
    15181 gttccgcagc aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat ggatttatcg
    15241 cttggcgtga ctggaccact ggctgggctg gtgatgagct gggcgggcgt accggtgatt
    15301 tatctggcgg cggcgggact ggtcgcaatc gcgttattac tgacgtggcg attaaaaaaa
    15361 cggcctccgg aacacgtccc tgaggccgcc tcatcatctt aaaacttact gaataaccag
    15421 cgtattaatg atgttttctg cggtggtttg cgctttttgc tgatcgtcag ctggcagcgt
    15481 aatttgcatg gtcagcagtt gattacccac gttacccaga ataacggaag agtacgccgt
    15541 ctggcctttc gcggagataa tactgtctaa ctgctgcatt ttgtgacctt tcagctcaat
    15601 ggctttattg gttaccactt gcagctgcgg atcgcggcta cgttgctgat cttccagacg
    15661 cttcgccaac accgccagat cttctttcgg atcatcgccc atgatgacga tgactgcttt
    15721 ctgcccggtg gcgtcggacc agacatgcat gttattggcc tgcgttccca gcttaccgct
    15781 ctggtcggtc atatccgctg gcagcgagaa acttaacttg ccatcaagca ggttgacggg
    15841 attcccggta gcgttacttt ccgcaaccga accctgcgcc gtagcgttag tgtctttatc
    15901 atcacaggcc gcaagcccca taaccagcag gccaattccg acatatttaa ccagattgcg
    15961 cattgacttc ttcctttcga taaacggcca taacggctca ttcatccatc ttatcacaac
    16021 tctgataacg aacctttaac tcgcctgcaa agcgttgatt tcggatttat ctgccagtct
    16081 tttcaacagc atattgagta atacgccata cattggcagg aagaaaacga tactgattaa
    16141 cactttgaaa cagtaatcga ccagcgcgat ttccatccag tgttcagcca taaaggcatc
    16201 cgggctacgc cagaaggcaa tgaagaaaaa ggccagcgtg tcgctgacgt taccgaacag
    16261 tgtggacgct gtcggtgcca gccaccagcg gcgactctga cgcaggcggt taaaaacgtg
    16321 cacgtcgagg atttgcccca gcgcgtaggc catgaaactg gcggtggcga tacgggcgac
    16381 aaacaggttg aagtgggcga gtgcgccgaa tccctgccag gaacccatat agaatagcga
    16441 cgagatgacg taggagatta ataacgcagg gatcattacc gcgaagataa tgcgtcgggc
    16501 cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca agaaaaataa acggaaagct
    16561 aaacgcgccc caggtggtat ggaaaccaaa aatggagacg ggaagctgaa ccagatagtt
    16621 actggaggtg atcaccagaa gatgaaataa cgataaccag aacaacgcct tatagcgttg
    16681 agtttgcgag aaaacgttca tattgtacct ttttgattaa ccattggggt gagggaaccc
    16741 aatacgtacg acacgtctgt taccttaaca taataacgtg cttacccttt tttcgagccg
    16801 ccgcatgata ctgctttacg ttgacaatgc aatggttaat tttcacgcaa tcgttaacct
    16861 ggtttgctta cggactcaca gggcgtaaac tagcgccgtt tttttaagtg atgagaagaa
    16921 aatgaccgat ctcttttcca gccctgacca cacactcgac gcgcttggcc tgcgctgccc
    16981 ggaaccggtg atgatggtgc gcaaaaccgt gcgcaatatg cagcctggcg aaacgttgct
    17041 gattatcgcc gacgatccgg ccactacccg cgatattcct gggttttgta cctttatgga
    17101 acacgaactg gttgcgaaag agacggatgg actgccttat cgttatttga ttcgtaaagg
    17161 cggttgatag gggctgattg gcttcgatgc cgccttttcc tcacccccta accctctccc
    17221 cagaggggcg aggggaccga tcgcgctcaa tgttgcgatc ggtttgcctt atctcctgcg
    17281 caacaacctt aacgcattcg ccgtcaccag caccgtcgca cccgtatctg ccagcactgc
    17341 cagccacaac ccggtcatcc ctaaaagcgt ggtgacgagg aagatccctt tcagtcccag
    17401 cgcaatggtg atgttctggc ggatattggc gtgagtggcg cgtgccagtt caatcatttg
    17461 caccaggccg cgcaggtggt tatgggttaa tgctgcgtcg gcggtttcca gcgccacgtc
    17521 tgtgccgcta cccatagcaa tcccgatggc ggcagctttc atcgctggcg cgtcgttaat
    17581 accatcaccg accatcgcca ggggcgcatg ttgattcagc ttggtcaccg ctttgacttt
    17641 atcttccggc aataggcccg ctttaaactc cagccccagc tccccggcaa ttgccgccgc
    17701 tgcgcgtgga ttatcgccgg tgaggatcac tcctttaacc cccagcgcgt tcagttcact
    17761 gatggcagtt gtagcgtcgg cgcgcagggt atcctgcaat gcaatgatac ccagcacgtc
    17821 atcattacgt actaccagca caaccgtttg cccggcgctt tccagttcat taatcaaacc
    17881 agcaaatgca tcagcgggat gtttcccggc agcgcatatc aacacgcgct caccattaac
    17941 ctgcgcttca atgccagacc cgaccagtgc ccgctgtgat tcggcggtgg gaatggcgag
    18001 ttcagcaacc tgtgcttcgc gtacgatggc ttgcgccagt ggatgcgtcg cgccttgctc
    18061 gaccgccgcc gccagtgtca gcagttcaga ttcactaata cccgttgccg gatgaatcgc
    18121 ggtaacgcgc ggtttaccga cggtcagcgt accggtttta tcaaacgcca cttgggtaac
    18181 acgacctagt tgttccagcg ccgcaccgcc tttaattaac gccccacgac gcgctgctgc
    18241 tgccagcccg gaggttatcg ccgcaggcgt tgagataact aacgcacacg ggcaaccaat
    18301 cagcagcagc gtcagacctt tataaatcca ctcctgccag ctggcggcaa acagcagcgg
    18361 cggcactagc gttaccagca gggcgacggc cataatcgct ggcgtataaa tacggctgaa
    18421 acggtcgata aaccgctcaa tgggggcgcg gcgctcttcg gcttcttcga tcagtttcag
    18481 aatccggtca atggcgctgg cacccggttc tgacagcact tccagcgtta ccagacggtc
    18541 tacgctggtg gctcctgcag gaactttatc gcccgtcgcg cgctccaccg gaatggattc
    18601 tccggttagg gcgctttcat caaaactggc aaacggtgag agcagtttac cgtcggcagg
    18661 caaacgccca cctgcggcga cttcaatcac atcgccaggc cgcaggctgt taatcgccac
    18721 ctcttcccgc tcaccgttac gcaggcgcgt ggcggtttct ggtttcagcg ccattaacgc
    18781 gctaacgccc tgacgcgcgc ggctggcggc ccagccttcc agtcgttcac caatcaaaaa
    18841 cagcagcaac accatcgcag cttcagccgt tgcgccaata aacagtgcac caatagcggc
    18901 tacgctcatt aaggtttcaa tggcgaagta gctgccggat ttgatcagcc gtaatgcctg
    18961 acgagcaatc gggtacagcc caactagcgt ggtcgcgata aacgccagtt gcccgaacgg
    19021 atgattgaac tgctccagac cccagctgat tgccatcatc acgataagcg taatcagcgg
    19081 cagattctct ttcaggcgcg atgcttgtgg ctcgtcggcg gcctgctcat cgcgcaggga
    19141 atagcccgct ttttgcaccg cagattcaac ctgggcacgg atgtcattgt cggcatcaac
    19201 caccagtttt tcggtggcga acaacacctg cacctgattc acgcctgcaa gctggcgcac
    19261 ggcattttct accttgcgcg cacaggcggc gcagtccatg ccgctgactt tccagctata
    19321 gcgggtgccg gagacgtttt cagagagcgt tggcgagctg gagcatgcgc cgtcgcagca
    19381 acagtcgttg gcgttctgta ccgtggttag cggtttgaac gcagcaaatt gaggggcttt
    19441 cttgccgtga ttgtcaggtg tcgacatggc atcctccggt taagtttttt ctcattaacc
    19501 gaaggataca ctctggagtc gactccagag tcaagtttta tcagagatac agtgagcgga
    19561 cgatgaggaa gtgaccggca aagtagcagg ccgcagcaat cgcgttatcc gcacggaagc
    19621 gtcggcgata gtggctcccc agccagacaa agttactgat aaacagcaac gaagcgccaa
    19681 caaacgcaga gagcgccgga gcggtcggac ggaagaacca cagttcacct gccagccaca
    19741 ccatcaccag cgtcatgccg ataaacgtgc agataggcca acggtactct tccagccgcg
    19801 tccagataat cgccagtagc agcgcaccca gaaccagcaa caccagtggc agaggccaga
    19861 agaaagagag cgtcatctga ctggcgaaat agatggtgta cagcaggtgc gagaggaaaa
    19921 acgcgccgat ggcgtacatc agacgttgac gtggcaacag ggttagcgca tcgcccagca
    19981 gtgaggcgca cagtcccgcc agcaccagat agctaatggc gtcgaacatc ggtgcttgcc
    20041 aggccagtaa cagcaggaga agaagggtta acggtttaaa cacccagcgt tgccaggttg
    20101 gcccacgata cgatgcatcc acagatagcc atgcggaaag acagacagcg ataaacgacc
    20161 aaagcatctt agttccttga attcgttatt ttttcgtaag cagaatgcct acggtcttgc
    20221 ttccagttta gtggcgaggg tggacggatg acaacaccat aactggcagg gtatgcttaa
    20281 ttccgcattt tccgatgagg gataaagatg agtaaaccac ctcttttctt tattgttatc
    20341 attggcttaa ttgtcgtcgc cgcatcgttt cgttttatgc aacagcgacg ggaaaaagct
    20401 gataatgata tggctccgct ccagcaaaag ctggtggtgg tgagcaacaa gcgggaaaaa
    20461 ccgattaacg atcgccgttc gcgccagcag gaagtgactc cgg
//
LOCUS       DAJTUN010000070        20498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTUN010000070 DAJTUN010000000
VERSION     DAJTUN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.070"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..793
                     /locus_tag="OO470_002385"
     CDS             <1..793
                     /locus_tag="OO470_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6251608.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="OO470_002386"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="OO470_002386"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6251609.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OO470_002387"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OO470_002387"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6251610.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OO470_002388"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OO470_002388"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6251611.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="OO470_002389"
     CDS             complement(4991..7225)
                     /locus_tag="OO470_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6251612.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OO470_002390"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OO470_002390"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6251613.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="OO470_002391"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="OO470_002391"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6251614.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OO470_002392"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OO470_002392"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6251615.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="OO470_002393"
     CDS             complement(12980..14320)
                     /locus_tag="OO470_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6251616.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OO470_002394"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OO470_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6251617.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFYNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="OO470_002395"
     CDS             complement(16108..16557)
                     /locus_tag="OO470_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6251618.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OO470_002396"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OO470_002396"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6251619.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="OO470_002397"
     CDS             complement(17357..17686)
                     /locus_tag="OO470_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6251620.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OO470_002398"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OO470_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6251621.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="OO470_002399"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="OO470_002399"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6251622.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..>20498
                     /gene="ppnN"
                     /locus_tag="OO470_002400"
                     /gene_synonym="ygdH"
     CDS             19881..>20498
                     /gene="ppnN"
                     /locus_tag="OO470_002400"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6251623.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRY"
BASE COUNT         5042 a         5475 c         4880 g         5101 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc atgccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgtaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatat
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttac
//
LOCUS       DAJTUN010000071        20433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTUN010000071 DAJTUN010000000
VERSION     DAJTUN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.071"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..306
                     /locus_tag="OO470_002401"
     CDS             <1..306
                     /locus_tag="OO470_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6251624.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2206
                     /locus_tag="OO470_002402"
     CDS             278..2206
                     /locus_tag="OO470_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6251625.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2190..2393
                     /locus_tag="OO470_002403"
     CDS             2190..2393
                     /locus_tag="OO470_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6251626.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2390..3983
                     /locus_tag="OO470_002404"
                     /pseudo
     CDS             2390..3983
                     /locus_tag="OO470_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3973..5478
                     /locus_tag="OO470_002405"
     CDS             3973..5478
                     /locus_tag="OO470_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6251627.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5515..5862
                     /locus_tag="OO470_002406"
     CDS             5515..5862
                     /locus_tag="OO470_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6251628.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5920..6948
                     /locus_tag="OO470_002407"
     CDS             5920..6948
                     /locus_tag="OO470_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6251629.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7000..7386
                     /locus_tag="OO470_002408"
     CDS             7000..7386
                     /locus_tag="OO470_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6251630.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7398..7775
                     /locus_tag="OO470_002409"
     CDS             7398..7775
                     /locus_tag="OO470_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6251631.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7762..8346
                     /locus_tag="OO470_002410"
     CDS             7762..8346
                     /locus_tag="OO470_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6251632.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8343..8744
                     /locus_tag="OO470_002411"
     CDS             8343..8744
                     /locus_tag="OO470_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6251633.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8755..9495
                     /locus_tag="OO470_002412"
     CDS             8755..9495
                     /locus_tag="OO470_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6251634.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9554..9940
                     /locus_tag="OO470_002413"
     CDS             9554..9940
                     /locus_tag="OO470_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6251635.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9949..10278
                     /locus_tag="OO470_002414"
     CDS             9949..10278
                     /locus_tag="OO470_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6251636.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10250..12988
                     /locus_tag="OO470_002415"
     CDS             10250..12988
                     /locus_tag="OO470_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6251637.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12988..13317
                     /locus_tag="OO470_002416"
     CDS             12988..13317
                     /locus_tag="OO470_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6251638.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13317..14015
                     /locus_tag="OO470_002417"
     CDS             13317..14015
                     /locus_tag="OO470_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6251639.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14020..14763
                     /locus_tag="OO470_002418"
     CDS             14020..14763
                     /locus_tag="OO470_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6251640.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14700..15302
                     /locus_tag="OO470_002419"
     CDS             14700..15302
                     /locus_tag="OO470_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6251641.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15363..18843
                     /locus_tag="OO470_002420"
                     /pseudo
     CDS             15363..18843
                     /locus_tag="OO470_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            18911..19510
                     /locus_tag="OO470_002421"
     CDS             18911..19510
                     /locus_tag="OO470_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6251642.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19574..>20433
                     /locus_tag="OO470_002422"
     CDS             19574..>20433
                     /locus_tag="OO470_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6251643.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPG"
BASE COUNT         4782 a         4830 c         6445 g         4376 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc cgtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgcgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca cctgggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtgttttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgctggcgg catcccgcaa ggatgcaact gcacaaccga cccttgaaga
     2161 actggcagcg aagctgtccg gaggagtgaa tggttacagt cgctgaactg caggcgctgc
     2221 gtcaggcgcg ccttgattta ttaaccggta aacgggttgt gtcggtacag aaagacggtc
     2281 gcaggatcga atatacggca gcgtccctga atgagcttaa ccgtgcgatc aacgatgcgg
     2341 agtcggttct gggtacgacc cgacgccgcc gaccgctggg agtgaggtta tgaaacgaac
     2401 gcctgtcctg attgatgtga acggcgttcc gcttcgtgag agtctcagct acaacggagg
     2461 cggcgcagga tttggcgggc aaatggcaga gtggttacca ccggcgcaga gtgccgatgc
     2521 ggccctgttg cctgcattgc gtctggggaa tgcccgggca gatgatctgg tgcgcaataa
     2581 cggtatcgcg gctaatgcgg tggccctgca taaggatcat atcgtcgggc acatgtttct
     2641 tatcagctac cgtctgaact ggcgttggct ggggatgcgg gagaccacgg caaaaagttt
     2701 tgtcggggag gtggaagcag cctggtcgga atacgcagaa ggaatgtttg gcgagatcga
     2761 cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa ggggtgggcg tgcatgcctt
     2821 taacggcgaa atctttgtgc agccggtctg ggatacggaa accacgcagt tattccgtac
     2881 gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca ggacacggta tggggaacca
     2941 ctttctgcgg gccggggttg aggtcgatcg gtatgggcgg gcgattgcgt accatatctg
     3001 tgaggatgat tttccgttct ctggtgccgg acgctgggag cggatcccgc gtgagcttcc
     3061 caccgggcgt ccggccatgc tgcatatttt tgagccggtg gaggacgggc agacccgtgg
     3121 ggccaatcag ttttacagcg taatggagcg gatgaagatg ctggactccc tgcaggccac
     3181 gcagcttcag tcggccattg tgaaggcgat gtatgcagcc acaatcgaaa gtgaacttga
     3241 taccgaaaag gcatttgaat atatcgcggg ggcaccgcaa ggtcagcagg ataatccgct
     3301 tattaatatt ctggagaagt tcacccgctg gtatgacacg aataacgtga cgctgggcgg
     3361 tgttaaaatt ccgcaccttt tttcctggcg atgcgctgaa cctgcagaca gcgcaggatt
     3421 cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatattgca gccggtcttg
     3481 gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttactca agtgcccgtg
     3541 cctccgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt gcggcccggc
     3601 tggccacgca gatgttttcc tgctggctgg aagaggcgct tcttcggggg attattcgtc
     3661 cgccacgggc gcgttttgat ttttaccagg cgcgttcagc ctggtcacgg gcagagtgga
     3721 ttggctccgg aagaatggcc attgacgggc tcaaggaggt ccaggaatcg gtgatgcgca
     3781 ttgaggccgg actgagcacg tatgagaaag aactggcact gatgggcgaa gattatcagg
     3841 acattttccg ccagcaggtc agggaatccg cagagcgcga aaaagccgga ctctcacgtc
     3901 cggtgtggat agcccaggcg tatcagcagc agatagcgga gagtcgcagg ccggaagagg
     3961 agacaacatc acgtgagacg taatctttca catattatcg ctgcggcatt caatgagccg
     4021 ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg tgagatgggc
     4081 gcagcaagcc tttcggtacc gcaacagcag gtacagcttg acgctcccgg gatgctggct
     4141 gaaacggacg agtacatggc cggaggcaaa cgacctgccc gtgtttaccg ggtggtgaac
     4201 ggtattgctg tactgccggt gaccggcacg ctggtacacc ggctgggtgg catgcggcca
     4261 ttttccggaa tgactggcta tgacggcatt gtcgcctgtc ttcagcaggc aatggcggac
     4321 actcaggtac ggggcgtact gctggacatt gacagtcctg gcgggcaggc caccggcgcg
     4381 tttgactgcg ctgacatgat ttaccgtctc cggcagctga agcctgtctg ggcactgtgc
     4441 aatgacacgg cctgttcagc cgccatgtta ctggcatcgg cctgctcccg acggctggtt
     4501 acccagacat cccgtatcgg ctccattggg gtgatgatgg gccatgtcag ctatgccggt
     4561 catctggcac aggccggtgt ggatatcacg ctgatttacg ccggatcgca caaggtggat
     4621 ggcaatcagt ttgaatccct gccagcaaag gttcgccagg acatgcagca gcgcattgat
     4681 gcggcgcgcc ggatgtttgc tgaaaaagtg gcgatgtata ccggtttatc tgtggatgcg
     4741 gttatgggaa cagaggccgc cgtttttgaa ggtcagtccg gtattgaggc cgaactggcg
     4801 gatgaattaa tcaatgcgtc ggacgccatc agcgtgatgg ctgcgacgct gaacacacac
     4861 gatacaggag gcactatgcc gcaattaact gcaacggagg ctgccgcgca ggagaaccag
     4921 cgagtgatgg ggatcctgac gtgtcaggaa gcgaaaggac gtgaacaact ggccacgatg
     4981 ctggcagggc aacagggtat gagcgttgag caggcccggg cgattctggc cgtggcggca
     5041 ccgcaacaat ctggtgtatc tgcgcagagt gaagccgatc gcattatggc atgtgaagag
     5101 gcgaaaggac gtgaacaact ggcggcaacg ctggcggcga tgccggagat gacggtggaa
     5161 aaagcccgtc cgattctggc tgcttcaccg caggcgaatg ccggaccctc acttcgtgat
     5221 cagattatgg ccctggatga ggcaaaaggg gcagaggcgc aggctgaaaa actggcggcc
     5281 tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg cggcagcctc aggcagggca
     5341 gaaccggttt ctgcatccac aaccgccatg tttgaacatt tcatggcgaa ccattcaccg
     5401 gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga cgtgaaaatg
     5461 ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttta caatatggta
     5521 acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa tgatccggcc
     5581 cataccgcca caggcagcag cgggatttcc tcggcaacac cggctctgac gcccctgatg
     5641 ctggatgagg ataccgggaa actggtggtc tgggacggac agaaggcagg ccatgtggtt
     5701 ggcatactgg tactgccgct tgaaggtaca gagacggtgc tgacgtatta caagtcgggg
     5761 acctttgcga cggaggccat ccactggcct gagagagtgg atacacacaa aaaggcgaat
     5821 gcctttgccg gcagtgccct gagtcacgca gcgctgccgt aacattttat caggtcactg
     5881 cggtagcctg actgatttct gaatgaaagg aactgattta tgggattgtt tacgacccgc
     5941 cagttactcg gttataccga acaaaaagtt aaatttcgtg cgctgtttct ggagctgttt
     6001 ttccgccgta cggtgaattt ccataccgaa gaggtaatgc tggacaaaat taccggaaaa
     6061 acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aggtgttgcg tcaccgtggc
     6121 ggtgaaactc gtgtactgcg cccgggctac gttaagccga aacacgaatt taattaccag
     6181 caggcggtgg agcgtcttcc cggtgaagat ccggcccagc tgaacgaccc ggcttaccgt
     6241 cgcctgcgta tcatcaccga taacctcaaa caggaagagc acgccattgt ccaggtggaa
     6301 gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg agagcagttt
     6361 gaaactgttg aggtcgattt tggccggtcg gtgggaaata acattacgca ggcaggaggg
     6421 acggagtgga gcgctcagga ccgcgatacc tttgatccga cgcatgatct ggatgcgtac
     6481 tgtgattttg cttccggcac catcaatatt gccattatgg atggtaaggt ctggcgtctg
     6541 ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc gtcgtggctc aaattcacag
     6601 ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct tcaaagggta ttatggtgat
     6661 ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg acggtaccga aaaacgttat
     6721 ctgccggacg gcacactggt actgggaaat acggccgcag agggtattcg ttgctacggt
     6781 gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg cgtcttcccg ttatccgaaa
     6841 cactggctga ccgtgggtga tccggcgcgt gaattcacca tgacgcagtc cgcgccgctg
     6901 atggtgctgc cggatccgga tgagtttgtg gtggtgcagg tgaaataatc cgtgagcggg
     6961 agctaaatgc tcccgttttt cattcaatca ggagcgggta tggctacgaa agaagaaaac
     7021 ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg aaccggatgt ctccggtagt
     7081 gcggcagata ttgcgcagcg agtggcggaa ctggaggaag agcttggaga tgcgggggag
     7141 gtcgcagagc cggatacgta ctcaccatcg aaagatgatc cgaccgcgca taaaggcgag
     7201 tcagttcctg aacagccagg cattgttacc ggagacgaaa cggggcaggt tacggtggtg
     7261 gcactggcga cactgcatac aaaatgtaat ggttcagtga tatttgtttc accgggaacg
     7321 tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg caggtggtct ggcaaaaagg
     7381 cagtaacagg agatgtcgtg gacggtttcg ataatctgtt tgatgctgcg cttgccggtg
     7441 tcaatgaagt cattctccgt gatatgggga tcagcgccgt cattacgtcc ggagaacttg
     7501 aggggacaca cctgacaggg gtttttgatg atcccgaaag tatttctttt gttgccgggg
     7561 ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa aacggcagat atatcgcagt
     7621 tacgccgtca ggatacgtta actatcgggg atgattcttt ctttgtggat cgtattactc
     7681 cggatgacgg gggatgctgt tatatccggt taaggcgggg cagtcctgcc ccacagaata
     7741 atgccagaat gaggcgatat gatgaaggga cttgagaatg ccatccgcaa tctgaacagc
     7801 ctcgataccc gcatggtgcc acagaccagc gcatgggcgg taaaccgtgt ggcggcaaag
     7861 attgtttctg tcgccacacg gcaggttgcg cagaataccg ttgccgggga taaccaggtg
     7921 aaaggtattc ccctgaaaac ggtcagggag cgtgtcaggc tgcttaaagc cagcccctca
     7981 ggaaaaatgt atgcccgcat gcgtgttaac cggggtaacc tgcccgccat taagctgggt
     8041 accgcacagg tcagactggc ccgctcccgg catggcagca actcacgtca tcgtggcagc
     8101 gtactgaaag tggggaaata ccttttccgg gatgcgttta ttcagcaact ggcgaatggt
     8161 cgctggcatg tgatgcggcg tattgacggc aaaaatcgtt accccattga tgtggtgaaa
     8221 atcccgatgt ccggaccgct gacacaggca tttgaagatg cccgcgaccg catcattgct
     8281 gcggaaatgc cgaaacagct ggggtatgcc ctgaaacaac aactgaggtt acatctgagt
     8341 aaatgaaccg acacacacaa atccgtcaga gcgtgctggc acgccttcgg gaacagtgtg
     8401 gagacagcgc cacgtttttt gacgggcttc cggcatttat tgatgcgcag gaactgcctg
     8461 ccgtggcggt gtggctgagt gatgctcagt acaccggaaa aatgacggat gaagatgact
     8521 ggcaggctgt tctgcatatt gccgtcttca tccgggcaca ggcaccggat tcagagctgg
     8581 atatgtggat ggagagcacc attttcccgg ccctgaatga tgtaccggca ctttccggac
     8641 tcatcgacac cctgaaccca ctcggtttta actatcaacg tgataatgag atggctacct
     8701 gggcgatggc ggaaatcacg taccagatca cgtacacgaa ttaaggaggt ggtaatgacc
     8761 acaccaaatc cactggcaaa gacgaaaggt gcggggacga cgttctggat gtataccggc
     8821 aacggcgatg cgtttgcgaa ccctttgtcg gacactgact ggctgcgtct tgcgatggtg
     8881 aaggatctgc aacctggcga aatgaccgct gatgcagaag atgacactta tctcgatgat
     8941 gaagatgcag actggaaaac gacaacccag gggcagaaat ccgttggtga tacttcggcg
     9001 acgctggcct ggcgtccggg tgacagcggg cagaaaaaac tggttcagtt gttcgactcc
     9061 ggtgaagtct gcgcgtttcg tatcaaatat cccaacggca ctgttgatgt tttccgcggc
     9121 tggctgagct cactgggtaa aaccattgcc tcaaaagacg tgatgacccg cactgtgaaa
     9181 atcagcggtg tggggcgtcc gtatctggca gaggaaggca ctgaaaccgt gggtgttacc
     9241 ggactgacgg tggcaccggc atctgtcagt gtcaaagcgg gagcaaccac cacactgacc
     9301 tttacagtaa aacctgacgg ggccagtgac aaagcgatca gtgtgcattc gtcagatcct
     9361 cagactgctt cggtgaccct gagcgggctt gtggccacgg tgaaaggcgt gaagcagggc
     9421 agtgtcagca tcgtgggcat gacctctgac ggagagtttg tggcagtggc tgcggtgacc
     9481 gtcagcgcac cataacagga cgatactcat cattgccccg gttatccggg gctttttgca
     9541 tccggagaac atgatgtttc tgaaacagga tacgtttaat tatgaaaaac agtccgtggt
     9601 gctcagtgag ctgtccgggc tgcagagaat tgaatatctg acgtttgttc agcagcgaac
     9661 ggcaaagttt gatgcacagg agggagaact gccggaggct gaacgacaga ttgcttttct
     9721 gcgtatggga atggatatca atgcctggct ggtttcccgc tcactgtgga atgctgagca
     9781 gtctcaggat gttgagacgc tttgcgcatc cattatgaca acatggtcgt atgatgcgct
     9841 gggcgcgggg gcggagaggg ttctgtcgct gagcggtatg gggaccattg agaatgccgg
     9901 ggatgatgat catgaggcgc tgacgccgga aaagtcctga cgcgggaaat gcagtttgtc
     9961 atgcggcttg cccgggagtt ccggcgggca gactggcggc ggatgctgtc ggatatgtcg
    10021 gccactgagc ttggtgagtg gggcgattat ttccggatgc agagcttcag tgatgtgtgg
    10081 atggatgcgc agtttgcctc gctgaaggca ttgatcgtga gaatggtgtc cggcagcagc
    10141 gatgctgcgg tggctgattt cagcctttta ccggaagaga acgggatacc ggagcgaacg
    10201 gacgaagaac tgatgcatct tggggaaggt atttccggag gtgtgcgtta tggaccagat
    10261 agccaacctg gtcattgatt tggggattga tgcggcagag tttaaaaatg aaattccccg
    10321 tatcaaaaac cttctgaatg gtgcagccag cgatgcagaa cggtcttctg cccgtatgca
    10381 gcgttttatg gagcgtcaga ctcaggcggc ccggcagaca atgcaggcgg cttcttcggc
    10441 tgcaacagca gcatcagccc atgcgcagac ggtggagaag aatgcacggg ctcatgaacg
    10501 catggcccgt gaggtggaac aaacccgtct gcgcgtggat gccctgaatc agaaaatgcg
    10561 cgaggaacag gcgcaggcca gggcggctca gctgggggta agcagtgctg cagaagtgta
    10621 tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga aaagtgcggc
    10681 agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt ttggtgcgct
    10741 gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac tgatgtcgcc
    10801 gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc tgggtctggg
    10861 taaagcctgg catgacggcc ggaaggaggg cgagaaattt aaccgtcagc tggcgctgac
    10921 gggacattat gccggtgtca ctgtcgggca gttgtggaaa ctcagccggg ccatatccgg
    10981 gaatggtatc acgcaacatg cggcagccgg tgcgctggca caggtggtgg gcagtggggc
    11041 atttcatgga aacgatattg gtatggtggc gaaagctgcc gcacagatgg agcgatcggt
    11101 tggccagtcg gtcagcgata ccataaatca gtttaagcgg ctgaaggatg atcctgtaaa
    11161 tgccgcgaag gcgctggaca atgcgctgca ttttttgaat gccactcagc ttgagcagat
    11221 acgcgtcctt ggggagcagg ggcggtccag tgatgcggcc cggatcgcca tgtctgcgct
    11281 ggcagaggaa accggtaaac gcacatctga tattgataat aatctcaatg cgctgggtag
    11341 tacgctgcaa accttgtctg actggtggaa gcagttctgg gatgcggcca tgaatattgg
    11401 tcgtgaagac tcgctggatg cgcagattga tgcgttacag gaaaaaattc agcgcgcgaa
    11461 aaaatatccg tggacaaacg cctccacaca ggtggagtac gatcagcagc gtcttaacga
    11521 tcttcaggag aaaaaacgcc ggaaggattt gcaggatgca aaagcgcagg cagaacggaa
    11581 ttaccaggag caacagaaac gccggaatgc tgaaaatgcc gcgctgaacc ggatgaatga
    11641 aacggaagct gcacgacatc agcgggaaat tgcgcgtatt aatgccatgc agtacgccga
    11701 ccaggctgtc agggatgcgg cgatacagcg tgaaaatgaa cgttacgaaa aagccattaa
    11761 gaaaaataca cgggcaaccc gtaatgatga ggccacccgg ttattgctgc agtacagtca
    11821 gcagcaggca caggtggaag ggcagattgc tgctgccaga cagtcagcag gcattgccac
    11881 tgaaaggatg actgaagcgc ataaacagct tctggccctg cagcagcgca tcagcgacct
    11941 ggacgggaaa aagctgacgg cagatgaaaa gagtgtgctg gcccgtaaga atgagctgat
    12001 tcaggcgctg acgctgctgg atgtgaaaca gcaggagctg cagaaacaga cagcgcttaa
    12061 cgacctgaga aaaaaaacgg ttcagctgac cagccagctg gcagacaaag aacgtgcact
    12121 gcgtgagcag cacaatctgg atattgccac tgcaggtatg ggggataagc agcggcagcg
    12181 ctaccaggca cagttgcgca tccggcagga ataccggcaa cagttgcaac agcttgagaa
    12241 tgacagtcgc cagaaaggca cttacgggac ggaggactac cggagggctg aggaggtgct
    12301 gaaggggagc ctgaagcgac aactgaatga aaacaaacgc tactggcagg aactggaagt
    12361 ggcgcagggc gactggaaaa acggtgccat gcgggcgttt cagaatttta cggcggatgc
    12421 ggataatgcg gcgggaacgg cagaacagat gtttacagtg gcattcagca gtgccggtaa
    12481 tgcactggcg acattctgta ccaccggtaa gctgaatttt aaatccttca cctcttccct
    12541 gttgtcagat atggccagaa ttatggcaca gatggccatg atgcaggcgg taaagggcgt
    12601 cggttcttta ttcggcttca cgactaatgc tgatggcggt gtttaccagt ctgctgattt
    12661 gagtcgctac agtggcacgg tggttaaccg tccgacgttt tttgcttttg caaaaggcgc
    12721 gggtgtgatg ggggaagcgg gacctgaagc cattctgcca ctgcgtcgcg gtgctgatgg
    12781 taagctgggg gttgtggcgg atactggtgg ttcaggcatg gtgatgtttg ccccgcagta
    12841 caacattgag atcaataacg acggcacgaa cgggcagata ggtccggctg ccctgaaggt
    12901 ggtttatgac ctcgggaaaa aagcggccgc ggactttatg caacagcagg cccgtgatgg
    12961 tggccggtta agtggagcat accggtaatg gagacgtttc actggaaagt gcgcccggat
    13021 atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc tgggcgatgg ttatgaacag
    13081 cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca gcgtgacgat acgtgttcgt
    13141 aaaggtgaac acccatcttt aaaagccttt ctggaacggc acggtggcgt ccgcgcattt
    13201 cagtggacgc caccttatga ctggaaactg atcagggtgg tttgtcgtaa atggtcggca
    13261 agcgtggggg cgttatgggt gactgtaacg gccgattttg aacaggtggt gaactgatgc
    13321 aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc ggagcagtca gcccgggtgg
    13381 agctctggga aatcgatctg acagaggtcg gtggtgagcg ttattttttc tgtaatgagc
    13441 agaacgaaaa aggtgagccg gtcacctggc aggggcggca gtatcaggca taccccattc
    13501 aggggtcggg gtttgaactg aacggcaggg gctgtgctgc ccgtccgaca ctgacggtct
    13561 ctaacctgca cggcatggtc accgggatgg cggaagatct gcagagtctg gtcggcggaa
    13621 cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc gtcaacggaa
    13681 acagcgacgc cgatccggaa caggaggtga tcagccgctg gcgcatcgag cagtgcagcg
    13741 aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc aacggagacg gatggcgcgg
    13801 ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg gacttaccgt tctgatgagt
    13861 gtggttacac gggcagggca gtggctgacg agttcgacaa accaacaacg gatatccgga
    13921 aggacaaatg cagcaagtgt atgcgcgggt gtgagttgcg caacaatacc ggtaatttcg
    13981 gcggtttcct ttccatcaat aaactttctc agtaaatcca tgacacagac agaatcagcg
    14041 attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gtggcttcgt ggtgagaacg
    14101 ccggagggag acaggtatct tcccagcgag aatatctccg gtgagccgga ggaacggttc
    14161 cggatggctc cggaggactg gctgcgggca caaatgcagg gtgagattgt ggcactggtc
    14221 cacagtcatc ccggtggtct gccctggctg agtgaggctg accggcggct gcaggtgcag
    14281 agtgatttgc cgtggtggct ggtctgccgg ggggcgattc acaagttccg ctgtgtgccg
    14341 catctcaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat
    14401 gcttatcatc tggcggggat tgagatgccg gattttcatc gcggggatga ctggtggcgt
    14461 cacggtcaga atctctatct ggataatctg gaggccacag ggctgtatca ggtgccgttg
    14521 tcatcagcac aaccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat
    14581 gccgccattt actgcggcga tggtgagctg ctgcaccata ttcctgaaca actgagtaaa
    14641 cgagagaggt ataccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggaa
    14701 tggcacgcat ctgcctttac ggggatttgc aacgatttgg ccgccgcatc gaccttcgtg
    14761 tgaaaacggg ggctgaagcc atccgcgcac tggccacaca gctcccggcg tttcgtcaga
    14821 aactgagtga cggctggtat caggtgcgca ttgccgggcg tgatgcaggt gaaaccgaat
    14881 tgtctgcccg tcttaatgag ccgctggcaa atggtgcagt gatccacata gtaccgcgtc
    14941 tggtgggagc taaaagtggc ggtgtgtttc aggcggtgct gggggcagct gttatggcgg
    15001 ttgctatatg gatgccgggg gtaggaatta tggcgagtaa tctgctgttt tctctcggtg
    15061 ccagtatgac gcttggcggt gttgcacaga tgctggcacc gaaagccaga actccccgta
    15121 cacagacaac ggataacggc aaacagaaca cctgtttctc ctcactggat aacatggttg
    15181 cccagggcaa tgttctgcct gttctgtacg gtgaaatgcg cgtggggtcg cgggtggtat
    15241 ctcaggagat cagcacggca gacgaagggg atggtggtca ggttgtggtg attggtcgct
    15301 gatgcaaaat gtttcatgtg aaaccgcctg cgggcggttt tgtcgtttat ggagcgtgag
    15361 gaatgggtaa aggcagcagt aaggggcata ccccgcgcga agcgaaggac aacctgaaat
    15421 ccacgcagtt gctgagtgtg attgatgcca tcagcgaagg gccggttgac ggtccggtgg
    15481 atggattaaa aagcgtgctg ctgaacggta cgccggtcct ggatagcgag gggaagacaa
    15541 acttttccgg tgttacggtg gtgttccgcg ccggcgagca ggagcagaca ccgccggagg
    15601 ggtttgaatc ttccggctca gagactgtgc tgggtacgga agtgaaatac gacacgccga
    15661 tcacccggac catcacgtcg gcaaacattg atcgtctgcg cctgaccttc ggtgtgcagg
    15721 cactggtgga aaccacctca aagggggacc ggaatccgtc ggaagtccgc ctgctggttc
    15781 agatacagcg taacggtggc tgggtgacgg aaaaagacat caccattaag ggcaaaacca
    15841 cttcgcagta tctggcctcg gtggtggtgg gtaacctgcc gccgcgcccg tttaatatcc
    15901 ggatgcgcag gatgacaccg gacagcacca cagaccagct gcagaacaaa acgctctggt
    15961 cgtcgtacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcg ctggtcggcg
    16021 tgcaggtgga ttcagagcag ttcggtaacc agcaggtgag tcgcaattat catcttcgcg
    16081 ggcgcattct gcaggtgccg tcgaactata acccgcagac gcggcaatac agcggtatct
    16141 gggacggaac gcttaagcca gcatacagca acaacatggc ctggtgtctg tgggacatgc
    16201 tcactcatcc gcgctacggc atggggaaac gtcttggtgc ggcagatgtg gacaaatggg
    16261 cgctgtatgt catcggccag tactgcgacc agtcagtgcc ggacggattt ggcggcacgg
    16321 agccgcgcat cacctgtaac gcttacctga ccacacagcg taaggcgtgg gatgttctca
    16381 gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg ctgacgttcg
    16441 tgcaggaccg accgtcggat aaagtgtgga cctataaccg cagtaatgtg gtgatgccgg
    16501 atgatggcgc gccgttccgc tacagcttca gcgccctgaa ggaccgccat aatgtcgttg
    16561 aggtgaactg gattgatccg gacaacggtc atgagacggc gacagagctt gtggaggaca
    16621 cgcaggccat tgtccgttac ggtcgtaacg tcacgaagat ggatgccttt ggctgtacca
    16681 gccggggaca ggcgcaccgc gccgggctgt ggctgattaa aacggaactg ctggaaacgc
    16741 agaccgtgga cttcagcgtc ggcgcagaag ggcttcgcca tgtaccgggt gatgtcattg
    16801 aaatctgcga tgatgattat gccggtatca gcaccggtgg tcgcgtactg gcggtgaaca
    16861 gccagacccg gacgctgacg ctcgaccgtg aaatcacgct gccgtcctcc ggtaccacgc
    16921 tgataagcct ggttgacgga aatggcaatc cggtcagcgt ggaggttcag tccgtcaccg
    16981 acggcgtgaa ggtgaaagtg agccgggttc ctgacggcgt tgcagaatac agcgtgtggg
    17041 ggctgaagct gccgacgctg cgccagcgcc tgttccgctg cgtgagtatc cgtgagaacg
    17101 acgacggcac gtatgccatc accgccgtgc agcatgtgcc ggaaaaagag gccatcgtgg
    17161 ataacggggc acactttgac ggtgaccggc gcggcacggt gaatggtgtc acgccgccag
    17221 cggtgcagca tctgaccgcc gaagtcaccg cagacagcgg ggaatatcag gtgctggcgc
    17281 gatgggacac gccgaaggtg gtgaagggtg tgagcttcct gcttcgcctg accgtggcag
    17341 cggacgacgg cagtgagcgg ctggtcagca cagccaggac gacggaaacc acataccgct
    17401 tcaggcagct ggcgctgggg aattacagtc tgacagtccg ggcggtaaat gcccgggggc
    17461 agcagggcga tccggcgtcg gtatcgttcc ggattgcggc accggcagcg cctgtcacta
    17521 ttgaactgat accggggtat tttcagataa cggtggtccc gaaactggct gtatatgacc
    17581 cgacggtgca gtttgagttc tggttttcgg aaaagcggat tgcggatatc aggcaggttg
    17641 aaaccagcgc ccgctatctt ggcacggcgc tgtactggat agccgccagt atcaatatca
    17701 ggccgggcca tgattattat ttttacgttc gcagtgtgaa caccgttggc aaatcggcat
    17761 ttgtggaggc cgtcggtcgg gcgagcgatg atgcggaagg ttatctgagt ttttataaag
    17821 ggttgatcaa taaaacgcat ctcggcaagg agttgtggac gcagattgat aacggtcagc
    17881 ttgcgccgga cctgactgaa atcaggacgt ccattacgaa tgtcagcaat gaaatcacgc
    17941 aaaccgtcaa taaaaaactg gaaaatcaga gtgctgcaat ccagcagata cagaaagttc
    18001 aggttgatac aaataataac ctgaacagca tgtgggctgt gaagctgcaa cagatgaagg
    18061 acggacgcct ttatattgcg tggtatcggt gccggtattg agaatacgcc agcaggaatg
    18121 cagagtcagg tgctgctggc ggcagacagg attgcgatga ttaatcctgc gaatggcaac
    18181 acaaagccga tgtttgttgg tcagggcgat cagatattca tgaacgacgt gttcctgaaa
    18241 cgcctgacgg ctccgaccat taccagcggc ggtaatcctc cggcattttc cctgacacct
    18301 ggcggacggc tgacggcgaa aaatgccgat atcagcggta acgtgaacgc gaactccggg
    18361 acgctcaaca acgtcacgat taataagaac tgtcgggttc tgggaaaatt gtccgcgaac
    18421 cagattgaag gcgatctcgt taaaacagtg ggcaaacctt tcccacggga ctcccgggca
    18481 ccggagaggt ggccatcagg gaccattacc gtcagggttt atgacgatca gccgtttgat
    18541 cggcaaattg ttattcccgc ggtggcgttt cgcggtgcta aacatgagcg gaagaataac
    18601 aatatttatt cgtcatgccg cctgatagtg aagaaaaacg gtgctgaaat ttataaccga
    18661 acgaccctgg ataatacgct gatatatacg ggtgttattg atatgcctgc cggtcacggt
    18721 cacatgacgc tggagttttc tgtatcggca tggctggtaa atggctggta tcccacagca
    18781 agtatcagcg atttgctggt tgttgtgatg aagaaatcca cagcaggtat cagtatcagc
    18841 tgaattttat aacccagata cgggcaccag aaatggtgcc ttttttattg cagaaaagcg
    18901 agaggtaatt atgcgtaaac tttatgctgc cattttgtcc gcagccatct gtctggctgt
    18961 atccggtgcg cctgcatggg cgtctgaaca tcagtccacg ctgagcgcgg ggtatcttca
    19021 tgcctcgacg aacgctcccg gcagtgatga tctgaacggg attaacgtga aataccgtta
    19081 tgagtttacg gacacgctgg ggctggtgac gtcattcagc tatgccaacg ctgaagatga
    19141 gcaaaaaacg cattacagtg atacccgctg gcatgaggat tccgtgcgta accgctggtt
    19201 cagcgtgatg gcggggctgt ctgtgcgcgt gaatgaatgg ttcagcgcgt atgcgatggc
    19261 gggcgtggct tacagccgtg tgtcgacctt ctccggggat tatctccgcg taactgacaa
    19321 caaggggaaa acgcacgatg tgctgaccgg gagtgatgac aaccgccaca gcaacacgtc
    19381 tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa tccgtggcca ttgaccttgc
    19441 ttatgaaggt tccggcagtg gcgactggcg cactgacggt ttcatcgtgg gtgtcggtta
    19501 taagttctga ttagccaggt aacacagtgt tatgacagcc cgctggttca ggcgggcttt
    19561 ttgtggggtg aatatggcag taaagatttc aggtgtactg aaagatgggg cgggtaaacc
    19621 tgtcgtaaat tgtgcgattg aactgcgggc cagaagaacc agtccgaccg ttgtggcaca
    19681 cgttgttgcc acttgcgtga cggacaataa cggtgcttat gtgattgagg ctgagccggg
    19741 gtattacgag gttgcgcttc actgtaacgg ctggcagcca acccgtgtcg gggatattga
    19801 tgtggcaccg actgatgcac cggggacact gaacgcgttt ctgaatgcac caaaggatgg
    19861 tgatttacgt ccagaggtga tgaagcgctt tgaggaaatg gtggcgcagg cgcagcagag
    19921 tgccggggct gcagccggaa acgcacagca gacggcgcag gatgtggcgg cagccgcaac
    19981 ggcccgtgat gatgcacaac gttttgcgga gaaagcccga caggatgcaa ccgtcacagc
    20041 tgaggacaga aaggccactg cggaagatgt gacaagcaca ggagcaaatg cagccgcagc
    20101 cggacagagc gcacaggatg ccgcaggtta tgcccgcgca gcagaacagg ccaaaaatga
    20161 cattgatgct gcgctgaccg gcactctgaa aatggctaac catctgtcag aaatcgcagc
    20221 agcaggcgaa aaggcacaac agaagtcccg ggataatctg gggctgaaaa gtgcggccac
    20281 gatggaagca cagagcgaca tttacgaccg gacaaaaggc cgtctggcga tacccggcgc
    20341 attcggcttt gggtgtgctt ttctgcctga agatgttatc cgttttgaca ctaagagtga
    20401 tttcctggcc tgggtaagga atgcgctgcc agg
//
LOCUS       DAJTUN010000072        20414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTUN010000072 DAJTUN010000000
VERSION     DAJTUN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.072"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..943)
                     /gene="fbp"
                     /locus_tag="OO470_002423"
     CDS             complement(<1..943)
                     /gene="fbp"
                     /locus_tag="OO470_002423"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6251644.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVG"
     gene            1119..2492
                     /gene="mpl"
                     /locus_tag="OO470_002424"
     CDS             1119..2492
                     /gene="mpl"
                     /locus_tag="OO470_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6251645.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2575..3126)
                     /locus_tag="OO470_002425"
     CDS             complement(2575..3126)
                     /locus_tag="OO470_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6251646.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3220..4572
                     /gene="pmbA"
                     /locus_tag="OO470_002426"
     CDS             3220..4572
                     /gene="pmbA"
                     /locus_tag="OO470_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6251647.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4755..5141
                     /gene="cybC"
                     /locus_tag="OO470_002427"
     CDS             4755..5141
                     /gene="cybC"
                     /locus_tag="OO470_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6251648.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5186..5650)
                     /gene="nrdG"
                     /locus_tag="OO470_002428"
     CDS             complement(5186..5650)
                     /gene="nrdG"
                     /locus_tag="OO470_002428"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6251649.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5808..7946)
                     /gene="nrdD"
                     /locus_tag="OO470_002429"
     CDS             complement(5808..7946)
                     /gene="nrdD"
                     /locus_tag="OO470_002429"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6251650.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8340..9995)
                     /gene="treC"
                     /locus_tag="OO470_002430"
     CDS             complement(8340..9995)
                     /gene="treC"
                     /locus_tag="OO470_002430"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6251651.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10045..11466)
                     /gene="treB"
                     /locus_tag="OO470_002431"
     CDS             complement(10045..11466)
                     /gene="treB"
                     /locus_tag="OO470_002431"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6251652.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKERVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11585..12532)
                     /gene="treR"
                     /locus_tag="OO470_002432"
     CDS             complement(11585..12532)
                     /gene="treR"
                     /locus_tag="OO470_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6251653.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12717..12770
                     /gene="mgtL"
                     /locus_tag="OO470_002433"
     CDS             12717..12770
                     /gene="mgtL"
                     /locus_tag="OO470_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6251654.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12911..15607
                     /gene="mgtA"
                     /locus_tag="OO470_002434"
     CDS             12911..15607
                     /gene="mgtA"
                     /locus_tag="OO470_002434"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6251655.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15813..16199)
                     /gene="ridA"
                     /locus_tag="OO470_002435"
     CDS             complement(15813..16199)
                     /gene="ridA"
                     /locus_tag="OO470_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6251656.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16272..16733)
                     /gene="pyrI"
                     /locus_tag="OO470_002436"
     CDS             complement(16272..16733)
                     /gene="pyrI"
                     /locus_tag="OO470_002436"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6251657.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16746..17681)
                     /gene="pyrB"
                     /locus_tag="OO470_002437"
     CDS             complement(16746..17681)
                     /gene="pyrB"
                     /locus_tag="OO470_002437"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6251658.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17685..17819)
                     /gene="pyrL"
                     /locus_tag="OO470_002438"
     CDS             complement(17685..17819)
                     /gene="pyrL"
                     /locus_tag="OO470_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6251659.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18100..18495)
                     /locus_tag="OO470_002439"
     CDS             complement(18100..18495)
                     /locus_tag="OO470_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6251660.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18627..19340)
                     /gene="bdcA"
                     /locus_tag="OO470_002440"
     CDS             complement(18627..19340)
                     /gene="bdcA"
                     /locus_tag="OO470_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6251661.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19411..20004
                     /locus_tag="OO470_002441"
     CDS             19411..20004
                     /locus_tag="OO470_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6251662.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20149..>20414
                     /gene="tabA"
                     /locus_tag="OO470_002442"
     CDS             20149..>20414
                     /gene="tabA"
                     /locus_tag="OO470_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6251663.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGA"
BASE COUNT         4850 a         5152 c         5407 g         5005 t
ORIGIN      
        1 tgccgacaaa gaacgaacgg cgctggtgca gagtttccgg gatgatatcc agaatacgct
       61 ctttgccatc gctcgcttta ccgcccgctt gttccgccag gaatgccatc gggttgcact
      121 cataaagcaa acgcagtttg ccgtccgggt ggctggcggt gcttgggtag agataaatac
      181 cgcctttcag caggttacgg tggaaatccg caaccagtga accgatataa cgcgaggtat
      241 acgggcgatt ggtggattta tcttcttcct ggcagaattt aatgtacttc ttcaccccgt
      301 tcggaaactt aatgtagttt ccttcgttga tggagtaggt tttgcctttc tccgggaagc
      361 gcatccgttc ctggcacagg cagaaaacgc ccagcgaagg atcgtaagta aaggcgtgaa
      421 caccgcatcc ggtggtgtaa accagcatgg tagaggagcc gtataccacg taacctgccg
      481 caacctgttt gttaccaggc tggaggaaat cttcttccgt taccggcgtg ccaacaggcg
      541 taacgcggcg gtagatggag aaaatggtac cgacagagac gttaacatcg atgttggacg
      601 agccatccag ggggtccatc agcaccacgt attttgcgtg ttcacagcct tcaaagacga
      661 caatctcatc ttcttcttca gaggcaatcc ccgcaacgat atcgcgtgct ttcagtgcgg
      721 ctttcagttt ttcattagcg aacaagtcga gtttttgctg aacctcgccc tgcacgttct
      781 cagcaccgct ggcacccagg atatcaacca gtcctgcttt gttgatatcg cgatggataa
      841 tcttggcgcc cagttttatt gccgacagca aagcagtgag ctcaccggta gcatgagaaa
      901 actcgtgctg cttttcgaca ataaattcac ctaacgtttt cataaaactt tccctgcaat
      961 gtttatggag taaagcgacc gcaacaatct taacaaataa tctcaatgtt gcgctcaggt
     1021 gaatcgcgcc agcaaattgc ggattatcct gaaatgcgtt tctcacttgc ccgacatatg
     1081 cgtaaaatga gcggcagatt aaaaaaggat agtgacgtat gcgcattcat attttaggaa
     1141 tttgtggcac atttatgggc ggtctggcga tgctggcgcg ccagttaggc catgaagtaa
     1201 cgggttcgga cgccaatgtg tatccgccga tgagcacctt acttgagaag caaggcattg
     1261 agctgattca gggttacgat gccagccagc tcgagccgca gccggatctg gtgattattg
     1321 gcaacgccat gacccgtgga aatccgtgtg tggaagcggt actggaaaaa aacatccctt
     1381 atatgtcagg tccacagtgg ctgcacgatt ttgtgctgcg cgaccgctgg gtgctggccg
     1441 ttgccggtac acacggcaaa accaccaccg cgggaatggc gacctggatt ctggaacagt
     1501 gtggttacaa accgggattt gtgatcggcg gtgtgccggg gaactttgag gtttcggcgc
     1561 gtctgggcga aagcgacttc tttgttatcg aagcggatga gtacgactgc gccttcttcg
     1621 acaaacgctc taaatttgtc cattactgcc cgcgtacgct gatcctcaac aaccttgagt
     1681 tcgatcacgc cgatatcttt gacgacctga aagcgatcca gaaacagttc caccatctgg
     1741 tgcgtatcgt tccggggcag ggccgtatta tctggccgga aaatgacatc aacctgaaac
     1801 agaccatggc gatgggctgc tggagcgagc aggagctggt gggtgagcag ggtcactggc
     1861 aggcgaaaaa gctgaccacc gatgcttccg aatgggaagt tttgctggat ggcgaaaaag
     1921 tgggcgaagt gaaatggtcg ctggtaggcg aacataatat gcacaatggc ctgatggcga
     1981 ttgcagcggc tcgccatgtt ggtgtagcgc cggcagatgc cgctaacgcg ctgggttcgt
     2041 ttattaatgc tcgtcgccgt ctggagttgc gtggtgaagc gaatggcgtc acggtatatg
     2101 acgattttgc ccatcacccg acggcgattc tggcaacgct ggcggcgctg cgtggcaaag
     2161 ttggtggtac ggcgcgcatt attgctgtgc tggaaccgcg ctcgaatacc atgaaaatgg
     2221 ggatctgcaa agacgatctg gcaccttcat taggtcgtgc cgatgaagtc ttcctgctgc
     2281 aaccggcgca tattccgtgg caggtggcag aagtggcaga agcctgcgtt cagcctgcac
     2341 actggagtgg cgatgtggat acgctggcag atatggtggt gaaaaccgct cagcctggcg
     2401 accatattct ggtgatgagc aacggcggtt ttggtgggat ccatcagaaa ctgctggatg
     2461 gtctggcgaa gaaggcggaa gctgcgcagt aattcggctt cagcctgata tagcattgcc
     2521 ggataaggcg tttacgccgc atccggcaaa ccatactcac atcaacaacg aaaattaccc
     2581 ttcattctcc gccagttcgc gcagatactg gaaaatctgg cgtgcggatt tcggcggctt
     2641 attcccttct ttctctttct tcgcgttgcg gatcagtgta cgcagttgct gacgatcggc
     2701 atcgggccac agattcaata cttcagcgat ggcgtcatca ccttgatcga tcagacgatc
     2761 gcgcaggttt tccagtttat ggaacagcac cacctgctgg ttgtgacggt ttttcagctt
     2821 atccagcgcc tgacgaatag gctctacgtc gcgctggcgc agcatcttac cgatgagctg
     2881 caactggcgg cggcgacctt ccatcttaat acgctgggcg agctcaatag ccgggcgcag
     2941 atccgcatct aacgggatct tatccagcgc gtttttcccc agatcaacaa tttccgcgcc
     3001 aaggcgtttt agctcctcgg catcacgttt aatttcactt ttactgaccc agataatttc
     3061 atcgtcttcg tcttcgatgt catcaccggg aacgtcgtcg agccagtctt cgggctgctt
     3121 agtcatctca ggctccttaa aaaaagaggc taatgttacc agttaagatg cgcactgaaa
     3181 aacggttctc tgttagactt cagagaaact ctctacatta tggcacttgc aatgaaagta
     3241 atctctcaag ttgaagcgca gcgcaagatt ctggaagaag cagtttccac tgcgctggag
     3301 ttggcctcag gcaaatcgga cggtgcggaa gttgccgtca gcaagaccac cggcattagc
     3361 gtaagcacgc gttatggtga agtggagaat gttgaattca atagtgatgg cgcgctgggg
     3421 atcactgttt atcaccagaa ccgtaaaggt agcgcatcgt ctaccgattt aagcccgcag
     3481 gccattgccc gcaccgtaca ggcggcgctg gatattgccc gttatacctc gccagatccc
     3541 tgtgccggcg tggcagacaa agagctgctg gcctttgacg caccgaatct cgacttgttc
     3601 caccctgcgg aagtttcccc ggatgaagcc attgaactgg cggcccgcgc agaacaggcg
     3661 gcattgcagg cggacaaacg catcaccaat accgaaggtg gcagctttaa cagccactac
     3721 ggtgtcaaag tttttggcaa cagccacggc atgttgcagg gttactgctc aacgcgtcat
     3781 tcgctctcca gctgtgtaat tgccgaagaa aatggcgata tggagcgtga ttacgcctac
     3841 accattggcc gcgcgatgag cgatctgcaa acgccagagt gggtcggggc cgactgtgct
     3901 cgccgtactt tatcgcgcct gtcaccgcgt aaactctcca ccatgaaagc gccggtcatt
     3961 tttgccaatg aagtggcaac cgggcttttt ggccatctgg tgggggcgat agcgggtggc
     4021 tcggtttacc gtaaatctac ctttctgctg gattcgctgg gtaaacaaat tctgccggac
     4081 tggctgacca ttgaagagca tccgcatctg ctgaaagggc tggcgtcgac gccattcgac
     4141 agcgaaggtg tgcgcaccga gcgtcgcgat attattaaag atggcatcct gactcagtgg
     4201 ctgctgacca gctactcggc gcggaaactg gggctgaaaa gcaccggaca tgcgggcggt
     4261 attcacaact ggcggattgc cggacaaggg ctaagcttcg aacaaatgct caaagagatg
     4321 ggcaccgggc tggtggtgac ggagctgatg ggccagggcg tgagtgccat taccggtgat
     4381 tattcccgtg gtgcagcggg cttctgggta gagaatggcg aaattcagta tcccgttagt
     4441 gaaatcacca tcgcaggtaa tttaaaagat atgtggcgca atattgtcac cgtcggtaac
     4501 gatattgaaa cacgcagtaa tatacagtgt ggttctgtgc tgttgccgga gatgaaaatc
     4561 gccgggcagt aaaaaactgg cgcgacctgc cgcgctataa actaaaaaat tacacaaaat
     4621 cattcgcact gcatcgagac ggaaagtgaa tgagggccgg ggagcgtact cgcagtacgt
     4681 gacccgggcg aatgagcgta gccgtcgatg aggcagtgtg aaggatgaag tgtaaataaa
     4741 aaaggaagtg agcaatgcgt aaaagcctgt tagctattct tgcagtctcc tcgttggtat
     4801 tcagttctgc gtcgtttgcc gctgatcttg aagacaatat ggaaaccctc aacgacaatt
     4861 taaaagtgat cgaaaaagcg gataacgcgg cgcaagtcaa agacgcgtta acgaagatgc
     4921 gcgccgcagc gctggatgcg caaaaagcaa cgccgccgaa gctcgaagat aaatcaccgg
     4981 acagcccgga aatgaaagat ttccgccacg gtttcgacat tctggtcggt cagattgacg
     5041 acgcgctgaa gctggcaaat gaaggtaaag taaaagaagc gcaggctgct gcagagcaac
     5101 tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt tccctgttgc
     5161 ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga ttgctgctgc
     5221 cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg accagcacgt
     5281 taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta tagcctgtcc
     5341 acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt ttcagaatat
     5401 ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc tggcgtttga
     5461 tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta aatggctgac
     5521 cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga acacacccgg
     5581 agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga tagtactgat
     5641 gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc ggtaatcaat
     5701 gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat gtaggccgga
     5761 taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta acctatctgc
     5821 ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc gttaaacgga
     5881 cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac acgggaggcg
     5941 tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa ctcaccggta
     6001 aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg tacatgctga
     6061 tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt tggatactcg
     6121 ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa gtcgatcttg
     6181 tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta gtaaccttta
     6241 tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa gcggtcgcac
     6301 aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc tttccactga
     6361 tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag cttctcgttg
     6421 tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc gatgtaaccc
     6481 agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc agcattcaga
     6541 cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt cacgccttcg
     6601 agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc atccagcagc
     6661 ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag gttcaggctg
     6721 atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt ttcccacacg
     6781 ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt cactacctga
     6841 tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc cagctgtttg
     6901 atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc aaacaccagt
     6961 ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt acgcaggatc
     7021 gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa ggttacaaac
     7081 ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga ctggaaggca
     7141 tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg gatgttccac
     7201 tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc cagcacttca
     7261 tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc aataatctgc
     7321 gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc gttccccatt
     7381 ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa catcgggaag
     7441 aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc ctgcaccacg
     7501 tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc cagcaggtcg
     7561 cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa cgaggcgttg
     7621 gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc acgttcaatg
     7681 tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg acccgacatc
     7741 agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg cccctgcatc
     7801 tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac ttccgctgct
     7861 ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac tttgcagccg
     7921 tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca ggttatccac
     7981 agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct ttccgcaatc
     8041 gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta gcaattatag
     8101 tcgattaata caacatattg ggttgggatg cattttaaag tctatatata gtgctttgca
     8161 tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc gtacggatga
     8221 cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa atcaacaaaa
     8281 acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt agggaagatt
     8341 tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca gggttgtggt
     8401 gaggcttctt cgtaattatg catcacaagc tgccagttgc cgcgcatttg ccctggctgc
     8461 cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc cttccattca
     8521 cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt gccccatgtc
     8581 aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa cacagaggag
     8641 tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag gccaatccac
     8701 ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg cgtgcggctg
     8761 ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg accatcgttg
     8821 cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat gcgcgtgaaa
     8881 tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc ctgcatgcca
     8941 tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc atcaccaaag
     9001 cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca tgccacgttg
     9061 tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc aggtttagcc
     9121 agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt aaaggtcatc
     9181 gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct ggtggaggac
     9241 atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat ctcgtgcaaa
     9301 aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc caggtcttca
     9361 gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc gtcgacccca
     9421 cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg attctcccag
     9481 ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct ttccgcatgc
     9541 cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt ttctggttcg
     9601 ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc ctcgcgaaac
     9661 caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg aatcccgcgc
     9721 gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt gggatcaatc
     9781 gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata aaagggggtt
     9841 agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat aacgccacgt
     9901 aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat ctggtagata
     9961 acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca aatgcgaggg
    10021 ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc ggtatttccg
    10081 ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca ttgccagcgc
    10141 aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc ctacgccgat
    10201 accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag aaccaatcat
    10261 cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag gctcagtgac
    10321 cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt gcttgcggct
    10381 ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg caatcagcgg
    10441 ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa gggtggtctg
    10501 gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg caccaatcgg
    10561 agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc caatcatgcg
    10621 accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg aacagacggg
    10681 caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt caataacgcc
    10741 cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca ctttggcgat
    10801 gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat aagcgttcat
    10861 cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg tgccgcccat
    10921 ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg cttcaccgat
    10981 caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca gcgtctgacc
    11041 gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac cgccgctaat
    11101 caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact gctcatgcca
    11161 tttcatattt tgccgggcgg ctttttttac ccgctcttta tcaacctgcg cctgtccggt
    11221 tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca cctgaaattg
    11281 cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt tcggtctggc
    11341 attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca ccgtcgcaat
    11401 attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt ttattttgct
    11461 catcataaag ccccatggca gatgacattt ttggttggct gcagaatata ttgcgcggaa
    11521 aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat cgttcaggaa
    11581 acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc ccggttacct
    11641 gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga tctacggtga
    11701 cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc agttgcaagg
    11761 tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg tcggttgcgc
    11821 acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag ccttgcttca
    11881 tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag aacgccaggt
    11941 aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg agataactga
    12001 tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc ccttcgtcgt
    12061 cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc gatgactgcc
    12121 agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg ccgtcgatat
    12181 tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac tgattttcca
    12241 tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga acggcgagat
    12301 tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg ctttgcccac
    12361 gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact gcttcaacac
    12421 gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa accgtagatt
    12481 tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc atcctgttgt
    12541 cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca gcaatgggta
    12601 aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata aatgctacga
    12661 atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga ggttatatgg
    12721 aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa gcccgtcttt
    12781 tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc agctttatta
    12841 ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag acagtgcgga
    12901 gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac cttcccgtct
    12961 ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg tcccgccgtc
    13021 cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt ggaaaacgtt
    13081 cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg aacaacatgg
    13141 tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat gggtctgcta
    13201 tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg ccacggaaga
    13261 tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc tgaactttat
    13321 tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca gtaataccgc
    13381 gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga tcccgataga
    13441 ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc cggcagattt
    13501 acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg gtgagtctct
    13561 gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc tggagtgcga
    13621 caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa tggtgattgc
    13681 tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc aggaaagcga
    13741 gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc gctttatgct
    13801 ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact ggtgggaagc
    13861 ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc cgatgattgt
    13921 tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga tcgtcaaaca
    13981 tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata aaaccggcac
    14041 cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta aaaccagcga
    14101 acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta aaaatctgct
    14161 tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg ccagccgttg
    14221 gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg tggtagtggc
    14281 agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa tcctcaatgt
    14341 gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca tgctgcgtaa
    14401 gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg cggtggcgac
    14461 gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg acctgatcct
    14521 cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg cactgaaggc
    14581 attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt tagttgcagc
    14641 gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta gtgatattga
    14701 aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt ttgcccgcct
    14761 gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc atgtggttgg
    14821 ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata tcggcatttc
    14881 tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc tggaaaaaag
    14941 cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca acatgctgaa
    15001 atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc tggtggcgag
    15061 tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga acctgctgta
    15121 cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa ttcaaaagcc
    15181 gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac cgatcagctc
    15241 gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca acacgccgga
    15301 aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc aaacgctgat
    15361 tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat cgtggccgtt
    15421 aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt caccgctggc
    15481 cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg ttgcgattct
    15541 ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc gttacggctg
    15601 gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa agtgagcttt
    15661 gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata aattgcagga
    15721 atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca tcagacttaa
    15781 tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat tgcttcgatc
    15841 tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc cgggaaagtg
    15901 gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa gtcgttcaga
    15961 tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc ggcttcgacg
    16021 attgctttta cgttatccag cgactgacgt gcctgtgcag cgacgtctgc cggtacttca
    16081 ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc cagatcaacg
    16141 ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt tttgctcatg
    16201 atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca gacttttatt
    16261 accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc acagtatttg
    16321 catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga aaccggttcg
    16381 gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg ctctggcaga
    16441 cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt ggcttgcggt
    16501 gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc gattttgatc
    16561 agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat gcgctgatct
    16621 gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg gatatggtca
    16681 attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt catctctatt
    16741 tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg gcgagcgaaa
    16801 atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac atccgtcgca
    16861 atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc gttgtggaga
    16921 tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg gtccagacgc
    16981 tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc ttcaatagag
    17041 ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta ttgcggcatt
    17101 gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc tagcgcctgg
    17161 gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac gtggagattg
    17221 tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg cgtcggatgt
    17281 tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc ggtggctagg
    17341 cgcgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt gctgataacc
    17401 gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc gctgtcggag
    17461 aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag gcgggtacgg
    17521 gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc tggttgcggg
    17581 tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg actaaggtcg
    17641 tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct cctgacacct
    17701 aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg gaagaaaaac
    17761 ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca ctgaaccata
    17821 catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc aagcatttga
    17881 tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata tatgcatttt
    17941 tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca gacatttcct
    18001 gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt catccgactt
    18061 atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag ggatacgcgc
    18121 tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg ccgtccagtt
    18181 tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt caaattgttt
    18241 ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc atcccgcagc
    18301 tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa aatcgggttc
    18361 tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat cgccggaacg
    18421 aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag ggaaaacagc
    18481 ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag atttatattt
    18541 atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat catggataat
    18601 ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat ggtatgcatc
    18661 gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc agcgacctct
    18721 tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg cattggcccg
    18781 ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat ggtgatccct
    18841 cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga tttactggcg
    18901 gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc gatgattaag
    18961 atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata aggagcatga
    19021 atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc atcgccaaag
    19081 acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg aacgacatca
    19141 ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt ctcttgtgcc
    19201 agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc cccatcggtg
    19261 acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat gagaactgtc
    19321 ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc attacaaaac
    19381 tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc tcgcgttcca
    19441 ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt gctttttcac
    19501 caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg tattaacccc
    19561 ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt actcaatgaa
    19621 tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg tccggtaggc
    19681 gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa cggcggctgc
    19741 gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg tgatattgcc
    19801 attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag gcggcatcca
    19861 caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct ttctgcgaag
    19921 gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc aggggaagcg
    19981 ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga acgcgcctgc
    20041 cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg atcatctttt
    20101 cgtataacga tagaaatgaa acattatttt aattaaggag tggaaaagat gatcatcgga
    20161 aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat tgagcatatc
    20221 aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg caatcgtctg
    20281 ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc ggagtaccat
    20341 gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac cttcagcacg
    20401 caacctgcag gcgc
//
LOCUS       DAJTUN010000073        20304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTUN010000073 DAJTUN010000000
VERSION     DAJTUN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.073"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            81..422
                     /gene="raiA"
                     /locus_tag="OO470_002443"
     CDS             81..422
                     /gene="raiA"
                     /locus_tag="OO470_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6251664.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    525..650
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            526..573
                     /gene="pheL"
                     /locus_tag="OO470_002444"
     CDS             526..573
                     /gene="pheL"
                     /locus_tag="OO470_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6251665.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            672..1832
                     /gene="pheA"
                     /locus_tag="OO470_002445"
     CDS             672..1832
                     /gene="pheA"
                     /locus_tag="OO470_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6251666.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1875..2996)
                     /gene="tyrA"
                     /locus_tag="OO470_002446"
     CDS             complement(1875..2996)
                     /gene="tyrA"
                     /locus_tag="OO470_002446"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6251667.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3007..4077)
                     /gene="aroF"
                     /locus_tag="OO470_002447"
     CDS             complement(3007..4077)
                     /gene="aroF"
                     /locus_tag="OO470_002447"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6251668.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4287..4652
                     /locus_tag="OO470_002448"
     CDS             4287..4652
                     /locus_tag="OO470_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6251669.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4802..5320
                     /locus_tag="OO470_002449"
     CDS             4802..5320
                     /locus_tag="OO470_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6251670.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5310..6535
                     /gene="dgcN"
                     /locus_tag="OO470_002450"
                     /pseudo
     CDS             5310..6535
                     /gene="dgcN"
                     /locus_tag="OO470_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6551..7033
                     /locus_tag="OO470_002451"
     CDS             6551..7033
                     /locus_tag="OO470_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6251671.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7110..7457)
                     /gene="rplS"
                     /locus_tag="OO470_002452"
     CDS             complement(7110..7457)
                     /gene="rplS"
                     /locus_tag="OO470_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6251672.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7499..8266)
                     /gene="trmD"
                     /locus_tag="OO470_002453"
     CDS             complement(7499..8266)
                     /gene="trmD"
                     /locus_tag="OO470_002453"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6251673.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8297..8845)
                     /gene="rimM"
                     /locus_tag="OO470_002454"
     CDS             complement(8297..8845)
                     /gene="rimM"
                     /locus_tag="OO470_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6251674.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8864..9112)
                     /gene="rpsP"
                     /locus_tag="OO470_002455"
     CDS             complement(8864..9112)
                     /gene="rpsP"
                     /locus_tag="OO470_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6251675.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9249..10610)
                     /gene="ffh"
                     /locus_tag="OO470_002456"
     CDS             complement(9249..10610)
                     /gene="ffh"
                     /locus_tag="OO470_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6251676.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10777..11568
                     /locus_tag="OO470_002457"
     CDS             10777..11568
                     /locus_tag="OO470_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6251677.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11589..12875
                     /locus_tag="OO470_002458"
     CDS             11589..12875
                     /locus_tag="OO470_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6251678.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12930..13523)
                     /gene="grpE"
                     /locus_tag="OO470_002459"
     CDS             complement(12930..13523)
                     /gene="grpE"
                     /locus_tag="OO470_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6251679.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13646..14524
                     /gene="nadK"
                     /locus_tag="OO470_002460"
     CDS             13646..14524
                     /gene="nadK"
                     /locus_tag="OO470_002460"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6251680.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14610..16271
                     /gene="recN"
                     /locus_tag="OO470_002461"
     CDS             14610..16271
                     /gene="recN"
                     /locus_tag="OO470_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6251681.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16420..16761
                     /gene="bamE"
                     /locus_tag="OO470_002462"
     CDS             16420..16761
                     /gene="bamE"
                     /locus_tag="OO470_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6251682.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16823..17113)
                     /locus_tag="OO470_002463"
     CDS             complement(16823..17113)
                     /locus_tag="OO470_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6251683.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17103..17540)
                     /gene="ratA"
                     /locus_tag="OO470_002464"
     CDS             complement(17103..17540)
                     /gene="ratA"
                     /locus_tag="OO470_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6251684.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17711..18193
                     /gene="smpB"
                     /locus_tag="OO470_002465"
     CDS             17711..18193
                     /gene="smpB"
                     /locus_tag="OO470_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6251685.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18408..18770
                     /gene="ssrA"
                     /locus_tag="OO470_002466"
     tmRNA           18408..18770
                     /gene="ssrA"
                     /locus_tag="OO470_002466"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18975..20216
                     /locus_tag="OO470_002467"
     CDS             18975..20216
                     /locus_tag="OO470_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6251686.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5237 a         5317 c         4774 g         4976 t
ORIGIN      
        1 tatgagattt ccatcacaca ttttgacatc aggaacggta tgctgaattc accaagacgg
       61 gaagacaaga ggtaaaattt atgacaatga acattaccag caaacaaatg gaaattactc
      121 cggccatccg ccaacatgtc gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc
      181 tgattaatcc acatatcatt ctgtccaaag agccacaagg gtttgttgct gacgccacaa
      241 tcaatacacc taacggcgtt ctggttgcca gtggtaaaca tgaagatatg tacaccgcaa
      301 ttaacgaatt gatcaacaag ctggaacggc agctcaataa actgcagcac aaaggcgaag
      361 cacgtcgtgc cgcaacatcg gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt
      421 agtcctttat attgagtgta tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt
      481 gaaaacagta cgggtactgt actaaagtca cttaaggaaa caaacatgaa acacataccg
      541 tttttcttcg cattcttttt taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca
      601 gaatgcgaag acgaacaata aggcctccca aatcgggggg ccttttttat tgataacaaa
      661 aaggcaacac tatgacatcg gaaaacccgt tactggcgct gcgagagaaa atcagcgcgc
      721 tggatgaaaa attattagcg ttactggcag aacggcgcga actggccgtc gaggtgggaa
      781 aagccaaact gctctcgcat cgcccggtac gtgatattga tcgtgaacgc gatttgctgg
      841 aaagattaat tacgctcggt aaagcgcacc atctggacgc ccattacatt actcgcctgt
      901 tccagctcat cattgaagat tccgtattaa ctcagcaggc tttgctccaa caacatctca
      961 ataaaattaa tccgcactca gcacgcatcg cttttctcgg ccccaaaggt tcttattccc
     1021 atcttgcggc gcgccagtat gctgcccgtc actttgagca attcattgaa agtggctgcg
     1081 ccaaatttgc cgatattttt aatcaggtgg aaaccggcca ggccgactat gccgtcgtac
     1141 cgattgaaaa taccagctcc ggtgccataa acgacgttta cgatctgctg caacatacca
     1201 gcttgtcgat tgttggcgag atgacgttaa ctatcgacca ttgtttgttg gtctccggca
     1261 ctactgattt atccaccatc aatacggtct acagccatcc gcagccattc cagcaatgca
     1321 gcaaattcct taatcgttat ccgcactgga agattgaata taccgaaagt acgtctgcgg
     1381 caatggaaaa ggttgcacag gcaaaatcac cgcatgttgc tgcgttggga agcgaagctg
     1441 gcggcacttt gtacggtttg caggtactgg agcgtattga agcaaatcag cgacaaaact
     1501 tcacccgatt tgtggtgttg gcgcgtaaag ccattaacgt gtctgatcag gttccggcga
     1561 aaaccacgtt gttaatggcg accgggcaac aagccggtgc gttggttgaa gcgttgctgg
     1621 tactgcgcaa ccacaatctg attatgaccc gtctagaatc acgcccgatt cacggtaatc
     1681 catgggaaga gatgttctat ctggatattc aggctaatct tgaatcagcg gaaatgcaaa
     1741 aagcattgaa agagttaggg gaaatcaccc gttcaatgaa ggtattgggc tgttacccaa
     1801 gtgagaacgt agtgcctgtt gatccaacct gatgaaaagg tgccggatga tgtgaatcat
     1861 ccggcactgg attattactg gcgattgtca ttcgcctgac gcaataacac gcggctttca
     1921 ctctgaaaac gctgtgcgta atcgccgaac cagtgctcca ccttgcggaa actgtcaata
     1981 aacgcctgct tatcgccctg ctccagcaac tcaatcgcct cgccgaaacg cttatagtaa
     2041 cgtttgatta acgccagatt acgctctgac gacataatga tgtcggcata aagctgcgga
     2101 tcctgagcaa acagtcgccc gaccatcgcc agctcaaggc ggtaaatcgg cgaagagagc
     2161 gccagaagtt gctcaagctg aacattttct tctgccaggt gcagcccgta agcaaaagta
     2221 gcaaagtggc gcagagcctg aataaacgcc atattctgat cgtgctcgac agcgctaata
     2281 cgatgcaacc gagcgcccca gacatgaatt tgctccagaa accattggta tgcttccggc
     2341 ttacgtccat cacaccagac cacaacttgc tttgccaggc taccgctgtc cgggccgaac
     2401 atcgggtgta accccagtac cgggccatcg tgcgccgcca gcatggcctg taatggtcca
     2461 tttttcactg atgccagatc aaccagaata caatctttcg gtaaaggcgg taatttgccg
     2521 ataacttgct cagtaacgtg gattggcaca ctaacaatca ccattccggc atcggcaaca
     2581 atatcagccg ctcgatccca gtcatgttgc tccagaatcc gcacctgata acccgatagt
     2641 gtcagcatct tctcgaacag gcgtcccatc tgaccgccac cgccgacgat aaccaccgga
     2701 cgcagtgacg gacaaagtgt tttaaatcct ttgtcgtttt cactggagta agattcacgc
     2761 atcacccgac gcaaaacatc ctcaatcaga tctggcggta cacccagagc ttccgcctct
     2821 gcacgacgcg aggccaacat agatgcctcg cgctccggaa cataaatagg cagtccaaag
     2881 cggcttttca ccttgcccac ttcagcaacc agttccagac gcttcgctaa taaattcagc
     2941 agcgctttat cgacttcatc aatttgatcg cgtaatgcgg tcaattcagc aaccataata
     3001 aacctcttaa gccacgcgag ccgtcagctg cccgttcaga tcctgatgaa tttcacgcag
     3061 caaggcatcg gtcatttccc agctaatgca ggcatcggtt acggatacac cgtatttcat
     3121 ttcactgcgc ggttgctcag aagactgatt gccctcgtgg atattacttt cgatcatcag
     3181 accaataatt gagcgattac catctttgat ttgagcaacc acagattctg ccaccgcagg
     3241 ctgacggcga taatctttat tggaattacc gtggctgcaa tctaccatca gagacgggcg
     3301 cagtcccgcc tgttccatct ctttttcaca ttgcgcaaca tccgcagggc tatagttcgg
     3361 cgctttacca ccgcgcagga tcacatgccc gtccggattc ccctgagttt gtagcaacgc
     3421 aacctgccct gcctggttaa tgccaacaaa acagtgcggc tgggcggcgg cgcgcatagc
     3481 gttaattgct gttgccagac tgccgtcggt gccgttttta aaaccaaccg gcatggaaag
     3541 cccggaggcc atttcacggt gagtttgcga ttccgttgta cgagcaccaa ttgctgacca
     3601 gctaaacaga tcgcccaggt attgcgggct attcggatct aacgcttccg tcgccagtgg
     3661 cagtcccata ttcaccagct caagcaacaa tttacgcgcg atctgcagcc cggcttctac
     3721 atcaaaagag ccatccatat ggggatcgtt aattaaccct ttccagccga cagtggtacg
     3781 gggtttttca aaatagacgc gcattaccag atagaggcta tcgctgacct ctgcggcaag
     3841 agctttaaat cgacgagcat attccagagc agtttccgga tcatgaatgg aacaaggacc
     3901 acatactacc agcagacgag gatcgcgccc ggcgataata tctgaaatgg ttttacgcga
     3961 gtcagcaatc tgggcttctt gttgcaggct caatggaaaa gcggccttca gttgttccgg
     4021 agtcattaaa acctgttcgt cggtaatatg tacgttattc agcgcgtctt tatgcatgat
     4081 ggcgatcctg tttatgctcg tttgcgatag ttgctcctca gcgaggatga cgtaacgata
     4141 acacataaag taaagttttc aatccatatt tcgtacattt ttatttacac agacaattta
     4201 gtcgcgcttt caacccttgc ttctgtatag ataaatttac attcccttta aaaacaatcc
     4261 gctatgcttt gaaaaaggag aaagaaatga tgaaaaagtt tatcgccccc ttgttggctt
     4321 tactggttag cggatgtcag attgatcctt atactcacgc gccaaccttg accagcaccg
     4381 actggtatga tgtcggtatg gaagatgcga tatcgggcag cgccataaaa gatgacgatg
     4441 catttagcga ttcacaggcg gatcgcggtc tataccttaa aggatatgcc gaagggcaaa
     4501 agaaaacttg ccagaccgat tttacctatg cccggggact ttccggtaaa agctttcctg
     4561 ccagctgcaa caatgttgaa aatgccagcc aactgcatga agtatggcaa aaaagggctg
     4621 atgaaaatgc cagcacaata cgattaaatt aattaaaacc ccacaaataa attaaacata
     4681 agattttact cattgtgttt tatttctcac attgatgacg gtcgcatgaa aaaatgatat
     4741 tttaatgagc atcctgaaaa aatatcgatg catttcgagc gaagatggtg gggatccctg
     4801 aatgcgtttt tctcaccgac tgttcttact tctgattctc ctgctgacgg gagcaccaat
     4861 actcgctcaa gagccttctg acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt
     4921 cagttacacc cgctggcctg cgctatcagg gccaccaaag ttatgtatct tttcatcttc
     4981 acgttttagt accgccctac aagagaatgc cgcgacatct ttgccttatc tgcctgtcat
     5041 cattcacact caacaagagg cgatgatttc aggctgcaat ggtttttatt ttggcaacga
     5101 gtcaccgaca ttccagatgg aattaaccga acaatatccg tcaaaggcgt tattattaat
     5161 tgccgaacaa aataccgagt gcattattgg tagcgccttt tgtctgatca tccacaataa
     5221 tgacgtcaga tttgccgtaa acctggatgc ctcatcgcgt agcggtgtaa aggtcaaccc
     5281 ggatgtctta atgctcgcac ggaagaaaaa tgatggataa cgataattct cttaataagc
     5341 gccccacgtt taaaagagca ttacgcaaca tcagtatgac cagcatattt atcactatga
     5401 tgctgatctg gttgctgctt tccgtgacct cggtgctgac cctgaaacag tacgcgcaaa
     5461 aaaacctggc actgacagca gcaacaatga cttacagtct ggaagcagct gtcgtttttg
     5521 ccgatggccc tgcagcaact gaaacactgg cagcgctggg ccagcaaggg cattttcaac
     5581 tgcagaagta cgtgataagc agcaaaatat tctggcatcc tggcattacc cccgtaagga
     5641 tccaggcgat actttcagca atttcataag ccactggctc ttccctgccc ccatcattca
     5701 gccgattcgt cacaatggtg aaaccattgg cgaagtacgc ttaaccgctc gcgacagttc
     5761 aatcagccat tttatctggt tttcgctcgc cgtactgacc ggttgtattc tgctggcatc
     5821 aggcatcgca attaccctca cccgccattt gcacaatggc ctggtggaag cactgaaaaa
     5881 tatcaccgat gtcgtacatg atgtgcgttc caaccgcaat ttttcccgac gagtttcgga
     5941 agaacgtatc gctgagtttc accgcttcgc tctcgacttc aacagtctgc tggatgaaat
     6001 ggaagagtgg cagcttcgtt tacaggccaa aaatgcgcag cttctgcgta ccgcgctaca
     6061 tgacccatta accgggctgg ctaaccgcgc agcgtttcgt agcggcatca atacgttgat
     6121 gaacaattcc gatgcccgaa aaacgtcggc gttactattt cttgatggcg ataatttcaa
     6181 atatatcaat gatacctggg gtcatgcgac gggcgatagg gtcttgattg aaatcgcaaa
     6241 acggttagct gaatttggcg ggctgcgaca taaagcatac cgcctgggcg gcgatgaatt
     6301 cgctatggtg ctctatgatg tacagtcgga atctgaagtg cagcagatat gctcagcact
     6361 gacacaaatc tttaatctcc cgtttgatct tcataatggc catcagacca ccatgacatt
     6421 aagcattggt tacgcgatga ccattgagca cgcctctgcg gaaaaattac aagagcttgc
     6481 cgatcacaat atgtatcagg ccaaacacca gcgtgccgaa aagctggtga gataacaagg
     6541 atatatcgat atgataaagc acctggtagc acccctgatt ttcacctcac taatactgac
     6601 tggctgccag tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta
     6661 tggatttact gaatccgccg gcgactggtc gctgggctta tcagatgcca ttctgttcgc
     6721 aaaaaatgac tacaaattgc tcccggaaag ccagcaacag atccaaacca tggcagccaa
     6781 attggcctcg acagggctaa cacatgcccg tatggatgga cacaccgata actatggtga
     6841 agacagttac aacgaaggct tatcattgaa acgggcgaat gtcgtggccg atgcatgggc
     6901 tattggtgga caaattccac gcagcaatct caccacacag ggtttaggaa aaaaatatcc
     6961 catagccagt aacaagaccg cccagggccg cgccgagaac cgccgcgtcg cagtggtgat
     7021 tacaacccct taaaacacga gcaaacagac aaaaaaaggg ccagccaatt ggccagccct
     7081 tcttaacagg atgtcgctta agcgaaatct tagttaagac gctctttgat acgagcagcc
     7141 ttaccagtac gctcacgcag gtagtacagt ttagctttac gaacagcacc acgacgtttg
     7201 acagaaatgc tgtcaactac cggagagtga gtctggaaga cacgctcaac gccttcgccg
     7261 ttggaaattt tacgaacagt gaatgcagag tgcagaccgc ggttacgaat agcgataacc
     7321 acgccctcga atgcctgcag acgttttttg gaaccttcaa caacccatac tttcacttcc
     7381 acggtatcac ccggacggaa ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc
     7441 ttaataatgt tgctcataat ttaatctctt atcctgggta aactgatctc tcgggggctt
     7501 acgccatccc atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca
     7561 accttgcttg ctcttcagtc agagccaggt tttccagaag ttcaggtctt ctaagccagg
     7621 tacggcccag cgactgtttc aaacgccagc gacgtatctc ggcatggttg cccgacagta
     7681 acactggcgg aacttccatc ccttctaaca cctcaggccg cgtatagtgc gggcaatcca
     7741 gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc atgtcccagt actcccggaa
     7801 taaaccggga aacggagtca atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt
     7861 aatcgccgat tgaccattct tcgtcaattt cggtttggat cacgcgctca tctatacctt
     7921 cgtagcgacc gcacaccaga atcaattttt gattcgttgc cagttcgctg acgcccgctt
     7981 gatcaagctt gcgtccctgt ggtgacagat aaatcacctt tgcgccttca cccgccgcgg
     8041 cttttgctgc atgaatggcg tcccgcaagg gttgcaccat cattaacatc cccggtccgc
     8101 cgccgtaagg acgatcgtcc acggtacggt gccggtcatg cgtgaagtcg cgaggactcc
     8161 aactctggat gctcagcagg ccatttttaa ctgcccggcc agttaccccg taatcggtaa
     8221 ttgcgcggaa catttcagga aacaggctaa ttatgccaat ccacatagcg ccgcctttta
     8281 ccgtttatcc ggtggtttaa aaaccaggat cccaatctac ttcgattgaa cgagtagtga
     8341 gatcgacttt cttgataacc tgcccatcga ggaacggtac gagacgttcc ttgataccaa
     8401 acgcatcttt caggtttgcc ttaatgacga gaacgtcatt agatccggtt tccatcatat
     8461 caacgacttt accgagatcg tagccttcag tggttactac ctggcagccc atcaggtctt
     8521 tccagtagta gtccccctct tcaagctgag gcagctgcga tgaatccacg acaatttcac
     8581 aattcgtcag caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt
     8641 cctgattgtg gtgcttccag ctttccagct ggacttgctg ccactgaccc gccttctgga
     8701 taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa aacactctga
     8761 gccacccacg aataccgtaa gacgaaccca tttttcccaa aacgatggga tcaacaggtg
     8821 cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac agattaagct gctttgttta
     8881 cttctttgat cagcgcagca acgcgatcag aaatagttgc gccctggcca acccagtgag
     8941 cgatgcgatc cagatccagg cgagtgcctt cttctttttc gctagcgatt gggttgaaga
     9001 aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg acaacaacct
     9061 ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg aatagttacc ataacatcct
     9121 cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga gtccggtgtc atattaaaag
     9181 cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc agttaataac tctgtcgcat
     9241 aaggtcattt agcgaccagg aaatcccggc ggcatcattc ccttcatgct tctcatcatc
     9301 ttcgccattc cgcctttctt cattttcttc atcatgcgct gcatgtcgtc gaactgcttc
     9361 agaaggcggt taacgtcctg tacctgcatc ccgcaaccgg cggcgatacg acgcttacgc
     9421 gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcattgagtt gatgatggct
     9481 tccatacgca ccagcacttt atcgtccatc tgcgacttga cgttatccgg gatctgcccc
     9541 atgcccggca gtttgcccat caagctggcc atgccgccca tatttttcat ctggcgcagt
     9601 tgctcaagaa agtcgttgag atcgaagccg tcgccttttt ttagcttgct ggctaatttc
     9661 tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag tacgtcgccc
     9721 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
     9781 tcgccaacac cgaggaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca
     9841 ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg taacgcttca
     9901 ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg
     9961 gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
    10021 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
    10081 ttcagcgccg cgttaacaat atcaaccggc ttctgaccaa catcagaagg gaagaaatca
    10141 acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg atagacgtca
    10201 gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaaattacc gagtttacca
    10261 acgctggttg ttttaccggc accttgcagg cctgccatca gcacgaccgc aggcggttgc
    10321 gcagccagat tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggacg
    10381 attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc aaccgctttc
    10441 tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc
    10501 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
    10561 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
    10621 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
    10681 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
    10741 cttctctttc ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg
    10801 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
    10861 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
    10921 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
    10981 ggttcattgg tcagtttggt gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
    11041 ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcgacc
    11101 ttcatgccca atgaatacat cacccatctg gaagcaacgc ctgggatgct gatgcacatt
    11161 ggcttatcgc tcttttccta tgccacgcta attatcgccg ccctgtacgc gctgcaactg
    11221 gcgtggattg attaccaact gaagaacaag aagctggcat ttaaccagga aatgccgcca
    11281 ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctgctaacg
    11341 ctcacgcttt gcactggcct gttctacatg cacaacctat ttagcatgga aaatatcgac
    11401 aaggctgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattac
    11461 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggcgc ggtaattctg
    11521 acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac ccagaaaagg
    11581 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
    11641 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
    11701 ttcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
    11761 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
    11821 ccgcgctcgg cactattgtc gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
    11881 ctggtgtgct gacttttgtc gtactggttt ttgctgaggt attgccgaaa accattgccg
    11941 cgctgtaccc ggaaaaagtc gcttatccga gtagttttct gctggctccg ctgcaaattt
    12001 tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg tatgctgatg cgcatgatgg
    12061 gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa agaagagttg cgcactatcg
    12121 tgcatgaatc gcgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg
    12181 atctggaaaa aatgaccgtt gatgacatca tggtaccgcg cagtgaaatt atcggtattg
    12241 atatcaacga tgactggaaa tctattctgc gccaactctc ccactcacct cacgggcgca
    12301 tcgtgcttta ccgtgattcg ctggacgacg ccatcagtat gctgcgagta cgtgaagcct
    12361 ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctgcgc gccgcggacg
    12421 agatctattt tgtgcctgaa ggtacgccgc tcagcacgca gttggtaaag tttcagcgca
    12481 acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcagggg ctggtgacgg
    12541 ttgaagatat tctggaagag attgtcggcg atttcactac gtcgatgtcg ccaacacttg
    12601 ccgaagaggt cacgccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc
    12661 gggaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg
    12721 gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggca
    12781 agtacgatat cgatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc
    12841 ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc
    12901 agggccgtga attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc
    12961 gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta
    13021 cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct gatgcacatt cgggtccagc
    13081 ggcacgttag tttcggcgat cacttcaaca ccaaacttac gcacaacatc cagcatcgac
    13141 ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat ccgggttagc tttatcagcc
    13201 acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc
    13261 agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gacgacgcag gttttccatt
    13321 tcggctttta cgcgcaaaat accgtcacgt tcacgggtct gggcttcagc cagctgagct
    13381 tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc ttctggctca
    13441 actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc
    13501 gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa
    13561 cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa
    13621 tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg
    13681 acacccacgg caccctactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac
    13741 aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaactgcaac tgaagaatgt
    13801 gaaaactggc acgctcgcgg agattgggca actggctgat ctcgcggtag tcgttggtgg
    13861 cgacggtaat atgctgggcg cggcgcgcac gctcgcccgt tacgatatta aagttattgg
    13921 aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg cccagcaaca
    13981 attagccgat gtgctggaag gtcactacat cagcgagaaa cgttttttgc tggaagcgca
    14041 agtctgtcag caagattgcc aaaaacgcat cagcaccgcg attaacgaag tggtacttca
    14101 tcctggcaaa gtggcgcata tgatagagtt cgaagtgtat atcgacgaga tctttgcgtt
    14161 ttctcagcga tctgatgggc taattatttc gacgccaaca ggctccaccg cctattccct
    14221 ctctgcgggc ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt
    14281 cccgcatacg ttgtcagcac gaccactggt cataaacagc agcagcacga tccgtctgcg
    14341 tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat
    14401 tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc
    14461 gaaagattac agttatttca atacattaag caccaagctc ggctggtcaa aaaaattatt
    14521 ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca
    14581 gtaatggttt ttcatacagg aaaacgacta tgttggcaca actgaccatc agcaactttg
    14641 ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga
    14701 ctggtgcggg taaatctatt gcaatagatg ccctcggtct ttgtctcggt ggtcgcgctg
    14761 aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt ttttctctga
    14821 aagatacgcc agcggccctg cgctggctgg aagaaaacca gcttgaagac gggcatgaat
    14881 gtttgcttcg tcgcgtgatc agcagtgatg gtcgctcccg tggtttcatc aacggtacag
    14941 ctgttcctct gtcacaactg cgcgaactgg gtcagttgct gattcagatc catggtcagc
    15001 acgctcatca attactcacc aaacctgagc accaaaaatt cctgcttgat ggctatgcca
    15061 atgaaacctc tctacttcag gaaatgaccg cacgttatca gttgtggcat caaagctgcc
    15121 gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg gaactgctgc
    15181 aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt gagcaaatcg
    15241 acgaagagta caaacgtctg gcgaacagcg gtcaattgct gaccaccagc cagaatgcat
    15301 tggcattaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac
    15361 aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg
    15421 aagaagctac catccagatt gctgaagcca gcgacgaact gcgccactac tgcgatcgtc
    15481 tggatctcga tcccaaccga ctgtttgaac ttgaacagcg catctcaaaa cagatttcgc
    15541 tggcacgtaa acatcacgtc agccctgagg cattaccaca gtattaccag tcgctactgg
    15601 aagaacagca gcaactggac gatcaggccg actcacaaga aacgctcgcg ctggcggtaa
    15661 cgaaacatca tcagcaggca ctggaaaccg cgcgcgcatt acaccaacaa cgccagcaat
    15721 atgcagaaga acttgcacag ctgatcaccg acagtatgca tgcgctctca atgccgcatg
    15781 ggcagtttac gattgatgtc aaatatgacg agcatcacct gggcgctgac ggtgccgatc
    15841 gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg
    15901 catccggtgg tgaattgtcc cgcatcgcac tggcaatcca ggtcatcacg gcgcgtaaaa
    15961 tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt ccaacagcgg
    16021 cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg tgtgttaccc
    16081 acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg
    16141 gtgcgatgac agaaacacat atgcaatctc tggataaaaa agcgcggtta caagagctgg
    16201 cgcgcctgct tggtggcagt gaagtcacac gtaacacact ggcgaatgcg aaagaactgc
    16261 ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac
    16321 cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga
    16381 gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact
    16441 gctgcagcag cagtactatt gatgttaacc gcaggctgtt ccactctgga gcgagtggtt
    16501 taccgtcctg acatcaacca ggggaactat ctgaccgcta acgacgtatc caaaatacgt
    16561 gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca
    16621 tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg
    16681 cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt tgaccaatat tgataacaaa
    16741 cctgcgctga gtggtaacta ataataaagt tgctctcaaa gacgttaaaa aaggtgctca
    16801 atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa
    16861 gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac
    16921 tatcgcttag ttttgccgga cggctgtaaa tgccgacttt gtttttagtt aaatcgatat
    16981 cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct
    17041 gcagcgtcac tcgctgcagg tattgcttct caggtagcgc ataagccacc tcaacggcaa
    17101 ttttacctag cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagcc
    17161 gccagctctt taaacacgcg accaaaggcg agttcaatca acttattggt aaactcaaag
    17221 tcgagatgaa actcgatacg acacgcctcc tggctcagcg gcgtaaactt ccatccacca
    17281 atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac
    17341 tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc
    17401 atctgcccag gagtggactc cagaatccga cttccggtac aacctggcaa aaactgagga
    17461 taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg
    17521 gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt
    17581 tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat
    17641 agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt
    17701 cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa
    17761 caagcgcgcc cgtcacgaat actttatcga agaagagttc gaagcgggac ttgccctgca
    17821 aggctgggaa gttaaatccc tgcgcgcagg aaaagccaat atcagcgaca gctacgtcct
    17881 tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ccgtggcctc
    17941 cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc agcgcgaact
    18001 ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta
    18061 ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaaa aacagcacga
    18121 taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcacgca tcatgaaaaa
    18181 cgcccaccgt taaacctgca ctccaattat tgaccagttc ctcaccgcgc ctccctctcc
    18241 ggcggcgcga atgaacatct tattggctat cacatccgac acaaatgttg ccatcccatt
    18301 gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata acgccattga
    18361 ggctggtcat ggcgctcata aatctggtat acttaccttt acacattggg gctgattctg
    18421 gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa
    18481 gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta
    18541 gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa
    18601 gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt
    18661 tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag catgtagtac
    18721 cgaggatgta ggaatttcgg acgcgggttc aactcccgcc agctccacca aaattctcca
    18781 tcggtgatta ccagagtcat ccgatgaagt cctaagagcc cgcacggcgc aagccctgcg
    18841 ggcttttttg tgctccgaat ttgtcccgcg aagtctgatg ccaactaatt aaatccgatc
    18901 cttttaggca ccttgttagg cacttcataa agctatattg tctttgaggt gcctaaaact
    18961 atggaaaccc ggcaatggca agacaaacca aacctctatc cgttaaggaa atcgaatctg
    19021 ctaaacccaa ggaagcggac tacgttctct atgatggcga tggccttgag ctactcatta
    19081 aatccagcgg gagtaaaatc tggcagtttc gctacattcg ccctgtcacc aagacacgag
    19141 caaagaaaag cataggccct tacccgtcag ttacccttgc cgatgccaga aactaccgtg
    19201 cagagtcccg ctcactccta gctaaacaga ttgagccgca ggaacatcag caagaacaat
    19261 tgcgtagttc gctggaagcc aaaaccaata ctttccagct cgtagctgaa cgttggtgga
    19321 atgtgaagaa agccagtgtg acagaggact atgcggaaga tatctggcgc tctcttgaaa
    19381 gagatgtctt tcctgcgatt ggcgacgtta gcgttacaga tattaaagct catacactgg
    19441 ttcaggccgt acaaccggtt caggccagag gagcactgga aacggttcgt cgcctgtgtc
    19501 aacgtattaa tgaggtcatg atctatgccc agaacacagg gctgattgat gctgttccca
    19561 gcgttaatat cggtaaagcc ttcgaaaagc ctcagaaaaa gaacatgccc agcattcgac
    19621 cggatcagct atctcaactg atgcagacaa tgcgaacagc cagcattagc ctttccacac
    19681 gctgcctgtt catgtggcaa cttcttacta ttacccgccc tgccgaagcg gctgaagctc
    19741 gctgggaaga agtagacata gaagcgcaag agtggaagat tcccgcagca cgcatgaaaa
    19801 tgaaccgcga ccatactgtt ccattgtcag atgaagcaat agctgtgctg gagatgatga
    19861 aaccgctaag cggcaaccga gaatttatct ttcctagccg tatcaagccc aaccagccta
    19921 tgaacagtca gactgttaac gcctcactaa aacgcgcagg ttttggtggt gtgcttgttt
    19981 cgcatggtct gagatcaatt gccagtacgg ctcttaatga acaaggcttt ccgcctgatg
    20041 ccattgaggc agcattggcc catgtggata agaatgaggt tcgtcgtgct tataaccgca
    20101 gcgattacct ggaacagcgt cgcccgatga tgcaatggtg ggcaaatttt gtgatggcag
    20161 cggatcgtgg aagcatgatt gaaggtggga taaaaggaat gaagctagtt gggtgactaa
    20221 atagctgcgc ggcatagtgt cccactttca gccagccctt gcaggttaag ccaccagaca
    20281 gtaaacggcg ggtgtcgcgt tatt
//
LOCUS       DAJTUN010000074        20245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTUN010000074 DAJTUN010000000
VERSION     DAJTUN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.074"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..105
                     /locus_tag="OO470_002468"
     CDS             <1..105
                     /locus_tag="OO470_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6251687.1"
                     /translation="GLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            115..639
                     /gene="ygaP"
                     /locus_tag="OO470_002469"
     CDS             115..639
                     /gene="ygaP"
                     /locus_tag="OO470_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6251688.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(686..1090)
                     /gene="stpA"
                     /locus_tag="OO470_002470"
     CDS             complement(686..1090)
                     /gene="stpA"
                     /locus_tag="OO470_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6251689.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1758..2207
                     /gene="alaE"
                     /locus_tag="OO470_002471"
     CDS             1758..2207
                     /gene="alaE"
                     /locus_tag="OO470_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6251690.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2244..2588)
                     /locus_tag="OO470_002472"
     CDS             complement(2244..2588)
                     /locus_tag="OO470_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6251691.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            2740..2970
                     /locus_tag="OO470_002473"
     CDS             2740..2970
                     /locus_tag="OO470_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251692.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3317..3562
                     /gene="nrdH"
                     /locus_tag="OO470_002474"
     CDS             3317..3562
                     /gene="nrdH"
                     /locus_tag="OO470_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6251693.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3559..3969
                     /gene="nrdI"
                     /locus_tag="OO470_002475"
     CDS             3559..3969
                     /gene="nrdI"
                     /locus_tag="OO470_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6251694.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            3942..6086
                     /gene="nrdE"
                     /locus_tag="OO470_002476"
     CDS             3942..6086
                     /gene="nrdE"
                     /locus_tag="OO470_002476"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6251695.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6096..7055
                     /gene="nrdF"
                     /locus_tag="OO470_002477"
     CDS             6096..7055
                     /gene="nrdF"
                     /locus_tag="OO470_002477"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6251696.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7410..8612
                     /gene="proV"
                     /locus_tag="OO470_002478"
     CDS             7410..8612
                     /gene="proV"
                     /locus_tag="OO470_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6251697.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8605..9669
                     /gene="proW"
                     /locus_tag="OO470_002479"
     CDS             8605..9669
                     /gene="proW"
                     /locus_tag="OO470_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6251698.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9727..10719
                     /gene="proX"
                     /locus_tag="OO470_002480"
     CDS             9727..10719
                     /gene="proX"
                     /locus_tag="OO470_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6251699.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            10911..12090
                     /locus_tag="OO470_002481"
                     /pseudo
     CDS             10911..12090
                     /locus_tag="OO470_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12214..12951
                     /gene="ygaZ"
                     /locus_tag="OO470_002482"
     CDS             12214..12951
                     /gene="ygaZ"
                     /locus_tag="OO470_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6251700.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            12941..13276
                     /gene="ygaH"
                     /locus_tag="OO470_002483"
     CDS             12941..13276
                     /gene="ygaH"
                     /locus_tag="OO470_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6251701.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13367..13897
                     /gene="emrR"
                     /locus_tag="OO470_002484"
     CDS             13367..13897
                     /gene="emrR"
                     /locus_tag="OO470_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6251702.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14024..15196
                     /gene="emrA"
                     /locus_tag="OO470_002485"
                     /pseudo
     CDS             14024..15196
                     /gene="emrA"
                     /locus_tag="OO470_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15213..16751
                     /gene="emrB"
                     /locus_tag="OO470_002486"
     CDS             15213..16751
                     /gene="emrB"
                     /locus_tag="OO470_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6251703.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(16815..17330)
                     /gene="luxS"
                     /locus_tag="OO470_002487"
     CDS             complement(16815..17330)
                     /gene="luxS"
                     /locus_tag="OO470_002487"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6251704.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17480..19036)
                     /gene="gshA"
                     /locus_tag="OO470_002488"
     CDS             complement(17480..19036)
                     /gene="gshA"
                     /locus_tag="OO470_002488"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6251705.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19109..19537)
                     /locus_tag="OO470_002489"
     CDS             complement(19109..19537)
                     /locus_tag="OO470_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6251706.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19534..20100)
                     /gene="yqaB"
                     /locus_tag="OO470_002490"
     CDS             complement(19534..20100)
                     /gene="yqaB"
                     /locus_tag="OO470_002490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6251707.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4881 a         5181 c         5060 g         5123 t
ORIGIN      
        1 gggcttatca acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg
       61 gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct
      121 ttgacaacca tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc
      181 gatattcgtg acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca
      241 ttatccgtgc tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc
      301 cactgccagg caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc
      361 cccgcagaaa tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca
      421 gtagcggtaa ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt
      481 ggtttaatat taatcggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta
      541 agtggctttg ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca
      601 aggttgttag ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc
      661 tagcagcgac atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg
      721 ccagcgcctg agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac
      781 cgttaacatc agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg
      841 gtgcagcagc agagctatta cccaataact cttccgggtt aattccgtca gctttcatca
      901 gctccagcca ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt
      961 cttcttcacg tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt
     1021 caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca
     1081 ttacggacat aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag
     1141 tgtaagcgat cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga
     1201 ttatttcagc tgttattttc tgaaactctc agaataattt caagattata tattagccat
     1261 tttgatgagt aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc
     1321 aagcctgaca aaaccgaagt gaataaaaat gcaatataac agaataaaag aaaacacccc
     1381 tcactggtgc tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc
     1441 cggtcacgac aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca
     1501 ttagtgaaat taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatatcgg
     1561 catgtaaaaa tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa
     1621 ctaactaaat aaaaattttt cactaattga ttagtcatag acagcgatat acgctatgca
     1681 aaaatgcaga tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt
     1741 tattaaggag cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata
     1801 cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg
     1861 gaatgagctt cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa
     1921 ttgcatggcc atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc
     1981 cgtcgggctg gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg
     2041 tgtatgtggc gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca
     2101 gttcaaacat cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt
     2161 gccgccgact gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc
     2221 gactggcatc gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt
     2281 tcaagcgcaa cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct
     2341 gctaaataga gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca
     2401 gccagtgttg aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt
     2461 cgcgcttcgc gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg
     2521 gttaccacat cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt
     2581 aaatacatat ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt
     2641 cacaacgctt tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt
     2701 ctacactgaa aattacatcg aattctaatg ggggatcata tgtataaccg accgaaccgc
     2761 aacgacgttg atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc
     2821 ctcgaatctg tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc
     2881 agtaaagctc aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc
     2941 cagcaagccg cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg
     3001 tgtagcgtgg gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcgtgcgc
     3061 aaatcgtagt gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg
     3121 gtttcttttt gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg
     3181 taatttcgat cactatttgc tatatattgt gtggttgaat cttttttcaa ctacatctag
     3241 tatctctgta tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta
     3301 aacggaaata cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca
     3361 cgccaccaaa cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg
     3421 cgttcctgaa gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat
     3481 tgctggcgat cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc
     3541 gccacacgcg gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg
     3601 cagcgtttta tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa
     3661 cggattcagg tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg
     3721 gctggcgcgg tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg
     3781 cttcgcggcg ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga
     3841 gatgtgattg cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg
     3901 caaagcgata tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag
     3961 aatgcctgac gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg
     4021 atagcgcagg tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga
     4081 cgcatgtgcg tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg
     4141 tcaacgaagg ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta
     4201 cgctgtttgc ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga
     4261 agttttacac cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg
     4321 ccgatcgagt cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc
     4381 aactgaccga tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact
     4441 gcggtaagca gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata
     4501 tggagtcgat tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg
     4561 tagcattttt gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc
     4621 aatcttctgg cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc
     4681 aactcggcgc gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc
     4741 tgcgttttct cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt
     4801 cgctgggcgt ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg
     4861 cgctgttttc gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca
     4921 ttagcgaaca ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg
     4981 cccgtgattt cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca
     5041 tgtatgaaga cacagtaaac cgtgctagcc ctatcgccgg gcgcataaat atgagtaatc
     5101 tctgctcaga aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg
     5161 cccgcacagg ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg
     5221 attcccccga tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag
     5281 atatgagtca tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca
     5341 tcggactggg gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt
     5401 cgccggaagc cctggatttc accaatctct atttctatac catcacctgg cacgcactgc
     5461 gtacctcgat gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac
     5521 gctatgccag tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg
     5581 cgaaagttgg cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg
     5641 cgcaactgcg cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc
     5701 cgccaaccgg gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg
     5761 cgaaagtaga gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta
     5821 tgactaacga aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca
     5881 tcgacaccta cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt
     5941 tccccgatac cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag
     6001 gtatcaaaac gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg
     6061 aaggctgcgt ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca
     6121 tcaactggaa caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca
     6181 atttctggct accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa
     6241 ctgtcgtaga acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc
     6301 tgcaaaatgt tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg
     6361 cggtattatc gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt
     6421 tctcgacgct gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg
     6481 caccgttgca gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga
     6541 aaaagaaaat cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc
     6601 cgatgtattt ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta
     6661 tccgcgatga agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa
     6721 agatatctct gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac
     6781 tctacgacaa cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg
     6841 atgtgaaagc gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac
     6901 cgttatttcc cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga
     6961 atgccgatga aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag
     7021 cggttgaaac agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact
     7081 acccgcagca gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt
     7141 gatctacact gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt
     7201 cgctatctat gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg
     7261 aaaattccgg cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct
     7321 cagattctca gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac
     7381 ataaacaaat aaaggaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt
     7441 ataaaatatt tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa
     7501 aagaacaaat tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca
     7561 ttgaagaagg cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg
     7621 tacgccttct caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg
     7681 atattgccaa aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg
     7741 tcttccagtc ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta
     7801 tggaattggc cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc
     7861 aggtcgggct ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc
     7921 aacgtgtggg attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag
     7981 ccttctcggc gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac
     8041 aggcgaaaca tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta
     8101 ttggcgaccg aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg
     8161 aaattctcaa taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta
     8221 gtcaggtgtt cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa
     8281 ctcctggctt cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg
     8341 gctacgttat cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa
     8401 ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg
     8461 atgcacaaac gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc
     8521 ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg
     8581 ctttagatcg tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca
     8641 gcggcggaca gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct
     8701 gacggcggtg gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat
     8761 attctcgatc cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc
     8821 gactgggttg ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat
     8881 atcctcaacg gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt
     8941 ttcgctctca tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg
     9001 ctgattgcca tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg
     9061 ttaactgccc tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga
     9121 agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg
     9181 tttgtttatc tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg
     9241 gtgacgatca tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag
     9301 gttccggcgg atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg
     9361 ttcaaagttc agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg
     9421 atgctggccc tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag
     9481 atggtacttc gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg
     9541 attgtgatcc tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc
     9601 agtcgcggca accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc
     9661 attaagtaac tctgcacttg cccggtggcg ccgggcatta tcaccctgcc aaaaaaagga
     9721 ataacaatgc gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa
     9781 acttttgctg ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc
     9841 actgaagaaa ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc
     9901 gtcaacaaac ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat
     9961 gcaaccttca ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt
    10021 ggcgataaga aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg
    10081 atcgataaga aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg
    10141 aagatcgcca aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac
    10201 cctggctggg gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac
    10261 accgtgactc ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac
    10321 aaagagggca aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg
    10381 aagccaggga aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa
    10441 aatgccgata ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat
    10501 atcgttgcca acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt
    10561 atgcagttgc cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc
    10621 tcagaaggcg atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc
    10681 gatggctggg tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg
    10741 cgttttcgcc tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat
    10801 catgcctaca aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac
    10861 atccgctatt attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac
    10921 ctaatcatga gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg
    10981 tagccagtaa ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt
    11041 ccgccagttc ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac
    11101 tgtttcttgt tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct
    11161 tactggcagc gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc
    11221 tcggtacggc attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag
    11281 cgacgctggc ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc
    11341 tgttggggat attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc
    11401 gcaccgtctt ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg
    11461 gtctgccaca aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt
    11521 tcagcatgtt catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct
    11581 ttgccaactt cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta
    11641 actacagcga tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg
    11701 ctcgtccggc gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt
    11761 ctgctgttac tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt
    11821 atcggcattc tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta
    11881 atttatcgaa tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac
    11941 tttattggcg gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg
    12001 gctggcgttt gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga
    12061 ggttttcatc gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca
    12121 cttataaggg tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat
    12181 ttataatatt aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct
    12241 cctggttcgg cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat
    12301 attccggtgg cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa
    12361 agcgtttttt tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg
    12421 ctggcagccg ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc
    12481 catgttttgt atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa
    12541 accgccctat gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg
    12601 gtacgcaata atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg
    12661 tcatcgtggg tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt
    12721 tatcccgccg ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg
    12781 ctcgcctctt tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt
    12841 gcaggcgtaa cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc
    12901 ctcactgcgt taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct
    12961 gcttgggtta ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg
    13021 tgtgggtaat gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg
    13081 catcgcctcg atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac
    13141 acgccgtttc gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac
    13201 acgcagcatt atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt
    13261 gatggcgatt atataaccca caagaatcat ttttctaaaa caatacattt actttatttg
    13321 tcactgtcgt tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt
    13381 tacgcccatt gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca
    13441 ggagatcctt ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa
    13501 taaaatgctg aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga
    13561 gtctcaggaa aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg
    13621 taccaacgcg acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa
    13681 aagcgataac gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt
    13741 gcgcgaggtt ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac
    13801 aacagaaaaa gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat
    13861 ggaacaagac ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc
    13921 agatttataa aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc
    13981 aggtcggctc agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga
    14041 gactcaaacc ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct
    14101 tctcaccttg ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact
    14161 gcgtcatttc gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc
    14221 tcaggtgtct ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg
    14281 cgatgttctg gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac
    14341 tgcactggct tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca
    14401 ggcgaatatt gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg
    14461 tgtgccgctg ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc
    14521 cgtcaccagt gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc
    14581 gatgattctg gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt
    14641 acgtaacgcc tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt
    14701 ttcccgtcgt gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt
    14761 cgttccagcc accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat
    14821 gcgcatcggt cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac
    14881 cggtaaagtg gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca
    14941 aaatgcgacc ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga
    15001 ccagaaacag ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa
    15061 taccactaac cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt
    15121 aagtaccgcg cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa
    15181 agctaacgct ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg
    15241 cgcaactggt cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact
    15301 ccaccattgc taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc
    15361 aaggaacgtg ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg
    15421 gctggctggc aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg
    15481 ctattgcgtc ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg
    15541 tgattcaggg gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata
    15601 actacccgcc agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg
    15661 cgccaatttg cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga
    15721 tattcttcat caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc
    15781 gcggacgtga aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg
    15841 ttattggtat cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt
    15901 catcacagga aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg
    15961 tctgggagct gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact
    16021 tcaccatcgg ctgcttgtgt atcagcctcg cgtatatgct ctacttcgac gctattgttc
    16081 tgctgccgca gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct
    16141 ctgcgccggt agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata
    16201 aactggatat gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact
    16261 ggcgtgccta tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta
    16321 tccaggggtt tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt
    16381 tgccaccgga acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg
    16441 ggtctatcgg cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg
    16501 cgcagttgac tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac
    16561 tggaagggct tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca
    16621 atcaggggct gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg
    16681 tactgctggg gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg
    16741 gtgcgcacta agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga
    16801 agatagttta ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct
    16861 tcgttgctgt tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc
    16921 tgcaacgagt gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc
    16981 tgatcctgca ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc
    17041 tcatctggcg taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata
    17101 tcgataatct ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc
    17161 tccagggtat ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga
    17221 tcgaacacgg tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca
    17281 ggcgcttcca tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct
    17341 ccggtaattt ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga
    17401 aagacgcgca tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga
    17461 gtggcctttt tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat
    17521 cagcggtttc catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct
    17581 cttcgcgcag aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg
    17641 ctttgcctgt tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag
    17701 tcagatcggg attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc
    17761 cgccgttaat gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac
    17821 ccacctgcag taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt
    17881 tgcgaccttc aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc
    17941 tcatttccgg tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct
    18001 gctgttcatc tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat
    18061 attcaatgcc gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt
    18121 ttggacgaat cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca
    18181 gcctcttacc gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg
    18241 cctgtttaag gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt
    18301 gcgatttatt ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat
    18361 acataccgca ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag
    18421 aacaaatcgc cggagatgca ccaaacagat aaggaatgac ccaaccgaaa cgatagtaat
    18481 tgcggataac gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat
    18541 caccgcactt cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa
    18601 tggtttgcat cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc
    18661 gtccggtgtt agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc
    18721 atggcatact taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca
    18781 gatcgcgcat aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca
    18841 gcaatgcttc cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt
    18901 caggatgacc tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca
    18961 gcccacgctg tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg
    19021 atacgtccgg gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt
    19081 aatggtgacc atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg
    19141 ctgctgcaac tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc
    19201 cccaggagat gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc
    19261 agcttaataa tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc
    19321 agcgcgatgt tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa
    19381 ggctattacc cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca
    19441 acattgccac cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac
    19501 tggcaaacaa cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc
    19561 catgcctgct gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg
    19621 cgtcggttgc acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg
    19681 gtgtttgacg tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc
    19741 cagcaatgcc tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc
    19801 atgccaactt ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact
    19861 tcttactgct tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag
    19921 ctcaataatt gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg
    19981 aatatcgtac tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg
    20041 ctccgtatcc aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat
    20101 ggtcttctcg caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc
    20161 tgatggcgaa ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata
    20221 tttttgaagg aaattttttg gagaa
//
LOCUS       DAJTUN010000075        19983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTUN010000075 DAJTUN010000000
VERSION     DAJTUN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.075"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..549)
                     /gene="aroD"
                     /locus_tag="OO470_002491"
     CDS             complement(<1..549)
                     /gene="aroD"
                     /locus_tag="OO470_002491"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6251708.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLT"
     gene            complement(580..1446)
                     /gene="ydiB"
                     /locus_tag="OO470_002492"
     CDS             complement(580..1446)
                     /gene="ydiB"
                     /locus_tag="OO470_002492"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6251709.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1458..2729)
                     /locus_tag="OO470_002493"
     CDS             complement(1458..2729)
                     /locus_tag="OO470_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251710.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(2912..4117)
                     /locus_tag="OO470_002494"
     CDS             complement(2912..4117)
                     /locus_tag="OO470_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251711.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4217..4573)
                     /locus_tag="OO470_002495"
     CDS             complement(4217..4573)
                     /locus_tag="OO470_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6251712.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4709..4816)
                     /gene="rprA"
                     /locus_tag="OO470_002496"
     ncRNA           complement(4709..4816)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO470_002496"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5002..6114)
                     /gene="ydiK"
                     /locus_tag="OO470_002497"
     CDS             complement(5002..6114)
                     /gene="ydiK"
                     /locus_tag="OO470_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6251713.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6503..9559
                     /locus_tag="OO470_002498"
     CDS             6503..9559
                     /locus_tag="OO470_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6251714.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9556..9966
                     /gene="menI"
                     /locus_tag="OO470_002499"
     CDS             9556..9966
                     /gene="menI"
                     /locus_tag="OO470_002499"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6251715.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10066..10254
                     /locus_tag="OO470_002500"
     CDS             10066..10254
                     /locus_tag="OO470_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6251716.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10802..11170
                     /gene="sufA"
                     /locus_tag="OO470_002501"
     CDS             10802..11170
                     /gene="sufA"
                     /locus_tag="OO470_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6251717.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11179..12666
                     /gene="sufB"
                     /locus_tag="OO470_002502"
     CDS             11179..12666
                     /gene="sufB"
                     /locus_tag="OO470_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6251718.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12676..13422
                     /gene="sufC"
                     /locus_tag="OO470_002503"
     CDS             12676..13422
                     /gene="sufC"
                     /locus_tag="OO470_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6251719.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13397..14668
                     /gene="sufD"
                     /locus_tag="OO470_002504"
     CDS             13397..14668
                     /gene="sufD"
                     /locus_tag="OO470_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6251720.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14665..15885
                     /gene="sufS"
                     /locus_tag="OO470_002505"
     CDS             14665..15885
                     /gene="sufS"
                     /locus_tag="OO470_002505"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6251721.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15898..16314
                     /gene="sufE"
                     /locus_tag="OO470_002506"
     CDS             15898..16314
                     /gene="sufE"
                     /locus_tag="OO470_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6251722.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16463..17467
                     /gene="ldtE"
                     /locus_tag="OO470_002507"
     CDS             16463..17467
                     /gene="ldtE"
                     /locus_tag="OO470_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6251723.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17530..17766)
                     /gene="lpp"
                     /locus_tag="OO470_002508"
     CDS             complement(17530..17766)
                     /gene="lpp"
                     /locus_tag="OO470_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6251724.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18077..19489)
                     /gene="pykF"
                     /locus_tag="OO470_002509"
     CDS             complement(18077..19489)
                     /gene="pykF"
                     /locus_tag="OO470_002509"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6251725.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5236 a         4791 c         5108 g         4848 t
ORIGIN      
        1 cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc ttaggaatat cggcgtcgaa
       61 ggactgcatt ttgcgcagac gggcaatgat ttcttcggct tccggcgttt tatggaagtc
      121 atggttggac atgacaactt tcacatcatg cgcgtgggcg taggcgacgg tttctttgac
      181 ctgatcatcg ccggtaaata actccagatc gatcatatca accaggccgc tgtcgatggc
      241 tgcacgattg agagcaatat aagcctcggt ggaaatcgcc tgctcgccgc cttctttggc
      301 actgcggaag gtaaacagca gcggtttttc tggcatggtt tcacggagaa tttttgccgc
      361 cgccatgaca gactccacat tggagaggtc ggcaaagtgg tccacacgcc attccagaat
      421 atcaaagtcc gcttcacgat aggcgagagc ttcggatttc acgcgggcga tatctttcgc
      481 catcagcgag acgatgattt taggtgcgcc cgcaccaatg acgagatctt ttacagttac
      541 ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt caggcaccga accccatgac
      601 ctgtttaaca tattccagag ggaaatcttt gccagtccat aacgtgaact gttcagcccc
      661 ttgccacaac aacatgccgt atccatcaat cgttttgcaa ccagcttgtt gtgcctgctg
      721 caataacttc gtcatatgcg ggttatacac gcattcagtg accagcagtc ccggatgtaa
      781 cagactgata tcattaacca atgattcatt ctcaaggggt ttcataccta cttttgtgcc
      841 attggttaaa atgtcggcgg aagccagggc ttcagcaaag gcttgctgat cggcgagatc
      901 ggtatccgtg acgacacaat cggtgttttc gttaacccgc tgcgcgaagg cgagggcttt
      961 atcgaagaac tcatcccgac ggttaaagag tttaatttct tttaaacctt caattgcccc
     1021 ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac agcaccatcg ttttgccttt
     1081 gatatcaaaa ccgctctctt taatggcgcg aatatggccc gtgccgtcgg tgttatagcc
     1141 acgcagatag ccatcatcat taacgatggt gttgatggca cccaccagtt tggctgctgg
     1201 cgttaattca tcaacatatt cacacaccag ttgtttgttc ggcatcgaca caccagttcc
     1261 gcgcattttg agggctttta atccttcaat tgctccggga aagctatcgt tatccacttc
     1321 gaaggccata taggtaaatg gcaatcccgc tttttctaag gctttattct gcatttcagg
     1381 cgataaactg tggcggatag gataagccat caatccaatc aattcgtatt ttgcggtaac
     1441 atccataatt aactccttta acctctatgc ttaattgtgt ttaaccgggt gctaaatttg
     1501 cgttcgccaa actgcacatc attctcagga ataataaaaa cgcggtaata gcggctaaaa
     1561 acaataattg cggtaatcag ggccagcagc gcgaaagtaa aatcgagaac aatgatatat
     1621 tgcaggccga tgttcgacag ataaccggta atcagcggaa taacaaagtt agccagtcca
     1681 cccatcatca tataaatact ggtgactttg gctttgcttt tggggaaaaa ctctgacatg
     1741 accgaaacac cgagctgtaa aatgccgcca gctgctgaga aaccgataac aaaggcgccg
     1801 gcattacaca ccagtggaga agggtacagg tagataatgg cagctgttat tgttgccagc
     1861 gcagagttaa atacattagc ccagatgggc cggaccattt ttttcagtag tgcggcaaaa
     1921 ataaagacac agaccaacga gcccatactg taataagaga tggtttttaa tgcctcagct
     1981 tctgacatac cagcaaaagc catcgcatat ttgggcatcc acaccacaat cacataaaag
     2041 gtcgagaatg cggctacacc gaacagtacc gatgaaacac cttccagcca gactaaaggt
     2101 ttgctgttca tttgcggtaa ttcattagct acgctggcgt ccaccaactg gctggggaat
     2161 ttgcttttca acagcatcag cgtgatcagt acaaacagaa tacccggaat aatcagccca
     2221 tagccgtacc agatattatt gagcaacata tagctcacca gcattgggta gaacatttgc
     2281 ccaaatgaca ccatcgcttt aaccagtatg accgccgaac cagaggcttt cggaaagcat
     2341 tccatgagcg cggggtagcc acccgtatcc agcgctgagt tagcgatacc tacgcacact
     2401 gccagaccgt aggcgagagt taaattcggg caagcgggaa taccaaagaa gaatagcaga
     2461 tacattatta ctgccattaa tatcaccgcc cgacgaccaa acttatcgga gatcacaccg
     2521 aagaataaaa tactgatcaa tcgccccaaa ccgataccgg aaattaaata ggcaatgccc
     2581 gcgttgtcag tggaaaactt ttctgccaga gatgtcatat tttgggcaag cgtaataaca
     2641 ctaatgccgt gcaggaaata gctgaagtaa atacaaagaa cagccaggat aaatggcgtg
     2701 ctgaaagata atttcgttac cgtacgtatt gttctccgac ggtttacata attactatac
     2761 tgaagatgat tttccctggg tacatggcat aaagtttatg tttccaggaa tattgcatgt
     2821 catagtttgt ttttgatcgc gattttcctt agttagcgca gaaagagtta tttatccgta
     2881 agatcttgca tgacctgaaa agaatattgc attacccacc cggagcgaca ttttccttta
     2941 gcgagtgatg ccgcgttttt ttgcggctgc gtaagccgat aaacagtgcc agtaaaaaac
     3001 cgatggcagt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     3061 tcaacggaat agtaaaggtc gcaatactgc ccgtactgta atagatccct gtagctttac
     3121 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     3181 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     3241 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     3301 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     3361 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     3421 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     3481 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     3541 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     3601 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     3661 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     3721 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     3781 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     3841 tggtctgcag gatgtcaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     3901 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     3961 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4021 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     4081 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     4141 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     4201 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     4261 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     4321 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     4381 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     4441 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     4501 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     4561 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     4621 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     4681 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggaaa
     4741 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     4801 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     4861 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctaccagctg aatggcggta
     4921 agcaggaaaa tatggggaag agcaagcaaa tatcaattag cggtgattaa caatcaggcg
     4981 gcagagccgc ctgatgagaa attacttatt cggtttctcg atttcgccaa gttcttcgag
     5041 aatttcttcc ggttggtccg tcggtggcgg gacttcttcc acccacgcgg caaacagacg
     5101 ccaggaaacg gctaacagaa ccggaccaat aaacagaccg atcatcccga aagcaatcaa
     5161 accaccaata acgccagaga ggatcaggat cagcggtaaa tcggcaccca tgcgaattaa
     5221 cattgggcgg atgacgttat ccagcgtgcc aaccacaccg ctccacacta acaataccgt
     5281 tccccaggtg gtatcgccgg tccagtagag ccagataatc gccggaatca gtaccggcaa
     5341 cgggccaagc tggacaaggc aggagaggat cattaacacc gttagcaacg ttgcataagg
     5401 tacgccggat acggcgaggc cgataccgcc aagcactgcc tgtactaacg ccgtcaccac
     5461 cacacccagc gccaccgcgc ggatagcctg tgccgccagc agcacggcgg catctccgcg
     5521 aacgcctgcc agacgggttg caaaatggcg aatgccttgt gccacctgtt caccgcgcca
     5581 gtacagcagg gcactgaaga gaagcatcag cgcacaatgc accataaagc gcccgatatg
     5641 cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata taagggcgga ctttcgccat
     5701 gatcgccgta ccccccatat ccagcaagtt gtgccagcct gcatacagct tcgcgccaat
     5761 caccggaatg gtattaagcc acgccagatc gggtaacgtc atgtcgccgc tggaaatggc
     5821 tttaattagc gggccgctgc cgtcgacgat actgttaacc agcaaagcaa tagggatgat
     5881 aaacaccatc actaataaca gcgtcatcac cagaacggcg agagagcggc ggccaaacat
     5941 gatcttttgc aaacgtaaca ataccggcca ggtggcgata accaccgtac cggcccatgc
     6001 aaagccgaga ataaagggtt gaacaatcca cagacatgcc acaatcatga tggctaaaaa
     6061 cagcaccgaa agcagaattt gtgcgacatc cctgggctga cgaacattta ccataactac
     6121 ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg aacacgcgaa actcacctgc
     6181 ataatgatga gcaatttcag cggtttttaa caggccgatt ctgcccttaa ttctgatggg
     6241 cgtgttagaa aaaaatgtgg tacaacaact caggcaacac gcaaacgatt tactcgtcgt
     6301 atttcaagtt gcatgtgctg cggctacgtt cgttcacccc agtcacttac tattgtaagc
     6361 tcctggagtt cactcgcttg ccgccttcct gcaactcgaa atccatagag taaataaaca
     6421 tcacctttca gtgcgctatt gcagccgcct gaaagaatag tgtatcgacc tacatcacag
     6481 accgcaggaa agggtcaata taatgattcc acagatttcc ctggcacccg gtgtcgttca
     6541 actggtgctt aattttttgc aagagctgga gcaacaaggt tttaccggcg atgcggcgac
     6601 aagttatgcc gatcgtctga caatgtcgac cgacaacagt atttaccaac ttctccccga
     6661 tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg atcgcccgtc ttgccgcgca
     6721 ggaacgctat tcatcgctga tctttacccc ccgcggcggc ggcaccggca ctaacggtca
     6781 ggcgctcaac caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat
     6841 taaccctgaa gagggctggg tgcgcgttga ggccggggtg ataaaagacc aactcaatca
     6901 gtacctgaaa ccgttcggct acttttttgc gccggaactt tcgaccagca accgggcaac
     6961 gctcggtggg atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac
     7021 gtcagatcac gtacttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca
     7081 acctttaccc gtcgagctgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat
     7141 ttataacacg gtttatcaac gttgccgtca gcaacgccag ttaatcatcg acaacttccc
     7201 caaacttaac cgctttctta ccggttacga tctgcgtcat gtctttaacg atgagatgac
     7261 cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga
     7321 agcgcggctg gatattacgc gcttgcctaa agtgcgccgt ctggtgaatg tcaaatatga
     7381 ctcttttgac tctgcgctgc gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt
     7441 agagacggtg gactcaaaag tgctgaatct ggcgcgggaa gatattgtct ggcattccgt
     7501 cagcgagttg attaccgatg tgcctgacaa agagatgctc ggactgaaca ttgtggaatt
     7561 tgctggtgat gatgaggcgc tgattgacga gcgagttaat gcactctgtg tgcggcttga
     7621 tgagctgatt gccagccaac aggcaggtgt gattggctgg caggtgtgcc gcgagctggc
     7681 gggggttgaa ggtatctatg cgatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa
     7741 aggtgccgct aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacatctggc
     7801 cgattatatt gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt
     7861 cggtcacgtc gacgcaggtg tcttgcacgt ccgtccggca ctggatatgt gcgatccgca
     7921 acaagagatt ttgatgaagc aaatctctga tgacgtggtg gcgctgactg cgaaatacgg
     7981 tggtttgttg tggggcgagc acggcaaagg ttttcgcgct gaatacagcc cggcgttttt
     8041 cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg gcatttgacc cgcataaccg
     8101 actcaaccca gggaagattt gcccgccaga aggtctcgat gcgccgatga tgaaagtgga
     8161 cgcggtgaag cgcggtacat tcgatcggca gatccccatt gcggtacgcc agcagtggcg
     8221 cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac tttgatgccc gtagtccgat
     8281 gtgtccgtcg atgaagatca cccagaaccg gattcattca ccgaaagggc gcgcaacgct
     8341 ggtgcgtgaa tggctgcgct tgttggcgga tcgcggcgtt gacccactca aactggaaca
     8401 agaactgcct gaatccggcg tcagtttgcg gacgttaatt gcccgcacgc gcaatagctg
     8461 gcatgcgaat aaaggcgaat atgacttctc acacgaagtc aaagaggcga tgtcgggctg
     8521 tctggcctgt aaagcttgtt cgacccagtg ccccatcaaa attgatgtgc cggagtttcg
     8581 ctctcgtttt ctgcagctct atcacacccg ttatttacgc ccgcttcgcg accacctcgt
     8641 tgctacggtc gagagctacg caccgctgat ggcacgcgcg ccgaaaacct ttaacttctt
     8701 cattaaccag ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc
     8761 actgctgtcg gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac
     8821 gctggaacag cttgaagctc tcaatgcaga gcagaaagcg cgcacggtgt tggtggtgca
     8881 ggaccccttt accagctatt acgatgcgca agtggtggcg gattttgtcc gtctggttga
     8941 aaaattaggt ttccagcctg tgttactgcc attttcgcca aatggcaaag cccagcatat
     9001 caaaggcttt cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg
     9061 tatggctaag cttgggatgc caatggtggg cgtcgatccg gcgctggtac tttgttatcg
     9121 cgatgaatat aaactggcgc tgggcgagga acgtggcgag tttaacgtct tacttgcgaa
     9181 tgaatggctg gcaagcgcac ttgagtcaca tccagtggct acagtcagcg gtgaatcatg
     9241 gtatttcttt ggtcactgta ccgaggttac cgctttgccg ggcgcgccag cacaatgggc
     9301 cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc agcgtgggtt gctgcggcat
     9361 ggcagggact tacggacatg aagcgaaaaa ccatgaaaat tcgctcggga tctatgagtt
     9421 atcctggcat caggctatgc agcgactgcc gcgtaaccgc tgtctggcga ccggatattc
     9481 ctgccgtagc caggtaaaac gggttgaagg cacgggggta cgccatcctg tgcaggcttt
     9541 actggagatt attaaatgat atggaaacgg aaaatcaccc tggaagcact gaatgctatg
     9601 ggtgaaggaa acatggtggg gttcctggat attcgctttg aacatattgg tgatgacacc
     9661 cttgaagcga caatgccagt agactcgcgg acaaagcagc ctttcgggtt gctgcatgga
     9721 ggagcatccg tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgaa
     9781 ggtgagcaaa aagtggttgg tctggaaatc aatgctaatc acgtccgctc ggcacgagaa
     9841 gggcgggtgc gcggcatatg taaaccgttg catctcggtt cgcgtcacca ggtctggcag
     9901 attgaaatct tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gactgccatt
     9961 ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt ttggtcatta tctgcacagt
    10021 aaacggtggt atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt
    10081 gatcccaccc agctggccat tgaattttta cgccgtgacc agagcaatct ttcacctgcc
    10141 caatatctca aacgtctgaa acaactagaa cttgaatttg ctgacctgct tactctctct
    10201 tctgctgaat taaaagaaga aatctacttt gcctggcgtc tgggcgtgca ttgatcctga
    10261 cttaatgccg caagttcgcc aaatcgacct ggattaacct ccgtccaggt tcgatgacct
    10321 tcttaatact gcttatctac cgcctgatat tgttcaaaaa taatactaat cgcagtttgt
    10381 gttaaaacgg cgggttagct ttatgagatt attcttatcg ccccttcaag agctaagcca
    10441 ctgagagtgc cggagataag cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag
    10501 acttaagggt tttcttattt ctgaacgctg cactttttta cctatctctt aaatagtcat
    10561 tttcaataca actttttctt tttcatcaca gccttaatgc tccttagata ataaccatta
    10621 tctaacaatg agatacctaa ttcttagcgt gcctgttaac ccacacatca ggggctatgc
    10681 ttattaaaag caggcaaaaa gaatgtttta cggtaaagcc cctgcgtttg ctgggttgaa
    10741 ctgataatca ttatcactaa catgctgtta tacgctgaaa gcgatgaagt gaggtaaata
    10801 gatggacatg cattcaggaa cctttaaccc acaggatttc gcctggcaag gcttaacgct
    10861 gacacccgca gcggcgatac acatccgcga gctggtggca aagcagcctg gtatggtcgg
    10921 cgtgcgctta ggtgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt
    10981 tagcgagccg gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc
    11041 gctgcaatcg atgccgttta ttgatggcac ggaagtcgat ttcgttcgtg aaggacttaa
    11101 tcagatattc aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt
    11161 tagggtatag gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg
    11221 accggcggcc cgctgaatta taaagaagga ttcttcaccc agttagccac cgatgagctg
    11281 gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag
    11341 tggatgctgg agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac
    11401 tggttgaaag cgcactacga caagctgaat tatcaggatt acagctacta ctcagcacca
    11461 tcgtgcggta attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc
    11521 gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg agcagttggg cgttcccgtg
    11581 cgggaaggca aagaggtggc ggtggatgcc attttcgact ctgtttcggt tgccaccact
    11641 tatcgcgaaa aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac
    11701 gatcacccgg aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc
    11761 tttgcagcac ttaatgcggc ggtagcctct gacggtacgt ttatttatgt gcctaaaggc
    11821 gtgcgctgcc cgatggaact ttccacctat tttcgcatta acgcggaaaa aaccgggcag
    11881 tttgagcgca caattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt
    11941 tccgctccgg tgcgtgacag ctatcagttg cacgcggcag tggtggaagt catcatccat
    12001 aaaaacgccg aggtgaaata ttccacggta caaaactggt tccctggcga taacaacacc
    12061 ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg
    12121 tcatggacgc aatcagaaac cgggtcagcg attacgtgga aatatcccag ctgcattttg
    12181 cgcggcgata actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa
    12241 gcggataccg gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg
    12301 aaagggatct ctgccggaca tagtcagaac agttatcgcg gcttagtgaa aatcatgccg
    12361 acggcaacca atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt
    12421 ggggcgcata ccttcccgta tgtcgaatgt cgcaataaca gcgcacaact ggagcacgag
    12481 gcaacgacat cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc
    12541 gaagaagacg ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcagaactg
    12601 ccgctggaat ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc
    12661 ggataaggaa taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct
    12721 atcctgcgtg gattaagcct cgacgttcgt cccggcgaag ttcacgccat tatggggcca
    12781 aacggttcgg gcaaaagtac cttatcggca acgcttgccg ggcgcgaaga ttatgaagtg
    12841 acgggcggca cggttgagtt caaaggcaaa gatttgcttg cactgtcgcc ggaagatcgt
    12901 gcaggcgaag gcatctttat ggccttccag tatccggtgg agattccagg cgtcagtaac
    12961 cagtttttcc tgcaaacggc acttaatgca gtgcgcagct atcgcggcca ggaaacgctc
    13021 gaccgctttg attttcagga tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa
    13081 gatttattaa cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat
    13141 attttgcaaa tggcggtgct ggaaccggag ttatgcattc ttgatgagtc ggactccggg
    13201 ctggatattg acgcattaaa agtggtcgcc gatggcgtga actcgctgcg tgatggcaag
    13261 cgctcattca tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac
    13321 gttcatgtgc tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa
    13381 ctggaggagc agggttatgg ctggcttacc gaacagcagt aatgcgctgc aacagtggca
    13441 tcacttgttt gaagctgaag ggacaaaacg ctcaccgcaa gcacagcagc atttacaaca
    13501 attgctgcgt accggactgc cgacacgtaa acatgaaaac tggaaatata cgccgctgga
    13561 agggctgacc aatagtcagt ttgtcagcat tgcgggagat atatccccac agcagcgtga
    13621 ttccttagcg ttaacgttag acgccgtgcg gctggtgttt gtcgatggac gttacgtgcc
    13681 cgcgctgagc gatgcaattg aaggcagcgg atatgaagtg agcattaacg acgaccgtca
    13741 ggggttaccc gacgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca
    13801 aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat
    13861 gcatatcacc cagggcgtgg caggtgaaga ggtgaacact gcccattacc ggcatcatct
    13921 ggatctggcg gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc
    13981 tcgtcatttt accggggcac ggttcactat caatgtcgca gcgaacgccc acttgcagca
    14041 tatcaagctg gcgtttgaaa acccggtcag tcaccacttt gcccataacg atttgttgct
    14101 ggctgacgat gccaccgcat ttagccatag tttcctgctg ggtggcgcag tgttacgaca
    14161 taacaccagt acgcaactca atggcgaaaa cagcacgctg cggatcaata gcctggcgat
    14221 gccggtgaaa aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg
    14281 taacagccga cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg
    14341 tttgatcaac gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaataatct
    14401 gctgatgggc aaactggcgg aagtggatac aaaaccgcag ctggaaatct atgcagatga
    14461 tgtgaaatgc agccacggcg cgacggtggg gcgtattgat gatgaacaga tgttctatct
    14521 gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg atcatttacg cctttgctgc
    14581 cgaactgacg gaagcactgc gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg
    14641 tcaacggctg ccaggaggtg caagatgact ttttccgtcg acaaagtgcg ggccgacttt
    14701 ccggtgcttt cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt
    14761 gcacagaaac cgagccaggt gattgacgcc gaggccgagt tttatcgtca tggctacgcg
    14821 gcggtgcatc gcggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg
    14881 cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc
    14941 ggcacgacgg aagggatcaa tctggtcgcc aatagctggg gcaacagcaa tgtgcgggcg
    15001 ggcgataaca tcatcatcag tcagatggag caccacgcta acattgtccc ctggcagatg
    15061 ctttgcgcac gcgttggcgc agagctgcgt gtgatccctc tcaaccccga cggtacgctg
    15121 caactggaga cgctgcctac gctgtttgat gagaaaactc gcctgctggc aattactcat
    15181 gtctccaacg tgcttggcac agaaaatcca ctggcggaaa tgatcacgct tgcgcaccag
    15241 catggcgcaa aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggtggatgtt
    15301 caggcgctgg attgcgactt ttacgtgttc tccgggcata aactgtatgg ccccaccgga
    15361 attggcattc tttatgtgaa agaagccttg ttgcaggaga tgccgccgtg ggaagggggc
    15421 ggttctatga tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccatgg
    15481 cggtttgaag ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag
    15541 tatgtttcgg cgctggggct taatagcata gccgagtatg aacagaatct gatgcattat
    15601 gcgctatcac agctggaatc tgtaccggat ctcactctct atggccaaca aaacaggctt
    15661 ggcgttattg cttttaatct cggtaaacac cacgcctatg atgttggcag ttttctcgat
    15721 aattacggca ttgctgtgcg taccggacat cactgcgcta tgccattaat ggcctattac
    15781 aacgtccctg cgatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggag
    15841 cgtctggtga ccggcctgca acgtattcac cgtctgctgg gataacaggg aggcactatg
    15901 gctttattgc cggataaaga aaaattgctg cgtaattttt tacgctgcgc caactgggaa
    15961 gagaaatatc tctacattat tgagctgggc cagcgtctgc cagaattacg cgccgaagac
    16021 ggaagcccac aaaatagcat tcagggctgc cagagtcagg tgtggattgt catgcgccag
    16081 aatgcgcagg gaattattga attgcagggc gacagcgatg cggcgattgt gaaagggctt
    16141 attgcggtcg tctttattct ctacgatcag atgacgccgc aggatattgt caatttcgat
    16201 gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc tcaccccatc tcgttcacaa
    16261 ggtctggaag cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa
    16321 gggacagctt tcatccggtt ggctcacatt gccggatggt caatgtttac actgacctgc
    16381 acaggagtgt tttggtttca ggtgaacata agcaccatcc gacgtttcag cgtgagtctc
    16441 cggcaaatac aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg
    16501 cgcttttagc gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggaag
    16561 ccgactggtt gggcaaaatc aaacgtatac ggtgcaagaa ggggataaaa accttcaggc
    16621 tattgcccga cgttttgata ctgcagcaat gttgatcctt gaagccaata acactatcgc
    16681 cccggtgcca aaacctggta cgacgataac tattccttca caactgttat tacctgatgc
    16741 accgcgtcag gggattatcg ttaaccttgc agagctgcgc ctttattatt atccgccggg
    16801 agaaaatatt gtgcaggttt atccaatagg tattggattg caggggctgg aaacgccggt
    16861 gatggaaacg cgtgttgggc agaaaatccc taacccaacc tggacgccta cggcaggcat
    16921 tcgtcagcgt tcgctggagc gtggcattaa attaccgcca gtcgttcctg ccggaccaaa
    16981 taacccgcta ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca
    17041 tggcaccagt gcgccggaca gcgttggttt gcgcgtcagt tcagggtgta ttcgcatgaa
    17101 tgcaccggat attaaagcct tgttctccag cgtgcggacg ggaacgccgg tgaaagtgat
    17161 caacgaaccg gtgaaatatt ccgtggaacc taacgggatg cgttatgttg aagtacatcg
    17221 accactatca gcagaagaac agcaaaacgt tcagacaatg ccatacacac tgccagcagg
    17281 ctttacgcaa tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta
    17341 tcgtcgggca gggtatccgg tttcggtgag cagtggagca actcccgcag ccagcaatgc
    17401 gccttcagta gagtcagcgc agaatggtga accagagcaa gggaatatgt tacgcgcgac
    17461 gcagtagtgg taaatagcag acaaaaaaat ggcgcacaat gtgcgccatt tttcacttca
    17521 caggtactat tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc
    17581 tgcgtcatct ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg
    17641 gtcaacttta gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct
    17701 ggagcaacct gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc
    17761 tttcattatt aataccctct agattgagtt aatctccatg tagcgttaca agtattacac
    17821 aaagtttttt atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc
    17881 tcaaagaagc atcgaacgct gggttttaga taaaaaaatt gagagaaaac aggatgcttc
    17941 catcggattc atcttaaata aagtgagatt atatagtgaa atccgatttg attttttatt
    18001 gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat atacaaatta
    18061 attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg ccgctcggta
    18121 ccagtgcacc agaaaccata actacgacgt cacctttgtg tgccagaccg ctctgcagag
    18181 ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta accagctgcg
    18241 gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg gtcagtgcca
    18301 ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg ccctgggtag
    18361 caaccacgat cagcggagca tccagttttt cagcagtttc aacggcacca cggcataccg
    18421 cttcggtgat gcgcagttta cggttgtcat tgttgaactc gagacggctg ttcatcacgc
    18481 ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg tatttacctt
    18541 ttgcggattc accagacagc atcactgcgt cagtaccgtc gaggatggcg tttgcaacgt
    18601 caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc atctgggtcg
    18661 cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc tgggcgaaga
    18721 taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg atgccgtcag
    18781 aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt ttggagatga
    18841 tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata acgtcagaac
    18901 gcttacgaat aaaggaagca gcaacaaagt ctacgccttt ttcgcaacca aagatcaggt
    18961 cctgtttgtc tttttcagcc agtgctggca gagcaatgga aacgccaggc aggttcacac
    19021 ctttgttttc gcccaggtca ccgttgttca gcactttaca gataactttg ttaccttcaa
    19081 tggcggtaac ttccataccg atcagaccat cgtcaaccag tacggtgttg ccaacagaca
    19141 ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg ataacagatt
    19201 tatcagtggt gaaagtaaag gtctgaccag ctttcagaga aacatcgtta ccgccttcca
    19261 gtttcatggt gcggatttcc ggacctttgg tatcaagcag gatagcggcg gttttaccag
    19321 ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca tagtcaccat
    19381 gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct aacatctctt
    19441 cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg acagtcttag
    19501 tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg gaaagcgaat
    19561 ccggtattac gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga aaaagcgtgc
    19621 agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgtc tgtttttgag tcgttgagtt
    19681 ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat acgctgaaat
    19741 taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt agacaataaa
    19801 ttgttatcaa agatttaacg ttccagggaa aaggttacgc aaaaacttcg ctgtttgcgc
    19861 aacgtctggt gccgaagttg tcagtgtgag gataagtgaa agttcatccg gtgtataaat
    19921 atatcatcgc tcaattgcta cagttaaaat aatttatcgc gttatttgtc tgattttgtt
    19981 tga
//
LOCUS       DAJTUN010000076        19741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTUN010000076 DAJTUN010000000
VERSION     DAJTUN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.076"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..780)
                     /gene="manZ"
                     /locus_tag="OO470_002510"
     CDS             complement(<1..780)
                     /gene="manZ"
                     /locus_tag="OO470_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6251726.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVN"
     gene            complement(793..1593)
                     /gene="manY"
                     /locus_tag="OO470_002511"
     CDS             complement(793..1593)
                     /gene="manY"
                     /locus_tag="OO470_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6251727.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1656..2627)
                     /gene="manX"
                     /locus_tag="OO470_002512"
     CDS             complement(1656..2627)
                     /gene="manX"
                     /locus_tag="OO470_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6251728.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3090..4640
                     /gene="yoaE"
                     /locus_tag="OO470_002513"
     CDS             3090..4640
                     /gene="yoaE"
                     /locus_tag="OO470_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6251729.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4644..6242)
                     /locus_tag="OO470_002514"
     CDS             complement(4644..6242)
                     /locus_tag="OO470_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6251730.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6373..7737)
                     /gene="sdaA"
                     /locus_tag="OO470_002515"
     CDS             complement(6373..7737)
                     /gene="sdaA"
                     /locus_tag="OO470_002515"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6251731.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(7921..8499)
                     /locus_tag="OO470_002516"
     CDS             complement(7921..8499)
                     /locus_tag="OO470_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6251732.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(8503..9864)
                     /gene="pabB"
                     /locus_tag="OO470_002517"
     CDS             complement(8503..9864)
                     /gene="pabB"
                     /locus_tag="OO470_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6251733.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            9938..10117
                     /locus_tag="OO470_002518"
     CDS             9938..10117
                     /locus_tag="OO470_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6251734.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10237..10596)
                     /locus_tag="OO470_002519"
     CDS             complement(10237..10596)
                     /locus_tag="OO470_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6251735.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(10958..11302)
                     /locus_tag="OO470_002520"
     CDS             complement(10958..11302)
                     /locus_tag="OO470_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6251736.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11434..13344
                     /locus_tag="OO470_002521"
     CDS             11434..13344
                     /locus_tag="OO470_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6251737.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13402..14097
                     /gene="tsaB"
                     /locus_tag="OO470_002522"
     CDS             13402..14097
                     /gene="tsaB"
                     /locus_tag="OO470_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6251738.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14137..14718
                     /gene="yeaY"
                     /locus_tag="OO470_002523"
     CDS             14137..14718
                     /gene="yeaY"
                     /locus_tag="OO470_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6251739.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            14923..16608
                     /gene="fadD"
                     /locus_tag="OO470_002524"
     CDS             14923..16608
                     /gene="fadD"
                     /locus_tag="OO470_002524"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6251740.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16690..17805
                     /gene="rnd"
                     /locus_tag="OO470_002525"
     CDS             16690..17805
                     /gene="rnd"
                     /locus_tag="OO470_002525"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6251741.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17859..18824)
                     /gene="yeaX"
                     /locus_tag="OO470_002526"
     CDS             complement(17859..18824)
                     /gene="yeaX"
                     /locus_tag="OO470_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6251742.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18880..19497)
                     /gene="yeaW"
                     /locus_tag="OO470_002527"
                     /pseudo
     CDS             complement(18880..19497)
                     /gene="yeaW"
                     /locus_tag="OO470_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         4832 a         4948 c         5132 g         4829 t
ORIGIN      
        1 gttaactttt ttgcgcagca gccacataca agcaaatgtc agcagcagtg gtacaaggcc
       61 tggcattaac tggtcaagaa tagtttgcac ggtggtgaca cgggtctgtc catcctgccc
      121 ggtgatggtc gaaaccacca gcgggatgtt gacatgtgtc cacttgttaa ccaatgcccc
      181 catgacaaac aggccgagga tagacgcccc ttccgtcagt ttttgcagga agccaccacc
      241 catatcttta acgatatcga tacctttgga gtaaccatac gctacgccgt agtaacgggt
      301 tgccagacgc accaggttaa acaggatgaa gaacagcagc ggacctaaca ggctgccgct
      361 catcgcgata ccggcaccca gtgctgcaaa taccggacgt acggttcccc agaagatcgg
      421 gtcgcctaca ccagccagcg gccccatcaa accgactttg ataccgttga tagcaccgtc
      481 atcgatctct gcgccattag cacgctgttc ttccagcgcc agggttacgc cgagaatcgg
      541 tgcagccacg aacggctggg tgttaaagaa ctccaggtga cggcgaatag cttgtttacg
      601 agcttcgttg ttctcagggt agaggcgacg aattgccggt accatagaga agcagaaacc
      661 cagtgcctgc atacgttcga agttccatga accctggaag aggttagaac gcaggaatac
      721 gccacgaata tcactttgag tgagtttttt ctcggtggta gtttgagttg tatcaaccat
      781 ttcgctcacc tgttagtcca gttcgttatc gagatcgttg ttaccagctg cctgagcagg
      841 cgcaccggct acgcggttgt atttcgggct aagttggatg tagagcactg ccataacagt
      901 accaatcaca cccagagcaa ccaggttaaa gttggtgaat gctgcggtta cgaagccgag
      961 gtagaagaac ggcatcaggt agccagcacg catcatgttg ataaccatcg cataaccaac
     1021 caccacgatc atgcccccgg cgatattcag accattggtc accacttccg gaatcgcatt
     1081 cagcatgttc tgtacttcgc tggtaccaac agacagcgca acgatgacgg ccggaatagc
     1141 cacacgcatt gcttgcagga acagagaaga aacgtggatc caggaaatcg ctgtcaggtt
     1201 gccgttatca gcagccttat ccgcagcgtg ctggaaagca acggtaatag tacgaacgat
     1261 gatggtcagt acctggcccg cagcggccag agggattgcc agtgcgatac ctgcaccaat
     1321 gctctgatga cctgcgataa ccagaatggt agaaatgata gaagccagag cggcgtcagg
     1381 cgcaactgca gcaccgatgt tcatccagcc cagcgcgatc atttccagcg taccaccgat
     1441 aataataccg gttttcatat ccccaagaac gatacccacc agggtacacg cgattagcgg
     1501 acggtgaaac tgaaattcat cgaggattga tcccatacct gcgatacagg ctacgataaa
     1561 taccagcaca atttgaagag tggtaatctc cattgtactt ctcctgttta cgtaagactt
     1621 aagtgtgaaa actgaatgat aatcaacaca atacgttact tatcgatttt gctgatcaga
     1681 tccatcattt tcagtttcgg atcggtggaa accttacgga cttccagctc aataccgcgt
     1741 gcattcagtt ttttgaacgc ctcgatatct ttttcatcaa ccgaaaccgc gttattcacc
     1801 tgggttttac cctgacggaa tgccatacca ccgacgttaa cagaggtgat tttcacgccg
     1861 ccttcaacga gacgctctac atctgttggg ttggtaaata acagcattac gcgttcgcca
     1921 gcatatttcg ggttgttgta gacgcgaatc attttggcaa catcaactac gtgtgctgtt
     1981 acgcccggag gtgcaacctg ggtgagcagt gtcttacgaa cggtatccgc agccacttca
     2041 tcactaacaa caataatacg ggagacattg gtttctttgg tccagcgggt ggcgacctga
     2101 ccgtgaatca gacggtcgtc gatacgcgca aggccaataa ccatgtagtc gtttggcacc
     2161 attggttttg ccggagttgg agccgctttt ggtgctgcgg cagcgggtgc tggcgcggct
     2221 ttttcaaccg gtttggcttt cagtgctttc acgccttcac ggcctgtttc tactgccagc
     2281 gcaaccagtt catcaaagct cgggtcatca tcacgggcca ttaacgtttc cacgagcatt
     2341 ggaatgttaa cgcctgcaat gacttcataa tgctctttgt cgacgacaat gcggctggca
     2401 gcattgaacg ggctgcctcc ccatgtatca acgagaaaca gcacgccttt agtggtgtcg
     2461 agttttgcca actgagcgtt gtatttttca atcagcgttt cggcattttc acctggaacg
     2521 aaatcgatcc agccgacgtt ttcctgctcg cctaacagca tttctgccgt tttaagcaac
     2581 tgctctgcag cccaaccatg tgtgcctata acaatagcaa tggtcacttg ctacctcctt
     2641 tattatcgtt aacacctcaa cgtgccagat gtatttttga atcgtcgtcc acaatcgaat
     2701 cgattcagat aagggctaca accaaaaacc cctgcgtttc gcgatttatt ttagatatcg
     2761 aaaaaattat tttatgtgat gaagatccgt aatttaactt tcgattatca gaaatttcga
     2821 aaggtaaaat atccttgtca cattcgtttg caaaggaagg taaatctttg ccaaatcaga
     2881 ggcgtctctg atatgtttaa ctcccgttta acaaccatgg agtatagggc agtagcccgc
     2941 agtatggatc gtcaccgacg tcatttcagc atcaggcctt ttaacgcctg cctttctggc
     3001 actctatgcc gcacctttcg tttgcatttt gtcgttacgc ctgcattatt tctggcgtcg
     3061 aatagctatt ccttaagcag gagcttgtca tggaattctt aatggacccc tcaatttggg
     3121 cggggctact cacgcttgtt gttctcgaaa ttgtgctggg tatcgataac ctggtcttca
     3181 tcgccattct tgctgacaaa ctgccgccaa aacaacgcga taaagcgcgt ttgctggggt
     3241 tatcactggc gctgattatg cgtctggggc tgctgtcgct gatttcatgg atggtcacgc
     3301 tgaccaaacc gctatttacc gtcatggatt tctccttctc cggacgcgat ctgattatgt
     3361 tgttcggggg gatattcttg ctgttcaaag caacaaccga actgcatgaa cggctggaaa
     3421 accgcgatca tgattccggc cacggtaaag gctacgccag tttctgggtg gtcgtcacac
     3481 agattgtcat ccttgacgcc gtcttctcgt tggatgcggt aattactgca gtagggatgg
     3541 ttaaccatct gccggtgatg atggcggcgg tagtgattgc gatggcggtt atgttgctgg
     3601 catcgaaacc gctgacgcga ttcgttaacc agcaccccac ggtggtggtg ctctgtctga
     3661 gcttcctgtt aatgattggt ctgagtctgg tggcagaagg tttcggtttc cacattccga
     3721 aaggttacct gtatgccgcg attggcttct cgatcattat tgaagtgttt aaccagattg
     3781 cgcgtcgcaa ctttattcgt caccagtcga ctttgccgct gcgagcgcgt actgccgatg
     3841 ccatcctgcg tttgatgggc gggaaacgtc aggccaatgt tcagcacgat gccgataacc
     3901 cgatgccgat cccggaaggt gcatttgccg aagaagaacg gtacatgatt aacggcgtac
     3961 tgacgctggc gtcgcgttct ctgcgcggga tcatgacgcc gcgcggtgaa ataagctggg
     4021 ttgacgctaa tctcggggtc gatgaaatcc gcgagcaact gctctcttca ccgcacagtc
     4081 tgttcccggt atgtcgcggt gaactggatg aaatcatcgg tatcgtacgt gctaaagaac
     4141 tgctggtggc gctggaagag ggcgttgatg tggcggcgat tgcttcggcg tctccggcga
     4201 ttatcgtccc ggaaaccctc gatccgatca acctgctggg cgtgctgcgt cgcgctcgcg
     4261 ggagctttgt tatcgtgacc aacgagtttg gtgtggtaca aggcttggtc acgccgctgg
     4321 atgtgctgga agccattgcg ggtgaattcc cggacgctga cgaaacgccg gaaatcatta
     4381 ccgatggtaa cggctggctg gtaaaaggcg gtacagattt gcatgccttg cagcaggcgc
     4441 ttgatgttga gcaccttgcc gatgacgatg atatcgcgac ggtcgcgggc ctcgtgatct
     4501 cggcaaatgg tcacattccc cgtgtgggcg atgtgattga tgtagggcca ctgcatatca
     4561 ccatccttga agccaatgat tatcgtgttg atctggttcg cattgttaaa gagcaaccgg
     4621 cgcacgatga agatgagtaa gcgttaacgt aacgacataa tgggcgtgat atgtccatta
     4681 tgccgggcgg gcggcggttg gctgcccgcc agccatttgg gaaaatcacg tagcggcatc
     4741 ggctgtgcgt acaaataccc ttgcaaaata tgcacgccat gacggcgcaa atacttcgcc
     4801 tgttcttgcg tttccacgcc ctccgccacc agttcaatat tcagtctttg ccccagcgcg
     4861 atgatgatat cggtcaccgt cgagttaacc gcgtcagaac ctatagctgc ggtaaatgac
     4921 ttatcaattt tcagcacgtc aggacgtaat gtttcaagcc aggaaaacga gctgttgccg
     4981 gtgccgaagt catcaatcgc cagtttgacg tttttacgat gcagctcgcg ggcaatccga
     5041 taatcaacat ccagtaaggc atcgcgttcg gtgatttcga ggatcagttg ctgaatcggg
     5101 tgagcgctaa accagtactg attgagatct tttatcagca caccacggcg aaaatggctg
     5161 ggtgcgacgt taatgccaac atgaaactga ctactcatcg ggaaaacatg gtgctgacga
     5221 atggtttctg ccatcacata gtgggtcagt ggcacaatta aatgatgttc ttccgcgata
     5281 ggaataaaca catccggtga aatccagccc tgacgcggat tattccagcg cagcaaaatc
     5341 tctacaccaa tacattgctg gctgcgcgca ttaagcaaag gctgacagaa caattcgaac
     5401 tcatgttgcg ccaggcccag attaatttcg cgggaaaagc tcatccggta agcggtcgcc
     5461 agccaggcga tatagcccac cagtaaactt agcagcaccg ccagcggtaa ttgtgttgga
     5521 agatagtgcc atgccagcgc cgtagccccc ggaccattta cgttaatggt aaaaggaaag
     5581 ctttgcgaag aaacctgata gttttcattg tcttcaggtt gcggaaggga atctaccagc
     5641 ccattaccat agagcaaatg ccgtttgtcc accgtcaggc tggcgctact gatttgcggc
     5701 agttgtggct caaggagcat tgccgtcagt aagtcgatgt taatcatctc tatgacccca
     5761 gcattgctac tgcccgctgc aggcgtccat tgaatcaaaa ccggactgcc tttaatcagg
     5821 gcacggtcga tcgtcaggcg taaaagtggt tgcggtgcag gaagttcagc cagaatgtcc
     5881 acgacgggca cattgcgata accaaaaatg ctggagcaat aaagtgtgcc gtcttgcacc
     5941 aggccaatgg atcgcacagt ttggagtttt gccgcctgtt tacgtaatgg caaatgggcg
     6001 acagagcagg gcagaccaat cagcggaagc aagactttgc tacctgcctg taggggaagc
     6061 agtactttat ctaattcctc tacagcgtga ttggcgaatt gtactacccg ttgttgattt
     6121 aagttacgct gtgaaataaa tcgcacgctc agggtcgaag cgagcgtaac gagtgcgcaa
     6181 atcgtacaaa caatcattcg attacggcga taggttttaa tgatccgttg tgctttttgc
     6241 atgcgggcca ccctgtaagc gtatggcgac aaacgcctga cgcaacagtg gaagtgtagt
     6301 ggggaatatt acagcagacg agaaagcggg tataaattcg cccatccgtt gcagatgggc
     6361 gagtaagaag tattagtcac actggacttt gattgccaga ccaccgcgtg aggtttcgcg
     6421 gtatttggcg ttcatgtcct taccggtttc gtacatcgtt tcgatgacct tatccagcga
     6481 gacgcgcggt gcactggtgc ggcgcagagc catccgtgcg gcgttaatcg ccttcacaga
     6541 ggcaatggca ttacgctcaa tgcacggcac ctgaacctgc cctgcaaccg ggtcgcaggt
     6601 taaaccaagg ttgtgttcca tgccaatttc cgccgccacg caaacctgtt ccgggctacc
     6661 gcccagcagt tctgcaagac ccgcagcagc cattgaacag gcaacaccca cttcgccctg
     6721 gcaaccaact tccgcaccgg aaatagaggc gttcatttta tacaatgcac caatcgcgcc
     6781 cgctgccata aagtaacggg tatagatgtc tgggctgacc gattcaataa agtggtcata
     6841 gtaagccagc actgccggaa cgataccgca ggcaccgttg gttggcgcag ttaccacacg
     6901 accaccggcg gcgttttctt cgttaactgc cagcgcaaac atgtttaccc agtcaatgac
     6961 attcatcgga tcgttagaca gtttatcgct ggaaactaac atccggcgca gggcagacgc
     7021 acgacgtggc acgcgcagcg ggcctggcag tacaccttcg gtgttcatcc cgcgatcgat
     7081 acatgcctgc atggtttgcc agacatgcgc gaaatactcg tcgatctctt tcttgctgtg
     7141 cagcgccagt tcgttctgca tagcgagacc agacagcgaa tagccggttt cattacagta
     7201 cgcgagcagt tcggtggcag atttgaacgg atacggcacg ctcacttcgt tggcagcatc
     7261 ctgaccaaag tgttcttcat cgacgataaa accgccgccg atggaataat aagttttgct
     7321 gtagacgact tcatcgccgt tataggcgtg gatttgcata ccgttttcat gcagcggcag
     7381 gttgccgtta tgaaaacgca tcccgttgtc gcgcgggaaa tccacttcat gccgtccctg
     7441 tgccagcagc agacgttcgc gctcttttac gtcgcgaata aaaccgggaa tactgtcgat
     7501 atccacggtg gcaggttcgt tacctgcaag acccataata atggcgatat cggtgtggtg
     7561 gcctttaccc gtcagcgaca gtgaaccata aacgtccacg gcaacgcgag taacgctatc
     7621 cagtaagcct ttttcgacca gatcatcgac gaactgttta cccgccttca taggccctac
     7681 ggtatgggaa gatgagggac caatccccac cttaaacatg tcgaatagac taatcacgat
     7741 aatactcctg acaaggtgac tggacttcca gtaacgaact aataacagcg catagtgtaa
     7801 gagggaacgc ggcgactggc ttaactattc acatgaatta aactaatcaa aaaccgcgat
     7861 ttaactaata ttgcgatgat tgtctcaagt agtgttttgt agatgtaaag ataagtatag
     7921 tcaggggttc acaccaattt gcagcgccag ctcacgaatt atgcctgcgg tcattcccca
     7981 tacaaaatac tgttcgtacc aggacagcca tacccgatgt gaatcgccac ggcggtagat
     8041 atctaaaggg tgataacgac ccagatgtaa tgcctgggcg agcggcattt caaacaccgc
     8101 cgagacttca tcttcactgg cgcgatacgg cagatcgggc gggataatgc cgaccactgg
     8161 ggttacttga tagccagtta cgctatcgac gggcggcagc acgccgataa cttcaacggc
     8221 ggaaggcggt atagcgacct cttcttcagc ttcgcgcagc gcggcggcga taactgatgc
     8281 gtccgtgtca tcgactgcac ctccagggaa tgccacttgt ccagcgtgtt tacgcagatg
     8341 aattgaacgc tgagtcagca acaaccccgg ttgcggtcga cggacgatgg ggattaacac
     8401 ggcagcctga cgatgattta ggggttcccg gttaatttgc gggcgcaaaa gttgaaagcg
     8461 agataaaaaa tcatcaagcg tcaggctacg gtattccacg tcttacttct ccagttggcg
     8521 taatatctta ttaactttat caaaagtttc ctgatattcc gcttcttcct ggctatcggc
     8581 gacaattcca ccgcccgcag agcagtatat ttgtccgtta atggcagtca gcgtgcggat
     8641 agtaatgctg gtatccatgt tgccgcaaaa gctcaaatag ccaatgctgc cgcaccaggc
     8701 attacgtcgc tgcggttcca gttcgtcgat aatttccata gcccgtactt tcggagcccc
     8761 ggttattgag ccaccaggaa aagctgcgcg cagcagatcg ctggcgtgta actgttctgg
     8821 tagtcgcgcc gttatagtgc tgaccagatg atgcacggca gggaagggtt ccaccacgaa
     8881 gagctctggt acttttaccg aaccggctac ggcaacacga ccgatatcat tacgcattaa
     8941 atcgacaatc atcagatttt cggcacgatc tttcgctgag ttcgccagtt tttctgcttg
     9001 tttgctatct tcctgaggat cgggcaggcg tggtagcgtg cctttaatcg ggcgggtctg
     9061 gatttcacta ttatcacaaa gaataaaccg ctctggcgaa aggcttaaaa ttgcaccctg
     9121 ttcaagacgt aaaaaagcgc taaatggcgc gcggttggcc tgattaagct gaaggaatgc
     9181 ctgccattca tcgccagaat aggtcgcatg aaaacgctgg gcgagattca cctgatagca
     9241 atcaccgctg tgcagatatt cctgtacctg gcgaaatttt tcgccgtact gctcgtgggt
     9301 catattggat tgccagtcgg aagtgagcgt gaaatcttcc tgcggcgaga attgctggct
     9361 ttccagccag gcccgacggg cattgacatc attatgactc agcaaagaaa ctgtatgacg
     9421 ctggtggtcg acaatgagcg cccaatcgta gatacccact gccatatccg gcagaacgat
     9481 atcttgttcc gcaatttctg gcagtgactc aaaacggcgg cccagatcgt agccaaacaa
     9541 ccccagtgcg ccgccctgaa atggcaaatc ttcgttatgc gttgggcgaa tgtctgcgcg
     9601 atccagcacc tgctggagca cctgtagcgg gtcatcagtg gtcgttgtgc gtttttcgct
     9661 ttcactaaca acggtttctt tacctaaagt ggttaaagtg caaatcggat cggcgaccac
     9721 aatatcaaag cggctatacg gatgatcggc atagccggag tgtaaaagca tcgcccacgg
     9781 caggtggctt aagcgggaga aataaaattc agcggcgtcc tgacgccagg gtaaagtaat
     9841 cacagtggga gataacgtct tcatcagtcc tgactctact ggctatgtgc cgggtagcat
     9901 gaaataatta gcgccaacaa tttagcagga gttaacaatg tttgcaggtt taccttcact
     9961 catccatgaa cagcagcaaa aagctgtcga gcggatccag gaactgatgg cacaagggat
    10021 gagtagcggc caggcaatcg cgctggtggc agaagagctg cgtgccaacc actccggcga
    10081 gctaatcgtg gcgcgttttg aggatgaaga cgagtaacac actaatccgt atcgatttcg
    10141 ttatagaagc cgatgatcat ttgattgtga gaaaaaggac gctttttata tgtgattaaa
    10201 agcgtcctga cccatttatg ctgatataaa tttaactcag accagtgctt ttagttcttc
    10261 ctgcatatat gttgaaaagt attcaggatt tttaatcaca gagcgttcac ctgtctccat
    10321 tttttttgcc catccaacca ttttttccgt gaacccaggg ttccagcctt cctggtcagc
    10381 tcgcgccgta atatcagcgg cactcgcggg tactcccagt tcttttaaat atttaaagat
    10441 tcccttcgcc gtgctttcat tcatcgaact tggtgttggc gctgatacat ccatggcacc
    10501 aatgaaatcc agggctttat ctattacagc aggcatattc ttatccttaa aattaaccat
    10561 gttatcgata aggctatata tgaccttaat ttgcatctaa tatcaaaggg attataacga
    10621 tatggcacga gttaacctgt tgacattcag gtgttttaaa ggtagcagct catggtgtgt
    10681 tatccagtaa gatggagaaa attaacggtc agcccgcacg ctataaccag attgttcagc
    10741 taagaaagaa aaccagagtt gctaatcaaa gggcgaacac aacggcatgg ttatgtttat
    10801 ttatatagtt agttttatag ataaaattga aattataaat ataaataatg aattagcaga
    10861 agcatcaaca gtaatatctt attctctggt cgatttatgt aaaaccgacc agtattatcg
    10921 ataaattatc gtctgactta ttttgcttcg ataaagctta caccgcagcc acaatcttaa
    10981 tttcaacttt atacttcggg ttcatcaaac ccgcttgtac cgtgcagcgc accggcgcat
    11041 gacccgcgac aacccaggca tcccacgctt tattcatcgc cgcgaagtcg tttttatcgg
    11101 ccaggaaaat ggtggcatcc agaatgctcg atttattgct gccctgtttt tccagcacgg
    11161 cgtcaatctg tgccagcgtg ttggcggttt gctcaaaggc atcggcgtcg aggttttccg
    11221 gtacaccagt gtagtagagc gtgttgttgt ggattactac atcagaccag cgggcttcag
    11281 catcgatacg aacgatagtc atcattttgt cctcattata cttccatttt cgtgctgcaa
    11341 agactgccac aatgtgaggt tgccgtcacc acttcaactg gcgaaagcgc cctcatcctg
    11401 acataatccc tgttcaaatc aacagggggt agtgtgacgg acgattttgc accagacggt
    11461 cagctggcga aagcgatacc aggctttaag ccgcgagaac cacagcgaca gatggcggta
    11521 gccgtcaccc aggcgataga aaaaggccag ccgctggtgg tggaagcagg aaccggtacg
    11581 ggcaaaacct acgcttacct ggctcctgcg ctgcgggcga aaaagaaagt cattatctcg
    11641 accggctcaa aagcgttgca ggatcagctc tacagccgcg atttgccaac agtctcaaag
    11701 gcattgaaat atacgggcaa cgtggcgctg ctgaaagggc gctcaaacta cctctgcctc
    11761 gaacgtctcg aacagcaggc gctggcgggg ggcgatctgc cggtacaaat cttaagcgat
    11821 gtgatcctgc tgcgctcctg gtctaatcaa acagtcgatg gtgatatcag cacctgcgtc
    11881 agcgtggcgg aagattcaca ggcgtggccg ctggtcacca gcaccaacga caactgtctt
    11941 ggcagcgact gcccgatgta taaagattgc tttgtggtca aagcacgtaa aaaagcgatg
    12001 gacgccgatg tggtggtggt aaaccatcat ctctttctgg cggatatggt ggttaaagag
    12061 agtggatttg gcgagttgat ccctgaagcg gacgtcatga tcttcgacga agcccaccag
    12121 ctaccggaca ttgccagcca gtattttggt cagtcactct ccagtcgaca actgctcgac
    12181 ctggcaaaag acatcaccat cgcctaccgc accgaattaa aagacaccca gcagttacaa
    12241 aagtgcgctg atcgtcttgc ccagagtgcg caggattttc gtctgcaact cggtgagcca
    12301 ggttatcgcg gtaacctgcg tgagctgtta gctaatccgc aaattcagcg ggcattttta
    12361 ctgctcgatg acaccttgga actttgttat gatgtggcga aactgtcact ggggcgttcc
    12421 gccttgctgg atgcggcatt tgagcgcgcc acgttgtatc gcacacggct gaagcggcta
    12481 aaagagatca atcagccggg ctacagctac tggtacgaat gcacttcgcg ccattttact
    12541 ctggctctca cgccgctcag cgtggcggat aaattcaaag agttaatggc gcaaaagccc
    12601 ggtagctgga tcttcacctc agcaacgctg tcggtgaacg acgatctgca tcatttcacc
    12661 tcgcggcttg ggatagaaca ggcggagtcg ttgctattgc caagcccgtt tgattacagc
    12721 cgccaggcgt tactctgtgt gccgcgcaat ctgccgcaaa ccaaccagcc aggttctgct
    12781 cgccagttag cggcaatgct gcgaccgatc atcgaagcta acaacggtcg ttgttttatg
    12841 ctttgtacct cgcacgccat gatgcgcgat ctggccgagc agttccgcgc taccatgacg
    12901 cttcccgttt tgttgcaggg ggaaaccagt aaaggccaac tgttgcagca gtttgtcagt
    12961 gccggtaatg cgcttcttgt ggcaaccagt agtttctggg aaggggtgga cgtgcgtggc
    13021 gatacattgt cattggtaat tatcgacaaa ttgccgttta cctcgccgga tgatccactg
    13081 ttaaaagcgc gcatggaaga ttgtcgtttg cgcggtggcg acccgttcga tgaagtgcaa
    13141 ctaccagatg ccgtcattac tctcaaacag ggggtaggtc gactgattcg cgacgccgac
    13201 gatcgtggcg tgctggtgat ttgtgactat cggctggtga tgcgtcctta cggcgcgacg
    13261 tttctcgcca gtctgccgcc cgcgccacgc acccgtgaca ttgcccgtgc ggttcgtttc
    13321 cttgcgatac catcctccag gtaatttgtt acggagtatg gtatgatgcg cgccttttta
    13381 ttgatctaag cacgaagacc catgcgaatt ctggctatcg ataccgcgac agaggcctgc
    13441 tctgtcgccc tgtggaacga cggtactgtc aacgctcatt ttgagctttg ccctcgtgaa
    13501 catactcaac gaatcttacc gatggtgcag gatatcctga ccaccagcgg aacttccctg
    13561 actgatatta acgctctggc ttacgggcgc ggccccggta gctttaccgg cgtgcgcatt
    13621 ggtattggca tcgcgcaagg gctggcgctt ggcgcggaat taccgatgat tggcgtctcc
    13681 acgctaatga cgatggcgca aggggcgtgg cgcaaaaacg gcgcaacacg cgtgctgtca
    13741 gccattgacg cgcgaatggg cgaagtttac tgggccgaat atcagcgtga tgaaaacggt
    13801 atctggcacg gtgaagaaac cgaagccgta ctcaaacccg aactcgttca tgaacgaatg
    13861 caacagcttt ccggcgaatg ggtgacggta gggacgggct ggcaagcctg gccggatctc
    13921 ggtaaagaga gcgggctggt tttgcgcgat ggcgaagtgt tactccccgc tgcagaagat
    13981 atgctgccga ttgcttgtca gatgtttgcc gagggtaaaa cggtggcggt ggaacatgcc
    14041 gagccggttt atttgcgtaa caacgtcgca tggaagaaac ttccgggcaa agaatgaatc
    14101 ttaagagtat gccctgagaa aaaaaggagt cgcatcatgg cggttcaaaa gaatgttatc
    14161 aaaggcatac tggcaggtac gtttgcgcta atgctgagcg gttgtgtcac tgtgccggac
    14221 gccattaaag gcagcagtcc cacgccgcaa caagatttag ttcgggtgat gagtgcgccg
    14281 cagctgtacg ttggtcagga ggcacgcttt ggtggcaaag tggttgcggt acaaaaccag
    14341 caagggaaaa cccgcctgga aattgctacc gtaccgctgg acagcggagc taggccgacg
    14401 ctgggagaac cttctcgcgg tcgcatttat gccgatgtga acggttttct ggacccggtg
    14461 gatttccgtg gacaactggt tacggtagtc gggccaatca ccggtgcggt tgacggcaaa
    14521 atcggcaata cgccctataa atttatggtg atgcaagtaa cgggttacaa acgttggcat
    14581 ttaacccagc aggtgattat gccgcctcag ccgattgatc catggtttta tggcggtcgt
    14641 ggctggccct atggctacgg cggatggggc tggtataatc ccggccccgc gagagtacaa
    14701 acagttgtaa ccgaataatt gcttgttttt aaagaaaaag aaacagcggc tggtccgctg
    14761 tttctgcatt cttacggtaa agataaaaat aaatagtgac gcgcttcgca accttttcgt
    14821 tgggtaatta tcaagctggt atgatgagtt aatattatgt taacagcgtg tatatcattt
    14881 ggggttgcga tgacgacgaa cacgcatttt agaggtgaag aattgaagaa ggtttggctt
    14941 aaccgttatc ccgcggacgt tccgacggag atcaaccctg accgttatca atctctggta
    15001 gatatgtttg agcagtcggt cgcgcgctac gccgatcagc ctgcgtttgt gaatatgggg
    15061 gaggtgatga ccttccgcaa gctggaagaa cgcagtcgcg cgtttgccgc ttatttgcaa
    15121 caagggttgg ggctgaagaa aggcgatcgc gttgcgttga tgatgcctaa cttattgcaa
    15181 tatccggtgg cgctatttgg cattttgcgt gccgggataa tcgtcgtaaa cgttaacccg
    15241 ttgtataccc cgcgtgagct tgagcatcag cttaacgata gcggcgcatc ggcgattgtt
    15301 atcgtgtcta actttgccca cgctctggaa aaagtggttg ataaaaccgc cgttcagcac
    15361 gtaattctga cccgtatggg cgatcagcta tctacggcaa aaggcacggt agtcaatttc
    15421 gttgttaaat acatcaagcg cttggtgccg aaataccatc tgccagatgc catttcattt
    15481 cgtagcgcac tgcataacgg ctaccggatg cagtacgtca aacccgaact ggtgccggaa
    15541 gatttagcct tcctgcaata caccggcggc accacaggtg tggcgaaagg cgcgatgctg
    15601 actcaccgca atatgctggc gaacctggaa caggtcaacg ccacctatgg gccgttgctg
    15661 catccaggta aagaactggt agtaacggca ctgccgctgt atcacatttt tgccctgacc
    15721 attaactgcc tgctgtttat cgaactgggt gggcagaacc tgcttatcac taacccgcgt
    15781 gatattccag ggttggtaaa agagttagcg aaatatccgt ttaccgctat cactggcgtt
    15841 aacaccttgt tcaatgcgtt gctgaacaat aaagagttcc agcagctgga tttctccagt
    15901 ctgcatcttt ccgcaggcgg tgggatgcct gttcagcaag tggtggcaga acgctgggtg
    15961 aaactgaccg gacagtatct gctggaaggt tatggcctga ccgagtgcgc gccgctggtg
    16021 agcgttaacc cgtatgatat tgattatcat agcggtagca tcggtttgcc ggtgccatcg
    16081 acggaagcca aactggtgga tgacgacgac aacgaagtac caccgggtca accgggtgag
    16141 ctttgtgtca aaggaccgca ggtgatgctg ggttactggc agcgtccgga tgctacagat
    16201 gagatcatca aaaatggctg gttacacacc ggcgacatcg cggtgatgga tgaagaagga
    16261 ttcctgcgca ttgtcgatcg taaaaaagac atgattttgg tttccggttt taacgtctat
    16321 cccaacgaga ttgaagatgt cgtcatgcag catcctggcg tacaggaagt cgcggctgtt
    16381 ggcgtacctt ccggctccag tggtgaagcg gtgaaaatct tcgtagtgaa aaaagatcca
    16441 tcgcttaccg aagagtcact ggtgactttt tgccgccgtc agctcacggg atacaaagta
    16501 ccgaagctgg tggagtttcg tgatgagtta ccgaaatcta acgtcggaaa aattttgcga
    16561 cgagaattac gtgacgaagc gcgcggcaaa gtggacaata aagcctgagc gttaagtcag
    16621 tcgtcagacg ccggttaatc cggcgttttt tttgacgccc actaaagaga aaacaatttg
    16681 aattaccaaa tgattaccac ggacgatgcg ctggcttctt tgtgtgaagc cgtccgtgtc
    16741 tttccggcga tagccctgga tactgaattt gttcgtacgc gcacttatta cccgcagctg
    16801 gggttgattc aacttttcga tggcgagcat ctggcgctaa ttgatcctct cgggatcacc
    16861 gactggtcac cgctgaaagc gatcctgcgc gatccgtcca tcacaaaatt tctccatgca
    16921 ggcagtgaag atctggaagt gttcctcaat gtctttggcg aattaccaca acccttgatt
    16981 gacacgcaaa tccttgctgc cttctgcgga cgcccgatgt catggggttt cgcttccatg
    17041 gtggaagagt attccggcgt tacgctggac aagagtgaat cgcgcaccga ctggctggcc
    17101 agaccgctga ccgaacgtca gtgtgaatac gcagcggcgg atgtctggta tctgttaccg
    17161 atcaccgcca agcttatggt agaaacggag gcctccggct ggctacctgc ggcgctggat
    17221 gaatgccgcc tgatgcaaat gcgtcgtcag gaagtcgttg cgccggaaga tgcctggcgt
    17281 gatatcacca atgcctggca gttacgcaca cgccaactgg cctgtctgca actgttagcc
    17341 gactggcgac tgcgcaaggc gcgagagcgt gatctggcgg tgaactttgt cgtgcgtgaa
    17401 gagcatttgt ggtcggtcgc gcgttatatg ccgggaagtt taggcgaact ggacagcctg
    17461 ggtttatccg gtagcgaaat ccgctttcac ggtaaaacgc tgctggcgct ggtggaaaaa
    17521 gcgcagacat tgccggaaga tgccttaccg cagccgatgc ttaacctgat ggacatgcct
    17581 ggttatcgta aagcgtttaa agcgattaag tcgctgatta ctgacgtgag cgaaacgcat
    17641 aagatcagcg ccgaattgct ggcatcgcgt cggcaaatca accaactgct gaactggcac
    17701 tggaaactga aaccgcagaa caatttgccg gagctgattt ccggctggcg tggtgagctg
    17761 atggcggaag cattacacaa tttattgcag gaatatccgc agtaaaatct tccgaagccg
    17821 gactgggcgc gctcagcgcc acatccggct ttggcaaact acaaatccaa caccaggcgt
    17881 ttacccttcg cacgcgaaca acagatcaac atactttgct ggctggcacg ctcttcatcg
    17941 ctaaaatact gatcccgatg gtcagcttca ccttccagta ttgccgtttc gcaggttccg
    18001 catacccctt cacgacataa acattccact ttcgcggctt tattattttc aataacctgc
    18061 aaaatagtca tctcttccgg caccacaaac tcttttccgg aacgggcaag caccagggta
    18121 aatgcatcgc cggttttgtc ttcgatagca aattgctcaa agtgcagtgt atcggcggcg
    18181 atgtccagac gcgcagcttc acttcttacc gcttcaatta gcgcctcggg gccacaggta
    18241 taaacgtgtg tgccaggttc gatatccgcc aataatcgcg ctaattccag tcgtgttccg
    18301 gttgatgaat gatgcaaatg gactttctca gcctgcggat gctggactag ctcagcacga
    18361 aatgcgcaac tttctggatt tcgcgagcag taatgtagct gccagtcgat gtcgctgtgt
    18421 tgcaactctg ccatgtgcga caggaaaggg gggataccaa taccgcccgc gataaacaga
    18481 tgctttctgg ctgagggaat tagcgcaaac aggttattag gcgttgagat cgttaaccga
    18541 tcgcccactt ttacctgctg atgcaaaaag cgggaaccgc cgcgcgagtt ttcttccagt
    18601 cgaacggcaa tctgataact agaggtgtta tgcggtgaac tcagtagtga atacgcattg
    18661 ctgtactggt tatcaccatc gctcatctgc actaggatgt gacttcctcc ggtgaacgca
    18721 ggcagcggtt tgccatcggt tgccaccagc gtgaagcgtt tcacctgttc ggtaaggggt
    18781 tcaacctggc tcacctgtac ttcaaacatt tgatagtctg acattgctgt actccatcaa
    18841 aaatcagccc agcggcggaa aataccgccg ccgatgtcat tagtccttaa acacctgcgc
    18901 cagcagatta tggaaatggg cgataccatg ttcggaaatg ccactaccgc tactgtcggc
    18961 catgatgcgc ccctgaccac gatagccacg cgatttcagc cctttctgta cgctttcaac
    19021 cagacgtaaa tcttccggat ggaacacatc gcgataccac tcaatcagcg atttttgctc
    19081 gtcggttaac tcttcattgg tgaagtaaat atcgtagttt tgcagggtag tttcagaatc
    19141 caccgggaat tcataaatga ccgtcatcat ccctttgatc ggggtgacgt tcagcatcgt
    19201 gcacggccac agccagaaac cgtggaatgc cgcatccgta ccctcttcaa atttaaacga
    19261 ctgttcggac ggtttggcga aaccgtattg cagcgtccag ttaccgtgca tggtgtgcca
    19321 gtaacgatca acctgtacgg agtcggagaa acctagatgc gccggaccac agtgatagca
    19381 ctcgagatag ttatcgacaa tgttcttcca gttggcaggc gtgcgggtgg taaagcgggc
    19441 cgctagtttc agattgtgga cttccgggca ggcttccagc actttcgcgc ccagaccggt
    19501 gtattgattc acttgaagta cgaaaaaaaa ccgggaggac attggattat tcgggttctg
    19561 atgggattag atttggtggg gcttgcaagc ctgtagcgca aattttagtc gttaatcaat
    19621 gaaacgcgaa agatagtaaa aaattgcttt tgtttcattg aaaatacgaa aaacaaaaac
    19681 actgcaaatc atttcaataa cagcttcaaa aaacgttcaa aaccgataac aaccaggctg
    19741 t
//
LOCUS       DAJTUN010000077        19697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTUN010000077 DAJTUN010000000
VERSION     DAJTUN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.077"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..3520)
                     /gene="mukB"
                     /locus_tag="OO470_002528"
     CDS             complement(<1..3520)
                     /gene="mukB"
                     /locus_tag="OO470_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6251743.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRM"
     gene            complement(3520..4224)
                     /gene="mukE"
                     /locus_tag="OO470_002529"
     CDS             complement(3520..4224)
                     /gene="mukE"
                     /locus_tag="OO470_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6251744.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4205..5527)
                     /gene="mukF"
                     /locus_tag="OO470_002530"
     CDS             complement(4205..5527)
                     /gene="mukF"
                     /locus_tag="OO470_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6251745.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(5524..6309)
                     /gene="cmoM"
                     /locus_tag="OO470_002531"
     CDS             complement(5524..6309)
                     /gene="cmoM"
                     /locus_tag="OO470_002531"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6251746.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            6445..7224
                     /gene="elyC"
                     /locus_tag="OO470_002532"
     CDS             6445..7224
                     /gene="elyC"
                     /locus_tag="OO470_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6251747.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7201..8094)
                     /locus_tag="OO470_002533"
     CDS             complement(7201..8094)
                     /locus_tag="OO470_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6251748.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8248..8994)
                     /gene="kdsB"
                     /locus_tag="OO470_002534"
     CDS             complement(8248..8994)
                     /gene="kdsB"
                     /locus_tag="OO470_002534"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6251749.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(8991..9173)
                     /gene="ycaR"
                     /locus_tag="OO470_002535"
     CDS             complement(8991..9173)
                     /gene="ycaR"
                     /locus_tag="OO470_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6251750.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9225..10457)
                     /locus_tag="OO470_002536"
     CDS             complement(9225..10457)
                     /locus_tag="OO470_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6251751.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(10494..11480)
                     /gene="lpxK"
                     /locus_tag="OO470_002537"
     CDS             complement(10494..11480)
                     /gene="lpxK"
                     /locus_tag="OO470_002537"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6251752.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(11477..13225)
                     /gene="msbA"
                     /locus_tag="OO470_002538"
     CDS             complement(11477..13225)
                     /gene="msbA"
                     /locus_tag="OO470_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6251753.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13262..15521)
                     /locus_tag="OO470_002539"
                     /pseudo
     CDS             complement(13262..15521)
                     /locus_tag="OO470_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(15728..16012)
                     /gene="ihfB"
                     /locus_tag="OO470_002540"
     CDS             complement(15728..16012)
                     /gene="ihfB"
                     /locus_tag="OO470_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6251754.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16172..17845)
                     /gene="rpsA"
                     /locus_tag="OO470_002541"
     CDS             complement(16172..17845)
                     /gene="rpsA"
                     /locus_tag="OO470_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6251755.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(17956..18639)
                     /gene="cmk"
                     /locus_tag="OO470_002542"
     CDS             complement(17956..18639)
                     /gene="cmk"
                     /locus_tag="OO470_002542"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6251756.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(18816..19577)
                     /gene="ycaL"
                     /locus_tag="OO470_002543"
     CDS             complement(18816..19577)
                     /gene="ycaL"
                     /locus_tag="OO470_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6251757.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4745 a         5452 c         4781 g         4719 t
ORIGIN      
        1 acatgcgcag cacgtcgcgc agatgttcgt tatccgccac cgccagacgc agcgcaccta
       61 acgccttatc cgacatggaa cgcaaatcat cagcggagag ataagccagc tcacgacggt
      121 gtaagcggcg ctcaacgccg ttatctttca ccatgcgcat caccgcacac cagcccgctt
      181 tggcggtcac tacctgctcg cgcatctcaa agtaatcccg ctccagcttg cgcagtttgc
      241 gggtcaggtt gtccatctcc gcttcgcaga aggtaagcgc tttttccagt tgattgcggc
      301 gtgaacggtt attgctcagt tgcgcatgca gctcgtcacg gcgaatacgc gcccgctctt
      361 ctgccccgct atcagcacgc acgccgatat cctgcaattc acgttgcaga tcgttgagta
      421 gctctttttt ggtgtcgtaa gaacttttca gcgaagccag cacctggttg tactgactca
      481 actgcgctgc gtgaccgcgc aacgcttcgc gagcgcgggt acgctccgct tccgcctgtt
      541 ccagacgttc acgcagtttt tcgttgagat cgctgttacc gctaagcatt tctgccgagt
      601 cagaatagct aaagtgcgca cgacgctgca ccacttccgt cagggcaaac gcttgctgac
      661 gggcatcgcg ctgcatctgc tgagagtacg cgtaatcttc ttttaactgt tcgaactgtt
      721 ccgggtcgct ctgcaatacc gaaacgatcg gttccagttt cgccagttga ttgccaaact
      781 gctgaacaaa acgcgcggct tcctgggctt catccagacg ttcgcggatt tcatcgacgc
      841 gatccgccag gctgtcatca gccaacaggt tgagacgcgg cagaatgcgg ttcagcgccg
      901 taacgccttc tttcgcctgc tcaaactgaa tacgctgctg ctggttatca ttttcatgat
      961 tactcaacgc ccgctccagt tcgactcgac ggctgttcag ttgacggatt tctgcttccg
     1021 ggtcagactc aaacgccacc gccagatgac tgccgataaa gcggctgaac gcctgatgca
     1081 gacgctgggt tttctgtaca tcaaaggaga gcgtggcgaa gcgttcggaa agcacttcac
     1141 gctcggcatg gaggctttca atacggcttt cacgcgcagc acgaccaaac agcggcactt
     1201 ccgggaaacg tgaataacgc cactgtcgat cggcgatttt caccaccacc gctttttcca
     1261 gctcatcaac gctgaacacg ctatcatcga atgactgcgg atccccttcg atcaaataga
     1321 gatcttccgg gcaatcggtc aagccttcca ggtgttcggt tacctgtgac agatctggca
     1381 ccacgatggc gtggcgtgac gggccataca gagccgagaa gtacggcgca tcttccaggc
     1441 taacgtcgtc ataaatttct gacagcagca caccaccaaa acgctccgcc agcgcgttca
     1501 gacgctgatc ttcagagccg ccaggctggc ttaaacgttc gatctcttca tcgacggcgt
     1561 ttttgcgcgc gcctacttca tcgcgttcaa caatcgcctc gcgctcacgc tccagcaact
     1621 gttgcagata ttcagtgacg tcctggctgg aggagaactc ttcgccgcac tgttcactca
     1681 actggttgag actgttttgc gctgccagcc aaaccggtgc acgctgcatc aaactctgaa
     1741 tgcgagactg cagctgttcc tgttcctgcc gcagtgccat ccgctcttca cgggcgttag
     1801 atacgctatc ggaaagagag gcaatgcgtg cttccagctc ctgatgcaga gcttccagtt
     1861 catcaatatc aaaattcttg ccctgacgtt tgcagaaatc tgccagcaga cgctcagctt
     1921 cttgctgctc acgcagacgc tgttccagtt cgcttaagcg catccgcaac ggctgaacct
     1981 gctctgccag gtgacgctga tcaacccctt cgcgcaatag ttcgcgagcg acatcccacg
     2041 cctcgttacg cgccagcggg ccgttgattg ccaccaccag ctgataagcc tgctcaaact
     2101 ggctgtgcgc ggtttgcgcc atgctcattt tctgctcaag agagagcatt ttttcagtcg
     2161 cttccagctc tttcgcctgg aaggtttcca gccattcggc ggcgctgtcg gcggttaagt
     2221 ccggcagatg gcacagttct ttggcacgat taagcgcagc aatcgcctgg ttatactgga
     2281 tcgcgcgcgt ttgctggacg tccagcgcct gctggtagtc agcaagctgg cttttcagct
     2341 cgtccacttc cagttcggca gcttccgcgc gagcctcatt ctcttcctgg cgttcgatgg
     2401 cttctgccac cacttcattt tgctcttcca gacggatctg cagctcatcg agatccactt
     2461 cgtagcgttc gatcttctcc tgctgacgca atgcggtttg caccaggttc aggtgatcgc
     2521 tggccgcctg ataatccgct tccagatcgc cttcggcacc gttgtgctct gccagctcac
     2581 gcgccatatc gacgtgtttg tactgctcag ccgccagttg ctgacgcgaa gtatgtagct
     2641 cgcgacgaaa ctccagggct ttgtcgagat ggacacggcg ctcgttggcg tgacgcatgt
     2701 agtccgccgc cacgtagttg gtagcttcgc tgatcagatg cttaaacagg tcacggtccg
     2761 actgggtgac acgaatcgct tccagcgtca tgcggttttc acgcagcgcc gcttccatgt
     2821 cctggaacgc tttacgcacg ccgctgtttt ccggcaacag gtagtcgcgc agagaacggg
     2881 taatggcact ggagatcccg ccatacagcg aagcttcgat cagacgatag aatttgctac
     2941 ggtcagatgc ggagcgcaga cgacgcgcga tgatgcccag atcgaacatc agcgagtggt
     3001 aatcggtaat ggagttgaac tgtttaaact gcacgccttc catcgcctcg agcttgtctt
     3061 tcagctcgtt aagcggcagc acgcgcgcct ggcgttcgtt cagggtttcg gtcaccagct
     3121 gtgtcggctg caccgacatc ggcagtccct gaatggcaaa tggtttgata tccactttac
     3181 gatcgcgtcc ggcaacctgt tgcagacgca caccgaccac cacgcgctgg tggcgcgagt
     3241 taatggtgtc gagcatcgaa taacagacac ccgctttcag cttaccgtgc agacctttat
     3301 cgcgcgaacc gctggtggcc ccggcttccg tagtgttacg gaaatgcagc agggtcaggt
     3361 cggggatcag cgccgtaacg aacgccgcca tcgtggtgga tttacccgcc ccgttaccgc
     3421 cggaaagcgt cgtgaccagc tcgtcaaggt caaaagttcg ggcaaaaaag ccgttccagt
     3481 taatcagcgt cagtgagcga aatttaccgc gttcaatcat tattcttcct ctccgctatc
     3541 tggctgactc tcttcggttt catcgttgag ttgcagatga ttttcaatcg gcattgcttc
     3601 gccatcacga atcaggcgac gctgtgcttc acggggatcg tcgccagcac gcacatcggc
     3661 tccgaagcgg aacaccgatt cggtaatgcg gaacttgctg ctgtcgtggc ccataaacca
     3721 caccatgcct aaacgacgca gacggttgag cgaagaacgt actttctcct gcaacttctg
     3781 acggtcaacg tctgaaccgg ttgaacggtt gttcaccagt ttcagcagtt tcgcttcatc
     3841 ggccagggtg agcagttcgt cgtacagttc ctgctgggtg aaaatcccct cattcgccag
     3901 ccgttccggg ctgagataga gataacagag gattttcccg accatcatat ccagttccga
     3961 caagacggaa cgcgggataa gagtggtgga acgtgggcgt aaatagaaga acccttccgg
     4021 tgcgcgaata agctcaacgt tataacgcgc gtaaaactct tccagatatt cctgaaaatc
     4081 catcaggaat gcatgattat ccagttcgtc gaggccaata tggcggcctg aacgtaaggc
     4141 gctgtccagc gccggaaata acggattcgc cagcgcctgc gccagcttaa ccggcatcac
     4201 ttgttcaata tttgtcgatg acatgcgcct gtaccttggc tccgtaatca ttaatcggct
     4261 gccatttcgc tggcagtccg gtgaaatctg cttgcgctac gccaagacgt accgcctgat
     4321 caataacaat acgcgcaacg tcaaagtgac gtgcacgcgg atactgtgac agatattcgc
     4381 gtaccaccag accaagatcc agcggcactt gtctggtttt gtacacggca agttgttctt
     4441 cgatgatcgc cgccagctgt tcgcggatct cgttaaactc ttcgtattcc agatcctcag
     4501 gaagttcccc agtcacttct tcatcgcgca gtgccatctc ttcgtcacgc atatccagca
     4561 gacgatcggc attggcataa gttagcgccc acggctcatc aaaataggtt tgtaccgact
     4621 gacgtaaccg ctgagcaaag acgcggtttt tatccatatc gatcgcggta cgaataaatt
     4681 tgtgtacgtg gcggtcgtag ccaatccaca agtcgatgga ttgctggccc caactgataa
     4741 tacgatcgag tttgctctgc agatcgaaca ccagacgatc gacgaaatgc agatcgtcat
     4801 gggtcatcgt cgcatcctga atgcgcaaca gattagcctg caatttgtcg cctgccgctt
     4861 ccagcgtatc ctgcaattca cgcagcgttc cggaagtttc cgaaagcaac aattcacagc
     4921 tggaaatcgc cgcccgccag tctttgttca gcaactgggc gatatcgtcc ttcacctgct
     4981 gctgctgttc gtccatcaga cgttgcgtca ggtcgatact gtcgaaaatt tctgctaccg
     5041 aatatttcag tggcgcatag acattacggt gccagtgaaa ttcatcaccg ccctcttcgg
     5101 cggcatctgc tgcgcgtttg agctcacccg ccacaatcga caactgcata gaaagacgca
     5161 gcgtagaaaa ctcgcgctga cggatgtagt agtcagtaat gccgatgccg agcggcgtca
     5221 gacggtaaat tgcgttccct tccgcctgct cgctggtaaa gcggttcagc agacgttgac
     5281 gcaccatgtc gttgatcgcg ttattggcgc gcacgccgat ggtttcgctg gtttgctcaa
     5341 acgcatcact cacatggcgg aatgcatcca ccagctcgcc ttcactcatc tcaccatcca
     5401 gacgctcgcc gttcagcgtg gcaaccgcca gcagaaaaga gagtcggtct accggcagcg
     5461 agatggagaa gtcatttttt ctggcccagg caaccagttc ggggactgtc tgggaaaatt
     5521 cactcatact ttatccttgc tctgcggttt gcgcgcggtg acatgaatat aacgccctag
     5581 ggtaatatac ggttcctgac ggcaataacg cgtttctaat tcaagtaatg cttcatagca
     5641 gtcgcgctgc tggtacttct cgcgcagata atcatgaaac acgcgaacgc ctgtcttacc
     5701 cataatttgc caaccagctt cttccagcca cagataaacc tgcgccgggt cgcgtggata
     5761 atctggcgaa agcgtccgtt ttttcttttt cggcattccc gcctgcacgt aatcaaaatt
     5821 cccggcgacc atgttatgca tcaacaaacc atgcgcattg tagaacatta acgacaacac
     5881 tccgcctgga cgcaacactg accacagggt ctgcaataca ctgcggggat cggccaccca
     5941 ctcgagcacc gcatggaaca atatcagatc aacgggcgtt tccaaatgcg aagcaacatc
     6001 ctgagcggcg caatgtataa attgcatgtt gtcgctcaca ccttttgctt ctgccgcctg
     6061 ttttgcgcgg tcgatcatct gcgcagaaag atcgcataaa ataacctgat gcccgcgctc
     6121 ggccattttg attgcggtct gcccttctcc gccgccagca tccagcacac gcagtttttg
     6181 cgggcccatt tccgccagca cgcgatcgag atcctgccac agaatagcct gtcgaagctg
     6241 ccctttggtg gtgccgtaaa tgttacggga aaacttttcc gcaatatcat caaaattgcg
     6301 atcctgcatt gctttctcca ccggttggct aagacgtttt ctgataaaga cgctattttg
     6361 tcacagccac ggcgagaatg aacccgtctg gtgtaatatc gctagcgatc ccttgctata
     6421 tggttaaaaa aggaaccaga aaggatgctt tttacactga aaaaagtgat tggcaatatg
     6481 ttgctgccac ttccgctgat gttgttaatt attggcgctg gcctggcgct gctgtggttt
     6541 agccgttttc aaaaaaccgg gaagattttt attagcattg ggtggctggc actcttgtta
     6601 ttgagtttgc aaccggtagc cgaccgcctg ctgcgtccca tcgaaagcac ctatccgaca
     6661 tggaataatt cgcaaaaagt ggactatatc gtggtgctcg gaggtggtta tacctggaac
     6721 ccgcagtggg caccgagctc taatttaatc aataacagtc tgccacgcct gaatgagggt
     6781 atccgtttat ggcgcgaaaa tccgggttca aaactgatct tcacgggagg cgtagcaaaa
     6841 accaatacgg tgagtacagc ggaagtaggt gccagagttg cgcaatcgct gggcgtaccg
     6901 cgcgagcaaa ttatcaccct ggatttgcca aaagataccg aagaagaagc tgcggcagtg
     6961 aaacaggcga ttggtgacgc ccctttcctg ctggtgacct ccgcatcaca tctgccgcgg
     7021 gcgatgatct tttttcagca ggaagggtta aatccgctac cagccccggc gaatcagttg
     7081 gccatcgact cgccgcttaa tccgtgggaa cgggcaatcc cttctccggt ctggctgatg
     7141 catagcgatc gtgttggcta tgaaacgctt ggccgcatct ggcagtggct gaaaggtccg
     7201 tcaggcgagc cacggcaaga gtgattttga tgccagatcg aagttgcgcc gactaaatcg
     7261 tccggtatta accagttgcg caacttcatc ccataacaca tacaaccaac gccgccagat
     7321 gaacgactcc gccactggcg cgcgttgcag gtaactccag agcaaatctt ccgccagaga
     7381 attatccatt agtcggaaca gttcgtattc tcgcggtgcc caaagcatta gtcccggccc
     7441 gaccatcgcc agtaactgat cgctgcgcga atctttcagc atgctgcgta aacagaagtt
     7501 accgtgaatc aacacacaat tgtcgttaaa gccttcaaat aatgccggga gacattcgcg
     7561 agtgcgaaac aggatccgct tatcctgcat cgtcagaccg gtgttattga actgattgag
     7621 cgtggtccat agcacttcaa catgttgccg gtaccatgag ggccagaaat tttcctgagt
     7681 attgtcgacc gcgccgacgc aaccgcgact gtcctgacgg tgccaggcca gtaaggcttc
     7741 aacgatttgg tctttgagct gttcccagcg ttctggtgtt cgggctggtg cctccaccga
     7801 aacaccacgc atccgctcca gtagcaggac atcagggccg gggtgttctt catgcgtcat
     7861 cacaccataa atagtcggca tacggacagt cccacttctt gccagcatgg tggttttcca
     7921 tgccagttgt cgggcttttc cgggcgtact aaagcttctt gccattaacg gcattgggtt
     7981 tccctggctg tcatacaaag cccacaacgc cgtatccgct ttttcattga cgcactcaat
     8041 acgactgagt ttttcgccca gtaaatggct taattcggca cgcagctgtt ccattcagat
     8101 taccctcata aaagctactg ctttcataat gagcgccaat ataaaagatg tcacctgaat
     8161 gagatcaatt gttgaaaaga aaaattgagg aaaatcgtaa cggtacgaca ctcctcccaa
     8221 aattggctga agtgtcgtga agtgaaatta gcgcatttca gcgcgaacgc gctcaagatc
     8281 ttcaggggta tccacacctg tgccaggaac ttcctgagca acagcaacat ggattttttc
     8341 gccgtaccac agaacacgaa gctgctctaa catttcgatg tgttctaacg gacttgcctg
     8401 ccagttgacg taacgacgga taaagcctgc acggtagcca taaataccaa gatgacgcag
     8461 gaagttatcg ccaacggttt caaggccttc tgcaaaacga tcacgatccc aaggaatggt
     8521 ggcacgtgag aagtacagtg catacccttc agcgtcgaga accactttca ccgcattcgg
     8581 gttaaacgct tcttccgcat tgtggattgg caccgccaga gtcgccatac ccacctgacg
     8641 ctgagcgagg ttatcagcaa cctgacgaat gattgtcgca gggatcatcg gttcatcacc
     8701 ctgcacatta acgatcaccg tgtcgtcgct gaatgcgcat ttttcgacaa cttccgccag
     8761 acgttctgtt cctgactgat gatcggcgcg cgtcatacat acttcaccgc cagcggcttc
     8821 aacggcgcgg gcaacatcat catgatcggt tgccacgatg atgcgctcgg cacctgattc
     8881 acgcgcgcgt tcaagaacat gaacaatcat gggtttgccg ttaatatcaa ccaatggttt
     8941 accgggcaga cgcgtggacg cgtagcgcgc gggaataatg accacaaaac tcatgattta
     9001 ctctcatcag cagtcagcac gcgggcttcc gtttccagca acactggaat gccatcgcgg
     9061 agcgggaaag ccaggttatc cagtttgcaa ataagctctt gtttttcctg gttataccaa
     9121 agttttccgt tgcaaaccgg gcaggcaatg atttcaagca aacgatgatc catagttcct
     9181 ccagatggat cgggttatga atgcataaat cttatcataa tcatttatgc gacggggtct
     9241 atttcccagc cagtgcggca atcagtaaag agaccttgcg ggcagcgtcc taatgtcacc
     9301 cgcgttgcct gctgccagct cgcgaaatca gtaatcgcct gacgtaaccc tttttgcagc
     9361 attgtcgttg gtttaatgcc ttcctgtaac cacagagaga taacttcaag gatgcctgtc
     9421 tggcgatgca ttttggcatc cattcgccca actaattgcc cacgatgtaa taatggcaga
     9481 acaaaatagc catactggcg tttcggcgct ggggtatagc actccagccg gtagctaaaa
     9541 tcaaaaagct gctctgcgcg tttgcgatcc cagacaacag gatcaaaagg cgaaagtacc
     9601 gcgctgtgcg ttgcagtgag ctttccggcc agcgcacgct cgagtagcgg cagcaaatca
     9661 gcatgcagcc aaagattgcc caatttttca acatgcacag caatgatttg ctgctgttca
     9721 gccctcgctt cgcgccacgc cgccagtgcc ggacgtttca gccgatagta atctgccagc
     9781 cactgttcgc ggaatattcc caggctacgc gcactgttat ccagcatgat gatttctgct
     9841 tctgtttgcg aaacgagatc gcgctcatca tcccagtcag gcatgacacg gtgggttaaa
     9901 tcataaacgc gctggaagtt gcgccgttca atcaccatca cctttccggc agtaaataaa
     9961 ccttccagat gccgtttatg cggcttccat tcccaccagc cgcttgcacc tttacgagga
    10021 tgctcaaaat cggctgaacg taccggccct ttatcatgaa tatgctgaat taactgtgca
    10081 atttccgcct catgttcctg catccaggcg tctttgtatt tccagcccat tttttcaggt
    10141 gccagcatgc ggtggcgaat aagacgaaag tcgctacgcg gcataaagca ggcttcatgc
    10201 gcccagtatt ccattaattc gccacgcgcc agagactcat ccagccactg ggcaggataa
    10261 tttcccagac gactgaaaag caccagatat ggactacggg caacaatatt gatggtatcg
    10321 atttgcagca aggacatgcg ggagatcgtt gccggaatat cctccaacga cgctcgacgg
    10381 cggggtttgt ttaacaggcc ttgtgcggca aggtgaagat tacgcgcatc agcaagggag
    10441 aggtgcggca gcgacattca tgactccatc aatcgaacgc tgccgcggcg taactagttg
    10501 ccagaagcca gcgaggttag ttgcgtaagc agtttcgctg gttcatcacc tgaaagctgt
    10561 gcgtctacag gcaaatacca ccaattttct tctgcaaagg cccggcattt caccgcatct
    10621 ttttcagtca ttaccagcgt ttgcccggcg cttaccaacg cactgacatc cgcatggttc
    10681 aaagactgat gatcggccag cggtacacat ttttccggtt gtacgccaca catcttcagc
    10741 gtggcaaaaa agcgcggcgg atgcccaatc cccgccatcg ccactacatg ttcaagctga
    10801 gcaacgtcac aacgcgtacc ggtacgtaaa ttcaccgcct gacccggcag cagatgcatg
    10861 gggatttcac cgctgcgagg gacaccgccg ttgacgatta ccgcatcaac cgactttaag
    10921 cgccccgctc gctcacgcat tggccccgcc ggcaaccacc agccattgcc aaagcgacgc
    10981 acaccatcaa taacgacaat ttccacatca cgcgccagac ggtaatgctg taaaccgtcg
    11041 tcggttacga tgatctgcac atcagggtgt tgcgccagaa tggcttttac cgcatcagaa
    11101 cgaacgggag aaaccgcaac aggcgcatca gtgcgttgat aaatcaacac aggttcatca
    11161 cccgcctgtg ctgtggtggt atctgccgac aataacagcg gataagattc agccttacca
    11221 ccatatcccc gcgatacgac ccccacgcga ataccgcgct gttgcaactg ttccaccagc
    11281 cagacaacga ccggggtttt tccgttgccg cctgcggtga gattaccaac cacgacaacc
    11341 ggtacggggg cacgccaggc gcgcttcagt tttagtttat agcaaagacg gatcgcgcca
    11401 ctcaccaggc catacaacca ggagagtggc agcaatagcc gccacaaagg ggattcacca
    11461 gaccagattt tttcgatcat tggccaaact gcattttgtg aagttgcgcg taaacgccgc
    11521 ggtgctcaag caaatcgtta tgcgtaccgc gttccacaat gacaccatcc tcgacgacca
    11581 cgatttcgtc tgccttttca atggtagaca agcggtgggc aatcaccaga gaggtacggt
    11641 ttttctgcaa ctcatccagt gccgcctgaa tcgcacgttc ggattcggta tccagagccg
    11701 aggtagcttc atccagaatc agaatcgggc tatcacgcag caaggctcga gcgatagcaa
    11761 tacgctgacg ctgaccgcca gagagcagca cgccgttttc accaatcact gtatcgagac
    11821 cgttatccat cttattgatg aagtccatgg cgtaggccat ccgcgccgct tcttcaattt
    11881 gctcacggct gtactgttca gtccgtgcgt aagcaatgtt gttagcaacc gtatcgttaa
    11941 acagatggac attctgcgac accagagcaa cctggttacg taacgacgcc agggtatact
    12001 cgcgcagatc gtgaccatcc atcaggattt cgccttcatc aatatcgtaa aaacgcgtga
    12061 tcaggctggc gatggttgat ttacctgaac cagagcgtcc aaccagagca accgtcttcc
    12121 ctgccggaat tttcaggttg atgttacgca atgcgggtac gtcacgtccc ggataagtaa
    12181 aggtgacatt gcggaattcc acgtcgccag tcgcacgctc gatcacgcgc ttaccttcgt
    12241 ctttctcctg ctcactgtcc agaatggtaa acagcgtctg acaagccgcc ataccgcgct
    12301 ggaactgggc gttaacgttg gtcagcgatt tcagcggacg catcagtgca atcattgaag
    12361 agaaaacaac ggtaatcgta ccggcagtca ggttatccat aacacttggg aagctcgccg
    12421 catacagaac aaacgccagc gccaaagagg cgatcagctg aatgatcgga tcagagatgg
    12481 aagaggctga aaccattttc atcccctgaa gacgcattcg gttactgact ttatcgaagc
    12541 gtttcgtttc cacttcctga ccaccgaaaa tcaatacttc tttgtggccc ttcagcattt
    12601 gttctgcact ggtggtcact tgccccatgg tgttctgcat gtttttactg atgttgcgaa
    12661 aacgcttcga tactacgcga atcgcaatcg aaacaatcgg tgccagcaca atcaaaatga
    12721 tcgacagttg ccaactgtaa tagaacatca tgatgaacag gccgatgatc gacgcacctt
    12781 cacgcacaac agtaatcagt gcgccggaag aagaagaagc aacctgttcg gaatcgtagg
    12841 tgatacgtga caacaacgta cccgttgact gtttgtcaaa gaatgaaact ggcattccca
    12901 tcatgtgacc aaacaggcgg cgacgcatgg tcattaccac ctttcctgat acccaggaga
    12961 tacagtagct ggagacatag ctggtgatac cacgtaaaat catcagtccg atcaccacca
    13021 gcggcatcca caccagcacg gagcgatctg ttttaccaaa gccatcatca agaagtggct
    13081 taaggagcga taacatgaag gtatcgctgg ctgcgttgag gattaacgct acgcccgcca
    13141 cgatcagacc cgctttgaaa ggcgcaatgg ttggccacag tcggcggaat gtctgccacg
    13201 tagagagatc tttgtcgtta tgcattcaaa aaaccagcat ttgttgaaat agccgcatat
    13261 tctacccgtt atccactggc acgccaaacc actgatgata ccaacgaggt aaaatttgct
    13321 ctctgaggct gctaatccgc cagccttgtg ctgaaaaatt gaccgtcact tgaccctgat
    13381 gtggagtatc aagccattga tatcctttct gttgatagcg atgcttaact ttgttagagg
    13441 gcaatcgcca tgcgttatag cgcgatgccg atgcgagtgc cacttttcca ttcactcgct
    13501 gaattaatgg caacgatgat gaggtattac tgccatggtg aggtacctga agcaatgttg
    13561 cctgcacttg ctgccagtaa cggcttagca tcttttgttc agctggggct tcaatatcac
    13621 cggttagaag aatgctattc gtcccgtcat caaccttaac cacacaggaa tggttatttc
    13681 ccttatcgtt gctaccttgt aaaggccagt gcgcgctgaa acgcaatcct tgccactgcc
    13741 acgcttcgcc acgcacacag ggctgatgat gttcccagtt taacggactt ctgatccata
    13801 acatcggcca tatatgcaat attgaatcca gccctccccg gtgatccaga tgttcatggc
    13861 tcagaataac gccttccggt tcaagattat gccagtggag ccaggggata atcagttgtt
    13921 gcccactatc cccttcaggc cagtcctgtg tcatagagaa tcgctttgcc gtttctggct
    13981 atcaccattg ccagcccttg cccgacatca agcatgtaca gctgccactc gtcaggtcga
    14041 ggtttttgcc acagcggcca gcacatcagc aagcctccag ccacacacat tgctggcaac
    14101 gttcgccagg cgtttaatcg ccataccacc agtaagaacc atggggaaaa tgatagccat
    14161 tgccaacatt caacaatgtt gatccaccct tccggcaaac tctttaaccc ccagaaaagt
    14221 aaagccaaag accggtcggc aagaaaccat aacccctgtt ctaggattaa cggcccgcta
    14281 aaatgcacaa ccatcgcggc gaggatcaac ggaaccgtga taaatgtcac caagggaatt
    14341 gcaaatagat ttgcaataaa cgaggtcaga ctaatgccat gaaatatgac gatttgcatg
    14401 ggcataagca ggagtgtgat tcccagttgc agatggatga gggaaacaac tgcacgcaat
    14461 accggcggca gttgccactc aggacaggga aaccactgat accaaaaaat cagtgccgcg
    14521 accgcagcgg cagagagcca taaactttgc gagagaatgg caacaggatc catcagcaaa
    14581 attgccgcca gacaacatat ccatacatcc cagccactcc actgtcgccc acttaactta
    14641 agcattcccc acgtagcaag cgccaccacg gtacgcaatg caggaggttg cattcccgtc
    14701 aaccaggcat aaaaagcagc acagcagatt ccgccaatta atggtatttg ccagtggatc
    14761 cagcgcccag gcagaaaaat ttgtccactg cgaatgagtc cggcagccag caacgccgca
    14821 aaagcgatgt gcaatcccga tatcgccatt aaatgcgccg ttccagtatc gcgcatgata
    14881 tttttgattt ctttggggac ggataaccgt tcccccatac ctaaaccaag aataaccgca
    14941 ttccacggat agggttgcag cgttgtttgt agtgacgcca gatactgtgc acgcaggcta
    15001 caattcggtt caatgacact tgcctgcaga aaacggccgg tgagcggctg atgctgggca
    15061 atggcataac gctggctatc aaagccgcca tcattaagtt ggccatgaac tgcacgaact
    15121 ttgagtttca ttgaccattg ttgtccagca caaaccgctt gcggaagtta ttcgccatac
    15181 agcacaaggc ctggcgcagg gaatatacgt cgaccttgta gataagtaat ttgaccgtaa
    15241 tgagtggtca tgccgtcagt tgcagtgatc tcaacaattg catcctgcgt cgcgccagtt
    15301 aaggtttctc ctgcccacaa aatttgcttt gctgataata tgccccaaac gaaaaacagc
    15361 aacgtcagcg cgatataacg gacggtttta acagggataa atgccagtac gcaggcgaag
    15421 agagtcagaa acgcaagggt taatgtccta ggcaattggg gcaaaatcag caacggaaaa
    15481 attccgcaaa ttatgcatac gccgactgtc gttattttca tgcacccctc cctgtttatg
    15541 gaaagtgtgc aactttgttg agcggttgtc tggtgggaat tttgcgttcc ccgaggcaca
    15601 ttcagaagaa tttaacgaaa gggtgaaaac tgctcaaaac tctgcgaggc agcttccagt
    15661 tcctgattag agaattgaaa aaagcacccg acaggtgctt ttctcgcgtt caagtttgag
    15721 taaaaactta accgtaaata ttggcgcgat cgcgcagttc tttaccaggt ttaaagtgag
    15781 gaacgtattt tccttccagt tctactttat cgccagtctt cggattacgt ccggtacgtg
    15841 gtgcgcggta gtgcaaagag aaactgccga aaccgcggat ttcaatacgc tcgccctgcg
    15901 caagagtcga ggccatatgc tccagcatct cttttactgc atcttcaacc gtcttggcgg
    15961 gaatgtgcga ttgctgggtg gcaagtcttt ctatcaattc tgacttggtc atgattcctc
    16021 cggttcctta aaggcaaatt aagcggctgc tgttgctgca ttgattaagg gcggccgtag
    16081 ccgcccttag tgcttgatta caggacgaaa cctgcaatct gtcaagtaaa ctcaacaaac
    16141 ttcggaataa aaatcccgaa gagtcagaga attactcgcc tttagctgct ttgaaagctt
    16201 cagccattgc gttgttggag aagtttgcat cttcctgttt gttaacagtt gcgattgcat
    16261 ctttctcgtc agcttcgtct ttcgcacgaa cagacaggct gattgcgcgg tttttacgat
    16321 caacgccggt gaatttagct tcaacttcgt cgccaacgct cagaaccagg gtagcgtctt
    16381 caacgcggtc acgggatgct tcagaagcac gcaggtaacc ttcaacgccg tcagccagtt
    16441 ctacggttgc gcctttagcg tcaactgcag ttactttacc ggttacgata gcgcctttct
    16501 tgttcagagc aacccagttg ttgaacggat cttctgcgag ctgtttaacg cccagggaga
    16561 tacgttcacg ttctgcgtca acctgcagaa caactgcagc gatttcgtcg ccttttttgt
    16621 attcacgaac tgcttcttcg cctgcaacgt tccaggagat gtcagacagg tgaaccaggc
    16681 cgtcgatgcc gccgtccagg ccgatgaaga taccgaagtc agtgatagac ttgattttac
    16741 cttcaacacg gtcgcccttg ttgtgggttt ccgcgaactg ctgccacggg ttagctttgc
    16801 actgtttcag acccagggag atacgacgac gttcttcgtc gatatccaga accataactt
    16861 ccactacatc gccaacgtta acaactttgg acgggtggat gtttttgttg gtccaatcca
    16921 tttcggaaac gtgtaccaga ccttcaacgc cttcttcgat ttcaacgaag cagccgtagt
    16981 cagtcaggtt ggtcacgcga ccagtcagtt tggtaccttc cggataacgt ttagcgatag
    17041 ctacccacgg atcttcgccc agctgtttca ggcccaggga tacacgggta cgttcgcggt
    17101 cgaacttcag cactttaaca gtgatttcgt cgcccacgtt gacgatttcg ctcggatgct
    17161 taacgcgttt ccaggccatg tcagtgatgt gcagcaggcc gtcaacgccg cccagatcaa
    17221 cgaatgcacc gtagtcagtg aggttcttaa cgataccttt aacttccatg ccttcctgca
    17281 ggttttccag cagctgatcg cgctctgcgc tgttttcgga ttcgataacg gcacgacgag
    17341 aaacaacaac gttgttgcgc ttctgatcca gcttgattac tttaaattca agctctttgc
    17401 cttccaggtg cagagtgtca cgcaccggac gaacgtctac cagagaacct ggcaggaacg
    17461 cacgaatacc gttcagctca acagtgaagc cgcccttaac tttgccgttg ataacaccgg
    17521 taacagtttc agcatcttcg taagcttttt ccagcgtgat ccaggcttcg tgacgtttag
    17581 ctttctcacg ggacagcaga gtttcaccga agccgtcttc tactgcgtcc agagcaacgt
    17641 caacttcgtc acctacctgg atttccagct cgccctgggc gtttttgaac tgctcagccg
    17701 ggatagcgga ctcagatttc agaccagcgt caaccagtac tacgtctttg tcgatagcaa
    17761 caacaacgcc acgaacgata gaacccgggc gggtttcgat ttcttttaag gactcttcaa
    17821 agagttgagc aaaagattca gtcatgttta atcttcaggt ttatatttaa cgtccacctg
    17881 gctccatgcc ggatggggtt gtttaacata cccgctgtca ttccattgca acgggggtac
    17941 tgcaaattcg gtcgcttatg cgagagccaa tttctggcgc gcgtattgta gcgctttttc
    18001 aatcaattgc tcaatgctta aggtggtgga atccaacact aaagcatcgg ctgccggaac
    18061 cagtggcgct accgctcggt tacgatcgcg gtcgtcgcgt tctttgatct cggccaaaag
    18121 gcgctcgaag ttaacactaa agcccttctc ctgcaactgt agcatgcggc gatgcgcacg
    18181 ttcttccgag gaggcgtcaa ggaaaatttt cactggtgca tcagggaata ccaccgttcc
    18241 catgtcgcgg ccatcggcaa tcagacctgg taattcgcga aacgcgcgtt ggcgacgcaa
    18301 taatgcttca cgaacgcgtg ggaatgccgc gacttgtgaa gccgcattcg ccacttcctg
    18361 agtacgaatt tcgccgctga catcttcccc ttcgaggatc acttccagat tgccattggt
    18421 cgacacaaaa cgtacatcca gatgggatgc cagcggtacc agcgcatctt ccgacgcaac
    18481 atcaacatgg tgatgtaatg ccgccaatgc cagtacgcga taaattgcac ccgagtccag
    18541 cagatgccat tgcaacgctt ccgccatagc cttacacaag gtgcctttcc ctgcaccgct
    18601 tgggccatca atggtaataa ccggggcaat tgccgtcatc tttatctcct taaaacaggc
    18661 gtaccgttaa cataaccgcg cgcattatac gcgccgacgc cacaaatcgt taacgttgtg
    18721 tgcaaaaacc agttttgtct gtattcagat ggggatttat tgcgcaatta tagcgggctg
    18781 gcagaagacc agcccgaaag atgacaatga tttacttagc cagaggcgat acgatcacgg
    18841 atgtgttgcg cacgctctgt tgatggtggg tgagagtcaa acatggactg ggtgcgaccg
    18901 ccatccaggc tagccagttt ttcaaagctg ccaaccagcc cctgggtgct gatgccacgt
    18961 ttcttcaaca gatcaaaaga gaaatcatct gcttcggact ctttatcacg ggagtattta
    19021 gcattaatag cgccttctgc gatatcaccc aattgtgagc gggaaagctg ggaagccaca
    19081 ccgctggtag ctgaaatggc atcgcgtgcg gcaacgatcg cataagaagc tttcatttca
    19141 gccagcgagt gacccaacgc gacgtggccc agttcatggc ccagaacacc ttcaatttcg
    19201 ttgtcattca tcatgtccat caggccactg tagacacgaa cacagccgtt cgccatcgcc
    19261 catgcgttga cgtcgctggt catataaacc ttatagttga ccggcgtgcc attaatattg
    19321 ttacccaatg ctttggcgat ttttgccaga cgtttaccgt acttgctgga tttacttgcg
    19381 acttgattgc cgctgtccat ttcggcacag ccctgattgg caatcgcttt tgcatcggca
    19441 tcgcttaatg ttgctgcttt ataggcattt aaaccggagc tgatagccat attggtatca
    19501 ataccgtggg tattttgaca ccctgtcagt aatgctgcag aggtcgcaat cgccagcagt
    19561 aatttagtat tcttcatgtg aatcatccgt tgattaatca tatataaata ttatgtatca
    19621 acaacttaca ttgacaagcg acgctaccat taatcaaaat gactcacaag gtccgaaaaa
    19681 aaacgccgac aatgtgc
//
LOCUS       DAJTUN010000078        19695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTUN010000078 DAJTUN010000000
VERSION     DAJTUN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.078"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1285)
                     /gene="gltD"
                     /locus_tag="OO470_002544"
     CDS             complement(<1..1285)
                     /gene="gltD"
                     /locus_tag="OO470_002544"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6251758.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGN"
     gene            complement(1298..5758)
                     /gene="gltB"
                     /locus_tag="OO470_002545"
     CDS             complement(1298..5758)
                     /gene="gltB"
                     /locus_tag="OO470_002545"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6251759.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            6433..7362
                     /locus_tag="OO470_002546"
     CDS             6433..7362
                     /locus_tag="OO470_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6251760.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            7458..9794
                     /gene="arcB"
                     /locus_tag="OO470_002547"
     CDS             7458..9794
                     /gene="arcB"
                     /locus_tag="OO470_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6251761.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10024..10677
                     /gene="elbB"
                     /locus_tag="OO470_002548"
     CDS             10024..10677
                     /gene="elbB"
                     /locus_tag="OO470_002548"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6251762.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            10674..11401
                     /gene="mtgA"
                     /locus_tag="OO470_002549"
                     /pseudo
     CDS             10674..11401
                     /gene="mtgA"
                     /locus_tag="OO470_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(11398..12024)
                     /gene="yrbL"
                     /locus_tag="OO470_002550"
     CDS             complement(11398..12024)
                     /gene="yrbL"
                     /locus_tag="OO470_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6251763.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(12237..12509)
                     /gene="npr"
                     /locus_tag="OO470_002551"
     CDS             complement(12237..12509)
                     /gene="npr"
                     /locus_tag="OO470_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6251764.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(12506..13360)
                     /gene="rapZ"
                     /locus_tag="OO470_002552"
     CDS             complement(12506..13360)
                     /gene="rapZ"
                     /locus_tag="OO470_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6251765.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(13406..13897)
                     /gene="ptsN"
                     /locus_tag="OO470_002553"
     CDS             complement(13406..13897)
                     /gene="ptsN"
                     /locus_tag="OO470_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6251766.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14015..14302)
                     /gene="hpf"
                     /locus_tag="OO470_002554"
     CDS             complement(14015..14302)
                     /gene="hpf"
                     /locus_tag="OO470_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6251767.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(14325..15758)
                     /gene="rpoN"
                     /locus_tag="OO470_002555"
     CDS             complement(14325..15758)
                     /gene="rpoN"
                     /locus_tag="OO470_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6251768.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(15806..16531)
                     /gene="lptB"
                     /locus_tag="OO470_002556"
     CDS             complement(15806..16531)
                     /gene="lptB"
                     /locus_tag="OO470_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6251769.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(16538..17095)
                     /gene="lptA"
                     /locus_tag="OO470_002557"
     CDS             complement(16538..17095)
                     /gene="lptA"
                     /locus_tag="OO470_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6251770.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17064..17639)
                     /gene="lptC"
                     /locus_tag="OO470_002558"
     CDS             complement(17064..17639)
                     /gene="lptC"
                     /locus_tag="OO470_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6251771.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(17636..18202)
                     /gene="kdsC"
                     /locus_tag="OO470_002559"
     CDS             complement(17636..18202)
                     /gene="kdsC"
                     /locus_tag="OO470_002559"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6251772.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(18223..19209)
                     /gene="kdsD"
                     /locus_tag="OO470_002560"
     CDS             complement(18223..19209)
                     /gene="kdsD"
                     /locus_tag="OO470_002560"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6251773.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(19223..>19695)
                     /locus_tag="OO470_002561"
     CDS             complement(19223..>19695)
                     /locus_tag="OO470_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="YrbG; inner membrane protein involved in cell
                     envelope integrity; putative sodium ion/calcium ion
                     exchanger; in E. coli it is non essential for cell
                     viability; member of the YRBG family of cation/Ca2+
                     exchangers; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6251774.1"
                     /translation="GGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMAL
                     TAIAIGTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLA
                     YSRDYSVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
BASE COUNT         4530 a         5113 c         5232 g         4820 t
ORIGIN      
        1 cgttgccttc cggagcgatg atgcggcctt gtgaatccag ctcgacgctg tgttttgcca
       61 gccattccat gttgtgtgga cggaaaccaa acgccatgat caccgcatct gccggaacga
      121 tatgttcgga acctgcaacg atctccgcgc ggcgacggcc tttggcatcc ggttcgccca
      181 tttcggtacg taccattttt acgccgctga ctttgccgtt accgttcact tcaatcccca
      241 gtggctggac gttgaatttg aactctacgc cttcttcccg cgcgtttttc acttcgcggc
      301 gggaacccgg catgttctct tcatcacgac gataggcaca ggtaacgtgc tttgccccct
      361 ggcgtacaga cgtgcgcacg cagtccatcg cagtgtcgcc accgccaagt actaccacgc
      421 gtttgccttc catgctgacg aacggttcgt cgcgggtttc accaaagccc attaactgtt
      481 tggtgttggc gataaggaac ggcagcgctg cgtacacgcc atcggcgtct tcgttttcca
      541 gcccgccgcg cattgactga taagtcccaa cgccaaggaa cacggcatcg taatcactca
      601 gcagatcgtc cagctgtacg tcgcggccca cttcggtatt gagtttgaat tcaataccca
      661 tgcctgtgaa gatttcacgg cgacgcgtca ttacctcttt ttccagcttg aaggccggaa
      721 taccgaaggt cagcagcccg ccaatttctg gatgacggtc gaagacaacg gcttttacgc
      781 cgttacgcgt caggacatcc gcacacgcca gacctgccgg gcctgcaccg ataatcgcca
      841 cttttttacc ggtttgcttc acgccggaca tatccggacg ccagcccatt tcgaacgctt
      901 tatcattgat ataacgctca atgttgccga tggtcaccgc accgaactca tcgttcaggg
      961 tgcaggaacc ttcgcacaga cggtcttgcg ggcagacacg tccgcatact tccggcagtg
     1021 tgttggtctg atgcgacagt tctgccgctt caaaaatacg cccctcgttg gcgagcttca
     1081 gccagtttgg gatgtagttg tgtaccgggc atttccactc gcagtatggg ttgccgcacg
     1141 acagacagcg gtcagcctgc gctttggcct ggccttcgga aaacggctcg taaatttcaa
     1201 caaactcaat tttacggatc ttcagcggtt tctttggcgg atcaacgcgc tgcaggtcga
     1261 taaattgata aacattctga ctcattgttg ctacccctta ctgcgcctgc acgcgcaact
     1321 ctgctgcgct acgactacgg tgacccagca gtgcttttac atcactggac ttcggtttaa
     1381 ccagcgcgaa tttcgtggcg aaggttgacc agttcgccag aatctcttca ccgcgctgag
     1441 aaccggtatg ctgaacatgc tcggtgataa gaccgcgcag atgctcttca tggatcgcca
     1501 gatcgtcgac gcttaagact tcgaccagtt ccgggttaac gcgtttgcgg aaatcaccgc
     1561 tttcatcgag aacgtaagcg aagccgccgg tcatgcccgc gccgaagtta acgccggttt
     1621 tacccagaat acagacaata ccgcccgtca tatattcaca gccgttgtcg ccaatgcctt
     1681 ctaccacggt gatagcaccg gagttccgca cgccgaaacg ttcacccgcg cggcctgcgg
     1741 catacagacg accaccggtc gcgccataca ggcaggtgtt gccgataatg cttgcttcat
     1801 ggctgcggaa ggcggaacca accggaggac gaatggcgat taagccgccc gccatgcctt
     1861 taccgacata gtcgttggca tccccggtca ggtacagttc cacgccgccc gcgttccaca
     1921 caccgaaact ctggcctgcg gtgccgttga agtacgcttt gataggatcg gctgccagcc
     1981 cctgatcgcc gtgcgtctgg gcgatatagc ctgaaagcga cgcgccgaca gaacggtcgg
     2041 tgttgcggat atcgaaccag aaggttttgc tctggcgttc atcgacaaac ggtttcgcct
     2101 gttgcagcaa ctgcgcgttc agtaggccgt tatcaaacgg cgggttgttt tcagtgcagt
     2161 agagtgactt acctgcatgt ggctcggcag tctccagcag cttcgacagc gccagtttct
     2221 gctgtttggc ggtgaaaccg tccagctctt tcagcaggtc ggtgcgacca atcagatcca
     2281 ccagacgcgt tactcccagc tgtgccatca gctcgcgggt ttcacgggcg ataaactcaa
     2341 agtaattagt caccttgaac ggcaggccgt gatagtgatt cttacgcagt ttgtcatcct
     2401 gagttgctac acccgtggcg cagttgttca ggtggcaaat acgcagatat ttacagccga
     2461 gcgccaccat tgggccagtg ccgaagccga agctttctgc gccgagaatc gccgctttga
     2521 tgatatcgac acccgttttc aggccgccat caacctgcaa acgaatttta tgacgcaagc
     2581 cgttggcaac cagcgcctgt tgggtttcca ccagaccgag ttcccacggg cagcctgcat
     2641 atttcactga cgacagcggg cttgcgccgg tgccgccgtc atagcccgcg atggtgatca
     2701 agtccgcata agctttcgcc acgccagtcg cgatggtgcc aacgcctggt tcggacacca
     2761 gcttaacgga gatcatcgct ttcgggttaa cctgcttgag gtcgaaaata agctgcgcta
     2821 agtcttcgat agagtagata tcgtggtgcg gcggcgggga gatcagcgtc actccgggca
     2881 ccgaatagcg cagtttggcg atgtaaggag tgactttgtc gcccggcaac tgaccacctt
     2941 cgcccggctt cgcgccctgg gcgactttaa tctgaatgac gtcggcattg accagatacg
     3001 ccggagtaac gccgaagcga ccagaagcca cctgcttgat gcgcgacact ttgttagtgc
     3061 catagcgcgc cgggtcttcg ccgccttcac cggagttcga gttaccgccg atgctattca
     3121 tcgcttccgc cagcgcctcg tgggcttccg ggcttaacgc gccgatagac atcgcggcgg
     3181 tatcaaagcg tttaaacagt tcgcttgccg gttcaacatc agcaatgttg accgcgtttt
     3241 cacccggcgt aattgccagc agatcgcgca gcgtggttgc cggacgctca ttaaccagct
     3301 tcgcgtattc ctgatagtcg ctgtactcgc cgctttgtac cgcctgttgc agcgtgcgca
     3361 ccacgtccgg gttgtaggcg tggtattcgc cgccgtggac gtatttcagc aggccgccct
     3421 ggctaatcgg cttgcgcgcc agccaggcac gtttcgacag attcagcaga tcctgctgga
     3481 agtcttcaaa gcttgcccca ccaatgcggc tgaccgcgcc ctggaagcac aggcccacta
     3541 catcatcgtg tagaccgacc gcttcaaaca gtttcgagca gcggtaagag gcgatggtgg
     3601 agatgcccat tttggacatg attttgtaca agcctttgtt gatgccgtta cggtagttga
     3661 gcatcacggt acgataatct ttggcaatcg cgtgggtgtc taccaggcgg cccagcgttt
     3721 cataggcaag gtacggataa atagccgtcg cgccgaaacc cagcaacacg gcgaagtggt
     3781 gcggatcgcg ggcgctggcg gtttcgacga tgatgttggc atcgcaacgc aggctttgat
     3841 cgaccagacg ggtctggatc gcgccaaccg ccatcggagc cggaaccggc aggcgatctt
     3901 tagcgatatt ccggtcggag agcaccagca acacggtgcc gctacgtacc attttttcgg
     3961 ctttgtcgca tagctctttg actgtcgctt cgagcgtggt tttagtgacg tcaaaggtga
     4021 tatccagcgt atctgcgcgg tagtgctcct ctttcatcgt cgtgagctgt ttgaaatcgg
     4081 agtagagcag aatcggcgat ttaaagctta aacggtgcgc ctggccctct gcttcgcaaa
     4141 agacgttcat ttcacgaccg atactggtgg cgagcgacat aacatgcgct tcacgcagcg
     4201 ggtcgattgg cgggttagtc acctgggcaa actgctggcg gaagtagtcg taaataatgc
     4261 gcggctgact ggagagcacg gcgaatgggg tatcatcgcc catcgaaccg accgcttcct
     4321 gaccgttttc gcccagtacg cgaattacgg agtccagctc ttccgcgctg tagttaaact
     4381 gtttctggta gctggcaagc gtgtcgtcgt ccagttcgcg gctacccact tcttcatcgg
     4441 gcagatcttc aaacggtacc agtcggcgga cgtttttctc catccactct ttataagggt
     4501 ggcggctttt cagatcgtca tcggtttctg ccgagtgcag aatacgccca ctgcgagtgt
     4561 cgatcaccat cagttcgcct ggcccgacgc ggcctttttc gaccacttcg tcaggctggt
     4621 aatcccagat accgacttca gaggcgcagg tgatgagctt gtctttggta atgacgtagc
     4681 gtgccggacg cagaccgtta cggtcgaggt tacaggcagc gaagcgacca tcggacatca
     4741 cgatgcccgc cggaccatcc cacggctcca tatgcatgga gttaaagtca aagaaggcac
     4801 gcagttccgg gtccatatcc gggttgttct gccaggcggg tggcactaac aggcgcatgg
     4861 cgcggatgat atccatcccg cctgccagca gcagttccag catgttatcc atggaactgg
     4921 agtcagaacc ggtttcgttg acgaatggcg cggcgtcgtg caggtcaggg atcagcggcg
     4981 tctggaattt ataggtacgc gcacgcgccc attggcggtt accggtgatg gtgttgattt
     5041 caccgttatg cgccagatag cggaacggtt gcgccagcgg ccagcgcggt acggtgttag
     5101 tggagaagcg ctggtggaac aggcaaatgg ccgattccag acgcaggtcc gcaagatcca
     5161 gataaaagcg cggcagatcc gccggcatac acagaccttt atagatgttc accagattcg
     5221 acaggctaca gacgtagaag tctttgtcgg cttcgagacg cttttcaatg cggcggcggg
     5281 cgataaacag acggcgctcc atatcgcgtg gacgccagcc tgccggggcg ttcacaaaaa
     5341 tttgctcaat gcgtggcaga gaggagaggg cgatttcacc cagcacgcct tcgttagtgg
     5401 ggacatcacg ccagcccaca atcgacaagg tttcgcgttg cagttcttct tcaacgatgc
     5461 ggcgtgcggc agcggcgagt tcaggatctt tattcaggaa gagcatcccg acagcgtagt
     5521 tttttgctaa acgccagccg cgctcctgcg caacgatgcg aaaaaagcga tccggttttt
     5581 gtaacagcaa gccgcaaccg tcgccggttt taccatcggc gagaatcgcg ccacggtgct
     5641 gcatgcgggc cagtgcgtgt attgcagtac gcactacctt gtggctaggt tcgccttcta
     5701 tgtgggcgat caggccgaaa ccacagttat ccctctcaag ggatttatcg tacaacatat
     5761 cgtgaacctc ccccaggctc tgcgggaacc ccacttccga accgctgcgc acgggcacag
     5821 aaagagcgtg gcgacggggt ttgcgtgtca tacgcgcgcc tcgcattcgc ccccttgttg
     5881 tccttttcgc atcggttaat acggtgaaga ggggatctca attactgcat aaatatgatg
     5941 agcaggctgc tcatccagaa agcttccagc ggatttccaa cttatcggga aagctgatac
     6001 aggtcaaatg gcaagcttat tggtacagaa atgtgcttta gcgcaaaatt ctttattaaa
     6061 tagctgaaat taaaatagat tttatcttta aggctaaaag gagagtcgtt agagaaacag
     6121 tcaattcctg actgtcataa aggcgtgtta acccttatcc tgccttgaac tgacgagaaa
     6181 accagcattt tatactgcct taattggtaa tgacgtttca tgctactgtt tttgcctaaa
     6241 atccataagg agataaaaaa atctgacaca agataagcaa aagtgattta tgtgattgtg
     6301 catgaaattt cggtaatttt gattgattat gcagttgata aaagcaggcc gggacgattg
     6361 acccaggtca tcgccgaatt gagctaaaaa tggcaggctt tggcctcttt tcaaggcgcg
     6421 gtctatcaga ttatgcagtt acagaaatta gtcaatatgt ttggtggtga tctcacccgt
     6481 cgttatgggc aaaaggtgca taagctgacg ctgcacggcg gttttagctg ccctaaccgt
     6541 gacggtacca tcgggcgtgg tggctgcaca ttctgtaatg ttgcctcgtt tgccgatgaa
     6601 gcgcagcagc atcgttccat tgccgagcaa ctggcgcacc aggcgaattt agttaaccgc
     6661 gctaaacgct atctggccta ctttcaggcg tataccagca cctttgcgga agttcaggtg
     6721 ctgcgttcta tgtatcagca ggcggtgagc caggccaata ttgtcggttt gtgtgttggt
     6781 acccgcccgg actgcgtgcc ggatgcggtg ctggatctgc tttgcgaata taaggaccag
     6841 ggttacgaag tgtggctgga gctggggcta caaaccgccc acgacaaaac actgcatcgc
     6901 atcaaccgcg gccatgattt tgcctgttat cagcgtacaa cccagctggc acgtcagcgc
     6961 gggctgaagg tgtgttccca cttaattgtc ggcctgcctg gcgaagggca ggctgaatgt
     7021 ttgcaaacgc tggagcgagt ggttgaaacc agcgtggacg gcataaagct gcatccgctg
     7081 catattgtga aaggcagcat tatggcgaaa gcctgggaag cggtgcgttt gaacggtatt
     7141 gaactggagg attatacgct cactgccgga gaaatgattc gccatacgcc accggaagtg
     7201 atctaccacc gcatttccgc cagcgcccgt cgcccgacgc tgcttgctcc gctgtggtgc
     7261 gaaaatcgat ggacgggaat ggtcgagctg gatcgctatc tgaatgaaca tggcgtacag
     7321 ggatcggcgc tgggacgtcc gtggctacct ccaacggagt aggtcgttga ggggaattcc
     7381 gcatttctca cacaatttat aacgtaactg tcagaattgg gtattattgg ggcaggttgt
     7441 cgtgaaggaa ttccctaatg aagcaaattc gtctgctggc gcagtattat gttgacctga
     7501 tgatgaagtt aggtctggtg cgcttctcaa tgttgctggc gctggccctc gtcgttcttg
     7561 ccattgtggt acaaatggcg gtaaccatgg tgctgcatgg tcaggtcgaa agcattgatg
     7621 ttattcgttc tatcttcttt ggtttgctga ttacgccgtg ggcggtctac tttctatcgg
     7681 tggtcgtcga gcaactggag gagtcacgac aacgtctgtc acggctggtg caaaaactgg
     7741 aggagatgcg cgagcgcgat ttgagcctca acgttcagtt aaaagataat attgcccagc
     7801 taaatcagga aattgccgtt cgtgaaaaag cggaagcaga actgcaggaa accttcggcc
     7861 aactgaaaat tgaaatcaaa gagcgcgaag agacacaaat tcagctcgag cagcaatcct
     7921 cattcttacg ttccttcctt gatgcttcac ccgacttggt tttttatcgt aacgaagata
     7981 aagagttttc cggctgtaac cgcgcgatgg agctgctgac cggaaaaagc gaaaaacaac
     8041 tggttcacct gaaacctgct gatgtttact caccggaagc cgccgcaaaa gtcattgaaa
     8101 ccgatgaaaa agtgttccgt cataatgtgt cactgaccta tgaacagtgg ctggattacc
     8161 cggacgggcg caaagcctgc tttgaaatcc gtaaagtgcc gtactacgac cgcgtgggta
     8221 aacgtcacgg tttgatgggc tttggtcgcg acattaccga gcgtaagcgg tatcaggatg
     8281 cgcttgaacg cgccagccgc gacaaaacga cgtttatttc caccatcagt cacgaattgc
     8341 gtacaccgct gaacggtatc gtcggcctga gccgcattct gctggatacc gaactcaccg
     8401 ccgagcagga aaaatatctc aaaactatcc atgtttcggc cgtcacgctg gggaatatct
     8461 ttaacgatat tatcgacatg gataagatgg aacggcgcaa ggtccagctt gataatcagc
     8521 cggttgattt caccagcttc cttgccgatc tggaaaatct ctccgcattg caggcgcaac
     8581 aaaaaggatt gcgctttaac ctggagccta cgctgccatt accgcatcag gtcattaccg
     8641 acgggacgcg tttacggcag atcctgtgga acctcatcag taacgccgtc aaattcaccc
     8701 agcaaggcca ggttaccgtg cgcgtgcgct acgatgaagg cgatatgctg cattttgaag
     8761 tggaagattc tggtatcggc attccgcagg atgagctgga taaaatcttc gccatgtatt
     8821 accaggtgaa agacagtcat ggcggtaaac ctgccaccgg caccggtatt ggtctggccg
     8881 tttctcgtcg tctggcgaaa aatatgggcg gcgatattac ggttaccagt gaacagggca
     8941 aaggttcaac ctttacgttg acgatccacg caccgtcggt ggcagaagag gtcgatgatg
     9001 cgtttgatga agacgatatg cctttaccgg cgctgaatgt gctgctggtg gaagacattg
     9061 aactgaacgt gattgtcgcg cgttctgtgc tggaaaaatt aggtaacagc gttgatgtcg
     9121 ccatgaccgg caaggcggcg ctggagatgt ttaaaccggg cgaatacgac ctggtgttgc
     9181 tggatattca gttgccagat atgaccgggc tggatatctc tcgtgaactg acgaagcgtt
     9241 atccgcgcga ggatttaccg ccgctggtgg ccttaaccgc taacgtgctg aaagacaaac
     9301 aagagtacct caatgctgga atggatgatg tgctgagtaa gccgctttcg gttccggcgc
     9361 taaccgcgat gatcaagaaa ttctgggata cccaggatga tgaggagagt acggtgacga
     9421 cagaagagaa cagtaaatca gaagcattgc tcgatattcc catgctggaa cagtatctcg
     9481 aacttgtagg accgaagctg atcaccgacg ggttagcggt gtttgagaag atgatgccgg
     9541 gctatgtcag cgtgctggag ttgaatctga cggcgcagga taaaaaaggc attgttgagg
     9601 aaggacataa aattaaaggt gcggcggggt cagtggggtt acgccatctg caacagctgg
     9661 gtcagcaaat tcagtctcct gaccttcccg cctgggaaga taacgtcggt gaatggattg
     9721 aagagatgaa agaagagtgg cgtcacgacg tagaagtgct gaaagcgtgg gtggcaaaag
     9781 ccactaaaaa atgaccccgg ctagaccggg gtgcgcgaat actgcgccaa caccagggaa
     9841 atcttggctg cgccgtaaat tattatgatg agttacaagg gcacagcact gtttttcagg
     9901 ccgcacgcag ttaagatagc aaaacttaaa tgatttgtta catgaatcag ttaaatgtgt
     9961 gatgcgtacc atttttatta aaaaaattaa tgtgtttcag tcaattgcga aaggatgatc
    10021 acaatgaaga aaattggcgt aattctgagc ggatgcggcg tctatgacgg ttctgaaatt
    10081 catgaagcgg tgttgacgtt gttagctatt tcacgcagcg gtgcgcaggc ggtctgcttt
    10141 gcaccggata agcagcaggt tgatgttatc aaccatttaa ctggcgaagc gatgacggaa
    10201 acgcgcaatg tgctgattga agcggcacga ataacgcgcg gtgaaatccg tcctctggcc
    10261 caggccgatg ccgctgaact ggatgcgttg attgtgccgg ggggggttgg cgcggcgaag
    10321 aatttaagca attttgccag tcttggtagc gaatgcaccg ttgaccgtga attaaaggcg
    10381 ctggcacaag cgatgcatca ggccggaaaa ccgcttggtt ttatgtgtat tgccccggcg
    10441 atgctgccga aaattttcga tttcccgctg cgtttgacca tcggtactga tatcgatacc
    10501 gcagaagtgc tggaagagat gggcgcggag catgtgccgt gtcctgtcga tgatatcgtg
    10561 gttgatgaag acaataagat tgtcaccacc ccagcatata tgctggcgca gaacattgca
    10621 gaagcggcga gcggcattga taagctggtt tcccgcgtgc tggttctggc tgaatgagta
    10681 aaagccgctt aacggtgttt agtttcgttc gccgttttct tttgcggtta atggttgtcc
    10741 tcgccgtttt ctggggcggg ggcatcgcgt tgtttagcgt tgcgcctgtt cccttctcag
    10801 cggtaatggt cgagcgacag gtcagcgcct ggctgcatgg caattttcgt tacgtggcac
    10861 attctgactg ggtcagtatg gatcaaatct cgccgtggat gggactggcg gtgattgccg
    10921 cagaagatca gaaatttcct gagcactggg gctttgatgt cgcttccatt gagaaagccc
    10981 tggcgcacaa cgagcgcaat gaaaaccgta ttcgcggtgc ttcaacgatt tctcagcaga
    11041 cggccaaaat ctctttttat gggatgggcg tagctgggta cgaaaagggc tggaagctgg
    11101 attaacgctg gggatagaaa cagtctggag caaaaagcgt atcctgacgg tttacctgaa
    11161 tatcgccgaa tttggcgacg gtgtgtttgg cgtcgaagct gcggcacaac gttatttcca
    11221 caaacccgcg agcaaactta cccggtcgga agctgcatta ctggcagctg tattacctaa
    11281 tccacttcgt ttcaaagtct cctcgccatc gggctacgtg cgtagccgtc aggcgtggat
    11341 tttacggcag atgtatcagt taggcggtga gccgtttatg caacaacacc agctggatta
    11401 agcttcgcgc gaagtgaggg ccagcgtttt gctctcacga ggttgcagat ctttctgcag
    11461 ggcaatagcc agcgccggtt gggcgataaa tcgtttccat aaccgctctt gcttacgcag
    11521 acaacaccac ttcgaccagg tcgccagtgg aatcaatgtg ctttcaccaa tgttgtcgca
    11581 gacgaccggg ataacctctg actcactgat gcggtgacag aggatattct gcggcttcag
    11641 cgacatcgtc acgatacggt tatcctgcaa ataacgcttc agctgtttca gtagctggcg
    11701 cagttgtgca atgtcttctt cgtaacggca ttgttcagca aattcggtca gcgtaatgga
    11761 aggcttgccg tcaaaatcgg caatcacatc gtagacataa ccggttccgc aatccgtctc
    11821 aacggtaccg tgatagcgcg gtattccact ccagtctttt aaccggcgac caagatgcgc
    11881 gtagtacttt aactcacggc ggatctcttt atcgccacca cgatggtaga caatcttgat
    11941 acagcgttgg gcatcttccg gatgcgcata acatttccga tgtcttccgg tgcccagggg
    12001 actttgttca gataagcgaa tcatgccatc tcctggaaat attttctgac gatctccagt
    12061 atggtcgatt aacttaaaca aaacctaaac aatgcctctt aaggttttct taaggttctt
    12121 ctgaaagtga aaagcgcaca aaatgtacaa atgacgttga ttgagaacga tttcagtacg
    12181 tgattttctt attgtcgggg gagcttgaag ggagttgtat gtcaaagtga tgaagattaa
    12241 tcttcatcaa aaccagaatt aaagagggcg ataacggcgg ccagtgcttc ctcttcctgt
    12301 ggaccggtcg cttcaacttc aatctgccgt cctttggcag aatccaacat cagcagcgca
    12361 ataacgctgt tggcttcagc ctcggtgcct tcgtcattac gtaacagcac ttcagcgtca
    12421 aaaccctgca ttaattcaaa cagcttcatt gcaggccggg catgcatgcc cagcttgttt
    12481 gtgatttcaa cagtttgctt gacggtcatg gtttacgttt ttccagcgta cgatggcgtg
    12541 actggacgtt tttaccgcgc gagcggaagt agtctgccag ttgctctgca atatacaccg
    12601 aacggtgctt cccgccggta caaccaatgg cgaccgtcag gtagctacgg ttgttggttt
    12661 ccagcatagg taaccatagc tcaagatagc ttcgcgtctg gtagataaaa ttgtgtactt
    12721 ctgtgtggcg gtcgaggaac gcggcgacag gtttatcaag acctgtcatt ggacgcagtt
    12781 tcggatccca gtgcgggttc ggcaagaagc gcacgtcaaa gacgtaatct gcatcgatag
    12841 ggataccgtg tttgaagccg aaagactcaa agaccatggt cagttcgcgt tcacgtttac
    12901 ccagcagacg ggtacgcagc atttcagcca gctcgtgaac ggacatttct gaggtatcga
    12961 caatcagatc cgctcgcgaa cgcagaggct ccagcaaatc gctttctttg tcgatagcac
    13021 tttccagcga caggtttttg ctggaaagcg gatgcagtcg gcgcgtgtca ctgtaacgac
    13081 gaattaaggt attacggtcg gcatccagga acagtagttg cggtgagaaa gcgtcaggca
    13141 ggttactcat cgcctgttcg aatatttctg gtgactccgg catattacga acatcaatgc
    13201 tgacggcggc agaaatctcg cgatcggcca gagttcgagc cagatcgggt aacaacacta
    13261 cgggaaggtt atccacgcag taaaaaccca tatcttccag cgcacgcagg gcgacagatt
    13321 tacctgaacc tgaacgtccg ctgacgatca tcagtaccat gtactgtttc tcctcacaac
    13381 gtctaaaaga gacattaccg aataactacg cttcatccgg agtaccttcg gtatccgtaa
    13441 tgatttgata cagctcttca tcgctctggg ctgcgcgtaa acggcggcag atggttttgt
    13501 ccgccagacg tttcgccacc agcgacagag tatgcaggtg cgttttagtt tggtctgccg
    13561 gcaccagcag ggcaaaaagt aagtccaccg gttggttgtc gatggcatcg aaagctatag
    13621 gcgtttcgag ctgaacgaaa acgccaacgg cacgcagagt atcttcttca agtttgccat
    13681 gcggaatggc aataccattg ccgataccgg tactgcccat tttttcccgc gtcaggatag
    13741 cttcaaaaac cacctgaggc ggcaggctaa gttgtttcgc cgccagctcg ctgatgattt
    13801 ccagggcgcg ttttttgctc tgacagtgga cgcggcttcg cgtacattcc ctgttaagaa
    13861 cactgctaag ctgtagagtt gtatcattat ttgtcataat ttcacctaag aacctgccca
    13921 ctcaaacagt ggaacaggct cggagcatta accgtacaaa tggcccgttg tgccgcacaa
    13981 caggccgcca tgcacatgct aattgcccgg acaattagtg ttgtttcagt ttatctttat
    14041 gtttggtcaa ctggcgtgcc agcttatcaa ttaagccatc aatggcagcg tacatgtcct
    14101 gaccttccgc gctggcatga atttcaccac cgtttacatg cagtgttgca tctgaggtgt
    14161 gggtgacttt ctccactttc agaacaacat agacctggtt gattcggtca aaatattgct
    14221 caagtttggc aaatttagct gtaacaaatt cgcgcagtgc ctcggtgatc tcgacgttat
    14281 ttccggtaat gttgagctgc atagtgtctt ccttatcggt tgggtcaaac gagctgttta
    14341 cgctggtttg acggcggaat ggataaagac tctcggtact tcgcaacagt gcggcgtgcc
    14401 accatgatac cttgttccga cagcaaagag gttaacttgc tgtcgctcaa cggtttcgct
    14461 gggttttccg ccgcgattaa tttcttcacc agtgcacgaa tcgccgtgga ggaagcttcg
    14521 ccgccgccct cggtattgac gtgactggaa aagaaatact tcagttcaaa aatgcctcgt
    14581 ggactatgca ggtatttttg cgtggtcacg cgagatatcg tcgattcatg catttcgaca
    14641 gcctgggcga tatcggccag taccatcggt ttcatatatt cttcaccttg ctcaaagaag
    14701 gcttgctgct gttcaacgat acagcgactc acgcgcagta gcgtatcgtt acggctttcc
    14761 agactcttga tcaaccattt ggcatcctgc agattgctgc ggataaactg gctgtcacca
    14821 tcgttgcgcg cgttattgca catcgaggcg tagtgctggt tgatttgcag acgcggaatg
    14881 ctgtcactgt tgagttctac cgtccagtga ccgttatgct tacgcaccag cacatctgga
    14941 atgacatact caggttcgcc agtctggatc gactgcccgg ggcgcggatc gagcgactgg
    15001 atcagattga cggcttcttt cagcacatct tctttcagac gcgtgacgcg cattaaagtg
    15061 cggaagtcgt gattggctaa cagatcgaga tgatcgctaa tgatcaatct ggcctcttcc
    15121 agccacggcg tggtcttatc gaattgggag agttggatta gcaggcagtc acgcagatct
    15181 tttgccgcta caccgaccgg gtcaaaccgt tggatccgct taaggacggc ttcaacctcg
    15241 tcgatgtcga tctcttcatc gcccatactt tcgagaatat cttccagcgg gacagtcaga
    15301 taaccggttt catcaacggc atcgacgata gaggtagcaa tagcgcggtc agtgtcggaa
    15361 aacggtgtca gctcgacctg ccacatcagg taatcctgca aggtctgcgt cgtttcgccc
    15421 tggtagaccg gcagctcgtc gtcaatgtag tcaccgctgg tgccggatgg tgtaccagcg
    15481 gtgtaaatgg tgtcccaact ggcatcgagc ggcagctctt ccggcatctc tttttgttcg
    15541 agcgcgtcgg cggtgtccag cgtttcactg tcttgcgttt cgcgggtgtc gatttcttca
    15601 tgagtgtcga tttgctcaag cagcggatta ctctccagcg cctgctgtag ctcctgctga
    15661 agttccagcg tcgacaactg caacagacga attgcctgtt ggagctgtgg cgtcatcgcc
    15721 agttgttggc taagcctgag ttgcaaacct tgcttcatgt tcagaatcgt actctcctgc
    15781 taaaacgtcg caaacttcta ccctatcaga gtctgaagtc ttccccaagg tatacacgct
    15841 taacgtgttc gtcttgtaag atttctgtag gcgtgccgtg ggagatcaaa tgcccctgac
    15901 tgacgatata agcgcgttca caaaccgcca gtgtttcacg cacgttgtgg tcagtgatca
    15961 gcacgcccag gccgctgtcg cgcaggtgtt caatgatgcg tttaatgtcg ataaccgaga
    16021 tcgggtcaac cccggcaaac ggttcgtcga gcagaataaa tttcggattc gcagccagtg
    16081 cgcgggcaat ttctacacgg cgacgttcac ccccggagag tgactgcccc atgctgtcac
    16141 gcaggtgctc aatgtgaaac tcttccatca gctcgttcgc gcggtcttca cgttgttcag
    16201 cagacaagtc gtcacgaatt tgcagtaccg ccatcaggtt atcgtaaacg ctgaggcgac
    16261 ggaaaatgga ggcttcctgt ggcagatagc cgataccgcg gcgcgcgcgt gcatgcagag
    16321 gcagcagact gatatcgtca tcatcaataa tgatgttgcc cgcatcgcgc ggcacaatgc
    16381 ctacaaccat gtagaaagtg gtggtcttac cggcaccgtt tggccccagc agaccgacaa
    16441 tttccccgga gttgacggtc aggctgacgt cttctaccac gcgacggcct ttataggctt
    16501 ttgcaaggtt ctttgcagtt aatgttgcca taacgaatta attacccttc ttctgtgccg
    16561 gggtctggcc tttgttgttt ttgtcctgca gctgcgacgg caccagaacg gtggttacgc
    16621 gcttgccttt gtcgctgaaa gcctgcattt tctgctcttt caccaggtaa gtgatcttat
    16681 cgcccttaat gttgctatcg acctgctgca gataagcatt acccgtcaga acgacaaaat
    16741 cttttgccag ttcgtagtgc atctgggaag cgtgaccttc aacgggttta ccgttgtcct
    16801 gcatctggta gaacgttgcc ggtttaccgt atccgtcaat cacttcttta ccttgttcgc
    16861 cgcccggacg ggtaacgacc actttgtcgg cattaatttt gatggtgccc tgggtgacga
    16921 tgacattacc ggtaaaggta accacgttgc cttgcatatc aagagattgc tggtccgatt
    16981 caatgtggat cggctgatca gtgtctccgg ttacggcaaa tgccggaatg ctggcggcca
    17041 gaagtgagct ggcaagcaca agattaaggc tgagtttgtt tgttttgaat ttcataggat
    17101 gttctaacct tttcaatcag ctcggcgttc ttgctgcgta agttgccgcg cattttcaga
    17161 ccgctggagt taaatgttgt tccgtataac gtgacgaggt cttcagaggt aacatcctgc
    17221 gtcaccagat tgatctgcgc gttatccgtc gtgattctgc gaagttgaga gtctggcaca
    17281 agtgcgttga cttcaacgtg tccatataaa tagagcatcc ggtcattggt cagcttggct
    17341 ttatctgctt ttacggacca tgtcgggatt ttatccttat caaacgtggt aagtaccggc
    17401 tgcgtaaacc acgaaacggc ctgatcggaa taatattcaa cgtgttgagc aatcaatcga
    17461 tagcttagtg ccccttctgg gttatagacg agcgtgtccg tatgctcgct tttataggtg
    17521 ggatcattgt tgttgacgac cacctgggcg gtatcgtctt tttcggccat attaatgccg
    17581 atcatcacca gaaccgccag tgatagcaca atgataaccc aacgtctggc tttactcata
    17641 tcgattgccc tttggcttca tccagtttgc cctgcgccag gagtaataag tcgcaaactt
    17701 cgcgcactgc gccacgaccg ccagcaatgc gcgtcacgta atcggcgcgc gggatcaaca
    17761 gtggatgcgc atcggccacg gcgacgctta aacccacttt ttccattacc ggccagtcga
    17821 tgagatcatc gccaacataa gccacatttt ccggggcaat cgccaatttt tccagcaaat
    17881 cgctaaaggc gatcagtttg tttgactgcc cctgatacaa gtgagtgatc cccaatgtgg
    17941 cacaacgatc ttctaccagt ttagcctttc gcccggtaat gatagcgact tcaatatcag
    18001 aggtgagcgc acaacgaatg ccataaccgt cacgaacatt gaacgctttc agctcttcgc
    18061 cgttattgcc catataaatc aggccatctg acagtacgcc atcaacgtcg aggatcagca
    18121 gacgaatgtt ctctgctttt gccataacgt cggcgctgac agggccgtaa caggtcgcaa
    18181 gcgacgcacc tgctttgctc attgttgttt atccttgaat ctttacacta cgcctgcacg
    18241 cagtaaatca tgcatatgta acacaccgag taaatggtcg ccatcggcaa ccatcacgga
    18301 ggtgatatgg cgggactgca ttaagttcag tgcctcaacg gccagaatgc cagggcgcac
    18361 acgtattccc cccggcgtca tcacatcggc aatacttaac tgacgaacat ccacgcccat
    18421 atcgaagaca cggcgtaaat caccgtcggt aaagatgcct tcaatcatca tattgtcatc
    18481 gcaaatgaca gtcataccaa gatttttgcg ggtaacttcc agcaatgcgt cacgcagact
    18541 ggccgttttc ttaacatgcg ggatctcatc gcccgtatgc ataatatcgt ttacgcgcag
    18601 cagaagttta cgacccagtg cgccgcctgg gtgtgagagc gcaaaatctt cagcagtaaa
    18661 gccgcgtgct tttaacagcg cgacagcgag ggcatcgccc ataaccagcg tggcggtggt
    18721 gctgctggtc ggtgccagcc ctaacggaca ggcttctttc gctactttaa cacacagatg
    18781 cacatctgcg gcgcgcgcca tgctgctctc cgggcgaccg gtgatgcaga ttaacggtac
    18841 gtgaagacgc ttaagcactg gaattaaggc cgtgatttcg ctggattcac ctgagttaga
    18901 gatagcaatc accacatcct gtggggtgac catgcctaaa tcaccatgcg cggcttcact
    18961 aggatggacg aaaaatgaag gtgtaccggt gctggcaaac gttgcggcca ttttgcgccc
    19021 gatgtgcccc gatttcccca tccccatgac gacaactttc cctttacacc agaacatctt
    19081 ttcacaggca agcgtgaaat tctgattgat gtattgatca agctccgcca ggcattcacg
    19141 ttcaatcgcc aggacttctt tacctgcttg ctgaaagtca aaacccggtt gtaactctac
    19201 gtgcgacata atgcgtttcc agttattcaa cgagtattgg cgataaccag tacaacatcg
    19261 ccagccatac gataaatccg ccagttaata atacccctac accacggccc ggttgcgggg
    19321 agcgccgcca gcacagcaac gcaaaaataa tgctcaccag caacatcacg ctgtagtcac
    19381 gactgtacgc cagtggatca atctctcctg gcgttatcag cgcgggtaaa cccaacacga
    19441 tgacaatatt aaaaatgttt gcgccaatga tatttccgac agcaatgtcg ttttcacctt
    19501 tgcgaacccc cgctattgcg gttgccagtt ccggcagact ggttccgata gcaattgccg
    19561 tcagagccat cgtcaactcg ctgatggcaa agtaattcgc cagcaccgtg gcgttatcaa
    19621 ccaccatccg cgtggccact ggcatgatga taagcgcaat gccgagccat aaaaacgcga
    19681 cgggcaatcc gccgt
//
LOCUS       DAJTUN010000079        19524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTUN010000079 DAJTUN010000000
VERSION     DAJTUN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.079"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            258..1088
                     /locus_tag="OO470_002562"
     CDS             258..1088
                     /locus_tag="OO470_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6251775.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1229..2002)
                     /gene="uxuR"
                     /locus_tag="OO470_002563"
     CDS             complement(1229..2002)
                     /gene="uxuR"
                     /locus_tag="OO470_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6251776.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2217..3677)
                     /locus_tag="OO470_002564"
     CDS             complement(2217..3677)
                     /locus_tag="OO470_002564"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6251777.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3758..4942)
                     /gene="uxuA"
                     /locus_tag="OO470_002565"
     CDS             complement(3758..4942)
                     /gene="uxuA"
                     /locus_tag="OO470_002565"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6251778.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5282..6625
                     /gene="gntP"
                     /locus_tag="OO470_002566"
     CDS             5282..6625
                     /gene="gntP"
                     /locus_tag="OO470_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6251779.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6876..7778)
                     /gene="fimH"
                     /locus_tag="OO470_002567"
     CDS             complement(6876..7778)
                     /gene="fimH"
                     /locus_tag="OO470_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6251780.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7798..8301)
                     /gene="fimG"
                     /locus_tag="OO470_002568"
     CDS             complement(7798..8301)
                     /gene="fimG"
                     /locus_tag="OO470_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6251781.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8314..8844)
                     /gene="fimF"
                     /locus_tag="OO470_002569"
     CDS             complement(8314..8844)
                     /gene="fimF"
                     /locus_tag="OO470_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6251782.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8854..11490)
                     /gene="fimD"
                     /locus_tag="OO470_002570"
     CDS             complement(8854..11490)
                     /gene="fimD"
                     /locus_tag="OO470_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6251783.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11557..12282)
                     /gene="fimC"
                     /locus_tag="OO470_002571"
                     /pseudo
     CDS             complement(11557..12282)
                     /gene="fimC"
                     /locus_tag="OO470_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12319..12858)
                     /locus_tag="OO470_002572"
     CDS             complement(12319..12858)
                     /locus_tag="OO470_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6251784.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12923..13471)
                     /gene="fimA"
                     /locus_tag="OO470_002573"
     CDS             complement(12923..13471)
                     /gene="fimA"
                     /locus_tag="OO470_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6251785.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(13951..14547)
                     /gene="fimE"
                     /locus_tag="OO470_002574"
     CDS             complement(13951..14547)
                     /gene="fimE"
                     /locus_tag="OO470_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6251786.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15025..15627)
                     /gene="fimB"
                     /locus_tag="OO470_002575"
                     /pseudo
     CDS             complement(15025..15627)
                     /gene="fimB"
                     /locus_tag="OO470_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17081..17797
                     /gene="nanC"
                     /locus_tag="OO470_002576"
     CDS             17081..17797
                     /gene="nanC"
                     /locus_tag="OO470_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6251787.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17817..18923
                     /gene="nanM"
                     /locus_tag="OO470_002577"
     CDS             17817..18923
                     /gene="nanM"
                     /locus_tag="OO470_002577"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6251788.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            18988..>19524
                     /locus_tag="OO470_002578"
     CDS             18988..>19524
                     /locus_tag="OO470_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6251789.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQ"
BASE COUNT         5166 a         4689 c         4450 g         5219 t
ORIGIN      
        1 gcattattaa tatagacctc aatatagcaa tatcaaattc caattagtaa taagatatgc
       61 ttttaaattg catgcaaata attttaaatg agcgtttttt gatactttag aatgtttcct
      121 accctttaat atcactgtgt agagttgata tcactacaac agaaacatgc atgtcatttg
      181 tttacttctc tttttactac agaaaaattt atctattttt ctaaacatca gttaaatact
      241 cataaaaggt aactattatg tctatgccat taagcaatgc cgctaataac caattcaata
      301 caaataatca cttccttcat catccaaaag tcgacagtga gttaacacgt cgatatgaat
      361 atgcacgcct ggatacggaa aatatctatc ttctgcctct tgctcgtggc aataaccata
      421 attacgatgg aaaaagcgtt gtcgaaatta gaaaacttaa tattggcaaa cagtcctggc
      481 catttaatta tgtgacagat acatgcagag aattcaatgg tattacaaca acaggacgta
      541 tgctctatcg aaacttgaaa atcacctcag ctttagacga aatatatggt ggtatatgta
      601 aaaaggcgca tgctgctaca gagttagcag aaggtttacg gctaaattta tttatgaaga
      661 gccctttcga tcctgttgag gactatacag ttcatgaaat aacattaggc cctggttgca
      721 atgttcctgg ttatgccgga accacgatag gatatatttc aacccttcca gcttctcagg
      781 caaaaagatg gaccaatgag cagccaagaa ttgatatcta tattgatcag ataataactg
      841 tctcaggggt agcaaattct tccggattcg cccttgccgc attacttaat gctaatattg
      901 agatgggaaa tgacccaatc attggtatag aagcctatcc tggtaccgcg gagatccatt
      961 caaaaatggg atataacgtt atccccggtg atgaagatgc gccactcaaa cgcatgactc
     1021 tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa tggtgaatgg aattatattg
     1081 gtaaatagcc actgagaaga atgagtacta atattcaggt tatacagata tttaaacaaa
     1141 accctgcaat ttacaaagta atactcattg cgacttctta atttaagaaa gcgcaaggaa
     1201 cgtttaccct tgcgcttatt ataataagtc aggcgtcgac tttatccagc ggccatgaat
     1261 caaagagata gccgtcaaaa taaatatcgt caacgttcga gaattccagc agacgttgct
     1321 taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct tttttgatta
     1381 acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg ctgtcatcca
     1441 gatggctgtg caactggatc cacattggat tgttttcccg ccactgccag gactgacgga
     1501 ataactccac cagcatgctg ttatgcgttg cttcagcaat ggcgagatgg aactgcatgt
     1561 caccgctttc gctgctaccc ggcgcgctgg aagccagttc acgctcttcc agttgcaatg
     1621 cctgacgcat tttgacgata tcttcgcggg tagcctgcag tgcggcaaac tcggcgatgt
     1681 tactttccag taactgacgc gcctgtaaca gctcaaaagg acccgcatca ttgcagacgt
     1741 tggcatccgg actgtctgtg ttgtggctgc ctgagctatc aagcacatag ataccggcac
     1801 cccggcgcac ttccaccagc cctttgatct ccagcatgat cagcgcttca cgtaccaccg
     1861 tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt tcgccaggat
     1921 tgtacggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg taaggtcttt
     1981 gcgcagaggt gacagatttc atggtgttgt gtccgttagg tgcggttaat caaatttttg
     2041 ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg cccccgttaa
     2101 tctcaaacta taccattagc actggaagtt ggcacgtaca ggtgggcaaa aaaacgaaaa
     2161 ctggtcaacc aaatatcagt ttgattgacc agtaagtctg tagggtaatc agttagttac
     2221 agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc cgccgtcacc
     2281 gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaataccgct caggcccagc
     2341 agcgctttca cgcggtctgc gccctgatac tgcgcgttaa tcttctggaa ctcggccagc
     2401 atcgggtcga ccacgtcaat ggcattaccc tgctcatcca cgccctgggt gtaacccatc
     2461 cagccagcca cgcccagcgc caggtgacgc cagctgccgc cgttttgcag gtgcagacgc
     2521 accgggtcca gtagacgctg cggtaacttc tggctgccgt ccatcgcaat ctgccaggta
     2581 cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata ggcgttcagg
     2641 tccgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcag ggcaaaggcc
     2701 gctttgcgat aatccgggtt agtcatggtg tcggcaatgg tttcatagcc gccgaggtaa
     2761 ccgaggtacg ccaggaaaga gtggctgccg ttcagcatac gcagcttcat catttcgaac
     2821 ggcacaacgt ctgcaacgaa ctgtgtgccc actttatccc agtccgggcg accattaacg
     2881 aagttatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca cgggtcgtaa
     2941 acacccagct ggtcagcaat ttcctgtaag gtttctggcg tcgccgccgg aacgatgcgg
     3001 tcaaccatgg tgcacgggaa ggtgacgttc tcttcaatcc atgccgctag ctgcgggtca
     3061 cgcgcctgag ccagccccag taccgcgacc ttcgccacat ggccgttttc acgcacgtta
     3121 tcgcaggaca tcaccgtaaa tgctttcagc cctttttcac gacgcagacg caaggcttcg
     3181 acgatgtaac caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg cttgatcagc
     3241 gggttattga gatccagttg accgcttgcc gcatcagcgc agtagccttt ttccgtgacc
     3301 gttagagaga caatcgccgt ttgcggacgc gccatcgcgt tgagaatacc ttcgcagccg
     3361 tcgatttccg gatgcagcgc ttctttcatc gaaccgataa ttttcagctc ggtgctttct
     3421 gcgccttttt ccgctacggt gtacagcagt tgctgttttt tcaggttttc gatcagcatg
     3481 cggtcgttgc ctggcatcag gttaacttcg cagatgcccc agtcgctgtc ggtgctttcc
     3541 agcagatgat gggtatacag cgcctggtgc gcgcggtgaa acgccccgca accgagatgc
     3601 acaatgcgtg attccagacg agaatgatcc catgacgggc gggcaaccgg cagattgctg
     3661 tcaacaatag tagtcatcgt aaactccaga atccgtcttc agagagtgac ggcaaggaag
     3721 aaggaaccgg cacggcgcgt agccatgccg gtgggtatta acggctaaag aaagcgcgct
     3781 ggatcgccag ttcgacaccg cgaacttcgg ccaggccttt cagacgacca attgcggagt
     3841 aaccagggtt ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca
     3901 tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcttcttca acaatcgctt
     3961 tcaccacttc gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg
     4021 ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga ccgaactgct
     4081 tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaaccggtg cacatggtga
     4141 aaccgttcgc catgctgttt acggtatcaa ccatccactg catatcttca atggtggaaa
     4201 caatgcgcgg caagccgagg atcgggcgcg gcggatcgtc cgggtgaacc gccatacgca
     4261 cgccaacttc ttcagcaact ggaataatcg ctttcaggaa gacggcaaag ttttcgcgca
     4321 gtttggcttt gtcgatatct ttgtacagct ccaggtgctt acggaactgg tcgagggtat
     4381 acccttcttc cgcgcccggc agaccggcaa tgatgttacg cgtcagacgc gctttgtctt
     4441 cgtcgctcat agtggcgaag cgttcagctg cctgagcaat ttcttcttcg gtgtaatccg
     4501 cttccgcgcc tggacgtttc aggatatgca tttcgaatgc agcgaattcg atctggtcga
     4561 agcgcagagc tttggagccg tctggcagca cgtattcgag gtcagtacgg gtccagtcga
     4621 gcaccggcat gaagttgtag cacacggtgc gaatgccaca ctgcgccagg ttgcgcagag
     4681 tctgctgata gttagcgatc cactgctcat agttgccagt gtgggttttg atatcttcgt
     4741 gaattggtac gctttctacg acagaccaca ccaggcctgc gtcttcaacg atcgccttgc
     4801 gtttgaggat ctcttctacg gaccatactt cgccgttcgg gatatggtgc agcgcggtaa
     4861 ccacgccagt tgcgcccgcc tgacggacat cagctaaaga aaccggatcg tttgggccgt
     4921 accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc cacagcaaag
     4981 agacgcggaa ccttggagtt gtttaggtat ccggatgaac aaccagaccg cagccgtggc
     5041 gttgcatcgg atgagatggc gtataagttc taccttttgg taagacatgt caaccagaat
     5101 tctgaaattg gttaaccgca tcgcaagaat ttcactctca tggttgttgc tgcaaattgc
     5161 agcaaagggt aatcgatggt tattgatcag aggatgtgac attcatcgca acaatggttg
     5221 accaatttac ataacatgtc gcccaaaata acaacggttc aaccaagtaa ggtaaaacgt
     5281 tatgcatgtg cttaacattc tctgggtggt attcggcatt ggcctgatgc tgatactgaa
     5341 tttgaagttc aaaatcaatt caatggtggc tttgttggtg gcggcgctgt ccgtcgggat
     5401 gctggcaggc atggatttga tgtcgctgct gcacaccatg aaagcgggct tcggcaacac
     5461 gctgggagaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga
     5521 ctccggcgcg gctcaccaga tagcacatac gctgctggcg cgtctcggtc tgcgctatgt
     5581 acagctgtcg gtgattatca tcggccttat tttcggtctg gcgatgttct atgaagtggc
     5641 ctttatcatg ttagcgccgc tggttattgt tattgccgcc gaagctaaaa ttccgttcct
     5701 gaaactggcg atcccggcag tagcagctgc cactaccgca cattcactgt tcccaccgca
     5761 gccgggtccg gtggcgctgg tgaatgctta tggcgcggat atggggatgg tttatatcta
     5821 tggcgtactg gtgacgatcc caagtgtaat ctgcgcaggt ctgatcctgc cgaagttcct
     5881 cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg tagatatgaa
     5941 taatctgccc tctttcggcg tttcgattct ggtgccgctg atcccggcga tcattatgat
     6001 ctctaccacc atcgccaata tctggctggt aaaagatacc cctgcctggg aagtggttaa
     6061 ctttattggt tcctcgccga ttgcgatgtt tattgcgatg gtggttgcat tcgtactctt
     6121 tggcaccgcg cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa gcgcggtgaa
     6181 gagtattgca atggtgattc tgatcatcgg tgcgggtggc gtgctgaagc agaccatcat
     6241 cgacaccggc attggcgaca ccatcggcat gttgatgtcc cacggcaata tctcgcccta
     6301 cattatggca tggctgatca ctgtgctaat tcgtctggcg acgggtcagg gtgtcgtttc
     6361 ggcgatgacc gccgccggga ttatcagtgc tgcaatcctt gatccagcaa ccggtcagct
     6421 ggttggcgtg aatccggcgc tgctggtact ggcgacggct gcgggttcca acaccctcac
     6481 ccacattaat gatgcatctt tctggttgtt caaaggttac tttgacctgt cagtaaaaga
     6541 cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc tgattattgt
     6601 gctgattatt agcatggtag cgtaaaagaa caccagagcc tgccaatggc aggctcagac
     6661 tgatgactaa agtctaaaaa tgtgcccgga cagtcccctc gcccctccgg ggagagggtt
     6721 agggtgaggg gaacagacca gtaaagtgcg ctttgtttat gccagatgcg acgctgacgc
     6781 gtcttatctg gcctacaaag ggctaacgtg caggttttgt aggtctgata gcgtagcgcc
     6841 tcaggtacca gcattagcaa tgcgctgtaa tttttttatt gataaacaaa agtcacgcca
     6901 ataatcgatt gcacattccc tgcagtcacc tgccctccgg tacgtgcgta atttgccgtt
     6961 aatcccagac ttaccgccga agttcctact gctcctaacg ataccgtgtt attcgctgga
     7021 ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gcgctggtga aaacgacgcg
     7081 gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta ataccccagg
     7141 ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg gtagtccggc
     7201 agagtaacgg tgacatcacg agcagaaaca tcacaaccgc cagtgggcac caccacatca
     7261 ttattggcgt aaatattcca cacaaactga aaatcatcgc tgttatagtt gttggtctgt
     7321 cgcaaaataa gcacggcaat taatgaacca gctttaatcg ccaccccgcc cgcactgctc
     7381 acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt ataaacaacc
     7441 cgcggcgttt cgctggtggt cggaaatgga tagctactgc cactatattt tacggtcccg
     7501 gaaaaattag ataacacgcc gccgtaagcc gagcctcgtt gcagtgtgac atagtctgta
     7561 atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatctac gaccaggttt
     7621 tgccccacat tcacgacagg cgcaaggttt acataaacat tagcgctgcc accgccaata
     7681 gggatagcgg taccattggc ggttttacag gcgaatgacc aggcatttac cgaccagccc
     7741 atcagcagta cagcaaacag ggtaatagct cgtttcatta caattatctc ttcgggttca
     7801 gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg ctccgccatt
     7861 taccgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat catccacctg
     7921 aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta gctctaactg
     7981 gatgttttgc gcggtcccct ggtttttata atatccggtg ctgtcggctg ccccgctgaa
     8041 gctggcagtg accctcgacg ttcccaccgg acaattagtc aactcaagcg caacatcatg
     8101 ccaggccgat gccgccccgg cagacataag actgaaagaa taaagatcgc cgagatctac
     8161 cgtggcattg gtggtggaaa ccgtacacgg tttggcgacg accttaccgt tcaccgtgat
     8221 ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aacattgccg ccaatacata
     8281 cccacgtttg caccatttca tgagcatctc cagttactga tattcaagag tgaaggtagc
     8341 cgtagcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa
     8401 attcagcgta tttggtttac ccggcgtcag ggtcgtccac gaaatcgcag acgatggagc
     8461 attaaggggt atttgatttt gctgctcatt cagaagctgt attcccagtc ccgcagccgc
     8521 tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa cgccggtaaa
     8581 cccaactttt acggcagaaa cggcattacc acagggtgac agcaaaatac gaaatggaac
     8641 gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac
     8701 agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat agccgcggat
     8761 agtaatcgtg ctatccgcag ccaaaacgcg acttaccgcc agccacaaaa aagcgcacag
     8821 aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg atagctgggt
     8881 taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt gagcattttc
     8941 ctcttctccc catttcacct gaacttttcc cgctaaaggc attccgctga ggtaaacctg
     9001 accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca tcgccccaaa
     9061 cggcagcggc ttattattgt gagttagcgt catgagcagt tttatcccaa cgcgcgcttt
     9121 aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg cgttatctaa
     9181 atcgacgtta tcagccaagg tattggtatc cagcgccact ctattttccc gatattcagt
     9241 ggcataaggc agcacggcat aaccacgcca gtcggtacgc acccccgtct ggttttcgac
     9301 ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta acggctgccc
     9361 cagcgttacg ccattggcat gagccaatac cccaccgctg actccgtaat agagctgctt
     9421 aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc gataattcag
     9481 cgtggcgtag cctgtacttc cgctattacc atcgcctccc ccggcatagc cggtttgcac
     9541 gctataactg aggttgttgt cttccagcaa cgtaccgtat acaccagcca gattggtcat
     9601 ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc actgagattt
     9661 actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttaagcgcta acatctgatc
     9721 ccgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt tgatatcttc
     9781 gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc cccaataagt
     9841 ttgatggcta ccactcaaat acagtgttga tgtgcgcccg agttgctgag taacggtgag
     9901 ctgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt tcggcttaac
     9961 ctgaataact ccatcctgtg tttcgatgtt gtagccattc attcgactgt atgttgtatc
    10021 agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa tattcgtgcc
    10081 tgattcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat gctgactgtc
    10141 atcgggaagt gtggaattag cctgcgtcat atccacagac agagcgccca gtgcctccat
    10201 gtttttcccg ataccgaaat taaaagcacg ataacgatcc gccagttgcg ttccaccata
    10261 tattgtccag ccagccggaa ggccgtggag taatgtactc tggaaaaagc ggggtttttc
    10321 ctgctgcgca tttccactac ggtattctcc tgccgtaatg gaataacgag tatgcccttc
    10381 acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc tgccgtcagc
    10441 ctctttgatc gttacctgca agtcaccact attacctgcg gcatagatat cgttgatggt
    10501 aaaaggcccc ggtggcaccg tactattata aatgccatat ccattttgtt taatagtgac
    10561 ctgtgcagta ccacgagcaa taccgtagat caccggggca aatcctcttt ggctatcggg
    10621 taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat cgaaaatatc
    10681 gccctgggta taaccatcac ccaacgtcag ccgggaacgt aaaggaatta tgtctcgctc
    10741 aatccaggta atgatatgct gccatttatt tttgctacct gatgaactgt cgctactgtt
    10801 ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc cactctgtag
    10861 gtttaaatat gcataatggc tgttaccccc aatccgattc tgtacactat ttccgctgaa
    10921 attataattg agcaatccgg cattaatacc gggatcccat aactcaggag gaatataacc
    10981 acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttcagtcgct gctgaccaac
    11041 atccagatgc gcagtagcgt cttggaccat tgtggttaat ggcacacagg catcatccgc
    11101 cagcagattc ataccggcga cagaagccgt attcagcccc atactggcga gttgcgcgcg
    11161 tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga catcacgcgt
    11221 tgccatataa ccattattca aatagatatc gacgcgatac gtccctggcg gtaattcttg
    11281 cccattttca aaacgcgata aatcggccac agcctgggga tcatccgcta aaaagcgcag
    11341 attaaaatag aggtcggcag atgacaaagg tgcctgtgcg gcaaaagcac aggcgacaac
    11401 gagtcggaca aaaaaaccag ccaaacgatg cttacgaata tgcaagcatt gtgtgtttcg
    11461 ctggtaaagt cttaaattca gatatgacat tattcctccg gtttaaaatc acccggcagt
    11521 caattctttt tattcaggcg aaaaattccc cctgcgttat tccattacgc ccgtcatttt
    11581 gggggtaagt gcgccataat catttattgt tcggtaagta atattgcttc ctgcatcaga
    11641 aggcaattta accgtgcttt cgcccattgg aggcaccaat gcattttcaa gaacccgggt
    11701 tccggcattc aactctgtta ccgtcaggta atagggtgtc gggttaatca gcgtcagaga
    11761 attcgcgcta cgacgaaatc ttaatttttc tgcggcctga tcgggtggca acgctaattt
    11821 agccgggcga tagtacagtt taatgcggct gataattgcg agctgtagca tattctcagt
    11881 caattttgat ttatccattg acggaatcgc tttaacgttc atccagaata aactttcccg
    11941 gtcctgtggc aattggttat ttgttgcatc aagaatacgt aaggtattct ctttttttcc
    12001 cttcatcgca aacagaggag gcgtcacgat aaaacgacca tcctttacac catcggcatt
    12061 ttccacccat gattgaatta aataggtact attttcatca ttatttgtca cggcaagttg
    12121 cacttgtttt taccctgccg gataaattac gcgagtcgca cctaaggcca ctcctgcttc
    12181 agcgcgtccg gcaaccatca ttgccatgaa catcaggata cctgccagca agcagaatgt
    12241 tatttcctgc gatttcctta cattgacgtt tttattactc actgtcctgt tcctgttatc
    12301 acattatcta ctgaacaatt actgataggt taaagagaac caggcctggg cattggcgat
    12361 gccgccagta acccgacgcc cggtagcacg atatttggcg atgaaatgta gcgaagtaga
    12421 gcctgagtaa agccgtttcc agtttgctgg aggacgatta atcggtacga ggtttccttc
    12481 atcatcaaac aacgctacac caatattgga ggctatccct ggcccctctc ccacggagag
    12541 cacatccgga tttttaccat ccgcgacacc gtgaaacgcc acacccacac gttcactcac
    12601 caccgtgcta cattcccgta aatgaataac aaaaggcacc ggtgcgctat cttcccctgc
    12661 cgcatgaaac cggttactgc tgatttgccc catattgacc gtcatttgtt tatcaccggc
    12721 ttcaatccgg caagtttccg caataatgac gccctgaaat tgcatatttc cgccgggcag
    12781 cgtggtattc catttatttc cggccagagc aaacattggc agcaacgacg ctaacagaaa
    12841 taatctcttc cttttcatcg ttatttttat cgcacaaggg tgggcatccc tgcccgtaat
    12901 gacgtccttg aacctgggta ggttattgat actgaacctt gaaggttgca tccgcattag
    12961 ctgcacccgg ggttgcctcg ccgattgcat aataacgcgc ctggaacgga atggtgttgg
    13021 taccgttatt cagggttgtt tgtgcactaa atgtcgcacc gtccagcgtc agagcattgc
    13081 ctgttctgtc caggatctgc acgccaacgt ttgttgcact acctgcagca gaactctgca
    13141 gagccagtac atcagtacgc gtagcatcaa ttgccgtacc taagaaggca acagcggctt
    13201 ttgtggcaac agtggtatcg caatcattca gctgaatgtt aaaaccaacg gcagagctgg
    13261 ttgctccagc ctgcttcagg ctagcggtac gaacctgtcc caactgaacg gtttgatcaa
    13321 cagagcctgc atcaactgcg caagcggcgt taacaacttc ccctttaaag tgaatggtcc
    13381 caccatttac cgtcgtagta tcggccagag ccgctgcgga actgagggac agagccgaca
    13441 gaacaacgat tgccagagtt ttaattttca tgctgctttc ctttaaaaaa actatttcta
    13501 aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca acgagtttga ctgccaacac
    13561 tgcacagttt tcccccaaaa gatgaaacat ttggggccat tttgactcat agaggaaagc
    13621 atcgcgcaca aactttttca gtttatttgt tggcttaata ttctattatt atctttattt
    13681 atagattttt atattgcatg aggtagtttt tggagagaag aatgaggaag atgcgtcgag
    13741 ccacagaaac gttagcttta catatagcgg aggtgatgtg aaattaattt acaagagaaa
    13801 taatttacat atcaaatagt tagatgcttt tgtcgttttt taatattttt atgcttgaga
    13861 aaaaatacgt aacttattta tgatatggac agtttggccc caattgtctt gtgtttattt
    13921 gttttttaaa tttattatca ataagttaaa tcaaacctct tctcttttta atttttcgtt
    13981 tatgagatta tttctttccc ataatccggc aaaacgagca gcattactgg cggtataacg
    14041 cacagtatgg cgaatatttc gattcccgag ataatcctga attaaacggg tatctgcacc
    14101 acgctccgcc agttcataac cgcaagcatg ccttaacata tgaggatgag tctgcgttac
    14161 ggttccagct tcaataccgg catcgcgaat aatgcgatag gcctgctggc gagaaagccg
    14221 actcccgcgg cgagaaataa atatagcgtc ggtccggtca gcgcctttcc agttagcacg
    14281 ttcctgggtc cagcgttcca cggcttcacg ctcatcaaaa cgtaacgggt gaacggtaga
    14341 aaatccgttc ttcagtcggc gaatatttat tcgaccttca ttaaggtcaa ggtcctgata
    14401 atgcagatca agcagttcac taatacgcat cccatgccga tatgccaaca gaataagaca
    14461 ataatctctg gctcccgttg ccccgtaaca aaccgcctgc atcatggcct gaacttcttt
    14521 accggtaaga taacgacgtt tactcacaat agtagtactc ctaactgaga tatatttgaa
    14581 tgaataccta taggaaacct caatcggtca aaattagccc aatagataaa aagataacca
    14641 cacacgaagt tatgtggcta tcagtcaatt acattaaaca tcatacaaaa ataaaatatc
    14701 actgatgtga caacatctat tttcattgat ttgattcaat ataacaatag attacagagg
    14761 attaacttcc agttacttaa aaaataaaga aaaaagtgtg gaaacaggag aatttaagtg
    14821 atgtgtaaac ttatgaatga ttttgtttat ttattttgaa gaacataaac cattagattt
    14881 tgtatgggta tggttaactt aatgtttatt atgataatta agaatcacac ttacaataac
    14941 atcaattaat aaatggttaa tcatttttga tatatcgtaa gaataatgta gtttttaaca
    15001 ccatccctgg tatctcaact atctctataa aacagcatga cgctgtcgtc ctctggctct
    15061 atcccagatg ccgtaaaaac gccctgcatt gctggcggta taccagacag tatgacgaat
    15121 attgcgatgc ccaagataat cctagataag tcgcgtatct attcccatat tcgccaaagc
    15181 aaaaccacac gaatagcgta acatgtgcgg atgaatctcc agtgacaacc cggcattacc
    15241 accagaagtc gagataatat ggtaaaactg ttgccgagaa agcggattcc ccttacgtga
    15301 taaaaatacc cactcgctct cagcatgcgg ataagaagta cggatactca accagttttt
    15361 taaagcctga acttctttgt tcaatagcgg gtgcgttgtt gaaaagcctt tttttaatcg
    15421 atggatatat atacactttg ctttaagatc aatatccgaa atcctcaatc gacaaatttc
    15481 actcgcccgg aaaccatgaa taaaacaaag caaaatcaga caataattac gtgttgcatg
    15541 aggcccggta tttgctgctt taaggagtga ctcgatttca ctatgggtaa ggaagttcct
    15601 ttttttgtta tcagccttat tcctcatcgt tttcccttat aattacagac gcgcactagc
    15661 tgtgctgggt tagcaataat ccaacatttt tatcctgatt atgtttataa aagcgaacgt
    15721 ttgcttaata acttacgtaa gtggaccagt tcttctgagc gaacttaaat ggagtagcga
    15781 tagttactta taataacttt gccatacaaa cgccatgctc ttgcatgcta tgtaccttta
    15841 tatatttatc aatctgatac gaatctaata ataaccctca tcattaatgc aactaatctt
    15901 atctatatat catgtgatat gttctgtaac aagtaaaatc aacataaaaa attcagcaaa
    15961 atttaatatt tgttaaacta gccgtctcaa atatgacatt attagttaac tgcatttgcc
    16021 acaaaatggc tatttcatgg aacgcagtaa tttcttagtg gctattatca tgctaattct
    16081 tagatcccta gcgattattc ctgcctggtt cgtcagctta ggccatgaga ttgttacatt
    16141 cctttttatc ctggagatat gggggaacgc aggcgacgct ttttcatcta catccgttgg
    16201 taaatatagc tttggtcact acgacttaag ccttcctcaa tcacagctat tcgccgggcg
    16261 atacggcaaa caaactaagt gaaacatcat cctgaaaaca ccaacatcaa caagcctctc
    16321 cagatcgaca tcaggagtga ccagttacaa gccacaaacc aagaaacata tttcatgatg
    16381 agaattatgc tcagcctgat ggcgagtaaa cccttatttt caccactctc actgagttag
    16441 cttgcaaagc tttctttgtt tagattctaa ataaattatt tataaaacat atagttaaaa
    16501 gaaaatcttt agtttttgaa gaccaccgca agtgttcgtc tggcttcaca tggcatcttc
    16561 ttcttagaaa aagatcgaca tattttgtga cgcgaattgc aaatctggtt ttgttgtatg
    16621 gattgcgtga tttttgatct ggtataacag gtataaagat gcaccacgat agtcaatgag
    16681 actgggcatc tcgtaatcta tggcaaacat cacttcagtt ctttcacatc gggtgatgaa
    16741 aatgcacttc agtctgaaag gaatatgaaa atgagatcaa cagacactct attttatgac
    16801 tctgggtaaa atgggttgag taagtgatat aacttacgaa tattcaaatc aattaaaaat
    16861 cagaagagat tttatactca ggtatttaat ctggatctct gtttatttaa ataatgttaa
    16921 aagagaattt tcacaggaga ccttatacaa aaaatataaa atacagttac ccgttgccaa
    16981 agacactata agcctggcaa aaaaatatta cacaacataa atgctaattg tttatgcggg
    17041 ctttgtattg ctttctgtat cctacaaatg agtgaaattt atgaaaaagg ctaaaatact
    17101 ttctggcgta ttattactgt gcttttcgtc cccattaatt tctcaggctg cgacactgga
    17161 cgtacgtggt ggatatcgta gtggaagcca cgcctatgag actcgactca aagtcagtga
    17221 gggatggcaa aatggatggt gggcaagcat ggaaagtaat acctggaata ccattcatga
    17281 taataaaaag gaaaatgccg cactcaatga tgttcaggtt gaagttaatt acgcaattaa
    17341 acttgatgat caatggacgg tgcgcccggg aatgttaacg cattttagca gcaacggcac
    17401 acgctacgga ccctacgtaa aactgtcctg ggacgcgaca aaagatctta attttggcat
    17461 tcgctatcgt tacgactgga aagcttaccg acaacaagac ttatccggtg atatgtctcg
    17521 tgataacgtt catcgttggg atggatatgt cacttaccat attaatagtg atttcacctt
    17581 cgcatggcaa acgacgctat acagcaaaca gaatgattat cgctatgcaa accacaagaa
    17641 atgggcgacg gaaaatgcct ttgttctaca ataccatatg acgcccgata ttacgccata
    17701 catagaatat gactaccttg accgccaggg tgtttacaac ggcagagata atttatcgga
    17761 aaacagttat cgcattggtg tgtcatttaa actgtagtag acaggagaca gtcacaatga
    17821 ataaaactat aatggcgctt gctatcatga tggcttcatt tgccgcaaac gcgtctgtat
    17881 taccggaaac tcctgtgcca tttaaaagtg gtaccggagc aattgataac gacactgtct
    17941 acattggttt aggtagcgca ggtacagcat ggtacaagct agatacacag gccaaagata
    18001 aaaaatggac tgcgttagct gcattccctg gtggaccgag agagcaagca acctctgcat
    18061 ttattgatgg caatctgtat gtgtttggcg gcattggcaa aaacagcgag ggattgactc
    18121 aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa tagctgggtt aaattgatgt
    18181 cgcacgcgcc gatgggcatg gcgggtcatg tgacttttgt acacaacggc aaggcttatg
    18241 ttactggcgg tgttaaccag aatatcttca atggctattt tgaagatctc aacgaggctg
    18301 gaaaagattc aaccgctata gataaaatca acgcccacta ttttgacaaa aaagcagaag
    18361 attatttctt caataagttt ctgttgtctt ttgatccctc aacacagcaa tggagttacg
    18421 ctggcgaatc gccctggtac ggaacggctg gtgcggcggt tgtgaataaa ggtgataaaa
    18481 cctggcttat taatggcgaa gccaaaccag gattgcgaac ggatgccgta tttgaacttg
    18541 atttcaccgg taataattta aaatggaata agcttgatcc cgtctcatca ccagatggcg
    18601 tcgctggcgg ttttgcgggg ataagcaatg attctcttat atttgccgga ggggccggat
    18661 tcaaaggttc acgagaaaat taccagaacg gtaagaacta tgcgcatgaa ggcctgaaaa
    18721 aatcatatag cactgatatt catctttggc ataacgggaa atgggataaa tcgggtgaat
    18781 tatcgcaagg tcgggcctac ggagtatcat tgccctggaa taatagttta ttaattattg
    18841 gcggtgaaac tgcaggcggc aaagcggtga cggattcagt tttaatttct gtgaaggata
    18901 ataaagtcac agtacaaaac taacgcttca gggccccggt aaaggggccc tgctatcaat
    18961 ttgtcatttg tattacgcga aaattatatg aacgcaataa tatcacccga ttattactat
    19021 gttcttaccg ttgctggtca gtctaatgcc atggcgtatg gcgaaggact gccattaccg
    19081 gacagggaag atgcgcctca ttccagaatt aaacaattag cgagatttgc gcatacgcat
    19141 cccggaggcc cgacatgtca ctttaacgac attattccac tgactcactg cccacacgat
    19201 gttcaggata tgcagggtta tcaccatcct ctggcaacga atcatcaaac acagtacggc
    19261 accgttggcc aggcactgca tattgcacgg aaattactgc ccttcattcc tgataatgca
    19321 gggattctta tcgttccgtg ttgccgtggc ggatcggctt ttaccgcggg cagcgaaggg
    19381 acatattcag aacggcacgg agccagccat gatgcttgtc gttggggaac ggatactccg
    19441 ctgtaccagg atttagtcag cagaacgcga gccgcactgg caaaaaatcc gcagaacaaa
    19501 ttcctcggcg tatgctggat gcaa
//
LOCUS       DAJTUN010000080        19278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTUN010000080 DAJTUN010000000
VERSION     DAJTUN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.080"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(23..99)
                     /locus_tag="OO470_002579"
     tRNA            complement(23..99)
                     /locus_tag="OO470_002579"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Met,seq:cat)
     gene            308..1405
                     /gene="mltA"
                     /locus_tag="OO470_002580"
     CDS             308..1405
                     /gene="mltA"
                     /locus_tag="OO470_002580"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6251790.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1644..2450
                     /gene="tcdA"
                     /locus_tag="OO470_002581"
     CDS             1644..2450
                     /gene="tcdA"
                     /locus_tag="OO470_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6251791.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2501..2944)
                     /gene="csdE"
                     /locus_tag="OO470_002582"
     CDS             complement(2501..2944)
                     /gene="csdE"
                     /locus_tag="OO470_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6251792.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2944..4149)
                     /gene="csdA"
                     /locus_tag="OO470_002583"
     CDS             complement(2944..4149)
                     /gene="csdA"
                     /locus_tag="OO470_002583"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6251793.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4341..4568
                     /locus_tag="OO470_002584"
     CDS             4341..4568
                     /locus_tag="OO470_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6251794.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4919..5836
                     /gene="gcvA"
                     /locus_tag="OO470_002585"
     CDS             4919..5836
                     /gene="gcvA"
                     /locus_tag="OO470_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6251795.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5855..6250
                     /locus_tag="OO470_002586"
     CDS             5855..6250
                     /locus_tag="OO470_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6251796.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6243..7343
                     /gene="rlmM"
                     /locus_tag="OO470_002587"
     CDS             6243..7343
                     /gene="rlmM"
                     /locus_tag="OO470_002587"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6251797.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7387..8118)
                     /gene="fucR"
                     /locus_tag="OO470_002588"
     CDS             complement(7387..8118)
                     /gene="fucR"
                     /locus_tag="OO470_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6251798.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8176..8598)
                     /gene="fucU"
                     /locus_tag="OO470_002589"
     CDS             complement(8176..8598)
                     /gene="fucU"
                     /locus_tag="OO470_002589"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6251799.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8600..10019)
                     /gene="fucK"
                     /locus_tag="OO470_002590"
                     /pseudo
     CDS             complement(8600..10019)
                     /gene="fucK"
                     /locus_tag="OO470_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10128..11903)
                     /gene="fucI"
                     /locus_tag="OO470_002591"
     CDS             complement(10128..11903)
                     /gene="fucI"
                     /locus_tag="OO470_002591"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6251800.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11936..13252)
                     /gene="fucP"
                     /locus_tag="OO470_002592"
     CDS             complement(11936..13252)
                     /gene="fucP"
                     /locus_tag="OO470_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6251801.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13799..14446
                     /gene="fucA"
                     /locus_tag="OO470_002593"
     CDS             13799..14446
                     /gene="fucA"
                     /locus_tag="OO470_002593"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6251802.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14474..15622
                     /gene="fucO"
                     /locus_tag="OO470_002594"
     CDS             14474..15622
                     /gene="fucO"
                     /locus_tag="OO470_002594"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6251803.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15675..16430)
                     /gene="xni"
                     /locus_tag="OO470_002595"
     CDS             complement(15675..16430)
                     /gene="xni"
                     /locus_tag="OO470_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6251804.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16541..17908)
                     /gene="sdaB"
                     /locus_tag="OO470_002596"
     CDS             complement(16541..17908)
                     /gene="sdaB"
                     /locus_tag="OO470_002596"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6251805.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17966..19255)
                     /gene="sdaC"
                     /locus_tag="OO470_002597"
     CDS             complement(17966..19255)
                     /gene="sdaC"
                     /locus_tag="OO470_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6251806.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4701 a         4763 c         5119 g         4695 t
ORIGIN      
        1 cttttacttc atcaaatttg attggttgcg ggggccggat ttgaaccgac gaccttcggg
       61 ttatgagccc gacgagctac caggctgctc caccccgcgt ccgtggatgc gcactatact
      121 ccgctcgcgt tttcatgcaa cctttttttt accccgatca cggatttagc atgattttga
      181 acaaaaacaa accactaaag cctatttttt gtgcaaaatt tgcgtcaggg agaggctgct
      241 catttcatta aacgcggcgg tttgttatct tcgttgcgcc ttatttttta acctgaagaa
      301 gagaacaatg aaaggacgtt gggtaaagta ccttcttatg ggcacggttg tggcaatgct
      361 tgccgcctgc tcttccaaac caaccgatcg cggacagcaa tataaagacg ggaaatttac
      421 ccagcctttc tctctggtga accagccaga tgccgttggc gcgccgatta acgccggtga
      481 ttttgccgag caaattaacc atatccgtaa ttcgtcaccg cgtctgtatg gcaaccagag
      541 taatgtttat aacgcggtgc aagagtggct gcgcgcaggc ggtgataccc gcaatatgcg
      601 ccagttcggc attgatgcct ggcagatgga aggtgccgac aactatggta acgtgcagtt
      661 tacaggctat tacacgccgg taattcaggc gcgccatacc cgccagggcg agttccagta
      721 tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg tctcgtgcgg agatctacgc
      781 cggggcattg ggcgataaat acatcctcgc ttacagcaac tctctgatgg ataacttcat
      841 tatggatgtg cagggtagtg ggtatatcga ctttggtgat ggcagtccgc ttaacttttt
      901 cagctatgca gggaaaaacg gtcatgccta tcgcagcatt ggtaaggtgc tgatcgaccg
      961 tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt cgtcattggg gcgaaacaca
     1021 cagtgaagcc gaggttcgcg agctgctgga acagaacccg tctttcgtct tctttaaacc
     1081 gcaatctttt gcaccggtga aaggggcaag tgcggtgccg ctggttggcc gtgcttctgt
     1141 tgcctccgat cgttcaatta ttccgccagg tactaccttg ctggcagaag tgccgctgct
     1201 ggataataac ggcaaattta acggtcagta cgaattgcgt ctgatggtgg cgttggatgt
     1261 cgggggcgca atcaaaggcc aacacttcga tatctatcaa gggatcgggc cggaagccgg
     1321 acaccgcgca ggttggtaca accactatgg acgggtttgg gtgctgaaaa cggctccggg
     1381 cgcaggtaac gtctttagcg gctgatgtga tattctgtat gccgttttta cggatatgac
     1441 agggtgaggg gtaacctcac atcctgatta ttgactaagt gcgcgttgtt cataccggat
     1501 gcggcgtgaa cgccttatcc ggcctacaaa tcatgcaaat ccaaaatatt gcaggagtta
     1561 tgtaggcctg ataagcgtag cgcatcaggc atttatgcgt ttagggtgag ggataacctc
     1621 gcccttttta attctgaggt gctatgtctg tggtaattag tgatgcatgg cgtcagcgtt
     1681 ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca actgtttgct gacgcgcata
     1741 tttgtgtggt tggtatcggt ggtgtcggtt cctgggcggc ggaagcgctg gcgcgcacgg
     1801 ggattggcgc aatcacgctt atcgatatgg atgatgtgtg cgtcaccaat accaatcggc
     1861 aaattcacgc tctgcgcgat aacgtcgggc tggcaaaagc ggaagtcatg gcggagcgca
     1921 ttcgccagat taacccggag tgccgtgtaa cggtggtgga tgatttcgtg acgccggata
     1981 acgtagcgca atatatgagt gccggttatt cgtacgtgat tgatgccatt gatagtgtac
     2041 ggcccaaagc ggcgctgatt gcttattgtc ggcgcaataa aatcccgctg gtcacgaccg
     2101 gtggcgcggg tgggcagatt gacccgacgc agattcaggt taccgatctg gcgaaaacga
     2161 ttcaggatcc gctggcggcg aagttgcgcg agcgcctgaa aagcgatttt ggcgtagtga
     2221 aaaacagtaa aggtaagctc ggcgtggatt gcgtgttttc tactgaagcg ctggtgtacc
     2281 cgcagtcaga cggtacggtg tgtgcgatga aagccacggc agaagggccg aagcggatgg
     2341 attgtgcatc tggatttggc gcggcaacga tggtgaccgc cacctttggg tttgttgcgg
     2401 tttctcatgc gctgaagaag atgatggcga aagcggcgcg tcagggttaa acaatcaggt
     2461 cggatgagac gcgaacgtct tatccgaccc ggttctgtgc ttaaacctgc ttcgtcgcag
     2521 cgataatcgc ctcgcttaac gcatttaacc cctggctgcg tgaggcgcta agctgcgcac
     2581 gtaatcccag ctcatcaaac aatgccagcg gcgactgtgc ctgtaattcg gcggcggttt
     2641 tcccctcaac ggcagtcaac aacaccgcca acaggccgcg cacaatgcgc ccttcgctgt
     2701 tgccaaagaa gtgcattttg ccgttttcag ccacggtata tcccagccag acgcggtttt
     2761 cgcatccggc aatctcttta gcctgcgctt ttaactcgtc tggcaaagcc ggaagctgtt
     2821 tccccagcat gatcaactgg cgatatttat cttcccattg cgacaacggt gcgaaggtat
     2881 tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg tccggcgaat tgcgggtttg
     2941 tcattaatcc accaataatt ccagcgcgcg gtcaacggca ttcaccagcg catccacatc
     3001 actctttgta ttatatggcg caaatgaggc gcgcagtgtg ccggttacgc ctaattctgc
     3061 cagtagcggc tgagcgcaat gttgcccggc ccgcaaggca ataccgtact ccgccagtag
     3121 cgtcaccata tcgctgtgat gaacgccagc aaaatcaaag gccagcaggc tggaatcctg
     3181 gcagcggaat gaacgaaagc ctggacgttt cgccagcgct tcttctgcaa gcgttgctaa
     3241 gctacggctc cagctttcgg cctggttgat atcgtaatct gccagccatt ccagcgccgc
     3301 gcttaatcct atgacgccag cgacatttgg cgttcctgct tccagtttcc acggcgcaga
     3361 ttgagtggtg aaaccgtcaa aactcacttc gtgaatcatt ttgccgccac ccagccaggg
     3421 cgacatcgct tccagcagtt ctgatttgcc atacagcacg ccgatacccg tcgggccata
     3481 cagtttgtga ccagagaaag catagaaatc gatatccagt tgctgaacat ccgcggggaa
     3541 atgcaccgcc ccctgagcac catcaaccat caccaccatc ccggctgaat gagcaaaggt
     3601 aatcgctcgc gccagatccg ggcaaccgcc agtcacgttc gacatctgac ccaacgccag
     3661 aatccgacta cggggagtaa tcagctctgg caacaaatcg acatccggca gtcgctgcgc
     3721 attaagcggc aatttcacca ctttggctct agtttgttgg gcgaccatca gccaggggac
     3781 gaggttagcg tggtgttctg ccacgctgac aataatctca tcgcccggtt gcagacgcgg
     3841 acgcgcatag cattgtgcca ccatgttgat ggattcagtg gtgccgcgcg tccagacgat
     3901 agttttatca tccggtgcat tcagtaattg cgccactttc tctcgtgcag cttcataacg
     3961 cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg acgtttccgg cgctcaggct
     4021 gtaaaactgt cgagtggctt caaccacggc ttcaggttta agcgcggtcg cggcgctgtc
     4081 gagatagacg cccgcatcct gtagtgcggg aaactgggcg cgaaactgcg cgggattaaa
     4141 aacgttcatg gtactcctcg gcttgatgca ccgatcgtga cgcaaaattc actttgatac
     4201 aaggagagtg ctaaccatca gaattatctg gatatcctgg cgaagccgcg taagtaacat
     4261 atgctgaata gtgttaggcg ttaattttag cctgttaatc aatactatta agatttatta
     4321 atcgttttaa ggagaagatg atgaaaaaga ctgccgcaat tatttctgcc tgtatgctga
     4381 cttttgccct gagcgcctgt tccggttcga actatgtgat gcacaccaat gacggacgta
     4441 ccatcgtctc tgacggcaaa ccacagactg ataacgatat cggtatgatt tcgtataaag
     4501 acgctaatgg caacaaacag cagatcaacc gtactgacgt gaaagagatg gtcgaactgg
     4561 aacagtaatt cgcgatcgca aggtaaaaaa aagcaccgca attaggcggt gctacattaa
     4621 tcactatgga cagacagggt aaatgtacag gaagtgaaaa aaggtagctt tgctaccatg
     4681 gtctgaatcg cagaccaatt gcaaacacaa caacacaaca tcacaaccgt aagccaaaag
     4741 ttcaccagaa tacgcattcc gataaaactt ttcgttccgg ctcaggaagt gccgccacta
     4801 taggtatttg ctggtagaag ctcaacggac aatttataat ggctcagatt aaaaaaacta
     4861 ataggttaca cagtgtgatc taattgttaa attcatttaa catcaaagtt taaaagccat
     4921 gtctaaacga ttaccaccgc taaatgcctt acgagttttt gatgccgcag cacgccattt
     4981 aagttttact cgcgcagcag aagagctttt tgtgacccag gccgcagtaa gtcatcaaat
     5041 caagtctctt gaggattttt tggggctaaa actgttccgc cgccgtaatc gttcactcct
     5101 gctgaccgag gaagggcaaa gctatttcct cgatatcaaa gagatatttt cgcaattaac
     5161 cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg gcgttgacgg tcagtttact
     5221 ccccagtttc gccattcatt ggttggttcc gcgactttcc agctttaatt cagcttatcc
     5281 gggaattgac gttcgaatcc aggcggttga tcgtcaggaa gataagctgg cggatgatgt
     5341 tgatgtggcg atattttatg gtcggggcaa ctggccgggg ctacgggtgg aaaaactgta
     5401 cgccgaatat ttattgccgg tgtgttcgcc gctactgctg acaggcgaaa aacccttgaa
     5461 gaccccggaa gatctggcta aacatacgtt attacatgat gcgtcacgcc gtgactggca
     5521 gacatatacc cgacagttgg ggtttaatca tatcaacgtt cagcaagggc caatttttag
     5581 tcatagcgcc atggtgctgc aagcggctat tcacgggcag ggagtggcgc tggcaaataa
     5641 cgtgatggcg caatctgaaa tcgaggccgg acgtcttgtt tgcccgttta atgatgttct
     5701 ggtcagtaaa aatgcttttt atctggtttg tcatgacagc caggcagaac tgggtaaaat
     5761 agccgccttt cgccaatgga tcctggcgaa agccgctgct gaacaagaaa aattccgctt
     5821 tcgttatgaa caataattta cgtagggtac gaccatgacc agccgtttta tgctgatttt
     5881 cgccgctatt agcggcttca tttttgtggc actgggcgct tttggtgcgc atgtgttaag
     5941 taaaaccatg ggggccgttg agatgggctg gatccaaacc ggtctcgaat accaggcgtt
     6001 tcatacgctg gcgatcttag gtctggcggt ggcaatgcag cgtcgcatca gtatctggtt
     6061 ttactggagt agcgttttcc tcgcgttagg cacggtgctg ttcagcggca gcctttattg
     6121 cctggcgctg tcccatctgc gtttgtgggc gtttgtcaca ccggttggcg gcgtgagctt
     6181 ccttgcgggc tgggcgttaa tgttagttgg tgctatccgt ttaaagcgca agggcgtaag
     6241 tcatgaataa ggttgtattg ctgtgccgtc cgggctttga aaaagagtgc gccgcagaaa
     6301 ttaccgataa agccggccag cgggaaattt tcggttttgc ccgcgtgaaa gagaatgcgg
     6361 gttatgtcat ttatgaatgt tatcaacctg atgatggcga taagttaatc cgtgagctgc
     6421 cgttcagttc attaattttt gcccgccagt ggtttgtggt gggggaactc ctgcagcatt
     6481 tgccgccaga agatcgtatt acccccattg tcggcatgtt acagggtgta gtagagaagg
     6541 gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag caaagagttg ctgaaattct
     6601 gccgtaagtt cactgttccg ctacgcgctg ccttgcgcga tgctggggtg ctggcgaact
     6661 atgaaacgcc gaagcgtccg gttgtgcatg tattctttat tgcaccagga tgctgctata
     6721 ccggttactc atacagcaac aataattcgc cgttctatat gggcattccg cgcctgaaat
     6781 ttccggcaga ggcgccgagt cgttccacgc tcaaactgga agaggcattt catgtgttta
     6841 ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat gtgggcggtg gatttaggcg
     6901 cttgccctgg cggctggacc taccaactgg tgaagcgcaa catgtgggtt tattccgtcg
     6961 acaacggccc gatggcgcaa agtctgatgg ataccgggca ggtgacgtgg ctgcgggaag
     7021 acggtttcaa attccgtccg acacgcagca atatctcctg gatggtatgc gatatggttg
     7081 aaaaaccggc taaagttgcg gcattgatgg cgcagtggct ggttaatggc tggtgccgtg
     7141 aaaccatttt caatctcaaa ctgccgatga aaaaacgcta cgaagaagta tcacacaatc
     7201 tggcgtatat tcaggcacag cttgatgaac atggcataaa tgctcagact caggcgcggc
     7261 agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg ccgcatctgg gctgcggtgg
     7321 gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg tcgtctccgg cctgctaccc
     7381 tttccgtcag gctgttacca aagaagttgc aacctggcgc tcatcagaaa taatgtgcgt
     7441 tacctcatcc agatgcccga tgcgggcttc ccctgaacga ttaaatttac ttttatcaat
     7501 caataacaac gattgcgcgg cacgttttaa tagcatcgat ttgtaatcag cgttgatcgc
     7561 attggagtcc cacagtgcgc cgctgctatc gatcccttca caagaaaaaa taaacagatc
     7621 gatttccagc gatttaagtt gggaaatcag cgagggattg acgtaacagc catatttgcg
     7681 ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg cgtttaccga gttcatggca
     7741 aatcggatgg ctattggtga agacctgaat gttgatgtca ggcaactggc gtgccagata
     7801 ccagcaagtt gaactggcgt ctaaggctat caccatccct tcttcaatcc acgcgagcgc
     7861 ctcgcgcgcg atatctgctt tatgcgcata atggcttttc agcctgatgt gaaagggatc
     7921 gccactgtct tgattttgac ggtggatata tttagcgcgt ccatgattgc gcagaatttt
     7981 accctgcgtc tgtaattcat tgagatcgcg acgaatggtt tctttactga cctttagctg
     8041 ttcagagaga gcttccgtgg tcaggctggt atggttcagc agcaggtcga ctatcgcttg
     8101 ctggcgtgcc gctttcatat ttttcacccc atcattcgat aaccgcccgc gtcatgcggg
     8161 cgtaccggta tgagattacg gtgttacccc tttttttaaa agaatattcc cgtacttcgc
     8221 tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc cgttcataaa acgcaaaacg
     8281 attgatgcgg atgatgtcag gacacggggc ttgtagtgaa agcgcattac ggtaacgtcg
     8341 ttctacttca ggatcgagag tgtcaccctc taccgccgcc atcatcacca gcagcggggc
     8401 ataactgtcc agctcaaata acgggataat cgcctggagc aagtcgctca ccaacaggcc
     8461 atcagcgcgg atcacctgcg gttccatcga atgggcggga aagtgagcat cggaaaaaat
     8521 aatttcatct ccatgtccca tctctgccag cactttcaat agttcgggag aaattaacgg
     8581 cgaaattgtt ttcagcattt cacacttcct ctataaattc aggttcagtt tgcgggtaga
     8641 aataacggta ctgataatga atctgtgcac gggcttcttc cgggctgtta aattccccta
     8701 cgccatacca accgaacagc gcagctcctg cgacggtcgt ttcggcatcg tcgagaactt
     8761 ttaccggaat atcaagcata ttggctttaa tctgattcca caatgtgttg cgacttcctc
     8821 caccgactaa caataattca taggctttaa agtgcccgat tttttccagc atctgtagat
     8881 tgcgctgtaa ttgcgcagtt aacccttcca gcgccgcgcg atagaaatgc ccccgcgtgg
     8941 tattaagcgt cactccttgc cagccagcgt tctgacacga caataaatca cactgcattt
     9001 tcacaccatc cgcgccaggt gcgatcagac gagcttcttc aatcaacatt tgccagggtg
     9061 tttcagccgt ccagaacagt tttctcaccc attccagcac gccggatgcc agccattgca
     9121 tacctgggtt atacaaccat gcctggctat ctagttcgca ggtggaaccg gcgtactgac
     9181 ttaacagcga agtatcaacc taggcgctgc gaaccattaa aatttcccat gtaccggagg
     9241 agagcacggg ttcattttgt tcggcaccag cgccaaaaag ggcgaactgg gtatcgtgac
     9301 ctgcggaaat caccggtatg ccgacgggta agccgagcat tgctgcggcg ttgttctgta
     9361 gcgtaccaat ctgttcaccc gcttccacca gacgagggaa gagtcggcgt ggaataccgg
     9421 tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat atccagcatc tggctggttc
     9481 cggccatcgt gatatcagta gtgaattcgc tggttaaacg gtggttaatc agcgacgaaa
     9541 tgaagagcca ggcgtgcgcg cgttccagca gttgtggatg attttctttc aaccacacca
     9601 acttatataa cgtattgaaa ctaaaggctc cgacgccaga aatagcctgc aaccgctgtg
     9661 cggagattaa ccgttcaata ttgtccataa ccgctgctgt tcgcggacat ttccagctaa
     9721 tgatcggata gagcagattg ccttgcttat ctaccagagc gccatccaca ctaaaggtgg
     9781 tgacggcgat accgcggatg tggcattcag tcagttcact attgatttgc cgacagcaat
     9841 cagcaaagcg ttgcaaaatg gcagtctaaa gaccactggt gccaggtgtt gttttccatc
     9901 gcgatatcgc tggcattagg cgttgaggcg cgggcaacaa tttttccctg ccgattaacc
     9961 gcgatggccc tgacattggt cgcgccacag tcgagtacca ggataacttc ttgtttcata
    10021 atcgctcctg caatatagcc ggataacatt gtttatccgg ctaaccactc ttgcttgttc
    10081 aggcccggta gccggagcga ccgggcatca catcagggtg taatgtatta acgcttgtac
    10141 aacggaccgt agttctggca agcgcggtaa tcctggcctt caatatccat gccgtgcgca
    10201 gcccaggcag aaggacgata cactttggtc tcttcaacgt tgtgcataca taccgggata
    10261 cgcagcatgg aggcaagggt gataaagtct gcgccaacgt ggccgatggt cagaactccg
    10321 tggttggcac cccagttcgc cattaccgaa tacacatccg taaacggccc tttaccagtg
    10381 aggcgcggtg caaaccaggt ggttggccag gttgagttgg tgcgtttgtt gaggatgtca
    10441 tgcacatcct tcggcaattc cacgctccag ccttccgcga tttgcagtac cggtcccagg
    10501 cctttgatga tgttgacacg agtcatggtg aacgggacgc cgccttcggt aaggaagcgg
    10561 gaagagtaac cgccgccacg gaagtattcg tggatcgccg ggcaccattt ggtagcggcg
    10621 aggcaagcgt cagcctcttg ctgagagatt tcccagtgcg gcttcatcgt cgggttacct
    10681 tcgctgtcgc gttgtttaca ggaaccgtcc agcgcagcag aacctgagtt gatcaaatgg
    10741 atgatgccgt gttctgccag tccgtccagt ttatgccccg ttacacgctc aattgcttct
    10801 ggtgaccagt aggtacgcac atcggcaaat acctgagcgg tgccggtgag ctggtgaccc
    10861 attagcattg ccacgccgtt aagactgtcg ttttcggtcg ccacgacaaa gggttcgcgc
    10921 acgccattcc agtcaaatga actgttgagg atcgcttcgg cggtgtcacc attgggatat
    10981 tgatcggtcc agtgacgttg cccctggaag cccgcagcga tggcgttgta gccaagtgat
    11041 tcttccacgc gaccaatatc ggccagtttg ctgttgcctt gcatcatgtc gcggatacac
    11101 atcgccatca gtaaactttc gcgcagaact gcgcggcttt gctcggcatt gcgttgatac
    11161 tgtttgttat tttcatcttc gtcatagcgg aagtttttat cagcccaggc cagtgccatt
    11221 tccagttcgg cttcgtcgta aatcttctga tcgatacggc gacgcagttc ggtcatatcc
    11281 accgcctgga ctttcattcc cagccaggat tcaaagaagt tgtgatcaac aatagaaccg
    11341 gcgataccca tcgaaacgcc gcccagcgac agatagcttt tacctttcat gctggcgacg
    11401 gccaaaccgg cgcgggcaaa gcgcagcagt ttttcttcaa catcggcagg aatcgatgtg
    11461 tcatcggcat cctgaacgtc atgaccgtaa atggagaatg ccgggatgcc tttctggctg
    11521 tgagctgcca gagccgctgc caggtaaaca gcgccggggc gttcagtgcc gttaaagccc
    11581 caaatggctt tcgggcgggt tggatccatg tcgatggttt cactgccata gcaccagcaa
    11641 ggcgttaccg taatggtgag gcctacattc tggctgctga atttttcttc gcaagcagcg
    11701 gcttcagcca tacccgcgat acaggtatcg gaaatgacac actcgacggc agctccgcag
    11761 gcatggcgca gtttctcggt cagcagtgcg gccgtagctt tcgccatatt cattgtttgt
    11821 tcttcaagcg actcacgaac acccatgcga cgaccgtcaa taaccgggcg gataccaatt
    11881 ttcggtaagc tgattttttt cattacagat tcctcacttt attcggaaaa tatgttcagt
    11941 tagttgccgt ttgagaacgg aaacgggcaa agataaagat gaccgcgaag cagagtgcgg
    12001 ggatcagttc agcagtgggg atgttgcccg ccgcgtcact gacaaaaccc atgaccggag
    12061 tgacaatacc gccgccaata atggtcataa cgatgaagga cgaaccgtat ttggtgtcct
    12121 ggccgagatt cttaatgccc agcgagaaga ttgttgggta ctgaatcgac ataaaggcgc
    12181 tgcataaagt cagggctatt aagcccacat gaccgccagc gaaggctgag atcaggcaca
    12241 gtgccatagc gattaatgcg taggcggcca ggactttgtg tggtgcgaag cgactgatga
    12301 gccaggtacc ggtgaaacga ccaataaaga agcacaccat ggttccggtt aaatagttag
    12361 cggcaaagcc tgcagtcata cctggaattt cttctacagc gtagcgaatc aaatagctcc
    12421 agcaggccgt ttgtgcgccg acatagcaga attgcgctaa taccgcccag cgccagtgac
    12481 gaatacgcgc cagacgagaa agcgatgcgg agaacgatcc ttgtttggcg tcactgtgat
    12541 tatcactctg caatgccggg aatttcgtca gcatgatcag cagggcgacc agtaacacga
    12601 tagccacgat gatcatataa ggtgtctgta ccgataatac caggctgtgt ttatacgcac
    12661 tcaattgctc tggagacatt ttatcgagaa cgtcttgcga ttgatgtggc acgttagaca
    12721 aaataagact ttgcccaaag acaaccgcga taattgcgcc aaacgagtta aatgtttgcg
    12781 caagatttaa gcggaagtga ctactgcttt ccggccctaa taccgtaaca aaagggtttg
    12841 cggcagtttc cagacaacct aatccggctg caataataaa taggccaact aaaaataagg
    12901 tgtagttcat tatttctgca gcgggccaga ataatgcagc gcccaaggca tataaaaata
    12961 atccggtaat aatccctgct ttataactga gttttttcat caatatccca gcagggattg
    13021 ggataatgaa ataaccaaag taaaaggccg attggatcag gccagcctgg aaatttgtca
    13081 gcgtaaaagc ctgctggaat tgaggtaata aaatgtcgtt aaggttattg gctaccgccc
    13141 aaagaaaaaa cagtgagcac agcagcgcga atggaatgat gtaacttctg ctttgccctg
    13201 catctttatc taccgcacgg taactctgcg tttgtattga tgtgtttccc atagcatcct
    13261 cttaggttca gaagcttaag cacgtaatcg gttatgtttt caaaagtgca aaaccagcga
    13321 gttcgggctt catatcggtg aatatttgcc tgtctgaaat gaactcatgg attcattttc
    13381 aaataaaaaa cgctctgtaa tatgacggcg gtcacactta ttagctggaa ggtgacttta
    13441 tgtgactacc atcactttaa taatcgaaat ttcatgcccg tttcagaaaa taaatcataa
    13501 aaaacacaaa ccagatgacc gtattaattg tttgtgccga aaacgggcat ttctggcgag
    13561 gggaaatgga aaaatgaccg ttttgataaa tatttttaag agcggtcatt tttaacgggg
    13621 ttataaaaag agaacttgca gttatttact gtgattagtt gagctaggtc acaaaatata
    13681 agcttgtgaa tttctttctt gttaatcagg aagaagatgg tgggtaatta aacggctaat
    13741 tcaatagtgt gaaaggaaca acattattgc cctgttttga atcagagaga ggtagctaat
    13801 ggaacgaaat aaacttgctc gtcagattat tgacacttgc ctggaaatga cccgcctggg
    13861 actgaaccag gggacagcgg ggaacgtcag tgtacgttat caggatggga tgctgattac
    13921 gcctacaggc attccatatg aaaaactgac ggagtcgcat attgtcttta ttgatggcaa
    13981 cggtaaacat gaggaaggaa agctcccctc aagcgaatgg cgtttccata tggcagccta
    14041 tcaaagcaga ccggatgcca acgcggttgt tcacaatcat gccgttcatt gcacggcagt
    14101 ttccattctt aaccgatcga tccccgctat tcactacatg attgcggcgg ctggcggtaa
    14161 ttctattcct tgcgcgcctt atgcgacctt tggaacacgc gaactttctg aacatgttgc
    14221 gctggctctc aaaaatcgta aggcaacttt gttacaacat catgggctta tcgcttgtga
    14281 ggtgaatctg gaaaaagcgt tatggctggc gcatgaagtt gaagtgctgg cgcaacttta
    14341 cctgacgacc ctggcgatta cggacccggt gccagtgctg agcgatgaag agattgccgt
    14401 agtgctggag aaattcaaaa cctatgggtt acggattgaa gagtaatttc gtaaagcaac
    14461 aaggagaagg atgatggcta acagaatgat tctgaacgaa acggcatggt ttggtcgggg
    14521 tgctgttggg gctttaaccg atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt
    14581 caccgataaa acgctggtgc aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc
    14641 tgcagggctg gcatgggcga tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt
    14701 caaagaaggg ctcggtgtat tccagaatag cggcgcggat tacctgatcg ctattggtgg
    14761 tggttctcca caggatactt gtaaagcaat tggcattatc agcaacaacc cggagtttgc
    14821 cgatgtgcgt agcctggaag ggctttcccc gaccaataaa cccagtgtac cgattctggc
    14881 aatccccacc acagcaggta ctgcggcaga agtgaccatt aactacgtga tcactgacga
    14941 agaaaaacgg cgcaagtttg tttgcgttga tccgcatgat atcccgcagg tggcgtttat
    15001 tgatgctgac atgatggatg gtatgcctcc ggcgctgaaa gccgcgacgg gtgttgatgc
    15061 gctcactcat gctattgagg ggtatattac ccgtggcgcg tgggcactaa ccgatgcact
    15121 gcacattaaa gcgattgaaa tcattgctgg ggcgctgcga ggatcggttg ctggtgataa
    15181 ggatgccgga gaagaaatgg cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt
    15241 tgggttaggg ttggtgcatg gtatggcgca tccactgggc gcgttttata acactccaca
    15301 cggtgttgcg aacgccatcc tgttaccgca tgtcatgcgc tataacgctg actttaccgg
    15361 tgagaagtac cgcgatatcg cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga
    15421 aggggcgcgt aatgccgctg ttgaagcggt gtttgccctc aaccgtgatg tcggtattcc
    15481 gccacatttg cgtgatgttg gtgtacgcaa ggaagacatt tcggcactgg ctcaggcggc
    15541 actggatgat gtttgtaccg gtggcaaccc gcgtgaagca acgcttgagg atattgtaga
    15601 gctttaccat accgcctggt aaatgcgctg atgtgataat gccggatacg atttgcggcg
    15661 tatccggctc gccgttaccg taccaaccgc aattgctgta aattgccgtc gatatgcaaa
    15721 tcggtttgta agcgggcaat atcgcggcac agaaacgcca tctctttatg ggtttctaat
    15781 tttttgcgcc acttttcggc aaccgcatcc agattctcat atatcccttc cagactctga
    15841 aactcgacca gcagctgcgt ggcgcttttt ggtccgattc ccgcaacacc cggtaccttt
    15901 gaactgctga tccccgcaag tccccagtaa tcgggcaact gctgcggttg aacgccaaat
    15961 tctttatcga taaatggcgc atccagccaa cgtttctgga agtaatcacg aatacgtaat
    16021 gtcggtgaaa gtaactgaca gtagccttta tctgtcgaaa caatcgttgc ctgatgcccg
    16081 gcctgtgtca ctttgaccgc cagtgtggcg gctaagtcat cggcttcgtt gccgctggct
    16141 gaccagcacg ggacgccgcg ttgctcaaag gcggcgcgta atgcaggcat ctcgtcgtgc
    16201 aactcttccg gcattggcgg tcgatccgct ttgtaatctg gtaaacgctg atgacgccag
    16261 ccgctactgc ggttttcatc atcaaaaacg gcgaccgcgt gggttggctg gctgtgcata
    16321 atgagctgat cgagcgcatg ctggcaggtt tcgacacagg gcgaaccctg aacggcatga
    16381 atgcgacgga taagattcag tgcatcgaca ataagcaaat gaacagccac gataaccccc
    16441 ttaaagatca ggcgataagg gtaacattcg atgccggaac aggctatggc tggttgagaa
    16501 aacaggaaga ggcttcgcaa aacgaggcct ctgggagaga ttaatcgcag gcaacgatct
    16561 tcatcgccag gccgccgcga gaggtttcgc ggtacttggc gttcatgtct ttacctgttt
    16621 cgtacatggt ttcgataact ttatcgaggc agacgcgcgg ttcgctggta cggcgcagtg
    16681 ccatacgcgc ggcgttcacc gctttcactg ctgcaatggc gttacgctca atgcacggta
    16741 cctgaacctg ccccgcgacc gggtcacaag tcagaccgag gttgtgctcc atcgcgattt
    16801 ccgctgcgat gcaaacctgc gccgggcttg cgcctaacag ttctgccaga ccagctgccg
    16861 ccatagagca cgccacgcca acttcaccct ggcaacccac ttcggcacca gaaatcgacg
    16921 cgttcatctt ataaagagaa ccaatggcgc tggctaccag caggtaacga gccagtgagt
    16981 tagcgttcac ttcgcggata aacttatcgt agtacgccag cacagccggg ataatcccgc
    17041 acgcaccgtt agtcggcgca gtcaccacgc gaccgccagc agcgttctct tcgttcactg
    17101 ccagtgcaaa catgttgatc cagtcaacaa ccgccatcgg gtcagtggtg gttttatcca
    17161 ggctgaccag catccggcgt agtgccgcag cacggcgtgg aacgcgcagt ttgccaggca
    17221 acacgccttc ggtggaaata ccgcgctcaa taccgccacg catgacttcc cagacgttcg
    17281 ccaggtgctg ttccagctct tctttactgt gcagcgccag ctcgtttttc atcatcaggc
    17341 cagagagtga cagcccggtt tcctgacaat gtttttgcag atcggctgct gaactgtacg
    17401 gataaggaac ttcaaccggt gcgctatcct gctggccaaa atgctcttca tcaacgataa
    17461 agccaccgcc aatagagtag taagtctggc tgtaaacgac tttatcgccc gccagcgcgg
    17521 taatgcgcat accgttttca tgcagagaaa ggttatctgc atggaagttc atgcactgat
    17581 caaccgggaa ctccacttca tgctgaccgt ttgccagcat caggcgacca tgagtattca
    17641 catcctgaat aaaaccgggg atggaatcga tatccacggt atccggcagg ttacccgcca
    17701 ggcccataat aatggcgata tctgtgtggt ggcctttacc agtcagagag agcgagccgt
    17761 acacgtcaac caccacgcgg gtcacgtctt taagcaggtt acgggcaatc agatcgtcgg
    17821 taaattgttt acccgctttc attggtccaa cggtatgaga actggaaggg ccaatgccga
    17881 ttttgaaaat atcgaatacg ctaatcatag gaaatacatc gcgttaaaac ggaggaagcg
    17941 ccgcccgaaa gcggcgcgaa aggacttagc tgaacagaga gtagaagatt gcggagattg
    18001 caatcagacc catcacgaca acgaatacgt tgctgatgtg accgctgtac ttacgcattg
    18061 ccggtacttt ctgaattgcg tacatcggca tcaggaacag gatcatcgcg atgattgggc
    18121 cgcccagggt ttcaatcata cccaggatgc tcgggttcag ggtggcaaca atccaggtcg
    18181 ttaccagcat gaacagcgca gtgatacggt tcagcttgtt gatttcgata gacttacctt
    18241 taccacgcag agatttaatc accataccgt tgaagccttc acgtgcgccc aggtagtggc
    18301 cgaggaagga tttggtgata gcgataatcg cgataatcgg agccatccac gcgataaccg
    18361 gtgcgttaaa gtggttagcc aggtaagaca gaatcgagat gttctgctct ttagccgcag
    18421 ccaggtctgc cggagtcagg ctcagtacgc agctgaagac gaagaacatt acggtcagca
    18481 ccatcatgat gtgtgcgaat gccaggatct tcgagcattt ctgttctgcc atatcgccgt
    18541 actcttcacg cttcgcaacg gcgaaagaag agatgatcgg agagtggttg aacgagaaca
    18601 ccattaccgg aattgccagc cacagggtca tccacagacc gtttccggtt gcagatgcag
    18661 tgtccagaga cagcgtttcc agtgctgcgc cgttccactg cgggatcagg tacagagcca
    18721 gcagcatcag tacgccaaca aacgggaata ccagaatact catcgcttta acgatcatct
    18781 gctcaccaaa gcgaacgatg gtcatcatac ccacgatcag gatcagcgac agaatcgcac
    18841 gcggcggtgg cgtcataccc agctggtgag acatgaagct ttcaacggta ttggtgattg
    18901 ccacgctata aaccagcagg atcgggtaga tagcgaagaa gtagagcagg gtaatcagtt
    18961 tacctgcgcc aataccaaag tgttcttcta caacctcggt gatgtcttcg cccgggtttt
    19021 taccagacag tacgaagcga gtcaggccgc ggtgagcaaa aaacgtcatc gggaacgcaa
    19081 ggatagccat gatgatcagc gggatcatac cgccaacacc ggcgttgatt ggcaggaaca
    19141 gcacgcccgc gccgattgcc gtgccgtaaa ggcccagcat ccacatggtg tctgtcttgc
    19201 gccaggcact acgagagtct ttcgacgcaa tcgtgctggt ttgagtcgtt tccatctatt
    19261 tctcctggag gaatgcaa
//
LOCUS       DAJTUN010000081        19252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTUN010000081 DAJTUN010000000
VERSION     DAJTUN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.081"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..2072
                     /gene="pbpC"
                     /locus_tag="OO470_002598"
     CDS             <1..2072
                     /gene="pbpC"
                     /locus_tag="OO470_002598"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6251807.1"
                     /translation="NYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLTMQVAR
                     LLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGAASWAYLGK
                     SPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSREQVKESR
                     EEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRS
                     SLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGL
                     IHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAYGPKRFAA
                     KLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLL
                     ERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAIGVNARYV
                     IGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTRGVICWPG
                     GQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQRVAADCPQ
                     AKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKR
                     LPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDDVGQIA
                     TVKFVMQ"
     gene            2222..2653
                     /gene="ndk"
                     /locus_tag="OO470_002599"
     CDS             2222..2653
                     /gene="ndk"
                     /locus_tag="OO470_002599"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6251808.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2803..3957
                     /gene="trmG/rlmN"
                     /locus_tag="OO470_002600"
     CDS             2803..3957
                     /gene="trmG/rlmN"
                     /locus_tag="OO470_002600"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6251809.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4242..5255
                     /gene="rodZ"
                     /locus_tag="OO470_002601"
     CDS             4242..5255
                     /gene="rodZ"
                     /locus_tag="OO470_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6251810.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5282..6409
                     /gene="ispG"
                     /locus_tag="OO470_002602"
                     /gene_synonym="gcpE"
     CDS             5282..6409
                     /gene="ispG"
                     /locus_tag="OO470_002602"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6251811.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6512..7786
                     /gene="hisS"
                     /locus_tag="OO470_002603"
     CDS             6512..7786
                     /gene="hisS"
                     /locus_tag="OO470_002603"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6251812.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7804..8424
                     /gene="yfgM"
                     /locus_tag="OO470_002604"
     CDS             7804..8424
                     /gene="yfgM"
                     /locus_tag="OO470_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6251813.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8435..9613
                     /gene="bamB"
                     /locus_tag="OO470_002605"
     CDS             8435..9613
                     /gene="bamB"
                     /locus_tag="OO470_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6251814.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9731..11203
                     /gene="der"
                     /locus_tag="OO470_002606"
     CDS             9731..11203
                     /gene="der"
                     /locus_tag="OO470_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6251815.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11273..11488
                     /locus_tag="OO470_002607"
     CDS             11273..11488
                     /locus_tag="OO470_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6251816.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11485..12855)
                     /gene="xseA"
                     /locus_tag="OO470_002608"
     CDS             complement(11485..12855)
                     /gene="xseA"
                     /locus_tag="OO470_002608"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6251817.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13017..14483
                     /gene="guaB"
                     /locus_tag="OO470_002609"
     CDS             13017..14483
                     /gene="guaB"
                     /locus_tag="OO470_002609"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6251818.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14552..16129
                     /gene="guaA"
                     /locus_tag="OO470_002610"
     CDS             14552..16129
                     /gene="guaA"
                     /locus_tag="OO470_002610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6251819.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16224..16763)
                     /locus_tag="OO470_002611"
     CDS             complement(16224..16763)
                     /locus_tag="OO470_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6251820.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16779..17297)
                     /locus_tag="OO470_002612"
     CDS             complement(16779..17297)
                     /locus_tag="OO470_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6251821.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17608..17799)
                     /locus_tag="OO470_002613"
     CDS             complement(17608..17799)
                     /locus_tag="OO470_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6251822.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17817..17969
                     /locus_tag="OO470_002614"
     CDS             17817..17969
                     /locus_tag="OO470_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251823.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18151..>19252
                     /gene="pdeF"
                     /locus_tag="OO470_002615"
     CDS             18151..>19252
                     /gene="pdeF"
                     /locus_tag="OO470_002615"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6251824.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEML"
BASE COUNT         4530 a         4688 c         5236 g         4798 t
ORIGIN      
        1 tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc tcggtggcgc
       61 gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc acgctcacta
      121 tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa attctccagc
      181 tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg atcttgtatc
      241 ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tgcggcaagt tgggcttatc
      301 tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg gtgttaccgc
      361 aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc gcgcgtaata
      421 aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta aaagagtcaa
      481 gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg ctgttttcgc
      541 gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat gccggtcttc
      601 aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg cgcagctcac
      661 tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg ggatcggttg
      721 atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc cgatcgccag
      781 gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg atccacccgg
      841 catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt aactttgata
      901 gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg aacttacctg
      961 ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta cgcaatgttg
     1021 gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt ctcggcggcg
     1081 ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc cacggcaagg
     1141 caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta atgtcgtcgg
     1201 gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg ccggatagtg
     1261 ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc tatcgtgacg
     1321 cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc agacctgacg
     1381 gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattgttg aatcaggtca
     1441 ataatatctt actgtcgcgc agtgcgaatc tgccggaaga cccgcgcccg aactcagtca
     1501 ctcgtggcgt tatctgctgg ccgggcggac agtctttgcc ggaaggtgac ggtaactgtc
     1561 gccgccgcct ggcaacctgg ctgctggatg gcagccagcc gccaacgctg ttactgtcag
     1621 agcaggaagg gattaatggc attcgcttcc ccatctggct ggatgacaaa gggcagcgcg
     1681 tcgccgctga ctgcccgcag gcgaagcaag aaatggtaaa tgtctggccg ctaccgctgg
     1741 aaccctggct gcccgcatca gaacgtcgcg ctgtgcgctt accaccggcc tcgacaattt
     1801 gcccgccata cggtcacgat gcccagctcc ctctgcagct gacaggcgtt cgcgatggcg
     1861 cgattattaa acgtttaccg ggcgcagcgg aagcgacttt gccgttgcaa tcaagtggag
     1921 gggcaggtga acgctggtgg tttctgaatg gcgaaccgtt aactgaacgc gggcgcaacg
     1981 tgaccctgca tttgacggat aaaggtgatt atcaattgct ggtgatggat gatgtggggc
     2041 aaatcgcgac agtgaaattt gtcatgcaat agtctgtttt gtccctgatt gttgctaaaa
     2101 ctcattttat tttaaaaaaa atgttacctt catcaatagt caacggccct gttgctcatt
     2161 ataatccgcg ccatctcgta cgctggtaca gacaacaaca gaacaattta cagaggtaaa
     2221 aatggctatt gaacgtactt tttccatcat caaaccgaac gcggtagcaa aaaacgtcat
     2281 tggtaatatc tttgcgcgct ttgaagctgc agggttcaaa attgtcggca ccaaaatgct
     2341 gcatctgacc gttgaacagg cacgtggctt ttatgctgaa cacgatggaa aaccgttctt
     2401 tgatggtctg gttgaattca tgacctctgg cccgatcgtg gtttccgtgc tggaaggtga
     2461 aaacgccgtt cagcgtcacc gcgatctgct gggcgcgacc aatccggcaa acgcactggc
     2521 tggtactctg cgcgctgatt acgctgacag cctgaccgaa aacggtaccc acggttctga
     2581 ttccgtcgaa tctgccgctc gcgaaatcgc ttatttcttc ggcgaaggcg aagtgtgccc
     2641 gcgcacctgt taataatttc gtaaatgccg cgtgcaaacg tggcatccgt gcgccagaat
     2701 ttgtacaatg cagcgccccc ggacgagcag ccgctcaccg gggcgtttct tttttcaacc
     2761 ctccaggggc cataacgtgt aataacgagg ccggaatagc atatgtctga acaattagtc
     2821 acacctgaaa acgtcaccac gaaagatgga aaaatcaacc tgctggatct caaccgtcag
     2881 cagatgcggg agttttttaa agatttaggt gaaaaaacct tccgcgccga tcaggtgatg
     2941 aagtggatgt atcactattg ctgcgacaac tttgatgaga tgaccgacat caacaaagtg
     3001 ttgcgcggca aactgaaaga ggtggcggaa atccgcgcac cggaagtggt tgaagaacag
     3061 cgttcatccg acggcaccat taaatgggcg atcgccgttg gcgatcagcg cgtcgaaacg
     3121 gtgtatatcc cggaagacga ccgtgccacg ctctgcgtct cttcgcaggt ggggtgtgcg
     3181 ctggagtgta aattctgttc caccgcccag cagggcttta accgcaacct gcgggtatcg
     3241 gaaattattg gccaggtgtg gcgtgcggcg aaaatcgtcg gcgcagcaaa agtcaccggt
     3301 cagcgcccaa tcactaacgt cgtgatgatg ggcatgggcg agccgctgct caacctgaac
     3361 aacgtcgttc cggcgatgga aatcatgctt gatgatttcg gttttggcct gtctaaacgt
     3421 cgcgtgacgc tttccacttc cggtgtagtt ccggcgctgg ataaactggg cgatatgatc
     3481 gacgttgcac tggcaatttc cctgcacgcg ccgaacgacg aaattcgtga cgaaattgtg
     3541 ccgatcaaca aaaagtacaa tatcgaaacg ttccttgctg cggtgcgccg ttatctggag
     3601 aaatccaacg ccaatcaggg ccgagtcact attgaatacg tgatgcttga ccacgttaac
     3661 gacggcactg aacacgcgca ccaactggcg gaactgctga aagatacgcc gtgtaagatc
     3721 aacctgatcc cgtggaaccc gttcccggac gcgccgtatg gacgcagttc gaacagccgt
     3781 atcgaccgtt tctcaaaagt gctgatgagc tacggtttca ccactattgt gcgtaaaacg
     3841 cgtggtgatg atatcgatgc tgcctgtggt cagttggcgg gcgatgttat cgaccgtacg
     3901 aaacgtactc tgcgtaaacg gatgcagggt gaagccatcg atattaaagc ggtctgatag
     3961 ccattacgcc acggttactt tatgtaccgt ggcgtaatct tttatgaatg aagtattaag
     4021 ttgcaattgt ctggtcaata ttaacggtgc gtttttgctg actttaaggc agtatgtaac
     4081 gatgcaacag taagttagcc tcagtgaatg tgggctttgt cacgagcaca cagacggtct
     4141 tatactgtat gataacggtt aacttaacgg atgtttcgcg gtgtgggtga gcatgatggt
     4201 tcaccggcat ctcaattctc atttaaacgt acctgcagcg aatgaatact gaagccacgc
     4261 acgaccaaaa tgaagcactt actaccggcg ctcgcctgcg taatgctcgc gaacaactag
     4321 gacttagtca gcaggccgtt gccgagcgac tttgcctgaa ggtttccacg gtacgcgaca
     4381 ttgaagaaga taaggcaccc gccgatcttg cttcaacatt cctgcgcgga tatatccgct
     4441 cttatgcgcg tctggtacat attccagaag aagaactgct gccagggctg gaaaagcagg
     4501 ctccacttcg ggctgcaaaa gttgcgccaa tgcagagttt ttccctcggt aaacgccgca
     4561 aaaaacgcga cggctggcta atgaccttca cctggctggt gttgtttgtg gttatcggcc
     4621 tgagcggtgt ctggtggtgg caagaccaca aagctcagca ggaagagatc accactatgg
     4681 ccgatcaatc ttcggcggaa ctgagcagta atagcgagca ggggcagagt gttccgttaa
     4741 atacgtcgac aactacagac ccggctacaa cgtccacgcc gccagcgtct gtggatacta
     4801 ccgcaaccaa cacgcaaaca cctgccgtaa ctgcgccagc accagctgtt gatccgcaac
     4861 agaatgcggt tgtttcgcct tcgcaggcaa atgttgatac cgccgcgacc ccggtaccaa
     4921 cggcagcaac aacgccagat ggtgctgcgc cgttgccaac cgatcaggct ggcgtgacca
     4981 cgccggcggc tgatccgaat gcgctggtga tgaactttac tgccgattgc tggctggagg
     5041 tcactgatgc taccggtaaa aaattgttta gcgggatgca gcgtaaagac ggtaatttga
     5101 atttaaccgg ccaggcaccg tataaactga aaattggtgc gccagccgca gtacagatcc
     5161 agtatcaagg gaaacctgtc gatctgagtc gttttatcag aactaaccag gttgcgcgtc
     5221 tgaccctcaa tgccgaacaa tcaccggcgc agtaacagac gggtaacgcg ggagattttt
     5281 catgcataac caggctccaa ttcaacgtag aaaatcaaca cgtatttacg ttgggaatgt
     5341 gccgattggc gatggtgctc ccatcgccgt acagtccatg accaatacgc gtacgacaga
     5401 tgtcgaagca acggtcaatc aaatcaaggc gctggaacgc gttggcgctg atatcgtccg
     5461 tgtctccgta ccgacgatgg acgcggcaga agcgttcaaa ctcatcaaac agcgggttaa
     5521 cgtgccgctg gtggctgaca tccacttcga ctatcgcatt gcgctgaaag tagcggaata
     5581 cggcgtcgat tgtctgcgta ttaaccctgg taatatcggt aatgaagagc gtattcgcat
     5641 ggtggttgac tgtgcgcgcg ataaaaacat tccgatccgt attggcgtta acgccggatc
     5701 gctggaaaaa gatctgcaag aaaagtatgg cgaaccgacg ccgcaggcgt tgctggaatc
     5761 cgccatgcgt catgttgatc atctcgatcg cctgaacttc gatcagttca aagtcagcgt
     5821 gaaagcgtct gacgtcttcc tcgctgttga gtcttatcgt ttgctggcaa aacagatcga
     5881 tcagccgctg catctgggga tcaccgaagc cggtggtgca cgcagcgggg cagtaaaatc
     5941 cgccattggt ttaggtctgc tgctgtcgga aggcatcggc gacacgctgc gtgtatcgct
     6001 ggcggccgat ccggtcgaag agatcaaagt cggtttcgat attttgaaat cgctgcgtat
     6061 ccgttcgcga gggattaact tcatcgcctg cccgacctgt tcgcgtcagg aatttgatgt
     6121 tatcggtacg gttaacgcgc tggagcaacg cctggaagat atcatcactc cgatggacgt
     6181 ttcgattatc ggctgcgtgg tgaatggccc aggtgaggcg ctggtttcta cactcggcgt
     6241 caccggcggc aacaagaaaa gcggcctcta tgaagatggc gtgcgcaaag accgtctgga
     6301 caacaacgat atgatcgacc agctggaagc acgcattcgt gcgaaagcca gtcagctgga
     6361 cgaagcgcgt cgaattgacg ttcagcaggt tggaaaaata ataacgtgat gggaagcgcc
     6421 tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga agcgttgagg
     6481 gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag ccattcgcgg
     6541 catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag gcacactgaa
     6601 aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag agcagacccc
     6661 gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga tgtacacctt
     6721 tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg gctgtgtacg
     6781 cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt atatcgggcc
     6841 gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc agttgggctg
     6901 cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc tcactgcccg
     6961 ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt ctatcggttc
     7021 gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc agcataaaga
     7081 aaagctggac gaagactgca aacgccgcat gtacactaac ccactgcgcg tgctggattc
     7141 aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg attatctgga
     7201 cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg cggggatcgc
     7261 ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta ccgttttcga
     7321 gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc gttatgacgg
     7381 tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga tgggcctcga
     7441 acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc ctgttgtcga
     7501 tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat tagctgagcg
     7561 tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg gcaactttaa
     7621 gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc tgggtgagtc
     7681 tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc aaacggcagt
     7741 tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa ggagaaggac
     7801 agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg cttttttgct
     7861 gaaaatggca aagcactggc tgttgggggg attttgggcg ttggcgcact gattggctgg
     7921 cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc ctatcaaaat
     7981 gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga aaaatttgct
     8041 gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca gcaatttgtt
     8101 gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc agacacgagc
     8161 gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt acagctcaag
     8221 caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg ggctgccatt
     8281 gttgccgact tgcgtggtga agcattgctg agcaaaggtg ataagcaagg tgcgcgtagt
     8341 gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat gatgcagatg
     8401 aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat tactgctgcc
     8461 aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg aagaagatgt
     8521 ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca cggcgtggag
     8581 cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg cactggcgga
     8641 caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg cggatgatgg
     8701 caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta aagagcctgc
     8761 attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca gcgaaaaggc
     8821 gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta aagtcgcggg
     8881 tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca ccagtaacgg
     8941 tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta acctcgatat
     9001 gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg cggccgtcgt
     9061 gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga tgatttggca
     9121 gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg atgttgacac
     9181 gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta acctgacggc
     9241 gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg tgaatgattt
     9301 catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga tggcgttgac
     9361 cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc tgctgacttc
     9421 tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc tgcactggat
     9481 taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg gtttccagac
     9541 tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa ccgtgtactc
     9601 tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac aggggccgtt
     9661 ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt ataaaaatga
     9721 ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag gaaaatccac
     9781 gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc cgggtctgac
     9841 tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct gtattgatac
     9901 cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac agtcgctgct
     9961 ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg gcctgatgcc
    10021 ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct tcctggtggt
    10081 aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact cgcttggttt
    10141 aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc tgctggagca
    10201 tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg aagacgctga
    10261 atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag acgacttcga
    10321 cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag gtaagtctac
    10381 actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc ctggcacgac
    10441 gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc tcattgacac
    10501 cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct ccgtaatcaa
    10561 aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg cgcgcgaagg
    10621 tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc gctcacttgt
    10681 cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg tgaaagaaac
    10741 gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct ctgccttgca
    10801 cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca gctccacccg
    10861 tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag atcaccaacc
    10921 gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg gttataaccc
    10981 gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct acaagcgcta
    11041 cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc gtattcagtt
    11101 caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa cccagatgcg
    11161 taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg taagtatttt
    11221 atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc atatggaact
    11281 gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc gttgcccatc
    11341 ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac cgttgcaggt
    11401 gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct tgatatcgaa
    11461 aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt ttaccggctg
    11521 gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta gcatttcacc
    11581 cgttttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag taacgctgta
    11641 tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg cattaccgaa
    11701 acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat attccagttg
    11761 ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat tcagccgctg
    11821 tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg caaagctcat
    11881 ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct gcggatgctg
    11941 ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgtgta cggttggcga gataataatc
    12001 catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact cttgctgatt
    12061 acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg caacaaaatc
    12121 ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc ggctggcaaa
    12181 aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg aaccgccgcc
    12241 gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa tggcgcgaac
    12301 aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca ccggtagaga
    12361 aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg tttttgaggt
    12421 gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct gatcgaacaa
    12481 accttcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca gcccttcacc
    12541 ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt agagcgtaat
    12601 attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc ggcggttgct
    12661 gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa agtaccagtg
    12721 accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct gtcccatctc
    12781 atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa ttgcagggga
    12841 ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt cagtcgatag
    12901 taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg caatcggtta
    12961 cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata ttgcccatgc
    13021 tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct gctcattcta
    13081 ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt cgtctgaata
    13141 tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct attgctctgg
    13201 ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc caggcagaag
    13261 aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag actgttctgc
    13321 caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt gcgggctatc
    13381 cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac gtgcgttttg
    13441 ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt ctggtcaccg
    13501 tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa cgcgttgaaa
    13561 aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg aaagacttcc
    13621 agaaagcgga acgtaaaccg aacgcctgta aagacgagca aggccgtctg cgtgttggtg
    13681 ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt gccgctggcg
    13741 ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg cagcgtatcc
    13801 gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg gcaacagctg
    13861 caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt atcggccctg
    13921 gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc actgccgttg
    13981 ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat ggtggtattc
    14041 gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg atggtaggtt
    14101 ccatgctggc gggtactgaa gaatctccgg gtgaaatcga actctaccag ggccgttctt
    14161 ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc tctgaccgtt
    14221 atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa ggtcgcgtag
    14281 cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg cgctcctgta
    14341 tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag tttgtacgta
    14401 tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact aaagagtccc
    14461 cgaactaccg tctgggctcc tgattctctt cgcccgattt catgtcgggc gatttatatt
    14521 atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata agcatcgcat
    14581 cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg tgcgtgagct
    14641 gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc gtgacttcaa
    14701 tccaagcgga attattcttt ccggcggccc ggaaagtact actgaagaaa acagtccgcg
    14761 tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct atggcatgca
    14821 gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg aatttggcta
    14881 cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag atgcgctgac
    14941 cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag ttaccgctat
    15001 cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca ttatggctaa
    15061 cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca cccgccaggg
    15121 tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc tgtggacgcc
    15181 agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg acgataaagt
    15241 catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc tgcaccgtgc
    15301 tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc tcaacgaagc
    15361 agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc acgtaccggc
    15421 agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa aacgtaaaat
    15481 catcgggcgc gttttcgttg aagtattcga tgaagaagcg ctgaaactgg aagacgtgaa
    15541 gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt ctgcaaccgg
    15601 taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag agatgaagat
    15661 gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga ttggtctgga
    15721 gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag gccttggcgt
    15781 tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg ctgacgccat
    15841 cttcattgaa gaactgcgta aagcggacct gtacgacaaa gtcagccagg cgttcaccgt
    15901 cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg actgggttgt
    15961 ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc atctgccgta
    16021 cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta tttcccgcgt
    16081 ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat tttgatccct
    16141 gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt caagaatcat
    16201 aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt ctcttctgct
    16261 ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt tgcgtcagct
    16321 tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc gataaattcg
    16381 ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt ctgaatctct
    16441 aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt ggcttcggcg
    16501 tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct tttgttcgcg
    16561 gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt gcgctccgcg
    16621 acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc ttctgcgaca
    16681 gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga ggctaagatg
    16741 gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc aggacatttg
    16801 gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc taaacctgtc
    16861 gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc ttccttatat
    16921 gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc tgccgcgcct
    16981 acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga accgacgttg
    17041 tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg tgcgttccgt
    17101 ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat attaacggtt
    17161 ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc atgatcgtca
    17221 gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg cagaagacat
    17281 tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga catttaagat
    17341 catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt tattaaaatt
    17401 cacgcaggga ataatttata aaaatatatc attacaacat atatattaat tttatattta
    17461 tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt aagatgaaag
    17521 aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa gtgccgttat
    17581 gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga cggagagtgt
    17641 ggattgctga gcttcttttt tgctctgatg gtgctgccag gcaccgacgg acgagtagat
    17701 aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca tgcgactgtt
    17761 aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct tgaagtatgt
    17821 cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc aattcttcgt
    17881 tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat ttaattattt
    17941 acaatgatga tgtaataatg atgaattaac atgataaacg taataattca ttaatcaggg
    18001 ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat caatgctctc
    18061 tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa tacacctcct
    18121 tcaggatgtg ggggattctg ctgagcatct atgaaactga atgcaactta tataaaaata
    18181 cgtgataaat ggtgggggct tccgctgttc ctgccttctt taatcttgcc cattttcgcc
    18241 cacattaata ctttcgcgca tatttcttcc ggtgaggttt ttctctttta tctgcctttg
    18301 gcactgatga tcagcatgat gatgtttttc agctgggcgg cattgccagg gatcacctta
    18361 gggatttttg tccgcaaata tgcagagctg ggtttttacg aaacgctctc attaacggct
    18421 aattttatta tcattatcat tctctgttgg ggcggttaca gggtctttac tccccggcgt
    18481 aacaacgttt cacatggtga tacccgttta atttcccagc gtatattctg gcagatagtg
    18541 tttcctgcaa cgctgtttct gatacttttc cagtttgctg catttgtagg attactggcg
    18601 agcagagaaa atctggtcgg tgtcatgcct tttaacctcg ggactttaat caattatcag
    18661 gccttactgg tgggtaatct gatcggtgtc ccgctgtgct acttcatcat tcgggtagtg
    18721 cgaaatccat tttatttacg tagctattat tcgcaattaa aacagcaggt tgatgccaaa
    18781 gtcaccaaaa aagagttcgc gctctggcta ctggcattag gtgctttatt gttgctgtta
    18841 tgcatgccgt taaatgaaaa aagcacaatt tttagcacca attatacctt gtcattattg
    18901 ctgcccctga tgatgtgggg agcaatgcgc tatggttata agctgatttc gctgctctgg
    18961 gcggtcgtgt tgatgatcag catccacagc tatcaaaatt acattcccat ttatcctggc
    19021 tataccacgc agctgaccat aacctcctcc agttatctgg tattctcttt tattgtcaat
    19081 tatatggctg tactggcaac tcgtcagcga gcggtagtca gacgcattca gcggcttgcg
    19141 tatgtggacc cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc gttgcgtgat
    19201 gccccctggt ctgcgctttg ttatttacgc atccctggca tggaaatgct gg
//
LOCUS       DAJTUN010000082        19214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTUN010000082 DAJTUN010000000
VERSION     DAJTUN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.082"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..154
                     /locus_tag="OO470_002616"
     CDS             <1..154
                     /locus_tag="OO470_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6251825.1"
                     /translation="LVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAV
                     GMTYQF"
     gene            complement(193..594)
                     /gene="crl"
                     /locus_tag="OO470_002617"
     CDS             complement(193..594)
                     /gene="crl"
                     /locus_tag="OO470_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6251826.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(652..1896)
                     /gene="frsA"
                     /locus_tag="OO470_002618"
     CDS             complement(652..1896)
                     /gene="frsA"
                     /locus_tag="OO470_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6251827.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(1988..2446)
                     /gene="gpt"
                     /locus_tag="OO470_002619"
     CDS             complement(1988..2446)
                     /gene="gpt"
                     /locus_tag="OO470_002619"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6251828.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2708..4165
                     /gene="pepD"
                     /locus_tag="OO470_002620"
     CDS             2708..4165
                     /gene="pepD"
                     /locus_tag="OO470_002620"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6251829.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4222..4836)
                     /locus_tag="OO470_002621"
                     /pseudo
     CDS             complement(4222..4836)
                     /locus_tag="OO470_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(4898..5941)
                     /locus_tag="OO470_002622"
     CDS             complement(4898..5941)
                     /locus_tag="OO470_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6251830.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(6187..6639)
                     /locus_tag="OO470_002623"
     CDS             complement(6187..6639)
                     /locus_tag="OO470_002623"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6251831.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(6649..7047)
                     /gene="yafO"
                     /locus_tag="OO470_002624"
     CDS             complement(6649..7047)
                     /gene="yafO"
                     /locus_tag="OO470_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6251832.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(7050..7343)
                     /gene="yafN"
                     /locus_tag="OO470_002625"
     CDS             complement(7050..7343)
                     /gene="yafN"
                     /locus_tag="OO470_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6251833.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYPNDMTDDDFNDFKE"
     gene            complement(7395..8450)
                     /gene="dinB"
                     /locus_tag="OO470_002626"
     CDS             complement(7395..8450)
                     /gene="dinB"
                     /locus_tag="OO470_002626"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6251834.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8521..9306)
                     /gene="lafU"
                     /locus_tag="OO470_002627"
     CDS             complement(8521..9306)
                     /gene="lafU"
                     /locus_tag="OO470_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6251835.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            9278..10990
                     /locus_tag="OO470_002628"
                     /pseudo
     CDS             9278..10990
                     /locus_tag="OO470_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(11207..11704)
                     /gene="rayT"
                     /locus_tag="OO470_002629"
     CDS             complement(11207..11704)
                     /gene="rayT"
                     /locus_tag="OO470_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6251836.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(11880..12401)
                     /locus_tag="OO470_002630"
                     /pseudo
     CDS             complement(11880..12401)
                     /locus_tag="OO470_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            12785..13525
                     /locus_tag="OO470_002631"
     CDS             12785..13525
                     /locus_tag="OO470_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6251837.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(13496..14263)
                     /locus_tag="OO470_002632"
     CDS             complement(13496..14263)
                     /locus_tag="OO470_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6251838.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(14469..15047)
                     /gene="lpcA"
                     /locus_tag="OO470_002633"
     CDS             complement(14469..15047)
                     /gene="lpcA"
                     /locus_tag="OO470_002633"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6251839.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            15287..17731
                     /gene="fadE"
                     /locus_tag="OO470_002634"
                     /pseudo
     CDS             15287..17731
                     /gene="fadE"
                     /locus_tag="OO470_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(17774..18220)
                     /gene="ivy"
                     /locus_tag="OO470_002635"
     CDS             complement(17774..18220)
                     /gene="ivy"
                     /locus_tag="OO470_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6251840.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            18401..19171
                     /gene="yafV"
                     /locus_tag="OO470_002636"
     CDS             18401..19171
                     /gene="yafV"
                     /locus_tag="OO470_002636"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6251841.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
BASE COUNT         4631 a         4994 c         4948 g         4641 t
ORIGIN      
        1 tctggtcaat tatatcgacg tcggtgctac gtattatttc aacaaaaata tgtcagcgtt
       61 tgttgattat aaaatcaacc aactggatag cgataataag ctgaatatta ataatgatga
      121 tactgtcgcg gttgggatga cgtatcagtt ttaatgaata ttgccggatg tgatgcatcc
      181 ggcacatttc actcacgccg ttaacttcac cggttcatca cgaaaatcat tcgccggttc
      241 cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact cacgcagggt
      301 gtgctccagt cgttcaacca cttcagtatc ttttaccgga acactcttcc agtcgcctgc
      361 tttatcgaac agaccaaact ggtagctgta ggtaaaacgg gattcctgcg cttccagctc
      421 catccaccag ccccagaatt cacgcacttc cggtgccggt ttcacgttga cgcatacagc
      481 cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag
      541 tgcggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg tcattgctat
      601 ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa
      661 gcgtttttcg atccagccgg tgatttcctg aagacctttg tcaaaattcc gatacaccgg
      721 gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta agcgtgagtc
      781 ctcttccggg ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg
      841 acgccccagc aatccttgca cttttaatga atagcgattc agctccacgc gtaacgcttc
      901 gtcggaagca tcatgcatcc ccaaacgact cgccagaacg tcaagataca tttccggcac
      961 ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac
     1021 cgctttcaga cgcggcgatt caaggtatgc cagacgcacg gcaacgttag cgccgaaacg
     1081 gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca gcgcctttaa
     1141 gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc cattttgaag aaaagcccac
     1201 cgacggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac gttcatacag
     1261 gctgtaatag tccgtctgca tcgcatccag accaccacac attaatactg tcgggaacgg
     1321 gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa
     1381 ctccatctgc cgcatcgtgc ccggcagacg ctgggcggcc tcttcatagg cgcggtttga
     1441 taaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt
     1501 gtacaacgta gccgcatgca gccagtgtcg accactgagt ttcggatctt cttccgtaca
     1561 ggctttttgt tgccacacca tcgcctgggt ggcccactca taaatccagt tgccaccacg
     1621 gtagccaatc acggtatcgt ataaatcatc gtcggtacgt tcagcatcgc tcatcacaat
     1681 acgtgcctgg acgtcgagga tttcacgtgg gtcaataccg cgccagatcc acatcagacg
     1741 gttaatcatg cgataccagt gagggatggt tttaccatca agagccgact gcacaggcgg
     1801 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
     1861 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
     1921 tggaaactat tgtaacctgc ctgaagttaa aaagaacaac gcccggcaat gccaggcgtt
     1981 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
     2041 cgatccaggt atcttgcggg atatcaacaa catagtcatc aaccagcgga cgaccagccg
     2101 gttttgcgaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaacagcag
     2161 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
     2221 gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
     2281 cgacatgacg aatacccagt tcacgcgcca gtaacgcccc cggtaccaga ccgccacggc
     2341 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
     2401 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
     2461 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
     2521 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
     2581 tccctgacgc gggataaagt ggtattctca atacatatct cgcaagcctg tcttgtgttg
     2641 acaacatttt ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat cgacaaggag
     2701 acttaacgtg tctgaactgt ctcaattatc tccacagccg ctgtgggata tttttgccaa
     2761 aatctgttct attcctcacc cgtcctatca tgaagagcaa ctcgctgaat acattgttgg
     2821 ttgggcaaaa gagaaaggtt tccatgtcga acgcgatcag gtaggtaata tcctgattcg
     2881 taaacctgct accgcaggta tggaaaatcg taaaccggtc gtcttacagg cccacctcga
     2941 tatggtgccg cagaaaaata acgacaccgt gcatgacttc acgaaagatc ctatccagcc
     3001 ttatattgat ggcgaatggg ttaaagcgcg cggcaccacg ctgggtgcgg ataacggcat
     3061 tggtatggcc tctgcactgg cggttctggc tgacgaaaac gtggttcacg gcccgctgga
     3121 agtgctgctg accatgaccg aagaagccgg tatggacggt gcgttcggct tacagagcaa
     3181 ctggttgcag gctgatattc tgattaacac cgactccgaa gaagaaggtg aaatctacat
     3241 gggttgtgcg gggggtatcg acttcacctc caacctgcat ttagatcgtg aagcggttcc
     3301 agctggtttt gaaaccttca agttaacctt aaaaggtctg aaaggcggtc actccggcgg
     3361 tgaaatccac gttggcctgg gtaatgccaa caaactgctg gtgcgcttcc tggcgggtca
     3421 tgcagaagaa ctggacctgc gccttatcga tttcaacggc ggcacactgc gtaacgccat
     3481 cccgcgtgaa gcctttgcga ccattgctgt cgcagctgat aaagtcgacg cactgaaatc
     3541 tctggtgaat acctatcagg agatcctgaa aaacgagctg gcagagaaag agaaaaatct
     3601 ggccttgttg ctggactctg tagcgaacga taaagctgcc ctgattgcga aatctcgcga
     3661 tacctttatt cgtctgctga acgccacccc gaacggtgtg atccgcaatt ccgacgtggc
     3721 aaaaggtgtg gtcgaaacct ccctgaacgt cggtgtggtg accatgactg acaataacgt
     3781 agaaattcac tgcctgatcc gttcactgat cgacagcggt aaagactacg tggtgagcat
     3841 gctggattcg ctgggtaaac tggctggcgc gaaaaccgaa gcgaaaggcg catatcctgg
     3901 ctggcagccg gacgctaatt ctccggtgat gcatctggta cgtgaaacct atcagcgcct
     3961 gttcaacaag acgccgaaca tccagattat ccacgcgggc ctggaatgtg gtctgttcaa
     4021 aaaaccgtat ccggaaatgg acatggtttc tatcgggcca actatcaccg gtccacactc
     4081 tccggatgag caagttcaca tcgaaagcgt aggtcattac tggacactgc tgactgaact
     4141 gctgaaagaa attccggcga agtaattatt tgatttgctg ccggatggcg tttaatcgcc
     4201 ttccggcagt ttcatccttc attatccttc gataaaagcc atccctgtaa acgttcgtcg
     4261 cgggttgcca cgttcaatct ggtgatggaa cattcgccgc tgtgatttca gcaccgcgct
     4321 attttcctat tgctgctgat ccagcttcca ggcaatcagc aatcgcgcca gccgtttgtt
     4381 agcatgctgg ctgcgctctg actgaacctt cacgctaata ccggatgcca aatgcgtggc
     4441 gcgtaccgcc gagtcggttt tattgacatg ttgaccgccc ggccccgacg aacgcagcgt
     4501 ctcataccgg atcgcatcgg attgctcctg ctcatcagcg gtaaaacgcc caatgcccag
     4561 aaaccagttt ttacgcccgt gatgtgggcg atacgggctc gggcaaatcc actgaatagt
     4621 gccacaccag ctttcgctta acgcccatgc gttgtcgcca tccagagaaa tcagcgccga
     4681 acgcagcgtg tcagagtagc ggcctgtttc tgtttccagc accgttaccg cgacgtcttg
     4741 tcgggcagct tctttaatta gcctgtccag cgcctttttc actgcgagac aacattcttc
     4801 cggcccctgc gctgaggaga gttatagcaa gatcatcctt ttttccctcc actgtttttg
     4861 agcgttagca ccggacgcag ccgcgcgaca ggaataatta atccggcctg cccagacatt
     4921 gcaccacact ttcagccgat ttataggctt gtggcgcttc ttcgaagatg agttgtttat
     4981 cgcgacaaat tacccgactg ccaagttcag tacgcgagag ttgcgtcgct gtgtatttcg
     5041 ctgccagacg ccctttacac tcggtgcgcc cccatttacg cccagcccca tgcgccagcg
     5101 aatgcaatgt ttcctcactc ctgacgggct gaaccagcct ggagtaatca ccgcgtgaac
     5161 cgggaataac caccagacca cagtcatcag gcgtggcacc tttacgatgt aagacccctt
     5221 gttgatcacc gattcgacac gcgctaacaa agttatgcgc cacatccaga accggacttc
     5281 cggtggcctt aacctgttgc attatgcgca aagcgatcag gtggcgatta aaacgcgcaa
     5341 acgccagcgc gtcgtcactg ccttcaggca aaccatgatg cgaaaacgag gcaatatgcc
     5401 gctgtaaaat agactgccct agccccctcg agccgctatg aaccagcagt tgcagatgct
     5461 gcgcatccag acctgccagt gcaaacagtt cagcgttgat gatttgatca acttgttgca
     5521 gttcagcgaa atgattgcca ccaccgatag agccaagcga gctgcgccac ggatgctgtg
     5581 caaatgctga cggcaggttt tcctccagcc agctttcttc agcaacgtca tccaaagctg
     5641 ataatcgctt ttcaaactta tcggcgttgt atttgcgagc gagaatatct gtttgccata
     5701 gcgccatgcc acagccgata tcattgccga ccagtgctgg gtaaaaacgg cctgcggaga
     5761 agaacgctgc gccaatcggg tagccgcgtc cggggtgtaa atctggcatc cccactacgc
     5821 gctgcatgtt gggtaaattt gccgtggtgt gtaattgttg gatcgctaaa ctctcgatcc
     5881 acaggtcatc agatgcgatg gtaaataccg catcggataa gggacgaata taattgccca
     5941 tataccattc cgtaaataga ctcgtcatac ttcaagttgc atgtgctgcg acttgaatta
     6001 ttgagagtaa caattaaaaa atcaggaaat ggcagacgaa tccagggcaa gaaaaaaaga
     6061 attaatcctg gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc
     6121 ttgcagtttg tggaataaga aaacgcgcac atactaggcg ggcgttattt tcattgcaag
     6181 ctggatttaa tgttgcggtt tataacgcat ataaaaatta ataaaccacg ctccccggca
     6241 ttcaacgcgc tgctgcttaa ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt
     6301 tatgcttgcg tccaccgtaa gttcggattc agacttaata aaaggtttta acaaagcgct
     6361 ggcaacccca cggcgggtgt attcagggtc aacaaataac atatcgatat agtgttcaac
     6421 gcaggtaata aagccgacca gttttgcatt aataactgca acccgcactt gtgatttcgc
     6481 gagtttctcc ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gttgtagtga
     6541 ataatgctga ctgacggtca tcgtaaccgc tctaatgaaa atagcgcata gttgctgaaa
     6601 atcgccagga tgataatctc ttatttgtat gttattcata atataaattc aaaaacgcat
     6661 gcgaaacgct tctgccattt ttccaatttt atgcatttgg ttgttatctc gagccagttt
     6721 atgaggttca ggcttcagaa tggcaatgag caaccatgct tgctcatcaa acgcaccctg
     6781 acaatatacc aaatgcgctt cgtcattcgt tctgctgaat tggcgcaact gtggcggaaa
     6841 tggattattc acatttgcca gatgaatatg agcaactcgc tcaaatttga ttaatggcca
     6901 ggtaaacgag tcgtcgtaga gtgcatcgcg accaaatata tctggcaaaa caccgtcacg
     6961 cttataggaa ataaaatccg ccgttaacgc atcaagttcc tctgctgtaa gttgcaggcg
     7021 aataagtttt gttttgaata cccgcatcct tattccttaa agtcattgaa atcatcatcc
     7081 gtcatatcat taggatattg tttagcgcgg cgtgtaattt cctctaatct tgcagcactt
     7141 ggacgaaagc gcgcctttat gggctttttc tcctcttgtt cagcaagcat gtccattaac
     7201 gcttcgaatg cgctggcact taagagatat cctgcggggc gattattaga aagaaccgca
     7261 actggttgat caataaagta tttagctggg tttttacgta actcagtgat attgaccgat
     7321 ttttcagcga gaattcgatg catacagtga taccctcata ataatgcaca ccagaatata
     7381 cataatagta tacatcataa tcccagcacc agttgtcttt ccatttgcgg gtcaagcaac
     7441 gtcacatgca gccccaccag acgcacaccg cgcccgccgc ggcgttcatc ccaggtttta
     7501 cgcgcggtgg cgattaagtc agctttattc agccgcggcc agacgtgctc ctgggtggtc
     7561 tgctgaaaat cgtcgaactt taatttcacc ccctggcgag caatcagtaa atcaggtttt
     7621 acctttgcca gacggcgttc aagttccgga tacagccgct cgataatcgc ttcacattca
     7681 gaccagtgat gaatatcttc cgccatcgtg cgttccacgc cgacggattt tcgtaaccgt
     7741 tcgctgttaa cgtcgcgctc gtcaatcccc tgactacgct cccacaaaat gcggccaaat
     7801 ttgccaaagc gtttaagcag aatcaccaga tcacactttt gtacatcacc gcaggtccgc
     7861 agccccatcg cttccagttt tgccgccgag actttgccga cgccggggat tttttccagt
     7921 ggtaaggttt gtaaaaatgc cggaacttct gccggcgtaa tcacaaactg accgttgggt
     7981 ttattcatgt cggaggcgat tttggcgaga aactttactg gtgccacgcc cgcagatgcc
     8041 gtcagttgca gctcgttgaa gatcgtctgg cggatttcct gggcgatgag ggtcgcagaa
     8101 ccgtggcaat ggacgctatc ggtgacatcg agataagcct catccagtga caacggttca
     8161 atgcgcgagg tgtagcgcga gaagatttca cggatatgat ttgaggcttc tttgtaggcg
     8221 tcaaagcgcc ccggaagcaa ggtgagatgt gggcataatt tgagcgccat cccagtaggc
     8281 atagcgctac gtacgccaaa tttacgcgcg ggataattgg cggtgctgat caccccccga
     8341 cgttcgcggc tgccgccaat agcaataggg atatcgcgca gagcgggatt gtcgcgcatc
     8401 tccaccgctg cgaaaaagca gtccatatcc acatgaatga ttttacgcat tgctcacctc
     8461 tcaacactgg taaagtatac agtgatttca ggttttgaga aatgcgtaaa gattcagcat
     8521 ttagcgcatt cgctgcgaaa gcacctgctg cttatccagc ttttgcacca gcggctgcac
     8581 cactttatcc ccatgctgac caaagtattg atacagcgta tcggacgcac ttttggtcag
     8641 caccataatc tcaatacgcc gattgcccgc gctttgcgga ttcttcgcgt ccagcagcat
     8701 ctggtccgcc attgcgctta cctgcatcac tttatcttcc ggcattccgg cctcttccag
     8761 cacccgacga gctgaaagcg cgcgatcgcc cgaaaggttc cagttgttgt agatattgtt
     8821 tttgtaggcc atcgcatcgg tatgcccggt aataataatt ttgttataga gcgagtcgaa
     8881 cactggcgcg agctccacca gcaaagtttt aaagaacggc ataatctgcg cgctgccgcg
     8941 ttcgaacata ttgcgattct ggtcgtcttt aatcagcacg cgtaatccct ggggaacaat
     9001 ctccatctcc agattcgctt ccatatgcgc atcacgggca atggtgttga tactggttgc
     9061 caactcgcca agctcggtgg cggatttctt cttcagcagc gccgttttct cattcacatc
     9121 acgcgctttt ttctccgtct cttccggcac cgccacggtg gcaggttttg gcggatgaga
     9181 cgggctgatt ttatttaacg gcgacagccc accaccgtta aaaatcgact gcccgtgcag
     9241 cgcggcgata atgctttcac gctcggattt actgacgctg ttgacaatca actttatcgt
     9301 cgtcaccaaa gaggccgagc gtatttccga ggtttctgcc cgctttaccc tggacgcgat
     9361 gcccggcaaa cagatggcga ttgacgccga tcttaacgcc ggattgatca accaggcgca
     9421 ggcgcaaacc cggcgtaaag atgttgccag cgaggccgat ttctacggcg cgatggacgg
     9481 ggcatcgaag tttgtacgcg gagacgccat cgccgggatg atgattctgg cgatcaacct
     9541 gagcggcggc gtctgtatcg gcatcttcaa atacaacctg agcgccgacg ccgccttcca
     9601 gcaatacgtg ctgatgacca tcggcgacgg cctggtggcg cagatccctt ccctgctgct
     9661 ctccaccgcg gcggcgatta tcgtcacccg cgtcagcgac aacggcgata tcgcccatga
     9721 cgtgcgccac cagttgctgg caagcccgtc ggtgctctac accgccaccg ggatcatgtt
     9781 cgtgctggcg gtggtgccgg ggatgccgca tctgccgttt ttgctgttca gcgccctgct
     9841 tggctttacc ggctggcgga tgagcaaacg cccgcaggcg gcggaggcgg aagagaaaag
     9901 cctcgaaacg ctgacccgca ccatcactga aaccagcgaa caacaggtca gttgggaaac
     9961 tattccgctg atcgagccca tcagcttaag cctcggctac aagctggtgg cgctggtgga
    10021 caaagcgcag ggcaacccgc tcacccagcg gattcgcggc gttcggcagg tgatttccga
    10081 cggtaacggc gtgctgctgc cggagatccg cattcgggaa aacttccgcc tcaagcccag
    10141 ccagtacgct attttcatca acggcattga ggctgatgaa gcggatattc cggcggataa
    10201 actgatggcg ctgccctcca gcgaaaccta cggcgagatt gacggcgtgc tggggaacga
    10261 cccggcgtac gggataccgg tcacctggat ccagcctgcg cagaaagcga aggctctgaa
    10321 tatggggtat caggtgatcg acagcgccag tgtgattgcc acccatgtga acaagattgt
    10381 gcgcagctat attcctgatt tgtttaacta tgatgacatc acgcagttgc ataaccgttt
    10441 gtcgtcgatg gcaccgcgcc tggcggagga tttaagcgcg gcgctcaatt acagccaatt
    10501 gctgaaagtg taccgggcgc tgctgaccga aggcgtttcc ctgcgcgata tcgtcaccat
    10561 cgccaccgtg ctggtcgcca gtagcacggt gactaaagat catattctgc tggcggccga
    10621 tgtgcgcctg gcgctgcggc gcagcattac tcatccgttc gttcgcaagc aggagctgac
    10681 ggtgtatacg ctgaataatg agctggaaaa tctgctgacc aacgtagtga atcaggcgca
    10741 acaggccggc aaagtgatgc tcgacagcgt gccggttgac ccgaatatgc tcaaccagtt
    10801 ccagagcacg atgccacagg tgaaagagca gatgaaagcg gcggggaaag atccggtgct
    10861 gctggtgccg ccgcagctac gccctttgct ggcgcgttat gcaaggttgt ttgcgccggg
    10921 gctgcatgtg ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga ttatgggggc
    10981 gttgatgtaa gaatccggca tcggtgcaca attgccggat gcggcgtaaa cgccttatcc
    11041 ggcctaccgt tcaggcacaa gtttttaggc atgataagac gcgccagcgt cgcatcaggc
    11101 atctgcgcat aactgccgga tgcggcgtga acgccttatc cggcctaccg ttaaagcacg
    11161 atttgtaggc cggataagcg taagcgcatc cggcaccagc gcacagttat aaaatgatac
    11221 gctccccggc acttaaatcc gttatgtctc ccgcccaatc gatgggatat aaccctcgcg
    11281 cgacatcgcg atggaacgtt gagaatggcc aatcactcac ctgctttacc cagccatgtt
    11341 tctctggatt tatataaata taatcaacat gatggcgata atcttttgta ttgcgaatgg
    11401 catgctccca aaaacgcggt tgccagatat ttttcaatcc gcaagcatgg gtgaactgct
    11461 ttttaatctc ccgccagcga gaggaaaaat catcatcgct ttcgggtaat gtccatatgc
    11521 aatgcatatg ctccggcaaa acgacccagg cgttaatttc aaaaggtctg tctcgcttaa
    11581 cgttaataat ggcgttacgg agcgtctgga actgggtggt cagaagatgg cttcgacgat
    11641 tgcgtaaatt taccgtgaaa aaccatgttc cccccttgat gtaataacga cgatattccg
    11701 acatagcgac tcctttttct caacggtaag aggaaaagga aaggccgaca atgacgctgt
    11761 gttatatcgg gaaagcggct cgcaaaaacg cacagatttg taggcatgat aagacgcgtc
    11821 agcgtcgcat caggcatccg tgcacaaccg ccggatgcgg cgtaaacgcc ttatccggcc
    11881 tacaaaatca tctcttccgt caaaatcctg cgcgcgccta aaaaatggtc ctgccagaaa
    11941 ggttcggcta accggcttac ccgaatggtt tccccggtgc gcagtgactc aataaactga
    12001 ccgtcgccca gatacacccc catatggtcg gcaatctcgc ggctgtggat atggaaaaat
    12061 agcaaatctc cccggcgcag gtcgttattc gccacaatcg ttgcccgacg gtagtggtac
    12121 atctcattgg ccgtgcgcgg gagtttcgcc tcaaggatct tgttgtaggc ataaaaaact
    12181 aacccactac aatcgaagcc ttgatcgggc cgcgtgccgc cccagacgta aggcttgcca
    12241 agctgctgct ccaggcggtg aatcgccact tcggtgatgt tatgcaggtg atcgctgctt
    12301 aaaaagtggt tgttttccgc cagcgcctgc cagcgtctgt cactcgcctt gagcggcgca
    12361 ggaaaccatt ctggatgctg ttttatctgc tcccggttgt ggtggcgctg gcgagtaaag
    12421 ggagcaaaac gcataacgaa aacacgcctg gatgcaaaaa tctgccgagc aagaacgaag
    12481 acatgaaaga cataaaccgg ctgaatgaag gtgagaattt taaaagaccg gaggagtaaa
    12541 cgggattatt aattgcttat ttgcatatta ctattgagat ttcataatat taggaaataa
    12601 atgatacatg cgcttttcgg actattgaaa actgcccctg tgctaaattt gtaaacccag
    12661 cgccgacagg cttgcgtaaa actcttttgg tgataatgtg agccttccaa atccagacta
    12721 ttccgatttg ggatccattt tgccgtcctg gcgtgtgtaa ccgttttatc aaggaataag
    12781 cagtatgcgt aaaatcgcat taattcttgc gatgttgttg atcccgtgcg tttcgtttgc
    12841 cggtttgctg ggcagcagta gctcgaccac gccagtgagc aaagagtata agcagcagtt
    12901 gatgggatcc cctgtctaca tccagatctt caaggaagaa cgtacgctcg atctttacgt
    12961 caaaatgggc gagcaatatc aactgctcga cagttataaa atctgtaaat attccggcgg
    13021 cttagggcca aaacagcgtc agggcgattt caaaagcccg gaagggtttt atagcgtcca
    13081 gcgtaatcag ttaaaaccag acagccgtta ctacaaagcg attaatattg gattccccaa
    13141 tgcttatgac cgtgcgcatg gttatgaagg gaaatacctg atgatccacg gcgattgtgt
    13201 atccatcggc tgctacgcaa tgaccaatca gggtattgat gagatcttcc agttcgttac
    13261 tggtgcgctg gtgtttggtc agccgagcgt gcaggtgagt atttacccat tccgcatgac
    13321 cgacgccaat atgaagcgcc ataaatattc caactttaag gacttctggg agcagctgaa
    13381 gccgggctac gactactttg agcaaacccg taagccaccg accgtttctg tggccaatgg
    13441 ccgttacgtg gtcagcaagc cgttaagcca cgaagtggtg cagccacaac tggcatcaaa
    13501 ctacacgctc cccgaggcaa aataagtgcc attcgcctgg cataattttt tgccaggttt
    13561 cattgcccgt cagcggctgt gtcgcaatca ccgtgaccac atcattcggt gtggtctgcg
    13621 agctgaagtc gatttccaca tcctgatcca gcaacgttgc cacgccaaac ggcgcgcggc
    13681 gggtgatcca gtgtaaatta gtcgagcaat acgccattac atagcgcccg tccgaaagca
    13741 gcatgttgaa aacgcccttc gcccgcagtt catccgccag tgaggcgata tatttaaaca
    13801 ctgccgccat gttgcccggc gtgcgcgggt aacgctgcgt taatttatgc agcagccagc
    13861 aaaaggcttt ttcgctgtcg gtttcaccga ccgggcggaa gttgccggtt tccagtgatt
    13921 tgtagcccgt cagttgtccg ttatgggcat aagtccagtt acgcccccat aactcgcggg
    13981 taaatgggtg agtattttcc agcgccacct cgccccgatt agcctggcga atatgagcca
    14041 ccaccgaaca ggattttatc gggtagtcct ggacaagttt ggcgatgggg gaattaaagc
    14101 tgggttgtgg atctttaaat gtgcgacagc ctttaccttc gtaaaaggta atgccccagc
    14161 catctttatg tggcccggtt ccaccaccac gctgtacaag tccggtgaaa ctaaagcaga
    14221 tatcggtagg gacgttggcg ctcatcccga gcaattcgca catgggtaac ctccacaaca
    14281 gcgggagcgc atggaccccg gcgaagattg atttaagaat tttcaggtcg gatgcggcgt
    14341 aaacgaatta ttcggcctac ggttcgggta cagatttgta ggcatgataa gacgcgtcag
    14401 catcgcatca ggcatcagcg cacaagtgcc ggatgcggcg taaacgcctt atccggccta
    14461 cgccagactt acttaaccat ctctttttca attaactgga tcaggatatg gatcacttta
    14521 atgtgaatct cctgaatgcg gtcggcataa ccaaagtgcg gaacgcgaat ttcgatatcc
    14581 gccgtgccag ccattttacc gccgtcttta ccggtcaggg tgatcacttt catccccttc
    14641 tcacgcgccg ctgcgatcgc tttgatcacg tttgcagagt taccggaggt ggagatcccc
    14701 agcagtacat cgccttcgcg acctaccgct tcaacgtagc gggagaaaat atcattgaaa
    14761 ccgaaatcat taccgacgca ggaaatatga ctaacgtcag aaatagcaat cgccgggtag
    14821 cccggacggt tttcacggta gcgaccggtc aactcttcgg caaagtgcat agcgtcgcaa
    14881 tgggaaccgc cgttgccgca ggaaagcact ttgccaccgg ctttaaagct gtctgctaac
    14941 aggaccgccg cgcgctgaat ggcgtgaata ttggcgtcat cttttaaaaa gttagccagc
    15001 gtttccgccg cttcgttcag ttcgttacga ataagatcct ggtacatgag gatatccttc
    15061 agcataaatg taatagacaa aatgcagtgt accggatacc gccaaaagcg agaagtacgg
    15121 gcaggtgcta tgaccaggac tttttgacct gaagtgcgga taaaaacagc aacaatgtga
    15181 gctttgttgt aattatattg taaacacatt gctaaatgtt tttacatcca ctacaaccat
    15241 atcatcacaa gtggtcagac ctcctacaag taaggggctt ttcgttatga tgattttgag
    15301 tattctcgct acggttgtcc tgctcggcgc gttgttctat caccgcgtga gcttatttat
    15361 cagcagtctg attttgctcg cctagacagc cgccctcggc gttgctggtc tgtggtcggc
    15421 gtgggtactg gtgcctctga ccattatcct cgtgccattt aactttgcgc ctatgcgtaa
    15481 gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag gtgatgccgc cgatgtcgcg
    15541 cactgagaaa gaagcgattg atgcgggcac cacctggtgg gagggcgact tgttccaggg
    15601 caagccggac tggaaaaagc tgcataacta tccgcagccg cgcctgaccg ctgaagagca
    15661 agcgtttctc gacggcccgg tagaagaagc ctgccggatg gcgaatgatt tccagattac
    15721 tcatgagctg gcggatctgc cgccggagtt gtgggcgtac cttaaagagc atcgcttctt
    15781 cgcgatgatt atcaaaaaag agtacggcgg actggagttc tcagcttatg cccagtctcg
    15841 tgtgctgcaa aaactctctg gcgtgagcgg gatcctggcg attaccgtcg gcgtgccaaa
    15901 ctcattaggc ccgggcgaac tgttgcaaca ttacggcact gacgagcaga aagatcacta
    15961 tctgccgcgt ctggcgcgtg gtcaggagat cccctgcttt gcactgacca gcccggaagc
    16021 gggttccgat gcgggcgcga ttccggacac cgggattgtc tgcatgggcg aatggcaggg
    16081 ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc tacattacgc tggcaccgat
    16141 tgcgaccgtg cttgggctgg cgtttaaact ctccgacccg gaaaaattac tcggcggtgc
    16201 agaagattta ggcattacct gtgcgctgat cccaaccacc acgccgggcg tggaaattgg
    16261 tcgtcgccac ttcccgctga acgtaccgtt ccagaacgga ccgacgcgcg gtaaagatgt
    16321 cttcgtgccg atcgattaca tcatcggcgg gccgaaaatg gccgggcaag gctggcggat
    16381 gctggtggag tgcctctcgg taggccgcgg catcaccctg ccttccaact caaccggcgg
    16441 cgtgaaatcg gtagcgctgg caacgggcgc gtattctcac attcgccgtc agttcaaaat
    16501 ctctatcggt aagatggaag ggattgaaga gccgctggcg cgtattgccg ggaacgctta
    16561 tgtaatggat gctgcggcat cgctgattac ctacggcatt atgctcggcg aaaaacctgc
    16621 cgtgctgtcg gctatcgtta agtatcactg tacccaccgc gggcagcagt cgattattga
    16681 tgcgatggat attaccggcg gtaaaggcat tatgctcggg caaagcaact tcctggcgcg
    16741 tgcttaccag ggcgcaccga ttgccatcac cgttgaaggg gctaacattc tgacccgcag
    16801 catgatgatc ttcggacaag gagcgattcg ttgccatccg tacgtgctgg aagagatgga
    16861 agcggcgaag aacaatgacg tcaacgcgtt cgataaactg ttgttcaaac atatcggtca
    16921 cgtcggtagc aacaaagttc gcagcttctg gctgggcctg acgcgcggtt taaccagcag
    16981 cacgccaacc ggcgatgcca ctaaacgcta ctatcagcac ctgaaccgcc tgagcgccaa
    17041 cctcgccctg ctttctgatg tctcgatggc actgctgggc ggcagcctga aacgtcgtga
    17101 gcgtatctcg gcacgtctgg gggatatttt aagccagctt tacctcgcct ctgccgttct
    17161 gaagcgttat gacgacgaag gccgtaatga agccgatctg ccgctggtgc actggggcgt
    17221 acaggatgcg ctgtatcagg cagaacaggc aatggacaat ttactgcaaa acttcccgaa
    17281 ccgcgtggtt gccgggctgc tgaatgtggt gatcttcccg accggacgtc attatctggc
    17341 accgtctgac aagctggatc ataaagtggc gaagatttta caagtgccga acgccactcg
    17401 ttcacgtatt ggtcgcggtc agtacctgac gccaagcgag cataacccgg ttggtttact
    17461 ggaagaggcg ttggtggatg tgattgccgc cgacccgatt catcagcgga tctgtaaaga
    17521 gctgggtaaa aatctgccat ttacccgtct ggatgaactg gcgcacaacg cgctgacgaa
    17581 ggggctgatt gataaagatg aagccgctat tctggtgaaa gcggaagaaa gccgtctgcg
    17641 cagtattaac gttgatgact ttgatccgga agagctggcg acgaagccgg taaagttgcc
    17701 ggagaaagtg cggaaagttg aagccgcgta attgaataac ggagccgaaa ggctccgttt
    17761 ctttatccgc taattattta aaattaaagc catccggatg gttttccagg ctgccggtca
    17821 acgccgcgaa cagcaccgtt ttaccatcaa tcgaaagcgc atcgttcaca ttcagccagg
    17881 tgagtttctc ttgcgacgtt ttctcatcaa tagccgagaa cagccccgtc atctgattag
    17941 atttctcgga ccacatcaca gcgatacgtt gcgagccaca gtcatgcggt ttgcacgcgc
    18001 tcatcacctg atacgtctca tctcccaacg ttacggtttg tgcgggagta taagtaccgc
    18061 ctttcatcac ccatgcaggt agcttatgcc cttgcaccat ctgattaaac gcagctttgg
    18121 tggtttcgcc ctttgcaagg ctgctaatgg ttaaatcatc ctgcgccatt gcactggtgg
    18181 cgatgaccag tgcggcgact gtcgttattg ccttaaacat cattcctccc gagcttatcc
    18241 tgcccatgtt attaacctcc taaagcctgg cgataacacg ataatttgca aatcagatat
    18301 ttccaatatc agcatttgtt atcaatttat tctgcgctaa ggcaactgcc tgataaggtg
    18361 ttaaagttat aaaacccttc gcataaagga gttgtacacc gtgcctagtt tgaagattac
    18421 gcttttgcag caaccgttgg tgtgggtgga tggtcctgcc aacctgcgcc attttgatcg
    18481 tcaactggaa ggtattaccg ggcgcgatgt gatcgttcta ccggagatgt ttaccagcgg
    18541 ctttgccatg gaagcggcag cttcgtcgct tgcacaagat gacgtagtga actggatgac
    18601 agctaaggcg cagcagtgca atgcgctgat tgcaggcagt gttgcattac aaacggagtc
    18661 tggttcggtt aaccgctttt tgctggttga gccgggcggc acggtacatt tttatgataa
    18721 gcgtcatctg ttccgcatgg cagatgagca tctgcattat aaagcgggca atgcgcgagt
    18781 gattgtggaa tggcgcggct ggcgtatttt gccgctggtg tgctacgact tacgttttcc
    18841 agtgtggtcg cgcaatctca acgattatga cctcgccctg tacgtcgcca actggccagc
    18901 tccgcgctct ctgcactggc aggcattgct gacggcccgc gcgattgaga atcaggcgta
    18961 tgtggcggga tgcaatcgcg tcggcagcga tggcaacggc tgccattatc gcggtgacag
    19021 ccgggtgatt aatccgcaag gcgagattat cgctaccgcc gacgcgcata aggcaacgcg
    19081 tattgatgcg gagctgtcga tggtggcatt gcgggaatat agagaaaagt ttccggcatg
    19141 gcgggatgcg gatgagttta ggttgtggtg agtgggggtt gtgtttaggg ccgggggctg
    19201 ggctagatta cgaa
//
LOCUS       DAJTUN010000083        18248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTUN010000083 DAJTUN010000000
VERSION     DAJTUN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.083"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            35..1642
                     /locus_tag="OO470_002637"
     CDS             35..1642
                     /locus_tag="OO470_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6251842.1"
                     /translation="MPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIG
                     QKLAAADIQDANFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSE
                     RAQLLAERFDGVCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVIN
                     NAVEALKVIAEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPG
                     ENIRDNALNFHKWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKST
                     FTHIASLLAGKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGG
                     DPAEINPKYEKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDR
                     ELPEKIAAEIPVIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLN
                     FLEEARGLMMGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVY
                     GHEYITRKVKGVTQTNAITTDECDAFL"
     gene            1931..2209
                     /locus_tag="OO470_002638"
     CDS             1931..2209
                     /locus_tag="OO470_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251843.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2206..2511
                     /locus_tag="OO470_002639"
     CDS             2206..2511
                     /locus_tag="OO470_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251844.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2521..2682
                     /locus_tag="OO470_002640"
     CDS             2521..2682
                     /locus_tag="OO470_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6251845.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            2824..3048
                     /locus_tag="OO470_002641"
     CDS             2824..3048
                     /locus_tag="OO470_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251846.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3349..4506
                     /locus_tag="OO470_002642"
                     /pseudo
     CDS             3349..4506
                     /locus_tag="OO470_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4546..5118
                     /locus_tag="OO470_002643"
     CDS             4546..5118
                     /locus_tag="OO470_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6251847.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5120..6331
                     /locus_tag="OO470_002644"
     CDS             5120..6331
                     /locus_tag="OO470_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6251848.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6328..6666
                     /locus_tag="OO470_002645"
     CDS             6328..6666
                     /locus_tag="OO470_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6251849.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            6663..6959
                     /locus_tag="OO470_002646"
     CDS             6663..6959
                     /locus_tag="OO470_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6251850.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            6959..7399
                     /locus_tag="OO470_002647"
     CDS             6959..7399
                     /locus_tag="OO470_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6251851.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7383..7565
                     /locus_tag="OO470_002648"
     CDS             7383..7565
                     /locus_tag="OO470_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251852.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            7621..7911
                     /locus_tag="OO470_002649"
     CDS             7621..7911
                     /locus_tag="OO470_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6251853.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            7931..9592
                     /locus_tag="OO470_002650"
     CDS             7931..9592
                     /locus_tag="OO470_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6251854.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            9606..9887
                     /locus_tag="OO470_002651"
     CDS             9606..9887
                     /locus_tag="OO470_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251855.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(10684..12144)
                     /gene="phoQ"
                     /locus_tag="OO470_002652"
     CDS             complement(10684..12144)
                     /gene="phoQ"
                     /locus_tag="OO470_002652"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6251856.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12144..12815)
                     /gene="phoP"
                     /locus_tag="OO470_002653"
     CDS             complement(12144..12815)
                     /gene="phoP"
                     /locus_tag="OO470_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6251857.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(12984..14354)
                     /gene="purB"
                     /locus_tag="OO470_002654"
     CDS             complement(12984..14354)
                     /gene="purB"
                     /locus_tag="OO470_002654"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6251858.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14358..14999)
                     /gene="hflD"
                     /locus_tag="OO470_002655"
     CDS             complement(14358..14999)
                     /gene="hflD"
                     /locus_tag="OO470_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6251859.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15035..16141)
                     /gene="mnmA"
                     /locus_tag="OO470_002656"
     CDS             complement(15035..16141)
                     /gene="mnmA"
                     /locus_tag="OO470_002656"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6251860.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16195..16656)
                     /gene="nudJ"
                     /locus_tag="OO470_002657"
     CDS             complement(16195..16656)
                     /gene="nudJ"
                     /locus_tag="OO470_002657"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6251861.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(16666..17319)
                     /gene="rluE"
                     /locus_tag="OO470_002658"
     CDS             complement(16666..17319)
                     /gene="rluE"
                     /locus_tag="OO470_002658"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6251862.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17491..>18248
                     /gene="icd"
                     /locus_tag="OO470_002659"
     CDS             17491..>18248
                     /gene="icd"
                     /locus_tag="OO470_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6251863.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREE"
BASE COUNT         4712 a         4499 c         4905 g         4132 t
ORIGIN      
        1 gcaggagcag gatggccccg catccggcga taatgtgccg cctgtgtggc ttgggccaaa
       61 tcagcttgcc gaacttgatg cactgaaaat tgttccggat ggaaaaaaac gcgtaaggct
      121 gtaccaggcc ggagaactgg atttggtgga gaccaaaaag attggtcaga agctggcggc
      181 ggcagatatt caggacgcaa atttttaccc cgaaggaatg cacgtccaga agtgtgaaaa
      241 ctggcggcgc tatctgaatg ctgagcgtga aaatattgcc gcagggctta ccatgccgga
      301 gcagaaaaat acgcaactgg cacaaatggc agacagtgag cgcgcgcagc tgcttgccga
      361 gcgctttgat ggcgtttgtg tgcatcagga aagtgaaatc gttcacgtat ggcgcggcgg
      421 ggtatggtgt ccggtcagca caatggaact tagccgcgaa atggtggcga tctattcaga
      481 gcacagggcc actttcagca agcgcgtaat caataacgcc gtggaagcgt taaaagttat
      541 tgccgaaccc atgggcgagc cgtccggcga tttgctgccg ttcgccaatg gtgcgcttga
      601 cctgaaaacg ggggaatttt ccccgcacac gccggagaac tggatcacca cgcacaacgg
      661 cattgagtac acgccaccag caccagggga gaacatccgc gataacgcgc taaactttca
      721 taaatggctt gagcacgcag ccggaaaaga ccagcgcaag atgatgcgta tatgtgccgc
      781 gctgtacatg attatggcga accggtacga ctggcagatg tttattgagg ccaccggaga
      841 cggcgggagc ggtaaaagta cattcacaca catagccagc cttctggcag ggaaacagaa
      901 cacggtaagc gctgaaatga catcgcttga tgatgctggt ggacgtgcgc aggttgtcgg
      961 gagtcgtctt atcgtcctgg ctgaccagcc gaaatataca ggcgaaggaa cgggcatcaa
     1021 gaaaatcacg ggcggtgacc ccgcggaaat taacccgaaa tacgaaaagc gtttcacggc
     1081 tgtaatcagg gcggtggtgc tggcgaccaa caataacccg atgatattca ccgaacgggc
     1141 cggaggtgtg gcacgtcgtc gcgtgatttt ccgtttcgac aatatcgtta gtgaggccga
     1201 aaaagacagg gagctaccgg aaaagattgc ggctgaaatc cctgtcatta tccgccgatt
     1261 gctggcgaac tttaccgact ctgagaaagc acgggcttta ctactggaac agcgtgacgg
     1321 tgatgaagct ctggcaataa agcaacagac ggatccggtt attgagtttt gccagttcct
     1381 gaattttctg gaggaagcac gcggcctgat gatgggcggc ggtggcgatt cagtgaagta
     1441 cacgaccaga aacagccttt accgcgtcta tctggcgttt atggcgtacg caggcaggag
     1501 caaaccgcta aacgtaaatg actttggcaa ggctatgaag ccagccgcga aagtttacgg
     1561 acatgaatat attacgcgga aagttaaggg agtaacgcag actaacgcaa taacaacaga
     1621 cgagtgcgac gcgtttttat aattttttgt aattgctgtc taccctgtct acctgagtaa
     1681 agaaaaatac atttaattca gtgcattaag ttaggtagat agcctttttt tactgtctac
     1741 ccactatcta ccctctctac ctgattttac ctgaatcaga cagggaggta gacacggggt
     1801 agatagtgga taaaagcact ctaccccgct gaaagcagcg ccattactgg catgagggcc
     1861 actaaggtag ataaggtaga caaggggagg cacaactcaa aactttttaa acgagggggt
     1921 aaaaataaat atgcacactt cagggaaatt taataaatca ttcaaaaaac gcagagacag
     1981 aacagaaccg aaatatcgcg cgttagacat gacagagcac gctttaaagg tggcaatcag
     2041 aacgatagac cgccacgcgg gggaaggata cgcgaaagca catcccgaac tgataagcgc
     2101 attcatgacc acgacggcgg caaattttgc cacgctgaca gagcgggaga ttgccgaagc
     2161 ggaacaggta acaacaatca acgttaaaac cggagaggtg gaatcatgag cgaaccaaaa
     2221 tttggtgaaa aattttacaa gcacaacgga cggatcacga ttctgcaaat aagcgcagct
     2281 acaccaggct ggtgggttga aacagacgaa ggatcctcac ccgtggcatc atgggcgctt
     2341 tgtgtgctta gttaccctga ccgcgatgtg tatcaggata ttctgcctgt gatatccact
     2401 gacaaaggca tgaaacccgt agacatcaaa aacatggggt ttcagtgcgt aatgctcact
     2461 gaaaaaatga tggaagagat gaaaaagaac aatgccggat cgcttcactg aggtggcaat
     2521 atgacagcac aaattgcagc ttacgggcgg ctggtgggcg acccgctggt aaaacagagc
     2581 agcaagggca caccggagcc gccaacggag aactacgcag acctgagaaa tgcagtaaat
     2641 cgcacttacg cagacatggg gctgagcaaa ttagcagagt aggaatatca gaaccacacc
     2701 acgcagccgg agcaatccgg ctttttttgc gtcatttgaa acggtatgga gcactactgg
     2761 cttttcgtca cggtccggca tagttactat ctgaaacaaa cagacacaac agaggaaaac
     2821 gcaatgccga tgaaatttga tgagatactt aaacagcgtg ataaatacca tgctgacaac
     2881 atggagacga tgaatatcac tgattaccgc gcatttctgg aaacgggggc gctgattgaa
     2941 aaggatcatc atggttttgt tcgttgcgcg ctatccggtg aaatgctggc agtaaatcct
     3001 gaacagacag atgcattgat agaatttctg aaagggatca gagactgagc acccatacag
     3061 cacatacagc cggagcaatc cggctttttg ttattttttg taaattattt gttcgtggtt
     3121 gttccacgtt gttcactgag cggatcggca tattttaccc gaactgaatc atgattattc
     3181 tcgcccgtgg tgccaggacg ctggggccac tttcccgcct gttaatgtgc tcgccaatat
     3241 tcattaccag gcgggaaaac gatcggtgcg attactgatt tccttatgaa aaacggttga
     3301 gtgtttgccg cgtcctggag ttccttactt aaccccagga ctttttttat gcagagaata
     3361 atcgaattac gccagcagaa aaccgccatt aaaaatcaga tgcgcgacat gctggagaac
     3421 gctgaaaaag aaaaccgcag tcttaacgat gctgagggca caaaatttga cgaattacgc
     3481 gctaaatctg aatccctcga taaagacatt tcccgccttg aagccattgc agacgaagag
     3541 cgcagcaagc caggtaaaag cagccagacc actgaccccg cagaactacg caactacatt
     3601 ctgaccggtg aaacccgcgc attaagtaca ggcgttcccg ctgatggtgg ttataccgtt
     3661 atccctgaac tgaacaccga aatcatgcga atgctggcgg atgaatccac catgcgtcgc
     3721 atctgtaccg tgaagaaaat cagcagcaac gagtttaagc agcttgtttc cgctggcggt
     3781 gcgaccgtta accacggtga agagggtaag gcacgcgaac agaccagcac cccgcagatt
     3841 aacgaggtga gcattaagct gtatccggtc tatgcgtacc cgcgcaccac acaggaaatc
     3901 gtggattttt ccgatgtgga catcctttca tggctgacgg gtgagattgg cgacaccttc
     3961 acggaaaccg aagaaagcga tctggttgtg ggcgacggtg acaaaaaagc aaaaggtttt
     4021 ttatccgtac cccgtgcaga gaagaacgac aaagagcgtg attttggtac gttgcaggta
     4081 attaaacctt ccgaatctct ggcgtggaca tctgcggacc cgctgatcga cctgaaattt
     4141 gcattacgta aaaaataccg caaaaacgcg gtctgagtgg ttaactccac gacggcggca
     4201 aaacttcaga aggtgaagaa cgcgaacggt gattatatct ggcgcgaccg tttacagacg
     4261 ggtgatcctg atacgttgct gggccttccg gtcgaatatc tggagtttat gcctgataac
     4321 gttattgccc tgggtgactt caaacgcggt tactacattg ttgatcacga aacaggtgtt
     4381 cgcaccagac cggacaacct gacagaacca ggattcatca aaattttcac gcaaaaatat
     4441 ttaggcggtg gcgtggtgga ttcgaacgcg atcaagattc tggcactgcc ggaagacgac
     4501 gattaacagc atacagaagg ggctaaaaag cccctttagt gttttatggg tgaaaaaatt
     4561 atgaagagta tggaaatccg gtcatcggaa atcaccacca gcggagccgg tacgctgacg
     4621 ggctacgttg ttcgctggga taagctttca gaactgttat ggggtgagtt ttacgaaaaa
     4681 ttccagcggg gggcgtttac tgaatggctt gcggcgggta atgacgttcg cggcctgtat
     4741 gagcatgacc acagcatgtt actggggcgc acccgttccg gcacgctgaa actggaagag
     4801 gacgaaacag ggttacgctt tgaactgacc ccaccggata ccagtacagg gcgcgacgtt
     4861 atcgaactgg ttaagcgcgg ggacatctcg gggatgagct ttggctttcg ctcccgtaag
     4921 gatgtatggg ataccacaac agatccatgc gtgcgcaccg tgctggtggc ggaactgtac
     4981 gaaattaccg ttacatcggt accggcttac cccgattccg gcgtggagct ggcccgccgt
     5041 tccctgtatg agcagcaccc cgaaaaaatg ccgcgtgcgg ataatcgccg ctggtgggcg
     5101 gatttagcgg gggtgtgata tgtggccttt cagacgaaaa aaagagcagc gcagcatgac
     5161 gcttgatgaa tttatggcgc tggccggcac atcgaacacg ggggcgggtg agtacgtatc
     5221 atctgggaca gcggaatcac tccccgccgt catgaacgcc gtcacggtca tctctgacgc
     5281 ggtggctacc atgccgtgtt acctgtacct ggtacgcaat gagaagggga aggaggcccg
     5341 cgaatggctt gattctcatc cggtcgatca catcctcaac gagcgcccga acgcgtggca
     5401 gacaccctac cagtttaagc gaatgatgat ccgctactgc ctgttaagtg gtaatgctta
     5461 tgcggttatt cagtgggggc gtgatggttt tccggcggct ttacatcctt acccgccgca
     5521 gtcggtgaac gtggagcaga caggtgatca taactggcgc tactgcatca ctgacgccta
     5581 caccggaaac acccgcaact atttaccgtg ggaggttctc caccttcgtt actccacgga
     5641 tgacggtttt atggggcgct cacctgtaac catctgccgc gaatcgctgg ggcttgggct
     5701 ggcccaacaa cgccacggcg cgagcgtgat gcgtgatggc atgatggcgg caggggttat
     5761 cacgtcaggc gaatggctgg acggcgtgaa gggcaaacag gcattagccg cactggaacg
     5821 ctacaaaggg gccagaaacg ccggaaaaac gcccatcctt gaagggggga tgagctatca
     5881 gcagctgggc atgagtaatc aggatgccga atggctggcc tcccgtcgct tcaccattga
     5941 agacatcgcc cgaatgttca acgtctcgcc gatttttttg caggaataca gcaacagcac
     6001 ctacagcaac ttcagcgagg caagccgcgc atttctcacc atgacgatgc gcccgtggct
     6061 ggcgaacttt gagcagcaga taaaaaacgc cttgctggtg gcctcgcctg tacctggtat
     6121 ccggtatcag gtggagtttg acagcgcgga cctgttacgg gccacacctg gcgaacgctt
     6181 tgccacctat gagcgcggca taaaatccga cgttatgtgt ccgaacgaag cccgcgaacg
     6241 tgaagggctg tcaccgcgtg atggtggtga tgagttcagc caggcatgga aacaggaagt
     6301 aaaaatcagc gagggagaaa aaccggaatg aacatagggc gactgcgtga caggataacg
     6361 attcagaccc tgaaacagac cagggatata accggcgaaa tactcgaaac gtgggaggac
     6421 ggtcacacac tctgggcaag cgtgaacatg gtcagcagca aggaggccat ttcatcgggc
     6481 gcagagctgg cgattggtac cgtaaggatc tggatacggt accggaagga catcaacgcc
     6541 accagccgga taaaggtcag tacggggccg ctggcggggc gtgtactgaa tatcatcggg
     6601 cagccgctgc cggatgccgc caggacacgc cttgaaattc tttgtcgtga gggcgcggaa
     6661 aaatgacaga agaacttatc accctggaag aagtgaaact ccattgccgc atcgatggcg
     6721 acgatgaaga ccagttaatc agcggataca ttgccgcatc gcttgaggcg tgccagatac
     6781 acataggcag gcgctttgat gacgggctgg cgttcacgcc agccataaag attggctgca
     6841 tgatgtttat cgctcacctg tatgaaaatc gccagattgt cgcggataac gcaaaaacac
     6901 gcgtacccat gacgataggc gcactctgga cggcttaccg tgatgtgggg gggtactgat
     6961 gccgtggcaa ccattaagac gatgcacaga accaggctgt aacaggcgcg ttaaatccgg
     7021 caagtgtgag gagcacaggc gggcggcatg gcgtgcagag gatgccagac gaggacaccg
     7081 ccgcgcgcgt ggatattcca gacagtggga caaataccgc gccatgtatc tgagtaaaaa
     7141 cccgttatgc gtgcgatgcc ttgagaaggg gatatatacg cccgccgtgg tggtggatca
     7201 cattatcccg attaatggcg gtgatgatgt tctcttctgg cccgaatgga accatcaacc
     7261 attgtgccag gcgtgtcata accagaaaac gaaatggctt gatccggcaa caaaaagcaa
     7321 gcgtgccgca ggtggatttc gtgaagagga agaacgggct gctaaccgca ataactggat
     7381 gtatgacgct gatgaatgag cgggaacaaa accgccttat ccgtggactt ataaggcagc
     7441 gtgacgcatg gaagacacag gaaacagggc ataaagataa agcgtcagga cgcgcagaac
     7501 gcatcacagc gaagcgatta accgaccgtg accgcgaggt tatggaatgt ttccgcaatc
     7561 gctgatgagg ctgtatgacg gggtgggggg cgttttcagg acaaacccga ccccgcctac
     7621 ctggatgata tcgccgtgaa gcagtggcgg gaaaaatcgc ggcagcttgc ggaacaggga
     7681 gacctgaccc ccgccgactg gagcaatctg gaactgtatt gcgtcaacta ctccatttac
     7741 cggaaagccg ttgcagacct tgcggcgcgc gggttcagca ttgttaacag tcagggcggc
     7801 gagagcagaa accccgcatt aagcgcaaaa tccgacgctg aaagagtgat gataaaaatg
     7861 gcctccttgc tcggttttga cccgataagc cgccgtaaaa atccaccgta aacagaagaa
     7921 gaggacgagc ttgaccgcct ggaataagta cgcagaagac gtaaaaacgg gcaaaattcc
     7981 ggcctgtaaa cggttaaaac aggctgttaa acggtacttt tcggacctta aaagccccct
     8041 ttacacgttc gatcgtgagg ttgtggagcg gtttattgcc ttttccaggg tgtgcccgca
     8101 cgtaaaagga ccgatgcgtg gcagacccat tgagctggag ccgtggcagc agttcgcctt
     8161 tgcgtgcatc ctcggcttta aggttaaggc caccggacgg cgcaaataca cgagcgcctt
     8221 tatcgaagtg ccgcgcaaaa atgcgaaatc cacgaccgcc gcgattctgg ctaactggtt
     8281 tctgattatg gagaacggtc agcaggatat ctacaccgca gcggtgagcc gtgaccaggc
     8341 gcggatcgtg tttgataatg cgcgtcagat gtgcctttta tcccgaccgt tacgcaggcg
     8401 ggtgaatatt caggcgcata aggtgataca cccgaaaagc aacagcctgt taaagccgct
     8461 ggcagcaaaa gcggcaacca tcgaggggac aaaccccagt ctttccatcg tggacgaata
     8521 ccacctacac cccgataacg gcgtttattc cgcgcttgag ctggggatgg gcgcacgtcc
     8581 cgaagggctg ttatttgcca ttaccacatc gggcagtaac gtcgtttcag cctgtaaaca
     8641 gcactatgat tattgctgcc agatcctgga cggcgaagag gtcaacgatt caatttttgt
     8701 actgatttac gaactggacg acgaaaacga ggttgatgat ccggcgatgt ggataaaggc
     8761 gaaccctaat attgatgttt ccgtggatcg tgaaaaactg gcctcaacca tccagaaagc
     8821 gcggggtatt ccgtcgcagt gggtggagat gatgacaaag cgatttaata tctggtgtca
     8881 ggggactacc ccgtggatgg gtaatggtgc atgggctgag tgtgccggaa cgttcacgga
     8941 ggaagattta cacggtcagg agtgttacgc ggggctggac ttatcatcaa ccagcgatat
     9001 ttccagcgta tgctatgcct ttccggtcgg taaaaccatt atgctgattt cccgtcatta
     9061 tctgccggag ttccagctac agaaccccgc caataaaaac cgcgctgtct atcgccagtg
     9121 ggctaaagcg ggctggatac gcacaacgcc aggtgactgc attgattacg accggatcag
     9181 agatgacatc atgcaggatg cggagaaatt taatatcagg ctggtgggct ttgatacgtg
     9241 gaacgccacg catctcagga cgcaattaca gggggctggt tttgaggtgg agccgttccc
     9301 gcaaacctac ctcagattca gtccggcggc gaaatcgttc gaagtttttg ttaaccgcag
     9361 ggtgattgtg catcgtggcg atccggtgtt gtcctggtcg atgagtaacg tcgtgatgca
     9421 gagtgacgcg aacgccaata tcaagccgaa caagaaaaaa tcaccgaaca agatagaccc
     9481 gagcgtagcg gcgctgatgg cgtttggtac attccaggca gagcatgagg attttgcttt
     9541 tgatataagc gacagccacc gccagaaact ggaagaattt agcggggtgt aatgaggtca
     9601 gcagcatgac agaagccgaa atactgcgat taatccgccg cgtctctgga atcagccagc
     9661 aggctgacga acagaccacg cagccggaca gcgtgacagc cgaaaattat gcgcgtgttg
     9721 ttgctgaggt gatgcgccgt gatggtatcc agcttaatga tgcggatatg cgcgacatac
     9781 ggatccgcgt tctggaaatg ctggcctaca atcgccgcgt tgaactgtat cgggagaagg
     9841 agaaaatcac gtaccactgg aagaagccgg agcggttacg gcggtaactc actgagatta
     9901 aagcaaagcg caaaattgcg ctggctacat ccctcacatc tgaggactgt taaccagcag
     9961 gccggaatat attcagaaat tacgaaattt tcgcagttta attactcgtc agcttaatcg
    10021 ctaacccgct ctgatatttt cggaaacctc aatttgagga agtcggcgag gtaactgttt
    10081 gatattcccg atagcgcaaa aatgcgcggt cgggcagaat gaccaccacc tcaatcgtga
    10141 cggagagtga caaccagtga cacaccgtga caaaaagacg gtttaaccgt tcattaaaaa
    10201 tcgacgtgat tttgtgcttt ccggatgacg tggccaacat cattttttag cgaaaaattc
    10261 tgattaaggt cactggctga acggtcataa tgaccacgca gaccatcgag gcaaccatag
    10321 cgatcatcgt tacggttgga gcttttccgc cattggtgga aaaggtgcac atctggatca
    10381 tcattacggt gatcaagggt atgtaaaacc caccagcctg attaaaggtt aaccggaaaa
    10441 aaaatccggt agccccgtta tggggatccc catatcgaca ttaacgcccc tcatgaattg
    10501 gtgcgcttcc ccctggaaag attacccgcc tgtatatttc ttgtgtctat ttgttccacg
    10561 ttgtttcata cagtgcaccg aacggtgtag ttaatggtgt agtcaatttg cgatttttgg
    10621 cactttttca atgaaaattg tttgatcgaa tcaatgactt gatgtagtgg taaaaggaca
    10681 tatttattca tctttcggcg cagaatgctg gcgaccaaaa atcacctcca tccgcgcacc
    10741 gcccagcatg ctctctccgg cgacgatttt accctcatat tgctcggtga tttcgcgggc
    10801 taccgccagc cctacacctt gcccagggcg taaagtatca acccgttgac cacggtcgaa
    10861 aatgacctct cgcttgctta atggaatacc ggggccatca tcctcgacca caatatagag
    10921 atgctcgtcg gtttgccttg cagaaatttc gacaaactcg aggcaatatt tacaggcatt
    10981 atccagcacg ttgcccatca cctcgacaaa atcgttctgc tcaccgacaa agctgatctc
    11041 tggcgaaata tcgagagaga tattgacccc tttgcgttga tacactttgt tcagcgctga
    11101 ggtgagattg tccagcagtg gggcgaccgg atgcagctcg cggctgagca atgtcccgcc
    11161 gcgcatactg gcacgatgca ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc
    11221 cagcattacc ggctcagcat cactgacgct catcttttca ctacgcagag aacgcagcgt
    11281 actttgcagc accgccagtg gcgttttcag actatgggtc aggtcggtga gcgtcgtacg
    11341 gtatttgtcg taacgttcgc gttcactttt taacaatcgg ttcaggtttc gtaccagact
    11401 ggtcagttct cgcgttgtgg ctggattgag caattcgcgg ttatgttctt ccagttcgcg
    11461 gacttctttt gccagggctt cgatggggcg taaactccac caggcggcga cccacagcag
    11521 cgggatcact aacagcagat tggctgagag cacatagata aaccagctcc agaccatata
    11581 ggaacttttt agctccaccg gaatggtatc caccaccaca atggttaatt ttggcatccg
    11641 cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc atctccgcgt cgtcatcatc
    11701 ttcccgcact tcctgcaact gttgctgtat cgaatgatct ccactcagca agaggctggt
    11761 atcgttaaca tccgcttcaa tttcatgaaa accattcgat ttcagccagt caggctggat
    11821 cattttcatc agccagggca cgtcacgttg cgcccataaa agctgcccgt tctcatcata
    11881 aattagcgtc atggtggggc tttgcttgtc gatattttcg ggtaactcga catgcaactt
    11941 attgttttcc cactgcgcaa gggtatagaa cagattgctc tcgccacgta acagccgaaa
    12001 cgtagtttta tcgaaactga cgctataacc gatcagcgcg accattccgt aggcaagcga
    12061 aagcaccagt actaccgctg ccgttgccaa cagaaaacgt acccgcagcg agagcgggaa
    12121 aaaaagacgc agtaattttt tcatcagcgc aattcgaaca ggtagccctg gccgcgaacg
    12181 gtggtaatga cttcttgcgg atattgtgcc tgaatttttt tgcgcagacg tcccatcagt
    12241 acatcaatgg tatggctttc ccgcagctcc gcatccggat agagttgtag cattaacgaa
    12301 tctttgctga ccactttgcc attattgcgt atcaacgttt ccataatggt gtattcgaac
    12361 gcggtcagtt tgatcacttc gtcattaata gataattcac ggcgagagag atcaacctga
    12421 aacgggggga gcgaaatgac ctgtgaagcc agaccgctat tacgccgcat taatgcctgc
    12481 attcgcgcca tcacctcttc aatatgaaac ggtttagtca cgtaatcatc agcaccggca
    12541 cttaatactt cgactttgtc ctgccagctt tcacgggcgg ttaataccag aatcggcagt
    12601 gaaacatcgt tgctacgcca gcggcgaatc agtgacagac cgtcctcgtc aggcaatccg
    12661 agatcgacaa tcgcaatatc cggtaaatgt tcattgagat aataatcggc ttctttggca
    12721 tcttctgcgt catcgacctg atgaccagca tcctgaatct gaactttaag gtggtgacgt
    12781 aacaacgcat tgtcttcaac aaccagtacg cgcattttta tttctccctg tcttaattat
    12841 taaaatagtg taacgcgatt atgtgtttat gggggtaaac attaaataaa ccagcgggga
    12901 ggggaggtaa agtgaaaaaa gaaaaagtgg ataatcttaa taagcaggcc ggacagcatc
    12961 gccatccggc actgatttga ggcttatttc agctcatcaa ccatcgtgat agctcgacca
    13021 atatagttag ccggcgtcat cgctttcagg cgggctttct cttcttctgg caacgccaga
    13081 ccatcgataa actgcttcat gccttcggcg tcaacgcgct taccgcgagt aagctctttc
    13141 agcttctcgt acggtttttc gatgccatag cgacgcataa ctgtctggat tggttctgcc
    13201 agcacttccc agttgtgatc cagctcatcc agcagatggt cacggttcac ttccagtttg
    13261 ctcacgcctt tcagggtgga ttgatacgca atcagcgcat aaccgatacc cacgccgagg
    13321 ttacgcagca cggtggagtc ggtcaggtca cgctgccagc gggaaaccgg cagtttgctt
    13381 gccagatgct gcaataccgc gttggaaagg cccagattcc cttcggagtt ttcgaagtcg
    13441 atcgggttaa ctttatgcgg catggtggaa gaaccaatct caccagcaat ggttttctgt
    13501 ttgaagtggt taagggcgat ataaccccag acgtcacggt caaagtcgat cagaatggtg
    13561 ttgaagcgcg caacgcaatc aaacagttcg gcaatgtagt cgtgcggttc aatctgggta
    13621 gtgtacgggt tccattgaat acccagcgag gtgacgaact cttcgctgaa ctgatgccag
    13681 tcaacttccg ggtaagcggc gatgtgggcg ttatagttac cgaccgcgcc gttgattttg
    13741 ccgaggatct ccacctggtt aagctggcgg tgctggcgct ccatacggta ggcgacgttt
    13801 gccatctctt taccgatggt tgacggcgtg gctggctgac cgtgggtacg agacagcagc
    13861 gggatatcgc ggtactgaac ggcgagatct ttaatgccat caatcagttg acgccagtac
    13921 ggcaggatca cttcatcacg cgcggttttc agcattaatg cgtgggagag gttattgata
    13981 tcttccgaag tacaggcaaa gtggatgaat tcagaaaccg cgtgcagttc cgggatctcc
    14041 gccacttttt ctttcaggaa atactcaacc gctttaacgt cgtggttagt ggtacgctcg
    14101 atggttttga tgcgtgcggc atcttcttcg ctgaaattgg cgacaattgc atcaaggtaa
    14161 ccgattgcgt cggcagcaaa agcaggaact tccttgatcg ctgcgtgcgc ggccagtttt
    14221 tgcagccaac gtacttcaac ttgtacacgg aatttcagca aaccatattc gctgaaaatc
    14281 ccgcgcagcg cgctgacttt atcgccgtag cgtccatcga caggggaaac ggcggtcagt
    14341 gaggataatt ccatagatca caactccggg gttaaatgag caagaatttg ttttgcctga
    14401 gtggtcaggc gattacgaga aaacatcagt tgtagacgtc cgccgccgac ctggtgccag
    14461 agcacggcgg cgcgaatgcc tgccagcagg gttgcgcgaa ctttcgcctg cacttgtggg
    14521 ctttgcagta cagcagggga accggtgacc tgaatgcgcg ggccaagcgg gctgatcaca
    14581 tcaacataga tagcagccat cgcgctcatc agcgtttcgg actgtaaatc gaagtgttcg
    14641 agctggcgtt gcaggccgtt gatccggttg cccagagtgt cgagcgcgcc tttcgctgag
    14701 gagagtttgc gctcaagcac catcaagctg agtgtgtagc gggttaattc ggcgtttaag
    14761 ccctggcaac tgctggcatt gagcacgccg agcagggttt ccagcccgac gcgcaggttg
    14821 gcttcgctac cgccaaaaac cgccagcgtc gagctggggt tcatatcaat aatactgttg
    14881 agtgagacgt gtagcgcatc ggcatcacaa tgcccctggt gagcgagttg ttgcaccagg
    14941 cgtgccgact gacaaatacc ggccagggcg agggtgatgt catagtaatt ctttgccacg
    15001 ttcactgctt ccttgtttta agtaaagata ataatcagac cggcagcggc agacgctgct
    15061 caataatacc gccaccgagg cacacttcac cgttatagaa gacggcagac tggcccggcg
    15121 tcacggcggc aaccggctca tcgaaaatca ctttaatgcg atcgtcgtcc agcgccttga
    15181 cggtgcacgg aatgtcggtc tggcgatagc gggtttttac cgtgcaacgc atagtgccgg
    15241 tgaatggttc gcgatcgacc cagtgcagct gctgggcaat caacccgaca gacatcagcc
    15301 gcgggtgttc atggccctga gcgacaacca gaatgttgtt ttcgacgtct ttgtccacca
    15361 cataccacgg ttcttcggtg ccttctttgg tgccaccgat acccagacct ttacgctgac
    15421 cgagagtgtg atacatcagc ccctggtgct caccaatttc atcgccatcg acggtaatga
    15481 ttttgcccgg ttgcgccggg agataacggc ccaggaactc gcggaattta cgttcgccaa
    15541 tgaagcaaat gccggtagag tcttttttct tcgcggtgac cagaccaaga tcttcagcaa
    15601 tcttacgcac ctgcggtttt tccagttcgc ctaccgggaa caggctttgc gcaatctgct
    15661 catggctgag cgtataaagg aagtagctct ggtctttatt gctgtccaga ccacgcagca
    15721 ggcggctctt gccatcgaca tcggcccgac gtacgtaatg accggtagcg atataatcgg
    15781 cacctaaatc ttcggcggca aattcgagga aggctttaaa tttgatctcc ttgttgcaca
    15841 gaatatccgg attcggcgtg cgaccggctt tatattcggc aaggaacagt tcgaagacgt
    15901 tgtcccagta ctcggcagca aagttaacgg tgtgcagttc aatgccgagc ttgtcgcaga
    15961 ccgcctgggc atcagccaga tccgctgccg ctgtgcaata ttcctcaccg tcgtcttctt
    16021 cccagttctt cataaacagg ccttcgacct gatatccctg ttgttgcaac agccaggcag
    16081 aaacggagga atcgacaccg ccggacatgc cgacgattac tttttttgcg gtttcagaca
    16141 ttggatcact cacgacattg aacttcaagg cggcgtattc tatcacgcag ccttttagat
    16201 gacacccttt gtaaaaggcc agttaaaatc gccaatcatc tccagcggat aacgttgccc
    16261 gctttgataa caacgaatac tttccgccac cagcggcgaa cgaagatttg acgcctgtaa
    16321 aatttcttcg gcgctgaccc aacggcagca gtcgatatcg ctgtcatgag gctgggtcgg
    16381 gcatatttgc tcaagctcaa tggcaaagag gaaacgcaaa aacggcgttt tatctggcgc
    16441 aatccactga tgcatacgaa taaagtgttg cggctgcgcg ctgatgccgg tttcttccca
    16501 cagttcacgg gcggcggctt ccactaaggt ttcatcggct tccagatgcc cggcaggttg
    16561 gttccataac gctttaccat taatcgtctc ttcaacgact aaaaatttgc cttctgcgtg
    16621 caccacgcaa gcaacggtaa cgtgcggttt aaacattttg catccttaat ctgtcacttc
    16681 tcgccattcg ccattggcaa gattatccaa agagtaatct cccatcgcat agcgaatcag
    16741 tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc acctggcgat tacgtccttc
    16801 atataaggtg atcttcagcc agctggtggg aatacttttg cgttcacgaa ttggtggatt
    16861 ccgcggccat aaccacgcgg gttcgtcaac cagctccgcg cctgcgggca gggtagggcc
    16921 atcatttaag gttacgccat tgcgcaaggc ttcaagtgcg tcttgtgtgg gaataccttc
    16981 cacctgcaca taatagattt ttccggtgcg tttacccggc tgggttaaac gcgcctgcaa
    17041 cgcgccgtta ttggtcagca ccagcaaccc ttcgctatcg cggtcaaggc gacctgctgc
    17101 ataaacgccc tgaaccggga tgaattcttt taatgttttg cgtccggttt catcggtgaa
    17161 ctgcggaaga acatcgtagg gtttattgaa gaggatcaca cgcgtgggct ggttttcatg
    17221 tttacgcctg gtagaacgtt gtgagctgaa tcgcttaacc tggtgatttc taaaagaagt
    17281 tttttgcatg gtattttcag agattatgaa ttgccgcatt atagcctaat aacgcgcatc
    17341 tttcatgacg gcaaacaata gggtagtatt gacaagtcaa ttacaaatca ttaacaaaaa
    17401 attgctctaa agcatccgta tcgcaggacg caaacgcata tgcaacgtgg tggcagacga
    17461 gcaaaccagt agcgctcgaa ggagaggtga atggaaagta aagtagttgt tccggcacaa
    17521 ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc ctgaaaatcc gattatccct
    17581 tacattgaag gtgatggaat cggtgtagat gtaaccccag ccatgctgaa agtggtcgac
    17641 gctgcagtcg agaaagccta taaaggcgag cgtaaaatct cctggatgga aatttacacc
    17701 ggtgaaaaat ccacacaggt ttatggtcag gatgtctggc tgcctgctga aacccttgat
    17761 ctgattcgtg aatatcgcgt tgccattaaa ggcccgctga ccactccggt tggtggcggt
    17821 attcgttctc tcaacgttgc cctgcgtcag gaactggatc tctacatctg cctgcgtccg
    17881 gtacgttact atcagggcac tccaagcccg gttaaacacc ctgaactgac cgatatggtt
    17941 atcttccgtg aaaactcgga agacatttat gcgggtatcg aatggaaagc tgactctgcc
    18001 gacgccgaga aagtgattaa attcctgcgt gaagagatgg gcgtgaagaa aattcgcttc
    18061 ccggaacatt gcggtatcgg tattaagccg tgttctgaag aaggcaccaa acgtctggtt
    18121 cgtgcagcga tcgaatacgc aattgctaac gatcgtgact ctgtgaccct ggtgcacaaa
    18181 ggcaacatca tgaagttcac cgaaggcgcg tttaaagact ggggctacca gctggcgcgt
    18241 gaagaatt
//
LOCUS       DAJTUN010000084        18204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTUN010000084 DAJTUN010000000
VERSION     DAJTUN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.084"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..684
                     /gene="kdpA"
                     /locus_tag="OO470_002660"
     CDS             <1..684
                     /gene="kdpA"
                     /locus_tag="OO470_002660"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCR6251864.1"
                     /translation="VSSLFAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFG
                     GVGSGLYGMMLFVLLAVFIAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGA
                     ALAMMTDAGRSAMLNPSPHGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMF
                     VGRFGVIIPVMAIAGSLVSKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALA
                     LGPVAEYLS"
     gene            707..2754
                     /gene="kdpB"
                     /locus_tag="OO470_002661"
                     /pseudo
     CDS             707..2754
                     /gene="kdpB"
                     /locus_tag="OO470_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            2763..3335
                     /gene="kdpC"
                     /locus_tag="OO470_002662"
     CDS             2763..3335
                     /gene="kdpC"
                     /locus_tag="OO470_002662"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6251865.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            3328..6008
                     /gene="kdpD"
                     /locus_tag="OO470_002663"
                     /pseudo
     CDS             3328..6008
                     /gene="kdpD"
                     /locus_tag="OO470_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            6005..6682
                     /gene="kdpE"
                     /locus_tag="OO470_002664"
     CDS             6005..6682
                     /gene="kdpE"
                     /locus_tag="OO470_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6251866.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            6957..7061
                     /locus_tag="OO470_002665"
     CDS             6957..7061
                     /locus_tag="OO470_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6251867.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            7372..9569
                     /gene="speF"
                     /locus_tag="OO470_002666"
                     /pseudo
     CDS             7372..9569
                     /gene="speF"
                     /locus_tag="OO470_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            9566..10885
                     /gene="potE"
                     /locus_tag="OO470_002667"
     CDS             9566..10885
                     /gene="potE"
                     /locus_tag="OO470_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6251868.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(10941..12581)
                     /gene="pgm"
                     /locus_tag="OO470_002668"
     CDS             complement(10941..12581)
                     /gene="pgm"
                     /locus_tag="OO470_002668"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6251869.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(12607..13152)
                     /gene="seqA"
                     /locus_tag="OO470_002669"
     CDS             complement(12607..13152)
                     /gene="seqA"
                     /locus_tag="OO470_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6251870.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            13337..14101
                     /gene="ybfF"
                     /locus_tag="OO470_002670"
     CDS             13337..14101
                     /gene="ybfF"
                     /locus_tag="OO470_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6251871.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            14241..14534
                     /gene="ybfE"
                     /locus_tag="OO470_002671"
     CDS             14241..14534
                     /gene="ybfE"
                     /locus_tag="OO470_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6251872.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            14674..15204
                     /gene="fldA"
                     /locus_tag="OO470_002672"
     CDS             14674..15204
                     /gene="fldA"
                     /locus_tag="OO470_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6251873.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            15414..15500
                     /gene="uof"
                     /locus_tag="OO470_002673"
     CDS             15414..15500
                     /gene="uof"
                     /locus_tag="OO470_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6251874.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            15493..15939
                     /gene="fur"
                     /locus_tag="OO470_002674"
     CDS             15493..15939
                     /gene="fur"
                     /locus_tag="OO470_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6251875.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(16023..16349)
                     /locus_tag="OO470_002675"
     CDS             complement(16023..16349)
                     /locus_tag="OO470_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251876.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(16386..17792)
                     /gene="chiP"
                     /locus_tag="OO470_002676"
     CDS             complement(16386..17792)
                     /gene="chiP"
                     /locus_tag="OO470_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6251877.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4213 a         4590 c         4862 g         4539 t
ORIGIN      
        1 gtcagtagcc tgtttgcggt cgtgactacg gcggcgtcct gtggcgcggt gattgcgatg
       61 catgattcgt ttaccgctct cggcggcatg gtgccgatgt ggctaatgca aattggtgaa
      121 gtggtgttcg gcggtgtcgg ttctggtctt tacggcatga tgctgtttgt cctgctggcg
      181 gtgtttattg ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatagac
      241 gtacgcgaga tgaaactgac tgcactggcg attctggtta ctccaacact ggtactgatg
      301 ggcgcggcgt tggcgatgat gaccgacgcc ggacgtagcg ccatgcttaa ccctagcccg
      361 catggtttta gcgaagtgct gtacgccgtg tcgtccgccg ctaacaacaa cggcagcgcc
      421 tttgccggat taagcgccaa ctctccgttc tggaactgtt tactggcgtt ctgcatgttt
      481 gtcggtcgct tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa
      541 aagagccaac ccgccagctc cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg
      601 ttaatcggca ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt
      661 ccggtggcgg aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca
      721 actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt
      781 aaacccgcag gcgcaatggc gcaatccggt gatgtttatc gtctggatcg gcagtctgct
      841 gaccacctgt attagcatcg cgatggcaag cggtgcgatg cctggcaatg cgctgtttag
      901 cgcggcaatt agcggctggc tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct
      961 ggcagaaggc cgcagtaaag cgcaggccaa cagtcttaaa ggggtgaaaa aaactgcctt
     1021 tgcccgcaag ctgcgtgagc cgaaatatgg cgctgcggcg gacaaagttc ctgccgatca
     1081 actgcgtaaa ggtgacatcg tgctggtaga agctggcgat attatcccct gcgatggtga
     1141 agtcattgaa ggcggcgcat cggtcgatga aagcgccatc accggggaat cggcaccggt
     1201 gatccgtgaa tccggcggcg attttgcctc cgtcaccggc ggcacgcgta ttctttctga
     1261 ctggctggtg attgagtgta gcgttaaccc cggtgagaca tttctggatc ggatgatcgc
     1321 gatggtggaa ggcgcacagc gacgcaaaac accgaacgag attgccctga ctatcctgct
     1381 gattgctctg accatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg
     1441 gggcggtaat gccgtgagtg taacggtact ggtggcgctg ctggtctgtc tgatcccaac
     1501 cactattggt ggcctgttgt cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc
     1561 gaatgtgatt gccaccagcg gacgtgcagt tgaagcggca ggtgacgttg acgttctgct
     1621 actggataaa accggcacca tcacactcgg taaccgtcag gcgtcggagt ttatccccgc
     1681 gcagggcgtg gatgaaaaaa cgctggctga cgccgcacaa ctggcttcgc tggctgatga
     1741 aacgccggaa ggtcgcagta ttgtgatcct cgccaagcaa cgttttaacc tgcgcgagcg
     1801 cgatgtgcag tcgctccatg ccacctttgt accgtttact gcgcaaagcc ggatgagcgg
     1861 gatcaacatc gacaaccgca tgatccgtaa aggttctgtc gatgccattc gccgccatat
     1921 tgaggctaac ggcggtcact tccctgccga tgttgatcaa aaagtcgatc aggttgcgcg
     1981 tcaaggagcc acgctgctgg tggtggtgga aggttctcgt gtgctgggcg ttattgcgct
     2041 gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcc cagctgcgca aatgggcatt
     2101 aaaacggtga tgattaccgg cgataaccgt ctgactgccg ccgcgatagc tgcggaagcg
     2161 ggtgtcgatg attttctcgc cgaagcgaca ccggaggcca agctggcatt gatccgtcag
     2221 tatcaggcgg aaggtcgttt ggtagcgatg accggcgaca gcaccaacga tgctacggcg
     2281 ctggcgcagg cagatgtcgc ggtggcgatg aactccggca cccaggcggc taaagaggcg
     2341 ggcaatatgg tggatctcga ctctaacccg accaagttga tcgaggtggt gcacattggc
     2401 aaacagatgc tgatgacccg tggctcgctg accaccttca gcattgccaa cgatgtggcg
     2461 aaatacttcg ccattattcc ggcggcgttc gcggcaacat atccgcagtt aaatgcgctg
     2521 aacatcatgc gcctgcattc gcccgactcc gccattctca gtgcggtcat tttcaacgcc
     2581 ttgattatcg tctttttgat tcctctggcg ttaaaaggcg tgagttataa accgcttacc
     2641 gcttctgcca tgttgcgccg taacttatgg atttacggtc tgggtgggct gctggtgccg
     2701 tttattggta tcaaagtcat tgatttactg ctaaccgttt gtagtctggt gtgaggttta
     2761 ccatgagtgg attacgtccg gcattatcga catttctctt tctgttattg attactggcg
     2821 gcgtttaccc gctgctgacc accgcactgg ggcaatggtg gtatccctgg caggccaacg
     2881 gttcgttgat tcgtgaaggt gatacggtgc gcggttcggc attaatcggg caaaatttta
     2941 ccggcaacgg ctattttcat ggtcgcccgt cggcaacagc agaaatgccc tataatccac
     3001 aggcttctgg cgggagcaat ctggcagtca gtaacccgga gctggataaa cagatagccg
     3061 cacgcgttgc cgcattacgg gccgctaacc cggacgccag cacgaacgtt ccggttgaac
     3121 tggtgacggc gtcggcaagc ggactggaca ataacatcac cccgcaagcg gcggcctggc
     3181 agatcccacg catcgcaaaa gcgcgtaatc tcagcgttga acagctcacg cagctgatcg
     3241 caaaatacag ccaacaaccg ctggtgaaat atatcggcca gccggtcgtc aacattgtcg
     3301 aactcaatct ggcactggat aagcttgatg aataacgaac ccttacgtcc cgaccccgac
     3361 cgtctgctgg aacaaactgc cgcgccgcat cgggggaagc tgaaagtttt cttcagtgcc
     3421 tgtgcaggcg tcgggaagac ctgggcgatg ctggcagaag cccagcgact gcgggcgcaa
     3481 gggctggata ttgtggttgg tgtggtagaa acccatgggc ggaaagatac cgccgccatg
     3541 cttgaagggc tggctgttct gcctctgaaa cgccaggcgt accgtgggcg gcatatcagc
     3601 gagtttgatc tcgatgccgc cctcgcccgc cgtccggcgc tgatcttaat ggacgaactg
     3661 gcgcacagta atgcgccagg ttctcgtcat cctaaacgct ggcaggatat cgaagaactg
     3721 ctggaagctg gcattgatgt tttcacaacc gtcaacgttc agcatctgga aagtctgaat
     3781 gatgtggtca gcggcgtcac cggaattcag gtacgggaaa ccgtgcccga tccttttttc
     3841 gatgccgccg acgacgtggt gctggtggac ttgcccccgg acgatctgcg ccagcggctg
     3901 aaagaaggca aagtctatat tgccgggcag gcggagcgcg ccattgaaca ttttttccgc
     3961 aaaggcaatc tgatcgccct gcgcgaactg gcactgcgcc gtactgccga tcgcgttgat
     4021 gagcaaatgc gcgcctggcg ggggcatcct ggcgaagaga aagtctggca cacgcgcgac
     4081 gcgatccttt tatgcatcgg ccataacacc ggcagcgaaa actggtccgc gcggcggcgc
     4141 ggctgacgtc acggctgggg agcgtctggc acgcggtgta tgttgaaacc cctgccctgc
     4201 atcgcttacc ggagaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc
     4261 tgggcgcgga gacggcaaca ctttctgatc cagcggaaga gaaagcggta gtgcgttatg
     4321 cccgtgaaca taatctcggc aagattattc tcggtcgccc agcctcgcgc cgctggtggc
     4381 gtcgggaaac atttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg
     4441 tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc gccggatagc cgctcattta
     4501 aagacaagtg gcgggtacag attcagggat gcgtggttgc cgccgcgtta tgcgccgtta
     4561 tcaccttaat tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt
     4621 atctgcttgg cgtggtggtg gcgctatttt atggacgctg gccttcggtg gttgccaccg
     4681 tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgccgtct
     4741 ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga
     4801 accttactgc tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac
     4861 ggcacttata tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag gacatcgccg
     4921 ccaccagcga acaatttatt gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg
     4981 atgacaacgg taaattgcag ccgttaacac atccgcaagg agttacgcca tgggacgatg
     5041 ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc gacacgttac
     5101 ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac gggctggtgg
     5161 tggtggagcc ggggaatctg cgccagttga tgatcccgga acagcagcgc ctgctggaga
     5221 cttttacgct gttagtcgcc aatgcccttg agcggctgac gctaaccgcc agcgaagaac
     5281 aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc
     5341 atgatttacg cacgccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc
     5401 tggcaagcga aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga
     5461 acactacccg actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta
     5521 atttgaagaa agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgccgg
     5581 aaccgggttt atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg
     5641 ttgacgggct actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg
     5701 cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat ctacaactgg
     5761 atgtctggga taacggcccc ggtcttccgc caggccagga gcagacgata tttgataagt
     5821 ttgctcgcgg gaataaagag tcggcggtgc cgggtgtcgg gcttgggttg gcaatttgtc
     5881 gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg gaaggtggtg
     5941 cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagac tttcatgagg
     6001 atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac
     6061 ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac gcggcttgct
     6121 ggaagcggca acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga
     6181 tgggattgag tttatccgcg acctgcgcca gtggagcccg gtgccggtga ttgtgctttc
     6241 cgcacgcagc gaagagagcg acaagatcgc cgcgctggat gccggagcgg atgattatct
     6301 gagtaagccg tttggtattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca
     6361 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     6421 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     6481 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     6541 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     6601 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     6661 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     6721 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     6781 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     6841 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     6901 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     6961 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     7021 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     7081 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     7141 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     7201 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     7261 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     7321 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     7381 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     7441 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     7501 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     7561 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     7621 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     7681 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     7741 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     7801 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     7861 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     7921 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
     7981 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
     8041 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
     8101 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
     8161 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
     8221 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
     8281 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
     8341 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
     8401 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
     8461 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
     8521 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
     8581 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
     8641 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
     8701 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
     8761 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
     8821 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
     8881 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
     8941 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta actccatcct cttcctgctg
     9001 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
     9061 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
     9121 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
     9181 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
     9241 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
     9301 gtctgccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
     9361 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
     9421 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
     9481 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
     9541 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
     9601 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
     9661 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
     9721 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
     9781 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
     9841 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
     9901 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
     9961 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    10021 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    10081 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    10141 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    10201 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    10261 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    10321 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    10381 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    10441 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    10501 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    10561 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    10621 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    10681 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    10741 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    10801 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    10861 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    10921 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    10981 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    11041 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    11101 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    11161 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    11221 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    11281 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    11341 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    11401 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    11461 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    11521 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    11581 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    11641 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    11701 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    11761 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    11821 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    11881 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    11941 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    12001 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccctgtct
    12061 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    12121 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    12181 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    12241 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    12301 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    12361 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    12421 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    12481 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    12541 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    12601 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    12661 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    12721 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    12781 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    12841 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    12901 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    12961 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    13021 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    13081 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    13141 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    13201 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    13261 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    13321 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    13381 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    13441 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    13501 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    13561 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    13621 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    13681 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    13741 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    13801 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    13861 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    13921 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    13981 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    14041 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    14101 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    14161 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    14221 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    14281 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    14341 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    14401 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    14461 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    14521 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    14581 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    14641 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    14701 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    14761 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    14821 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    14881 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    14941 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    15001 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    15061 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    15121 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    15181 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    15241 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctgta
    15301 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    15361 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    15421 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    15481 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    15541 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    15601 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    15661 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    15721 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    15781 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    15841 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    15901 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    15961 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    16021 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    16081 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    16141 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    16201 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    16261 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    16321 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    16381 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    16441 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    16501 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    16561 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    16621 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    16681 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    16741 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    16801 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    16861 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    16921 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    16981 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    17041 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    17101 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    17161 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    17221 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    17281 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    17341 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    17401 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    17461 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    17521 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    17581 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    17641 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    17701 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    17761 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    17821 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    17881 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    17941 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    18001 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    18061 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    18121 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    18181 tttgctattt cttgccggat gcgg
//
LOCUS       DAJTUN010000085        18130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTUN010000085 DAJTUN010000000
VERSION     DAJTUN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.085"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            475..909
                     /locus_tag="OO470_002677"
     CDS             475..909
                     /locus_tag="OO470_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCR6251878.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            1002..1268
                     /locus_tag="OO470_002678"
     CDS             1002..1268
                     /locus_tag="OO470_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251879.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1445..1834
                     /locus_tag="OO470_002679"
     CDS             1445..1834
                     /locus_tag="OO470_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HCR6251880.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            1831..2757
                     /locus_tag="OO470_002680"
     CDS             1831..2757
                     /locus_tag="OO470_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6251881.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            2757..3002
                     /locus_tag="OO470_002681"
     CDS             2757..3002
                     /locus_tag="OO470_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251882.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPLERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(3033..3284)
                     /locus_tag="OO470_002682"
     CDS             complement(3033..3284)
                     /locus_tag="OO470_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6251883.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            3865..4712
                     /locus_tag="OO470_002683"
                     /pseudo
     CDS             3865..4712
                     /locus_tag="OO470_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(4797..5132)
                     /locus_tag="OO470_002684"
     CDS             complement(4797..5132)
                     /locus_tag="OO470_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCR6251884.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            5365..5697
                     /locus_tag="OO470_002685"
     CDS             5365..5697
                     /locus_tag="OO470_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6251885.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            5709..8420
                     /locus_tag="OO470_002686"
     CDS             5709..8420
                     /locus_tag="OO470_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR6251886.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            8488..8919
                     /locus_tag="OO470_002687"
     CDS             8488..8919
                     /locus_tag="OO470_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HCR6251887.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            9149..9418
                     /locus_tag="OO470_002688"
                     /pseudo
     CDS             9149..9418
                     /locus_tag="OO470_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(9525..10361)
                     /locus_tag="OO470_002689"
     CDS             complement(9525..10361)
                     /locus_tag="OO470_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6251888.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(10361..11164)
                     /locus_tag="OO470_002690"
                     /note="aph(3'')-Ib"
     CDS             complement(10361..11164)
                     /locus_tag="OO470_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6251889.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(11225..12040)
                     /gene="sul2"
                     /locus_tag="OO470_002691"
     CDS             complement(11225..12040)
                     /gene="sul2"
                     /locus_tag="OO470_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6251890.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(12904..15207)
                     /locus_tag="OO470_002692"
     CDS             complement(12904..15207)
                     /locus_tag="OO470_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCR6251891.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            complement(15188..16312)
                     /locus_tag="OO470_002693"
     CDS             complement(15188..16312)
                     /locus_tag="OO470_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HCR6251892.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            complement(16309..17598)
                     /gene="trbA"
                     /locus_tag="OO470_002694"
     CDS             complement(16309..17598)
                     /gene="trbA"
                     /locus_tag="OO470_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCR6251893.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTEKQHPADKEKEQ"
     gene            17936..18082
                     /locus_tag="OO470_002695"
     CDS             17936..18082
                     /locus_tag="OO470_002695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251894.1"
                     /translation="MSGDLPSHQSIGTMDLCYSKTWPDKQVVLRLVLWPEEVSFQLTM
                     KELL"
BASE COUNT         4254 a         4479 c         5099 g         4298 t
ORIGIN      
        1 ctccgccgaa cccgctggcg cggtttcggg ctgccgctac ggtccgatgt ctgaaaaacc
       61 aggcttgcag cgaccgttcc ggtcgcgccg cctctgtcgc gccgtgacct gcgctccctg
      121 cacacctgac ggtgtgtaag gaaactgcgc ctgatggcgc agttgtctgc ccttccgccg
      181 ccccccctgc ggcctgtttc cccgctgtgg actttccggg ggctgaagcg gccacaacac
      241 gtttcagccg tccaggggag agatgtacca gccacaaaac tttttgccgc aagcgtcaaa
      301 aacttttgcg tctgcccccg gacaactgcc ccgtgccgtg gcagtcgctg ccccatcgga
      361 aaggtccact gcggggagaa accccgaaga agtggacggc agaaggggct ttcgcccctc
      421 cgggggagca accggtcggc ccggcgacct gattcagcag aaggagacgt tttcatgaca
      481 gtcagcagca cgatttccgt tttttgccgc gacggggtgt tccgtaccgt ttactgccac
      541 ctgcacggtg agccgacctg gaacggtcgc atcctgcata cccactatgc caccggtcag
      601 caggccgaag ccctggttga acacggtgat atccgttgcc tcggtccccg ttgcgacaaa
      661 cccgccgggc atacgcttca gaacccggtg gacggtgtga cggcttatta cggacgtgac
      721 agtggtttcc ggatggacag tgaggcgcgt gagtaccgtt ctttcaggga ggccatcgcc
      781 acggaaagca ctgaagaggt gcgcttccat tatgtgttca tcgacggcta ctggaaggtg
      841 atgtaccgca cgccggaagg ctggaagatg aaagcgctcg cgctggcact gcgtcgctgt
      901 ccggaatgaa aaaaagcagg gcgataaacc ctgcctttct ccgctggcgc ttccccgtac
      961 ggaagtccgg catctcattc actaactatg gagattatgc catgagacca tcaattatct
     1021 tcgcaaccgc cgagtatgta aagcgtctgc gtgaagagtg cctgcgggag aacaaacccc
     1081 tgcaccgcca tacccgcttc agacgccagg aactggcacc ggatgagatt aacccggacg
     1141 tcctggcgat gggcggtcat atcgccagac gctgcagtga gcagaagcgg gtacgtatcc
     1201 ccgccatgaa agtcagcgaa tggggccacc tgctccgcgc gcttgaaatt gaacgggtct
     1261 gtcactgatt aacccctgcc gtctgcctgt gcaggtggca ggatgtgttc aggtacttta
     1321 ccaggaggaa ccgtgagcaa gatgaaaaac atcacgacgt ctgtgttgca tggtgtctcc
     1381 acaaaaaaca gaaaagggaa acagattctc tttcattcag ggcccgggag ggctggagat
     1441 aacaatgaag tcttgttttg atactgacaa agcttttcat atgtcttttg atgaagtcac
     1501 tttcaacgct gacagaattc gctcaaaact ggaactatta tccgaagaac taatgccccg
     1561 ttgtggtctt gtttatgagc tatatcagcg acgtttgagt gctgctattg ataattttgt
     1621 ggccacattg ccgacagaac aacatctact ggtcctggaa ctggcccgtg aggagtttga
     1681 ttacctttcc gcagaagaga ttgctgaaga aatacgccgt gactcagaac gcggctattg
     1741 ttgtcatggc tttgaacgca attgctgtcc tctcggttgt ggggatttgg gcgatgattg
     1801 ctgcgatgct gctgatttga tggaggaacc gtgagtaaga agaaaaacac cacaacgccc
     1861 acgccgcatg atgccgcgtt ccggtcgttc ctggcgaatc ccgacgttgc cagggatttt
     1921 ctggaactgc atcttcctgc ggagtaccgg cagttgtgcg acctgtccac gctgaagctg
     1981 gaacccgcca cctttgttga gccggacctg catcagtacg ccagcgatat cctctggagc
     2041 gtgaaaacca ccgggggtga agatggttat gtttatacgc tcatcgagca ccagagcaca
     2101 gaaaatctgt acatgccttt tcgcatgtta cgctacagtg tggcggcgat gcagagacat
     2161 ctggagcagc acaaaacgtt gccactggtg attccggtgc tgttctatca cggtgagcgc
     2221 agcccgtacc cgtacagcat gaactggctg gactgttttg agaatccggc acttgcggct
     2281 aaaatataca caaagccgtt tccgctggtt gatatcactg tcgttgatga caatgaaatc
     2341 atgaaccatc gccggatggc cgcactgacg ctgctgatga agcatatccg ccatcgtgac
     2401 atgatggagc tgctggacaa actcccgcag gtcatggtgg aaatttcaga cgagcaggtg
     2461 cgtgttctga ttcattacat cgttaacgca ggggactctg tatcaccgga atttatgcgg
     2521 gcgctggctg agcgtctgcc gcagcatgag gataaactga tgactatcgc agaacgtctt
     2581 gagcaaaaag gtcgccagga aggcaggatg gaaggacgca tggaaggacg catggaagga
     2641 gcgcttgaaa aagccctggc tattgcgtgc cagcttcaga aaatggggat gacgccggag
     2701 cagattaagc aggctaccgg actttccgat gacgaactga agaaaatcac tcactgatga
     2761 gaaccacaaa gcccggcaaa atgccgggct tttttgtcag caggagcaga gcaaacagca
     2821 tgacgattgc agaacgtctt atacagaaag gtttcgacga gggttttaaa caaggggctc
     2881 ttgaagtggc gcgggaaata gcctgccggc ttcgggatat aggctggccg ctggaacgga
     2941 ttcaggaggt cacgggactt tccggtgaag aactgaaaaa gctgtttcct gatgagcagt
     3001 agcctggcat tcagccaggc tgtgaatcac gctcacacat tccctgccgt ttcccccagt
     3061 tcattttcca gtcgcctgat gatatctgcc ttacgcatcc cctgcatgtg aggcagcagg
     3121 tcacggactg aaagtgcctg ggtggtcctc cagacggtga cgtccacctg ttccgcattt
     3181 tcccaggcgt gccatgttct ctgcgtcacg ccaaattctc tggccgcttt ttcgcgtgac
     3241 cagagcatgc tttttcgcca gattcgcagt tcccagccgg tcataaaaca cctttgaaaa
     3301 agtgaaaaaa tttcacttta ttttatcatc ataatcacca aatcaacgcg aaaaataagc
     3361 gtgaaaaagt gaagaaattt cacttttttt tggttgtaaa tgtcgtgcgg ttttctctgt
     3421 tttcacctgt gcgggagtgg tgtgccagtg accaccacat gccattgttg tgccgtcgtt
     3481 tttcttcagt cgcgccgtat gcggcgctcc ctgcacacct tacggtgtgt aaggccactg
     3541 cgcctgatgg cgcagctgtc tctgcctgtc tgccgccgca ctgcggcctg ttctccgcag
     3601 acggactttc cgggggtaca tacatgacca catcccgttt cagtcgtcca ggggagagat
     3661 gtaccagcca caaaactttt tgccgcagcc gtcaaaaact tttgcgtctg cccccggaca
     3721 actgccccgt gacgtggtga gcgtcccccg tcggaaaggt ccactgtggg gaaatccccg
     3781 aagacccgga cggcagaagg agcttcgctc ctccgggaaa tgacctggct gacccggcag
     3841 tcgtgacgat gaggagacaa tgtgatgaat cagactttac ccactgctga cctgaatact
     3901 gccggcacga cagatgttat tccgtctgtg gctatcgacc gcatcatcgc gcagcgtaac
     3961 gaaggtattg cactgttcat gcaggcgatg gaatgcctgg cgacagcgcg caggattctg
     4021 ctcgatgctt ccggtgacat ttttctctac gggtttgaag actgcgtgac tgactccgtt
     4081 cgatgcatgg ataaaccgga agaagcaaaa aagaatatca cccgtcttgc cgaccggaaa
     4141 atctgggacc gcctgatgac agatacgggc atgtacacct tcatgagttc atgccagcgt
     4201 gatgagtgga acagccagct gatgagcgac acctgtcctg aaatcaccct ggacaatgta
     4261 ctggcaacat tccgccatct taatgccagc aagatgcaga cgtttgaaca gggacttatt
     4321 gatatctacc ggaaattgtc atgggattac agaagcaata atccctgtcg tctgggcaag
     4381 agaatcatta ttgaaaacct gctgtaccgc tggagtaacg gcgtgtgacg ctggactgca
     4441 gcggacggga ggcactggat gacctggtac gccctttttg tctgctggag gggcgcaacg
     4501 ttcctgagtt ccggagcagc atcggggcgc agtatggtga atttctcggg aacggtgaca
     4561 atgtcggtaa gctgttagaa ggggaatatt ttacggtgcg tggctaccag aaagggaccg
     4621 tacacattgt ctttaagcgt cctgaccttg ttgaaaaact gaatgatatt attgcacggc
     4681 attatcctgg tgcattgccg ccacgagtct gattaaacag aaaagcccag gtatttatgc
     4741 cggggctttt tttctgtaat tatcataatc tcagaacacg atgtgacatt gtcacatcaa
     4801 ttgttgttca tatcgatgcc atctttcctg cctgaatctg tctttttagg cgctgtttta
     4861 gaggaagaac ggcgggatgg ttttttcgat tctgcttccc gaataatgtc acgaagtgcc
     4921 ttctctccta cttcataccc tgcatttttg agcgtgtcac gaacatctgc cagtgtataa
     4981 ccctttgtcc ttaccaggac aataatgtca tcacgaatgg catcaagaaa atcacgcaat
     5041 gttttccgtt gagcggtgag gtctggcaat tccgataaag cagcttttgc cagctggatg
     5101 tcatcatctg agtaaaattt tttagaagcc attggcactt tctctccgtt tcatgttgaa
     5161 gcctgatttt atcatcaaaa actgcctcca ggcagggctg cgccctgcct ctgccgtctg
     5221 cataagacta tgatgcacaa aaataacagg ctataatggc ctgaaaaacg ggacaggatg
     5281 tgggaatgtc ggaccgcgca cgcgcactca gacattcccc acctggtcag ggctttgccc
     5341 cgacaccccg gtaaggagca gaccatgagt gagaagaaaa cccgaacggg cagtgagaac
     5401 aggaagagaa ttgtaaaatt cacggcgaga ttcacggagg atgaggctga aatcgtaaga
     5461 gaaaaagcgg agtcaagtgg acagacggtt tcaacattta tacgaagttc ttcactggat
     5521 aaggtggtta actgccgtac tgacgaccgt atgattgatg agattatgcg tcttggccgc
     5581 cttcagaagc atctgtttgt ggagggaaaa cgcactggtg ataaggagta tgcagaggtt
     5641 cttgttgcca ttactgaata tgtcaatgct ctgcgaagag acttaatggg acgctgagat
     5701 aactgctgat gaatgccatt attccgcaca aaagacgtga cggcaggtct tcctttgaag
     5761 acctgattgc gtacacgtct gtgcgggatg atgtgcagga aaatgagctc acagaagaca
     5821 gcgaaatcaa gagtgatgtc cctcaccgca accgtttcag acggcttgtt gattatgcaa
     5881 cacgcctgcg taacgaaaaa tttgtctccc tgattgatgt gatgaaggac ggcagtcagt
     5941 gggtaaattt ctatggcgta acctgtttcc ataactgcaa ttcactggaa acagctgctg
     6001 aagagatgca gtacaaggcg gacaaagctg ttttttcccg taatgataca gaccccgttt
     6061 tccactacat tctttcctgg cctgcgcatg aaagcccgcg tcctgagcag ctttttgact
     6121 gtgtgcgtca cacactgaaa tctcttgaac tctcaaaaca tcagtacgtg gctgccgttc
     6181 atacggacac ggataatctg catgtgcatg ttgccgtgaa tcgtgtacat cctgaaactg
     6241 gttatattaa ctggctttcc tggagccagg aaaaactcag ccgtgcatgt cgtgaacttg
     6301 aacttaagca cggattcgcg cctgataatg gctgctttgt gcatgcaccc ggcaaccgta
     6361 ttgtgcgcaa aacagcgctg gtacgtgagc gccgtaatgc ctggcgacgc gggaaaaagc
     6421 agacgttcag ggaatatatt gcgcagatgt cgattgccgg gctgcgtgaa gagcccgcac
     6481 aagactggtt gtctttgcat aaacggctgg ccagtgatgg tctgtatatc acgatgcagg
     6541 aaggggaact ggtggtgaag gatggctggg acagagcgcg ggaaggtgtt gcactcagtt
     6601 cgttcggacc gtcatggaca gctgaaaaac tggggcggaa actgggggaa tatcagcctg
     6661 taccgacaga tattttcagt caggtgggga cacccggtcg ttatgatccg gaagccataa
     6721 acgttgatat ccggccggaa aaagtggcag aaacggagag tctgaaacag tacgcctgcc
     6781 gtcattttgc cgaacgctta ccggaaatgg cgagaaatgg tgagctggaa agttgcctgg
     6841 atgtgcacag aactctggca aaggctggat tatggatggg gatccagcac gggcatctgg
     6901 tactgcatga tggatttgat aaacaacaga caccagtacg tgcagacagt gtgtggccgt
     6961 taatgacgct ggattatatg caggatctgg atggtggatg gcagcctgta ccgaaggata
     7021 tttttactca ggttataccc ggagaacgat tccggggacg taatcttggt actcaggctg
     7081 tcagtgatta cgaatggtat cgtattcgga tggggacagg tccacaggga gcaataaagc
     7141 gcgaactgtt ttccgataag gaaagtctgt gggggtacac gactgttcag tgtgagtcgt
     7201 tgattgaaga tatgatcgcg ggcgggaatt tcagctggca ggcgtgccat gagatgtttg
     7261 cgcgtaaagg gcttatgttg cagaaacagc atcatggtct tgtgattgtg gatgcgttta
     7321 atcatgagct cacaccagtg aaggccagca gtatccaccc ggatttgacg ttatccaggg
     7381 ctgagccgca ggcagggcca tttgagattg caggtgcaga tatatttgaa cgggtgaagc
     7441 ctgaatgtcg ctacaatccg gaacttgctg ccagcgatga agtggagctc ggcttccggc
     7501 gcgatcctgt actgcgtcgt gaacgccgtg aagcccgtgc agctgcgcgg gaagatttgc
     7561 gcgcgcgtta tcttgcatgg aaggagcact ggcgtaaacc cgacttacgt tacggagaac
     7621 ggttacggga aattcatgct gcctgccgtc ggcgcaaggc gtatatccgt gtgcagttcc
     7681 gggatccaca attgcgtaag ctgcattacc atattgcaga agtgcagcgt atgcaggcgc
     7741 tgatcaggct gaaggagagt gtgaaggaag agcggttgtc actgattgcg gagggtaagt
     7801 ggtatccttt gtcctaccgg cagtgggtgg agcaacaggc ggtacaggga gacagagctg
     7861 cattatcaca gttacgtggc tgggattatc gtgatcgtcg caaagacaag cgacgaacaa
     7921 cgaatgccga ccgctgcgtg attctctgtg aaccgggagg aactccactt tatgaagaca
     7981 ctggcgttct cgaagcccgt ctgcagaaag atggcagtgt gcgttttcgt gatcggagga
     8041 atggcgagtt agtctgtgtg gattatgggg accgtgtggt gttctaccat catcaggaca
     8101 gaaatgaact ggtggataag ctgaatctga ttgctcctgt attgtttgat cgtgagccag
     8161 gaatgggttt tgaaccggaa ggctcatatc aacagtttaa tgatgtattt gcggaaatgg
     8221 tggcctggca taatgcagct ggaataaccg gaagtggaca ctttgtaatc agccgcccgg
     8281 atgtggattt acatcgtcag cgtagtgaac agtactacca cgaatatatc aggcaacaga
     8341 aaagcatatc cggggggcat ggcgcatctt atgctccagt tcaggataat gagtggacgc
     8401 caccatcacc tggaatgtag ggatgaggag tggatattaa aaatcttaac ttgtattagt
     8461 taagattttc tttgctctga cagagctatg gtactgagat ctatatggat tgaggagcaa
     8521 tatatggggg taaagaaaat cgttatgctg gctattttag tcagctttgt ggcgggatgt
     8581 gcgccattac atccttcaaa ttgccataaa actacagccc tgggtagttg cagttcagga
     8641 cgttgggatg atcaggatga atggggggag caagcacggg gaatcaggga tgcaattaat
     8701 gctaaacttg atgatccgca aaaatggaaa aggaaaaaat gcaggttgca tatggaattc
     8761 tcccgggatg gcacggcgtt aaaaatatca accagtaatg gcgataaagc ctattgcgaa
     8821 gcgataaagt cagcagccca taaagctaaa ttcccggcct tcaacaatcc ggaagtttat
     8881 agagattttc aaaaatccgg ctttgacatg cgcggttagc tccacgacat atctgtaggg
     8941 ctaacagaag actgtcaggt tggctgatta ctacactgga acctgaggta ctttgcctca
     9001 ggttatgttg acatactcaa aaatagattc ttacattatc ccggtaacgg cgtccttcat
     9061 cggcctgcca gttaccatca atggtattgg taccctcgtt ctttctgtct gtgatgttaa
     9121 taagggcaaa attaatcagc atgtctggca gggaaaggaa ttgccgggct atgtgcaacg
     9181 ggaatttgaa gaatttctcc aatgcgggcg gctggagcat ggctttctac gggttcgctg
     9241 cgagtcttgc cacgccgagc acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc
     9301 gagctgtggg gcgcggcgga tggccgaaag tgccgccttg ctggttgatg aagtactgcc
     9361 tgaacaaccc atgcgtcagt gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg
     9421 ggtcgtttgc gggaaggggc ggaatcctac gctaaggctt tggccagcga tattctccgg
     9481 tgagattgat gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt
     9541 cgcacctgct tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg
     9601 ttccaagctg cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg
     9661 acgtccagcg cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa
     9721 aggtcgtctc tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg
     9781 accagaccga cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga
     9841 tgcagatcgc catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata
     9901 atcgccgcgt ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga
     9961 aacaaagctg caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat
    10021 gcggcataca gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc
    10081 tcggcaacga tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc
    10141 tcacgaccga gcaaccggac tgctcccctc ccattgcgcc ataccagata gtcggccccg
    10201 cgcagttcat cagcaatgtc ttctataggt ttcaatccct tgacgattgc aggagtcccg
    10261 tctggcaatg aaactttcca aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc
    10321 gaaacgtgcc aatgagcagg aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt
    10381 ccaatcgcag atagaaggca aggcgttcgc ggtcgggggc ttcgatcccc aatacattga
    10441 ataggacagc gaaggcgcgc tctgcttcat ctggcgctgc ccagttctct tcggcgttag
    10501 caatcatgag tgccaaatcg gcatagcgat ctgctgttcc gagccgccca aggtcgatca
    10561 gacccgtgca ttgaagagtt ttagggtcca ccatgaagtt cggcatgcag ggatcaccat
    10621 ggcaaacaac catatcggtg cgctcttggt cgagccgcac cggtagctct cgttcgacac
    10681 gagccaaaag atcgagctgc ggcgtactct tgtcctcgtc cggtaagaag tcgggattga
    10741 cggcattgcg ggacaccaca tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa
    10801 acggacattg atcaaccgat aggctgtgaa cagcgccaag ttgctgcccc attgacggcc
    10861 acgctttgag caaatccgct ccagacagat cagccgccgg tactcccgga attgccgtta
    10921 tcaccaagca tgcaccctcc tgttcctcct gccagttgat gacctcgggg caagccacac
    10981 ctcgaccttt gagccaaatg aggcggtcac gctctccagc gagctcaccg cggcgggaag
    11041 caggtgcgat tttcgcgaag gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt
    11101 ctccgcctct gacaggcaac cagtcagaat gcgattcacc aaaaaaaata ttagttcgat
    11161 tcaatggagg ttccttcagt tttctgatga agcgcgaata tagagaaata tcccgaatgt
    11221 gcagttaacg aattcttgcg gtttctttca gcgccgccaa taccgccagc ccgtcgcgca
    11281 aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc tgcggcggcg gcaagctctg
    11341 cagcgagtgt cgcggccccg acatcccccg gaccacggcc tgtgagcgcg cgcagaaagg
    11401 atttgcgcga aacagacaga agcaccggca aatcgaagcg cagccgcaat tcatcgaacc
    11461 gcgccagcac cgagagcgag gtttcgggag cagcccccag aaaaaacccc atgccgggat
    11521 caaggacaag gcggttgcgt ttgataccgg cacccgtcag cgccgcgatg cgcgcgtcaa
    11581 agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc ctcgcgccga tctgcctgcc
    11641 cgtcttgcac cgaatgcata acgacgagtt tggcagatga tttcgccaat tgcggataga
    11701 acgcagcgtc tggaaaaccg cgaatatcat tgagataggc cacaccacgc gacaaggcat
    11761 aggcttgcgt cgcgggttga taactgtcga gcgagacggg aatgccatct gccttgagcg
    11821 cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga cgaaacaggc gcggcgtcgg
    11881 gattgctgga tgccggaccg aggtcgatca catctgcccc ctcggccatc agcttacgcg
    11941 cctgcgcaat ggctgcgtct ggcgccagat accggcctcc atcggagaaa ctgtccgagg
    12001 ttatgttgac gatgccgaaa atgatgagcg atttattcat gggggcttct ataataataa
    12061 taatcgagca tgagtctcat acggatgctc gggtcgaaag ggaatcccca ggcgagtaac
    12121 ctgtttgcgg tgatccatta gctgcaggag cagaatagca tacatctgga agcaaagcca
    12181 ggaaagcggc ctatggagct gtgcggcagc gctcagtagg caatttttca aaatattgtt
    12241 aagccttttc tgagcatggt atttttcatg gtattaccaa ttagcaggaa aataagccat
    12301 tgaatataaa agataaaaat gtcttgttta caatagagtg ggatctggga attcatacca
    12361 atatccatag aagtgaatct attacgaacg tggggaaccc tggcaccatt ttttgtgctg
    12421 cccatatttg ttttcatgta taattcataa tcaaagcgtt catcctgatt tagcttaact
    12481 gattaaaatc cgtagattat gattttatca gttttgtgac tttatttatt atggggagct
    12541 tacccagtat ttcgaaatta tcgggagaac acttgtacca tctgtttgag cctgcaagta
    12601 tttctctgtc ccatcgtctg accctctccc cgtttctgag tatgcatatc atatagtttt
    12661 cgggcaccat cagtgtccgg gctgccagaa gactattttt aaacgtcagg gaagtgatat
    12721 tgatatcttc tccggaattt ttacgataaa taacagacca tgagtttgtt tccgggctac
    12781 taatacacat ttattgtacc tctggtgatg gattgaaatc ctgaatcctc cgcttactat
    12841 ggtgagcgga ggagattaat aatcgcgtaa aggttgttat taaaatcagc catacaggtg
    12901 ttatcagaat ctttttttac gactctcttg ttttccacct gatggtgtct gagcgacatt
    12961 ttttttatgg ggcggggatg cagttagatc cggtacaggt aacagcatct ccagggggag
    13021 cagagtattc agaatcgcct tcactgaagg tatatcttca ggaccggagt gttcctcttc
    13081 cggacgattc agtgtgatat aacatacccg gcgttcttcc ctggttgtgt tattgatagt
    13141 gtgcacagcc tttttccaca gttcgtttgc ccgggattcg atatctgtat catattgcgg
    13201 aagcagatcc agtgatgtct gcgctgttgc caactggcgc tggaatgtat caatgatacg
    13261 gtaaggctct gtacggtttt tacgacgttt acgggatggt tttgctgtca gtacaccgat
    13321 gatgctgctg acatgctccg gtgtggggag cctgcgctgc actgtcctgg ggacgagacg
    13381 gcggagacgc tccagccggg gggcttcaac ggaaatatag cggttgatga ccaccggatc
    13441 actgtcgcaa cttttctcgc tgtccggaat atacacagag gctgacggca ccacatcccc
    13501 ctggaaaacg gtaaagaact caccagcatt cagtctgatc aaatcctgca cgtcgatttt
    13561 gctttccatc tggatggtga tctggttacc gtccacccag gactcaccca tcacaccatc
    13621 gtggcgctgc aggctgccca ttctggcccg tgcctcttca ccggcggcat ttttcaccgt
    13681 tcttgccgtt ttgtcgtcac tgacaatgcg tccggcgaat ttggtgccgg cgttctgcat
    13741 cagtgtggcg gtgttggtgg cggaggtctg cccttcaatt cgctcctgat cctgcgccgt
    13801 cattatcagg gaaaactcca gtgaacgtac ctgggtggcc tccacagcaa tgcggtctgt
    13861 gtagtaggca ccgacttcgt caaggataca gatatagggg aagctgcctt tgtatttttt
    13921 gacttccagg gtctgtgcat ctgtgccttc cagccggtag cccaggtcac gggcgaggag
    13981 catgctctgc tgggtgacga acatacgacc gagtgccgac gtggtatgag ccgatgtgtc
    14041 cagtgcaggg atcatcacaa tcagaatgcg gtcgctgtga atactgtcgc gaatatcgat
    14101 gtcgccggca tctgccgcaa agacgtcccc gaaggtttcg gcaaaggttg tgaacgtttc
    14161 cgagaactga ccggagagat aagcatgctg tttacggggc tcctcagtcc aggctgacgg
    14221 cgttctcacc agtgacatat cgaatcccgg gacatcctgc aggtagtttc gcagtggtgc
    14281 gatagcttct tccggccact ggtcatcaag gccacggcaa tagagttttg ccatcccttc
    14341 cagcgtcata tgttcccgta acatctgcag ggacatggtt ttaccttccc ggacgcacca
    14401 gaattttgtg ccaaagacca gggccttgtt catggctatc gcccgtgact gccattcacc
    14461 gccctgaacg ttctggggca gcatggactg catggtttcg gcggtgaagg cttcggtgct
    14521 gaatgcgaac gggttccagg tgtttgactg tgggcgggat ttttcgccgc tctgaatgat
    14581 ttcactgcgg gattttccgc cgttcatgaa gttgatgact tcgatatcat cctccctgcc
    14641 aaaacgtcgg gagagatacc agattgtcct cgtggtatcg ttctgtgcct ttccgtcaac
    14701 aaaggtaaag cctcttcccc agcagagcgg gttgagcagc caggcaaagg tggtttctgt
    14761 tttaccgcca ccggtggtgg caaaaaacat gacatgccgg gtgaggtcat ccatgctcag
    14821 ccagagctca cgaccgatat cgttgatacg gcggtaaccg acataaaaaa tcccccgggc
    14881 ggggaatgtt tcatccgctt catactgaaa cagggtgggc cagaagctga agaggcttct
    14941 cctgaccttt ctgtcctggg aggggtcatc aaggctgagt gtcatgggca tacgcagggg
    15001 gcatcgccag cgttgcagca tgaatatcac ggaggtgacc agggtgaaca gcaggcagac
    15061 aggaagggcc agtggccata tcagcgccgt aatgatactg ccggccagac acagaccagt
    15121 gatgttcagg ttatgcagtg cattccacaa cgggtttccc caggccgtgc ggtgtatgag
    15181 gtcaggatta acgtggtgct ggctcattgt gatactcctc gctatctgtt gctttcagga
    15241 agaggcgaac ggaagtggcg tctttcagga tgccggacgg caggtgccag ccggtatctg
    15301 ataacaccca gggggttccg ccaaaaccga actggcggaa agcctggtta ttgacatcga
    15361 tggcagcacg tgcggcggtc agacactgtc catcctgtgc ggccggtgca tcgccggtgg
    15421 gggccatacc actgtcagcg gaatacagct gatgccatcc ggtcgcccgt ttttgtgagt
    15481 ccggttcaca cagcagcgga gcagtccgct ttgtactgga ctccccgcca atcacggaga
    15541 ccggataaat caccacgctg aattcccggg ccagttgctt aagcgcgggt tccagcagac
    15601 ggcagttgat gcatgcgggg tcaaggaagg cgtacagcgt gcgttcatga ccagtggaca
    15661 ggttaatggt gaacatcccc cggtctgcgt tgcgtttaag tactgtggcc agattacggc
    15721 gggcctcagc ggcctgaacg tgttgttctt ccgtcactgc cgtcggggcg ggggcagaag
    15781 aaggttcctg ctgtgtggga gccgggcggg ccgggatgct gttaacatga tgttccgtat
    15841 gtgggcgtgg tgtctctgtg tcggttactg gttcagctct ccattcctga gatgtagggt
    15901 aagccagcgt ggaagggggc tctgccttca tccagtggta acctgcaact gtcgccggca
    15961 gagcaattcc ggcgataaag ccacacatca gtgtccgcca gaagccattt ttccgggagg
    16021 gatgttttcc ttttccggca tattcggcat ccaggcaggc atcaatcgct gagagcattc
    16081 tgccgatgtg atgtccggga cgttctgcat caacggtatc aacaagcacc gtttcggtta
    16141 tttcgcgaaa aggggaactg aaggacaggt gcatcagcct gttattacgg ttaatcagtg
    16201 cgcccagttc tgtgactggc aggatggcaa tcacctcccg attaccgtca gggtacaggg
    16261 cgatcaggga caggtgttta ttattcagtg ttgcagtgta gtggatggtc attgttcttt
    16321 ttccttgtcg gcgggatgct gtttttcggt ggtttcggtg gtttcggtgg tttcggtggt
    16381 ttcaccagtg tcagtgtctt cgtcgttatc atggcggcgg ggtatcacgt catccgtgag
    16441 tgaccagcgg gtcctgatct gacgtttacg atcgcgatct ggctgaggtt cgtcccatat
    16501 caggcccagc cccagcatgt cccgtcgaag cccttcaatg gcctcttcca cacagggttc
    16561 cgggcagggg aggccgagac ggctggcttc attttctgcc cgggcaacgg ccaccacgcc
    16621 ggccccttcg atgaagcctt ttgtggtgtt tgcccggtga agggcataga agagcgtccg
    16681 gtcaacgccc ttgagccaca gccagttcgg aggcgtcagg cgcaaatcat gtgcgtaaag
    16741 ccagaccaga ccgctgcgaa cgtaacggtg ctgcctgagc cactgttgtg cgccttctgt
    16801 tttgatgacc cggataaagg cgtttctggc cagtgaatac accggcgtgc tgaaacgccc
    16861 ctggtcccgt ttacttttga gcctgcagga gagattgagg ttgttcatca gggctaccgc
    16921 tgctttccgg tcaccgagga agaactgcag gccaaagatg gcaaagagcg ctttttcatg
    16981 tggggccatg tctttccagc tggtcagtgg ttgccccagt tgcgccatga agcactggcg
    17041 tgtggacgca acatcgagct gcatgttgcg gataaggttg ttctgctcca caaagacttc
    17101 gggggttagt gcgacacggc gctcgggacg tttttgtccg tggaagagtc tgttgttgtc
    17161 tccatcagcc agaacggggg cgatcgccgg tgacagagct gaaaaaatat ggggcagtgt
    17221 gtggatattc agtggcctgc gggtaaaacg gggctgggcc ggatggtgaa accacatcca
    17281 cagaaagccg aatgtcatgg gaagcagaat caaccagagg atccccactg tccgggacat
    17341 aacatccagc cactggccga aagtgatggt tgccgcgttg ttagcagtga tggccagcag
    17401 gttgtaacgg ggggcggcaa agttgtgaaa gggcccccag tcaatcatgc cccacagtgt
    17461 gtgcaggatc aggcaggagg cgaagacgat ttcaggtaaa aagcgccaga tgataatcgc
    17521 aaagagcagg catatggcac cgacgatata ccatatagcc attggatctt cggcggcaga
    17581 cgtctgttgg taagacacgg ggattcctcc atcgataaat agataaattt cgggatgagg
    17641 ctgagtggaa ttaatgcgaa caatcgccgg taatgactgg gcagggctga attcagcagg
    17701 gcattttcac aggaacagca gaaggtggtg agagacaggc acaaaaaagc cgactggtga
    17761 ggccggcttt tttgcgatcc cagggagagt gggtggctct ggcccggagg aagtctcttt
    17821 tcaactaacg ttaccagaat atcgttatat tcacctgatc gcaatctgtt cgttacagtc
    17881 tattgttccg ggagtagcga tatcgcctga aacaagtata atcagcaagt tagccttgtc
    17941 tggagactta ccttcgcatc aatccatcgg gactatggat ctgtgctatt caaagacctg
    18001 gccggacaaa caggtggtgc tgcgtctggt gctctggccg gaggaagtct cttttcagct
    18061 aacaatgaaa gagctgttgt gacaggcaat gtaagagtaa acaacagtga tttcctccat
    18121 tgatggagaa
//
LOCUS       DAJTUN010000086        17785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTUN010000086 DAJTUN010000000
VERSION     DAJTUN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.086"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(19..807)
                     /gene="bioH"
                     /locus_tag="OO470_002696"
     CDS             complement(19..807)
                     /gene="bioH"
                     /locus_tag="OO470_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6251895.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            845..1528
                     /gene="gntX"
                     /locus_tag="OO470_002697"
     CDS             845..1528
                     /gene="gntX"
                     /locus_tag="OO470_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6251896.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            1587..2162
                     /gene="nfuA"
                     /locus_tag="OO470_002698"
     CDS             1587..2162
                     /gene="nfuA"
                     /locus_tag="OO470_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6251897.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            2523..3839
                     /gene="gntT"
                     /locus_tag="OO470_002699"
     CDS             2523..3839
                     /gene="gntT"
                     /locus_tag="OO470_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6251898.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(3884..5968)
                     /gene="malQ"
                     /locus_tag="OO470_002700"
     CDS             complement(3884..5968)
                     /gene="malQ"
                     /locus_tag="OO470_002700"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6251899.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(5978..8371)
                     /gene="malP"
                     /locus_tag="OO470_002701"
     CDS             complement(5978..8371)
                     /gene="malP"
                     /locus_tag="OO470_002701"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6251900.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8983..11688
                     /gene="malT"
                     /locus_tag="OO470_002702"
                     /pseudo
     CDS             8983..11688
                     /gene="malT"
                     /locus_tag="OO470_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(11731..12747)
                     /gene="rtcA"
                     /locus_tag="OO470_002703"
     CDS             complement(11731..12747)
                     /gene="rtcA"
                     /locus_tag="OO470_002703"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6251901.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(12751..13977)
                     /gene="rtcB"
                     /locus_tag="OO470_002704"
     CDS             complement(12751..13977)
                     /gene="rtcB"
                     /locus_tag="OO470_002704"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6251902.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14166..15764
                     /gene="rtcR"
                     /locus_tag="OO470_002705"
     CDS             14166..15764
                     /gene="rtcR"
                     /locus_tag="OO470_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6251903.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(15746..16504)
                     /locus_tag="OO470_002706"
     CDS             complement(15746..16504)
                     /locus_tag="OO470_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6251904.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(16521..17351)
                     /gene="glpG"
                     /locus_tag="OO470_002707"
     CDS             complement(16521..17351)
                     /gene="glpG"
                     /locus_tag="OO470_002707"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6251905.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(17396..17722)
                     /gene="glpE"
                     /locus_tag="OO470_002708"
     CDS             complement(17396..17722)
                     /gene="glpE"
                     /locus_tag="OO470_002708"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6251906.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
BASE COUNT         4119 a         4976 c         4464 g         4226 t
ORIGIN      
        1 ttaatcgacc cacaataatt agctctcact taccaccaac accctctgct tcaacgccac
       61 cagcaggtga caaaactcga ccggatgcga aataaacggc gcatgggccg ctttggcgaa
      121 gatatatgat tcgctgtgag gccaaagttt atccagcatc ggcaccactt tgcgcggcac
      181 cagaccgtcg agatagccat acaatcgcaa aaacggcatg gacacgtttt gcagcggctg
      241 acggagatcg accgttttca ggatttccag cccgccatta agcacgtcaa cctccggcat
      301 cggtaacgcc agaacggttt tcttcaacgc ccgcgcatcc tggcgcgccg tttcagtccc
      361 catggtttgt aacgccagga accgctccac tgtacgctga aaatcatcac tgagttgctg
      421 ctgaaatccc gccagcacgt ccggttttat ccccggccac tcgtcacgag cactaaaaca
      481 aggtgacgac gccacggtga ccagcgcctg aacacgctcg ggatgggtta acgcaatctg
      541 gcttgccacc agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgttg
      601 cagcacggct tcggccatat cagcaagtga cagcgcacca aatccccggc tacgcccgaa
      661 gccgggcagg tcaacaaggt gcagcgtaaa atgcgagcta agttcctcgt caatgcaacg
      721 ccacacttcg gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc
      781 tttggtctgc caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga
      841 acatatgcta acagtaccgg gattatgctg gctatgccga atgccactgg cgttaggtca
      901 ttgggggatt tgttcggtct gttcacgcgc cacccgcaca gataaaacgt tatgcccaca
      961 atgtggatta cccgccacac actcccatct tccctgcggt cgctgcctgc aaaaaccgcc
     1021 gccctggcaa agactggtca cggttgccga ctatgcgccg ccgttaagtc cgcttatcca
     1081 ccagcttaaa ttttcccggc gcagtgaaat cgccagcgcc ctgtcacgtc tgttactatt
     1141 ggaagtctta cacgctcgtc gcaccaccgg gttgcaattg ccggatcgca tcatcagcgt
     1201 tccgttatgg cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca
     1261 gccgttatca cgctggttgc actgccaatg ggatagcgaa gccgtcacac gtacacgggc
     1321 cactgcgacc cagcattttc tcagtgcccg gctgcgcaag cgcaacctga aaaatgcctt
     1381 tcgtcttgaa ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac
     1441 cggaagtacc gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca
     1501 ggtctggtgc ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta
     1561 aaatagtcaa ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact
     1621 ttgccaaact gctggcaaat caggaagaag ggacacaaat ccgcgtattt gtgattaacc
     1681 ctggcacgcc taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca
     1741 ccgacactgc cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgcaccat
     1801 acctggaaga tgcagagatc gactttgtta ccgaccagtt gggttctcag ttaacgctga
     1861 aagccccgaa cgccaaaatg cgtaaagtgg cagacgatgc accgctgatg gagcgcgtgg
     1921 agtatatgct gcagtcgcag atcaacccac agcttgctgg tcacggtggt cgcgtttcgc
     1981 tgatggaaat caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt
     2041 gttccatggt cgatgtgacg ctgaaagaag ggatcgagaa gcagctgctg aacgaattcc
     2101 cggagctgaa aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact
     2161 aagttatccc ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac
     2221 gttgaacatg tgccagacgt tggcacagca ttgctttggc aaatccctcc cttcgtttcc
     2281 ccgcgataat atgaccaacc tctcataatt taaatttacc ccgctctggt gattctcaaa
     2341 cgccaaatgt tacccgtatc attcacatgg gtaccaaaca tactcctgac atctgactac
     2401 aataattagt tttagtgggt atcagtcgtg gtgccgcaat atctctgttc ccgattggga
     2461 taattagagt ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa
     2521 tcatgccatt agtcattgtt gctatcggtg taatcttgtt gttgctcctg atgatccgct
     2581 tcaaaatgaa cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag
     2641 gaatgccgct ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta
     2701 gccttgccct gatcatgggt tttggcgcaa tgctgggcaa aatgctggca gactgcggtg
     2761 gcgcacaacg tatcgccacc acgctgattg ccaaatttgg taaaaagcac atccagtggg
     2821 cggtggtgtt aaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggcttcgtgc
     2881 tgatgctgcc gctggtgttt accatcgcgg cttccgcgaa tattccattg ctgtatgttg
     2941 gtgtaccaat ggcggctgca ctgtctgtga cccacggctt cctgccaccg catccgggcc
     3001 cgactgcgat tgccaccatt ttcaatgccg atatgggtaa aaccctgctg tacggtacta
     3061 ttctggcaat cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta
     3121 tcgataagcc aatcccggaa ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc
     3181 cgagctttgg cgtcagcgtc tggacctctc tggtgccggt tgtgctgatg gcgatgcgtg
     3241 cgattgccga aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg
     3301 gtgacccggt aatggcaacg ctgattgccg tgctgattgc gatgttcacc tttggtctga
     3361 accgtggtcg ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg
     3421 cgatgatgct gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg
     3481 gcgtggacaa atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg
     3541 cctggtcgat tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca
     3601 ctgcgggtgg tatcgcggca ccgctgattg caacgacggg tgttagccca gagctgatgg
     3661 ttattgcggt gggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc
     3721 tgttcaaaga gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg
     3781 aaaccattat ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat
     3841 aacactgact gccggatgca tcgcatccga catcaaccgc actctacttc ttcttcgctg
     3901 cagctctgcg ccgtctgtcc aaatccttca gcaacttgtt cacgccatca tcggcaaaca
     3961 tcgactcaag cgttgcggaa agcttgcgtc gccagttttt atactggtaa ctggtgccag
     4021 gaatattcac cggttcggcc atatccagcc agtcttccgg ctgtagtcct aacagagcac
     4081 tgttactgtc ggcaatgtag cgctgcaaac cacggttcag cgtcggcgtc atcgacatca
     4141 acgatgcctt atgcccggca cgttttggca gacaaccata tttatgcagt gcatccagca
     4201 gcccttgctt cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat
     4261 acagccccag ggttttgccc agcgttagat ccccgctctc ccagtaacca cgcagcgttg
     4321 gcaggtcatg tgtcgccgca accgccatcg actgctccgg atacgctttc ggtgcacgga
     4381 acgtcttctc gtggtcgttt tcgaaataga gcactttgta agagtacaca ccgctgctgc
     4441 gcagcttacc aacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc
     4501 gatgacgttt actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat
     4561 acgcgccctg atctgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca
     4621 catggtcaat tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg
     4681 gttcataggc acgcgcggtg atgatatgcg ggtccattgg cggtaatccc cagttctgcc
     4741 ccaacgggcc gaggatatcc ggcggcgcgc caaccgatgc tttcaggcaa tatagttcac
     4801 ggtcacacca ggtttccgcc ccaccttccg ctacgccaac cgccagatca cgatacaagc
     4861 caatcggcat ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcca
     4921 gccactgcaa ccagagataa aaatcgacgt catcacgatg ttcttcgcag aactgacgca
     4981 cttctggtga atccacgttc ttatacatct ctggccatgc aggccagccc cagcgcattt
     5041 cgtcctcttt cacttgctgg gcatgtagcg catcaaaggc agcctgccag aacaggctgt
     5101 cgccctgctc tgcaacaaac tggcgaaacg cggccatctg ctcatcatca cgttgcgcga
     5161 aacctttcca cgccattcgt aatgctgtca tttttagggc ggtaaccgtg gagtaatcga
     5221 cccagtcggc atcgcgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg
     5281 cctgagcctc ttcgctaaga tggaaatctt caacggcgtt aacgtcgata taaatcacat
     5341 tcagccaacg gcgagaagac gggctgtatg ggctggcgct ctccggattt gccggataga
     5401 gcgcatgaat cgggttcagg ccaatgaacg acccgccacg ttttgccaca tccaccagca
     5461 tcgctttgag atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa
     5521 gctgaacgca ggcaccccac agcttttgtt tattcagcaa agcctgcggt tcgtaacaac
     5581 gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg agtgttagcg
     5641 tgtgataacc ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gttacatggc
     5701 ctttgtactg cgttccttct tcggtggtca gctgccagct atattcgccg ctgccctcca
     5761 ccaccatcgg catttttttg ccgctggtat aaaccatgac attcgggact ggcgttaccg
     5821 ccactttcgt ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc
     5881 taatcgactg cggtttaccg tgggcattga tgtaattggg gctaatcccc gccgccagcg
     5941 cggcattatc cagacgtttg ctttccatcg agcttcctta gcgttttgcc tgccagatac
     6001 gagcctgata atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga
     6061 ggatcgccgc gcgagtccag gcctcctggt cgcggtacag cacatccacc tgcttttgtg
     6121 cctcaacata ggctgcgaaa tccgccatca ccagatacgg atcgccgccc tgtttgccga
     6181 tactgtgcag catctggtcg aaggcatgct tatcgccgtc gctgtattta ccgctttcca
     6241 gctctttcaa tactgcgttc agcaccttat ctttcttccg ccatttcacc gggtcgtagc
     6301 ctttggccag aatagccttc acttgttcca cggtatgacc aaaaataaag atattttctt
     6361 caccgacttt ctcggcgatt tcaacgttcg caccatccag cgtaccgaca gtaagcgcac
     6421 cattgagcgc cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt
     6481 gttcggagat atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa taatccggca
     6541 ggaacaccac cttcaactta tcgccaacca gtggatcgtt gttgatcacg tcagccactt
     6601 tgttgatcgc aaagataata ttcttcgcca ggtagtagcc cggtgccgct ttcgcgccga
     6661 agaggaagac gcgcggtacg cgatcagcct gcaggttttc acgaatttct ttgtacaacg
     6721 ccagaatatg cagcagattc aggtgctggc gtttgtactc atgcagacgt ttgatctgaa
     6781 tatcgaaaat cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactccgcca
     6841 gacagacttt attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag
     6901 cgaatttttc cagattgatc agctgatcga gatcgttagc ccactctttt tgcagtgatt
     6961 tatccaacag agccgccagt gcagggttgc actgtttgat ccagcgacgt ggggtaatac
     7021 cgttggtaac gttatggaat ttgttcggcc atagctggtg atattccggg aacagatctt
     7081 tcaccaccag atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac
     7141 acaggttcgc catatgcact tgtttatcgt gcaccaccgc cagtttggcc cacacttttt
     7201 catcgcccgg ccaggttttc tccaccagcg ttttaaagcg agtattaatt tcgttaataa
     7261 tctgcatatg gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt
     7321 ctggcatcag ggtatggttg gtgtaagcga aagttttgct ggtgatggcc caggcgtcat
     7381 cccagctcat ctggtgctca tcggtcagca cgcgcagcag ttctggaatc gcgatagttg
     7441 gatgggtatc gttcagctga ataacttcgt aatccgccag ttcgtgcagt ttacgccccg
     7501 ccagatgatg gcgacgcaaa atatccgcta ccgaacaggc acactggaag tattgctgca
     7561 tcaggcgcag ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt
     7621 tttccgcatt gatgccttgc tgttcggcgc gcaggaaatc accgtcgtta aatttagtta
     7681 gatcaaacgg atgcgcatgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac
     7741 gatagccgac aacggggaga tcccacgctt gaccggtaat ggtaaactcc ggctcccagc
     7801 gtccgtcttt catcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc
     7861 ggaaccacgg gtagttactg cgatgccagt catccggcgc ttcaacctgt ttgccatcga
     7921 caaaagactg gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac
     7981 cgacagttgc cattgagtcg aggaagcacg ccgccagacg tcccagacca ccgttaccca
     8041 gcgccgggtc gatctcttct tccagcaggt cagtcaggtt gatgtcataa gccttcaacg
     8101 aatcctgtac atcctgatac cagccgagat tcaacaggtt gttgcccgtc agacgaccaa
     8161 tcaaaaactc cattgagatg tagttaacat gtcgctgatt cgccaccggt ttggcgaatg
     8221 gctgagcacg cagcatttcg gccagtgctt cactcactgc cagccaccac tggcgaggag
     8281 tcatttcagc cgcagaattt aagccataac gctgccactg acgtgaaagc gcttcctgaa
     8341 attgcttatc gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac
     8401 acaacgttat cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta
     8461 ggcagggagg agttgcgggg atgagcaagg aaatgtgatc tcgaccactt aaagctagtg
     8521 caaaccacag gattagcagc aaatcaatgc catacagcgc agaaaatctg tatctaagtg
     8581 caaaaaatgg ccgttgcgta ttttcaaaaa gcggaaggta actctataaa ttaagtaaag
     8641 gagtgaaaca gtttcacaag taaaatatcc agtgtgctcc atctcattct taatagattt
     8701 attaagatca tctttttaga tggcactttc atcaggaatg aagaagaaac ccttgcttaa
     8761 atgaatctga tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg
     8821 agttgttaat aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt
     8881 catcgcttgc attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa
     8941 aaaaataata tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa
     9001 ctaagtcgtc cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt
     9061 tccggcgcga acaacttccg gctggcgctg atcacgagtc ctgcgggcta cggaaagacc
     9121 accctcattt cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggatgaa
     9181 ggtgataacc agcaagagcg tttcgccagc tatctcattg ccgccgtgca gcaggcaacc
     9241 aacggtcact gtgcgatatg tgagacgatg gagcaaaaac ggcaatatgc cagcctgacg
     9301 tcactcttcg cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc
     9361 atcgatgact atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt
     9421 cgccatcaac cagaaaatct caccctggtg gtgttgtcac gcaaccttcc gcaactgggc
     9481 attgccaatc tgcgtgttcg tgaccaactg ctggaaattg gcagtcagca actggcattt
     9541 acccatcagg aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca
     9601 gaaagcagtc ggatttgcga tgacgtttcc ggttgggcga cggcactata gctaatcgcc
     9661 ctctccgccc ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc
     9721 aatgccagcc atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca
     9781 acgcgccatt ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc cctcatcacc
     9841 cgtgtgaccg gcgaagaaaa cgggcaaatg cgtctcgaag agattgagcg tcaggggctg
     9901 tttttacagc ggatggatga taccggcgag tggttctgct atcacccgct gtttggcaac
     9961 ttcctgcgcc agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc
    10021 gccgcagaaa gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg
    10081 gcaggcgatg cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac
    10141 catagcgaac tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg
    10201 gaaaatccgc agttagtgtt attgcaggcg tggctaatgc aaagccaaca tcgctatggc
    10261 gaagttaaca ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc
    10321 atgcatgcag aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatcaggat
    10381 gaagcggaac ggctggcaaa actggcactg gaagagctgc cgccgggctg gttctatagc
    10441 cgcattgtgg caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc
    10501 tcactggcgc tagtgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac
    10561 gccttatgga gtttaattca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc
    10621 gcgtgggaaa cgcaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg
    10681 ccaatgcatg agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg
    10741 gatgaagccg aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag
    10801 caacagcttc agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat
    10861 aacgcccgta gccagttgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc
    10921 gactggatct ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa
    10981 gccgccgctg ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc
    11041 ctgcaaggtc aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct
    11101 gcggaaattg ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat
    11161 ctcaaccgta acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac
    11221 gcccagcgcg tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagtcat
    11281 tttgtcatcg aaggcgaagc gatggcgcaa caactgcgcc agctgattca acttaatacg
    11341 ctgccggaac tggaacagca tcgcgcgcag cgtattctgc gagaaatcaa tcaacatcat
    11401 cggcataaat tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa
    11461 gtgccagaac taatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg
    11521 atctactccg gttacagcaa tgaacaaatt gcgggcgaac tggaagtggc ggcaaccacc
    11581 atcaagacgc atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc
    11641 gtgcagcacg cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttagccggat
    11701 aatgcattgg atccggttta catctttaca tcattcaata ctcacccgcg ttacgccatc
    11761 tgtttctatc aaaccaaacc gcaccggcaa gaaacgctcc accaccgcga tattggtcag
    11821 cagatggcat gaaggatggg cgaccgtaaa ttctcccgcg cccgccagcg ccatcggcag
    11881 taccagttgg tcggcgagat attcccccac cgccgctggg cttgccaggt agcgtttcac
    11941 ctctttcact aactgcgcgg ctaccacctc ggcactgacg cgcttttcac cgacgacgaa
    12001 aaagcgttca gtgatatttt cactttcgac ttccagcgag acggtattac ccggcccctg
    12061 gtcgcgcggc aggttatgaa tattctgttc atgcagggaa aaaatccccg ccagtgtagc
    12121 gatttcacgc tcagcaacat ggcgcggcac accagctaat agaacttctc cacgcatctg
    12181 cacaatgttc ccgcgctcgc caagttgcaa ggtgctaaac gatgccacgg gcgagacttc
    12241 cgttgccacc acaccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg
    12301 atgaattcct attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg
    12361 cgccgacggg ttatcggtgc cgccgctcac ttcaacacgc gaaggtccat cggcaaacca
    12421 cagcgcgggc agcaccgttt gcagcaccag cgtacaactt ccggcgctac cgatagcaaa
    12481 gcggtaatcg ccgccgcgca cggtgccagg ccggaagacc agacgctgcg atcccagctc
    12541 cgcgccttcc accgttgccc tacaaatttc cgcagccgct tttaccgcgg tcagatgctg
    12601 gcgcagcagc cccggtttcg cccgtccggc tcgaatgcca gtgatggtaa atggctggcc
    12661 ggttatcatc gacaggctca gcgccgagcg caggatctgc ccgccgcctt cgccctgtgc
    12721 accatccagc gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag
    12781 ggtgtagata acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata
    12841 cgccatcggg atttcgtcga tcacttcggc atctttacgg cattccacat gcgcggtggc
    12901 acgaatttgg tcttccacgc tgaacagttt tttcgcttta gttcggctca ttacccgccc
    12961 ggcaccgtgg ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct
    13021 ttttgctccc atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcacc
    13081 tttacgcgtc acgtagatct cttcaccaaa gagctgttct ttttgcacat agttgtggtg
    13141 acagttgatc tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct
    13201 ctgcaacgcc gttaccacat tttccatcat cgcatcgcgg ttaaggctgg cgaaaagctg
    13261 cgcccaggcc acggctctta ggtaatcatc aaagtattcc gtaccttcca taaagtacgc
    13321 cagatcacgc gacggcaacg tctcaagttg gtcctgcatc tctttttgcg ccagatcgat
    13381 aaagtaagtc ccgatggcgt taccaattcc gcgtgaaccg gagtgcagca taatccacac
    13441 ctgctccgac tcatcaaggc agatttcaat aaagtggtta cccgttccca gcgttcccag
    13501 gtgtttatag ttgttagtgt taaggaaacg cggatatttt tgcgttaacc actgataacc
    13561 ggcctccagc tcagcccatt tcgcatcgac gttaaaaggc gggttttccc atgcgccttt
    13621 atcacgttta caacgcccgg tagtacgccc gtgtggtacg gccgtttcaa tcgcttgacg
    13681 cagctccgcc aggttttcag gcaggtctgc cgccgttaac gcggtacgca gcgcgttcat
    13741 tccacagcca atatccacgc ccaccgccgc cggaataatc gcccctttgg tcgggatcac
    13801 gctaccaatg gtggaacctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa
    13861 aataaacggc atcttcgccg tattaataag ttgctgacgc gcatcggctt ctaccggcac
    13921 gcctttggtc cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt
    13981 gtttttctct tttcgttgtt tgcggtcctg ataattgcaa ccgtcgtgcc agaaaattaa
    14041 ataagcagat taatttttta atttattgtt tctaaaaaga ttatatcttt atgtccgtaa
    14101 acggagattt cccgcaaagc cgatttaccg ataatgaaat atcgcctttt ataaggatat
    14161 ctaagatgcg taaaacagtg gcttttggct ttgtcggtac cgtgctggat tatgccggac
    14221 gcggcagtca gcgctggtca aaatggcgtc cgtcactctg tatataccag caagaatcgt
    14281 tggtcatcga tcgactggaa ttattgcacg atgcccgctc gcgctcgcta tttgaaacgc
    14341 ttaaacgcga tatcgccagc gtttcgccag aaacagaagt ggtgggcgtt gagattgaac
    14401 tgcataaccc gtgggatttc gaagaggttt acgcctgcct gcatgatttc gcccgtggtt
    14461 acgagtttca gccagaaaaa gaagactatt taattcacat caccaccggc acccacgtcg
    14521 cgcagatttg ctggtttctg ctggcggaag cgcgttatct acccgcccgg ttgatacaat
    14581 cttcaactcc gcgcaaaaaa gaacagccgc gcggcccagg tgaagtgacg attatcgatc
    14641 tcgatttaag ccgttataac gccatcgcca gccgctttgc cgaggaacgg cagcaaacgc
    14701 tcgatttcct caaatccggt attgccacgc gtaaccccca cttcaaccgc atgattgagc
    14761 agatcgaaaa agtggcgatt aaatcccgcg cgccgattct gcttaacggc ccgaccggcg
    14821 caggtaagtc atttctggcg cgacgcatct tcgagttaaa acaggcacgg catcagttta
    14881 gcggcgcgtt tgtggaagtg aactgcgcca ccctgcgcgg cgataccgcg atgtcgacgc
    14941 tgtttggtca tgtgaaaggc gcgtttaccg gggcgcggga atctcgtgaa ggattattac
    15001 gcagcgccaa cggcggaatg ttgtttcttg atgagattgg cgaactgggc gcagacgaac
    15061 aggcaatgct gctgaaagct attgaagaga aaacctttta cccgtttggc agcgatcgcc
    15121 aggtgagcag tgattttcag cttatcgccg gaacggtgcg cgatttgcgc cagctggttg
    15181 ccgaaggcaa attccgcgaa gatctgtacg cgcggatcaa tctctggacc ttcaccctgc
    15241 cgggtctacg ccagcgccag gaagatattg aaccgaacct ggattatgaa gtggagcgcc
    15301 acgccacact cactggtgac agcgtgcgtt ttaacaccga agcgcggcgt gcctggctgg
    15361 cctttgcaac atctccccag gcaacatggc gcggtaactt tcgcgaactt tctgccagcg
    15421 tcacgcggat ggccaccttt gccactagcg gacgcatcac tctggacgtg gtggaagatg
    15481 agataaaccg tctgcgctat aactggcagg agagccgccc ctccgcgctt acggcgttgc
    15541 tgggcgcaga ggcagaaaac atcgatctct tcgaccgtat gcaactggaa cacgttatcg
    15601 ctatctgccg ccaggcaaag tcgctttctg ctgccggacg acagcttttt gatgtttcgc
    15661 gccagggcaa agccagcgtc aatgacgcgg atcggctacg caaatacctg gcgcgttttg
    15721 gtctgacgtg ggaagccgtg caggatcagc acagctccag ttgaatatgg tggtccgtca
    15781 gcacctgcat cacgcttact ggcggcgggg cgtcggtgta gacggcatca accatgctga
    15841 tgctccccat attgaccatc gcattacggc caaattttga atggtcaacc accagcataa
    15901 cgtggcgcga gttctcaata atggcgcgct tggtacgaac ttcgtggtaa tcgaactcca
    15961 gcagcgagcc gtcgctgtcg atgccgctga tccccagaat gccgaaatca aggcggaact
    16021 gggagataaa atcgagcgtc gcttcgccaa tgatcccgcc atcgcggcta cgtaattcgc
    16081 caccggcgag aataatgcgg aaatcttctt ttaccatcaa cgtgttagca acgttgagat
    16141 tgttggtgac aatgcgcaaa ttgctgtgat tgagcagcgc gtgcgccacc gcttccggcg
    16201 tggtgccgat atcgataaac agcgtcgagc cattggggat ttgctccgcc actttgcggg
    16261 cgatgcgctc tttttcttcg gtctgggtgg ctttacgatc gtgccacggc gtgttaaccg
    16321 aactggaagg cagcgccgcg ccgccatgat ggcgcaggat caggttttgc tctgccagct
    16381 cgttgaggtc gcggcgaata gtctgcgggc tgacggagaa gtgctccacc agttcctcgg
    16441 tactgacata accctgctgt ttaaccagtt cgataatacc gttgtgacgt tgtgtttgtt
    16501 tcatttataa atccctggaa ttattttcgt tttcgcgcat tgagcgaatc aacaaaagcc
    16561 atcgctaaac ccacggctaa tccggcgatg tgtgctccgt tcgccatcga catcccaaac
    16621 aaatcaaacc atccggcgac aatccagatc agcgcaaaga taattaaccc acgttgcagg
    16681 taaatgccac tttgcggatc gcgttcgcca cgtagccaga cgtagcccat cagcgcatac
    16741 accacgccag aaagcccgcc aaaccacggc ccgctgaatt tttgctgcat atagccgctt
    16801 aacagggcgc tgataagcgt aatgacaatt agcttaccgc taccgaggcg tttttccacc
    16861 gcgccgccga gataccacca ccaaagcagg ttaaagagga tatgcatcag cgagaagtgc
    16921 attaacgcgt gggtgaagta acgccagaac tcaaatttca gtgttggatc gaatggccag
    16981 gccagccata acatcacttc ctgatcgccg agaatttgca tggcaataaa caccaccacg
    17041 caggcgatca tcatcaccca ggtcaccgga cctgcgcgtt cacgcaaggc agcaaagaaa
    17101 ggataacggc gataatgcag gccactgccg gtatggcctg ccagccagct ggccgccaga
    17161 taacgcggat ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgcac gcgctcggcc
    17221 tgggactcat ccgccagcca gacatcgctt tggttatgtt gttgaatcgt gaggataaca
    17281 ccctgcgtcg ccatgtaatc aacaaacgcc tgcgccacgc gggggttagc aaaagaggta
    17341 atcatcaaca tcgttgctgt cgcttattcc acacaaaagg ggacagtata aagcgttacg
    17401 cgccgtacgc cacctctgcg ggaaactgtc gctgccaggc ttcaaagccg ccgtcaatgc
    17461 tatagaccac atcgtagccc tgttgcagca gatactgcgc cgcccctttg ctgctattgc
    17521 cgtgataaca catcaccatc accggagtgt caaagtcgtt atcacgcata aacgcgccca
    17581 gcgtgtcgtt ggttaaatgg aaagcctgca ccgcatgccc catagcgaaa ctctgtggat
    17641 cgcgaatatc gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg tcggcaacgt
    17701 taatacattc gaactgatcc atgcgtctct ctttctttac aaacaagtgg gcaaatttac
    17761 cgcacagttt acgtcgaagc ggcag
//
LOCUS       DAJTUN010000087        17378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTUN010000087 DAJTUN010000000
VERSION     DAJTUN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.087"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            132..575
                     /locus_tag="OO470_002709"
     CDS             132..575
                     /locus_tag="OO470_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6251907.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1629
                     /gene="ybhA"
                     /locus_tag="OO470_002710"
     CDS             811..1629
                     /gene="ybhA"
                     /locus_tag="OO470_002710"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6251908.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLLSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1630..2688)
                     /gene="modC"
                     /locus_tag="OO470_002711"
     CDS             complement(1630..2688)
                     /gene="modC"
                     /locus_tag="OO470_002711"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6251909.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2691..3380)
                     /gene="modB"
                     /locus_tag="OO470_002712"
     CDS             complement(2691..3380)
                     /gene="modB"
                     /locus_tag="OO470_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6251910.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3380..4153)
                     /gene="modA"
                     /locus_tag="OO470_002713"
     CDS             complement(3380..4153)
                     /gene="modA"
                     /locus_tag="OO470_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6251911.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="OO470_002714"
     CDS             complement(4320..4469)
                     /gene="acrZ"
                     /locus_tag="OO470_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6251912.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4598..5386
                     /gene="modE"
                     /locus_tag="OO470_002715"
     CDS             4598..5386
                     /gene="modE"
                     /locus_tag="OO470_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6251913.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5454..6926
                     /gene="modF"
                     /locus_tag="OO470_002716"
     CDS             5454..6926
                     /gene="modF"
                     /locus_tag="OO470_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6251914.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7187..8203
                     /gene="galE"
                     /locus_tag="OO470_002717"
     CDS             7187..8203
                     /gene="galE"
                     /locus_tag="OO470_002717"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6251915.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8213..9259
                     /gene="galT"
                     /locus_tag="OO470_002718"
     CDS             8213..9259
                     /gene="galT"
                     /locus_tag="OO470_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6251916.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9263..10411
                     /gene="galK"
                     /locus_tag="OO470_002719"
     CDS             9263..10411
                     /gene="galK"
                     /locus_tag="OO470_002719"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6251917.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10405..11445
                     /gene="galM"
                     /locus_tag="OO470_002720"
     CDS             10405..11445
                     /gene="galM"
                     /locus_tag="OO470_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6251918.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11648..12400
                     /gene="gpmA"
                     /locus_tag="OO470_002721"
     CDS             11648..12400
                     /gene="gpmA"
                     /locus_tag="OO470_002721"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6251919.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="OO470_002722"
     CDS             complement(12567..13619)
                     /gene="aroG"
                     /locus_tag="OO470_002722"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6251920.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13935..14315
                     /locus_tag="OO470_002723"
     CDS             13935..14315
                     /locus_tag="OO470_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6251921.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14429..15370
                     /gene="zitB"
                     /locus_tag="OO470_002724"
     CDS             14429..15370
                     /gene="zitB"
                     /locus_tag="OO470_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6251922.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="OO470_002725"
     CDS             complement(15367..16086)
                     /gene="pnuC"
                     /locus_tag="OO470_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6251923.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="OO470_002726"
     CDS             complement(16124..17167)
                     /gene="nadA"
                     /locus_tag="OO470_002726"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6251924.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4430 a         4539 c         4316 g         4093 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg caaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaattgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 gccacttgta attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttctat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat
     1021 ttgtatgatt atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc
     1081 ctgcaactca ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgat
     1141 gcaatggtct atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc
     1201 ctgccgccgg aacagcgtcc gactttcaca caagtcgctt ctctggctga aacgacgcaa
     1261 caagttaacg ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat
     1321 tttggtaagc atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag
     1381 gttgatattg cacacggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg
     1441 caaggttggt cgatggaaaa cgtcgtggca ttcggcgata actttaatga tatcagtatg
     1501 ctggaagccg ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc
     1561 gccaacattg tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac
     1621 ctgatttaat caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg
     1681 tttgatcgcc agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc
     1741 gacttccagc tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg
     1801 caatacgtta cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc
     1861 ctgaatgcgg atacgtagcg cagcttgcag cggttcgccc agcttattga cccacaaatg
     1921 ctgatcgccc agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gcaccgtcac
     1981 tttcagaatg ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca
     2041 cacttcctcc agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc
     2101 tgccagatgg aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc
     2161 ccgtgtcagc cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc
     2221 cagcggttca tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac
     2281 gcgctgtttt tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc
     2341 taaaagcgcc accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag
     2401 attgccacgc actttgtaat gcgggaacaa ccgcgcatcc tgaaaaacat agccaacacg
     2461 acgcttttcc ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag
     2521 gacaatccgc cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt
     2581 tccggcaccg gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat
     2641 ggtcaggcaa tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc
     2701 gcccgttcac ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc
     2761 gcaatagaaa taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg
     2821 gtatacatgg cagaaggaat ggttcgcgtt tcgctaggaa tgttcgacac aaaggtgatg
     2881 gttgcaccaa actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc
     2941 ggtaaggtca gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt
     3001 cttgcggcct gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc
     3061 atcagcggaa acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag
     3121 gtaataccaa accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact
     3181 aataataagt aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg
     3241 agcagagctt tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac
     3301 ggcaggctaa acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat
     3361 tctggatcgg tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg
     3421 cctgcggtcc cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca
     3481 caacagcaac cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt
     3541 ttaccccttt gctggcaact gcgtcagaac cgtagacaat gcccagaggc gcttcgttac
     3601 gttcgaccag cgccagcgcc ccacgaacat cttccgctgg ggccagtttc ggagagagcg
     3661 tatcccatgc gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt
     3721 ccggatcgcc aaccgccagg cgaccgccat tcagcagcga agtccagtta gttttgctgt
     3781 cgatggtgaa atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga
     3841 gcagtgtctg acgcgtagca gtatcgatcg cttttttatc aaccgcataa tccatccatt
     3901 tctgatcggc agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag
     3961 tagatgacga agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag
     4021 caatgtcctg cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat
     4081 ctgccagtgc attgccagca actgcgaaag agagtgccgc cccggcaaac aagttcaacc
     4141 atttacgagc cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta
     4201 actcggaatt ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg
     4261 gcaggaaaag caggaagttg agaaaaagaa aatgcccgac taagcgggca ctcagggaat
     4321 caatgatttt gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca
     4381 ccaagaccgt aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg
     4441 aataccagac tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt
     4501 ctaaccgcta agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg
     4561 ttttccgtca caataagact tttgccagga cattgttatg caggccgaaa tccttctcac
     4621 ccttaagctc caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat
     4681 tgcgctttcc ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc
     4741 ctgggatgcc attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac
     4801 aggcggtaaa ggtggcggag gcgcagtact gacccgctat ggtcagcgac tgattcagct
     4861 ctatgactta ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc
     4921 cctgccgctg aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg
     4981 taaccagtgg ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga
     5041 tgtcttactg gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc
     5101 acgtctgggg ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg
     5161 cattactcag gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag
     5221 tcatattgag cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac
     5281 actgtgcgcc acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac
     5341 ggcctacttt aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt
     5401 tgttatgaaa cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat
     5461 cgttgcaaat tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc
     5521 agctaacgtt aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agtggaaagt
     5581 cggcgctggc ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc
     5641 agttttccca catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat
     5701 ggcaacggaa taacaccgat atgctcggcc ctagcgaaga ttacaccgga cgcactacgg
     5761 ctgagattat tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt
     5821 tcggtattac cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa
     5881 aaaccctgct gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt
     5941 tcgatggcct ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc
     6001 agtccggtat tactttggta ctggtgctca atcgcttcga taagatcccg gaatttgtcc
     6061 agtttgctgg cgtgctggcg gattgtacgt tagcggaaac tggcgctaaa gaggaactgc
     6121 tccagcaagc actcgtcgcg caacttgcgc atagtgaaca gcttgaaggt gtgcaactgc
     6181 cggagccgga tgaaccttca gcacgtcacg ccttacccgc caacgaatcg cgcattgtgc
     6241 tgaacaatgg cgtggtttct tataatgatc gccccattct taataacctt agctggcagg
     6301 tgaatccagg cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat
     6361 taagcctgat tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggcc
     6421 gacgtcgcgg cagcagcgaa accatctggg acatcaaaaa gcatatcggt tacgtcagca
     6481 gtagtttgca tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct
     6541 attttgattc gattggcatt tatcaggtcg tttcggatcg ccagcaaaaa ctggtgcagc
     6601 agtggctgga tattctcggc attgataaac acacggctga cgctccgttc catagtcttt
     6661 cctggggaca gcagcgtctg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc
     6721 ttattctcga tgaaccacta caggggcttg atccgctcaa tcgccaactt atccgccgtt
     6781 ttgttgatgt gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg
     6841 aagatgcgcc tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc
     6901 gctatgtgct gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcggcctaa
     6961 gcgcactctc cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat
     7021 aaaaagcgca atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac
     7081 ggctaaattc ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt
     7141 tgttatgcta tggttatttc ataccacaag cctaatggag cgaattatga gagttctggt
     7201 taccggtggt agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca
     7261 tgatgtcatc attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga
     7321 gcgtttaggc ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat
     7381 gaccgagatc ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc
     7441 cgtcgccgaa tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct
     7501 gcgcctgatt agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc
     7561 caccgtttat ggcgatcagc cccaaattcc atacgttgaa agcttcccga ccggcacacc
     7621 gcaaagccct tatggcaaaa gcaaattgat ggtggaacag atcctcaccg acctgcaaaa
     7681 agcccagccg gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc
     7741 gtcgggcgat atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc
     7801 ccaagttgct gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga
     7861 agatggtact ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt
     7921 ggcgatggaa aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt
     7981 aggcagcagc gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa
     8041 ctatcatttt gcaccgcgtc gcgaaggcga ccttccggcc tactgggcgg acgccagcaa
     8101 agccgaccgt gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac
     8161 ctggcactgg cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca
     8221 atttaatctc gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct
     8281 ggtttcaccg caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca
     8341 ggtgttacct gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg
     8401 cgataaaaac cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat
     8461 gtctgacacg ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg
     8521 cggtaccagc cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag
     8581 cgttgcggca ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa
     8641 aacgtacccg tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc
     8701 gcatccgcac ggacagattt gggcaaatag cttcctgcct aacgaagctg agcgcgaaga
     8761 ccgcctgcaa aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca
     8821 gcgcgagctg gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt
     8881 gccttactgg gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttgcg
     8941 gatcaccgat ttgaccgacg cccagcgcag cgatttggcg ctggcgttga aaaagctgac
     9001 cagtcgttat gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc
     9061 accatttaat ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct
     9121 gctgcgctcc gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca
     9181 gcgagacctg accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt
     9241 tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa
     9301 cgcatttggc taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg
     9361 tgaacacacc gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt
     9421 gatcagctgt gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa
     9481 tcagctcgac gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc
     9541 gaactacgtt cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt
     9601 ggacatggtg atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact
     9661 ggaagtcgcg gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca
     9721 aatcgcgctt aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat
     9781 ggatcagcta atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgccgctc
     9841 actggggact aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa
     9901 cttcaaacgt accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg
     9961 tgcgcgtttc ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaacgctgt
    10021 tgcacatgag ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc
    10081 ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt
    10141 gatggccgag tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga
    10201 cactctggta gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg
    10261 cggcggattt ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca
    10321 gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg
    10381 taaaccatca caaggagcag gacagtgctg aacgaaactc ccgcactggc acccgatggt
    10441 cagccgtacc gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac
    10501 tggggtgcga ctttactttc cgcccgtatt ccgctttccg atggcagcgt ccgcgaggcg
    10561 ctgctcggct gtgccagtcc ggaatgctat caggatcagg ctgcgtttct gggggcctct
    10621 attggtcgtt atgccaaccg catcgccaat agtcgctata cctttgacgg tgaaaccgtg
    10681 acgctttcgc caagccaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa
    10741 cgtcgctggc agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat
    10801 gatggcgatc agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac
    10861 gataaccgta tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg
    10921 actaatcacg tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg
    10981 cagattctgg cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg
    11041 aaatctgtcg ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt
    11101 cttgccgacg acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc
    11161 aaaggcgatg gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg
    11221 aaggtctaca ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca
    11281 ccgtcgcggg gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgagttt
    11341 ctgccggaca gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc
    11401 gaagagtatt ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc
    11461 atgagggctt tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc
    11521 aatattcaca acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg
    11581 ctgttgcttc gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag
    11641 taagcatatg gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa
    11701 agaaaaccgt ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc
    11761 aaaagcagca ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc
    11821 tgtgctgaaa cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg
    11881 gctgcccgtt gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct
    11941 gaacaaagcg gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg
    12001 ttttgcagtg actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc
    12061 gcgttacgcg aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat
    12121 tgaccgcgtg atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg
    12181 cgtaatcatc gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat
    12241 gagcgaagaa gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt
    12301 cgacgagaat ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc
    12361 gaaagcagca gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat
    12421 gagaaagccg actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca
    12481 ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact
    12541 ctgacggcgc atccgacaat taaaccttac ccgcgacgcg cttttactgc attcaccagt
    12601 tgacgtaaca gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg
    12661 taggccagcg gctccccgct atcgaggctc tgattgcctt ccaccagatg gctttccacc
    12721 atcacgccaa taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca
    12781 tccatctgct ttttgaattg tttggacgaa ttagcatggc tgaaatctat catcacctga
    12841 gctggcagac ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag
    12901 ttaggctctt taccgccgcg cagaatgata tggcaatcgc cgttaccact ggtattcaca
    12961 atcgccgaat gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta
    13021 atggcatcga tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa
    13081 gaaagaccag acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg
    13141 ccccagctca tcaggtcagc gagatactga ggagtgatca tatcgagaaa ctcacccgcc
    13201 gctggcagac cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg
    13261 ttgatctgga agctgttatc catatgcgga tcgttaatca accctttcca gcccaccgtg
    13321 gtacgcggct tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca
    13381 cgcagcgcca gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac
    13441 gggccaatca ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt
    13501 cgggcatggg caaccgtatt cgcggcattt tcagttgcgg ggaatttttc cagcaatgcg
    13561 acaggaggaa gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg
    13621 tctgttccag tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc
    13681 gtcagaatgt gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa
    13741 aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt
    13801 gtttgttaat tatttcaaga ttctctgctg cgtttcataa actggctgaa atcttaaacc
    13861 aatgccttat attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat
    13921 aacaggagca tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa
    13981 gccttgccag cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg
    14041 caaacgccgc agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa
    14101 ataacgtcga caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg
    14161 gttcttcaat gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca
    14221 aagatggtcg atgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg
    14281 atgtcgatac caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga
    14341 gagcggagtc tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta
    14401 agtgagaata attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca
    14461 cctgccagaa gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt
    14521 tatgctggta gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc
    14581 gggtcatatg ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc
    14641 acgtcgtcct cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc
    14701 gtttgtgaat gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga
    14761 acgcttccgc acgccgcgtc cggtcgaggg aggcatgatg atggcaattg ccgtggctgg
    14821 gttgctggca aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct
    14881 caacgtacga gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat
    14941 tattgccgcg ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat
    15001 actggtgtcg cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga
    15061 attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg
    15121 ggaaatcccg gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc
    15181 ggtgatgacg ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca
    15241 gatccaacac tacctgatgg atcactatca gattgagcac accaccattc agatggaata
    15301 tcaaccttgt cataggccag actgccacct gaatgaaggg gtgtctggcc attcacatca
    15361 tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg
    15421 gctgccgttg agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc
    15481 ctgaagtgca aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc
    15541 cacatactta cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc
    15601 ccagaacggg aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg
    15661 catgatcatg actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat
    15721 cagaccaatc gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca
    15781 gcgaattttc aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta
    15841 aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa
    15901 gaaaataatg ccaaacaagg taacgttgat caggccaaag aagtagttgc tgatcttctc
    15961 cagactggca aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga
    16021 gagatcataa ccgcccgccc ctattggtat atgtaccagg atattctgca cactaaaaaa
    16081 atccatcttt cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag
    16141 ccgcaaaatc cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctctcatcga
    16201 catgaacctc gtgattactt ccttcctgtt ctaatgcctc tgcgatagcc tgaaggtcat
    16261 tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg
    16321 gtgcttccag taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat
    16381 cggttgccac aataagcctc tgatgaggca atgcttttgc agcagcgatc agttgactgg
    16441 tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta
    16501 tggcagcatc cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat
    16561 gcacaatgca ggcaccctgc cagcataaaa tgtctccacc cgtctgtttt tgcacgtaac
    16621 gccccagatg tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa
    16681 taagttcgac ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag
    16741 aagtgttggc gtagacgacg acagtacgat cgggatgggc atcgtaaaat gcgttaaatt
    16801 cttcaacagg gcagccgaga tccagtgaac actcaacctg aagtgtcggc atcagaattg
    16861 ttttttccgg actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca
    16921 aagtagaagc gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc
    16981 caccggtttc ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca
    17041 ccgcattacg ttcttttagc aaacgtttta tcttctcgcg gtaatacgct ttttcatcaa
    17101 tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta
    17161 cgctcatctt accatctcgt tttacaggct taacgttaaa accgacatta gcgtaacatt
    17221 cgctgttatc ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa
    17281 gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg
    17341 atgtcatcag gtttacaaag tgaaattgag gatgcctg
//
LOCUS       DAJTUN010000088        17044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTUN010000088 DAJTUN010000000
VERSION     DAJTUN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.088"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..216)
                     /locus_tag="OO470_002727"
     CDS             complement(<1..216)
                     /locus_tag="OO470_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6251925.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
     gene            526..2298
                     /gene="aspS"
                     /locus_tag="OO470_002728"
     CDS             526..2298
                     /gene="aspS"
                     /locus_tag="OO470_002728"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6251926.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2416..2868
                     /gene="nudB"
                     /locus_tag="OO470_002729"
     CDS             2416..2868
                     /gene="nudB"
                     /locus_tag="OO470_002729"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6251927.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2897..3637
                     /locus_tag="OO470_002730"
     CDS             2897..3637
                     /locus_tag="OO470_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6251928.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3672..4193
                     /gene="ruvC"
                     /locus_tag="OO470_002731"
     CDS             3672..4193
                     /gene="ruvC"
                     /locus_tag="OO470_002731"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6251929.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4195..4797)
                     /locus_tag="OO470_002732"
     CDS             complement(4195..4797)
                     /locus_tag="OO470_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6251930.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4866..4931
                     /gene="yobI"
                     /locus_tag="OO470_002733"
     CDS             4866..4931
                     /gene="yobI"
                     /locus_tag="OO470_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6251931.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5070..5681
                     /gene="ruvA"
                     /locus_tag="OO470_002734"
     CDS             5070..5681
                     /gene="ruvA"
                     /locus_tag="OO470_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6251932.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5690..6700
                     /gene="ruvB"
                     /locus_tag="OO470_002735"
     CDS             5690..6700
                     /gene="ruvB"
                     /locus_tag="OO470_002735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6251933.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="OO470_002736"
     CDS             complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="OO470_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6251934.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="OO470_002737"
     CDS             complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="OO470_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6251935.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8463..9395
                     /gene="znuA"
                     /locus_tag="OO470_002738"
     CDS             8463..9395
                     /gene="znuA"
                     /locus_tag="OO470_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6251936.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9411..10733
                     /gene="mepM"
                     /locus_tag="OO470_002739"
     CDS             9411..10733
                     /gene="mepM"
                     /locus_tag="OO470_002739"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6251937.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10853..11824
                     /gene="lpxM"
                     /locus_tag="OO470_002740"
                     /gene_synonym="msbB"
     CDS             10853..11824
                     /gene="lpxM"
                     /locus_tag="OO470_002740"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6251938.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="OO470_002741"
     CDS             complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="OO470_002741"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6251939.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="OO470_002742"
                     /gene_synonym="yebK"
     CDS             complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="OO470_002742"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6251940.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14731..16206
                     /gene="zwf"
                     /locus_tag="OO470_002743"
     CDS             14731..16206
                     /gene="zwf"
                     /locus_tag="OO470_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6251941.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16441..>17044
                     /locus_tag="OO470_002744"
     CDS             16441..>17044
                     /locus_tag="OO470_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6251942.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGK"
BASE COUNT         4350 a         4203 c         4432 g         4059 t
ORIGIN      
        1 ggcatcaacc ggctgtttta atgcttcggc gtaatcggca gaccagccaa ctcgcaccag
       61 aaacacgggc tgaccgctgg cgcgaaattt cgccgccagc ttcccggcgc gattaactac
      121 ctcatcggca gtatgtggac caccggcaaa aggtaagatg ccttcttgta aatcgatcac
      181 caccaacgca gtggttttag cattaagttc aagcatgctg actcctgaaa aaacaaaatc
      241 gtgcctcaca ccttaatgcg attagccgat ggcggaacga caaattttgt taatttttat
      301 gagttcgggc aacaccttta tagcaaactg gcgtagagtc tgatttcagg cccgatataa
      361 agtggtaacg aggcgttcca gcgcgggcca ggctcttacg gtgcggcaga atttccagta
      421 taatagccgc cttttttcat tcagttgtga catacagcta acgctgcgac ttggtcacct
      481 gcgcagcagg gggcatccgc cggcagctaa gttaagggat atctcatgcg tacagaatat
      541 tgtggacagc tccgtttgtc ccacgtgggg cagcaggtga ctctgtgtgg ttgggtcaac
      601 cgtcgtcgtg atcttggtag cctgatcttc atcgatatgc gcgaccgcga aggtatcgtg
      661 caggtatttt tcgatccgga tcgtgcggac gcgttaaagc tggcctctga actgcgtaat
      721 gagttctgca ttcaggttac gggcaccgta cgtgcgcgtg acgaaaaaaa tattaaccgc
      781 gatatggcga ccggcgaaat tgaagtgctg gcgtcctcgc tgactatcat caaccgcgca
      841 gatgttctgc cgcttgactc taaccacgtc aacaccgaag aagcgcgtct gaaataccgc
      901 tacctcgacc tgcgtcgccc ggaaatggcg cagcgcctga aaacccgcgc taaaatcacc
      961 agcctggtgc gccgttttat ggatgaccac ggcttcctcg acatcgaaac tccgatgctg
     1021 accaaagcca cgccggaagg cgcgcgtgac tacctggtgc cttctcgtgt gcacaaaggt
     1081 aaattctacg cactgccgca atccccacag ttgttcaaac agctgctgat gatgtccggt
     1141 tttgaccgtt actatcagat cgttaaatgc ttccgtgacg aagacctgcg cgctgatcgt
     1201 cagcctgaat ttactcagat cgatgtggaa acttctttca tgaccgcgcc gcaagtgcgt
     1261 gaagtgatgg aagcgctggt gcgtcatttg tggctggaag tgaagggcgt ggatctgggc
     1321 gatttcccgg taatgacctt tgcggaagca gaacgccgtt atggttctga taaaccggat
     1381 ctgcgtaacc cgatggaact gaccgacgtt gccgatctgc tgaaatctgt tgagtttgca
     1441 gtatttgcgg ggccggcgaa cgatccgaaa ggccgtgttg ccgctctacg tgttccgggt
     1501 ggcgcatcgc tgacccgtaa gcagatcgac gaatacggta acttcgttaa aatctacggc
     1561 gcgaaaggtc tggcttacat caaagttaac gaacgcgcga aaggtctgga aggtatcaac
     1621 agcccggtag cgaagttcct taatgcagaa atcatcgaag ccatcctgga tcgtactgcc
     1681 gcgcaagatg gcgatatgat tttcttcggt gccgacaaca agaaaattgt tgccgacggg
     1741 atgggcgcac tgctcctgaa agtgggtaaa gaccttggtc tgaccgacga aagcaaatgg
     1801 gcaccgctgt gggttatcga cttcccgatg tttgaagacg acggtgaagg cggcctgacg
     1861 gcaatgcacc atccgttcac ctcaccgaaa gatatgactg ctgcagaact gaaagctgca
     1921 ccggaaaatg cggtggcgaa cgcttacgat atggtcatca atggttacga agtgggcggt
     1981 ggttcagtac gtatccataa tggtgatatg cagcagacgg tgtttggtat tctgggtatc
     2041 aacgaagagg aacagcgcga gaaattcggc ttcctgctcg acgccctgaa atacggtact
     2101 ccgccgcacg caggtctggc attcggtctt gaccgtctga ccatgctgct gactggcacc
     2161 gacaatatcc gtgacgttat cgccttcccg aaaaccacgg cggcagcgtg tctgatgact
     2221 gaagcaccga gctttgctaa cccgactgca ctggctgagc tgagcattca ggttgtgaag
     2281 aaggctgaga ataactgata tgactcaaat acacgaaatc attcgcgttg catcaaggcg
     2341 gcaactgagt gaactcccat gagcatagat aactatgtga atgggatgag cgaaggcagt
     2401 caacgaagag gcagcgtgaa ggataaagtg tataagcgtc ccgtttcgat cttagtggtc
     2461 atctatgcac aagatacgaa acgggtgctg atgttgcagc ggcgtgacga tcccgatttc
     2521 tggcagtcgg taaccggcag cgtggaagag ggtgaaaccg cgccgcaagc tgccatgcgc
     2581 gaagtaaagg aagaggttac cattgatgtt gtcgctgaac aactgacctt aattgactgt
     2641 cagcgcacgg tagagtttga aattttttca catttacgtc atcgctatgc gccgggcgtg
     2701 acgcgtaata cggaatcatg gttctgtctt gcgcttccgc acgagcggca gatcgttttc
     2761 actgaacatc tggcttacaa gtggcttgat gcgcctgctg cggcggcgct cactaagtcc
     2821 tggagcaacc ggcaggcgat tgaacagttt gtaattaacg ctgcctgaac aggcagggct
     2881 atctggagat atttttatgg caggtcatag taaatgggcc aacaccagac atcgtaaagc
     2941 tgcgcaggat gctaagcgcg gtaaaatctt cactaaaatc attcgtgagc tggtaaccgc
     3001 ggctaagctg ggcggtggcg atccggacgc taacccgcgt ctgcgtgcgg cggtggataa
     3061 agcactgtct aacaacatga cccgtgacac actgaaccgc gcaattgcac gtggtgtggg
     3121 cggtgatgat gatgcaaaca tggaaaccat catctacgaa ggttacggtc ctggcggcac
     3181 ggcaatcatg attgaatgtc tgtctgacaa ccgcaaccgt accgttgctg aagtgcgtca
     3241 cgcattcagc aaatgtggcg gtaacctcgg cactgacggt tccgtagctt atctgttcag
     3301 caaaaaaggc gtgatctcct tcgagaaagg cgacgaagac accatcatgg aagcagctct
     3361 ggaagcaggt gctgaagacg ttgtgacata tgatgacggc gcgattgatg tctacactgc
     3421 atgggaagaa atgggtaaag tgcgcgacgc tctggaagcg gcaggtctga aagcagacag
     3481 cgcggaagtt tccatgatcc cgtccaccaa agctgatatg gatgcagaaa ccgcgccgaa
     3541 actgatgcgt ctgatcgata tgctggaaga ttgcgacgac gtgcaggaag tttaccataa
     3601 cggtgaaatc tctgatgagg ttgcagcgac tctctgatga tgcctgctaa acagcaaaac
     3661 ggagacgcgt gatggctatt attctcggca ttgacccggg ttcgcgcgtg accggctacg
     3721 gcgtcatccg ccaggtaggt aggcaactgt cctacctggg tagcggatgc atacgcacca
     3781 aagtggatga tttaccgtct cgtctgaagc tcatctatgc gggcgtgacg gaaatcatca
     3841 cccagttcca gcctgattat ttcgccattg aacaagtctt tatggcgaag aacgctgact
     3901 cagccctgaa actgggccag gcgcgcggcg tggcgattgt tgcggcggtg aatcaggaat
     3961 tgccggtatt tgaatacgcg gcacgtcagg taaagcaaac ggtggtaggt atgggtagtg
     4021 ccgaaaaaag ccaggtgcag catatggtcc gcaccttgct gaaactgccc gctaatccac
     4081 aggcggatgc cgccgatgcg ctggcgattg ccatcaccca ctgccacgtt agtcagaatg
     4141 cgatgcagat gagcgaatcg cggctgaacc tgacgagagg gcgactgcgt taagttatac
     4201 cgcctcggtc agttccggct gaggcgtttc aactccctcc gcgtgaatca acaccaaacc
     4261 cggatgatgc cacaaactgg ctggcgtgac ggttttacgc tcccacggaa tagaacctaa
     4321 gaaccagaat ttccagaaag tgagttttgc attcggattg ctatttaaca gttgtccaaa
     4381 cgtctctatt tcacccaccg gaatacatag cgcctcttta taaatatcaa agacaaattt
     4441 tgaacagaac tggcgcgaag attcgtattt aaaacccgtg tggtaaagtt tgcgtagccg
     4501 ggagggaacc tgttcaacaa ttcgttgttt ttgttgttct gtcagcccag cgtctaatcg
     4561 ctttatagca tagcgttgat tagacgaacg tttaataaaa cgagatagcg tggtgatggt
     4621 tgaaagggga acccggcttt ctgcaaccag gaagtcttca ccgttatgac cgataatgat
     4681 cccgacgtga ttactccagc aatttgatgc agctgatatt tgaccaaata aggcagcacc
     4741 tatacatgta aagacgatat ctccaatttc atattcagca gggtaattaa tattcacgtt
     4801 gtcattccat tgaaatagat acgaatatac gtaatagcat aaaaaattat ttttcaggag
     4861 tcgcaatgta tatttttatt acgcatttct tcactgaata tgtaatattc aaatatttgc
     4921 ttccaatata acctgttgaa taaattatac tgtgccattt ttcagatcat cgggacacct
     4981 cgcaagtttt cttcatcctt cgctggatat ctatccagca tttttttatc atacagcatt
     5041 atctttgatt cattacgcag gagcgtcatg tgataggcag actcagaggc atcatcattg
     5101 aaaaacaacc cccgctggtg ttaattgaag tgggcggcgt aggctatgaa gtgcatatgc
     5161 cgatgacctg tttttatgaa ctccctgaag cgggtcagga agcgatcgtt ttcacccact
     5221 ttgtggtgcg tgaagacgcg caactgctgt acggttttaa caataaacaa gaacgcacgt
     5281 tgttcaaaga gttgatcaaa accaacggcg tcggcccgaa gttggccctg gcgatcctct
     5341 ccggaatgtc agcgcagcag ttcgttaatg ccgttgagcg tgaagaagtg ggggcactgg
     5401 tgaaactgcc gggtattggc aaaaaaaccg ccgaacgctt gattgttgaa atgaaagacc
     5461 gatttaaagg tttgcatggc gatctcttta cgccagccgc cgacctggta ctcacgtctc
     5521 ctgctagccc ggcgaccgac gatgctgaac aagaagcagt tgccgcgctg gtggcgctag
     5581 gctataaacc acaagaagca agccgcatgg tgagcaaaat cgctcgccct gacaccagca
     5641 gtgaaacatt aattcgcgaa gccctacgcg ccgcgttatg aggtaaagga tgattgaagc
     5701 agaccgtctg atttctgccg gtaccacttt gccggaagat gtagcagatc gcgccattcg
     5761 ccctaaatta ctggaagagt atgttggtca gccgcaggtt cgttcacaga tggagatttt
     5821 catcaaagca gcgaaactgc gcggcgatgc cctcgatcat ttgttgattt ttggtcctcc
     5881 ggggttgggt aaaactacgc ttgccaacat tgtcgccaat gaaatgggcg ttaatttacg
     5941 cacgacttct ggtccggtgc tggaaaaggc gggcgatttg gctgcgatgc tcactaacct
     6001 tgaaccgcat gacgtgctgt ttattgatga gatccaccgt ttatcgccag ttgttgaaga
     6061 agtgctgtac ccggcaatgg aagactacca actggatatc atgattggtg aaggtccggc
     6121 ggcacgctcc attaaaattg atttgccgcc gtttaccctg attggcgcaa ccacgcgcgc
     6181 aggttcgctg acatcaccgt tgcgcgaccg ttttggtatt gtgcaacgtc tggagtttta
     6241 tcaggtgccg gatctgcaat atatcgtcag tcgcagtgca cgctttatgg ggcttgagat
     6301 gagtgatgac ggtgcgcagg aagtagctcg tcgcgctcgc ggtacgccac gtattgccaa
     6361 ccgtctgctg cgtcgagtgc gtgatttcgc cgaagtgaag cacgatggca ccatctcggc
     6421 agatatcgct gctcaggcgc tggatatgtt gaatgtcgat gctgaaggtt tcgattatat
     6481 ggaccgcaaa ttgttgctgg cggtaatcga taagttcttt ggtgggccgg taggtctgga
     6541 taacctggcg gcagccattg gcgaagaacg tgaaaccatt gaagatgtac tggaacctta
     6601 tttgattcag caaggctttt tgcagcgtac accgcgcggg cgtatggcga cgacgcgggc
     6661 gtggaatcac tttggcataa cgccgccaga aatgccgtaa gtcggattgg tcgctcgcgc
     6721 cgcatccgac actggcagtg tgtctgatgc gacactgttc gcgtcttatc aggccagcca
     6781 gagatagcga accgtaggtc ggataaggcg ctcgcgccgc atccgacaaa tgtgttcagc
     6841 gatagattag ctggcctgct ttttcatcat actgataata aataacagtg ccgcacatag
     6901 caccaccgac ggacccgccg gcgtatcgta aaacgcggaa aaggttaaac cgccagttac
     6961 tgccaccatc cccaccaaaa cagcgacacc agccatctgt tccggcgtac gggcaaagcg
     7021 acgcgcagta gctgcaggaa taatcagcaa cgaagtaata atcaacgcac cgacaaattt
     7081 catcgctaca ccaatcgtca atgccgtcac cagcatcaac aacaatttca cttgctgtaa
     7141 tttcacacca tcaacaaacg ccagatccgg gctaatcgtc atcgacagca aattgcgcca
     7201 ttgccagaac aaaatagcca ccacgatgac cacgccaatc gcaatagaga tgagatcttc
     7261 tggcgtcact gccagtaaat caccgaacag gtaagccatc aaatcaacac gaatgttaga
     7321 catcagacta acgaccacta ggcccagcga cagggcactg tgcgccataa tccctaataa
     7381 cgtgtcgatc gccagctgtg gacgcttctc cagccatacc agaccgcccg ccagcagcag
     7441 cgtaacggca atcaccgcat agaatggatt cacgtccagc aacaaaccaa acgcgacacc
     7501 aagtaatgag gcatgagcca gcgtatcacc gaaataagac atacgacgcc agactacaaa
     7561 cgaacccagc ggacccgcgg cacaggcgag catgatcccg gctaaccaac cgggaaataa
     7621 taattcaatc atgagcgatc atttccccga cgcaaaacaa ttcgtccctg taaatcgtga
     7681 cgatgattat gatgatggcg atagataccc agttgttcag caccacgagg accaaacatt
     7741 gaaataaact ccggatgcag ggaaacaact tccggtgtgc cggaacaaca aatgtggtga
     7801 ttcaggcaca gcacttcatc ggtttttgcc atcaccagat gcagatcgtg agaaaccatt
     7861 aaaacgccac aatccagttc acgacgcaac tggtcaataa ggtcatataa cgccacttgg
     7921 ccatttacat ccacgccttg agtgggttca tccagcacta ataattgcgg gcaatttaac
     7981 aatgctcgcg ctaatagtac acgctgcgtt tcgccaccag agagcttttg catcggtgcg
     8041 ttaatcaggt gcccggcctg gacacgtttc agtgcaggca aaatatcttc tttatgtgta
     8101 ccagggcgta agcgtaaaaa acggtttacg gtcagtggca acgtggtgtc gagatacagc
     8161 ttctgcggta catagccgat gcgcagtttt ccgttgcgct tgataacccc ttcatcgggt
     8221 gttaccagcc cgagcactac ccgtaccagt gtcgacttac ctgcgccatt tggcccaagt
     8281 aaagtcaaaa tttttccagg tttaagttcc agcgacacat cagagaggac gcggcgttgg
     8341 ccaaaagaaa ccgagacatt ttccagggaa accagacttg tcatgttaat tttagtcttg
     8401 cagtagtcat gaaatgttat aatatcacac ttctcatatt cattacgatg attggtcgca
     8461 ttatgttaca taaaaaaacg cttcttttcg cagcattatc cgccgctctc tggggaggtg
     8521 caacacaggc cgcagatgcc gccgttgtcg cttcgcttaa acccgttggg ttcatcgctt
     8581 ctgccattgc tgatggtgta acagaaacgg aggttttact gcctgacggc gcttcagaac
     8641 atgattattc actgcgtcca tcggatgtaa aacgcttaca gaacgcggac ttagtcgttt
     8701 gggttggccc ggagatggaa gcgtttatgc aaaaaccggt aagcaaatta ccagaagcga
     8761 agcaggtaac gattgcgcag cttgaagatg tgaaaccgct actgatgaaa agtattcacg
     8821 gcgatgatga tgatcacgac cacgcggaaa aaagtgacga agatcaccat cacggcgatt
     8881 tcaacatgca tctttggctt tccccagaga tagcgcgggc tacagcggtt gcaatccatg
     8941 gaaaattagt ggaacttatg ccgcaaagtc gagccaaact tgacgccaac ctgaaggatt
     9001 ttgaggcaca attggcctca accgaaacgc aggttggtaa cgagctcgcg ccgctcaagg
     9061 ggaaaggtta tttcgttttt cacgatgctt acggctattt cgaaaaacag ttcggactga
     9121 caccgcttgg tcattttacc gttaaccctg agattcaacc tggcgcgcag cgtttacatg
     9181 aaataagaac acagttggtt gagcaaaaag caacctgcgt ttttgctgag ccacagttca
     9241 ggccagcggt cgttgagagc gtcgcacgag ggacatccgt tcgtatggga acgttggatc
     9301 ccctggggac gaatatcaaa ctgggtaaaa caagctattc agaattcctg agtcaattag
     9361 ccaaccagta tgcgagctgc ctgaaaggag attaatgagg aagtgattac gtgcaacaga
     9421 tagcccgctc tgtcgccttg gcgtttaata atttaccgcg accacaccgc gttatgttgg
     9481 ggtcgctcac cgttcttact ctggccgtcg ctgtctggcg gccctatgtt tatcaccgtg
     9541 atgccacgcc aattgtcaaa accattgagc tggaacagaa cgaaattcgt tcgctcttac
     9601 ctgaagccag tgagccgatt gatcaagctg cacaagaaga tgaagccatt ccccaggacg
     9661 aactggatga caaaatcgcc ggtgaagcgg gcgtgcatga atatgttgtt tccactggcg
     9721 atacgctaag cagcattctc aatcagtatg gtattgatat gggtgatatc acccaacttg
     9781 ctgctgccga caaagaattg cgtaacctga aaatcggtca acaactctcc tggacattaa
     9841 ccgcggacgg cgaactgcag cgcctcacct gggaagtgtc tcgtcgtgaa acccgaacct
     9901 atgaccgtac tgccgctaac ggttttaaaa tgaccagcga aatgcagcaa ggagagtggg
     9961 ttaacaatct gctgaaaggt accgtcggag ggagctttgt tgccagcgcc agaaacgccg
    10021 gtttaaccag cgccgaagtg agcgcagtga ttaaagccat gcagtggcaa atggatttcc
    10081 gcaaactgaa aaaaggcgat gaatttgcgg tattaatgtc tcgagaaatg cttgatggta
    10141 aacgtgagca aagccagctg ctgggcgtac gtttgcgttc agaaggtaaa gattattacg
    10201 caatccgcgc tgaggatggc aaattctacg atcgtaacgg tactggtctg gcgaaaggat
    10261 tcttgcgatt cccgacggcg aaacagttcc gtatctcgtc taactttaac ccgcgtcgaa
    10321 ctaatccggt gacaggtcgc gttgcaccac acagaggtgt tgatttcgcc atgccacaag
    10381 gtacgccagt gctttcagtg ggtgacggtg aagtggtggt tgccaaacgc agtggcgcag
    10441 caggttatta tgtggctatt cgtcatggtc gcagctacac cacgcgttat atgcacttgc
    10501 gcaagattct ggtgaaaccg ggacagaagg tgaaacgtgg cgaccgtatc gcgctttccg
    10561 gtaataccgg acgttcaact gggccgcatc tgcactatga agtatggata aaccagcagg
    10621 ccgtaaaccc gctgacggca aaactgccgc gtaccgaagg gctgaccggc tccgatcgtc
    10681 gcgaattcct ggcgcaggcc aaagagattg tgccgcagct acggtttgat taattaacat
    10741 ccattcgcag ccggtacgca gtcagtaccg gcttttttta tttggtgcgg ggcaagttgt
    10801 gccgctacac tatcaccaga ttgatttttg ccttatccga aactggaaaa gcatggaaac
    10861 gaaaaaaaat aatagcgaat acattcctga gtttgataaa tcctttcgcc acccgcgcta
    10921 ctggggagca tggctgggtg tagcagcgat ggcgggtatc gctttaacgc cgccaaagtt
    10981 ccgtgatccc attctggcac ggctgggacg ttttgccgga cgactgggaa aaagctcacg
    11041 ccgtcgtgcg ttaatcaatc tgtcgctctg ctttccagaa cgtagtgaag ctgaacgcga
    11101 agcgattgtt gatgagatgt ttgccaccgc gccgcaagcg atggcaatga tggctgagtt
    11161 ggcaatacgc gggccggaga aaattcagcc gcgcgttggc tggcaagggc tggagatcat
    11221 cgaagagatg cggcgtaata acgagaaagt tatctttctg gtgccgcacg gttgggccgt
    11281 cgatattcct gccatgctga tggcctcgca agggcagaaa atggcagcga tgttccataa
    11341 tcagggcaac ccggtttttg attatgtctg gaacacggtg cgtcgtcgct ttggcggtcg
    11401 tctgcatgcg agaaatgacg gtattaaacc attcatccag tcggtacgtc aggggtactg
    11461 gggatattat ttacccgatc aggatcatgg cccagagcac agcgaatttg tggatttctt
    11521 tgccacctat aaagcgacgt tgcccgcgat tggtcgtttg atgaaagtat gccgtgcgcg
    11581 cgttgtaccg ctgtttccga tttatgatgg caagacgcat cgtctgacga ttcaggtgcg
    11641 cccaccgatg gatgatctgt tagaggcgga tgatcatacg attgagcggc ggatgaatga
    11701 agaagtcgag atttttgttg gtccgcgacc agaacaatac acctggatac taaaattgct
    11761 gaaaactcgc aaaccgggcg aaatccagcc gtataagcgc aaagatcttt atcccatcaa
    11821 ataaaaaagc ctctcgcgag gagaggcctt cgcctgatga taagttcaag tttgcttcag
    11881 aatattcgaa atctgttgaa ctatcattga actataggcc ggatgtggcg ttttcgccgc
    11941 atccggcaac gtacttactc taccgttaaa atacgcgtgg tattagtaga acccacggta
    12001 ctcatcacgt cgccttgggt gacaatcacc aggtcaccag acatcaagta acctttatcg
    12061 cgcagcagat taaccgcttc gctggcagct gctacgccgt cattagcgct atcaaagtgc
    12121 accggcgtaa cgccacgata gagagcagtc aggttcagcg tacgttcatg gcgcgacatg
    12181 gcgaaaattg gtagaccaga gctgatacgg gaggtcatca gcgcggtacg acccgattcg
    12241 gtcatggtaa tgatcgccgt aacgcccttc aggtggttag ctgcgtacat tgccgacatg
    12301 gcaatagctt cttccacatt gtcgaactga acgtccagac ggtgtttaga aacgttgatg
    12361 ctcgggattt tttccgcacc caggcaaacg cgcgccatgg ctgcaacagt ttctgacgga
    12421 tactgcccag cggcagtttc tgcagacagc atcacagcgt cagtaccatc cagaacggcg
    12481 tttgctacgt ccatgacttc tgcacgcgtc ggcatcgggt tagtaatcat tgactccatc
    12541 atctgggtcg ccgtgattac cgctcggttt agctgacgcg cacgacggat caacgctttc
    12601 tgaatgccga ccagttccgg gtcgccaatt tccacaccga ggtcgccacg tgcaaccatt
    12661 accacgtcag aggcgaggat gatgtcatcc attgcatcct ggctgcaaac ggcttccgca
    12721 cgttcaacct tggcaacaat tttcgcatca catcctgcat cgcgtgccag gcgacgggca
    12781 tagttcagat cttcgccaca gcgtgggaag gagacagcca ggtaatctac gccaatcaac
    12841 gccgcagtct taatgtctgc tttgtctttt tcggtcagcg cttcagccga caaaccgccg
    12901 ccaagtttgt tgataccttt attgttggag aggggaccac cgacggtgac ttcggtgaac
    12961 actttcatgc cctgaacttc cagtactttt aactggacgc gaccatcgtc cagcagcagg
    13021 atgtcaccag gcacgacgtc agcaggcagg cctttgtagt cgataccgac tttttctttg
    13081 tcgccttcac ctttacccag gttggcgtcg agcaggaatt tatccccaat attgaggaaa
    13141 actttgcctt ctttaaaggt ggatacacgg attttgggcc cctggaggtc acccagaata
    13201 gccacatgac gccccagttt tgcggcaatc tcacgaactt tatccgcgcg cattttgtga
    13261 tcttcaggcg agccgtgaga aaagttcata cgtacaacgt tggcacccgc cgcgataact
    13321 ttttcaagat tattatcgcg atctgttgct gggcctaacg tggtaacgat ttttgttctg
    13381 cgaagccttt tggacatgta atactccgtt gactgaaaca accaggtgtt gcttgaacat
    13441 gaaatccgaa tgaaataacg ccgcgatggc gataaattaa tctcattcgg atgtcgtata
    13501 agattaggac agtgacagtc gcttttagcg atcgtcactt aaattaagta actgcttatc
    13561 aaaacgcgat tccttcagcg cttccttgac ccgcttcaag ttatctctga attttgcacc
    13621 gcgtcgcaaa gtaaatcctg tcgccagcac atctatcacg gtcagctgtg caagtcgaga
    13681 aaccatgggc atataaatgt cagtatcttc cggtacgtcg agggtaattg ccagcgttgc
    13741 ttcccgggcg agcggggtac ccgcagaggt gagggcaatc accatggcgt cgttttcgcg
    13801 tgccagctgc gccagctcga ccagattttt tgttcttcct gtgtgagaaa tcagcaccac
    13861 cacgtctccg tcgctacaat tcatacaact catgcgttgc agcacgatat catcggagta
    13921 caccaccgga acattaaaac gaaagaactt attcatcgca tcgtgggcaa cggcggcaga
    13981 agagcctaat ccgaaaaagg cgattttttt tgcctgagtg agcaagtcga cggcgcggtt
    14041 gatggcagat ttatccagtg aatgacggac atgatcgagc gttgccattg cggactcaaa
    14101 tattttcccc gtgtatgatt caacgctgtc atcttcattg acgttgcgat taacataggg
    14161 agtgccattc gccagacttt gtgccagatg aagtttaaaa tcaggaaaac cgcgcgtgtc
    14221 catgctgcga cagaaacgat tcaccgtcgg ttcgctaaca ttggcttcca gcgccagagc
    14281 agcaatactc gaatggatcg cgttatcggg cgaagccaga atgacctcgg caactttgcg
    14341 ctctgatttg ctcaaatgtt ccagctgaga ctggattttt tccagcatat tcatgatgta
    14401 aagagactca cgggtaatga cgatttccgc actgaaagaa atcgaaatgc agttttgtca
    14461 gatattacgc ctgtgtgccg tgttaatgac aaaagcagat aaaaaagttg ttattttttt
    14521 cataacatga tcagtgtcag atttttaccg aatggaaaac gatgattttt ttatcagttt
    14581 tgccgcactt tgcgcgcttt tcccgtaatc gcacgggtgg ataagcgttt acagttttcg
    14641 caagctcgta aaagcagtac agtgcaccgt aagaaaatta caagtatacc ctggcttaag
    14701 taccgggtta gttaacttaa ggagaatgac atggcggtaa cgcaaacagc ccaggcctgt
    14761 gacctggtca ttttcggcgc gaaaggcgac cttgcgcgtc gtaaattgct gccttccctg
    14821 tatcaactgg aaaaagtcgg tcagctcaac ccggacaccc ggattatcgg cgtagggcgt
    14881 gctgactggg ataaagcggc atataccaaa gttgtccgcg aggcgctcga aactttcatg
    14941 aaagaaacca ttgatgaagg tttatgggac accctgagtg cacgtctgga tttttgtaat
    15001 ctcgatgtca atgacactgc tgcattcaac cgtctcggcg cgatgctgga tcaaaaaaat
    15061 cgtatcacta ttaactactt tgccatgccg cccagcactt ttggcgcaat ttgcaaaggg
    15121 cttggcgagg caaaactgaa tgctaaaccg gcacgcgtag tcatggagaa accgctgggg
    15181 acgtcgctgg cgacctcgca ggaaatcaat gatcaggttg gcgaatactt cgaggagtgc
    15241 caggtttacc gtatcgacca ctatcttggt aaagaaacgg tgctgaacct gttggcgctg
    15301 cgttttgcta actccctgtt tgtgaataac tgggacaatc gcaccattga tcatgttgag
    15361 attaccgtgg cagaagaagt ggggatcgaa gggcgctggg gctattttga taaagccggt
    15421 cagatgcgtg acatgattca gaaccacctg ctacaaattc tctgcatgat tgcgatgtct
    15481 ccgccatctg acctgagcgc agacagcatc cgcgatgaaa aagtgaaagt actgaagtcc
    15541 ctgcgccgca tcgaccgctc caacgtacgc gaaaaaaccg tacgcgggca atatactgcg
    15601 ggcttcgccc agggcaaaaa agtgccggga tatctggaag aagagggcgc gaacaaaagc
    15661 agcaatacag aaaccttcgt ggcgatccgc gtcgacattg ataactggcg ctgggccggt
    15721 gtgccattct acctgcgtac tggtaaacgt ctgccgacca aatgttctga agtcgtggtc
    15781 tatttcaaaa cacctgaact gaatctgttt aaagagtcgt ggcaggatct gccgcagaat
    15841 aaactgacta tccgtctgca acctgatgaa ggcgtggata tccaggtact gaataaagtt
    15901 cctggccttg accacaaaca taacctgcaa atcaccaagc tggatctgag ctattcagaa
    15961 acctttaatc agacgcatct ggcggatgcc tatgaacgtt tgctgctgga aaccatgcgt
    16021 ggtattcagg cactgtttgt acgtcgcgac gaagtggaag aagcctggaa atgggtagac
    16081 tccattactg aggcgtgggc gatggacaat gatgcgccga aaccgtatca ggccggaacc
    16141 tggggacccg ttgcctcggt ggcgatgatt acccgtgatg gtcgttcctg gaatgagttt
    16201 gagtaatatc agcgcttatc ctttatggtt attttaccgg taacatgatc ttgcacagat
    16261 tgtagaacaa tttttacact ttcaggcctc gtgcggattc acccacgagg ctttttttat
    16321 tacactgact gaaacgtttt tgccctatga gctccggtta caggcgtttc actcataaat
    16381 cctctgaatg aaacgcgttg tgaatcatcc tgctctgaca actcaatttc aggagccttt
    16441 atgaatccac aattgttacg cgtaacaaat cgaatcattg aacgttcgcg cgagactcgc
    16501 tctgcttatc tcgcccggat agaacaagcg aaaacgtcga ccgttcatcg ttcgcagttg
    16561 gcatgcggta acctggcaca cggtttcgct gcctgccagc cagaagacaa agcctctttg
    16621 aaaagcatgt tgcgtaacaa tatcgccatc atcacctcct ataacgacat gctctccgcg
    16681 caccagcctt atgaacacta tccagaaatc attcgtaaag ccctgcatga agcgaatgcg
    16741 gttggtcagg ttgcgggcgg tgttccggcg atgtgtgatg gtgtcaccca ggggcaggat
    16801 ggaatggaat tgtcgctgct aagccgcgaa gtgatagcga tgtctgcggc ggtggggctg
    16861 tcccataaca tgtttgatgg tgctctgttc ctcggtgtgt gcgacaagat tgtcccgggt
    16921 ctgacgatgg cagccctgtc gtttggtcat ttgcctgcgg tgtttgtgcc gtctggaccg
    16981 atggcaagcg gtttgccaaa taaagaaaaa gtgcgtattc gccagcttta tgccgaaggt
    17041 aaag
//
LOCUS       DAJTUN010000089        16515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTUN010000089 DAJTUN010000000
VERSION     DAJTUN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.089"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(83..2524)
                     /gene="rnr"
                     /locus_tag="OO470_002745"
     CDS             complement(83..2524)
                     /gene="rnr"
                     /locus_tag="OO470_002745"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6251943.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2563..2988)
                     /gene="nsrR"
                     /locus_tag="OO470_002746"
     CDS             complement(2563..2988)
                     /gene="nsrR"
                     /locus_tag="OO470_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6251944.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3193..4491)
                     /gene="purA"
                     /locus_tag="OO470_002747"
     CDS             complement(3193..4491)
                     /gene="purA"
                     /locus_tag="OO470_002747"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6251945.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4595..4792)
                     /locus_tag="OO470_002748"
     CDS             complement(4595..4792)
                     /locus_tag="OO470_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6251946.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4874..5878)
                     /gene="hflC"
                     /locus_tag="OO470_002749"
     CDS             complement(4874..5878)
                     /gene="hflC"
                     /locus_tag="OO470_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6251947.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5881..7140)
                     /gene="hflK"
                     /locus_tag="OO470_002750"
     CDS             complement(5881..7140)
                     /gene="hflK"
                     /locus_tag="OO470_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6251948.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7226..8506)
                     /gene="hflX"
                     /locus_tag="OO470_002751"
     CDS             complement(7226..8506)
                     /gene="hflX"
                     /locus_tag="OO470_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6251949.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRACTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8582..8890)
                     /gene="hfq"
                     /locus_tag="OO470_002752"
     CDS             complement(8582..8890)
                     /gene="hfq"
                     /locus_tag="OO470_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6251950.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8976..9926)
                     /gene="miaA"
                     /locus_tag="OO470_002753"
     CDS             complement(8976..9926)
                     /gene="miaA"
                     /locus_tag="OO470_002753"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6251951.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9919..11766)
                     /gene="mutL"
                     /locus_tag="OO470_002754"
     CDS             complement(9919..11766)
                     /gene="mutL"
                     /locus_tag="OO470_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6251952.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11776..13113)
                     /gene="amiB"
                     /locus_tag="OO470_002755"
     CDS             complement(11776..13113)
                     /gene="amiB"
                     /locus_tag="OO470_002755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6251953.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13132..13593)
                     /gene="tsaE"
                     /locus_tag="OO470_002756"
     CDS             complement(13132..13593)
                     /gene="tsaE"
                     /locus_tag="OO470_002756"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6251954.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13565..15112)
                     /gene="nnr"
                     /locus_tag="OO470_002757"
     CDS             complement(13565..15112)
                     /gene="nnr"
                     /locus_tag="OO470_002757"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6251955.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15111..16250
                     /gene="queG"
                     /locus_tag="OO470_002758"
     CDS             15111..16250
                     /gene="queG"
                     /locus_tag="OO470_002758"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6251956.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16233..16286)
                     /locus_tag="OO470_002759"
     CDS             complement(16233..16286)
                     /locus_tag="OO470_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251957.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3750 a         4716 c         4145 g         3904 t
ORIGIN      
        1 ataaggcgtt cacgccgcat ccggcatgaa caaagcgcat tttgtcagca atctaaccct
       61 ctttttttaa agagggtatt gatcactctg ccactttttt cttcgccgca cgcttcgctt
      121 tggtcgctgc ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt
      181 tcttcgccgc tttcggcttc gtttttttct caccgcggaa ggcgctgtct ggctcaaagt
      241 ttaccttttt accgacctga cgacgcttgc cgcctttttt gcctgcatcg ccttttttcg
      301 ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg ttcactggag atcagactaa
      361 agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca
      421 ggcgataagt ctggccgctg gattccccca tcaggcgttg ccctacctgg tcaaagcgat
      481 agtagtcatt gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac
      541 ggacgaagaa gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct
      601 ggtcgagcat gaagtcacac ttcagccagt cagcgacatc gcgcgttgct tcgtcggcac
      661 gacgttccgc catcgaacag tgctgaccca gttgcaacat ctcttccatc gaataatggt
      721 agccgccggt ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatatttaa
      781 tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg
      841 actgcaatgc cagaccgaag tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg
      901 agcgtagcag catggtttgc agcatttctg catcaggacg gtcggcaacc gactccagca
      961 gttccgcgta gtcacgcggt tccggcttgt taccacccgg cagctccagc cccagctccg
     1021 ccagcactga acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca
     1081 gtgccggttc tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc
     1141 actcttcaat taatttgtgc gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc
     1201 gttcagcgtt gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt
     1261 cacgggcttt atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg
     1321 gggcgtactg ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg
     1381 tcagacgcgc gtgagagctc atcaccgctt cgtagaattt gtagcccgtc aggcggcctt
     1441 tcgacgaaac cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgaac
     1501 acaggccgtt agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg
     1561 acgtgccacg gttacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga
     1621 cgtcggcaat cgcgacccat aaacgccagc cgccgccgcg ttttttctcg cagtaaactg
     1681 catcgtcaaa gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat
     1741 cgacacggcc cgcttttgct tcttccggca cttcttcttt cagccccgca acctgttgct
     1801 caacagcctg cggccagata tacggaattt catgggtacg cagagcgata tcaaccgcca
     1861 tgccggtgcc catattgtcg cccagcactt cgacgatttt acccaccgct ttggtgcggc
     1921 gagtcggacg ctgagtcagt tcgactacga ccacaaagcc catccgcgcg cccatgatct
     1981 gatcgggcgg gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga
     2041 cgcccgcttc ggtaaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga
     2101 caatacgcgc ttcacgacga cctttacggt cagcacccag cggctgcgcc agcacctgat
     2161 cgccatgaat gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt
     2221 caacccgcag aaagccgtag ccatcacggt gaccaataac ggtacctttc accaggtcga
     2281 ggcgttccgg cagcgcatag cactgacgac gagtgaagac cagttgacca tcgcgctcca
     2341 tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta
     2401 ccgccagctc atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt
     2461 cccgactagg gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg
     2521 acatctcggt tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa
     2581 tttataaagc ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt
     2641 aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg
     2701 gcaaaactca ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc
     2761 aatacgtatc gcactcgccg gtttacccag gcgaatgccg ccattttttc cacgaacagc
     2821 agtcacgtag ccggcacgac taagttgatt gattattttg accatatgat tacgggagac
     2881 gccgtagacg tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat
     2941 gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa
     3001 agggaaatcg ggaaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct
     3061 aatttatttc agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta
     3121 atggcttaac cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca
     3181 ggcgtaccag aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg
     3241 accggtagag atgatatcga tcggcacacc ggtcagctct tcaatacgct tgatgtagtt
     3301 cagcgccgcc tgcggcaggc cgctacgatc tttcacgccg aaagtggatt cagaccagcc
     3361 cggcatggtt tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt
     3421 agtcacttcg cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc
     3481 atccagaacg tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg
     3541 acgaacggca acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcaccgaa
     3601 ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca aacagttcgg ccgggaaagg
     3661 acctgcacct acacgagttg agtaagcttt gaggataccc agaacgtaat caacataacg
     3721 cgggcccagg ccggaaccgg tcgccacgcc accagcagtg gtgttggaag aagttacgta
     3781 cggataagta ccgtggtcga tatccagcag cgtaccctgc gcaccttcga acatgacgaa
     3841 atcgccacgc tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag
     3901 gatgtcggca acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt
     3961 gtagtagtta accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa
     4021 ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata
     4081 agcaggcccg ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg
     4141 cgcgttatcc agagcaacgt gataatcaag gatcagcgga catgcttcag acagcagcag
     4201 acgctcacga acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcggc
     4261 cggagacagc acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga
     4321 tggaataaga tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg
     4381 accgccctgg tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc
     4441 tttaccttcg tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa
     4501 tcaccgtttg cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc
     4561 actcaaaaat gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt
     4621 agtagaccac aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg
     4681 gcaaattggt catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta
     4741 aaccttccag taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc
     4801 cttatagaaa aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac
     4861 cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc
     4921 tatccgggct catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca
     4981 ggctacggat gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg
     5041 ctgcttcggc atcgccttca ccacgcataa tgcggccctg acgctcagct tctgccagcg
     5101 ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg
     5161 aacggtgacg acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag
     5221 acacttcggt cggcaggttg atctgcttga tacgcacatc gacaacttca atacccagcg
     5281 ccgccatact gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct
     5341 ctgccgcttc ggcaatggcg ttatctgctg ccggggtagt aacttcatct tctgtacccg
     5401 cagaaccgga gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga
     5461 cgatatcttt cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt
     5521 tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag taacggctga
     5581 aatcgctgat gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca
     5641 caaagcggtc ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt
     5701 caatgaacgg tatcttgaaa tgcagaccag gctcataaac caaaggtttg ttgtcatcgt
     5761 cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga
     5821 cagacatgta aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg
     5881 ttattccccc tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat
     5941 atcgccctga ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg
     6001 cggcagacgc agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc
     6061 tttcagcatc tggtctaacg gcagaaccat caggttgcca cctttatcgt taaccagcac
     6121 tttgcgggtg ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat
     6181 ttccggcgcg gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc
     6241 ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc
     6301 gttcgcacgc ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc
     6361 gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc
     6421 agcctggaag ttgacgtcca gcagcgtgat acccatgtca tacggacgaa tcgtctcttc
     6481 cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat
     6541 ggtgtattta ccgataactc cacgcagggc gctgtcggta gcctgacgca ggctgtcatc
     6601 cgggctggtc acgctataca gatatttttc cggattggtg acgcggtact gcacgttcat
     6661 ctcaacgcgc actacgttct cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg
     6721 cacggcttcc acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc
     6781 cggctcaacc agatggctga atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt
     6841 aatggtatag aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac
     6901 gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt
     6961 gcctttaccg ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa
     7021 atcaggtggc ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg
     7081 tttgctgctt ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat
     7141 gtttgtgctc catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg
     7201 actccacgct tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt
     7261 tacagaggcg acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct
     7321 cctccatcca ctctttttct attgcctgaa gctgataaaa acgactcctc agacgccctt
     7381 cctgcggtgg cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag
     7441 cctgaaaaag ctgtggtatc cccgctccgg tctgtgcgga aagccagaca cggatcggtt
     7501 tgttctcttc gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca
     7561 tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt
     7621 cgatgttttc ttgtacacgc acatccgccg catcaatgac gtgcagcagt aatgtggctt
     7681 gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa
     7741 taaaccctac ggtatctgca agtacggttt caccgacatc tgcaacgtca atacgccgca
     7801 acgtcgggtc gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc
     7861 gattgaaaag ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt
     7921 cggctttgat acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg
     7981 actgtatctg cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac
     8041 ccggaccacg caaacctatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca
     8101 ggcgcgtagc cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac
     8161 acgcacgttg ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca cgacactcgc
     8221 acaaacgctc caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg
     8281 aagcacccat cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat
     8341 actttgggtg cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg
     8401 aagagaccag agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga
     8461 tgtgtaccag taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct
     8521 aaatagatca gcggggaacg caggatcgct ggctccccgt gtaaaaaaac agcccgaaac
     8581 cttattcggt ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat
     8641 ggtagttact gctggtaccg ccaccggcgt tgttactgtg atgagaaacc gggcgagatg
     8701 ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga
     8761 tcacgaactg atcaaaagac tcgatttgcc cttgcagctt aataccattc accaaataaa
     8821 tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc
     8881 ccttagccat tctctctttt ccttatatgc ttatttgtac tttgaacctt tcgattctga
     8941 aaaaattgcg cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc
     9001 tgtaatactt cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc
     9061 caaccacgca gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga
     9121 taaaccattt catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc
     9181 acgcaacgaa tggaaggcaa gtccgtatgc aaatctcctc gggcaaaaag cgcccggact
     9241 tctgcttcaa aacctgaagc caacatctga tgaaaacgct gctcaatgcg ttgatggagc
     9301 agttcacggc tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct
     9361 gacgtttgcg tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg
     9421 gaaagccttt gtggatcatt tggatgaatc cttgctgccg caaccggatc tacctcctga
     9481 agttgacgat gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgc
     9541 acttccgggt ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac
     9601 atcgtaccgc ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc
     9661 agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatatccagc
     9721 aatcggtgcg gcgcggcgag taactcttca gcgttcggct tcgccgtccc gataaccatc
     9781 cctttgtaaa taagggcaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc
     9841 tcaatggcta acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc
     9901 aggctcgcct tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac
     9961 agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc
    10021 cgccagcagg gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg
    10081 tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat
    10141 cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat
    10201 ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgctaggg cagactgcgc
    10261 tttttctaat gccgattttt cttcgccaga aacttttagc cgcaacggaa tcagcagcgg
    10321 ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc aacgttctgc
    10381 cactggcaag gataaaagtg aaatgttgcc atcgcgctcc agcaacgcac agtcggaatg
    10441 gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg
    10501 ttccggcgct tttggttttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc
    10561 accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg gacgactgcc
    10621 tgaggcgggc gcaggagagt agcgcggagc taccggctca cgagctgccg gttctgcaaa
    10681 gtgattgcgc cccgccgcca cgcggttttc cgggatggca cgcggtgcgg gttgtggttc
    10741 atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca cgccctgata
    10801 gataaaatca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cggggtgtac
    10861 gttgacgtcc acctgatgcg ggtcgatctc cagatacaac acaaatgccg gttgctgatc
    10921 ggcccccagt ttgtcttcgc aggcctggcg gatcgcatga ttgatcaggc gatcgcgcat
    10981 catgcgacca ttcacgtagc aatactgaat ttccgccagt gcgggcgtag tgtgatttgg
    11041 atcggccacc cagccgcgca gggtgagatc gccgtgttgc cattcaatcg ccagcgcttg
    11101 ttcgagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg
    11161 cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg tgacgtcgaa
    11221 acgcgccagc gcaatgcggc ggatgatttc atcaatatgg ctaaattcgg ttttctcggt
    11281 gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct ccagcgtcgt
    11341 tcccaccgga tgcgctgccg gtttaaccgt cacgtccatg tcgcgtcctt cggcataggc
    11401 ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact
    11461 gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga gatcgtccag
    11521 agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat cttttttgat
    11581 accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga tatcaatatc
    11641 gatacgcgtc gcacctgcat cgaggctgtt ttccactagt tctttgacta ccgacgcagg
    11701 tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat
    11761 tggcatcaat cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggcagt
    11821 ttgtgccgtt gcaccctgcg gcgcagattg catcggatgc gcaaggaagt aattgcgcag
    11881 gcctttgtaa atggcttctg ccagctgttg ttggtaatcg tcgctcgcca gcaaacgttc
    11941 ttcgctgttg ttgctgataa aaccggtttc gaccagtact gatgggatat ccggcgagcg
    12001 cagaacgcca aggctggcgt gttctggtcg acgtttatgt atttcgccaa tgcgttgcaa
    12061 ctgactgatc atactggtcg ctacatcata ccctacccgc tgggaatgac cgaactgtaa
    12121 atccagcacc gcctggctta aataggggtc agactgactg ttcgccagca catcacccgc
    12181 cccacccagc agctccgaca gtttctcgta ctgctccagc cagctggcca tttcactgtt
    12241 ggcgcgacgg ttagagagca cccatacgga agcgccagtc gcactacggt tcggtgcggc
    12301 atcagcgtga atcgacacga ggaaattggc gttttgctta tgtgccacat cactgcgccc
    12361 catcaccgag ataaagtaat ccccgtcacg ggttaaaacg cctttaaaca tcggatcgtc
    12421 attgagcaaa gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc
    12481 accggggccg atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat
    12541 tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc gttaccgtat tactgctgat
    12601 aacacccgta gtgcggttac tttccgtttt aaacggattg cgcgccggtt cgctgacgcg
    12661 cggtgcgaca accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg gaggaggcgg
    12721 tggcggcaca tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat tctgccgctt
    12781 caccgcttcg gttttaccgt tttcggtaag atcgaccacc agccgtagcg tttgtgcatc
    12841 tttaggcgtt ccagagcgaa tcgccttcac cagattattg ccgctgaaca acaacggcag
    12901 tccctgaatc acgcccgttt gtttgatatc gagcgccacg gtgcgtttgc tttgatggct
    12961 aaacgcataa tcaggatcgc caataaaact caacgttatc cgcgcctgtt gattaccgtt
    13021 agaaacctga atatcagaga gcgtcgcggc acccaccggc gtgcacagca gcagcagcgt
    13081 cgctaccaac caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc
    13141 taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc
    13201 ttgtgcctga taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg
    13261 ttgtggccac tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa
    13321 ctccagctcc tcgggatcgg caaggcggta caaatcaaag tgatagacca ttaagttgtc
    13381 gagcgtatag ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag
    13441 agcctgtaaa aagccccggc taaaggtggt tttacctgcg cctaaatcgc catacagata
    13501 gattacggtt gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttgcctg
    13561 ctcatcaggg agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa
    13621 caatacgctg tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg
    13681 ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc tgcacaggct gcatcatacg
    13741 gcgacagttt ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc
    13801 cgctcgccat gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga
    13861 ccacggttcc ggcacctttc agcaccgcta cgccgccata acgttgtacc agacgtttgg
    13921 cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca gcctaacaac cgtgcggcct
    13981 cgctaggatg cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt
    14041 tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagggctt
    14101 ttttccccca ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca
    14161 ggctttcggt aagagagtcc atagtcagtt catgcaccat caattctggt cgtgcagtca
    14221 gcagcggcgc aatgttctca ctgcgggtca gtactcggac taaaccagca ccagcacgta
    14281 gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccgataatca
    14341 ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg cggtttcagc cagtgagaaa
    14401 gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc tgccagccag ctatccagcc
    14461 ccagtgagtc aaaatgcagt tgtcctgtaa catcccgcgc ttttccagtg agcaagccta
    14521 gtttcagcgc aataaaagtg atggtgtgat cggcgttgat cactgcgcct ggcgtagcgc
    14581 cggtttcagc cagcaggccg gaagggatat caaccgccac aatcggcgca ggatgggaat
    14641 tagcgtggtc gattaactgg ctaatggatt cgcggggcgc ttgctgcaag ccggtaccga
    14701 gcagcgcatc aacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct
    14761 cgccacccgc gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt
    14821 cgctctcctg ggccaacaac gtgacctcaa tgccgaccgc tttggccagt cgcgcgacca
    14881 cgtagccatc gccgccgtta ttaccatgac cgcacagcac cagccagtgg cgggcgtcag
    14941 gatacgccga acgacacacc tggaatgcgg cctcgccagc gcgaagcatc agctcataga
    15001 gtgtgagccc cagcacatct gccgcctcgc gttctccgcg gcggatatcg tcggcgtacc
    15061 agacggtgtg tggtatactt acggggtttt tcttcattgt atggtccgtc atgtcagagc
    15121 ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa ctgggctttc
    15181 agcaggtagg tattaccgat accgatctca gcgagtccga gcccaaactg caagcatggc
    15241 tggacaaaca ataccacggc gaaatggact ggatggcacg tcacggtatg ctgcgcgctc
    15301 gcccccatga gttattaccc ggtacgctgc gtgtaatcag cgtgcggatg aattaccttc
    15361 ctgctaacgc cgcatttgcc agcacgctga aaaaccccaa actcggctat gttagccgtt
    15421 atgcgctggg ccgtgactat cacaaacttc tgcgcaaccg actcaaaaag ctgggcgaga
    15481 tgattcagca acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct gcgcctattc
    15541 tcgagcgccc gttagctgaa aaagctgggc tcggctggac aggtaagcac tcacttatcc
    15601 tcaatcgcga ggccggttcg ttcttctttt taggcgaatt gctggtcgat attccgctgc
    15661 ccgtggatca accagtcgag gaaggatgcg gcaaatgcgt ggcctgtatg acgatttgcc
    15721 cgaccggtgc catcgtcgag ccatataccg tcgatgctcg ccgctgtatc tcttatctca
    15781 ccatcgaact tgaaggggcg ctcccggaag agttgcgacc gttaatggga aaccgtattt
    15841 acggttgcga tgactgccag cttatctgcc cgtggaatcg ctattcgcaa ctcactacag
    15901 aagacgattt cagcccgcgt aagccgctac acgcaccgga actcattgag ttatttgcct
    15961 ggagcgaaga gaagttttta aaagtcacgg aaggatcggc gattcgccgt ataggtcacc
    16021 tgcgctggct gcgtaatatc gccgtggcat taggcaatgc cccttgggat gaaacgattt
    16081 tgacagcgct ggaaagtcgt aaaggtgagc acccacttct cgatgagcac atagcgtggg
    16141 cgattgcgca gcaaatagag agacgaaatg cgtgcatagt cgaagtgcaa ttgccgaaaa
    16201 aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga ttcatccaca
    16261 gcttgtgaat aaaaataaaa acgcattgca attcaagacg aataaaatcg tcaagtgatc
    16321 gactcgacaa aacggaatct tgatttttaa cttatttatc aataggttaa aaaataccat
    16381 tcaccgtgcg aagtttcttt gaaattaaga ttacagcctg aggctgtgga taactctgtt
    16441 tacaagagtt tgtcagagac aacaaaaacc agcaataacg catcgcgtcg ctgtagatat
    16501 tttattgaga gaaga
//
LOCUS       DAJTUN010000090        16323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTUN010000090 DAJTUN010000000
VERSION     DAJTUN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.090"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..2424
                     /gene="gcvP"
                     /locus_tag="OO470_002760"
     CDS             <1..2424
                     /gene="gcvP"
                     /locus_tag="OO470_002760"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6251958.1"
                     /translation="ATAAAEAMAMAKRVSKLKNANRFFVASNVHPQTLDVVRTRAETF
                     GFEVIVDDAQKVLDHQDVFGVLLQQVGTTGEIHDYTALISELKSRKIVVSVAADIMAL
                     VLLTAPGKQGADIVFGSAQRFGVPMGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAG
                     NTALRMAMQTREQHIRREKANSNICTSQVLLANIASLYAVYHGPVGLKRIANRIHRLT
                     DILAAGLQQKGLKLRHAHYFDTLCVEVADKAGVLTRAEAAEINLRSDILNAVGITLDE
                     TTTRENVMQLFSVLLGDNHGLEIDTLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHS
                     ETEMMRYMHSLERKDLALNQAMIPLGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQA
                     EGYQQMIAQLADWLVKLTGYDAVCMQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLI
                     PASAHGTNPASAHMAGMQVVVVACDKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHG
                     VYEETIREVCEVVHQFGGQVYLDGANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGG
                     GGPGMGPIGVKAHLAPFVPGHSVVQIEGMLTRQGAVSAAPFGSASILPISWMYIRMMG
                     AEGLKKASQVAILNANYIASRLQDAFPVLYTGRDGRVAHECILDIRPLKEETGISELD
                     IAKRLIDYGFHAPTMSFPVAGTLMVEPTESESKVELDRFIDAMLAIRAEIDQVKAGVW
                     PLEDNPLVNAPHIQSELVAEWAHPYSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFC
                     SCVPISEYQ"
     gene            2691..3433
                     /locus_tag="OO470_002761"
                     /pseudo
     CDS             2691..3433
                     /locus_tag="OO470_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(3490..4923)
                     /gene="bglA"
                     /locus_tag="OO470_002762"
     CDS             complement(3490..4923)
                     /gene="bglA"
                     /locus_tag="OO470_002762"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6251959.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            4968..5278
                     /locus_tag="OO470_002763"
                     /pseudo
     CDS             4968..5278
                     /locus_tag="OO470_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            5442..6101
                     /locus_tag="OO470_002764"
     CDS             5442..6101
                     /locus_tag="OO470_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6251960.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(6297..7277)
                     /gene="ygfZ"
                     /locus_tag="OO470_002765"
     CDS             complement(6297..7277)
                     /gene="ygfZ"
                     /locus_tag="OO470_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6251961.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            7520..7786
                     /gene="sdhE"
                     /locus_tag="OO470_002766"
     CDS             7520..7786
                     /gene="sdhE"
                     /locus_tag="OO470_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6251962.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            7767..8174
                     /locus_tag="OO470_002767"
     CDS             7767..8174
                     /locus_tag="OO470_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6251963.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(8214..8735)
                     /gene="fldB"
                     /locus_tag="OO470_002768"
     CDS             complement(8214..8735)
                     /gene="fldB"
                     /locus_tag="OO470_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6251964.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            8847..9743
                     /gene="xerD"
                     /locus_tag="OO470_002769"
     CDS             8847..9743
                     /gene="xerD"
                     /locus_tag="OO470_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6251965.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            9768..10478
                     /gene="dsbC"
                     /locus_tag="OO470_002770"
     CDS             9768..10478
                     /gene="dsbC"
                     /locus_tag="OO470_002770"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6251966.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            10484..12217
                     /gene="recJ"
                     /locus_tag="OO470_002771"
     CDS             10484..12217
                     /gene="recJ"
                     /locus_tag="OO470_002771"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6251967.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            12308..13406
                     /gene="prfB"
                     /locus_tag="OO470_002772"
     CDS             join(12308..12382,12384..13406)
                     /gene="prfB"
                     /locus_tag="OO470_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6251968.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            13416..14933
                     /gene="lysS"
                     /locus_tag="OO470_002773"
     CDS             13416..14933
                     /gene="lysS"
                     /locus_tag="OO470_002773"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6251969.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(14976..15524)
                     /gene="idi"
                     /locus_tag="OO470_002774"
     CDS             complement(14976..15524)
                     /gene="idi"
                     /locus_tag="OO470_002774"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6251970.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            15579..15650
                     /locus_tag="OO470_002775"
     CDS             15579..15650
                     /locus_tag="OO470_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251971.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(15647..15772)
                     /locus_tag="OO470_002776"
     CDS             complement(15647..15772)
                     /locus_tag="OO470_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251972.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(15774..>16323)
                     /gene="uacT"
                     /locus_tag="OO470_002777"
     CDS             complement(15774..>16323)
                     /gene="uacT"
                     /locus_tag="OO470_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6251973.1"
                     /translation="GTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILF
                     GMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGV
                     GMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKD
                     LEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         3937 a         4129 c         4415 g         3842 t
ORIGIN      
        1 gccaccgctg ccgccgaagc aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc
       61 aaccgcttct tcgtggcttc caatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt
      121 gctgaaacct ttggttttga agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag
      181 gacgtcttcg gcgtgctgtt acagcaggta ggcactaccg gtgaaattca cgactacact
      241 gcgcttatta gcgaactgaa atcacgcaaa attgtggtca gcgttgccgc cgatattatg
      301 gcgctggtgc tgttaactgc gccgggtaaa cagggcgcgg atattgtttt tggttcggcg
      361 caacgcttcg gcgtgccgat gggctacggt ggcccacacg cggcattctt tgcggcgaaa
      421 gatgaataca aacgctcaat gccgggccgt attatcggtg tatcgaaaga tgcagctggc
      481 aataccgcgc tgcgcatggc gatgcagact cgcgagcaac atattcgtcg tgagaaagcg
      541 aactccaaca tttgtacttc ccaggtactg ctggcaaaca tcgccagcct gtatgccgtt
      601 tatcacggcc cggttggcct gaaacgtatc gctaaccgca ttcaccgtct gaccgatatt
      661 ctggcggcgg gcctgcaaca aaaaggtctg aaactgcgcc atgcgcacta tttcgacacc
      721 ttgtgtgtgg aagtggccga caaagcgggc gtactgacgc gtgccgaagc ggctgaaatc
      781 aacctgcgta gcgatatcct gaacgccgtg gggatcaccc ttgatgaaac caccacgcgt
      841 gaaaacgtaa tgcagctttt cagtgtgctg ctgggcgata accacgggct ggagatcgac
      901 acgctggaca aagacgtggc tcacgacagc cgctctatcc agcctgcgat gctgcgcgac
      961 gacgaaatcc tcacccatcc ggtgtttaat cgctatcaca gcgaaaccga aatgatgcgc
     1021 tatatgcact cgctggagcg taaagatctg gcgctgaatc aggcgatgat cccgctgggt
     1081 tcctgcacca tgaaactgaa cgccgccgcc gagatgatcc caatcacttg gacggaattt
     1141 gccgaactgc acccgttttg cccgccggaa caggctgaag gttatcagca gatgattgcg
     1201 cagctggctg actggctggt gaaactgacc ggttacgacg ccgtttgtat gcagccgaac
     1261 tccggcgcac agggcgaata cgcgggcctg ctggcgattc gtcattatca tgaaagccgc
     1321 aacgaagggc atcgcgatat ctgcctgatc ccggcttctg cgcacggaac taaccccgct
     1381 tctgcacata tggcaggaat gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc
     1441 gatctgactg atctgcgcgc gaaagcggaa caggcgggcg ataacctctc ctgtatcatg
     1501 gtgacttatc cttctaccca cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc
     1561 gtgcatcagt tcggcggtca ggtttacctt gatggcgcga acatgaacgc ccaggttggc
     1621 atcacctcgc cgggctttat tggcgcggac gtttcgcacc tcaacctgca taaaactttc
     1681 tgcattccgc acggcggtgg tggtccgggt atgggaccga tcggtgtgaa agcgcatctg
     1741 gcaccgtttg taccgggtca tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc
     1801 gcagtttctg cggcaccgtt cggtagcgcc tctatcctgc caatcagctg gatgtacatc
     1861 cgcatgatgg gcgcagaagg gctgaaaaaa gcaagccagg tggcaatcct caacgccaac
     1921 tatattgcca gccgcctgca ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc
     1981 gtggcgcacg aatgtattct cgatattcgc ccgctgaaag aagaaaccgg catcagcgag
     2041 ctggatattg ccaagcgcct gatcgactac ggtttccacg cgccgacgat gtcgttcccg
     2101 gtggcgggta cgctgatggt tgaaccgact gaatctgaaa gcaaagtgga actggatcgc
     2161 tttatcgacg cgatgctggc tatccgcgca gaaattgacc aggtgaaagc cggtgtctgg
     2221 ccgctggaag ataacccgct ggtgaacgcg ccgcacattc agagcgaact ggtcgccgag
     2281 tgggcgcatc cgtacagccg tgaagttgcg gtattcccgg caggcgtggc agacaaatac
     2341 tggccgacgg tgaaacgtct ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc
     2401 gtaccgatta gcgaatacca gtaattcact gattcgagta ttttctaaag gcgcttcggc
     2461 gcctttttag tcagatgaca aagtacaaaa gtgctcagac agtcccctcg cccctttggg
     2521 gagagggtta gggtgagggg aacaggccgg cactggcgcg aatatttacc ctcaccccga
     2581 ccctctccct gaaagggcga gggggaaaag cgtgccaaca ttgaagattg agccagtttg
     2641 ttagcaatct caaagatacg tcaacgaatt aatttttctc ggaaaaacaa atggctatag
     2701 cacttgtgac cggtggcagt cgcggcatcg ggcgggcaac tgcattactg ttggcgcaag
     2761 aagggtatac ggtggcggtt aattatcagc aaaacctcca cgcggcgcag gaagtgatga
     2821 acttaataat gcaagccggt ggcaaagcat tcgtgctcca ggcggatatc agcgacgaaa
     2881 accaggtcgt tgcgatgttt acagcaatcg atcagcacga tgaaccgcta gcagcgctgg
     2941 tcaataacgc cgggatcttg tttacccagt gcaccgttga aaaccttacc gcagagcgaa
     3001 tcaaccgagt actttccacc aacgtgacgg gatattttct ctgctgccgc gaggcggtaa
     3061 aacgcatggc gcttaaaaat ggtggcagtg gcggcgctat cgtcaatgtc tcttcggtgg
     3121 cctcacggtt gggttcgcca ggggaatatg ttgattacgc ggcatcgaaa ggggcgattg
     3181 attcgttaac caccggacta tcgctggaag tcgccgcgca ggggatccgc gttaactgcg
     3241 tgcggccagg gtttatttat accgaaatgc acgccagcgc ggcgagcctg gacgcgtcga
     3301 tcgcgttaag tcgaacatcc ccatgcagcg tggtggacag gcagaagagg tcgcgcaggc
     3361 cattgtctgg ctactaagtg ataaagcctc ttacgtcacg ggaagtttta tcgatttggc
     3421 gggcgggaaa taaaacaggg aagttgtctg accggatgca acaagtattg catccggtac
     3481 ttcatcgact taaagcttct cgccgttgct ggcaatcacc tctttgtacc agttaaagct
     3541 cttcttacgt gaacgcgaca tatcgccagt accgtcgtca tgtttattca catagataaa
     3601 gccgtagcgt ttgctgtact gcccggtagt gaatgacacg cagtcgatgc agccccacgg
     3661 tgtgtagccc atcagatcca caccatcgta agtcactgct tttttcatct cttcaatatg
     3721 ggcgcgcagg tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc
     3781 gtaagcgcca aaaccgtttt cgacaataaa cagcggcctc tgataacgct catacagttc
     3841 gcaaagggca tagcgcagac ctactggatc aatctgccag ccccagtcag acgctttaac
     3901 atacgggttc ggtacgctgc cttcaaaacc agagatcgca tcgccggtgc cgccttcggc
     3961 cttcactgcg ttggtcatgt aatagctgaa accaagataa tcgcaggtgc cttcgcgcag
     4021 tacatcgaga tcgccgtctt ccattttgat attaaatccg cgacgttccc actcgttcaa
     4081 tacataggac gggtaatagc cgcatagctg cacatcggta aagacgtagc gttcgcgcat
     4141 cgactcctga gcgaacatca catcgtccgg gttacatgag tagggataga gcggcaccat
     4201 cgccagcata cagccgactt tcatctccgg gttaatgcga cgcgcggctt tcactgccag
     4261 ggcgctggcg acaaactggt gatgcagcac ctggtacatc gtctcttccg ggttgtcatg
     4321 ctcggtatac accacgccgg agcagcagta accaaacagc ggcgcacgcc agttacgctg
     4381 gttgtttatt tcgttgaagg tcatccagta tttgacttta tgcttgtagc gttcgaatac
     4441 cacttccgcg aaacggacaa agaaatcaac gaatttacgg ttggtccagc taccgtattg
     4501 ctgcaccaga tgcagcggca tttcaaagtg ggagagggtt atcaccggtt cgatgttgta
     4561 tttcagcagt tcatcgaaca gagagtcgta aaacttcagc ccttcttcgt ttggctgagc
     4621 ttcatcacct tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac atttgaagcc
     4681 catttcggca aatagcttga tgtcttcctt gtagtgacca taaaaatcaa cggcttcatg
     4741 gtttggatag tattttcctg gcaagacttc tttggtgatt tcgcgcggca cgccgtgtgc
     4801 gccaccggtc agaacgtcac aaatgctcgg tcctttgccg cctttgttcc agccgccttc
     4861 gacctgatga gcggcaacgg cgccacccca taagaaatct ttcggtaagg tgagtttttt
     4921 cactatcata ggctccagat cagtcaattc ttacaggaaa tagtaccatg cagccaaacg
     4981 acatcacttt tttcaacgtt tccaggatga cattctggct gggcgtaaaa ccatcaccat
     5041 ccgcgacgag tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga
     5101 agatgacggt tatttttgca cgattgaagt caccgcaacc tcaaccgtaa cgctggatac
     5161 actgacggaa aaacatgcag agcaggaaaa tatgaccctg cctgaactga aaaaggtcat
     5221 tgctgacatc tatcccggtc agacacaatt ttatatgatt gaatttaaat gtctttaaat
     5281 tgaactcacg cctgttagtt gaaatttcgc atttaatttc atgattttta ggagattagt
     5341 ctcaaatcag aatttatttt agctaacagg tgttcactgg aactattctc agttacgctg
     5401 gagaggtata catgtgcgtg tacgtttctc tggagtaagt tatggttcag aagcccctca
     5461 ttaagcaggg atattcgctg gcagaggaaa ttgccaacag cgtcagtcac ggcattgggc
     5521 tggtgtttgg tatcgttggg ctggtgttgc tactggttca ggcggtggat ctcaatgcca
     5581 gcgccacagc gataaccagt tacagcctct atggcggcag tatgatcctg ctgttcctcg
     5641 cttcgacgct ctatcacgcc attcctcatc aacgggcaaa aatgtggctg aagaaatttg
     5701 accattgcgc tatttacctg ttgattgccg gaacctacac gccgtttttg ctggtggggc
     5761 tggattctcc gttagcgcgc gggttgatga ttgttatctg gagcctagca ttgctgggga
     5821 ttctgttcaa actgaccatc gcgcaccgat tcaaaatttt atctctggtg acttatctgg
     5881 cgatgggctg gctgtcgctg gtggtaattt atgaaatggc agttaagctc gcggcgggca
     5941 gcgttacctt actggcggta ggcggcgtgg tttattcgct cggggtgatt ttctacgtct
     6001 gcaaacgcat tccatacaac catgccatct ggcacggctt cgtgctcggc ggtagtgtgt
     6061 gccactttct ggcgatctat ttgtatattg ggcaggcgta atgggtatgc ctttgtaggc
     6121 ctgataagac gcgtcagcgt cgcatcaggc atcggagcgc aaccgccgga tgcggcgtga
     6181 acgccttatc cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa atcccagaca
     6241 tgataagacg cgtcagcgtc gcatcaggcg ataaatgcct gatgcgatac agagatttac
     6301 tcttcgagcg aatacggcag cggctcgata tgcagtgtat tcgcatcgtc gcgtacgcgg
     6361 aagatgctgt ccggttccat atcgttattc atgacgacct gtaccacgac ctgaccatct
     6421 tccagtttta ccgcagccag caccgtaccg gtacgacgcc agttctcgcc cattttcagc
     6481 tctaagtctt caccagcttc cggcagtcgg ctgacactac ctgccagcaa ccagagcgcg
     6541 cgtttgttgg caccacggaa ttttgctcgc gccaccatct cttgtccggt ataacagcct
     6601 ttcttaaagc tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg
     6661 ctgttggcgg catcaatcac cgggaaacca gcttcaatgt ttaatgccag ccactgttgg
     6721 ctattgttca gttccgcttc accgcgcagt ttatcggtca gcatattagc agtagcttca
     6781 tcggttacga tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct
     6841 tctttgacta cctgtttttc tttcgaaggc agttcgctaa agagatttgc cagcgcggcg
     6901 cgcgcctgaa aaccggcaac accaagcagc acacgctcgt cgtctggcgc gatggtcact
     6961 ttagagaata ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt
     7021 tcaatccatg caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct
     7081 ttggcgtcgc aatgggcggc gagcaggtgc tgatcttctg ccatctggct gacatctgct
     7141 gtcacctgac cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag
     7201 tcatcaagcg tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acgtggagga
     7261 aaaggtgtaa aagccataat agagtcctga gttgcttaac ggcgtattaa tgactaatgg
     7321 taaaagagct atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc
     7381 gactgccaaa aaaatgaagc ccttgaattg ttgtgaatta atttcgcgat cgcgatggtg
     7441 tttactgata ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg gtactggatt
     7501 ttcgtgagac aggaagaaga tggacattaa caacaaagcc cgaattcatt gggcatgccg
     7561 ccgtggtatg cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtacgacag
     7621 cttaagcgat gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct
     7681 gtttaactgg ctgatgaatc acggtaaacc ggccgatgca gaactggaaa taatggtccg
     7741 actcatccag acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct
     7801 ggcgcgcaca gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca
     7861 tgccctggcc gctcagttac accccgttat ggatggtgtt actttcgctg gtggtatttg
     7921 attgcgttcg cagccagcgg tgtattaatg ctcgccaggg ggaaattcgc ttgttgatgg
     7981 acgggcgttt gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg tggatgatta
     8041 agagcggcat gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg
     8101 cagccgacag catggacgaa gccgaatggc gggatttacg gcggattttg ttgcaacaag
     8161 agacgcaaag ataaagatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg
     8221 taatgctctg ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta
     8281 aggtcatact ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc
     8341 accggtttcg ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg
     8401 cctttggtcg agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat
     8461 cccagttgat cgccaagccc atacagcgca ataattttac cttcaaggtt caggtcgtcg
     8521 agctgatccc agacggcttc ccagtcttcc tggatttcac caaaatccca ggtcgggata
     8581 cccagaatga gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt
     8641 aaggtcacca gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa
     8701 caggtgctgg aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt
     8761 ggcttttgca gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat
     8821 caaatgagtg accataaggg gcgcaagtga aacaggagct ggcacgcatc gagcagtttc
     8881 ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg
     8941 atctgtcaat gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa
     9001 gtgacgattt gcaggcatta ctggcagaac ggctggaggg cgggtataaa gccaccagct
     9061 cagcgcgttt gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc
     9121 gtgaagacga tcccagtgcg catctcgctt caccgaaatt gccccagcgt ttgccaaaag
     9181 atttaagcga agcgcaggtc gaacgtttat tacaggcgcc attaattgat cagccactgg
     9241 agctacgcga taaagccatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac
     9301 tggtcgggct gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg gtcattggta
     9361 aaggcaacaa agagcgcctg gtgccgttag gggaagaggc ggtttactgg ctggaaacct
     9421 atctggaaca tgggcgtccg tggctgctga atggtgtgtc aattgacgtg ttgtttccca
     9481 gccagcgtgc gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgccg
     9541 tgctggcggg tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca
     9601 ctcatttatt aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg
     9661 atctttccac cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc
     9721 aacagcatca cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt
     9781 ttatgttgtt tactttgtta gcggcgtttt caggctttgc tcaggctgat gacgcggcaa
     9841 ttcaacaaac gttagccaaa atgggcatca aaagcagcga tattcagccc gcgcccgtag
     9901 ctggcatgaa gacagttctg actaacagcg gtgtgttgta catcaccgat gatggtaaac
     9961 atatcattca ggggccaatg tatgacgtta gtggcacggc tccggtcaat gtcaccaata
    10021 agatgctgtt aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc
    10081 aggaaaaaca cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc
    10141 atgagcaaat ggcagactat aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc
    10201 gccaggggct ggacagcgat gcagagaaag aaatgaaagc tatctggtgt gcgaaagata
    10261 aaaacaaagc gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agctgcgacg
    10321 tggatattgc cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag
    10381 ttgtgctgag caatggcaca cttgttccgg gttaccagcc gccgaaagac atgaaagaat
    10441 tcctcgacga acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac aacagataca
    10501 acttcgtcgc cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct
    10561 gcgccgttta tacgccagcc ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa
    10621 aggtatgttg ccctggcagc aactaagcgg tgtcgaaaag gccgttgaga tcctttacaa
    10681 cgcttttcgc gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggcgcgac
    10741 cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct
    10801 ggtaccaaac cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca
    10861 tgcccgtggc gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggagt
    10921 tgagcacgct cgctcgttgg gcatcccggt tattgttacc gatcaccatt tgccgggcga
    10981 cacattaccc gcagcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc
    11041 gaaatcactg gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt
    11101 gcgcgatcag ggctggtttg atgagcgcgg catcgcaatt cctaacctgg cagaactgct
    11161 ggatctggta gcactgggga cagtagcgga cgtcgtgccg ctggacgcta ataatcgcat
    11221 tctgacctgg caggggatga gtcgcatccg tgccggaaag tgccgtccag ggattaaagc
    11281 gctgctggaa gtggcaaatc gcgatgcaca aaaactagcc gccagcgatt taggttttgc
    11341 gctgggaccg cgtctcaatg ctgccgggcg actggatgat atgtccgttg gtgtggcgct
    11401 gttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa
    11461 ccagacgcga aaagagatcg aacaaggaat gcaggttgaa gccctgaccc tgtgcgagaa
    11521 actggagcgc agccgtgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca
    11581 tcagggcgtt gtcggtattc tggcttcgcg tatcaaagag cgttttcacc gtccggttat
    11641 cgcctttgcg ccagcaggtg acggtacgct gaaaggttcc ggtcgctcca ttcaggggct
    11701 gcatatgcgt gatgcgctgg agcgattaga cacactctac cccggcatga tgctcaagtt
    11761 tggcggtcat gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca
    11821 acaacggttt ggcgaactgg ttactgagtg gctggcccct tcgctattgc aaggcgaagt
    11881 ggtgtcagac ggtccgttaa gcccggccga aatgaccatg gaagtggcgc agctgctgcg
    11941 cgatgctggc ccgtgggggc agatgttccc ggagccgctg tttgacggtc atttccgtct
    12001 gctgcaacag cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac cggtcggcgg
    12061 cggtccactg ctggatggta ttgcttttaa tgtcgatacc gctctctggc cggataacgg
    12121 cgtgcgcgaa gtgcaactgg cttataagct cgatatcaac gagtttcgcg gcaaccgcag
    12181 cctgcaaatt atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga
    12241 gcgtggattg ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa
    12301 tcagaccatg tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc
    12361 cgacgttctt agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa
    12421 acgccgagct ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta
    12481 aagagcgttc ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg
    12541 aagatgtttc tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg
    12601 aagccgttgc tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta
    12661 tgttctctgg cgaatatgac agcgccgact gttacctcga tattcaggcg gggtctggcg
    12721 gtacggaagc acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc tgggcagaat
    12781 cgcgtggttt caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat
    12841 ccgtgacgat caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg
    12901 ttcaccgcct ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac acgtcgttca
    12961 gctctgcgtt tgtttatccg gaagttgatg atgatattga tatcgaaatc aacccggcgg
    13021 atctgcgcat tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg
    13081 aatctgcggt gcgtattacc cacatcccga ccgggatcgt gacccagtgc cagaacgacc
    13141 gttcccagca caagaacaaa gatcaggcca tgaagcagat gaaagcgaag ctttatgaac
    13201 tggagatgca gaagaaaaat gccgagaaac aggcgatgga agataacaaa tctgacatcg
    13261 gctggggcag ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca
    13321 ccggggtaga aacccgcaac acgcaggccg tgctggacgg cagcctggat caatttatcg
    13381 aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg
    13441 ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc
    13501 tgcgtgagca gggaattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat
    13561 tgcacgcaga attcgacggt aaagagaacg aagaactgga agcgctgaac atcgaagtcg
    13621 ccgttgctgg ccgcatgatg acccgccgta ttatgggtaa agcgtctttc gttaccctgc
    13681 aggatgtggg tggccgcatt cagctgtacg ttgcccgtga cgatctgccg gaaggcgttt
    13741 acaacgagca gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa ggtaaactgt
    13801 tcaaaaccaa aaccggcgaa ctgtctatcc actgtaccga gctgcgtctg ctgaccaaag
    13861 cactgcgtcc gctgccggat aaattccacg gcttgcagga tcaggaagcg cgctatcgtc
    13921 agcgttatct ggatctcatc tctaacgatg aatcccgtaa cacctttaaa gtacgctcgc
    13981 agatcctctc tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa gttgaaacgc
    14041 cgatgatgca ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc caccataacg
    14101 cgctggatct cgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg
    14161 tcggtggctt cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg
    14221 tacgtcataa cccagagttc accatgatgg aactctacat ggcttacgca gattacaaag
    14281 atctgatcga gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga
    14341 cggaagtgac ctacggcgac gtgacgctgg acttcggtaa accgttcgaa aaactgacca
    14401 tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gataacttcg
    14461 actctgcgaa agcgattgct gaatctatcg gtatccacgt tgagaagagc tggggtctgg
    14521 gccgtatcgt taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct
    14581 tcattactga atatccggca gaagtttctc cgctggcgcg tcgtaacgac attaacccgg
    14641 aaatcacaga ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac ggctttagcg
    14701 agctgaatga cgcggaagat caggcgcaac gcttcctgga tcaggttgcc gcgaaagacg
    14761 caggtgacga cgaagcgatg ttctacgatg aagattacgt taccgcactg gaacatggct
    14821 taccgccgac agcaggtctg gggattggca tcgaccgtat ggtaatgctg ttcaccaaca
    14881 gccataccat ccgcgacgtt attctgttcc cggcgatgcg tccggtgaaa taagcattac
    14941 gttatgctca caaccccggc aaatgtcggg gttttttatt taagctgggt aaatgcagat
    15001 aatcgtattc tggcttcgcg atttgtcgcc tgcatcacca tccacggact gaacgcccac
    15061 ggcgtggcat caataccgcg taatacatca gctaaatcac accattgata atccatcact
    15121 tcatcatcgt tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg acacacttca
    15181 ttttccacaa tgcctctcgg atcggtggcg cggtagcgaa agtcaggata gatagattca
    15241 ggaggcgtaa tttccacgcc aagctcataa cggcaacggc ggatcaccgc gtcttcgctg
    15301 ctttctccca gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccacgctttt
    15361 ttgctcagtg cgcggcgggt aactaataat tgccccttgg cattaaacag ccaactggag
    15421 aacgcgagat gtaagagggt gtctgccgtg tgtgcggcat acttttccag cgtacccgtg
    15481 ggaactccct gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa
    15541 ttctgatcac tcatcattat agataattga agagtgaaat gattaaccaa gagagcgttt
    15601 atcgacaacc gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca
    15661 tcatatattt gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag
    15721 agagagtaaa aagcaacaac agactgaata tagcgcgcat taaaaaattc atattattct
    15781 ccatgctcat ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgacttct
    15841 aaatctttat cagagacgga ttccttcacc aggtcagcat gatgctgata gccattaaag
    15901 aagacgttca gcacaacggc gctaagggtt gcgagcataa tgccgctatg tagcagcggt
    15961 tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt catgccaacg
    16021 ccgagactga tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac
    16081 agaattcgaa tccctgtcgc cagtaccatg ccgaacatca ctagaccagc gccgcccagc
    16141 acaaattgcg gaatggaggc taccagcacc gccatttttg gcaccatgcc aaacaggatt
    16201 aatataattc ccgaagaaat acacacccag cggctatgaa cgcgcatcac gctaaccagg
    16261 ccaacgtttt gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc tatcattgtc
    16321 cct
//
LOCUS       DAJTUN010000091        16262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTUN010000091 DAJTUN010000000
VERSION     DAJTUN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.091"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..436)
                     /gene="yhaJ"
                     /locus_tag="OO470_002778"
     CDS             complement(<1..436)
                     /gene="yhaJ"
                     /locus_tag="OO470_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6251974.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGR"
     gene            541..1242
                     /locus_tag="OO470_002779"
     CDS             541..1242
                     /locus_tag="OO470_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6251975.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1265..1429
                     /locus_tag="OO470_002780"
     CDS             1265..1429
                     /locus_tag="OO470_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251976.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1641..2951)
                     /locus_tag="OO470_002781"
     CDS             complement(1641..2951)
                     /locus_tag="OO470_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6251977.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2979..4310)
                     /locus_tag="OO470_002782"
     CDS             complement(2979..4310)
                     /locus_tag="OO470_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6251978.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(4585..5949)
                     /gene="tdcG"
                     /locus_tag="OO470_002783"
     CDS             complement(4585..5949)
                     /gene="tdcG"
                     /locus_tag="OO470_002783"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6251979.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6021..6410)
                     /locus_tag="OO470_002784"
     CDS             complement(6021..6410)
                     /locus_tag="OO470_002784"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6251980.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6424..8718)
                     /gene="tdcE"
                     /locus_tag="OO470_002785"
     CDS             complement(6424..8718)
                     /gene="tdcE"
                     /locus_tag="OO470_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6251981.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8752..9960)
                     /gene="tdcD"
                     /locus_tag="OO470_002786"
     CDS             complement(8752..9960)
                     /gene="tdcD"
                     /locus_tag="OO470_002786"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6251982.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9986..11317)
                     /gene="tdcC"
                     /locus_tag="OO470_002787"
     CDS             complement(9986..11317)
                     /gene="tdcC"
                     /locus_tag="OO470_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6251983.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11339..12328)
                     /gene="tdcB"
                     /locus_tag="OO470_002788"
     CDS             complement(11339..12328)
                     /gene="tdcB"
                     /locus_tag="OO470_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6251984.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12427..13365)
                     /gene="tdcA"
                     /locus_tag="OO470_002789"
     CDS             complement(12427..13365)
                     /gene="tdcA"
                     /locus_tag="OO470_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6251985.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            13555..13899
                     /gene="tdcR"
                     /locus_tag="OO470_002790"
     CDS             13555..13899
                     /gene="tdcR"
                     /locus_tag="OO470_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6251986.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14155..14694
                     /locus_tag="OO470_002791"
     CDS             14155..14694
                     /locus_tag="OO470_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251987.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            14716..15063
                     /locus_tag="OO470_002792"
     CDS             14716..15063
                     /locus_tag="OO470_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251988.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15232..15882
                     /locus_tag="OO470_002793"
     CDS             15232..15882
                     /locus_tag="OO470_002793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6251989.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         4503 a         4135 c         3615 g         4009 t
ORIGIN      
        1 cccgcccctg ctccagccgt tcccacgccc ccgccagcac ttctgtgatg attgccagtt
       61 gggtattggc ttttgccgcc agtttgtcga ttaacgggaa aaaggcaggt gtcggtacca
      121 gcgcttcggt cacaatggtg agatgcgttt cccaaccgcg cgcgagagct tccgcatcag
      181 tagtcagttt atctgcggct tccagcaaaa cgcgccctcg ctccagcaac atcctcccga
      241 cattggtgaa tttggtgcga tggcccgagc ggtcaaacag caccacatcc agctcttctt
      301 ccagtttttg catggtgtag ctaagtgcgg aaggcacgcg tcccagctca tccgctgccg
      361 ccgcaaaact gccccggcga tcgatcgcat ccataacccg tagtgcttcc agcgttaatg
      421 ccctttcttt ggccatttcg ttctcattca ggaaatttga acataccggg cagaatatct
      481 ggctaacaat gtagcgtcca gccccttacc ataaaaggaa gtaaagagag gtcaagaatt
      541 atgattacta cccgaactgc caggcagtgt ggacaagcag actacggatg gttgcaggcc
      601 cggtatactt tttcctttgg acactacttc gacccgaaat tgttaggcta tgcctccctg
      661 cgtgtgctta accaggaagt gctggcccca ggtgccgcct ttcagccgcg aacctatccc
      721 aaagtcgata ttttaaatgt gattctggat ggagaagcag agtatcgcga cagcgaaggc
      781 aatcatgttc aggccagcgc cggagaggcg ttgctgctct ctacccagcc gggtgtcagc
      841 tatagcgaac acaatctcag caaagacaaa ccgttaacgc gaatgcagct ttggctggat
      901 acctgcccgc agcgagagaa tccgctgatt caaaagctgg cgcttaatat ggacaagcag
      961 caattaatcg cctcgccaga gggtgcgatg ggaagcctgc aattacgcca acaagtgtgg
     1021 ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt tccagttgca tgggccacgc
     1081 gcgtatttgc aatcgattca cgggaaattt catgcgctta cgcatcatga agagaaagcg
     1141 gcgctaacct gcggtgatgg ggcgtttatt cgtgacgagg ctaacattac gctggttgcc
     1201 gattccccac tgcgcgcttt gctgatagat ttgcctgtct agttgttttt acagggagat
     1261 gatgatgagt aaaaaatcga ccaaaaagcg ccagccagcg aagcccgtgg tggcgaaaga
     1321 acctgctcgc accgccaaaa attttggcta tgaagagatg ttgagcgagc tggaagctat
     1381 cgtcgcggat gctgaaacgc gtttagccga ggatgaagct accgcgtaaa gacaggttgc
     1441 cggatgcggc gtaaacgcct tatccggcct acaaaacaca acaacatcaa caaattaaac
     1501 accccgtagg ccggataaga tgcgccagca tcgtatccgg catcaggtac aaaacacaac
     1561 aacatcaaca agttgagcac cccgtaggcc ggataaggtg cgccagcatc gcatccggca
     1621 caatactcaa aacctggcgt ttatctggcc ttgctcgcca taatctcgat aatctgccga
     1681 tccgtttgct gcatcgaatg gcttgctaac gcacacaggt tggcaatcga ctgctcaaca
     1741 tcatgcgcca cgatcccttc attgccggtc acggcggtat catccagcgc cattaacacc
     1801 gctttccacg cagccgaagc actggtcgaa accttcatcg cgcagctgtt cgacgcacca
     1861 tcgcaaatca tgccgctgac atcgccgatc atactgctga tcgccatcga gatagtttca
     1921 taacgcccat ccaccagcca tgccatcccg gcggcggccc ccattgctgc ggtcgttgcg
     1981 gcacaaagtg cagacaaacg cggtaactgg ttatggatgt aaattgcgct caaatgagaa
     2041 agcatcagcg cacgcgccag tcgttcatca tccgctccga agtgttctgc tacaaccacc
     2101 acaggcatta ttgcagtgat cccctggtta cccgagccgg agttactcat agccggaagc
     2161 gtagcgccgc ccatacgcgc atcggatgcc gcgctggtac gaatcacaat ggatgaagag
     2221 agatctttcg ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc aatatgcagc
     2281 ccccacttac cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc ggaatcgaga
     2341 ataaagcgga tcgccgcaaa tgggacttca ttgacgaact tcaggatctc agccagcgtc
     2401 gttctggaaa gcaccgtcag cggcgactct tgctcgccct ctgccacaca cgcctggtgg
     2461 gtaaacacca caccattgtg cgtctcaata tgcacaatgt tggtatgccc gccgacgatg
     2521 gtgacacacg cccacttctc accgttccag actttggcgc gtgagaagag gatttcattg
     2581 caaggttcct ggatcttaac agagactttc cccgccgcca gcagtgcttt ggcatcggca
     2641 attgcctgcg cagttgcgtc tttcagcact tccagcccgg cgttggcatt tccacctaac
     2701 gcccccagcg ccgccgcaat cggcagcccc accattcccg tgccgggaac ggtgacgccc
     2761 agaccgttct tcatcagatt tggcgaaacc caggcttcta cacgttcaac cggaccttcc
     2821 agttctgccg cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt acatcccagc
     2881 gccggtttta cttcctcctg aacggcgagg atgtaacgct gccataacgg atttaaagtc
     2941 gaatcaaaca tattaaaaac cttaaaattt caggtaaatc aagaaaatgc caggaacaga
     3001 gaaacacaaa gcaacaaacc agtgacgata atcaggtaca gagacatccc tttgtatttg
     3061 tgcaatgccg gtactttgta aaccagccac gccgggatca ggcaccctac catgccgaaa
     3121 atcgggctac agatagaggt gaagctcaac accggtgcgt tcagtacgat ggcgctccag
     3181 gccagcaaaa tggcgaaaat catgatgccg cgctgaacaa gattttcgtt aatcttctcg
     3241 gcaggcatct tgcgacgcag gatgttcatt acgatccctt gcgttgcttc gcgaaagcct
     3301 aaatagacgc caaagaatgc agtcatgaca gcaaagatat tgagaatgac gctgaccact
     3361 ttcacccacg ctgcgccgtc accgctaata aactgtgcgg caatcgccag cgcggaaata
     3421 ttctgctcgt aggcttttac cgcttcgtca tgtcccatcg ccagcgtgaa cgacacggcg
     3481 tagaaaaaga cggtgacaaa caaaatgcca aacgcgatat tcattgcccg caatgcttta
     3541 tgccgcgcca cttcaattga tttttcccgc gagcgataag agatcaccat cgggcttaac
     3601 gtctggataa acagaatcga cgtcagggta aacggcagcg taataatggc gtttttcacc
     3661 agcagcccca gcggcggtag cgaaccgacg ttatacagat gccacattcc caccatcgac
     3721 acgcccagcg cagcgactac cagcagcttg gtcagcacca tgtcggtcga aattttgaat
     3781 aacaatttct cgccgcgtga agagatcgcc accagaatgc aaatcagtac cagaccataa
     3841 aagggactgt ctgacagcaa cccttccgtc acgccgaagg tatgcagata ggaagcacta
     3901 tcgttggtga tggcggtgga atagacgaac atccaaatca ccagcattac gaaatagagc
     3961 gcacctaaca ggatgcccca gtttttacct aaataaccgc taatgacact cgggtaatct
     4021 ttacattctg gtgattctgc cagcgtatta ataaacaacc gctgaaacag atacattgcc
     4081 gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc gacctgcacc
     4141 gggagaaaaa caatccccgc gccaatcgcc atcccgatac tcataatcac ccagccggtg
     4201 tcagtactgt cgaatttgat cgcttctcgc cactcgctct cactcattcc agcacgaccc
     4261 gccggggtcg aagcgtctgc aatgacgcct ttattcgatg caatttccat aatttctcgc
     4321 tcaatatttt gtagggctta ttattttttt ccgagccgca tcaaggcgat atgcggtcta
     4381 cgtttttgca ggcgagttat tagaataaag aaatgatacg cgcgaggtca gagaaaatct
     4441 tcacaatcaa gactttaaaa taacggagat ggataagaat tttctactaa attaatcgca
     4501 agagaatatt cattctctat ttgcggcaga tcacaaaaaa aaggtgcaca tttgtgcacc
     4561 caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca gtcctccgcg
     4621 tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg tctcgatcac
     4681 tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg cggcgtttac
     4741 tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct gtccggcaac
     4801 cggatcgcag gtcagcccaa ggttatgctc catcgcgatt tccgccgcat tgcatacctg
     4861 cgccggactg ccgcccagta gttcagttaa ccctgccgct gccattgaac aggccacgcc
     4921 aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt tatacagcgc
     4981 gccaatagcc cccgctgcca gaaaatagcg ggcaattgac cgctcgttta ccggacgacg
     5041 gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt tagtcggtgc
     5101 cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt acatgttgat
     5161 ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctagaaacca gctgacgacg
     5221 cagcgctacg gcacgacacg gcacattgag cggaccaggc agcacgcctt cagtgttcat
     5281 cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaccctg cgtcaatttc
     5341 cgctttgctg cgcagcgcca gctcgttgtg catcatcagg ccagatatag acaggccgtt
     5401 gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta cggacgtttc
     5461 gacatcgtgc gacaggccga agtgttcttc ttcgacaata aacccgccgc cgacagagta
     5521 ataggtttta cttaatagcg cttcctgtcc cttccaggca gtgatccgca ttccgttctc
     5581 atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa aatcaacaat
     5641 atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta taaatgcagg
     5701 gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga taatggcgac
     5761 atccgtggca tggcctttgc ccgtcagtga caacgacccg tacagatcga ccacaatatg
     5821 gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt ttccggcatt
     5881 cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa tatcgaatgc
     5941 actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa cgaccttgcg
     6001 accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat ttccagcttc
     6061 acatctttcg gcaaacgcgc aacctgcaca cagctccggg tcgggtaggt cgcctgatgc
     6121 tcatcgaaga actgcttata gacttcattg atggtggcaa aatcattcag atcggtgatg
     6181 aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac gatcgctttg
     6241 acgttttcga ggcttaaacg tgcctgatct tgcacatcag ccgggatctc accggtctgc
     6301 gggcaaaccg gaatttgccc ggaggtgaag accatgctgc ctaaatcaac gccctgaaca
     6361 taagggccga ttgcgcctgg ggcacgttgc gtttcgataa tctttttcat acatcctccg
     6421 gcgtcagagc gcctgggtaa aagtacgtga aataacatcc tgttgctgtt cacgggtcag
     6481 tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat atttttccgg
     6541 gtgctcgatg gcatccagca gcatttcccg attcattacg ttgacgttga ggtgttgacc
     6601 gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccaacaa ggttggtttt
     6661 acgtactgga tcttctttgc ccagcgccgc aggaacgatt gagaaggtgt acgagatccc
     6721 atctttggcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca cggcaccttt
     6781 gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg cgcgacgacc
     6841 gtccggcgta ttaccggttt tctggccgta caccacgtta gaagtgatag tcagaatcga
     6901 ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca taaagcgttc
     6961 aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact gcggatattc
     7021 accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca cggattggtt tcacgcgggc
     7081 atatttgatg gcagacaggg agtccgtcgc caccgacagg cccgcgatgc cgcatgccat
     7141 agtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc tgtacttgtc
     7201 gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc accgccagcc agtccatgaa
     7261 gtgatcgagg ctgtccatca ctttgtcgta atccagcacg tcgtccatca gcggtgctgt
     7321 tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg cgtagagcag
     7381 cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa tcaccattgg
     7441 gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca tcagatcgtc
     7501 attttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt ttttgaaggc
     7561 aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgccg gccccatagt
     7621 gtgcagggtg tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac cgtccagccc
     7681 catcccgccg atcacttccg tcgcccagat tgggtcgccg gagaacagcg aatcaaattc
     7741 cggtgtacgc aggaagcgta ccatacggat cttcatgatg aagtgatcga tcagttcctg
     7801 tgcctgctgc tcattgagga cgccagcttt aaagtcgcgc tcaatgtaga tatccaggaa
     7861 cgatgccgtg cgccccagcg acatcgcgcc gccattttgc gatttcactg ccgccagata
     7921 agcgaagtag agccactgca ccgcttcctg tgcattctgc gccgggcgag agatatcaaa
     7981 gccatatttc gccgccattt cctgaatctg caacagcgca tgacgatgct ctgccagctc
     8041 ctcacgcaga cggatggtgg cttccagatc ctcgcctttt tccagacgag actggagatc
     8101 ggcaaattgc agttcgcgtt cacgcaccag ataactgatg ccatacagcg ctacgcggcg
     8161 atagtcaccg ataatgcgcc cacgaccata gccatctggt aaaccggtca gcacgccaga
     8221 tttacggcag cgcagcatat ccggtgagta aacatcaaat acgccctggt tatgggtttt
     8281 acgcagatcg gtaaacagat attcaaattc actgtccatt tcacggccat aggcgtggaa
     8341 tgaactttta atcatattaa tgccaccgaa cgggtgtagc gcacgtttca acggcgcatc
     8401 cgtttgcagg ccaacaattt tttccagcgg ctggttaata tatcccgcat catgagcggt
     8461 aattgtggtg gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt tttcgatacg
     8521 gatgccttcc attacctttt cccacaattc cgtggtggca ggcgtcgctt cggcgagaaa
     8581 agattcatcg ccttcatacg gtgtatagtt atgttgaata aaatcgcgga cattaatttc
     8641 gcttttccag tccgtacctt taaagccaag ccatgcgtca gcgtacagct tatcgctggt
     8701 atcaatatct accttcatga aaaataatct ctctacaata cttcaactaa attatgcaaa
     8761 ttctgcgggc gcgttaactt tacctaaatg aatggcatcc aaagcaatca ttttttcttc
     8821 gttagtcgga ataacgacgc aaatgacatg cgcattttca ctggaaacaa ttcgctcgcc
     8881 aaaagagtta gatcgattat tcatttctgt atcaatctct acgcctaata cagccaaatg
     8941 ttccatgacc agacgacgaa ttaaacttga attctctcct attccgccgg tgaatataat
     9001 tccatccagc cgacgtaatg aagctgcatg tccggcaata tgacgggcaa ttcggtgaac
     9061 aaaggtttta attgccagtt gcgcgcgttc gtgaccttca tgccaggctt tttccagaac
     9121 acgtaaatcc gaagaaagac cggaaatacc taataatccc gactctttat tcactacgcg
     9181 ttccaggtca cccaggctct ggttggtttg gctggcgacc caggacatcg caccaaagtc
     9241 gacatcgcca ctgcgggtac ccatcatcaa gccttccagc ggcgtcattc ccattgaggt
     9301 gtcaacactc tgaccgttgc gaactgcgca gattgacgcg ccattgccaa gatgcgccac
     9361 aaccaggccg gagtcatctt ccgccagatt cagcagcgaa tgtgcgcgct gggaaacata
     9421 gcggtgcgac gtgccgtgga aaccataacg gcgtacacct aactcttcat aatatttcca
     9481 cggcaggccg tataaataag cttccggagc catcgtctgg tggaaactgg tatcaaatac
     9541 cgccacctga gttacgcccg gaaataattg ctgcgccgat tcaataccac ttaaatttgc
     9601 gtaattatgc aggggtgcca gtggagaaac gcgacggata ttatcaatga cttcatcggt
     9661 aataatggcg gactcggtaa aaatactgcc gccgtgagcg atgcggtggc caattaaggc
     9721 cacgctgtca tttaaattcc gtttttccag ttcaaatgca attgccttca atgcaccttc
     9781 gtagctgtgg tgagccagcg gtgctggctc tcccccattt acggataaga atgcattttc
     9841 cgagttaata ccgtcggcaa tacctgacat taatacttca caatcgctgg catcgagtac
     9901 ggaaaactta atcgaagacg aaccacagtt aataaccaaa acaaccggaa attcattcat
     9961 ctcttttctc atcctgagtt acggattaaa acagtttgta tacgatgttc aggatggtca
    10021 gcagaccaat cacggtaaca aacacgttat ccagacgacc acggtatttc gccagagacg
    10081 gcgctttacg gatagcatac atcggcaaca ggcacagcag ggatgcgata atcggtgcgc
    10141 ccatggcttc aatcaggtcg aggatgttcg ggttggcgta ggcaacaacc caggtggagc
    10201 ccatgatgaa gatcatgctg agagtattca gtttacccag cgacactttg gttttgtcac
    10261 ctttataacc gaacttcaga atcagaccat tcaagccttc cagcgtcccc agatagtgac
    10321 cgaagaaaga tttgaagata gccacgagtg cgatgatgga agccgcatat tccagtgtaa
    10381 tcgcgaacgt tgttttcgta ccggtcatgg acgcaaagtg gttagccaga taagaaagca
    10441 ctggaatatt ctgcgctttg gcttccgcca tgttggccgg agacagagta aacaggcagc
    10501 taaaggcaaa gaacatcacc actgcaacca tcagcatgct ggcacgagaa atgatttggg
    10561 aacatttacg ttcggtgaag tcgcgaccga agtctttctc atactcttca cgtttagaaa
    10621 ccacgaagga agagacgatt ggcgagaagt taaaggagaa aaccatgatg gaaatcccca
    10681 gccagacagt gatcaggata ccgtcatgac cggttaacga cagcgaaccg aggtcaacct
    10741 ggtcgataac tgcagagttc cagtaaggga tcagcgacag agaaatcagc accaggctgg
    10801 cgataaacgg ccataccagg tagctcatca ctttaaccat cagatcctta ccaaaccaga
    10861 tgacgaaagc catcagcagc aacaggaaca gcgccacaaa gccgcgattc agcggtgcaa
    10921 atccgagctg gttttcccag aacgtcataa aggtattggt aatagtaacg ccataaatcc
    10981 acagcagtgg gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg ccagttttac
    11041 caaaatgctc ttccaccgtt tccgtaatgt tgccggaagg gttagagcca gaaagacaca
    11101 gacgcgccag cgcccggtgg caataaaatg cgatggggta tgccaatacc aacatcagaa
    11161 gaatcgggat cagtccgcca aaacctgcgc ggatagggaa gaacagcacc ccggcgccga
    11221 ttgccgtacc aaacaagcct aacgtccatg tggtatctga tttacgccag gacgattgtt
    11281 ttgtctggct ggatacaatg ctatctgaag tactcatatc ctatcctcaa cgaattaatt
    11341 aagcgtcaac gaaaccggtg atttgagaga cgcgagaaag atcgatattg ccgccggaaa
    11401 taatactgac ggtttttctg ttttgaatat attggtctaa tttaccgctt aataatgcag
    11461 cacatgccag agcgcctgcg ccttcggtga cgactttatt gcgctgaatt aaggcaatca
    11521 tactgtttct gatttcgtct tcgctgacca gcacgatgtc atcgactaat tcacgaacga
    11581 tttcgtaagt taaattaccc gggcgggaga catcacaacc atccgccagg gtgccggtag
    11641 ttcggtgcgt ggttatttct ccggagtgga aagaagccgc catgccgtga acgttttctg
    11701 actgtacgcc aataacacga atggtcgggt taatagattt aattgccacc gcaataccag
    11761 caattaaacc accaccacca attggcacaa tcacgttatc gacatcatag agatcttcca
    11821 taatttccag accaatcgtt ccctggccag caatcacttt cggatcatcg taaggtggga
    11881 taaaaatacg accttccatt tcgacaattt cgctcacttt agcgatagtg tcgttgaagt
    11941 tatcaccatg cagaacgact tctgcggagt agtcgcacgt tgccgctact ttggattttg
    12001 gcgcaccttt tggcatcacc actttaccgt cgatacccag catcgcgcag gagagcgaaa
    12061 ccccttgcgc atggttgccc gcagaacagg ccaccacgcc tttgcgtttt tccgcatcgg
    12121 tcagtgaact taatttatta aatgcgccac gaattttaaa tgaacccgta cgctgcatat
    12181 tttcaaactt caggaatatt tcacctttgc aacgttcact aaaatagttg gagcgaggca
    12241 tgcctgtttt ataaattcgc ccagccagtc gttgtttcgc ttcaataata tcatcaatag
    12301 caaccggcag atcgtatgta atatgcatta taaaacctct tcgcctgaat taaatatgta
    12361 ggtaaccgta accgacacct gcaaaacaga caggtggatt atttatgttt aaataaaaac
    12421 aaacaactaa ccaacttcta ttaattgcct tcgtctacac ccattataag atgaatactc
    12481 tttggctaat tccaccaaaa ccgatgctgc ttttttaata cgataatttt tcgaccatac
    12541 cgcggcatat tgtgccacag gtaatgtttc ttcaaccgga atagtaataa attgattaga
    12601 accaaaaggt gacgtcatat cacaaggaat tacagttaag aaatcagcat tgagaacaag
    12661 attataaatt gtcacgactg agtcggtttt aacgatgttt tcaatactga tgccatttct
    12721 ttgtaacgta gtaagcagtt cgctgtagta ccccatatta gtttgtggca acacccactg
    12781 ttcgttcttc aacgactcca gcgtggtggt gccggtgcat gttcgggact tactggctac
    12841 cagcacaaac tcggactcga acagcggctc aacatgtaaa tcctgaagct tcatttctgc
    12901 acttaacgta ccaatcgcaa aatccagtcg accgtcgcgg attgccggta agaacgaaga
    12961 cagttgcgct tcatacatag aaacctgcgc tttcgggaac acctctttga acttgttgat
    13021 catccctgac ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga cttccaccac
    13081 cgcttcagaa gacataccgc ttatctcatt aaccatattt ttcatttcac gggtaatgga
    13141 ttcggaacgg gagagtaaca attgaccggc aggtgttaat gttacaccgg tatttttccg
    13201 caccactaat tccacaccaa aataatcttc aatatcgtta atgattttac tgacggccgg
    13261 ttgagttaac cctaattctt ttgcagccga gccgatagag ccacttctaa tgacttcctg
    13321 aaagactacc aggtgctgcg ttttcggaag aagaatagtg ctcataacga cctacgttaa
    13381 ttacctcatt gacggcatga agtgtatcaa aatgaaataa acaggatatg tgcgaccact
    13441 cacaaattaa ctttcattac tttccaaggt atcgttatta caattaaata tctttaaata
    13501 tcaacaagtt aaagcataaa aatcagcata aaccctgatt tttatcaaaa aaaaatgacg
    13561 gggataacca tattttatgg cgataacatc attcgttatg tggttaatac aaaaaagggg
    13621 ctgagaccat atttcaagca attaccggat aattaccagg caaaatttga gttaaattta
    13681 atgagcaagt tctcaaattt tattataaat aaaccatttt cagcgataaa tactgcggca
    13741 cgtcacattt tttcacgcta tttgttggag aacaaacatt tattttatca atattttaaa
    13801 atttcgaata caggtattga tcatctcgaa caattgatta acgtcaactt tttctcttct
    13861 gacaggacgt cattttgtga atgcaatcgt tttccataaa ttcttctccc ctcataggcg
    13921 acgaatagca ttttgtgttg aggatcacaa aacgaataat tgctggtcgc cgcgataagg
    13981 tcagacgaag tcaacaaggg ggattttcac tgtgctttac atcagcgtag gacacagaat
    14041 gttgcttcgt ttactgtaac gccgggtaaa tgagcgtttt ttgatagtgc gtaagtgtcg
    14101 tgcgaggaaa ataagcatct attattgttt gtcatgacga caaggcaggc atcaatgaaa
    14161 ggcttcccaa ttgcacatat ttttcaccct tcaatcccgc caatgcacgc agtggttaac
    14221 aatcacaata gaaatattga ttattggacg gtaaaaagaa agtttgcaga aattgtctcc
    14281 accaatgacg ttaataaaat ttacagtata agtaatgaac tccggagagt attatctgca
    14341 attactgcat tgaatttcta tcaaggcgat gttccttctg tcatgatccg aatccaaccg
    14401 gaaaatatga gtccattcat catagatatt tctacaggag aacatgatga ttatatcata
    14461 caaacattag atgtaggcac ttttgcacct tttggtgaac aatgtacttg ctcagccgtc
    14521 aataaaaaag agctggaatg tattaaggag acgatttcta agtattgcgc aaagttcacc
    14581 caaaaagaag ccatcttaac accccctagg cactttaata aaacctgcat tacttcagat
    14641 tgttggcaaa ttctcttttt ttcacccgat cattttaata atgattttta ttgattattt
    14701 ataaaggata aaataatgtt ccctgtttca tcaattggta atgatattag tagtgattta
    14761 gtccgtcgaa aaatgaatga ccttcctgaa agccccatag gaaataacct cgaagcacta
    14821 gcaccaggta tagaaaaact aaaacagacc tctattcaaa tggtcacttt acttaacgcg
    14881 ttacaaccaa gtggaaaatg cattatcact ggtgattttc aaaaagaatt agcgtactta
    14941 caaaatgtaa ttctttataa tgactcgtct cttcgtctgg attttttagg ttataacacc
    15001 caaattattc aacgatcgga caatacctgc gaacttacca ttaatgaacc gttaaaaaac
    15061 taggaaatat ctacaggcaa tatcaatgtt aattgcccat taaaagatat ttacaatgaa
    15121 atcaggaggt tgaacgtaat ctttagttgt gggactggag atatagttga tctatcctct
    15181 ctggacttac gtaatgtcga tttagattat tatgatttca cagataaaca tatggctaat
    15241 actattctaa atcattttaa attggattca acaaatttta ctaatgccaa catgtttcag
    15301 gttaattttg ttagttcaac acaaaacgcc acaatctcct gggattattt actaaaaata
    15361 acgcctgttt taataagcat tagcgatatg tattctgaag aaaaaatcaa gtttgtcgaa
    15421 agttgtttaa atgagcttgg agacattacc gaagaacaat taaaaattat gagatttgca
    15481 attataaaat ctataccaag ggcaactctt acagataaat tagaaaatga attaacaaaa
    15541 gaaatatata aaagctcatc gaaaatcaac aattgcttga acagaattaa attaccagag
    15601 atgaaagaat tctcatcaaa aaaaatatat gattacatcg atacaatcat tgaagattat
    15661 gaaaatatca aagaaaatgc ttatctggtc gtcccccaaa ttaattatac tatggattta
    15721 aacatagaag actctagctc agaagagtta ataccctcga gaaagacgaa aattctccgg
    15781 acaatggctt tgaggtcggg gaatataaca catatgaagc atataactca gagaatcaat
    15841 attttaccag agaggactat acgtataatt acgacctttt aaatgcaata tagacttaat
    15901 aacttcttac aaagtctatt attaataccg tagatattca tcttaccctg tgcaccaaaa
    15961 gcaaaaactt atcgacgcgg gtttgaaact gatggcaacc gcaaaaatgc ctgatgcgct
    16021 acgcttatca ggcctacgcc atctctgcaa tatattgaat ttgcgtgctt ttgtaggcag
    16081 gataaggcgt tcacgcagca tccggcatga ataaagcgca ctcaacaatc tgaaacccgc
    16141 cggaacggtt tattacgtac atcaggtgaa actgaccgat aagccgcttt cttttgggta
    16201 tagtgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc cgccgaagcg
    16261 gg
//
LOCUS       DAJTUN010000092        16231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTUN010000092 DAJTUN010000000
VERSION     DAJTUN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.092"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..69)
                     /locus_tag="OO470_002794"
     CDS             complement(<1..69)
                     /locus_tag="OO470_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR6251990.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVY"
     gene            complement(174..410)
                     /locus_tag="OO470_002795"
     CDS             complement(174..410)
                     /locus_tag="OO470_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6251991.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(528..1967)
                     /gene="rsmF"
                     /locus_tag="OO470_002796"
     CDS             complement(528..1967)
                     /gene="rsmF"
                     /locus_tag="OO470_002796"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6251992.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2047..4667)
                     /gene="yebT"
                     /locus_tag="OO470_002797"
                     /pseudo
     CDS             complement(2047..4667)
                     /gene="yebT"
                     /locus_tag="OO470_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4636..5919)
                     /gene="yebS"
                     /locus_tag="OO470_002798"
     CDS             complement(4636..5919)
                     /gene="yebS"
                     /locus_tag="OO470_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6251993.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6049..6546
                     /gene="msrC"
                     /locus_tag="OO470_002799"
     CDS             6049..6546
                     /gene="msrC"
                     /locus_tag="OO470_002799"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6251994.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6643..7341
                     /gene="proQ"
                     /locus_tag="OO470_002800"
     CDS             6643..7341
                     /gene="proQ"
                     /locus_tag="OO470_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6251995.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7361..9409
                     /gene="prc"
                     /locus_tag="OO470_002801"
     CDS             7361..9409
                     /gene="prc"
                     /locus_tag="OO470_002801"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6251996.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9601..10482
                     /gene="htpX"
                     /locus_tag="OO470_002802"
     CDS             9601..10482
                     /gene="htpX"
                     /locus_tag="OO470_002802"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6251997.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10528..11901)
                     /locus_tag="OO470_002803"
     CDS             complement(10528..11901)
                     /locus_tag="OO470_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6251998.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12078..12869
                     /gene="kdgR"
                     /locus_tag="OO470_002804"
     CDS             12078..12869
                     /gene="kdgR"
                     /locus_tag="OO470_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6251999.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13012..13251)
                     /locus_tag="OO470_002805"
     CDS             complement(13012..13251)
                     /locus_tag="OO470_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252000.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13410..13553
                     /gene="mgrB"
                     /locus_tag="OO470_002806"
     CDS             13410..13553
                     /gene="mgrB"
                     /locus_tag="OO470_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6252001.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13628..13915
                     /locus_tag="OO470_002807"
     CDS             13628..13915
                     /locus_tag="OO470_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6252002.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14494..14637
                     /locus_tag="OO470_002808"
     CDS             14494..14637
                     /locus_tag="OO470_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6252003.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14650..14859
                     /gene="cspE"
                     /locus_tag="OO470_002809"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14650..14859
                     /gene="cspE"
                     /locus_tag="OO470_002809"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6252004.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15025..15834
                     /gene="rlmA"
                     /locus_tag="OO470_002810"
     CDS             15025..15834
                     /gene="rlmA"
                     /locus_tag="OO470_002810"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6252005.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15831..>16231)
                     /gene="mntP"
                     /locus_tag="OO470_002811"
     CDS             complement(15831..>16231)
                     /gene="mntP"
                     /locus_tag="OO470_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6252006.1"
                     /translation="MGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPR
                     RRHGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFI
                     GSIIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4247 a         3954 c         4132 g         3898 t
ORIGIN      
        1 gtagacatcc acaacaatat cttcacaacc gccatctagg tagcaaacaa aaagtaccag
       61 cgcgaacatt tcatccccga agtgtggtgc cgtaccgtta agtttaggag agattttaca
      121 acgggggaat aaccaggaca aataacccgc catgccgacg ggttcttttt ggatcaggca
      181 agacgcataa tccaggcatc agattgttga tcgtaacggg tacggtaaag gacagagtgt
      241 tcgccattta gcagggcagg gatactggtt tcagcatccc aggcatccag ttgcatacat
      301 aaatcggggt cagatttaca aggaatggtt aacgttcgat cacccggcga ttcaccgtgt
      361 aattcgccgt cgtcgatttc aaaagcgcct atgcgcacac tggttttcat cttcgtgctc
      421 tccatttagc tgctgattgt agtacgacca gtccgatcgc acaatttttc agcctagttg
      481 aggccggcga atgtgccagt caaaaagtgc gctttttttg tgcgctgtca tgcgttactg
      541 gtaaaaagtt tcccgtcccg caccagttca cgcgggtagc tgtttttcag tcgcgaacca
      601 atccgtttgg ctaaaccaat cggctgatgc tggaaagtaa ccaacacgtc atccgccact
      661 ggcgcggctt gcgggtaaac atcgcgcccg cgataccact cctccgcttc ctgcggtgtc
      721 agttcaaaag cgtttacatt gtcgggggag gcatgggcaa taaccgcttc atgctgccag
      781 cgataacctt tgttatgcgt ttcggcaagt ttaatcccta agcgagaaaa tcggacttta
      841 ccgatcaggg cttcaatgcc taccgggaac aaccacagtt ctttgtcgcg ttgccagagt
      901 cgcaggtttc catcccagtt taagccaaca cttgcagccg cctgacgaat ttgtccggct
      961 tcgcgatctt tcaccgggct gaacgggaaa ttgcccactt tgtattttgg ggcgggtaag
     1021 gcggggatcg cctgagtttt acgcagacga gcaacgaaga agccttcgca gtcgtaaatt
     1081 tgtgggaaaa catgcaaaaa accttcttcg gtcagcgcct tgtttgcacc agggaagaga
     1141 tcgccaagtg gtaaaaactc tacttcgtcg gggtaagtct ctttcagcca ccggcaaacg
     1201 gcttcgtttt cttcccggtt taaggtacag gttgagtaaa ccagcgtacc gccaggacgt
     1261 aatgcatgaa aggcgctgtc aatcagttcc cgttgggtag ctgcgatttc ctgattgctt
     1321 tcgggtgacc agttttttag cgcatcggga tctttacgca ccacgccttc gccggagcag
     1381 ggagcgtcca gcaaaatggc atcaaacatt tctggcactg ccgcaccaaa cacgcggcca
     1441 ccaaaatgtg tgagcgcaac attactgatg ccacagcggc tgatattggc atgtaacact
     1501 tttacccaac tggcggaaaa ctcattggca aggattgccc cttcgttatt catccgcgcg
     1561 gcaatttgcg tcgtttttga gcctggcgca gcagcgacat ccatcacccg ctgtggtgca
     1621 ttaccgtcag caaacaaggc ggcgacgggc aacattgaac tggcttcctg aatataaaac
     1681 aggccactta aatgctcggt ggtactaccc aatggcaatg catcttcatt gtcgcgttca
     1741 atccagaaac cttcttcaca ccacggaatt ggcgtaagcg tccagccata aggagcggtt
     1801 aattgcagga aatcagcaac ggagattttc agcgtattaa cgcgaatgct gcggcgcaac
     1861 gggcgctgac aggcggcaag aaaatcatca aatgagagcg tcgaaggcat agcttcgcgc
     1921 atttgtgtca gaaacgcgtc cgggaaataa acggtgtgtt gggccacgag catgtaccac
     1981 tggcaaaaaa acaggcgcgc agtttagcat aagcgctccg gcgcaggcac gccggagcag
     2041 tgggcattat ttgggaagcg cagttcccca ttcacgccac tctttcggtt cactttcctg
     2101 caacaggaag tgtttgcctt cttgggcttt cggtgcaagt ggcgtacccg gaggcgtggc
     2161 gaaggcgatg ccgccacgga taaattggtt aaaggtgccg gttttcacta cgccgcccgt
     2221 cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt tacgcaccag
     2281 gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca atgtccccag
     2341 cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg taccgatgcc
     2401 taacgaaccg gcttccggcg cttcaacaat aatgcttaaa ccatctaggt aacgcgaatc
     2461 agtaatggtg gcctcttcta attcaaagtc gcgacgagga ttgccacggc ctggttcgac
     2521 gttgatatac ggctggagga tagtatctag atgctcaacg ccagctgccg agatttgcgg
     2581 tgtgaccact gagaagcgcg taccaccgcg agcaaaggtc tggacatatt ccggatagag
     2641 caccgccttt gcctgtactt cattgcgtgc ggtaatcaga tccagcgtct ggatttgccc
     2701 gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg catcgaaagc
     2761 gtgaagcgta atctgcctgc caaccgcacg ggccgctgtt tcggaagcat acagaattcg
     2821 cttgtcgcct ttacgctgac tggcgctggc accactgagg ttatcgaagc taatggcccc
     2881 ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat tcagctgaac
     2941 tttcgccccg ccttctgccc agaacacgct gttgctggtc aaaaggttgc gatattccgg
     3001 tttaatgtgc agatcgatat cgaacgcgtt agctcgcgga cgcacggtaa taacttcacc
     3061 aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca gcgtctttgc
     3121 actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg ctttttccag
     3181 attggcatac agtggatagc tggctttcat ctcgccttta tcgcccggca gaatacgtat
     3241 cccgccgtta atccattctg aggcgctggc accgagaaac tcaacgccat ccagccccac
     3301 cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca gttctcgatg
     3361 ctgaggctcg atggcgacgg taaaggtgac gcctttgctg gtgagtttac gatcgataac
     3421 ctggcctacc tgcacgccgt gaagaatgag cggctgaccc gcatcaatac cgtaactttc
     3481 cggtgctgtc agggtcagtg tcagaacgtc cggctcttgc agcagggctt tttcgcccgg
     3541 cacaacaacg aactctttgc gtggctcgcc atcgccgggc accaactcaa aggtttttcc
     3601 ggtcagcagg gcgctgagat tagcatcgct aagggataat ttcgggttgc gtaattcgat
     3661 gcgggtattt tcacgaagca gggatcaacg gtcatttccc cagtgacttt accaccagga
     3721 tttaaatcca gtttagtcag ctgtccgact tccagcccct gatacattaa cggcgtcgag
     3781 tcggcggtta atcctgctcc actcggcagt tccagtttta ttattactcc acgctggctg
     3841 tgggccagat cttcatacag accaaaggta tcttccgcct cggcaggttt cgactcttca
     3901 ggtgaatcga aggcaatcgc accgttaacc agtgccgcca gactttccag tttcaccttc
     3961 gcgccactga tactgacgtt ggcatcaacg ccggaaacgt ttcagaaacg gctacctttt
     4021 ttcaccagat cggtaaaacg ccgctcgatc aggacatcaa tcaccacgcc ttgcttgttg
     4081 ggattgatgg catagtcgta gacttttccc accgggatct tgcggaaata gaccaatgaa
     4141 ccgctgttca gcgaaccgag agcgggggct tgcaggtgga tcatcagatc gccattgtcc
     4201 agccgatatt tcggttgggt atcgagcgcg acaaagtgat cctgctcttt acctttaccc
     4261 ggcatcatgc cgatatagtt cccaccgacg agggcgtcca gcccggagac acctgccaac
     4321 gatgcttttg gcgtcatcag ccagaactga gtctcttcgc gcagcgcatc tttcatatcg
     4381 gacttgatgc tgaccttgac ttcaatctta cgaagatcgt cgctgaggct gatatcctgc
     4441 actgttccga cttcaacgcc ctgataacga acaggcgtac ggcccggaac aataccatcc
     4501 gccgacataa agtcgatggt gacggtatta ccccggtcct gataactgtc ccaaatcagc
     4561 caaccggcaa tcattagcgc gatgaaaggc agcagccaga aaggtgagat acggcgttta
     4621 tttttaatct gcgcttcagt cgtcgaagcg ggcgtttcct gactcatgtg catcccaaag
     4681 taagcggctg tccagccatt ccacagcaag aatagtcaaa attacggcag cgccaaaata
     4741 aaacgcagcc ggtcccatag taaaagcgag gatctgatcg cgattaatca gcgacatggt
     4801 taaagatatg acaaacaggt cgagcatcga ccagcgtcca atccaggtca ccatccgcag
     4861 taacagaatg cgtgtgcgta aaccttgctg gcatttaaaa tgaatgctca acagtaaagt
     4921 gaacatgacg atcactttag taaacggtac cagaatactg gcgataaaca cgattcccgc
     4981 aaccgcaatg ttgctacttg ccagcgacat aattccggaa agaatcgtat cttcctgccg
     5041 gccaccattc agataaatga tagaaatagg caataggttg gctggtaaca acaaaacgat
     5101 tgacgctaac agcgccgccc agcatttttg caaactatga cgacgacgca ggcgtagcgg
     5161 gatatggcaa cgcgggcaga gaccacgctg atcaggatag ccggtaaaat ggcacccaag
     5221 acagacacga agtttctcgt ccctacgcgt agcggggcgc tgcggataat atcgctccca
     5281 cagctcctca acattaagat gtgacaacgt caccgtcgtt aaaatcacca acgccacaaa
     5341 agagaacaag ccgacacccg cctggatatg ggcataatcc tgtaccttta tagaagcaac
     5401 gccaatgccg accaggtaga tatccagcat tacccactct ttaagtcgct caagcatcag
     5461 cagtaccgga cgtaaattca ttcccagtcg gttaccaaac cataaatatg ctatggagga
     5521 caccagaatg aggggggcac caataacgca gaaaaagacc attgatcccg ttatcgcatc
     5581 gccctgtttg gtcatttgcc agatgccttg catgacgttg gcgtcaatac gaatgcctaa
     5641 cagccagata tgcaacagcg gttcgcccca ggcaaacggc ataagcaaca gcatggtgaa
     5701 ggccattgcc gccaggcgcg ttagcgacca gtcgcgcccg tcgcgaattt ttgcctgaca
     5761 gcgcggacaa taggcacttt gatgagaatt tatctcgggc aggctaaaca gcatgtcaca
     5821 ttgcgggcaa cgttggtaat caccacgcgg cagttcctcg ccgattgccc tcactgttat
     5881 cttttttgtc ggcgtaattt gtggtgtgtt aagagccatg acgtgccaaa tgtaataaga
     5941 attaacatta atcttaaccc atgatgggaa tgatcttgag cattaacgca tttaatgctt
     6001 attttaatag gctggcagtt ccaggtaaga caaccaagtg attaagtaat gaacaaaaca
     6061 gaattttacg cggatttaaa tcgcgacttt aacgcgctga tggcgggaga aaccagtttt
     6121 ctggcaacgc ttgcgaacac cagtgcgttg ttatacgagc gtctcactga cgttaactgg
     6181 gcaggttttt atttgcttga ggacgataca ctggtactcg gaccatttca gggcaaaatt
     6241 gcctgtgtcc ggatacccgt cgggcgcggg gtgtgcggca cagcggttgc ccgcaatcaa
     6301 gtgcagcgta tcgaggatgt tcacgcgttt gacgggcata ttgcctgtga tgcggcgagt
     6361 aattctgaaa ttgttctgcc gctggtggtg aaaaatcaga ttattggtgt tctcgacatc
     6421 gatagtaccg tcttcgctcg ctttacagac gaggacgagc aaggcttacg tcagcttgtg
     6481 gcacagcttg aaaaagtgct tgcaacgacg gattacaaaa aattctttgc gagcgtcgca
     6541 ggataatcaa cggataacgt agcatttact ggtagcgtca ttataatgac gcctgttcat
     6601 gcctgcgctt gttggctacg tccgttgtaa tcaggaaatt tcatggaaaa tcaacctaag
     6661 ttgaatagca gtaaagaagt aatcgcgttt ctggccgaac gttttcccca ctgtttcagt
     6721 gcggaaggtg aagcgcgtcc gctgaaaatt ggtatttttc aggatttggt cgatcgtgtt
     6781 gccggggaaa tgaacctgag caaaacgcaa ttacgatccg ctttacgtct ctacacttcg
     6841 agctggcgtt atctttacgg tgttaaaccc ggcgcaacgc gtgtcgatct tgacggcaac
     6901 ccatgcggtg agctggacga gcaacatgta gagcatgctc gcaagcagct tgaagaagcg
     6961 aaagcgcgtg ttcaggcaca gcgtgctgaa cagcaagcga aaaaacgcga agctgccgca
     7021 gctgctggtg agaaagaaga cgcaccgcgc cgcgaacgca agccacgtcc gaccacgcca
     7081 cgccgcaaag aaggcgctga acgtaaacct cgtgcgcaaa agccggtaga gaaagcgcca
     7141 aaaacagtaa aagcacctcg cgaagaacag cacaccccgg tttctgacat ttcagctctg
     7201 actgtcggac aagccctgaa ggtgaaagcg ggtcaaaacg cgatggatgc caccgtatta
     7261 gaaatcacca aagacggcgt ccgcgtccag ctgaattcgg gtatgtcttt gattgtgcgc
     7321 gcagaacacc tggtgttctg aaacggaggc cgggccaggc atgaacatgt tttttaggct
     7381 taccgcgtta gctggcctgc ttgcaatagc aggccagacc ttcgctgtag aagatatcac
     7441 gcgtgctgat caaattccgg tattaaagga agagacgcag catgcgacgg taagtgagcg
     7501 cgtaacgtcg cgcttcaccc gttctcatta tcgccagttc gacctcgatc aggcattttc
     7561 ggccaaaatc tttgaccgct acctgaatct gctcgattac agccacaatg tgctgctggc
     7621 aagcgatgtt gaacagttcg cgaaaaagaa aaccgagtta ggcgatgaac tgcgttcagg
     7681 caaactcgac gttttctacg atctctacaa tctggcgcaa aagcgccgtt ttgaacgtta
     7741 ccagtacgct ttgtcggtac tggaaaagcc gatggatttc accggcaacg acacttataa
     7801 ccttgaccgc agcaaagcgc cctggccgaa aaacgaggct gagttgaacg cgcagtggga
     7861 cagtaaagtc aaattcgacg agttaagcct gaagctgaca ggaaaaacgg ataaagaaat
     7921 tcgtgaaacc ctgacacgcc gctacaaatt tgccattcgt cgtctggcgc aaaccaacag
     7981 cgaagatgtt ttctcgctgg caatgacggc gtttgcgcgt gaaatcgatc cgcataccaa
     8041 ctatctttcc ccgcgtaata ccgaacagtt caacactgaa atgagtttgt cactggaagg
     8101 tattggcgca gtgctgcaaa tggatgatga ctacaccgtt atcaattcga tggtggcagg
     8161 tggtccggca gcgaagagta aagctatcag cgttggtgac aaaattgtcg gtgttggtca
     8221 aacaggcaag ccgatggttg atgtgattgg ctggcgtctt gatgatgtgg ttgccttaat
     8281 taaagggccg aagggcagta aagttcgtct ggaaatttta cctgctggta aagggaccaa
     8341 gacccgtact gtaacgttga cccgtgaacg tattcgtctc gaagaccgcg cggttaaaat
     8401 gtcggtgaag accgtcggta aagagaaagt cggcgtgctg gatattccgg gcttctatgt
     8461 gggtttgaca gacgatgtca aagttcaact gcagaaactg gaaaaacaga atgtcagcag
     8521 tgtgatcatc gacctgcgta gcaatggcgg tggggcgttg accgaagcgg tatcgctctc
     8581 aggtctgttt attcctgcgg gtcccattgt tcaggtccgc gataacaacg gcaaggttcg
     8641 tgaagacagc gataccgacg ggcaggtgtt ctataaaggc ccgctggtgg tactggttga
     8701 ccgtttcagt gcttcggctt cagaaatctt tgccgcggca atgcaggatt acggtcgtgc
     8761 gctggttgtg ggtgaaccga cgtttggtaa aggcaccgtt cagcaatacc gttcattgaa
     8821 ccgtatttac gatcagatgt tacgtcctga atggccagcg ctgggttctg tgcagtacac
     8881 gatccagaaa ttctatcgcg ttaacggcgg cagtacgcaa cgtaaaggcg taacaccaga
     8941 catcatcatg ccgacgggta atgaagaaac ggaaacgggt gagaaattcg aagataacgc
     9001 gctgccgtgg gatagcatcg atgccgcgac ttatgtgaaa tcaggagatt taactgcctt
     9061 tgaaccggag ctgctgaagg aacataatgc gcgtatcgcg aaagatcctg agttccagaa
     9121 catcatgaag gatatcgcgc gcttcaacgc tatgaaggac aagcgcaata tcgtttctct
     9181 gaactacgct gtgcgtgaga aagagaataa tgaagatgat gcgacgcgtc tggcgcgttt
     9241 gaacgaacgc tttaaacgag aaggtaaacc ggagttgaag aagctggatg atctatcgaa
     9301 agattaccag gagccggatc cttatctgga tgagacggtg aatatcgcac tcgatctggc
     9361 gaagcttgaa aaagccagac ccgcggaaca acccgctccc gtcaagtaat atcaatcagg
     9421 cacaagaaat tgtgcctgat tttttaacag cggcaagatg ccgtaaatca gatgctacaa
     9481 aatgtaaagt tgtgtctttc tggtgactta cgcactatcc agacttgaaa atagtcgcgt
     9541 aacccatacg atgtgggtat cgcatattgc gttttgttaa actgaggtaa aaagaaaatt
     9601 atgatgcgaa tcgcgctctt cctgctaacg aacctggccg taatggtcgt tttcgggctg
     9661 gtactgagcc tgacagggat acagtcgagc agcgttcagg ggctgatgat catggccttg
     9721 ctgttcggtt ttggtggttc cttcgtttcg cttctgatgt ccaaatggat ggcattacga
     9781 tctgttggcg gggaagtgat cgagcaaccg cgtaacgaaa gggaacgttg gctggtcaat
     9841 actgtagcaa cccaggctcg tcaggcgggg atcgctatgc cgcaagtggc tatctaccat
     9901 gcgccggaca tcaacgcttt tgcaaccggt gcgcgccgtg atgcctctct ggttgctgtc
     9961 agcaccggtt tgctgcagaa catgagcccg gatgaagccg aggcggtaat tgctcacgaa
    10021 atcagccaca tcgccaatgg tgatatggtc accatgacgc tgattcaggg cgtggtgaac
    10081 accttcgtta tctttatttc ccgtattctg gcgcagcttg ccgcgggttt tatgggcgga
    10141 aatcgtgatg aaggtgaaga gagcaacggc aacccgctga tctactttgc ggttgcaacg
    10201 gttctggaac tggtgtttgg tattctggcg agcattatca ccatgtggtt ctcgcgtcat
    10261 cgtgaattcc atgctgatgc cggttcggca aaactggttg gtcgcgagaa aatgattgcc
    10321 gcgctgcagc gcctgaaaac cagctatgaa ccgcaagaag caaccagcat gatggctttc
    10381 tgcattaacg gtaagtcgaa atcgctcagt gagttgttca tgactcaccc gccgctggat
    10441 aaacgtattg aagctctgcg tacgggtgaa tacctgaagt aatatgaagt aaatactaaa
    10501 aagcgcgtcc tgatcgacgc gcttttttta tgccctggct cgtggctgag tgatacgtaa
    10561 accactgaca caggcagcaa tcactgccag aatagccgca gccatcagcg agacgtgcgt
    10621 accattatca ccaaactgat ttagcatcag cgccaccagc gccgcgccac tactctgacc
    10681 cagtagacga gccgttccta acatgccact ggccccaccg ctacgttcgc gcggagcgga
    10741 ggtaataatg gtgtggttat tgggtgactg gaataagcca aatccagcgc cacataagat
    10801 catcggccag ataatattga tatccgcagg tgacgcgggc agcagaacca gggaaaaaag
    10861 ccccgtagcc atgatgaaca accctaaagc acccagcaat cctgcatgta cgcgttcaat
    10921 caaatagcct gccagcggag ccatcaccat cgttgctaac ggccacggtg tcagaagtaa
    10981 acctgtttcg acttcactac gcccgagcac ggtttgcagg taaaagggca gggaaaccat
    11041 tgccagcatt tgtgcgcaga aagagcaaac agatgtgcaa atagaaagtg aaaacagcgg
    11101 gatacgcagt aaatccaccg gtagcagcgg tacgggaaga gaaagctggc ggcgaataaa
    11161 gaaaatacca acaacaacca ttaccaccag ttccgcagca attaacgtca gcgattgccc
    11221 ctgagcgaaa ccactcaacg cagtgataag caggccgaag gttaacgcgt tcatcacggc
    11281 gctgggcagg tcgaaacggg gtttactggc gcgagaacca ttgggtggca gaaaacgcat
    11341 cgccagaagc agggcgataa tacctaacgg tacgttgatt aaaaataacc atttccagga
    11401 tgcgatggag aggattgctg cagcaattgt cggcccggca gcagaagaga cggcaacaat
    11461 aaacgagttt atgcccatcc ctctccccag aaaacgttgt ggatagatca ggcggataag
    11521 tgcggtatta acgctcatca acgctgcacc gccgaaacct tgtatgacac gcgcaagggt
    11581 gagcatttgc agcgaatcag aaagggcgca gaacagtgaa gacaacagaa aaacgaccag
    11641 accgcattta taaatacgtc gatagccaaa catatcgccc agaaacgaaa acgagagcag
    11701 ggagatgaca atggcgattt gataggcgtt cactacccag atggaactgg ctggtgtggc
    11761 atgaaggtcc gtggcaattg ttggcagggc gacgttggcg attgcgccgt caaggacagc
    11821 catcgaaata ccaatcacaa tggttaatat cgcaccgtat cgctggggca atggcaggcc
    11881 gtcggcctga acttttggca taggaatttt atatctttgg tgaataatca acaggggtat
    11941 gattctagca ctgatattca cggtggatgt cgcagaattg taactaaccg gcaaaagatg
    12001 gattgcgacc accctggtgc gaaattataa taaaaaacag ttctgatttt tataaaacac
    12061 tcgcaatgag gtgataaatg gctaacgcag atctggataa acagcctgat tctgtatctt
    12121 ccgtgctaaa agtttttggc attttgcagg cgctgggtga agagcgcgaa atagggataa
    12181 ccgagctgtc gcagcgcgtc atgatgtcaa aaagcaccgt ttatcgcttt ttacagacca
    12241 tgaaaacctt aggttatgtg gcgcaggaag gggagtcgga gaaatattcc ctgaccctga
    12301 aattgtttga actgggcgct cgcgcgttac aaaacgtcga tttaattcgt agcgcagata
    12361 tccagatgcg tgagatctcc cgcctgacca aagaaactat ccacctcggc gcactggacg
    12421 aagacagtat tgtttacatt cacaaaattg actctatgta caatttgcgc atgtattcac
    12481 ggattgggcg tcgtaatccg ctgtacagca ccgcgattgg taaggtactg ctggcatggc
    12541 gcgatcgcga tgaagtgaag caaattcttg agggcgtgga gtataaacgc agtaccgagc
    12601 ggaccatcac cagtacagaa gcgttattac ccgttctgga ccaggtgcgc gagcaggggt
    12661 atggagaaga taacgaagag caggaagaag ggctgcaatg cattgcggta ccggtatttg
    12721 atcgctttgg cgtggtcatt gccggtttga gcatctcctt cccgacgttg cgtttctctg
    12781 aagagcgttt acaggaatat gtcgcaatgt tgcataccgc agcgcgcaaa atttctgccc
    12841 aaatgggtta tcacgactat ccgttctgat gagagtaaga acctgtcgga atatcaaaca
    12901 gacaggttct ttatttagca tgagaaaaat aaagttgaag gtgcgttata ttaaacgcgc
    12961 ttgctataag aatattttac tcatgagtga gaatctggtt atttattacc cttaaccatt
    13021 atcgaccacg atattgcttt tacgtaacag tgggcaatct gttatctcca agaaaccact
    13081 tttagtgtgc aagtattgta ccgtgctggt gcctctggca gtcagatagg tacattgcaa
    13141 acctaatcct gcggcattct ctttgcttcc aatcaaaacg ccatatccgc tgagtaataa
    13201 tcctatccat accagtgcta tcagcataac tgtgcgaatg atgaatcgca ttacaatctc
    13261 ttctcttttt atgttcgctt aatcgtagcg gcaatatgcg ctgaagcaag ctactcattc
    13321 cgaaaaagca cgaatatcga catagttagg cgctgtttaa ctaacgcatg ctagtttaat
    13381 gacataaggt aggtgaagcg gagattggag tgaaaaagtt tcgatgggtc gttctggttg
    13441 tcgtggtgtt ggcttgcttg ctgctttggg cgcaggtatt caacatgatg tgcgatcagg
    13501 atgtacaatt tttcagcgga atttgtgcca ttaaccagtt tatcccgtgg tgatatcatt
    13561 ttttcagcac tagattttct tcctctccag cagtggtaga atagcgcctc tactcagagg
    13621 tggtgaaatg aacgaagttg taaattcagg cgtgatgaac attgcgtctt tggttgtatc
    13681 ggtggtggtt cttcttatcg ggctcatctt gtggtttttt atcaatcgtg ccagttcacg
    13741 gactaacgaa cagattgaac tgcttgaggc gttgctggat cagcaaaaac gtcaaaatgc
    13801 actgttacgt cgtttgtgcg aagcaaacga accagagaaa gcagataaaa agaccattga
    13861 gagtcaaaaa tcggttgatg acgaagatat tattcgcctg gtcgccgaac gataatcctt
    13921 cctcaatctt gagtaacccc gtcgttgaag ggttactcaa ttcatcctga ttatacgcac
    13981 tgtcatggtg caactgcttc aaattaaaac tctggtctga cttcaaataa ttatcatatt
    14041 atttattagc gaaacgtttc tctcttgatt tttttgctca ctcatcaatt tttcttattt
    14101 taaatttaca atccctttgg ggattgactt ctctttaggg taattgatag ccgttaactg
    14161 actgttttat gagaaaaagt gatataactt tttattcatt gcatagcaga aaatgtgata
    14221 ttgcacgcac tatgtaataa cttctccctc tggcctggaa caactgaact tattgaacta
    14281 tgttagaaac tacgccagtt taagtatctg cctgaactgg caaggttaag cacaatgata
    14341 tatcggcgcg tattccgttg cataagtgtg caaaaaaagt ggaagacgta tcgagatttg
    14401 tgcgtctgat cgagacatgt ttaaaaatgg cttgccataa ttaacgttgt atgtgataaa
    14461 agatttcggg ttaaacgagg tacagttctg tttatgtgtg gcattttcag taaagaagtc
    14521 ctgagtaaac acgttgacgt tgaataccgc ttctctgccg agccttatat tggtgcctca
    14581 tgcagtaatg tgtcagtttt atctatgtta tgcctgcggg cgaagaaaac aatctaagga
    14641 atttttcaaa tggcaaagat taaaggtcag gttaagtggt tcaacgagtc taaaggtttt
    14701 ggcttcatta ctccggctga tggcagcaaa gatgtgttcg tacacttctc cgctatccag
    14761 ggtaatggct tcaaaactct ggctgaaggt cagaacgttg agttcgaaat tcaggacggc
    14821 cagaaaggtc cggcagctgt taacgtaaca gctatctgat cgaatccact gatctgaagt
    14881 gtgaatacgc ttcaatctcg ctataaagcc tcgtcgaatg cgaggctttt tactatgctt
    14941 tatcttcgct cctggcgttc ggatatttgc ccgccgcgtg attcgcgtta cacttgcggc
    15001 ctttagtatc ctgctggagt tgtcatgtcg ttttcctgtc cactttgcca tcagcctctt
    15061 tcgcgtgaaa aaaacagcta tatctgtccc caacgacatc agtttgatat ggcgaaagaa
    15121 gggtatgtca atctgctgcc cgttcagcat aaacggtctc gtgatccggg cgacagcgcg
    15181 gaaatgatgc aagcacgccg cgcattctta gatgccggac attatcagcc gctgcgtgat
    15241 gcaattgtcg cccaactgcg ggaacggctt gatgaaaagg ccacggcggt gctggatatt
    15301 ggctgtggtg aagggtatta cacacacgca tttgccgatg cgttgcccga aatcaccacg
    15361 tttggcctgg atgtctcgaa ggtagcgata aaagcggcgg cgaaacgcta tccgcaggtc
    15421 actctttgtg tcgcttccag ccaccgtttg ccgttttccg ataccagtat ggacgccata
    15481 atacgtattt acgcgccgtg taaagcagaa gaattagcac gagtagtgaa gcccggcggc
    15541 tgggtcatta ctgccacgcc gggaccgcga catttgatgg agctgaaggg gctgatttac
    15601 aatgaagtac atcttcatgc acctcatgca gaacaactgg aaggttttac attacagcag
    15661 agtgcggagt tgtgttatcc gatgcgtctt cgcggtgatg aagccgtcgc attattgcag
    15721 atgacgccgt ttgcctggcg tgcgaagcca gaagtctggc aaacactggc agcaaaagaa
    15781 gtgttcgact gccagacgga ctttaatatt cacctctggc agcgttctta ttaaccgtgg
    15841 aagtgcgtcc agaggatctg gacgccgatg ccgatcagca ccagcccgcc gagaatttcc
    15901 gcttttttcc caataattga gccgataaag cgaccaacca tcatccctaa tgtcgacata
    15961 atcaaggttg cacaaccaat ggccaatgcg gtcgtgataa tgttgacctg aaggaaagca
    16021 agaccaacac ccacagccat ggcatccagg ctggtggcaa tcgcggtggt taccagtagc
    16081 cagaaaccgt gccgacggcg cggctcttca tcttcatcat ctgcgccacg aaaaccctca
    16141 ataatcattc gcccgccgag gaatatcagc agcacaaacg caatccagtg attccattca
    16201 aggacaaacc ggctggctaa catgcccatt c
//
LOCUS       DAJTUN010000093        15801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTUN010000093 DAJTUN010000000
VERSION     DAJTUN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.093"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1674)
                     /gene="speA"
                     /locus_tag="OO470_002812"
     CDS             complement(<1..1674)
                     /gene="speA"
                     /locus_tag="OO470_002812"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6252007.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLERLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPP"
     gene            complement(1683..1814)
                     /gene="yqgB"
                     /locus_tag="OO470_002813"
     CDS             complement(1683..1814)
                     /gene="yqgB"
                     /locus_tag="OO470_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6252008.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            1950..2165
                     /locus_tag="OO470_002814"
     CDS             1950..2165
                     /locus_tag="OO470_002814"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252009.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2469..3623
                     /gene="metK"
                     /locus_tag="OO470_002815"
     CDS             2469..3623
                     /gene="metK"
                     /locus_tag="OO470_002815"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6252010.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4060..5454
                     /gene="galP"
                     /locus_tag="OO470_002816"
     CDS             4060..5454
                     /gene="galP"
                     /locus_tag="OO470_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6252011.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5531..6028
                     /locus_tag="OO470_002817"
     CDS             5531..6028
                     /locus_tag="OO470_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6252012.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6123..6830
                     /gene="endA"
                     /locus_tag="OO470_002818"
     CDS             6123..6830
                     /gene="endA"
                     /locus_tag="OO470_002818"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6252013.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            6910..7641
                     /gene="rsmE"
                     /locus_tag="OO470_002819"
     CDS             6910..7641
                     /gene="rsmE"
                     /locus_tag="OO470_002819"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6252014.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7654..8604
                     /gene="gshB"
                     /locus_tag="OO470_002820"
     CDS             7654..8604
                     /gene="gshB"
                     /locus_tag="OO470_002820"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6252015.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8713..9276
                     /locus_tag="OO470_002821"
     CDS             8713..9276
                     /locus_tag="OO470_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6252016.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9276..9692
                     /gene="ruvX"
                     /locus_tag="OO470_002822"
     CDS             9276..9692
                     /gene="ruvX"
                     /locus_tag="OO470_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6252017.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(9868..10848)
                     /locus_tag="OO470_002823"
     CDS             complement(9868..10848)
                     /locus_tag="OO470_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6252018.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            10866..11570
                     /gene="yggS"
                     /locus_tag="OO470_002824"
     CDS             10866..11570
                     /gene="yggS"
                     /locus_tag="OO470_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6252019.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11588..12154
                     /gene="yggT"
                     /locus_tag="OO470_002825"
     CDS             11588..12154
                     /gene="yggT"
                     /locus_tag="OO470_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6252020.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12151..12441
                     /locus_tag="OO470_002826"
     CDS             12151..12441
                     /locus_tag="OO470_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6252021.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12449..13042
                     /locus_tag="OO470_002827"
     CDS             12449..13042
                     /locus_tag="OO470_002827"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6252022.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13035..14171
                     /gene="hemW"
                     /locus_tag="OO470_002828"
     CDS             13035..14171
                     /gene="hemW"
                     /locus_tag="OO470_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6252023.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14485..15448
                     /locus_tag="OO470_002829"
                     /pseudo
     CDS             14485..15448
                     /locus_tag="OO470_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15493..>15801
                     /locus_tag="OO470_002830"
     CDS             15493..>15801
                     /locus_tag="OO470_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6252024.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     S"
BASE COUNT         3883 a         4090 c         4004 g         3824 t
ORIGIN      
        1 cggcggattc tctggatcgt actccggcat tggcattgtc gtggcaatac catcaccatc
       61 aatatagtgg tcgatagcac cgtcagagtc acaggtaata tccagcagca cagcgcgacg
      121 ttccggcact tgatccagcc tttccagcgg tagaaccggg aacaactggt cgatccccca
      181 tgcgtccggc attgactgga acagcgagaa attgacgtac attttgtccg ccatacgttc
      241 ctgcagttcg tcgattatcg gacgatgcgc acggttttgc ggatccagtt gcttctgcac
      301 ttcatggcac atgctcagat agagttgttc tgcccatgca cgttcttgca ggctaaaggt
      361 gccggaagag tagccgatat gaatgtcgtg cagatccatc tggctgtcgt gtaaccattc
      421 acgcaaagaa cggcgagttc ccggttcgtg catttcctgc caggtttccc acatgctttg
      481 cagcgcacgc ggcgcatctt ctacgggcgc ggtcggcacc gtatattcgt tacgttccac
      541 gccgatgata ttcgacacca gcacggtatg gtgcgcagtc accgcacggc ccgattcggt
      601 gattaccgtc ggatgcggca gaccattttc ttcacacgca tcgccaatcg cccagatgat
      661 gttgttggcg tattcattga ggccatagtt tacggagcag tcagactgcg aacgggtgcc
      721 ttcataatcc acgcccagac cgccgccgac gtcgaagcac tgaatattga cgcccagctt
      781 gtgcagttcc acatagaaac gcgcggattc acgaacgcct gtcgcgatat cgcgaatatt
      841 cgccatctgc gaaccgaggt ggaagtgcag tagttgcagg ctgtcgagac gcccggcttc
      901 acgcagggtt tcaaccagtt gcagtacctg agtcgcagcc aggccgaact tcgatttttc
      961 cccgccggag gactgccatt tacccgagcc ctgcgaagcc agacgtgcac gcacgcccag
     1021 acgaggaacg acattcagac gttctgcttc atccagcaca atggcgattt ctgacatctt
     1081 ctcaatgacc agatagacct tgtgccccat cttctcgcca attaatgcca agcggatata
     1141 ttcgcggtct ttataaccgt tgcagacgat gacgctacga gtcatgccag catgtgccag
     1201 cactgccatc aactcggctt tggaaccggc ttccagaccc agcggttcac ccgaatgaat
     1261 cagggactcg atcacgcggc ggtgctggtt aactttgatc ggataaacaa ggaagtaatc
     1321 gccgttatag ccgtaggatt ccctcgcacg tttgaacgcg gcgttaatgg aacgcaaacg
     1381 gtgctgcagg atctgtggga aacagaacag tgcaggcaga cgctgaccct gtgcttcacg
     1441 agttttcact aactgcgcga gatcgacgcg agcttccggg acgtccgggt ccgggcacac
     1501 gctgatgtgg cccagctcgt taacgtcata gtagttattg ccccaccagg caatattgta
     1561 agtacgcagc atcttgctgg cttcctggga gctcattgca acctcctgca tggagcgtag
     1621 tacaccgtgt tcgcccgctg acgaaggcaa acccatagac atgtcgtcag acatagcgaa
     1681 cctcaaatta ttttattaag tgtaaaacag ttaacgactg tcgcagcctg gaactgcgat
     1741 aacaacccat aagcacatgg attttccagc agtgtatgct gacgctccga ctgcgcaacc
     1801 ggtttctttt tcataacatt attaagcaca taaccgaacg taagtgtgac agttcggcga
     1861 aaccacgaga aaactcttgt ttttacaaga gcgcccttgt tcagtcctca gtaactgtaa
     1921 ccagctcttg aatcctgaga agcgccgaga tgggtataac atcggcaggt atgcaaagca
     1981 gagatgcaga gtgcggggaa cgagtcttca ccagaacggt gagacaggtt aagcagcaga
     2041 caacggttca ttatttcgta tcacctccac ggccgcctgt taagacgaac ccacaagcca
     2101 aaactctgat ttcaacccgg ctggaagtgg caacacgaaa gaaacgtcgt gtgcttttta
     2161 tttaagccgc gcgccgcgtt ttatacccca caatggcaga aaattgcaaa agataaatac
     2221 gcagaatgcc ggcattgtca ggaaaaattt ccagccacgt ttttaacaga atgagacacg
     2281 attcaaaaaa aagtggaaat cgggtgaaga attgacctaa aatagccatc cagatgttaa
     2341 tccatccata ccgattaaca ctcagactgc cagtgttttt aacctgcaga gtcgtggtag
     2401 gatccgctac cacagaaaat ccacacaaca gtttgagcta accaaattct ctttaggtga
     2461 tattaaatat ggcaaaacac ctttttacgt ccgagtccgt ctctgaaggg catcctgaca
     2521 aaattgctga ccaaatctct gatgccgttt tagacgcgat cctcgaacag gatccgaaag
     2581 cacgcgttgc ttgcgaaacc tacgtaaaaa ccggcatggt tttagttggc ggcgaaatca
     2641 ccaccagcgc ctgggtagac atcgaagaga tcacccgtaa caccgttcgc gaaattggct
     2701 atgtgcattc cgacatgggc tttgacgcta actcctgtgc ggttctgagc gctatcggca
     2761 aacagtctcc tgacatcaac cagggcgttg accgtgccga tccgctggaa cagggcgcgg
     2821 gtgaccaggg tctgatgttt ggctacgcaa ctaatgaaac cgacgtgctg atgccagcac
     2881 ctatcaccta tgcacaccgt ctggtacagc gtcaggctga agtgcgtaaa aacggcactc
     2941 tgccgtggct gcgcccggac gcgaaaagcc aggtgacttt ccagtatgac gacggcaaaa
     3001 tcgttggtat tgatgctgtc gtgctttcca ctcagcactc tgaagagatc gaccagaaat
     3061 cgctgcaaga agcggtaatg gaagagatca tcaagccaat tctgcccgct gaatggctga
     3121 cttctgccac caaattcttc atcaacccga ccggtcgttt cgttatcggt ggcccaatgg
     3181 gtgactgcgg tctgactggc cgtaaaatta tcgttgatac ctacggcggc atggcgcgtc
     3241 acggtggcgg tgcattctct ggtaaagatc catcaaaagt ggaccgttcc gcagcctacg
     3301 cagcacgtta tgtcgcgaaa aacatcgttg ctgctggcct ggccgatcgt tgtgaaattc
     3361 aggtttccta cgcaatcggc gtggctgaac cgacctccat catggtagaa actttcggta
     3421 ctgagaaagt gccttctgaa caactgactc tgctggtacg tgagttcttc gacctgcgcc
     3481 catacggact gattcagatg ctggatctgc tgcacccgat ctacaaagaa accgcagcat
     3541 acggtcactt tggtcgtgaa catttcccgt gggaaaaaac cgacaaagcg cagctgctgc
     3601 gcgatgctgc cggtctgaag taatctttct tcacctgcgt tcaaaggcca gcctcgcgct
     3661 ggcctttttc ttttacctca ccgcggtgga taggcgttca cgccgcatcc ggcaaaaaaa
     3721 ctgcccgcac aataacatca ttcttcctga tcacgtttca cggcaaatta ccatcacaac
     3781 tgaaaccgat tacaccaacc acaccagcaa aatatttgta atattttcat attattattc
     3841 agctttcaca gttgttacat ttcttttcag taaagtctta attgcagatg acagcgttta
     3901 atctatgatg atataactca attattttca tgcccttaaa tcataactaa gataaatgtt
     3961 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
     4021 cttaattcac aataaaaaat aactatattg gagggcatca tgcctgacgc taaaaaacag
     4081 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
     4141 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
     4201 cagataactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
     4261 ggtgcggtgg gcatcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
     4321 ggcgcaatcc tgtttgttgc cggttcactg ttctctgcgg ctgcgccaaa cgttgaagta
     4381 ctgattcttt cgcgcgttct gctggggctg gcggtgggtg tggcctctta taccgcacca
     4441 ctgtacctgt cagaaattgc gccggaaaaa attcgcggca gtatgatctc gatgtatcag
     4501 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
     4561 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
     4621 gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc
     4681 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
     4741 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaatagcaac
     4801 ttccgccgcg cggtgttcct tggcgtactg ttacaggtaa tgcagcaatt caccgggatg
     4861 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccacc
     4921 gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca
     4981 atcggccttg ttgaccgctg gggacgtaaa ccaacgctaa cgctgggctt cctggtgatg
     5041 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
     5101 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
     5161 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
     5221 acctgctcca ccgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
     5281 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
     5341 tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
     5401 gaacgtaatc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
     5461 caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc
     5521 ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgccg
     5581 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
     5641 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
     5701 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
     5761 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
     5821 agagtggaag tggatgatgg aaagcgtgct gggcgttccc gcccgtcgta cgcatcagtt
     5881 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgtaagtgcc aggaacatca
     5941 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccatctatc gctgtgttca
     6001 ctgcggtgaa cagctggttg cgaaataacc acctgaacta tcaggaactt tcctgatctg
     6061 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
     6121 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccga
     6181 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
     6241 ctgatgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
     6301 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccgc gccagccgcg
     6361 tagagtggga acacgtcgtt cccgcctggc aattcggtca ccagcgccag tgctggcagg
     6421 acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgc
     6481 ataacctgca gccgtcagtc ggcgaggtga atggcgatcg cggcaatttt atgtacagcc
     6541 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
     6601 aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
     6661 gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca
     6721 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcagg
     6781 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
     6841 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
     6901 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
     6961 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg
     7021 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
     7081 agcaaaaaaa gcgtggaagt gaaggtgctg gaaggccaga tcgacgatcg cgaatctccg
     7141 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tactatccag
     7201 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
     7261 aaactggata gtgaacgtct gaacaagaag cttcagcagt ggcagaagat tgcaattgct
     7321 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
     7381 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
     7441 agcatcaata cgttgccttt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc
     7501 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
     7561 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
     7621 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
     7681 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
     7741 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
     7801 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc
     7861 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
     7921 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
     7981 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
     8041 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
     8101 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
     8161 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
     8221 catggcactt gctactgcat ggcgcaaaat tacctgccag ccattaaaga tggcgacaaa
     8281 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
     8341 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
     8401 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
     8461 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
     8521 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
     8581 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
     8641 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
     8701 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
     8761 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
     8821 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
     8881 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggc
     8941 ccactggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
     9001 attcgcattt cagacaacac ggtgatgacc acttcccgcg atgtgctgga aacgctcggc
     9061 accgataaac aaccgtctga cgtattggtg gctctgggct atgcctcctg ggaaaaaggt
     9121 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
     9181 ctgttcaaaa cgccgattgc cggccgctgg cgcgaggcgg caaaactgat tggtgtggat
     9241 attctcacca tgcctggtgt ggcaggacac gcctgatgag tggaacctta ctcgccttcg
     9301 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc accgctcgcc
     9361 ctttgcctgc aattaaagcg caggacggta cgccggactg gaacctcatc gaacgtttac
     9421 tgaaagagtg gcagcctgac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
     9481 aacagccgtt aaccgccaga gcgcgcaaat ttgccaaccg tattcatggt cgtttcggtg
     9541 ttgaagtaaa gctccatgac gagcgtctta gcactgtgga agcccgttcc ggtctttttg
     9601 aacagggcgg ctatcgggcg ctcaacaaag gcaaagttga ctctgcctct gcggttatta
     9661 ttctcgaaag ctatttcgag cagggatatt aaggcgatct aaaagcctgg cggatagtcg
     9721 ttaccgccat cttgccggat gcggcgtgaa cgccttatcc agcacacatc tggcagcggc
     9781 tgtaggtctg ataagacgcg ttagcgtcgc atcagacatt tattgccttt ataggcccta
     9841 taaacttgcg catcgggcat ggcaacgtca caaacgccct tcccccaccc gctgctgata
     9901 actctgctga aacgttaaca tccccacctg ctgcccggtt tgaataacat gcggtaactg
     9961 gtgggttttc ccttcgcgaa tcaaattccc caccgcggga gtgttaatca gtaattcaaa
    10021 cagcgccacg cgtccttcct gtttatccac ttccagcttt tgtgacagca ctgcccgtaa
    10081 actacccgca agttgattac gtacgggatc tttttcctgc gccggaaatg aatccaccag
    10141 tcgctcaact gcctgcgcgg caccacgtgt atgtaaggtt gccagcacca gatgcccggt
    10201 ttctgccgcc gtcagcgcca gacggattgt ctcactgtca cgtagctctc cgagcaaaat
    10261 cacatcagga tcttcccgca atgcagcccg caaccccgat gcgaacgtca tacagtgcaa
    10321 accaatttcc cgctgctgga tcaaacatcg ctggctggca tagagatatt ccacaggatc
    10381 ttccagcgtc agaatatgcg catcggcatg ttgattaaga tagccaacca tcgccgccag
    10441 cgtggtagat ttgccactcc ccgtagcccc cgtcaccaga atcaggccat tctcgctctt
    10501 gagtaattcc ggcaataccg gtggtgcacc aagctgttcg agctgtggac agtgcgaagg
    10561 taacaaccgt aatgccagag aaataccttg ccgttgcgcg aacgcactgc cacgcaaccg
    10621 ctggttttcc gccagcgaca cggcaaaatc cagctggcca ttttccagca atattgcccg
    10681 ctgatcgtca tccagccact cccgcagtag ctcttcgacg tccggcgcat caaacggcgc
    10741 agcttccatt ctgccacgaa tgcgccatcg tgcgggccag gcgctgcaca ggtgtagatc
    10801 cgagacgtta tgctttacac taagggccac aatttcttcc atattcatac taagatcctc
    10861 ggaaaatgaa cgatattgcg cataacctgg cacaggtccg ggacaaaatc tcagcggctg
    10921 caacgcgttg cggccgttct ccagaagaaa ttacgctgct tgcagtcagt aaaacaaaac
    10981 ctgcgagcgc catcgcagaa gccattgatg ccgggcagcg tcaatttggt gaaaactacg
    11041 ttcaggaagg ggtagataaa attcgccact ttcaggaact gggcgtaaca ggattagaat
    11101 ggcattttat tggcccgttg cagtctaata aaagccgcct agtggcagag catttcgact
    11161 ggtgtcatac catcgaccgt ttgcgcattg ctacccgcct caacgagcag cgcccggcag
    11221 aacttccccc tcttaacgta ctgattcaaa ttaatattag tgatgaaaac agtaagtccg
    11281 ggattcaact ggcagaactg gacgagctgg cagcggcggt cgctgaacta ccgcgtttac
    11341 gtctgcgcgg gctgatggca atccctgcgc ctgagtcaga atatgtaagg cagtttgaag
    11401 ttgcacgcca aatggctgta gcatttgccg gactgaaaac gcgctacccg catatcgaca
    11461 cgctctctct gggaatgtcg gacgatatgg aagccgccat tgcggcaggt agcacgatgg
    11521 ttcgtatcgg cactgcaatt tttggtgcgc gtgattactc taaaaaataa ggaattaaag
    11581 gaacgccatg aatacgttga ctttcctgct ttcaacggtc attgagctgt atacaatggt
    11641 gctgttatta cgcatctgga tgcagtgggc tcattgtgat ttttacaacc ccttctcaca
    11701 gtttgtagtg aaggtagcgc agccaattat cgggccactg cgccgcgtta ttccggcaat
    11761 gggtcccatt gacagcgcct cgctgctggt tgcctatatt ctcagtttta tcaaagccat
    11821 tgtgctgttt gaagtggtga ccttcctgcc aatcatctgg attgccggtt tactgattct
    11881 gctgaaaacc atcggcttgc tgattttctg ggtcctgctg gtgatggcga ttatgagctg
    11941 ggtaagccag ggtcgtagcc cgattgaata cgtgctgatt cagctggccg atccgctgct
    12001 gcgcccgatt cgccgcttgc taccggcaat gggtgggatt gatttctcgc cgatgatcct
    12061 cgttctgctg ctgtatgcca tcaatatggg tgtcgcagaa gtattacagg caaccggaaa
    12121 catgctgctg ccggggctgt ggatggcgtt atgagtgccg taacagttaa tgatgacggt
    12181 ctggttttac agctctatat tcagccgaaa gccagccgtg attctattgt cggtttacat
    12241 ggcgacgaag ttaaagtcgc cattaccgcg ccgccggttg acggccaggc caacagtcat
    12301 ctggtgaagt ttctcggtaa gcaattccgg gttgccaaaa gccaggtggt gattgaaaaa
    12361 ggcgaacttg gccgccacaa acaaattaaa atcattaatc cgcaacaaat cccgccagaa
    12421 atcgcggcgt taattaatta ggtatcctat gcaaaaagtt gtcctcgcaa ccggcaatgc
    12481 cggtaaagtg cgtgagctgg cgtcgctgct tagcgacttt ggtcttgata tcgtggccca
    12541 aacagacctc ggcgttgatt ccgctgaaga aaccggccta acctttatcg aaaacgcgat
    12601 tctgaaagcg cgccatgcgg caaaagtgac cggtttaccg gcaattgccg acgactctgg
    12661 tctggcggta gatgcgctgg gtggcgcgcc gggtatttac tccgcgcgtt attccggtga
    12721 agacgcgacc gatcaaaaga atctgcaaaa actgttggaa acactgaaag acgtaccgga
    12781 cgaccaacgt caggcgcgtt tccactgcgt gctggtatat ctgcgtcacg cggaagatcc
    12841 gactccgctg gtatgccacg gcagctggcc gggcgtgatt actcgcgaac cagcgggcac
    12901 tggtggcttt ggttatgatc caatcttctt cgtaccttcc gaagggaaaa ccgccgccga
    12961 actgacccgc gaagaaaaga gcgccatttc ccaccgtggt caggcgttga aactgctgct
    13021 ggacgcttta cgtaatggtt aaattaccgc cgctgagtct ctacattcac atcccgtggt
    13081 gcgtgcagaa atgcccgtac tgcgatttca actctcacgc gttgaaagga gaagtgccgc
    13141 acgacgatta tgttcagcat ctgctttgcg atctggacaa tgatgtggct tacgctcagg
    13201 gccgtgaagt aaagacaatc tttattggcg gtggtacgcc gagcctgctt tccggcccgg
    13261 cgatgcaaac gctgctagac ggcgtgcgtg cgcgtttgcc gctggcagcg gatgcagaaa
    13321 ttactatgga agcgaaccca ggcacggtag aagcggatcg ctttgtcgat tatcagcgtg
    13381 ctggtgtaaa ccgcatctct attggtgtgc agagttttag cgaagaaaag ctgaaacgac
    13441 ttgggcgtat tcatggcccg caagaagcga aacgagcggc aaagctggcg agcggtttag
    13501 ggttacgtag ctttaacctt gatttgatgc atggactacc ggatcaatca ctggaagagg
    13561 cgcttggcga tctacgccag gccattgaac tgaatccgcc gcatctttcc tggtatcaac
    13621 tgactatcga acctaatacg ctgtttggct cgcgaccgcc ggtgctgccg gacgatgacg
    13681 cgctgtggga tattttcgaa caggggcatc agttattaac cgcagcgggt tatcagcaat
    13741 atgaaacttc cgcttacgcc aaacccggtt atcagtgcca gcacaatctc aactactggc
    13801 gctttggtga ctacatcggt attggctgcg gcgcgcacgg caaagtgacc ttcccggatg
    13861 ggcgcattct gcgtaccact aaaacgcgtc atcctcgtgg ttttatgcaa ggaaggtatc
    13921 tggaaagcca gcgtgatgtc gaagccgcag ataagccgtt tgagttcttt atgaatcgct
    13981 tccgtctgct ggaggccgcg ccgcgcgtgg agtttattgc gtataccggg ctttgcgaag
    14041 atgtgattcg cccacagtta gacgaggcga ttgcccaggg ttatctcacc gaatgtgcgg
    14101 attactggca gataacggaa catggtaaat tattcttaaa ctcacttctt gaattattcc
    14161 tcgctgagta atcattaatg cgcctgcggg cgcattaatc acttctttta tccccccgtt
    14221 aattaacctc ggcagaaata tatttcttac tgaataaata tcgacgacat ttattatata
    14281 tatttagtga atatttacta atcattcatt gattataaat atgtacaacc tggcgcacaa
    14341 atttaatatt taatttctga ctttatttca agttgtgata aacatagaca agttgtcaga
    14401 caggttaacc aatttgtcgc acaaatcact gataagcaca attagatatc aaccaatttc
    14461 catccttcaa cagggagttt ttttatgaaa atgaggtcat ttacccgctc attagtctgc
    14521 gcatccttgc tggctctggt tagcactggc gtaaacgccg cagaaaaagt ggcgttaaag
    14581 ttagcacaca acctggaaag aagtcatgtt gtacatcaga gttttgaaga gctggcgaaa
    14641 gaggtaaaac agctgtcaaa aggtaatatg gtgatccgta tttatcccag cagccagatg
    14701 ggtaatgcgc gagaaactat ggaattgttg caaaatggcg cactggatat gaccaaaggt
    14761 tccgccagtg atctggagtc ttttgataat atttatgcta tctataattt gccgttctta
    14821 ttaagttgtt tttggtgaag ttggtaaaga aattatggac tcaacaaaag acaaaggctt
    14881 ttttgcttta tcagcttatg tagcgggtac tcgcagtttc tatgcgaaaa aaccgattac
    14941 aaagccagaa gatcttaaag gactgaaaat tcgtgttcag ccaagtccga ccaccatcaa
    15001 aatgattgaa ctgatgggtg gttcacccac tccaatttca tttggcgaag tttatactgc
    15061 gatgcagcaa ggtgtagtag atggcgcaga aaataacgtg ccttcctgga tgcagactcg
    15121 acatattgaa attgccaaag ttttttctga agatgaacac gcctcaattc ctgacttttt
    15181 ggttatctcc accaaaacat ggaataaatt aaccccagaa cagcaacaaa tcctcgaaac
    15241 agcggcgaag aaatccgaag cttatcaaca aaaattgtgg gaaaaaattg acgctgacac
    15301 acgtgctcag gcgaaagcta tggggggtga aattgtaaaa gtggataaag cgccattccg
    15361 taaagccgtg caacctctgt ttgacgactt caaaaaagat ccaaaacaag ctgctttgtt
    15421 agagaaattc gacaacgctg ctcaataata ttccgggcca gcctctggcc ctgattttcc
    15481 aggatgctta caatgatatt taaccgcctg aaacttgcgg tagaccgcgt cattgccgcg
    15541 ttttctgtcg ccgtaatgct tgctctggtt gtatgcgtag tctggcaagt atttagccgc
    15601 tacgtactta accagccaag tacattaacc gatgagctgg cacgcttttt gatgatctgg
    15661 gtaggattat taggtgctgc atataccgtc ggcgcgcagc gacatctctc tattgattta
    15721 tttgctctcg ccctgaataa gcgcaaacaa ttacttctta gtatagtcat taacgttctc
    15781 attttaggtt ttgccggttc g
//
LOCUS       DAJTUN010000094        15483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTUN010000094 DAJTUN010000000
VERSION     DAJTUN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.094"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            62..1063
                     /locus_tag="OO470_002831"
     CDS             62..1063
                     /locus_tag="OO470_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6252025.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
     gene            complement(1354..1788)
                     /locus_tag="OO470_002832"
     CDS             complement(1354..1788)
                     /locus_tag="OO470_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6252026.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1760..2551)
                     /locus_tag="OO470_002833"
     CDS             complement(1760..2551)
                     /locus_tag="OO470_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6252027.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(2551..3231)
                     /locus_tag="OO470_002834"
     CDS             complement(2551..3231)
                     /locus_tag="OO470_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6252028.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(3228..4427)
                     /locus_tag="OO470_002835"
     CDS             complement(3228..4427)
                     /locus_tag="OO470_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6252029.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(4428..4781)
                     /locus_tag="OO470_002836"
     CDS             complement(4428..4781)
                     /locus_tag="OO470_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252030.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(4781..5521)
                     /locus_tag="OO470_002837"
     CDS             complement(4781..5521)
                     /locus_tag="OO470_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6252031.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            5744..6034
                     /locus_tag="OO470_002838"
     CDS             5744..6034
                     /locus_tag="OO470_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCR6252032.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(6058..6402)
                     /locus_tag="OO470_002839"
     CDS             complement(6058..6402)
                     /locus_tag="OO470_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252033.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(6415..7479)
                     /locus_tag="OO470_002840"
     CDS             complement(6415..7479)
                     /locus_tag="OO470_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252034.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(7482..7787)
                     /locus_tag="OO470_002841"
     CDS             complement(7482..7787)
                     /locus_tag="OO470_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252035.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(7789..8391)
                     /locus_tag="OO470_002842"
     CDS             complement(7789..8391)
                     /locus_tag="OO470_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252036.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(8391..10406)
                     /locus_tag="OO470_002843"
     CDS             complement(8391..10406)
                     /locus_tag="OO470_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCR6252037.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(10584..11009)
                     /locus_tag="OO470_002844"
     CDS             complement(10584..11009)
                     /locus_tag="OO470_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252038.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(11013..11453)
                     /locus_tag="OO470_002845"
     CDS             complement(11013..11453)
                     /locus_tag="OO470_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HCR6252039.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(11464..12609)
                     /locus_tag="OO470_002846"
     CDS             complement(11464..12609)
                     /locus_tag="OO470_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCR6252040.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(12613..13170)
                     /locus_tag="OO470_002847"
     CDS             complement(12613..13170)
                     /locus_tag="OO470_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252041.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(13145..13534)
                     /locus_tag="OO470_002848"
     CDS             complement(13145..13534)
                     /locus_tag="OO470_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HCR6252042.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(13521..14075)
                     /locus_tag="OO470_002849"
     CDS             complement(13521..14075)
                     /locus_tag="OO470_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252043.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(14072..14479)
                     /locus_tag="OO470_002850"
     CDS             complement(14072..14479)
                     /locus_tag="OO470_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCR6252044.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            14478..14712
                     /locus_tag="OO470_002851"
                     /pseudo
     CDS             14478..14712
                     /locus_tag="OO470_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14709..>15483)
                     /locus_tag="OO470_002852"
     CDS             complement(14709..>15483)
                     /locus_tag="OO470_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6252045.1"
                     /translation="NTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWG
                     MELGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGV
                     PVNNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSS
                     AGNQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLV
                     PLQSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
BASE COUNT         3625 a         4155 c         3788 g         3915 t
ORIGIN      
        1 aaatggacta tgacataaag atctgatatt ttcctcacaa aaacttaggg attttatttt
       61 aatgcataag agcaactgct ttgatacggc aaggcttgtg gcagctatga tggttctaat
      121 cagtcaccac tacgcgctct ctggacaact cgagccgtat atattcggct ttgaaagtgc
      181 tggcggcatt gctgttatca tattcttttc catatcagga tacctgattt caaaatcagc
      241 aatcagaagt gattccttta ttgatttcat ggctaaaaga gcaagaagaa tattccctgc
      301 tcttgtccct tgctctgtac tgacgtattt tttatttggg tggatgctta acaacttcag
      361 tacagaatat tttagtcatg acattatccg gaagaccata agctctattt taatgagtca
      421 ggctccagat gccgacatta catcacattt aattcatccc ggtattaacg gcagcttgtg
      481 gacactacca ctggagttta tgtgttacat aataactgga gtggctgttg ctctactaaa
      541 gagcggaaaa acgttcatag ttatactatt gatttttgtt tcactttcat tagttgaaag
      601 tgtatctgag aacaaggatg taatattctc cattccagta tggctgtatc ctttgcgggg
      661 gatggctttc ttttttggtg ccactatggc gatgtatgag aaatcttgga atgtaagtaa
      721 tgtgaaaatt tccgtgattt ctttgcttgc tatgtacgca tacgcgagct atggcaaagg
      781 cattgactat acaatggctt gctatattct ggtttctttc tcaacaatag ctatatgcac
      841 atcttttgga gatccactgg ttaagggtag gtttgattac tcttatggtg tatacattta
      901 tgcattcccc gttcagcagg ttattataaa tacattgcat attggctttt acccttcgat
      961 gttgttaagt gccattgtcg ttcttttctt ggctcatctt tcttggaatc taattgaaaa
     1021 aagattcctt acacgaagct cgccaaaatt atccctgaat tgaataataa aggggccatc
     1081 gagcccctga ataaatcaaa gccagatata acactgcctg gcagtggaca ccgttctgat
     1141 gcctcgtcct gcaaggacga gcgaaggtgg agttcttttc cacgcagcta tttagtacag
     1201 tactgagtca tattgtttat gtatgagtta acttccgtaa tatcaattaa ataaaataag
     1261 ttgaggccat tgtagaagtg agcattatat ccaggttatc tggacctgtt gaaaaatgcg
     1321 tattattcca acaattaccc tagattaaca agtctattta tttggatgca taggccactc
     1381 gggttttact acattaacac gcataagcag tacgcgatat tttttccatg ccagtaacag
     1441 tttcgtttca tcatctgttg ccatacctaa atcaaccgca tcctgaagcg gcgctattac
     1501 attcgttgcc tctgctatca gtgcagattt cttttcatca tttattaagg ctaattgttc
     1561 tggtgttgga gaaggacggt caacaatgac agggtgaccg ttctcaccac aggtaatcat
     1621 ttttgttaca ctctgtgagt taatcagtgt tttccactct gattccgaga tttccactgc
     1681 atcatcagga atattggtgc cgtgaaattc agtcgaataa aatccgtttg tcgatgcaga
     1741 ataaaaatat gacatttcgt taatatccca ttgcaatgta atacgcatca gcagatgaac
     1801 catatcctga accgggcgac actgtaatat caaatccagt gttatggaca tttgtcgccg
     1861 atacagtcgg ctgccctgac gcgccagtaa cgttcaatgt aactgtgaca gcaaggactc
     1921 ttgagaatgt catcgggaac ctcatcccgt atgttgaaga tacattcggg gagccattca
     1981 actgaccgca ctgtaaaatt aatccagacg gaaatttttg cgcagcagcc gttgcggtat
     2041 tacttgacgc gaacaggctc atatcaggta tctgattcgc ccctgtacct acattccttt
     2101 tagccgcttc tcccaaacca aggtatgtga gaatgtcagc aatagtattt ttcccaataa
     2161 tgtcacggcc aacagaagtt aaatcagtct gcgctgctgt atcatttcca gtgaaatatg
     2221 ggagtttatt tgcacctgtt gcgagcccag ctaatgccga cagcgtggca tcaagcgcct
     2281 ggaaatcttt cccgaaagcg gtccccattt tggatataaa cccgttcagg tctccatcat
     2341 caagcacatc cagcctgctt ttgttggcgg tgtactgcgc caacgctgcc gcgataaagc
     2401 tggcttgccg aatagctttg ttgacctgtg cgctggatgc tttacctgcc gtaaaccctg
     2461 aaagcagagc cggaagtgct tcccagtcag cttgtgaggt gacgttagcg ttcggatcaa
     2521 gcgcgaaagg tttaaagttg tttattgcca ttagagtatt gtcccccatg ctccaacatc
     2581 gaacccgccg atgtattcgt tatccatatc aaacccaaag aatttagagc cctcggatgg
     2641 tgcttctacc gaaggcgttt caacatcacc ggcccatacg ccagctgctt taacggtgag
     2701 atagccctgt ttgatagcgg caatcagttc aagagacaca tcagaaatat cagtctcggg
     2761 gaatacccaa accgatatcg tcatgtcctg gttgtcgaca atttgcatcc tcaggcctga
     2821 gccttcagtc gcagcgtcaa ggatgggagg cagagagtcg ttccgaccgt cccagttgtt
     2881 gatagcgatt ttcgctttca gaatgatgcg gtacgtctca tcgcttagcg tcgtatagcc
     2941 agaatcagga tcatatggcc ctttccagat gccctggtca tacccaagcc cgtcagtgtc
     3001 ccagctgaaa taaactccgc taattggctg gctgactatg cgactgcgtc cgatccacag
     3061 accgaggatg tcgagctgta caccgacagc agtatcaata tcgaaggctg ttataagccc
     3121 tgacatagtg ctggacacat caatcagcgg gcgggtgctc agatctatat ggtcaaaaaa
     3181 gagtggcttg gtagcgtggt agttagtgat cagttcggtg tatttgctca tgaggtcacc
     3241 gtgatactga tattcgcggt gctacaggac gcagaagcat cataggcaat atcaatgttt
     3301 gatgccgata cgctgccaga cgacttaccg atcagcaggt cggtaatatc gtaatagcgg
     3361 gcattcccgc cgctcacaac gccgaggttt gccggggaat aaatacggct cagcagaacg
     3421 tcgtcgccaa ttgttaggcc atttatataa tcggcaacag cctgtttaat ctgctcgccg
     3481 atttgagagg tatagccggt aaaaactttc agggtaatgg ctacgaaaat tggcacatcg
     3541 gtagagcgcg aaaaactgat gacgtgtgga ttaccgtaag tatccggcac cgtgacagaa
     3601 gttttaccgt aagttgcggt tccctgccct ttactccccc ggattgtttg ggctatctcg
     3661 gtaacatccc ctccatcgac gatggcggaa atagagtgtg gcggcagccc gttgctgtcg
     3721 gttgccccag tgtcgttctc atatagtttg tgacgtgtca cgccagcaac attagcgata
     3781 gcaccgtcga caccttcaaa cggtgtgatc gatggtagcg cgacgctttg cccctgccga
     3841 atgcgcagct ctgcgtcggt ttcggctggt gaaccgacag tagccgcaac tggattggtt
     3901 accgacaccc aacctcgggt cggggtgttg atagtggtaa tagtcccggc cagcgccgca
     3961 accgaaccgc tattcgcaca tgtggccgtc accagcacag taccatcaac gccgatcgct
     4021 acacttgcgg gaaaattcca gataatgccg tttttatccc gtgcggagcc attcgtgata
     4081 gtcgtgcctg ccgtaccggt taacagaagg tcagcagtag agtttgtcgc tacttttcgc
     4141 gtgatcccgt taattttcac attgctgcta agcgctgcgg cctgcgctgt cgtcggtgaa
     4201 aaagagttgt agatcccgat agcggtgttg ttagcgtcat gcacggcaag agccaccagc
     4261 gcgaccatct gcccgtcttt gctgtctggt tcgaggtagg catcactacc gtaaatctgc
     4321 ctgaaatagc tggtcagtgt atctaggatt gtctggtaat caggcgcact aatcccctgg
     4381 gcggttaccg ttgccgatag ccccagcgtg tcgaggttca aagccattta tgcctcgctt
     4441 gttacagtcg tctggccgta gattgtgtca atggaggaag tgaaggtgac gcgacggctg
     4501 gtgccgtcat aattggtatc gaaggaaaga atcgacagaa cgcccggtgt atcctgtatg
     4561 cgttcgcgta tagccaggat gtagacatct gatcgctgct tcccaagcac tgactgaaca
     4621 tacggcgtgc cttccgtcag atcgagaaac cactgaccgc gccacagctc gaaacgggtt
     4681 tttacggcct gggcgacaca ttccggactg tcgataagga aagtatcgtc accctggccg
     4741 aaagtgtaat cgccttcagt atcttcgcga cggtatcgca ttattgcggc cctccggtag
     4801 ttcccccgcc agtctgtact ccgccatgtt tatgcgtggc gacactgata ccagaagctg
     4861 ttacgtcatt cgttaccatc accggcccaa gcatcgtcgc agtaccgccg ctttcgccca
     4921 ttccctgaga caagttgcca ttaatcgtta cgttgccgtt cagcgtgata gtcggggatg
     4981 tgattgtcgt tccaccttca gccgtagctg taagctggcc cggtgtttta atggtgatgt
     5041 tatgtcctgc ggcgacttct acaaacgccg caccatcatc ggttcgcagc tgcgcggcgc
     5101 tggtactgat accgctgatt ttccgtgctt gcgactgcgg gccaacgatg gcgaacgcat
     5161 cagataagtc atgctggcgc gggtcgacgg tctcctgaac gccgccgctc tgccaccaaa
     5221 aatcgatgca acggtcagca aaaatcagga ggcactcgtc gccttctttt accggaaagg
     5281 tcagcgtgca accgccgccg cgcgggaaga tgaccggcac atccaccagc ggttttaatt
     5341 cggtggatcc atcgccaaca ataccgcgta gcgccacctc tactgtgcag gtaacagtct
     5401 caggatcgaa cgactgaatg atgccgggca tcgctacgcg catctgggta gacaccgaat
     5461 cggcaatggc ctgcgcggtc tgctgctcac cgccgatctg tgattgagtt ggaattggca
     5521 taaaaccccc ataaaaaaac cagccgaagc tggtttggta tcaattatct cttagtaact
     5581 aagctacatc agtgtgaagt aaggcttttc cgctgcaatg gcacacattc acaaggtgta
     5641 ttaggatatg tttgcaacaa gtaagcattg aaaatcaatt tcagccatga agccgccata
     5701 gagtggacct gcaccatcgc attattgact acaggagtaa caaatgggat ttagatttcg
     5761 taaaagaatc cggattgcgc ccggactcgc gatcaacatt agtaaaagcg gagtaagcac
     5821 ttcaattggt ggtaaaggtt ccaccattaa catcgggaaa aaaggcgtaa agatgacaaa
     5881 tggccttccc ggcacggggc tgtcgcatac cactaacctt tactctcccg ggaaatcgac
     5941 agaaaaaaaa caacttactc ataagcaaaa aataattaga aacatcctgt tcgtaattat
     6001 tgtgtttata ataattaaag ctcaatattt ttgacgcatg cccgcctatc tggcgggcta
     6061 ttttactttt cggcagtcgt atgttgcata ctgacgcggc gaatccatgc tggcttgcag
     6121 ccactgtgcg ttgagaatgg ctttgccgtt tcgattgatg tactcaagac caacccatct
     6181 tccaggctga tcggtagcca tacgccaatc catcttgata ttgttataat cgcctttgtt
     6241 tttcaggaag gtgatttttt gcatttctgg ctttgcgcca ttgattctgg ctaagccatc
     6301 atctgcccaa tggattttaa aatcaccaca ttgctgatcc gcaaaagcgg gtgctgatat
     6361 aagcaccgcg aacacggtaa cagtgcaaag taaatgtctc acggccccac ctcactaatt
     6421 cgtcgtccct tgcattgctt tcgggctata aagatcacga gcgccacgcg caaaacacat
     6481 caaatccatg taccacgcct gacctctggt gtcgccagta tagtcgatag ctttgacgat
     6541 ataaacgcca tccgtcgcaa tgctggcagc ctgtgacgtc gtgccggtca gcacacggtt
     6601 gccgttctct tctgtttcgg tgatacgccc gggcgactgt gctatttcgc tattgccgag
     6661 cgtggcgcgg tacaccgaag cctgatcgag ctggataaga ccattaatac ggatgtttgg
     6721 gtttatcagg catcgcacgt ttactccgcc acccatcgtt tgttgcggca taccgatcag
     6781 gccagtattg gcattcaaca caatggcttc gtgaatatat ttatcctccg gcaccatctg
     6841 gacctgacca tccaccagtt gccatgtcgc tttgcactgc gcagcaatat tatccatcac
     6901 gttgcggctg gatgagtaaa tcgcgcggcc acgaggaaac acggtatcag gaaaatcgcc
     6961 ggtaatgccc tgtgtcacgc cgaacgcgtt gaaatcctgc atcgtcgccc ggtgcagatc
     7021 cgcaacggta tatccagcgg caagcgtggt gatggtggtc gcatagagga acgcttcgtg
     7081 atcaccaatg gcctgaatca acacccagga atccgtaatg ttgtccttcc cggtgacggt
     7141 gaagcgaata tcaccgtcaa aaatcaggcc gtagttctga ccgttcacct gccctatctg
     7201 gtctggtgaa atctcccggg acacaccaac ctggctcgca tcaacatccg gcgctatacc
     7261 gtcatacccg gcaatgatgc gaattttggc aaactcctgc ccaagtatct tgttcgtggt
     7321 atcggtcgaa aggttgtaaa ttttcacgtt cgccacgcgc ggccagcgtg tgtctgccca
     7381 ctcgatctgg aacgtgacct taaagtcaga cagggaaacg ccctgcccgt tctggtccaa
     7441 cagttgcagc tcaaaatggc gcatccagtt aagagacatt tctactcctg tacgaaaatg
     7501 aggtggctgt atgtgccgag gttggttttg gtgggctcgt ctggtgcgcc cttatcggtc
     7561 gccaccacca gcgcgccatc aatgccaagc tgtggatatt gtcgtaaaag gtttacgccg
     7621 gtcagtagag gtacgccaga gagaagcgcg gcaccgccgc tatccatcac gtccataatc
     7681 cagccagccg catcacgcca gatgatcctg agtgtatacg tggtattgcc cagcaaaact
     7741 cggaactgct gattgtcagg agaaagcggt atttcgttaa actgcatatc atcccccgaa
     7801 tgctgatgta acgcttccgc ccagctggct cagcaaggat tcgtttggcg gtgtagtgga
     7861 tttcatcccg gaattctgca ccgccgatgt gctgacgcca tcctgcatat ctgatttatc
     7921 tgcaacgcta acgctctgcg tctgcgacat gatcacttca cgcagggtaa gcgtgcagtt
     7981 cagcacgttc tcgctggttt tatccgttgt cacctcgatg gctcgcacca gcatattgct
     8041 gtaaacccgc tttccggtca ctacatcgaa cggcacccta gaggactgga gatccagtag
     8101 ttgctgatag gtctctttcg ggctaagtcc ggcgctgagg ccgattgacg atgtatcaat
     8161 gaagtccagc aacgaaccgc cacctgcgaa gccgcattcc atcgtgactt cgctggggcg
     8221 cttatacgca tgatcggcga tgaaacccga cgcgctattc gttgttggct tctccaccgg
     8281 gtgctcagta atttcgagcg catcagaatg cttttcagaa acaaccacgc tggggattaa
     8341 tattccaatt cgccgggatt gctggcgaaa aattgctgat aaaatatcca ttatctcggt
     8401 cctgcgggga gttgctgggt taactgtgaa ttcacgccct tttgacggtc aacagtcaaa
     8461 cgggcagcct cgcgcggatc gaaaacgccg tggatgttaa tattcgtttc ctgctgaatc
     8521 accggggcgc tggtgggcat attgctcatt actttcggaa tgtagttgcg cgtttcctgc
     8581 ggcattagcc ccattccata acgcttaaca ttcccgatcc cccagttata tgatgccagt
     8641 gctttgctaa ggtctccgcc gttctgccgc aacagctggc tcaggtactt agcggctgcc
     8701 tgagctgact tttccgggtc gaatacatca ttcccacgca ggcccatgtc gcgcgccgtg
     8761 ccatccataa actgaaacag acctttagcg ccagcgcctg aaacggcgaa ctgattaccg
     8821 cccgattccg ttatggccac gctgcgcaat aacccctccg gaagccggta gagctgttcc
     8881 aggttggtaa acatcggctg catccatccc agcagttcag agcctgcttt ggttggctgt
     8941 ggccgcttaa ctgactggcc gagctgctca ggctcatcat caccgaacca gccgcgcacc
     9001 gttcggccca cgctgcgagg atcgaatccc cagtgctctt taatccagtc ggcggttccg
     9061 ttggcgctgt ctgttaccat cggcatcgct gacggatttt cgctgccctg attaagcatc
     9121 tgtttgccga tgctggcggc atcagcccag cggccatctt taatggcgtt gagcaggtcg
     9181 gcgatcatgt tcagcatctt gccgaactca cccatttgcg aaatgaagtt gctaaaatcc
     9241 cactttaggg accatgactt ggggtcaata ccgagcaact tagccagcgc ttttgtcagg
     9301 tccgtaacag tctttttcag gtcaccaatc attttgacgg cctgatcaat ttcagtcttc
     9361 catttccccc agtcaatcag gcttttgcca ccttctttcc acgtctggta atcatccaag
     9421 agcagaccaa gtgcaataat aagcgtcgtt atcatgccca agggtgatga catgaaagcg
     9481 gtatttagca gccgccatgc aaccaacagc ccactgaaca atgcgataag ctgctgcgtg
     9541 gctgggttca gctttttgaa ccaggcgatc acaccctcaa cagcttgccc agttcgccat
     9601 aacacgcggg taatcgcgtc acctgcccag agaattcctt tgatgatctt cgtgatgacc
     9661 gcttcaatct tcggccagtt gtcgagtatc tgcctgcgga agttatcaat actgccagca
     9721 aggccaccag ctaggttaga gccaatcttg tctttcgcct gtccaagcgt catcgtcaga
     9781 ttacgcatgg aggtcatgaa aatattggac tgtttagccg ctgactcagc attaaaacca
     9841 atacgctttg ccgtcaacgc gtactcagaa ctgagctgcc ccatccctct gcgcatcgcc
     9901 atcagcgtgt tttcatcgat gccgagcatc tgcgcgtatt gcttcgcgcg gtaatacggc
     9961 atgttgttga gcttttggcc aacgccagta aagatggcag cagtatcacg catctttccg
    10021 ctggcatcgc gggtctggac accaagacgg ttcaggaacc cttccgcccc cggattgcta
    10081 cgcatgaagc cagccaaccc ttcgagggag gacatagccg actcggcgct ggcaccggtt
    10141 tgcgatgcgg cgtatcccag cgctttgatg ccctggacac tggcccccgt ccgctgggat
    10201 gcccagtaaa ttttatccag accgttcgcg atctgagtgg taaatccgac aatgctcagc
    10261 gctgcgcctt tcaccaccgc gccgaccttc agaacgttcg cggtaacgcc tttcagcacg
    10321 gcttcaaact tattagcgcc agcctgatcg atatcgaatc ccagcgaaac aaggaaatct
    10381 ttaatcgtat ctgcgttacc gctcattggc cgctctccat ttatctaccc gggcgtcgtt
    10441 atcctcgcgc atgtcgaggt agtcattgag aagcgcgatg cggcaaagat ctaccgcacc
    10501 gctgttaagg tctttctggt caatatggaa ggcaagcgcc gggcgaagaa taaaatcttc
    10561 accgcccggc aggctgttga aggttattcc gctagcgggg tgggcgtctc gctggtaggg
    10621 agtccttgca aaaaatttcc cagcgagtcg gcgaccaccc gcgccaccag ttgcagcatg
    10681 gtaagcaggt cgatatcgtc aaacgccatt tcgccatgct ggcagaccgg cacccagcct
    10741 ttcatgtgct cgcgtgaaac aacggaaaga caggggaaca ggatagcgtc cacgtcgcca
    10801 tcactcagat cggacacagc attggcaatc tttggcagga tagtagccat cgcgccttcg
    10861 gtgtctttgc tgctgatctt ctcctgaacg ctccggaagt ccgaaaccat cccggccaga
    10921 accggcaaca gcttgcggga caccttcagc tgttcgaaaa cgctgagctt tgcggtgcga
    10981 tatttcacgc ctttaatttc gaattccatg cgttaaaact ccccgagaag ctggtcaatc
    11041 ttgccgcagt cgaataccca ggcgacggtt ccgccctctt tagcgttatt gaaatcaggc
    11101 tgtttctgga atgcacacga acgcgcagta gaaatatcac ccgatgccgt gttgcgaatg
    11161 acgatcacgt tattgcccca ggtggcagag gactggcttt gcgcgttata cgccagagac
    11221 agctttttat tcaccgggga ggttttgagt agcgtcaccg taatggtgcc tgacttatcg
    11281 gcgtgcaggc tgtgcatcac ctcgccatcg gcaccgatag tcatggtgtt tttgttgccg
    11341 cccatggtct gggtgatacc ttcctcagag ttcgcagaac cctgaccaag atcgataacg
    11401 ccggtcggcc cggtgagcga cgcggttaca tcgagaaaag aataagttgc catttatcgc
    11461 tccttagcga accacgttga tctgcacatc ggcataatga actgcgccag ccagcttaca
    11521 ggccacctgg attaacggtg ctttgcgcgc ttcacggtcg gcctgcgcct gttcggacag
    11581 cggttgcgca tacacgtaat agcctttggt cagcgtatcg ccggaattca gctgtccgat
    11641 agggccacca ttccacacgc cagcagctac cagaccgttc gtgacggact gatccatgga
    11701 ctgttcaacg ttggaaagca gacgggtcac accggcatca gtctgcggaa ttttggtggt
    11761 gctggtgtaa agcaggttat agaggttggt ctgaacgtag ttctgcaacc agtcgagccc
    11821 gtggcgctcg tcgaagaagt caccgttcgc catgacaccc tgttgcagga tcgccgtgtc
    11881 gttggcgtag tacacgaata cattgcagtt ttttgcatca agtgccgatg cctggctgac
    11941 tgtcagtgtt tcatacccga cacccggctc ctgcttaaac ttgagcgtaa tcgcggtatt
    12001 actgccattg aaattaaccg tgaatgcccg gccaaatgca gataacgcag cgtatttatt
    12061 acccgatgaa tattgaataa aactgcgtga atatccggcg gttttcagtt ttgatgccaa
    12121 atcatcgctg gatgcagtct gcaggcattt ctcatcgctt gtcgtaatcg ccagaatacg
    12181 gcttacagaa gaggattcga tcgccgcagc cactttcagc cagtctgcat ccggaatatc
    12241 ttcatcgtct gcaatcccca gcccatacca tgaagtataa tcgagcatgg cattcacagc
    12301 ctgctccagc gtctcaggcg tggcctgttc gctgtctccc ttcgttttca cccaacgacc
    12361 aacaaaaacc tcctgaggtt tcggtgattg tgagaaaaac acctgcgcag ccttatattc
    12421 tggtgattcc acgccaaaat cttttccaat atcttccgcg gcagaataac ggcgaatgcg
    12481 ctcacttacc ggaatgattg tggacgggcc gagaatgagt aatgcaccaa aatttcgccc
    12541 tgatgctgca cgcggcgaca tgatcacatc aacattaaca acgtttgata caggcaagcc
    12601 ctgtgccata gcttaatctc cgaaaaagat gactggtgct tccaccagcg atttaatacc
    12661 gtactcgcgc acaaccttcc ggcgcagacg caccgtcata tcgtagcggc ggacccattg
    12721 ctgattaata agttcaggga agagacctgt gtaatcgcca agagacagcc ccagcgcatt
    12781 cagtgctgcg ttgttctgcg gtacagatat accgtcacga aaccgggacg catacaccat
    12841 ccccgccgga ccataaaacg aagccataca ctcaatcgtt tcatgccgcc agagctgaga
    12901 gccatcatcg gtctgtctgg tgaatgccgg actgtcatca cctgaccatc cgataacccc
    12961 aaacgcacac cagttcgttt caaccggtag cagtggcggc tgctctttct gccagcgcgg
    13021 gcgaaccatc ccggcagaca gaccggaaac gttacgcatc cactggctta acagcctgtc
    13081 gagcgcttcg tcataatccg gatcgccact ggttggtatt aaccatccgc gctctgtact
    13141 ggtgttattg ctcaaccgga gttcccccat caaacggcat caactcacaa tgcgcctgaa
    13201 cgaatccggc cccataagct gtatacgggt cgacgaaggt cacacgataa tcacggccct
    13261 gatacgtcac gatatcggca tcacggccag tctgtccctg cgtcagtcgc tcagtcgtca
    13321 caatcagaat tgcaccgctg attacctgcc ctgcctgcat acggcggttt tccagagagc
    13381 gatcaacagt tacgactccg gcaaactgct ttttaacttc actgtcgctg ccgatcccgt
    13441 cctcatccac cgtttgcact cggcgtgtta cccacaaatt gaagtcgcaa aaatcggggt
    13501 caaaaagcac atctgttaca tcaagagtcg gcatctttat ccctcacaac atgggtaata
    13561 gctctgcgat attgcccggt gtcaattaat ggtttcgcca gatcggttcc gggagattcg
    13621 ccagcaacac gccgagcaag ttccagtgtt gcccccttgc gcccccgacg agcccgggct
    13681 tcaacggtgc tatcagcaag cggtgtaaag ccggtaatgg tcatgtaacg cctgacgcca
    13741 ttcgcggcca gcgttccggc gcggttaagc gctctttccg cacccgccgc atttccatca
    13801 agcgcagcct gcgccgctgc tttgagctgc ggcaccgtct gttcctctac ggatttaacg
    13861 ccggggatca ggtgcgggcg tgggggtatg ttttgcgctg gtgagccgta ttcgttgacg
    13921 taaccgatcc cggcattacc aaacggaaca tcctcacgct cgctgtcttc ttccgggatg
    13981 cccaccagca catccttttt ggttagcgac cggagcgcat ccagaatggc ctgagcgtta
    14041 tcaaccctcg ttgttacacc acttttgaaa ctcatagctg gcgaccgccc gcaccgaaca
    14101 tcgtgatcag ctgataaaat tcagcgccat atcgggtgtt attccagaag cctgcgtcag
    14161 ggtttagcgt cgcgctggtg tcatagctga cgcttacctt gtcaacggac ttggaggact
    14221 gaacaccatt ggttgaaccg cccgggccgc caaccagcat tgcccgacta tctgccgccc
    14281 atagcgtcat gtagtgagcc acgaacaacc cggcaaagta cggaaacaaa tctttgccgg
    14341 tgacgttttc gctcagcagt tcatcggcca gattcagacg aaactggatt tgcgcttcgg
    14401 gatatttggc agggtcagca aactgcggga agtcgcggcg aaaatcactt accgctggca
    14461 ggctttgatt ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat
    14521 acttccgcgt gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac tgcatgacgg
    14581 cctttaacaa actcgcgccg cgaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg
    14641 tgtattactg cattattttt tgccatcggg tcatccttaa tggccccgcc aggggggcca
    14701 tgtggctgtt aaatgccatc aacgtacgaa atggtttctt tgtacactgg ctcaaccgca
    14761 cccagcttgc cgtagtaggt cgcaatctga tacagaccgc gatactggat aggaacgctc
    14821 tgcaacggca ccagcggata gcggacgtat tttttatcgt tggtgtacgc aaccatgcga
    14881 tccttattcc ccacaccacg gcctttcagc catttaaccg cgcggatatt cagcggaaca
    14941 ccgttctggt gatagctgat ggtgttggtc tgaaggtacg tcaacaggga ctggttaccc
    15001 gcagatgaaa cgatgatgct ggacaacaga gcaaactgct ctggcgggat cagcaaatca
    15061 cgcggaacca cagagtaacc ggaagcggcc cacgcatcag acagcacctg gttaatgctt
    15121 gcgcggattt cgtccggtgt tgagattgcc cacgttttgg cagcgttgtt gacaggcacg
    15181 ccgtccaggg taacaaggcc tttcaggttt aatgcggaat cgccaacata cacctgctcg
    15241 tcgttgtcca tctgccattt gagctgcatc ccgtcgtact tctgggtgtc aatcgggcga
    15301 cctacctgct gagctgctgc cagctctaca acggtccagc ccagttccat gccccagaga
    15361 ttcagcgggt taccggattt gccgatatcc acgtttacgc cagcaatagc ggttgagtct
    15421 ttgcctaccc agtttttgcc attcggattt gcaccagtac ccgcagcggc gaagctggta
    15481 ttc
//
LOCUS       DAJTUN010000095        15227 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTUN010000095 DAJTUN010000000
VERSION     DAJTUN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.095"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO470_002853"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO470_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6252046.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO470_002854"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO470_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6252047.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO470_002855"
     CDS             complement(941..1408)
                     /locus_tag="OO470_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6252048.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1789..2100
                     /gene="rpsJ"
                     /locus_tag="OO470_002856"
     CDS             1789..2100
                     /gene="rpsJ"
                     /locus_tag="OO470_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6252049.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2133..2762
                     /gene="rplC"
                     /locus_tag="OO470_002857"
     CDS             2133..2762
                     /gene="rplC"
                     /locus_tag="OO470_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6252050.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2773..3378
                     /gene="rplD"
                     /locus_tag="OO470_002858"
     CDS             2773..3378
                     /gene="rplD"
                     /locus_tag="OO470_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6252051.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3375..3677
                     /gene="rplW"
                     /locus_tag="OO470_002859"
     CDS             3375..3677
                     /gene="rplW"
                     /locus_tag="OO470_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6252052.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3695..4516
                     /gene="rplB"
                     /locus_tag="OO470_002860"
     CDS             3695..4516
                     /gene="rplB"
                     /locus_tag="OO470_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6252053.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4533..4811
                     /gene="rpsS"
                     /locus_tag="OO470_002861"
     CDS             4533..4811
                     /gene="rpsS"
                     /locus_tag="OO470_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6252054.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4826..5158
                     /gene="rplV"
                     /locus_tag="OO470_002862"
     CDS             4826..5158
                     /gene="rplV"
                     /locus_tag="OO470_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6252055.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5176..5877
                     /gene="rpsC"
                     /locus_tag="OO470_002863"
     CDS             5176..5877
                     /gene="rpsC"
                     /locus_tag="OO470_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6252056.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5890..6300
                     /gene="rplP"
                     /locus_tag="OO470_002864"
     CDS             5890..6300
                     /gene="rplP"
                     /locus_tag="OO470_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6252057.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6300..6491
                     /gene="rpmC"
                     /locus_tag="OO470_002865"
     CDS             6300..6491
                     /gene="rpmC"
                     /locus_tag="OO470_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6252058.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6491..6745
                     /gene="rpsQ"
                     /locus_tag="OO470_002866"
     CDS             6491..6745
                     /gene="rpsQ"
                     /locus_tag="OO470_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6252059.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6910..7281
                     /gene="rplN"
                     /locus_tag="OO470_002867"
     CDS             6910..7281
                     /gene="rplN"
                     /locus_tag="OO470_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6252060.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7292..7606
                     /gene="rplX"
                     /locus_tag="OO470_002868"
     CDS             7292..7606
                     /gene="rplX"
                     /locus_tag="OO470_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6252061.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7621..8160
                     /gene="rplE"
                     /locus_tag="OO470_002869"
     CDS             7621..8160
                     /gene="rplE"
                     /locus_tag="OO470_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6252062.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8175..8480
                     /gene="rpsN"
                     /locus_tag="OO470_002870"
     CDS             8175..8480
                     /gene="rpsN"
                     /locus_tag="OO470_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6252063.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8514..8906
                     /gene="rpsH"
                     /locus_tag="OO470_002871"
     CDS             8514..8906
                     /gene="rpsH"
                     /locus_tag="OO470_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6252064.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8919..9452
                     /gene="rplF"
                     /locus_tag="OO470_002872"
     CDS             8919..9452
                     /gene="rplF"
                     /locus_tag="OO470_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6252065.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9462..9815
                     /gene="rplR"
                     /locus_tag="OO470_002873"
     CDS             9462..9815
                     /gene="rplR"
                     /locus_tag="OO470_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6252066.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9830..10333
                     /gene="rpsE"
                     /locus_tag="OO470_002874"
     CDS             9830..10333
                     /gene="rpsE"
                     /locus_tag="OO470_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6252067.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10337..10516
                     /gene="rpmD"
                     /locus_tag="OO470_002875"
     CDS             10337..10516
                     /gene="rpmD"
                     /locus_tag="OO470_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6252068.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10520..10954
                     /gene="rplO"
                     /locus_tag="OO470_002876"
     CDS             10520..10954
                     /gene="rplO"
                     /locus_tag="OO470_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6252069.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10962..12293
                     /gene="secY"
                     /locus_tag="OO470_002877"
     CDS             10962..12293
                     /gene="secY"
                     /locus_tag="OO470_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6252070.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12325..12441
                     /gene="rpmJ"
                     /locus_tag="OO470_002878"
     CDS             12325..12441
                     /gene="rpmJ"
                     /locus_tag="OO470_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6252071.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12588..12944
                     /gene="rpsM"
                     /locus_tag="OO470_002879"
     CDS             12588..12944
                     /gene="rpsM"
                     /locus_tag="OO470_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6252072.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12961..13350
                     /gene="rpsK"
                     /locus_tag="OO470_002880"
     CDS             12961..13350
                     /gene="rpsK"
                     /locus_tag="OO470_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6252073.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13384..14004
                     /gene="rpsD"
                     /locus_tag="OO470_002881"
     CDS             13384..14004
                     /gene="rpsD"
                     /locus_tag="OO470_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6252074.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14030..15019
                     /gene="rpoA"
                     /locus_tag="OO470_002882"
     CDS             14030..15019
                     /gene="rpoA"
                     /locus_tag="OO470_002882"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6252075.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15060..>15227
                     /gene="rplQ"
                     /locus_tag="OO470_002883"
     CDS             15060..>15227
                     /gene="rplQ"
                     /locus_tag="OO470_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="is a component of the macrolide binding site in the
                     peptidyl transferase center; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6252076.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAK"
BASE COUNT         3939 a         3569 c         4053 g         3666 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt actattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccaatcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agagcgctga tttgttaacc tgattccggt
     1501 gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt
     1561 tgcgattaat ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct
     1621 tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca
     1681 atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt
     1741 ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa
     1801 agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa
     1861 atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca
     1921 cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag
     1981 tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt
     2041 gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa
     2101 tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg
     2161 gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg
     2221 aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg
     2281 tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta
     2341 aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt
     2401 tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa
     2461 ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc
     2521 aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc
     2581 agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg
     2641 taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta
     2701 aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt
     2761 aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa
     2821 actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca
     2881 gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt
     2941 aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg
     3001 atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt
     3061 aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat
     3121 cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag
     3181 aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac
     3241 ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac
     3301 ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt
     3361 gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg
     3421 tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcga
     3481 aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg
     3541 aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc
     3601 gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg
     3661 ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac
     3721 atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc
     3781 ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat
     3841 caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg
     3901 caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc
     3961 gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg
     4021 cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac
     4081 cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg
     4141 taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg
     4201 tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg
     4261 tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg
     4321 tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga
     4381 ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg
     4441 cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg
     4501 tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt
     4561 ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc
     4621 ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg
     4681 ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca
     4741 aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga
     4801 agaagaaata aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc
     4861 ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc
     4921 tctggatatt ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga
     4981 atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac
     5041 gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg
     5101 tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga
     5161 ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta
     5221 aaaccatgga actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc
     5281 gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc
     5341 gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc
     5401 gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct
     5461 ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg
     5521 gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag
     5581 cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc
     5641 cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg
     5701 cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta
     5761 atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa
     5821 caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga
     5881 gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac
     5941 cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc
     6001 cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt
     6061 aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg
     6121 ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag
     6181 ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc
     6241 aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa
     6301 tgaaagcaaa agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc
     6361 tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc
     6421 acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg
     6481 cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat
     6541 ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt
     6601 catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga
     6661 cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg
     6721 cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca
     6781 gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc
     6841 tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg
     6901 agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt
     6961 cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac
     7021 atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg
     7081 ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt
     7141 cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg
     7201 cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct
     7261 ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga
     7321 agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc
     7381 ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc
     7441 ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt
     7501 agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg
     7561 caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg
     7621 atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt
     7681 aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt
     7741 ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc
     7801 tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt
     7861 cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt
     7921 gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag
     7981 tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa
     8041 atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg
     8101 aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa
     8161 ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt
     8221 tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg
     8281 cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca
     8341 gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga
     8401 agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc
     8461 tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca
     8521 tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag
     8581 ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag
     8641 aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc
     8701 tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc
     8761 tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag
     8821 ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg
     8881 gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac
     8941 cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag
     9001 gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata
     9061 ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg
     9121 cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc
     9181 agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg
     9241 gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc
     9301 agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc
     9361 tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg tgttcgttac gccgacgaag
     9421 tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc
     9481 gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg
     9541 tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag
     9601 ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta
     9661 acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca
     9721 tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg
     9781 cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat
     9841 cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac
     9901 cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg
     9961 tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat
    10021 ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc
    10081 tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg
    10141 tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct
    10201 ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct
    10261 ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat
    10321 tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg
    10381 tcgtctgccg aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac
    10441 cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt
    10501 taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa
    10561 ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg
    10621 tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca
    10681 gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac
    10741 agccgaagtt cgtctatctg acctggctaa agtagaaggc ggtgttgtag acctgaacac
    10801 gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg
    10861 cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc
    10921 tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat
    10981 tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta
    11041 tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg
    11101 ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt
    11161 tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt
    11221 cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga
    11281 aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc
    11341 tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag
    11401 gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag
    11461 gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta
    11521 tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta
    11581 tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag
    11641 tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa
    11701 actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc
    11761 tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc
    11821 cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt
    11881 cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct
    11941 tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga
    12001 agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg
    12061 ataaagtaat gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga
    12121 tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc
    12181 ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca
    12241 gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg
    12301 cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt
    12361 aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag
    12421 cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg
    12481 gctagattag ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa
    12541 cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag
    12601 caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg
    12661 tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga
    12721 tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg
    12781 ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt
    12841 gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca
    12901 acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata
    12961 atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg
    13021 gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac
    13081 gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg
    13141 tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag
    13201 aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg
    13261 aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt
    13321 tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag
    13381 aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta
    13441 ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc
    13501 cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa
    13561 aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca
    13621 gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac
    13681 aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc
    13741 cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg
    13801 aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg
    13861 gagctggctg agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa
    13921 ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg
    13981 atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc
    14041 tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc
    14101 caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg
    14161 ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt
    14221 actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa
    14281 cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa
    14341 atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt
    14401 caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat
    14461 caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga
    14521 tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc
    14581 ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga
    14641 aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct
    14701 ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga
    14761 agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt
    14821 ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg
    14881 taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa
    14941 agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc
    15001 aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca
    15061 tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt
    15121 tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag
    15181 cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaag
//
LOCUS       DAJTUN010000096        15178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTUN010000096 DAJTUN010000000
VERSION     DAJTUN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.096"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO470_002884"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO470_002884"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6252077.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO470_002885"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO470_002885"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6252078.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO470_002886"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO470_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6252079.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO470_002887"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO470_002887"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6252080.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO470_002888"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO470_002888"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6252081.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO470_002889"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO470_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6252082.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO470_002890"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO470_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6252083.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO470_002891"
     CDS             complement(8065..9207)
                     /locus_tag="OO470_002891"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6252084.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO470_002892"
     CDS             complement(9216..10229)
                     /locus_tag="OO470_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6252085.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO470_002893"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO470_002893"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6252086.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO470_002894"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO470_002894"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6252087.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO470_002895"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO470_002895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6252088.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO470_002896"
     CDS             complement(12827..13951)
                     /locus_tag="OO470_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6252089.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO470_002897"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO470_002897"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6252090.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4311 a         3416 c         3122 g         4329 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg gaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgc cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgctaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattt
//
LOCUS       DAJTUN010000097        15176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTUN010000097 DAJTUN010000000
VERSION     DAJTUN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.097"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..2022)
                     /gene="maeB"
                     /locus_tag="OO470_002898"
     CDS             complement(<1..2022)
                     /gene="maeB"
                     /locus_tag="OO470_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6252091.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAAL"
     gene            2311..3261
                     /gene="tal"
                     /locus_tag="OO470_002899"
     CDS             2311..3261
                     /gene="tal"
                     /locus_tag="OO470_002899"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6252092.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3281..5284
                     /gene="tkt"
                     /locus_tag="OO470_002900"
     CDS             3281..5284
                     /gene="tkt"
                     /locus_tag="OO470_002900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6252093.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5387..6430)
                     /locus_tag="OO470_002901"
     CDS             complement(5387..6430)
                     /locus_tag="OO470_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6252094.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6556..7131)
                     /gene="nudK"
                     /locus_tag="OO470_002902"
     CDS             complement(6556..7131)
                     /gene="nudK"
                     /locus_tag="OO470_002902"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6252095.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7199..9178)
                     /gene="aegA"
                     /locus_tag="OO470_002903"
     CDS             complement(7199..9178)
                     /gene="aegA"
                     /locus_tag="OO470_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6252096.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9384..11101
                     /gene="narQ"
                     /locus_tag="OO470_002904"
                     /pseudo
     CDS             9384..11101
                     /gene="narQ"
                     /locus_tag="OO470_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11303..14416
                     /gene="acrD"
                     /locus_tag="OO470_002905"
     CDS             11303..14416
                     /gene="acrD"
                     /locus_tag="OO470_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6252097.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14515..14574)
                     /gene="ypfM"
                     /locus_tag="OO470_002906"
     CDS             complement(14515..14574)
                     /gene="ypfM"
                     /locus_tag="OO470_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6252098.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            14955..>15176
                     /locus_tag="OO470_002907"
     CDS             14955..>15176
                     /locus_tag="OO470_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6252099.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKIT"
BASE COUNT         3595 a         3987 c         3932 g         3662 t
ORIGIN      
        1 cagcgctgca tcgccgtgca tttcaccatc aatcatcaga tctggcgcac gatccctgac
       61 cagttccagc gcctgacgca ttttgctcga cgacgggcag tcagaagaac caaagttgga
      121 gtgcgacaac agagcaacgc gcggctcaat accaaaacga cggacagttt ctgccgccat
      181 caaggtgatc tccgccagct cttctgcgtc tggttcgtca ttgacgtagg tatcggcaat
      241 aaaggtgtta ccactcggca gcagcagcgc gttcattgca cctgcggtgt gaacgccatc
      301 gcgataacca aagacatttt tcaccacgct aaaatgttca tgataatcgc ccaccgtacc
      361 gcagatcatt gcatcggctt ccccacgctg aaccatgatc gcgccgatca ctgtcgggtt
      421 actgatcagc gcacgctgcg cctgttcctg agtgacaccg cgacgcttca tgatctggaa
      481 gtattcggtc cagtactctt taaagcgcgg atcggattcg ttattgacga tttcaaaatc
      541 aacgcccgct ttgatctgca agcccagttt ctgaatgcgc atttcgatca cgttcggacg
      601 accgataagg atcggtttcg ccagtcccag cgttaccagt tcctgagtcg catgcagaac
      661 gcgcgcctct tccccttccg gcagaactac gcgcttcggc gctttgcgag cctgggagaa
      721 aatcggcttc ataaacaggt tggttttgta aacgaactca gtcagcttgt cgatgtagac
      781 gtcgaaatca gcaatcggac gcgtcgccac gcccgactcc atcgccgctt ttgcgaccgc
      841 aggagcgatc ttaacgatca agcgcggatc aaacggtttt ggaatgatgt attccggacc
      901 aaagctcaga tcctgaacgc catacgctga agccaccact tcactctgtt ctgcatgggc
      961 cagttctgca atcgcgcgta ccgccgccag tttcatctct tcgttgatgg cggttgcgcc
     1021 aacgtccagc gcgccacgga agatgaacgg gaagcaaaga acgttgttca cctggttcgg
     1081 gtagtcggaa cggccggtac agatgatggc atccggacgc acttctttcg ccagcggcgg
     1141 cagaatttcc ggttccgggt tcgccagcgc caggatcatt ggtgcccgag ccattttctt
     1201 caccatttcc tgggtcagca ctttcgggcc ggaacagccc aggaaaatat ccgcaccttc
     1261 aatcacatca tcgagggtac gtttgccgtc atccaccacc gcatatgcgg ctttggtttc
     1321 cgccatattt ggctcacggc cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt
     1381 atgtttttgc agccccagcg ctaccagcag gttcatacag gcgattgctg cggcacccgc
     1441 gccggaaacc accatccgca cgtcggagat gtttttctcc accacgcgca agccgttgag
     1501 gatggcggca gtgctgatga ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt
     1561 catccgctcg cgcagtttct gttcaatata gaaacattct ggcgctttaa tgtcttcgag
     1621 gttgatgccg ccgaaggtcg gttcgagcgc ggcgacgact tcaataaatt tgtccgggtc
     1681 gagttcatca acttcaatgt caaatacatc aatcccagcg aatttcttaa atagaacgcc
     1741 cttgccttcc atcaccggtt tgcctgccag cgcgccaatg ttgcctaacc ccagcaccgc
     1801 cgtaccgtta gagatcaccg caaccaggtt accgcgcgcg gtatatttgt aggcttttag
     1861 cgggtctttt tcgatttcaa gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag
     1921 atcgcgctgc gttgccagag gcttggttgg agaaacctgg attttccctg gaactggaaa
     1981 ttcatggaaa tcaagtgcac tttgttttaa ctggtcatcc atttggttgt tcctttcacg
     2041 taacgttcac aaataaagtg tgtgggcaac agcccctgcc cacaacgtgg cgcacattat
     2101 taccctgccg gagtctacag actttgagca agtccaaact ctcaccatta atataatgtt
     2161 ttggtaataa tcctataaca ctgatgttac ctgcttaatc cagcaatacc atgcctgtct
     2221 gctatgcttt tttgatgcgt ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc
     2281 taacacttta cttttcaagg agtatttcct atgaacgagt tagacggcat caaacagttc
     2341 accactgtcg tggcagacag ctgcgatatt gagtccattc gccattatca tccccaggat
     2401 gccaccacta atccttcgct gttactcaag gctgccggat tatcacaata tgagcattta
     2461 atagacgatg ctatcgcctg gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc
     2521 gcagcgtgtg acaaactggc ggtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt
     2581 tgcgtgtcaa cagaagttga tgcacgcctc tcttttgata aagaaaagag tattgagaag
     2641 gcgcgccatc tggtggactt gtatcagcaa caaggcgttg agaaatcacg cattttgatc
     2701 aaactggctt cgacctggga aggaattcgc gcggccgaag agctggaaaa agaaggtatt
     2761 aactgcaacc tgacgctgct gttttctttt acccaggcgc gggcctgtgc ggaagcaggc
     2821 gtttttctga tttcgccgtt tgtagggcgt atttatgact ggtatcaggc acgcaagccg
     2881 atggacccgt atgtggtgga agaagatccg ggcgttaaat cggtgcgcaa tatctacgac
     2941 tactataagc aacaccacta tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa
     3001 caaatcctcg ccttaaccgg ctgcgatcga ctgactatcg caccgaattt gctgaaggag
     3061 ctgcaggaaa aagtttctcc agtggtacgt aaattaatcc cgccttctca gacgttccca
     3121 cgcccagcac ccatgagcga agcggagttc cgttgggagc acaatcagga tgcgatggcg
     3181 gtagaaaaac tgtctgaagg cattcgtctg ttcgctgttg atcaacgcaa actggaagat
     3241 cttcttgccg ccaaactata aaccagccac ggagtgttat atgtcccgaa aagaccttgc
     3301 caatgcgatt cgcgcactca gtatggatgc ggtacaaaaa gccaattctg gtcatcccgg
     3361 cgcgccgatg ggcatggctg atattgccga agtgctgtgg aacgattttc ttaaacataa
     3421 tcctaccgac ccaacctggt atgatcgcga ccgctttatt ctttccaacg gtcacgcgtc
     3481 gatgctgctc tacagtttgc tacatctgac cggttacgac ctgccgctgg aagaactgaa
     3541 gaacttccgt cagttgcatt cgaaaacccc aggccacccg gagattggct atacgccagg
     3601 cgttgaaacc accaccggcc cgcttggaca aggtttggcg aacgccgtcg ggctggcgat
     3661 agcagagcgt acactggcgg cgcagtttaa ccagccagac catgagatcg tcgatcactt
     3721 cacctatgtg tttatgggcg acggctgcct gatggaaggt atttcccacg aagtctgttc
     3781 gctggcaggc acgctgggac taggcaagct gattggtttt tacgatcaca acggtatttc
     3841 catcgacggt gaaacagaag gctggtttac cgacgatacg gcaaaacgtt ttgaagccta
     3901 tcactggcat gtgatccatg aaatcgacgg tcacgatccg caggcggtga aggaagcgat
     3961 ccttgaagcg caaagcgtga aagataagcc gtcgctgatt atctgccgta cggtgattgg
     4021 ctttggttcg ccgaataaag caggtaagga agaggcgcac ggcgcaccac tgggggaaga
     4081 agaagtggcg ctggcacggc aaaaactggg ctggcaccat ccgccatttg agatccctaa
     4141 agagatttat cacgcctggg atgcccgtga aaaaggcgaa aaagcgcagc agagctggaa
     4201 tgagaagttt gccgcctata aaaaggctca tccgcaactg gcagaagagt ttccccgacg
     4261 gatgagcggt ggtttaccga aggactggga gaaaacgact cagaaatata tcaatgagtt
     4321 acaggcaaat ccggcgaaaa tcgctacccg taaggcttcg caaaatacgc ttaacgctta
     4381 cgggccgatg ctgcctgagt tgctcggcgg ttcggcggat ctggctccca gcaacctgac
     4441 catctggaaa ggttctgttt cgctgaagga agatccggcg ggcaactaca ttcactacgg
     4501 ggtgcgtgaa tttggcatga ccgctatcgc caacggcatc gcgcaccacg gcggctttgt
     4561 gccgtatacc gcgacgttcc tgatgtttgt tgaatacgcc cgtaacgccg cgcggatggc
     4621 ggcactgatg aaagcgcggc agattatggt ttatacccac gactcaattg gcctgggcga
     4681 agatggtccg acgcaccagg ctgttgagca actggccagc ctgcgcttaa cgccaaattt
     4741 cagcacctgg cgaccgtgcg atcagatgga agcggcggtg ggctggaagc tggcggttga
     4801 gcgccacaac ggaccgacgg cactgatcct ctcaaggcag aatctggccc aggtggaacg
     4861 tacgccggat caggttaaag agattgctcg tggcggttat gtgctgaaag acagcggcgg
     4921 taagccagat attattctga ttgccaccgg ttcagagatg gaaattaccc tgcaagcggc
     4981 agagaaatta gcaggagaag gtcgcaatgt acgcgtagtt tccctgccct cgaccgatat
     5041 tttcgacgcc caggatgagg aatatcggga gtcggtgttg ccttctaacg ttgcggctcg
     5101 cgtggcggtg gaagcaggta ttgccgatta ctggtacaag tatgttggtc tgaaaggggc
     5161 aattgtcggg atgacgggtt acggggaatc tgctccggcg gataagctgt tcccgttctt
     5221 tggctttacc gccgagaata ttgtggcaaa agcgcataag gtgctgggag tgaaaggtgc
     5281 ctgatggtga tcgccggatg ctgtatgttc ccctcaccct aaccctctcc ccagaagggc
     5341 gaggggacgg tccgtgcgct ttttagggcc gcatctgcga ttttgtttaa cgggtgatcc
     5401 acaacgtggg cctggcgtct ggcccatgcc agttatcgca ggtaggttct gcggcgtaac
     5461 gcaccaggcg aaaacgctga ccgtcaaagc gccagcgcgc ctgaatgcca caatcgctta
     5521 atccgcgccc tttcgctaag gtcaccagtt cacgcgattt ctcatcaaat gttgcgttca
     5581 tcagttccag ttcattcgtc tcctggccgt tgttgaacgg caaacgcaac cgaaccgggc
     5641 gcgaggctag cggcttttta cgcgacacaa tccatgccaa atcaatggtg ttataggccc
     5701 ctgcctcaca gctaatcatc atcagtgctt tatcatcggt cagcgcagtg acattcacct
     5761 cacgacgtaa cggatcaagc gagcagcgca gaccattcat ccgccagttt ccataatcca
     5821 gcaaatcgtt acgttcttcg agtgagagtg gcgtcggcgt tgggttaacc accgcgacct
     5881 ctttcagcgc aggcgcgggc ggtacgctga gaggcggttc gtcccctttc ttgatccacg
     5941 cggtttcact gccaacgcgc ttttgctgag catcaataaa caacaacgcc gcttttaagc
     6001 cgctcagaga aatggtctga ttgccgtcgc gtaaggtgat cgccttccct tcctgaatta
     6061 tctgcaaaaa cgcggtgatg gttgccgtat catcggttac taataaccat ggtgaaatcc
     6121 gccacttgtc accacttaat gccaacggct caccatctaa tagcagccgt ggcgctatct
     6181 ccccttcaga cgcatccggc gacttcaacc caccgcgctc aatacgtaaa acggcatcgg
     6241 tatgcgcccc agcgctgcgg ctcagggtca tcaccagtcc attatgatcg cccgtattgc
     6301 gcgccacaca aaaattttga ttattacagg tgacctgcca gtcggaaaac gcccgttgcg
     6361 ctggtgccgc ccacaccaaa gacgtcggca acaaagcgaa aaaaaagaga aggaaaatgc
     6421 gatagcgcat ggacggtacg accccagaag ttcaaacaaa cagcacaagt cgtatcttcg
     6481 ttgcgcaggt agcgcggctc aatcggattt gtcggattga gccaataata aacaacgaaa
     6541 tattattgct atttttcagt ccattaaatg tgacatttgc aaatagttaa gcaataacac
     6601 cgtcttaccg tcacgtatct cgccggtttt gatcatctcc agcgcctggc tgaacggcag
     6661 ctcgagcact tcaatatctt catcttcaac accgccaccg gcgttagcgc gctgattgtc
     6721 actgtattcg gcgataaaaa agtggattag ctcagtcaca ccgccaggcg acatatacag
     6781 ttcaaataat ttgcgcactt cgcccacttc atagcccgtc tcttcaatcg cttctttacg
     6841 aatgcacact tccggttcgt cgttatccag cagcccggcg caggtttcaa tcagctgccc
     6901 gctttcattg ccattaaccc aggtagcgac acggaactga cgaatcagaa ccacgctctt
     6961 tttcttcgcg ttgtacagga ggatcgtcgc accattaccg cgatcgtaaa cttcacgttt
     7021 atggcggata acttcgccat ctttgcgcgt gagatcgtaa gtaatgttgt gcagggtgaa
     7081 atagttatcg gagagaattt tgtctttaat gagggtgatt tgttgcgtca taccgactcc
     7141 acagcgcgaa atgaacaatt atattacgct gtgaagtcgg gtttgtctgc gcaggctatc
     7201 agtgagattt gactgatttt acccccagcc agtcaataat cccctgtgcc gcatgacgtc
     7261 cttctgccat tgcggtaacc accagatccg caccacgcac ggcgtcgcca ccggcgaaga
     7321 ttttcggatt ggtggtctgg taacggtact ggctttccac atccgcgatg atgcggcccc
     7381 atttgtctac cgttacaccg tgcgactcca gccacggcat cccgtgtgga ttgaagccaa
     7441 acgccataat caccgcatcg gctggcatga caaattcact gccctcaatc agtacaggtc
     7501 gccgacgccc ctgggcatcc ggctcgccaa gacgcgtgcg caggaaacga attccgcaaa
     7561 cgtgaccttg ctcattcagc tcaagcgcca ccggctggac gttaaattcg aagttggccc
     7621 cctcttcgcg ggcgttcttc acctctttct tcgagcctgg catgttagct tcatcacgac
     7681 gataagcgca ggtgacgtta ctcgcgccgt ggcgcagcgc agtacgcaca cagtccatcg
     7741 cggtgtcgcc gccgcccagt accacgacgt taagtccggc ggtattgata aacggctctt
     7801 ccggtagctc ttcgagcccc atcacctgtt tagtgttggc aatgaggaac ggcagcgcgt
     7861 cataaacgcc cggcgcatct tcattgggta aacccgcttt catggaacgg taagtgccta
     7921 cgccaacgaa gaccgcgtcg tattgttcca aaagcaaatc caaagagaca tctttaccca
     7981 cttcacaatt gagttcgaag tgaatcccca tcgcgctgaa gatttcccga cggcgtgcca
     8041 gcagggattt atccagtttg aaagaaggaa tgccgaaagt gagcaagcca ccgatttctg
     8101 gatggcgatc gtacaccgtc accgccacgc cattgcgggt cagaacatcc gcacaggcca
     8161 gccctgccgg acctgccccg ataatcgcca cccgcttgtc cactttggtg acatgactta
     8221 agtcaggacg ccaacctttc gccaacgcct gatctgaaat atagcgttca atgttgccga
     8281 tagttaccgc gccgtgctca tcgcgaatag tacaggcacc ttcacacaaa cggtcttgcg
     8341 gacaaacgcg tccggtaatt tccggcaggg tgttggtctg gtgagaaagc tcgacggcgg
     8401 cgtcgatgtt tccggctttc accagttcaa tccactgcgg aatatggtta tgcagcgggc
     8461 aggtccattc acaaacgctg tgctcgccga acttaaggca gcgcgaggct tcccgttgtg
     8521 cctggtcggc gcgaaatggc agataaattt catcaaaacc ggttttgcgc gcttcaatcg
     8581 ccagtttatc cggctcgcca cgcgcgggcg ttgcctgcat ttgttcgact ttactcatta
     8641 ccggcatttc ttgtgcagcg gtactggcat gccacggttg atgctcctga cgtgcggtgc
     8701 gcaagcggcg ggatttcgcc atgccggaga gtgcgacgtc agtgaccagt tgcagcgcgt
     8761 ccgccgggca attctcgaca caggcaggac cgttttcgcg ccctgcacaa aggtcgcatt
     8821 tatgcgccgt ggcttttact tttcctgccg cgacgggtgt caggacgatt tgcatcgtac
     8881 caaacggaca ggccaccacg caggatttac agccaataca cttttgctga ttgacctgaa
     8941 tgctgtcatc aacgtggctg attgcgccat tagggcagct acgggcgcag ggcgcatctt
     9001 cacaatggtg acaggtcact gcactacgtt gctgctgatg tttgataacc gtaattcggg
     9061 gatgaaaatg gtgttggctc aggacatgtt gctcatcatt gtgagccatg acacaggcga
     9121 tttcacaagc atgacaaccc agacattgct gactgttggc cataataaaa cgattcataa
     9181 cgaccttctt ttttggttgt aaaaacctta ttctttatat gagtgttgtt attacccgac
     9241 ttacagggga accggcaatg ttcatgtgcc cagaataagt aactatttcg ctataaactg
     9301 tggcagatca aataatccca tcactgacta aattgcgttt cagcgaactg gaacattaat
     9361 gattttttgt ggagaagacg cgtgtgattg ttaaacgacc cgtctcggcc agtctggccc
     9421 gggccttttt ttacattgtg ctgctgtcga ttctttccac gggtatcgct ctgctaactc
     9481 tggcgagcag tttgcgcgac gctgaggcta tcaatattgc cggatcgctg cgtatgcaga
     9541 gttaccgcct gggctacgac ttgcaaagtg gcagtccaca actcaatgca catcgccagt
     9601 tgtttcagca ggcactgcat tcaccggtat taaccaacct caacgtctgg tatgtgccag
     9661 aagcagtaaa aactcgctat gcgcatctga atgccaactg gctggagatg aataatcggc
     9721 tcagcaaggg cgatttgccg tggtatcagg ccaatattaa taattatgtt aatcagatag
     9781 acctgttcgt gctggcttta cagcactacg ctgaacgcaa aatgctgctg gtggtggcga
     9841 tttccctggc tggcggtatc ggtattttca cgctggtctt ttttactttg cgccgcatac
     9901 gccatcaggt ggttgccccg ctgaatcagc tggttaccgc cagtcagcgt attgaacacg
     9961 ggcagttcga ctcgccgccg ctggatacca gtctgccgaa tgagcttggt ctgcttgcaa
    10021 aaacctttaa ccaaatgtcg agcgagctgc ataaattgta cctttcgctg gaggcgtcag
    10081 tagaagaaaa gacccgcgat ctccacgaga ccaagcgtcg tctggaggtg ttgtatcagt
    10141 gcagtgttcc caggcgctga acaccagcca gattgatgtg cattgtttcc gccatatttt
    10201 gcagattgtt cgcgacaatg aagcagctga atatctggag ctgaacgttg gggataactg
    10261 gcggattagc gaagggaagc caaaccctga gctgccaatg cagattttac cggtgacaat
    10321 gcaagagacg gtttacggcg aactgcactg gcaaaatagt cacgtttcat catcagaacc
    10381 gctgcttaac agtgtttcat cgatgctggg acgcggtttg tactttaatc aggcgcagaa
    10441 gcattttcag cagttattgt tgatggaaga acgtgcgacc atcgcccgcg aattgcacga
    10501 ctcgctggct caggtgcttt cttacttacg tatccagttg acgttactga agcgttcgat
    10561 accggaagat aacgccaccg cacaaagtat catggccgat tttttccagg cgttgaatga
    10621 tgcttatcgg cagttacgcg agctgttgac tactttccgc ctgacgctgc agcaggcgga
    10681 tctcccctcc gcgttgaggg aaatgctgga tacgttacaa aatcaaacca gcgctaaact
    10741 gaccctcgac tgccgtctgc caaccctggc actggatgcg caaatgcagg tgcatttgtt
    10801 gcaaattatt cgcgaagcgg tgctgaatgc gatgaagcac gccaacgcca gcgaaatcgc
    10861 cgtcagttgc gtcaccgcgc cggacggcaa tcacacggtt tatatccgtg ataacgggat
    10921 tggtatcggt gaaccgaaag aacccgaagg tcattatgga ctgaatatca tgcgcgaacg
    10981 cgcggaacgg ctgggcggga cgctgacttt ttcacaacct tccggcggcg gcacgttagt
    11041 gagtattagc tttcgctccg cagagggtga ggggaagggg aacacgccgg ctgaggtgta
    11101 aataattacc ctcacaccgg ccctctccct ttaaaggcga gagggaaaaa cgtgcccata
    11161 ttgacctgta agcgagtgtg tcagcaatct catgataatt tacattaact cctttttttc
    11221 tccacgattg gctcgtacct tgccgctaca gtgaagcaag tcaagcctac aacaatacgc
    11281 agaaacacga ggtcctcttt taatggcgaa tttctttatt gatcgcccca tttttgcctg
    11341 ggtgctggca atcctgttgt gtctgacagg taccctggcg attttttcat tgcccgttga
    11401 acaatacccc gatctcgcgc caccgaatgt gcgagtgacc gctaactatc ccggcgcatc
    11461 ggcccagacg ctggaaaaca ccgtgaccca ggttatcgag caaaatatga ccggcctcga
    11521 taatctgatg tatatgtcat ctcagagcag tggcaccggt caggcatctg tcactttaag
    11581 ttttaaagca ggcaccgatc cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc
    11641 agccatgcga aagttaccgc aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg
    11701 cgataccaac attctgacca ttgccttcgt ctctaccgat ggctcgatgg ataaacagga
    11761 tattgctgat tatgttgcca gtaatattca ggacccgtta agccgcgtga atggcgtcgg
    11821 ggatatcgat gcctatggtt cgcaatattc catgcgtatc tggctggacc cggcgaaact
    11881 caacagtttc cagatgacgg ctaaagatgt cactgatgcc attgagtcac agaacgcgca
    11941 gattgcggtt gggcaacttg gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac
    12001 cattaacgcc cagtcactgc tgcaaacacc agaacagttc cgcgatatca ccttgcgggt
    12061 gaatcaggac ggctcagagg taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga
    12121 gaaatacgat tatcttagcc gcttcaatgg taagccagcc tccgggttgg gggtaaaact
    12181 ggcctccagc gcaaacgaaa tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct
    12241 ggcgcagtat ttcccgcacg gactggaata caaggtggcg tatgaaacca cctcgtttgt
    12301 taaagcctcc attgaagacg tggtgaaaac gctgctggaa gctatcgctc tggttttcct
    12361 cgttatgtat ctgttcctgc aaaacttccg cgccacgctg atacccacta tcgccgtgcc
    12421 ggtggtgttg atgggaacct tctccgtact ttacgccttc ggttacagcg tcaacacctt
    12481 aaccatgttc gcgatggtgc tggcgatcgg tctgctggtg gatgacgcca tcgtggtggt
    12541 ggaaaacgtc gaacgtatta tgagtgagga aggactcact cctcgcgaag ccacacgtaa
    12601 atcgatgggg cagatccagg gggcactggt cgggattgcg atggtgcttt cggcggtatt
    12661 tgtaccaatg gccttcttcg gcggcaccac cggtgccatc tatcgccagt tctctattac
    12721 cattgttgcg gcgatggtgc tgtcagtact ggtagcgatg ctcctcactc cggctctgtg
    12781 tgccacgcta cttaagccac tgaaaaaagg tgagcatcac gggcaaaaag gtttttttgc
    12841 ctggtttaac cagatgttta accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat
    12901 tctccaccgt agcctgcgct ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt
    12961 cctgttcctg cgtttgccga cgtcgttctt accgctggaa gaccggggca tgtttactac
    13021 ctcggtacaa ttgcccagcg gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat
    13081 cgagaaatat tacttcaccc atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg
    13141 ttctggccct gggggtaacg ggcaaaacgt ggcgagaatg tttatccgcc tgaaagactg
    13201 gagcgaacgc gacagtaaga ccggcacctc gtttgccatt atcgagcgtg caacgaaggc
    13261 gtttaaccaa attaaagaag ctcgcgttat cgccagcagc ccaccagcaa ttagcggtct
    13321 tggtagttct gcaggttttg atatggagtt gcaggaccac gctggagcgg gtcacgatgc
    13381 gctgatggca gcacgtaatc agttgctggc gctggcggcg gaaaacccgg agctaacccg
    13441 tgtgcgccat aacggcctcg atgacagtcc gcagttgcag attgatatcg accagcgtaa
    13501 agctcaggcg ctgggcgttg atatcgacga tattaacgac acactgcaaa ccgcctgggg
    13561 ttcgagctat gtgaatgact ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc
    13621 agctgcgccg tatcgcatgc tgccagatga catcaatctc tggtatgtcc gaaataaaga
    13681 tggcggcatg gtgcccttct ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg
    13741 tctggaacgc tataacggtt attctgcggt tgagattgtt ggggaagccg caccgggggt
    13801 cagtaccggt acggcgatgg atattatgga atcgttagtg aagcagctgc caaacggctt
    13861 tggtctggag tggacggcga tgtcgtatca ggagcggctt tccggcgcgc aggctccggc
    13921 gctgtacgcc atttccttgc tggtggtatt cctgtgtctg gcggcattgt atgaaagctg
    13981 gtcggtaccg ttctcggtaa tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc
    14041 aacctggatg cgcgggctgg aaaacgacgt ttacttccag gtgggcctgt taacggtcat
    14101 tggtttatcg gcgaaaaacg ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa
    14161 aggccatgac ctgtttgaag cgacgctcca cgcctgccgt cagcgtttac gcccgattct
    14221 gatgacctcg ctggcattta tcttcggcgt attgccaatg gcaaccagca cgggtgccgg
    14281 ttccggtggt cagcatgcgg tgggtactgg cgtaatgggc gggatgattt cagctactat
    14341 tctggctatt tacttcgtgc cgctgttctt tgtgctggtg cgccgccgct tcccgctgaa
    14401 gccgcgcccg gaataagcaa taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt
    14461 tccgattaag aacctgctca gcgggttctt gctgtttata ctttgtctca ggaattactt
    14521 acgaagcata acttcgataa agtctttcca gttccccagt tcacgttcaa tcataacaac
    14581 ctctcttata attatgggta ttctacggaa acaatatacc gtggtgaagc taatttactc
    14641 gattgctgcg atgactacct ccgggggaca aaccttatgt aaatactatg gtcctgcggt
    14701 gataatttgt atgtgataca cagcaacatt tcgagatatt catacggcat ctaatactga
    14761 tttaattctg gttaaaatac agacagataa caagatgaat attcttaatg tttacgttaa
    14821 aaatgtttaa tattatttaa tagttgttaa tttgaatact tcgataatgt tatattttct
    14881 gataatcatt tgcaggcaaa atgttttcac ccttaaatga gtatttattc tcataaatcg
    14941 aaaaaggatt cattatggtt acactttacg gcatcaaaaa ttgtgacacc attaaaaagg
    15001 ctcgccgttg gctggaagcc aataacatcg actatcgttt tcatgattac cgcgtcgatg
    15061 ggctggacag cgaattattg aacggtttta tcaacgaatt aggctgggaa gcgttactca
    15121 acacccgtgg tacaacctgg cgtaaactgg acgaaactac ccgcaataaa atcacc
//
LOCUS       DAJTUN010000098        15040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTUN010000098 DAJTUN010000000
VERSION     DAJTUN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.098"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..166)
                     /locus_tag="OO470_002908"
     CDS             complement(<1..166)
                     /locus_tag="OO470_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252100.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNEL"
     gene            complement(582..1523)
                     /gene="fabY"
                     /locus_tag="OO470_002909"
                     /gene_synonym="yiiD"
     CDS             complement(582..1523)
                     /gene="fabY"
                     /locus_tag="OO470_002909"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6252101.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1568..2005)
                     /gene="dtd"
                     /locus_tag="OO470_002910"
     CDS             complement(1568..2005)
                     /gene="dtd"
                     /locus_tag="OO470_002910"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6252102.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2002..2874)
                     /locus_tag="OO470_002911"
     CDS             complement(2002..2874)
                     /locus_tag="OO470_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6252103.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2868..3467)
                     /gene="yihX"
                     /locus_tag="OO470_002912"
     CDS             complement(2868..3467)
                     /gene="yihX"
                     /locus_tag="OO470_002912"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6252104.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3566..4351)
                     /locus_tag="OO470_002913"
     CDS             complement(3566..4351)
                     /locus_tag="OO470_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6252105.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4385..5281)
                     /gene="yihV"
                     /locus_tag="OO470_002914"
     CDS             complement(4385..5281)
                     /gene="yihV"
                     /locus_tag="OO470_002914"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6252106.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5449..6345
                     /gene="yihU"
                     /locus_tag="OO470_002915"
     CDS             5449..6345
                     /gene="yihU"
                     /locus_tag="OO470_002915"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6252107.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6369..7247
                     /gene="yihT"
                     /locus_tag="OO470_002916"
     CDS             6369..7247
                     /gene="yihT"
                     /locus_tag="OO470_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6252108.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7264..8505
                     /gene="yihS"
                     /locus_tag="OO470_002917"
     CDS             7264..8505
                     /gene="yihS"
                     /locus_tag="OO470_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6252109.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8643..9545
                     /locus_tag="OO470_002918"
                     /pseudo
     CDS             8643..9545
                     /locus_tag="OO470_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9744..11780
                     /gene="yihQ"
                     /locus_tag="OO470_002919"
     CDS             9744..11780
                     /gene="yihQ"
                     /locus_tag="OO470_002919"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6252110.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11826..13200
                     /locus_tag="OO470_002920"
                     /pseudo
     CDS             11826..13200
                     /locus_tag="OO470_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13243..14646
                     /locus_tag="OO470_002921"
     CDS             13243..14646
                     /locus_tag="OO470_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6252111.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14714..>15040
                     /gene="ompL"
                     /locus_tag="OO470_002922"
     CDS             14714..>15040
                     /gene="ompL"
                     /locus_tag="OO470_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6252112.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3695 a         3871 c         3743 g         3731 t
ORIGIN      
        1 tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg caaagtgaaa
       61 attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt tctaaattaa
      121 caacagaata actttcttgt ttaatgagat taatattggc aaccattata gcctccttcc
      181 cacggtttaa gagcaaacat acaacataat gattcataac atacagagca gtaaatgaat
      241 gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc tatagatttt
      301 aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa aaatcgctca
      361 aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc agctctacaa
      421 ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc caacgaagaa
      481 taaaaattta taaagttctt attcaatata accggaatag tcagggactg atgtaaatga
      541 ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc ttcgttcccg
      601 ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc ttcaaacact
      661 gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc ttttcgtccg
      721 cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc atggggttta
      781 ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt tccgccgagg
      841 tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc cagcgagaat
      901 aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg catggtggtg
      961 ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact aagcgggata
     1021 tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa aatatcatcc
     1081 agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt tggcgtggtg
     1141 atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc ttcacgggcg
     1201 ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt catcgccatc
     1261 agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg aatggacgct
     1321 tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg ctcgtcgacg
     1381 accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt tggttgatgc
     1441 aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc ttcttctgtt
     1501 tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga caaccctgga
     1561 agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc gatacctgca
     1621 tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag cgttcgacga
     1681 aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa cttgggcgca
     1741 tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca ctgccgcccg
     1801 cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg tagccgagca
     1861 cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca cccaataaca
     1921 ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg ctggcacggg
     1981 ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag ctgcttgttt
     2041 tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga cgatacacca
     2101 tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt aaatgagctg
     2161 atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt cgaacaggag
     2221 tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg taggaacaat
     2281 gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac gcagcacgtt
     2341 gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg aactgatcgc
     2401 cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca cggcgaacga
     2461 gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc tatcgatgga
     2521 gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg tcatcttgtt
     2581 ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag caggcagaaa
     2641 gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg gaaaagcggc
     2701 gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca cataggcaag
     2761 gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta gttttagcca
     2821 ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta gcataacacc
     2881 ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt aatgcctagc
     2941 tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc gctgggtgaa
     3001 aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg catccccaga
     3061 tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc ttccggccag
     3121 aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc ctgctcacgc
     3181 agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc ctgccagccg
     3241 tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc ctctgcgaac
     3301 gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat atgaaaactc
     3361 ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag cacacggtta
     3421 aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt tgcctccttt
     3481 cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg gcaaggtatg
     3541 aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg atttcaattt
     3601 ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta aagagcgcag
     3661 tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta gtgtgatcgg
     3721 cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta gcttcgttta
     3781 catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt accagataat
     3841 cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg ctattatgag
     3901 agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa agaatatgcg
     3961 ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac gcccgggcaa
     4021 catgctcaac agtcgtacca atggtgataa atattgttga accatcagga atatagtctg
     4081 ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc tgctcgaacg
     4141 ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta atcaggcctt
     4201 gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc agcagatttg
     4261 ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt tggtcgtgcc
     4321 gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc atcaccctgg
     4381 cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg agcccggctc
     4441 gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg actgactccg
     4501 ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca tcacccgcac
     4561 ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca ccattttcca
     4621 gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg agcgtttgtg
     4681 cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga cccggttctg
     4741 aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc gtaatgtccc
     4801 cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt ttagcgccgt
     4861 cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt tcctccaacc
     4921 actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc tcgcctttgg
     4981 tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg gtgtaacggg
     5041 tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg tcatcaccta
     5101 cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct gccgtcgccg
     5161 ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc tccgtcggta
     5221 acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca acacgaatca
     5281 tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt gtttaaaaag
     5341 tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag acaagcaaag
     5401 catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat ggcagcaatc
     5461 gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt gcagcaaggg
     5521 catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt agacaaaggt
     5581 gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat cattaccatg
     5641 ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt ttgcgaaggc
     5701 ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca aaccgataaa
     5761 ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt aggccgtact
     5821 tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc tgaacaagtt
     5881 gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc tggcggtccg
     5941 ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct caatgcgctt
     6001 tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt tgccgtcaaa
     6061 gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc aaacaaagtc
     6121 ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga tcttggcatc
     6181 gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc ctcacgggag
     6241 gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc cattctggaa
     6301 caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact ggataaagga
     6361 ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc ggttttgcca
     6421 tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct ggggcacccg
     6481 ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag gccctctcgc
     6541 cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg gttgagcaaa
     6601 acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt cctggcaacg
     6661 gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag atcaaacaag
     6721 acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat gcgcagcaac
     6781 gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg gtaagcatca
     6841 ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa caagcgatca
     6901 tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt gaaatgccgc
     6961 tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg aatgaccata
     7021 tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc ccgcgtgccg
     7081 tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca gtctgggcat
     7141 cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca ccgaaattac
     7201 aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat gaggataaaa
     7261 ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga aaccgaccgc
     7321 atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt aggcaataaa
     7381 gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat gttgcacgtt
     7441 tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga tcacggtatt
     7501 aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc ctgcgtgaat
     7561 gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc tctgctgggt
     7621 gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga ttacaccatt
     7681 gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga gtcctgggac
     7741 gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca cgcggtggaa
     7801 gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg cgcgattcgc
     7861 gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt taacgaacac
     7921 ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc gcatcgcttc
     7981 cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat gctgcacatc
     8041 catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga agatgccaaa
     8101 ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga cgggattgtt
     8161 tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg gcctatcgtc
     8221 gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca gtatgaaacc
     8281 tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga aaatggttcc
     8341 tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga cggcaaacag
     8401 gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc gcccggcatg
     8461 gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat ctgaaaatag
     8521 ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccggtg
     8581 cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt aaggtgcctt
     8641 gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc gcgggggatt
     8701 atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt cagggttgtc
     8761 atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt aaagtgctta
     8821 ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag gaatatcagt
     8881 tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc tggcgagact
     8941 ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg ccgcccagtt
     9001 acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca cacacgggtc
     9061 tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat ggcgtgggta
     9121 ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc cagcttccgg
     9181 caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac cacgttgatg
     9241 agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc gaccacacct
     9301 ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag gcgctttcgg
     9361 taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa ttacagcgtc
     9421 agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca ggtatcgact
     9481 tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac ggacagagaa
     9541 aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg ctaaaggcaa
     9601 cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa gtttttatat
     9661 atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca acaatctcgt
     9721 tctcaactct atggagatta gttatggata cgccacgtcc acagttatta gattttcaat
     9781 ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt ttaacccata
     9841 acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat atgttccgcg
     9901 gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac gccatcgtca
     9961 gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt agcgccacgc
    10021 tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac gacaacctta
    10081 accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc tacggctgcg
    10141 gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg accagtgaac
    10201 aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc aaagaaaatg
    10261 cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc acgcagaagt
    10321 attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc ccggaatacc
    10381 atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct gacacataca
    10441 tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg cccgactgga
    10501 tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag aagaaactgg
    10561 acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac tggtctggga
    10621 tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac agcgaaaact
    10681 acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag ttcctggcct
    10741 atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca caacacggct
    10801 atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag ttttacggcg
    10861 gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg atcaaaaaga
    10921 acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat ctgcccaccg
    10981 acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg cctgcgctgt
    11041 gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag atcctcttct
    11101 ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg gcgggcgacc
    11161 agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg gcgctgtcgc
    11221 tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc accctgtttg
    11281 agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc ttcacgccga
    11341 tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac ggcgatgcgg
    11401 aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa ccttatctga
    11461 aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg ctgttcctgc
    11521 attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta ggccgcgaca
    11581 ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat ctgccggagg
    11641 ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt accgttaatg
    11701 cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg gcactgttcg
    11761 cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc aaagggagaa
    11821 catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc tttaaagaga
    11881 aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc ggtacgcttt
    11941 atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc ggcattatct
    12001 ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt atgttggatt
    12061 cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat gcgtcattcc
    12121 cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc accggtaaaa
    12181 cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg atgaactgct
    12241 cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc tcactggcgg
    12301 catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga ttcgtgccag
    12361 ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc acgctgtttt
    12421 cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag cgttatgtcg
    12481 aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc cgcgcaattg
    12541 ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta ggggcgttta
    12601 acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat cccatcctgt
    12661 tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc ctgatgcctg
    12721 gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg atttgggtgc
    12781 tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg gcgttctcct
    12841 gcctggcgtt cttcggctca gcgcctgaac tgggcgctgg tttccgacac cgtcgagtac
    12901 ggcgagtggc gcaccggcgt gcgttcggaa ggcacggtct acaccggctt caccttcttt
    12961 cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac gcaaattggc
    13021 tatgtgccga acgtcgcaca ggctgaccac accattgaag ggttacgcca gttgatcttc
    13081 atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggttgctt ctacagcctg
    13141 aacgagaaga tgtatgtccg cattgtggaa gagatagaag cccgtaaacg cacggcgtaa
    13201 taataataaa cgacgccctg cggggcgtta taaggagtga ttatgtctga ccataatcca
    13261 ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga cgtcggttcg
    13321 aatttaatgc tctgcatcgg tactctgtat ctcctcaaac tttataccga tgaattaggg
    13381 atgcctgctt actatggcgg gattatcttt ctggtcgcga agttttttac cgcgtttacc
    13441 gatatgctca ccggattttt actcgactcg cggaaaaata ttgggcctaa aggcaaattt
    13501 cgtcccttta ttttatatgc agcagttccg gcggcgttaa ttgcaacgct ccagtttatc
    13561 gccaccacct tttgtttgcc ggttaaaacg acgattgcca ccacgctgtt tatgatgttt
    13621 ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc aattaccaaa
    13681 aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac catagggcta
    13741 ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc aaccgtcggt
    13801 tatgcctgtg cggcacttat gttctccatt ggcggcttta tttttatgat gctgtgctac
    13861 agaggcgtca aagagcatta tgtggacaca gcgccaaccg gacataaagc cagtattctc
    13921 aaatcttttt gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat tgctaacctg
    13981 tgtactctgg cggcatttaa tatcaaactg gcgattcagg tctattacac ccagtatgtg
    14041 ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg catcctcgtc
    14101 ggcgtattac tggtgccagt aactgtaaaa tgttttggta aaaaacaggt ttatttagct
    14161 ggcatggtgc tatgggcggt gggtgatata ctgaattatt tctggggaag taactctttc
    14221 actttcgtca tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa cagcctgaac
    14281 tgggcactgg taccagatac cgtagattac ggtgaatgga aaaccggtat tcgtgccgaa
    14341 ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact tgctggcttc
    14401 ttgccaggca ttatgctgac gcaaattggt tatgttccca acatcgcgca aagcgatgcg
    14461 acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc aattattgct
    14521 gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcatt gattattgag
    14581 gaaatcaacc aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc ttccgtaacg
    14641 ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc tacgtttaaa
    14701 aaatggactt attatgaaaa agattaatgc aataatttta ttatcttctc tgacctctgc
    14761 ctcggtattt gctggcgcat acgtcgaaaa cagggaggca tataatcttg cctccgatca
    14821 gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta ttatgttaac
    14881 caatacctac acctttcagc gagaagatga actaaaacat ggctataatg aaattgaagg
    14941 ttggtatcct ttattcaaac caaccgataa attaaccatc cagcccggtg gcttaattaa
    15001 tgataagagt atcagttcgg gtggtgcagt gtatctggac
//
LOCUS       DAJTUN010000099        14980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTUN010000099 DAJTUN010000000
VERSION     DAJTUN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.099"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1109)
                     /locus_tag="OO470_002923"
     CDS             complement(<1..1109)
                     /locus_tag="OO470_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6252113.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSH"
     gene            complement(1308..1392)
                     /locus_tag="OO470_002924"
     tRNA            complement(1308..1392)
                     /locus_tag="OO470_002924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1356..1358),aa:Leu,seq:caa)
     gene            1588..2607
                     /gene="ahr"
                     /locus_tag="OO470_002925"
     CDS             1588..2607
                     /gene="ahr"
                     /locus_tag="OO470_002925"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6252114.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2736..4238
                     /locus_tag="OO470_002926"
     CDS             2736..4238
                     /locus_tag="OO470_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6252115.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4357..5439)
                     /gene="lptG"
                     /locus_tag="OO470_002927"
     CDS             complement(4357..5439)
                     /gene="lptG"
                     /locus_tag="OO470_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6252116.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5439..6539)
                     /gene="lptF"
                     /locus_tag="OO470_002928"
     CDS             complement(5439..6539)
                     /gene="lptF"
                     /locus_tag="OO470_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6252117.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6806..8317
                     /gene="pepA"
                     /locus_tag="OO470_002929"
     CDS             6806..8317
                     /gene="pepA"
                     /locus_tag="OO470_002929"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6252118.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8451..8894
                     /gene="holC"
                     /locus_tag="OO470_002930"
     CDS             8451..8894
                     /gene="holC"
                     /locus_tag="OO470_002930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6252119.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8894..11749
                     /gene="valS"
                     /locus_tag="OO470_002931"
     CDS             8894..11749
                     /gene="valS"
                     /locus_tag="OO470_002931"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6252120.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11803..12999)
                     /locus_tag="OO470_002932"
     CDS             complement(11803..12999)
                     /locus_tag="OO470_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6252121.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13192..13695
                     /locus_tag="OO470_002933"
     CDS             13192..13695
                     /locus_tag="OO470_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6252122.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13741..14157)
                     /gene="rraB"
                     /locus_tag="OO470_002934"
     CDS             complement(13741..14157)
                     /gene="rraB"
                     /locus_tag="OO470_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6252123.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14319..>14980
                     /gene="argF"
                     /locus_tag="OO470_002935"
     CDS             14319..>14980
                     /gene="argF"
                     /locus_tag="OO470_002935"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6252124.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VA"
BASE COUNT         3854 a         3837 c         3855 g         3434 t
ORIGIN      
        1 tggtgactca tctgccgctc taccgcatcc ctcgaccata atcccgactc aatcaacgaa
       61 ctacaagcca tcgtcctgaa gccatgccca caaacctcga ccttcgtgtc ataccccatt
      121 acgcgcaaag ccttgttcac cgtattctca ctcatcggct tacgtggatc gtgatcgccc
      181 acaaaaatca gctctcgatt tccgctcatg cttttgatct tttccaaaat acttagggcc
      241 tgccgcgaca aaggtacaag gtgtggtgtc cgcatcttcg agccacgctg agaatgcttg
      301 actccttcca gttgctcacg ctcacctggg attgtccaca tagccgtttc aaaatctatt
      361 tctgaccagc gggcaaaacg cagctcgctc gaacggatga aaaccaataa agtgagttca
      421 accgccagtc gagttaacgg tctgccagaa tagtgatcta tgcgatgaag taattcaggt
      481 atacggttca gttcaagagc tgcacggtgc tgtcttttcg ccgtagcaac cgcacctgca
      541 atctcttgcg cggggttgta gtcgattaag ccgctctgaa cggcaaagcg cataatcgcg
      601 gtagtacgct gctgtaaacg ggcggcaact tcgagccgcc cggatgactc gactgcttta
      661 atgggtacaa gaagatcccg cgtcttcagt tccgcaatgt tccgcttacc gatagcagta
      721 aagagattat cctccagact tttcaaaaca cgagcgctat gcgatgccga ccacttctga
      781 ttgctggcgt gccagtctct ggcgactact tcaaacgtta tcgcctcttg ttcctgttct
      841 accttaacag ctttcttgtt ttcactggga tcgacaccat tagctaaaag cttacgagcc
      901 tcatcccggc gtgccctggc atccgccaac gacacttcgg ggtatttccc caatgccagc
      961 atcttctctt tgccgccaaa gcgataacgt agccgccagt atttggagcc gttagggtga
     1021 accagcaaaa ccatgccttc accgtcggtc agcttatagg cttttgcttc aggcttagcc
     1081 gaacgaacct tcacatcact cagagccatg atgagtatcc tttcaagggt tctgtgtggg
     1141 tacaaacatt atcgaaccgg gaaatacccg cagttgtacc cgcatcagta agttgatgta
     1201 gattgaatca ggttgactta ggttgagtga aaaagcgagg aaagccttgc ggatactgga
     1261 ttttaggcac aaaaaaagac gtccgttgac gtctattgat gttccgatgg tgccgaaggc
     1321 cggactcgaa ccggcacgta tttctacggt tgattttgaa tcaactgcgt ctaccgattt
     1381 cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc
     1441 accatgcaag cgccagcttg cacccacctc gctaagtgct gaaattttca gcattacact
     1501 cagcgatatg taacctttgt cacactccag gcaccccgcc ctgccatgct ctacacttcc
     1561 caaacaacac cagagaaggc cccaaaaatg tcgatgataa aaagctatgc cgcaaaagaa
     1621 gcgggcggcg aactggaagt ttatgagtac gatcccggtg agctgaagcc acaagatgtt
     1681 gaagtgcagg tggattactg cgggatttgt cattccgatc tgtcgatgat cgataacgaa
     1741 tggggatttt cacaatatcc gctggttgcc gggcatgagg tgattgggcg cgtagtggca
     1801 ctcgggagcg ccgcgcagga taaaggtttg caggtcggtc agcgtgtcgg gattggctgg
     1861 acggcgcgta gctgtggtca ctgcgacgcc tgtattagcg gtaatcagat caactgcgag
     1921 caaggtgcgg tgccgacaat tatgaatcgc ggtggctttg ccgagaagtt gcgtgcggac
     1981 tggcaatggg tgattccact gccagaaaat attgatatcg agtccgccgg gccgctgttg
     2041 tgcggcggta tcacggtctt taaaccactg ttgatgcacc atatcactgc taccagccgc
     2101 gttggggtaa ttggtattgg cgggctgggg catatcgcta taaaacttct gcacgcaatg
     2161 ggatgcgagg tgacggcctt tagttctaat ccggcgaaag agcaggaagt actggcgatg
     2221 ggtgccgata aagtggtgaa tagccgcgat ccgcaggcac tgaaagccct gtcggggcag
     2281 tttgatctca ttatcaatac tgtgaacgtc agcctcgact ggcagcctta ttttgaggcg
     2341 ctgacctatg gcggtaattt ccatacggtc ggtgcggttc tcacgccgct gtctgttccg
     2401 gcctttacgt taattgcggg cgaccgcagc atctctggtt ctgctaccgg cacgccttat
     2461 gagctgcgaa agctgatgcg ctttgccgcc cgcagcaagg ttgcgccgac aaccgaactg
     2521 ttcccgatgt cgaaaattaa cgacgccatt cagcatgtac gcgatggtaa agcgcgctac
     2581 cgagtagtcc tgaaagccga cttctgatcc ctttgccggg tgctgtcgcc cggcacttaa
     2641 atctctttgg aaggcgtctc gcaatctgct gaatttagcg gtgaagagtg cacagccgtt
     2701 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     2761 gcacgccgga cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta
     2821 ctggcgcaac ggggacgggt aaaaccgtta cgctgcaaaa actggcagag tcattgtcgg
     2881 aaatcggcgt gccggtgttt atggctgatg tgaaaggcga tctgaccggt atcgcgcagg
     2941 caggaacggc gtcggaaaaa ctgctcacaa ggcttaaaaa tatcggcgtc aatgactggc
     3001 aaccgcatgc caatccggtg gtggtgtggg atatctttgg cgagaaaggc cacacggtgc
     3061 gggcgacggt ttcggatctg gggccgctgt tgctggcgcg actgttgaat ctcaacgatg
     3121 tgcaatctgg cgtgctgaat attatcttcc gcattgcgga cgatcaggga ttgttgctgc
     3181 tcgactttaa agatttgcgg gcgattaccc agtacatcgg cgataacgcc aaatccttcc
     3241 agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc ggattactgt
     3301 cgctggaaca gcaaggcgca gcacacttct ttggtgagcc gatgctggat atcaaagact
     3361 ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt
     3421 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttgtatgaac
     3481 aattgccgga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaggcac
     3541 atctgctgtt taacgatgca ccgcaggtac tgctggataa gattgagcag gtgataaggc
     3601 ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtct gatattccgg
     3661 ataatgtgtt cgtccagctc ggtaatcgtg ttcagcacgc tttgcgggct tttacgccaa
     3721 aagatcagaa agcagtgaag acagcggcgc aaaccatgcg ggccaatccg gcatttgata
     3781 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcaa
     3841 aaggaagtcc ttctgtggtg gaacgggcga tggtgatcgc accttgttcg cgaatggggc
     3901 cggtgacgga agatgagcgt aatggcctga ttaatcactc cccggtgtat ggcaaatatg
     3961 aagatgaggt ggaccgcgag tccgcctatg agatgctgca aaaaggcttt caggccagta
     4021 ccgagcagca aaataatccc cccgtgaaag ggaaagaggt ggcggtggat gacggtattc
     4081 ttggtggatt gaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg
     4141 gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg
     4201 ggatgttggg gagtttgctg ggggggagaa aaagataagt cagacgtgat ctgtgcccct
     4261 caccctaacc ctctccccaa aggggcgagg ggaccgttcg aactcgtatt tgactttgtc
     4321 aacaataata aagggagctt tcgctccctt gttagattac gattttctca ttaacagcca
     4381 caggctgatt aagaagaagc tggcacttgg caacagtgcg ccgatgatcg gcgggatgcc
     4441 gtaaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct
     4501 gatacctgtg accacacgca cgcccatcgg tacgctacgc agtgggccaa agataaacga
     4561 cagcgccatc agcatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt
     4621 gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacgt agttgtgcaa
     4681 accactgatg gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag
     4741 gtcggttttc caggtgccgc tcaccgtctg cgaaccggta atctgtttcg gattggtcag
     4801 atcagattca tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcggt
     4861 agcggcataa cgtacggatt gcagacgacg attctcgtta aaggcataaa tactgatgcc
     4921 acctaacacc tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc
     4981 ccataaacct tgctgggtag agagcaacga gccgccgtac atcgcctgcg cacggtagtt
     5041 acgcgccatc tgttcgccct gcggcgcgac ccattcgcca atcaccatcg tcagcaagac
     5101 cagtggaatg gcggttttca tcaccgacag cgctacctgc atacgggtaa aaccagaagc
     5161 ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag
     5221 aagagccgcc atcgggaaga agatctgcac atctttcggc acgctcagca aggtatacat
     5281 tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaattt
     5341 gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt
     5401 tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa
     5461 aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa
     5521 gccagataaa tcaggttaac ggtccacatc cacagtgtcg ggtccagctt acctttaccg
     5581 ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag catggctggc
     5641 agcatcgaca gtacgcgtcc ctgacgcggg ttaaccacgc tcaacggtac aaccataagt
     5701 gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc gcgagcacga
     5761 tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc
     5821 gccaccgcct ggtgaccaat gatcgcctga taatcctgga aatccgtaat gcggaaatca
     5881 cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg
     5941 tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct
     6001 tttggtcgaa tttgtgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg
     6061 aacagcaccg agctgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg
     6121 ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccacggtccc
     6181 gcccacatca cgttaaccgc cgcgacgatt gccgtgaata ccgcaaggat cattgccgct
     6241 ttcaccagaa ccgctttgct caagccgcag gcatgcatta ccgtaatttc actttcggta
     6301 tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc
     6361 gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg
     6421 tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg
     6481 aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact
     6541 ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac
     6601 gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca
     6661 cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc
     6721 atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg
     6781 tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa
     6841 acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc
     6901 agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgcc ggggcgaact
     6961 ggaaggaaaa ccggggcaga cattgttgct gcaccatgtt ccgaatgtac tttccgagcg
     7021 aattctcctt attggttgcg gcaaagaacg tgagctggat gagcgtcagt acaagcaggt
     7081 tattcagaaa accattaata cgctgaatga tactggctcg atggaagcgg tctgctttct
     7141 gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac
     7201 agcaaaagag acgctctaca gtttcgatca gttgaaaacg aataagagcg aaccgcgtcg
     7261 tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg
     7321 cgcgatccag cacggtctgg cgattgccgc cgggataaaa gcagcgaaag atctcggtaa
     7381 tatgccgccg aatatctgta acgccgctta tctcgcctct caggcgcgcc agctggctga
     7441 cagctacagc aagaatgtca tcacccgcgt tatcggcgaa cagcagatga aagagctggg
     7501 aatgcactct tatctggcgg tcggtcaggg ttcacaaaac gaatcgctga tgtcggtgat
     7561 tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt
     7621 aaccttcgac tccggcggta tctccatcaa gccttcagaa ggcatggatg agatgaagta
     7681 cgatatgtgc ggcgcggcgg cagtttacgg cgtgatgcgt atggtcgcgg agctgcaact
     7741 gccgattaac gttattggcg tgctggcagg ctgtgaaaac atgcctggcg ggcgtgccta
     7801 tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga
     7861 tgccgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgaacgtt ttgagccgga
     7921 agcagtgatt gacgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcatat
     7981 tactggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg cgtctgaaca
     8041 atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac agctggagtc
     8101 caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg
     8161 cttcctgtca cgctttaccc gtaagtataa ctgggcgcac ctggatattg caggaaccgc
     8221 ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt
     8281 cctgttaaac cgcgctgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta
     8341 accctctccc taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt
     8401 cagggtgagg aatgcagcaa tatttaaatc caccacaaga agccccatat atgaaaaacg
     8461 caacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac
     8521 tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtactc atcgcctgtg
     8581 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
     8641 ttgttccgca taatttagcg ggagaaggac cgcgcggcag tgcgccggtg gagatcgcct
     8701 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
     8761 cagattttgc caccgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
     8821 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
     8881 caacctggaa ataatggaaa agacatacaa cccacaagat atcgaacagc cgctttacga
     8941 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
     9001 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
     9061 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctgtggca
     9121 ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc
     9181 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
     9241 atggaaagcg gaatctggcg gcaccattac ccgtcagatg cgccgtctcg gcaactccgt
     9301 cgactgggag cgtgaacgct tcaccatgga cgaaggcctg tccaatgcgg tgaaagaagt
     9361 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aagcgcctgg taaactggga
     9421 cccgaaactg cgtaccgcta tttctgacct ggaagtggaa aaccgcgaat cgaaaggttc
     9481 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta
     9541 tctggtggtt gcgaccactc gcccggaaac cctgctgggc gataccggtg tagctgttaa
     9601 cccggaagat ccgcgttaca aagatctgat tggcaaatat gtcattctgc cgctggttaa
     9661 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
     9721 gaagatcacc ccggcgcacg actttaacga ctatgaagtc ggtaaacgtc acgccctgcc
     9781 gatgatcaac atcctgacct ttgacggtga tatctgtgaa agcgcccagg tgttcgatac
     9841 caaaggtaac gaatctgacg tttattccag cgagatcccg gcagagttcc agaaactcga
     9901 gcgttttgct gcacgtaaag cagtcgttgc tgcggttgat gcactcggcc tgctggaaga
     9961 aattaaaccg cacgacctga ccgttcctta cggcgaccgt ggcggcgtgg ttatcgaacc
    10021 gatgctgacc gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg cagttgaagc
    10081 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    10141 gatgcgcgat attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc
    10201 ggcatggtat gacgaagcgg gtaatgttta tgttggccgc aacgaagaag aagtgcgcaa
    10261 ggaaaataac ctcggtgcca acgttgccct gcgtcaggac gaagacgttc tcgacacctg
    10321 gttctcttct gcactgtgga ccttctctac ccttggctgg ccggaaaata ccgacgccct
    10381 gcgtcagttc cacccaacca gcgtgatggt atctggtttc gacatcatct tcttctggat
    10441 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    10501 gccgttccac actgtttaca tgaccggtct gattcgtgac gacgaaggtc agaagatgtc
    10561 caaatccaag ggtaacgtta tcgacccgct ggatatggtt gacggtattt cgctgccaga
    10621 actgctggaa aaacgcaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa
    10681 gcgcaccgag aaacagttcc cgaacggcat tgagccgcac ggcaccgatg ccctgcgctt
    10741 caccctggcg gcgctggcgt ctaccggtcg tgacatcaac tgggatatga agcgtctgga
    10801 aggctaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    10861 agaaggtcag gattgcggct tcaacggcgg cgaaatgacg ctatcgctgg cggaccgctg
    10921 gattctggcg gagttcaacc agactatcaa agcgtaccgc gaagcgctgg acagcttccg
    10981 cttcgatatc gccgcaggca ttttgtatga gttcacctgg aaccagttct gcgactggta
    11041 tctggagctg accaagccgg taatgaacgg tggaacagaa gccgaactgc gcggtactcg
    11101 ccatacgctg gtgactgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    11161 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    11221 catgctgcag ccgttcccgc agtatgatgc atctcaggtt gatgaagccg cactggccga
    11281 caccgagtgg ctgaaacagg cgatcgttgc tgtacgtaac atccgtgcag aaatgaacat
    11341 cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg
    11401 cgtaaatgaa aaccgtggct tcctgcaaac gctggcgcgt ctggaaagca tcaccgtgct
    11461 gcctgccgat gacaaaggtc cggtttccgt taccaagatc atcgacggcg cagagctgct
    11521 gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgcctgg cgaaagaagt
    11581 ggcgaagatc gaaggtgaaa tcagccgcat cgagaacaag ctggcaaatg aaggctttgt
    11641 cgcccgcgca ccggaagcgg ttatcgcgaa agagcgtgag aagctggaag gttatgcaga
    11701 agcgaaagcc aagctgattg aacagcaggc tgttatcgcc gcactgtaat caacacagtg
    11761 atgaaaacga aggccggagc atgctccggc cttttttatc tcttacagaa atggtaaaga
    11821 aggcataact ccccgagaaa tactggctaa gaagccttta tctggttgtt cttctttatt
    11881 gattaaagga aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat
    11941 catccatatt ttcagcagtg ggtaagccag accacccgta atcccggata tcaccaccaa
    12001 cgcacacatg cgataaagca taccgtggta cgttaaagtt gagcgaaaac gaatacgccc
    12061 atcattcagt gacaggttgc tcataaaatg atttcgcaac gacaccgtta aataactggt
    12121 gctaacagca atcccaaaat aataaattaa ctgcgcgatt atcattttac gttgcatttc
    12181 cataaattgc tgtaaattct caatatcatc atttgcatac ccagaactat catactcatt
    12241 taatatttga tcgaagataa tataaccagc aacagcgaga aaaagtaata aagctaaaaa
    12301 tgcgaggatg gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat
    12361 accgaaactg gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa
    12421 aataccaatg gaaacaatac caactattgc catcagaacc acagatataa taacactact
    12481 tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc
    12541 cattaaaatc ggcaagaaaa aggttaccca ccagaaccct ttcaaagagc aattaaaact
    12601 aaatcttacg ccgttgagac tggtcattaa ggcctgataa cgcagtcctt ttgctgccat
    12661 aaatataagc aaaaccaaca gtaacaaagt caaaacacag ccaacaagcg gcatatctgc
    12721 tgacactgtc attaagattg cgaaatagaa aaaaacaaaa acaagacaac taacaaaaac
    12781 attcccaccg gtaattccat aagaaaatcg ttgtccgtta acttccatat tagcataaag
    12841 ataacgctta catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa
    12901 caacacattc accatacaaa taagaaaata tctctcacct gtaccatgaa aaacaaaaga
    12961 atgggacgga acatccattt cattaataac ttgagccata aaaatcccct gaattaaaaa
    13021 atccattgat ataatttata gatacagaca gtaaaatgtt ctgccacgtt acaatactct
    13081 tatcagtaat aggctggcaa aaactttatt gctttataag acgcttgcgc acgtaagaag
    13141 agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac
    13201 attgcgccgc aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc tgctatcgcc
    13261 agcgtcatcc gtcaggttta cgcagagtat ggtcttaccg ccgataaagg ctacaccgtt
    13321 gccgatccga atctcgacga actgtatcaa gtgtacagcc agcctggcca tgcttattgg
    13381 gtggttgaat acaatggcga agtggtcggc ggcggcggga ttgcgccatt aacaggtagt
    13441 gaagcggaca tttgcgaact gcagaaaatg tactttcttc ccgctattcg cggtaaaggg
    13501 ctagcgaaaa aactggcctt aatggcgatg gagcaggcac gagagatggg cttcaaacgc
    13561 tgctatctgg aaacgaccgc ttttttaaag gaagccattg cgctttatga gcatttgggc
    13621 tttgagcata tcgactatgc gcttggctgc acgggccatg tcgattgtga agtgcggatg
    13681 ctacgcgaac tctaaaaaaa tgcccgctag tatgtgctat cgggcatttt aatcaattaa
    13741 ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc catcttcgcc
    13801 gttaggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag
    13861 cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag
    13921 gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac
    13981 ttcgtaaccg agtttaaacg cttcaactgc cgctttttcc agagtttcca gatcgtctgc
    14041 ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt ccagtaattc
    14101 ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc
    14161 tcattcctct taatgtcctg ccgatactct tattgtcaca caccactgac attgcctcca
    14221 cctttgtgac aaagatttat gctttagact tgcaaatgaa taataataca cataaattga
    14281 attttaattc attgaggcgt tagccacagg agggatctat gtccgggttt tatcataagc
    14341 atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg
    14401 cgaagctgaa agctgataag aaaagcggta aagaagaagc caaactcact ggtaaaaaca
    14461 tcgcgctcat cttcgaaaaa gactcgactc gtacccgatg ctctttcgaa gttgccgcat
    14521 atgaccaggg ggctcgcgtt acttatcttg gcccaagcgg tagtcagatt ggtcataaag
    14581 agtcgattaa agacactgcc cgcgtgcttg gtcgcatgta tgacggtatt cagtatcgcg
    14641 gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg
    14701 gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc
    14761 atttacccgg taaagcattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca
    14821 acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg
    14881 cgccacaggc atgctggcca gaagctgcgc tggttacgga atgccgcgcc ctggcacaac
    14941 aaaatggcgg gaatattacg ctgacggaag atgtagctaa
//
LOCUS       DAJTUN010000100        14824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTUN010000100 DAJTUN010000000
VERSION     DAJTUN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.100"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            40..678
                     /locus_tag="OO470_002936"
     CDS             40..678
                     /locus_tag="OO470_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6252125.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            681..1844
                     /locus_tag="OO470_002937"
     CDS             681..1844
                     /locus_tag="OO470_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6252126.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1928..3553
                     /locus_tag="OO470_002938"
     CDS             1928..3553
                     /locus_tag="OO470_002938"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6252127.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3670..3945)
                     /gene="yjfN"
                     /locus_tag="OO470_002939"
     CDS             complement(3670..3945)
                     /gene="yjfN"
                     /locus_tag="OO470_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6252128.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4094..4423)
                     /gene="bsmA"
                     /locus_tag="OO470_002940"
     CDS             complement(4094..4423)
                     /gene="bsmA"
                     /locus_tag="OO470_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6252129.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4605..5354
                     /gene="yjfP"
                     /locus_tag="OO470_002941"
     CDS             4605..5354
                     /gene="yjfP"
                     /locus_tag="OO470_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6252130.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5351..6106)
                     /gene="ulaR"
                     /locus_tag="OO470_002942"
     CDS             complement(5351..6106)
                     /gene="ulaR"
                     /locus_tag="OO470_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6252131.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6214..7278)
                     /gene="ulaG"
                     /locus_tag="OO470_002943"
     CDS             complement(6214..7278)
                     /gene="ulaG"
                     /locus_tag="OO470_002943"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6252132.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7633..9030
                     /gene="ulaA"
                     /locus_tag="OO470_002944"
     CDS             7633..9030
                     /gene="ulaA"
                     /locus_tag="OO470_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6252133.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9046..9351
                     /gene="ulaB"
                     /locus_tag="OO470_002945"
     CDS             9046..9351
                     /gene="ulaB"
                     /locus_tag="OO470_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6252134.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9361..9825
                     /gene="ulaC"
                     /locus_tag="OO470_002946"
     CDS             9361..9825
                     /gene="ulaC"
                     /locus_tag="OO470_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6252135.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9839..10489
                     /gene="ulaD"
                     /locus_tag="OO470_002947"
     CDS             9839..10489
                     /gene="ulaD"
                     /locus_tag="OO470_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6252136.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10499..11335
                     /gene="ulaE"
                     /locus_tag="OO470_002948"
     CDS             10499..11335
                     /gene="ulaE"
                     /locus_tag="OO470_002948"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6252137.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11335..12021
                     /gene="ulaF"
                     /locus_tag="OO470_002949"
     CDS             11335..12021
                     /gene="ulaF"
                     /locus_tag="OO470_002949"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6252138.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12150..12425)
                     /locus_tag="OO470_002950"
     CDS             complement(12150..12425)
                     /locus_tag="OO470_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6252139.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12752..13147
                     /gene="rpsF"
                     /locus_tag="OO470_002951"
     CDS             12752..13147
                     /gene="rpsF"
                     /locus_tag="OO470_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6252140.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13154..13468
                     /gene="priB"
                     /locus_tag="OO470_002952"
     CDS             13154..13468
                     /gene="priB"
                     /locus_tag="OO470_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6252141.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13473..13700
                     /gene="rpsR"
                     /locus_tag="OO470_002953"
     CDS             13473..13700
                     /gene="rpsR"
                     /locus_tag="OO470_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6252142.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13742..14191
                     /gene="rplI"
                     /locus_tag="OO470_002954"
     CDS             13742..14191
                     /gene="rplI"
                     /locus_tag="OO470_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6252143.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14262..>14824)
                     /locus_tag="OO470_002955"
     CDS             complement(14262..>14824)
                     /locus_tag="OO470_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6252144.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3464 a         3622 c         3996 g         3742 t
ORIGIN      
        1 atttttaacg cagcttgtat gacatggtaa tggacgatca tggccagaaa acgcaaatca
       61 agaaataaca gtaaaattgg tcacggagcg atcagtcgca ttggtagacc gaataatcct
      121 tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa cgttggcgct aatgggcggt
      181 gccgcttttt tcgtattgaa aggttgtagc gatagtagcg atgtcgataa cgacggcgac
      241 ggaacatttt acgcgacagt gcaggattgt attgatgacg gtaataatgc cgacatttgc
      301 gcgcgtggct ggaacaacgc caaagcggca ttttatgccg atgttccaaa gaatatgact
      361 cagcagaact gtcagtctaa gtacgaaaat tgctactacg acaatgttga acagagttgg
      421 atcccggtcg tttctggatt tttgttaagc cgggttattc gtaaagatcg cgatgagccg
      481 ttcgtctata acagcggcgg atcctcattt gcttcccgcc ccgtctggcg caacacttcc
      541 ggtgattact cctggcgctt cggttctggc aaaaaagagt cttactcttc aggcggcttt
      601 accaccagaa aagcgtcgac cgtttcgcgc ggcggctatg gtcgttcttc cagcgcccgt
      661 gggcattggg gaggctaatc atgctgagac acaacgttcc tgtgcggcgg gatctggacc
      721 agatcgccgc taacaacggt ttcgactttc atatcatcga caatgaaatc tattgggacg
      781 agagtcgggc ttaccgtttt actctgcgcc agattgaaga gcagatcgaa aaaccgactg
      841 cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc ggttaaagac gaagagatcc
      901 tgacgcaact ggcgatcccg ccgttgtact gggatgtgat cgccgaaagc tggcgcgcac
      961 gcgatccttc gctgtatggc cggatggatt ttgcctggtg tggcaatgcg ccggtgaagc
     1021 tgctggagta caacgccgat acgccgacct cgttgtatga gtcggcttat ttccagtggt
     1081 tgtggctgga ggatgcccgg cgcagcggca ttattccgcg tgatgccgat cagtacaacg
     1141 ccattcagga acgcctgatt tcgcgcttta gtgagcttta cagtcgggaa ccgttttatt
     1201 tttgctgctg tcaggacacc gatgaagaca ggagtaccgt gctgtacttg caggactgcg
     1261 cccagcaggc agggcaggag tcgcggttta tctacattga agatctcggt ttgggcgtcg
     1321 gcggcgtact gaccgatctt gatgataatg tcatccagcg tgcatttaag ctgtatccgc
     1381 tggagtggat gatgcgtgac gataacggtc cgctgctgcg caagcgtcgc gagcaatggg
     1441 tggagccgtt atggaaaagt atcttgagta ataaagggct aatgccgctg ctttggcgct
     1501 tcttccctgg tcatcctaac cttcttgcat cctggtttga gggtgaaaaa tcgcagattg
     1561 ccgctggcga aagctatgtg cgtaaaccga tctactcgcg cgaaggcgga aacgtcacca
     1621 ttttcgacgg tcagaataac gtcgttgacc acgctgatgg tgattacgcc gatgaaccga
     1681 tgatctacca ggcgtttcaa cctctgccgc gatttggcga tagctacaca ctcatcggta
     1741 gctggattgt cgatgatgaa gcgtgcggaa tggggatccg tgaagataac acactgatca
     1801 ccaaagacac ctcacgtttc gttccgcatt acattgctgg ataagaatgt tttagcaatc
     1861 tctttctgtc atgaatccat ggcagtgacc atactaatgg tgactgccat tgatggaggg
     1921 agacacagtg cactggcaaa ctcacaccat ttttaatcaa cctataccat taaataacag
     1981 caacttatac ctgtctgatg gcgcgctttg cgaagcggta acgcgtgaag gtgctggctg
     2041 ggatagcgat tttctagcca gtattggtca gcagttagga acggctgaat cccttgaact
     2101 ggggcggctg gcgaatgtaa acccgcctga actgttgcgc tacgatgccc aggggcggcg
     2161 tctggacgat gttcgtttcc atcccgcatg gcacctgctg atgcaggcgt tatgtaccaa
     2221 tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc ggcgcatttg tggcgcgcgc
     2281 ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg ttatgtccaa taacgatgac
     2341 ctttgccgcc acgccattgt tgttacagat gttacccacg ccgtttcagg actggaccac
     2401 gccgctgctg agcgatcgct acgattctca cttattgcca ggtgggcaaa aacgcggttt
     2461 gttgattggc atgggaatga cggaaaagca gggcggttcc gatgtcatga gcaacaccac
     2521 ccgtgcagag cgtttggaag atggctctta tcggctggtg gggcataaat ggtttttctc
     2581 ggtgccgcaa agcgatgcgc atctggtgct ggcgcagacc gcgggcggtc tgtcctgctt
     2641 ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg attcgcctcg agcggctgaa
     2701 agataagctg ggtaatcgct ctaacgccag ttgcgaagtg gagtttcagg atgccattgg
     2761 ctggttattg gggcaggaag gggaaggaat tcgtctgatc ctgaaaatgg gggggatgac
     2821 gcgttttgat tgcgccctgg gtagccatgc catgatgcgc cgtgcatttt cgctggcgat
     2881 ttatcatgca catcaacgcc atgtttttgg taatccattg atccaacagc cccttatgcg
     2941 tcatgtctta agtcgcatgg cacttcagct tgaagggcaa acggcgttgc tgtttcgtct
     3001 tgcgcgagcg tgggaccggc gtgccgatgc caaagaagcc ctgtgggcgc gtttatttac
     3061 gcctgcggca aaatttgtga tctgcaaacg aggtatgccg tttgtggccg aagcgatgga
     3121 ggtgctgggc ggcattggtt attgcgagga gagcgagctg ccgcggcttt accgggagat
     3181 gccggtaaac agtatttggg aaggttccgg caatattatg tgcctggatg tgttgcgcgt
     3241 tctcaataag caagcgggcg tatacgactt attgtcggaa gcatttgtgg aagtgaaagg
     3301 gcaggatcgc tattttgatc gcgcggttcg tcgtttacag cagcagctgc gtaagccagc
     3361 tgaagaactg gggcgagaga ttactcatca gctattcctg ctgggctgcg gtgcgcaaat
     3421 gttgaaatat gcttctccgc caatggcgca ggcgtggtgt caggtgatgt tagatacgcg
     3481 cggcggcgta cggttgtcag agcagatcca gaatgattta ttgctgcggg cgacgggggg
     3541 agtgtgtgtg taagcgtata cgactgatgc gacgctggtt tcgattaact aaatgaaata
     3601 tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc cggcattgtt catttgctgt
     3661 agatgacact catgcataca gtatcgcctg tacgcgccag ttatcaggct gctggtcttc
     3721 gtacatggta ataatgcggt accatgacgc accttgttca tcggccttta acgccacgat
     3781 acgttccacg tcctgcggat cccctgaaat attgctaact gagatctgac ctatttcatt
     3841 caggcccgtc acgcagtcag cactggcgaa ttctgcagac tgtgccgtgg cgctcgcagg
     3901 ataagtcact aattgcagcg agggtgaggc aagtaattgt ttcatcgtca gctccatttt
     3961 tcgtttcccc acatgttgca gggcattcca tcgcggtaat aaacgcccaa gcttccagcc
     4021 ggtaatttat tgtgcacagg taaacgcata gggacgcaaa gcaatgtaaa gctgactatg
     4081 taagttgtct gatttattta cggtacaaaa tggcttgtga ataccactgt cctgtcacga
     4141 tggtttcgtc caccatgacg acaacgtaat aatcagcttt tgcagcgaca gcttttgctc
     4201 tgatttctgc taatgcgtca tccggagaac cccgaaccat cgtgcttacg ctacctattc
     4261 gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg atggtccgtt actggcggtg
     4321 ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa catcaacacc aataataaac
     4381 tggcaaaccg gtaaataacg ctattacgtt tcctgctaac catagtgtag tgccttatta
     4441 atttaacttt gggggaatgt tcccgaattg ttacctggta cgcgattttc gaatgaaaac
     4501 gtgacgaaag cgtaacccac atctcaacat tatgaactaa taccgccagc agaaacttgt
     4561 gctaaggatt tcttcatgtc ataccagaca aaaaggagag acagatgatt gaaatagaat
     4621 cacgcgagct ggcagatatt cccgttcttc atgcttatcc tgtcgggcaa aaagataccc
     4681 cgttaccgtg cgtaattttt tatcacggct ttacttcatc cagcctggtg tatagctatt
     4741 ttgccgttgc gctggcgcag gctggtttgc gggtgatcat gccggatgcg cccgatcacg
     4801 gtagccgttt tagtggcgac gaagcgcggc ggttaaatca attctggcaa atcttgctac
     4861 aaagtatgca ggaattcact actttacgtg cggcaatagc cgaagaaaac tggctgcttg
     4921 atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat gacggcactg ggaattaccg
     4981 ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg atcgggctat tttacatcgc
     5041 tcgcccgttc actgtttcca ccgctgatac ctgaaacggc agcacagcag aatgaattca
     5101 ataaaattgt cgcgccgctg gcagagtggg aagcgacaaa ccacctggaa caacttgggg
     5161 acagacctct actgctgtgg catggcctcg acgatgacgt tgtgcctgcc gacgaatcac
     5221 tacgtttgca gcaggcctta agcgagacgg gacgggataa actgctaacc tgttcatggc
     5281 agccaggcgt gcgtcaccgt attacgcctg aggcgttaga tgctgccgtg acatttttcc
     5341 gccagcatct ttaaacacgc agaatgctga ccccttgcgc ttccagttgt tgcaggattt
     5401 ccgggttagc atttttgccg gtgatcagca tatcgatttg atcggcacgg ctaaaaagca
     5461 ttcccgcgcg ttcgccaatc ttactgctat caaccagtac caccagtttc cctaccacgc
     5521 tcagcatctt ctgctctgcc attgctgtca gcatatcggt tttatacaac ccttctgcgg
     5581 tcagcccttt tccgctggta aacatccagt gcccggcata gagactgttt tcgctgccct
     5641 gcgggcttaa agtgatggac tgacttttgt tgtactgtcc acccataatg atcacgctgt
     5701 catgttcttg atcgatcagg taatttgcca gcggtagata attagtgatg atttgcactg
     5761 gcttgccaca catttcccgc ccaagcagaa acgcggtgga gccgcagttg atgactacgc
     5821 tttcgcccgg attaaccagc tgcgaggccg ctttagcaat acgtactttt tcatcgtgat
     5881 tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg ctgttgggta atagcttctg
     5941 cgccattgcg cacctttttc agtttgccgc tttcgccaag tttattgata tcgcgtcgcg
     6001 cagtggcagg cgaaattccc agacgctcaa cgactttctc aacggtcaca aagcccaatt
     6061 gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc agtcatgagc tattccgata
     6121 aaatttgatt tttttagatg atatttgaaa tagccaggaa atactacgcc ggatagcgcg
     6181 aacttctcca cgctatccgg tataaacgac agattacagg aatgacttga acggcagatc
     6241 cggttcaata gtgaagcaat catcgaaacc gcgcggatag tggtactcga agttgtcttt
     6301 atccagcggc caggtaaatt tgccgccaac ctgccagata aacggcttga agccatactt
     6361 cagacgatct tttttgatct cccacagcac gcggatctct tgcggatcgg cctggaagtt
     6421 tgaccagatg tcgtggtgga acgggataac cactttcgca ttcagggctt cacccatacg
     6481 cagcatatcg gcgctggtca ttttgtcggt aataccgcgc gggttctcac catacgatcc
     6541 taacgccaca tcgatctgat gttcgttacc gtgcttcgca taatagttag agtagtggga
     6601 atcgccactg tgatataggg aaccgccagg ggttttgaac aggtagttta ctgcgcgatc
     6661 gtccatgcca tctggcagta cgccagccgc tttttgatcg gcaggcaggg tgatcagtgc
     6721 agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct ttcactttta caacatcgcc
     6781 cggtttgacc acgatgcaac gctctttcgg tacgccccag ccaatccaca aatcaacgca
     6841 ggttttcggt ccaatgaatg gtacgtcatc agcacagttc tgcatcacgg cagcagcgac
     6901 gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt accgcgtcga tctggcgaat
     6961 cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt ggctgcagtt ttttcacgcc
     7021 agccatgcgc tgcatctggt gaccctgttt cattaacggg ttaccgtgac tttgtttgcc
     7081 agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct tctgatttca accagatccc
     7141 ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg acctgctctt gttcaatttc
     7201 ttcattcagc cagctacccc actccgggaa agtgctcagg atccaggatt ctcgggtgat
     7261 acttttcact ttactcatcg ccatttacct tcatgatagt tcaattcgaa tcaacatgtg
     7321 attggttttg attaatcctg acactattcc ttcaggaagg caatgaccat tttttgactt
     7381 tttgccaggg aagttgttgt tgattttgag tgtggaaaga tttaatggaa tgtgtagtta
     7441 aattaactaa atgaatttaa ataaataatt gtttcgttgt gtgaatcaca ctctatccgt
     7501 gtggaattat ttgcgggtcg cgccacattt aatcataaat aatcttgttg tgattacttt
     7561 tgaaaattag agtgagtaca caacattccg ggtgtgcgga atacccggtt acctcttctt
     7621 caggagatcg ttatggagat cctctacaac atctttaccg tgttttttaa ccaggtcatg
     7681 accaatgccc cgttgttgct gggtattgtg acctgtctgg gctacatcct actgcgcaaa
     7741 agtgtcagcg ttattattaa aggcacgatt aaaaccataa ttggtttcat gttgttgcag
     7801 gcagggtccg gcatcctcac cagcaccttc aaaccggtgg tggcgaaaat gtccgaagtc
     7861 tacggcatta acggcgcaat ttccgatact tacgcgtcga tgatggcaac catcgaccgc
     7921 atgggcgatg cctatagctg ggtaggttac gcggtgttgt tagcgctggc gctgaacatc
     7981 tgttacgtgc tgctgcgtcg cattaccggt attcgtacca tcatgctgac tggtcatatc
     8041 atgttccagc aggcggggct gattgccgtt acgttgttta ttttcggcta ctccatgtgg
     8101 acaaccatta tctgcacggc gattctggtt tcgctctact ggggcattac ctccaacatg
     8161 atgtacaagc cgactcagga agtgacggac ggctgcggtt tctccatcgg tcaccagcaa
     8221 cagtttgcat cattaattgc ctataaagtt gcgccgttcc tcggcaaaaa agaggaaagc
     8281 gttgaagacc tcaaactgcc aggctggctg aatattttcc acgacaacat cgtctccacg
     8341 gcgattgtca tgaccatctt ctttggtgcc attctgctct ccttcggtat cgacaccgtg
     8401 caggcgatgg caggcaaagt gcactggacg gtctatatcc tgcaaaccgg tttttccttc
     8461 gcggtggcga tcttcatcat cactcagggt gtgcgcatgt ttgtggcgga actctctgaa
     8521 gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg ttctggcgat tgactgtgcg
     8581 gctatttata gcttcgctcc gaacgccgtg gtctggggct ttatgtgggg caccatcggt
     8641 cagctgattg cggttggcat cctggtcgcc tgcggttcct cgatcctgat tatcccaggc
     8701 tttatcccga tgttcttctc caacgccacc atcggcgtgt tcgctaacca cttcggcggc
     8761 tggcgtgcgg cgctgaagat ttgtctggtg atggggatga tcgaaatctt tggttgcgtc
     8821 tgggtggtga aacttaccgg tatgagtgcc tggatgggga tggcggactg gtcgattctg
     8881 gcaccgccga tgatgcaggg cttcttctcc atcggtatcg cctttatggc cgtcatcatt
     8941 gtaattgcac tggcttatat gttcttcgct ggccgcgcac tgcgcgcaga agaagatgca
     9001 gaaaaacaat tggcagaaca gtctgcttaa taaggagttt tgattatgac cgtacgtatt
     9061 ctggctgtgt gtggcaacgg acaaggcagt tccatgatca tgaagatgaa agtggaccag
     9121 tttttaaccc aatcaaacat tgaccacacg gtaaatagct gcgcggttgg cgaatacaaa
     9181 agcgagttga gcggcgcgga tatcatcatc gcttctacgc acattgcggg cgaaatcacc
     9241 gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc tctctcctgc cgactttggc
     9301 ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg atgtgaagta aggacgcgcc
     9361 atgaaattac atgattcgct ggcggagaat aaatccatcc gcctgcaggc tgaagctgag
     9421 acatggcagg acgcagtaaa aatcggcgtt gacctgctgg tggcggcgga tgttgtcgag
     9481 ccgcgttact accaggcgat tctggatgcg gttgaacagc acggtccgta ctttgtgctc
     9541 gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg gcgttaagaa aaccggtttt
     9601 gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg aagataacga cccggtggat
     9661 atcctcatca ccatggcggc ggtcgatgcc aacacgcacc aggaagtggg catcatgcag
     9721 atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt tacgtgcctg ccgtaccgag
     9781 caggaagtac tggatctcat tgaccgcacc aacgcggcag cttaagaagg aattgaagat
     9841 gtcattaccg atgttgcaag tcgcgctgga caaccagact atggatagcg cctacgaaac
     9901 cactcgcctg attgccgaag aagtcgacat tatcgaagtg ggtaccattc tgtgcgtggg
     9961 cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac ccgcacaaaa tcgtgctggc
    10021 agacgccaaa attgccgatg cgggcaaaat tctttctcgt atgtgcttcg aagccaacgc
    10081 tgactgggtg accgtgattt gttgtgcgga tatcaacacc gccaaaggtg cgctggacgt
    10141 agcaaaagag tttaacggcg acgtgcagat cgaactgacc ggttactgga cctgggaaca
    10201 ggcgcaacag tggcgcgacg caggtattca gcaggtggtt tatcaccgca gccgtgacgc
    10261 gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact gcgatcaaac gtctttccga
    10321 tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg gaagatctgc cgctgttcaa
    10381 aggcattcca attcatgtgt ttatcgccgg tcgcagcatc cgtgatgccg cttctccggt
    10441 tgaagccgcg cgtcagttca aacgttccat cgccgaactg tggggctaag gagcggatat
    10501 gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg ctccccgccg gggagtgctg
    10561 gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat tttgtcgaga tgtcggtaga
    10621 tgaaactaac gagcgcctgt cgcgcctcga ctggagccgc gaacagcgtc tggcgctggt
    10681 gaatgctatt gttgaaaccg gcgtgcgtgt gccgtccatg tgcctttctg cccatcgtcg
    10741 tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag gggctggaga ttatgcgtaa
    10801 agccatccag ttcgcccagg acgttggcat tcgcgtgatc cagctggcgg gctatgacgt
    10861 ttactatcag gaagccaata acgaaacgcg tcgtcgtttc cgtgacggcc tgaaagagag
    10921 cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg gagatcatgg attatccgtt
    10981 gatgaactcc atcagcaagg cgctgggtta cgcgcactat ctcaacaacc cgtggttcca
    11041 gctctacccg gatatcggca acctgtcggc gtgggacaac gatgtgcaga tggagttgca
    11101 ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac accaaaccgg gcgtcttcaa
    11161 aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt tgcttcgaaa cgctcaaaca
    11221 gagtggctat tgcgggccgt acctgattga gatgtggagc gaaacggcgg aagacccggc
    11281 ggcagaagtg gtgaaagcgc gcatggccaa agcaggcatg gtggaggcgg cataatgcaa
    11341 aagctaaaac agcaggtgtt tgaagccaac atggatctgc cgcgctatgt gctggtgacc
    11401 tttacttggg gcaacgtcag cgctatcgac cgcgaacgcg ggctggtggt gatcaagccc
    11461 agcggcgttg cctacgaaac catgaaagca gacgatatgg tggtggtcga tatgagcggc
    11521 aaggtggtgg aaggggagta tcgcccatct tccgacactg cgacgcatct cgaactctac
    11581 cgtcgttacc cgtcgcttgg cggcattgtc catacccact ccactcatgc caccgcatgg
    11641 gcgcaggcgg ggctggcgat cccggcatta ggcaccacgc acgctgacta cttctttggt
    11701 gatatcccgt gtacgcgtgg attaagcgaa gaagaggtgc agggtgagta tgaactgaac
    11761 actggcaaag tgattatcga aacgctgggc aacgccgagc cgctgcatac gccggaaatt
    11821 gtggtgtatc agcacggacc gttcgcctgg gggaaagatg cccacgacgc ggtgcataac
    11881 gcggtagtga tggaagaagt ggcgaaaatg gcgtggattg cgcgcagtat taatccacaa
    11941 ctcaatcata tcgacagcta cctgatgaat aaacacttca tgcgtaagca cggtcctaac
    12001 gcttattacg ggcagaagta aaacacgcgc tgcggaaatt tccttcctcg ggagataact
    12061 ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc gaagggggca aagcaaacta
    12121 tggcaatgtt tcgttggtta tacctggtgc tagcgataaa tatccgcgct ggcgtgcata
    12181 ttgccgttac tccccggttc ccgcatcaga attacgtggt agtacgttgc gccttgcgca
    12241 tctgtttctt catttaatgc ctgacgtgct tcgctttcag tggcgaaatt atgattgata
    12301 taaatcacgc ctaagctttg cacatcgtcc atatttctgg cctggtggtt attaatttca
    12361 atggctgccc atgtatttgc acttagcaaa agcacagcca gaagggctaa aactcgactg
    12421 aacatagata cctcctcgac ggctgacttt gtgtgctctc ctctgtgatg atcttctgat
    12481 ttaattttaa tcaatgataa agaagttgat ggtgaccatt tctgatgcag ttgttcaaaa
    12541 aaacactgtg acggaatgtg atgaacttca aatcagcggt ttagaggtta tttgcgaaag
    12601 gggagattta tttctgctcc gcccttgagt ttagcggggt atgccagtac tataacgcgt
    12661 catttttcag ccgaccttta acacgttcct tgcctccccg ggattcggct gacccagaca
    12721 ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat tacgaaatcg tttttatggt
    12781 ccatcctgac cagagcgaac aggttccggg catgatcgag cgctacactg ctgccatcac
    12841 tggtgcagaa ggcaagatcc accgtctgga agactggggc cgccgtcagc tggcttaccc
    12901 gatcaacaaa ctgcacaaag cacactacgt tctgatgaac gttgaagctc cgcaggaagt
    12961 gatcgatgag ctggaaacta ccttccgctt caacgatgcc gttatccgca gcatggttat
    13021 gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt aaagcgaaag acgagcgccg
    13081 tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat gctgaagctg gggattctga
    13141 agagtaattt ctgatgacca accgtctggt gttgtccggc accgtgtgca ggactcccct
    13201 tcgaaaggtc agtccatcag gaattcctca ctgccagttc gtgcttgagc atcgttctgt
    13261 gcaggaggaa gccggctttc accggcaggc atggtgtcaa atgcccgtta ttgttagcgg
    13321 acacgaaaac caggccatta ctcacagtat aacggtcggc agtcgcataa ccgttcaggg
    13381 gttcatttca tgccacaagg caaagaacgg actgagcaaa atggttttgc atgccgagca
    13441 gattgaattg atagattctg gagactagcc atatggcacg ttatttccgt cgtcgcaagt
    13501 tctgccgttt caccgcggaa ggcgttcaag agatcgacta taaagatatc gctacgctga
    13561 aaaactacat caccgaaagc ggtaagattg tcccaagccg tatcaccggt acccgtgcaa
    13621 aataccagcg tcagctggct cgcgctatca aacgcgctcg ctacctgtcc ctgctgccgt
    13681 acactgatcg ccatcagtaa tcggtcacgg tccattaata cgactttgag aggataaggt
    13741 aatgcaagtt attctgcttg ataaagtagc aaacctgggt agcctgggtg atcaggtaaa
    13801 cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag ggtaaagctg ttccagctac
    13861 caagaaaaac attgaattct tcgaagcacg tcgcgctgaa ctggaagcta aactggctga
    13921 agttctggca gctgctaatg ctcgcgctga gaaaatcaat gcactggaaa ctgttaccat
    13981 cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc atcggtactc gcgacatcgc
    14041 tgacgctgta actgcagctg gcgttgaagt ggctaagagc gaagttcgtc tgccgaacgg
    14101 cgttctgcgt accactggcg aacacgaagt gagcttccag gttcacagcg aagtattcgc
    14161 gaaagtgatc gtaaacgtag tagctgaata attcgttatt caacgagacg taaaaacgcc
    14221 gaccattggt cggcgttttg ctttctattt ttcgtcaggc attaatttcg caagtagatc
    14281 cattcaccat tctcttgtgt ccataagtca ggatggtttg ctggcgttag atgcatacga
    14341 tcactcctta tgtctcggtc ctgcggcact cgctcgaaac ctaaacccga gtagattgcg
    14401 gctgaatttg ataaggaacg tagaccaatt ttcaaatcat ggggtaaatc agaatacgcg
    14461 tttagcaatg cagcaatgat gaatccgagg gcattagaaa cgcctgtaac agtgtgaagt
    14521 tggtcgatat aaagatcgat tgttggtagt tcagatgacc agcaattagg catttgggca
    14581 ggaagggtta caacgtatcc tatcgtttgg ccggtatagt ctggagtact ataaccaggt
    14641 cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt cgaaggattc gacatcttgc
    14701 aataaatatt tcagttctgc ggttaacctc gcacccgcgt aagctttatt gtatttctgg
    14761 gcgtgaatat tatctaatgt agcttcatgg ttccatccaa aagaattatc accattcatt
    14821 gtta
//
LOCUS       DAJTUN010000101        14317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTUN010000101 DAJTUN010000000
VERSION     DAJTUN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.101"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO470_002956"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="OO470_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6252145.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="OO470_002957"
     CDS             complement(1150..1236)
                     /locus_tag="OO470_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6252146.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO470_002958"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OO470_002958"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6252147.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OO470_002959"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OO470_002959"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6252148.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="OO470_002960"
     CDS             3585..3629
                     /locus_tag="OO470_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252149.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OO470_002961"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OO470_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6252150.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO470_002962"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO470_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6252151.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO470_002963"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO470_002963"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6252152.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO470_002964"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO470_002964"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6252153.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO470_002965"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO470_002965"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6252154.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO470_002966"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO470_002966"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6252155.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO470_002967"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO470_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6252156.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO470_002968"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO470_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6252157.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO470_002969"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO470_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6252158.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..>14317
                     /gene="thiB"
                     /locus_tag="OO470_002970"
     CDS             13616..>14317
                     /gene="thiB"
                     /locus_tag="OO470_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6252159.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAGA"
BASE COUNT         3744 a         3795 c         3508 g         3270 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cggcgcg
//
LOCUS       DAJTUN010000102        14280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTUN010000102 DAJTUN010000000
VERSION     DAJTUN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.102"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(30..902)
                     /gene="rluF"
                     /locus_tag="OO470_002971"
     CDS             complement(30..902)
                     /gene="rluF"
                     /locus_tag="OO470_002971"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6252160.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1250..2197
                     /locus_tag="OO470_002972"
     CDS             1250..2197
                     /locus_tag="OO470_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6252161.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2267..3073
                     /locus_tag="OO470_002973"
     CDS             2267..3073
                     /locus_tag="OO470_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6252162.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3083..3490
                     /locus_tag="OO470_002974"
                     /pseudo
     CDS             3083..3490
                     /locus_tag="OO470_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3490..3984
                     /locus_tag="OO470_002975"
     CDS             3490..3984
                     /locus_tag="OO470_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6252163.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4050..4847
                     /locus_tag="OO470_002976"
     CDS             4050..4847
                     /locus_tag="OO470_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6252164.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            4858..5646
                     /locus_tag="OO470_002977"
     CDS             4858..5646
                     /locus_tag="OO470_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6252165.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5698..6927
                     /gene="sorE"
                     /locus_tag="OO470_002978"
     CDS             5698..6927
                     /gene="sorE"
                     /locus_tag="OO470_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6252166.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7309..7998
                     /gene="pepE"
                     /locus_tag="OO470_002979"
     CDS             7309..7998
                     /gene="pepE"
                     /locus_tag="OO470_002979"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6252167.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVLLEAGHRF"
     gene            complement(8089..9720)
                     /locus_tag="OO470_002980"
     CDS             complement(8089..9720)
                     /locus_tag="OO470_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6252168.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9940..13623)
                     /gene="metH"
                     /locus_tag="OO470_002981"
     CDS             complement(9940..13623)
                     /gene="metH"
                     /locus_tag="OO470_002981"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6252169.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13823..>14280
                     /locus_tag="OO470_002982"
     CDS             13823..>14280
                     /locus_tag="OO470_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6252170.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGG"
BASE COUNT         3364 a         3781 c         3741 g         3394 t
ORIGIN      
        1 tggacgtttt catcagtctg aggcgttaat cagcgtccct tctttttacg ccccggcgag
       61 gtaaaacgct taccgttgga cgccgggcga ccgccttttt ccgccgtttt ttccatctta
      121 acgactggac gtttgatgcc cgctgttttc ggtttggcct tcgctttagg ttttgcctcg
      181 gaagaggaat tttcgatgag cttaaagagg tcgattaact catcgtcggt taaatagcgc
      241 cactccccca gcggaatgcc gcttaagctg acgttcataa tgcgcgtacg ttccagcttt
      301 ttcacttcat agccgaaatg ctcgcacatg cgccggatct gacggttcag cccctgcact
      361 agggtaatgc ggaagacaaa cggcgcttct tttttgactt tgcacttttt cgtcaccgtc
      421 ccgaggatcg gcacccccgc ccccatacca cgaataaatt catcggtaat cggtttatcg
      481 accgtcacca gatactcttt ctcatgatcg ttacccgcac ggaggatctt attcaccaga
      541 tcgccgtgat tggtgaggaa aatcagcccc tgggagtctt tatccaggcg gccaatcggg
      601 aacacgcgtt tgctgtggtt aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg
      661 gtgctgacaa taccgacggg cttgttcagg gcgataagta ccaaatcttc ggcttcccga
      721 ggctcaatca actgaccgtt tactttcaca atgtcgccgg gtttcacctg atcgccaatg
      781 gtggctcgct tgccattaag gaacacattg ccttgctcga tatagcgatc cgcttcgcgg
      841 cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac ggactgatga gtcgggcagc
      901 ataaattctc ctgtaaaacg cggaatatac cgtacctctt agcggggaaa aacagggtta
      961 tgcggcaacg tcgaaattaa atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg
     1021 aagtacgatg caaatttgcc tggcaaaata aggataatca acatattacg aatcgctgca
     1081 cacctgtgct gatgactact acgcagagaa acaaatgtgc aagagaaagc gctatttctc
     1141 cccatgcaaa gagtgaaaaa tcgctttgaa aggccctcat gatggggtta tatcagatca
     1201 agcggtgaga aagcgtattt gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag
     1261 tgacgatatc cgtttgattg tgaagattgc ccaactctat tacgaacagg atatgacgca
     1321 ggcgcaaatc gcgcgcgaac tgggtattta ccgcaccacc atcagccgct tgcttaaacg
     1381 aggccgcgat cagggaatgg tcaccatcgc catcaactat gactacaacg aaaatctctg
     1441 gctggagcag caactgaagc aaaagtttgg cctgaaagac gttgtggtgg tgtcgggaaa
     1501 tgatgaggat gaagagactc aactggcgat gatggggtta cacggcgcgc aactgctgga
     1561 tcgcttgctg gagcctggcg atattgtcgg tttttcctgg ggtcgcgcgg tgagcgcact
     1621 ggttgaaaac ttgccgcagg cggggcaatc gcggcagtta atctgcgtac caattattgg
     1681 cggaccgtcc ggtaaactcg aaagccgcta tcatgtaaac acattaacct acagcgcggc
     1741 agcgaagctg aaaggggaat cgcatctcgc ggattttccg gctctgctgg ataacccatt
     1801 aattcgtaat gggatcatgc agtctcagca ctttaaaacc atctctgcct actgggataa
     1861 tctggatatc gccctggtgg gaattggctc accggccatt cgcgacggcg ctaactggca
     1921 tgcgttttat ggtggtgaag agagtgacga cctgaatgcc cgccaggttg ctggcgatat
     1981 ttgctcgcgc ttttttgata ttcacggcgc aatggttgaa acgaatatga gcgaaaaaac
     2041 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
     2101 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
     2161 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
     2221 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
     2281 aaatttgcag gataaaatca ttattgtcac cggcggcgca tccggtattg gtctggcgat
     2341 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
     2401 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
     2461 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
     2521 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
     2581 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
     2641 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
     2701 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
     2761 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
     2821 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
     2881 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
     2941 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
     3001 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     3061 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     3121 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttgc
     3181 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggagaagt tagtaagcat
     3241 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtagaatgc
     3301 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     3361 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     3421 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     3481 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     3541 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     3601 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     3661 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     3721 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     3781 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     3841 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     3901 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     3961 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcat
     4021 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     4081 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     4141 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     4201 tactcggcgg tacgctggaa ttgatagctc tctgctggat gaacgtcggc gcggcgcaat
     4261 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     4321 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     4381 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     4441 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     4501 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     4561 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     4621 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     4681 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     4741 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     4801 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     4861 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     4921 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     4981 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gctacaccta
     5041 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     5101 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     5161 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     5221 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     5281 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     5341 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     5401 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     5461 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     5521 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     5581 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     5641 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     5701 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     5761 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     5821 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtgacgacc
     5881 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     5941 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     6001 cgcccggatt gccccggcta ctccttcccg tgggtagacg gcgaagcgac gcatgtggtt
     6061 attcccaacg aggtcatgga acaatattgc ctgctggcat acgacggcga aacctatttt
     6121 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     6181 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     6241 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     6301 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     6361 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     6421 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     6481 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     6541 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     6601 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     6661 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     6721 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     6781 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     6841 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     6901 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     6961 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     7021 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     7081 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     7141 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     7201 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     7261 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     7321 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     7381 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     7441 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     7501 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     7561 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     7621 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     7681 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     7741 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     7801 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     7861 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     7921 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tctgctggaa
     7981 gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
     8041 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     8101 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     8161 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     8221 tacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     8281 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     8341 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     8401 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     8461 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     8521 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     8581 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     8641 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     8701 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     8761 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     8821 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     8881 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     8941 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     9001 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     9061 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     9121 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     9181 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     9241 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     9301 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     9361 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     9421 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     9481 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     9541 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     9601 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     9661 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     9721 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     9781 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     9841 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattaag
     9901 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     9961 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
    10021 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
    10081 ataccaaccc gaaaccgatg caccaggcca catcgcgaaa gattctgtga gtttcatgcc
    10141 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
    10201 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
    10261 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
    10321 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
    10381 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
    10441 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
    10501 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
    10561 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
    10621 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
    10681 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
    10741 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
    10801 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
    10861 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
    10921 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
    10981 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
    11041 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
    11101 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggcgaagc cctgacgctc
    11161 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
    11221 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
    11281 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
    11341 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
    11401 ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
    11461 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
    11521 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
    11581 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
    11641 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
    11701 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
    11761 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
    11821 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
    11881 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
    11941 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
    12001 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    12061 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    12121 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    12181 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    12241 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    12301 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    12361 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    12421 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    12481 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    12541 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    12601 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    12661 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    12721 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    12781 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    12841 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    12901 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    12961 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    13021 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    13081 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    13141 cgccgccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    13201 agacgccgtg cggttggttg ggccaagaac accggcaacg tagcgcggtt tctccggcgt
    13261 gcgcgcggtc cactcgtcag cacaggcgcg cgccagtttc gccgccgcaa agttgatttc
    13321 cgccgacagg gattccatct ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt
    13381 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    13441 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    13501 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    13561 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    13621 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactgga
    13681 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    13741 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccac
    13801 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    13861 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    13921 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgt cgcaacaagc
    13981 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    14041 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    14101 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    14161 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    14221 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
//
LOCUS       DAJTUN010000103        14250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTUN010000103 DAJTUN010000000
VERSION     DAJTUN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.103"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..114)
                     /locus_tag="OO470_002983"
     CDS             complement(<1..114)
                     /locus_tag="OO470_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6252171.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASM"
     gene            388..3912
                     /gene="nifJ"
                     /locus_tag="OO470_002984"
     CDS             388..3912
                     /gene="nifJ"
                     /locus_tag="OO470_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6252172.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4279..5408
                     /gene="ompN"
                     /locus_tag="OO470_002985"
                     /pseudo
     CDS             4279..5408
                     /gene="ompN"
                     /locus_tag="OO470_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5549..5983
                     /gene="uspF"
                     /locus_tag="OO470_002986"
     CDS             5549..5983
                     /gene="uspF"
                     /locus_tag="OO470_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6252173.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6159..7094
                     /gene="ttcA"
                     /locus_tag="OO470_002987"
     CDS             6159..7094
                     /gene="ttcA"
                     /locus_tag="OO470_002987"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6252174.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7223..8590)
                     /gene="dbpA"
                     /locus_tag="OO470_002988"
     CDS             complement(7223..8590)
                     /gene="dbpA"
                     /locus_tag="OO470_002988"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6252175.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9068..10051)
                     /gene="zntB"
                     /locus_tag="OO470_002989"
     CDS             complement(9068..10051)
                     /gene="zntB"
                     /locus_tag="OO470_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6252176.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10306..11538
                     /gene="dgcM"
                     /locus_tag="OO470_002990"
                     /pseudo
     CDS             10306..11538
                     /gene="dgcM"
                     /locus_tag="OO470_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11559..12122)
                     /gene="smrA"
                     /locus_tag="OO470_002991"
     CDS             complement(11559..12122)
                     /gene="smrA"
                     /locus_tag="OO470_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6252177.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12376..13830)
                     /locus_tag="OO470_002992"
     CDS             complement(12376..13830)
                     /locus_tag="OO470_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6252178.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(13827..>14250)
                     /locus_tag="OO470_002993"
     CDS             complement(13827..>14250)
                     /locus_tag="OO470_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6252179.1"
                     /translation="FSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVSLVPVSE
                     LWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATG
                     NFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3797 a         3592 c         3366 g         3495 t
ORIGIN      
        1 catcgacgct tttaaacttg gcgctacgtg cgccgcagtc gcctgctgaa caggctcact
       61 actacacgcc gacaacaata aagcggcaca ccctacccaa aacgctgctc gcatctcttc
      121 ttcctctgat cttcaagcca aacgacaccg ccataaataa taggcagcac agagggcggc
      181 gtcgagagct gtcctgcgcg ttgccccgca tttttacttt tttatggcta tttttttgcc
      241 ctctgtttga tcaaaacatt cattactctg atgtggggga cacaaaagcg aaaatgcaga
      301 agaaagccat ttgctaaaat tgaaggatta ctactgggcg cgcagcaatt tcgtgcgccc
      361 ctcatttgcg caatgtaagg gtgtcatatg attactattg acggtaatgg cgcggttgct
      421 tcggtgacgt ttcgcatcag tgaagttatc gccatctacc ctattacccc cagttccacg
      481 atggcagaac aggctgatgc ctgggccgga aacggcttga agaacgtttg gggagacaca
      541 ccacgcgtgg ttgaaatgca gtcggaagcg ggtgctatcg ctaccgtgca tggcgctttg
      601 cagacgggtg ccctttcaac atcgtttacg tcatcgcagg gtttgctgct gatgatcccg
      661 acgctgtaca aactggcagg cgaactgacg ccatttgtcc tgcatgtagc ggcacgtacc
      721 gttgccacac atgcactttc tatttttggc gatcattccg acgttatggc ggtgcgccag
      781 acgggttgcg cgatgttgtg tgcggctaac gtccaggaag cgcaagactt tgctctcatt
      841 tcgcacatcg caacgctgaa gagccgcgtg ccatttattc atttctttga tggttttcgc
      901 acgtcccacg aaatcaataa aattgtcccg ctggccgatg acactattct tgatctcatg
      961 ccgcaggccg aaattgatgc tcatcgcgcc cgggcgctca acccggaaca tccggtgatc
     1021 cgcggtacgt ccgccaatcc tgacacttat ttccagtctc gcgaagccac caatccctgg
     1081 tacaacgcgg tctatgacca tgttgaacag gcgatgaatg atttcgctgc cgcgacaggt
     1141 cgtcagtatc agccgtttga gtattacggg catccgcaag cggaacgggt gattatcctg
     1201 atgggctctg ccattggcac ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa
     1261 aaagttggcg tgctgaaagt tcgtctgtac cgtcccttct ccgccaaaca cttgctgcaa
     1321 gctctgccgg gatccgtacg cagcgtggcg gtactggaca gaaccaaaga acccggtgcc
     1381 caggcagaac cgctctatct ggatgtgatg accgcactgg cagaagcctt taataatggt
     1441 gagcgcgaaa ccctgccccg tgtcattggt gggcgctatg gtctttcatc caaagaattt
     1501 ggcccggact gtgtactggc ggtatttgcc gagctcaacg cggctaaacc taaagcacgc
     1561 tttacggttg gtatttacga tgatgtgacc aatctgtcac tgccgttgcc ggaaaacact
     1621 ctgccaaact cggcgaaact tgaagccttg ttttatggcc ttggtagtga tggcagcgtt
     1681 tccgcaacca aaaacaatat caagattatc ggtaattcca cgccgtggta cgcacagggc
     1741 tattttgttt acgactccaa aaaagcgggc ggcctgacgg tttctcacct tcgcgtgagc
     1801 gaacagccga ttcgttccgc ttatctcatt tcccaggctg attttgttgg ctgccaccag
     1861 ttgcagttta tcgataaata tcagatggct gagcgtttaa aacctagcgg cattttcctg
     1921 cttaatacgc cgtacagcgc agatgaagtg tggtcgcgct tgccgcagga agttcaggcc
     1981 gtgttaaacc agaaaaaagc gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa
     2041 tgtggcctgg cggcccgtat taataccgtc atgcagatgg cttttttcca tctgacgcaa
     2101 attctgcctg gcgatagcgc cctcgcagaa ttgcagggtg cgattgccaa aagttacagt
     2161 agcaaaggtc aggatctggt ggagcgcaac tggcaggctc tggcgctggc gcgtgaatcc
     2221 gtagaagaag ttccgttgca accggtaaat ccgcacagcg ccaatcgacc gccagtggtt
     2281 tccgatgccg cccctgattt cgtgaaaacc gtaaccgcgg cgatgctcgc cgggcttggt
     2341 gatgccctcc ccgtttcggc gctgccgcca gacggcacct ggccgatggg caccactcgc
     2401 tgggaaaaac gcaatatcgc cgaagagatc cccatctgga aagaggaact ctgtacccaa
     2461 tgtaaccact gcgtcgccgc ttgcccacac tcagctattc gcgcaaaagt ggtaccgcct
     2521 gaagcgatgg aaaacgcccc tgccagcctg cattcgctag atgtgaaatc gcgtgatatg
     2581 cgcgggcaga aatatgtctt gcaggtggca ccggaagatt gcaccggttg taacctgtgc
     2641 gtcgaagttt gcccggcgaa agaccgtcag aatccagaga ttaaagccat caatatgatg
     2701 tctcgccagg aacatgtcga agaagagaaa atcaattacg atttcttcct caacctgcca
     2761 gaaatcgacc gtagcaaact ggaacgtatt gatattcgta catcgcagct gattacaccg
     2821 ctgtttgaat attcaggtgc ttgccccggt tgtggcgaga cgccgtatat taaattgctg
     2881 actcagctct atggtgaccg gatgttgatc gctaacgcca ccggctgttc ttcaatttat
     2941 ggcggtaacc tgccctctac accgtatacc accgatgcca acggtcgtgg gccggcatgg
     3001 gcgaactctc tatttgaaga taatgccgaa tttggccttg gtttccgcct gacggtcgat
     3061 caacaccgtg tccgcgtgct gcgtctgctg gatcaatttg ccgataaaat cccggcggaa
     3121 ttactgacgg cgttgaaatc agacgctacg ccagaggttc gtcgagaaca ggtcgcagct
     3181 ttacgccagc aactcaacga tgttgccgaa gcacatgaac tgctgcgtga cgcagatgca
     3241 ctggtggaaa aatcaatctg gctgattggt ggcgacggct gggcttacga tatcggcttt
     3301 ggcggtctgg atcatgtatt gagtttgacg gaaaacgtca acattctggt gctggatacg
     3361 caatgctatt ccaacaccgg tggtcaggcg tcgaaagcga caccgctggg tgcagtaact
     3421 aagttcggcg agcacggcaa acgtaaagcg cgtaaagatc ttggcgtcag tatgatgatg
     3481 tacggtcatg tttatgtggc gcagatttct ctcggcgcgc agctgaacca gacggtgaaa
     3541 gcgattcagg aagcggaagc gtatccggga ccatcgctga tcattgctta tagcccgtgt
     3601 gaagagcatg gttacgatct ggctctcagc cacgaccaga tgcgccaact cactgctacc
     3661 ggcttctggc cgctatatcg ctttgatccg cgtcgtgccg atgaaggcaa actgccgctg
     3721 gcgctggatt cacgcccgcc gtcagaagca ctggaagaaa cgttacttca cgagcagcgt
     3781 ttccgtcgtc tgaattcgca gcagccagaa gtggcagaac agttatggaa agatgctgca
     3841 gctaatttgc aaaaacgcta tgacttccta gcacaaatgg ccggaaaagc ggaaaaaagc
     3901 aacaccgatt aaatgctctg gataaggatt atccaattct aaaaaaaaag cccggacgac
     3961 tgttcgggct tgtcttttta tatgttggaa aatcagtggc aatgcaatgg cccaacagaa
     4021 aataaaatct gtgacaataa aggcatataa cccgcgcaga ataacgtata ggaaattaaa
     4081 agtataattt ttattttgta tgtattatct tcgatacatt caccagactt attatcattt
     4141 cagaggaatt atttgattaa ggttttactt aaggcgtaac aaatgatatt tatcggctaa
     4201 ctgaacttct cctttaggat gttttcactc cccgaaatgg ggataaaagg caaataaaat
     4261 aactaaagga tttattcaat gaaaagaaaa gtactggcac ttttaattcc tgccctgctc
     4321 gccgcaggtg ctgcacatgc agccgaagtt tataataaag acggcaacaa attagatctg
     4381 tatggcaaag ttgatggcct gcattatttt tctgataatt cagcgaaaga tggcgaccag
     4441 agctatgcgc gtctgggttt taaaggcgaa acccaaatta acgatcaact tactggctac
     4501 ggtcaatggg aatacaatat tcaggcaaac aacactgaat cttcaaaaaa ccagtcatgg
     4561 acccgtctgg catttgccgg actgaaattt gcagattacg gttctttcga ttacggacgt
     4621 aattatggcg taatgtacga catcgaaggc tggaccgata tgctgcctga atttggcggt
     4681 gactcttata ccaatgcaga caactttatg actggtcgag ccaatggcgt cgcgacttat
     4741 cgtaatactg atttcttcgg tctggtaaat ggtctgaact tcgcggtgca gtatcaaggt
     4801 aacaacgaag gagccagtaa tggtcaggaa ggcaccaata acgaacgcga tgttcgtcat
     4861 gaaaacggtg acggctgggg tctttccaca acatatgatt taggcatggg ctttagcgct
     4921 ggtgcggcat acacctcttc tgaccgcacc aatgaccagg ttaaccatac tgcggcgggt
     4981 ggtgataaag cagacgcgtg gactgctggg ctaaaatacg atgctaacaa tatttaccta
     5041 gcaaccatgt attcagaaac gcgtaatatg accccgtttg gcgacagcga ttatgcagtt
     5101 gcgaacaaaa cccagaactt tgaagttact agtaccagtt tgattttggt ctgcgtccgg
     5161 cagtctcttt cctgatgtct aaaggccgtg accttcacgc tgctggtggt gcagacaacc
     5221 cggcaggtgt tgatgataaa gatctggtta aatacgccga tgttggcgcg acttactatt
     5281 tcaataaaaa catgtccacc tatgttgact ataaaatcaa cctgttggat gaagatgaca
     5341 gtttctacgc tgccaacggc atctctaccg atgatattgt cgctttaggt ctggtttatc
     5401 agttctaaat cctcctgccc gctgttatgg cgggcttttt ctgcttattc ttcctccttt
     5461 gatctaaatt aaaaatgtga actccgtcat tacacaaaaa gtgtcatctg gcgttacact
     5521 ttatgcggat actaaaacag gaggttttat gaacagaacg attcttgtcc ctatcgatat
     5581 ttcagattca gaattaactc aacgcgtgat tagccacgtt gaggcagagg caaagattga
     5641 tgatgcagag gttcatttcc tgacggtaat accttcactg ccctactatg cctctctggg
     5701 tttagcgtat tccgcagaat taccggcaat ggatgacctg aaagcggaag ccaaatcgca
     5761 actggaagag atcattaaaa aatttaaact gccaaccgac agagtgcatg tccatgttga
     5821 ggaaggctcg cccaaagacc gcattctgga attggcgaag aagatccccg ctcatatgat
     5881 catcattgct tcccatcgac cggatatcac cacttatctg ctcggttcca acgccgcagc
     5941 tgtagtgcgt cacgcagagt gctccgtgct ggttgtgcgc tgacactaac gcccgcacat
     6001 tgctgcgggc tttttgattc atttcgcaaa tgtgctgaca ttttcccctc taatccgtac
     6061 catacacgcc acagttttta tatcagattt cttatgctga gcgttccggc atgacgcata
     6121 ctcttctgat gccatataac gaattgagtc gcttttaaat gtcgcaaaat caagaaatta
     6181 gtaagaaaga acaatacaac ctgaacaaat tacaaaaacg tctgcgtcgt aacgtgggcg
     6241 aagccattgc tgacttcaat atgattgaag aaggcgatcg catcatggtt tgcctctccg
     6301 ggggtaaaga cagctatacc atgctggaga ttctgcgcaa tttgcagcaa agcgcgccaa
     6361 tcaatttttc gctggtggct gttaacctcg atcaaaagca accgggcttc ccggaacacg
     6421 ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa gattgttgaa gagaacactt
     6481 acggtatcgt gaaagagaag attccagagg gcaaaaccac ttgctcactg tgttctcggc
     6541 tacgtcgcgg tatcctttat cgcaccgcaa cggaactggg gacgacgaag atcgcgttgg
     6601 gtcaccatcg tgacgatatc ctgcaaacgt tgttcttaaa tatgttctac ggcggcaaga
     6661 tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa acatatcgtt attcgtccac
     6721 tggcctactg ccgcgagaaa gatattcagc gatttgccga tgcaaaagcg ttcccgatta
     6781 ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg tcaggtgatt gctgacatgt
     6841 tgcgtgactg ggataaacgt tatccagggc gtatcgagac gatgttcagc gcgatgcaga
     6901 atgtggtgcc gtcgcatctg tgcgatacca acctgttcga tttcaaaggc attacccacg
     6961 gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg cgaagagatc ccactacaac
     7021 cggcgggctg gcagccagaa gaagatgaaa atcagttgga tgagttacgg ctgaatgtgg
     7081 ttgaagtgaa ataaccaaga tagcgcccga tgcgcaagcg tatcgggcta ctcttatgga
     7141 ggccggataa gacgcggcca gcgtcgcatc cggcaatccc gaataagatg tttactcttg
     7201 cacccggcaa ttcaacattt cattatttta ataaccgcac ccggcacgtt tttcctttaa
     7261 tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac agcctgacgg accgcgacat
     7321 agacatgcgc cggatgcacg gcgattttgc caatatctgc gccatcaagc ccgatatctc
     7381 ctgtcagtgc acctaataca tcacccgggc gcattttggc ttttttcccg ccatcgatac
     7441 acaacgttgc catttctgct tccagcgtca caatggaact attagctggc ggcgtttgcc
     7501 agttaagttt tatctgcaac atgtcagaaa tgatattggc ccgctgtgct tcttccggag
     7561 cacagaaact gatcgccaga ccgctatttc ctgcacgagc tgtacgaccg atgcgatgta
     7621 catgaacttc agggtcccac gccagctcaa agttcaccac cagctcaagc gatttaatat
     7681 ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg ggcgctaccg ttagcaaaac
     7741 gtaccagggt ctgatcgcgt tgctccagat cgccgtgtaa cgacaatgca ctttgctcta
     7801 cttcattcag cgcgtcgcag acagcctggc aatctttttt ggtattgcaa aacaccacac
     7861 aagaggatgg ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttactgg
     7921 atgtctcata aaattgttgt tcaatgggtg gcaaagcatc tgttgagtca atttcaatcg
     7981 ccaaaggatc gcgttgcact cgtccgctga ttgcggcgat ggcttccggc caggttgccg
     8041 aaaacagaag cgtctgtcga gatgcaggcg caaaacggat gacatcatca atggcatcgc
     8101 taaatcccat atccagcatg cggtcggcct catccatcac caaagtattc agcgcatcca
     8161 gtgataccgt gcctttttgc aagtgatcca gcaaacgacc cggcgttgcc acgataatat
     8221 gcggcgcatg ttgcagcgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca
     8281 aaattttggt atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg
     8341 ccagttcacg cgtaggacac agcactaaag cctgggtttg aaatagcgac gcatcaattt
     8401 gctgtaacaa gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc
     8461 gaacatcttt tccggcaagg atcgccggaa gcgcggcggc ctgcaccggc gtcatggtta
     8521 aataacccaa ctcattaagg ttcgtgagtt gggcgggagg caaaacattc agggtagaaa
     8581 aagcggtcac aatctattct cgtggtcatc gacgcaaagt tagcaggcgc gtatcctcgc
     8641 agatctacgc tcacgatgcg acaatttaat cggttcttca tcgggtggtg ggtcaggcat
     8701 gggttgcggg cgagggatcg gatcgggcac tggaacagga tcgccaggaa tcggttcagg
     8761 gacaggaatt tgcaaataaa taagtgtcgt catatttccc tccggtcatt gggtggactc
     8821 ttaaagggta gacgctgata aataacaggc aaaaaaaagc cgactcatca aagtcggcgt
     8881 cgtacgaatc aattgtgcta tgcagtaatt caaaaaagga agtaagacaa tatggagcgc
     8941 aacgcccatc gcttgacgtt gcattcacct gcaagggaga tattgccctg aatgggtaga
     9001 gagtttattg acttcgctca aactttgcgg cgtttttgta tacagacagc cggaaaaatt
     9061 gcttttgtta caaccattta ctacggtgca accataaagc aacaccacca ataagaacaa
     9121 ctaacagaat acaaaaaatt gaaaatccga attgccaccc gccgccaggg atcccaccaa
     9181 ggttgacgcc aaataacccg gtcagaaagg tactgggtaa aaagaccatt gccatcaacg
     9241 acattgtata ggtacgacga gctaaatttt cctgcatcac ctgagcgatt tcatccgcca
     9301 tcacgccagt ccgtgctata caggcgtcga tttcgtcaag gccgcgccca aggcgatcgg
     9361 caatatcctg catccgacgg cgttggtcat cgctcatcca cggcaaacgt tcactggcaa
     9421 gacgagcata aacatcacgt tgcggtgcca tatagcgacg catcacaatt aattgtttgc
     9481 gcagcagagc caggaatcca cgcggtggaa tttgctgatc aaggagatta tcttcaaggt
     9541 cgataatttt atcgtgcagc tgctcgataa attcactgga atgatcggtc aacgcatcgc
     9601 acacatccac cagccatccc ccgcaatcgg tcggacccgt gccctcttcc agatcgctca
     9661 ccacatcgtc cagcgccagc actttgcgtt gtcgggtcga aacaattaac cgcccgtcca
     9721 tatatacacg catggcgacc agttgatcgg ggcgttcatc ggtgctgccg tttatacagc
     9781 gcaatgtaat cagcgtgcct tcaccgaaac ggctgactcg gggccgtgtg ctctcgcccg
     9841 ccagcgcatc acgtacgtta ttgggaagca gcggtgttgt cgccagccat tgggcgctat
     9901 catggtgtac ataattaagg tggagccagc agggatgcgc ttcatcaatc acatctgtat
     9961 tttccagcgg tttaacgccg cctctaccat ccagcatcca ggcaaatact gcatccggga
    10021 cattaacgtc cgatccctta atcgcttcca cagtgcctcc atcatcaacg cattattttg
    10081 tagtctagcc ttctggccct gttacgcaac atctcatcac cccattaccc tgaaatgatt
    10141 aataaaattc tgtctaaatt gaatacaaaa agcaaaatgc ttttccgtat acaaaccgtg
    10201 tgaagtgtta aatagcgtct atcattatca gaattatctg atcatatgac gtggcttttt
    10261 tgcgatcgga tagcaacaaa aattgataaa aataacggga tctcaatgat tacgcacaac
    10321 ttcaataccc tggacttact caccagtcct gtctggattg tttcgccctt tgaggaacag
    10381 ttaatttatg ccaatagcgc ggcgcgactg ttgatgcaag acctcacgtt tagtcagcta
    10441 cgaaccggac cctattccgt ctcctcacaa aaagaactgc cgaaatacct ctccgatctg
    10501 caaaaccaac acgatattat cgaaatcctc actgttcagc gtaaagaaga ggaaacagca
    10561 ttaagctgtc ggcttgtttt gcgagagctg acagaaacag aaccggtgat tattttcgaa
    10621 ggtatcgaag cgccggcaac gctgggttta aaagccagtc gctcggcaaa ttatcagcgc
    10681 aaaaaacaag gtttttatgc gcgctttttt ctgactaact ctgcaccaat gttgttgatt
    10741 gacccgtcac gagatggaca aatcgtcgat gctaacctcg ccgcgctcaa tttctatggt
    10801 tataaccatg aaacgatgtg ccagaaacat acctaggaaa taaatatgct tgggcgtcgc
    10861 gtcatgccta tcatgcatga aatttcgcat ttacccggcg gtcataaccc tttgaatttt
    10921 gttcataaac tggcggatgg atctactcgt catgtgcaga cctatgccgg accgattgaa
    10981 atttatggcg acaagctcat gttatgtatt gtgcatgata ttactgagca aaaacggctg
    11041 gaggagcagc tggaacatgc tgctcaccat gacgcgatga ccggattact gaatcggcga
    11101 cagttttatc acattacgga accaggccaa atgcagcatc tcgccatcgc tcaggattac
    11161 agcttgttgc tcatcgacac cgatcgtttt aaacacatta acgatctcta tgggcattct
    11221 aaaggtgatg aggtgttatg cgccctcgcc cgccccctcg aaagttgcgc tcgcaaaggc
    11281 gatttggtgt ttcgttgggg aggcgaagag tttgtcttat tgctaccaag aaccccactg
    11341 gataccgcgc tttcgctggc tgaaactatc cgcgtaagcg tggcaaaagt gagtatttcg
    11401 ggcttaccac gctttaccgt cagcattggt gtggcgcatc acgaaggaaa tgaaagcatc
    11461 gatgaactgt ttaaacgcgt tgatgatgct ttgtatcggg cgaaaaatga tggacgcaac
    11521 cgcgtgctgg cggcataagc cgcggatgcg tctcgagatc aacgactgcg cttagcgtgg
    11581 cgctcccagt tttcttgctt cgcctgcgcc gttttacgta gtgcgacgta acacgccccg
    11641 ctgccgccat gatgcggtag cgcggtgcaa tatgcctgaa catcatcaaa ttcggtcagc
    11701 cagcgcgcca catagctgcg gacaatattg gcatgtgatt tatcttcccg ccctttacca
    11761 tgaataatca gcacattacg caaaccatcc gccagggctt gttgaatgaa actgaacacc
    11821 attttgcggc actcttccac cggctggcgc aaaagattca ggctcgcctg ttgcggatat
    11881 ttaccactgc gcagcttatc cagcacccca tgctgcaacc cttcctgccg aaactccagc
    11941 ggctgactta gcgggatgat gtcgagaaat ccggtagtga ggaaattatc aagctgcagc
    12001 gtgtcgatac gctgcggcgc acgttggtta cgcgttggat gccagtggac atctgtagca
    12061 cgtttcagcg gctggacatc ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc
    12121 atgtaacctc ctatgcacct tcacacgtcg tgattcagac gggttaattg ttcatactta
    12181 ctgcatcgtt cccttcacag attgaaacaa tactctactt catacctgtg agtatctgcg
    12241 caggcggggc attttatact agcctgttag cagtatcgaa cagtttagcc cgagtttaat
    12301 tcagcacagg ctccgatcag gatgatttat tcatagcagg aaccaacaaa aatgcgataa
    12361 ataacagacc actgattagc agaaaggtac cgaaaatgcc atagtttgcg cctccctcag
    12421 catcaattga ctcaataatt tggttgaagc cagtatttcc gatgtctggt cacttctact
    12481 gcgattaaaa aataaagcac ttctggaaat gaagtcattc accgtatggg gaacagaaac
    12541 cattttttca cccattctcg aaaaatgaaa attagttcga ttattgagaa tagagccaca
    12601 cagggcaata ccaatcgcgc ccccaagatt acgtgttaaa ttaaatacac cagaggctaa
    12661 cttcagccgt tcttttggaa ttccccccag cgttaatgtt acgatggggg ccatcgcaaa
    12721 ctgctgcgaa atccctctaa ttgcctgtgg aaacagtaat tcctgccatc cccattcgtg
    12781 ggttataggg gtaaaaagat acattgaaaa cacaaatccg cccagccccg ccatcaacag
    12841 ccatcgcaga tttatttttt tacttaacca gaagtaaaag ggaacagaaa ataactggaa
    12901 aatacctgtt gtgcaaacgg caaaacctat ctcttcggca tttaatccgc gaatctgccc
    12961 caaaaacaca ggtattaaat atacagtaga gaatatccca acccctccag aaaaagaaaa
    13021 aaaacatccc agagtgaaat ttttatcctt aaaagcatga agatccatta tcggattgga
    13081 tattgtaagt gtacgcgcag caaataaaat aaaactcacc aatgccagaa ctgatgtcag
    13141 caaaatagta ttatcatcca gccatcccca gcgcgctcct tcttcaaggg tatattccag
    13201 acatcccaga gtcatggcca acaggataat actcggataa tcagcaactt tcagtaaaga
    13261 gagatcaggt ttatcaaaat taaccagaaa aggaatactc aaaactaaat atatgccagg
    13321 taatatattg atataaaata gccatcgcca gtcgaggtta tcagtgagcc agccccccag
    13381 agttggccct agcgtcggtg acagtgaggc cagcgcactg actaccgcag cagccaaccc
    13441 caactcttta ccctggtaat aaataaacgc catcgtgaaa actaaaggga tcatggatgc
    13501 gccagcagct ccctgtaaag cacgaaacaa aatcatgatc tgaatattcc atgcaagtcc
    13561 acacgcgata ctcattaaag taaatattcc cgcggataga gtaaaaagcc aacgagtgga
    13621 aaatacccgt gacagccacc cagagagcgg aataacaata atctcagcaa ttaaatagct
    13681 ggtctgaagc caggccattt cgtctttacc tgcggacaga ccaccaccta tttcatttag
    13741 ggatgacgat acaatttgaa tatcaagctg agcaataaat aatcccatac acatactggc
    13801 gaaagcaata tgtttacgca tagacatcat ctctcatcaa cactgacggt gacggacagt
    13861 cccgggcgaa gaagttgctt taattccttt gcgtcgtcaa atgcaatccg taccgggaca
    13921 cgctgaacaa ttttggtgaa attgccagtg gcattttcta tcggaatcaa gctgaaactg
    13981 gctcctgtcg ctggggaaag gctttcaatg tgaccgtgaa acacttcccc tttgagaata
    14041 tcagcacgga tctcagcctt cattcctggc ttcattcccg ctatttggtt ttctttataa
    14101 tttgcatcaa cccacaactc actgactggt actagtgaaa ctaacgatgt gccgccttcc
    14161 acccaactgc ctgtatgggc gctgcggttt gcgacaatgc catcaatcgg cgcacgaacc
    14221 acggcagcct gacggctgca aattgaaaac
//
LOCUS       DAJTUN010000104        14050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTUN010000104 DAJTUN010000000
VERSION     DAJTUN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.104"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..363)
                     /gene="wcaD"
                     /locus_tag="OO470_002994"
     CDS             complement(<1..363)
                     /gene="wcaD"
                     /locus_tag="OO470_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6252180.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLV"
     gene            complement(338..1555)
                     /gene="wcaC"
                     /locus_tag="OO470_002995"
     CDS             complement(338..1555)
                     /gene="wcaC"
                     /locus_tag="OO470_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6252181.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1552..2040)
                     /gene="wcaB"
                     /locus_tag="OO470_002996"
     CDS             complement(1552..2040)
                     /gene="wcaB"
                     /locus_tag="OO470_002996"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6252182.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2043..2882)
                     /gene="wcaA"
                     /locus_tag="OO470_002997"
     CDS             complement(2043..2882)
                     /gene="wcaA"
                     /locus_tag="OO470_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6252183.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(2975..5137)
                     /gene="wzc"
                     /locus_tag="OO470_002998"
     CDS             complement(2975..5137)
                     /gene="wzc"
                     /locus_tag="OO470_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6252184.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5140..5583)
                     /gene="wzb"
                     /locus_tag="OO470_002999"
     CDS             complement(5140..5583)
                     /gene="wzb"
                     /locus_tag="OO470_002999"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6252185.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5589..6728)
                     /gene="wza"
                     /locus_tag="OO470_003000"
     CDS             complement(5589..6728)
                     /gene="wza"
                     /locus_tag="OO470_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6252186.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7037..7186
                     /locus_tag="OO470_003001"
     CDS             7037..7186
                     /locus_tag="OO470_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252187.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7387..8970
                     /locus_tag="OO470_003002"
     CDS             7387..8970
                     /locus_tag="OO470_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6252188.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9422..11275)
                     /gene="asmA"
                     /locus_tag="OO470_003003"
     CDS             complement(9422..11275)
                     /gene="asmA"
                     /locus_tag="OO470_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6252189.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11297..11878)
                     /gene="dcd"
                     /locus_tag="OO470_003004"
     CDS             complement(11297..11878)
                     /gene="dcd"
                     /locus_tag="OO470_003004"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6252190.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11970..12611)
                     /gene="udk"
                     /locus_tag="OO470_003005"
     CDS             complement(11970..12611)
                     /gene="udk"
                     /locus_tag="OO470_003005"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6252191.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12929..13348
                     /locus_tag="OO470_003006"
                     /pseudo
     CDS             12929..13348
                     /locus_tag="OO470_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(13373..>14050)
                     /locus_tag="OO470_003007"
     CDS             complement(13373..>14050)
                     /locus_tag="OO470_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6252192.1"
                     /translation="LGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGK
                     RLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLY
                     PVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRG
                     NKALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT         3193 a         3719 c         3633 g         3505 t
ORIGIN      
        1 caccagataa aagaaacgta atatcttacg atggttgtgt ggcgaaattg ttttaaaacg
       61 aatactccac accatgccaa taatgatcac aatatagaca aacagcatag ttgaagtgac
      121 gtatttaccg gcatccaacg actgaccaaa caggtagtta aacgccgtga gtcccgcgcc
      181 aatccctaag gcaatcatca attttttaat gctgatgcgt tctaaaaaca gcagtaacaa
      241 gacaggtaaa aaagtgacga tggtgatggg gaaactttcg ccaagctggg cgattttgac
      301 gttaaccagc aggtagatca gtggcagcag caggtagcta cagattctga tagaagttga
      361 catactcctc cagcatctgt tgtccactgt aggcggcgcg actgcgttgg ctgaactcag
      421 ccagcgtggt accaaatatc gcctgcgcga tttccggttt gcttaactgt accagttgca
      481 gcacctcttc ttcactgacg gttttaccac cggatttttg cagcacttcc cgcgccgcat
      541 cgctatgggt ggcaatcacc ggcacgccaa tcgatagcgc ctcacacaaa atcagcgggt
      601 agttatcgac acgagaactg aacaccagcg catccatctg attgagcgcg ctcatcaact
      661 tgcgcttgtc agtttcaaag ccgtgattaa ccacgttgcc agcggtgaac ggcgagaact
      721 taccaaaggt atgcagttcg attttgtcgc ccagcgccat catctcacgc accagttgct
      781 ggttagtttt gccgtcgtaa cgcaggtcat gcgccaccac tgcgattttc ggcttgccct
      841 gagtttcgcg caccggaggc agatctgcca gaatcgcttc ggttgccata tcaatgccat
      901 tattgataat ccggcaacgc cctggcccgt acagactatt gaaagcgtca gccacatgct
      961 ggctggggga aataaactga cagcccagcg ccagtatctc gcggaataac tggcgtttgc
     1021 ccgccaccag ctggtgcgcg cgatcaatct tcaccggcgg atagttattt aaggtcgggc
     1081 atttctggca gcccgttttc cagccttcgc aaccgtcggt aaaggcgcag cgtccggtta
     1141 cactccagtg gtcgtgcagc gtccagacca gggtgacatc cggtttatgg tttttcactt
     1201 tttcgcaaaa gcgcaccacg ctgttaagat tcaaccagta gctgtgcagc acatgaaaat
     1261 gcaggaccac cggacccggt gtgcgagtaa tggtgcgata taactcattg aaattgccga
     1321 acagatcgcg attaaacaga cgaaacaacg caatgttcgc catcgcggtc atccgcggcg
     1381 tatgtttgat gacctgcgga tagttctgat ggctgacgct ctctttgccg cctttgccgt
     1441 agccgtagac aaaatgtgac gccagcccct gttgcagcgc acgctggtgg agatctaacg
     1501 ccacacctgc tgccccgcct tccgccagtc gcacattaaa ttgcagaata ttcatttaat
     1561 caccttcact cgcgcttttt ctcccaccac cagcgcgttg tccgggacag agtcgagcac
     1621 cacgctgccc gcacccacgg tgacgttgtt accaagcgtg atatcaccaa gaataatgac
     1681 gttggcaccg agttcgacgc cgttgccaat gtgtggacat gccatgttat cggcaccccg
     1741 attgccgata gtgacgccgt ggcgaatggt gaaatcatcc cccgccacca cgtttttatt
     1801 gatcactacg gcgtaaccgt gatggatagt aaagcggcgg ccaatggtcg cggcggcctg
     1861 gatttcataa ccgaaaaagc attcggtgat aatgcgatac agcaccagca gcggggccgc
     1921 ccacagattg ttgaggacgt tctttttgcg ccacaccgaa caaaaatgag cgacgcgata
     1981 ggcaagtacc atgcagcacg ggcgtaaact ccagctgttg gcgcgcagat cttccagcat
     2041 ctttagcgcc cccgaatacc atcagccaga cgtttgccgt tacgcaccga cagcagcgtc
     2101 aacaaggtgc gccaggtcat acgtttattg cggatctggt agagggtaaa cagctgatat
     2161 tttttactgg cgcggtcgaa tttgtctttg tgcttgcggt aaaagtggaa gtatccggag
     2221 aattttttcg gtgacgaggt gatctgcatt tcgccgtgat tgatatgcag gatctgcgtc
     2281 gcctcttcca ctttccacgg ttcgccgtac tccaccacca tccgcaggaa tatgtcgtaa
     2341 tcctgtgctg ctttcagttc ggtatcgaac aaacactctt tgaaacgcca tgcccaggta
     2401 aagacctgat tgccaatgat attacgttta tagaacaggc ggcgtgagta cggcgatttg
     2461 ggatacagtg gtaagctcgc cggttgggaa taaacttctc cctggcaaac gtagtcgtta
     2521 gcgtacaaaa aggcgtgtgt gaccagttgc tgtttatggg cgaggaagac gctcaggcgg
     2581 ttgggcgtcc attcatcatc atcatcgatc ccggtgatgt attccccttg cgccagcata
     2641 atcgcctggt tacgcaccgc gcacgccccg ctgttaatgt cgttgtggat gtaagtgata
     2701 cgcggatcgt tgagggcagt gacgtactgt tgcagttgtt cccaggaggt ggagcaatca
     2761 tccacgatga tcatctccca gttgctgtag tcctggcgca ggaccgattt tatcgcccga
     2821 atcgccagtt gttggcggtt ccaggttggc atatagattg agatcagcgg attgtctttc
     2881 ataggttgtt ctcccggctt atgagctggt ttgcttgccg gatgcggcgt gagcgcctta
     2941 tccggcctac ggggcggtgc gaatgcaggc gtcgttattt cgcatccgac ttatattcgt
     3001 attcgtaata gccataatcc tgatacgcgc tggcgcggcg gaagatggag ttcagaatca
     3061 cccctttcac cggaataccg ttttgctcaa agcggctcag actggtttcc acttctttca
     3121 atgtgttgac cgcataacgc gccaccatta acgtggttcc gacatgacga ccaacaattg
     3181 ccgcatcggt cactgccaga atcggcggcg tatcaatcaa caccaggtca tagtttttac
     3241 tcgcccagtt caccagttcg gcaaagcgtt cgctcattaa cagttcagaa ggatttggcg
     3301 gcacctgacc gcgcgggatc aggtcaaatt tggcaataga ggtcggttta gcagcggtag
     3361 taatatcgcc ctgaccaatc agaatctccg acagcccatt gacattattg gtgcccaaca
     3421 gctcgtgggt gtagcctttg cgcatatcgc agtcgatcaa caacacgcgt ttattggtct
     3481 ggctgatcac cgccgccagg ttggcgcaga caaaggtttt accgattgac gggctaaccc
     3541 cggtcatcat caacacattg ttctgcgcct gcatcatcgc gaagtgcaga ctggtacgca
     3601 gactacggat ggcttcaatc gccagatcgg ttggattccc cactgccagt agctggctct
     3661 gtttatagcg tttaacccct ttgatggttt tgacgctatc gcgcgctttc tgccactccg
     3721 acagcgggat gctggcatag acgctgattc cgtgttcttc cagcacttgc gggctttcga
     3781 tgccgcgatt aaacaacgag cgcagcagca cacccacgat agagagcatc aggccaagga
     3841 taatcgcgcc gaggataatc agccctttct tcggttttag cacgccaggc tgagtgattg
     3901 ccgggtcaat aatgcgcaca tcgccgacgg tgctggcttc ggtgattttc agctcctgct
     3961 ctttattcag cagttgcata tagacctgct gaccagactc gacatcgcgg gtcagacgga
     4021 caatttcctg ctggtttttc ggcatcgccg ttacgcgacc gttaagtttg gctttctctt
     4081 cttccagcgc ctgacgtttc tccagcagcg tgcggtacgc cgggtgaact ttggtgtaca
     4141 gcttggagat ttccgcctct ttaaaggtca gttcgttcaa ctgcgcgtcg atgttcacca
     4201 tcgaatcgag caccgctttc gcttcaagcg gcagatcaac agaatcttta tcctgacgga
     4261 aggcattcag tttgttttcg gcaacatcga ggcggctacg tacttccggt aactgttgcg
     4321 cgaggaaagc gaggcttttc gacgcttccg ccgatttgcg ctcaatattt tgttcctgat
     4381 agttacgagc gatgctgtta agaatgtcgc ggatctgttc gcgatcttca ccggtataag
     4441 tcaggctcag tacgcctgcg tctttgccgt tctccgttac cgtcaggctg ttttgcagtt
     4501 ggttgatcat ccccaacgtg gagtatttgg tgacggtaaa ctcactgccc gggcgggcgt
     4561 gaatggcttc aaccatcagc gtgacgcctt cttttttcag catctggccc gcttgcccac
     4621 gggcgctaaa gccgccatca ctgctcaggg tgtagttttt gtcatccagc acattaagcg
     4681 taaacacctg atccgccatc tctttcgggc ggttaaaggt agtcactttc accgtctcgt
     4741 tctgacgccc catcaggcga tcccagcccg ccccgaaaat cgggaacgtg tttttgctca
     4801 ccgcaatatc gaggtcgaga tcgtccaccg ttttaccaag caccagacgc gaacgaatca
     4861 actggatctc ggcgtccgat gcaggcggtt tgttggctaa tgccgagccg atatcctgca
     4921 ccagcgaatt gccgctgttt tgctcgattt gtaccagtgc gtcggcacta taaatcggcg
     4981 tggcgaagaa agtgtaaacc acggcacaga gggcaaatac gacggtgatg ccaatcaccc
     5041 accagcgcgc ttcaatgacg gtgccgacca ggcgaccaat atcgatttca tcactgcccg
     5101 ttaccggagc ggcatgttgt tttacttttt ctgtcattct tatacctgct ctgcgttcaa
     5161 tgcctgcgcc cactggcggg cagaccgttc aagtaatgtg tacaccgctg caaacgtttc
     5221 ccggcttttg cgatacggat cggggatttc acattcgtta tcccagtgac caaacagcat
     5281 cactttgccg cgcatctccg gtgccatctc gcataagcgt tcgatatggc gcttatccat
     5341 ggtcaaaatc aggtcatagt tgcgacacag gcggcggctg atttgtcgtg cacagtggcc
     5401 ttccagagac agttgatgtt ctgcggcgac gctgatagcg gtaggatcag cgcccttacc
     5461 gaccagcgcg ccgagtccag cggactccac tttcagttcc gggtgataac gttgcagtaa
     5521 gcgttccgcc atcggggaac ggcaaatatt gccgacacag acaactaaga tgttgttaaa
     5581 catgacgatt accagttatg aatgtcgctg gctgtatccg tcatgtaacg gacaccgcta
     5641 atagttggca gcaactgatt gatcagacgg ttccagcggg aaaccggggc ggtggtgaca
     5701 tacaccacgt cataaggttg caggcggaat tctgtcgcca tcaccagcga cgtcgcatcg
     5761 gacatatcca gctggtagat attggcaatc ttgccgttac gcccgccctc gcctttcagc
     5821 ggacgaatga caaagatgcc gctggcgttg gaggtggtca tgtcgatacc ttcggcattg
     5881 gccagagctt cagtcagggt catgccgcta aagtccattt tcagggtgct ctgtttcttc
     5941 acttcaccca tcacaaatac tttcagatca tcattacgcg gcacgtagag aatatcgccg
     6001 ggataaagca gtcggttctg attgaggtcg ccgttttgca tcagcgcctg taaagaaatg
     6061 cgttcttcac gaccattgtg tgttagcacc acgttgcgcc agtcagcggt gtcggtcagg
     6121 ccacctgcgg cgttgatggc gtcgagaatg gtcagtggta cgttggtgat cgcctgttga
     6181 ccggatttat tcacctgacc tgagatatag gccttttgtg agcggaaggc ggcgatatta
     6241 acgtccacct gcgggtcagc gatgtacgtc gctaagcgcc cggtaatatc actgcggatt
     6301 tcagcgagcg ttttcccgac tacgtgaacc ttgccgatat acgggtaaaa catagtgccg
     6361 tcaggctgta cccagttgcc ggtgtcgctg gagctgcggt actgaccagc tggcgtggtg
     6421 agttccgggt gatcccagac ggtgacatta agaacgtccc ccggcccgac gcgatactgg
     6481 taattcgcga tctcgctttc cagcgtcata ttggggcgcg ctacattcgg gcgtgggcgt
     6541 aattggtcaa tcaggcgcgg ggtcagcgga taaacattca ccattttgtc gagatcgaaa
     6601 tcagcgtcct gctgtttgat gacgtctttg cccatcgtcg acatattgct gcccggaagt
     6661 actgtgcaac cgcttatcaa ggttactgac accaataatg gcatcaattt cattttggat
     6721 ttcatcattg tttatttatc actttggcag agtaattatc ctgtgcacta ttaatagcaa
     6781 tgtcgccatg cacatttacc ttgcagttaa ttgaataaaa atttaactgg catcagtcct
     6841 aaaaaaattg atttcatccg caggctattg acagaataat tcagactggt ctttcaggca
     6901 tccagacacg ctaccgcccc tggcttttta gctaccaata cactgattga gcttaatttt
     6961 tcaaaccctc tctgcataca atcgttgatg agaaagggtt attgcggaaa ttaacttccg
     7021 aatataaggt gacattatgg taattgaata ttggctcgcc aataatgcag gaggaagtgt
     7081 tacagctaac ggaatagcag gcaaggtaac aattcggcaa ttggctattt ttaagaatta
     7141 tattaagtat cattcaatat ggtttttagg agtttcttta ggttgacaat atttaatatt
     7201 gcgtctccac atgcgatatt tcttaaataa tgttttatta ttaccgcttt taattcaggg
     7261 ataatgtgag gttattaccc tcaaataata tgagataaat agtgctgcaa cattgcattt
     7321 ttgccccgat ttatccatga tcgaattgtg acatttgtca tacaacgaat aggttttgta
     7381 cttactatgg aatggattgc cgatccgtct atctgggccg ggttaatcac gctgattgtg
     7441 atcgaactgg tcctcggcat tgataacctg gtctttattg ccatcctcgc cgaaaaacta
     7501 ccgccgaagc agcgcgaccg cgcacgggtt accgggctgc tgctggcgat gttaatgcgc
     7561 ctgttactgc tagcgtcaat ctcctggctg gtcaccctga ctcaaccgct gttcagcttc
     7621 cgctcgttta cctttagcgc ccgcgactta atcatgctgt ttggtggttt cttcctgttg
     7681 ttcaaagcca cgatggagct gaacgaacgg ctggaaggga aagacagcaa taatcccaca
     7741 caacgcaaag gtgcgaagtt ctggggcgtg gtgacgcaaa ttgtggtact ggacgccatt
     7801 ttctcactcg actcagtgat taccgccgtc gggatggtcg accatttact ggtcatgatg
     7861 gccgccgtgg ttatcgctat cagcctgatg ttgatggcca gcaagccgtt aacgcaattt
     7921 gttaacagtc acccgaccat cgttattctc tgcttaagct tcctgttgat gattggcttt
     7981 agcctggtgg cagaaggttt cggcttcgtc attccgaaag gctacctgta cgctgccatt
     8041 ggtttctcgg tgatgatcga ggcactcaat cagttggcta tctttaaccg gcgacgtttt
     8101 ctttccgcta atcagacgct acgccagcgg actaccgaag cggtaatgcg cctgcttagc
     8161 gggcaaaaag aagatgcgga actggacgcc aaaaccgcgt ccatgctggt ggatcatgga
     8221 aaccagcaga tctttaatcc gcaggaacgg cggatgattg agcgggtact taatcttaac
     8281 cagcgtaccg taagcagcat tatgacgtcg cgccacgata ttgagcatat cgatctcaac
     8341 gcgccggaag aagaaatccg ccagttgctg gagcgaaatc agcatacgcg gctggtggtt
     8401 accgacggtg atgacgcaga agatttgctc ggtgttgttc acgttatcga cctgctacaa
     8461 cagtcactgc gcggcgaacc gctcaacctg cgggtgttga ttcgccagcc gctggtgttc
     8521 ccggaaacct tgccgttgtt accagccctg gagcagttcc gtaatgcccg cacgcacttt
     8581 gcttttgtgg tggatgagtt tggctcggtg gaagggattg tgacattaag tgacgtcact
     8641 gaaaccattg ccggtaactt accgaacgaa gtggaagaga tcgacgcccg ccatgatatt
     8701 cagaagaatg cggacggttc ctggacggcg aatggtcata tgccgctgga ggatctggtg
     8761 caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc acaccattgc cgggctgttg
     8821 atggagtatt tgcagcgtat tccaaaacct ggcgaagaag ttcaggtggg ggattatttg
     8881 cttaaaacgt tgcaggtgga aagccatcgc gtgcagaagg tgcagattat accgctgcgt
     8941 aaggatggcg agatggagta cgaggtgtga tggtatcggc cattttaccc aatgctgcgt
     9001 aacgccttat aacttcagca atgaagcgtt gtctgtggta aacggacatc acagcggggg
     9061 tgtgggtccg gctgtaaatc gccgaatcgt ttcctgtagg ctggggcagc ccacatggat
     9121 gtgggctgag ggcgcgtttt acagggatgt tacctcgcgc ccgtacccga tagccgaaag
     9181 gaataagatg agggaaccgc gccagcggcg atttaccgcc gggagcccgg gttgccaggg
     9241 tggtggcgat tgagccaccc tggcacgttc accggcttga gcgttccata gtagcgagga
     9301 acatcaagtg aacggaacag ccttcgatct tacatattca gaatataata gtgccgccgc
     9361 gaacgcctga tgcgacgctt tacgcgtctt atcaggccta caggacaggt cacctggtaa
     9421 cctacatctt ctccagcaac ttcttcacat ctttgccatt gcgggaatct ttattccgct
     9481 cggcccagtc attcaggcga cgtttcgctt cgtcctgtag atgtttgcgc agtaactgat
     9541 ccacttgcag gctgtaattg agttgctgcc agttgccata aacccgcagc ggtactggcg
     9601 tttctttgag gaaatcaatc agtttgcttt ccccgttcca gccacccacg acccgaatat
     9661 caaactgggt gtcgcaggtt tgatctgcca gattcaacat gccttcccct gtcagcgcca
     9721 gcacaggcga ttgaccttgc atgtcgttta acgtcacgac gccatccttc aacgtcaaat
     9781 cggtggtaaa gcggtcaagc cgcgtcacgt tatcgaagtt ttcagcggcc ttcacatcac
     9841 cgccattacg ctctaccgct tgctgaatca tctgctggaa gttcatccct tccatacgtg
     9901 tgtcggtcat ctcaacatgc gcctgtcctt gccagttgtg gcggaatgcg tcggcatcta
     9961 tgtcagcacc ggagaaatca ccagccagtg acatttttcc ggtcaacgaa atcggatagt
    10021 taaacgcctt cagaatggta ccaatctcaa cgttctccag ccgtggctgg aagtttatcc
    10081 gcggatttat tgatgtcgcg tccagcgtgc ccggcagtga aacctttcca ccgttaagct
    10141 tgccctgcag ttgagtaatt tccagcaaac ccgacttgtt ggtcatttgc gtggcaacat
    10201 ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg caacaaaata tcagctgtaa
    10261 agccttgcag tccctgatag gccggttcat caatacgcga agaaatgacc gggcgcggca
    10321 acgttgattg gctctgcccc tgctgcgcgg caccgttttc accattcgcg gtttcattaa
    10381 gcgggatgag gttgtcaaga ttcagttgcg ggaattgcag tctcagctgc cattccggtt
    10441 tctctgtcag cgtgacctgt gcttgtccgc tcagcgtact gtcattggcg gtcaaactaa
    10501 tttggttaaa tgaaaggcgt ttatgtgact cctgccactg ggactggaaa ctcccctgcc
    10561 cctgaatacc ttgttttggt aaatcggcac cctgcaactg ccagttaatt tgttcaatag
    10621 ccgctgttaa atcatgcgga taatcagaag catctaccgt accgttgagg gatatcgtga
    10681 gatcgcgctg atcgcgatta acccgcccgg agaactcaaa tgagccacga tgttggggat
    10741 cttgttccat ttgcaggcgg atattgcgga ttgtcacctg ctcgtcatct tcatgctgga
    10801 acaccagaac gctgtccgcc accttaagac tggatatatc aaacgaccat ccgcgatcgt
    10861 ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg agcgtcttca ctgcgcaccg
    10921 cttccgtctg cggcgtcagt tggatcactg ccccttttag catcacctgc ttaacgctca
    10981 gttgatgact cagtagtggt aaaagcgcca cgtccagacg catgttgtcg gcgcgaacca
    11041 gtggctggct tgcgccctgg gcggtgagag acattcgccc ggagaggata ctaagctgcg
    11101 gccagacgtg ccaacgcagt ggcccgtcga gctgcaattg ataaccgcta cgtgcagcaa
    11161 cttgcttgac catatagtcg cggaaatcat tcggattcac cagcaacact aacgcagata
    11221 acccggccac cagcacgacc aagagtatca tcagcgtcgt cagaaatcgt ctcatggtat
    11281 cctcaatggg cctgaattag tctttatcga ttcggctggc taccgcgccc tgctggttgc
    11341 gatatttcgc atcttcacgg cggttgtaag gtcgcgccgc cgggccggaa agcggctcaa
    11401 agctcagcgc accaattaac atgcccggac gcagcgccag cggcagctta ccggagttgt
    11461 agaactccag cacaatgcaa ccagaccagc ccggatcgat acggtgcgcg gtgacgtgca
    11521 ccatcagccc cagacgcgcc aaagaggaac gcccgtccag ccagcccacc agatcggctg
    11581 gcagcgtcac cgactccagt gtcaccgcca gcgccagctc tcctgggtga agatagaacg
    11641 cctcgccctc gtcgagaacg atctcatcgc tcatcacgcg gtcaagcgcg gcgctcactt
    11701 catctttggg accgctcaga tcgataaacg ctgccgtgtg accacggaag gtacgaaatt
    11761 tattgcccag gcgtacatcc accgtcgcgc cgttaatacg ctccactggc ggacgtgggt
    11821 tgatcgacaa acggccttca tcaagccagg cttcaatatc tcggtcacac agacgcatgg
    11881 cattttctcc tttcgcacat cactccctta acgccaatta cgtcaagggc ataccaactt
    11941 atcactgaac ggtacacaat ttatcaagct tattcaaaga actgacttat tttcgctttc
    12001 aatatatcga tcgcgatgcg gtttttcccg ccgcgcggca cgataatgtc cgcatattgt
    12061 ttagaaggct caatgaattg caggaacatc gggcgcacgg ttttttgata ttgcgccatc
    12121 acggaatcca ttgaacgccc acgctcgtta acgtcacgct tgatgcggcg catcaggcag
    12181 atatccagcg gggtatcaac gaaaatggag aagttaagtt cgtcacgcaa acgcgcatcc
    12241 gtcagcaaca aaatgccttc gagaatgatg accttcttcg gctcaaccgt caccgtttct
    12301 ttcatacgcg tatgttcaac atagctgtaa accggcaggt caattgccga gccgcgtttc
    12361 aacgcttgta aatgctcaag caacaggctg tgatccatcg cgctgggatg gtcgtagttg
    12421 gtcttaacgc gttcttccat cgacagatgg ctttgatctt tgtaatagca gtcttcggga
    12481 attacgccga tgtgttcatc accgacttgc tcacgtaatt cacgataaag ggtactggca
    12541 ataagactct tgccggaagc cgatgcgcca gcgataccga taatgacgca ctgatgagac
    12601 tgatcagtca tatatttagc gacctgatta acctggatgt taggaagggg gcgacgaagc
    12661 gccaaacgcg gcaattatag ggatttcatc cgtctgatac cagtcgaata gcgttgccgc
    12721 gcgctcagag ttaattgttg acaaagaatt cccggtggca aattacgttg atcagtttta
    12781 tgtaaggtaa aaaatgttat acgcagttgc gcaaattatc cgcctttacg tcactttatg
    12841 agcaattcgc atataaaatg taaaactttt gtactagcat aaacacagaa acgaatactg
    12901 gcgacctggc cttgcggata aagcggtaat aagcaaacaa tcacagcatg tattaattgc
    12961 cctgccccac ccgctgcttc acctggtcag tttaggttta gtctcgttta tctttaccct
    13021 tgtctcgctt gagctttcgc agtttggcac ccaactcgcc ccactgtggt tcccgacgtc
    13081 catcatgatg gtggcgtttt atcgccatgc cgggcgcatg tggccgggaa ttgcgctgag
    13141 ctgttcgctg ggaaatatcg ccgcatccat cctgcttttt tccaccagct cgctgaacat
    13201 gacctggacg accatcaata ttgttgaagc cgtggtcggg gcagtgctgc tacgtaaatt
    13261 gctgccgtgg tataacccct tgcaaaatct ggctgactgg ctgcgtctgg cattcggcag
    13321 cgccattgtt ccgcctctgt tggggggtgc ccctattaag ttgtcaagca tgttatgacc
    13381 cctgcggggt ataaaaagtc ccgtcgcgca tcatggcgaa cagaacgtcg cagcgtcgtc
    13441 tcgccagggc gataagcacc tgattgtgtc gttttccctg actcattttg cgggtgtagt
    13501 aagccctgga gagcggatcc ctgagcgcgg cgaaggccga caggaacaac gcccgtttga
    13561 gagctttatt accccgtcgc gagggatgct caccgcgtat tgacgagccg gatcgccgag
    13621 ttaccggcgc aaggccagca taagcagcga gatgtgcggc agaggcgaag gcgcggcagg
    13681 cgacctcggt gaggagtctg gctgcggtcc tgacaccgac tccgggcata ctggtcagga
    13741 ccgggtaaag agggtgagca agaactcgct gttctacctc aagcgccacc tcgtctcttt
    13801 gcttacgcag cgtgatgagc tggagtgcca gacgtggcag tactacggca gcggcattcg
    13861 tgccgggaac gacgacggtt tgttcggcca gtgcctgagc tatgtctgct gcaaggcgtt
    13921 tacccagacg aggcgcaagt ttgcagagct gggctgccag cttcttctca cccagcgaag
    13981 cgagtttttc tggtgaggga tatcgctgga gaagatcgag taccgccggg tgctcaagtc
    14041 tcggaccgag
//
LOCUS       DAJTUN010000105        13951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTUN010000105 DAJTUN010000000
VERSION     DAJTUN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.105"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            291..1544
                     /gene="kch"
                     /locus_tag="OO470_003008"
     CDS             291..1544
                     /gene="kch"
                     /locus_tag="OO470_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6252193.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1599..1772)
                     /locus_tag="OO470_003009"
     CDS             complement(1599..1772)
                     /locus_tag="OO470_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6252194.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1915..3375
                     /gene="cls"
                     /locus_tag="OO470_003010"
     CDS             1915..3375
                     /gene="cls"
                     /locus_tag="OO470_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6252195.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3410..3739
                     /locus_tag="OO470_003011"
     CDS             3410..3739
                     /locus_tag="OO470_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6252196.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3792..4796)
                     /gene="oppF"
                     /locus_tag="OO470_003012"
     CDS             complement(3792..4796)
                     /gene="oppF"
                     /locus_tag="OO470_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6252197.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4793..5806)
                     /gene="oppD"
                     /locus_tag="OO470_003013"
     CDS             complement(4793..5806)
                     /gene="oppD"
                     /locus_tag="OO470_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6252198.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5818..6726)
                     /gene="oppC"
                     /locus_tag="OO470_003014"
     CDS             complement(5818..6726)
                     /gene="oppC"
                     /locus_tag="OO470_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6252199.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6741..7661)
                     /gene="oppB"
                     /locus_tag="OO470_003015"
     CDS             complement(6741..7661)
                     /gene="oppB"
                     /locus_tag="OO470_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6252200.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7747..9378)
                     /gene="oppA"
                     /locus_tag="OO470_003016"
     CDS             complement(7747..9378)
                     /gene="oppA"
                     /locus_tag="OO470_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6252201.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10116..10763)
                     /gene="ychE"
                     /locus_tag="OO470_003017"
     CDS             complement(10116..10763)
                     /gene="ychE"
                     /locus_tag="OO470_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6252202.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11240..13915
                     /gene="adhE"
                     /locus_tag="OO470_003018"
                     /gene_synonym="adhC"
     CDS             11240..13915
                     /gene="adhE"
                     /locus_tag="OO470_003018"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6252203.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3660 a         3453 c         3242 g         3596 t
ORIGIN      
        1 caatatttaa gggcacggtt tttgcaaccg tgttcttgtt tggcctttcc ttatagccgg
       61 tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt atatgatgtg
      121 agcatgttca ggaaaaaagc cctttcctga cgcagagttg ctcactcaaa acctttgatt
      181 actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
      241 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
      301 gggctacatt caaacaaaca gcaacaaatt tatgggtgac cctacggcac gacattctcg
      361 cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca
      421 atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa
      481 acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc
      541 gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc
      601 tgcattttta tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt
      661 tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat
      721 ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag
      781 gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt
      841 caaccgtcgg ctatggagat attgtccctg tttctgaatc agcacgattg ttcactattt
      901 cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc
      961 ttatccgcgg gggattcaac aaacttttaa aaggaaacaa tcatacaatg catcgtaaag
     1021 atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc
     1081 aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg
     1141 agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat
     1201 taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag
     1261 ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg
     1321 ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct
     1381 caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata
     1441 atgatatgct cgtttcaatg ttgttgaact caggtcatgg tattttcagc gataacgatg
     1501 aacaagaaac gaaagctgac agtaaagaat cggcgcaaaa atagagttta aagacggaag
     1561 agtaaactta ttgggcaccg caatggtgcc cagtacattt agatattgta gatcgcaaac
     1621 aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt
     1681 cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc
     1741 cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg
     1801 gtcattatac ccccgccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga
     1861 tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca
     1921 accgtttata cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc
     1981 gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg
     2041 attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat
     2101 ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt
     2161 aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca
     2221 ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacag
     2281 ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc
     2341 cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct
     2401 gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc
     2461 gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa
     2521 gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc
     2581 caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg
     2641 gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg
     2701 cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt
     2761 gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag
     2821 gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga agatctcatt
     2881 caccaggcat tattgactgc ggcttattcg gcgcatgaat atttgatcat gaccacgccc
     2941 tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg
     3001 gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc
     3061 gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta
     3121 ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt
     3181 gatatgcgta gtctgtggct gaatttcgag attaccttgg caatcgacga taaaggtttt
     3241 ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc
     3301 cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc
     3361 agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga
     3421 tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga
     3481 actggctgcc gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga
     3541 gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga
     3601 tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga
     3661 aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca
     3721 tatcatctgg cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc
     3781 ccttattgtt tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc
     3841 tccagaacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa
     3901 acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt
     3961 ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa
     4021 ggatgtagtg gattgtggta gacctcatca taggtcccca gttctactgc atggccgaga
     4081 tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaagtcatg agcaataaaa
     4141 attaatgaca atcccatctc acgttgcagc tgctggagca ggttaaccac ctgcgcctga
     4201 attgacacgt ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata
     4261 agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg
     4321 ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact
     4381 tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg
     4441 gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga
     4501 acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga
     4561 ccgtcggtcg ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc
     4621 gattccccta ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg
     4681 gctttgagcg ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc
     4741 actttaagat cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca
     4801 gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg
     4861 gtgcgctact acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg
     4921 gtaatcgcag caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat
     4981 caagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga
     5041 catcgcgagc attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac
     5101 agatccccgc caccacgcca agatcgtggg tgatcataat gatggcagta ttaaattccc
     5161 gcttcagttc attcaatagc gtcatgatct gcgcctgtac ggtgacgtcc aacgctgtag
     5221 tgggttcatc cgcaatcagc aacttaggtc gacatagcaa tgccatcgca atcatgactc
     5281 gctgacgcat gccgccagaa aattcgtgcg ggtacatttt catgcgttta cgtgcttccg
     5341 gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt
     5401 tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag ggattcaacg
     5461 aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcac
     5521 gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca atgcgtccgt
     5581 tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac
     5641 ctacaatgcc cagcgtttct ccggcacgta gggaaaaatt taaatcattg accgccgtga
     5701 cgtcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag
     5761 cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta
     5821 acgatctttc gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag
     5881 tgtcaccaca aggaatcccg ctgggaacaa cagtaaccat ggagagactt ccatcgagtt
     5941 cgcgccatca ctcagcaatg ccccccagct gcttaacggc tcttgcgtac ccaaccccag
     6001 gaagctaagg aaagattcaa agaggatcat gctgggcacc agtagcgatg cgtagaccac
     6061 caccacaccg agtacgttcg gcacaatgtg gcgaataaca atgcccggcg tcgatacacc
     6121 gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac
     6181 aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca gcaggatgtt
     6241 ttggccgaaa aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat
     6301 ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc
     6361 ataaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca tgagtgagat
     6421 acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta
     6481 gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc
     6541 aaactgggaa agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag
     6601 actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg
     6661 cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa
     6721 catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca
     6781 tcgacaatgg cattaaacaa aatggttaac gcaccaacca ggatggtcag gcttaacact
     6841 aaggaatagt cacggttcaa tgcgccatta acgaacagtt gcccaatccc cggcaaacca
     6901 taaatggttt cgatgaccat agaaccggta ataatgccga caaatgcggg gcccatatag
     6961 gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg ccgcataggt
     7021 aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat catagagcca
     7081 cgggtaatac gcgcaatact ggcgatatag gcgagtgaca acgccaccat cggcaatatc
     7141 atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca
     7201 aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc ggtcattgcc
     7261 agccccatca cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca
     7321 ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc agaaacggga
     7381 aaactggatg ccaccaggtc attgaccgaa taatctttat atttaaacga tggaccgaaa
     7441 tcgccatgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg atcattaaga
     7501 tgatatttcg cttcgatatt ggccatcact tctggcggta aagtacgttc gccggtaaaa
     7561 gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat aaatagcgtc
     7621 ggaatcgctt ccagacaacg acgtagaata aattttaaca ttgccctacc ttctggcctg
     7681 tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct gccccacgta
     7741 ttgccattag tgcttcacaa tgtacatatt ccgggtatag gtattatcca gcggatcttt
     7801 gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt aataaacagg
     7861 aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag ctgtgcgctg
     7921 cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg ggctcttata
     7981 atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag ttggttcgtt
     8041 gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac gggtgtcgag
     8101 gaacgttttc cactcctggt taaccagttt gacgtttacc ccaatgtttt tcttccacaa
     8161 tgaagaggca gcaatcgcca gctttttatg cagatcggag gtgttatacc gcaggttgat
     8221 ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg cttcttcgtt
     8281 acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc catcagtata
     8341 cggtggagta taaccatagg cgggcatgtc gccctgcgct ttcactttat taacaatgat
     8401 atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga atggcggttt
     8461 ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa cttcgtccgg
     8521 gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg tcatgtcgat
     8581 ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca aataggttac
     8641 ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa gaacgattcg
     8701 ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat taccaggctg
     8761 ggtccatttt tcgccgaatt tttcgatagc ggcttttggc accggtgaag ttgatgggtg
     8821 aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta atgtgtgatc
     8881 atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa tttcatcaat
     8941 accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt tcggatcaac
     9001 agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgccat cagaccattt
     9061 cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc aggattcagc
     9121 gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa acaggtctcg
     9181 gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact gaacttctga
     9241 accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg gtacatcagc
     9301 tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta ctaaacttct
     9361 cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg tattaaccag
     9421 catgtgtaat cccggattgg gaccctgcac aagctcggag actttctgct gtcaggttac
     9481 tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag acgatcacct
     9541 tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc gacgctcttt
     9601 aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg gaaagtacca
     9661 aatgcggttc ttactcgcca atacattttg caaaattgtt aagcgattct cttttattga
     9721 ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa
     9781 tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg aaatgcacaa
     9841 taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg gaattactaa
     9901 tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa taacatgatg
     9961 gtgaggagaa acagttaagg aaatccttat aactaactgt aatttgagaa gaatattttt
    10021 acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat gacgaagtaa
    10081 acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc ggggaaaatc
    10141 cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag ccccataata
    10201 cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg tgccatgcgg
    10261 aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc aaacagatag
    10321 ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc tggccccgcc
    10381 atcaacggca acgccaatgg caccacacca atactttcac gtaccgcggt ttctgatttt
    10441 tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat cgctatcgtc
    10501 accaccagga tgcccccggc gatacggaac gaatcaattg atataccaaa aagttgtaga
    10561 atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag gttggctgta
    10621 aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat aaagacggga
    10681 ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa gtaaacggga
    10741 aaatcaaaaa gggtctgaat cacggttcgc tccgaagcaa aagccggata atgttagcca
    10801 taaataaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt tcacctccta
    10861 actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta
    10921 gccaccaaat catactacaa tttattaact gttagctata atggcgaaaa gcgatgctga
    10981 aaggtgtcag ctttgcaaaa atttgatttg gatcacgtaa tcagtaccca gaagtgagta
    11041 atcttgctta cgccacctgg aagtgacgca ttagagataa taactctaat gtttaaactc
    11101 ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgattttcat cggttaagca
    11161 aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta aaaaagttta
    11221 acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg cactcgtaga
    11281 gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag tagacaaaat
    11341 cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt
    11401 tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga
    11461 atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg aagacgacac
    11521 ttttggtacc atcactatcg ctgaaccaat cggtattatt tgcggtatcg ttccgaccac
    11581 taacccgact tcaactgcta tcttcaaatc gctgatcagc ctgaagaccc gtaacgccat
    11641 tatcttctcc ccgcacccgc gtgcaaaaga tgcaaccaac aaagcggctg atatcgttct
    11701 gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg atcaaccttc
    11761 tgttgaactg tctaacgcac tgatgcacca cccagacatc aacctgatcc tcgcgactgg
    11821 tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc
    11881 gggtaacact ccagttgtta ttgatgaaac tgctgatatc aaacgtgcag ttgcatctgt
    11941 actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt ctgttgttgt
    12001 tgttgactct gtttatgacg cagtacgtga acgtttcgca acccacggcg gctatctgtt
    12061 gcagggtaaa gagctgaaag ctgttcagga tgttatcctg aaaaatggtg cgctgaacgc
    12121 ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct ctgtaccaga
    12181 aaacaccaag attctgatcg gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca
    12241 tgaaaaactg tccccgactc tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga
    12301 aaaagcagag aaactggttg ctatgggcgg tatcggtcat acctcttgcc tgtacactga
    12361 ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa cagctcgtat
    12421 cctgattaac accccggctt ctcagggtgg tatcggtgac ctgtataact tcaaactcgc
    12481 accttccctg actctgggtt gtggttcctg gggtggtaac tccatctctg aaaacgttgg
    12541 tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca
    12601 caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc tggatgaagt
    12661 gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg
    12721 ttatgctgat cagatcactt ccgtactgaa agcagcaggc gttgaaactg aagtcttctt
    12781 cgaagtagaa gcagacccga ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc
    12841 cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat
    12901 catgtgggtt atgtacgaac atccggaaac tcacttcgaa gagctggcgc tgcgctttat
    12961 ggatatccgt aaacgtatct acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc
    13021 tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg taactgacga
    13081 cgctactggt cagaaatacc cgctggcgga ctatgcgctg accccggata tggcgattgt
    13141 cgacgccaac ctggttatgg acatgccgaa gtccctgtgt gctttcggtg gtctggacgc
    13201 agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct ctgatggtca
    13261 ggctctgcag gcactgaaac tgctgaaaga atatctgcca gcgtcctacc acgaagggtc
    13321 taaaaatccg gtagcgcgtg aacgtgttca cagtgcagca actatcgcgg gtatcgcatt
    13381 tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt cccagttcca
    13441 tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct acaatgcgaa
    13501 cgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg
    13561 ttatgctgaa attgccgacc acctgggtct gagcgcaccg ggcgaccgta ctgctgctaa
    13621 gatcgagaaa ctgctggcat ggctggaaac gctgaaagct gaactgggta ttccgaaatc
    13681 tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata aactgtctga
    13741 agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga tctccgagct
    13801 gaaacagatt ctgctggata cctactacgg tcgtgattat gtagaaggtg aaactgcagc
    13861 gaaaaaagaa gccgctccgg ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt
    13921 agcgctgtct ggcaatataa acggcccctt c
//
LOCUS       DAJTUN010000106        13621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTUN010000106 DAJTUN010000000
VERSION     DAJTUN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.106"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            724..1374
                     /gene="csgD"
                     /locus_tag="OO470_003019"
     CDS             724..1374
                     /gene="csgD"
                     /locus_tag="OO470_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6252204.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1379..1768
                     /gene="csgE"
                     /locus_tag="OO470_003020"
     CDS             1379..1768
                     /gene="csgE"
                     /locus_tag="OO470_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6252205.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1793..2209
                     /gene="csgF"
                     /locus_tag="OO470_003021"
     CDS             1793..2209
                     /gene="csgF"
                     /locus_tag="OO470_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6252206.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2236..2942
                     /locus_tag="OO470_003022"
                     /pseudo
     CDS             2236..2942
                     /locus_tag="OO470_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3075..3566)
                     /locus_tag="OO470_003023"
     CDS             complement(3075..3566)
                     /locus_tag="OO470_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6252207.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3668..4222)
                     /gene="ycdY"
                     /locus_tag="OO470_003024"
     CDS             complement(3668..4222)
                     /gene="ycdY"
                     /locus_tag="OO470_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6252208.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4246..4983)
                     /gene="ycdX"
                     /locus_tag="OO470_003025"
     CDS             complement(4246..4983)
                     /gene="ycdX"
                     /locus_tag="OO470_003025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6252209.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5038..5976)
                     /gene="ghrA"
                     /locus_tag="OO470_003026"
                     /pseudo
     CDS             complement(5038..5976)
                     /gene="ghrA"
                     /locus_tag="OO470_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6211..6298
                     /locus_tag="OO470_003027"
     tRNA            6211..6298
                     /locus_tag="OO470_003027"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6245..6247,aa:Ser,seq:gga)
     gene            complement(6448..6729)
                     /locus_tag="OO470_003028"
                     /pseudo
     CDS             complement(6448..6729)
                     /locus_tag="OO470_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7011..8015)
                     /locus_tag="OO470_003029"
     CDS             complement(7011..8015)
                     /locus_tag="OO470_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6252210.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8087..8647)
                     /locus_tag="OO470_003030"
                     /pseudo
     CDS             complement(8087..8647)
                     /locus_tag="OO470_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8732..8929)
                     /locus_tag="OO470_003031"
     CDS             complement(8732..8929)
                     /locus_tag="OO470_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252211.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8941..9432)
                     /locus_tag="OO470_003032"
     CDS             complement(8941..9432)
                     /locus_tag="OO470_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252212.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9429..9803)
                     /locus_tag="OO470_003033"
     CDS             complement(9429..9803)
                     /locus_tag="OO470_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6252213.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9850..10224)
                     /locus_tag="OO470_003034"
     CDS             complement(9850..10224)
                     /locus_tag="OO470_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6252214.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10387..10608)
                     /locus_tag="OO470_003035"
     CDS             complement(10387..10608)
                     /locus_tag="OO470_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6252215.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10671..11147)
                     /locus_tag="OO470_003036"
     CDS             complement(10671..11147)
                     /locus_tag="OO470_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6252216.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11163..11636)
                     /locus_tag="OO470_003037"
     CDS             complement(11163..11636)
                     /locus_tag="OO470_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6252217.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11978..12799)
                     /locus_tag="OO470_003038"
     CDS             complement(11978..12799)
                     /locus_tag="OO470_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6252218.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12899..13072)
                     /locus_tag="OO470_003039"
     CDS             complement(12899..13072)
                     /locus_tag="OO470_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6252219.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13192..>13621)
                     /locus_tag="OO470_003040"
     CDS             complement(13192..>13621)
                     /locus_tag="OO470_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252220.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3477 a         3329 c         3297 g         3518 t
ORIGIN      
        1 aagaaattaa atcatttcaa cttggttgtt aacgcaacct gtattttgtt aacgctgcgt
       61 tacgatggaa agtatgtctg cggaaatatt tttaataact cacccgcgcg ttgtattttt
      121 ctttttttgt tcataccaaa gtattaaaac ccgcgtaaat acgctgttat ctacgcaaaa
      181 aatattttgt tttctcttaa atctccgttt tccgctatca aaaatcacca gacagtcatt
      241 cttcttgccc gtcgctgatt gctgcgatat gtcttgcgca caagccgtga caaacctcgt
      301 ctttttgcag taaaaaaatt gtccacggag gtgtggagaa aaaacaaaaa cgttttacat
      361 gacgaaagga ctacaccgaa atatttttat atgtattatt agtaagttag caccatttgt
      421 atgatttttt aaaattgtgc aataaaaacc aaatgtacaa cttttctatc atttctaaac
      481 ttaataaaac cttaatgtta acattttaat ataatgagtt acattttgtt acatgtttaa
      541 cacttgattt aagatttgta atggctagat tgaaatcaga tgtaatccat tagttttata
      601 ttttacccat ttagggctga tttattacta cacacagcag tgcaacatct gtcagtactt
      661 ctggtgcttc tattttagag gcagctgtca ggtgtgcgat caataaaaaa gtggggtttc
      721 atcatgttta atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaacct
      781 tctttgcagg cgacagctct cttgcagcac cttaaacaat cgctagcaat tacaggaaaa
      841 ttacataata ttcaacgttc tctggacgat atctcttcag gctctattat tcttctggat
      901 atgatggaag cggataaaaa gcttatccat tattggcagg atactttgag caggaaaaac
      961 aacaatatca aaatattgtt gctaaatacg cctgaagatt acccgtaccg cgacattgaa
     1021 aactggcctc atatcaacgg cgttttttat gccatggagg atcaagaacg tgttgtcaat
     1081 gggttgcaag gcgtcctgcg tggcgaatgc tactttacgc aaaagcttgc cagttacctg
     1141 attacacatt ctggtaacta tcgttataac agcacggaat cagccctcct tactcatcgg
     1201 gaaaaagaga tcctgaataa gctgcgtatc ggcgcgtcta ataacgagat cgctcgttcg
     1261 ttgttcatca gcgaaaatac ggttaaaacg catctttata atcttttcaa gaagatagcc
     1321 gtaaaaaacc ggacacaagc ggtttcctgg gcaaacgata acctcaggcg ataaagccat
     1381 gaaacgttat ttacgctgga ttgtggcggc agaatttctg ttcgccgcag ggaatcttca
     1441 cgccgttgag gtagaagtcc cgggattgtt aactgaccat actgtttcat ctattggcca
     1501 tgatttttac cgagccttta gtgataaatg ggaaagtgac tatacgggta acttaacgat
     1561 taatgaaagg cccagtgcac gatggggaag ctggatcaca ataacggtca atcaggatgt
     1621 tattttccag acttttttat tcccgttgaa aagagacttc gagaaaactg tcgtctttgc
     1681 actgattcaa actgaagaag cactaaatcg tcgccagata aatcaggcgt tattaagtac
     1741 ggacgatttg gcgcatgatg aattctaaat aaaaaattgt tcggaggctg caatgcgtgt
     1801 caaacatgca gtagttctac tcatgcttat ttcgccatta agttgggctg gaaccatgac
     1861 tttccagttc cgtaatccaa actttggtgg taacccaaat aatggcgctt ttttattaaa
     1921 tagcgctcag gcccaaaact cttataaaga tccgagctat aacgatgact ttggtattga
     1981 aacaccctca gcgttagata actttactca ggccatccag tcacaaattt taggtgggct
     2041 actgtcgaat attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga
     2101 tattgctaac cgcgatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac
     2161 ctcgaccatc caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc
     2221 ataaggaaaa taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga
     2281 tgcttaaccg ccccgcctaa agaagccgcc agaccgacat taatgcctcg tgctcagagc
     2341 tacaaagatt tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac
     2401 attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt
     2461 ccgcaaagcg ccagggctta caaaacctgc ttaacgagcg caaggttatt cgtgcggcac
     2521 aagaaaacgg cacggttgcc attaataacc gaatcccgct gcaatcttta acggcggcaa
     2581 atattatggt tgaaggttcg attatcggtt atgaaagcaa cgttaaatcg ggcggtgttg
     2641 gggcaagata ttttgggatc ggtgcagaca cgcaatacca gctcgatcag attgccgtga
     2701 acctgcgtgt cgtcaatgtg agtaccggtg agatcctctc ctcggtgaac accagtaaga
     2761 cgatactttc ctatgaagtt caggccgggg ttttccgctt tattgactac cagcgcttgc
     2821 tggaagggga agtgggttac acctcaaacg aacccgttat gctgtgtctg atgtcggcta
     2881 tcgaaacagg ggtcattttc ctgattaatg atggtatcga ccgtggtctg tgggatttgt
     2941 aaaataaagc agaacggcag aatgacattc tggtgaaata ccgccatatg tcggttccac
     3001 cggaatcctg acagataaaa atgccggaat taataatccg gcatttactg tttaagcgaa
     3061 agcgtgagtg cccctcacgc tttatttttg atttcctgct cacggtgagc cgtctttgca
     3121 ctacgcgccg ccagccacag gccgctgttt ttcattgcgt aaccaaatac cagcccaagc
     3181 gccagcgaag gtaacaccag tttccagtca ccctgcccgg caaacgtcgc gcatgcgcct
     3241 ataaacgtcc ccggaacaaa tgaaagcagc agctgtttgg cctgaataca catcagaaac
     3301 gcgacaatac cggttatgac ataaccgaga atttccagat gtggtgccag tgcactaccg
     3361 taaataatga ccatcgccca caccacgcca ctaagcaggg ttgcagcgga gatcgccagc
     3421 cctttcagcc caccttgcgg gcaagcaaag taggccgtac agcccaggaa gcccgcccag
     3481 cttagtaagc ccagggaaac agccacccat ccccagatac cggagagaat gcctgttgtg
     3541 attgcaatag agagaagtat gttcatgccg cgcatgatag cagaaaacgc ggccatgaat
     3601 gagatcgagc acacatttta tgcaacaaca aattatctgt tgcagttatg agtgacgatg
     3661 atcacattta ctcttcagaa tcttcttcca gctcgtccca cattgcacta atggcatcac
     3721 gggttagcgg tgccatggtg cgccagaaag gcgtggttgc atgggcctcc actttgccga
     3781 ggaacgcacc acaccagggt aacagatact cactgaacag tgtttccagt gcttcgctct
     3841 catcttccgt tgactgatct tccagccagg aagccgcaag cagcaatgtg ccgatgtgat
     3901 cggctggcgt atccgccagt ggcatccctc gttcggaaag aaaagcgcgc acttccgctt
     3961 ccgttgcgtc ctcaacccat gcgctacgat atggcggcac ggcacattca tcgccgataa
     4021 acaacgcatt gtaatcggca gagacttgcg tcatatcaca gcttttctgt aaacgcgtca
     4081 gtaactcatc ctgctccagt ggccagttcg cagccagttt cccctcacga atcagagtaa
     4141 acagcggcac cagtaaagga tcttgcggtt ggcggtaata gagcgaaccc agcacacgac
     4201 agaggataga aaactcgttc attaatatat ttccattact agtaattaaa ggtctgcaaa
     4261 ttccgcaatc ggtgccatac cgcgagattc aaggaagttc agtaagcggc gcggagaaac
     4321 attcaaaatg cgctctagcg gaaaatctac cgcgtcgagg attttaagac actcttcaaa
     4381 ttcccccatg gtaaacgcgg tgtgagaatc cgagcctaat gccacccaac ctccagcatc
     4441 acgtaccgct gcagctaccg cacgacagtt gtcttcactg cccttacgtg agtgtaaaaa
     4501 tgaggaatta ttgatttcca gcgccacctg atgtttcgcg gctgcttcag caacggcttt
     4561 aacatctatt tcatatttcg gatttccggg atggcttatt atatgcacat tgccgctggc
     4621 gatagtggag atcatcgctt gtgtattggt cgctttgtca tgtggcgcaa aaaccggctc
     4681 atgaaaaccg gcaataatca gatccagcga gtcaaacatt ttaccgctgc agtcaatttc
     4741 accatcaaca tttttaatgt tagcttcaat gccgcgcagg atccctaccc catcaaccac
     4801 tcgcggccag atacgcatgt taatgaagtg ccagtgatgc ggcgcatctt ccatatccgg
     4861 gccatgatcg gtgatcgcaa aaagtttaat gcccttttgt ttggcctggg caatgtaatc
     4921 actcaatgtg ctatatgcat gtgtgctggc aacggtatgc atatgaaggt cgacgggata
     4981 cataactctc tcctgtattc aattcccgcc aaggatagca ggaatcctga tgctttatta
     5041 gtagccacgt gcgcggtcga cttgcccgca gaccctctcc cctttttcga gctgggcaat
     5101 agtgcgagaa atgtactcca cagcttcagc gggacgggta atcgcggcga catgtggtgt
     5161 tatcgtcacg cgtggatgtt gccagagcgg actttcaggc ggtaagggtt cacgattaaa
     5221 aacatccagc attgcgcctt taactttccc gctatccagc gccgcgagca ggtcatcttc
     5281 cacaacatga acaccacgcg ccaggttgag gagatacgcg ccatcaggta atttttcgag
     5341 taattgttga ttaataatgc cgacggtttc aggggtattc ggtaacaaat taatcaatac
     5401 ccgacattgg ctcagaaatg cagacagttc ttcccgtccg gcaaagcttt gcacgccagg
     5461 ccacgattta cgggttcgac tccagcaacg cagcggaaag cgctaggttt gcagactctg
     5521 agcaacttta cagcccagta cgcctgcgcc caaaatgccg atggtaaaat cttcccgatg
     5581 atattcaggc agcggttgcc aatgcgaact attttgctgg atgcgataat cgtcaaaacg
     5641 tcgaaaccaa tgcagcacct gactgacagc atattcctgc atttgctcgc ccataccggt
     5701 atcttccagg cgaaaaagtg gaacagaagg gttcagcatt tcagggtgtg cctgtagctt
     5761 gctcaaaata gaatcaacac cggccccgag tgcgaacacc gctttaagat cgcgccctgc
     5821 cagcatttca acaggaggat gccagactaa aacataatca gcagagtcat tatctccgct
     5881 tttccatgcc ctgactcttg cctgaggaat agctttgcgc agtgcctcaa tccaccattg
     5941 ggtatcgaac gttgggtgat aaaagatgat atccatactg actcccgaaa agcgttgtgc
     6001 gaaatttatt cgcacttatc gttatgatct acaaaggcca ccagcataac aaatccgtgg
     6061 tcggtggcaa aaaaagcaga tttcgcttat taaaaccaca cattgattga atgtttgaat
     6121 aaacgcgcga ttttttcaaa aagtttgttg acctcaggtc atgatttccc taaattagcg
     6181 cccgttccag caagacagga acgacaattt ggtgaggtgt ccgagtggct gaaggagcac
     6241 gcctggaaag tgtgtatacg gcaacgtatc gggggttcga atccccccct caccgccaga
     6301 ttataagtgc atgttattgt ttaacatgcg cttttttatt tttccttcca gacagtatgc
     6361 catcgggtat gccatctgaa accggtttat atgccccgcg atcatatctt atcggacaat
     6421 ttccgatctt tgagcatact ggcaaagcta tactgccagc atatcaggat agtgttttgc
     6481 gataatatca ttcagtctgt caaccagttc tggttttctg aacgtgatat gcgcggagcc
     6541 cttcttaaag tatctgatgc tgaacatctc atcttcatag ctctctttac cctgaacaga
     6601 ctgaatgtga tcatccagac gaagggtgat gtttccccgg ttgtcgggga ttggtttacc
     6661 gctgaacagg tgcaacattc tttccagatc ggcgagtcgg tcagcctgtt gtccggtgat
     6721 aagatgaaat ctatggtcac ccccgttttt gcaacactga tttttgatta atggttggct
     6781 tgcttaaatc tatccggcgt ctctgtgggc aaggatgccc gcgccccgat gagttccgca
     6841 cctgataatc ctaaaaatcc cctcggcttc agtgagccat ttcatctgtt aggtttttaa
     6901 tcaggtcgat ttgccgttca tgtcatcaat aatcagtctt cgcaaaacca gaaaggtaac
     6961 ttgtatctta ttgtttaaaa taaactaatt gagttatatc agcatctata tcaggcctga
     7021 tattcttttt tctgagtagt gattgcccag gcaattcgag ccagtttatt tgccagagcg
     7081 caggtaacca cattcgaatg cttcttgttg agttgctccc gaacccactc agcaagtttt
     7141 ccatgcttgc gttcaagtcg cattatgaat gagcgggcac actgaaccag taaccggcgt
     7201 aaatttttat cacctcgctt actgataccc aacagagtcg attttcctcc agtgctatac
     7261 tgccggggaa caagtcctgt ggaagctgca aaatccctgc tgcatgagaa ctgtttcccg
     7321 tcaccaagtt gcgacgatag taagctggca gtaatcggac caacaccagg aattgtcata
     7381 atgcgctgag cggtatcatc agcgttgatt gactgactta attctgattc cagctctgct
     7441 atttgctcaa caaggtaaaa gtagtgggta tgcaacctca ccaaaaggcg gctcagatat
     7501 tcggggattt cgtgttcagc caacacgaga gacaaccgtt ttattacagc atcacctatc
     7561 ggcaggctga ttccaaattc cagcaagaaa ccgtggatct ggttggttgt tttcacctta
     7621 tctctgatga gcgattctct gacccggtgt aacgccctca tggcctgctg ggtctcagtt
     7681 cttggctgga caaaacgcat tgatggtcgt gaagccgctt cgcatatggc ttctgcatca
     7741 acaaaatcat tcttattact tttgacaaac gggcgaacga actgtggaga aatcagctta
     7801 gtttcatgcc caaaatcaga gatacagcga gccataaagt gtgcacccgc acaggcttcc
     7861 attacaacaa ctgagggcgg acatgtcgcc agaaactgca tcagtttagt acgggtaaat
     7921 tttttacgca gaagagcctt accagacttg tcttgacaat gaatgtggaa ggagtgcttt
     7981 ccgagatcga taccaataag tgtgatgttc tgcatgatgg tctctccgtt aaagggattt
     8041 accttaacag cgtactgctg ttaaggtggg ggtgaccata tcattatccc caccggtccc
     8101 atctcaccag attgttgacg ataattttac tgccaaattt acaggggcaa ttggttttgt
     8161 aattccagtt cagccctctg aagacgttga tcacccctcg ctcaaacacc tcatccttat
     8221 tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc gctgatttcc ggaaaattat
     8281 cgtactccag tgaccggtac catgtatcac gggcctgcgt atccattaat gacagcatcc
     8341 cggaacgttg catcaggtca cgccagattt cgcgatcaag attccgggta atggctttca
     8401 ttgctgtttc gggtttctcc atcagccagc agccgcagcg aaaatcctgt ttcatggccc
     8461 agtccggggc agttttacct ccgatgcttt ccgtcagcac cgagatttca gccagttgtc
     8521 ggataagtgt gtcgatttgc aacagtgtac tctttcgtac agccagaatg tggcgaatgc
     8581 tggttgagca gataacgtcc gtatgcccgt tgaccacttc cggacgggag attgtgatat
     8641 ctgacataat cgatattcct ttttgcagat aaatgaaaac gccagccctg tgcaggctgg
     8701 cgtcagagga atgaagtctg gtgagtgagc ttcaccggtt ctggtgcaga agctgcagat
     8761 gacgcagggt acggacatcc tcccgggcct gttcgataca gggcaacagt gctgctgaat
     8821 ccgttttatc ctcatcgttg tcaaagataa ttcccgatcc gcagtcgata ttgtcctgca
     8881 gccacgcaat cagaatatcc agcgctgttt ccgtggttaa tgatttcatg ttgttcctgg
     8941 ttaccagtcg aaagtgggta aacccggcag gcattcagcg ctggcatcca gatgaatgag
     9001 actgacgcca taacgccgga tgagtgtgac gaccagacga cggaacgttt ttgacagccc
     9061 caggcgttta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca gatatccgct
     9121 acctgtaaaa tgaatccatt ctgattcacc gaagtcactg gtctggtgtg acagcgagta
     9181 caaccaggcg ttgtccgttt ccgtgatatg tgcggtactg cagcgtatgc cggtaagggt
     9241 agcaaatggt ggcgtgcatt ctgctgaggt ctggtcagat tcatcaacaa cacgaaggga
     9301 gtagccgtta tcgcagacca tgttaatcag ttcagcgagc tcaataccat cgacgtcaac
     9361 gacaatgcgc cctatgttca gtgcctgtac gttaacgctg tcggcttcca gtgtcagggc
     9421 cagtttcatc atttcgcctc cggatactta cccagggtaa tgttatttac cgttctgtaa
     9481 ttgtcgcggg tcatcaggcc ggtcgcccgg cgagcccgga ggatatcaat actgtttatt
     9541 aactgagagc ggggacaggt gctgaatccc ggctggtcgg tacgcaccag cgcgtatttt
     9601 tccacgagaa aattaactgc atcacacagt gaaatgcctg cctcaatatg ctgctcaatc
     9661 acacgttcat cgacaaacgg tgtgtcattc agtgtgaggc cataatgctg gtccagcagt
     9721 cggatgagca gtgtctgcca gatggtgacg ggagacgggc agcgagatac ctcccgtaca
     9781 tgtgtgtcgg ataatgtttt catcttaagt tctctgtaac tggtagtgct aaaggtgaga
     9841 gcttcggggt tatgaggtgg ttgcgggtgc tggtgtcgga taaacagcca gataaacgta
     9901 accacaggag ccgagggtgt cggcttcgca ggtcagccct tttgcataca gcgtgacggt
     9961 atgctgatat cggggattca gttcaccgct ggtgagcatg agttccagtt gtttcatcag
    10021 cagcggaaag gcctggtcca gatggtatgc atctgcgtcg ctgaagcggc ctctgatacc
    10081 ggcgcggtcg gcaaggtaat acaggcggtt accctcttgc accagacgag cgccaaaaca
    10141 gggcctcact gtgcagggaa gcccccacca ggggcggtcg tgattgtcat cgggatgagt
    10201 tatcccggag agtttgtctg acacgataaa atcctctctg aaaagtggtt aagaatggtt
    10261 taacgatgac aggtcagccg gcgcgctgac cggtttcggt gaagatgtat gcattgatga
    10321 gaatggcctc atccacagcg gcgtctgagg tcatctggtc gtactcgttt cccgggtgac
    10381 ggaacatcag ttcagggtga cactcatcag ccggacatac gggccaaaac tgtccttacg
    10441 gcgttctgca aacacggcaa gaacgccggg aatatcctgc acttcacgac cagtatacgc
    10501 ttcagcactg ccgtgccagc gatacttacc ggtacagaac ggaaaaagac gggatgcagg
    10561 atgctgttgg tgaatacgca tggcttcacc acgggtgatg attctcatga tgtgatacct
    10621 ctgaagacag aagataaaag tgaaaacagg tgtgatgtgg ttgtgacggg ttaaagcaga
    10681 ccatgttcgg caaaggagaa aacctggttg ccaccgacta tcagatggtc cggcacccgg
    10741 atatccacca ggcccagtgc ctgtaccaga cgttcggtga taagccggtc tgccttactg
    10801 ggtgtgactt caccggacgg gtgattgtgc gccagcacca cggcagcggc attgtggtac
    10861 agggcgcgtt taatcacttc ccggggatga acttccgtac ggttgatggt gccggtgaag
    10921 agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg gaactcttcc
    10981 cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcacgggt ggaggtgaag
    11041 gccacgccgg gttcatgaag atggcggtcc agggtttgca gggcccgcag aatgagactg
    11101 cgctcgccgg gcgtcatgtt tccgggcaga aaggaaagct gctgcattgt ccctgcctcc
    11161 attcagtcga tgatgcgcat aatggcgctg cattccggat gctgcagggc gtaatcccgc
    11221 agccggtaat aatggaccgt catggcataa cactccgtac gacaggcatg atgactgtac
    11281 gtcatcagac aggcagcaat gccggcggct tccgggctca tttcagcgcg gttaccgttc
    11341 atggcattga acagtaccca tgtttcgtca tcatcgtcat ccggttcggg tgccataaat
    11401 gccccgccgt tgttcagggt gtacagattc cagataccac cgcagtagtc ttcacacaga
    11461 cggtccatcc agccgaagac acggggttcc agggtgaccc actgtggaat gaggccaaaa
    11521 tgctgcggcc agaagctgat gcgctgttca tcagggacgg gagtggcaac cagttgttgc
    11581 aggccagtct ccggtgcaca ggccgaagcg gtggtgttct gagacaatgt tttcatgtat
    11641 gaaatccttg taatgtgaat aaatgagagg gagtaaaggt cgggagctgg caacatcacg
    11701 ttcatcactg accgtgatga cccggtaata tcagggatga atgatattcg gctgaaattc
    11761 cggacagccg tacgcgtaac cgttccggtc acagacggat ggccagatgc cattgcctgt
    11821 atactcagtc tgcacagact gatttctccg ggacggtaaa gcagacgggt acaccaggcg
    11881 cacaggtcat catcagaaat ggaacgacac cccggcagcg cctccggggc gtcgggtgat
    11941 tctggtgcct gaatcacgcc ttcagggaca tgctgcctca gcgcaggctc tccagcagcg
    12001 tttctgccat tacccacaat gcgcggttga gcttaatatc ggtgtcgatg ttgtgaatgg
    12061 cacgggtgtg gatacgtttt ccttttgcac tgcggccgga aatcccgcct ttcagcatat
    12121 tctcctggat ggtctgatat gcgctccaca ggtctttacc gtaatcctcc ctgcgtcgtg
    12181 gtgtcagaat gtcagcggtg gtgactggct gatgttcgtc accataacgg taagtcagtg
    12241 ccgcctgtgc cagcgcctgg cgtgccggtg gcggcaggac cagcgactgc atggcatcac
    12301 gcttctcctc tatccggtca aacacgccca ccacctcgta agccccttca ataactttct
    12361 ccaccacatt tccccggtgt ggaacacgca attctcccag agactgaccg cagaccagtc
    12421 cattggtaca aatggcacga aaatatccgg gtaacatctg gtagctggat gaaccgtcat
    12481 gagagttgag cagaataatt tcagggacat gctgaccggt tatctgtccg gcccgccgca
    12541 gacgcagcat gtgttttgtg tattcccggc gacccgggtc acgcacacgt gtctggcagg
    12601 cgaagaatgg ctgaaagcct tcccgctgca ggctttccag tacggtgatg gtgggagtgt
    12661 acgcatagcg tttactgcgg gaagtatgcc ggtcttcacc gaaaatactg ggaacatagt
    12721 gcatcagttc ttcgtgtgtc agcggacggt cacggcgtat ctggtttgca gcataaccaa
    12781 aacgactggc tagtcgcata atttgctcct tatcggtggt taagatttac tggtgtaata
    12841 aatgaaaaag ccacgtctcc cggagaagac gcggcctgat ggacgaaatt aatgaggttt
    12901 attgtctgag aagaccgtca ctggcgaacc ttccggcgtc agtctctgcg ttgcggataa
    12961 cccgacggaa atgagttccc tggtcatcgt cgataaacac attgcggtaa gctgttgtga
    13021 tgacaacagc ctgctcgcgt gagaaaggga cttcgggcaa taaacgtgac atacaaccag
    13081 acctgtattt ggttccctga aaatataagt ccctgtcatc atggcagagc cgtatttact
    13141 tttgatatca ataaattaat aacgcgaaga tgaaccgcat aacttacgcc tttaatttgt
    13201 tttgtttacg tagtccctga ccaggtacgc gaattcgtga acgccagttt caccaaacca
    13261 aatcaggtaa ccagggtcga tgttgtaata gtaaccaacg actttatgcg tttttgggtt
    13321 atagagtgtt acgatatagt cccagccgca ggcgtgggct ttacaaccgc caccttctac
    13381 ccaggtcgtt ccgttaatgt ttacaggata cgcaccacca tcaggaccat ccatatagcc
    13441 gtcttcccca aacattccgt ttaacccaac agggtttaat gctttgtcga tagcagctct
    13501 gtaggtagcg tcaccttcgt ataactcgta cgttgttgga catgcaccag gctggtcagc
    13561 ccgggctttt gagtcgcatt taagtgtctg atttacagtg gcggcgtttg tgtcaggact
    13621 t
//
LOCUS       DAJTUN010000107        13413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTUN010000107 DAJTUN010000000
VERSION     DAJTUN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.107"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(103..825)
                     /locus_tag="OO470_003041"
     CDS             complement(103..825)
                     /locus_tag="OO470_003041"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6252221.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(827..1998)
                     /locus_tag="OO470_003042"
                     /pseudo
     CDS             complement(827..1998)
                     /locus_tag="OO470_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2025..3641)
                     /gene="bglH"
                     /locus_tag="OO470_003043"
     CDS             complement(2025..3641)
                     /gene="bglH"
                     /locus_tag="OO470_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6252222.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(3727..5121)
                     /gene="bglB"
                     /locus_tag="OO470_003044"
     CDS             complement(3727..5121)
                     /gene="bglB"
                     /locus_tag="OO470_003044"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6252223.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5140..7017)
                     /gene="bglF"
                     /locus_tag="OO470_003045"
     CDS             complement(5140..7017)
                     /gene="bglF"
                     /locus_tag="OO470_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6252224.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7151..7972)
                     /gene="bglG"
                     /locus_tag="OO470_003046"
     CDS             complement(7151..7972)
                     /gene="bglG"
                     /locus_tag="OO470_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6252225.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8259..8984)
                     /gene="phoU"
                     /locus_tag="OO470_003047"
     CDS             complement(8259..8984)
                     /gene="phoU"
                     /locus_tag="OO470_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6252226.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(8999..9772)
                     /gene="pstB"
                     /locus_tag="OO470_003048"
     CDS             complement(8999..9772)
                     /gene="pstB"
                     /locus_tag="OO470_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6252227.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(9864..10754)
                     /gene="pstA"
                     /locus_tag="OO470_003049"
     CDS             complement(9864..10754)
                     /gene="pstA"
                     /locus_tag="OO470_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6252228.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(10754..11713)
                     /gene="pstC"
                     /locus_tag="OO470_003050"
     CDS             complement(10754..11713)
                     /gene="pstC"
                     /locus_tag="OO470_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6252229.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(11800..12840)
                     /gene="pstS"
                     /locus_tag="OO470_003051"
     CDS             complement(11800..12840)
                     /gene="pstS"
                     /locus_tag="OO470_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6252230.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13088..>13413)
                     /locus_tag="OO470_003052"
     CDS             complement(13088..>13413)
                     /locus_tag="OO470_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6252231.1"
                     /translation="TVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKI
                     YDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATIT
                     VEIVR"
BASE COUNT         3424 a         3502 c         3068 g         3419 t
ORIGIN      
        1 ccactgcggc aagctgagac tgtggggtga ttatttcgta gttatttaaa taatgagaac
       61 aggccggagc gtaattcata catccggcct tatttcttca gcttaaccga acgctaattc
      121 tgcggcagcg gctttatcag cgattaccat cagcgaagga tgcagctgca acacggaagc
      181 tggaacatct tcggtaaccg gaccttgcag cacatttttc agtgcctgcg ctttacctgc
      241 gccactgaca attatcagca agtttttcgc cgccatgatg cttttcggcc ccatcgtcac
      301 gtagctgtca ggcactaacg aaaagtcgcc gcccaactca ccatgcgcca caatatcaac
      361 catctctccc tggatcggga actccacggt ttgctcatgg aaatgggtcg tattcggcaa
      421 attgccacaa aaatgaccat ctgcacctaa tcccagcacc accaaatcca ggccgccttc
      481 acgcgccagt ttctgatcat gctcgcggta gttatcaata gtgagcttct ggatattctc
      541 ttctttgatc cccgcagggg tgaaaaacag attacgcaga ttggtaatcg ttacgccttc
      601 tccctctttg ccgcgaaagg gaatttcatc gaaattatag aaatagcagt tatcgtacca
      661 aggcttacct ttaaccaggg tggtgaggta ttcatacatg cctttgggcg tgctaccggc
      721 ggtaattgcc aggttaacac gacgcgtctt cgacatataa ccaagcagat ggtgtgccgc
      781 gacacggctc atttcctggt aatcttcggt aatgattaat ttcatcttaa tgccttattt
      841 taaatttctg tacgaaggct gcataggcag gccgccagac atccatttca tggttcaggc
      901 cgggatattc ctggtaatca aacttaattt ttttctgctc aagctcagtt ttcagcccgg
      961 cgatatcctt gccggttacg acatctttat caccaacgac cacagtaaaa ttacgtagtt
     1021 gctggttaat agctgccgga tcgttcagcc gggccgcgac accttcatcc ggtacggttg
     1081 tcgtggtaac accactgaat gtggccagcc aaagctttcc agatgattca ttccggaaac
     1141 cagcgcctgg tacccgcctt gtgaaagccc tgccagcgcg cgaccatcgg catctttacg
     1201 gacactaaaa cgcttgctaa tcagcgggat aatatcgttc atcagttcgc gatctgccgc
     1261 tttagcattc agcggataaa agactttacg tctttcctga ggcacgaaat cttcgggaat
     1321 aatgcccttc gcatcggttt cggtatccgg gatcaccacc agcatcggtt taattttccc
     1381 ttcagccagc aggttatcca tgatttgcgg gatacgcccc tgatcgatag cggaacgtcc
     1441 ggtatcacca aaaccgtgat agaaatagag cactggcaaa ggctcgccca tgccggtgta
     1501 tcctggcggg gtccagacat acatctgacg ttcagattgc aatgcgttgg agtggtaagt
     1561 tatggcgatc aaatcaccat gcgcaacaga acgcgtatcc agataactgc ctggcaccag
     1621 aatcatactg gagttaacct ggcgctgagg cttagtcatt gcggtgcctg tatcaatgct
     1681 gcgtacacca tcaacattga aaaaatactc gtacaggttg cctttcagga taggtgtgcg
     1741 ccacgaccag actcctgctt cgtctttggt catcgggtga atattgtccg gaaccggaac
     1801 acccaccact acagagacat tttttgcccc aggggcaaag tagcgaaagg tcacgctgtt
     1861 atcggcattg acctgagtca catactgttt caccgggata gtgggtgccg ggctggctgg
     1921 catatccgct gctatggccc actgagcaga aataccgctg gcaatagcca gcgttaaagc
     1981 agcaattttt atattcatcg taatattcct ttcattaagt tccattacca ccagatttca
     2041 gcctgggcac cgacagcaaa ctgggcgtct ttattgtctt cgaaggtgaa gccatccagt
     2101 tcgttttcca gggctttgat atacgtggcg tagaaacgaa tttccagacg cgaggtcaac
     2161 atactggtat tgactttaaa ggtatggaag agcgtggttt tataaccaga ctcattaaat
     2221 ttattactat tcgcatcttt gttttgctgg gtgaaatagc ccagttcaac acctgtctgg
     2281 ttatattggt cccaaatata ggccgggcga acaaccgcac gaatagattc gaaatcagag
     2341 tgtgcgcctg tttcgtagct ataaatattg ttaccgaagg agtaaacaat cgcgttagcc
     2401 acaataaaat gatcgccgat ataggcttcg ccctgcgagg tcagacgaac tgctgttccg
     2461 ccggtgtgat caccataata acgaccatta aaggtggtaa atggacttgc gccagcataa
     2521 cggccaaagt tcctggcgat agagttattc gcaaccagga aggagaattc gttgaagcca
     2581 cctttatcaa atttctgcgt taaagatgtg ccgaacatcc aggtatcttt ccagtcataa
     2641 tacccgttat tatcctgatt atctttttcc gatgcgcttt cgtttgccgt gacataacga
     2701 ccactcacca ttaaggtcgc tttatcccat aacgggatat ctttatagcg taaatcaata
     2761 gtatgggtat taatctgctg tttgttttgc aggctgcgat cgtaatcatc gacatcttcg
     2821 cgaaccagcg cgatatcaat tttacccgga ccgactttcc agttttccag acctacaccc
     2881 gctgcagcat cagtacgctg cgttttccag tccagcatct ggatttcaat tttcggcgca
     2941 ccgtgtttac ccacccagaa atcagcctct ggcgcaaagg gcaggaaacc tttggtggta
     3001 acgtacatat cggagaactg cataaagttc tcgccaccgg cattatcgcc aaaccagccg
     3061 gtagagtact gctgaccaac gttaccatct atcatcacaa cggcatcaac ccgtttgccg
     3121 ttttcgttgt agacacgttg ttttaactgc aaatcaaacc agccggagta ttcgttacca
     3181 aagcggccca gagaaccaat cgcccatgat ttaggtgaac catgagaggc ggtcccccaa
     3241 ccagaacggt aatagccgtt gtaactaaag ccaatttcat ctttcacaaa cttactgaaa
     3301 tctttcagag tcatggaaga ataaatcgag cctgtctcac tggcattttt ctcttcattt
     3361 ttgaccagta cagcatcagg ttttgcggca ctactggtcg ggaacggatt ggcggcatac
     3421 ccttgatcat tcgtacttac gctacgattc accgtcgctg gcgtatattt tttcttctct
     3481 tcatctttat actttttgag ttcgctttgc gtttctctta acgctttttc taataactca
     3541 aggcgttgtt ccaccgttaa tgattcagcc aatgattgtg cagaaaaggc taacggtgcc
     3601 attaataaga tggcagaggt aataagattt cgtctaaaca taatatccct ttatggtgca
     3661 aagaaagaat taacgtatcg catccagact gttctgaatg cgacgataat taaggtgctt
     3721 tattggttat ttttttaatg acagcccccg cgttttgatc acttctgcat accatccgaa
     3781 gcttttctta cgcgttcttg tgaggcttcc ttcgccatta tcatcacgat ccacataaat
     3841 aaagccgtag cgcttagaca tttgtgaatg agatgcactg actaaatcaa ttggccccca
     3901 actggtgtac cccataatat ccacaccatc ggcaatcgct tcatttacct gtaccaggtg
     3961 atcgtttaaa taggcaattc gataatcgtc ctgtatcgaa ccatccgctt caacgctgtc
     4021 ttttacgcct aatccattct cgacaataaa taacggtttt tgataacgat cccaaagcgt
     4081 atttaacaga acccgtaatc caaccggatc aatttgccac ccccactctg aacttttcag
     4141 atgcggattg gggatcatat tcagtatgtt gccctgcgca tttttattaa tgctttcgtc
     4201 gtgggaaaca caaccagtca tgtaataact aaaagagatg aaatctacag tatgttttaa
     4261 atcttctgcg tcactttcag tcatctcaat ggtgatattg tggtcgcgga agaaacgctg
     4321 catatagccg ggatattggc cacgcgcctg aacatcacca aagaacatcc agcgccggtt
     4381 ctcttccatg gcctgcaaca tatcctgtgg ctggcaggtg agggggtaaa ccagcccacc
     4441 gagaagcata ttgccgattt tcgcttcggg gatcaggcta tgataggctt taactgcccg
     4501 cgcactggca accagttgat gatggatcgc ctgataaact tccgcctcgc cactctcttc
     4561 tgccagcccc acgcccgtga atggcgcgtg taacgacata ttgatttcat taaacgtcag
     4621 ccataacgct actttatgtt ggtagcgggt aaagaccgtg cgggcgtaat gctcgaagtg
     4681 atcgatgacc gcccgattag cccaaccgcc gtagtttttc accagcccat atagcatttc
     4741 gtaatgggat aacgttacca gcggcttgat ccccgcctgc gccatttcat caaacagccg
     4801 atcgtaaaac gctaaccccg cttcattcgg ttcggcttcg tcgccctgag ggaaaattcg
     4861 cgcccaggca atggaaatac gcagacaggt gaagcccatc tcggcaaata acgcgatatc
     4921 ttccgggtaa cggtaataaa aatcgatggc gacatctttg atattctctt tccccaggat
     4981 gcgcggttcc atttttccca ttacgccatg aggctgtaaa tctgaggtcg tgatcccttt
     5041 gccatcttcc agccaggcac cttccacctg attggcagct gttgcaccac cccaaagaaa
     5101 tgtttctgga aatgctttca taattaactc cttttatcgt tagcgaatga tggataacag
     5161 cggttcacct gcgcttatct gcgccgtgcc gtggggtaat acgtctgtaa aatcatcgct
     5221 attactgatt aataccggcg tcgtcagatc aaatccggcc tcgcgaatag cagggatatc
     5281 aaaagaaatc agccgatcgc ctgtattgac cttgtcaccc acgttgacgt gagcggaaaa
     5341 gaatttgccg tccagtttta cggtgtcgat accgacatga atcaggatct ccacaccatc
     5401 atctgactca atgccaatgg cgtgtaatgt ggcgaacaac gaagcaattc gacccgcaac
     5461 cggagaacgc acttcaccaa ccgagggcag aatggcgata cctttaccca acaggccact
     5521 ggcaaacgtg gtatcagcga cgtgaatgag cggcacaatc tctcccgtca tcggtgaaca
     5581 gataccgccc tgctcaggtg gtgtaataac ctctggtgtt ttctcttgcg gggcaccctg
     5641 cgctggctga cgtttactgg tgatgaaatg aagcatcaac gtaccaacaa atgcgcaacc
     5701 tatggcaatg acaccgccaa taacgctggc ccagacggtg aaatcaatcc ccgttgacgg
     5761 gatggtctgc atgaaggtga aaatacttgg caaaccaaag gagtaaactt tcgtttgcgc
     5821 gtagccaatg atggtggccc ccaatgctcc actgatacag gcgataacaa aggggtactt
     5881 acgcggcagg ttgacgccat ataccgctgg ttcggtgata ccaaacagac ccgtcaacgc
     5941 cgctgatccc gccaccactt ttttctgcgc atcgcgttcg cagaggaaga cgccgagcgc
     6001 cgccccgacc tgcgccataa tggcgggcat taacagcggg atcatggtgt cgtagcccag
     6061 cacggtgaag ttattgatac acagcggcac caggcccctg tgcagtccga acatgacgaa
     6121 gatttgccag aagccgccca ttaccgcgcc cgcaaatgca ggaaccgcct gataaagcca
     6181 gagataaccg gcggcaatca tttcgcttat ccaggttgat agcggcccca ccagcagaaa
     6241 ggtgacgggt gtgataacca tcagacatag caatggtgtg aagaaatttt tgattgccga
     6301 cggtaaccac gcattaagtc ggcgttccag aatgctgcac aaccaggcag aaaaaataat
     6361 gggaataacc gatgacgagt aattcaacaa tgtgaccgga atacccagga aatccagccc
     6421 cggcgcatcc gctttttgcc cgttctcgaa ggcagtcaga attaatggat gcactaacgc
     6481 tccaccaatc accatggcag taaatggatt accgccgaag cgtttgcccg cggtgtatcc
     6541 caggattatc gggaagaacc caaacaaggc atcactggcg ctgaataaaa ttaaataagt
     6601 accactttgt tcggtcgtcc actgaaaagt gagcgccaga gccagcatac ctttcaggat
     6661 cccggttgcc gccatcagac cgatcagagg cgtaaaaata cctgaaataa cataaacaaa
     6721 gcggtttagc agattacctt tctcatcatt ttccggtgcc tgttgcgctt tttcgccaag
     6781 gcctgccacg ctgttaaccg ccaggaagac atcggccaca tggttaccta tgaccacctg
     6841 aaactgacca ccgctttcca ccaccataat aataccgggg gtctttttca gtacctcagc
     6901 ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc gttgcgcaat gcatcagact
     6961 cacaatgtta tctgcgcccc cgactcctgc gactattttt ctggctaact ccgtcataac
     7021 ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaaac ctgaaaaaaa cggcctgacg
     7081 tgaatcaagc aatttttttc aggttttgcc cgcttagtgc ggtaacaatc ctttactcag
     7141 taataatatt tcagtgttct ttgcgcacgc gctctatatt tatggctaaa aacataatct
     7201 ctgcgggtga aattttacgt tgatactgca aaccaataaa aatggcgatc cgttccgcac
     7261 attgccatgc ttgcgggtaa ttttgtttta ctgcttgttg taatgattca tcactatcgt
     7321 taattgaagc atgttcaaga atacgccaag ataaaaactt cagatgtgta accagtcgct
     7381 gataactcaa gctttcttcc tggtaattaa ggctgaactg aaattttatt aattgcagca
     7441 tttcgcgcat taactgcgtg acacctgcaa catcctccat atttccgctc atttgggcac
     7501 tgaccagatg catggcaata aagcccactt catctttcgg taactgcacg cccaaccgtt
     7561 tatcaataat ggttaacgct tcttccccta gctggaactc tttcgggtaa agccgctgga
     7621 tatcccacag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
     7681 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataagac
     7741 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
     7801 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
     7861 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
     7921 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
     7981 ccttttttat aaacaaaaaa aaaccgactt caccagtatt ctctggttat gtcaggtttt
     8041 gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg
     8101 ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga taaaaatatg
     8161 accatgctcg cagttattaa ctttatgtaa ttttaggaat ttataaagtt atatataaca
     8221 aatcccaata attaagttat tgggatttgt ctggtgaatt atttgtcgct atcttttccc
     8281 gccagcagtt tatccagctc atcaccgcct acatgacgga aatcctgacc cttcacgtag
     8341 tagaagataa actcacaaat attctggcag cggtcgccga tacgttcgat tgaacgcgca
     8401 cagaacagcg cggtaagtac gctcggaata gtgcgcgaat cttccatcat gtaggtcatc
     8461 agttgacgaa caataccttc gtattcctga tcgacttttt tatcttcacg ataaatacgt
     8521 accgcttcgt caatgtccat ccgcgcgaac gcgtccagca cgtcgtgcag catctggatg
     8581 gtatgacggc ccagcgactc cagacttacc agcaacggct gatgctgctg ggagaatttc
     8641 tccagcgcag tacggcagat tttgtccgcc acgtcgccaa tacgctccag ctcggcaatg
     8701 gttttactga tcaccataac cagtcgcagg tcgctcgccg tcggctgacg tttggcgata
     8761 atgcgcacgc aggcttcatc gatcgccact tccatcatgt tgacgttctt gtcgccttcg
     8821 atcacgcgct tcgccagatc gctgtcctgg ttatgcatcg cggtgattgc atcagaaagc
     8881 tgctgctcca ccatgccgcc catggtcatc acctgcgtgc ggatactttc cagttcggcg
     8941 ttgaactggc cggaaatatg tttattaaga ttgagactgt ccataacgca ctcctgaatc
     9001 aaccgtaacg accggtgatg tagtcttctg tttgtttctt cgctggcttg gtgaacagat
     9061 cgtccgtgtt gctgaactca atcaattcgc ccaggtacat aaatgccgtg tggtcggaac
     9121 aacgcgcagc ctgctgcatg ttgtgggtga cgatcaccac ggtgtaatcc tgcttcagtt
     9181 cggtgatcag ctcttcaata cgcccggtag agatggggtc gagcgccgaa cacggttcgt
     9241 cgagcagcag cacttccggg cgaatggcga taccacgcgc aatacacaga cgctgttgct
     9301 gaccaccaga gagagagtaa ccgctctggt gcaatttatc tttggtttcg ttccacaatg
     9361 cggctttggt caatgcccac tgcacgcgct cgtccatgtc ggcacaggag agcttctcaa
     9421 acagacgaac gccaaaggcg atgttgtcgt agatggacat cgggaacggc gtcggtttct
     9481 ggaacaccat gcccactttc gcacgcagca gggcgatatc ctgagagttg gtcaggatgt
     9541 tgtcgccatc aagcagaatt tcaccttccg cacgctgctc cgggtacagt tcaaacattt
     9601 tgttgaaggt acgcagcagc gtcgatttac cgcagccgga cggcccgata aacgccgtaa
     9661 cctggttttt ggcgatatcc aggttgatgt ttttcagggc atggaatttg ccgtagtaga
     9721 agttcaaatt acgaacctga attttactcg gggcagtttc aaccatactc attgcaatct
     9781 tttcctcatc gtacctgatg cttttcgcta atcaggctta ccgtagggcc gggtaaggcg
     9841 ttcacgccgc atccggcaaa gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgcc
     9901 agaatgttca gcagcaggac gcacagggta atgatcaata ccccggccca ggccagttgt
     9961 tgccattccg caaacgggct catcgcaaac ttaaagatcg tcaccggcag gttggcgatc
    10021 ggctgcatca tgtccgtgct ccagaactgg ttggagagcg cggtaaacag cagcggcgcg
    10081 gtttcaccgg caatacgggc aatcgccagc aggataccgg tcataatccc ggacaccgac
    10141 gctttcagcg taatcgcgga gatcatcttc cacttcggtg tacccagcgc ataagccgct
    10201 tcacgcaggc tgtccggcac cagtttcagc atgttctcag tggtgcggat aacgatcggc
    10261 acctgcaaca gcgccagcgc aattacgccc gcccagccgg agaagtgctc catctgtgcc
    10321 accacgatgg tgtagacaaa cagaccaacc acaatcgacg gcgcggagag cagaatgtcg
    10381 ttaatgaagc gaatcacttc tgccagccag gatttacgac catattccgc cagataaatc
    10441 cccgccataa tgcccagcgg cgtaccgaat accgtggccc acaaaattaa cagcccgcta
    10501 cccgccaggg cgttcgccag accaccacct tccgtattgg gcggcggcgt catttcagtg
    10561 aacagcgcca gcgacatacc gtcgataccg cgagtgatgg tggacattaa aatccagatc
    10621 agccagaaca ggccgaaggc catcgtcgcc atcgagagcg tcagcgcaat acggtttttg
    10681 aggcggcgac gcgcctgcat tttgcggcga gattcagcca gcgccgcagt ggtttgcatt
    10741 tcaaccatag ccattagcgt gccccctcat tcttagccag gcgcataatc ataaacttcg
    10801 atgcggcgag gacgatgaag gtaatcacaa acaggatcag gcccagttcc atcagtgcgg
    10861 caacgtgcag accggattcc gcttccgcaa attcgttcgc cagcgcagag gtgatactat
    10921 tgcccggcat gtacagcgag gcgctgtcga gctggtaggt gttaccgatg ataaaggtca
    10981 ccgccatggt ttcaccgagc gcgcggccca gccccagcat gatgccgccg ataacaccat
    11041 ttttggtgaa cggaagaacg atacgccaga taacttccca ggtggtgcag ccaataccgt
    11101 aggccgactc tttcatcatc accggggttt gttcgaacac atcacgcatt accgccgcaa
    11161 tgtacggaat aatcatgatg gcgaggatca cgcctgccgc gaggataccg ataccaaatg
    11221 cggggccaga gaacagcgcg ccaacaatcg ggatattcga catgatattg ccgaccggct
    11281 cctgaaagta aacggcgaac agcggcgcaa agataaacag gccccacatg ccgtaaacga
    11341 tacttggaat ggctgccagc agctcaatgg cgatacccag cgggcgtttc agccagccag
    11401 gcgcaagctc agtcaggaac agggcgatac cgaaactcac cgggacggcg atcagcagcg
    11461 cgataaacga agtcaccaac gtaccgtaga tcggcaccag cgccccgtag atatcgttcg
    11521 gtgcatccca ctctttggtc cataggaaag ccagaccaaa tttctgaatg ctcggccagg
    11581 aggagatgat cagagagaca ataatgccac ccaacatcaa tagcacaatc agcgccgcca
    11641 gttttaccag cacgctgaaa attatgtcgc cctttttacc cggtgggtta aaagcgggct
    11701 tggttgcagc cataagttac tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc
    11761 ggatgcggcg taaaacaccg tacccggcct ggagttttat tagtacagcg gcttaccgct
    11821 actgtcttta atattggtct tccacgcagc gcgaacctgt tcaactacac tatccggcag
    11881 gctggcgtaa tccaggtcgt tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa
    11941 tttcagcacc tctgtgcctt gttctggttt cttctgatct ttgtggatca gaatgaacgt
    12001 ggtagaggta ataggccatg catcttcgcc tttctggttg gtcagatcct gagcgaaggt
    12061 tttgctccag tctgcacctt ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg
    12121 tttaccatca gcggagatca gtttggtgta cgccaggttg ttctgcttcg cgtaagcata
    12181 ttcaacataa ccaattgcac ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc
    12241 tttaccgccc agaccgatcg gccattttac ggtagagcca gtaccaacgt tgtttttcca
    12301 ctcttcgttc actttcgcca ggtagctggt gaagacgaag gaagtcccgg agccatctgc
    12361 gcggcgtact acagcaatgt tttgtgaagg cagtttcaga cccggattca gtttggcgat
    12421 ggcttcatca tcccacttct tgattttgcc caggtagatg tcgccgaggg ttttaccatc
    12481 cagcaccagt tcgccagact tcagccctgg aatgttaacc gccagcacca cgccgccaat
    12541 cacggtcggg aactggaaca gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc
    12601 agaggcacca aaatcaacgg tattagcgat aatctgtttt acgccaccgg aagaaccgat
    12661 accctggtag ttaactttat taccggtttc tttctggtaa gtgtcagccc atttggcata
    12721 caccggcgca gggaaggttg cacctgcacc tgtcaggctt gcttctgcaa acacagagaa
    12781 agcactcatc gataaggtcg cggcgacaac agttgcgacg gtggtacgca taactttcat
    12841 aatgtctcct gggaggattc ataaagcatt gtttgttggc tacgagaagc aaaataggac
    12901 aaacaggtga cagttatata taaggaatat gacattttta tgacagaaag gaattaccaa
    12961 ataacttact gattttaaat gtgaagatga aaatcccaca tgccatcttt ttgtcatgat
    13021 gcatttaaaa gtgataaaga aatgggtgag atgacaacga tgtgatgaac aatcgttgtc
    13081 agacctatta gcgcacaatc tcaacagtta tcgtcgctgt tgcagtaaat tgccccgggg
    13141 ctgggcgagc tccagtaagg ctcgcaggag cagctgaaaa agttacacta cctgattttt
    13201 caccatacac tgttgattta tccatgtctg ttggcaactc accaccgttc actttgattt
    13261 ccttattatt tttgtcgtaa atttttatcc ccacgttaga gtttgaagtt acgaccattg
    13321 aattgtccgt attgggttcg gtagcataaa aagacgtact gatatcatcg gtattcagta
    13381 tccccaggtt ttcacactca atagtgactg ttt
//
LOCUS       DAJTUN010000108        13283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTUN010000108 DAJTUN010000000
VERSION     DAJTUN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.108"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1240)
                     /locus_tag="OO470_003053"
     CDS             complement(<1..1240)
                     /locus_tag="OO470_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6252232.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSL"
     gene            complement(1233..2228)
                     /gene="hlyD"
                     /locus_tag="OO470_003054"
     CDS             complement(1233..2228)
                     /gene="hlyD"
                     /locus_tag="OO470_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6252233.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(2231..2902)
                     /gene="cecR"
                     /locus_tag="OO470_003055"
     CDS             complement(2231..2902)
                     /gene="cecR"
                     /locus_tag="OO470_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6252234.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            3131..4318
                     /gene="rhlE"
                     /locus_tag="OO470_003056"
                     /pseudo
     CDS             3131..4318
                     /gene="rhlE"
                     /locus_tag="OO470_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(4525..5007)
                     /gene="ybiA"
                     /locus_tag="OO470_003057"
     CDS             complement(4525..5007)
                     /gene="ybiA"
                     /locus_tag="OO470_003057"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6252235.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            5127..7277
                     /gene="dinG"
                     /locus_tag="OO470_003058"
     CDS             5127..7277
                     /gene="dinG"
                     /locus_tag="OO470_003058"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6252236.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            7305..8267
                     /gene="ybiB"
                     /locus_tag="OO470_003059"
     CDS             7305..8267
                     /gene="ybiB"
                     /locus_tag="OO470_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6252237.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            8408..9493
                     /gene="hcxB"
                     /locus_tag="OO470_003060"
     CDS             8408..9493
                     /gene="hcxB"
                     /locus_tag="OO470_003060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6252238.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(9722..9982)
                     /locus_tag="OO470_003061"
     CDS             complement(9722..9982)
                     /locus_tag="OO470_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6252239.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(10247..10513)
                     /gene="ybiI"
                     /locus_tag="OO470_003062"
     CDS             complement(10247..10513)
                     /gene="ybiI"
                     /locus_tag="OO470_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6252240.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(10587..11264)
                     /gene="ybiX"
                     /locus_tag="OO470_003063"
     CDS             complement(10587..11264)
                     /gene="ybiX"
                     /locus_tag="OO470_003063"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6252241.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(11306..13283)
                     /locus_tag="OO470_003064"
                     /pseudo
     CDS             complement(11306..13283)
                     /locus_tag="OO470_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         3013 a         3483 c         3615 g         3172 t
ORIGIN      
        1 agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg gaactctctt
       61 tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa ccgcacatca
      121 tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa atctccccac
      181 gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc ttggtcagtt
      241 ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat attgcaccca
      301 gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct tcaaaacgcg
      361 gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata tcgtctggtg
      421 tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgcca tcgctgacct
      481 gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga ctggtcatca
      541 gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga tacagcaact
      601 cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga tacgaggtac
      661 tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac agttcgcgcc
      721 gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc ggttcgccca
      781 ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg cccgccaggc
      841 gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg
      901 cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc accgtgagat
      961 cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc gcgccgtcgt
     1021 ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc agtaatcccg
     1081 ccaacatccg catcagtgtg gttttacctg caccgtccgg ccccaccaac cccgtcacat
     1141 aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg cccggaaagc
     1201 gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc gtcaccgaat
     1261 tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc
     1321 aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg ggtaaattca
     1381 gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg accatcggta
     1441 taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc aacataagcg
     1501 cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc attgagaacc
     1561 gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat caacgttgag
     1621 tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc
     1681 gcctgagcga tgtcctgttc acggttaccg gaacggtact gacgcaattt atcctgtgct
     1741 gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag gtcatttgcc
     1801 gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg cgcatagtca
     1861 taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc atcgcgatac
     1921 ccggcaagca tcagattata ctgcgcctgt gccaccgaga cgcccgcttt cgcctgcatc
     1981 agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc tttgatagca
     2041 tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact aagattcacc
     2101 gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct ttgataccac
     2161 cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat cacgacaggt
     2221 tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg atatgacagg
     2281 tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag ccggtacgta
     2341 acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac gcatgggtat
     2401 gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc aggcgtgtca
     2461 ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct gccgtgggag
     2521 agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg gtgagcagct
     2581 taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga tcaggctgtg
     2641 gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg ccaataaaat
     2701 cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga
     2761 tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg ttcattccat
     2821 attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt tcacccttga
     2881 ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt gattaagatt
     2941 atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc agtcacaggg
     3001 ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga cattgtggtt
     3061 tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt cggggcggtt
     3121 cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc gcgccgttgc
     3181 cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg cggtgctgga
     3241 aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg gctttacgct
     3301 gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg
     3361 tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa acgtccgtga
     3421 ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca gcattaaccc
     3481 acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg gacgtttgct
     3541 ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga
     3601 agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt taacaaaact
     3661 gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata ttaaagccct
     3721 ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca ataccgcgtc
     3781 tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg aattgctgtc
     3841 gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta ccaaacacgg
     3901 cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg cgatccacgg
     3961 gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg gtgacattcg
     4021 cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt tgccgcacgt
     4081 cgtcaaccat gaactgccaa acgtaccgga agattatgtc caccgtatcg ggcgtaccgg
     4141 tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac acaaactgct
     4201 gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc cgggctatga
     4261 gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac gtggcggcat
     4321 caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtgaac
     4381 tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta cgccgcatcc
     4441 ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt
     4501 taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc gcaactccat
     4561 taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc agtaagcatc
     4621 gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca cacgcagttc
     4681 tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt taaccgactc
     4741 ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga ccatggggga
     4801 agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg cctgaaaata
     4861 gtgttcggag gtgggccagg tattcccgtc aaccttgatg ggcctggcgg caaagttaga
     4921 aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt gcatgacgtg
     4981 ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt attgcgagcc
     5041 gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct
     5101 gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg
     5161 ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc
     5221 ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg catctggcga
     5281 ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc atcgccattg
     5341 cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg caggatcaga
     5401 tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg
     5461 ctttcgggcg tgggcgctac gtttgtccac gtaatctgac ggcgctcgcc agcactgaac
     5521 ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat aatcaggaag
     5581 agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg gatggtctgc
     5641 gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt accgacaaag
     5701 ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt gttactcgtc
     5761 gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg
     5821 aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac gaaggtcatc
     5881 acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc gcgccctggt
     5941 atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc
     6001 cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt
     6061 atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc gggcaggagg
     6121 cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc tgccagcggc
     6181 tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac gatttaagtg
     6241 agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag atgaaccgcg
     6301 cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg gcgcaatctt
     6361 ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctacacctct
     6421 ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta
     6481 ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt tcgcgtttgc
     6541 aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct
     6601 ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag ccttccatcg
     6661 acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag gtggagagca
     6721 aaaaatatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag cgctttctcg
     6781 actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg cgttatcgct
     6841 tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg gtgggcttac
     6901 agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg catatccaca
     6961 aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa
     7021 gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc
     7081 agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt atctacgaca
     7141 aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg gtatttccga
     7201 tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac
     7261 gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa
     7321 tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc
     7381 gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg ggcggcgtat
     7441 tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt tacgaagcca
     7501 tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg attgtcatcc
     7561 ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg attctgctgc
     7621 ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact cgcgtgctga
     7681 cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg caggcgcagg
     7741 cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc ccgccgctgg
     7801 aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat accctggcga
     7861 aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt tcgcatccgg
     7921 aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg ttactaatgc
     7981 atggtactga aggcgaagtg tatgctaatc cgcagcgctg tccgcagatc aatctcattg
     8041 accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc gagttactgc
     8101 cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg gctggcagcg
     8161 aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag
     8221 cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt ttatctcagc
     8281 cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat
     8341 tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac ataaacagga
     8401 gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt ttattcaggc
     8461 tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat
     8521 cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa gctatgtgcg
     8581 ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta aagaggcggg
     8641 ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac acgaagcgat
     8701 ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc tacataactc
     8761 gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg ggtttgtctc
     8821 tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag
     8881 ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt tcccgctgtt
     8941 gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg
     9001 cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga ccaatccggc
     9061 ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata aaggctacgc
     9121 ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa cgacgcatca
     9181 ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta tcatcatcaa
     9241 cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt
     9301 gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt gggaagtgaa
     9361 cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct ggcaggccat
     9421 ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt tctgtcagca
     9481 gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga
     9541 tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg
     9601 acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg ataagacgcg
     9661 gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc cgtcctacga
     9721 attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg gaagtgatgc
     9781 tgtatccact ggcaccggct gccgccgctt tctcagccag tttcgcttcc agggcatcga
     9841 gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt gcctgggatg
     9901 ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc atagcagcaa
     9961 caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta taagtgacgt
    10021 tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg
    10081 accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg
    10141 tacttatgga cttgttttat gccgcgtggc agtcacagca gcgttaaagg cacggaatgc
    10201 cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa cgtaactgtc
    10261 tgtctttcga acctctgcga ttatatcctg tatacgctgg tttttgtaaa tctttctcct
    10321 gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg
    10381 gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca ccccgagcgc
    10441 gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc
    10501 aagcggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg tagctaatat
    10561 agggacgaac ctgggcagtt caagcgtcag atctccgacc attcccgcag cagattatga
    10621 taaagattga gcagcgacag gatctcttca ttttcaccat agcggctttt cagcgactga
    10681 atatttttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat catcgactgg
    10741 atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg
    10801 ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg tccaaaggtg
    10861 tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca
    10921 gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatgg
    10981 aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc
    11041 aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc attttgcagt
    11101 gcggcgtata acgcgctgcg ggtgtctact tgctgattgt tcttaacttg tgcaccttgt
    11161 gctccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaatgg
    11221 gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa
    11281 gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc
    11341 ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg taatcggtat
    11401 caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga taccccagtt
    11461 tggcatcggc gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg ccgtctgaac
    11521 ctttatgtat actgccgata tagcgtgcgc ccgcgccaac agagatatca tcggttgcct
    11581 gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat gaggaaccat
    11641 cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag ccgccaatca
    11701 cctgccacgc gggagtgata ttccctgcca cggatatctc atagccttcg acgcgtttct
    11761 taccgtattg cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg atatcagtac
    11821 ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg gtgccaatct
    11881 cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta ccactgccag
    11941 actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca tagttaatat
    12001 agacattgcc gttttccgtc aggtggtaca gcgccccggc tttccagttc accagattgc
    12061 ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt tgccacacca gctgggcagg
    12121 tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca gtatgataat
    12181 tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg
    12241 cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc
    12301 cgggatgaat tctgctgtca ggatgataaa tatttaccgc cggtaacgtc accggattaa
    12361 cgccatagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca tcatgaccga
    12421 tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt ttattactca
    12481 catctttggt attcgccgtg cgtgactagg tccagctatt cacatcgctg gtgggctgag
    12541 taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt acgcgcgacc
    12601 aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtgg
    12661 cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta tgagtatcaa
    12721 cttttccgga atgattcagg gccgccgctc ccgcagatgg ggcagaatag cccggcaaac
    12781 cgatggtcgg aatgccgccg tctggcgtgt tgtgctgagt gacatgcaga taattaagat
    12841 acaaacgatt tgctgtacca aggccaaaag cgacagaagg ggcgacgccg taacgctcat
    12901 ttttgacttt gtcgcgtccg gcatcgtggg ttttttcgcc catcacattc aggcgcaccg
    12961 cggtagtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac caggcgctgc
    13021 caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg atcatattga
    13081 tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga cggcccttta atcacttcga
    13141 cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaata ccatcaatat
    13201 aaatactgtt agaggtatcg gcaccacgca tataaatggc gtcgccagtg gtggagttac
    13261 cgttctcacc cgcaaaaaac gca
//
LOCUS       DAJTUN010000109        12897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTUN010000109 DAJTUN010000000
VERSION     DAJTUN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.109"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO470_003065"
     CDS             complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO470_003065"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6252242.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1796..3213)
                     /locus_tag="OO470_003066"
                     /pseudo
     CDS             complement(1796..3213)
                     /locus_tag="OO470_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(3367..3711)
                     /locus_tag="OO470_003067"
     CDS             complement(3367..3711)
                     /locus_tag="OO470_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6252243.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3711..4637)
                     /gene="glsB"
                     /locus_tag="OO470_003068"
     CDS             complement(3711..4637)
                     /gene="glsB"
                     /locus_tag="OO470_003068"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6252244.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4701..5636)
                     /locus_tag="OO470_003069"
     CDS             complement(4701..5636)
                     /locus_tag="OO470_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6252245.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5716..6831
                     /gene="yneK"
                     /locus_tag="OO470_003070"
     CDS             5716..6831
                     /gene="yneK"
                     /locus_tag="OO470_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6252246.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6981..8171
                     /gene="ydeA"
                     /locus_tag="OO470_003071"
     CDS             6981..8171
                     /gene="ydeA"
                     /locus_tag="OO470_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6252247.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8196..8861)
                     /gene="marC"
                     /locus_tag="OO470_003072"
     CDS             complement(8196..8861)
                     /gene="marC"
                     /locus_tag="OO470_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6252248.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9073..9507
                     /gene="marR"
                     /locus_tag="OO470_003073"
     CDS             9073..9507
                     /gene="marR"
                     /locus_tag="OO470_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6252249.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9527..9910
                     /gene="marA"
                     /locus_tag="OO470_003074"
     CDS             9527..9910
                     /gene="marA"
                     /locus_tag="OO470_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6252250.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9942..10160
                     /gene="marB"
                     /locus_tag="OO470_003075"
     CDS             9942..10160
                     /gene="marB"
                     /locus_tag="OO470_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6252251.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10191..11090)
                     /gene="eamA"
                     /locus_tag="OO470_003076"
     CDS             complement(10191..11090)
                     /gene="eamA"
                     /locus_tag="OO470_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6252252.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11354..12472
                     /gene="ydeE"
                     /locus_tag="OO470_003077"
     CDS             11354..12472
                     /gene="ydeE"
                     /locus_tag="OO470_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6252253.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12599..12694
                     /gene="mgtS"
                     /locus_tag="OO470_003078"
     CDS             12599..12694
                     /gene="mgtS"
                     /locus_tag="OO470_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6252254.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3397 a         3294 c         2949 g         3257 t
ORIGIN      
        1 caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata ggatgttgta
       61 actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta
      121 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
      181 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
      241 caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag
      301 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
      361 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
      421 gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
      481 accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt
      541 gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc
      601 ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac
      661 ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat
      721 tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata
      781 tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa
      841 gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac
      901 ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc
      961 ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac
     1021 cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga tgatccaacc
     1081 tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg tcagttttgc
     1141 cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc
     1201 ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc
     1261 atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct
     1321 gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt
     1381 gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc cagaattcag
     1441 atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc
     1501 ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag
     1561 tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt ggtgcgacag
     1621 gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca
     1681 gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg
     1741 gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga
     1801 caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat
     1861 acagacacgt atcggcttcg accatcaatt tattaaatca tcggtcagcg ttcggtacga
     1921 tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt gttgccaggt
     1981 gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac catcgacagg
     2041 attcaccgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca ccagcccctt
     2101 atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat cgccacattc
     2161 atgtccatag ttatcgttaa tgcttttgaa atagtcgata tcaagcagca tgaccgtcag
     2221 atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg accgtgagta
     2281 aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta gcaaattgat
     2341 cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa taccgagacg
     2401 tgcggagaac atttccggaa tggagaacgg cgaaccgacc gagatatcaa tcaccgaatt
     2461 tgccaccagt acgatttcca ccgcaccggt gacaaaggtc aacagacatg ttacctgcgg
     2521 cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac tccccgctcc
     2581 gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca tcatctgttc
     2641 tgctttaaag cgcggcagca ctccaggaat agccaacgtc agcatacaag gcacgatcaa
     2701 cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga aatccagact
     2761 atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg caatcagaca
     2821 gtaattaaat agccgtaaag cacttactgg ctcatactta tttttcccca gacgcttatc
     2881 ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat tgataaccag
     2941 tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca cataactgat
     3001 cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg ccattacgcc
     3061 attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac caataaaata
     3121 gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac cttcctggaa
     3181 caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac aattgcgggt
     3241 ggcaattatc tcaacgtgat aagtaaattc tcataaagga tagcatgttc gctaacagcg
     3301 gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt ctcatggctg
     3361 attcatctat acaaccagcc agtgatagat taccgccacg atataacgtt gttgttcttc
     3421 gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc gacagcaggt
     3481 cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt gcttaccgtc
     3541 attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat gttgttcgat
     3601 gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac gaaagcgaga
     3661 gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agatactgca ttaataaacc
     3721 gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag cgagttacct
     3781 gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac aatcgccaca
     3841 ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa ctcccccgcg
     3901 ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg cattggcgtc
     3961 accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac aaacgtccgg
     4021 gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg cagaacggtt
     4081 gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc cgcatttcgc
     4141 gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc agacacaccg
     4201 cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttagtcg cccttgcaac
     4261 atatcgcaga ccaccagcgc accggtatta atgaacggat tacgcggaat accctgttcc
     4321 atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc gacgcgttgc
     4381 cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac tttggaaata
     4441 gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc gtcaacggta
     4501 cagatagcaa tccccaatcg ggaaccgtct actgtagcca gcgccggaat ataatccgcg
     4561 actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa aattgcatta
     4621 tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc tgcgagtata
     4681 tcagagctga atatgtcgcg tcagatccgg tctttccaca ctgtctggat attacagaat
     4741 tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac gccaccaaag
     4801 gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca ttccagacgt
     4861 gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga aaggccgaac
     4921 tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt gattgcagca
     4981 acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt cgccagaacc
     5041 gttggcggat agtagttacc tgctccagcc atcttttccc cgcccagtaa caaacgcgca
     5101 ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc aaaacgagcc
     5161 attggtccga gagcgttctc ttcatcacgg ggatcgccca ttttcaaggc tgccgcagct
     5221 gacacaaaac gttcggtaaa tgccgaagca attccctctt cgataataaa gcgttttgcc
     5281 gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt aaccgccagt
     5341 tccagatcgg catcgttaag cacaataaat ggatccgaac cgcccagttc cagtacgcat
     5401 tttttcagtg ccgctccagc acgaacactt ccggtcaccg tgacagcagc aatgcgcgag
     5461 tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata tacgccttgc
     5521 gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat cacattcggc
     5581 gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc acgcatcacc
     5641 tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga tgacttatga
     5701 ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat
     5761 gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga ttttattgat
     5821 gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac caactcagcc
     5881 acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac ttcttttcgg
     5941 ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc tgtcaagaat
     6001 ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc tatttattta
     6061 gataattttc gccatgatgg agaaattaat attttttgcg ctcccaaccc aaagcctgtc
     6121 actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt tatgaatgat
     6181 tttattgaac aatttccaat gatcaacctt ggcgccccca tcaaaagagc gcatacacca
     6241 catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg ccagtttgat
     6301 ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg attatggaat
     6361 aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac cgccattctc
     6421 gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga ttcattaggt
     6481 tctaacaaac atgtacaacc cgaagtctgc atctgcttct tatccctttt agcagaacaa
     6541 gaatctcctc attttcagga cttgttttta tttttcgcca atatgttatt gcactatcac
     6601 caatttatga atcccaatga aagtgatctg aatgacgtgt tgatgccagc atcattaagt
     6661 gatgataaaa ttatcaaaca tatggcgcgc aggaccctca aactgtttgt aaaaaatgaa
     6721 acgccgccaa aagttactca cgaagacctg gtgaaaaaca ggcctcgctc ccctgtcaga
     6781 ccacctatac ccgcaaccgc caaaacgcca gacctccctg aacgtcatta aaccgtgatg
     6841 ttaccgactc tctgactcgg aacagaatca gcgtcagaga aacggaaaac gcgatccaga
     6901 tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc actttttcgc
     6961 aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg gcgtggctac
     7021 gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt gtccctgttg
     7081 gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc ggcatcatgt
     7141 tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta atgaccagtc
     7201 aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc agccacgtac
     7261 tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt gtggcttttg
     7321 cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct ccggccggga
     7381 agcgggcaca ggccttgagt ttaattgcca ccggtacagc actggcgatg gtcttaggtt
     7441 taccgctcgg gcgcattgtg gggcagtatt tcggctggcg aatgaccttc ttcgcgattg
     7501 gtattggggc gcttgttacc cttttgtgcc tgattaagtt acttccctta ctgcctagtg
     7561 agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca ttgatgagca
     7621 tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc tatatcgagc
     7681 cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta ctgttattac
     7741 tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat caatatgcgt
     7801 ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg tctggcactg ctgctacctg
     7861 cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc gcgatgatga
     7921 tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgct accgacgtcg
     7981 cgatggcgct attctccggc atatttaata ttggaatcgg ggcgggtgcg ttggtaggta
     8041 atcaggtgag tttgcactgg tcaatgtcga tgattggtta tgtgggcgcg gtgcctgctt
     8101 ttgctgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca ctcgaagaac
     8161 agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt aatgatttcc
     8221 aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaaccc catcaggcgg
     8281 gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccgga gctgcgtagg
     8341 cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac catcagtacc
     8401 caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat catcgctatg
     8461 gttcccggac cggcggtact tggcattgcc agtggtacaa aggcaatatt ggcactgggt
     8521 tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc tttctgttgc
     8581 ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat tcgcagaccg
     8641 ggaatcgaaa tgccaaatgt atccatcacc agttgcccag cgtaatacgc caccatcatg
     8701 atggcaaata cgtacaccga ggccatcaac gactgacgat tacgttcggc actgttcatg
     8761 ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg ggttagctaa cggcagcaac
     8821 accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg ttatcctgtg
     8881 tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc actctttagc
     8941 taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg catcggtcaa
     9001 ttcattcatt tgacttatac ttgcctgggc aatattaccc cctacaacta attacttgcc
     9061 agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc attgggtcgc
     9121 ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct gtctccgctg
     9181 gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc gtgtattact
     9241 ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg tatgctggat
     9301 cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc cgaatgataa gcgcggcgta
     9361 ctggtaaaac ttaccaccag cggcgcggaa atatgcgaac aatgccatca attagttggc
     9421 caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagttgc aacacttgag
     9481 catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt ccagacgcaa
     9541 tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc tggaatcgcc
     9601 actgtcactg gagaaagtgt cagagcgttc gggttactcc aaatggcacc tgcaacggat
     9661 gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta agatgacgga
     9721 aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag aacgatatgg
     9781 cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg ttccgccgca
     9841 taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat taaatcatta
     9901 caacagctag ttgataacgt gacaacgtca ctgaggcaat catgaaacca ctttcatccg
     9961 caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa accacgcagc
    10021 cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc atcgcaggaa caaccaccgt
    10081 ttgatttaaa tcacatgggt actggcagtg ataagtcgga tgcgctcggc gtgccctatt
    10141 ataatcaaca cgctatgtag tttgttctgg ccccgacatc tcggggctta ttaacttccc
    10201 acctttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc ggtcataatg
    10261 agcaccgcac ctaaaaattg cagacccgtt aagcgttcat ccaacaatag tgccgcactt
    10321 gccagtccga ctacgggcac cagtaacgat aacggtgcaa cccgccaagt ttcatagcgt
    10381 cccagtaacg tcccccagat cccataacca acaattgtcg ccacaaacgc cagatacatc
    10441 agcgacaaga tggtggtcat atcaatagta accagactgt gaatcatgga tgcggaacca
    10501 tcgaaaatca gcgagacaac aaagaaggga atgattggga ttaaagcgct ccagattacc
    10561 agcgacatca ccgccggacg cgttgagtgc gacatgatct ttttattgaa gatgttgcca
    10621 cacgcccaac taaatgctgc agccagggtc aacataaagc cgagcatcgc cacatgctga
    10681 ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc gatccccgcc
    10741 aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc caagcacgat agtaaaaaac
    10801 gcctgtgcct gtaacaccag cgaagccagt ccagcaggca taccaaagtt aatggcgcag
    10861 aacagaaagg caaactgcgc aaaactgatg gttaatccat accccaagag caaattcagt
    10921 ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag cgaccagcat aaagcgcaaa
    10981 ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac aaaatttagc
    11041 ccccatacga ccactaccag tagcgccaac accccatctt ttcgcgacat tctaccgcct
    11101 ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt taacagttga
    11161 ttcgttagtc gccggttacg acggcattaa tgcgcaaata agtcgctata cttcggattt
    11221 ttgccatgcg tccgatggtt aataacaacg acgaggcaag aagggcgctg gtagagcgtc
    11281 gtagggataa gttcatttgt ctgtccggca gtgcgtttcg ttatgtttta ttttagagat
    11341 gtaaagaaat agcatggcta cgctgccatt tatgaccatt tacttgagtc gccagtacag
    11401 cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg gcgtcgtttt
    11461 tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata tgttactggc
    11521 aattaccgcc ttcgccagcg gttttattgc cattccttta gtgaataacg tgacgctggt
    11581 tgtgctcttt tttgccctca ttaactgcgc ctattctgtt tttgctactg tgctgaaagc
    11641 ctggtttgcc gacaatcttt cgtccaccag caaaacgaaa atcttctcaa tcaactacac
    11701 catgctaaac attggctgga ccatcggtcc gccgctcggc acgctgttgg tgatgcagag
    11761 catcaatctg cccttttggc tggcagctat ctgttccgcg tttcccatgc ttttcattca
    11821 aatatgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg tctggtcgcc
    11881 gaaagtttta ttacaagata aagcactgtt gtggtttacc tgctctggtt ttctggcttc
    11941 ttttgtaagc ggcgcatttg cttcatgcat ttcacaatat gtgatggtga ttgctgatgg
    12001 ggattttgcc gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca tggtggttac
    12061 gttgcaatat tccgtgggcc gccgacttaa cccagctaac atccgcgcgc tgatgacagc
    12121 aggcaccctc tgtttcgtca tcggactggt cggttttatt ttttccggca acaacctgct
    12181 attgtggggt atgtcagctg cggtatttac tgtcggtgaa atcatttatg cgccgggcga
    12241 gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt tttccgccca
    12301 gtctttaggc tggcttggtg ccgcgattaa cccattagtg agtggcgtag tgctaaccag
    12361 cctgccgcct tcctcgctgt ttgtcatctt agcgttggtg atcattgctg cgtgggtgct
    12421 gatgttaaaa gggattcgtg caagaccgtg ggggcagccc gcgctttgtt gatttaagtc
    12481 gaacacaata aaaatttaat tcagccttcg tttaggttat ctctgctaat atctttctca
    12541 ttgagatgaa aattaaggta agcgaggaaa cacaccacac cataaacgga ggcaaataat
    12601 gctgggtaat atgaatgttt ttatggccgt actgggaata attttatttt ctggttttct
    12661 ggccgcgtat ttcagccaca aatgggatga ctaatgaacg gagataatcc ctcacctaac
    12721 cggccccttg ttacagttgt gtacaagggg cctgattttt atgacggcga aaaaaaccgc
    12781 cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt acgctaacag
    12841 gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc tcaatga
//
LOCUS       DAJTUN010000110        12882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTUN010000110 DAJTUN010000000
VERSION     DAJTUN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.110"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..191
                     /gene="torD"
                     /locus_tag="OO470_003079"
     CDS             <1..191
                     /gene="torD"
                     /locus_tag="OO470_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6252255.1"
                     /translation="FSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSFGFY
                     AALSQLLLVLVECDHQNR"
     gene            complement(284..589)
                     /gene="cbpM"
                     /locus_tag="OO470_003080"
     CDS             complement(284..589)
                     /gene="cbpM"
                     /locus_tag="OO470_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6252256.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(589..1509)
                     /gene="cbpA"
                     /locus_tag="OO470_003081"
     CDS             complement(589..1509)
                     /gene="cbpA"
                     /locus_tag="OO470_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6252257.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1770..3026
                     /locus_tag="OO470_003082"
     CDS             1770..3026
                     /locus_tag="OO470_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6252258.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3319..4560
                     /gene="agp"
                     /locus_tag="OO470_003083"
     CDS             3319..4560
                     /gene="agp"
                     /locus_tag="OO470_003083"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6252259.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4598..4825)
                     /locus_tag="OO470_003084"
     CDS             complement(4598..4825)
                     /locus_tag="OO470_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6252260.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(4846..5442)
                     /gene="wrbA"
                     /locus_tag="OO470_003085"
     CDS             complement(4846..5442)
                     /gene="wrbA"
                     /locus_tag="OO470_003085"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6252261.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            5815..5922
                     /locus_tag="OO470_003086"
     CDS             5815..5922
                     /locus_tag="OO470_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252262.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(6004..7332)
                     /gene="rutG"
                     /locus_tag="OO470_003087"
     CDS             complement(6004..7332)
                     /gene="rutG"
                     /locus_tag="OO470_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6252263.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7353..7847)
                     /gene="rutF"
                     /locus_tag="OO470_003088"
     CDS             complement(7353..7847)
                     /gene="rutF"
                     /locus_tag="OO470_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6252264.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(7858..8448)
                     /locus_tag="OO470_003089"
     CDS             complement(7858..8448)
                     /locus_tag="OO470_003089"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6252265.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8458..9258)
                     /gene="rutD"
                     /locus_tag="OO470_003090"
     CDS             complement(8458..9258)
                     /gene="rutD"
                     /locus_tag="OO470_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6252266.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9266..9652)
                     /gene="rutC"
                     /locus_tag="OO470_003091"
     CDS             complement(9266..9652)
                     /gene="rutC"
                     /locus_tag="OO470_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6252267.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9664..10356)
                     /gene="rutB"
                     /locus_tag="OO470_003092"
     CDS             complement(9664..10356)
                     /gene="rutB"
                     /locus_tag="OO470_003092"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6252268.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10356..11447)
                     /gene="rutA"
                     /locus_tag="OO470_003093"
     CDS             complement(10356..11447)
                     /gene="rutA"
                     /locus_tag="OO470_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6252269.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11735..12373
                     /gene="rutR"
                     /locus_tag="OO470_003094"
     CDS             11735..12373
                     /gene="rutR"
                     /locus_tag="OO470_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6252270.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(12667..>12882)
                     /locus_tag="OO470_003095"
     CDS             complement(12667..>12882)
                     /locus_tag="OO470_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6252271.1"
                     /translation="LEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMR
                     FTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         3267 a         3273 c         3260 g         3082 t
ORIGIN      
        1 atttttcgct gggagagggg accgttcctg cgcgaagaat cgacagtttg cggcaaaaaa
       61 cactgacggc gctgcggcaa tggttaccag agtttgctgc gcgttgtcga cagtatgaca
      121 gctttggttt ttacgcggca ctaagccagt tattgctggt gttagtggag tgcgaccacc
      181 aaaacagata acgtcgttta tgcgcctgaa aagacgcgtt tagcgtcgca tcaggcatta
      241 tggcgcagtt gccggatgcg tcatcacatc cggcaaacac acatcacgga tgagctacaa
      301 accgggaaag ccgctggcgt agcaggcggt tttcctgctt caggtgcgca atatcatcca
      361 ttaacgtcag cgccaccgcg atccccggcc agtccagagc cagttcatga cgcaggcgta
      421 ccgcgcgttg caccacaatg gcggcatggt cgtcaaatac ccaggttgtt tcctggatct
      481 cacgcggttc aaccaccccc aaaccgacaa tttcattcaa ctcctcttca gagatgccgg
      541 tatgcaggca aaattcggta atagtaaaag tcaccgtaac attagccatt atgctttccc
      601 ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc agttgctgcc acagcgcggc
      661 agtgttttca tccggtttcg gcggcatcac gattttcagt accgcataca gatcgccggt
      721 ctgttttttg ctcaccagac ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc
      781 tggcgggata gtcagcaaaa tgctttcttt cagtgttgga acggtgactt tagtacccag
      841 cgccgcttcc cacgggctaa ccggcaccac aatttccaga tcctggccga caatatcgaa
      901 cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg ccatttggac cgccgttttc
      961 gcccggcgtc ccctggcctt tcaggcggat gcgttgacca ttgccgacgc ctgccgggat
     1021 cttcacattc agcgttttcg ggatttcctg ttcgatcatg ccaaaggcgt tataaaccgg
     1081 caggttatag ctgatggtac gcttatgctc agtaagcgtt tcttcgagga ataccgccac
     1141 ttcgatttca atatcgtggc cgcgtgcggc ggggcgttga cggctctggc gggcatgctg
     1201 accgaaaatt gacgagaaga tatcgtcaaa atcttcggca ttaaaactct gaccgtcgcc
     1261 atgatggaac tgacggttaa attgcggatc gttgcgatgt tgccacatct gatcatactc
     1321 agcgcgacgt tgttcatcac ttaacacttc ccaggcttca gcgacctctt tgaagcgggc
     1381 ttcggcatcc ggttctttgc tgacatcagg atggtattta cgggccagtc gacgataggc
     1441 ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc atgatggcgt aataatcctt
     1501 taattccata gcgttatctc gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa
     1561 ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc aacacagaat atgttattga
     1621 aatcatcgcg gagaggaggt cgccatcaag atgggttgct gaacatattt taaacaggtg
     1681 aaaaagggtg agcgattttt gatagttgaa ccaggcactt taagtttaac tagggcgtca
     1741 ttatttatta aattttatag acgctatata tgggtagtaa tatacatgga attagttgca
     1801 ctgcaaataa ttatttgaaa caggcctgga acgatataaa aaatgagtac gaaaaaaatc
     1861 aaacatattc aatcacgctt tttgaaaaca cactggtgtg ttttatgcgg ttgtacaatg
     1921 aactcagacg taaagtaaat gaagaggata ctccatgtct ggaatgtgaa tcactagaaa
     1981 aagaatttga ggaaatgcag aatgataatg atctatcatt atttatgaga acattgcgta
     2041 ctaatgatac acaaatttat tcaggggttt caggaggtat tacatatact atacaatatg
     2101 ttcaagatgt tgatattgtt agagtgtcct tgccgggcag aggttcagag cctatcacag
     2161 attttaaagg ttattattgg tatggcttta tggaatatat tgaaaacatt aatgcgtgtg
     2221 atgatgtttt ttctgagtat tgtttagatg atgaaaatat gagtatccag ccagaacaga
     2281 taaatatgcc gggaatatct gatttagata ctggtattga tttgtctggc atatctttta
     2341 ttcagagtga aattaataaa acatatggat taaaatacgc tccagtagat ggcgatggat
     2401 attgtctgtt aagagccata ctggttttaa aagaacatga gtattcatgg gcgctgggca
     2461 gccataagac gcaaaagcaa gtatacgaag agtttattaa aattgttgat aaacaaacga
     2521 tcgaggctct tgttgatacg gcattctata atctcaggga agatgtaaag acattatttg
     2581 gcgttgatct gcaatctgac aacaaaattc agggtgtggg tagttttatg tcatggagct
     2641 ttctgttttt taaaaaacaa ttcattgata gttgcttgaa tgacaaaaaa tgcatactgc
     2701 atttacccga gtttatattt aatgataaca agaatttgct tgctttagat accgacacgt
     2761 cggataggat taaagcggtg aagaattttc ttgctgttct ttcagatagc atttgctcat
     2821 tatttattgt taatagtaat gtggcatcaa tctccttggg aaatgaatcc ttttcaacag
     2881 atgaagatct tgagtatggt tatttaacaa acactggcaa tcattatgat gtttacctcc
     2941 ctcctgaact tttttctcag gcttataagt taaaaaataa ggaaatgaat gcgcaacttg
     3001 actatttaaa tcgttatgca acttaatggc aaaggcatat gctaaaaacc attgttatta
     3061 gtctcacact tttttattgg taaatattgt ctctgtattg gtaacgccgc agatattctg
     3121 tttagccaca ggtgcaatta tcagcggcgt acgcgaggca ggggctaatc aggcatagtt
     3181 tgcgtcaaac cttgcctgtt tttgaagatg tatatagaaa aacaggcgtt caacaagcca
     3241 ttttgcgaac ctgttcccgg aaaaaagtca tatttctgtc acactcttta atgattgata
     3301 acaaaagagg tgccaggaat gaacaaaacg ctaatcgccg caactgtggc agggatagtt
     3361 ttactcgctt caaatgctca ggcacaaacc gtaccggaag gctatcagct acagcaagtg
     3421 ctcatgatga gccgccataa cttacgtgcg ccgctggcga acaatggcag tgtgctggag
     3481 cagtcgacgc cgaataaatg gcccgaatgg gacgtccccg gtgggcaact caccaccaaa
     3541 ggtggcgtgc tcgaagtgta tatgggccat tacatgcgtg aatggctggc agagcagggg
     3601 atagtgaaat cgggggaatg cccgccgccg gacaccgttt atgcctatgc caatagtctg
     3661 caacgtaccg tcgctaccgc ccagttcttt attactggtg cattccctgg gtgtgatatt
     3721 cctgtgcatc accaggaaaa aatgggcact atggacccaa cctttaaccc gatgatcacc
     3781 gatgattccg ccgcattcag tgaacaggcg gtggcggcaa tggagaaaga gctcagcaaa
     3841 ctccagctta ccgacagcta ccagctactg gaaaaaatcg ttaactataa agattcccct
     3901 gcctgtaaag agaaacaaca gtgttcgctg gtggatggca aaaatacctt tagcgccaag
     3961 tatcaacaag aaccaggtgt ttccgggccg ctgaaagtcg gcaactcgct ggtagatgcg
     4021 tttactttgc aatactacga aggttttccg atggatcagg tggcctgggg agaaatcaaa
     4081 tctgaccagc agtggaaggt attgtcgaag ctgaaaaacg gttaccagga cagtctgttt
     4141 acctcaccgg aagtggcgcg caatgttgcg aaaccgctgg tcagttatat cgacaaagcc
     4201 ctggtcaccg atcgcgccag tgctccgaaa atcacggtgt tagttgggca cgactccaac
     4261 atagcctctc tgttaacggc gctggatttc aaaccgtatc agttgcatga ccagaacgaa
     4321 cgcacgccga ttggcggcaa aatcgttttc cagcgttggc atgacagcaa agccaatcgc
     4381 gatttgatga aaattgaata tgtgtaccag agtgcggaac agttacgtaa tgccgatgcg
     4441 ttaaccctgc aggcacctgc gcagcgcgtg acgctggaat taagcggttg cccgatagat
     4501 gccaacggtt tctgcccgat ggataagttt gatagcgtgt tgaatgaggc ggtgaaataa
     4561 cagaaaactc ccccgcgaga agcgggggag tcgctggtta aacgttttta cgttcgatgg
     4621 tctgttcgcc ccaaaaaagc gaatctttat cggtcttagc aaaggctttg actaacactt
     4681 catcactacc ttcttcccaa atcttttctg ccattttttc gtcgtacccg gcgacttcga
     4741 aaatggcctc ggctatttcc ggcgacgtat tgcgcagaga tgcccattca ccgacgtgat
     4801 gagctttcgc ttcttgagtt ggcatgcgta tcctcctgtt gaagattagc cgttaagttt
     4861 aactgccaga cctgcgacat attccccttg ataacgagca atagacagtt cttcctggct
     4921 tggctggcgt gagccgtcac cgcctgcgat ggtggttgcg ccgtacggcg taccgccgcg
     4981 aacctgtgaa acgtcaaata attcctgcgc tgcgtagcca atggggacaa tcaccatgcc
     5041 gtgatgcgca agggtcgtcc aggtggatgt gatggtttgt tcctgaccgc cgccagtacc
     5101 ggtggaacta aagacgctcg ccagttttcc gtatagtgcg ccggaagccc acaggccgcc
     5161 cgtctggtcg aggaaggtac gcatttgacc ggacatgttg ccaaagcggg taggtgtacc
     5221 aaaaataatg gcgtcgtaat cggccagttc ttgcggggtt gcaaccggtg cagtttgcgt
     5281 tttaccgcct gctttttcaa ataattgcgg cggcatggtt tccggtacac gcttaacgac
     5341 aacttctgcg ccatccactt tgcttgcacc ctcagcgact gcgcgtgcca tcgtttcaat
     5401 atgtccgtac atggaataat aaagcaccag aactttagcc atttctaacc actcctcgtg
     5461 ttatctctat tccgtagcga ttcgctacca cttatttaaa gataagacgt ccttttcaga
     5521 gtgcaaattt cacaaccact tatttgattt ataacaactt tcacaagcac gtaattttgt
     5581 cgcaaaatga cacattttta tctcatcgcg tttttttaat cataagagcg gcttatggat
     5641 aattattgga gatgatatct attctcgcta agaagctgtt gcaggatatt accaaacgcg
     5701 ggtctgtccg cgtcagttca ctaagcttag tcccacgtag cgaaaatatg gcagccgcca
     5761 tacgccgcgt taattctatg caatatgatg tctataccca gacggaggtc agtaatggca
     5821 aaccatcgag gcggttccgg caattttgca gaagaccgcg aaagagcatc agaagcaggt
     5881 aaaaaaggtg gcaagagcag tcacggcaaa agcgacaact agccgggcta atcaatgacg
     5941 aatgcatttt tttcttaagc tcgtcaaaaa gtcatcaccg ccggtaaccc ggcggttttc
     6001 tgtttatggc tcctgatgaa caacttctgg cggtggaacg tcaaccaacc ttctgcttaa
     6061 taacgcattg agtaaaatcg cgccaaaggt tgctgtaccg atccctccca acgtaaaacc
     6121 gccaagcgtg agagcaaaat cacccgcgcc cagcacgaag gttactgcga ccataatcaa
     6181 attagcgttc tggcttaaat cgacacggtt ttgtacccat atccttgcgc ctgcgacggc
     6241 aatcagcccg aacacaacaa ttgatgcacc accaatcacc gcggccggaa tggtatggat
     6301 cagcgcgcca aatttcggcg aaaagcccaa cagcatagcg atgacggcag ctgcaacaaa
     6361 caccagcgtc gagtagactt ttgtcacggc catcacaccg atattttcag cataggtggt
     6421 gacgccgcta ccgccgacag agccggaaag catcgttgcc agaccatcac cgacgaatgc
     6481 tcgccccata tacgggtcca tattgcgtcc ggtcatcccg gcgaccgcct tgagatgacc
     6541 taagttttcc gccaccagaa tcaccgccac gggcgcaatc aacatcatcg cttggccatt
     6601 aaaaccagga gtggaaaaat gcggcagacc gaaccaggca gcatagctga cgagagtaaa
     6661 atcgacggct tttcccagcc ctaaaacgtt ggtcatcacg ccatacagca gacaggcgac
     6721 aattaatcct acgagaatca ataaccgctg gatcatgcca cgggtaaaca ccgccaccag
     6781 cccaatacac agcaccgtca ttaccgccat ccagctatca aaggccgaag ccgatacact
     6841 tttcactgcg ataggcgcta agttcaggcc aatcgccatc accaccgcac ccgtcaccac
     6901 cggcggcatt agtcgttcaa tccagcgtgt accaattttc atcaccacca ggccaatgac
     6961 ggtataaacc agcccacagg cgataatccc gcccagcgcg atgctgatat tcgggttaat
     7021 gccctgaccg ttaaagcccg tcgcggcaat caccacgccg acaaaagccg cgctggagcc
     7081 gagataactg gggacgcgcc cgccggtaat aaagaaaaac agtaacgtgc cgatccccga
     7141 cattaaaatg gaaagattgg gatccagccc catcagaatc ggcattaaca ccgtcgcgcc
     7201 aaacatcgcc accgcgtgtt gaacgcccat tactgccgtc tgagcaaacg gcaatcgttc
     7261 atccggcgcg accacgccgc tctctgtaga ggtcgatttt aactgccagt gaggaaaacc
     7321 gaacattgcc atcagctgtc tccttaagga ggttaacaag cagggcgcat cagcgcgtga
     7381 taactgcgat cgaaccacac cagcccgtag ggtgtggtgt gacgatgaat cgcttcgatg
     7441 gcgcaaaaca gaatgtcgtg ggtgccgacg ctcaccacct ggctgatacg gcagtcaaac
     7501 gaaaccagag cctcttccag ttgcgggcat ccggtcacac cagtctgcca gcgggcggcg
     7561 gcaaagcggt gttccatggg cgttttgccg ccaaaaagat ttgaaagcgg ctcctgcccg
     7621 gcgctcagtg tatttacaca cagcgttcga ttttcattga ataccggcca gacggacgcc
     7681 ccacgattca ggcacaccag taatgtgggc ggcgtgtcgg tcacgctgca gacggcgctg
     7741 gcggtgaacc cggcgcgccc ggctggaccg tccgtggtga tgatattgac cgccgcgccc
     7801 atacaggaca tcgcatcgcg aaaggtttgt tgatcaacta tgttcatagt ttgctcctta
     7861 caacagcccg caggcttctt caaaggacag acgtggcagg cgcgcaaaaa gtttgctgct
     7921 atcgccatag ccgatattaa tcagcagatt gctcttcagc gtgctgcccg caaaaaaggc
     7981 gtcgtcaacg tgttgacggt caaaacccga catcgggccg gtatccagtc ccagcgcacg
     8041 gcaagcgacg atcagataag ccgcctgcat ggaactgttg cgaaacgctg tttcttcggc
     8101 aagttgtggg ctggaggtaa accaactgcg ggcatcaccg tggggaaaca gtagtggtaa
     8161 ccgttcataa aattcactgt cccaggcgac gatagcggtg acgggcgcgg tcagggtttt
     8221 ttgcagattg ccgctggaaa gcgccgggcg cagacgttct tttccttctg cagtgcggat
     8281 aaacacgatc cgtgccggag aacagttagc tgatgtcggc ccccatttca tcagggcata
     8341 aagctcctgt aacgtctcat cgctgacggg tgtctcccgc cagccgttgt gagtgcgggc
     8401 atcggtgaac agggtgctaa gcgcacctgg gctaacggct tcgttcatag caattcctta
     8461 cagggcggct tcacggtgat gtaacaggct ggcaagcccg ttgagtaaca gagcattaaa
     8521 cgtttcggga tcggtcacgt tgcaggcgtg tccgccatag ggcatcacca ttttctggct
     8581 atcgggcagg gcggcatgaa gttcactgga acatgctgtt ggcaccagca gatcatcact
     8641 ggcgcagatg atttgcaccg ggcagcggat gcgatccgca tggtgactaa agtcagcgcg
     8701 tttgagggcg ttaagtcgac gtagtaaatt atttttgccc tgaaaatgcg ccagtgccag
     8761 cgcgtcttct gcctccaggc gaggtgcgcg ggccgccatc cagtcggcgg gatagaggaa
     8821 caacggctgc gcttccaccc atgcttgcgc gccgccgcta tacagtaatc gttcgcgaac
     8881 ctgaaaacag cggcgcgtat gggcgtttat tcgtagccag ccgttaacgc tgaccagcat
     8941 agttaccgac gcgggataat ccagcgccag ctgcatcccc accagcgcac cgagcgcatg
     9001 gccgaccacc gcgtaacgct caatccctgc ggctaccagc gcctgatgca gttccgctgc
     9061 catctgggcg atactgtaat cttctgccag tgtgtcggga ttattgccgg tgccgcgctg
     9121 gtcgtaacag actacctgat actcctgctc cagcaccgcc agttgcggta accagtaact
     9181 gccgctaccg ccaagacccg aaatcaacac cactacgggt gcatcagcat aagggggagg
     9241 tgagagtgaa agtttcatcg cggcctcact tggcgatatg cgcaattgtg gcgatttcca
     9301 ccagcgcgtc aggttttacc agtccgcact gaatgtagaa tcgcgccggt ttatcacccg
     9361 gaaaaaactc ggcgtagatt tcgttaatcg cggcgtaatt tttccagtcg gtaataaaga
     9421 tgctgttgaa ggtcacatcc gccatcgtgc cacccgccgt ctcgatcacc ttgcggatag
     9481 tttccagaac gtggcgggtt tgcgcctttg ggtcatcggc aaacagcacg ttattatgtt
     9541 gatcaaaagc cagcgtaccg gagacataca ccacgccatc agccagcgtg ccgggaacga
     9601 agggggccag cggtgcgctg ctgccagcgg gaataattac ggattttggc atcgctaaac
     9661 tccttaagcg atacgagcaa aggacgtggg agaaagcgcg tcgcagaacg tttcgacgtc
     9721 gctgacccag ccaaaaaagg tttcgatatt gaacaacgcg gctttctgtg caaattccgg
     9781 ccccgcctgg tgtgttgcgt cttccagcac cacgccgaaa tgctccagaa aaaagccatc
     9841 gcgtagcgtc gattcgacgc agacgttggt agcgataccg gtgaaaacca gatggcgtat
     9901 tccacggctg cgtaaaatgc tgtccagcgg cgtattgaag aaaccgctgt agcgcggctt
     9961 cggcagcaca atatcgccag gctgcggcac cagttcatcc accagttgat aatcccagga
    10021 gcctttcgcc agcaatttcc cctgcagctg cggctgctta cgcatggttt tcagggcgtt
    10081 cgatttatga aaattcggtg agccggggcc gccagcctcg acatactgtt catcccagcc
    10141 attttgaaac cagatgatca acatccctgc cgctcgcgct gcggtcacag cggtttgaat
    10201 gttggcaatg accgggcgag tggttgagac atcaaacccg gcgagatcta agtagccgcc
    10261 tggcgtggca taagcgtttt gcatatccac cacgatctgc gcactttgct gcggatcgaa
    10321 ggtaatggct tccggtcgag cggttaaggt tgtcatcatg ccacctcctg agtcagcgca
    10381 gagagatggg cgcggcactg catcagtggt tgaatgcgct cgccgaaggt ttcgattccc
    10441 gacagaaaat catcgaaggt caacagcacg ccttctgcac caggcacgct tgcgacttca
    10501 tctaacatgc gcgcgacact ggcgtaagaa ccgactaacg tccccatatt gatgtttacc
    10561 gccgaagtgg gatcggccat ctgacgaacg ttggtgtcag taccggagcg ggtatctttc
    10621 tgactttgtt cggttagcca gcttaacgcc tcttcatccg cgcccgcttt gtagtgttcc
    10681 catttggcgc gagtggcatc gtcggtttca tcggcaatca ccataaacaa cacataagag
    10741 ccaacgtcgc gtccggtttg ctctgtggcc tgtttcatcc gcgcagcggt cggggcgaaa
    10801 gccgtgggtg tatttacgcc tttgccgaaa cagaagttga aatcggcata ctgggcggag
    10861 aacgccatgc cagcgtcgct ttgcccggcg cagatcactt tcatggggat actcggttgc
    10921 ggactgacgc gacaatcatt catggtgaaa aaatcgcctt taaaatcgct tttccccgtg
    10981 ccccacaggt cgcgcagcac ctgaacatat tcggtgagat agtcgtaacg acgggagaaa
    11041 tagtcatcgc caggccagat acccatctgc tcatactcgg gcttttgcca gccagtcacg
    11101 aggttgacgc caaaacgccc gccagagatg gagtcgatgg ttgcggccat acgggcgacg
    11161 attgccggag gtaacgttaa ggtggcagca gtggcgtaaa tctgaatgcg cgaggtcacg
    11221 gctgccagac ccgccatcaa ggtgaacgac tcaaggttgt gatcccagaa ctcagttttg
    11281 ccgccaaagc cacgcagttt gatcatcgac agggcgaaat cgaaatggta atgctccgct
    11341 ttttgcgcaa tggctttatt cagttcaaag gtcggcatgt actgcggcgc gtgggtcgaa
    11401 atgagccagc cgttgttgcc aataggtacg aatacgccaa ttttcatcat caacctctct
    11461 tcgtctcgta aagtgaaagt cagacggggc gctgcatcct gcatatcctt ttcagccgcg
    11521 tattggcttg tttgcaaagc ggatgccagt ttttaaaaag tgaatgttat taatgtgtta
    11581 gcattgtgtt atctaaaata tctggtaaaa agtggactaa acggtcaaaa cagttgcaca
    11641 taaaacatgc atccgtgcgc gatgagagtg cagaaggtcg aggccgggcg ggggttttgc
    11701 tatcctgtta ccaatctaca agaggggaga gcgcatgacg caaggcgcag tgaaaacaac
    11761 gggtaaacgt tcgcgcgcag taagcgcgaa gaaaaaagcg attcttagcg cagcactgga
    11821 cactttttca caattcggtt ttcacggcac aagactggag cagaccgcag agttggcggg
    11881 tgtttcaaaa accaatctgc tgtattactt cccgtcaaaa gaggcgctgt atattgccgt
    11941 gctgcggcag attctcgata tctggctggc accgttaaaa gcgtttcgtg aagatttcgc
    12001 cccgctggcg gcgataaaag agtacatccg tctgaagctg gaagtctcac gcgattatcc
    12061 gcaggcttcg cgcctgttct gtatggagat gctggcaggc gcgccgttgt taatggatga
    12121 actgacgggc gatttgaagg cattaattga tgagaaatcg gcgctgattg ccggttgggt
    12181 caaaagcggc aaactcgcgc cgattgatcc gcagcatttg atttttatga tttgggcttc
    12241 cactcaacat tacgccgatt tcgcccctca ggtggaggcg gtgacaggcg cgacgttgcg
    12301 cgatgaggta tttttcaatc aaacggttga aaacgtgcag cggattatta ttgaggggat
    12361 tcgaccacgt taaagatgac ggaggaggtt gtaacatcct ccggctacct gtttaaccga
    12421 tcgtcattaa actggcgtta ccgccagcgg cagcggtatt cacactcagc gaacgttcat
    12481 cgataggaat taaaacccca aaaagattaa aaaaacacca caaaacggat gtttcttcaa
    12541 caccactttt gctccatatg aacggaaccg acgattaaac tggatggctc tgattgattc
    12601 agggtatgaa tggcggtttt ttgctccgtt tccctcaaaa tggacgcaac ttcccctctg
    12661 cggctctcag ccgcaccacc gcatccgggc cagcggctca tgcatcagga cctgctctgc
    12721 cagacggtag ccccgcttca gccccgtaaa acgcatctga ctcccgcaca gcacgcactt
    12781 cagcgggtca accttcagta acctctgata catccctctc caggtgattt gcatcgccgt
    12841 ttttctcact gtctccgtta tgatgtacac cacttcttcc ag
//
LOCUS       DAJTUN010000111        12882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTUN010000111 DAJTUN010000000
VERSION     DAJTUN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.111"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            181..2028
                     /gene="ldtD"
                     /locus_tag="OO470_003096"
     CDS             181..2028
                     /gene="ldtD"
                     /locus_tag="OO470_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6252272.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2209..2757
                     /locus_tag="OO470_003097"
     CDS             2209..2757
                     /locus_tag="OO470_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6252273.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2784..3431
                     /gene="gloC"
                     /locus_tag="OO470_003098"
     CDS             2784..3431
                     /gene="gloC"
                     /locus_tag="OO470_003098"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6252274.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3654..4844)
                     /gene="aspC"
                     /locus_tag="OO470_003099"
     CDS             complement(3654..4844)
                     /gene="aspC"
                     /locus_tag="OO470_003099"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6252275.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5029..6117)
                     /gene="ompF"
                     /locus_tag="OO470_003100"
     CDS             complement(5029..6117)
                     /gene="ompF"
                     /locus_tag="OO470_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6252276.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6718..8118)
                     /gene="asnS"
                     /locus_tag="OO470_003101"
     CDS             complement(6718..8118)
                     /gene="asnS"
                     /locus_tag="OO470_003101"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6252277.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8287..9489)
                     /gene="pncB"
                     /locus_tag="OO470_003102"
     CDS             complement(8287..9489)
                     /gene="pncB"
                     /locus_tag="OO470_003102"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6252278.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9755..12370
                     /gene="pepN"
                     /locus_tag="OO470_003103"
     CDS             9755..12370
                     /gene="pepN"
                     /locus_tag="OO470_003103"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6252279.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12577..>12882)
                     /gene="ssuB"
                     /locus_tag="OO470_003104"
     CDS             complement(12577..>12882)
                     /gene="ssuB"
                     /locus_tag="OO470_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="part of the ABC type transport system SsuABC for
                     aliphatic sulfonates; with SsuA being the periplasmic
                     substrate-binding subunit, SsuB the ATP-binding subunit
                     and SsuC the permease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6252280.1"
                     /translation="LLDEPLGALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVA
                     MADRVLLIEEGKISLDLTVDIPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
BASE COUNT         3276 a         3225 c         3261 g         3120 t
ORIGIN      
        1 cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt tatatactga
       61 agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt ggcgttgtac
      121 aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag ggggcaaggg
      181 atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg cctggccgta
      241 acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc tggcgacagc
      301 ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac ggcaataatg
      361 gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca aatcgaatct
      421 caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt gtatgccgca
      481 cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg
      541 gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga gttactgacc
      601 gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat gatgggctat
      661 ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag cagtaaacct
      721 tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc gctggataaa
      781 ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata tgcggcgatg
      841 catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact gaccggcaaa
      901 gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa
      961 cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact
     1021 gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac
     1081 aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta cgataatgaa
     1141 ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga tggtgctatt
     1201 ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggca
     1261 ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac
     1321 attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga ttcgcgagtc
     1381 attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa caacgtagtg
     1441 gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct gccgaaagtg
     1501 cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg ctggaacagc
     1561 agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc gaatttaccg
     1621 tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg
     1681 ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat
     1741 acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg
     1801 ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa gcagggtgat
     1861 acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt
     1921 gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg
     1981 cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc
     2041 ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc agcccccgtc
     2101 actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc
     2161 gccagaagtg catataaacg ataacattga gctgtagact tgattatcat ggacaaattc
     2221 gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg
     2281 ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat
     2341 cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa
     2401 gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc
     2461 gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg
     2521 gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg cgcccgcagc
     2581 cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa
     2641 ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga
     2701 tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagtaa
     2761 tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc gcattctccc
     2821 agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc gatcctggcg
     2881 gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc
     2941 tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg
     3001 gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc
     3061 aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg
     3121 aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc cctgggcata
     3181 cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta
     3241 ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt
     3301 cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt ccgggtcacg
     3361 gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa gacgaaatgc
     3421 ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat ttctggcaaa
     3481 gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca aaatcgtgca
     3541 aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca ggcaatgttg
     3601 cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa cgcttacagc
     3661 actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc ggccacattt
     3721 acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag cacttgttct
     3781 tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct gaagtcgcgg
     3841 tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg aatacgctgg
     3901 cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct caggatggtg
     3961 gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat cgccgctttc
     4021 atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt acaagcgcca
     4081 acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat cagctcttta
     4141 tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg ggcaaaaccc
     4201 tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg tgccagtgtc
     4261 tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc atggaacagc
     4321 actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa gtcaagggtg
     4381 tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga gttaaagacg
     4441 ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct ggtatttttt
     4501 gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg tgccgtgcga
     4561 gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg agtgcagcga
     4621 ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt ttcgagcaga
     4681 tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc atctttatag
     4741 acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag atcggccagg
     4801 cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag gttccattat
     4861 ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt ccaactatta
     4921 ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg cataaaaaaa
     4981 acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt agaactggta
     5041 aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt cagaatcgat
     5101 ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt aggttgcgcc
     5161 cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt tcgctttaga
     5221 tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg caactaacag
     5281 aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag tgatcggcgt
     5341 agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt cgtacttcag
     5401 accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt cttgcaggtt
     5461 ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt cgtagctgat
     5521 agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt ttttacccag
     5581 gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt tacgataggt
     5641 agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat caccaccaaa
     5701 ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt tacggccgta
     5761 atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg ttttgttacc
     5821 agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc actgaccata
     5881 accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg cataggtcat
     5941 gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat gcagaccaac
     6001 agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg cagcgtttgc
     6061 agtacctgct actaacagag cagggacgat cactgccaga atattgcgct tcatcattat
     6121 ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt tctgtcaata
     6181 aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc
     6241 attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt agtttcaaaa
     6301 agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg tcatatttcc
     6361 atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta cttatattta
     6421 aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc acaaaatcct
     6481 ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa
     6541 actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa tgtaatattc
     6601 cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta acaaacagat
     6661 aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca
     6721 gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc
     6781 agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc
     6841 gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat
     6901 acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga tctcaccgat
     6961 gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata
     7021 gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc
     7081 cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa
     7141 cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc
     7201 ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa
     7261 tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc
     7321 agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa
     7381 ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata
     7441 aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa
     7501 agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag
     7561 gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc
     7621 ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc
     7681 ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag
     7741 gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc
     7801 tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa attgttgccc
     7861 ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg
     7921 caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa
     7981 gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg
     8041 tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc
     8101 tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc
     8161 cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac aagcagaatt
     8221 cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat tagctccctg
     8281 atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac
     8341 aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt
     8401 accgttacac tctaccaact taatcacaat attcaggggt tttacctggg ggatatcgca
     8461 ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag
     8521 ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac tctgtggatc
     8581 aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga
     8641 gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac gcaggaaagc
     8701 atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact cttccagcca
     8761 ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct gatgtgcctg
     8821 gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta
     8881 gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac
     8941 ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg acatatcaag
     9001 accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc
     9061 aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg
     9121 aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca gcttgccatt
     9181 atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc agttaagata
     9241 gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct gcaggcgcag
     9301 gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc
     9361 acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg
     9421 ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa
     9481 ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa taaacatctt
     9541 caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc
     9601 cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt
     9661 ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa
     9721 caccagactc tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata
     9781 ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct
     9841 cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga
     9901 tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga
     9961 gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta atttgccgga
    10021 gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg cgctggaagg
    10081 gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt tccgccatat
    10141 tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa ttattgccga
    10201 taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag gcgaactcga
    10261 aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct acctgtttgc
    10321 gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt ctggtcgcga
    10381 agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt gggcgatgac
    10441 ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt atgacctcga
    10501 catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga ataagggtct
    10561 gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg acaaagatta
    10621 cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg gtaaccgagt
    10681 gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct tccgcgatca
    10741 ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg tgcgcaccat
    10801 gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc gcccggacat
    10861 ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg cggaagtgat
    10921 ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc aactctattt
    10981 cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga tggaagatgc
    11041 gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta cgccggttgt
    11101 gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca tcagccagcg
    11161 cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt ttgccatcga
    11221 attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc cggtgaattc
    11281 cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct acttccagcc
    11341 ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata agtggagcga
    11401 tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt gggatgcggc
    11461 gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc aagggcagcc
    11521 gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg atgagaagat
    11581 tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa tggctgaact
    11641 gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca ctcgtactct
    11701 ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc agagcgagta
    11761 ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc tgcgcttcct
    11821 tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc atgaagcaaa
    11881 caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc tgccttgtcg
    11941 tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg tgatggataa
    12001 atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg tgcgtggtct
    12061 gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt tgattggtgc
    12121 gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt accagttcct
    12181 ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac gtctgattga
    12241 accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc gcgcggcgct
    12301 ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga taactaaagc
    12361 actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt cttatcaggc
    12421 ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac gccgcatccg
    12481 gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc tacaagtccg
    12541 ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc gaatcagttg
    12601 ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct ccgctaatcg
    12661 caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac taatttttcc
    12721 ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat catgcgtcac
    12781 caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct gcatctcgag
    12841 tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aa
//
LOCUS       DAJTUN010000112        12865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTUN010000112 DAJTUN010000000
VERSION     DAJTUN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.112"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..144
                     /locus_tag="OO470_003105"
     CDS             <1..144
                     /locus_tag="OO470_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6252281.1"
                     /translation="FGFVIWLVVHRLLRGWIYAGDIWHPLLMDLSLFAICVCLALAIL
                     IAW"
     gene            147..1004
                     /locus_tag="OO470_003106"
     CDS             147..1004
                     /locus_tag="OO470_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6252282.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1004..3001
                     /locus_tag="OO470_003107"
                     /pseudo
     CDS             1004..3001
                     /locus_tag="OO470_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(3002..3523)
                     /gene="sodC"
                     /locus_tag="OO470_003108"
     CDS             complement(3002..3523)
                     /gene="sodC"
                     /locus_tag="OO470_003108"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6252283.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3604..4500)
                     /locus_tag="OO470_003109"
     CDS             complement(3604..4500)
                     /locus_tag="OO470_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6252284.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4549..4788)
                     /locus_tag="OO470_003110"
     CDS             complement(4549..4788)
                     /locus_tag="OO470_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6252285.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            4891..5490
                     /gene="nemR"
                     /locus_tag="OO470_003111"
     CDS             4891..5490
                     /gene="nemR"
                     /locus_tag="OO470_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6252286.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5527..6624
                     /gene="nemA"
                     /locus_tag="OO470_003112"
     CDS             5527..6624
                     /gene="nemA"
                     /locus_tag="OO470_003112"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6252287.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6705..7112
                     /gene="gloA"
                     /locus_tag="OO470_003113"
     CDS             6705..7112
                     /gene="gloA"
                     /locus_tag="OO470_003113"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6252288.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7215..7862
                     /gene="rnt"
                     /locus_tag="OO470_003114"
     CDS             7215..7862
                     /gene="rnt"
                     /locus_tag="OO470_003114"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6252289.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            7955..12413
                     /locus_tag="OO470_003115"
                     /pseudo
     CDS             7955..12413
                     /locus_tag="OO470_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(12457..>12865)
                     /locus_tag="OO470_003116"
     CDS             complement(12457..>12865)
                     /locus_tag="OO470_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6252290.1"
                     /translation="DLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQL
                     EYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARN
                     ILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         2894 a         3478 c         3435 g         3058 t
ORIGIN      
        1 tttggattcg tcatctggct tgtcgtacac cgcctgcttc gtggctggat ctacgccggc
       61 gatatctggc atcccttgtt aatggattta tcgctgtttg cgatttgcgt ttgccttgct
      121 ctggcaatac tgattgcgtg gtaactatgt caattaaaac aattaagtat ttctcaacaa
      181 tcattgtagc gatagttgcg gttcttgccg gatggtggct gtggaattat tacatgcaat
      241 ctccgtggac acgcgacggg aaaatacgcg cagaacaggt ttctatcacc ccacaggtgt
      301 cgggacgtat tgttgagctg aatataaaag acaaccaact ggttaacgcg ggggaacttc
      361 tgctcacgat cgataaaacg ccctttcaga tcgccgagct gaacgcacag gctcagctgg
      421 cgaaagcgca atctgacctc gcaaaagcca ataacgaagc gaatcgtcgt cgtcatctct
      481 cacaaaattt tatttttgcc gaagaactcg ataccgctaa cctcaatgtt aaagcgatgc
      541 aggccagtgt taatgccgca caggcgacgc tgaaacagac acaatggcaa ctggcgcaaa
      601 cggaaattcg cgccccggtg agtggatggg tgactaacct cactacccgc atcggcgatt
      661 acgctgacac cgggaaaccg ctgtttgccc ttgtcgacag ccactcgttt tacgtcattg
      721 gttattttga agaaaccaag ttgcgccata tccgcgaagg tgcaccagca caaattaccc
      781 tctatagtga taacaaaacg ttacagggtc acgtttccag tatcggtcgg gcgatttatg
      841 atcagagcgt tgaaagtgac tcaagcctta ttccggatgt gaaacctaac gtcccctggg
      901 ttcgtctcgc ccaactcgtt cccgtccgct ttgcgctgga taaagttcca ggcgatgtca
      961 cgctggtgtc gggcaccacc tgtagcattg ccgtaggtca ataatgaacg catcgtcatg
     1021 gtccttgcgc aatttgccct ggttcagggc cacgctggcg caatggcgtt atgcgttacg
     1081 caataccatt gccatgtgtc tggcgctgac ggttgcctat tatttgaatc tggatgaacc
     1141 ctattgggcg atgacctcgg ctgcagtggt tagctttccc accgttggcg gtgtcatcag
     1201 caaaagcctc ggacgcatgg ctggcagttt actgggagcc attgcggcac tgcttcttgc
     1261 cggacatacg ctcaatgagc cgtggttttt tctattgagc atgtcggcgt ggcttggctt
     1321 ttgtacctag gcctgtgcgc acttcacgaa taacgtcgcg tatgcatttc aactggcggg
     1381 ctacacggct gccatcatcg cctttccgat ggttaatatt actgaggcca gccagctgtg
     1441 ggatatcgct caggcgcgcg tttgcgaggt gatagtcggt attttgtgcg gcggcatgat
     1501 gatgatgatc ctgcctagca gttccgatgc tactgccctt ttaactgcat tgaaaaacat
     1561 gcacgcccga ttactggaac atgccagttt actctggcag cctgaaacaa acgatgccat
     1621 tcgtgcagca catgaggggg tgattgggca gatactgacc atgaatttgc tgcgtatcca
     1681 ggctttctgg agccactatc gttttcgcca gcaaaacgcg cgccttaatg cgctactcca
     1741 ccagcaatta cgtatgacca gtgtcatctc cagcctgcga cgtatgttgc tcaactggcc
     1801 ctcaccgcca ggtgccacgc gagaaattct cgaacaattg ctgacggcgc tcgccagttc
     1861 gcaaacagat gcttacaccg tcgcacgtat tatcgccccg ctacgcccga ccaacgtcgc
     1921 cgactaccgg cacgtcgcct tctggcagcg actacgttat ttttgccgcc tttatctgca
     1981 aagtagtcag gaattacatc gtctgcaaag cggtgtagat gatcatgcca gactcgcacg
     2041 gacatccgct ctggctcgtc ataccgacaa cgccgaagct atgtggagcg ggctgcgtac
     2101 attttgtacg ttgatgatga ttggcgcatg gagtattgca tcgcaatggg atgccggtgc
     2161 caatgcatta acgctggcag caattagctg cgtactctac tccgccgtcg cagcaccgtt
     2221 taagtcgttg tcactgctga tgcgcacgct ggtgttactt ttggtcaaat ttggcctgat
     2281 ggtccagatt agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat
     2341 gcagcttctt aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt
     2401 tatgggttcg tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa
     2461 ctataatctg gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg
     2521 tccaggatcg gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt
     2581 cgatcagcta agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca
     2641 tcacgtcagt cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg
     2701 gggtgtagtg ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg
     2761 ttccgatccg ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag
     2821 tgagcgtggc gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg
     2881 cgacagcctt gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg
     2941 gctgtactgc tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta
     3001 attacttaat tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat
     3061 cggacatatt atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg
     3121 atttcagacg aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg
     3181 caggcagatc gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg
     3241 gatcaagatg cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct
     3301 ggcagcttcc tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat
     3361 cgggtgaaaa ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc
     3421 caaccccttg cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg
     3481 tcgcaacaac cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg
     3541 tgcattcctg aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag
     3601 tgcttacggt acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt
     3661 cattttcagt gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat
     3721 aattggcagc ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga
     3781 gcccgcgttt aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc
     3841 atcattaaac agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg
     3901 ttgtagttgg tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac
     3961 ctgattagtg gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt
     4021 aaagttcgat acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc
     4081 cgccacttca tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg
     4141 atcggtcgcg agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt
     4201 gatgtaatga ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac
     4261 gatttccatc cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc
     4321 gcactgatag ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg
     4381 ctcttcaata aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta
     4441 gcccatcaca aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat
     4501 aatcgcttcc tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga
     4561 tggttgctcc ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg
     4621 ctgccgacaa aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa
     4681 acgctcatcc cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat
     4741 tccccggcac gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc
     4801 agataagatt attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac
     4861 tagaccgact ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc
     4921 gaacatctct tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg
     4981 ctaagcgaat tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc
     5041 tctaaagaag cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga
     5101 ctggctgagt tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac
     5161 cagcaaacac tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa
     5221 ctctctgccg aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct
     5281 cgcggcgtga tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta
     5341 accttttgtg gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg
     5401 aatctgcagg ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta
     5461 aaaaacatta ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga
     5521 gcctttatgt catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca
     5581 aaccgtattt ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct
     5641 accccgttga tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa
     5701 gccacacaaa tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg
     5761 gagcaaattg ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg
     5821 gccttgcagc tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag
     5881 gcaccggtag cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat
     5941 ggtcaggcga tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg
     6001 ggtatcgtca atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg
     6061 gtagagctcc actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac
     6121 catcgtaccg atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg
     6181 gtcgatgccg ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc
     6241 ggtactttcc agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt
     6301 gaacaactgg gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg
     6361 ggtgaaccgt atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt
     6421 atcggcgcag gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt
     6481 gatgcggtgg catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag
     6541 ctcaaagctg agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc
     6601 tataccgatt acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac
     6661 taaaacaaca ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata
     6721 ccatgctgcg cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca
     6781 tgaaactgct gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct
     6841 acggcccgga aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat
     6901 acgaactcgg cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt
     6961 gcgaaaaaat ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg
     7021 gtactacggt tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag
     7081 agaaagacgc cggtcgcggt ctgggcaact aatctcctgc cgggcgtgaa ctcatcgcgc
     7141 ccgcatcttt actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt
     7201 attaagagaa tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg
     7261 gtttttatcc tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc
     7321 tgcttgagat tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca
     7381 ccacattaca tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg
     7441 ccttcaacgg cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc
     7501 tgcacgaaat ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca
     7561 ttatggtggc gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg
     7621 cctcgctgaa acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg
     7681 ggctggcact cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg
     7741 acagcaccca ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg
     7801 aaatcgtcaa ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt
     7861 aatcgagtcg atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac
     7921 gcatcaagca tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc
     7981 gctcctgccg gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca
     8041 gccgaccgct gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt
     8101 gattgcaccg actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct
     8161 cttccgcgaa ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct
     8221 ctatatttca ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt
     8281 gaagggtatt gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca
     8341 cgcggtagcg ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc
     8401 gctgctccac acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga
     8461 tgtggcagca tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccgcaatgcg
     8521 ccatccgcag atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc
     8581 cagcggcacc ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga
     8641 aacgggtatc cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg
     8701 cgggctggcg gaaaaactga cagcacgatt aaatgagctt tacgccgcac gcttacagcg
     8761 ttccccgtct atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa
     8821 ccgtgtacaa agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga
     8881 acaacgagca atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac
     8941 ctccagtctt gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac
     9001 gccgctttct gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg
     9061 tgtatctaaa gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat
     9121 tgtagagtgt atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga
     9181 cgtccttgcg cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg
     9241 gtactcccgc gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc
     9301 cacgctggat atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa
     9361 actggtctgg aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc
     9421 ggttaccagc ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg
     9481 tgaagaaaag gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc
     9541 gcgggtgaac gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg
     9601 cgatcaggtg attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg
     9661 tgaaggtaac ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact
     9721 ggcggatggc gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa
     9781 cgccaatatt caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag
     9841 tcgccatctg gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca
     9901 ctctccctat ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca
     9961 tgccctatgg ggcgctgacg cgtctgtggt cgccagtgat gacggcattg ttgcgcgcat
    10021 tcctgacact gatggtaaat tgcccgacgc cgcgattttt ttgtttgaac cagaaaagtt
    10081 gctgcaaatt gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga
    10141 atgcgccgcg cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg
    10201 gcaacaacgg ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc
    10261 ggtcattctc gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga
    10321 acgtttgatg cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac
    10381 gccctcgcct ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag
    10441 cgacgccccg ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg
    10501 caatctactc ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt
    10561 ggaagaagag ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt
    10621 cgacctgctg cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg
    10681 cagcagtgaa gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc
    10741 agcgatgatt agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga
    10801 tgccctcggc gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta
    10861 cccgcttcgc gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca
    10921 actggctcat gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg
    10981 tgaacagggt ctggtgatga atctgcaaca aaacatctgg gtgagcgatg aagtatttcg
    11041 tcgtctgcgt ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac
    11101 gacctatgcg cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc
    11161 ggcgctcttt gcctcaacat ctccaggcgt ttatgagggc gtagatggcg tgatgcgggt
    11221 gatcgaacag cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc
    11281 tgcccgcgta cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt
    11341 tatctggtcg ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac
    11401 agaaatatgc cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc
    11461 tgcagcaagc gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca
    11521 gccaacggat acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt
    11581 tatgggcgct ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc
    11641 tcacccgcag cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg
    11701 ggcgtcctgt ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg
    11761 ctggacgctg gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc
    11821 tggcggaaaa tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa
    11881 atatccctgg cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc
    11941 gaattatgcg aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga
    12001 ctattgaccg attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag
    12061 tggcgctctc cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tggcctgcac
    12121 atccggcaac gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca
    12181 aattgttact ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg
    12241 aattactcgc cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc
    12301 tgcgctttac gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc
    12361 tgctgcgtga ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat
    12421 cctccacgcc ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc
    12481 ggtgggttcc tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc
    12541 gtgccccgcc gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca
    12601 tacctggcat ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca
    12661 cgcgcaacgc tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cactccagag
    12721 ctgcttgtac tcaagctggc ggcgcatttc ataccagccc tgatccagta tcgaacggtt
    12781 taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg ctgctgactt
    12841 tgacatgttg ctgaccttca aatcc
//
LOCUS       DAJTUN010000113        12489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTUN010000113 DAJTUN010000000
VERSION     DAJTUN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.113"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..726)
                     /locus_tag="OO470_003117"
     CDS             complement(<1..726)
                     /locus_tag="OO470_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6252291.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDQAEPHTEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKEEGRL"
     gene            complement(742..1074)
                     /gene="cusF"
                     /locus_tag="OO470_003118"
     CDS             complement(742..1074)
                     /gene="cusF"
                     /locus_tag="OO470_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6252292.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1098..2480)
                     /gene="cusC"
                     /locus_tag="OO470_003119"
                     /pseudo
     CDS             complement(1098..2480)
                     /gene="cusC"
                     /locus_tag="OO470_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            2637..3320
                     /gene="cusR"
                     /locus_tag="OO470_003120"
     CDS             2637..3320
                     /gene="cusR"
                     /locus_tag="OO470_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6252293.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3310..4752
                     /gene="cusS"
                     /locus_tag="OO470_003121"
     CDS             3310..4752
                     /gene="cusS"
                     /locus_tag="OO470_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6252294.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4902..7139
                     /gene="nrfB"
                     /locus_tag="OO470_003122"
                     /pseudo
     CDS             4902..7139
                     /gene="nrfB"
                     /locus_tag="OO470_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7126..10094
                     /gene="nfrA"
                     /locus_tag="OO470_003123"
                     /pseudo
     CDS             7126..10094
                     /gene="nfrA"
                     /locus_tag="OO470_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10095..10985
                     /locus_tag="OO470_003124"
     CDS             10095..10985
                     /locus_tag="OO470_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6252295.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11168..11929
                     /gene="envY"
                     /locus_tag="OO470_003125"
     CDS             11168..11929
                     /gene="envY"
                     /locus_tag="OO470_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6252296.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(11975..12051)
                     /locus_tag="OO470_003126"
     tRNA            complement(11975..12051)
                     /locus_tag="OO470_003126"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12015..12017),aa:Arg,seq:tct)
     gene            12294..>12489
                     /locus_tag="OO470_003127"
     CDS             12294..>12489
                     /locus_tag="OO470_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6252297.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGF"
BASE COUNT         3029 a         3257 c         3186 g         3017 t
ORIGIN      
        1 caacctacct tcctctttcg cgaccacgtt atctttggcg atattcattc ccgcgcgcag
       61 atcaaacgcg gtgatcacgc catcaatggg cgctttgagc gtaaagcgag tctggatttt
      121 ttgcgtggcg atcaggtggc gaatatccgc ctccggcatt cccgccagtc gcagccgctc
      181 aagaatgcct tcagtctggg tcgccgtacc gccggtttcg cgcaacagta aatactcact
      241 ctgcgcttcc acccagtcgg gaatggtcag gtcgagaagc ggtgtgccct tttgtacttt
      301 atcgcccacg gtaagcggat acaccttgtc gataaacccg gcagcgcgag cctgcacaat
      361 ggcatactga tactcgttgt aactgacatt cgccgggaaa ctctgggcaa aagtcagcgg
      421 cccgcgcgtg acggtagccg ttttcacccc cagattctgc gtctgagtcg ggtcaatgcg
      481 cacaccagac gcagaactct cttcatcggc atatttcggc accagatcca tatccataaa
      541 cggcgattta cctggtttat cgaaccgcgt attgggatac atcgggtcgt accagaataa
      601 gattttacgt tctgcggtcg gcattttttc tgtgtgcggt tccgcctggt caaaccaggt
      661 aaaacctgcc gctgaaataa taccgcccgc gatcatgctg ccgataataa gcgcgatttt
      721 tttcatctca ttaaacctgg gttactggct gactttaata tcctgtaata aagaaaggtt
      781 gccctgctgg acaaaattaa acgccacttt gtcgccggtt ttaatttcac tcattttcgt
      841 ctgcggggtg atggtaaagc gcatggtcat ctccggccag ttgacggcag caatcggatc
      901 gtgatggatg gtgatttttt tgctttccag atcaacacct tttaccacac cggtggcact
      961 aatgacctgt ggttgtgctt cgctcatggt ttcatgatga tgttcgttag cctgggcatt
     1021 aaagccaata acggtaaaca gactgaacat tgcgacttgc agtgcttttt tcatttattc
     1081 tctcctggag ttaaaaatta ttgctgccaa ccgcccccta aggcggtata caaataaatt
     1141 tcgttaacct gacgggcata attcaggtca agtaaagttt gtcgggttgc aaataaagaa
     1201 cgctcggcat ccagcacttc cagataactt accgcgccgt gctgatataa tgcccgcgcc
     1261 cgttgcaggg taatttgcag cgacgccaga taacgctgct gggcgctgat ttgatcgtcc
     1321 aggctttgac gtaatgccag cgcatctgcc acctctttaa aggcgttctg gattttctgt
     1381 tcataattca ccaccgactg ctgctggcga atttcggcga tatccagatt ggcctggttg
     1441 cgtccggcat taaaaatggg gatctcaatt ttaggaataa aattccacat cccgctgcta
     1501 gcgttaaata atgacgacag atcgctgctg gcggttgata ttccgctggt caggctgata
     1561 gacgggaaaa atgccgcacg cgcagcacca atattagcat tagccgccat taacgcgtgt
     1621 tcagcttcca taatatccgg gcgctgcaat aagatttgcg acgacaagcc cgccggtaat
     1681 ttaacgcttt gcaggctgcc gctgtttact gtctgcgctt gcggtagctt gccgtagctt
     1741 cccaataaca gttgcaatgc attattcgcc tgcgccagtt ccccctgacg tttagcgatg
     1801 tcactgcggg tactttctat caccccgcga gcctgttcca gcgccagaac attgctgcta
     1861 ccggtcaaca gttgtttttc gacaaacgca tatgactgct gataattacg cagcgtttct
     1921 tcggctattt gcaattgcgc atacgccagc tgctgattga aatagctttg cgcgacatta
     1981 gaaaccagca aaatatgcac cgcgcgctgg gcttcctcag tggctaaata attttggcgc
     2041 tcggcttcgc tcatgttctt taagcgaccg aaaaaatcga gatcaaagct ggcgttaagg
     2101 ccagtcgaga actcccgcgt cgtggctgaa tcgcctttaa gattgccgct ccagctgccg
     2161 ctgccctagc cattgagctg tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgt
     2221 gcctcctgca ctttcagcgt cgccatgcgc aaatcccggt tattcaccag cgcctcgcta
     2281 atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc agcccacgtt ctggtagtta
     2341 tccgctgcgt taaccaggcc gttctggctg agtgagaact gctgcggtac gggcattacc
     2401 ggacgctgat aatccggtgc cagtgaacaa ccagttagcg caagggccac acaaaatggc
     2461 agaagtttac aaggagacat aggctcataa tttctggtga ttttatgtcg ccaactttac
     2521 tcgccaggct ctgattttcc ggtgacagga aaatgacaaa attgtcattt tgccaataag
     2581 cgattgccat ctgatcccgc tactctagaa ttgcccgggc aacatgcgga ggaaatatga
     2641 aactgttgat tgtcgaagat gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg
     2701 aagccggttt tgtggtcgat ttggccgaca acgggctgaa tggctaccat ctggcgatga
     2761 ccggtgatta tgacctgata atcctcgata ttatgctgcc agacgtgaac ggctgggata
     2821 tcgtgcgcat gttgcgctcc gccaataaag ggatgccgat tctgttgctt accgcgcttg
     2881 gcaccattga acatcgcgtc aaggggctgg agttgggggc agatgactac ctggtgaaac
     2941 cgttcgcttt tgctgaactg ctggcgcggg tgcgcactct cctgcggcgc ggggcggcgg
     3001 tgattatcga aagtcagttt caggttgccg atctgatggt cgatctcgtc agccgcaaag
     3061 tcaccctcag cggcacgcgc atcactctga ccagtaaaga atttactctg ctggagtttt
     3121 ttctccgcca tcagggcgaa gtgctgcccc gctcgcttat cgcctcgcag gtatgggaca
     3181 tgaatttcga cagcgacact aacgccattg atgtggcggt gaagcggcta cgcggcaaaa
     3241 tcgacaacga ctttgagccg aagctgattc aaaccgtgcg cggcgtgggt tacatgcttg
     3301 aggtgccgga tggtcagtaa gccatttcag cgcccgtttt cgctggcaac ccgcctgacc
     3361 ttttttatca gcctggccac catcgcggcg tttttcgcct ttgcatggat cataatccac
     3421 tcagtaaaag tgcattttgc cgagcaggac attaatgatt taaaagagat tagcgccacc
     3481 cttgaacggg tactaaatca ccctgacgaa acgcaagccc gacgcttaat gacgctggaa
     3541 gatatcgtca gtggttattc caacgtgttg atttccctgg cagatagcca cggcaaaacg
     3601 gtgtatcact cccccggtgc gcctgatatc cgcgagtttg cacgtgatgc catacccgat
     3661 aaagacgcgc ggggtggcga ggtgtatctc ctttccggcc cgacgattat gatgccaggc
     3721 cacggtcacg ggcatatgga acacagcaac tggcggatga ttaacttgcc ggttggcccg
     3781 ttggtggacg gcaaaccgat ttatacgctc tacatcgcgc tgtcgatcga ttttcatctt
     3841 cattacataa atgatttgat gaataaactt attatgaccg catcggtaat cagcatcctg
     3901 atcgtcttta tcgtgctgtt ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc
     3961 cgtcaaatcc agaatatcac ctcgaaagat ctcgacgttc gccttgaccc gcagaccgtg
     4021 ccgattgagc tggaacagct ggtactgtcg ttcaaccata tgatcgagcg tattgaggat
     4081 gtctttactc gccagtccaa tttctcagcg gatatcgccc acgaaattcg cacaccaatt
     4141 actaatctca ttacgcaaac ggaaatcgcc ctcagccagt cgcgcagcca gaaggagctg
     4201 gaagatgtgc tctactctaa tctcgaagag ctgacgcgaa tggcgaaaat ggtcagcgat
     4261 atgctgtttc tcgctcaggc cgataacaac cagctaatcc ccgaaaagaa aatgctcaac
     4321 ctggcggatg aagtcggcaa agtgttcgat tttttcgagg cgctagcgga agatcgcggc
     4381 gtggagttgc ggtttgttgg cgacaaacgt caggttgcgg gcgatccgtt gatgctgcgt
     4441 cgggcgctaa gcaacctgct ttctaatgcc cttcgttata cgccaaccag agagacaatt
     4501 gtagtacgct gtcagacggt cgatcaccag gtgcaagttt ccgtcgaaaa ccccggtacg
     4561 cccattgcgc ccgagcactt accgcgattg tttgaccgtt tctatcgcgt tgacccctcc
     4621 cgccagcgaa aaggtgaagg tagcggtatt gggctggcga tagtgaaatc gattgttgtc
     4681 gcgcataaag gcacggttgc ggtaacgtca gatgcgcggg ggacaaggtt tgttatcaca
     4741 ttacccgctt aattattcag caaacggcaa cttttataac cagtgtaaaa ataacgtgcc
     4801 gcaatagtca tacatattaa tggtaaaaag ctgtcacaat tcataaaaaa ccttaatata
     4861 cgccacccta aacataacca gcgttaatgt aaggtttttg tgtggactgg cttcttgatg
     4921 tttttgctac ctggctctac ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt
     4981 tcatcagcgg gctggacgat ttttttattg atgtcgtcta ctgggtacgc cgcattaaac
     5041 gcaagttgag tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg tataaaccag
     5101 atgaaaaacc gttagcgatt atggttccgg cgtggaatga aacgggcgtc atcggcaata
     5161 tggccgagct ggcggcgacc acgctcgact acgaaaacta tcatatcttt gttggcacct
     5221 accccaacga ccccgatact caacgtgatg ttgacgaagt gtgcgctcgc tttccgaacg
     5281 tgcataaggt agtctgcgcg cgtcctggtc ccaccagcaa agccgactgc ctgaacaacg
     5341 tgctggacgc catcacccag tttgaacgta gcgccaattt cgcttttgct ggttttattc
     5401 tgcatgacgc cgaagatgtg atttcaccga tggaattgcg tctgttcaac tatctggtcg
     5461 agcgtaaaga tctgattcag atcccagtgt atccgtttga acgcgaatgg acgcacttca
     5521 ccagcatgac ttacattgat gagttttcag aactgcatgg caaagatgtt ccggtgcgtg
     5581 aagccctcgc cggacaagtg cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg
     5641 tgaccgcact gttagctgac ggtaacggta ttgctttcga cgtgcagagt cttactgaag
     5701 attacgacat tggcttccgc ctgaaagaaa aaggtatgac ggaaattttt gtccgttttc
     5761 cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt tttacagcac gcacggacgt
     5821 caaacatgat ctgcgtgcgc gaatatttcc ccgatacctt ttcgactgcg gttcgacaaa
     5881 aatctcgctg gatcatcggc attgttttcc agggctttaa aacccataaa tggacctcca
     5941 gcctgacgct gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca
     6001 gcttcctcgc gatgctggtg atgatccagc ttttgctgtt gctggcgtat gaaagtttgt
     6061 ggcccgatgc ctagcatttc ctttctattt ttagcggcag cgcatggtta atgaccctgc
     6121 tgtggctaaa ctttggcttg atggttaacc gcatcgtgca gcgagtgatt ttcgtcactg
     6181 gctactacgg cctcacgcag ggcctgcttt ccgtcctgcg tcttttctgg ggcaacctga
     6241 ttaactttat ggctaactgg cgcgtgctga aacaggttct tcagcacggc gatccgcgtc
     6301 gcgtcgcgtg ggataaaaca acgcatgact tccccagcgt cactggcgat acccgctcgt
     6361 tgcgcccgtt aggtcaaatt ctgctggaaa atcaggtcat cactgaagaa caactcgata
     6421 cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc
     6481 tgattagcgc cgagcagctg gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat
     6541 ccatcgatgc ctggcagatc ccgtcctcgc tgattgccga aatgccagcc tccgtagcgc
     6601 tgcattatgc agtactgccg ctgcgtctgg aaaatgatga gttaattgtc ggcagtgaag
     6661 atggtattga cccggtttcg ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt
     6721 acgtcattgt tctgcgggga caaattgtca cagggttacg ccactggtat gcacgccgac
     6781 gcggtcacga tccgcgggca atgttgtaca atgcggttca gcatcagtgg ctcacggaac
     6841 agcagaccgg tgaaatctgg cggcaatatg tgccgcatca gttcctgttc gccgaaatac
     6901 tgaccacgct cggtcatatt aatcgttcag caattaacgt gttgttattg cgccatgaac
     6961 gcagttctct gccgctcggc aagtttttgg tcaccgaagg cgttatcagc caggaaacgt
     7021 tggatcgcgt cctgacaatt caacgcgaat tacaagtttc gatgcaatca ctattactca
     7081 aagcaggttt aaacacagaa caggttgcgc aactggagtc cgaaaatgaa ggagaataac
     7141 cttaatcgcg tcatcggatg gtctggttta ctgctgacgt ctttattgag taccagcgca
     7201 ctcgcagaca atatcggcac cagcgcagaa gagctggggc tgagcgatta tcgccatttt
     7261 gttatttatc cccgtctcga taaggcgctg aaggcacaga aaaataacga cgaagcaacc
     7321 gccatccgcg aatttgaata tatacaccaa caggtgccgg ataatattcc gctgacttta
     7381 taccttgcgg aagcctatcg tcattttggt catgatgacc gggcgcggct gttgcttgag
     7441 gatcaactga aacgtcaccc aggagatgcc cgacttgagc gcagtctggc ggctattcca
     7501 gttgaagtga aaagcgttac gactgttgaa gaactgcttg cccagcaaaa agcgggcgac
     7561 gctgcgccga ccctgcgttg tcgcagtgaa gtcgggcaga ataccctgcg gctggcacaa
     7621 ttacctgtcg ccagagcgca actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa
     7681 acgctgcgaa ccgatctgct gcaacgggca atctacctga aacaatgatc ccaggcagat
     7741 acgctataca atgaagcacg ccagcagaac acattaagcg cggcagaacg ccgtcagtgg
     7801 tttgacgtgc ttcttgccgg gcagctggac gatcggatcc tggcactgca atcacagggg
     7861 atcttcactg atcctcagtc atatattact tacgcgaccg cgctggctta tcgtggcgaa
     7921 aaagcacgcc tccagcatta tctcattgaa aataagccgc tgtttaccac gggcgcacaa
     7981 gagaaaagtt ggctctatct gttatctaaa tacagcgcca accccgttca ggcattggcg
     8041 aattatacgg tgcagtttgc cgataaccgc cagtatgttg ttggcgcgac gctaccggtg
     8101 ctgttaaaag aaggtcagta cgacgcagcg caaaaactgc tcgccaccct ccccgccaat
     8161 gaaatgcttg aggagcgtta tgctgtcagc gtggcgaccc gtaacaaggc tgaatctctg
     8221 cgtctggcac gattgctgta tcagcaagaa ccgacaaatc ttccccgcct ggatcaactg
     8281 acctggcaac tgatgcagaa cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc
     8341 tatcctttcc agggcgatgc gcgtgtcagc cagactttaa tggcgcgact ggcgtctctg
     8401 ctggaaagtc atccttacct agcaacgccg gcgaaggtgg cgattttatc gaaaccctta
     8461 ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg gtattgcaga taattgcccg
     8521 gcaatagttc gcttgctggg cgatatgtcg ccttcctacg atgctgccgc ctggaaccgt
     8581 ctggcaaagt gttatcggga cacgctaccc ggtgtggcgt tgtatgcatg gcttcaggcc
     8641 gaacaacgac aaccgagcgc ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt
     8701 gaggactacg ccaccgcact ggcggcctgg cagaaaatca gtcttcacga catgagtaat
     8761 gatgatctgc tcgccgccgc caacaccgct caggcagcgg gaaatggcgc ggctcgcgat
     8821 cgctggcttc aacaggcaga acaacgtgag ctgggaaaca atgctcaata ctggtggctg
     8881 catgcgcgac gttatatccc agctcagcca gaactcgcac tgaacgatct cacgcgctca
     8941 atcaatattg ccccttctgc caactcttac gttgcgcggg cgacaattta tcgccaacgt
     9001 cataatgttc cggccgcggt gagtgatttg cgcgccgcgc tggaactgga accaaataat
     9061 agcaacaccc aggcagcgct tggttacgcc ttgtgggata gcggtgatat cgcacagtcg
     9121 cgggaaatgc tcgaacaggc gcataaaggg cttccggacg atccggcact gatccgacaa
     9181 ctggcctacg tgaaccagcg tctggatgac atgcctgcga cgcagcacta cgcccggctc
     9241 gtgattgatg acattgataa tcaggcataa ccccactgac cccagagcaa aatcagcaac
     9301 gcttcaattt ccgccgtttg catgaggagg tcggtcgccg ctggacgttc agtttcgatt
     9361 cttccatcgg cttgcgttcc ggcgcaatga gtaccgctaa caataatgtc ggcggtgcag
     9421 cgcctgggaa aagctatcgt agctacggac aactggaagc cgagtaccgc atcggacgca
     9481 atatgctgct ggaaggcgac ctgctctcag tttatagccg cgtctttgcc gataccggag
     9541 aaaacggggt gatgatgccg gtgaaaaatc cgatgtccgg caccggtctg cgctggaagc
     9601 cgctgcgcga tcagatcttt ttcctcgccg tcgaacagca gttgccgctg aacgggcaaa
     9661 atggcgcatc cgataccatg ctgcgcgcca gcgcctcatt ctttaatggc ggcaaataca
     9721 gcgacgaatg gcacccgaac ggttcaggct ggtttgccca aaacctgtac ctcgatgcgg
     9781 cgcaatatat ccgccaggat attcaggcgt ggacggcaga ttatcgcgtc agctggcatc
     9841 agaaggtagc taacggacag actattgagc cttacgctca cgttcaggac aacggctatc
     9901 gtgataaagg cactcagggc gcgcagcttg gcggtgtcgg ggtccgctgg aatatttgga
     9961 ccggcgagac gcactacgac gcctggccgc acaaagtcag tctcggcgtc gagtatcaac
    10021 atacctttaa ggcgattaat caacgtaacg gagagcgcaa caacgcgttt ctcaccattg
    10081 gagtgcactg gtaaatgcgt aagttcattt tcgtattgct gacactgctt ttggtcagcc
    10141 ctttttcctt tgcgatgaaa ggtattatct ggcaaccaca aaaccgagat agtcaggtta
    10201 ccgataccca gtggcagggg ctgatgagtc agttacgttt tcaaggcttc gataccctgg
    10261 ttttgcaatg gacccgttac ggcgatgcat ttacccagcc agaacagcgc acgttattgt
    10321 ttaagcgtgc cgcagctgcg caacagactg gcctgaagct gattgtcggg ctgaacgccg
    10381 atccggaatt ttttatgcac cagaaacagt cgtctgcagc gctggaaagc tatcttaatc
    10441 gcctgctggc tgccgatctc cagcaagcca gattatggag cgccgcgcct ggcataacgc
    10501 cggatggctg gtacatcagc gcggaaattg acgacctgaa ctggcgcagc gaagccgccc
    10561 gtcaaccttt gctaacatgg ttaaacaacg cgcagcggct gattagcgat gtttcagcaa
    10621 aaccggttta tatcagtagt tttttcgccg gaaacatgtc gcccgatggc tatcgccaac
    10681 tgctggaaca agttaaagca accggagtta atgtctgggt acaggatggc agcggcgtgg
    10741 ataaacttac cgctgaacag cgtgaacgtt atctacaggc cagcgccgat tgccaaagct
    10801 ccgccccagc aagcggcatt gtttatgaac tgtttgtcgc cggcaaaggc aaaaccttta
    10861 cagcgaaacc gaaaccggac gcagaaattg cctcgctgtt agcgaaacgt tcctcttgcg
    10921 gtaaagacat gctctatttc tctctgcgct atttgcccgt cgcgcacggc attctcgagt
    10981 attaaatccc ctccaggtaa gtcgggtacg acctggctta cctctttcgc tctcgtcaga
    11041 attccatcaa gattaattgc taaaaagcat gctaaagaaa gaagatcgtt atcggcgaac
    11101 gggccgatca ctcatgcagg aagcatagat cctttgagcg gagtttctga tatctgagga
    11161 gtgcgaaatg caattgagca gcagtgaacc ttgcgtggtg atcctgaccg aaaaagaggt
    11221 agaggtaagc gtcaataacc atgctacgtt tacccttccg aaaaactacc tggccgcctt
    11281 cgcgtgcaac aataacgtca ttgaactctc aacgttaaat cacgtattaa tcacccacat
    11341 caaccgtaac atcatcaacg attatctgtt gtttttaaat aagaaattaa cctgtgtaaa
    11401 gccctggtcg cggctggcaa ccccggttat cgcttgtcat agccgtacac cggaagtgtt
    11461 ccggctagcc accaaccaca gcaagcagca atccagcaaa ccctgcgaag cggagttgac
    11521 gcgcgcatta ctttttaccg tattgtctaa ctttcttgag caatcgcggt ttattgccct
    11581 actgatgtat atcttacgca gcagcgtccg cgacagcgtc tgccgcatta ttcaaagcga
    11641 tattcagcat tactggaatc tgcgaattgt cgccagttcg ctatgtttaa gccccagcct
    11701 gctcaaaaag aaattaaaaa acgaaaatac cagctatagc cagattgtca cagagtgtcg
    11761 tatgcgttac gccgtacaga tgttattgat ggataacaaa aatatcactc aggtggcgca
    11821 attatgtggc tatagcagca cgtcgtactt tatctctgtt tttaaggcgt tttacagcct
    11881 gacaccgttg aattatctcg ccaaacagcg agaaaaagtg atgtggtgaa gggcaaagcg
    11941 gaaacggata agacgggcat aaatgaagaa gaaatggcgc gccctgcagg attcgaacct
    12001 gcggcccacg acttagaagg tcgttgctct atccaactga gctaagggcg cgttgatacc
    12061 gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt gctgagtcaa tggcttttga
    12121 tctggttgct gaacaagtga acgaccgcgt ctgattttct gatttatttc gctatagcgg
    12181 caaacaaacg cacaccgctg cgcgtctgaa tcaagaaaac ccgtattttc atgtatcaaa
    12241 gtacaattta ccgccctaac ggaaaattgt ccgctcctat gagactggta actatgaaac
    12301 caacgtcggt gatcattatg gatactcatc ctatcatcag aatgtctatt gaagttctgt
    12361 tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga tgattaccgc ataaccatcg
    12421 attatctccg aacccgtcct gttgatttaa tcattatgga tatagacttg cccggaacag
    12481 acggtttta
//
LOCUS       DAJTUN010000114        12263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTUN010000114 DAJTUN010000000
VERSION     DAJTUN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.114"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1189
                     /gene="edd"
                     /locus_tag="OO470_003128"
     CDS             <1..1189
                     /gene="edd"
                     /locus_tag="OO470_003128"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6252298.1"
                     /translation="ESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSP
                     LRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAM
                     ARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGLLHE
                     DVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGN
                     LGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGM
                     PELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGD
                     IIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQGATC
                     ITF"
     gene            1226..1867
                     /gene="kdgA"
                     /locus_tag="OO470_003129"
                     /gene_synonym="eda"
     CDS             1226..1867
                     /gene="kdgA"
                     /locus_tag="OO470_003129"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6252299.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1923..3101)
                     /gene="purT"
                     /locus_tag="OO470_003130"
     CDS             complement(1923..3101)
                     /gene="purT"
                     /locus_tag="OO470_003130"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6252300.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3235..3525
                     /gene="yebG"
                     /locus_tag="OO470_003131"
                     /pseudo
     CDS             3235..3525
                     /gene="yebG"
                     /locus_tag="OO470_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3592..3948
                     /locus_tag="OO470_003132"
     CDS             3592..3948
                     /locus_tag="OO470_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6252301.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4275..4934
                     /locus_tag="OO470_003133"
     CDS             4275..4934
                     /locus_tag="OO470_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6252302.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5144..7204
                     /gene="ptrB"
                     /locus_tag="OO470_003134"
     CDS             5144..7204
                     /gene="ptrB"
                     /locus_tag="OO470_003134"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6252303.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7201..7863)
                     /gene="exoX"
                     /locus_tag="OO470_003135"
     CDS             complement(7201..7863)
                     /gene="exoX"
                     /locus_tag="OO470_003135"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6252304.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7887..8543)
                     /locus_tag="OO470_003136"
                     /pseudo
     CDS             complement(7887..8543)
                     /locus_tag="OO470_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8645..8875)
                     /gene="holE"
                     /locus_tag="OO470_003137"
     CDS             complement(8645..8875)
                     /gene="holE"
                     /locus_tag="OO470_003137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6252305.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9014..9388
                     /gene="yobA"
                     /locus_tag="OO470_003138"
     CDS             9014..9388
                     /gene="yobA"
                     /locus_tag="OO470_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6252306.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9392..10264
                     /gene="copD"
                     /locus_tag="OO470_003139"
                     /pseudo
     CDS             9392..10264
                     /gene="copD"
                     /locus_tag="OO470_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10277..10618
                     /locus_tag="OO470_003140"
     CDS             10277..10618
                     /locus_tag="OO470_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6252307.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11011..12087)
                     /locus_tag="OO470_003141"
                     /pseudo
     CDS             complement(11011..12087)
                     /locus_tag="OO470_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12053..>12263)
                     /locus_tag="OO470_003142"
     CDS             complement(12053..>12263)
                     /locus_tag="OO470_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6252308.1"
                     /translation="RRWARNGNIYPAPELHGRSYRVVPEAFYINPNKVDTDITHHQPN
                     GRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3043 a         3003 c         3102 g         3115 t
ORIGIN      
        1 ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg gtactgccaa
       61 caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt cttttgttca
      121 tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg ttacacgcat
      181 gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga aagtggtggt
      241 gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca tgcacctggt
      301 ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg acctttctga
      361 tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta accacttcca
      421 ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc tgctgcatga
      481 agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac catggctgaa
      541 taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca atgtgatcgc
      601 ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg gtaacctggg
      661 ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg aagcgccagc
      721 ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt tgctggaccg
      781 cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc cagaattaca
      841 taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg cgttagttac
      901 cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg taacaccaga
      961 agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc gtgtgaatgg
     1021 acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc gcgaaccgca
     1081 cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca gcgccttgcg
     1141 tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag acgacaaatt
     1201 tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag aatcaatcct
     1261 gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg cggtgccgat
     1321 ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc gtaccgagtg
     1381 tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg tgggtgccgg
     1441 tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac agttcgcaat
     1501 tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta ttcctctgat
     1561 tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt tgaaagagtt
     1621 caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga tcgcgggtcc
     1681 gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact accgtgacta
     1741 cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg cagatgcgct
     1801 ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag aaggcgctaa
     1861 gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt tttttgccgg
     1921 gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat ggcgtcaaca
     1981 acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat ttccggctta
     2041 ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc aaacgtgaca
     2101 ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata ctgacggatc
     2161 ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga gagatcttga
     2221 gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact gaaaatcacc
     2281 tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag tgccagcacc
     2341 actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg ctgtggttgc
     2401 caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca gaaatggacg
     2461 ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac gacgccttca
     2521 acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca tgcctgagca
     2581 agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat caccggtttt
     2641 acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt gctatcggca
     2701 aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg gataccctcg
     2761 cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc ttcctcttca
     2821 agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata atgtggtttt
     2881 tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac atgggagcga
     2941 tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac ctctacgccg
     3001 agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa taacatcacg
     3061 cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat cccctgatta
     3121 attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga tgttgatttc
     3181 tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct gcaaatggcg
     3241 gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt taccagcaaa
     3301 aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga cacctagctg
     3361 acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct atggctggca
     3421 gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc cccacaggtc
     3481 gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa cttgcgctcc
     3541 tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa catgaaaaaa
     3601 agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt cgctgccaat
     3661 aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc tgccggaatt
     3721 gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc agatgatcaa
     3781 aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat tcagggtaaa
     3841 gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat tcattaccag
     3901 gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg gcttctgata
     3961 attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga acgcaccaca
     4021 atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca aacaatgcca
     4081 ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata cagtaaatac
     4141 cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt gcgcatcttt
     4201 tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact aaaaatggtt
     4261 attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt gggcaaagca
     4321 gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg tcaggcgcat
     4381 taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt cttacgaaat
     4441 atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg ctgtggaata
     4501 aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc gcgcaaagta
     4561 cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc gctaaaagtg
     4621 atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt gaagccggcg
     4681 tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg gatccacaac
     4741 gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc ctgagctgcg
     4801 cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg ggtgacgcgc
     4861 tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag caaaaacgca
     4921 ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg ggctatccag
     4981 aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc gatatccgcg
     5041 ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc ccggtgagtt
     5101 ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac caaaagccgc
     5161 ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt actactggct
     5221 gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag aaaatagtta
     5281 cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa aggaaatcat
     5341 cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct accgctatcg
     5401 gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg cattcagcga
     5461 agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc atagtgagtt
     5521 ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc tggcagaaga
     5581 ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag gtaactggta
     5641 cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact cctggacttt
     5701 ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc gtcacgccat
     5761 cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata cctattatgt
     5821 cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca gcgccaccac
     5881 cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg tttttctgcc
     5941 gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt atctgcgttc
     6001 caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg agcaacagtg
     6061 ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc tgtttaccga
     6121 ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa ttaaccgcaa
     6181 gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct ggattgccta
     6241 caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga ccacaccaga
     6301 cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac aaacagaagt
     6361 gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg cccgtgatgg
     6421 cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag gacacaaccc
     6481 gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt tcagttttag
     6541 ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc gcggcggtgg
     6601 tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa atacgtttaa
     6661 tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc cttcgctctg
     6721 ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta atcaacgccc
     6781 ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa caacgatgct
     6841 tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc cgcaggatcc
     6901 gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg cacaggctta
     6961 tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt gggaaccggc
     7021 aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat tgctctgtac
     7081 cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg aaggcgtagc
     7141 gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta cgcctgcgga
     7201 ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca tgctgttcag
     7261 gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg aaaccgcttt
     7321 gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg tgatatcggc
     7381 catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg cggcggtgat
     7441 ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta cattgagctt
     7501 gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac gacgggccag
     7561 tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt caaagctggc
     7621 gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt caatccacgg
     7681 tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg gactaatagg
     7741 acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa tgacatcaac
     7801 agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga taatgcgcaa
     7861 catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc ttgccaaccc
     7921 tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac gatgagacga
     7981 ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac ggcaatcgag
     8041 tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg ttctcccaga
     8101 cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg cggttgttca
     8161 tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact ctgatggtag
     8221 ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa gcgatcgtta
     8281 tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc ataacaaagc
     8341 gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata atcacacccc
     8401 agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc ggccctcaca
     8461 aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc atattgcgct
     8521 gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca aattatgggg
     8581 gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta actctgtata
     8641 agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa gccaaacggt
     8701 gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca cgttcaaccg
     8761 cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc gccgccaaat
     8821 cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc agcattctta
     8881 atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc aagccggaat
     8941 tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt tattacaaaa
     9001 aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg caatcctgac
     9061 cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc agtatcctgc
     9121 ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct cggaaggcgt
     9181 tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata ttaaaacatt
     9241 accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg ccgattcgct
     9301 gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc ataaaacgaa
     9361 aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga tcgcgctgcg
     9421 atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg gtgcctggct
     9481 ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac aacagcacgc
     9541 tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg ggttgatggg
     9601 aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc aaacgcagtt
     9661 tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg tcgcccttat
     9721 gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat ttattctgct
     9781 ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc atcagactaa
     9841 tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc cggtgctctg
     9901 gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc tgatgcgttt
     9961 ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc ttaatgcatt
    10021 gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct tattgctcaa
    10081 agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg ttcttgtacc
    10141 gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga ctaaactcga
    10201 atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc tcgaaccttt
    10261 ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac tcaccagttc
    10321 tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag tgggtaaaga
    10381 caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta atgctttgta
    10441 tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac aaaaggaagt
    10501 tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg atcctaacaa
    10561 tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac tctgttaatt
    10621 acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc accagaacgg
    10681 gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt ttgcacgcaa
    10741 actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag cctgcattaa
    10801 tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg aatacaggaa
    10861 tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg tctccccgaa
    10921 attccccgaa atttactcga atttctgtat tccggtcttt tttggttata tcacaaccaa
    10981 aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt tttctctctc
    11041 atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct gagcaaattc
    11101 ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt gaaaagttgg
    11161 tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt tctttgtcag
    11221 gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac tgattagata
    11281 atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta aaccagcgac
    11341 aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt gcctgatgtg
    11401 gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc gacgttgtcc
    11461 tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat ctgctgcctc
    11521 cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta ttttcggtgc
    11581 tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt cgcggaacat
    11641 atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt caatctacaa
    11701 atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc ctcctaattt
    11761 ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg acgcttttct
    11821 ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg agtcgacccc
    11881 tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat gatttgcttc
    11941 aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt cttctttcgt
    12001 cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt ttctaggcaa
    12061 attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga ctgtctctcc
    12121 cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt gggttgatat
    12181 agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca ggataaatgt
    12241 ttccattcct tgcccatcgt ctc
//
LOCUS       DAJTUN010000115        11799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTUN010000115 DAJTUN010000000
VERSION     DAJTUN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.115"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            155..955
                     /locus_tag="OO470_003143"
     CDS             155..955
                     /locus_tag="OO470_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6252309.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1033..1803
                     /locus_tag="OO470_003144"
     CDS             1033..1803
                     /locus_tag="OO470_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6252310.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(1851..3209)
                     /gene="mltD"
                     /locus_tag="OO470_003145"
     CDS             complement(1851..3209)
                     /gene="mltD"
                     /locus_tag="OO470_003145"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6252311.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(3281..4036)
                     /gene="gloB"
                     /locus_tag="OO470_003146"
     CDS             complement(3281..4036)
                     /gene="gloB"
                     /locus_tag="OO470_003146"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6252312.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4070..4792
                     /locus_tag="OO470_003147"
     CDS             4070..4792
                     /locus_tag="OO470_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6252313.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(4789..5256)
                     /gene="rnhA"
                     /locus_tag="OO470_003148"
     CDS             complement(4789..5256)
                     /gene="rnhA"
                     /locus_tag="OO470_003148"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6252314.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            5321..6052
                     /gene="dnaQ"
                     /locus_tag="OO470_003149"
     CDS             5321..6052
                     /gene="dnaQ"
                     /locus_tag="OO470_003149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6252315.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6185..6261
                     /locus_tag="OO470_003150"
     tRNA            6185..6261
                     /locus_tag="OO470_003150"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6219..6221,aa:Asp,seq:gtc)
     gene            complement(6392..7438)
                     /locus_tag="OO470_003151"
     CDS             complement(6392..7438)
                     /locus_tag="OO470_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6252316.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(7449..8434)
                     /gene="tssG"
                     /locus_tag="OO470_003152"
                     /pseudo
     CDS             complement(7449..8434)
                     /gene="tssG"
                     /locus_tag="OO470_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8431..10314)
                     /gene="tssF"
                     /locus_tag="OO470_003153"
     CDS             complement(8431..10314)
                     /gene="tssF"
                     /locus_tag="OO470_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6252317.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10330..10824)
                     /gene="tssE"
                     /locus_tag="OO470_003154"
     CDS             complement(10330..10824)
                     /gene="tssE"
                     /locus_tag="OO470_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6252318.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(10821..11651)
                     /locus_tag="OO470_003155"
                     /pseudo
     CDS             complement(10821..11651)
                     /locus_tag="OO470_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(11638..>11799)
                     /locus_tag="OO470_003156"
     CDS             complement(11638..>11799)
                     /locus_tag="OO470_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TagK domain-containing protein"
                     /protein_id="HCR6252319.1"
                     /translation="PDILHLLSPEYAPETAHNTVLPDLTRKEHRIIGIDSHYRINPAQ
                     HGEIYHDKQ"
BASE COUNT         2678 a         3106 c         3202 g         2813 t
ORIGIN      
        1 tgttctcaat aatgagcaaa tttctgacag gtgtaagcac ttgcttacat aacaatatac
       61 aattgctcgt tgaaagagtg agctaaaatc cctataacag tagaaccctc ccgagtgcgg
      121 aagggttgac gtaatagagg tttcaaagtc aaaagtgcga aaaaacacct atgccatgcg
      181 ctatgttgcc ggacaacctg cggaaaggat cttaccgccg gggtcttttg cgagcatcgg
      241 ccaggcatta ccacctggag aaccgttaag taccgaagag cgtattcgga tcctggtgtg
      301 gaacatatac aaacagcaac gcgctgaatg gttgtcggta ttaaagaact acggcaaaga
      361 tgcacatctg gtgttattgc aggaagcgca gacaacgcca gagttagtac agtttgcgac
      421 cgctaactat cttgccgccg atcaggtgcc cgctttcgtg ctgccacaac atccttccgg
      481 tgtaatgacc ctttcggcgg cgcatccagt gtattgctgc ccgttacgtg aacgagaacc
      541 cattttgcgt ctggcgaagt cggcactggt gacggtctat ccattacctg acacccgcct
      601 gttgatggtg gttaatatac acgccgtcaa cttcagtctg ggcgtggatg tctatagtaa
      661 gcagttactt cctattggtg atcagatagc tcaccacagc ggcccggtca ttatggcggg
      721 agatttcaat gcctggagcc gtagaaggat gaacgcgtta tatcgctttg cgcgggaaat
      781 gtcgctgcgc caggtgcgtt ttaccgatga tcagcgccgt cgggcgtttg gccgcccgct
      841 cgattttgtt ttctaccgtg gtctgaacgt cagcgaagct tctgtattgg ttacgcgcgc
      901 ttccgatcac aatccgctac tcgttgaatt cagtcccggc aagcctgata aataaggtat
      961 gtcaggtctg ccacagggca gaccaacgtt tggcgctgtc cttttatact caaacaacaa
     1021 aaggacagcg caatgacaac acaatcccac catgaccacg tagaaaagca gtttagctct
     1081 caagcctgcg aatatctgac cagtaccgta catgcatccg gacgagattt acagcgcctg
     1141 gcggtgcgtc tggctgatta tcctggtgca agtgcacttg atatgggctg cggagcaggg
     1201 catgccagct ttgttgctgc gcaaaacgtg agcgcggtgg tggcgtatga cttatctgcc
     1261 caaatgctgg atgtcgtggc acaagctgcc gaagcccggc aactgaaaaa tatcaccacc
     1321 cgccagggat atgccgaaag tctgccattt gccgataacg catttgatat tgttatcagc
     1381 cgttattctg cccatcactg gcatgatgtt ggtgcagcac tgcgtgaagt gaaccggata
     1441 ttgaaacctg gcggtaggat gattgtgatg gacgtaatgt ctccgggtca tccagtgcgc
     1501 gacatctggt tacagacggt agaagcatta cgcgatacct ctcacgtacg aaactacgcc
     1561 agcggtgagt ggttgaggtt aatcaatgaa gccaatctga tagttgataa tttaattaca
     1621 gataagttac cgctggaatt ttgttcatgg gtcgcgagaa tgcgtacgcc agaagcgtta
     1681 gtagacgcta ttcgcattta ccaacagagc gcatcgacag aggtgagaac gtattttgcc
     1741 ttgcagaatg acggctcttt caccagtgat atcatcatgg tagaagcaca taaagcggca
     1801 taaataaaaa aggcaccggg ggaatcggta cctttttatt atctggtttg tcaggaatct
     1861 ggcatgttgt tgtttttcac aaacaacgtc agcttgtcgc ctggttgcag attcgcagtg
     1921 tcgctgttcc agcgcatcac atctttgatg ttcacgccgt ggcgtttagc aatgcttgaa
     1981 agcgaatcgc ctttgcgcac acgatacgta atgctatcgc tgttgtttgc cagtcgctgt
     2041 gcgctactgc ctgcaccaat cgtcaaactt tggcctggct tcagcttaga tccgcgcagt
     2101 ttgttccact gctgcaaatc tttggtgctt acgccgagac gtgaagcgat gcttgaaagc
     2161 gtgtcgccag agcgtacggt gtaaacacgg ctgttaagcg gcgtattgtt ggcaaccagc
     2221 gtcgactgta cagcagcaat ttcgcctgaa gcgagagatt cacgcagttg atctgcatgc
     2281 ttctttggca ccatcacgta ctgcggccca cttgcgccca gcgtggagcc tttcacgcca
     2341 gcgttgaatg tcttcagctt gctgacggaa atccccgcca tatctgcaac cttcgccatt
     2401 tcaaccgggc tactcaggtg cacacgcgcc agagcacggc tttcatcggt cgttggcaga
     2461 cgtacgccat aacgcttgct gtttttgaga atatcactca atgccagcat tttaggcacg
     2521 tactgcttcg tttcctgcgg caacggtaac gaccagaagt ccgtggattt cccacgcgct
     2581 ttgttcgttt taattgcctt catgacccga ccttcgccgc tgttataagc cgctacggtc
     2641 agaagccagt cgtcgtcaaa cattttgttc agacgctgca tcatattcag cgcggcagtt
     2701 gttgaagcaa caacatcgcg acgcgcgtca taattgcggg tctgtttcaa accataattg
     2761 cgccccgtgc tcggaatgat ctgccagatg cctgcggcat tggcgccaga cgttgcgtga
     2821 ggatcaaaag cgctctccac tatgggtagt agtaccagtt ccataggcat gttacgtttt
     2881 ttaacttgcc ctgctatcca gtacatatac ggctctgccc gtaaagttac atcgtggaga
     2941 tagctcttat tgcgtaaata tttctgtttc tgttcgcgaa tccggtcatt ttccggaatt
     3001 cccatcttta gctcgtcgcc aatgaaagcc cacaagtcac catctggcgc gatagacgtc
     3061 ccatcgtcca tccatcgtgc ctgacttgta aactttgctg cttccccttg accagctgca
     3121 gaaaggctct gtgcgtgctg ttgaacgttg ccggtactct ggcaacccac gagcaggaca
     3181 gaggcgagta atatcgcttt tgccttcatg tgtgtgtcaa tagttgctta aaagacgacc
     3241 gatcataacg gcgaacggag ccgatgacaa gaaagtttta tcagaaccta tctttctttg
     3301 accttaacca tgcaaaacgc tcttcaggtt gttgcaataa tgtttcttca ttaattccat
     3361 taattaaatc aatatcttcc gttcttaaaa aaacattaat ttgccgctca tttttcagaa
     3421 ttacgggtag tgttatttga ttttttgccc gtaactcctt aactttacga taataatcat
     3481 ttatggacaa atcgtgcgga aaaatactca aagcaaactt catatttgat aacgtatatt
     3541 catgagcaca acataccaat gtatcgtcag gtaacgcact tagcttttta agtgattgat
     3601 acatttgtaa agctgtccct tcaaacaacc gaccacaccc accagaaaac agcgtgtcgc
     3661 cgcaaaatag ataaggttta ctgaagtaac agatatgtcc taaagtgtga cccggtgtgg
     3721 taattacact aaattcatgc cctaaaacga aggcagtttc gccatctttg actacctgtg
     3781 ttgttccctt atcttgtgtc tcttgtggac catacaccac aatttgcgga aacttttcca
     3841 ccagttcttt tacgccgcca acgtgatcgt ggtgatggtg ggttagaaat atggcctccg
     3901 gttgccagtt attggcggta atggcgttta atactggctc tgcgtctccg ggatcgacaa
     3961 tcaggcagcg acctgcctca tcattcaaaa cccagatgta attgtcatca aaggcgggaa
     4021 tactgttaag attcatagat tacctctcag tgtaaaacgg aaggttgtga tgaaaccggc
     4081 aagagtccct caaactgtcg tggctcctga ttgctggggc gatttgccct ggggagagct
     4141 ttatcgcaag gcgctggagc gccagctcaa cccgtggttc actaaaatgt atggttttca
     4201 tctgcttaag attggcaatt taagcgcaga aatcaattgc gaagcgtgcg cggtttctca
     4261 tcaagtgaat gtttctgcgc aaggaatgcc cgtccaggtg caggcggatc cacttcatct
     4321 tccttttgcc gataaatccg ttgatatttg tctactggca cataccttgc cgtggtgcac
     4381 cgatccgcat cgtttattac gtgaagccga tcgggtactg attgatgatg gctggctggt
     4441 gattagtggc ttcaacccca tcagtttaat gggattacgc aaacttgtgc cggtattgcg
     4501 caaaacctcg ccctataata gccggatgtt tactctgatg cggcaactgg actggctctc
     4561 tttgttgaat tttgaagtgc tgcacgccag ccgtttccac gttctcccgt ggaacaaaca
     4621 cggaggaaaa ctattgaatg cgcatattcc tgcgcttggt tgcttacaac ttattgttgc
     4681 ccggaaacgg actattcctt taacgctaaa tccgatgaaa cagagtaaaa acaagccacg
     4741 aattcgccag gcggttggag ccacccggca atgtcgtaaa ccacaggctt aaacttcgac
     4801 ttggtagccc gtatcttcca gtgtgggatt catcgccgcg gcacgagcca gttcatcaca
     4861 gcgttcgttt tccgggtgtc cggcatggcc tttaacccat tcccatttga tttgatgctg
     4921 ccccaatgca gcatcaagac gttgccagag atcgacattt tttactggtt ttttgtctgc
     4981 tgttttccag ccacgttttt tccagttatg gatccactgg gtgataccct ggcggacata
     5041 ctggctgtcg gtactcaaaa tgacttcgca atgttctttt aacgcctcca gcgcgacaat
     5101 agcggccatc aactccatac ggttgttggt ggtgcgggtg tagcctgcgc taaaggtttt
     5161 ctcgcgtccg cgatagcgta aaatagcgcc gtaaccccca ggtccaggat tacccagaca
     5221 cgaaccatcg gtgaaaattt ctacctgttt aagcatctct ggtagacttc ctgtaattga
     5281 atcgaactgt aaaacgacaa gtctgacata aatgaccgct atgagcactg caattacacg
     5341 ccagatcgtt ctcgataccg aaaccaccgg tatgaaccag attggtgcac actatgaagg
     5401 ccacaagatc attgagattg gtgctgttga agtagtgaac cgtcgcctga cgggcaataa
     5461 cttccatgtt tatctcaaac ccgatcggct ggtggatccg gaagcctttg gcgtacatgg
     5521 tattgctgat gaatttttgc tcgataagcc cacgtttgcc gaagtagccg atgagtttat
     5581 ggactatatc cgcggcgcgg agttggtgat ccataacgca gtgttcgata taggctttat
     5641 ggactacgag ttttcgttgc ttaagcgcga tattccgaag accaatacct tctgtaaggt
     5701 caccgatagc cttgcggtgg cgaggaaaat gtttcccggt aaacgcaaca gcctcgatgc
     5761 gctatgtgcc cgctacgaaa tagataacag taaacgaacg ctgcacgggg cattactcga
     5821 tgcccagatc cttgcggaag tttatctggc gatgaccggt ggtcaaacgt cgatggcttt
     5881 tgcgatggaa ggagagacac agcagcaaca aggtgaagca acaattcagc gcattgtacg
     5941 tcaggcaagt aagttacgcg ttgtttttgc gacagatgaa gagctggcag cacatgaagc
     6001 ccgtctcgat ctggtgcaga agaaaggcgg aagctgcctc tggcgggcat aaatacatgt
     6061 aaaaggcgct aaaaatagcg acttgggcga tttttgcagc aaacgattca aaagatgaga
     6121 aaaatcgttg acgaaggtcg aggcaatccg taatattcgc ctcgttccca acggaacaca
     6181 acgcggagcg gtagttcagt cggttagaat acctgcctgt cacgcagggg gtcgcgggtt
     6241 cgagtcccgt ccgttccgcc aatattcaga aagcccgaat cagtaatgat tcgggctttc
     6301 gtcgttttgg tgaagtgaga atttttcact ccgaagtaaa acggatatga tttaaaaaat
     6361 ggccagacat tataaattct ggccacttct gttactccag cgcatcccat ttctccagca
     6421 gttctactgg aatttcattt attacctccg agacgcgttt tccgaccagg cgttctgcct
     6481 ggcgcagcaa aggaatggtc gggctgctcg gttcgctgtg ctcaaaccag cgacggatga
     6541 cacgcaggcg ttccagcgcg tcgtttcggt cgcggatctg ccggggttct gcgccggtca
     6601 tcggtgatat ttcctccgcg atatgcaccg gtagcgcaat ctgctctgcc tgcatctgtt
     6661 cagtatgaac gatggtggcg ttttccggct gctcagcctg tggttgtggc aggatctccg
     6721 gcgtggtgct ctgcactgct cccggtaaca gggccagcag ttgcgtcaga cgggagaagt
     6781 ccggtgcctg gtcattcagc gtttcacgcg catggcgttg cagccgctca gtggtctctg
     6841 ctgcctggcg aaaggcatcc agcggcagtg tgccgcgcgc ttccaggtct gccagttgct
     6901 ggcgtaccga ttctggtgcc agcgcatccg ccgggcgtaa tgcagatagc gaacgctcca
     6961 catcccgcac ctgaaggcgc atggcagcat tatttgacag tgtaatgcca cgaatatccg
     7021 ccattacacc ttcgtggtcc agcaacgccg ccagcgcgtt actgcgtgcc accgcagcat
     7081 cttcggcggt gatatcttca gtcgcgagca gttgaggatg gatggcgtcc gggtacgtgg
     7141 agcacaacgt ttccagcagc gtgagcgcct ccgacagtcc cctggcaccg gcctgctgga
     7201 tacggcagcg cagcaggaca accagaatgc ggatatcttt gctgcgcatc agcagacgat
     7261 gggcgtcccg ctcaagctcc gcccagttaa tgttttccgg cgtactgaca aaatcgccat
     7321 attgcgcttc ttcacgcggt gcggcgcggg tgaacagcag caggtattcc ggatcgtact
     7381 ccagatccgg gccgcagggg cattccgccg aaattgggtc agacaggaat atttccattt
     7441 aattatcctc agtgcgccag gtactgttct ggttcgaaaa tcatgcccgt caccgggttg
     7501 tcgtgcatgg ctttacccgc ccatgcagtc caacccagtt ggtacggacc accggtaaca
     7561 gccggagggg cggcgtgggg ctttagctgt agctcaattt cccagcagta ttcataacca
     7621 ctaaaggcgc ggaccagttc tgtcaggacg ggcaggttat tgctgccagg cagaaaacgt
     7681 cggtaatcct ccagcgtcag cgggccgata accagccgga acttatactg catgtccggc
     7741 acggcctggc cgatgagtgc accattgcca ataacggaag agggggcagg catccccagc
     7801 cgggtgatct catcgtccgc cacggctatc cagtgcagga catactcctg tatccggaaa
     7861 ggtacaccga aatagtgcgc cagcgtggtg gccagaccgt ccggattgcg ggactcacgc
     7921 accagatggg ccgatgccgc cagacgagcg tgatctggca acgggctgtc agcgctctcc
     7981 cgcaaatcct gcccgctgag gctggcgaca taaaaggcaa agcggtcatg ttgcggttta
     8041 tccagaccgc ccccagcgga ctgggcgctg cgccatgcct ggtaaaactg cgtcagccag
     8101 cggtgatgaa aaagatcaga aaaacgggtc agcgtcggct ttcagtccgg ttacgcgcca
     8161 gttcggtgta gtgcagcggc agtggaccgt tcggccccca cagcccgagg ctgtacagac
     8221 tgatgtgcag gcgcccttcc tgccagccca cactggcgat ttctcgtggc gcaaaggtca
     8281 gcgcgggcgt ttgtccgagg cgaaattttt ccatccgtgg ttgccaggtg ttgcccgccg
     8341 gaacggtaca caactgcaca tccacgcggc gcatcaggtt cagaaaaccg taacgccagg
     8401 ggggggcctg cgcctgagtg aaatcgttgc tcatacgctt ccccgctgtc ccgccctgac
     8461 aggccaggtc ataattttgc cgcgctgcat gctgtgcagc gtcatctgcg agaaggtatt
     8521 aatggaaaca tggcgggcga tatagtgttc cagcaccagg ccgaacagat acggactgat
     8581 gccggaaaaa ccttcttcat ccaccgtcag ttcacaactg acgccacgcc catagaccag
     8641 tagtccggaa ccgggcagac ggcgggtgac cggggtcgtt ttacaaccaa tcaggctgcg
     8701 cacctggcgc gattgcggac tgtcatgagc aggaataaac aggttcagca gatctcgcaa
     8761 cgcctggccg ccggtacggt gatccagatc ggccagcggc agataattaa aagataactg
     8821 gcggatcaac cgccatgcca tctcacgttc agccagcggg ggctgcggcg agcgtggcgg
     8881 gcggataagc cctacgcccg ccaccgggat ggctgcatcc acggttagat cgtcccggcc
     8941 attgcgggca ataagacagg gcagatcgcg gttagtgacc atggccgtga tggtgatgtg
     9001 gcgcagattt tccgggtacg gcgcttcata ctggtcaacc agcgacagga acacttccga
     9061 gcccgtatag ggggtacggg tgccgtaacg gcgggcgttt tctgataagc ggcggggctc
     9121 ccggcgcagg gaaaaataac gtccgtgatt gccttcatca ttattgcggg tgtggtagag
     9181 cgggcgaaag gccatcttcc gtgtggtgtc ggtttccagc ccttccactt cctggaccga
     9241 gaacacctca taatccagcg gatgggttct gtccaccacc agatgctgtt ccgtcgtgct
     9301 gtgcgtgaca tcgatccggg cggtgacgcg gggaaacagg ttgataaccg gggtacagaa
     9361 caggctgaac tgtgccgcat ttgtctggtg gatcagccag tccggcggca ggcggttcag
     9421 caggataaca atctccgcca cgccaccgtc aattttctgt aatccggcag acaggcctgt
     9481 cggggtgaaa aaatagaagc gttccggaca ggcaaaatat tcatgcagca ggttatgacc
     9541 gtgaaacacg ttccacgcca gcggcagcag cccctgatcc ggctccaggc cttcgtacat
     9601 aacgggctgt tgcagattca catccagcgc gccgtcaaaa tgccccggaa tgccagccag
     9661 ggtcgcgatg gcgctggtat gtagaagttc taacaggtgt gaggccgtac gctcctcgcc
     9721 gcagagataa aacggcagcc tgtccagtcc ggcaagctga ctgaacgtta gttcaccaaa
     9781 ggtgcgcagg gtaatacgca gcgccccggc gacgtgaatg ttggccggca ggtagcggtg
     9841 cagggcaggc atatccggtg gcgcggcggt cagtcgcgcc tcttcgatgg cgagcggcca
     9901 cagcgttaca tcctggctgc tgcgaaactg gcaggcagtg ctttcgcctt ccggaatggc
     9961 ggagaaaaag gctgtgtcgc gcgggacggt aaatcctttc gccagatcgc cttcttccgt
    10021 atcgggatgc aactgcgcca cgctcattga gggcgtgggt gtgacgtaat tggggctgac
    10081 cacttccagc aggcgctggg tgaaacgggg gaattcagcg tcaattttta gctgcgtgcg
    10141 ggcggtcagg aagctgaagg cctcgaccat tcgctctaca tacggatcgg caatatccgt
    10201 tccctgcatg cccagccggg cggcgacttt gggatgacgg gcggcaaatt cggctcccgt
    10261 ttcccgcaga tagctcagtt cgcggttgta gtattccagt agccgtggat ccatcggtct
    10321 ctctctgaat taaaaaaacg taatgcgact ctgctccata tccagcgcgc tacgtacccg
    10381 gaatgcagtc ggatacggct gcgtgaggat ctcgccgcga atttcaaact gtaatgtgtt
    10441 atggccactc tggcggcgct catcctgaag tgggatgatt tgcagagtgg acgctttcag
    10501 ccggggttca aaccgggtga tggttcgcaa aatggccctg cggatatcgt cccacttgtg
    10561 ctcatacaga aagctgcccg ccagtggcgg caggccgtag ttaagtacgg acgctgccgc
    10621 gtacggataa cgtgcagcat caatatcatc ttcatggctg acggtgttga gcagaaatga
    10681 caaatcccgg cggatgatct ccttaatctg aaccggactg acgctgatac cgtggtcgcg
    10741 tttctggttt ggggcgtcgt cacacaggcg gtcaaacagc gtgggcagca ggtgattgcc
    10801 tggacgctgg cgggaagcgc tcacgcgtcg cttccttcag cggtgttaaa ggtacaggtg
    10861 gacaaatcca gcaggctgat atcgccgtga ccggtgagcc acaccctctg acccagcgcc
    10921 tgtaccagtg tctctcccgg accgtcctgc cagaccgttt cccggcacag acgcagggag
    10981 tcggcagcct tttcagagcc gctgtagcgg ggaaacagct aggcactgtg tgtatcgcca
    11041 ttgttcaggg tgacgttgac gggtttccag atcaaatcga tcagatgcgc aggccgcggc
    11101 gatttcagcg aacagatttg tgaaaacggc agccagatat atgctccgcc cgcgatcagt
    11161 tcgagtaccg gaccggtacg ggagtcgctg tcgctaatcc agtcaaatgc gccgccattc
    11221 cactgcccgg aagtgtctgt tatggcctca agcgcgcggc tgcggtgagc atcagcttcc
    11281 tgcgcttcac cacgggcgtt acaggcgagg gcggtcagca gatcctccat ccaggcgggc
    11341 ggcgggagaa taacaccggg ccgctgttcg ccctgaaagc aggcgtggcg gtatatctca
    11401 cagcggatca gctcgccaaa tacctgcgct tcgcgagtat aatttgcgtc catccgggcg
    11461 catagctgga tctgttgcag tgcccgcgac cagtcgcctg ccacgcacaa taactgaaac
    11521 aggctgtggc ggcgggaggc ctgcgctggg tgcatcctga cctgttgctc tgccatctga
    11581 atgccctcca ccacggaata atcccgcagc agcgccgaca aggtaatcgg tagctcgtta
    11641 ttgtttgtca tgatatatct ctccatgttg tgccgggttt atccggtaat ggctgtcaat
    11701 gccaatgatg cgatgctctt tccgtgtcag atctggcagt acggtgttat gcgctgtttc
    11761 cggggcgtat tccggggaaa gtaaatgcag gatatcagg
//
LOCUS       DAJTUN010000116        11416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTUN010000116 DAJTUN010000000
VERSION     DAJTUN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.116"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(16..441)
                     /gene="arsC"
                     /locus_tag="OO470_003157"
     CDS             complement(16..441)
                     /gene="arsC"
                     /locus_tag="OO470_003157"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6252320.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(454..1743)
                     /gene="arsB"
                     /locus_tag="OO470_003158"
     CDS             complement(454..1743)
                     /gene="arsB"
                     /locus_tag="OO470_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6252321.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1797..2150)
                     /gene="arsR"
                     /locus_tag="OO470_003159"
     CDS             complement(1797..2150)
                     /gene="arsR"
                     /locus_tag="OO470_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6252322.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2893..2976
                     /locus_tag="OO470_003160"
     CDS             2893..2976
                     /locus_tag="OO470_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6252323.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3030..4382)
                     /gene="gorA"
                     /locus_tag="OO470_003161"
     CDS             complement(3030..4382)
                     /gene="gorA"
                     /locus_tag="OO470_003161"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6252324.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4454..5296)
                     /gene="rlmJ"
                     /locus_tag="OO470_003162"
     CDS             complement(4454..5296)
                     /gene="rlmJ"
                     /locus_tag="OO470_003162"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6252325.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5499..7541
                     /gene="prlC"
                     /locus_tag="OO470_003163"
     CDS             5499..7541
                     /gene="prlC"
                     /locus_tag="OO470_003163"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6252326.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7549..8301
                     /gene="rsmJ"
                     /locus_tag="OO470_003164"
     CDS             7549..8301
                     /gene="rsmJ"
                     /locus_tag="OO470_003164"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6252327.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8350..9819)
                     /gene="dtpB"
                     /locus_tag="OO470_003165"
                     /pseudo
     CDS             complement(8350..9819)
                     /gene="dtpB"
                     /locus_tag="OO470_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10137..10571)
                     /gene="uspA"
                     /locus_tag="OO470_003166"
     CDS             complement(10137..10571)
                     /gene="uspA"
                     /locus_tag="OO470_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6252328.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            10965..11300
                     /gene="uspB"
                     /locus_tag="OO470_003167"
     CDS             10965..11300
                     /gene="uspB"
                     /locus_tag="OO470_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6252329.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
BASE COUNT         2833 a         2932 c         3037 g         2614 t
ORIGIN      
        1 gtggatatcg ccgctttatt tcaggcgctt acccgcttca tcaaccactt tctcgccatc
       61 ttccttggag aatgcgcctt tttgcgcatc tggcagaatt tccagcacca cttctgaagg
      121 gcggcacagg cgagttccca gcggcgtcac cacaatcggg cgattaatca gaatcgggtg
      181 ctgaagcata aagtcgatta accgatcgtc agtaaattta tcttccgcaa ggcccagctc
      241 ctcatacggt tcgacgtttt tacgcagcag cgcgcgtacg gaaatcccca tatcggcaat
      301 gagtttgacc agttcatcgc gcgttggcgg agtttccaga taatggataa tggtcggttc
      361 tgtgccgctg ttgcggatca tctccagcgt attacgcgac gtgccgcagg ccgggttgtg
      421 ataaatggta atgttgctca tatcagtatc tcattacaaa gtgaaagaga gacgtagcgc
      481 cagcgcagcc agcgtcacaa acagcacagg cagggtcatg ataatccctg tacggaagta
      541 atatccccag ctgatagtca tattcttctg cgaaagtacg tgcagccaga gcagcgttgc
      601 caggctacca atcggggtaa ttttcggccc caaatcgcag ccaatcacgt tggcataaac
      661 catcgcttct ttgataacgc cagatgccgt gctgccatca atggacaacg cgccaaccag
      721 taccgtcggc atattgttca taatagaaga gaggaaggcg gtgaggaatc cggtgccgag
      781 cgtcgcggcc cacaggccgt tatccgccag cacgttgagt acgccagaaa gatattccgt
      841 taatccggca ttgcgcaggc cataaaccac cagatacatg ccgagcgaga agatgacaat
      901 ctgccagggg gcaccgcgca ggactttacc cgtattaatc gcatgaccgc gtttagcgac
      961 gacaaataat atcagtgcgc ccacagctgc aatggcgctc accggaatgc cgagcggttc
     1021 caagacgaaa aatcccacca gcagaagcag taaaacaacc cagccagttt tgaacgtagc
     1081 aggatctttg atcgcttctg cgggagattt cagcagcgcc atatcgtagt tctgcggaat
     1141 atctttgcga aaatagagat gtagcatcac cagcgtggca acaatcgcgg cgatatccac
     1201 cggcaccatc accgaggcgt attcgcgaaa gccgaggcca aagaaatcag cggaaacgat
     1261 attcaccagg ttggagacaa taagcggcag gctggcggta tcggcaatga atccggccgc
     1321 catcacgaac gccagcgtcg tgcctttact gaatcctaaa gccagcagca tggcgatgac
     1381 aatcggcgtc aaaataagcg ccgcgccatc attggcaaac agggccgcaa cggcggcacc
     1441 gagcaggaca atccaggtaa acagcaagcg accgcgtcca ttgccccagc gtgaaacgtg
     1501 cagcgctgcc cattcaaaaa agccggactc atccagcagc aggctgatga taatgacggc
     1561 aataaacgcc gccgtcgcgt tccagacgat attccacacc accggaatat cacccggatg
     1621 aaccacgccc gtaaccaacg ccagtaccgc gccgagcgtt gcactccagc cgatgcctaa
     1681 acctttcggc tgccagataa ccaatacgat ggtcaggaca aagatagcgc ctgccagtaa
     1741 cataatgcct cccggataaa acacatgtgc aaatccatat gtgtttagct aaatttttaa
     1801 ctgcaaatgt tcttactgtc tccggaacag ttttgtctgg ccaggttgcg gacaattgcc
     1861 tgaatctttt cctgttcaca tcgccaggct tcatcaataa ttttcgccgc ccatgctgga
     1921 atatgcggcg ataagcggta atgaacccac ttaccttgct tgcggtccag caatagcccg
     1981 ctttcacgca gcaatgccag atggcgggat atcttgggct gcgactgatc gagggcagtg
     2041 cagagatcgc agacgcataa ctctcccagt tcgctaagca gtaaaacgat gcccagacgg
     2101 gtttcatcag caagaatttt gaacaattgg atgggtaaca gaaatgacat attgcgctcc
     2161 tgattgttgc aggtagtgtc tctcttcgaa gcggataagt caaaaacata tatgacttaa
     2221 cgaatgtgta agtgcagagg aaggtaatag gtgtgaattt tgagttggct attcatttga
     2281 aaggcggttt gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt actcttcagc
     2341 ctgagttggt ggctctggct ggagtgagaa tgcccacatc taacggttca atactattag
     2401 ccagtggcta acattgcaag cgttagcgat ttcttcagct ggagaaggga cagtttgcta
     2461 tacatggtgg tgaaagagga tttatatact ttcatcgggt cgctgccgta ccaagccttg
     2521 tccgaatccg gacgtaaaaa aaccgacttc gcgtcggttt ttttactctt cagcccagag
     2581 ttggtggctc tggctggagt gagaatgcca tcatctaacg catcaatgct aataccatac
     2641 tgaaactatt gcaaggttat gctggttatt tagcctaatt acactgtatg attatccagt
     2701 tagctctgag gcattttcac tctggcaatg cgcataaacg ctttcaaagt cctggtcaga
     2761 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agattgccca catctaacaa
     2821 tgaggcatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     2881 tggagaaaac gggtgattga taaagcaatc atcgttcttg gggcgttaat tgcgctgctg
     2941 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3001 agagcacact actcttagcc ctttaacatt taacgcatgg tcacgaactc ttctgccgcc
     3061 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3121 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3181 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     3241 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     3301 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     3361 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     3421 agacggcgac ccgctgcaac tgccaccggt gtcagttcca ctgcacccgt gttatcgccc
     3481 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     3541 tcgttggttt taacgccagc ggcttcgagg ttgatgttgt cattggcagg ctcgcgacca
     3601 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     3661 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtatgcaa ctgtgggcct
     3721 tcggcgttca tcacttcgac cagcgtttca gagatcatcg ggtcgaagct gcgcagcggc
     3781 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gccagccagc
     3841 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     3901 aagaagccat cggaatcaat accgtattcc acgcctggaa tatccgggtg gctcggacga
     3961 ccgccggtgg cgatcagaat atgatcggcc gtgatggttt cgccgttaac ttccagcgtt
     4021 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4081 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4141 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4201 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     4261 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     4321 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     4381 atgacatatt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     4441 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtacctgccg
     4501 gaaccagttt gctgtgcagc cacgacagca cgttattcat ctgctgttcc agcttccacg
     4561 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagca
     4621 ccgccagttc aatttgcaga attttgcgaa tgccggtcgc ttccaggtcg tggatcatgc
     4681 gcttaatttg ctggcgcagc accaccggat accacaatgc gtaagtgcca gtggcgaaac
     4741 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     4801 gcgggtcgat aaggattaaa ccacggcgag aaaccggcgg cagtttggcc ttaagttgct
     4861 ggaaaccgtc ggctttttcg acgcgtgcac ggctgtcttt ctgaaatcca gaacgcagca
     4921 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     4981 ggcgagcaat caacggcgaa ccggggtagt aacgcaactg gccgctacgg ttgaagtgtt
     5041 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5101 cgataccttc gagatattcg ccggtacgct cggcatgttc gctacctaac tgataacgcc
     5161 cggcccctgc gtgggtgtcg agatagagaa acggtttatc tttctctttc agcgactcga
     5221 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     5281 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaattggccc gtattcgggg
     5341 cgcacaaaag cctatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     5401 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     5461 tgagcgcttc cttaacctct ttaaccagga ctgcgctaat gacgaatccg ttactgactc
     5521 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgacta
     5581 aggcattgaa cgactgccgc gaaaatgtgg agcgcgtagt agcgcaaggg gcaccgtaca
     5641 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgttttgggg cgtatcttct
     5701 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcgtacgaac
     5761 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     5821 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     5881 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     5941 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6001 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6061 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6121 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6181 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     6241 cgaatgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     6301 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     6361 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     6421 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     6481 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     6541 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa agcggttaac ggcctgtttg
     6601 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagatgtt gatgtctggc
     6661 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     6721 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat ggctgcgtag
     6781 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     6841 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     6901 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     6961 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7021 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7081 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7141 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7201 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     7261 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     7321 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     7381 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     7441 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     7501 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     7561 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     7621 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     7681 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     7741 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     7801 aaaggcgatt atttgccgga tgtggtggac gccaccgcag ggctggggcg cgatgccttt
     7861 gtactggctt cagtcggctg ccgcgtgcgg atgctggagc gtaatccagt ggttgccgcg
     7921 ctactcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     7981 gagcggttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8041 ccgcaggtgg tctatctcga tccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8101 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattactg
     8161 gagcctgcac gcttgctggc gaccaaacgc gtggtggtaa agcgcccgga ctacgcgcca
     8221 ccgctggcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     8281 tatgcaggta cgccagtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     8341 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     8401 cagcatcact actgcaacgc ccagcgtgac cagaccaatc ttaccaaaca cgttggtata
     8461 gacgggcaac gtctcaagcg gatcggtaat gttgtccggc accgcggtaa atgttgccac
     8521 atagccgccc agcaagaacg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     8581 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     8641 ttcacccagg ctctagaata aatacaccag cacgataaac catggcgatg tcagcccctg
     8701 gtcatccgca aaccacattc ccgcagcagc agccgtcaaa aagcccagtg agcacataaa
     8761 catgctgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     8821 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     8881 gctgaccggg ttgatggaaa aaccgagaat ttcatgatgc acgttgttga tggcaaagaa
     8941 gttcagcgat gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9001 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9061 gaagatgata gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9121 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9181 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
     9241 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
     9301 tgaataaccg aatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
     9361 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
     9421 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
     9481 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
     9541 aatcgccagc acaagtgctc caagaacaat ggtgcgtttg gtccccaaca ggtggtcgcc
     9601 gacatagccg ccaatggaaa tgaggccata gaccagtgca gcaaaagcac caaaagtgac
     9661 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
     9721 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
     9781 aggttgctgc agcatcccca tgggtgttgt tgtattaata tttattaacc ttccaatacc
     9841 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
     9901 ttcttaatat tgatgcggta aatagattaa tacactatta caacagaaaa taaccagctg
     9961 atcacactaa tgatggaaaa tatgatggtg ttaaattttg tattggcagg agattttgat
    10021 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10081 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtggag ggatgattat
    10141 tcttcttcgt cgcgtagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10201 cgtgcggaag acatcagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    10261 atatcgtatt tcttgattgc atcgaccaga acctggccta ggtcgccgct gccgctcagg
    10321 gtttcagtga ttggatagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    10381 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    10441 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    10501 actgctttct ctaccagaac tttgctttcc ggggagagat cgactgcgat gagaatgtgt
    10561 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    10621 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    10681 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    10741 tgcttacccg ttagataaac aaattttcga catgaatatt atattgatag tggttaacct
    10801 tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg atgttggtca
    10861 ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat cgggagcggt
    10921 agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata agcaccgtcg
    10981 cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac
    11041 tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa tatgttgatg
    11101 gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc gtttggtata
    11161 tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc tgtgagcggg
    11221 tgcgtcggca gtttattctg actagcgcat tgtgtggtct ggtggtggtc agcctgattg
    11281 cattgatgat ttggcattaa gcacttcagc aataataacg tataaaaaaa gcgggccagt
    11341 aaactgaccc gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga accagtacag
    11401 cccgccggaa agtagt
//
LOCUS       DAJTUN010000117        11219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTUN010000117 DAJTUN010000000
VERSION     DAJTUN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.117"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..442)
                     /locus_tag="OO470_003168"
     CDS             complement(<1..442)
                     /locus_tag="OO470_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6252330.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFA"
     gene            complement(473..1165)
                     /gene="fimC"
                     /locus_tag="OO470_003169"
     CDS             complement(473..1165)
                     /gene="fimC"
                     /locus_tag="OO470_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6252331.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1385..1927)
                     /gene="fimA"
                     /locus_tag="OO470_003170"
     CDS             complement(1385..1927)
                     /gene="fimA"
                     /locus_tag="OO470_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6252332.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2408..3274
                     /gene="folD"
                     /locus_tag="OO470_003171"
     CDS             2408..3274
                     /gene="folD"
                     /locus_tag="OO470_003171"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6252333.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3276..3488
                     /gene="ybcJ"
                     /locus_tag="OO470_003172"
     CDS             3276..3488
                     /gene="ybcJ"
                     /locus_tag="OO470_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6252334.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3596..4105
                     /locus_tag="OO470_003173"
     CDS             3596..4105
                     /locus_tag="OO470_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6252335.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4141..5526)
                     /gene="cysS"
                     /locus_tag="OO470_003174"
     CDS             complement(4141..5526)
                     /gene="cysS"
                     /locus_tag="OO470_003174"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6252336.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSICAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5700..6194
                     /gene="ppiB"
                     /locus_tag="OO470_003175"
     CDS             5700..6194
                     /gene="ppiB"
                     /locus_tag="OO470_003175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6252337.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6197..6919
                     /gene="lpxH"
                     /locus_tag="OO470_003176"
     CDS             6197..6919
                     /gene="lpxH"
                     /locus_tag="OO470_003176"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6252338.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7037..7546
                     /gene="purE"
                     /locus_tag="OO470_003177"
     CDS             7037..7546
                     /gene="purE"
                     /locus_tag="OO470_003177"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6252339.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7543..8610
                     /gene="purK"
                     /locus_tag="OO470_003178"
     CDS             7543..8610
                     /gene="purK"
                     /locus_tag="OO470_003178"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6252340.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8747..9640)
                     /locus_tag="OO470_003179"
     CDS             complement(8747..9640)
                     /locus_tag="OO470_003179"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6252341.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9637..10284)
                     /locus_tag="OO470_003180"
                     /pseudo
     CDS             complement(9637..10284)
                     /locus_tag="OO470_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10287..>11219
                     /gene="allC"
                     /locus_tag="OO470_003181"
     CDS             10287..>11219
                     /gene="allC"
                     /locus_tag="OO470_003181"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6252342.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDE"
BASE COUNT         2701 a         2923 c         2874 g         2721 t
ORIGIN      
        1 ctgcaaaatc aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat
       61 tgttttgctg ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga
      121 gtacctggtt aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg
      181 attcaaatac gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat
      241 acacgcccgc aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc
      301 cattctctaa taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta
      361 aaatggcaag agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat
      421 ccgtagtagt gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta
      481 agttcacttc tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct
      541 ttccctgcac tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat
      601 tccccaactt ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat
      661 tatgaacatt gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg
      721 gtaattcttg taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt
      781 gcaaaacatt cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca
      841 tccagaacag agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag
      901 tattttcgct tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt
      961 ttacaccgct gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat
     1021 ttttaatcca taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta
     1081 atgcgatccc tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca
     1141 tcaataactt tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa
     1201 taaggagtat aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt
     1261 aataaagcgg catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt
     1321 cagacaaccg gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt
     1381 ttgattattc gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac
     1441 ttgtaccggt gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt
     1501 tctggttggt tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta
     1561 tttcaatacc gacacccgtt gctgttgttg tatttgtact ggatgcaatg gccaataaat
     1621 tatcattgat tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag
     1681 gatcgcagtc gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat
     1741 tgaaaatatc ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca
     1801 ctgaacaggc agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg
     1861 cagcataact acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta
     1921 atttcatata tattccttca atctcattta tcgactccac atccgtatat aaccgattac
     1981 tttatttaag acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg
     2041 aaaatatgca ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa
     2101 atatattcaa cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta
     2161 tattgtcagg tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag
     2221 atttacaacg ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg
     2281 tgcgcctaac cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc
     2341 gcgccccgct tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct
     2401 ctctctgatg gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga
     2461 agttgctcaa aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt
     2521 tgtgctggtg ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg
     2581 tgaagaagtc gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga
     2641 gctgctggag cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca
     2701 actgccgtta ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa
     2761 agacgtggac ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct
     2821 gcgtccctgc accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt
     2881 cggcctcaac gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga
     2941 actgttgctc gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca
     3001 tcacgtagaa aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg
     3061 tgactggatc aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg
     3121 caaagttgtg ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc
     3181 tgttcccggc ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc
     3241 gtgcgttgaa tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac
     3301 atccgcacgt tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc
     3361 aggcgaaaat cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca
     3421 aacgctgcaa aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg
     3481 ttgcctgatc cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc
     3541 tctacgcccc gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc
     3601 aaccgttatt acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg
     3661 tctgttattt gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa
     3721 atttggcgtt gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt
     3781 tgcgtttgtt gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct
     3841 gattcgctgc tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt
     3901 aaccactggc ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc
     3961 tccctggcag gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg
     4021 gcatcaggtg attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg
     4081 aatgttgtgg tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa
     4141 ttacttacga cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt
     4201 aagacggtca cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg
     4261 ctgttgaatt aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg
     4321 caggaacgct tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg
     4381 agatgccatt gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc
     4441 catatcaaac agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat
     4501 aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag
     4561 cgcagtgtag aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt
     4621 cagctggctg cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta
     4681 tttcagcaca tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc
     4741 aaccatcacc attccggagt gcatccagta gttcacatac tgaccatcat gggcacagat
     4801 ggactgcgcg atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat
     4861 atcaaagtgg ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg
     4921 acgacccgcg ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag
     4981 aacgaagtcc atcgggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag
     5041 ctggtcgaga tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat
     5101 cacgtcgccg ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc
     5161 aataatttct gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa
     5221 agcatcaaaa tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc
     5281 gccattttca ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata
     5341 cttcagttta tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt
     5401 acgcccgtga ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac
     5461 ttccccggcg tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt
     5521 tagcatcgaa gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat
     5581 ttcccgcgcg aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac
     5641 caccctatat atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa
     5701 tggttacttt ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac
     5761 ctgaaacagt taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt
     5821 tccaccgtgt tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac
     5881 aaaaagccac caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg
     5941 gtacgctggc aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca
     6001 acgtggttga taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact
     6061 gcgtgtttgc tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa
     6121 ccggtcgtaa cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga
     6181 ccgttagcga gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga
     6241 accggcgatc accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc
     6301 gctgtatatt cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact
     6361 ccatcgccag atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt
     6421 cattcatggc aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac
     6481 attattgccg gaagaaaagg tgctcgaact ttatggtcgc cgggtgttaa ttatgcatgg
     6541 cgacacgctg tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc
     6601 ctggctgcaa atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg
     6661 aatgcgcgcg aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa
     6721 ccaaaacgct gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca
     6781 tacccatcgc ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt
     6841 actgggtgcc tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct
     6901 gattcatttt ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt
     6961 ttccttgctc tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac
     7021 aggagtttta agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg
     7081 gtccaaaagc gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt
     7141 cccgcaccac gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc
     7201 cgaaagcgcc gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca
     7261 cctgccaggc atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag
     7321 cgccgcactg agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc
     7381 ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca
     7441 aattctcgcg actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca
     7501 gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc
     7561 ctcggtaacg ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct
     7621 gtctggccgg tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt
     7681 accgctgaga tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg
     7741 gcctttgtga accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt
     7801 ttcgataagc tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg
     7861 cctgcggtgt ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat
     7921 gacggtcgcg gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt
     7981 tacggtgaat gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc
     8041 gcgcgcggct ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac
     8101 ggtattttgc gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc
     8161 gaagagatgc tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag
     8221 tgttttgtca ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc
     8281 ggtcactgga cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt
     8341 accgatctgc cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt
     8401 ggtagcgatg tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac
     8461 aaagaagtcc gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg
     8521 cgtctgactg cgacgctgga agccttaatc ccgctgctgc cgccagagta tgcctgcggc
     8581 gtgatttggg cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc
     8641 gtaaacgcct tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca
     8701 agcgtcgcat caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac
     8761 aggtccccgc ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg
     8821 gtttactacg gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat
     8881 cggctttggc aaatggagct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg
     8941 gcgttcccca gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat
     9001 taatctgctc ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg
     9061 ccccatcttc cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt
     9121 tgagcaacaa ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca
     9181 cccggcgcaa atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct
     9241 cttgttcttc tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat
     9301 catcaggcga aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg
     9361 cgctcaagct ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat
     9421 ccagcggata cggttctacc tctttccacg ccagattctg taacgccagc agccccacct
     9481 gcggcccgtt gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg
     9541 gtacagcact ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga
     9601 gtaaggcgtt gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg
     9661 ttggccaagc cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag
     9721 cgaatcaatc gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag
     9781 tggcgaggcg aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt
     9841 aacgagttga agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc
     9901 gccataatac caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa
     9961 tccggggcct ttacccagcc agtaccgcca gtcggtctta acgccattga gcgcggcctg
    10021 aaaacagtga cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag
    10081 tggcccgaaa agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg
    10141 atgcgaaggc ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg
    10201 cccgaggcga atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca
    10261 taacgcatcg aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc
    10321 tgccctggct ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt
    10381 cgccggaatg gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg
    10441 aaacacgttt cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac
    10501 aggaagtggt tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg
    10561 ggcaattcgg cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg
    10621 caccgttacg tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt
    10681 atgtcttctg gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata
    10741 tctgtgatgc caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc
    10801 cgaacgcccc actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac
    10861 agggctgtgt gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc
    10921 agcgtcgcta tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg
    10981 gttatcgtcg tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag
    11041 cgaaaaagat gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata
    11101 cggtaaatgt ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg
    11161 ccgtgctgcg cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaa
//
LOCUS       DAJTUN010000118        11190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTUN010000118 DAJTUN010000000
VERSION     DAJTUN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.118"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1950)
                     /locus_tag="OO470_003182"
     CDS             complement(<1..1950)
                     /locus_tag="OO470_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6252343.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSG"
     gene            complement(1884..2507)
                     /locus_tag="OO470_003183"
     CDS             complement(1884..2507)
                     /locus_tag="OO470_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6252344.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2504..4192)
                     /locus_tag="OO470_003184"
                     /pseudo
     CDS             complement(2504..4192)
                     /locus_tag="OO470_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4341..6968)
                     /gene="gyrA"
                     /locus_tag="OO470_003185"
     CDS             complement(4341..6968)
                     /gene="gyrA"
                     /locus_tag="OO470_003185"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6252345.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7115..7837
                     /locus_tag="OO470_003186"
     CDS             7115..7837
                     /locus_tag="OO470_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6252346.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(7965..11190)
                     /gene="yfaL"
                     /locus_tag="OO470_003187"
                     /pseudo
     CDS             complement(7965..11190)
                     /gene="yfaL"
                     /locus_tag="OO470_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2572 a         3123 c         2779 g         2716 t
ORIGIN      
        1 accgctgacg gcatgactta acccagcgag aattaagccg tctttatcgc gtaatgtcag
       61 attgtagttg ccaggtttag cgaaattgac ggtaaaggat ttgccgcctg acggtagctc
      121 tccgctatgg ctcgtgcggt cttcgagacg caaccattca tacgtaacag gaacctgttt
      181 tgaagattcc agcgcggcat aacggaacac aaccgactcg ccgctattac tgtattgtgc
      241 ggcggtactt aaagagtaat gcgccaggcc gcgttcaatg aggatctctt tggtggtggt
      301 gacgcgatac gccgcgccgt cactggcgga gacggttaac aaatagcggc tcggtttatc
      361 ggcggcgggg agatttaacg ccacatgacc gctggcgtcg gacaccgttt cgctgccttc
      421 cagcgacacg gggaaacgtc cggcataacg caaatcgtta ccgaccattg ataattgctg
      481 agcgcgcaaa cttaactgca cgcgggcatt ttttaccggc tcgccatccg ggtagagcag
      541 ttgcagtttg ccgctgaccg cttcgccagt tttgaactct tttttgtcga gagctaaacc
      601 aatctcgaaa tgtggcttga tgtagtttgc cacgcgaaaa ctgctgctat agacctgatt
      661 gcggtaagca agacgtaact cataacctcc ggcaacggca ttttctggca ggcggaaact
      721 tccctgcccg ccattgcgcg catccagcgt gacatcgacg gtttgcaaca gactgccgtt
      781 agcgtccagc accgaaagct tcgccggggc gctgacgatg ggggatgaat gcaacggatc
      841 gcggaactcg cggcccatca ctttaacatc gacacgatcg cctgcgcgat atagcgggcg
      901 atcggtaaaa atatacaagc gggtgttgta gatttcgctt tcgtagaaga agttctcgga
      961 gacaaaaacg ccgccttcag catccttacc cagaatgtat gaacgttctg gcgatatatg
     1021 ttgtaactgc aaagtaccgc tgtcatcggt caccccgcgg gtcatcacgc caagaccgtc
     1081 agtccacaag atctctgagc cgggcttcgc ttcaccctgt tttttacccg cggtccatac
     1141 cagaagctcg ttacctgaca ctttgctaag cgccacggta tcggaaacaa acaccaccgt
     1201 cgtcgcccga tacccaccta ccatcgcctc gacgaggtac agccccggct cttgttggcc
     1261 gagaggaata taaatattac ccggctgcgg cgagatgaaa ttgctggatg cgccttccag
     1321 ttttacccct tgctgcggct cgaccggttt agcctgccat agtggataac ggaactgttc
     1381 caccaaggga tattttttca gcggggaaaa ctggttgttc tgtacataac gggaaggttt
     1441 aataatggca ttgccgagat gtaattcggg taatgcctga gtcacattct gccgtgactg
     1501 agaagagaaa gtacgctgca tcacgcggcg agatttgccg taccagttat cccacagcca
     1561 ggtcagcgta ttgttcagcc cgtcgcccag atattgcggt tgcaccacaa tgcgatgcag
     1621 gtttttctgc tggcgcaaaa atgccatcgg gtcaggaata cgatataggc gaacgtccac
     1681 gccgccgtac tcttccatct gatagcgccg ataatcacgc cccggcgctt ccagtcgcac
     1741 tttcgcctct tcactgctgc taaaactgct gtcagcaagc aaaaagaatg ttcccccggc
     1801 gggcggcgca tagttactgg aaggaagcga atcatcagct ttagcaagcc ctgttcccac
     1861 aagagacagg gacaagcacg ctgctatcgc gccagaaaat ttaaacgata aatgccaatg
     1921 aaattgggat tggaatcgtt gggtatccat cgggtgtcct tccatgtcat aagttgttgc
     1981 agactgactg cgcgcattcc gttgtcagtt ttcgtggcgc ttccggtgtg gtagatgacg
     2041 taacgcccca tccagaccat taagtgctgg gcatcgccct gatcgaaaaa aatcatatcg
     2101 ccaggcagcg cctggtttat gtcttggcca ataaactggc tgttgtactg aatcaaatta
     2161 atcgcggtca cgtaggggcc gtttttcccg ctcccctgat tccagttttg cgccagttga
     2221 cgctgttcag gtgttagcgt catctctggc ggcaaatact ggctagataa accgttactt
     2281 tttagccatt tactgtcgtg aactttcagc gtctcgttcg ccgcaaatcg caccaggccc
     2341 gcacaatcct gctgatacca gcgtggactt ggcccctggc ggagctgttc ttgtgcaatg
     2401 cgtacaaacc aggcgcgaaa cagcctggat tgttcgacat tcagcatttc gctatgggca
     2461 acaacacaac acagccagca aatcagcgcc agcagcccgt gtctcatagc ggctgccagg
     2521 ttatgggtag ccactgccag gcgacaccgg gttccatctg ggccagcttc atgacataac
     2581 gcggttgttg agataaagcg tccagcttcg gcattaataa agtttgtgcg gcgttataaa
     2641 aaaccggttc gagattcttc ggcagactgg tcaacgtttc gttacgcagc agtttcgcca
     2701 cgccttgtgg attgatatag agcggaacga tgccatcagt gggtattaca tccaccattg
     2761 ccgggcgggt tttattcagt gtttgcaggg cgttattaac cagcgtgtca tcgagggaga
     2821 aaagcagcgt tttgttttgc atcgccagcg acacacggaa aaaataatcc gacattaatt
     2881 gatcgggttg cgccgcctgc gctttcggat actgaccgta tcgggaactc acttcgcgac
     2941 accagatttg cgcttcgccc tgctgagtct ggcttaccgg caaaacacct tctggcgctt
     3001 tgctttcgtg cgcaccaata ttttgcgtaa acagtttccc tggcaattgc gcctgttcgg
     3061 cagtgccatc aaactgaccg acaaacagcg gggtttgcaa ttttgagtct tcataccagc
     3121 acagccccgc ggcaccgtct aacgccccat tcagcttgtc gttttcctgg ctgatgtgcg
     3181 aaagcatctc ttcggcaata ccgtgtgaat acggcaccgc cacacagaag ctggctccgg
     3241 caggcatact gttccagacc ggcgtaaaat cgaaactggc atctacgctg gcggattcat
     3301 catttaacgc cacaaaactg tgccagccgt cgttacccat ttcgaagcgt acgccagcaa
     3361 aagaaggcat taaccgctgg tagccaaaac ccagcaacct ggcgctgacc acgatgcgca
     3421 ggcgtactgg cgttttttcg gctgtacgct cttccaggcc aaagctggct tgccagcgtt
     3481 ttttaccgct caacaaatca cttgcgatcg ccgtggcttc ggtatcctgc tgatcatctt
     3541 taaacaacat atccgtactg gaaaacacca gcatcttgtc ctgatatgtc gcgaacatca
     3601 gggcgttatt gccgttatag cgcaactgat aaacaggaat agtttcacta tttatcttga
     3661 tgctactgat ttccgttttg cttaactagc tgtcgctggt agcggcaaac aacaatggct
     3721 ccagcagttt gcttaaacca ctgcgctgga tcagcaccat gtaatgtgaa agatggcctt
     3781 gtttatcgtg ccatagcgcc gcctgcgcgg gctgatctaa gagtgacgaa aagagcttat
     3841 ctttcagcgt gagatcgtgt tcatagacaa tgcggcgaat gctgccttca atgcccagac
     3901 gatcggcatg attctgataa tagaaaacga aatcttcgct cagaacatcg tggagaaacg
     3961 gaatggtgag gagatctttg ggaagctggc tcagagagtc gctgtctaga aagaggtccg
     4021 gctcattgag atcgatttgc agattgttgt gcaccaccag cggcgacaac gttttttctg
     4081 gcccactgcc agcatattgc aacgcccaga cgccagcgga aagcagtgct attgcgccaa
     4141 aacctacaag accatagaac cgccagcctt tcgccttttt ttcaccactc attgccacat
     4201 tccttgtgta tagccagcca ttttttacgg gcacagccaa actttaccgc gccctaatac
     4261 gacaaaagcc cagactttgc agcctggact tttcaattca aacaagggag atagctccct
     4321 tttggcatga agaagtaaaa ttattcttct tctggctcgt cgtcaacgtc cacttccgga
     4381 gcaatttcat catccccttc cgcggcactg ccgtcgatgg tatccagatc ttcctcgtca
     4441 accggttcag caacacgctg cagacccact acgttttcat cttccgcagt acggatgagg
     4501 atcacgccct gggtgttacg gcccacgatg ctgatttctg aaacgcgagt acgtaccagc
     4561 gtaccggcat cggtgatcat catgatctgg tcgcagtcat ctacctgcac cgcgccaacc
     4621 actaaaccgt tacgttcggt aaccttgatg gagataaccc ctttcgtcgc acgcgacttg
     4681 gttgggtatt ccgccactgc agtacgttta ccgtaaccgt tttgcgttgc ggtgaggatt
     4741 gcgccatcgc cacgaggcac gatcagagag acgactttat cgccttcacc taagcgaata
     4801 ccgcgcacac cagtggtatt gcagcccatc gcacggacag aagactcttt aaagcgcacc
     4861 actttacctt cggcggagaa cagcattact tcatcttcgc cactggtcag atcaacgccg
     4921 atcagctcat cgccgtcaac cagtttgatc gccactttac cggcggtacg cagacggttg
     4981 aattcggtga ggacggtttt cttcacagta ccgttagcgg tcgccatgaa gactttcacg
     5041 ccttcttcaa actcggtcac cggcaggatc gcggtgatac gttcgtcctg ttccagcggt
     5101 agcaggttga cgatcggacg accgcgcgcg ccacgagtgg cttccggcaa ctggtaaact
     5161 ttcatcgaat agacgcgacc acggctggag aagcacagaa tatggtcgtg cgtgttcgcc
     5221 accagcagtc ggtcgataaa gtcttcttct ttaatacgtg cggcagattt acctttcccg
     5281 ccacgacgct gcgcttcgta ttcagaaagc ggctgatatt taacgtagcc ctggtgagag
     5341 agcgtcacga ccacatcttc ctgggtgatc agatcttcca ggttgatgtc tgcgctgttg
     5401 gcggtgattt cagtacgacg tttgtcaccg aactgttcac gaaccagctc cagctcttcg
     5461 cggatcactt ccatcagacg atcggcgcta ccaagaatac gcaacagttc cgcgatctga
     5521 tccagcagct ctttgtattc gtcgagcagt ttttcgtgct caaggccggt cagtttctgc
     5581 aaacgcagat ccagaatcgc ctgagcttgc tgttcggtca ggtagtacag accatcacgc
     5641 acgccgaact caggctccag ccattccgga cgcgcagcat cgtcgccagc acgttccaac
     5701 atggcggcaa cgttgcccag ctgccacgga ttagcaacca gcgcagtttt cgcttctgca
     5761 ggcgtcggcg catgacggat cagttcgatg atcgggtcaa tgttcgccag cgccacggct
     5821 aatgcttcaa ggatatgagc acgatcgcga gctttacgca gttcgaaaat agtacgacgg
     5881 gtcaccactt cacggcggtg acgaacaaac gccgcgatga tgtctttcag gttcatgatc
     5941 ttcggctgac catggtgcaa tgccaccatg ttgataccga aagaaacctg caactgggtc
     6001 tgggagtaga ggttgttgag cacaacttca ccgaccgcat cgcgtttcac ttcaatcacg
     6061 atgcgcatac cgtctttgtc agactcgtca cgcagcgcgc tgatgccttc cacgcgtttt
     6121 tcttttacca gttccgcaat cttctcgatc aggcgcgctt tgtttacctg atacggaatt
     6181 tcgtggacga taatggtttc acgtccggtt ttggcgtcaa cttccacttc agcacgggcg
     6241 cggatatata ccttgccgcg accggtacgg taagcttctt caataccgcg acgaccgtta
     6301 atgattgccg ccgtcgggaa gtccggcccc gggatgtgtt ccatcagccc ttcaatgctg
     6361 atgtcttcat catcgatata cgccagacaa ccgttgatga cttccgtcag gttgtgcggc
     6421 gggatgttgg ttgccatacc tacggcgata ccggaagaac cgttcaccag caggttagga
     6481 attttggttg gcatgacgtc gggaattttt tccgtgccgt catagttatc aacgaaatcg
     6541 accgtctctt tttcgagatc ggccatcagt tcatgggcaa ttttcgccag acggatttcc
     6601 gtataacgca ttgccgccgc agagtcgccg tcgatggaac cgaagttacc ctgaccgtct
     6661 accagcatgt aacgcagcga gaatggctgc gccatacgga cgatcgtgtt ataaaccgcc
     6721 gagtcaccat ggggatggta tttaccgatt acgtcaccaa cgacacgggc agatttttta
     6781 taggctttgt tccagtcatt gcctagtacg ttcatggcgt aaagtacgcg acggtgtacc
     6841 ggcttcaggc catctcggac atctggcagc gcacggccaa caatgaccga catcgcataa
     6901 tccagatagg agctctttag ctcttcctca atgttgaccg gtgtaatttc tctcgcaagg
     6961 tcgctcatct aaccgctatc cctctactgt atcccggatt caaaggtcgc aaattataac
     7021 acagccgcgc agtttgaggt aaacctatac gctttattca catccaatgc ctgatatact
     7081 cgtttgtctt gccaattacg gagtagaagt gccaatgaat gccgaaaaat cgccggaaaa
     7141 ccataacgta gaccacgaag agatcgctaa atttgaagcc gtcgcctccc gctggtggga
     7201 tcttgaaggt gagttcaaac cgttgcaccg cattaacccg ctgcgtctgg gctatattgc
     7261 cgagcgtgct ggcggtttat ttggcaaaaa ggtgctcgat gtcggttgtg gcggcggcat
     7321 tctggccgag agtatggcgc gcgaaggcgc gacggtgacc ggtctggata tgggctttga
     7381 gccattgcag gtggcaaagc tgcacgcgct ggaaagcggc attcaggtgg attacgtgca
     7441 ggaaaccgtg gaagagcacg cggcaaaaca tgccgggcag tatgatgtgg tgacctgcat
     7501 ggagatgctg gagcacgtac ccgatccgca gtcagtggtc agagcctgtg cgcaactggt
     7561 gaaaccaggc ggcgatgtct ttttctcgac gcttaaccgc aacggcaagt catggctgat
     7621 ggcggtggtt ggcgcggaat atattttgcg catggtgccc aaaggtacgc atgatgtgaa
     7681 gaagtttatt aaaccggcag agctgcttgg ctgggtggat cagaccagtt taaaagagcg
     7741 acatataacc gggctacatt acaacccgat cactaattcc tttaagctcg gccccggcgt
     7801 ggatgtgaac tatatgctgc acacgcagaa taagtgaggt tgatgtttgg ccgcgccaat
     7861 gcctgatgcg acgcttgcag cgtcttatca ggcctacaaa tgctctccgt aggccagata
     7921 aggcgtttac gccgcatccg gcaattgtga tggtcagaca gcgattacca tttcaccgtc
     7981 atcgacaaaa accctgccgt ctgggcaaaa tcatcactcc ctttctgcca cgccacgctg
     8041 ccgcgcaggg acactcgctg actgatattg cccgtgactc ccacttttat ttcaccccgt
     8101 agcttcaccg catcgtcact gatagtgctg ccatcttctt caatttccgt cgcatgggga
     8161 tcgtgatagt aattcagatc taatgttggt gtgacaccaa ccgcggtacg ccattcgctg
     8221 tgtaaaccca gccgcgtctg aatatcatcc ccctgcgatt gtgacacgcg cgcatggtta
     8281 gcggcggtaa aatcatcctg ctgcacgccc tgataaatca cctgcgcctg cggttcaatc
     8341 accacaccac gccccggtaa ccactgatac ccgacttcca gcgaggcgat aatccccgac
     8401 gaatggtaac gatccacgcc atcttcatgt tcggaaacat cattgttaaa ccacgcgtat
     8461 tgcagccagc tatccagcca ggccccctgc ttctgattac cgtgctgata ccagcttgag
     8521 gtcagcccaa cggcataacc gtggttctgg ttatcggcgc gagttccggt catattcgag
     8581 cggctgtcgc cctgattatc actgtagcca ccaaccgccc caagcatcca ctcgccatcc
     8641 gtgccccagc gcccgctaaa caagccgccg ctaagctgca ccgtagaagt gtcttcctgc
     8701 tgagccagtt gccctgccgc cgtgtaatga taacgtccgc cgataacacg taaattcagc
     8761 gtctgaccat cgccaccagc gtgatcgcgt cgctccatca taaacgcctg atttgccgcc
     8821 cgcaggttat tgagataacc gccaactttg gcattcagca ccggctggta agcaggcgta
     8881 ggggccgggt cgggggttgg gtcaggatcc ggcgtgggat caggatcggg agtcgggtct
     8941 ggctcaggtg gcgatggcgg cgttacttct tgcgatcgca gataccagtc gttgttatct
     9001 tccactagcg tgtagtcata cgctcccatg ttgacgtagc cactgccagc cagactgaac
     9061 tgtgcattgt tttgaaactg cgtgggatca gctgcgaaat caaccacttt aatgcctgtc
     9121 gatgtcggct caccaatccc tgtaatggag ttaaccacca cagttgtgtt gccagcagta
     9181 ttaccgttca tcaccaattg atcgcttacc gagtcatcgc cgtttaattc gctgtcgaat
     9241 agtagcgtac cgccgccggt ataatcaccg tttaccgtca gcgtatcgcc agcgacgcca
     9301 ttttgcaggc taaccgcacc ggacgtattg gtcagatcac cattaaccgt cacgtcagca
     9361 agataggtca ggctgtcagt ttcaaggttg gtattaagct cgcctgtcaa cgtcacgcca
     9421 tcggtagcat ttaccagcgt cccatcgccg ttaaatagtg aggcattaag ggcgacggaa
     9481 gtatcctgcc cggtcagacg caaaaccgta ccatcgctga tgtcgatagt gccgctggaa
     9541 gtagcatcaa ttgactgaat atcctgatcc gcgccagtaa cgaacgttgc cccatcacca
     9601 acccacagtg acgaagcata aggaaagata tccgcaacat cgcctttcag ggtgccttct
     9661 tcgacacgta ccgccgactg cgttgtaccg ctggcggtca gctccagcgt acccgccccc
     9721 gttttagtaa atgtgctacc ctcatcctga tgctgcccgc tgctgtcagc catcagtgcg
     9781 ccccactgcg tgtctacccc cgcatcaact gccacttcac cgtcggcgcg catttcaata
     9841 tcacgcccat gctcagtcgt cgtagagcga gtatcgacat tggcagtaat ctccatcacg
     9901 ctttcttgtt acttatcggt aaagataacc tggcggttat agtgggtatc tccaagctgc
     9961 gagttgtcgc tcaccatcag cgtaccggag gcgatttgtg ttgtgcccaa ataactatta
    10021 ttgtttgcca gacttacctg cccgtcgctc gcatcgacca ccacgtagac gccatctttc
    10081 ccgtcgccgc cgatatgcaa attgtcgcca cctgcaagat ctaccacatc ctgactgccg
    10141 ataaccgttc cgctgctgcc actgacttca aggccatcat tgtatgatgt gccgctctgc
    10201 caagtggaga aatcagagag atcgagcaca ccgccgttta acacgatcga ctgcggatcg
    10261 tcctggagag cggtaagatc tgccgcgtcg ccttccagcg aaagcaccgc accatcatca
    10321 acgactatat cgccggttag cgccatcgac tgcgcccctg ccagcacgta gctgccgttt
    10381 tgcgcaatgg tgagctgacc agcaccttcg atgatgccag caaaactgcc ctgattaaca
    10441 gtaacgttgc caccagcatc gatatttaaa gtgccattct gaaagcccgt caatgcgtgc
    10501 acaaaagttt gttgggtcga gccaacgttt agctcagcct gattctgata ctgatcaata
    10561 ctccctatcg tcagaccgta gcagtcttgc ggatcgtcct ggcaatgcgt atcgccgaca
    10621 ttcatcaggg agttgctgcg acccagggta acttcaccgt tttcaatctg catctcacca
    10681 gtaaagtcat tgttatctgc attaagtacc agatcgccgg aacctgtttt ggtgattaac
    10741 ccggtaccag caatagagtc aacagctccg tcattctctg tattgccgat aaccagcgtt
    10801 tttccgtcgg caatatcaaa ggtaacttca cttaagccga gatacataaa gccacccgcc
    10861 gcagtggagg gaccatctcc atagcctgct gcgctattgt tctcatcgac taacacgccg
    10921 ccgttctggc tgtagctgtc atcaacagaa atatcaataa gatagggagc cgtcgcgcta
    10981 ttggtatata tcgccccgcc ataaccttcg gctgtgttat ttgtaaaggc ggtgttattt
    11041 attatcgtat aacctgaagg atgcttgctg tcgctattat tatcggtaac atcgattgcc
    11101 ccgccatcgc catcactgta acttgttgat gtatatgcct ggttattgtt aaaaacatca
    11161 tcacttaaat agacgtcatt attaatggta
//
LOCUS       DAJTUN010000119        11159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTUN010000119 DAJTUN010000000
VERSION     DAJTUN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.119"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            9..1616
                     /gene="trg"
                     /locus_tag="OO470_003188"
     CDS             9..1616
                     /gene="trg"
                     /locus_tag="OO470_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6252347.1"
                     /translation="MHHIRLVPLFACILGGILVLFALSSALAGYFLWQADRDQRDVTA
                     EIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESEIKQSQQGYRAY
                     QNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHESEQIRPLDNAY
                     TDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLITFMVLRRIVIRP
                     LQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVGTVRQGAEEIYR
                     GTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHASKLAQEASIKA
                     SDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAVEAARAGEQGRG
                     FAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTMSTIVDAVASVT
                     HIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVSLEEQAARLTEA
                     VNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1654..2577)
                     /locus_tag="OO470_003189"
     CDS             complement(1654..2577)
                     /locus_tag="OO470_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6252348.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            2794..4137
                     /locus_tag="OO470_003190"
     CDS             2794..4137
                     /locus_tag="OO470_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6252349.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4362..6017
                     /gene="opgD"
                     /locus_tag="OO470_003191"
                     /gene_synonym="mdoD"
     CDS             4362..6017
                     /gene="opgD"
                     /locus_tag="OO470_003191"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6252350.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6157..6381
                     /locus_tag="OO470_003192"
     CDS             6157..6381
                     /locus_tag="OO470_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6252351.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6444..6980
                     /gene="rimL"
                     /locus_tag="OO470_003193"
     CDS             6444..6980
                     /gene="rimL"
                     /locus_tag="OO470_003193"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6252352.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(6975..7955)
                     /locus_tag="OO470_003194"
     CDS             complement(6975..7955)
                     /locus_tag="OO470_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252353.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8079..9071
                     /gene="tehA"
                     /locus_tag="OO470_003195"
     CDS             8079..9071
                     /gene="tehA"
                     /locus_tag="OO470_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6252354.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9068..9661
                     /gene="tehB"
                     /locus_tag="OO470_003196"
     CDS             9068..9661
                     /gene="tehB"
                     /locus_tag="OO470_003196"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6252355.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            9964..10632
                     /locus_tag="OO470_003197"
     CDS             9964..10632
                     /locus_tag="OO470_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6252356.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(10650..11156)
                     /gene="tnpA"
                     /locus_tag="OO470_003198"
                     /pseudo
     CDS             complement(10650..11156)
                     /gene="tnpA"
                     /locus_tag="OO470_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2876 a         2610 c         2813 g         2860 t
ORIGIN      
        1 taggtttttt gcatcacatc aggctggttc cgttatttgc ctgcattctt ggcggtatct
       61 tagttctatt cgcattaagt tctgctctgg ctggctattt cctctggcag gccgatcgcg
      121 atcagcgtga tgttactgcg gagattgaga ttcggaccgg gttagcgaac agttcagatt
      181 ttttgcgttc agcccggatc aatatgattc aggccggggc tgcgagtcgt attgcggaaa
      241 tggaagcaat gaagcgaaat attgcgcaag ccgaatcgga gattaaacag tcgcagcaag
      301 gttatcgtgc ttatcagaat cgaccggtga aaacacctgc tgatgaagcc ttcgacactg
      361 aattaaatca acgctttcag gcttatatca cgggtatgca acctatgttg aaatatgcca
      421 aaaatggcat gtttgaagcg attatcaatc atgaaagtga gcagatccga ccgctggata
      481 atgcttatac cgatattttg aacaaagccg ttaagatacg tagcaccaga gccaaccaac
      541 tggcggaact ggcccatcag cgcacccgcc tgggtgggat gttcatgatt ggcgcgtttg
      601 tgcttgccct ggtcatgacg ctgataacat ttatggtgct acgtcggatc gtcattcgtc
      661 cactgcaaca tgccgcacaa cggattgaaa aaatcgccag tggcgatctg acgatgaatg
      721 atgaaccggc gggtcgtaat gaaatcggtc gcttaagtcg tcatttacag caaatgcagc
      781 attcactggg gatgacagta gggactgttc gacagggcgc ggaagagatt tatcgtggca
      841 ccagcgaaat ttcagctggc aatgcggacc tgtcatctcg caccgaagaa caagcggcgg
      901 ctatcgaaca aactgccgcc agcatggagc aactcactgc gacggtgaaa cagaatgcgg
      961 ataacgcgca tcatgccagc aaactggcgc aagaggcttc tattaaagcc agcgatggcg
     1021 ggcagacggt ttccggtgta gtaaaaacga tgggcgctat ctccacgagt tcgaagaaaa
     1081 tttctgagat caccgccgtc atcaacagta ttgctttcca gacgaatatt ctggcactga
     1141 atgctgccgt tgaagccgcg cgagcgggtg agcaagggcg tggatttgcc gttgtcgcca
     1201 gcgaagtacg gacactcgca agccgcagcg ctcaggcagc gaaagagatt gaaggcttga
     1261 tcagtgaatc agtcaggtta attgacctgg ggtcggatga ggtggcaacg gcagggaaaa
     1321 ccatgagcac tattgttgat gccgtcgcga gtgtcacaca tatcatgcag gaaatcgccg
     1381 ccgcctcgga tgaacaaagt agaggcataa ctcaggttag ccaggcgatt tctgaaatgg
     1441 ataaggtgac gcaacagaat gcttctctgg tagaagaggc ctcagcagcg gcggtgtccc
     1501 ttgaagaaca ggctgcacga ttaactgagg cggtgaacgt attccgtctg aacaaacatt
     1561 ctgtgtcggc agaacctcgc ggagcgggtg aaccagttag tttcgctacg gtgtgaaaat
     1621 gttcaaggag ggatcgacag atcccttcac ctttcagaac ggcattgatt ttcgaatagc
     1681 gttaatcatc aactggcaac cagaagagaa cgtcgcatct acgcgggtca gtattccaat
     1741 cggttcgcct gcaccatgtc ccggaacagg cagggccacc agcgtggcat gacgcaggtc
     1801 gtcttttaca gcgccagaag ggacaaacca cacgtaatcg tattcaacgg taagttgacg
     1861 agatagcgaa gtagacagcg tttcgataca acccgaagga attttacagc cctggctctg
     1921 cactaatgca tctgaatgct ggcgtggcgc agtgccttct ggtgatacaa cgaccggcca
     1981 ttccagcacc cggcttagcg ttacgttctc ctgaagtagc gggtgattag ggcggacaac
     2041 cagcttcaac gattcaagaa acagcagttc gtaattaagc ccggtcatca gttcaggatc
     2101 tgacatccga ccaatgccga tatcgatttc cccggttttt aaacccgcca gaatcatagg
     2161 gttactcatt gtcgcaactt gcaaggtcgt ctctttttgt tgctgatgaa actgacctat
     2221 aaccgaaggt aatatcccca gtgccgcagt aggtagtgca ccaaccctga cgacatcatt
     2281 attaagacct tctttacgat gaagcgactg tccggcagtg ttgatggcgt caagaactct
     2341 gactgcatgc gttaaaaatt gttcgccggg taaggtaagt tgcgccccct gacgaccacg
     2401 ctcaaacaag cgagcgcccg tcagctgctc cagttcattc aatgtattag agagcgcagg
     2461 ttgactcaaa ttaagggttt cagctgcgcg ccccaaagtt ccttgttgtg cgacagctac
     2521 gaatgtatga aggtggcgca aacggatgcg ctgactaaac agactatttt tttccataag
     2581 cgatgttaaa aacgaagcgg tgtcgctgac aagtgaagtt gtttgattat gataacttga
     2641 ttgcaaaata ttattaataa ttaaagcaat tatgttacag caaaatggat aatattgatg
     2701 ttttcgcggc gagatcacag tttgtaaatt cttcccgcca gagtgaatgc ggttacctac
     2761 actccagatt actgaccact ggaggcagac actatggcga acagcatcac ggcggatgag
     2821 attcgggaac agttttcgca ggcaatgtca gccatgtacc agcaagaagt tccgcaatat
     2881 ggcacgctgc tggaactggt agctgatgtg aatctggctg tgctggaaaa caatcctcaa
     2941 ctgcacgaaa aaatggtaaa tgcagacgag ctggcgcgac tgaatgttga acgtcatggg
     3001 gcgattcgcg ttgggactgc acaagagctt gctactcttc ggcggatgtt tgccattatg
     3061 gggatgtacc cggtgagcta ttacgatctc tcgcaggcag gggtgccggt acattcgaca
     3121 gcatttcggc ccattgatga tgcttctctg gcgcgtaatc ccttccgcgt ttttacatcc
     3181 ttactccgcc ttgagcttat cgagaacgaa attttgcgcc agaaagcggc ggagattctg
     3241 cgtcagcgcg atatcttcac cccacgttgt cgacaactgt tagaggaata tgagcagcag
     3301 ggcggtttta acgaaacaca ggcacaggag tttgtgcagg aagccctgga aacgtttcgc
     3361 tggcaccagt tagcaacggt agatgaagaa acctatcgcg ccttgcacaa cgaacatcgg
     3421 ttgattgctg atgtggtctg ttttcctgga tgccatatca accacctgac gccacgtacg
     3481 ctggatattg accgggtgca gtcgatgacg cctgaatgcg gaattgaacc caaaattctc
     3541 atcgaggggc cgccgcgccg cgaggtaccg attttactac gccagaccag ctttaaagca
     3601 ctggaagaga cggtgttgtt tgcggggcag aaacagggca cgcataccgc gcgctttggt
     3661 gaaattgagc agcgtggcgt ggcattaacg ccgaaagggc gacaactgta tgatgatctt
     3721 ctgcgtaacg ctggaaccgg gcaggataat ctcactcacc aaatgcattt acaggaaacc
     3781 ttccgcactt ttcctgacag tgagttttta atgcgtcagc aagggttggc atggttccgg
     3841 taccgtctga cgccttcggg tgaggcgcat cgtcaggcga ttcatcctgg agacgatcca
     3901 cagcccttaa ttgaacgtgg ttgggtagtg gcgcaaccca tcacctatga agatttcttg
     3961 cccgttagcg cggcggggat cttccagtca aatctgggta atgaaacgca gacacgcagt
     4021 cacggtaatg ccagtcgcga agcatttgag caggcgttgg gttgtccggt tttggatgag
     4081 ttccagcttt accaggaagc ggaagaacgc agtaaacgtc gctgtggttt gctttaaatt
     4141 ctgaccatcc gcctttgcaa aaatttgcct gattttacaa acgaatcagg ctcatcccat
     4201 cgacataaaa aaaatgccga tttatgcata ttctctcagt tcaacaattg gattattaat
     4261 aaatattgtc tagagtgagc ggtcataaat aagcactttc ttgccgctga aaacgaccag
     4321 cgcgggacca ttcacaacac cagaaggact cactttcagg tatggatcgt agacgattta
     4381 ttaaaggttt aatggctatg gccgccgtgt gcggtaccag cggcattgct tctctttttt
     4441 ctcaggcagc attcgcggca gattctgata ttgccgacgg gcaaacccag cgttttgact
     4501 tctccattct acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt ggtgcgcctc
     4561 gtccgttacc tgacacgctg gcgacaatga cgccgcaggc ttataacagt attcaatacg
     4621 acgccgaaaa atcgctctgg cataacgttg agaaccgtca actggacgct cagttcttcc
     4681 atatgggaat gggattccgt cgccgcgttc gtatgttttc tgtagatcca gcaacacatc
     4741 tggcgcgtga aattcacttt cgcccggagt tgttcaaata caacgatgca ggtgttgata
     4801 ccaaacaatt agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg tttaaagccc
     4861 ccgaactggc gcgccgtgat gtagtatcat ttctcggcgc gagttatttc cgcgccgttg
     4921 atgatacata tcaatacggt ttgtcggccc gcggcctggc gatcgacact tacaccgaca
     4981 gtaaagaaga gttccccgac tttaccgcct tctggtttga tacggtaaaa ccgggggcaa
     5041 ctacctttac cgtttatgcg ttgctcgata gcgccagcat tactggtgcc tataagttca
     5101 ctatccattg tgagaaaagt caggtgatta tggatgtgga aaatcacctg tatgcgcgca
     5161 aagacattaa acagctgggc atttcgccga tgaccagtat gttcagctgc ggtactaatg
     5221 aacgtcggat gtgcgataca attcatccgc aaattcatga ctctgatcgt ctgtccatgt
     5281 ggcggggcaa cggcgagtgg atttgccgtc cgctgaataa tccgcaaaaa ttgcagttca
     5341 atgcttacac cgacaacaac ccgaaagggt ttggtttatt gcaactggat cgtgacttct
     5401 cccattatca ggacattatg ggctggtata acaaacgccc aagtctgtgg gtggaaccgc
     5461 gtaacaagtg gggtaagggc accatcggcc tgatggaaat cccaacaacg ggcgaaacgc
     5521 tggataacat tgtctgcttc tggcagccag aaaaagctgt aaaagcaggt gatgagtttg
     5581 cattccagta tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg
     5641 ttatggcgac gcgtaccggc atgggcggtt tcccggaagg ttgggcgcca ggtgaacact
     5701 atcccgaaaa atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat ctgaaagctg
     5761 ccgcgccaaa aggcattgag ccggtgatta cgctttccag tggggaagcg aagcaaatcg
     5821 aaattctcta tattgaaccc atcgatggtt atcgtattca gtttgactgg tatccgactt
     5881 cggactccac tgatccggtc gatatgcgga tgtatctacg ttgtcagggg gacgctatca
     5941 gtgaaacatg gctgtatcag tatttcccgc cagcgccgga taaacgtcag tatgttgacg
     6001 accgcgtgat gagttaatcg ttttttcttc ggcaccttct tcgggaggtg ccgtctggtt
     6061 aaacacgatc ccgctcgcat ttttccctaa gttaaatgag taatctgatg gtgtgtattt
     6121 cagatacacc gtgtcagcca ctaacaggga gtgcgtatgt ttccagaata ccgagattta
     6181 atatcccgtc tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa acacaataaa
     6241 cttgatcatg aaattgccag aaaggaaggt tccgacggtc gagggtacaa tgcggaagtg
     6301 gtccgcatga aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat cctgcagcag
     6361 gagagcgtca aagaggtgta aactttccta agccgccagc caggcggctt ttttaacaac
     6421 agcatggatt gactggagat aagatgactg aaacgataaa agtaagcgaa tcacttgaat
     6481 tacatgctgt tgcggaaagt cacgtcacac ctctttatca gttaatctgt aaaaataaaa
     6541 cctggttaca gcagtcgcta aactggccgc agttcgttca gagtgaagag gacacgcgaa
     6601 aaacggtgca gggtaatgtg atgttgcatc aacgcggcta tgccaaaatg ttcatgattt
     6661 tcaaagaaga tgaacttatc ggcgttatct cgtttaatcg tattgaacca ctgaataaaa
     6721 ctgctgaaat cggctactgg ctggacgaat ctcatcaggg gcaggggatc atttctcagg
     6781 cgctgcaggc attgattcat cattacgctc agtctggtga acttagacgc ttcgtgatca
     6841 aatgtcgggt ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt tttatccttg
     6901 aaggttgcct gaaacaggct gagttcctga atgatgccta tgatgatgtg aacttatacg
     6961 cgcgtattat cgattcataa taaccctgcc agcggcgttc gcgtaatgcg ctcctcgccg
     7021 ttaatgactt ttggaccacg gacatgaacc gtgtcgccat caaaggcttc aacgacggcg
     7081 tggtcggtta actccacatg gttttcaatt atcaccgcac ctgtaatacg gctctcacct
     7141 tgtattacaa cgtgctcgtc gagcagaatc ggtccaccac gtaccacggc atttccgcca
     7201 atcaagacat gatgttttaa cacacaatta ccttccacaa tggcatattc cgccacctgc
     7261 gaactgtaat gaatcgtcgg aatggcatct tcttctatgc cagctttcac ctgcgcgtga
     7321 ccgtagactt tagcgcaatc gcatagccag acgttgttct cttcattacc ttcaacactg
     7381 gcaaaatcaa aaacttcggc gcgatgttca ataaaagcat accggacgac ggcatcgcca
     7441 taaatttgtg cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc ataaatttgg
     7501 agcaggagtt gatggtcggg cgttaagcct tgtgcggcga cgatcataga atgttgatca
     7561 attaaggcgt gaccaaatat tctacattgc acgtaaacta atgagtcatg aatggtgaca
     7621 ctgtcactta tatatgcacc ctggctgatt tcactattat cgatccagac gttatccgtt
     7681 gcgtaaacct ctccccacag cacgctggtg ccggtgatac gcgtattacc ggaaatcacc
     7741 gttccgccga acgcaatagc attctggtcg taaatccagc aatttccttc ctgtgctaac
     7801 accgtttcgc gatcgatcca gccgccagcg gttcctgcta tcacatcgtt aaaatcgcta
     7861 atggcgataa tctgccgtaa taacacactt tttttagtgc catcttcctg ataactaaag
     7921 gctcgctgtt cttcacttag gcgatatttg cgcatagcgt tttcccacag gtgacttact
     7981 ataaccgtag caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt
     8041 caaatatact ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca
     8101 atttgccggc aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc
     8161 gctatgccag ccaggtttgg caggtcagcc actggttagg ggatgggctg gtgattctgg
     8221 cgatgatcat ctggggatta ttgactagcg catttattac ccgactcata cgctttccgc
     8281 atagcgtgct ggcggaagtt cgccatccag tgctgagcag ttttgtgagt ttgtttccgg
     8341 caacgacgat gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgca
     8401 tgttcagttt tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat
     8461 ggcgcggatc tcaccctgaa gaagctacca cgcctggact gtatctgccg acagttgcca
     8521 acaactttat cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt
     8581 ttttaggcgc aggcgttttc tcatggctaa gccttgaacc ggtgatcttg cagcgtctgc
     8641 gcagttcggg agaattaccc acggcactgc ggacatcact cggcattcag ctcgctcctg
     8701 cgctggtggc ctgtagtgcc tggctgagcg tcaacggcgg cgagggtgac acgctggcga
     8761 aaatgctttt cggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt
     8821 atctctccca gccatttaat gcttcattct ggagtttctc gttcggcgta tctgcactgg
     8881 caaccaccgg tttgcatctg gggagtggca gcgataatgg atttttccat acgctggcgg
     8941 tgccgctgtt tatctttacc aattttatta ttgcaatact gctcatccgt acttttgcgc
     9001 ttctgatgca gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg
     9061 acaaagaatg atcattcgtg acgaaaacta ttttactgat aaatatgaat taacccgcac
     9121 acactctgaa gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg
     9181 ctgtggcaat ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg
     9241 ggataaaaat gccatgagta tcgccaacgt cgagcgcatt aaatccattg aaaatctgga
     9301 taatttacac acccgggtcg ttgatctgaa taacctcaca tttgatggac agtacgattt
     9361 tattctttcg actgtggtgc tgatgttcct tgaggctaaa accatcccag ggttgattgc
     9421 caatatgcaa cgttgcacta aacctggtgg ttacaacctg attgtggcgg cgatggatac
     9481 cgctgattat ccatgtaccg tcggcttcac gtttgccttc aaagagggag aattacgtcg
     9541 atattacgaa ggctgggaga gggtgaaata caatgaagac gtcggcgagc tgcaccgcac
     9601 cgacgccaac ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg
     9661 acccggtaag cacaaaacgc gtgaaaattc cccacgctga gatgatttac tgttcttctt
     9721 ttcggtaagc atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt
     9781 tagcagctta acttactgaa atttaactac tgatgattga cttagcccct ttttcggcat
     9841 tgactatgtc gtctgaaaag gggctgaaaa atttattttc accaacactt tttttgccac
     9901 aacacgaagc ggcgcttttt gctataactt agaaagtaat ataatcatct caggaaacta
     9961 ttcatgcgta ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct
    10021 ggttgtgcct ctaaaatcac ccagccagat aaatattctg gttttttaaa caattactct
    10081 gatttaaaag aaacaacctc agcaacaggt aaacctgttt tacgttgggt agacccgagt
    10141 tttgatcaaa gcaaatatga cagcatcgtc tggaacccaa tcacttatta tccggtacca
    10201 aaaccttcta cccaggtagg gcagaaagtt ctggataaaa ttttgaacta taccaacacc
    10261 gaaatgaaag aagcgatagc gcagcgtaaa ccagtggtta ccaccgctgg gccgcgtagt
    10321 ctgattttcc gtggggccat taccggtgta gataccagca aagaagggct gcaattctat
    10381 gaagtggttc ctgttgcatt agtggttgcg gggacgcaaa tggctacagg ccaccgtacc
    10441 atggatactc gcctctattt tgaaggtgag ctgattgatg cagcgactaa taaaccggtt
    10501 atcaaagtcg ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac gccaatggct
    10561 ttcgaaaata ttaaacaagt tattgatgac atggcgaccg atgccaccat gtttgacgtt
    10621 aacaaaaagt agttccagac gcgccatcgt tagatggcgc tttttatccg gtgcgccgta
    10681 aaaccccatc cttcagggcg gggatataag gcgcggtttt ccacctgacc aggtgtttgc
    10741 tgttgttcaa tgtattggcg gatgacggat attggcgcac ctccgcagct actggcaaaa
    10801 tagccaggac tccacaaaac gcctttgtag taatacctga ctgcaatatc tggtcgatct
    10861 cgtcgcagta acctacccga tacgcctttg aggctgttta ccagactgga tatcgccagt
    10921 ttgggaggtt agttaattaa caaatggacg tgatctggtt cgccatccat ttcaaccagt
    10981 tcagcttcaa aataagcaca tacatttgaa aagtaagtgc gtagtttttc tgtcgcgtca
    11041 tggtcaaaaa tctggcgtcg gtatctggtg acaaagacta ggtgaacatg catcaggaaa
    11101 acacaatgcc ttccacgccg gatatcggtt tcttttttca cagaccaaag tatcatttg
//
LOCUS       DAJTUN010000120        11139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTUN010000120 DAJTUN010000000
VERSION     DAJTUN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.120"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO470_003199"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO470_003199"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO470_003200"
                     /pseudo
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO470_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO470_003201"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO470_003201"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6252357.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO470_003202"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO470_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO470_003203"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO470_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6252358.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO470_003204"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO470_003204"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6252359.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO470_003205"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO470_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6252360.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO470_003206"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO470_003206"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6252361.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO470_003207"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO470_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6252362.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO470_003208"
     CDS             9553..10332
                     /locus_tag="OO470_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6252363.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO470_003209"
     CDS             10322..11131
                     /locus_tag="OO470_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6252364.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
BASE COUNT         2667 a         3026 c         2794 g         2652 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag caccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 acaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgagaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaatc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcagttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaacccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggtgggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggttt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgct
//
LOCUS       DAJTUN010000121        11106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTUN010000121 DAJTUN010000000
VERSION     DAJTUN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.121"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(316..465)
                     /locus_tag="OO470_003210"
     CDS             complement(316..465)
                     /locus_tag="OO470_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6252365.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITA"
     gene            complement(511..1011)
                     /gene="eutK"
                     /locus_tag="OO470_003211"
     CDS             complement(511..1011)
                     /gene="eutK"
                     /locus_tag="OO470_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6252366.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(1024..1683)
                     /gene="eutL"
                     /locus_tag="OO470_003212"
     CDS             complement(1024..1683)
                     /gene="eutL"
                     /locus_tag="OO470_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6252367.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            2865..3656
                     /locus_tag="OO470_003213"
     CDS             2865..3656
                     /locus_tag="OO470_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252368.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            4376..4771
                     /locus_tag="OO470_003214"
     CDS             4376..4771
                     /locus_tag="OO470_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6252369.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            4789..5052
                     /locus_tag="OO470_003215"
     CDS             4789..5052
                     /locus_tag="OO470_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252370.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            5075..5617
                     /locus_tag="OO470_003216"
     CDS             5075..5617
                     /locus_tag="OO470_003216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252371.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            5756..6043
                     /locus_tag="OO470_003217"
     CDS             5756..6043
                     /locus_tag="OO470_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252372.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            6082..6288
                     /locus_tag="OO470_003218"
     CDS             6082..6288
                     /locus_tag="OO470_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252373.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            6600..7085
                     /locus_tag="OO470_003219"
     CDS             6600..7085
                     /locus_tag="OO470_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6252374.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            7066..7344
                     /locus_tag="OO470_003220"
     CDS             7066..7344
                     /locus_tag="OO470_003220"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252375.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            7337..8797
                     /locus_tag="OO470_003221"
     CDS             7337..8797
                     /locus_tag="OO470_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6252376.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            8831..9640
                     /gene="murI"
                     /locus_tag="OO470_003222"
     CDS             8831..9640
                     /gene="murI"
                     /locus_tag="OO470_003222"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6252377.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(9759..10453)
                     /locus_tag="OO470_003223"
     CDS             complement(join(9759..10207,10207..10453))
                     /locus_tag="OO470_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6252378.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(10972..>11106)
                     /locus_tag="OO470_003224"
     CDS             complement(10972..>11106)
                     /locus_tag="OO470_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6252379.1"
                     /translation="QLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTVKSLAFPS
                     "
BASE COUNT         3205 a         2457 c         2479 g         2965 t
ORIGIN      
        1 aaagttagcc tgacaggtga tgacatcttc agacagcact acgcccagga tcgtataatc
       61 gtccggcgta cttaattcaa actcggttcc accggggcgc gtcgctattt ctgcgcttcc
      121 cagacattgc gaaccgataa atccctgctc accgcgcgtc gccggaatgc caaaccagaa
      181 cgagttcggc cagaccaggc acgactgacg cagcgccaga ccggtgtatt cacgaaaaac
      241 ctgaatatca tcgagtagaa tttccgtaaa ttcaccatga aacttgcccg gatgcagctg
      301 atcgtaaatc tgctgctagg cggtaatcgt caaagcatgt tcatagacat ccgttgtctg
      361 tcgttgatga acattatcca cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg
      421 ttcatgataa agatggtgca aattggctgt acgggtcttt ttcatgatgt taatgccggg
      481 tgttgtagga cacccggcac ctccgacagg ttaatggggc ttgagacgat aacgactact
      541 gcgtttacgt aacgtcccgg cagaaaagag ctgttcgagc gcatttctgg ctttttccag
      601 cggccagcca aagtgggcgg ccacttctcc tgccgtcatt ccctgacata ctgatgttaa
      661 cagcgccaac aactcgtcag cagattccgc gacgataact ggcgcgtcgg gttcctttac
      721 gggttgcttc ggctggcggt taaagccagt aaccagccac tgggtgtcat cgtctggccg
      781 accgatctcc ttacggctga tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc
      841 cagcgccgca cgacacgccg ccagatcgcc ttccaccacc agcgttaagc gaccagggtc
      901 aagcacttcg tgactgagca gacgaacgtt agctgctttg agcatggcat ccgctgcatc
      961 tatcgcggcg accatgccgt ccacttccag caatcccagt gcattgatca tcggcaacct
     1021 ccgttacgca cgctggattg gattacgcgc gatttccagc actgcatcgg taaaggcgtt
     1081 acaggctgct ttacacgcgg cctggctacc ggttaaaaat gctgccgagt agttggtttc
     1141 agacggtggc gggacgtagg tcaccagctg tacgtcggca gatttcaacg cggcatcaat
     1201 gccgtaggtc gcttccagcg gcggtgccac cagatacgcc atcggatcgc caagcgtgat
     1261 cccggcggtt gatgaaagat aagaaccggt acgcgaaacc acatgcgcca ggaaggcggt
     1321 gttttctgcg tcattggccc actggaaagc tgcgccattt tcaatatgtg caaccatcgc
     1381 atccagaccg gcacgcactt ccgccgggtt tggcccacca agcataatca gcacttcacc
     1441 ggcagtcggt gacgggccgt gtgccgctcc cgcatacagc gagcgaccat ataccacttc
     1501 gaccatcgct tgtttggtcg cttcgtcagc cgcaatgtag gttacgtcgt cagaatcggc
     1561 agaaatcagc ccgaggctac gaatatgcgg cggcaatttt agttcacgcg caaattcagc
     1621 gttaacggag gcaatcaccc gcatggcggt taccgacggt cgaatcaaat ctaaagctgg
     1681 catgatgcct ccttatcggg tcatgttgat gccggatgct ttctgctcca gcatacgttt
     1741 cgccaaatcc acaatgacgg cacagcagca cgaaagagaa gcaaaagtca gctacctggt
     1801 acactaggca gcgggtcagc aaaggggggc aaatgcccct tttttcatgt cagataatca
     1861 gatcattacc tcaataaact aataatcaca tcgcggttac aaccagtttg atagtgatag
     1921 ctcaaaccca gacgtggcgc tggctgcaag caaaataagc cgcaaaaatg cgaccccatg
     1981 tttactagcc gcaaaaatgc ggtcctattt gccagaacct tttattattc aattggttag
     2041 tgcaagaaaa agcgtgatta ctgttcaaga agtatccaga aaggtcatga ttttgacgct
     2101 gaaaaaaaat aggtgggcat ttttgcccac tcactagccg caaatttgcc cactcactag
     2161 ccgcattttt gcggcccaaa gtaataaata cagagaaata ataaatacag atcagcgcca
     2221 agatttttcg tcttttcggc ggaatcatga gactagtgct tacgtcctga taacctccag
     2281 agcagacctt aacttctgtg cagcaggact acgctcattg ccgctcggct aggtcaaatc
     2341 ctcacgataa agctgttcgg attgtgacca taatgttaaa ggactcttta aatcattcct
     2401 tgacgaatta actagctggc ttaagcggaa tagcatgcag gataaaaaca aaaccagctt
     2461 agcagtgggg agagcttcgc ttcgctcgtc tcctggggcc aatgctcaat gaaacgacgt
     2521 aaggcacgga gcgctcacca taggtagaca cataaacatc tgagagcgca ccttctatcg
     2581 cgctacgcgc tcaataaaaa ccaacgacac cgccacactg caagaccaat ggtttttatc
     2641 gcgtatacag accagacagg aaacagcagc aaagcagcgt atagccaggt gcagacagca
     2701 cactcacacg acgcacagca ggcttaagtg atcactcagg aaactttatc cccgccgccg
     2761 atcaccgcga actatcatga ccgcgccaaa ttcgaaagga atggcccata gaacgcgatg
     2821 aacacacata agcgctacac ttacttaacg tcgccacaga gcgattgaga gcgcttaaaa
     2881 tcgatgcgat ttcattaaat ttttctcact cgcctgaatg gtggcgatca ggactgatcg
     2941 caacattgcc ccgcctcgat attgagcgca taatagatac atacattaag cggaaaagag
     3001 ataagtttat tgtgaaaatc agggggttac atatcatgcg catatcagaa caacaacggc
     3061 agatcctcga ctatctggca aaaaacgaat caatcaccgc acgacaagcc gcagatctcg
     3121 tttatggtgg caatgttacc cgcgtccagg ttgacgccgc ccgccgctca ctcatgacga
     3181 tggttagtaa tgggctaatc aggaaacaag gccgcgcttt cgtagctgaa agacatatca
     3241 atcacgctta tagccaggac gttgaagaat tgcgccgcca gatcctttgg gtattcagca
     3301 aacagggtca ggagtatttc ctttctgaac acccaaattt cgccgccaaa gagttaacag
     3361 taagcggatt gtcgtgcgca ctggattatc ttgtcggtga aggttgcgtt acttacaagc
     3421 agctatggca cgccctgaat acgcttgtag aagatggttt actcattgcc acgcgtgaga
     3481 acatcgaata ctggataatg ccgcgttcag gtcgccgcaa tcatgcacgg tatatttcag
     3541 gcaacagata cagaacgccg gactttaatc cgacacagga agatcgggac cgtgccgaaa
     3601 tgatgtgcag ggaacaggag aaaagaatta tggataagtt attttcgatg cagtgaacac
     3661 aagcgggcgc aatgcccgtt tttattgcct tattccacgc ctgacgaatc aatttgatca
     3721 cacctgagaa aatgcatagc cagaagttaa ggccgttata ttgcgattgg gagcgtttta
     3781 aacggtatca atttaattaa ttctgatgtg gtgatttgca ggcaaaaaaa acccgccgga
     3841 gaagcgggaa atagagacat atcgttgtag ttattaacag atcactttga ggtcgagggc
     3901 ggctaaccct cgctaatatt atcccgctat taccttgcgg gtgcaaataa tcttttattg
     3961 ctttgatgat ttaataaata gcactttttg ctaacaaaaa ctgctgtcaa tatatggcca
     4021 aaaatgtcaa cagaaaaaat aaggtttata atattaagta tcacatgggt aatgggttca
     4081 gggaatcgga atccaccgcc tcacggaaat tcaaaatttg cttcttcttt cctccgtggg
     4141 cggcttcttt ttcgtatcga ggtatttatg aaagcaataa cattagaaca gttcgcagca
     4201 atggcgaatg tatcgaccag aacgctgagg cgctggctaa aaaaaccatc aaccggagtc
     4261 cctttgccat ttaccccacg cggtactcgc caattattct gggagcacga atgcaaagaa
     4321 tggttgtaca gaacagaacg ataataatca cgcatcacat tttgaggtaa acatcatgac
     4381 caatatcgaa agattagaac gctgtgccga tattatgcgg cgcagatgga tttatgaccc
     4441 tgaagcgggt ttattgttta gccgtgaaac aaaagacgtt atcaaaggca gcatgaccaa
     4501 taaagggcat ttggttgtaa cggtacatga aggtaaattc aaagtgacac tcacctatca
     4561 gaaagcctgc tatgtataca catacggcgc ttatgacgag acattatatg aagtgcacca
     4621 cgttaatttg aacaaacagg ataaccgcat caagaatatt cgcctgatgc cgaaagaaaa
     4681 gcaccgccgg atacataaaa acgtccgcaa acttattcgg ctgggccaca cacaatggct
     4741 tcaaccgcaa ttaattgaac aatcactata aacacaggag taaataccat gcattcatta
     4801 gacagttatt ttcagcgcac gacagcccct aaatcagccg cccaggaacg ccgtgaggaa
     4861 ttccatgaga aagtgatgcg ttctgctgat tatattgcgg acaagttcgt ggaaacggtt
     4921 cgtccacttg ttgatgaagt agctgataaa cttcagtctg aaatgccgga agatatggaa
     4981 ggcactgcaa agcggcgact catttgcgaa ttaagtcgtc gttttggcgt gtcaataagc
     5041 gcttttaaat aatcgaaaaa aggggggaaa gataatggac gcatttgagg tactggcaaa
     5101 taaaggcgct gaactggtag cacagcgtga taaaaccgcc aacgaagggg aacgcgcact
     5161 gctaaataaa cagattaaag cgatccgtat ggcccagttt aaattaatca gtaatgaagt
     5221 tatcgaaaca attaaaccgc agatcccaca aatcgttgct gacgctatta aagccgccgg
     5281 actggagaaa cgcattgccg ggctaaaaac gaacgcagag aaaatggaga tgcacaaaca
     5341 gagcggtgct ccgaatcccg atgagtactt ctcaccggat gttgagttta tggcgcaggt
     5401 tgaggaacgt ctgggtagct gtctcaccga ggaacagcgt cgctattttg atggagtgga
     5461 tagttcggca ggtattgacc tgaactcgta tttcggtcgt gaaattgagc attttgatgc
     5521 cgccccgtct atgccggagc cagaaccaga aatggccacc gatcctgaag ctgagcaacg
     5581 gaaacgcgta ttcaatgcga tagggattcg gtactgatcg gataaaagaa agccgggaat
     5641 taccccggct gacttaacaa tgtgaccaat gaacaaatcg gacaagacaa acaggataag
     5701 cagtctcgaa acaatgttcg ggaactatat tagcacaagt ccggagaaaa tcatcatgtt
     5761 ggcactacgc atcatgcagg gcatcgctaa gaccctcgca gaacatgtct tagacctgaa
     5821 acattcgcca ctgagcaaac aggccatgaa gcgccaaacg cttcgtctgt gggctgaata
     5881 ttcgcttgga actatcaata aaatcatcga catgaaatcg gggccgagca accagagcgc
     5941 ggaggaaatg gagttcatca ggcgtttaat tctcattcgc agagacattc atagccagtt
     6001 acacagcgtg ggaatcgata ttaatgatgg gacgggcgat taattttcgg acaagtacta
     6061 tgcgcgcgcg aggagctgaa catgaagcaa gatattgcag acaggctgga gatactcgaa
     6121 ggccagcggg cagaagcgaa acaactacgt aagcaggcca gaagagcgca caggaacaac
     6181 gaagcagagt tactaaccaa atacatcagt ttcaccaatt actgcatcta cgagtgttac
     6241 aaggaagatg cagaggactg gctggattca ttgccagaac agtactgagc agaataacac
     6301 cacgtcatgt tgttctgagg tgagcatatc acctctgttc aggtggccaa atttagtgtg
     6361 ccacttcagc atcaccgttg aaatcacaat aacgattttt ttgtaaatat gctctcacat
     6421 atgtaggaat agaccaggtt ggcattgaaa cacatctacc atgaccataa taaagtaatt
     6481 ttttgatatg tttatggttt tatattaatt aaaatatatc taaaattgat agttttaaaa
     6541 ttgcaaaatc cagattgtgt ttgtaatgtt taatctgatt gtattgagtg gcgtttatta
     6601 tgaaaaataa atcaggtaat tcttgttata gagaagcagt cgatattcat agctcacttg
     6661 agctttcatt tatattgcat aaatatcatt tgcgtgtaga tgagaagaaa tctcgaaatg
     6721 tattcagcgc catacgtgat gagctcatcc ttgatgggga ttcacgacaa aacttagcta
     6781 ctttctgtca aacatggctt gatgatgaaa tccgtgagtt gcttaattta gctattgata
     6841 aaaacatgat ttataaagat gaatatccac aaatggcgga acctgaaggt cgctgtgtgc
     6901 gtatgctttc cgattcgtgg agtttccccg attcgcgaca caccttaggt tgctcaacca
     6961 ttggttcatc tgaagctgct atgctcggcc gcttggcgtt aaaatggcag tggtgtaaaa
     7021 aacgcgaagt acaaggtaaa tcaaccgaac ctgatttgcg atcctgtgca aatatgctgg
     7081 cataaattct gccgttactg tgatgtgaaa ttgtgtgaaa ttccacttga tgatcatcac
     7141 ctgataatga atgctaaaga ggttttgaag catgtcgatg aaaacactat tggtgtcgtt
     7201 acgactttag gtattactta tttttgtaaa gcagattgcg ttagtgtttt taagataagt
     7261 gttaaagtta atattagttt ttatatttac atcgtaatta tatgtttcta ttttaaaaat
     7321 gttcgagagt ataaaaatgt ctaatgctaa tatcgcaccc aaaagtaaga aaatgacagt
     7381 ggggacactc gctatcatga atataactgc tgtagtaagt ctacgattta taccttctga
     7441 agcagaatac ggcttaggtg ccatttttta ctataccttt gctgcaatta tgtttttagt
     7501 acctgttgcc tttgttgcgg ctgagctagc tacaacatat ccacaaaaag ggggggcatt
     7561 tcgatggata ggagaagcat ttggggctcg ctggggtttt gtaggaatgc ttatgacctg
     7621 gctacaagtc atcccctact ttccaacagt actgacattt ggtgcagtct cggttgcatt
     7681 tattgatgtt gatatggggg tggcagaatc aattgccagt aataaatggt atattttatt
     7741 tttcgtacta tttgtttact ggggagctat ttgtgttgca ttacgtggcg ttggtatttt
     7801 ctcgcaggta tctaagtggt gcggtattat agggacaatt attccagcta ttatccttgt
     7861 tattcttggt ttctcctatc tgtggttcag cggtaaacca gcggtgatcc cattaggttg
     7921 gggtgatctt ttccctaata tagctaactt ccagaatatt gttcttgccg ccagcatatt
     7981 tcttgcttat gcagggatgg aaatgaatgc ggtccatgtt aacgatttag ataatccaac
     8041 taaaaaatac ccaattgcaa ttaccatcgc ttctataggt actattgcaa tattcctgct
     8101 cagtacttta ggggtcgcct ttattattcc tacagataaa attagtctaa ctcaaagttt
     8161 gttggttgcc tacgatatgc tgttcgaatg ggctggagtg ccatggcttg gctctgtgat
     8221 ggcatttatg ctagcaattg gcgtactggg tggggtggtt acttggattg cagggcctaa
     8281 tacaggtgta ttagcgatcg cgaaagctgg ttatttacct aagtttttcc aaaaaaccaa
     8341 tcgtcatgga atggggcacc atctaatgtt tgtacaaggt ataattgtct cagttctttc
     8401 tgttactttt gtgataatgc cttcagttca agcagctttt cagattttat cacagttaac
     8461 agttatgctc tatttagtta tgtatatgct catgttcgct agtgctatct atctacgaca
     8521 ttctcaacct gatacaaaac gcccatatcg agcccctgct ttatttttct gggcaacact
     8581 tggattctta gggtcttttc tggcttttgt attaagtttt attccgcccg agcaaatccc
     8641 aactggtagt ccaaccagct atgttcttta tttgttagta ttagttgccc tatttttatt
     8701 aattccaata ttaatttacg ccttccgaaa atctgactgg aaagatccaa actctgattt
     8761 cgaaccattt tcttgggaaa aaagaactag taattaaatt ttccataagc tacaaaagat
     8821 gaggttaaat atgtttagtg ctgaatctcc aattggtgta atagactcag gtgtaggtgg
     8881 attgacatcg gtaaaggaaa taattaattt gcttcctggt gaagatatta tctattgtgg
     8941 agataatatg aatgcacctt atggcaaccg ttctgccgat gatatcatca gtttgactaa
     9001 aaaaatgctg acttttttac agtcaaggaa tgtcaaacta gttgctgtcg catgtaatac
     9061 tatatcctca actttggaat ccgaagaata cagcggatat gctaagagct ttccgtttcc
     9121 tatcttatcc atcattgaac ctgcagtaga agatgtgatt cgccagcaat ataaaaatgt
     9181 tggtattatc gctactgagt ttactattaa aacggggtgt cacaaagagc taataaaaaa
     9241 attgaattca acaataaatg tatttggaga accaagcaaa aatcttgcaa tgttaattga
     9301 ggaaggtaat cttaatgctc ctgcaatctt gaatgacatc aaaaaacatg ttaaccattt
     9361 aatttccctc catccagtaa atgaaattat tctcggctgt actcactatc caattgttca
     9421 gaattttttt gaagcagttg caccggatat aaaatttatt aatcctgcac atgatcaggc
     9481 tatctctatt aaaaatcact tgggacagct aaatttatta aactcctcaa atataggaac
     9541 tttgcaaata aatactagtg gaagtatgga gatctacaaa acggtactag gggaattgag
     9601 cattacgaaa cctcatacat tcagtatcag gcagttttaa atttatccgg tgcaccgtaa
     9661 aatcccgtcc ttctgaactg caccccaaaa gttggacacc aactgagtaa ggtgcatttt
     9721 atggacaaac ccagttacat actgggtagt gcatccaatt agtagaacat gtgtttttcg
     9781 ataaacgctc cgatcacttt ttcgtggatc tcaactgagc gtgagaagca gattgtttta
     9841 cgacccaacc gcttaatgcg ggtgcgtagc gtcagattat tacgctcaat gcgttgggtg
     9901 aatattttgc cggtcagatg cttattcttc ggcacctccc ggccatagct gccccagtca
     9961 tcgctggtca gcatgccgat gttgaagggt gtaagcagtg ccagtagctc ccggcacgtt
    10021 tgatcggttc ggggaccaaa agtgtaggcc agtacaccgc ctgttttggt gttgtacgcg
    10081 tacccgagcc aatgttgccg ggctttactg ccaacgtagc tccattgctc atcaagctcg
    10141 cagataaacg ccacatcagc atgggcaaca ggcgaagaag ttattcgctt tggcgcgagt
    10201 tttttaaagt ccggatgacg gtgttactac caattttcag tgtcctggcg gtatcgcgaa
    10261 ccccggcacc attaaaggcc atttcagtta tcagctcttt catacccggc ttacgtgctt
    10321 gataagtata agtgaactga aacacacggt ggcagtcacg gcagcgaagt ctgtcacggc
    10381 ctttagggtt ctgaccatgg cggtaaacct gagctgactg acaacgggga caatgaatat
    10441 taacgctggt catgagataa cctcaaaagc ccgtattata catcagattc aactaattag
    10501 aggcatcacc catccagcat tataaaacat actaacaata actaaattat aataatcaca
    10561 acttacaatc aaataataaa tgcataagat taacaatgat tttttcgggt tatatttatc
    10621 taaaaaagca gaatattatg taacttatta ttttctaagc gaaaatttat tgtgcggtca
    10681 gactaacaaa cttgcgtacc acccgaagaa catgcgaaga tttaagtatt ctttcttttt
    10741 tggtttttat ttttcatttt ctcttttctt ctctttccgg ttctgttctg gttttggttt
    10801 tttccttttt atttttattc tttcttcagg gttatgctaa aacaccaaaa catttttcac
    10861 cacaaaagca tccaatcatc ttgccagacc aatcacctta accggataca ctgccggata
    10921 taatcccact aacacccaga caccaagcac catacctgat aactaccgta ttcaggaagg
    10981 aaatgcgagt gatttcaccg tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat
    11041 gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg ggagttgttg
    11101 tagctg
//
LOCUS       DAJTUN010000122        10954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTUN010000122 DAJTUN010000000
VERSION     DAJTUN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.122"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(207..282)
                     /locus_tag="OO470_003225"
     tRNA            complement(207..282)
                     /locus_tag="OO470_003225"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(247..249),aa:Ile,seq:cat)
     gene            841..3093
                     /locus_tag="OO470_003226"
     CDS             841..3093
                     /locus_tag="OO470_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252380.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3429..4406
                     /gene="csiD"
                     /locus_tag="OO470_003227"
     CDS             3429..4406
                     /gene="csiD"
                     /locus_tag="OO470_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6252381.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4426..5694
                     /gene="lhgO"
                     /locus_tag="OO470_003228"
     CDS             4426..5694
                     /gene="lhgO"
                     /locus_tag="OO470_003228"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6252382.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5717..7165
                     /gene="gabD"
                     /locus_tag="OO470_003229"
                     /pseudo
     CDS             5717..7165
                     /gene="gabD"
                     /locus_tag="OO470_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7173..8459
                     /gene="gabT"
                     /locus_tag="OO470_003230"
     CDS             7173..8459
                     /gene="gabT"
                     /locus_tag="OO470_003230"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6252383.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8697..10097
                     /gene="gabP"
                     /locus_tag="OO470_003231"
     CDS             8697..10097
                     /gene="gabP"
                     /locus_tag="OO470_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6252384.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10118..10780
                     /gene="csiR"
                     /locus_tag="OO470_003232"
     CDS             10118..10780
                     /gene="csiR"
                     /locus_tag="OO470_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6252385.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10781..>10954)
                     /gene="kbp"
                     /locus_tag="OO470_003233"
     CDS             complement(10781..>10954)
                     /gene="kbp"
                     /locus_tag="OO470_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6252386.1"
                     /translation="ATASQFYTVKSGDTLSAISKQVYGNANLYNKIFEANKPMLKSPD
                     KIYPGQVLRIPEE"
BASE COUNT         2816 a         2674 c         2752 g         2712 t
ORIGIN      
        1 tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat aaatacattg
       61 cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac cgcacttctt
      121 ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt gtacccttaa
      181 ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac cagcgaccaa
      241 gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat gcaataacaa
      301 tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag atttttatgc
      361 gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc gcctccattc
      421 gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat ctatagttat
      481 catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt tattacatta
      541 gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa aaataaactt
      601 catatagcaa tatattcttt catagatctt attctgctaa tcattagttt cgtatgcgcg
      661 atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt taaacattat
      721 ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac tttcacaaca
      781 attaaacatt tttatgtttt cgtatacatc atattactct accattagag gaacgttatt
      841 atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac attgttatca
      901 tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata tctaaactgt
      961 ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga cagagaaatt
     1021 atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga ttataaaatt
     1081 tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag tagcattaca
     1141 gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac aatggttgaa
     1201 agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac tcacattata
     1261 tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat tcctgactat
     1321 gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca gcaagaaaca
     1381 tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa atctcttact
     1441 gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga agcgattctt
     1501 catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt tgcacgttcc
     1561 cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg accagaaact
     1621 cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct ttgtaccatc
     1681 cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc taaaaaattt
     1741 ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg gagtggtaaa
     1801 aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga tatagaaagt
     1861 ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga tccagtaaca
     1921 aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga cttcgaagca
     1981 agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa agaaaagagt
     2041 ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt gcaaaatcaa
     2101 tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc agcaaaacac
     2161 tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta taatgagcga
     2221 ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat gaattacatg
     2281 gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct ttatgaaaga
     2341 gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc cttttcattc
     2401 gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag catcccatca
     2461 gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa tcttttcaat
     2521 gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat ggccgctctc
     2581 ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac gactgaaatt
     2641 aaaaattatg atgggtccac gcgtgtcggt ggtgaggcgt ggcttaataa aggttgctct
     2701 acctatcagc agctctacaa tgaattccac gcattatata tagataaagc aggaatatgg
     2761 agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg tggggattct
     2821 gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg aaacgaagaa
     2881 gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat ggggagtaat
     2941 agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa agtatttacg
     3001 cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct tttagggaaa
     3061 acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc cccgataaaa
     3121 cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc atttactgtt
     3181 ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt tgctttagtt
     3241 attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta atttgttgct
     3301 tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga aatgtagata
     3361 tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc ataagaggat
     3421 cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca ggccaggact
     3481 atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca
     3541 ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg caggcgctgg
     3601 agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg tgtgcgaatc
     3661 agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg ctgttgatca
     3721 atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg gcaacggcgg
     3781 tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat tacgcgcgtt
     3841 tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac cgcgtgatgg
     3901 aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg atgatgaaaa
     3961 tcgatgagca aaacatgcag ggtggaaatt cgttgctgct gcatctcgat gactgggaac
     4021 atctggacca ctatttccgc catccactgg cgcgtcgccc gatgcgcttt gccgcgccgc
     4081 cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat caacagggtc
     4141 gcccggtgat gcgctatatc gaccagttcg tccagccaaa agattttgaa gaaggcgtgt
     4201 ggttgagcga gctttccgat gccattgaaa ccagcaaagg cattctttct gtacccgttc
     4261 ccgttggcaa attcctgttg ataaacaacc tgttctggct gcacggtcgc gaccgcttta
     4321 ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc gcttacgcca
     4381 ctcaccacta ccagacgcat cagtaagcgc aaaggaattg agcggatgta tgattttgtg
     4441 attattggcg gcggcatcat cggcatgtcg accgccatgc aactgattga tgtttatccg
     4501 gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca gacgggccac
     4561 aacagcggcg tgatccatgc cggggtctat tacacgcccg gcagcctgaa ggcgcagttt
     4621 tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc aaaacggcat tcgctatgac
     4681 aactgcggca agatgctggt cgccacgtct cagctcgaaa tggaacggat gcgcgcctta
     4741 tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga taaacgccgt ggaactgcgc
     4801 gagcgcgaac cgaatatcac cgggcttggt ggcatttttg tgccgtccag cggcattgtc
     4861 agctaccgcg aagtgacggc ggcaatggca aaaatcttcc aggccagagg cggcgagatt
     4921 atttataacg ccgaagtcag cgccctcagt gagcataaaa acggcgtggt gatacgcacc
     4981 cgtcagggcg gtgaatatga agcatcgacg ctgattagct gttccgggct gatggctgac
     5041 cggctggtga aaatgctcgg cctcgaaccg ggctttatta tctgcccgtt ccgtggcgag
     5101 tatttccgcc ttgcgccgga gcataaccag attgttaacc acctgattta ccccattccc
     5161 gaccccgcga tgccattttt gggcgtgcat ctcacccgca tgatcgacgg cagcgtgacc
     5221 gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg cgacttctca
     5281 tttagtgaca cgctggagat tttgagctcg tcggggattc gccgggtgct gcaaaaccat
     5341 ctacgctcag gactgggcga gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg
     5401 gtgcaaaagt attgtccccg gctttcgtta agcgatctcc agccctggcc cgccggtgtg
     5461 cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg attttctgtt tgtcaccacc
     5521 ccacgcacga tccacacctg caatgcgccc tccccggcag cgacatcagc aattcctatt
     5581 ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc
     5641 acgctgcgag cggcacgtag tgtggatgcc ttacacgccg catttaatca ataacctttg
     5701 aaaacaggac gtaacaatga aacttaacga cagtaactta ttccgccagc aggcgttgat
     5761 taacggggaa tggctggacg ccaacaatgg tgaagtcatc gacgtcacca atccggcgaa
     5821 cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat gaaacccgcg ccgctatcga
     5881 cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc gccaaagaac gcgccaccat
     5941 tctgcgcaac tggttcaatt tgatgatgct gcatcaggac gatttagcgc gcctgatgac
     6001 cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa atcagctacg ccgcctcctt
     6061 cattgagtgg tttgctgaag aaggcaaacg tatttatggc tacaccattc ctggtcatca
     6121 ggccgataag cgcctgattg ttatcaagca gccgattggc gtcaccgccg ccatcacgcc
     6181 gtggaacttt ccggcggcga tgattacccg taaagccggt ccggcgctgg cggcaggctg
     6241 caccatggtg ctgaagcccg ccagtcagac gccgttctct gcgctggcgc tggcggaact
     6301 ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt cggcaggcgc
     6361 ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa ctgtcgttta ccggttagac
     6421 cgagattggc cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga
     6481 gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata aagccgtgga
     6541 aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt
     6601 atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa ttacagcagg cggtgagcaa
     6661 actgcacatc ggcgacgggc tggagaaagg cgtcaccatc gggccgctga tcgatgaaaa
     6721 agcggtagca aaagtggaag agcatattgc cgatgcgctg gagaaaggtg cgcgtgtggt
     6781 ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc cagccaacca ttctggtgga
     6841 cgttccggcc aacgccaaag tgtcgaaaga agagacgttc ggccccctcg ccccactgtt
     6901 ccgttttaaa gatgaagccg atgtgattgc acaagccaat gacaccgagt ttggtcttgc
     6961 cgcctatttc tacgcccgtg atttaagccg cgtcttccgc gtcggcgagg cgctggagta
     7021 cggcatcgtc ggcatcaata ccggcattat ttccaatgaa gtggccccat tcggcggcat
     7081 caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt acttagaaat
     7141 caaatatatg tgcatcggtc tttaactgga gaatgcgaag gaacagcaat aaagagttaa
     7201 tgcagcgccg cagtcaggcg attccccgtg gcgttgggca aattcacccg attttcgctg
     7261 accgcgcgga aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt gatttcgcgg
     7321 gcgggattgc ggtgctcaat accgggcacc tgcatccgaa ggtggtggcc gcggtggaag
     7381 cgcagttgaa aaaactgtcg cacacctgct tccaggtgct ggcttacgag ccgtatctgg
     7441 agctgtgcga gattatgaat cagaaggtgc cgggcgattt cgccaagaaa acgctgctgg
     7501 ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat cgcccgcgcc gccaccaaac
     7561 gtagcggcac catcgctttt agcggcgagt atcacgggcg cacgcattac accctggcgc
     7621 tgaccggcaa ggtgaatccg tactctgcgg gcatggggct gatgccgggt catgtttatc
     7681 gcgcgcttta tccttgcccg ctgcacggca taagcgagga tgacgctatc gccagcatcc
     7741 accggatctt caaaaatgat gccgcgccgg aagatatcgc cgccatcatg attgagccgg
     7801 ttcagggcga aggcggtttc tacgccgcgt cgccagcctt tatgcagcgt ttacgcgctc
     7861 tgtgtgacga gcacgggatc atgctgattg ccgatgaagt gcagagcggc gcggggcgta
     7921 ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc ggatcttacc acctttgcga
     7981 aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg gcgcgcggaa gtgatggacg
     8041 ctgttgcacc gggcggtctg ggcggcacct atgccggtaa cccgattgcc tgcgtggctg
     8101 cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct gcaaaaagcc aacgatctgg
     8161 ggcagaagct aaaagacgga ttgttggcga tagccgaaaa acacccggag atcggcgacg
     8221 tacgcgggct gggatcgatg attgccatcg agctgtttga agatggcgat cacagcaaac
     8281 cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg cgataagggc ctgattcttc
     8341 tctcctgcgg cccgtattac aacgtgctgc gcatccttgt accactcacc attgaagacg
     8401 ctcagatccg tcagggtctg gagattatcg gtcagtgttt tgatgaggcg aagcagtagc
     8461 accgccccca tgcaggaggc gacgctgcgc gtcttgtccg gcctacgaga atccaagtcg
     8521 gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa caggaataaa tctatgtcgg
     8581 ccggataagg cggagccgct ctccggcaaa aagaatcaat aacaattaaa cgcgtgaccc
     8641 gacgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     8701 ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     8761 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     8821 aagcggaccc ggcggtatta ctggcctatc tgttcgccgg attactggtg gttatgatta
     8881 tgcggatgct ggcggaaatg gcagttgcta cgcccgatac cggttcgttt tccacctatg
     8941 ccgataaagc cattggccgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     9001 gggtactggt tatcccactg gaagccaaca tcgccgccat gatcctgcac tcgtgggttc
     9061 caggcattcc catctggtta ttttccctcg tcattaccct cgccttaact ggcagtaatt
     9121 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     9181 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     9241 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     9301 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     9361 ttgccgccgc ggaatccgac atgccggaaa aacatattgt ccgcgccacc aactcggtta
     9421 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     9481 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     9541 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     9601 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     9661 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     9721 ccggcgcagc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     9781 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     9841 agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga aattcgcttg cgaatgtggc
     9901 ttgatccctg gctcacctgg ctggtcatca gctttattac ctttgtgttg gtagtgatgc
     9961 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
    10021 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
    10081 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
    10141 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
    10201 gatgagttta ctgacatcgc gttatgcact tggcgttggg ccgttgcagg aagcactttc
    10261 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
    10321 tatgtcagag caggagctgc tcgatatttt cgacgcccgc gccaatatgg aagcgatgct
    10381 ggtcagtctg gcgattgccc gcggtggcga tgaatgggag gcagacgttc tcgcaaaagc
    10441 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
    10501 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
    10561 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat cgatttatct ggctgcggcg
    10621 aacggtgctt tcggtggaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
    10681 ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta atgcgccagc atttactgac
    10741 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
    10801 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
    10861 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
    10921 cagacttaac ggtataaaac tggctggcag tggc
//
LOCUS       DAJTUN010000123        10797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTUN010000123 DAJTUN010000000
VERSION     DAJTUN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.123"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1282
                     /locus_tag="OO470_003234"
     CDS             <1..1282
                     /locus_tag="OO470_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6252387.1"
                     /translation="SRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNHGYYT
                     AAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYY
                     NSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAP
                     DQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDG
                     PLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISI
                     PKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDD
                     YPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEM
                     QGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1334..2491
                     /locus_tag="OO470_003235"
     CDS             1334..2491
                     /locus_tag="OO470_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6252388.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2457..2579)
                     /locus_tag="OO470_003236"
     CDS             complement(2457..2579)
                     /locus_tag="OO470_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252389.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2782..4467
                     /locus_tag="OO470_003237"
                     /pseudo
     CDS             2782..4467
                     /locus_tag="OO470_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4505..6865
                     /locus_tag="OO470_003238"
     CDS             4505..6865
                     /locus_tag="OO470_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6252390.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQETRKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6922..9705
                     /locus_tag="OO470_003239"
     CDS             6922..9705
                     /locus_tag="OO470_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6252391.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10066..>10797
                     /locus_tag="OO470_003240"
     CDS             10066..>10797
                     /locus_tag="OO470_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6252392.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDA"
BASE COUNT         3164 a         2407 c         2365 g         2861 t
ORIGIN      
        1 ctcccgtgcc gcaataatga ccggtcgagc tcccgcccgc tttggtgtct attccaatac
       61 cgatgctcag gatggtattc cgctaacaga aactttcttg cctgaattat tccagaatca
      121 tggttattac actgcagcag taggtaaatg gcacttgtca aaaatcagta atgtgccggt
      181 accggaagat aaacaaacgc gtgactatca tgacaacttc accacatttt ctgcggaaga
      241 atggcaacct caaaaccgtg gctttgatta ctttatggga ttccacgctg caggaacggc
      301 atattacaac tccccttcac tgttcaaaaa tcgtgaacgt gtccccgcaa aaggttatat
      361 cagcgatcag ttaaccgatg aggcaattgg cgttgttgat cgtgccaaaa cacttgacca
      421 gccttttatg ctttacctgg cttataatgc tccgcaccta ccaaatgata atcctgcacc
      481 ggatcaatat cagaagcaat ttaataccgg tagtcaaaca gcagataact actacgcttc
      541 cgtttattct gttgatcagg gtgtaaaacg cattctcgaa caactgaaga aaaacggaca
      601 gtatgacaat acaattattc tctttacctc cgataatggt gcggttatcg atggtcctct
      661 gccgctgaac ggggcgcaaa aaggctataa gagtcagacc tatcctggcg gtactcacac
      721 cccaatgttt atgtggtgga aaggaaaact tcaacccggt aattatgaca agctgatttc
      781 cgcaatggat ttctacccaa cagctcttga tgcagccgat atcagcattc caaaagacct
      841 taagctggat ggtgttccct tgctgccctg gttgcaagat aagaaacaag gcgagccaca
      901 taaaaatctg acctggataa cctcttattc tcactggttt gatgaggaaa atattccatt
      961 ctgggataat taccacaaat ttgttcgcca tcagtcagac gattacccgc ataaccccaa
     1021 cactgaggac ttaagccaat tctcttatac ggtgagaaat aacgattatt cgcttgtcta
     1081 tacagtagaa aacaatcagt taggtctcta caaactgacg gatctacagc aaaaagataa
     1141 ccttgccgcc gccaatccgc aggtcgttaa agagatgcaa ggcgtggtaa gagagtttat
     1201 cgacagcagc cagccaccgc ttagcgaggt aaatcaggag aagtttaaca atatcaagaa
     1261 agcactaagc gaagcgaaat aactaaacct tcatgcggcg gatttttccg ccgccttatt
     1321 gagcgagata gcgatgcacg ttacagccaa gccctccagt tttcaatgta atctcaaatg
     1381 tgattactgt ttttaccttg aaaaagagtc gcagtttact catgaaaaat ggatggatga
     1441 cagcactctg aaagagttca tcaaacaata tatcgaagcg tctggcaatc aggtctattt
     1501 tacctggcaa ggcggtgaac ccactctggc tggcctggat tttttccgta aagttattca
     1561 ctatcaacaa cgctatgcag gccaaaaacg tatttttaat gcattacaaa cgaatggcat
     1621 tttattgaat aatgaatggt gtgcctttct caaagaacat gaatttctgg ttggtatctc
     1681 gatcgatggc ccccaggagt tacatgaccg ttacagacgc agtaattcag gtaacggtac
     1741 ttttgcaaaa gtgatagcag ccatcgagcg tctgaaatca tatcaagtag agtttaatac
     1801 gttaaccgtc attaataacg ttaatgtcca ttatcctctt gaggtttatc attttttaaa
     1861 atctatcggc agtaaacata tgcaatttat cgaattgcta gaaaccggga cgccgaatat
     1921 tgatttcagt ggtcatagtg agaacacatt ccgtatcatt gatttttctg tgcctcccac
     1981 ggcttatggc aagtttatgt caaccatttt tatgcaatgg gttaaaaacg atgtgggtga
     2041 aattttcatt cgtcagtttg aaagctttgt cagccgtttt ttggggaatg ggcatacaag
     2101 ttgtattttc caggagtcct gcaaggataa tctggttgtt gaaagtaatg gagacattta
     2161 cgaatgcgac cattttgtct atccacagta caaaattgga aacattaata aatctgaact
     2221 caaaacgatg aacagtgtac aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca
     2281 gcaatgtgca tataaaccta tctgcaatgg cggttgtcct aagcatcgta ttactaaagt
     2341 aaacaatgag actgtttctt atttttgcga aggttataaa atcctttttt caaccatggt
     2401 accttatatg aacgccatgg ttgagttagc taagaacaga gtaccgcttt accacattat
     2461 ggatgttgca aaacaaatgg agaataatta ataacgctct cttttaactc cattcttagc
     2521 ctccttctta tgaccttaac aaggcacccg acataaattc ctgtcgggtg tttcgtcatc
     2581 atcatcttcc cttgcgaaaa tcctggctta cgtggtatgc gtcagggcat tagcaatacc
     2641 cttgattatc aatagcataa ccataaccac tgaaaattaa aaatttctat atatttcata
     2701 ttgttaaatg atgcttaaat aaatttcatc agtaaatcat tgccatagat aatcattgga
     2761 tggcacaatc aattttcgct aatgataacc attcccatta cgttttgtat gttgatagca
     2821 aagtatctct gtctgctgaa gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg
     2881 ttaatcatta ttatcctcgc aatgatcctt ggcgtggtta aaattcaggt ctggttaaac
     2941 gactggaata atgatttttt caatgcgcta agccagaaag aaaccgataa gctttggcag
     3001 ctcgttcttt ggttccctgc cctgctgggg atctttgtat taatatccgt aaacaaaaca
     3061 tggttaatta aactactgac tattcgctgg cgcgaatggt taacagatta ctaccttaat
     3121 cgctggtttg cagataaaaa ctactacttc acgcagatct acggcgagca taaaaataca
     3181 gataaccctg accaacgtat cgctgaagac attctcttac tgatcaacaa aacgctcagt
     3241 ctttcatttg gcttcatcca gtcacttagc atgctgatca cctttaccgt tattctttgg
     3301 caaagcgcgg gtacgctctc attcactgtt ggtggaacag aatggaatat ccaggggtat
     3361 atggtctata ccgtcgtgct cattgtgatc ggtggaactt tatttaccca taaggtgggt
     3421 aaacgtattc gtccgcttaa tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat
     3481 cttgtgcagc ataataagca ggcagaactg attgcgctaa gcaatgctga gtctctacaa
     3541 cgccaggagt tgcgcgacaa ttttcatacc attaaagaga actggcatcg tttaatgaat
     3601 cgtcagcggt ggcttgatta ctggcaaaat atttattcgc gctcgcttag cgttcttccc
     3661 tactttctgt tattgccgca gtttattagc gggcagatca atctgggcgg actgatgaaa
     3721 tcgcgccagg catttatgct agtatcgaac aatttaagct ggtttattta taaatatgac
     3781 gaacttgctg aactggctgc ggttatcgat cgcttgtatg agttccatca actcactgaa
     3841 tagcgcccta cgaataagcc taaaaattgc caacatgcgg tacaagtggc taatgcgagt
     3901 attcgtacgc ctgataataa gatcatatta gagaacttga actttcatgt ttcgccaggc
     3961 aaatggctat tactgaaagg ctactctggt gcgggaaaaa ccacactgct taaaacatta
     4021 tcccactgct ggccgtggtt taaaggtgat atttcttctc ctgctgacag ttggtatgtg
     4081 tcacaaacac cgttaatcaa aagcggctta ctgaaagaga ttatttgtaa agcacttccc
     4141 ctgcccgtag acgataaatc gttgagcgaa gtactgcatc aggttggtct gggaaaattg
     4201 gctgcgcgta ttcatgacca cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa
     4261 cgtatcgccc tggcacgatt aattttacga cgtccgaaat ggatatttct tgacgaaact
     4321 acctctcatc ttgaggaaca agaggctata cgcttactgc gtttagtgcg tgaaaaacta
     4381 cccacaagcg gcgtcattat ggttacacat caacccggtg tctggaacct ggctgatgat
     4441 atttgtgaca ttagcacggt tttataacaa caataaaagc cctgctgtac ggagatataa
     4501 ataaatgaag cgagttctta ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc
     4561 cgtcgatgac aaaaacatgt acatgtattt ttttgaagag atgacggtct atgctcctgt
     4621 ccctgtaccc gtaaacggca acacgcatta caccagtgaa agcatcgagc gtttaccgac
     4681 cgggaatggc aatatcagcg atctgctgag aaccaaccct gcggtacgca tggattcaac
     4741 gcaaagtacc tcgttgaacc agggagatat tcgccctgag aaaatctcta ttcacggtgc
     4801 gtcgccctac cagaatgcct atttgattga cggtattagt gcaactaata acctgaaccc
     4861 agcgaatgag tccgatgcca gtagtgcaac caatattagc gggatgtcac aggggtatta
     4921 tcttgatgtc agcttactgg acaatgtgac gctttatgac agttttgtgc cggttgaatt
     4981 tggtcgcttc aatggcgggg taattgatgc aaagatcaaa cgcttcaacg ctgatgatag
     5041 caaggtgaaa ttgggttatc gcactacgcg tttggactgg ttaacatcgc atatcgatga
     5101 gaataacaag agcgcattta atcaaggttc ttcaggaagt acctatttct ctccagattt
     5161 taaaaagaac ttttatacct tgtcgtttaa tcaggagctg gctgataact ttggcgttac
     5221 cgccggttta tcgcgccgcc agtctgatat cacccgcgcg gattatgttt cgaatgacgg
     5281 cattgtcgcc ggtcgggcac agtataaaaa cgttatcgat actgcattga gcaaatttac
     5341 ctggtttgcc agcgaccgct ttacccacga tttaacctta aaatataccg gctccagccg
     5401 tgattataat accagcacct tcccgcagtc tgatcgcgaa atgggtaata aatcctatgg
     5461 tctggcatgg gatatggata cgcaactcgc atgggccaaa ctacgtacca ccgttggttg
     5521 ggatcatatt agtgattata cccgtcacga tcatgacatc tggtacaccg aactttcatg
     5581 tacatatggt gatattacag ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc
     5641 tgtagataat tacaccttca aaacacgcct ggactggcaa aaattcgccg tgggtgatgt
     5701 ttcgcatcaa ccctacttcg gcgcggaata catctattcc gatgcatgga ctgaacgcca
     5761 taaccagtct gaatcctatg tgattaatgc tgcaggaaag aaaactaacc ataccattta
     5821 ccataaaggt aaaggcagcc tgggaattga caactacacg ctgtatatgg cggatcacat
     5881 tagctggcgg aatgtgtcgt taatgcccgg tgtgcgttat gactatgaca actatctgtc
     5941 aaaccacaat atctccccgc gctttatgac ggaatgggat atttttgctg atcaaacctc
     6001 aatgattacc gccggttata accgttacta tggcgggaat attcttgata tgggattacg
     6061 tgatatccgc aatagctgga cggaatcggt atcaggtaat aaaaccctga cgcgttatca
     6121 gaatttgaaa acgccttata acgatgaact ggcaatggga ttgcagcaaa aaatcgataa
     6181 gaacgttatt gcacgcgcaa gtgaagcgca tgatcaaatc agcaaaagca gtcgtaccga
     6241 cagcgcgact aaaaccacca ttactgaata taacaacgat ggcaaaacca aaacgcattc
     6301 gtttaacctc agttttgaac tggccgaacc cctgcatatc cgccaggtag atattaaccc
     6361 gcaaattgtc tttagctata tcaagagcaa gggcaacttg tcgttaaaca atggttatga
     6421 ggagagcaat accggtgata accaggtggt ttataacggt aatctggtct cttacgatag
     6481 cgttccagtg gcagatttta ataacccatt aaagatctcc ttaaacatgg atttcacgca
     6541 tcaaccgagc gggttagtgt gggcgaatac gctggcctgg caagaaacgc gtaaagctcg
     6601 cattatcctt ggtaagacaa atgcgcaata catcagcgaa tattcagatt acaagcagta
     6661 tgttgacgaa aaactggata gcagcctgac ctgggacacc cgcttgtcct ggacgccaca
     6721 atttctgaaa caacaaaacc tgacgatcag tgccgatatt ctcaatgtac tggatagcaa
     6781 aaccgctgtt gatacaacga acaccggtgt ggcgacctac gccagtggcc gtactttctg
     6841 gcttgatgtc agcatgaaat tttaatagtt cggattttac ttaaccgggt aaattcgccc
     6901 ggttttcgca tggagataat tatgagaaac ctctgtttct tactgacgtt agtggcaact
     6961 ctgttgctcc ccgggcggct gattgccgcc gccttaccgc aggatgaaaa gttaattacc
     7021 gggcaactgg acaatgactt gcgatatatg atttatccgc atgctcatcc aaaggatcag
     7081 gtaaatttat ggctgcaaat tcataccggt tcattgcagg aagaagacaa tgagctcggc
     7141 gtggctcatt ttgtagaaca tatgatgttt aacggcacaa aaacatggcc gggtaataaa
     7201 gtcatcgaaa catttgagtc aatgggcctg cgttttggtc gcgatgttaa tgcctatatc
     7261 agctatgacg aaacggtgta tcaggtgagt ttgccgacta cgcagaaaca aaatctgcaa
     7321 caagtgatgg caatcttcag tgaatggagt aatgccgcaa cctttgaaaa actcgaagta
     7381 gacgctgaac gtggcgtaat tactgaggaa tggcgtgccc atcaggatgc gaaatggcgc
     7441 acctctcagg cgcgccgccc tttcctgctg gctaataccc gtaatttaga ccgtgaacct
     7501 atcggcctga tggatactgt cgccacggtc acgccggcac aattgcgcca attttatcaa
     7561 cgctggtatc aaccaaataa tatgaccttt atcgtggtcg gcgatatcga cagtaaagaa
     7621 gcgctggcgc tgataaagga taatttaagt aagcttccgg ctaacaaagc agctgaaaat
     7681 cgcgtctggc cgacaaaagc cgaaaaccac ctgcgcttta atatcattaa tgataaagaa
     7741 aaccgggtga acggcatcgc actctattat cgcctgccaa tggtacaagt gaacgatgag
     7801 caaagctttg tcgaacaagc tgaatggagc atgttagttc agctgttcaa tcaacgtctg
     7861 caggaacgca tacagtcggg cgagttgaag actatttctg gcggcactgc gcgcagcgtt
     7921 aaaattgcac ccgattatca gtcgctgttt ttccgtgtaa atgcacgaga cgataatatg
     7981 caggatgctg cgaatgcatt aatggcagag ttggcaacca ttgatcagca tggtttttct
     8041 gctgaagaac tcgatgatgt caaatctacc cgcctcacct ggctgaaaaa tgcggttgat
     8101 cagcaagctg aacgtgattt acgtatgctg accagtcgcc tggcatccag ctcattaaat
     8161 aatacgccgt tcttgtcgcc ggaagagaca tatcaacttt cgaaacgtct gtggcagcaa
     8221 attaccgtgc aaagtctggc ggaaaaatgg cagcagttaa gaaagaacca ggacgcattt
     8281 tgggagcaaa tggtaaacaa tgagcttgcc gccaaaaaag cattgtctcc tgcggctatc
     8341 ctggcgctgg aaaaagagta cgccaacaaa aagctggcgg cttacatctt cccaggcaga
     8401 aatttatcgt taacagtaga cgctgaccca caggcggaaa ttagcagcaa agaaacgctg
     8461 gctgagaatc tgacatcatt aacactttcc aatggtgcca aggttattct ggcaaaatcc
     8521 gcgggtgaag agcaaaagct acaaattatt gccgtatcga ataaaggcga tttaagtttc
     8581 cctgcgcagc aaaaatcact tatcgcgctg gcaaataaag cagttagcgg aagcggcgtt
     8641 ggcgaactct cctcttccag cctgaaacgc tggagtgcgg aaaactcggt aaccatgagc
     8701 agtaaagtca gtggcatgaa tacgttgctc tccgttagcg cgcggactaa taaccctgaa
     8761 cctggtttcc agttgattaa ccagcgaatc acccacagca cgattaacga caatatttgg
     8821 gcatcgctac aaaatgctca aattcaggcg ttgaaaacgc tcgaccagcg tccagcggag
     8881 aaattcgccc agctgatgta tgagacgcgc tatgctgatg accgcacgaa attactgcaa
     8941 gaaaatcaga ttgcacagtt tactgccgca gatgcgctgg ctgccgatcg ccagttgttt
     9001 tcatctccag cggatatcac gtttgtcatt gtcggtaatg tcgcagaaga caaactcgtg
     9061 gcgttaatta cgcgttactt aggctcaatc aaacactctg attcgccatt agccgcaggt
     9121 aaaccattaa ctcgcgcgac ggacaacgca tcggttactg taaaagaaca aaatgaacct
     9181 gtggcacagg tttcacagtg gaagcgttat gattcccgga cacctgttaa tctggcgacg
     9241 cgtatggcgc tcgatgcttt taacgtcgca ctggcaaaag atctacgcgt taatattcgt
     9301 gaacaggcat ctggggcata cagcgtttct tctcgcctct cggttgatcc tcaggctaaa
     9361 gatatcagtc atttgctggc ttttacttgt caaccagaac gacatgatga acagttaacg
     9421 ttagcgaatg aagtgatggt taagcgtttg actaagggga tcagtgagca agaactgaac
     9481 gaataccagc aaaacgttca gcgcagcctc gatatccaac agcgtagcgt tcaacaatta
     9541 gcgaacacta ttataaatag tcttattcaa tatgacgatc ctgcagcatg gactgagcag
     9601 gagcaattgt tgaaacaaat gacggtagag aatgttaaca ctgccgttaa acaatatctt
     9661 tctcatccgg taaatactta taccggagta ttattgccaa aataataaca gccccgtcaa
     9721 cacatcgttg gcggggattt tagcaatatt cgctattttt atgtaataat tttataaatg
     9781 cgttcaaaat aataaccaag tactaatagt gatattttaa tgcctgattt ttacgtgata
     9841 attcaggagg cacagaaagc gcataaaaat aacaacataa aacacctttc caccacccaa
     9901 gaattctcta ttacattgtt tttaatgaaa aaatatcatt ccaacaatat ctcacgataa
     9961 ataacattag gattttgtta tttaaacacg agtcctttgc acttgcttac tttatcgata
    10021 aatcctactt ttttaatgcg atccaatcat tttaaggagt ttaaaatgga taagaagcaa
    10081 gtaacggatt taaggtcgga actactcgat tcacgttttg gtgcgaagtc tatttccact
    10141 atcgcagaat caaaacgttt tccgctgcac gaaatgcgcg acgatgtcgc atttcagatt
    10201 atcaatgatg aattatatct tgatggcaac gctcgtcaga acctggccac tttctgccag
    10261 acctgggacg acgaaaacgt ccacaagttg atggatttat ccattaacaa aaactggatc
    10321 gacaaagaag aatatccgca atccgcagcc atcgacctgc gttgcgtaaa catggttgcc
    10381 gatctgtggc atgcgcctgc gccgaaaaat ggtcaggccg ttggcaccaa caccattggt
    10441 tcttccgagg cctgtatgct cggcgggatg gcgatgaaat ggcgttggcg caagcgtatg
    10501 gaagctgcag gcaaaccaac ggataaacca aacctggtgt gcggtccggt gcaaatctgc
    10561 tggcataaat tcgcccgcta ctgggatgtg gagctgcgtg agatccctat gcgccccggt
    10621 cagttgttta tggacccgaa acgcatgatt gaagcctgcg acgaaaatac catcggcgtg
    10681 gtgccgactt tcggcgtgac ctacaccggt aactatgagt tcccgcaacc gctgcacgat
    10741 gcactggata aattccaggc cgacaccggt atcgacatcg acatgcacat cgacgcc
//
LOCUS       DAJTUN010000124        10731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTUN010000124 DAJTUN010000000
VERSION     DAJTUN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.124"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO470_003241"
     CDS             complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO470_003241"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6252393.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO470_003242"
     CDS             complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO470_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6252394.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4257..5057
                     /gene="nagB"
                     /locus_tag="OO470_003243"
     CDS             4257..5057
                     /gene="nagB"
                     /locus_tag="OO470_003243"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6252395.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5117..6265
                     /gene="nagA"
                     /locus_tag="OO470_003244"
     CDS             5117..6265
                     /gene="nagA"
                     /locus_tag="OO470_003244"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6252396.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6274..7494
                     /gene="nagC"
                     /locus_tag="OO470_003245"
                     /gene_synonym="nagR"
     CDS             6274..7494
                     /gene="nagC"
                     /locus_tag="OO470_003245"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6252397.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7542..8294
                     /gene="nagD"
                     /locus_tag="OO470_003246"
     CDS             7542..8294
                     /gene="nagD"
                     /locus_tag="OO470_003246"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6252398.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8691..10355
                     /gene="asnB"
                     /locus_tag="OO470_003247"
     CDS             8691..10355
                     /gene="asnB"
                     /locus_tag="OO470_003247"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6252399.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         2677 a         2772 c         2710 g         2572 t
ORIGIN      
        1 atgtaggcct gataagcgaa gcgcatcagg caatttagcg tttgaaattt attgtctatt
       61 tattgccgga tgcgacgtaa atgcctcacc cggcttggta gaacttaaaa ttactcgcct
      121 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttcggccgtg
      181 gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      241 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      301 atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag acgatcatac
      361 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      421 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      481 gtgatattac cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc
      541 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ctcgcggaaa
      601 tcggcgcgat cgatccaaat ttcaccgcta aacggcacct gacggctccc catttccggt
      661 ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc
      721 agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg
      781 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      841 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc
      901 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta
      961 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcggatga
     1021 acaggaatgg tgatgttatc cagtacccag tcgtacagac gacggttgtc ctggaactca
     1081 agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg
     1141 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1201 tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg
     1261 caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc caagttctct
     1321 tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt accgcggtat
     1381 tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1441 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1501 tgaaaaccta accactctac gtcgtttttg atcgaatcga catactcgat atcttctttt
     1561 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1621 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1681 aaacgggtgt gtactgtgat gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1741 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1801 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat
     1861 tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1921 ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc atcaggcata aagcagatta
     1981 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgag
     2041 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt
     2101 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcacc
     2161 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg
     2221 gacgacgatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat
     2281 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2341 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac
     2401 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2461 ttttgctaca ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg ctaccggacc
     2521 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac
     2581 ctgaatagtc tgtttgttca gtttcactac accagaagca cccagacgtt tacacatcgt
     2641 atcgttgacg cgggcagagt cagccacggt caggcgcaga cgggtaatac aggcatcaat
     2701 cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag
     2761 accttcttca gtattgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2821 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2881 gaagaagata acgcccatca ccagcagcat ccagacgttc tggctggcgg caggcaggtt
     2941 atacatcaac gcgtagtcga tagcacccgc agagaaagag aagccggcat ggatacccag
     3001 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3061 cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3121 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3181 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3241 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3301 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3361 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3421 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3481 aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc
     3541 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3601 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3661 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc
     3721 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3781 aattgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3841 cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     3901 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     3961 aaagccgtat ttttattttg ctgcaaattt tactgccgat gttctgtaat cagattgtta
     4021 gatcatctgc tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa cgcgtgtctt
     4081 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4141 acttatttta tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca aatccaggtt
     4201 acgcttaaag atgtctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4261 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4321 atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc ctgccgactg
     4381 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4441 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4501 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4561 aaaacatcaa ccttctcaac ggcaatgccc cggatatcga tgccgagtgc cgccagtatg
     4621 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4681 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4741 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4801 caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgatta
     4861 tggtgctggg tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt tgcgtgaacc
     4921 atatgtggac catcagctgt ctgcaactgc atccgaaagc gatcatggtg tgcgatgaac
     4981 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5041 atatcaaagg tctgtaattg ttatccctgc cctggttcct tgctcagggc aatatttttt
     5101 aaaatcgggg gtcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5161 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5221 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5281 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5341 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5401 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5461 tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5521 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5581 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5641 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5701 ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc gcggggatta cctttgccac
     5761 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5821 cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     5881 caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     5941 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6001 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6061 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6121 gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc tcgccgcagg
     6181 taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6241 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6301 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6361 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6421 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6481 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6541 ggcgtacggc ttggtcgtca tgacgccacc atcactctgt ttgatctcag cagcaaagtg
     6601 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6661 ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctgcgcga gctaatcgcc
     6721 atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg
     6781 catattcagg tagaaaactg ggggctggta gaagcactgg aagaacgttt taaagtgacc
     6841 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     6901 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     6961 tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag
     7021 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7081 gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt
     7141 gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7201 ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7261 atcaacttat ttaatccgca aaaaattgtt attgctggtg aaatcaccga agccgataaa
     7321 gtgctgctcc ctgctattga aagctgcatt aatacccaag cgctgaaggc gtttcgtact
     7381 aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg
     7441 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7501 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7561 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7621 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7681 agactgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg
     7741 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgccaggaa ggcaagaaag
     7801 cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7861 ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg ttcctacaac tgggacatga
     7921 tgcataaagc agcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     7981 cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct
     8041 ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc gcggcattaa
     8101 acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg cgataacctg cgtaccgata
     8161 ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcatcgc
     8221 tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa
     8281 tcgacgttat ctgaatacca cccacgtctg cggacgtggt tggctctgat gacaaacgcc
     8341 aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt
     8401 catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt
     8461 tatcagcaat ttggcccccg taaaatataa ccattcaata aaacccgcta aaaattatcg
     8521 attcatcaat aaatacatcc tcacaccaga ccctttttca ccaatcgaca atcaccatta
     8581 cgttttttat ttttgcactg ccaaatagct tgcgcacagt gctgctttgc ggcatttttt
     8641 taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa
     8701 tttttggcgt attcgatatc aaaacagacg cagttgagct gcgtaagaaa gcactcgagc
     8761 tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg
     8821 ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct
     8881 acaaccaaca aaaaaaccac gtactggcgg taaacggtga aatctacaac caccaggcat
     8941 tgcgcgctga atatggcgat cgttatcagt tccagacagg gtctgactgt gaagtgatcc
     9001 tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct
     9061 ttgctctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca
     9121 tcccactgta tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaat
     9181 cgctggtgcc agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc
     9241 aggacggcga aatccgttct tattatcatc gcgactggtt cgactttgat gcagtgaaag
     9301 ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc
     9361 tgatgtctga tgtgccttac ggtgtgctac tttctggtgg tctggattcc tcaattattt
     9421 ccgctatcac caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct
     9481 ggtggccgca gttacactcc tttgctgtag gtctgccggg ttcaccggat cttaaagcag
     9541 cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga aattcacttc actgtacagg
     9601 aaggtctgga tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta
     9661 ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa
     9721 tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg ttatctttac ttccataaag
     9781 cgcccaacgc taaagaactg catgaagaga cggtgcgtaa actgctggcc ttgcatatgt
     9841 atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt
     9901 tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa cccgcaggat aaaatgtgcg
     9961 gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa
    10021 gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca
    10081 ccctgaaaga agtggcggcg cagcaggttt ctgatcagca actggaaact gcccgcttcc
    10141 gcttcccgta caacacgccg acctcaaaag aagcgtatct gtaccgcgag atctttgaag
    10201 aactgttccc acttccgagt gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt
    10261 cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat ccgtctggtc
    10321 gtgcggttgg cgttcaccag tcggcgtata agtaagaaat gcggtactgc cccggagaaa
    10381 tccggggctg ttttagttat tgcgctgctt gttgattctg atatgcagta aggaaatgtt
    10441 ccgggtttgc cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta
    10501 atttcccacc actattgttg aattccgaat ttcatcctgt acgacaaaag aatggctgta
    10561 gggtggggaa aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa
    10621 cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc
    10681 tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa g
//
LOCUS       DAJTUN010000125        10588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTUN010000125 DAJTUN010000000
VERSION     DAJTUN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.125"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(82..435)
                     /locus_tag="OO470_003248"
     CDS             complement(82..435)
                     /locus_tag="OO470_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6252400.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(573..2219)
                     /gene="groL"
                     /locus_tag="OO470_003249"
     CDS             complement(573..2219)
                     /gene="groL"
                     /locus_tag="OO470_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6252401.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2263..2556)
                     /locus_tag="OO470_003250"
     CDS             complement(2263..2556)
                     /locus_tag="OO470_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6252402.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2832..4088
                     /gene="yjeH"
                     /locus_tag="OO470_003251"
     CDS             2832..4088
                     /gene="yjeH"
                     /locus_tag="OO470_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6252403.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4104..4580)
                     /gene="fxsA"
                     /locus_tag="OO470_003252"
     CDS             complement(4104..4580)
                     /gene="fxsA"
                     /locus_tag="OO470_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6252404.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            4917..6353
                     /gene="aspA"
                     /locus_tag="OO470_003253"
     CDS             4917..6353
                     /gene="aspA"
                     /locus_tag="OO470_003253"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6252405.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6471..7772
                     /gene="dcuA"
                     /locus_tag="OO470_003254"
     CDS             6471..7772
                     /gene="dcuA"
                     /locus_tag="OO470_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6252406.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7888..8226
                     /gene="cutA"
                     /locus_tag="OO470_003255"
     CDS             7888..8226
                     /gene="cutA"
                     /locus_tag="OO470_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6252407.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8202..9899
                     /gene="dsbD"
                     /locus_tag="OO470_003256"
     CDS             8202..9899
                     /gene="dsbD"
                     /locus_tag="OO470_003256"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6252408.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9936..10511
                     /locus_tag="OO470_003257"
     CDS             9936..10511
                     /locus_tag="OO470_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6252409.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2472 a         2780 c         2631 g         2705 t
ORIGIN      
        1 tcagttgcaa tgcctgctgt aggcctgata agcgaagcgc atcaggcaat aatacgtttg
       61 tcacccagcc tctggatcac atcagttcgg gcacttataa acctgaccga taatctggct
      121 atccgtcggg acaaaactgg acaacattcc ctgcgacggg ctgctgatgc cataaatcac
      181 gttaccgccc attgcagccg cctggttgcg cagatcgttt gctgcgccgc gcatagaacc
      241 gccctcttct ccgtgttgcc cggaaagcca gttgctttgc ttacctgtcg cagtaccaat
      301 cagctggcac tctgcgcctg gctgctcgtc cacaatgcgt acactctgac cggcagcact
      361 caattcgttg ctggagctac aacccgccat cagtagcgcg gcaccgacaa tccctgctaa
      421 gtattttacg tgcatgttat tccccataat caatgagctg gacgccactc gcgtcatccg
      481 tgtctgaatc ttatactaga aagatgacca aaagaaaaac ccccagacat ttctgcccgg
      541 gggtttgttt atttctgcga agtgcagggc aattacatca tgccgcccat gccacccatg
      601 ccgcccatac cgccagcagc gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc
      661 atgcattcgg tggtgatcat caggccagcc acagaagctg cgtactgcag agcagaacgg
      721 gttactttgg ttgggtccag gatacccatg tcgatcatgt tgccgtattc ttcggttgct
      781 gcgttgtaac cgtagttgcc gtcgccgcct ttaacggtgt tagcaacaac agacggttct
      841 tcgccgcagt tcaggacgat ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg
      901 atacccacgt tctggtcttc gttctgacca cgcaggtcag ccagtttaga cgctacgcgg
      961 atcagcgcaa caccaccacc agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc
     1021 agggcatctt caacgcgtgc ttttttctct ttcatttcaa cttcggtagc agcacctact
     1081 ttgataactg caacgccgcc tgccagtttc gctacgcgct cctgcagttt ttcacggtcg
     1141 tagtcagaag ttgcttcttc aatctgctga cggatctgag caacacggcc ctggattgca
     1201 gcttcttcac ccacgccatc gatgatagtg gtggtgtctt tgttgatcac aacacgttta
     1261 gcctgaccca ggtcttccag ggttgctttt tccagctcca taccgatctc ttcagagatt
     1321 acggtaccgc cagtcagggt tgcgatatcc tgcagcatag ctttacgacg atcgccgaag
     1381 cccggagctt taaccgcagc aactttcacg atgccacgca tggtgttaac aaccagagtt
     1441 gctagcgctt cgccttcaac atcttcagcg atgatcagca gcggtttgcc tgctttggca
     1501 acagcttcca gaaccggcag catttcgcgg atgttggaga ttttcttgtc agccagcagg
     1561 atgaacgggc tttccagttc tactgcgcca gtttccggct tgttgatgaa gtaaggagac
     1621 aggtagccac ggtcgaactg cataccttca accacgtcca gttcgtcctg cagaccggta
     1681 ccgtcttcaa cggtgataac gccttcttta ccgactttgt ccatcgcttc agcgatcagt
     1741 ttacctacgg tttcgtcgga gttagcggag atggtaccaa cctgagcaat cgctttagaa
     1801 tcagagcacg gtacggacag cgctttcagt tcttcaactg cagcggtaac cgctttgtcg
     1861 ataccacgtt tcaggtccat cgggttcatg cccgcagcaa cagctttcag accttcagtg
     1921 atgatagcct gagccagtac ggttgcagtg gtggtaccgt cgcctgcagc gtcgttcgct
     1981 ttggaggcaa cttctttcac catctgcgca cccatatttt cgaacttgtc ttccagttcg
     2041 atttcacgag caacggaaac accatctttg gtgatggtcg gtgcaccgaa agatttatcc
     2101 agaactacgt tacggccttt tggaccgagg gtaactttca ctgcatctgc cagtacgttt
     2161 acgccgcgca gcattttcac acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt
     2221 atctttattc cttaaattcg tatgttcagt gtcgtgcgcg gattacgctt caacaattgc
     2281 cagaatgtcg ctttcggaca tgatcaacac ttcttcattg tcgatcttct cggatttcac
     2341 accgtagcca tcgttgaaaa taacgatgtc gccaactttc acgtccagcg gcttcacttc
     2401 gccattttca aggatacggc cattgccgac agccagcact tcgccgcggg tggatttagc
     2461 cgctgcagag ccggtcagaa cgatgccgcc agcagattta gtttcaactt ctttacgctt
     2521 gacgatcacg cgatcatgca atggacgaat attcattgat aactctcctt tgagaaagtc
     2581 cgtatctgtt atgggtgacg ccggagctta cgtggtttcc cggctggtga ccagagaaat
     2641 ggggatgagg cttcgcccct tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa
     2701 tcgggtgaaa accctgattc acctcacatt tcatcgcaat ttcttcatcg ccgtcataag
     2761 cgaatctgat tgtgctacca tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg
     2821 gatatcaacc catgagtgga ctcaaacaag aactggggct ggcccagggc attggcctgc
     2881 tatcgacgtc attattaggc actggcgtgt ttgccgttcc tgcgttagct gcactggtag
     2941 caggcaataa cagcctgtgg gcgtggcccg ttttgattat cttagtgttc ccgattgcga
     3001 ttgtgtttgc gattctgggt cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg
     3061 gtatggcgtt tggttcgcgg cttgagcgag tcaccggctg gctgttttta tcggtcattc
     3121 ccgtgggttt gcctgccgca ctacaaattg ccgccggatt cggccaagca atgtttggct
     3181 ggcatagctg gcaactgttg ttggcagaac tcggtacgct ggcgttggtg tggtatatcg
     3241 gtactctcgg tgccagttcc agtgctaatc tacaaaccgt tattgccgga cttatcgtcg
     3301 cactgattgt cgctatctgg tgggcgggcg atatcaaacc tgcgagtatc cccttccccg
     3361 cgccaggaaa tatcgaactt accgggttat ttgctgcgtt atcagtgatg ttctggtgtt
     3421 ttgtcggtct ggaagcattt gcccatcttg cctcggaatt taaaaatcca gagcgtgatt
     3481 ttcctcgtgc tttgatgatt ggtctgctgc tggcaggatt agtctactgg ggctgtacgg
     3541 tagtcgtctt acacttcgac gcctatggtg aacaaatggc ggcggcagca tcgcttccaa
     3601 aaattgtagt acagttgttc ggtgtaggag cgttatggat tgcctgcgta attggctatc
     3661 tggcctgctt tgccagtctt aacatttata tacagagctt cgcccgcctg gtctggtcgc
     3721 aggcgcaaca taatcctgac cactacctgg cacgcctctc ttctcgccat atcccgaata
     3781 atgccctcaa tgcggtgctt ggctgctgcg tggtgagcac gttggtaatt catgccttag
     3841 agatcaatct ggacgctctt attatttatg ccaatggcat ctttattatg atttatctgt
     3901 tatgcatgct ggcaggctgt aaattattgc aaggtcgtta tcgactactg gcggtggttg
     3961 gcgggctgtt atgcgttctg ttactggcaa tggtcggctg gaaaagtctc tatgcgctga
     4021 tcatgctggt ggggttatgg ctgtttctgc caaaacgaaa aacgccggaa aatggcataa
     4081 ccacataatc cggcgtttcg acattaatcc tggcgatcgt ctttatgatc aaggcggtcg
     4141 cggtcatcat cctttcgctg atactcacca tcaaaagtat taccgccacc ggtcccggcg
     4201 ctaaaaccgc cgcctggcat gcgagaaaag cgcaaatgtg gcatcaactt cactgtcaga
     4261 tgcttttgca ccggcggcaa taaaagcaga agaccgagga agtcggtgaa aaagcccggt
     4321 aataaaagca gcaaaccggc aataatcagc gaaacacttt taatcatctc cgccgcaggg
     4381 ttttcacccg ccgccatttt ttgctgcatc agcacgaaat tcttaaagcc ctggttacgt
     4441 accagtgaca taccgataac tgacgtgaat ataaccagca cgagggtcag caataccccc
     4501 aatacatggg caacctgaat aaagattgaa atctcaatat agacataaag gaaaatggca
     4561 ataaaaggta accagcgcaa aggtttctcc tgtaatagca gccggttaaa cccggctacc
     4621 tgaatgggtt gcgaatcgcc ttcagcttat attgtggtca ttagcaaaat ttcaagatgt
     4681 ttgcgcaact atttttggta gtaatcccaa agcggtgatc tatttcacaa attaataatt
     4741 aaggggtaaa aaccgacact taaagtgatc cagattacgg tagaaatcct caagcagcat
     4801 atgatctcgg gtattcggtc gatgcagggg ataatcgtcg gtcgaaaaac attcgaaacc
     4861 acatatattc tgtgtgttta aagcaaatca ttggcagctt gaaaaagaag gttcacatgt
     4921 caaacaacat tcgtatcgaa gaagatctgt tgggtaccag ggaagttcca gctgatgcct
     4981 actatggtgt tcacactctg agagcgattg aaaacttcta tatcagcaac aacaaaatca
     5041 gtgatattcc tgaatttgtt cgcggtatgg taatggttaa aaaagccgca gccatggcaa
     5101 acaaagagct gcaaaccatt cctaaaagtg tagcgaatgc catcattgcc gcatgtgatg
     5161 aagtcctgaa caacggaaaa tgcatggatc agttcccggt agacgtctac cagggcggcg
     5221 caggtacttc cgtaaacatg aacaccaacg aagtgctggc caatatcggt ctggaactga
     5281 tgggtcacca gaaaggtgaa tatcagtacc tgaacccgaa cgaccatgtt aacaaatgtc
     5341 agtccactaa cgacgcctac ccgaccggtt tccgtatcgc agtttactct tccctgatta
     5401 agctggtaga tgcgattaac caactgcgtg aaggctttga acgtaaagct gtcgaattcc
     5461 aggacatcct gaaaatgggt cgtacccagc tgcaggacgc agtaccgatg accctcggtc
     5521 aggaattccg cgctttcagc atcctgctga aagaagaagt gaaaaacatc caacgtaccg
     5581 ctgaactgct gctggaagtt aaccttggcg caacagcaat cggtactggt ctgaacacgc
     5641 cgaaagagta ctctccgctg gcagtgaaaa aactggctga agtcactggc ttcccatgcg
     5701 taccggctga agacctgatc gaagcgacct ctgactgcgg cgcttatgtt atggttcacg
     5761 gcgcgctgaa acgcctggct gtgaagatgt ccaaaatctg taacgacctg cgcttgctct
     5821 cttctggtcc acgtgccggc ctgaacgaga tcaacctgcc ggaactgcag gcgggctctt
     5881 ccatcatgcc agctaaagta aacccggttg ttccggaagt ggttaaccag gtatgcttca
     5941 aagtcatcgg taacgacacc actgttacca tggcagcaga agcaggtcag ctgcagttga
     6001 acgttatgga accggtcatt ggccaggcca tgttcgaatc cgttcacatt ctgaccaacg
     6061 cttgctacaa cctgctggaa aaatgcatta acggcatcac tgctaacaaa gaagtgtgcg
     6121 aaggttacgt ttacaactct atcggtatcg ttacttacct gaacccgttc atcggtcacc
     6181 acaacggtga catcgtgggt aaaatctgtg ccgaaaccgg taagagtgta cgtgaagtcg
     6241 ttctggaacg cggtctgttg actgaagcgg aacttgacga tattttctcc gtacagaatc
     6301 tgatgcaccc ggcttacaaa gcaaaacgct atactgatga aagcgaacag taatcgtaca
     6361 gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct tttttcttgt gagcagtaac
     6421 ttaaaaataa caatctaata tcaacttgtt aaaaaacaag gaaggctaat atgctagttg
     6481 tagagctcat catagttttg ctggcgatct tcttgggcgc cagattgggg ggaataggta
     6541 ttggttttgc aggcggattg ggggtgctgg ttcttgccgc tattggcgtt aaacccggta
     6601 acatcccgtt cgatgtcatt tccattatca tggcggttat cgccgctatt tctgccatgc
     6661 aggttgctgg cggtctggac tatctggttc atcagacaga aaagctgctg cgccgtaacc
     6721 cgaaatacat cacgatcctc gcaccgatcg tgacctattt cctgactatc tttgctggta
     6781 ctggcaacat ctctctggcg acactgccag ttatcgctga agttgcgaag gaacaaggcg
     6841 ttaaaccttg ccgtccgctc tctactgcag tggtatccgc gcagattgcg atcaccgcat
     6901 cgccaatctc agcggcagtg gtttacatgt cttccgtgat ggaaggtcat ggcatcagct
     6961 acctccatct gctctccgtg gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct
     7021 tcctggtcac tatgctgttc aactccaaac tctctgacga tccgatttat cgcaagcgtc
     7081 tggaagaggg cttggttgaa ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa
     7141 aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt ggttatctat gcaatcatca
     7201 acagcccaag catgggtctg gttgaaaaac cgctgatgaa caccaccaac gcaatcctga
     7261 tcatcatgct cagcgttgca actctgacca ccgttatctg taaagtcgat accgacaaca
     7321 tcctcaactc cagcaccttc aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg
     7381 cgtggctggg cgatactttc gtttccaaca acatcgactg gatcaaagat accgctggtg
     7441 aagtgattca gggtcatccg tggctgctgg ccgtcatctt cttctttgct tctgctctgc
     7501 tgtactctca ggctgcaacc gcaaaagcac tgatgccgat ggctctggca ctgaacgttt
     7561 caccgctgac cgctgttgct tctttcgctg cggtgtctgg tctgttcatt ctgccgacct
     7621 acccgacgct ggttgctgcg gtacagatgg atgacacggg tactacccgt atcggtaaat
     7681 tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg tgttgccctg gccgtttgct
     7741 tcggcttcgt gctgggtagc ttcatgctgt aatgatcaat cgcggggcgt tcacgccccg
     7801 ctttctttcc cgccgactaa catccttccc ccgtccgttg tatagtgacc tctctcttgc
     7861 ggttccatct gttcttgcga ggtgtttatg cttgatgaaa aaagttcgaa taccgcgtct
     7921 gtcgtggtgc tatgtaccgc accggatgaa gcgacagccc aggatttagc cgccaaagtg
     7981 ctggcggaaa aactggcggc ctgcgcgacc ttgatccccg gcgctacctc tctctattac
     8041 tgggaaggta agctggagca agaatacgaa gtgcagatga ttttaaaaac taccgtatct
     8101 caccagcagg cactgctgga atgcctgaag tctcatcatc catatcaaac cccggaactt
     8161 ctggttttac ctgttacaca cggagacaca gattacctct catggctcaa cgcatcttta
     8221 cgctgattct gctactttgc agcacttccg tttttgccgg attattcgac gcgccgggac
     8281 gttcacaatt tgtccccgcg gatcaagcct ttgcttttga ttttcagcaa aaccaacatg
     8341 accttaatct gacctggcag atcaaagacg gttactacct ctaccgtaaa cagatccgca
     8401 ttacgccgga acacgcgaaa attgccgacg agcagctgcc gcaaggcgtc tggcatgaag
     8461 atgagtttta cggcaaaagc gagatttacc gcgatcggct gacgcttccc gtaaccatca
     8521 accaggcgag tgcgggagcg acgttaactg tcacctacca gggctgtgct gatgccggtt
     8581 tctgttatcc gccagaaacc aaaaccgttc cgttaagcga agtggtcgcc aacaacgcag
     8641 cgtcacagcc tgtgtctgtt tcgcagcaag agcagcacac cgcgcaattg cccttttccg
     8701 cgctctgggc gttgttgatc ggtattggta tcgcctttac gccatgcgtg ctgccaatgt
     8761 acccactgat ttctggcatc gtgctgggtg gtaaacagcg gctctccact gccagagcat
     8821 tgttgctgac ctttatttat gtgcagggga tggcactgac ttacacggcg ctgggtctgg
     8881 tagttgccgc cgcagggtta cagttccagg cggcgctaca gcacccatac gtgctcattg
     8941 gcctcgccat cgtctttacc ttgctggcga tgtcaatgtt tggcttgttt actctacaac
     9001 tcccctcttc gctgcaaaca cgtctcacgt tgatgagcaa tcgccaacag ggcggctcac
     9061 ctggcggtgt gtttgttatg ggggcgattg ccggactgat ctgttcacca tgcaccaccg
     9121 caccgcttag cgcgattctg ctgtatatcg cccaaagcgg gaacatgtgg ctgggcggcg
     9181 gcacgcttta tctctatgcg ttgggcatgg gcctgccgct gatgctaatt accgtctttg
     9241 gtaaccgctt gctgccgaaa agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt
     9301 ttgtgatcct cgcactgccg gtcttcctgc tggagcgagt gattggtgat gtatggggat
     9361 tacgcttgtg gtcggcgctg ggtgtcgcat tctttggctg ggcctttatc accagcctac
     9421 aggctaaacg cggctggatg cgcgtggtgc aaataatcct gctggcagcg gcattggtta
     9481 gcgtgcgccc acttcaggat tgggcatttg gtgcgacgca taccgcgcaa actcaaacgc
     9541 atctcaactt tacacaaatc aaaacggtag atgagttaaa tcaggcgctc gttgaagcca
     9601 aaggcaaacc ggtgatgtta gatctttatg ccgactggtg cgtcgcctgt aaagagtttg
     9661 agaaatacac cttcagcgac ccgcaggtgc aaaaagcgtt agcagacacg gtcttacttc
     9721 aggccaacgt cacggccaac gacgcacaag atgtggcgct gttaaagcat cttaatgtcc
     9781 ttggcctacc gacaattctc ttttttgacg gacaaggcca ggagcatcca caagcacgcg
     9841 tcacgggctt tatggatgct gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa
     9901 cgacacttgc agtgggaaag acggaggaaa atagcgtgca acgtgaagat gtactgggag
     9961 aagccctgaa attattagaa ttacaaggga ttgccaacac cacgctggag atggttgctg
    10021 aacgtgtgga ttatccactg gacgagctac gccgcttctg gccagacaaa gaggcaatcc
    10081 tctacgatgc gctgcgttat cttagccaac agatagatgt ctggcgtcgt cagctgatgc
    10141 tggacgaaac gcaaaccgcc gaacaaaagc tgctggcacg ttatcaggcg ttatcggagt
    10201 gcgttaaaaa caaccgctat ccgggctgtc tatttatcgc tgcctgtacg ttttatcccg
    10261 atcctggcca ccctattcat caactggccg atcagcaaaa aagcgctgcc tacgatttca
    10321 cccacgaact gttaaccacg ctggaagttg acgatccggc gatggtagca aagcagatgg
    10381 aactggtgct ggaaggctgt ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg
    10441 acactgcaca tcggctggcg gaagatatcc tgcgcttcgc ccgctgccgc cagggtggtg
    10501 cattgacctg acagaaacac agaaaagaag cgatttgccg caatcttaag cagttgaatc
    10561 gcttttactg aaattaggtt gacgaaat
//
LOCUS       DAJTUN010000126        10534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTUN010000126 DAJTUN010000000
VERSION     DAJTUN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.126"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(109..381)
                     /locus_tag="OO470_003258"
                     /pseudo
     CDS             complement(109..381)
                     /locus_tag="OO470_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(475..1037)
                     /locus_tag="OO470_003259"
                     /pseudo
     CDS             complement(475..1037)
                     /locus_tag="OO470_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1241..1447)
                     /locus_tag="OO470_003260"
     CDS             complement(1241..1447)
                     /locus_tag="OO470_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6252410.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1554..2117)
                     /locus_tag="OO470_003261"
     CDS             complement(1554..2117)
                     /locus_tag="OO470_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6252411.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3492..5327)
                     /locus_tag="OO470_003262"
     CDS             complement(3492..5327)
                     /locus_tag="OO470_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252412.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5428..5715
                     /locus_tag="OO470_003263"
     CDS             5428..5715
                     /locus_tag="OO470_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6252413.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5687..7216
                     /locus_tag="OO470_003264"
     CDS             5687..7216
                     /locus_tag="OO470_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6252414.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7386..8603)
                     /locus_tag="OO470_003265"
     CDS             complement(7386..8603)
                     /locus_tag="OO470_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6252415.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8970..9956)
                     /locus_tag="OO470_003266"
     CDS             complement(8970..9956)
                     /locus_tag="OO470_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252416.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9953..10481)
                     /locus_tag="OO470_003267"
                     /pseudo
     CDS             complement(9953..10481)
                     /locus_tag="OO470_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3280 a         2415 c         1985 g         2854 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcact tacaccagac
      121 atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca cagcttccct
      181 actccagagg taatataaag gggtgggctc gactgattta tctttacgtc gcttacactg
      241 caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca gagaaggaat
      301 tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa ttttaggatg
      361 tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg cgttacagga
      421 aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa taggtcaaaa
      481 accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg tcaaataatc
      541 cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa tatccaggat
      601 gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt ccaaccctaa
      661 ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct catagtttcc
      721 cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca tttaaaggac
      781 tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg tttgccttgt
      841 tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg gaaacgagat
      901 atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc aatcacagga
      961 gggtaatgga gatccccccg tattgctgtt attctggaag tgtttttaat attcgtattg
     1021 atagttctca gagatatatc acactctaga tattttaatt aagtatcagt tcgatatcgg
     1081 catattgtac actacctcag gtagatgatg agatcagata cagggatcaa ctgacggtga
     1141 gcgcagtccc cacattttat ccagggctta ctgcatattc ccaacagcca ttcgttagcc
     1201 aggtagacga ataccctgat ttgccgctgg ttttgctttc ctatcgaagg gcccatacat
     1261 catcacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg gataatacga
     1321 aaacgcactg cacagggctc tgcggtttac gtcgccattc aatcccatga gcttcaagta
     1381 atgagaccag ccgggtattt tctgcccgca gtactgctag ttcatcgctg tcagatttat
     1441 aaaccatatg cgttatcact tgtacctgtc tcccggcggc accacgactg aaattcgtgt
     1501 gaaggcaaat ccacgcgata acttacgata gttcggacat aagcccccat aattcagtta
     1561 tgtcgtggtc gaccccggtt acttttttcg ggaagcctgc tacggcccag aataataccg
     1621 tgttctttct gatcctgttt agccagatag ctaagggcgt aacgtaagtc gtctaccgca
     1681 gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc tttagcccgg
     1741 cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc cagttgcctg
     1801 gatatctgat aagagttctt gtgacgttgt ccatccaggt agccgataaa atgaatatga
     1861 agccctttgt ctggtgtata ttccattacc cagtaatatc ccgccaacat cgtctgggtt
     1921 tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg cccgaaactg
     1981 ggcgtgtctt tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga aaaacgttga
     2041 aacataccat ccatgtgttc attcacgtct ttcagaatca tgaaatccat tttgtaattg
     2101 ggattggcat tatacatttt atagtcctta ctttataaaa ctcacaggaa gagagaatta
     2161 cgtaaatact gagagtagag aaacgccgta ccacggcatt aataaacgaa gtaaaccagc
     2221 agcacagaca acctgataaa actgttttgt attaccgcat tactgtgatg aggtaatact
     2281 tcagaataca gtgagactct cttaaactga gaaccagtac gaattacaat ggaaaaacac
     2341 acctcataga attgagtttc aggaagcacc actaaattct ttacaggaag taatctgaca
     2401 ttcacaaaac tcagtatcgg ctagctacag gcaacatcgc tattaatatt gtcgaaccat
     2461 taagtaaagc cgagagagac cacgggtatt taatcattag cataaaagaa taatggtata
     2521 cagaacatac ccacacacca taagaagatt gttccataca gaaaagatca tatttaccta
     2581 tacctataaa ctgtaacgga gtcataacac gctctaatag tgttccgata cgtaaccagt
     2641 gttaagtacg tacaggacaa cattacacac ataattcacc atacagcttc ccccatatgc
     2701 ttcactgcgt agtgtatgga gatatatgca ttacacacat acagaatact tagcataaag
     2761 aggagtgaca ttaccacgta aagagataag ttatacaaca aacatacaaa caatacggca
     2821 cagacacaca ataccataac tccaactaat aatactaatt aaccttaatt atttatggta
     2881 tatgaaataa atgtgattct cccggaacta actaacctaa ccagacagat gatcactgta
     2941 taaaaacttc agttaatact catttgtttt gtcgactcag tatataatga gtgaccagta
     3001 atcaggataa tcacacttac ataacctgta aaaaagaaca agaagaataa gccactaaca
     3061 taatgcaaaa tggcatctga atcgatcgca gtttggcatt cgaatcgatc atgatttggc
     3121 ccaatccgtg atcgaataga gcggaatacg caacataatt caccagacaa gagcttacac
     3181 cgaattacag attatgtatt aatgtcaaaa ctggcatatt ttacagatac aatgaacatg
     3241 caacccatca atctaatagg ggcggtatgt acatctgccc atgaatggga tggcgctaag
     3301 aatacaaaac aattagacat agatcacaaa aacacaccat tctaattgaa aaacaaaaca
     3361 aacaaataaa tcatactatt atagaatttt ctcttgccag acatagataa tacccctgct
     3421 aattttgcta atggttagca aaattagcag ggtatataaa gattattgac ccaaaacaca
     3481 ctcagatgaa ttcacgacag ctcaggaggg ttattcctga tgtcatcttc acaaataatg
     3541 gttaacgcat catatatcat tctgcgtctg ttcctgtttt cggccttatc gccatacaac
     3601 atcatcacat ttttaatttc atcgaccggt gatttcacat agtgaactaa tacaccaagc
     3661 ttctcaacac attgcagtaa agtatatttc ttactgcctg tgaaaagccc tatcatagtt
     3721 tctttacgtt cgcgactgga taatactttg ttttccataa tgacttctgc cagcgtctta
     3781 ccatcataaa catactcttt atctatcaga atatttatct tatccagcac cttctctacg
     3841 tcatcaagca aacctgtaat atatgggggc gcaatatatg gtttcgtact gatcttttca
     3901 agcatacaat tatacatctt aataacacgc atcagggtaa aattgttcgc atcatactgt
     3961 atttcaccct ccttatatac aggatcaata agtggtattt tttcaataaa tgcatcctga
     4021 taggctttta catggtcaag ccaataaaac aacgagccat actttatatt ttttctattt
     4081 actttaacct ccagtgctgt ccgaagcaac gataatgccc ccctgacata tcctgaaggt
     4141 aattcaccaa acaatccttt tctttccatg tcatcataat gctgcagcac atcattaatt
     4201 ttatttttcc ttatcagaca aatgtacacc gcaagtgttt cgtagcagct ggtcacatga
     4261 acagcctcat tctgcaactg cccaaaatag cttataatct ttttcagata tgcgtcatca
     4321 caaccattct tatcattaat attaaataca agatctttca gacgggaaat atattcctga
     4381 gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact gttttgaaac
     4441 ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct ggtaaagtca
     4501 taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc tctcaccaac
     4561 actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat atcaagtaag
     4621 acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac tttctccagt
     4681 tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt gatagccatg
     4741 atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt tcctgtatat
     4801 ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa tcccagttca
     4861 gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt atcagcgtga
     4921 tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata aaaagcagag
     4981 tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac cccaaaagtg
     5041 cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg ataagaattc
     5101 atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc ctgactgaac
     5161 accccttcag catcaataat acgactgatc tcattataac gttgatccca gaatttttga
     5221 tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccttc cttaaacaac
     5281 cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg ctgtgctctc
     5341 cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa actgacacaa
     5401 cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct ttacacgcct
     5461 tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag atctactatt
     5521 tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa accaataagg
     5581 cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct gatgttacat
     5641 acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga tgacgacaaa
     5701 actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg cattacttga
     5761 tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta tgctgacggt
     5821 catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc aacgtaccag
     5881 ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct ctgcctgcgc
     5941 aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat atatcgacga
     6001 taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa tcctggagcg
     6061 gagatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac gagcagcact
     6121 tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca tcgtgaatga
     6181 cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc tgttgctgtc
     6241 tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac tgtacaacac
     6301 attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt tccggctgaa
     6361 tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata agttttttac
     6421 tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat tttgcgaacc
     6481 cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg tgacgcaaca
     6541 atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact ctctgagagc
     6601 atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat caaatgcagc
     6661 atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac agaatcctca
     6721 tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca ggatagcagc
     6781 acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa gtactaatag
     6841 cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg aatcccttcg
     6901 ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta tcacaacagc
     6961 aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc tcaggaaaaa
     7021 atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa ggattgatga
     7081 taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa aaaaactaaa
     7141 tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac tgaagtatca
     7201 ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg tattattcgt
     7261 tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg aactggcatt
     7321 gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta tgccagtcca
     7381 tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata atccgcccat
     7441 gcctgcatca tcggtacacg ctgctccagt agatcagtac gatgatatgc cgcctcaacc
     7501 ttatttttca gcgtatgagc gagcgccctt tccgccagat cccgcgaata cccctgttca
     7561 ctacaccagt ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg ccccggaatg
     7621 tcactgacgg ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga aaggatctgc
     7681 tgctttctgg gtgaagggaa caccagttca tcatgcaggc cacgtatatt ttccagaatg
     7741 taaatagcct gccgggataa aggaacacga tgctgtagcc tagctttcat tctttctgca
     7801 ggtatagtcc atacccgctt atgaaaatca atttcagccc agcgcattcc cctggcttcg
     7861 cccgagcgag ttgctgtaag tatcaccatt aataacagtg cgcgggtaac attataaggt
     7921 tcatcactat acacactggt cgccacaaaa agcggtaact gcctccaggg cattgcgggt
     7981 tggtgttcat cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac atcaacagga
     8041 tttgccacac aaaaaccgtg cgcccatccc cactgcataa caacatgaat gcgctgttta
     8101 acccggcttg ccgtttctga caaggttaac cagactggac gcagtgtttc tgccacgtcc
     8161 gcagccgtaa tcgaatccag cgtttttgct cccagttgag gaaacgcgta attctcaagc
     8221 gtcgataacc actgccttac atgctttgga ttctcccatc caggagacag ttctgcatgt
     8281 acacgcctgg ctgcatcggc aaatgttggg ataacgactt tctcagattc agccttttta
     8341 atctccagag gatcatcacc tgcagcaagt tgctctcgca ttatccgggc agtacgtgca
     8401 gcttcagcaa tactgacctc tgggtaagtt cccaatccag cattacgtct tttttgtgtc
     8461 accggactta cataacgaaa aacccatttc ccccgcccct ttactgaaga aggatgaagg
     8521 gtcagtccgg taattccccc atggggcaat ggtttgtcat caggtttgat atgtcttgct
     8581 ttcgtatccg tcaatactgc catacgctaa ttcccgtcac tctggtatgc catccagtat
     8641 gccattagtc cctcgcttca ctaagaatac attagataac atcggacaac aaaaagtgta
     8701 atacattgtt tataaagaaa ttatataaca cccctggatt aagtcagatt tatttcaggc
     8761 ggtccccctc accgccatat ttaaagaaga gcgcgtacga aagtacgggc ttttttttcg
     8821 tatattgtac acaccgggtg cccccgccca cgtctgttag ggcgagggga aattgtgcac
     8881 atcaactgtc tggttactca gtcaacaatc aaaatgcgta agatagtaca gaacatatat
     8941 gcgttatctg gtcacttcct gtaacttcct cacttattaa aatgccccca acggaaatac
     9001 atgtcacatg ccatcataaa atcgttatgc cagaggaatc gacaatggaa aaaatagtaa
     9061 attaagcacg gtacatagca gagtaaaaaa taggcttcaa accaaatccg ccattccaca
     9121 agcgttgcca ataccgacac cagcgtaatc cacatcattt caataacccg caatatgaat
     9181 aaccgaagcc aaagcggctt ggcaaaaaag gtcgacaacg tattttcact ggagaaaaac
     9241 agatcgattt cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga gaagcgcaga
     9301 aaaaacagat taatcgccaa cactacgcca caaagaatga gcaatgcaat tatcgattta
     9361 aacgttaacc actgcccctc tgtcacaaaa gaatcactgt tacgcaacgg gctgatagta
     9421 agcaggttaa caaccgttga gatgcacacc gaaagcagca catagcgagt cagactttta
     9481 tattttactt ttttcgctac tgaaatagcg acctgcttga cataactggt gcggatcatc
     9541 cgaaacagca gaaagatgac caacggccca agtaacgaaa tcaggaacag cgcatataac
     9601 gagggtagaa gctggtatag cccccaactc attgcactca acatgattaa cgacagaaat
     9661 cctgccacca gaggttgtgt aagaaataat tctattttgt ggttctgctt tttcttaaac
     9721 cacgaaaaat cgatattctt cgtgacgcat ttataagccc agtacgcctt gagatcaaaa
     9781 ataaaattat acagcaccag ggccagaatc ccactaacta caccaatatc ctctgatgga
     9841 aaagtaaacc ctgcattttt ccaataaaag cctatcagaa taagtccaat agcataaatg
     9901 tatatcagat ttcgtttatc ctgaaagcga ataaaagtta aataatcagg aatcatttga
     9961 atacctttgc atcactaaat gctttacgac aatgcataac tcgttgctga tccacgccag
    10021 caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg aaagctaccg
    10081 gggttggcat attcagaata tgtatcgaga gattcagtcc atttcaccgt atgagttaag
    10141 tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc tgttacgttc
    10201 atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc gcgcattgcc
    10261 tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag tttaaccggt
    10321 tcgtatccag cggccatcag cagcgtggta aattcattca acgcagcctg cgttccccca
    10381 ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc tgattccgta
    10441 atcatcgtct gcagttgttc gcggttgatg cctttaaccg cctgatctta cccagcaata
    10501 gtggacacgc ggctaagtga gtaaactctc agtc
//
LOCUS       DAJTUN010000127        10384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTUN010000127 DAJTUN010000000
VERSION     DAJTUN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.127"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            427..624
                     /locus_tag="OO470_003268"
     CDS             427..624
                     /locus_tag="OO470_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252417.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(574..1767)
                     /locus_tag="OO470_003269"
     CDS             complement(574..1767)
                     /locus_tag="OO470_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6252418.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1903..3627
                     /gene="iucA"
                     /locus_tag="OO470_003270"
     CDS             1903..3627
                     /gene="iucA"
                     /locus_tag="OO470_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6252419.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3628..4575
                     /gene="iucB"
                     /locus_tag="OO470_003271"
     CDS             3628..4575
                     /gene="iucB"
                     /locus_tag="OO470_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6252420.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4575..6317
                     /gene="iucC"
                     /locus_tag="OO470_003272"
     CDS             4575..6317
                     /gene="iucC"
                     /locus_tag="OO470_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6252421.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6314..7651
                     /gene="iucD"
                     /locus_tag="OO470_003273"
     CDS             6314..7651
                     /gene="iucD"
                     /locus_tag="OO470_003273"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6252422.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7657..9852
                     /gene="iutA"
                     /locus_tag="OO470_003274"
     CDS             7657..9852
                     /gene="iutA"
                     /locus_tag="OO470_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6252423.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2517 a         2651 c         2754 g         2462 t
ORIGIN      
        1 ggagttggtc ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc
       61 atcagtgagt caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa
      121 ttaaaaacca gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat
      181 gtcagtgaaa aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct
      241 cgccaatgcc ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac
      301 ctgtttgaac gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa
      361 tttaataaca gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa
      421 agaatcatgg agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg
      481 cagcaagcca gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct
      541 ctgatttact ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat
      601 aaatacagac acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat
      661 tgcgacacct gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt
      721 gtgactggta gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag
      781 ttgataagcg ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa
      841 caaccatagc tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact
      901 caggagcagg gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag
      961 cgcagctact gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc
     1021 cgtagcgaac tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc
     1081 agccagtaat attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt
     1141 gcactccggt gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag
     1201 cactggggct gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc
     1261 cgcgcacagc ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc
     1321 ggccatgcga ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat
     1381 tgccgacacg gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc
     1441 tgttgttatg gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat
     1501 gccatatcct cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt
     1561 tccccaccac ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca
     1621 tacagacaga ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt
     1681 ctgtccgata ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg
     1741 gggcgtatct tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt
     1801 tatttgtaaa attaatttat ctgacaataa catttattgt tgataatgag aatcattatt
     1861 gacataattg ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa
     1921 aaaccagcca cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca
     1981 gactggaaac tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa
     2041 tcgctccatt tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct
     2101 gcacgtctgg tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt
     2161 attattgata agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac
     2221 cagcgcgtgc tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg
     2281 gccgctctgc gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga
     2341 cacgctttcc accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc
     2401 tacctgcctg atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa
     2461 atcgccggtg aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag
     2521 aatgcgcctc agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg
     2581 tggcaggggg aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg
     2641 attaaagact taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc
     2701 tactgtgcca ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc
     2761 atccgtactc tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa
     2821 accgacggct ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat
     2881 ggctgggccg ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg
     2941 cgtgaaaatc tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact
     3001 caggccgcac ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat
     3061 cgcctcggta tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa
     3121 gtgctaaagc cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag
     3181 caaaatattc ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt
     3241 cagggcagcg cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag
     3301 gcggaaaata ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt
     3361 aactccactt ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat
     3421 ctgatggctc gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc
     3481 tgtctcaact acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat
     3541 ctgaacgatc ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct
     3601 aacccgctgc aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat
     3661 ggactgcgct gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc
     3721 gcggtgttgc actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag
     3781 atatttatcg ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag
     3841 gagccacagg cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc
     3901 ttctggcagt taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt
     3961 tttgatgcag agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt
     4021 taccgccgtt acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt
     4081 tctgatgcag aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag
     4141 caaagtggct cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat
     4201 gcattcccgc tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg
     4261 gcggcggaag accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat
     4321 ctgctggttg gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg
     4381 ctgacacatt acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc
     4441 accgataacc agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag
     4501 ttcgacttcc cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg
     4561 gaggtcggtc tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa
     4621 aaatgttgtc tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc
     4681 actactgcat taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg
     4741 gctggctgtg gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga
     4801 cgctgctgat gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata
     4861 tgcaggattt gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac
     4921 tgagtgccag tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc
     4981 atcctaaatt cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat
     5041 atgcgccaga gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata
     5101 tgatctggcg ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc
     5161 cgcaggagtt tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc
     5221 tgccgctgcc ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg
     5281 attttgccga aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc
     5341 agtcgctgcg taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc
     5401 tgaccatcta caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac
     5461 cactggcttc acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg
     5521 gcgcagtgat ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc
     5581 ttgcccgggc tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt
     5641 gccgctggct gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg
     5701 aaaacaatca gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct
     5761 ggcttacgca attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg
     5821 gtgtcgcgct tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac
     5881 agcgtgttct gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg
     5941 aaatggactc tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact
     6001 taatccatga tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga
     6061 tggttcgtct tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg
     6121 attacatgaa caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc
     6181 cacaaatcat tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg
     6241 gcagtcgcat gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc
     6301 aggaatatga atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa
     6361 tctcagcatt gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga
     6421 acatcctcat ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt
     6481 ctttctgaaa gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta
     6541 tctggtgaag cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg
     6601 tgaagagttt tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag
     6661 tcataccgtt gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag
     6721 tcggggagaa tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc
     6781 accctgtgtg aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg
     6841 gccggacctg agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct
     6901 gttccttaat gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg
     6961 gcgtaataat tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga
     7021 atatatttca ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca
     7081 gaaaatgaca tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta
     7141 ccaccgtttt gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt
     7201 aacaactctg gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca
     7261 gggcagagag agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt
     7321 gccacaaata cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa
     7381 agtgcgtgat gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt
     7441 caacgccagc atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg
     7501 atctgcacgt attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc
     7561 cgcactgatt cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt
     7621 aactcactat acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg
     7681 gctcttggtc cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat
     7741 aatgagatca tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc
     7801 acctgggtta tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa
     7861 gacgcactgg ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac
     7921 ggtatgaaca tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct
     7981 tcacgttccg acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa
     8041 gtgatctccg gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc
     8101 gtgaccaaaa aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc
     8161 tttaacagca gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac
     8221 catatctccg gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac
     8281 ggcgatgcca ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac
     8341 atcatgggaa ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag
     8401 tactataaaa gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc
     8461 atcagcggga gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc
     8521 actgagcggc atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg
     8581 gtcggtcagg tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat
     8641 gcgaataaac aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg
     8701 aaactgactc tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct
     8761 gagcatgagc gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc
     8821 ggagggctga acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc
     8881 aatctggcgg ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt
     8941 ggcgtacgct atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa
     9001 cagaagattg ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat
     9061 tacgataact ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca
     9121 tggttcaatt tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc
     9181 atctatggtg cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac
     9241 agtaagctgg aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac
     9301 aacctgcgga ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat
     9361 aaagatctga ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg
     9421 gtggactacc tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa
     9481 accgagtcga aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg
     9541 aaagcgacag cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc
     9601 acttctttcg acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat
     9661 tttatcagta gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc
     9721 gaccgtgact ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac
     9781 ggccctgctt cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca
     9841 gtgctgttct gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg
     9901 gtggccatgt tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg
     9961 aatgggctgt tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt
    10021 atcgcatatc ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc
    10081 agcaccgtct ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc
    10141 cggaactttg ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc
    10201 tacagggtaa aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc
    10261 tcctgcgcct gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat
    10321 cgtcagtgcc cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc
    10381 gaat
//
LOCUS       DAJTUN010000128         9946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTUN010000128 DAJTUN010000000
VERSION     DAJTUN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.128"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(334..579)
                     /locus_tag="OO470_003275"
     CDS             complement(334..579)
                     /locus_tag="OO470_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252424.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1224..1299)
                     /locus_tag="OO470_003276"
     tRNA            complement(1224..1299)
                     /locus_tag="OO470_003276"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1264..1266),aa:Phe,seq:gaa)
     gene            complement(1405..2112)
                     /locus_tag="OO470_003277"
     CDS             complement(1405..2112)
                     /locus_tag="OO470_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6252425.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2510..4645
                     /gene="speC"
                     /locus_tag="OO470_003278"
     CDS             2510..4645
                     /gene="speC"
                     /locus_tag="OO470_003278"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6252426.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4694..5950)
                     /gene="nupG"
                     /locus_tag="OO470_003279"
     CDS             complement(4694..5950)
                     /gene="nupG"
                     /locus_tag="OO470_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6252427.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6152..7231)
                     /gene="mltC"
                     /locus_tag="OO470_003280"
     CDS             complement(6152..7231)
                     /gene="mltC"
                     /locus_tag="OO470_003280"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6252428.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7296..7571)
                     /locus_tag="OO470_003281"
     CDS             complement(7296..7571)
                     /locus_tag="OO470_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6252429.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7599..8651)
                     /gene="mutY"
                     /locus_tag="OO470_003282"
     CDS             complement(7599..8651)
                     /gene="mutY"
                     /locus_tag="OO470_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6252430.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8812..9531
                     /gene="trmB"
                     /locus_tag="OO470_003283"
     CDS             8812..9531
                     /gene="trmB"
                     /locus_tag="OO470_003283"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6252431.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9531..9857
                     /locus_tag="OO470_003284"
     CDS             9531..9857
                     /locus_tag="OO470_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6252432.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
BASE COUNT         2428 a         2416 c         2605 g         2497 t
ORIGIN      
        1 gaggagtccc cagaaaagaa gacaggcata gagttttgtg atctactgat tgcgctaaca
       61 aattcaatga gatcacctct atgcctgccc gttaagtatg tcaggctttt ttccgtaatg
      121 ctctaacttc cacgcattgg tttctgacat caaataaggc tatgactttc tctccctttt
      181 aatctcattc tgatctaaaa ccatatctta aaaaaatgca tgggtatttt aattattttg
      241 atgttgatct aacgcactca aggaccattt tattaccgat tatccattct atttaactgt
      301 tgcgatataa atgtttgttt taggtgcttt aattcagtac aagtcatcaa accgtaccag
      361 accctgactg tcagtgacac cgacaggtgg gggcggcggt gtggggtcaa actgagtgtg
      421 tctgcctctg gcccacagag ccagctctgc cgccattgcc cgtggatgat actccagctg
      481 cacgttgaga ccggaaactg ggttgttgtt ctcatccacg aactgcagcg ccagccagtg
      541 catccgctgc ttaccgcagg cgacttcttt gtacttcatt ttgtgactca ggcaggtgac
      601 tgtcgtgttg cctgttgcgt ccattttgcg gttaaaccct tttaattcaa tttgttaaat
      661 tgctttccac cttttgcggg gcgcgactta accaaaatat tcactgttat cataatatgc
      721 aatgggctgg aggttgcagg tcatttaggg attttctgta agccccgttt tgcagaacgt
      781 tctatctccg attgagatcc tgtctctcgg tattaccggt agtctggcca taatccccta
      841 tggatcatat tgatatagta ggcataacac ccaaaagttg actaaagcta aatcaatcag
      901 agtgaaaaat gttcggtttg atgtaggggg ggactgagga ggggccaacg caggtggtat
      961 gcgttttact tcagaccgga caaagaccgg atgtatccgt gcagtaatga atccgttagc
     1021 caggtgctca tgtggaaata gtgtctattg gggatttgaa gtttgacaga aaaggccaca
     1081 gtgaccagaa gggggctatt tagaagaaat gttcaggctg gatggaagag gtaaagcagt
     1141 gacatcagca ttcaaagtga ccggatatct accagaaccc cagaaagcaa aaaaccagcc
     1201 cgtaggctgg ttcttctaaa tagtggtgcc cggactcgga atcgaaccaa ggacacgggg
     1261 attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca ttaaacctga
     1321 ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca atctatcatc
     1381 actttgccgc tattgcacgc attgtcaggc aaaccaggcg gtccaggcgg cggaaagcgg
     1441 cattgccaga aggagtgctg gaagcatgtt gaccaccggg aacattttaa tgccacagat
     1501 gcgtaatccg gttgccagca gcaataaacc gcctacggcg ctgaagtctg ccatcatcga
     1561 cggtgtgatc agcggtaata tcagcgcggc agcccacgcc agcgttaact ggatgatcag
     1621 taatgggata ctaattaccg acaccgcaat acccagcgag caggcgaaga tcatcgccgt
     1681 aaagaaatca agaaatgact tggcgattaa aatactcgga tcgccggtca tcccttcgtt
     1741 catcgccccg aagatcccgg tgccgctggc gcaaaacagg acaataatcg cgacataatt
     1801 ctgaataaaa gattcatgcg ctggcttctt acgtgagtga cgaaacagat tttgtgcttt
     1861 ggcgactgct gtattgacac ctttctccag caaacaaatt tcgccaatta gagcgccaag
     1921 tagcgtagct aaaaccatcg ctggaaggtt ggcgcatttc acaaccagta aaataccaat
     1981 ccccagcgat gccagaccaa aaatagatgt catggagacg cggatacgtt ccggtaagcg
     2041 ttggctgagc agtgcgccga gaacgccacc cagtaagact gcactagcgt tgataaaagg
     2101 gccgatgacc acaagagttc ctgttagctg tattgtttga ttttcttatt atgaactttt
     2161 tgccttgttg atggcttgtc tggtcgcaaa gtcctggctt gcacgcctta gcgaaaggtg
     2221 ccatgattgc gcgaattttc tcctctctgt acggagtttg cccgatgcac gccatctcct
     2281 tacattctct cgcttatcgc cgtttcgcgc gaaacgtttc cctttctatg ttactgctca
     2341 tgcggtgaag ttaacgcacg ctcactgcag gacaacagta aaatcagagc gtttctgctt
     2401 ttactgatgt ctggcggtcg gagctggtga ccagtttgac ccatatctca tggggcaggg
     2461 ttttccacct tgtccggtat tcttacttcc ccgaaacggg tttgcgctta tgaaatcaat
     2521 gaatattgcc gccagtagtg aagtggtatc ccgactttct tctcatcgcc gcgtggtggc
     2581 gttgggagat actgatttta cggacgtcgc ggcagtcgtc attaccgctg cggatagtcg
     2641 cagtggcatt cttgcgttgc ttaagcgcac cggttttcat ctaccggtgt ttttgtattc
     2701 cgaacatgct gttgaattac ctgcgggcgt tacggcggta atcaacggca acgagcagca
     2761 gtggctggag ctggaatccg cagcctgtca gtatgaagag aatttgctgc caccgtttta
     2821 tgacacgctg acgcagtacg ttgagatggg caacagcacc tttgcttgcc ctggacatca
     2881 acatggtgcg ttctttaaaa agcatcctgc cggacgccat ttttacgatt tctttggtga
     2941 gaacgtcttt cgcgccgata tgtgtaacgc tgacgtaaaa ttgggcgatc tgcttattca
     3001 tgaaggatcg gcgaaagatg cgcagaaatt cgcagccaaa gtctttcatg ccgataaaac
     3061 ctattttgtg ctgaacggca catcggcagc gaataaagtg gtgacgaatg cgctgttaac
     3121 gcgtggcgat ctggtgctct tcgaccgtaa caaccataag tcgaatcatc acggcgcgct
     3181 gattcaggcg gggacgacgc cggtctatct ggaagcttca cgtaacccgt ttggtttcat
     3241 tggcggtatt gatgcgcact gttttaatga agagtatctg cgccagcaaa ttcgcgacgt
     3301 tgcgccagaa aaagccgacc tgccgcgccc gtttcgcctg acgattattc agctgggaac
     3361 ctatgacggc actgtctata acgcccgtca ggtgatcgat accattgggc atctgtgtga
     3421 ttacattctg tttgattccg cgtgggtcgg ttacgaacag tttatcccga tgatggcgga
     3481 tagctcgccg ctgctgttag aacttaacga aaacgatccg gggatttttg tgacccagtt
     3541 ggtgcacaaa cagcaggctg gattctcaca gacgtcgcag atccataaaa aagataacca
     3601 tatccgcgga caggcgcgtt tttgcccgca taagcggttg aataacgcct ttatgctcca
     3661 tgcttctacc agccctttct atccgctgtt tgctgcactg gatgttaacg ccaaaattca
     3721 tgaaggggag agtgggcgtc ggctgtgggc tgagtgcgtt gagttgggta ttgagtcgcg
     3781 caaggctatt cttgcgcgct gtaagctgtt ccgcccgttt atcccgcccg ttgttgatgg
     3841 caaattgtgg caggattatc cgacatcagt gttagccagc gaccgccgtt ttttcagttt
     3901 tgagccgggg gcgaagtggc acggctttga aggatatgcc gcggatcagt attttgttga
     3961 tccgtgcaag ctgttactca ctacaccagg tatcaatgcc gaaaccggcg aatatagcga
     4021 ctttggcgtt ccggcgacga ttctggcgca ctatctgcgt gagaacggca ttgtgccgga
     4081 gaagtgcgat ctcaactcca ttctgttctt attaactccg gcggaaagcc acgagaagct
     4141 ggcacaactg gtggcgatgc tggcgcaatt tgaacagcat attgaggatg actcgtcgct
     4201 ggctgaggtg ttgccgagcg tttataacaa atatccggtg cgctatcgcg actacaccct
     4261 gcgccagttg tgtcaggaga tgcacgatct gtatgtcagt ttcgacgtca aagacctaca
     4321 aaaagcgatg ttccgccagc agagtttccc gtcagtggtg atgaaccccc aggatgcgca
     4381 tagcgcttat attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat
     4441 tgcggcagaa ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt
     4501 ctggggtggg gcggttcaac gttatttcct tgcgctggaa gaaggcgtga atttgttgcc
     4561 gggattttcg ccggagttgc aaggtgtcta tagcgaaacc gatgcggatg gcatgaaacg
     4621 attgtacggt tatgtgttga agtaagaata aaaaaacggg tcaccttctg gcgacccgtt
     4681 tttctttgcg taattagtgg ctaaccgtct gtgtgcctgt cgggacacga acgtgtttat
     4741 atttgaacat cgccatgaac gcgaaggcca gaaccacgga ataaccagcg aaaatcaacc
     4801 atacggtctg ccagtcggta atgccgtttt gggtgtacat ctcaacaact ttaccgctca
     4861 cgatgccgcc gaggatacag ccgaagccgt tagtcatcat caggaacatc ccttgtgcac
     4921 tggcgcgaat tgccgggcta acttcttttt cgacaaacac cgaaccagag atgttgaaga
     4981 agtcgaatgc gcaaccgtaa acgatcatcg acagtaccag cagtacagta ccgaacggag
     5041 tcgggtcgcc gtaagcaaac agcgcaaaac gcaggatcca cgccacaata ctgatcatca
     5101 ttacgttctt aataccgtag cggcttaaga agaacgggat ggtcagaatg aacagggttt
     5161 cagagatctg cgaaatcgac atgatgattg acgcatgctg cacgatgaag ctgctggcaa
     5221 acatcggatc tttgtcgaag ctgtgcagga aggtattacc gaacatgttg gtaatctgca
     5281 gttccgcgcc cagcagcatt gagaagatga agaagattgc catacgcttg tttttaaaca
     5341 gcgcgaatgc atcgaggccc agcagggttg tccagctctg attcgcttgc tgtttagcaa
     5401 ccggaatatg cggcagagtc agggtaaaca gaaccagaat ggcggaaagt gctgcgccaa
     5461 tatacagctg catgtggctt aattcgaagc cagacaggct caccacccac attgccatga
     5521 taaagccgat ggtgccccag atacggattg gcgggaagtc agtaacgata tccatcccgg
     5581 cattttgcag gcgatagtaa gagatggtgt tgattaaccc aagcgttggc atataagcaa
     5641 acgagttaat caatatcaca aggaacatcg cttccggcgt cgtgacctgt gccgccatga
     5701 acagcgtgat agcgccaatg gtgtggcaaa tggcatatac ccatttcgca cttaaccatt
     5761 tgtcggccac aatccccagc agcgcaggca taaataccgc ggcgataccc agtgagctat
     5821 aaactgcgcc aatagaagca ccgtcaaact tcagggtaac aaacatatag gagccgaggg
     5881 tcgtcagcca acttccccac agacagaact gcagaaaaga gaggattttc agctgcagct
     5941 taagattcat gttaatttcc tcacatcgtg atgcggatga gtgtttgaaa aagcacattt
     6001 gaccgtgcga gatgatggga ttctatcaac ttcgcggact ggttttttgt tacctgtggc
     6061 aaataattgc aaacattccc aatttgcaaa acgaaattgt gatgtggata acatttttgc
     6121 ccctgagcat cgtcaggggc ggttaatgga attatcggcg gcggtaggat ttttgcgcgg
     6181 tattcacttt ataaagataa cggcgagatt ccgcagaggg atggcgggtc gtcagcgtct
     6241 gataaacatc gcccggcgtc atggtgttaa taatattggc agcctgaatc ttatcattcg
     6301 aaaagactcg cagcacgctg cctgcgccgc cgttataggc ggtgatgacg gcataacgcc
     6361 gcgatgttgg gttatcaatt ccgccgagat aaacattgtt cagcatcgcc agatacgcgg
     6421 tgccggtatc aatattgctg gcaggatcaa acaagaaact gcggctcggc gtgccggatt
     6481 tcccctgcga gcggaacaca tctttcccgg cagtattttg taccacctgc attaatccca
     6541 gcgcatcgga acggctgacc gcatacgggt taaaggaaga ttcagtctgc ataattgcca
     6601 gaatcagcga ctcatcaacg ccatatttcc gtgacgcctg gcggaccatg ccgagatatt
     6661 tgtgcgcacg tttatcaagg tggttcggca ccatgttaat ggtgacgctg tagatgatac
     6721 gtagcccgtt gctgcggctt ttcagacggt ttttcagcag ataatccgcg aagttgcttg
     6781 cgcgaccttc ccagcgaatc ggctgcccgg tgttgtccac cacctgaccg taaaggaaag
     6841 gttctttcga aattgtaata tcatcaacgt cggaatagag atcgaccgaa ctcggatcgt
     6901 cacccatcag caacgttttg ataattgccc ggcgcaaatg cgcggcaggt tctgtcccgg
     6961 cgatggtttc gatagtaatc gtaccgtcat cgaagttgat gtggctgcgg gtctgatatt
     7021 gatcggtgta tttcacgtag tccttaggac cggcgatcac cacctctttg aagccccaga
     7081 tgttctcaat attgtgggca aattgcccca tcagaatatc aaaaccgttg gtatctttga
     7141 cccaggcttc gttataggta tcgccttttt tggtcgtcga acaggagatg agcaacggcg
     7201 caatcaaagc cagcgcgaga tattttttca tcattccggg tgcgtgttgt gtttgttttt
     7261 atgcaagctg ccggaggcgt gctccggcac tgtttttatt ttttatcttc cggcgtatag
     7321 ccctcgatat gcacctcttt accctcgaac aggaagttga ccatctcctg ctccagcagc
     7381 ttgcggtgct cggcattcat catgttgagt ttcttttcat taatcagcat ggtttgtttg
     7441 tgctgccact gcgcccaggc ttctttggag atctcgttat agatgcgttt tcccagctcg
     7501 ccggggtaca gctgaaaatc ctgaccttct gcttcacgtt gcaggaaagt acaaaaaatc
     7561 gttctgctca taaatcatcc tctttatcga ctcacgcgct aaaccggcgc gccagtgcgt
     7621 aactgctgta ataaacgctc cacgggagcc gccaggccaa ctgacggcgg ttgcgctaag
     7681 ttataccaga gcgcattgcc ttcatccatg cagccggtga atgacgacac gggaagccac
     7741 ataggcacaa tatctaagtg gaaatggctg aaggtatggc gaaacgcggt cagttgcgtc
     7801 aggttatcgg cagcaatctg ccgttgtgcc agccaatgcc gcaaactttc ttcgtcggca
     7861 aactgcggga aacagtataa accgccccac aatccgctcg gcggacgctg cgccagcaat
     7921 acttcatctt cgtgctgtaa caggagaaag tagccggtgc gctccggcag cgtctgtttc
     7981 ggttttttgc ccggataaag cgaccagcta ttgttggtgg cggcaataca tccgttttgt
     8041 agcggacaga gcgaacattt cggcttcgaa cgcgtgcaaa tcatcgcgcc caaatccatc
     8101 atcgcctgat taaaccgttc cacgccgacc gcgggcgtca cctgctcgct caaactccat
     8161 agtttattct cgacctcttt tttcccaggc cagccgctta cagcatagca gcgcgccagc
     8221 acgcgtttga cgttaccgtc gagaatcgga aagtgcttac ccagagaaag cgagagaatc
     8281 gcgcctgcgg tggaacgccc gacgcccggt aacgccgcga cttcttcaaa ggtttccggg
     8341 aatttaccac cgtgtaaggt cgccacttgt tgtgccgctt tatgcaggtt gcgcgcgcgg
     8401 gcgtaatagc caagcccggt ccacaagtgg agaacttcgt cgagcggcgc attggcgaga
     8461 tcggtcaccg tcgggaagtg cgccataaag cgttcaaaat aggggataac ggtcgcaacc
     8521 tgagtttgtt gcaacatcac ttctgagagc catactttgt agggcgtctt gtcaatttgc
     8581 cagggcagcg ttttccgccc gtatttatcg taccagtcca gaacctgggc tgaaaattgc
     8641 gacgcttgca tggtcaccga cttcactgtt gttgggggca agattgcagc acagcgacgg
     8701 cagggtgtaa accggaactt tccgcagcca cggccttatc atgcttgcat ccggcaacta
     8761 actttggata atgcccgttt tcagaacact ttcacaagcg actaaatctt tatgaaaaac
     8821 gacgtcattt caccggaatt tgatgaaaac ggccgcccac tgcgccgtat ccgtagtttt
     8881 gtgcgccgcc aggggcgact gaccaaaggc caggaacatg cgctggaaaa ctactggccg
     8941 gtgatgggcg ttgagttcag tgaagatatg ctggatttcc ccgcgctttt tggccgtgaa
     9001 gcgccggtga cgcttgagat tggttttggc atgggggcgt cgctggtggc aatggctaaa
     9061 gatcgccccg agcaggactt cctcggcatt gaagtgcatt caccgggcgt tggtgcgtgc
     9121 ctggcttctg cgcatgaaga aggtttaagc aacctgcgcg tgatgtgtca cgatgcggtt
     9181 gaagtgctgc ataaaatgat tcctgacaat tcattgcgca tggtgcagct ctttttccct
     9241 gacccgtggc acaaagcgcg ccataataaa cgccgtatcg ttcaggtgcc gtttgccgaa
     9301 ctggtaaaaa gcaaactgca gctggggggc gtattccata tggcgaccga ctgggaacct
     9361 tatgcggaac atatgcttga agtgatgtct tctattgacg gttataaaaa cctgtcagag
     9421 agcaatgatt acgtaccgcg tccggcatca cgtccggtga cgaaatttga acaacgtggt
     9481 catcgtcttg gtcacggagt atgggactta atgttcgaga gggtgaaata atggcaaaga
     9541 accgtagccg tcgtctgcgt aaaaaaatgc acatcgacga attccaggaa ttaggatttt
     9601 cggtggcatg gcgattcccg gaaggtacat cggaagaaca gattgataaa atcgttgatg
     9661 actttattaa cgaggttatc gaaccgaaca aactggcctt tgacggcagc ggttatctgg
     9721 cctgggaagg tctgatctgc atgcaggaaa tcggcaaatg caccgaagaa catcaggcga
     9781 ttgtgcgtaa gtggctggaa gagtgcaaac tggatgaggt acgcaccagc gaacttttcg
     9841 acgtttggtg ggactaagaa agcatacggg cgatgacaaa tgcaaaactg cctgatgcgc
     9901 tacgcttatc aggcctacag agatgcacga tcgagtaggc gggata
//
LOCUS       DAJTUN010000129         9830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTUN010000129 DAJTUN010000000
VERSION     DAJTUN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.129"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            330..461
                     /locus_tag="OO470_003285"
     CDS             330..461
                     /locus_tag="OO470_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6252433.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            721..1716
                     /gene="pgl"
                     /locus_tag="OO470_003286"
     CDS             721..1716
                     /gene="pgl"
                     /locus_tag="OO470_003286"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6252434.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1757..2710)
                     /locus_tag="OO470_003287"
                     /pseudo
     CDS             complement(1757..2710)
                     /locus_tag="OO470_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2894..3946
                     /locus_tag="OO470_003288"
     CDS             2894..3946
                     /locus_tag="OO470_003288"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6252435.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4022..5455
                     /locus_tag="OO470_003289"
     CDS             4022..5455
                     /locus_tag="OO470_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6252436.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5638..7818
                     /locus_tag="OO470_003290"
     CDS             5638..7818
                     /locus_tag="OO470_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6252437.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7959..9242)
                     /locus_tag="OO470_003291"
     CDS             complement(7959..9242)
                     /locus_tag="OO470_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6252438.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9377..>9830)
                     /locus_tag="OO470_003292"
     CDS             complement(9377..>9830)
                     /locus_tag="OO470_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6252439.1"
                     /translation="VVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDA
                     LGISMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFH
                     ELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2435 a         2288 c         2468 g         2639 t
ORIGIN      
        1 atgttttaca tattcacaat gcagaggatt tataccagag gacattagtc ttgagtaaga
       61 tactacaaca tctccaacta ttttagccag tctgactact tgacttggta atatttgttg
      121 ctaataatca tgtataaaat aagcttatat acatagtaac ttcagcaaag gatttagtaa
      181 tgatcaaaat catatctcaa tgtactgttt ttttctttaa aaaaacagta cattctattt
      241 tataatgact gtgtattccc atggaaggag atcacccaat tctccccccc gacttttgac
      301 gcagtgtctt gttaacaggg ggattgacca tgcttattgg ctatgtacgc gtatcaacaa
      361 atgaccagaa taccgatcta caacgtaatg cgtttatttg tgccggaagt gagttgattg
      421 ttgcgcgaat gggaagttgg ccagaagttg ttatggcata gtgtttacgg tcgttgatac
      481 aggtaaacaa aagcggggag agtcatactc cctgaatagt cgcgaaaata tctgtggcat
      541 tgtcaaattc gtgtcggtga ctaatatttt agtaatatgc cattcgaaat ggagtgctaa
      601 cttattgatt taattgatgt gcgatattgt attttatcgt gttttgttgt ctgtttttta
      661 tccagtaagt gttgttatat gtatgaaaga taagggattt atcatttttg aggtgtgttc
      721 atgaagcaaa cagtttatat cgccagccct gagagccagc aaattcacgt ctggaacctg
      781 aatcatgaag gcgcactgac gctgacacag gttgtcgatg tgccggggca ggtgcagccg
      841 atggtggtca gcccggacaa acgttatctc tatgttggtg ttcgccctga gtttcgcgtc
      901 ctggcgtatc gtattgcccc ggacgatggc gcactgacct ttgccgcaga gtctgtgctg
      961 ccgggtagtc cgacgcatat ttccaccgat caccaggggc agtttgtctt tgtaggttct
     1021 tacaatgcgg gtaacgtgag cgtaacgcgt ctggaagatg gcctgccagt gggcgtcgtc
     1081 gatgtagtcg aggggctgga cggttgccat tccgccaata tctcaccgga caaccgtacg
     1141 ctgtgggttc cggcattaaa gcaggatcgc atttgcctgt ttacggtcag cgatgatggt
     1201 catctggtgg cgcaggaccc tgcggaagtg actaccgttg aaggggccgg cccgcgtcat
     1261 atggtattcc atccaaacga acaatatgcg tattgcgtca atgagttaaa cagctcagtg
     1321 gatgtctggg aactgaaaga tccgcacggc aatatcgaat gtgtccagac gctggatatg
     1381 atgccggaaa acttctccga cacccgttgg gcggctgata ttcatatcac cccggatggt
     1441 cgccatttat acgcctgcga ccgtaccgcc agtctgatta ccgttttcag cgtttcggaa
     1501 gatggcagcg tgttgagtaa agaaggcttc cagccaacgg aaacccagcc gcgcggcttc
     1561 aatgttgatt acagcggcaa gtatctgatt gccgctgggc aaaaatctca ccacatctcg
     1621 gtatacgaaa ttgttggcga gcaggggcta ctgcatgaaa aaggccgcta tgcggtcggg
     1681 cagggaccaa tgtgggtggt ggttaacgca cactaaccgc tgatttaacc ggcgcagtct
     1741 ctcctgcgcc ggtgtattaa cttatctcct gtaacgcatg tctctggcgt tcgacgatat
     1801 tggtccacaa attgtcttta tcgtcagtcc acaaattaat cagcaaggaa aaaaagcgtt
     1861 cagctgccgg agaaagcacg gcatctttgc ggcgaataat ccccaatgtc cgacgtataa
     1921 cgggttccac cagtgggata ccaataatcg aagaataggg cgcatggggc attgccagac
     1981 caggaagcgc cgaaatcccc agtcctgcct ccactagtcc taatgacgtc gaaagatgac
     2041 gcacttcgta aaaccagtcc agcttccagg gcttgtcggc tagctgttgt tctatcaaca
     2101 atcggttgcc gctggaggaa cgtacgccaa tcattttgta acccactaac tcctgccatt
     2161 ctacaagcta ctttttggcc aatggatggt cacgccgaca ggccagcacg aatggctcgt
     2221 tgaccagtgg ggtaaaatca atggatgaat ttgtgacgtt gttcatgttt atgccaaagt
     2281 ctgattcgtt acacagcacc gattccatgc aattattcgt gccttgttcc agaatccgca
     2341 ctttaatatt gggatacagc tcattaaatt taccgattgc cagcggtaaa aaataaaata
     2401 ctgcggtcgg aatacacgct aatgtcacca tatcacgatg ataagcattc atatcacgaa
     2461 tattaaaaag cgtttcatcg aatttcttta ttaattcccg cgcctccggg agcaaccttt
     2521 ttcctgcttt tgttaacgta accttgcgag ttgtacgctc aaatagttga atatgtaaat
     2581 cttcctccat cttttttatt cttcgcgtta atgcaggttg cgtaatattg agtaatttag
     2641 cggcattgtt aaatgaactg gactctgcca gtatgacaaa tgccttcata cttgataatt
     2701 catgcttcat tattactccg gaaaatggaa gcgacgattt tggggggctg gtcgttaaaa
     2761 attttaactg catttagcca acttaaatta atgaaaaaat gttattaatc gttgagctaa
     2821 agtcattaga gatgctttgt ccttaatgta accatatcgc aataagttat gtttttaaat
     2881 tgagggcatt attatgaaaa aaataccctg cgtgatgatg cgaggtggaa cctcgagggc
     2941 cgcgttcctg ttagcggaac atttacccga agatcaaacg cagcgcgata aaatattgat
     3001 ggcaattatg ggttccggta acgatctgga aattgacggt attggcggcg gtaatccact
     3061 gaccagtaaa gtcgccatta ttagccgttc cagcgatccg cgtgctgatg tcgattatct
     3121 gtttgctcaa gttatcgtcc atgagcaacg tgtcgatacc acgcctaact gcggcaatat
     3181 gctgtctggc gtaggggcat tcgccattga aaatggtttg attgcagcga cttcgccagt
     3241 tacccgtgta cgtatccgca acgtcaatac gggtacgttc atcgaagctg atgtgcaaac
     3301 gccaaatggt gttgtcgagt acgagggtag cgccagaatt gacggcgtac cgggtactgc
     3361 cgcaccggtt gcgctcactt tcctgaatgc cgctggaacg aaaaccggaa aagttttccc
     3421 gactgataat cagattgatt attttgacga tgtcccggtg acctgtatcg atatggcgat
     3481 gccagtcgtc attattccgg ctgaatatct gggaaaaaca ggttatgaat taccggcgga
     3541 actggatgcc gacaaagcat tattagcccg cattgaatct atccggctac aagcgggtaa
     3601 agcaatgggc ttaggtgatg tcagtaatat ggttatccct aaacctgtgc ttatttctcc
     3661 agcgcagaaa ggcggggcaa ttaatgtgcg ttattttatg ccgcattctt gccatcgcgc
     3721 gctggcgata accggtgcta ttgctatttc cagtagttgt gcattggaag gcaccgtcac
     3781 ccgacaaatc gtcccttctg taggatatgg caatatcaat attgaacacc ccagtggtgc
     3841 gctcgacgtt catttaagta atgaaggtca ggatgccacg acgttacgcg catctgttat
     3901 tcggacaacc agaaaaatat tttccggtga agtttatctt ccctgaaaaa attcgttgtc
     3961 aggataagga caatcaataa aggacttctg tatgagtcat acagaaagaa caggatttta
     4021 aatgaataag aaatcgttat ggaagctaat tctgatatta gcgatcccat gtattattgg
     4081 ttttatgcca gctccggcag gattaagcga actggcgtgg gtgctttttg gtatttacct
     4141 ggcggccatt gtgggtctgg ttatcaagcc tttcccggaa cctgtcgtac tgttaattgc
     4201 cgttgctgcc tcaatggtgg tggtcggtaa cttatccgac ggtgcgttta agaccaccgc
     4261 cgtattaagc ggttactctt caggtactac ctggctggtg ttctcggcgt ttaccttaag
     4321 cgccgcattt gtgaccaccg gtttaggtaa acttattgcc tatctgctga ttggtaaaat
     4381 cggtaacacc atgctgggtc tgggttacgt tacggtattc ctcgatctgg tactggctcc
     4441 ggcaacaccg tctaacaccg cgcgtgcggg cggcattgtg ttaccgatca tcaacagcgt
     4501 ggcgatggct ttggggtccg aaccggaaaa aagtccgcgt cgtgtcggac attacctgat
     4561 gatgtccatt tacatggtca ccaaaaccac cagctatatg ttctttaccg caatggcggg
     4621 gaacattctg gcgctgaaaa tgatcaacga cattctgcac ctgcaaatta gctggggtgg
     4681 atgggcgctg gccgccggat tgccgggcat cattatgctg ctggtcaccc cgctggtaat
     4741 ttacaccatg tatccaccag aaattaaaaa ggtggataac aaaaccatcg ctaaagcggg
     4801 ccttgccgaa ctgggaccga tgaaaatccg cgaaaaaatg ctgctcggtg tctttgtgct
     4861 ggcgctactg ggctggattt tcagtaagtc tctgggggtt gatgaatcca ctgtggcaat
     4921 cgttgttatg gcgaccatgc tgctgctggg tatcgttacc tgggaagacg tggttaaaaa
     4981 taaaggcggc tggaatacct taatctggta cggcggtatt atcggcttaa gctccttatt
     5041 atcgaaagtt aaatttttcg aatggttagc tgaagtcttt aaaaataacc tggcatttga
     5101 tggtcacggt aacgttgctt tcttcgttat tattttcctc agcatcatcg tgcgttattt
     5161 cttcgcttcc ggtagtgcct atatcgttgc tatgttacct gtatttgcca tgctggcgaa
     5221 cgtctccggc gcaccgttaa tgttaaccgc gctggcactg ttgttctcca actcctatgg
     5281 cggcatggtt actcactatg gcggcgcggc aggtccggtc atctttggcg tgggttacaa
     5341 cgatattaaa tcctggtggt tggtcggtgc ggtactgacg atattaacct tcttggtgca
     5401 tatcaccctc ggcgtgtggt ggtggaatat gctgatcggc tggaacatgc tgtaaatata
     5461 ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag tcacttactt
     5521 atgtaagctc ctggggattc actcacttgt cgccttcctg caactcgaat tatttagagt
     5581 atatccattt attatctttc tgcgcacttc acggtgcgca gatatctgga gcatttgatg
     5641 atcaagttat ctgaaaaagg cgtgtttctc gccagtaata acgaaataat tgccgaagaa
     5701 catttcaccg gcgaaattaa aaaagaagaa gcaaaaaaag gcactattgc ctggtctatt
     5761 ctctcttctc ataatacgtc cggaaatatg gataaactta aaattaagtt tgattcatta
     5821 gcctctcacg atattacctt tgttggtatt gtacagaccg ctaaagcgtc cggaatggaa
     5881 cgtttcccgc tgccgtatgt gctgaccaac tgccataact cactctgcgc cgtcggcggc
     5941 accattaacg gtgatgacca tgtttttggt ttatcggcag ctcagcgtta tggcggtatt
     6001 tttgtgcctc cgcatattgc ggtcatccat caatatatgc gtgagatgat ggctagcggg
     6061 ggcaaaatga tcctcgggtc agacagccac acccgttacg gtgcattagg gacaatggca
     6121 gtcggtgagg gtggcggtga gttggtaaaa cagctgctta atgacacctg ggatatcgac
     6181 tatccgggcg tggttgcggt gcatctgacc ggaaaacctg cgccgtatgt ggggccacag
     6241 gatgtggcgc tggctatcat tggcgcggtg ttcaaaaacg gttacgtcaa aaacaaagtc
     6301 atggagttcg ttggaccggg cgttagcgcg ctctctaccg atttccgtaa cagcgttgac
     6361 gtgatgacca cggaaaccac ctgtttaagc tccgtctggc aaactgatga agaagtgcat
     6421 aactggctgg cgctgcacgg tcgcggccag gattactgcc agcttaaccc tcaaccgatg
     6481 gcgtactacg atggctgcat cagcgttgat ttaagcgcca tcaaaccaat gattgcgctg
     6541 ccgttccacc cgagcaacgt gtatgaaatc gacacactga accagaacct gaccgacatt
     6601 ctgcgtgaga ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa actctcgctg
     6661 ctggataaag tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc gggctgttct
     6721 ggcggtaact acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca atcctgtggc
     6781 aatgacacct tctcgctggc ggtttacccg tcatcacagc cggtgtttat ggatctcgcc
     6841 aaaaaaggtg tggtagcaga tttgattggc gcaggcgcaa tcatcagaac cgcattctgt
     6901 ggcccatgct ttggcgcggg cgatacgcca atcaacaacg gtttgagtat tcgccacacc
     6961 acgcgtaact tcccgaaccg cgaaggctct aagccagcca atgggcagat gtcagcggtg
     7021 gcgttgatgg acgctcgttc tatcgctgcg actgcggcaa acggtggcta tttaacctct
     7081 gccagcgaac ttgattgctg ggacaacgtg ccggagtacg ccttcgatgt aacgccgtat
     7141 aaaaaccgtg tttatcaggg ctttgtgaaa ggggcaactc agcaaccgct gatttacgga
     7201 ccgaacatta aagactggcc ggaattgggt gcgctgactg acaatatcgt cctgaaagtg
     7261 tgctcgaaga tcctcgacga agtcaccacc accgacgaac tgattccttc cggtgaaacc
     7321 tcttcttatc gttcaaatcc gattggtctg gcggggaatg tcagcgagct gacagaggtg
     7381 tttgcgcgca ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt
     7441 ggcagcatgg tctatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc
     7501 tgccagcgtg tgattggcgg tctggcgaat attgccgagg agtacgcgac taaacgctac
     7561 cgttctaacg tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt
     7621 gaagtggggg attacattta catccctggc attaaagcgg cgctggataa tccgggtacg
     7681 acgtttaaag gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctttacatg
     7741 gaaagcctga cggctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat
     7801 aaaaaccgtc agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc
     7861 ggatgcgatg ctggcgcatc ttatccgacc tacaaatcgc atcgaaccgt aggccggata
     7921 aggcgtttac gccgcatccg gcaaatagtt aatagctctt acttcttcgc ctctgcaacc
     7981 actttgctgc ccacgccgcg gttattgtat tcccacatgc ggttgtagtt agtgtcattc
     8041 agattgcgct gtatttcgtc gttatcatct acgctgccgg tattacccgc aaacggacga
     8101 ttagagatca ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt gatggcgcta
     8161 tcacggatca ccacctgacc gttggtatta gcatcaacat ccagcgagcg gcccagttgc
     8221 gccacgccat caccggaagc attgaaacgg ctgtttacgg cgaggaaacc gtaatagatg
     8281 ttagacagcg tagccggtgc aaacacatat gcttcttgct gggtacgtga gttcaccacg
     8341 cggaattcgg tgttatcgaa caccactgcg ccgcgaccag aaacgatatc cacatcccct
     8401 tcaatatagc tgttggtcac cagcgtacgc ggctgacggt tggtttccag acggttctgc
     8461 acaccgctgt tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac gttgttaatc
     8521 tgtacttggt caccgtcagt acgcagtgct accgccggat ggttacctgc atctacgcta
     8581 tcgcccagcg tgttttcgat ggtcagattt tgcagttgca gtccattgtt ttgtgaccag
     8641 aagaccgcag aacacagaac accgatactg tcgctgcgtt tgctctggca gctatcgtac
     8701 atataccacg ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag
     8761 tcggcagggc tcatgccccc atcaagggaa agcccaatct tcacatcaat cggtttttca
     8821 cctgtaccgt acagagtgat tccacctgga gcagcaggga catacaccgt tccctgatac
     8881 tccccaggca tcacggcaat atactggcgc ttgttggtgc gcttgataat tgccgcatct
     8941 accgccgcct gaatcgtggt atgcgttaca ccttgagtac ccgccgggcc gacaacaaag
     9001 tcaggttgcg caggcagggt tatcggggac ggattccacg ctgcagcacc tggtgtcagg
     9061 gatgcaaaat agtgttgcgc atcgaaattc agcgcttctt ttgccgacag aatcgggcga
     9121 gaagaggtac caggcgcagt ttgatcagaa ggacgttgat cgggtggtgt tgagctacag
     9181 gcggtcagcg tcacgccaaa agccaatgcc agcgccagac gggaaactga aaatgtgttc
     9241 acaggttgct ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc ttttttatac
     9301 taagttggca ttataaaaaa acattgctta tcaatttgtt gcaacgaaca ggtcattatc
     9361 agtcaaaata aaatcattat ttgatttcaa ttttgtccca ctccctgcct ctgtcatcac
     9421 gatactgtga tgccatggtg tccgacttgt gcccgagaag atgttgagca aacttatcgc
     9481 ttatctgctt ctcatagagt cttgcagaca aactgcgcaa ctcgtgaaag gtaggtggat
     9541 ccccttcgaa ggaaagacct gatgcttttc gtgcgcgcat aaaatacctt gatactgtgc
     9601 cggatgaaag cggttcacga cgagtagatg caattatggt ttctccgcca agaatctctt
     9661 tgcatttatc aagtgtttcc ttcattgata tcccgagagc atcaacatgc aatgttgtag
     9721 ggatggcaat ttttacgcct gttttgcttt gctcgacata aagatatcca tctacgatat
     9781 cagaccactt catttcgcat aaatcaccaa ctcgttgccc ggtaacaaca
//
LOCUS       DAJTUN010000130         9807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTUN010000130 DAJTUN010000000
VERSION     DAJTUN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.130"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1190)
                     /gene="baeS"
                     /locus_tag="OO470_003293"
     CDS             complement(<1..1190)
                     /gene="baeS"
                     /locus_tag="OO470_003293"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6252440.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGV"
     gene            complement(1187..2602)
                     /locus_tag="OO470_003294"
     CDS             complement(1187..2602)
                     /locus_tag="OO470_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6252441.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(2603..5668)
                     /gene="mdtC"
                     /locus_tag="OO470_003295"
     CDS             complement(2603..5668)
                     /gene="mdtC"
                     /locus_tag="OO470_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6252442.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(5669..8791)
                     /gene="mdtB"
                     /locus_tag="OO470_003296"
                     /pseudo
     CDS             complement(5669..8791)
                     /gene="mdtB"
                     /locus_tag="OO470_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(8791..>9807)
                     /gene="mdtA"
                     /locus_tag="OO470_003297"
     CDS             complement(8791..>9807)
                     /gene="mdtA"
                     /locus_tag="OO470_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6252443.1"
                     /translation="GLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDPS
                     QFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGTI
                     KADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDLV
                     FTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKAR
                     FNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLVT
                     PGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREYAKK
                     GARS"
BASE COUNT         2251 a         2790 c         2708 g         2058 t
ORIGIN      
        1 acacctggcg cactgtcggc aaaggtcagg cgcaccgttt tgtcgtgctg ctcggcagag
       61 attttcaggc tgccgccgct gtcagtgtag cgcaggctgt tttccagtaa gttattgaat
      121 aactgcatta aacggtcgcg atcgccaaat acggtgatac tgtctggcag ggaaaattgc
      181 agtttcaggc cacggctggc gaatcgttcg cgaaatgcgc cgcccgccac ttccagcagt
      241 gggatcaaat ctaccggtgc tttttgatag gcgagagcgc cttcatcaga catcgacaac
      301 tgatgaagat cgtcaaccag tttggtcagt gtaccgacct ccgcctgtaa agacgccacc
      361 gtctccggcg tgaatttgcg cacgccatcc tgaatggctt ccagttcacc gcgcagcacc
      421 gctaatggcg tacgcagttc gtgagaaata tcggccataa aatcgcgccg catttgctgg
      481 tttttctcca gcgtgctggc gagctggttg aagtcttgcg ccagtttgcc cagttcatct
      541 tcactggtgg gcgttacgcg ggtagtgaaa tcgcccgccg ccagtttgtg cgtgccatcg
      601 acaagtcgtt ttaccggtgc cagcaaaccg cgcgccagca gaaaggtggc gagcgccgcg
      661 agtaacgttg ccagggcgac gatcagccag ctggtttgcc gctgttgttt atcgaaattg
      721 atatcagtat tgcgggtcag ccgctcaaca ggggaggcaa tcaccgcgcc cacttccgca
      781 ccgttgacca gaatgggtcg ccgtgtaccg tcaggtggaa tcggcgctcg cggaccaacc
      841 agtactttgt tgttttgatc gaccacccag aactgggtac gccagccgtg cggcggcata
      901 cccgggccgg gtttatcttc cgaattatcg tgttcaaatg aacgcaggat ctgaaagaca
      961 aagcgatcgt tgttgcgcag gaagcgccag ttaccatgct gcgcatactg ctcgccaagc
     1021 gcatcactta acagctgtaa tcgctgttca ttgccgtgtt tgatgtaatc aataaagcca
     1081 cgctcaaaac tgatacgcac cgcccagtgc atactgatca gcaagacaat gcaggtggcg
     1141 aaaatcgcca gaaacagttt gccggtaata ccgggtcgcc agaacttcat tgcgcgctcc
     1201 tttttcgccg cgaaatagct acattttgat gcgtatcgtt cggcactctg gcaaagatga
     1261 acgccggaag ggcgatgata aacgccatgc taagccaggt gtacataaag acggtttgtg
     1321 tggtgccgct gtcgacgctg atatgctgcg aaccaaaaag ccccagcaac agcccggcga
     1381 tggtgacgcc gatactcatc gacaattgca taatcatcga cagcaggctg tttccgctgc
     1441 tcgccagatt gtccgggaga tctttcagcg tcagggtgtt catggaggag aaacgggtcg
     1501 agttgaccat cccttgtaaa aacaggacga acggcaaaac gtagtaccag cccaacagag
     1561 cggtagtcat aaacaacagg gtgaccagcg acagacccag cgtggtcgct accagtaccc
     1621 gacgataacc aaagcgattc accacctgta ccacaattcg cttcattccc atgctgccaa
     1681 gcaccatcgg gatcatcatc agtccggcat gaaacggcga gaaaccgagg ccaatttgca
     1741 ggaaaaccgg tgtcataaag ggcaacatgc cactgccaat acgtccggca aagctccccg
     1801 ccaggcccag cgaaaaggta cgagtgcgga acagtttcag actgaacagg gcgcggttgt
     1861 tatttctggc gtgcagcaga taaagcacca gtgccaccac gccaactgcg accaggccag
     1921 caatcgccag cggcgataaa cctgtacctt tactgccgtc cagcgccagg gttaataccg
     1981 ccatgccaac cgccagcaat aaaaagccgg aaagatcaaa gcgccgcgtc tgcatggtgt
     2041 agttcggcat taacatcaat gtggcgatcg caccgataat ccccaccgga atgttgatca
     2101 aaaagatcca gtgccacgac gcgtattcta ccagcagacc gccgagcgcc ggaccgagca
     2161 gcggaccgac ctgaccgggt aacgtgacaa aggtcatcgc cgccatatat tgctcgcgcg
     2221 gtacgatttt catcaccgtc aacctgccga ccggcaccat catcgcaccg ccaacgccct
     2281 gtaacgcgcg tgccagcaac agttcgttca gcgtgccgga aagcgcgcaa aacagtgaac
     2341 cgagagtaaa cagcacgatg gcggtaaaga aaatattgcg cacgccgact ttgtccgcca
     2401 gccagccgct ggcgggcagc atcaccgcca cggtcagcac ataagagaca atgaccatgt
     2461 gcatatgcaa cggactttcc ccgaggcttt gcgccattga gggaagggcg gtgtttacga
     2521 tggtggtgtc cagcgactgc ataaagaagc cgaaagccac aatccataat tgccagcggg
     2581 tgctgtcggg aagatctgtc atttactcgg ttaccgtttg tttaggttta cgcgaaaaac
     2641 gcagccgcag acggtcgaaa aagagataca ccaccggcgt ggtatacagc gtcaggagct
     2701 ggctcatcac cagaccgccg acaatggtga tctccagggg ttgccgcagc tccgagccgt
     2761 caccgcccga caataccagc ggcagcgcgc caaacagcgc cgccagggtg gtcatcataa
     2821 tcgggcgaaa acgcagcaga caggcctgga aaatagcttc ctgcggcgtc aggttaccgt
     2881 gccgttgggc ttcaagcgca aaatcgacca tcataatggc gtttttcttc acgatgccga
     2941 ttaatagcat gatccctatc agggcgatta ggctgaacgg ggcattgaac agctccagcg
     3001 ccaacagcgc tccaacgccc gccgagggca gggtggagag aatcgtcagc ggatgtacgt
     3061 aactctcgta aaggataccc agcacgatat acaccgtggc gatggcggcg ataatcagga
     3121 tcacctgcga gttcatcgtc tcctggaaaa cctgcgccgt gccggcaaaa ctgccgcgca
     3181 ccgtcgaagg cacaccaagc tgggtcattg cgcgatcgat cgccgcactg gcgtccgaga
     3241 gcgattttcc ggtcggcagg ttgaacgaga tggtcgaggc cgccgataat ccctgatgat
     3301 tcaccgatag tggggcattc gccggttgcc atttagcgaa atacgacagc gggatcgctt
     3361 tgccttcgtt attgataacg aacatttttt ccagcgcact gatgtcctgg gtatagcgcg
     3421 gatccacttc catcaccact ttatactggt tcatcggctg gtaaatggtc gagatttgcc
     3481 gctgaccgaa ggcgttattt aacagactgt tggcggcctg tacgtcgatt cccagccgtg
     3541 ccatggtgtc gcggtcgtaa accagattca tctccgcgcc gttatcctgc tgatcggagt
     3601 tcacgtccgc cagttccggc aacgtcgcca gttttttgcg gattttcggc tcccattcgc
     3661 gcagtgccgc caggtcgtcg gataacaacg tgtactggta gctggcgttc gactgacgcc
     3721 caccaacgcg aatatcttgt accgccatca ggaacagatt cgcccccggt tctttcgcca
     3781 gcttcacgcg cagacggtcg ataatttgct gcgccgtttc gctgcgttcg tcgcgtggct
     3841 tgagggtgat aaacatcatc ccactgttaa ctcgtgaacc gccggtaaag ccggtgacat
     3901 tatccactgc cggatcgtca cggataattt tcatgaaatc ctgcaatttg ccgcgcatcg
     3961 cctgaaacga aatactctga tccgcctgta tcccacccat cagtacgccg gtgtcctgct
     4021 ccgggaagaa ggttttcggg atcgagatat tcagcgcaat ggtgccaagc agcaccacgc
     4081 ccaccagacg ggtatgattg agcacccatt tcagcgactt gccgtagcct tgttgcaggg
     4141 ctaccaacat gcggccaaaa ccacgcagtc gcttttgctc acgcggcttg ctggctttta
     4201 gcatccagcc gcacatcatt ggcgttagcg tcagggaaac cagcaacgaa ataccaatgg
     4261 cgacagaaag cgtcacggca aactcgcgta acagtcggcc cggcaatccg cccatcaaca
     4321 gcagcggcag gaacaccgcc accagtgaca gactcatcga cagcaccgta aagccgactt
     4381 cgcgagtacc ttgcagcgcg gcctgtagtg gtttcatccc cgcttccaga tgacgggcaa
     4441 tgttttccag caccacaatg gcgtcatcca ccacgaaacc agtagcaatg gtgagcgcca
     4501 tcagcgaaag attattgaga ctgaatccgc acaggtacat cgccgcaaac gtaccaatca
     4561 gcgaaaccgg caccgcaacg gcgggaataa tagtggcgcg acccgagcgc aggaataaaa
     4621 agaccaccag aatcaccagc gccaccgaga taatcagcgt ttgctcgact tcttccagcg
     4681 aggcgcgaat ggtgggggag cgatcctggg caatttgcag atcaatcgcc gccggaatgg
     4741 tctcctgcaa ctctggtaat tttgcccgga tgctgtctac cgtctggata atattggctt
     4801 ccggcagttt gcggatcatc agtaaaatag ccggtttggc gttggtcatc ccggcgttgc
     4861 gcacatcctg cactgagtca gtcaccgtcg ccacatcgcc cagacgaacc gcgccgccgt
     4921 tgttgtagtg aataatcagc ggctgatatt cagcggcggt tttcagttca tcgttggtct
     4981 ggatctgcca gcggtgagtg ccatcttcca gcgccccctg cggtttacgc acgttggcat
     5041 tgctgatggc agtgcgtacg tcgtccagcg acacgccctg attaaacagc gcctgcgggg
     5101 tcagcccgac gcgcacggcg ggcagcgagc tgccaccgac atcgacatca ccgacaccgt
     5161 cgatttgcga aatcgtcgga gccagctgcg tcgaggcgaa atcgtacagt tcaccctgcg
     5221 aataagtatc ggatgtcagc gtgaggatca taattggcgc atccgacggg ttcgctttgc
     5281 gataggtcgg gcggctgggc atcccgctgg gcagcaaact ttgtgcagcg ttgatcgccg
     5341 cctgcacatc acgcgctgcg ccgttgatat cccggtcaaa atcaaactgc aaaataatgc
     5401 gcgtgctgcc gagcgaactg ctggaggtca tttcactaac cccggcaatg cgcccaagtg
     5461 agcgctccag cggcgtggca acggaagacg ccattgtctc cggcgacgca ccgggcagcg
     5521 aggcgctgac cataatcacc ggaaaatcga cctgcggcag cggggcgacc ggcagcatac
     5581 ggaaacccag tatgccgcac agggtaatgg cgaccgacag taaaatcgtc gccaccgggc
     5641 ggtaaatgaa gagggcaaaa aacttcactt acgcctcctc ttcatggcga gcaaagcgac
     5701 ttttggtcca caatgccagg cggtcgaaca gcagataaat caccggcgtg gtgaacagcg
     5761 tcagcacctg gctgacaatc agaccgccga ccatgccgat acctaacgga cgacgcagtt
     5821 ccgcgccgac cccggtactc aacatcagcg gcagcgcgcc aagcagagcc gccagggtgg
     5881 tcatcaggat cggacgaaaa cgcaacagac aagcctggta gattgcatcg cgcggcgaca
     5941 tgccttgctc gcgctcagca gccagcgcga agtcgatcat catgatggcg ttcttcttca
     6001 caataccgat cagcaaaata atgccgataa tcgcaatcac atccagttcg ctaccggcaa
     6061 tcattaatgc cagcagcgcg ccgacccctg cggtgggtag cgtcgagaga atggtgatcg
     6121 ggtgaataaa gctctcgtac agaatgccga gcacgatata catcgccacc accgccgcga
     6181 caatcagcca gacagtgctg cccagcgccg actggaaggc gagggtgctg ccctagaatt
     6241 gcgtggtgat atccaccggc agattcagcg tcttttcggt gtccataatc gcctgcaccg
     6301 catcgcccag cgaatagtta tccggcacgt taaaggagat ggttgttacc gggaactgat
     6361 ccagatggtt gatggaaagc ggcgcaaaac gctgctcaat tttggcaatt gagcttaacg
     6421 gcaccacgcc accgtcgctg ctggtcaggc gaatggtatc cagcgccgcg aggcctgggg
     6481 tattttcggt attgtgctcc agcaccacgc gatactggtt agcctgagta taaatagtgg
     6541 aaatcagtcg ctgaccaaac gcgttgtaca gggcgttatc gacgtccgcc atgctgatcc
     6601 ccagacggct ggcgctgtcg cgatcaacat tgacatacgc caccagccct ttgtcctgcc
     6661 agtcgctgga gacatcagaa agctgtggca gttgctggag tttttccatc aactgtggca
     6721 cccaggtact gagcgcatcc agtgacgtgg cctgcaaggt aaactggtac tgggtgcggc
     6781 tgacctgagt atcgatggtc aggtcctgcg ttggttgcag gaagagatcg acgcccggca
     6841 ctttatctac cgccgtttgc agacgggcga tgactttttg cacccgatca tcacgttcat
     6901 ccaacggttt gaggttgatt tgtaaacgtg cactattcag cgacgggtta gtgccatcaa
     6961 cgccaacaaa tgaggtcagg ctttgcactg ccggatcctg caaaatcacg tccgcgacct
     7021 ggcgttgtcg ctgggccata ttggcaaagg agctggattg cggtgcctgc aaagtgccct
     7081 gaataatgcc gttgtcctgt accgggaaga aacctttcgg aatgaacacc cacagcagca
     7141 cgctaagcag cagcgtgctg agtgccacgc ttaaggtcag ccacggatga ttcagcactt
     7201 tcgccagtcc acgaccatag gcggcgatta tcctgtcgaa cattttttcc gaggcacggg
     7261 agaagcggtt ctgtttacgc aacgactcct ggctgagcat ccgcgcgcac atcatcggtg
     7321 tcagggtcag cgacaccacc gctgagatca aaatcgctac cgccagggta atagcaaatt
     7381 cgcggaacag tcgcccgacg atatcgccca taaacagcag tgggatcaac accgcaatca
     7441 gtgagaaggt cagcgagata atggtaaagc cgatttcacc tgcgcccttg agcgccgccg
     7501 ccaacggttt ttcgcctttt tcgatatagc gggaaatgtt ttcgatcacc acgatggcgt
     7561 catcgaccac gaatccggtg gcgatagtta acgccatcag tgtcaggtta ttgattgaaa
     7621 aatcgagaaa caccataacc gcgaaagtgc cgattaacga cagcggtaca gcaacaccgg
     7681 gaatgatggt cgccggaata ttgcgcaaaa acaggtagat aatcatgact accagcgcga
     7741 tagccatcat caattcaaac tgagtatcat cgacggatgc gcggatattg gtggtgcgat
     7801 cggaaagcac tgtcaccttc accgatttcg gcagactctc agtgagctgt ggcagcatct
     7861 gccgaatgct gtcggcggtg gagataatgt tagcaccggg ctggcgctga acattcatca
     7921 caatggcctg ttctttgttc gcccacgcgc cgagccagct gttttctgca ccttgctcta
     7981 cagttgcgac atcgcccaga cgaattggcg cgccgttctg gtaggcgatg attagctggc
     8041 gatactcttc ggcggattgc atctggtcgt tcgcggaaag cgtgaccgca cgggaagggc
     8101 cgtcgaggct accttttgcc gagttaacgt tagcgccggt aatggcggtg cgcacggttt
     8161 cgctggtcag gccgagggcg gcaatcgcct gagcgttaag tttgacgcga acagccggac
     8221 gctgaccgcc ggaaagcgtc accaggccga cgccggaaat ctgcgagatt ttctgcgcga
     8281 cgcgggtttc caccatatct tccacttgcg tcatcggcat ggcggttgag gtgacggcga
     8341 gcgtcatgat cggcggatct gccgggttca ctttgctgta aaccggcggg ttaggcagat
     8401 cgctcggcaa caagttggtc gcagcgttaa tcgcggcctg cacttcctgc tcggcgacat
     8461 tgagcggtaa tgttagctgg aactgcaaag tgataactga cgcaccgccg gaactttgcg
     8521 acgacatctg tttcaggcca gacatctgcc cgaactggcg ttcgagcggc gcggtaacgg
     8581 cagaggtcat gacatccggg ctggcacctg ggtagagcgt gaccacctga atggtcggat
     8641 agtccacttc cggcagcgcc gaaacgggca gggcgcgata accgataatc ccggcgagta
     8701 agatcgccac catcagcagc gtggtggcga caggacgcat aataaacagg cgcgacgggc
     8761 cgcctgtgct gctcgggggt aacacctgca tcaggagcgt gctccttttt tcgcgtattc
     8821 gcggctggtg gctttctctt ccggagtggt ggcgctctgg gcttccacca cttccacttt
     8881 cgccccttcg gtcaggcgat caatgccgtc tgtcaccacg cgatcgcccg cagaaatacc
     8941 tgcacggatc accactttct gactgtcctg aatgcccggc gtcaccagat gtttgctgac
     9001 cttgttttcg ctattcagca cccagacaaa atggccttca ttgcccattt gcagggcggc
     9061 tgttgggatc actacggcgt tttgttcggt gtcgactaac atgcgcgcgt taacaaactg
     9121 attgggaaac agcgcatcat cctgattatt aaagcgtgct ttcactttaa tcgtaccggt
     9181 tgtggcatcg atttggttat cgaggcttaa cagcgtgcct tcacttaatt tcttcgaatt
     9241 ggtgcgatcc caggcttcta ccatcagcgg ttttccggct ttttgcgcct gcactacagt
     9301 agcgatatcg ctttccggca gggtaaagac taaatcgata ggatgcgtct gggtgatcac
     9361 cacaattccg gtggtatcac cactggagat ttggttacca acatcaactt gcttgagacc
     9421 aacgcgacca tcgactggtg cggtgatgcg gctccagtcg agttgcaact gcgcgctggc
     9481 gacgcttgct tcatcagcct taatggtgcc ttcggtttca ctgaccagcg cctgttgggc
     9541 atccagctcc tgacgggaga cgagattggt ttttgccagt tgctgataac gcgccagatc
     9601 acggcgggcg ttagcaagcg tggctttatc ttttgccagt tggccctggg tttgtgctaa
     9661 tgcaactttg aactggctgg ggtcaatttc tgccagtaaa tcgcctgctt tgacctgctg
     9721 gccttcctgg aaatgtaacg ccatcagttg accgtccacg cggctgcgca ccgtaacagt
     9781 attagcggcg gtaatggtgc caagccc
//
LOCUS       DAJTUN010000131         9713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTUN010000131 DAJTUN010000000
VERSION     DAJTUN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.131"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1418
                     /gene="amn"
                     /locus_tag="OO470_003298"
     CDS             <1..1418
                     /gene="amn"
                     /locus_tag="OO470_003298"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6252444.1"
                     /translation="LDKLDALYEQSVVALRNAIGKYITIGELPDENARKQGLFVYPSL
                     TVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQ
                     PSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSH
                     FDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIAL
                     SCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTIC
                     DHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEV
                     QRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDME
                     SATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLL
                     RAEGDRLHSRKLRTFNEPPFR"
     gene            1761..2477
                     /locus_tag="OO470_003299"
     CDS             1761..2477
                     /locus_tag="OO470_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6252445.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(2933..3008)
                     /locus_tag="OO470_003300"
     tRNA            complement(2933..3008)
                     /locus_tag="OO470_003300"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2973..2975),aa:Asn,seq:gtt)
     gene            complement(3109..4736)
                     /gene="yeeO"
                     /locus_tag="OO470_003301"
                     /pseudo
     CDS             complement(3109..4736)
                     /gene="yeeO"
                     /locus_tag="OO470_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4741..4816
                     /locus_tag="OO470_003302"
     tRNA            4741..4816
                     /locus_tag="OO470_003302"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4774..4776,aa:Asn,seq:gtt)
     gene            complement(4854..5804)
                     /gene="cbl"
                     /locus_tag="OO470_003303"
     CDS             complement(4854..5804)
                     /gene="cbl"
                     /locus_tag="OO470_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6252446.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5906..6823)
                     /gene="nac"
                     /locus_tag="OO470_003304"
     CDS             complement(5906..6823)
                     /gene="nac"
                     /locus_tag="OO470_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6252447.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7151..7226
                     /locus_tag="OO470_003305"
     tRNA            7151..7226
                     /locus_tag="OO470_003305"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7184..7186,aa:Asn,seq:gtt)
     gene            complement(7282..8217)
                     /gene="ldtA"
                     /locus_tag="OO470_003306"
     CDS             complement(7282..8217)
                     /gene="ldtA"
                     /locus_tag="OO470_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6252448.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8279..9358)
                     /gene="cobT"
                     /locus_tag="OO470_003307"
     CDS             complement(8279..9358)
                     /gene="cobT"
                     /locus_tag="OO470_003307"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6252449.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9370..>9713)
                     /locus_tag="OO470_003308"
     CDS             complement(9370..>9713)
                     /locus_tag="OO470_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6252450.1"
                     /translation="GEPILASLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDG
                     RQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIE
                     LGELVFLLALL"
BASE COUNT         2715 a         2446 c         2096 g         2456 t
ORIGIN      
        1 cactggataa actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg
       61 gcaaatatat tacaattggc gaattacctg atgaaaacgc ccgtaaacaa ggtctttttg
      121 tctatccatc actgaccgta acctgggacg gtagcacaac aaatcccccc aaaacgcgcg
      181 catttggtcg ttttactcac gcaggcagct acaccaccac gattactcgc cctactctct
      241 ttcgttcgta tctgaatgag caactaacgt tgctttatca ggattatggt gcgcatatct
      301 ctgtgcaacc ctcgcaacat gaaatccctt atccttatgt catcgatggc tctgaattga
      361 cacttgatcg ctcaatgagc gctgatttaa ctcgctactt cccgacaaca gaactggcgc
      421 aaatcggcga tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc
      481 attttgatgc gcgccgcgtc gatttttccc tcgcacggtt gcgccattat accggtacgc
      541 cagtagaaca ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat
      601 tcgttcgttg gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt
      661 gtgctggcgg gaactggatc actgccgaaa ccgaagcgcc agaagaagcc atttccgacc
      721 ttgcatggaa aaaacaccag atgccagcat ggcatttaat taccgctgat ggtcagggta
      781 ttactctggt gaatattggc gtgggaccgt ccaatgctaa aaccatctgc gatcatctgg
      841 cagtgctacg cccggatatc tggttgatga ttggtcactg tggcggatta cgtgaaagcc
      901 aggccattgg agattatgtc cttgcacacg cttatttacg cgatgaccac gttcttgatg
      961 cggttctgcc gcccgatata cctattccga gcattgctga agtgcaacgt gcgctttatg
     1021 acgctaccaa gttagtcagc ggcaggcctg gtgaggaagt caaacagcgg ctgcgtacag
     1081 gtactgtggt aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc
     1141 gttttaactt aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag
     1201 gatatcgttt ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttacatg
     1261 gtgagattaa gcttcccggc caggctaacc gtttttatga aggcgctatt tccgaacacc
     1321 tgcaaattgg cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac
     1381 gtaaattacg aacctttaat gagccgccgt tccgataata agttatgaac cttcttatca
     1441 gaaatagatg tgaaggatta agtaagacat ctggcaaata gcctgcaata aacgcgggct
     1501 gcttgcttcc ttgggcagat gcgagtttta ttatcgtctt aatgatttcc acatattaaa
     1561 agcaagtatg ctttcaaaac acaattataa aaaatcccgc caacaacatt agtttttata
     1621 aaattaaata taagattatg gctttagaat atttttattt ctaatagacg agatttttcc
     1681 tgttatgata taatatgccg aattaacaca tgttaacgat ttaccagtaa tgtaaataaa
     1741 ttttcgagga gatcattcca gtgggacgta aatgggccaa tattgttgct aaaaaaacgg
     1801 ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc tatgctgctg
     1861 ccaaacaagg tgaacccgat ccagaattaa acacatcttt aaaattcgtt attgaacgtg
     1921 caaagcaggc acaagttcca aagcacgtta ttgataaagc aattgataaa gccaaaggcg
     1981 gcggagatga aacgttcgta cagggacgtt atgaaggctt tggtcctaat ggctcaatga
     2041 ttattgctga gacattgact tcaaatgtta accgtacgat tgctaacgtt cgcacaattt
     2101 tcaataaaaa aggcggcaat atcggagcgg caggttctgt cagctatatg tttgacaata
     2161 cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt ttgcttgaag
     2221 ctgaagttga tgttcgtgat gtaactgaag aagaaggtaa tattgttatt tatactgaac
     2281 ctgctgacct tcataaagga atcgcggcgc taaaagcagc tggaatcact gagttctcaa
     2341 caacagaatt agaaatgatt gctcaatctg aagttgagct ttccccagaa gatttagaaa
     2401 tctttgaagg gcttgttgat gcccttgaag atgacgacga tgtacaaaaa gtttatcata
     2461 acgtcgcaaa tctctaatta tattttaaag aaatctgtct ttatggcaga tttctttaat
     2521 ctcatataat tcttataaaa aatataatat ttaactcgtc atattgctta taaaaaatcc
     2581 ccgtccccag agaaataata tttattaaaa tggcaggtct tctttttctg attaccgaag
     2641 acaaagtggc aattacgtat agtttcccga taaagacgcg acagctacgt cccgcataag
     2701 gcatttttct ctttatcttt gtacggtact tcatggaaca gaatttttga ccttgtgaat
     2761 cgtgatatct gttggggagg ggcaatttgc tcactgaagc gtgagactcg attaagcgca
     2821 cgaaacacag aaatcaaaaa acccggccac ttttttacaa agtggccggg taaaaataat
     2881 ttttattttt taactgtcct gagtctcata gagatgtctc aaaactaaaa tttggctcct
     2941 ctgactggac tcgaaccagt gacatacgga ttaacagtcc gccgttctac cgactgaact
     3001 acagaggaat cgtgagaacg aggcgaatat tagcgatgcc cacccacaat gtcaaagcct
     3061 gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt attccgacac
     3121 aactggcttt ttttcacact tttgcggctc ggatcgaggg tatttccaca gccaacgtcc
     3181 agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa acattcccat
     3241 ccagacacca accacacccc agccaagcat gattccctgc acataaccga ctacaacccg
     3301 gcaaccccac atgctcaaca tagaaaccca catggcgtaa cgggcatcac gagcaccctt
     3361 aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag catttagcta
     3421 aatcagaatc acaacgacgt gtttaacctg tggatcctgg gtgtaaaacg atgccataac
     3481 cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg acagccagaa
     3541 cacatgccgc aactgaatct ctgcttgcgc tatctgccct acccccaacc ttcggcctgt
     3601 aatgatcgta gaagcagaac cgagcgcact tccgggtaag ttgataagag ccgcaattga
     3661 gaacgcgata aaatttccgg caataacgct ggtccccatc ccggcaacga acatttgggt
     3721 taataaccga ccactggtaa ataacactga ttcgacactc gctggaatac caatccccat
     3781 gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg aaatccttag
     3841 cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa tataacgaga
     3901 aatggttaaa cccaacccag ccccgaccag gagaaaaggc cgtaaatcaa tatgccgcta
     3961 ataataatat taagaatatt caggctaccg ttaatcagta gcggtatttt cgtattccct
     4021 gcaccacgaa gtgccccgct accaataaga gtgatggcag cggctggata actgagtacg
     4081 gtcagctcca gataagtcaa cgccagtgct ttaacttctg tcgtggcatc acccgcgacg
     4141 aaatcaataa tttgttcgcc aaaatgatga attagcgttg ccaacagtac ggcaaacaac
     4201 gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg
     4261 agactaaatg ctacaacgac agtagtacca agatcgatag ctgcaaaaaa agccataatg
     4321 accatattga agctgtccgc caaccccacg ccggccatcg catcttttcc cagccaactg
     4381 accagaaaag tgctcaggcc ccccatcaac aggacacagg cgttctccat gaagatagga
     4441 acagcaagcg gggttatctc gcgctagaac aacactttgt agctcttgcg tttagagtgc
     4501 cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat
     4561 tgcagaaagt gaaaccacat ttcaaatgat gagggagaat cagcaaagct gcaaagattt
     4621 tcgccaacaa attgtctgca aatgcaacaa actgttgata gaaacggcaa acagttggga
     4681 aatttaaaaa tcgggtttga caaaatattt ttcgccgtta agatgtgcct caacagcgat
     4741 tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac tggttcgagt
     4801 ccagtcagag gagccaaatt tctgaaaagc ccgcttttat agcgggcttt ttgctatatc
     4861 tgataatcaa tttcctcttc actgttttcc atcacctggc gcttgatatc ctcaactgac
     4921 agtcctgcat tacaaaattc cagaaagcgc caaacatagt tacgctgaag ttgtcctcgc
     4981 ttcagtccca accagacagt attagcatcg aaaagatgcc gcgtatccag gcggattaaa
     5041 ttctcttcct cttgttcgcc gctggattgt tcggcaacta atccgatccc aagcccaaga
     5101 gcaacatagg ttttaatgac atcagaatcc tgtgcactta atacaatatc tgccggcaaa
     5161 cctttgcggg taaatgcgtc atcagtgcgt gagcgccccg taattccctg tcggtaagta
     5221 attaacggcc acttcgctat tgattccagc gtcaatggtg taatttgcgt taagggatga
     5281 tcgagtggaa caagcaaact atggtgccaa caaaaccacg ggaaggcgac gagctgcggg
     5341 tcattactca aacgctcgct ggcgatacca atatcagctt cgccattttg caacaacgtc
     5401 gcaatttcct gtggcgaacc ctggattagc tcgagccgaa cctccgggaa aagttcgcga
     5461 aaagctttaa tgacctctgg caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg
     5521 agaacaccag acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt actggcttca
     5581 ttcagaatac gttctgcaat gaccagtaat gctttgcccg gttcagtcat ccccagcagt
     5641 cgcttacctc gtcgaacaaa tatttcgatg ccaaattcat cctccagttc ccgaatatga
     5701 cggctaacgc ctgactgtga ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc
     5761 tgacgtgcag cctcgcggat tatctttagt tgttggaaat tcacggtaaa ctccgggcag
     5821 ttcagatatc ccgttattgt taaagtctaa tgcccggcat aacaaataat aaaaacccgc
     5881 atcttattcc atcccgatat aacgcttagc tcaccaattg ccactgcctt ttttccatca
     5941 ctggagaact aatcactgac attaacaact ctttcactgc ctgtgcctgt ggtgacaagt
     6001 ttgctctggc gggtaaattt aaggacaaag agagactcat tgaaggagtg gtaatgcgtg
     6061 acatccaccc atttactgcg ccacataacg aacgcgcagc cgattcaggt aatactgcaa
     6121 cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat agactcaatt tcaccaatga
     6181 cttttgccgt gagccgccgt agggaaaaag cctcatcaac acgaagtcta acagcgctgt
     6241 aatcactggg gagaaaaagg ttcatttgcg caatagcatt aacatcaacg ttttgccccg
     6301 ggcaatcttg agttcctacc aggaaaagat cttctttcag caaagcctga ctggataccc
     6361 cagccacagg ggaatgctca taaatcaccg ccatatcgag ttggtgattt atcagttttt
     6421 cgttaagcac tgcaccacta ttttcatgaa gatagataac gacctccggg aattcagcgc
     6481 gaaccgcctg taataagggc atggtgatgg atgacgcagc ggttcctggc gcaaagccaa
     6541 tcgagacttg ccccgataat gactgaccaa cgttatgcac cgccagttgg gcctgctcac
     6601 actgacgtaa aatggcccgt gcatgggtat agataatttt tccggcgtct gttggcgtaa
     6661 cgccccgttt tgtgcgaatc aaaagctgtt gatttaactc accttccagt atggcaacct
     6721 gctggctgag cgctggttgt gcgatatgca atacttcagc agcctgggtc aggctaccaa
     6781 tatctacaat ttttacgaag tatttcaggc gtctgaagtt catgttgcct ccggttttta
     6841 agaatcggcc caagtgccgc cattacttac aaccagtatg caagatgcct gccagttcta
     6901 ttttggcgtt gatgcgcaag ctaaccaact gtcaaataag agattatgat agattcgtca
     6961 ttggcccttt ttatcagcag tcgcattcac ctgccctata aaaggagggt atgcaccacg
     7021 atggttcatt acccaataag attgaaagct caccactttg ttgaaattgg cagcaaacaa
     7081 acaaaaaaaa tgcatttcac cctttgacat caccatgcac tgccattaat atgcgccccg
     7141 ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
     7201 tggttcgagt ccagtcagag gagccaaatt caaaaaagcc tgctttcgag caggcttttt
     7261 actttctaat taccaacgct cttaaaacat ctgtcttgaa ccagaactaa tttgcacagg
     7321 cattcccgat cgatgttgca acgcagcatt tgcgcgattt acatcaactt cttgcccgtt
     7381 gataaacgcc cgcaaagatg gggttaccgg caatggcact tttcggtcag actcatattc
     7441 tgcacgattg cgcgacagcg gctcatgaac ttccagccac tttgaaccat ccggttccgt
     7501 tgtgtatttc actggctgat caataatttg tacacgagtg cctacaggaa cattatcaaa
     7561 cagatatttg atatcgtcat tacgcagacg aatacagccc tgacttacac ggagcccaat
     7621 accaaaattg gcattggtac cgtggatggc atacaacctg ccaatataaa tcgcgtacag
     7681 ccccatgggg ttatcgggcc ccgcaggaac aaatgcgggc aaactctccc ctcgtttcgc
     7741 atattcgcgc cgggtgttcg gcgttggcgt ccaggttggt gcttcttgtt tacgttcaac
     7801 ggtagtcacc cagttacgcg gggtttctcg cccagcctgg ccgataccaa taggaaagac
     7861 ttccactgta ttactgtctg gcggatagta ataaagacgc atttcagcga cgttaaccac
     7921 aatcccttta cgaacagtgg cgggcaaaat cagttgctgc ggaatggtga gttgtgagcc
     7981 aggcttcggc aaaaaaacat cagcgcccgg attcgcttcc agcatattac ttaacccttg
     8041 cccgtattgt gcggcaaaag tctccagcgg ctgagtattg tgatcaggaa cagttacagt
     8101 aagcgactgc cccactaaac ggctaccctc tggaggtaat ggataagtta ccgccaggct
     8161 attttggctg gcaaaaagca gagcaaatga gcaaagaata tttacacgac gcatcatgtc
     8221 cctttcctaa gtcgcgaata ctatccgata agtatagctt ttatcagact tttcgttttt
     8281 agctgttcaa atcagaagtc gtattccccg gtagaacaat attactggca gcaagttcgc
     8341 ccatgttgtt gtatatcgca caagcagctt cgatgatgga catcgccaga gctgcaccac
     8401 tcccctcacc taaacgcata tccatattga gataaggctc cagccccaaa tgcgagagcg
     8461 ctatacgcgc gcctttttct gccgacaagt gagaaggaat gagatacggt ttgattgcag
     8521 gagacatctg gcaggctgcg agcgcagcag cataagaaag aaatccatcc agcaacacgg
     8581 gtaaaccgca ggaagcagca cctaacatca ctccagctat tccgaccaaa tcaaatccac
     8641 ccacttttgc caggacatca acaccatcct gaggatttgg ttgattcaac gtaatcgccc
     8701 gacgcacaac atcaatttta ttagccagtt tatctgtcgg caggtttgcg ccaatcccaa
     8761 ccacttcttc aggatcccgg ccagtgattg tgctgactat tgccgctgcc ggtgtcgtgt
     8821 ttgccatccc cagttcacct acaccaaaca gcgtgacacc gtttttcgcc agctcccgcg
     8881 tataacatat gacgtccaaa agcaactttt cagcctgacg gcgactcatt gccggagctg
     8941 aagcaatatt gccgctacct cgtgcgacac gcatgttgat aagcccgggg ataggctcag
     9001 cagtatcaat accaacatca attacgtgga cgttagcgcc cgcttgtgct gccagcacac
     9061 acacgccggt tgttccacgg gtcatatttt cagcctgtat ggctgtcact tcttttggag
     9121 aaatagcgac cccttcctcc cagacgccgt gatcggcaca cataaccagt accgcttttt
     9181 tgcccacatg cggtatgcca ttcacccccg gcattcctgc cagttgtatg gcaagcgcct
     9241 ccagctttcc taggctacca acaggtttga ataacccgtc aacatgccgt tgtgcacgcg
     9301 acatagcggc agaattgatg gctgggatcg tattcagtaa atcggcaatt atttgcatgt
     9361 cacgtttctt tatagcagtg ccagcaggaa gaccagttca ccaagctcta tggctgctcc
     9421 cagcgtatcg cccgtttgcc cgcccagcgt acgtttgaga agctgaccga ggatgaaaat
     9481 cgccaccatc gtcaccacca tagcagccac accatgcata cccagcaata atacggcagc
     9541 aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc ccatcaattt tgccaatgaa
     9601 cacattgcca agcccttctt cccgcgcata acgatgacga tacatcaata atgcggcagt
     9661 cccgcggctg accgcacatg ctgccgccag cgatgcaagg atcggttcgc cac
//
LOCUS       DAJTUN010000132         9589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTUN010000132 DAJTUN010000000
VERSION     DAJTUN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.132"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..608)
                     /locus_tag="OO470_003309"
     CDS             complement(<1..608)
                     /locus_tag="OO470_003309"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; functions in amino acid
                     biosynthesis; lysine sensitive; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6252451.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSD"
     gene            1133..2782
                     /gene="pgi"
                     /locus_tag="OO470_003310"
     CDS             1133..2782
                     /gene="pgi"
                     /locus_tag="OO470_003310"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6252452.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            3244..3486
                     /locus_tag="OO470_003311"
     CDS             3244..3486
                     /locus_tag="OO470_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252453.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            3600..4238
                     /locus_tag="OO470_003312"
     CDS             3600..4238
                     /locus_tag="OO470_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6252454.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            4235..4972
                     /locus_tag="OO470_003313"
     CDS             4235..4972
                     /locus_tag="OO470_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6252455.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            4972..7068
                     /locus_tag="OO470_003314"
     CDS             4972..7068
                     /locus_tag="OO470_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6252456.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7115..7393)
                     /locus_tag="OO470_003315"
     CDS             complement(7115..7393)
                     /locus_tag="OO470_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252457.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            7394..7537
                     /locus_tag="OO470_003316"
     CDS             7394..7537
                     /locus_tag="OO470_003316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252458.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            7663..8073
                     /gene="psiE"
                     /locus_tag="OO470_003317"
     CDS             7663..8073
                     /gene="psiE"
                     /locus_tag="OO470_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6252459.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8117..>9589)
                     /gene="xylE"
                     /locus_tag="OO470_003318"
     CDS             complement(8117..>9589)
                     /gene="xylE"
                     /locus_tag="OO470_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6252460.1"
                     /translation="NTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVAP
                     QNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGSA
                     WPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRG
                     KLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLYT
                     VPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGV
                     IVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMT
                     VDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCWV
                     LLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCM
                     GVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2413 a         2465 c         2497 g         2214 t
ORIGIN      
        1 taatcgctgc ctccacggcc aagcgtcgtt gtacgacctt tattttcgct accgataaat
       61 ccctgggtga tcactaagcc ttcattgagg cgtgggagca gctgcagcgc ggccagttcc
      121 gccagcgcgg ctacatctgg ctctgcacga ccaaatcggt cgttggtacg catcacttta
      181 cgtacatcaa accactgtgc ctgaacatcg cgttcgcgca ggatttcgac aaacagcagg
      241 gtcgacatca gctcgccgtg gctgaccagc tcatctgtca gcgccggaga cgttgccagc
      301 gccgccgctt ctgccagaac agtaatgttc tccagcagac gttcaatctc ttcacggata
      361 acgttcgggt aacgcagacg ttccagaatg gcaaactgga tgttgcgaat agcgtcgagt
      421 ttttcgaatc gctcgccaag ttccagccct tccgctaaag cgaccagcag attagtgata
      481 ccagcagaag ccgagaggac tactaaacgc acgttggcat cagaaagcac aatatcagcg
      541 ctgcggttca tggcgtcaaa atcagctacg ctggtaccgc caaatttgga gacaacaatt
      601 tcagacataa ctacctcgtg tcaggggatc cattttcagc cttggcacaa gggaagagcg
      661 gaagacgggt gggcgcagag cgatacttcg ctactatttt cacccagaag tgctccacca
      721 cttgcgaaac gcccgactgc gaacgcttct ggtgacaacc caggggattc agcccctgta
      781 gccgatgatg aacgtggcca gccgttcaat cacctcggcg atgcaccccc tcaggtgtta
      841 tcacaggact ggctcctcca acaccgttac ttgggcaacg cgcctcttct ggcctgcgct
      901 agcgcaggta gtacatttat aaataaaggg tgagcggggc ggttgtcaac gacggggtca
      961 tgcggatttt tcatccactc ctggcggtca gtagttcagc taataaatgc ttcactgcgc
     1021 taagggttta cactcaacat tacactaacg gcactaaaac catcacattt ttctgtgact
     1081 ggcgctacaa tcttccaaag tcacaattct caaaatcaga agagtattgc taatgaaaaa
     1141 catcaatcca acgcagaccg ctgcctggca ggcactacag aaacacttcg atgaaatgaa
     1201 agacgttacg atcgccgatc tttttgctaa agacggcgat cgtttttcta agttctccgc
     1261 aaccttcgac gatcagatgc tggtggatta ctccaaaaac cgcatcactg aagagacgct
     1321 ggcgaaatta caggatctgg cgaaagagtg cgatctggcg ggcgcgatta agtcgatgtt
     1381 ctctggcgag aagatcaacc gcactgaaaa ccgcgccgtg ctgcacgtag cgctgcgtaa
     1441 tcgtagcaat accccgattc tggttgatgg caaagatgtt atgccagaag tcaacgcggt
     1501 gctggagaag atgaaaacct tctcggaagc gattatctcc ggtgagtgga agggttatac
     1561 cggcaaagca atcactgacg tagtgaacat cgggatcggc ggttctgacc tcggcccgta
     1621 catggtgacc gaagcactgc gtccgtacaa aaaccacctg aacatgcact ttgtttctaa
     1681 cgtcgatggg actcacatct cggaagtgct gaaaaaagta aacccggaaa ccacgctgtt
     1741 cctggtagca tctaaaacct tcaccactca ggaaaccatg accaacgccc atagcgcgcg
     1801 tgactggttc ctgaaagcgg caggtgatga aaaacacgtt gcgaaacatt ttgcggcgct
     1861 ctcaaccaat gccaaagccg ttggcgagtt tggtattgat actgccaaca tgttcgagtt
     1921 ctgggactgg gtcggcggcc gttactcttt gtggtcagcg attggcctgt cgattgttct
     1981 ctccatcggc tttgataact tcgttgaact gctttctggc gcacacgcaa tggacaagca
     2041 tttctccacc acgcctgccg agaaaaacct gcctgtcctg ctggcgctga ttggcatctg
     2101 gtacaacaat ttctttggtg cggaaactga agcgattctg ccgtatgacc agtatatgca
     2161 ccgtttcgcg gcgtacttcc agcagggcaa tatggagtcc aacggtaagt atgttgaccg
     2221 taacggtaac gttgtggatt accagactgg cccgattatc tggggtgaac caggcactaa
     2281 cggtcagcac gcgttctacc agctgatcca ccagggaacc aaaatggtac cgtgcgattt
     2341 catcgccccg gctatcaccc ataacccgct ctctgatcac caccagaaac tgctgtctaa
     2401 cttcttcgcc cagaccgaag cgctggcgtt tggtaaatcc cgcgaagtgg ttgagcagga
     2461 atatcgcgat cagggtaaag atccggcaac gcttgactac gtggtgccgt tcaaagtgtt
     2521 cgaaggtaac cgcccgacca actccatcct gctgcgcgaa atcactccgt tcagcctggg
     2581 tgcgttgatt gcgctgtatg agcacaaaat ctttactcag ggcgtgatcc tgaacatctt
     2641 caccttcgac cagtggggcg tggagctggg taaacaactg gcaaaccgta ttctgccaga
     2701 gctgaaagat gataaagaaa tcagcagcca cgatagctcg accaatggtc tgattaaccg
     2761 ctataaagcg tggcgcggtt aatcatcatg cgtgcctgat gccgatatgt aggccggata
     2821 aggcgtttac gccgcatccg gcaaccggag cctgatgcca atatcgacat taatgcatag
     2881 tatgaggagt taaataacct taaaaacaat gccgacaata agtcggcatt gttttatcag
     2941 ataaatcccc ttgtctgtga tttaacggaa atcataccgt gaggttaatc ctaaaataga
     3001 tttttaatcg ttgtttattt cggagaatac gcagattaat tacttttgtt tttattttaa
     3061 gcttatgatt tttattgtta tttaaatata agttgaaact tatatttgat attcattcca
     3121 attatcctaa aactccatcg ctaattcccc gcgccgtaat tcgcatgctt tagttgtgta
     3181 tactcgatcc cgcccgaaat gtttttgggt aaatctccat tcattcaatg aagggaaatt
     3241 gttatgaaaa aagttctgta tggcattttt gccatatctg cgcttgcggc gacttctgcg
     3301 tgggctgcac ctgtacaggt gggcgaagcg gcagagtcgg cagcaacgtc ggtttcggcg
     3361 gggagttcct ccgcgaccag cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg
     3421 gcaaccggtg gcggtgatgg ttctaatacc gggaccatca caaccacgac caccagtacc
     3481 cagtaataaa gtatgtatcc ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat
     3541 gaagggattt accataacca cactccggtg tggttattct gcccctctgg agaagagtcg
     3601 tgaagcgacc tgcactcatt cttatctgcc tgctattaca ggcctgttca gccacgacta
     3661 aagagctggg caattcactg tgggacagtc tgttcggcac gccaggcgta cagctgacgg
     3721 acgatgatat tcaaaatatg ccctacgcca gccagtacat gcagcttaat ggcgggccgc
     3781 aattgtttgt ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc actcaggatc
     3841 aagccactct tgtcacacaa catggtcgtc tggtgaagac tttgcttggc ggcgacaacc
     3901 tgattgaagt gaataacctt gccgccgacc cgctgattaa acccgcacaa attgttgatg
     3961 gtgcaacatg gacgcgcacg atgggctgga ccgagtacca gcaggtacgc tacgccaccg
     4021 cacgctcagt cttcaaatgg gatggcaccg ataccgtcaa ggtcggcagc gatgaaaccc
     4081 tggttcgcgt gctggacgaa gaagtctcca ccgaccaggc gcgctggcat aaccgctact
     4141 ggatcgacag cgaagggcaa attcgccagt cggaacagta tctcggcgcg gattatttcc
     4201 cggtgaaaac cactctcatc aaggcggcaa aacaatgatt aaacaaacta ttgtcgcgtt
     4261 gcttttgagt gtgggagcgt catcggtctt cgcggcagga accgtcaagg tgttcagcaa
     4321 tggcagcagt gaggccaaaa cgctaacggg cgcagagcat ttaatcgatc tggtagggca
     4381 acctcgactg gcaaacagct ggtggcctgg tgcggtgatt agcgaagagc tggcaacggc
     4441 ggcagcattg cgtcagcagc aggcgttgct gacacggctg gcagaacagg gcgcagattc
     4501 cagcaccgac gatgccgccg ccattaacgc cttacgccag caaatccagg cattgaaagt
     4561 gacaggcagg caaaaaatca atcttgatcc cgatatcgtc cgcgttgccg aacgcggtaa
     4621 cccgccgttg cagggcaact acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt
     4681 gttcgggctt atcagccatc ctggcaatca gccattcact ccaggtcgcg acgtggcgag
     4741 ctatctctct gaccagagcc tgctcagcgg tgcggatcgc agctacgcgt gggtcgttta
     4801 cccggacgga cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc acgtagagcc
     4861 gatgcccggc agcattattt atgttggcct cgcagattcc gtctggagtg agacgcctga
     4921 tgcccttaac gccgacattc ttcagactct gacgcagcgg ataccccaat aatgaaaaaa
     4981 agacatctgc ttagcttact ggcgctgggc attagcacag cttgctacgg cgaaacatat
     5041 cctgcgccca ttggtccgtc gcagtcggat ttcggtggcg taggattatt acaaactccc
     5101 accgcgcgta tggcacggga aggggagttg agtctgaact atcgcgataa cgatcagtac
     5161 cgttattact cagcttcagt gcaactcttc ccgtggctgg aaacaacgct gcgctacacc
     5221 gacgtgcgca cccggcagta cagcagcgtc gaagcgttct ctggcgatca aacgtataaa
     5281 gataaagcct tcgatctcaa actgcgtttg tgggaagaga gttactggct gccgcaagtg
     5341 gcggttggtg cgcgggatat cggcggtacg gggctgtttg atgcggaata tcttgttgcc
     5401 agcaaagcct gggggccgtt cgattttacg ctcggcctgg gctggggata tctgggcacc
     5461 agcggtaatg taaaaaatcc gctctgttca gccagtgata aatattgcta tcgcgataac
     5521 agctacaaac aggcgggatc tatcgacggt agccagatgt tccacggtcc tgcctcactg
     5581 tttggcggcg tggaatacca gacgccctgg caaccgctgc gtctgaaact ggagtatgaa
     5641 ggtaataatt atcagcagga ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc
     5701 ggggcgattt atcgcgttac cgattgggcc gacgttaacc ttagctatga acgtggcaac
     5761 acctttatgt ttggcgtcac gctgcgcacc aactttaacg atctgcgccc gtcctacatc
     5821 gataacgccc gcccgcaata tcaaccgcag ccgcaggatg ccattttgca gcattcggta
     5881 gtggcgaatc agttaacgtt gctgaaatac aacgccggac ttgccgatcc gcagatccag
     5941 gcgaaaggcg atacgctgta tgttaccggc gagcaggtga aatatcgtga ttcgcgcgaa
     6001 gggatcatcc gtgctaatcg gatcgtgatg aacgatctgc cggatgggat caaaacgatc
     6061 cgcattacgg aaaatcgcct taacatgccg caagtgacga cggaaaccga tgtcgccagc
     6121 ctgaaaaatc atctcgccgg agagccgttg ggccacgaaa cgacgctggc gcacaaacgc
     6181 gtcgagccag tggttccgaa gtccaccgag cagggctggt atatcgacaa atcacgcttt
     6241 gatttccata tcgatccggt gctgaaccag tcggtcggtg gcccggaaaa cttttacatg
     6301 tatcagctgg gcgtgatggg aacggcagat ttgtggctga cggaccatct actgaccacc
     6361 ggcagcctgt ttgcaaatct tgccaacaac tacgacaagt ttaactacac caatccaccg
     6421 caggactcgc acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc
     6481 tatgtgaata acctgcaagc caactacttc cagcatctgg gcaacggctt ctacggtcag
     6541 gtctatggag gttatctcga aaccatgttt ggcggtgcgg gggccgaagt gttgtatcgt
     6601 ccgctggaca gtaactgggc gtttggtctg gatgccaact acgttaaaca gcgcgactgg
     6661 cgtagtgcaa aagatatgat gaaattcacc gactacagcg tgaaaaccgg acatctgacc
     6721 gcctactgga cgccatcttt cgcccaggac gtgttggtga aagccagcgt cgggcagtat
     6781 ctggcagggg ataaaggcgg cacgctggag atcgctaaac gctttgacag cggcgtggtg
     6841 gtgggtggct atgccacgat caccaacgtt tcgaaagagg agtacggcga aggggacttc
     6901 accaaaggcg tgtatgtctc ggtaccgttg gatctcttct cgtctggccc gacacgcagc
     6961 cgtgcggcga ttggctggac gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag
     7021 ttccagttgt atgacatgac cagcgaccgt agcgtaaatt tccgctaagt catgggaaag
     7081 gtgccagttt tcgcactcac tggcaccctc attcttaatg acctgttgag ccatcaggaa
     7141 cgcagttcac ccccggttta cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt
     7201 ccgtcgatat cggttgcagg tctggttgcc tgagcgactg ggccatcatt gggctggaga
     7261 cattgctttg cggtaggtcg gctttatcag cagtaccgag cggaccagca taagcaggaa
     7321 gaacagagac tgataacatc aaagcagcaa aataaggctt catttttacc acctttatca
     7381 ggttacgttt catttgttcc agaggaacat tgtcgatttt tcgcgcattg ctggtggctg
     7441 ggaatcacct gaatgggtga tttttgaatt accggctttg gtgcggtttg tcttgccgga
     7501 tgcgccgcca ggcgcggctt atccggccta cgggtaggta tatccggctt tggtggaggc
     7561 gcgctccaaa tccaggttga acaaaacata cacaaaaaat atagatctcc gtcacatttt
     7621 tgcgttatac aggaagctcg ccactgtgaa ggaggtactg ctatgacgtc actctctcgt
     7681 ccgcgcgtgg agtttatctc caccattttg cagaccgtac tcaatcttgg cctgctgtgc
     7741 ctcggcctga ttttggttgt cttcctcggc aaagaaacgg tgcatctggc tgatgtgctg
     7801 ttcgcgccag aacaaaccag caaatatgag ctggtagaag gactggtggt ttactttctc
     7861 tatttcgaat ttatcgcgct gattgtgaag tactttcagt ccggttttca cttcccgcta
     7921 cgttactttg tctatattgg gatcaccgcc attgtgcgct tgatcatcgt cgatcataaa
     7981 tcgccactgg atgtgttgat ctactcggct gcgatcctgt tactggtgat caccctgtgg
     8041 ctgtgtaatt cgaaacggct gaagcgggag taaaaagaca gcacgccgaa atggcgcggc
     8101 gtgctggaca ggaagattac agcgtagcag tttgttgtgt tttcttcgtt tccggttccc
     8161 agagcgcttc cagctcctca agggttttac ctttggtttc cgggacaaat ttccacataa
     8221 acagtgctgc cagaacgccc atacaaccgt aaatccagta ggagaaaccg ttgtggaaat
     8281 gggccaccag ccaggagttt ttgtccatca tcgggaaggt ccaggagacg aagtagttcg
     8341 ccagccactg ggccgccacc gcgattgcca gcgctttacc acgaatagca ttcgggaaga
     8401 tttccgacag cagtacccag cataccggac cccaggacat ggcaaaggcg gcaacataga
     8461 acagcatcga cagtagcgcc acaatacccg atgcctgagt gtaaaacgcg gtaccgaggc
     8521 taaacatacc gattgccatt ccgagtgcgc cgataatttg cagtggctta cgaccaaatt
     8581 tatccacagt cataattgcc agtacggtaa aggtgaggtt gataactccg acaataatgg
     8641 tctgcaacag cgcgatatcc gtgctggccc ccagcgtttt gaacacttcc ggcgcgtagt
     8701 acagcaccac attgatgccg acaaattgct ggaagatgga gagcataacg ccgattacaa
     8761 tcacgcccac gccaaacatc agcagacgac caccggtttt gcggccatga tccagggagt
     8821 gtttaatttc ctgtactgcc tgagttgcaa gcgtgttgcc cataattttg cgcaggatac
     8881 tttccgtctg ttcttgcttg ccgcgtgaca tcagccagcg aggactttct ggcacggtat
     8941 acagcagcat taagaacagc agtgcaggga tacattccga ggcaaacata taacgcctgc
     9001 cgtcagtatt cagccagctg gcatcaccgg aacgggcaat aaaatagttt acgcagtaaa
     9061 ctaaaagttg cccgaaaata atcgcaaact ggttaaatga aaccagtttt ccgcgaatat
     9121 gagctggagc cagttccgca atatacattg gtgaaagcat tgaggctaaa ccaacgccaa
     9181 taccgccaat aatacgataa ataacaaatt ccgggacata acctgccaga taaataggta
     9241 ctgtgttgtc cgggtttata gaggtaaaac caagttctgg ccaggcagaa cctacaccag
     9301 aaataaaaaa caggacagca gaaatcttaa gtgaatcacg acggccgaag cggttactgc
     9361 aataaccacc gagggcaccg ccgatgatgc aaccaatcag agcgctggct acgcaaaatc
     9421 ctaacaggga gttggcagcg gattcactta agttttgtgg agcaacaaag acggtattga
     9481 gtgactcaac agtaccggaa ataacggcgg tgtcgtagcc aaataataaa ccacctaatg
     9541 tagcgactaa ggtaatcgaa aatatataac tggaattata ctgggtatt
//
LOCUS       DAJTUN010000133         9497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTUN010000133 DAJTUN010000000
VERSION     DAJTUN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.133"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1695)
                     /gene="rsxC"
                     /locus_tag="OO470_003319"
     CDS             complement(<1..1695)
                     /gene="rsxC"
                     /locus_tag="OO470_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6252461.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
     gene            complement(1688..2266)
                     /gene="rsxB"
                     /locus_tag="OO470_003320"
     CDS             complement(1688..2266)
                     /gene="rsxB"
                     /locus_tag="OO470_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6252462.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2266..2847)
                     /gene="rsxA"
                     /locus_tag="OO470_003321"
     CDS             complement(2266..2847)
                     /gene="rsxA"
                     /locus_tag="OO470_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6252463.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2924..3364)
                     /locus_tag="OO470_003322"
     CDS             complement(2924..3364)
                     /locus_tag="OO470_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6252464.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3450..3665)
                     /gene="ydgT"
                     /locus_tag="OO470_003323"
     CDS             complement(3450..3665)
                     /gene="ydgT"
                     /locus_tag="OO470_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6252465.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(3938..4063)
                     /gene="blr"
                     /locus_tag="OO470_003324"
     CDS             complement(3938..4063)
                     /gene="blr"
                     /locus_tag="OO470_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6252466.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            4306..5346
                     /locus_tag="OO470_003325"
     CDS             4306..5346
                     /locus_tag="OO470_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6252467.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(5382..6383)
                     /gene="add"
                     /locus_tag="OO470_003326"
     CDS             complement(5382..6383)
                     /gene="add"
                     /locus_tag="OO470_003326"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6252468.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6487..7659)
                     /gene="malY"
                     /locus_tag="OO470_003327"
     CDS             complement(6487..7659)
                     /gene="malY"
                     /locus_tag="OO470_003327"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6252469.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(7669..9261)
                     /gene="malX"
                     /locus_tag="OO470_003328"
     CDS             complement(7669..9261)
                     /gene="malX"
                     /locus_tag="OO470_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6252470.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
BASE COUNT         2484 a         2428 c         2415 g         2170 t
ORIGIN      
        1 gcgggcgata gctgcttcaa cggcagtttt acgtggttca gcaacggttg ctgcgtcgtt
       61 agtttgctcc agttctgcct gtttcgctct ggcttgcgct ttacgggctt cccgttctgc
      121 aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct gtgtagcctg
      181 ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat ctttggctgc
      241 aggttgaacg gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc gctccagacg
      301 agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt cctgacgaat
      361 agccgcaatt tcagcttttt cctgacggaa atattgcacc aagggaatat tgctcgggca
      421 aacccacgcg caagccccac attcaatgca gtcggcaatg ttatgcgtgg tagctttatc
      481 gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatcca cagggcaggc
      541 gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc caagttcatt
      601 ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca gccatggcaa
      661 ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg ggcagaatcc
      721 ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt tgcccgggcg
      781 agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc catcaataac
      841 ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga tatcggatga
      901 acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt tagcaccgcc
      961 agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg ccagcaccgc
     1021 gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa tttcgcgtgg
     1081 ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc aatcctgcat
     1141 caaacggtca tcggcggtaa tgtacggctc gcactcagca gcgttgataa tcaacgtttc
     1201 aatcttatct ccaccaccct gcaatttaac gcctgtcggg aatccggcac cgcccagccc
     1261 ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag agcgataatc
     1321 gacccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa tcacgcttaa
     1381 ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa cggtacccga
     1441 ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct ggccgcgcaa
     1501 tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct gtttcagtgg
     1561 aataacaaaa tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg tctgggtttt
     1621 catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc tgaatgcaga
     1681 gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg ttcagatccc
     1741 atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc gtcgggcacg
     1801 gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc atgacacggg
     1861 tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag ccaatacagt
     1921 tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct tcgccatcca
     1981 gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct tcgccacctg
     2041 gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag gggcgacagc
     2101 cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt ttctcaacga
     2161 ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg ccaaacgcca
     2221 ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca acttcaccaa
     2281 accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg caatggcatt
     2341 accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg cggcgaagag
     2401 caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg actgcaagaa
     2461 attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt tggtggtgat
     2521 aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc gcaccaccat
     2581 ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc gcaggtaaat
     2641 cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag acgtcagcgt
     2701 catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt tggaaacccc
     2761 cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca gtacagttcc
     2821 gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc cgcctattat
     2881 ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga ttcacaaacg
     2941 tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt gccaacaaag
     3001 taaacagcaa ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg aatgctttta
     3061 ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca caacgccgac
     3121 gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata gcggtaataa
     3181 aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt tgaaatgttt
     3241 gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc aacagagcga
     3301 ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc gttgtggtgg
     3361 tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg gcgttattat
     3421 aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac ggacttcggt
     3481 acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg gtgatcggca
     3541 gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag atggtcgtag
     3601 agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta gtcctgaaca
     3661 gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta gtgatgataa
     3721 aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct gatggcaaat
     3781 attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc tgacaatcac
     3841 caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg aaaacatgca
     3901 cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt accgaagcac
     3961 tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga atgactgaaa
     4021 cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc cacatctctc
     4081 ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc tccagcaaga
     4141 tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc tatttttttg
     4201 acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga gcgtgggtaa
     4261 aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag cgacaacatc
     4321 cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc cctgattgcg
     4381 ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa agtaaaagcc
     4441 gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga tcccaacata
     4501 gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa agcggcgctt
     4561 gaggcgggta aatatgtggt cgttgataaa ccctttaccg tgacactgtc acaagcgcga
     4621 gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca taaccgtcgt
     4681 tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct gggtgaagtt
     4741 gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg ttggcgtgaa
     4801 cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct tgatcaggcc
     4861 attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt acggcccgga
     4921 gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg agtcatttta
     4981 cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg atcccgaggc
     5041 agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg cgagcgtctg
     5101 ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt ggaaggtgag
     5161 gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta ctatgcggcg
     5221 attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca ggcaatccag
     5281 gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac gttgtgcctt
     5341 gcgtgaaaaa tggatggtgc aatttgcacc atccattttt gttacttcgc ggcgactttt
     5401 tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca tttccagacc attaatctgt
     5461 gcctggcgga tttgctcacg ggataaccca gcagctggcg cggcaacggt atattcgtga
     5521 atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgctggcacg aatgccatgc
     5581 tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt ctgaatattg
     5641 gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat cagcgcccga
     5701 tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg aatcgcctgc
     5761 cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca gcccgcatca
     5821 cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga atccaagttc atcaccagct
     5881 aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc ttgctgacag
     5941 gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac accaaaggtg
     6001 cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac aggcagctta
     6061 tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagcgcag gccgttacgg
     6121 gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga ggcgagaact
     6181 ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt ggcaatgacc
     6241 tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa gcgagatatt atactggcgg
     6301 ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg gatatcagtt
     6361 aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa gtgcgcacca
     6421 ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta cgttgcgcaa
     6481 ttggggttag cgaacagcgc ggatggcgtt tattaatcca gccacacctt tttccagttt
     6541 cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac cataggtata
     6601 ccccggcatg atcgcgactt tttcttgctc gataagcgct ttttgcaacg cgttgtcgtc
     6661 aatattcaac ggacgtagat caagccaggc cagataagtg gattgcggga tctgccagtt
     6721 gagttcagga aacgcggcgt tcattttatc tgagatatac gtcaggttat ctttcagata
     6781 gacgcgtaag gcatccagcc acggtgcacc ttgctgatag gcggcgatat gggcagttaa
     6841 cgccagtacc gaaggggaag aaagcccatc acgtcctttc agtgccgata aataggcatc
     6901 acggctgctg ctattttcta taatcccgta agcaccggtc agcgcgggaa tattgaaact
     6961 tttcgatccc gatgtcagca acgcccagtc tccccgcgcc acattactcc agggaatatg
     7021 cggctgctcg ccccagacca tatccatatg gatttcatcg gaaataaccc gcacatcatg
     7081 acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt tcccggtagg
     7141 attctgtggg ttacacagga gcataatttt acattctggt ttcgccaaaa cggcttccag
     7201 cttgcccata tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg gcattactgt
     7261 gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggcgtgt ggatcaccac
     7321 gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga tgacagaagg
     7381 gccatacacc accgtctgcg tatcaattgc ggtgtaatgc tgggtggaaa accagtgggc
     7441 aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc cgtgcatcag
     7501 gcgctgattc agcgcctcga taatgcaggg ggcagtggca aaatccatgt ctgaaatcgt
     7561 gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact gtgtacacca
     7621 tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt atgcctggac
     7681 agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc cgataacaac
     7741 ctgcaggtta tgttgattaa gttgtactac gccaattgcc cgattgtcct tcagtgcctg
     7801 cacattaaca agcgacatat cttccacaga caaacgcaga cgggtaatgc agttatcgag
     7861 gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt tgtaacctga
     7921 tttacccggc gcaccggcaa cggctttttc gattgagctg gcaacttcgc tatcgcgccc
     7981 cggggttttc agattgaagc gggtgatagc gaaacggaag atgacgtagt aaacgacaaa
     8041 ccagattgcc gccaccactg gcaccatgta ccacttggtt gacagaccat gcaaaatacc
     8101 gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc cgagcacaga
     8161 catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa ctggcgctac
     8221 gaacaggaac aggaattcca gcggttcggt agtgccgcca acgacgcagg cgatcaggcc
     8281 agaaatcagc agacctttaa ttttatggcg attttccggg cgcgcgcagt gatacatagc
     8341 taacgctgca cctggtagac cgccgagaaa cgcaggcatt ttaccttgcg aaaggaaacg
     8401 cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct ggaagatggt
     8461 cagtgcgccg ctgacggttt gaccgcagac ctcctgcgtg ccgcctgcgt cggtaaagcg
     8521 aattaatgcc accagaatgt gatgcagacc aaacggcaac agcagacgtt caccggtacc
     8581 aaacagcatc ggtccgaaat cacccgcgct gtttatcata tggcccaagc cgctaatacc
     8641 catggcgaaa atcggccaga ctaatggaat caccaggccg acaaggccca tcaccagcga
     8701 ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg gcaggcggat
     8761 attatggaaa cgctcatgca gcatccagac gataataccg gcgatcaccg caccgaggat
     8821 cccggtatcg atcgactgga tcccaagaat gctctggatg ttattggctt tcagaacggc
     8881 ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag aagtttaccg cgaggttcat
     8941 taccgcataa ccgacgaagc cagcgaatgc cgctacgcct ttattttcgc gtgccaggcc
     9001 cagcgggatg gcgatacaga acatgacagg caggaaacta aaagcaaacg agccaatctt
     9061 actcatccag gtaaagatag cctgcaacac ggggttgccc aggaccggga tcagggttat
     9121 gacatcatgg ctgctaagag aactaccaat gccgagcata atgccgcaga acgacaataa
     9181 tgccacgggt aacatgaagg ttttgcctaa ctgctggaag aactcccaca gcgtgacttt
     9241 cggtgctgtt ttcgccgtca taaaacgact cctcgtagag aacaatctgg attcagtaaa
     9301 ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag tcataaatca cattacgcaa
     9361 cgataatagc gggtataaga taaataaaag gtaaaacgtt ttatctgtca cataatcagg
     9421 gagtaggtca tctgcatggc taccgccaaa aaaataacca ttcatgatgt tgcgctggct
     9481 gcgggcgtgt cggtaag
//
LOCUS       DAJTUN010000134         9402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTUN010000134 DAJTUN010000000
VERSION     DAJTUN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.134"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..407)
                     /locus_tag="OO470_003329"
     CDS             complement(<1..407)
                     /locus_tag="OO470_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6252471.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMN"
     gene            complement(599..2881)
                     /gene="pflB"
                     /locus_tag="OO470_003330"
     CDS             complement(599..2881)
                     /gene="pflB"
                     /locus_tag="OO470_003330"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6252472.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(2936..3793)
                     /gene="focA"
                     /locus_tag="OO470_003331"
     CDS             complement(2936..3793)
                     /gene="focA"
                     /locus_tag="OO470_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6252473.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4199..5959)
                     /locus_tag="OO470_003332"
     CDS             complement(4199..5959)
                     /locus_tag="OO470_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCR6252474.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6089..6715
                     /locus_tag="OO470_003333"
                     /pseudo
     CDS             6089..6715
                     /locus_tag="OO470_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            6914..8002
                     /gene="serC"
                     /locus_tag="OO470_003334"
     CDS             6914..8002
                     /gene="serC"
                     /locus_tag="OO470_003334"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6252475.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8073..9356
                     /gene="aroA"
                     /locus_tag="OO470_003335"
     CDS             8073..9356
                     /gene="aroA"
                     /locus_tag="OO470_003335"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6252476.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2390 a         2251 c         2364 g         2397 t
ORIGIN      
        1 tcgttcatct gtttgagatc gagcattacc aggtcggtta cttccagcag ttcatcaatc
       61 accggatcgt aacgacgaac aaaaccgttg gtatccagac aggtatgaat gccttctttt
      121 ttacaggcgc ggaaccagtc acgaacaaac tcagcttgca ggattgcttc accgccggat
      181 gcggtaacgc cgccgccgga agcgttcata aagtggcgat aggtcaccac ttccttcatc
      241 aaatcttcaa cggtaacttc tttaccgcca tgcgtgtccc aggtgtcgcg gttatgacaa
      301 tacaggcagc gcatcaggca gccctggaaa aaggtgataa agcgaatacc tgggccgtct
      361 acggttccac aggattcaaa ggagtgaatg cgaccaataa ctgacattgc ggtgtttctc
      421 cagatgtggc ccatctgagg ccgtgttggt gcgcagctcg aaggctacgt cgagtctgtt
      481 ttggcagtca ccttaaagta tagatagctg acaaaaaagg ctctcgtgct aaaaaaggcc
      541 ccactttcgt ggagccttta ttgtacgctt tttactgtac gatttcagtc aaatctaatt
      601 acatagattg agtgaaggta cgagtaataa cgtcctgctg ctgttcttta gtcagcgagt
      661 tgaaacgtac tgcgtagcca gatacacgga tggtcagctg cggatatttt tccgggtttt
      721 ccatcgcgtc gagcagcatt tcacggttca tcacgttaac gttcaggtgc tgaccacctt
      781 cgatggatgc ttcgtggtgg aagtaaccat ccatcagacc agccaggttg gtcttacgaa
      841 cttcgtcgtc tttacccagt gcgttcggaa cgatagagaa ggtgtaggag ataccatctt
      901 tagcgtaagc aaacggcagt ttagcaacgg aagtcagaga cgctacagca cctttctggt
      961 cacgaccgtg catcgggtta gcacccggtc cgaacggcgc gccagcacga cgaccgtctg
     1021 gggtgttacc agttttctta ccatacacaa cgttagaagt gatggtcaga acagactgag
     1081 tcgggatagc gtcacggtag gtgtgcagtt tctgaatttt cttcatgaaa cgttctacca
     1141 ggtcaacagc caggtcatct acacgcggat cattgttacc aaactgcggg tattcgcctt
     1201 cgatttcgaa gtcgatagcc agaccgtctt cgtcacgaat cggtttaact ttcgcatatt
     1261 tgattgcaga cagggagtca gcagcaacgg acagaccagc gataccacac gccatggtgc
     1321 ggataacgtc acggtcgtgc agcgccatca gagaggcttc gtagctgtac ttgtcgtgca
     1381 tgtagtggat gatgttcagt gcagtgatgt actgtttagc cagccagtcc atgaagtgat
     1441 ccatgcgctc catcacttca tcgtagctca ggagatcgcc tttgatcggt tcagacttcg
     1501 gaccaacctg cattttcagt ttttcgtcaa cgccgccgtt gattgcgtac agcatggttt
     1561 tcgccaggtt tgcacgcgca ccgaagaact gcatttgttt accaacgatc atcgggctta
     1621 cgcagcaagc gatagcgtag tcatcgttgt tgaagtccgg acgcatcagg tcatcgttct
     1681 catactgcag agaagaggtg tcgatggaca ctttagcggc gaatttcttg aagttcagcg
     1741 gcagtttttc agaccacaga atggtcatgt tcggttccgg agacggcccc atagtgtaca
     1801 gggtgttcag gaaacggaag ctgtttttgg taaccagggt acgaccatcg aggcccatac
     1861 caccgataga ttcggttgcc cagattgggt cgccagagaa cagttcatcg tattccggag
     1921 tacgcaggaa gcgaaccata cgcagtttca tgaccaggtg gtcaaccatt tcctgcgctt
     1981 cttgttcggt gatcttgcca gctttcaggt cacgttcgat gtacacatcc aggaaggtgg
     2041 aggtacgacc gaaggacatt gcagcaccgt tctgagactt aacagcagcc aggtagccga
     2101 agtaagtcca ctggatagct tcctgagcgt tggtagccgg accagagatg tcgtagccgt
     2161 atttcgcagc catttctttc atctgaccca gagcgcggtg ctgttcagcg atttcttcgc
     2221 gcagacggat agtctgttcc aggtttacgc cgttttccag atcagcctgt agagaggtga
     2281 actgagcgta tttgtctttc atcaggtagt cgataccgta cagcgcaacg cgacggtagt
     2341 caccgatgat acggccacgg ccataagcat ctggcagacc ggtcagaaca ccggatttac
     2401 ggcaacgcag gatgtccgga gtgtaaacgt cgaacacgcc ctggttgtga gttttacggt
     2461 attcagtgaa gatttttttg atcatcgggt ccagttcgcg gttgtacgct ttgcaggaac
     2521 cttcgatcat tttgatacca ccgaacggga taagagcacg tttcagcgga gcttcagtct
     2581 gcagaccaac aactttttcc aacgctttat tgatgtagcc agcgtcgtga gaggtgatgg
     2641 tggaagcaac agcggtgtca aaatcaactg gcgcgtgagt gcggttttcc agtttaacac
     2701 cttccattac tttgtcccac agggtggtgg tcgcttcagt agcgccagcc aggaaggact
     2761 cgtcaccctc gtacggcgtg tagtttttct gaatgaagtc acggacgttt acttcattct
     2821 gccagtcacc tttggtaaaa ccttcccagg ctgtggctaa cttttcatta agctcggaca
     2881 tgtaacacct accttcttaa gtggattttt tatttactgc gtacttcgac aaccattaat
     2941 ggtgatcgtt ttcacgcagg taaatgaccc agtatgtcaa cccaaccaac aaaccaccgc
     3001 cgataatatt accgatcgta accgggatca ggttatcagt gatgaagttc atcacggtca
     3061 ggtgagaaaa attttccggt gcagaaccga cagcggtcca gaattccgga gatgcgaagt
     3121 cgcggattac aatacccatc gggatcataa acatgtttgc gatactgtgc tcaaaaccgc
     3181 tggcaacaaa catcgcgacc ggcagcacca taatgaacgc tttgtccatc aggctgcggc
     3241 cagaataact catccatact gccagacata ccatcaggtt tgccaggata ccaagacaga
     3301 cggcctcaat aaaagtatgg tgcactttgt ggtcggcggt ttgtaggacg tttagtcccc
     3361 attgaccatt tgcggtcata tactcgccgg aaagccacat taaaagtaca aacagcagtg
     3421 cgccgaccag gttgccaaaa tagacattta gccagttttt agccaactga caccaggtga
     3481 tgcgcccact cgccttagca acaacaatca acactgtgga agtaaagaga tcggctccgc
     3541 agacaacaca aagaatcagc cccagagaga agcaaatgcc gccaaccagt tttgccatgc
     3601 cgaagggcat tgtgcctgtg ccagtggttg ctgtgatata gaagacgaat gcgattgaga
     3661 tgaaaacacc ggcggtaatc gccagataga aagtcttaag cggatgtttc gttgctttat
     3721 agacacccgc ctcttcggcc actttggcca ttgcagcagg aagtaaaaga tcaaaagggt
     3781 tgtcagcttt cacactaact ctctctttat taagtcggcg acgagatact aacaaagcat
     3841 tatagatgag aaattgatat agatcatatc tcgcctggct tataggcccg taactcgcat
     3901 ggtttttatg caaatacgga gtaactattt gattatccaa ataaaaataa attttaaaaa
     3961 ttaacaaatg agttgaattt tttccgcatc ctccgctaaa acagttaatt aaaagggagc
     4021 atcaggcgaa taaagtaaca atatcgatcg tatttattaa atacaaatta ccgatattta
     4081 accttataat tacaattatt ttattaatgc aaatatatgt aaagcggggc attaaaaaaa
     4141 cgccccgtaa tataactcag actaatcatt aagcctacat tgcgtaggct atttgatttt
     4201 attttgccca gaatgctgct ttggcgcgct gcagcttttc gtaggccttc aacaacgact
     4261 gatgtgcagc aaacgcgtgc agatcgctat ctaccggttg caggccgtaa aacgccgctt
     4321 cgccgctcat tgccgcactg gcggcttcta cggcatctgc gccgtacatg cgaacaaacg
     4381 cattcagata ttgcagcggc tggcgatctt cttcctgtgc cagtaataac aacgtttgca
     4441 ggcagcgata atagttggcg cgttccggac taaatactga tgagttaaac tccatcgtcc
     4501 attcggtcca gaccagagcc tgttccagat cgccaccagc cagcgccagc atggctttta
     4561 attcaccgat acgcagggtg taccaaccgt tatccgaccc ggtcgccaga cccaacagct
     4621 cacgcacgcg ggtaaagtca tcaaaatctt cttcatccag ttgctcgatg aggttcaggt
     4681 aatcttcttt ttcccactcg ctgcctggta gcgaaagaat cgtttcacgt aaatggctgc
     4741 ccatactgtt attcgcgagc cacagatctt cagccggata aatatcggac atgccaggca
     4801 cgataatacg gcaagcataa acgcccagat gctcgtaatc ggcaatataa acttctttat
     4861 cttctttgtt gaagatagcc atcagcgtag caaactcttc ttccgtggtg ccggagaaat
     4921 tccagtccac aaacggataa tcggcatcct gcttgaacag gtcccaggag attaaaccgc
     4981 tggaatcgat aaagtgcgtt tcgaggttgg tatgttcagc gacttcttca tcatcgaagg
     5041 ttggcggagt aaacacatcc aaatctttca ggccacgacc ttgcagcagc tcggtcacgg
     5101 tacgttccag tgctacgcca aaatcaggat gcgcaccgaa agaggcaaag caggtgccgt
     5161 ttgcaggatt aaacagtacc acgcaaatca ccggatactg gccgccaagg gaaccatcat
     5221 atgcgaagat cgggaaacct tctgtttcca gtgtttcgat cgcttcaacc accgctgggt
     5281 aacgcgccag cacgtctgcc gggatttccg gcaagctgat gctttcagca ataatgcggt
     5341 ttttcacgta gcgttcgaaa acttcggaca acccctgaac gcgtgcttcg ttgcgggtat
     5401 tgccagcgga cataccgtta gaaacgtaca ggttaccaat gatattcatc ggaatataaa
     5461 cggtctgatt gtcggactgg cgcgtaaacg gcagaccgca aataccacga tcttcgttac
     5521 cggattgtag gtcaatcagc atactgccgg tcaattcatt ctccggatcg taaaatgcgc
     5581 gcagacggtc atcgagcagc ccttctggca catcgtcatt ttcgctcagt gggaaccatt
     5641 tttcgttggg ataatgcaca aatggaccgt tggcgatggt ttcgcccagc cagaaatcag
     5701 caaaaaagta gttggttgag agacgctcga aatattcacc gagtgcagaa gccagcgccg
     5761 ctttcttggt tgcgccttta ccgttggtaa aacacagtgc gcactctttg tcgcgaatat
     5821 gtacagacca gacgttaggc acgggattca gccaggaggc ctcttcaatc tgaaagccga
     5881 ggtctgaaag tttttgctgg aagcgagcga tggaatcttc cagagcggca tctttgccgg
     5941 ggataaatgt ttgcgtcatg aaaatcactt tagtcgtacg gaaagcgcgc aataatacgg
     6001 gttttatctc aaaggcgcta tcaccgccgc catgccgatg aactgttgac tatgctttta
     6061 gcggataaac cacgttaaga gcataaaaat gaaggcgttc gatctccacc gtatggcatt
     6121 tgataaagtg ccttttgatt tccttggcga agttgcacta cgtagtcttt atacctttgt
     6181 actggttttt tgttcctcaa aatgaccgga agacgcggtg tgcgacagat gtcgctgttt
     6241 gaagtgttaa tcattctcac gctgggttca gcggcgggag atgtggcgtt ttatgatgat
     6301 gtgccgatgg tgccggtact tatcgtcttt atcactctgg cgttgttata ccgcctggta
     6361 atatgatggc gagctggcct ggtcgaaact caataactcc aacatgacgg aatttgagtt
     6421 ctttatggag ctacgattgc gtggcgtgga gcagctgggg caggtacgtc tggcgattct
     6481 cgaaaccaac gggcaaatca gtgtctattt ctttgaagat gacaaggtga aaccgggttt
     6541 acttatttta cccagtgatt gtacgcagcg ttacaaagtg gtgccggagt cggcggacta
     6601 tgcctgcatt cgttgtagtg aaatcattca tatgaacgcg ggggaaaaac aattatgtcc
     6661 gcgctgtgca aatccagaat ggacgaaggc aagtcgggca aaacgggtga cctgacagta
     6721 aaaacatcgg ctttttgcta ataatccgag agattctttt gtgtgatgca agccacattt
     6781 ttgccctcaa cggttttact cattgcgatg tgtgtcactg aatgataaaa ccgatagcca
     6841 caggaataat gtattacctg tggtcgcaat cgattgaccg cgggttaata gcaacgcaac
     6901 gtggtgaggg gaaatggctc aaatcttcaa ttttagttct ggtccggcaa tgctaccggc
     6961 agaggtgctt aaacaggctc aacaggaact gcgcgactgg aacggtcttg gtacgtcggt
     7021 gatggaagtg agtcaccgtg gcaaagagtt cattcaggtt gcagaggaag ccgagaagga
     7081 ttttcgcgat cttcttaatg tcccctccaa ctacaaggta ttattctgcc atggcggtgg
     7141 tcgcggtcag tttgctgcgg taccgctgaa tattctcggt gataaaacca ccgcagatta
     7201 tgttgatgcc ggttactggg cggcaagtgc cattaaagaa gcgaaaaaat actgcacgcc
     7261 taatgtcttt gacgccaaag tgactgttga tggtctgcgc gcggttaagc caatgcgtga
     7321 atggcaactc tctgataatg cggcttatat gcattactgc ccgaatgaaa ccatcgacgg
     7381 tatcgccatc gacgagacgc cagacttcgg caaagatgtg gtggtcgccg ccgacttctc
     7441 ttcaaccatt ctttcccgtc cgattgacgt cagccgttat ggtgtaattt acgctggcgc
     7501 gcagaaaaat atcggcccgg ctggcctgac aatcgtcatc gttcgtgaag atttgctggg
     7561 caaagcgaat atcgcgtgtc cgtcgattct ggattattcc atcctcaacg ataacggctc
     7621 catgtttaac acgccgccga catttgcctg gtatctatct ggtctggtct ttaaatggct
     7681 gaaagcgaac ggcggtgtag ctgaaatgga taaaatcaat cagcaaaaag cagaactgct
     7741 atatggggtg attgataaca gcgatttcta ccgcaatgac gtggcgaaag ctaaccgttc
     7801 gcggatgaac gtgccgttcc agttggcgga cagtgcgctt gacaaattgt tccttgaaga
     7861 gtcttttgct gcaggccttc atgcactgaa aggacaccgt gtggtcggcg gaatgcgcgc
     7921 gtctatttat aatgccatgc cgctggaagg cgttaaagcg ctgacagact tcatggttga
     7981 gttcgaacgc cgtcacggtt aatgccgaaa ttttgcttaa tccccacagc cagcctgtgg
     8041 ggtttttatt tctgttgtag agagttgagt tcatggaatc cctgacgtta caacccatcg
     8101 ctcgtgtcga tggcactatt aatctgcccg gttccaagag cgtttctaac cgcgctttat
     8161 tgcttgcggc attagcacac ggcaaaacag tattaaccaa tctgctggat agcgacgacg
     8221 tgcgccatat gctgaatgca ttaacagcgt tagggttaag ctatacgctt tcagccgatc
     8281 gtacgcgttg cgaaattatc ggtaacggcg gtccattaca cgcagaaggt gccctggagt
     8341 tgttcctcgg taacgccgga acggcaatgc gtccgctggc ggcagctctt tgtctggata
     8401 gcaatgatat tgtgctgacc ggtgagccgc gtatgaaaga gcgcccgatt ggtcatctgg
     8461 tggatgcgct gcgcctgggc ggggcgaaga tcacttacct ggaacaagaa aattatccgc
     8521 cgttgcgttt acagggcggc tttactggcg gcaacgttga cgttgatggc tccgtttcca
     8581 gccaattcct caccgcactg ttaatgactg cgcctcttgc gccggaagat acggtgattc
     8641 gtattaaagg cgatctggtt tctaaacctt atatcgacat cacactcaat ctgatgaaga
     8701 cgtttggtgt tgaaattgaa aatcagcact atcaacaatt tgtcgtaaaa ggcgggcagt
     8761 cttatcagtc tccgggtact tatttggtcg aaggcgatgc atcttcggct tcttacttcc
     8821 tggcagcagc agcaatcaaa ggcggcactg taaaagtgac cggtattgga cgtaacagta
     8881 tgcagggtga tattcgcttt gctgatgtgc tggaaaaaat gggcgcgacc atttgctggg
     8941 gcgatgatta tatttcctgc acgcgtggtg aactgaacgc tattgatatg gatatgaacc
     9001 atattcctga tgcggcgatg accattgcca cggcggcgtt atttgcaaaa ggcaccacca
     9061 cgctgcgcaa tatctataac tggcgtgtta aagagaccga tcgcctgttt gcgatggcaa
     9121 cagaactgcg taaagtcggc gcggaagtgg aagaggggca cgattacatt cgtatcactc
     9181 ctccggaaaa actgaacttt gccgagatcg cgacatacaa tgatcaccgg atggcgatgt
     9241 gtttctcgct ggtggcgttg tcagatacac cagtgacgat tcttgatccc aaatgcacgg
     9301 ccaaaacatt tccggattat ttcgagcagc tggcgcggat tagccaggca gcctgaatga
     9361 acaacgggca ataaatagcc aaatctttct ttatcaaaac gt
//
LOCUS       DAJTUN010000135         9275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTUN010000135 DAJTUN010000000
VERSION     DAJTUN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.135"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..268
                     /gene="mliC"
                     /locus_tag="OO470_003336"
     CDS             <1..268
                     /gene="mliC"
                     /locus_tag="OO470_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6252477.1"
                     /translation="QIQVERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLK
                     QGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            327..983
                     /gene="pdxH"
                     /locus_tag="OO470_003337"
     CDS             327..983
                     /gene="pdxH"
                     /locus_tag="OO470_003337"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6252478.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1112..2386
                     /gene="tyrS"
                     /locus_tag="OO470_003338"
     CDS             1112..2386
                     /gene="tyrS"
                     /locus_tag="OO470_003338"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6252479.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2445..3308
                     /gene="pdxY"
                     /locus_tag="OO470_003339"
     CDS             2445..3308
                     /gene="pdxY"
                     /locus_tag="OO470_003339"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6252480.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3352..3957)
                     /gene="gstA"
                     /locus_tag="OO470_003340"
     CDS             complement(3352..3957)
                     /gene="gstA"
                     /locus_tag="OO470_003340"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6252481.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4063..5565)
                     /gene="dtpA"
                     /locus_tag="OO470_003341"
     CDS             complement(4063..5565)
                     /gene="dtpA"
                     /locus_tag="OO470_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6252482.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6176..6811)
                     /gene="nth"
                     /locus_tag="OO470_003342"
     CDS             complement(6176..6811)
                     /gene="nth"
                     /locus_tag="OO470_003342"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6252483.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6811..7506)
                     /gene="rsxE"
                     /locus_tag="OO470_003343"
     CDS             complement(6811..7506)
                     /gene="rsxE"
                     /locus_tag="OO470_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6252484.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7510..8130)
                     /gene="rsxG"
                     /locus_tag="OO470_003344"
     CDS             complement(7510..8130)
                     /gene="rsxG"
                     /locus_tag="OO470_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6252485.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8134..9192)
                     /gene="rsxD"
                     /locus_tag="OO470_003345"
     CDS             complement(8134..9192)
                     /gene="rsxD"
                     /locus_tag="OO470_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6252486.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2386 a         2321 c         2352 g         2216 t
ORIGIN      
        1 gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg atgaaaaacc
       61 gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact
      121 actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg ggatctatgt
      181 tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg tcttgaataa
      241 ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac aatagcgtca
      301 cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc
      361 atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc gccgatccat
      421 taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg gaccctaccg
      481 cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc gttttactca
      541 aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc
      601 aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg
      661 tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata
      721 gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg
      781 cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag
      841 tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt gagttctggc
      901 agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat gatgcgtgga
      961 agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc
     1021 tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa
     1081 aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt
     1141 gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc
     1201 gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg
     1261 gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca agccggttgc
     1321 gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa
     1381 gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc
     1441 gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt
     1501 cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca
     1561 gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt
     1621 cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta
     1681 cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga
     1741 cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcaccaa
     1801 agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa
     1861 aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg
     1921 cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag aagaagaaga
     1981 taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgactcgtct
     2041 ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg
     2101 ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat
     2161 ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg aactgcaacc
     2221 ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa
     2281 acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt
     2341 actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa
     2401 agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc
     2461 tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccga
     2521 tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct aatcacaccc
     2581 aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag
     2641 gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc tatctgggat
     2701 cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca gcgaatccgc
     2761 aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac
     2821 cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga
     2881 atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc
     2941 tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa cacctggcgc
     3001 gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagtctggc
     3061 atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga
     3121 cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag gccctggaac
     3181 atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc
     3241 tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa
     3301 agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct attactttaa
     3361 gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc
     3421 cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagaaccgt
     3481 aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag
     3541 tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg gtttgtactc
     3601 ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg tggcgatgta
     3661 attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg
     3721 gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca gcaaagtacc
     3781 atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt aatcgtcacc
     3841 gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct ttccgctctc
     3901 acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca atttcatcgg
     3961 taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg
     4021 ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta cagtagcttt
     4081 cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg cggtcagcag
     4141 catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc gaccatagac
     4201 ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac ccgcaacata
     4261 accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat
     4321 cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc
     4381 cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga tacctgcgtc
     4441 agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatgcc
     4501 gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga tagcggccag
     4561 aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac
     4621 ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca gtgacgttgg
     4681 catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga aggcaacgat
     4741 cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga taaccacaat
     4801 accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca gtagccaggt
     4861 ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac ggtagttgac
     4921 tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt
     4981 aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt
     5041 cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga cggacatgta
     5101 gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg tagaaagcag
     5161 agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata
     5221 aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat
     5281 agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat
     5341 agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc
     5401 agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc
     5461 aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc
     5521 gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt
     5581 tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt
     5641 gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc
     5701 tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta
     5761 tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat tgagaggggg
     5821 atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga gatagtccag
     5881 tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt gtgtaaattt
     5941 aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg tgatcttgat
     6001 cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatcc
     6061 gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta aaaagatgtt
     6121 ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct tttcttcaga
     6181 tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca cagcggggct
     6241 tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa
     6301 actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt ttccccggcg
     6361 caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc
     6421 cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc agggcttcaa
     6481 gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata cggcaggttt
     6541 tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag gttttcaccc
     6601 cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag agtttcgccg
     6661 tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca atcagcaatt
     6721 caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag
     6781 tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa
     6841 tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa tcaggtattt
     6901 tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg ccagcaggaa
     6961 aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac ctaacagcgc
     7021 atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa
     7081 catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa gcgccggacc
     7141 ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga caatcagcgg
     7201 aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac
     7261 agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct cggctggcgt
     7321 ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag
     7381 tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac aaaggccgag
     7441 cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc
     7501 gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc
     7561 gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt
     7621 gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat ccgcaccact
     7681 gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga ttttatcgcc
     7741 aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc cattaaaatc
     7801 ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac
     7861 ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa
     7921 ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc gttcggctgg
     7981 cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa ttgtcgtttt
     8041 ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata gtgccagtgt
     8101 aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg
     8161 cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc
     8221 cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgccagcgc
     8281 gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag tcaaaataaa
     8341 gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg ccagtgtttc
     8401 tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga agctgagggg
     8461 aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca gccaggcgag
     8521 atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat attgcataat
     8581 ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg gtgtcgcctg
     8641 actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg cggtatgacc
     8701 gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg
     8761 cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat
     8821 tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga tgatcaccgc
     8881 aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac ttaccgccag
     8941 caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg actgcttgcg
     9001 tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca ggatctgaac
     9061 gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg ctgcgagcaa
     9121 caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat
     9181 tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt tggcctgaac
     9241 gcgggcaata gccgcggcaa ctgcggcttt gcgcg
//
LOCUS       DAJTUN010000136         9166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTUN010000136 DAJTUN010000000
VERSION     DAJTUN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.136"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(169..1146)
                     /gene="epmA"
                     /locus_tag="OO470_003346"
     CDS             complement(169..1146)
                     /gene="epmA"
                     /locus_tag="OO470_003346"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6252487.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1471..3279
                     /gene="frdA"
                     /locus_tag="OO470_003347"
     CDS             1471..3279
                     /gene="frdA"
                     /locus_tag="OO470_003347"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6252488.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3272..4006
                     /gene="frdB"
                     /locus_tag="OO470_003348"
     CDS             3272..4006
                     /gene="frdB"
                     /locus_tag="OO470_003348"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6252489.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4017..4412
                     /gene="frdC"
                     /locus_tag="OO470_003349"
     CDS             4017..4412
                     /gene="frdC"
                     /locus_tag="OO470_003349"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6252490.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4423..4782
                     /gene="frdD"
                     /locus_tag="OO470_003350"
     CDS             4423..4782
                     /gene="frdD"
                     /locus_tag="OO470_003350"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6252491.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4845..5978
                     /locus_tag="OO470_003351"
     CDS             4845..5978
                     /locus_tag="OO470_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6252492.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6067..6600
                     /gene="blc"
                     /locus_tag="OO470_003352"
     CDS             6067..6600
                     /gene="blc"
                     /locus_tag="OO470_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6252493.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6597..6914)
                     /gene="sugE"
                     /locus_tag="OO470_003353"
     CDS             complement(6597..6914)
                     /gene="sugE"
                     /locus_tag="OO470_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6252494.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7090..7236)
                     /gene="ecnB"
                     /locus_tag="OO470_003354"
     CDS             complement(7090..7236)
                     /gene="ecnB"
                     /locus_tag="OO470_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6252495.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7347..7472)
                     /gene="ecnA"
                     /locus_tag="OO470_003355"
     CDS             complement(7347..7472)
                     /gene="ecnA"
                     /locus_tag="OO470_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6252496.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7524..8090)
                     /gene="efp"
                     /locus_tag="OO470_003356"
     CDS             complement(7524..8090)
                     /gene="efp"
                     /locus_tag="OO470_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6252497.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8132..9160
                     /gene="epmB"
                     /locus_tag="OO470_003357"
     CDS             8132..9160
                     /gene="epmB"
                     /locus_tag="OO470_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6252498.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2288 a         2378 c         2371 g         2129 t
ORIGIN      
        1 actgcgcaac attgagcagg cagcgtaaaa accaatacag aattgatact tggatgatat
       61 caaatatcca gtcttaaatt gtggccttta acggaaaaat gaaaacatat gctgcagaat
      121 ggcgcttatc acgccattct tcgctgttaa ttcagtaatt tttcagaatt atgcccggtc
      181 aacgctaaag gcgatgactt cagccagtgt ctccgcgccc agcgccaaca tcaccagacg
      241 atcaacacct aatgccacgc cggaacagtc aggcatacca actttcaagg cttcaatcag
      301 attctggtca atggggtgct gcggcaaacc gcgcgccgcg cgcttacggt tatcttgttc
      361 aaagcgttgt tgctgctcac gggcatccgt taattcatgg aaaccattcg ccagctcaat
      421 acctttataa taaacctcaa agcgttcagc gacccgatga tcttcggtac tgatttgcgc
      481 cagtgatgcc tggctggctg gaaagtggta cacaaaggtc ggtttttctt taccaatatt
      541 tggctctacg ccaaaggtaa acagcaattg tagcaacgtg tcgcggtctt cttcggtatc
      601 agcaacattg ctcaaatcca gtttcgctgc gacttcccgc agttgcgttt tgtcggcaga
      661 gagcgggtca atttccagat aacgcaagaa agcttgttga taagaaaggc tttctgctgc
      721 cgggcagtcc agcacctgtt gtaagagatc gtccacctcg ttcatcaacc ggtacatatc
      781 atagtgcggt cgataccact ccagcatagt gaactcaggg ttgtgataac gccccatctc
      841 ttcattacgg aagctgcggc acagctggaa taccggccca caaccagcaa ccagcaggcg
      901 tttcatatgg tattccgggc tggtcattaa ccagagattc atcccctgcg aatgcccggg
      961 gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg gttaccgtcg cctggctcat
     1021 acaaggcgtc tccacctcca gcactccacg atcggcaaag aaacgacgga tctccgccat
     1081 aatcgccgcg cgttttaata agttaggaat ggatgcgctc ggctgccagg atgccgtttc
     1141 gctcatagtt aaatctccag tttttgacaa gggcacgaag tctactcgca acgcgacggc
     1201 gagacaaatt ttacgcagga atcaaacagc ggtgggcagt gactaaaaaa agcacgatct
     1261 gatggtttag taattaaatt aatcatcttc agtgataatt tagccctctt gcgcactaaa
     1321 aaaatcgatc tcgtcaaatt tcagacttat ccatcagact atactgttgt acctataaag
     1381 gagcagtgga atagcgttcg cagaccgtaa ctttcaggca cttaccctga agtacggggc
     1441 tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct ttcaagccga tcttgccatt
     1501 gtaggcgccg gtggcgcggg attacgtgct gcaattgctg ccgcgcaggc aaatccaaat
     1561 gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta gccataccgt tgctgcagaa
     1621 gggggctccg ccgctgtcgc gcaggatcat gacagcttcg aatatcactt tcacgataca
     1681 gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg attatttcgt ccaccactgc
     1741 ccaaccgaaa tgacccaact ggaactgtgg ggatgcccat ggagccgtcg cccggatggt
     1801 agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc gcacctggtt cgccgccgat
     1861 aagaccggct tccatatgct gcacacgctg ttccagacct ctctgcaatt cccgcagatc
     1921 cagcgttttg acgaacattt cgtgctggat attctggttg atgatggtca tgttcgcggc
     1981 ctggtagcaa taaacatgat ggaaggcacg ttggtgcaga tccgtgctaa cgcggtcgtt
     2041 atggctaccg gcggtgcggg tcgcgtttat cgttacaaca ccaacggcgg catcgttacc
     2101 ggtgacggta tgggtatggc gctaagccac ggcgttccgc tgcgtgacat ggaattcgtt
     2161 cagtatcacc caaccggtct gccaggttcc ggtatcctga tgaccgaagg ctgccgtggt
     2221 gaaggcggta ttctggtcaa caaaaatggc taccgttatc tgcaagatta cggcatgggc
     2281 ccggaaactc cgctgggcga gccgaaaaac aaatatatgg aactgggtcc acgcgacaaa
     2341 gtttctcagg ccttctggca cgaatggcgt aaaggcaaca ccatccccac gccgcgtggt
     2401 gatgtggttt atctcgacct gcgtcacctc ggcgagaaaa aactgcatga acgtctgccg
     2461 ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc cggttaaaga accgattccg
     2521 gtacgtccga ctgcacacta caccatgggc ggtatcgaaa ccgatcagaa ctgtgaaacc
     2581 cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg ttggtctgca cggtgcaaac
     2641 cgtctgggct ccaactccct ggcggaactg gtggtcttcg gccgtctggc cggtgaacaa
     2701 gcgacagagc gtgcagcaac tgccggtaat ggcaacgaag cggcaattga agcgcaggca
     2761 gctggcgttg aacaacgtct gaaagatcta gttaaccagg atggcggcga aaactgggcg
     2821 aagatccgcg acgaaatggg cctggcaatg gaagaaggtt gcggtatcta ccgtacgccg
     2881 gaactgatgc agaaaaccat cgacaagctg gcagagctgc aggaacgctt caagcgcgtg
     2941 cgcatcaccg acacctccag cgtgttcaac accgacctgc tctacaccat tgaactgagc
     3001 cacggtctga acgttgctga atgtatggcg cactccgcaa tggcacgtaa agagtcccgc
     3061 ggcgcacacc agcgtctgga cgaaggttgc accgagcgtg acgacgtcaa ctttctcaaa
     3121 cacaccctcg ccttccgcga tgctgatggc acgactcgcc tggagtacag cgacgtgaag
     3181 attactacgc tgccgccagc taaacgcgtt tacggtggcg aagcggatgc agccgataag
     3241 gcggaagcag ccaataataa ggagaaggcg aatggctgag atgaaaaacc tgaaaattga
     3301 ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat agcgcattct atgaagtgcc
     3361 ttatgacgca actacctcat tactggatgc gctgggctac atcaaagaca acctggcacc
     3421 ggacctgagt taccgctggt cctgccgtat ggcgatttgt ggttcctgcg gcatgatggt
     3481 taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt gattacaccg acggtatgaa
     3541 ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata tgacccactt
     3601 catcgaaagt ctggaagcga tcaaaccgta catcatcggc aactcccgca ccgcggatca
     3661 gggtactaac atccagaccc cggcgcagat ggcaaagtat caccagttct ccggttgcat
     3721 caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag agttcatcgg
     3781 tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc acggtaagaa
     3841 ggagcgtatg gcgcagttga acagccagaa cggtgtatgg agctgtactt tcgtgggcta
     3901 ttgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcagc agggcaaagt
     3961 agaaagttcg aaagactttc ttatcgcgac cctgaaacca cgctaaggag tgcaacatga
     4021 cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa aaattgccgt
     4081 tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg ttcagcattg
     4141 aactgatttt cgggctgttt gccctgaaaa atggcccgga agcctgggcg ggattcgtcg
     4201 actttttaca aaacccggtt atcgtgatca ttaacctgat cactctgacg gcagctctgc
     4261 tgcacaccaa aacctggttt gaactggcac cgaaagcggc caatatcatt gtaaaagacg
     4321 aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg gttgccacca
     4381 tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa tccaaatcca
     4441 aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat gtggagcgca
     4501 attattgcgc cggtgatgat cctgctggtg ggtattctgc tgccactggg gctgtttccg
     4561 ggtgatgcgc tgagctacga gcgcgttctg gcgttcgcgc agagcttcat tggtcgcgta
     4621 ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat gcaccacgcg
     4681 atgcacgatc tgaaaatcca cgtgcctgcg ggcaaatggg ttttctacgg tctggctgct
     4741 atcctgacag ttgtcacgct gattggtgtc gttacaatct aacgcatcgc caatgtaaat
     4801 ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccttatgttc aaaacgacgc
     4861 tctgcgcctt attaattacc gcctcttact ccacatttgc tgcccctcaa caaatcaacg
     4921 atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc ggtatggcgg
     4981 tggcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg gacatcgcca
     5041 aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc aaaacattta
     5101 cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc gatcccgcaa
     5161 caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta ttacatcttg
     5221 cgacctacac cgctggcggc ctgccattgc aggtgccgga tgaagtgaaa tcctcaagcg
     5281 acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca caacgtctgt
     5341 atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct ggtttgagtt
     5401 ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa actcaaccat acgtggatta
     5461 atgtacctcc cccagaagaa aagaattacg cctggggata tcgcgaaggt aaggcagtgc
     5521 atgtttcgcc aggggcgtta gatgctgaag cttatggtgt gaagtcgacc attgaagata
     5581 tggcccgctg ggtgcaaagc aatttacccc cccttgatat caatgagaaa acgcttcaac
     5641 aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat cagggcctgg
     5701 gctgggaaat gctggactgg ccggtcaatc ctgacagcat cattaacggc agtgacaata
     5761 aaattgcact ggcagcacgc ccggtaaaag cgattaagcc cccaactcct gcagtacgcg
     5821 catcatgggt acataaaacg ggggcgaccg gcggatttgg tagctatgtc gcgtttattc
     5881 cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctatcccaat ccagcgagag
     5941 tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa tttcatcggg tccgaatttt
     6001 cggacctttt ctgcgctttt ccttgctgtc atctacactt agaaaaaaac cagtaaggaa
     6061 acatttatgc gcctgctccc tctcgttgcc gcagcgacag ctgcatttct ggtcgttgcc
     6121 tgtagttttc ctacgccgcc gcgtggcgtg accgtagtga ataatttcga cgccaaacgt
     6181 tatctcggta cctggtatga gattgcccgt tttgatcacc gttttgaacg tggactggaa
     6241 aaagtcaccg caacatacag cctgcgtgat gacggcggcc tgaatgtcat taataaaggc
     6301 tataaccctg acagaggaat gtggcagcag agtgaaggga aagcgtactt taccggcgca
     6361 ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg ttataacgtt
     6421 attgcactcg atcgggaata ccgccatgcg ctggtttgcg gcccggaccg cgactacctg
     6481 tggatactct cccgcacgcc aaccatttct gacgaagtga aacaggagat gctggcagtc
     6541 gcgacccggg aagggtttga tgtcagtaag tttatttggg tacagcagcc cggtagttag
     6601 tgagtgctga gtttcagacc aataatcccc aatacgatta acgccagact cgccagacgc
     6661 atcgggttag cggactcacc gagcagcaca atgccggtga tggccgcgcc gactgcgcca
     6721 atacccgtcc acacggcata agccgtccct accggtaacg atttcatcgc ccaggcaagt
     6781 agcgccatac tgactatcat cgccgtcacg gtaataacac tcggcgtcaa acggctaaag
     6841 ccgtgggtat atttcaggcc aacggcccat accacttcca gcagaccagc aataactaag
     6901 ataatccagg acatatcagg ctccggaaat ttggggccgt ccccggtgaa aagaggcgtt
     6961 tgcaggtcgt cctgcaaagc tgatggttga acggatattt tgccaggtgg caggggaatt
     7021 tcaactatta cctttatgac gatcgcagaa aaaacaaacg gcacgacact gctgtcgtac
     7081 cgttattgct tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac cgccatcaga
     7141 aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag
     7201 aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt
     7261 atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg atggaaatct
     7321 gatgattttt cggaagagaa gaaaaattag ctggcagcgc gagagatgga gttaccgaga
     7381 tgtttgatgt cttcgccgaa accgcaagcg gtgttacagc ccgtgagcag cgtgctggca
     7441 agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaataagtg atggtgcagc
     7501 ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta
     7561 tccactttga tgacttcgcc gatttgtacg aacagcggaa ctttaaccac agcgccagta
     7621 gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta
     7681 tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac
     7741 agagttacga tacactctgc ctgatccagc agccatttag cattgtcgcc aattgctttt
     7801 gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg
     7861 ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat
     7921 ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat
     7981 gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct
     8041 aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct
     8101 gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc
     8161 atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg atgaacttct
     8221 gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct
     8281 gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga
     8341 tcctcttttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgt ccggattctc
     8401 caccgaccca ctggaagaac agcacagcgt agtgcctggt ttgttgcata aataccacaa
     8461 ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg
     8521 ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg cacttgagta
     8581 tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgatc cgctgatggc
     8641 gaaagagcac gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg
     8701 tctgcggatt cacagccgtc tgccgattgt gatcccggca cgtatcaccg aggcgctggt
     8761 tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg aatcacatca atcatgccaa
     8821 tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg cgccgtgtcg gtgtcaccct
     8881 gcttaaccag agcgttctgt tacgtggtgt gaacgataac gcacaaacgt tggcaaacct
     8941 gagtaatgcg ttgttcgatg ccggcgtgat gccgtattac ctgcatgtgc tagataaagt
     9001 acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt
     9061 gctgacattg gtgtcgggat atctggtgcc gaaactggcg cgagaaattg gcggcgaacc
     9121 cagcaaaacg ccgctggatc tccagctacg ccagcagtaa ctgatt
//
LOCUS       DAJTUN010000137         9126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTUN010000137 DAJTUN010000000
VERSION     DAJTUN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.137"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..339
                     /locus_tag="OO470_003358"
     CDS             <1..339
                     /locus_tag="OO470_003358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252499.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            527..1642
                     /locus_tag="OO470_003359"
     CDS             527..1642
                     /locus_tag="OO470_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252500.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="OO470_003360"
     CDS             2321..2539
                     /locus_tag="OO470_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6252501.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="OO470_003361"
     CDS             2757..2999
                     /locus_tag="OO470_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252502.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO470_003362"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OO470_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6252503.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO470_003363"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OO470_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6252504.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO470_003364"
     CDS             complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OO470_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6252505.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO470_003365"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OO470_003365"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6252506.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
BASE COUNT         2317 a         2212 c         2302 g         2295 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgat
//
LOCUS       DAJTUN010000138         9073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTUN010000138 DAJTUN010000000
VERSION     DAJTUN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.138"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            397..1092
                     /gene="minC"
                     /locus_tag="OO470_003366"
     CDS             397..1092
                     /gene="minC"
                     /locus_tag="OO470_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6252507.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1116..1928
                     /gene="minD"
                     /locus_tag="OO470_003367"
     CDS             1116..1928
                     /gene="minD"
                     /locus_tag="OO470_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6252508.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1932..2198
                     /gene="minE"
                     /locus_tag="OO470_003368"
     CDS             1932..2198
                     /gene="minE"
                     /locus_tag="OO470_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6252509.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2570..2914)
                     /locus_tag="OO470_003369"
                     /pseudo
     CDS             complement(2570..2914)
                     /locus_tag="OO470_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2949..3068)
                     /locus_tag="OO470_003370"
     CDS             complement(2949..3068)
                     /locus_tag="OO470_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6252510.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3029..3214)
                     /locus_tag="OO470_003371"
     CDS             complement(3029..3214)
                     /locus_tag="OO470_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252511.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3315..3488
                     /locus_tag="OO470_003372"
     CDS             3315..3488
                     /locus_tag="OO470_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252512.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3490..3834
                     /locus_tag="OO470_003373"
     CDS             3490..3834
                     /locus_tag="OO470_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6252513.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3838..4173
                     /locus_tag="OO470_003374"
     CDS             3838..4173
                     /locus_tag="OO470_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6252514.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4230..6551)
                     /locus_tag="OO470_003375"
     CDS             complement(4230..6551)
                     /locus_tag="OO470_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6252515.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6668..6877)
                     /locus_tag="OO470_003376"
     CDS             complement(6668..6877)
                     /locus_tag="OO470_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252516.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7628..>9073)
                     /locus_tag="OO470_003377"
     CDS             complement(7628..>9073)
                     /locus_tag="OO470_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HCR6252517.1"
                     /translation="WYSAKAEYLAGARYAANNINHILEEASQATQTAVNIAGKECDLE
                     EQYQLGTEAALKPHLRTIIILKQGIVWCTSLPGNRVLLSRIPVVPDSNLLLAPAIDTV
                     NRLPILLYQSQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGDVMTLN
                     GHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAAFLLDRYFN
                     KSATPEEILRRAINNGEIVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPL
                     AEKSGLIVPLTQSLMNQVARQMNAIASKLPEGFHIGINFSASHIISPTFVDECLNFRD
                     SFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLH
                     IDYIKIDHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLGYLNQNNIT
                     FQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2536 a         2097 c         1992 g         2448 t
ORIGIN      
        1 tctatgatag agcaattctg tggcggatat taagataacg ggtatctgtt ctttaatgat
       61 gctgcaaaaa atggaaagtt aagaaagcct ggcgtggtga aaaaatacac ttgcctcaat
      121 actggatatt taaacaggtc tggagatgct gtcacgatta gggtgcggaa aaaggaagcg
      181 taaataagaa gaatcaaaag tacgacggca atgggttgat tgacaagggt attttttaag
      241 ctatgaatca gcgccattta tcacagaata gacttttact ctgaataaat gggagggtga
      301 cttgcctcaa tataatccag actataacat gccttatagt cttcggaaca tcatcgcgcg
      361 ctggcgatga ttaatagcta attgagtaag gccaggatgt caaacactcc aatcgagctt
      421 aaaggcagta gcttcacttt atctgtggtt catctgcatg aggcagaacc taaggttatc
      481 catcaggcgc tgaaagacaa aatcgctcag gcccccacat ttttaaaaca tgcccccgtt
      541 gtactcaacg tcagtgcact ggaagacccg gtaaactggt cagcgatgca taaggcggtt
      601 tcggcaaccg gtttgcgggt tattggcgta agtggctgca aagatgcgca acttaaagcc
      661 gaaattgaaa aaatggggct gcctatcctg acggaaggaa aagaaaaagc gccacgtcca
      721 gctcccgcac cgcaggctcc agcgcaaaat acaatgccgg tcacaaaaac gcgtttaata
      781 gataccccgg tgcgttccgg tcagcgtatt tatgctccac aatgtgatct gattgttaca
      841 agccacgtta gcgctggggc cgaattgatt gccgatggga acattcatgt ctatggcatg
      901 atgcgcggtc gtgcgctggc aggggcaagt ggtgaccggg aaacgcaaat attttgtacg
      961 aacctgatgg cggaattggt gtccatcgca ggtgaatact ggctgagtga tcaaatccca
     1021 gcagaatttt atggcaaagc ggcgcgactg cagttagtcg aaaacgcttt gaccgttcaa
     1081 ccgttaaatt gatccctttt taacaaggaa tttctatggc acgcattatt gttgttactt
     1141 cgggtaaagg gggtgttggt aagacaacct ccagcgcggc catcgccact ggtttggccc
     1201 agaagggaaa gaaaactgtc gtgatagatt ttgatatcgg cctgcgtaat ctcgacctga
     1261 ttatgggttg cgaacgccgg gtcgtttacg atttcgtcaa cgtcattcag ggcgatgcaa
     1321 cgctaaatca ggcgttaatt aaagataagc gtactgaaaa tctctatatt cttccggcat
     1381 cgcaaacacg cgataaagat gccctcaccc gtgaaggggt cgccaaagtt cttgatgatc
     1441 tgaaagcgat ggattttgaa tttatcgttt gtgactcccc ggcagggatt gaaaccggtg
     1501 cgttaatggc actctatttt gcagacgaag ccattattac caccaacccg gaagtctcct
     1561 cagtacgcga ctctgaccgt attttaggca ttctggcgtc aaaatcacgc cgcgcagaaa
     1621 atggcgaaga gcctattaaa gagcacctgc tgttaacgcg ctataaccca ggccgcgtaa
     1681 gcagaggtga catgctgagc atggaagatg tgctggagat cctgcgcatc aaactcgtcg
     1741 gcgtgatccc agaggatcaa tcagtattgc gcgcctctaa ccagggtgaa ccggtcattc
     1801 tcgacattaa cgccgatgcg ggtaaagcct acgcagatac cgtagaacgt ctgttgggag
     1861 aagaacgtcc tttccgcttc attgaagaag agaagaaagg cttcctcaaa cgcttgttcg
     1921 gaggataagt tatggcatta ctcgatttct ttctctcgcg gaagaagaac acagccaaca
     1981 ttgcaaaaga acggctgcag attattgttg ctgaacgccg tcgcagcgat gcagaaccgc
     2041 attatctgcc gcagttgcgt aaagatattc ttgaggtcat ttgtaaatac gtacaaattg
     2101 atcctgagat ggtaaccgta cagcttgagc aaaaagatgg cgatatttct attcttgagc
     2161 tgaacgtgac cttaccggaa gcagaagagc tgaaataagc ccgctgtaaa aacgcattta
     2221 tcttcaaggc agagtcattt ctgccttgag tttttcatcc ctctcatcca cgttgtggta
     2281 aagcggcgag tattcttgct gatactcctc attgctattt ccacctcccc ctttacacct
     2341 taaggctgtt cccacgcaca gaacaaaatg tttatgcttg gttgagtaaa taaccatgtt
     2401 attagtgtgt atatactggg agatgataac cccgcaattt cattattcat tgttctccca
     2461 taggatgagg tttcccgccg taactggcgg gctttttttg ccccagaatt ttctctttca
     2521 acatcctgta aacgaaaact gcgccgaagc gcagtttaat cgttgatggt cagaacgacc
     2581 agttcacacc agccaccgcg ttccacgggg actccacacc ggcaccatgg ctatacccca
     2641 ccccgaaatg cccgcttaac gtactgctga atgaggcttt aatacctgcc tggtatattc
     2701 cacgtctgcc cgacaaatca ttgacaaaat taccgtcact attcactttc acccgattat
     2761 catcgacaaa ttctttgcgc acagccgcct tcaggcaggg ttcaatttcc ataccgttcc
     2821 ccagacgcat gttgtaactc agcgttgcac ccagttcacg atatatactg cgggtatcga
     2881 ctgatttcga ttccatgcca ttggataaat gatagtagcg tctaagtgac ctaagctctg
     2941 ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga cagcgcaggt
     3001 gtattgtgtt caccgctcac caaagacatc atgatgaaat gatgatatcc cgcataagaa
     3061 agaggcattt tttacacgca gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca
     3121 gataatactg tcgtacgctt atctttagac aatgaggccg tcggaaaaat aaaatgacta
     3181 atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat aatattgata
     3241 aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt cttgaataaa
     3301 aatattgtca atcaatgagt tattcctcat ttaagattat tctcattcac gtaaaaaaca
     3361 taattcctat catcacagcc acgctcattt tgaattatct gaataatagc gagcgttctt
     3421 tagttaagca gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac ttaataccgg
     3481 acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt gttctgctcc
     3541 acgccagcga tggcggatat gaaccgcacc acgaaaggtg cattgctagg cgcaggcgtt
     3601 ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt tggcgctggt
     3661 gttggtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg tgcaaaagtg
     3721 ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga aggggcgatt
     3781 aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg
     3841 atgatgaaaa aatttgcttt actggcgggc ctgtttgttt tcgcaccaat gacctgggcg
     3901 caggactaca atattaaaaa cggtttgccg tcagaaacct atattacttg cgcagaagcc
     3961 aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt aatgggtaat
     4021 gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag cgccaaagtg
     4081 gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt
     4141 gatgacacca tgcgtgctat cggcaaaaaa taatatttca tatgtaccgc gtcgatgaat
     4201 acgacgcggt tttttagcta acggcgggat cagaacttat atttcaggcc caccatcact
     4261 gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattccccca gacgctggcc
     4321 gctggattca ggttgccatt tacgccaagt ttaatttccc ccaaattact caccccatct
     4381 tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg gatccagttc
     4441 atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc ctgagtacga
     4501 ataaaggtgc ggaagccgag tcgggtctgg atgttgttat ttgcgtcatt ctcaatacgt
     4561 gagccattgg cttccgtgtg cgcattatga tcgacgccca tccagatagc ctgtgcctgt
     4621 ggctggaaga tccacgtata tttcgccgct tccagaccaa aggatttatt cagggcaaaa
     4681 ccgtaacccg cttccagcga gacggttgca cctttcgcgc tataggattc ataggctaat
     4741 tcatcccctt tcacggagtt tttaaaccag ctatattgcg cccaggagtc aatgtatgcg
     4801 ccttttttac tgatgtcatc ggcaaaccaa gtggcataca gccctgcgct atagcctctg
     4861 acactccctt tcgaacgata atccgacacg ctggaatgag ttaagttgta gtcgcgggca
     4921 taaccagcca tcactcccaa acgccaactg tcactatcgg taaaaccacc ggttaacaga
     4981 tcgcccccta actgcgagac gtagcgatgc gaggtcgttc tcaactgtcc gttgctgtca
     5041 cgccaggcat tatgcccgcc aatttgacgt agccaaagtc ggcttgaacg ctcctgttca
     5101 gttacaggat cgatgtagcg cgtttcaccc gcacggtcgt tcagacgcat aacaaaaaga
     5161 gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt cggggcgcag gacgggggta
     5221 ggcgcaacag taggcgctga acttcctccg gagtttgttt caccgccgct cgttgcctgg
     5281 aagttagtca gataccagtt tttattggtc gcttcggtat tacctcgggt taatgaatat
     5341 tcgtaggcac cggcgaaaat cctcgaatcc ttaataaatt ccccatttga ttccccctca
     5401 acgctgataa tctctatccc atttaacgtg taagctccgc tgcctcctgc attggtaact
     5461 ttaactcggg ttgttccaga ggtatttcca ttaataacga ctttatccgt agctgaatta
     5521 tcatcatcca attcagttct gagatgcaat acgccattat taccaacgta attttcagtt
     5581 attgttaatc ttgttggttc gacatcccgt ccatctgccc gggaaatata gaccgtacta
     5641 ttataaaccg tgaggttgct caccgttgag tcagctttaa tatcccaggt actattatct
     5701 gaaagcgaca gataggtatg agtgttatca tctgttgaaa tattagctga acccgttatt
     5761 tttgaattag ttgcatcgac ggtcaaatga cctttaatat tattgataaa aacatctgca
     5821 gccgatgata cctcactatc cgtaatggtc acgttactcg tctcattttt atggtagagg
     5881 atttcatttg ctgaccgtag tgagctacca gataaaatat ctaccgttgc ttcctgacca
     5941 tttatggaag actccagcac aattccagac ccctgatgcg cgactgcaga tgtatttttc
     6001 agcgtcaccg tgccgccatt ataaatttta gcaatcgcgg cgtcaggacc cgttgtataa
     6061 atttctccct ggttaaatga aactgtcccc tggcgggcag cgatggcatg agcattgtcc
     6121 ccatgcgtgg tgatatcaac atcggttaat gtaacgctgg cattggtata atcaagcgtg
     6181 tcaccagcct tgacgacagt gccattagcg tacgcggcat aactttctgt tccgtgggtt
     6241 gtgatctccc cttttgccat atccagtttt gctatggcat tattttcatt acttcctgta
     6301 tcggcagtac tttcttcatc aagagaagat acacacgcac acgcgcttcc gggagatgca
     6361 cacgcaagaa cgccgactgc gtgattaccc gcagtaacaa taaatgtatc atctccgtca
     6421 gaaaaagagg gggtaaccga accgtcagag ttgactctta ttatggtgtc agtattcagg
     6481 tttccactcg tttgacttag caaaccaatt gagtaatccc ccgttgtgac aatcgtgttt
     6541 ttaccctgca aaataacagc accgccaaat tcacttacga ttgcgctagc tgttaatgct
     6601 gggcttagag gactattgtc agaaggatat aaaccgcccg ttgttacaat gacgccattg
     6661 ttaatggtta acttactacc ctctcctgca acggataccc gtaacttcca ttacccgttg
     6721 ttataactag cgcattatca atatctttta cttttccatc ttttattgcc acagcatgtt
     6781 tatattcatc gtgctgaata aactgttcag catatgcatt acccaccgcc agagcaataa
     6841 gtcccaaact aaacccgggg agttttttca gtttcataga ttaactatcc attcattgga
     6901 ttaacaaaca ttcctggtca aataacctat gagctcagat taccaaaacg gagggtaaag
     6961 acaaaaacaa aagcacaaaa aatttaaata gataaacagc gacaatatat aaatgataaa
     7021 taagtcggca ttatcatttt tcaccttatt ttcatgacat tgatcacttt ggggtgacta
     7081 ttttgtttaa caaataactc ttcgacatca aaattattaa tagttgccat ggtaattatt
     7141 tcggtgatat ataaaaacaa ctgtgataac accaaggttg acaactattt gcgcaataat
     7201 tcctttcctt caatgatcca ggcgttcact gccagcaacc cattcagccg catactgaca
     7261 gtgagaaact gaaagattat tgctgatccc tgtggctaac gagtctttct cttaaatcga
     7321 ttaaccagat tgatcgccag ccaaacaagg gcattaagca aatttatcgt cacaagcact
     7381 tctcccagcc cggtactggg ttttgcaata aggatgacca ggagggtgat cagcataaat
     7441 gcaatggaaa agatgatata gagagtgaaa taatccagat tattactatt attcatgcta
     7501 ctaatggcaa tcctgtcaaa actttcaaac aattatagca aacaaaataa tgaacaatag
     7561 taacaagagg caacgagcta attagctgtc tataattaca ccgcactatg gccttactga
     7621 tcaattttca ctcaaccata accttcacct tcggtttaga aaggataatt ttgaccaggt
     7681 cgatgtatgt aacaggttta tagaaataat aaccctgctg aaatgtgata ttattttggt
     7741 tcagatagcc aagttgttct ttcgtttcga caccttcagc gacgatactg atcgaaagtt
     7801 tacgcgccag atccaataca caatccagaa ttcgggttga ttctgggtct gcgtttacgc
     7861 ggccaacgaa actgtgatca attttgatat aatcaatatg caggtcatga agataagaaa
     7921 gccctgagta gccagtaccg aaatcatcca gcgcgatgac aaaaccattt tcatgcagaa
     7981 tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg ctcacgctcg gtgacttcca
     8041 gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa atttaaacac tcgtcgacaa
     8101 acgtcggcga aataatatgc gaagcgctaa aattaattcc aatatgaaaa ccttccggca
     8161 gtttactcgc gatagcgttc atctgtctgg caacctgatt catcaggctt tgtgtaagcg
     8221 gaacgattaa tccggatttt tcagcaagtg gaataaatgc cgcgggtgaa atatatccac
     8281 cgtgaggttg tttccagcgg gctaacacct caactccccg caatgtccct tcccgaccat
     8341 ttaccacagg ttggtaaaaa gggacgatct ccccattatt tattgcccgt cgcaggatct
     8401 cttcaggcgt tgcgctttta ttgaaatatc tatcaagcag gaaggcagcg gcgcaggcga
     8461 ttaacagaat aaatattaaa atcccaaagc ctttatcgat aagtctgtag aagctaaaga
     8521 gtggcggcgg gttaaagata atagtgaaat gatattttgt ggagtgaacc ttctcggtat
     8581 agggataatg tccattaagc gtcatcacat cacccgttgg tccaataatg tcatccgcca
     8641 cgcgtaatac ataccttacc cctttcaagg gtacattaag cgccccgcga atatgttgat
     8701 cacttatcgt aaccaaaatg cgcgtatctg caaattggct ctgatagagc aggataggta
     8761 atctattaac ggtgtcgatt gccggagcca acagtaaatt actgtccggg acaacaggaa
     8821 tacgagacag caggacccga ttcccaggca gggatgtaca ccagactatt ccctgtttga
     8881 gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc agtgccaagc tgatattgct
     8941 cctcgaggtc gcattccttc ccggcaatgt taaccgctgt ttgagtcgcc tgtgacgctt
     9001 cttcaagtat atgattgata ttgttggcgg catatcttgc tcccgccagg tactctgctt
     9061 ttgcggaata cca
//
LOCUS       DAJTUN010000139         8942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTUN010000139 DAJTUN010000000
VERSION     DAJTUN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.139"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO470_003378"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO470_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6252518.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO470_003379"
     CDS             1828..3147
                     /locus_tag="OO470_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6252519.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO470_003380"
     CDS             3445..3486
                     /locus_tag="OO470_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252520.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO470_003381"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO470_003381"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6252521.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO470_003382"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO470_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO470_003383"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO470_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO470_003384"
     CDS             5815..6711
                     /locus_tag="OO470_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6252522.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="OO470_003385"
     CDS             6708..7694
                     /locus_tag="OO470_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6252523.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="OO470_003386"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="OO470_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..>8942)
                     /gene="osmC"
                     /locus_tag="OO470_003387"
     CDS             complement(8669..>8942)
                     /gene="osmC"
                     /locus_tag="OO470_003387"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6252524.1"
                     /translation="AAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITK
                     IALKSEVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2180 a         2188 c         2256 g         2318 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cg
//
LOCUS       DAJTUN010000140         8935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTUN010000140 DAJTUN010000000
VERSION     DAJTUN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.140"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            28..2212
                     /gene="espL4"
                     /locus_tag="OO470_003388"
                     /pseudo
     CDS             28..2212
                     /gene="espL4"
                     /locus_tag="OO470_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2181..3918)
                     /gene="aceK"
                     /locus_tag="OO470_003389"
                     /pseudo
     CDS             complement(2181..3918)
                     /gene="aceK"
                     /locus_tag="OO470_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4102..5405)
                     /gene="aceA"
                     /locus_tag="OO470_003390"
                     /pseudo
     CDS             complement(4102..5405)
                     /gene="aceA"
                     /locus_tag="OO470_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5435..7036)
                     /gene="aceB"
                     /locus_tag="OO470_003391"
                     /pseudo
     CDS             complement(5435..7036)
                     /gene="aceB"
                     /locus_tag="OO470_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7305..8234)
                     /gene="metA"
                     /locus_tag="OO470_003392"
     CDS             complement(7305..8234)
                     /gene="metA"
                     /locus_tag="OO470_003392"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6252525.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8391..8834
                     /locus_tag="OO470_003393"
     CDS             8391..8834
                     /locus_tag="OO470_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6252526.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2338 a         2171 c         2161 g         2265 t
ORIGIN      
        1 gacatcaata aacaatgaga tatttaaatg attactcgta ttcctcgtag ttctttctct
       61 gcaaatatta ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat
      121 aaagaactcg aggataaatt ttctggcaag gagctggcga cgcctctatt aaaaagcttc
      181 tcagagaact gtagacaaaa tgttcgtcat atctttagca acaaggattt tgtcattaaa
      241 ttttccacgt ccgtcttaca agctgataag aaagaaatta cgataattaa taaaaacgaa
      301 aacacgacac tcactcaaac cattgctaca atatttgaag aatacctaat ggaaatttta
      361 cctcaacgct cagacactct tgataaacaa gaattaaacc taaaatcaga tagaaaagaa
      421 aaagaattcc caagaattaa acttaatggt caatgttatt tcccggggcg ccccaaaacc
      481 gtatagtata ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt
      541 acaaacccca tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa
      601 aatgcaaaaa ccagactttg gcgttgattt ctagccgtcc tgaggggcgt tgcgttgctg
      661 cctgcggtga tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca
      721 tcagtgttat ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa
      781 taaagaacac aactgaagga tgtacccatt acgtggtttc ggtttatgat cctaatgtaa
      841 ctaacgataa aataagaatt atgagcgaaa gcaaagagga tattaaacac tattctctga
      901 tggattttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc
      961 aatctgttgc aataattcca gcacttccga aagaacagct attgatgtta aaaggaactg
     1021 tggatgaaat aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc
     1081 agaatcacca actcacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa
     1141 cagaaatgtt gactgcctat aatagtatta acctccccgg tttatatttg gctataaatt
     1201 atggcaatgc ggatatcgtt gagactattt tcaattcatt gtcagaaaca ggatatgaag
     1261 ggttactctc gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa aatggttttt
     1321 ctggattatt tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg
     1381 tcttacctaa actggccaca acacatcatt tagataatga acaggtgtat aaattcctga
     1441 gtgccaaaaa tagaacgtcc agccatgttt tataccatat tatggcgaat ggtgatgccg
     1501 acatgctgaa aatttttttg gtcgcgttac ctttgttaat tcgcacatgt catttgacta
     1561 aagaacaggt actcgatctc ctgaaggcaa aggattttta tggttgccca agactatacc
     1621 tggcgatgca aaatggacat agcgatatcg tgaaagttat tctcgaagca ttgccctgtc
     1681 tggcccagga aattaacatt tcagcttccg atattgtaga tcttctgacc gctaaaagtc
     1741 ttgcgcgcga cacgggtttg tttatggcca tgcagcgcgg acatatgaac gttattaata
     1801 ctatttttaa cgcattaccc actcttttta atacgtttaa attcgataaa aaaatatgaa
     1861 gcccctcctc ctggcaaata attctaatga atatcccggt ttgttttcag cgatacagca
     1921 taaacaacaa aacgttgtag agacggttta tcttgcttta tctgaccatg cacgcctgtt
     1981 tggatttacc gctgaagata ttatggattt ttggcaacac aaagcgccac aaaaatactc
     2041 tgcctttgag ttggcttttg aatttggtca ccgggttatt gctgaattaa tccttaatac
     2101 attaaataag atggctgaaa gctttggctt tacggataac cctcgataca ttgcggagaa
     2161 aaattatatg gaagctttac tcaaaaaagc atctccccat accgtacgct aaatctttgc
     2221 ctgcgccgat acgcataaac atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg
     2281 cgccagtaat cagcgcagaa caggtcggcg tgcatctctt caaacagcgg accaatacgc
     2341 gggtcggcgc atagccagtg gcgaaactct tccgggaaaa catcgcccgg cgagacgctg
     2401 taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaaa
     2461 ttcacttccg tcatgtagca aatttcatcg taatcataaa aaaccacacg cccgtgacgg
     2521 gtgacaccga agtttttaaa cagcatgtcg ccagggaaaa tgttagcagc ggcaagctgg
     2581 cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact gctgaccttc cacttgttcc
     2641 agccagatat tgagcggcac catccgccgc tcgattatag agatggcgaa ttacaatctg
     2701 ctcaccaaga tcggtgattt tttccgctgc ttcctggagc agtaattcca ttaataccgg
     2761 ggaaatatgc cgcttctcca gcacaaagtt ttcaaactcc tgggtgtccg ccattcggcc
     2821 cacacggtcg tgctctttca ccagttgata gcaggcacga acgtgagcgg cagacatctc
     2881 tttctgcggc gcgaacttgt ctttgatgac tttgaatacc cgatcaaagc ccggcagcgt
     2941 aaacaccaac atcaccattc cacgaatacc cggcgcttca atgaactgct cattacagcc
     3001 ctgtagataa acgagatatt cgcggtagct ttcggttttg gcgtgcttct ggcagccgat
     3061 agccatatac aattcagcag tggttttacc tggcagaatt tcccgtagcc actcgaccag
     3121 cgctgcgggc agcggtgcat aaaccataaa ataagaacgc gcaaagccaa aaacaatgct
     3181 cgcttcggcg gtcgtcgtca ggcaggtatc aataaataac tcgccgtcgt ccgtctggtg
     3241 gatcggcagt aagaagggca acgtgccgga aggcgtgatc agtttgccca ccagccaggc
     3301 ggctttattg cggtaaaaca gttcgttcgc cacctgtaaa tgactttccg cgaggttgtc
     3361 tgtccccagc gtttccgtca gatggcgaat gatgtaatga atgtcacggc ttttattctg
     3421 ccagcgcagg cgcagcggca ggtcgctgat aacgcgcatc agtagagatt cccagccgtg
     3481 atcggggtga aagtctttcg ccagcgggcg gggaatggta cgaaagcggc gctctggctg
     3541 agagctaaag ataaaaagcc gctcgggagt aagcgagcgg tggtcaaata accgacagta
     3601 cacggagtta aaaaagctct ccgcaatctc gaagcgcggg taatccggca acagccgcgt
     3661 gtaatgctct ttaacgcgca gtaaaaatgc cgcgtccgta ctttggccgt tagtaatgca
     3721 gcgcagttgc tccacgacca gaccaacgtg atgatcgtaa agatggatac ggtttttcat
     3781 cgcctgctgg acagcatgcc agtcggcctg ttcgaaacgc tgctgcgccc cggaagtcac
     3841 ttcgaggaat cgaccatact gagcgtcgaa gccttgcaaa atggtttgag caatcagtaa
     3901 ttccaggcca cgcggcattt agcgccctca tcaggagcag agaattgccg gatgcggcgt
     3961 aaacgcctta tccagcctac gttcggcaac ggttgtaggc atgataagac gcgcaagcgt
     4021 cgcatcaggc atcgtgcacc gattgccgga tgcggcgtaa atgccttatc cggcctacag
     4081 tcagcaacgt ttgtaggttg cttagaactg cgattcttca gtggagccag tcagcgcggt
     4141 gactgaagac gtgccgccct gaataatagt cgtcacttta tcgaagtaac ctgtacccac
     4201 ttcctgctgg tgagatacga aggtatagcc atctttcgcg gcggcaaatt ccggctgctg
     4261 cactttctca acatagtgct tcataccttc gccctgcgca taagcgtttg ccaggtcgaa
     4321 catgttaaac cacatgctgt ggatacctgc cagggtgatg aactggaact tgtagcccat
     4381 atccgacagc tgctgctgga agctggcaat agttttgtcg tcgaggtttt tctgccagtt
     4441 gaacgacggc gagcagttat aagccagcag tttgcccgga tatttcgcgt ggatagcttg
     4501 tgcaaagcga cgcgccagtt ccagatccgg cgtggaggtt tcacaccaga ccaggtcagc
     4561 atatggcgca tacgccaggc cacggctgat cgcttgctca atgcccgcat gagtacggaa
     4621 gaagccttca ctggtacgct cgccggtaat aaattcgctg tcatacgggt cgcaatctga
     4681 ggtgatcaga tccgccgcat cagcatcggt acgtgcaacc agcagggttg gaacgcccgt
     4741 cacgtcagct gccagacgcg ccgcgaccag tttctgaata gcttcctgag ttggcactaa
     4801 aactttgccg cccatgtgac cgcatttctt cactgacgcc agctgatctt cgaagtgaac
     4861 tgccgcagca ccggcttcaa tcatcgcttt catcagttca aaggcattca ggacaccgcc
     4921 aaaaccggct tccgcatcgg caacgatcgg caggaagtaa tcgacatagc gcggatcgcc
     4981 cggctcaatg cccacggacc attggatctg atcggcacga cggaaggtgt tgttgatccg
     5041 ctccaccaca gctggcaccg agtttgccgg atagagcgac tgatccggat acatgctggc
     5101 cgccaggtta gcgtccgccg ctacctgcca tcccgacaga tagactgctt caatacccgc
     5161 tttcgcctgt tgcagcgcct gaccgccagt cagtgcaccg aggctgttga tgtagctttt
     5221 ttcgactcac cgtgcagcag acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat
     5281 tcaggattga ctgaaccgcg taatttcacc acatcttccg cactgtatgg gcgagtaatg
     5341 ccttcccaac gcggttgagt ccactctttc tgtaattctt caatttgttg tgtacgggtt
     5401 ttcatgtgca gatgctccat agttatgtgg tggtttacgc taacaggcgg tagcctggca
     5461 gggtcaggaa atcaattaac tcatcggaag tggtgatctg ttccatcaag cgtgcggcat
     5521 cgtcaaaacg gccctgggag aaacgttctt cgcccagttc gctggcaatg actttcatct
     5581 cttcgcccag catctggcgg aacaaggctt tggtcaccgg tttgccattg ctcaacgttt
     5641 tttgatgatg gatccactgc cagatcgagg tacgggaaat ttcagccgtc gccgcatctt
     5701 ccatcaggcc ataaatcggc acgcagccgt tgccggagat ccacgcttcg atgtactgca
     5761 cagccacgcg aatgttagcg cgcatacctt cttcggtgcg ttcaccatca caaggtgcca
     5821 gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac
     5881 gggagccgag aatgtcgttg aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga
     5941 tccatgtgcc atcgtgaccg ttattggctt ccagcgcttt atccgctttt actttgttga
     6001 gcacctggtt attacgctct tcatctttgc tcggaataaa cgcggccatg ccgcccatcg
     6061 caaaagcacc gcgtttatgg caggttttaa tcagcaggcg tgagtaagca ttcaggaatg
     6121 gtttatccat cgtcacggcc tgtctgtctg gcaggacacg atcgggatag tttttcaacg
     6181 ttttgatata gctgaagatg taatcccagc gaccgcagtt cagaccaaca atatggtcgc
     6241 gcagcgcgtg aaggatttcg tccatctgga acacggcggg cagcgtttca atcagcaacg
     6301 tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc tgcatagctg aagacttcgc
     6361 tccaccaggc cgcttcctgc taggactggg ttttcggcag atagaaatag ggaccgctgc
     6421 cctttgccaa cagtgcctga tagttgtgga agaaatagag cgcaaaatca aacaggctac
     6481 cggggattgc ctcaccacgc caggtgacat gtttttccgg caagtgcaga ccgcgtaccc
     6541 gacaaatcaa aaccgctgga ttgggcttga gctgataaat tttgcctgct tcattggtat
     6601 agctgatggt accgttaacc gcatcgcgca ggttaatttg cccgtcgatc actttgttcc
     6661 agtctggtgc cagtgaatct tcgaaatcgg ccataaagac tttcacattg gcgttcagtg
     6721 cgttgatcac catcttgcgc tctaccgggc cagttatctc tacgcggcgg tcttctaagt
     6781 ccgcaggaat cccgcgaatt ttccaatcag catcgcgaat ggaagccgtt tccgaaataa
     6841 aatcaggcaa cgttccgtta tcaatatctt gctgctgctg aatgcgcgct gccagaagtt
     6901 tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag aaattctacc gcttcggcag
     6961 taagaatttg cttctcctgc tcgccatacg gccttgtgaa agccagttca tcggttgttg
     7021 ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa
     7081 cgaaggaacg ttgtgcgctt ttcgttcagc acaactaaga ctactcattt aaaatgcaaa
     7141 atcaaaaaca atttccattt taattaaaaa tacttgataa cttattgata acaatatgaa
     7201 taaaatttat tcacttgctg aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct
     7261 gaggtaaggt gctgaatcgc ttaacgatcg actatcacag aagattaatc cagcgttgga
     7321 ttcatgtgcc gtagatcgta tggcgtgatc tgatagacgt aatagttgag ccagttggta
     7381 aacagcaaat taccgtgact acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc
     7441 gggaaatagt tatacggtac atccgggtct agtccggctt ccacatcgcg taaatattcc
     7501 tgcgccagcg tttgcgcatc atattcggga tggcccgtca caaaggcaat gcgcttatct
     7561 ttgctggcaa acagatatgc atccccttct tccgtctctg ccagaatttc cagatcggtg
     7621 taatcacgaa tcaacgctgc cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa
     7681 tcatcaaagc cacgcgtcag aagcgcatga ggatggagaa tatgatgctc gtaaacgcca
     7741 gagagtttgt cggtgcgagt ttgcttagga atgccgtaga ggatattgag cgcggcctgt
     7801 accgcccagc agacaaacag cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt
     7861 ttgatctgcg gccagtaagc gacatcatta aactccacca ggcccagcgg cgcaccggtt
     7921 acaatcagac cgtcaaagtt ctgttcctga atatcttcaa agttacagta gaagttgttc
     7981 agatgctctg cgggcgtgtt gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg
     8041 acctgcaaag gtgagtttga aagcaggcgc agaaactgat tttcagtttc aatcttcttc
     8101 ggcatcaggt taaggatcag aaccttaagt ggacgaattt cctgaccaga cgcacgagaa
     8161 gttgtcatca caaagacgtt ttcttcacgc aagaaattga cggcgggtag ctcgtccggc
     8221 acacgaatcg gcataacctg attacctcac tacatacgct tatacgttta gacatccaga
     8281 tagctgaaga taaccagaaa atttgccaat gtcgagcctg catgttgaag gtgagaaagt
     8341 ttcaaggaaa aggctacgtt agaatataag aatgacgaaa aggagagaaa atggttatta
     8401 gtattcgccg ctcacggcat gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg
     8461 tcgatgccac ccacgatttt ttatcagcag agtatcgggc cgagctggaa gagctggtgc
     8521 gttcctttct gccggaagca ccgttgtggg tcgcggttaa tgaacgggag cagccggttg
     8581 ggtttatgtt gttaagtggg cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg
     8641 gctgcggcgt gggtcggatg ctggtggagc atgcgctctc gatggcaccg gaactgacaa
     8701 ccaacgttaa tgagcaaaat gagcaggcgg ttgggttcta taagaaggtg ggttttaagg
     8761 ttacgggacg ctctgaggtg gacgatttgg ggaaaccgta tccgttgctg aatctggcgt
     8821 atgtggggga ataagccgcc cctgccgtgc ctgcccaagc tggagaagac gcgccagcgt
     8881 cgcacccggc atttttttca ccaacgcaaa aaggccatcc tgacggatag ccttt
//
LOCUS       DAJTUN010000141         8929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTUN010000141 DAJTUN010000000
VERSION     DAJTUN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.141"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO470_003394"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO470_003394"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6252527.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO470_003395"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO470_003395"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6252528.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO470_003396"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO470_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6252529.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO470_003397"
     CDS             6038..6658
                     /locus_tag="OO470_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6252530.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO470_003398"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO470_003398"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6252531.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8926)
                     /gene="bacA"
                     /locus_tag="OO470_003399"
     CDS             complement(8123..8926)
                     /gene="bacA"
                     /locus_tag="OO470_003399"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6252532.1"
                     /translation="MLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDTAKTFEV
                     VIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPAVVLGLL
                     FHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMIGCFQCL
                     ALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGFLTTGDI
                     PMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
BASE COUNT         2080 a         2619 c         2251 g         1979 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccactg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccattgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaagg
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcga
//
LOCUS       DAJTUN010000142         8868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTUN010000142 DAJTUN010000000
VERSION     DAJTUN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.142"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..579
                     /locus_tag="OO470_003400"
                     /pseudo
     CDS             1..579
                     /locus_tag="OO470_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(583..1973)
                     /gene="tcyP"
                     /locus_tag="OO470_003401"
                     /pseudo
     CDS             complement(583..1973)
                     /gene="tcyP"
                     /locus_tag="OO470_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(2106..2696)
                     /locus_tag="OO470_003402"
     CDS             complement(2106..2696)
                     /locus_tag="OO470_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6252533.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2859..3527)
                     /gene="hxpB"
                     /locus_tag="OO470_003403"
     CDS             complement(2859..3527)
                     /gene="hxpB"
                     /locus_tag="OO470_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6252534.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3674..4210
                     /locus_tag="OO470_003404"
     CDS             3674..4210
                     /locus_tag="OO470_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6252535.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4251..5111)
                     /locus_tag="OO470_003405"
     CDS             complement(4251..5111)
                     /locus_tag="OO470_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6252536.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5217..5507)
                     /gene="ghoS"
                     /locus_tag="OO470_003406"
     CDS             complement(5217..5507)
                     /gene="ghoS"
                     /locus_tag="OO470_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6252537.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5608..6537)
                     /gene="pfkB"
                     /locus_tag="OO470_003407"
     CDS             complement(5608..6537)
                     /gene="pfkB"
                     /locus_tag="OO470_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6252538.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6824..7582
                     /locus_tag="OO470_003408"
     CDS             6824..7582
                     /locus_tag="OO470_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6252539.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7635..7742)
                     /locus_tag="OO470_003409"
     CDS             complement(7635..7742)
                     /locus_tag="OO470_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252540.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7914..8868)
                     /gene="espL1"
                     /locus_tag="OO470_003410"
                     /pseudo
     CDS             complement(7914..8868)
                     /gene="espL1"
                     /locus_tag="OO470_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT         2347 a         2076 c         2102 g         2343 t
ORIGIN      
        1 ttagttttga tacgtatctg caggacaatg ttaaatatag agtgataatt aataatcttt
       61 atggcagtga gtttcggata agagatatta atgagaattt tgttgggatg gattttacct
      121 gtgaattccg acccgaaaaa atgacgaatt tgatcaatat aaaaaatagg ttggttattc
      181 attatttaag gactatatac tatgaaaaaa attatataca tcctgccggg agcatttttg
      241 ctgcgattca aaaaaaatga gagtctccta aagtttcctt cgataatatc aatgcttaat
      301 atcaatcttc tttttaatcc actaaatctt ccgggaatgg gaagtggtgt gcttgaagat
      361 attatgagta ttccagatag ttcacttaga aaacgtctcg ggtatgaggt tttaagcttc
      421 tcactacagg cacattcatt gagccaggaa tgtattgata agttggacat ttttttcgct
      481 gacgatctgt ttaaatatga gtcagtatgc atcgccgcga tggaacattt aaaaagtaaa
      541 gcaacggccc ctatacaaaa cggaccgttg ccagcataag aattaacggt gtgccagttc
      601 ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc ttcagccact ggctggtcag
      661 cgtgccagct gtcatcgagc cactaacgtt tagcgccgta cggcccatgt cgataagcgg
      721 ttcaacggag attaacagcg ccaccagggt tactggcagg cccatcgcag gcagcacaat
      781 cagtgcggcg aaagttgcac caccaccgac accggcaacg cctgcggaac taacggtaac
      841 aataccgacc agcgtcgcaa tccacatcgg gtccagcggg ttaatgccaa ccgtaggcgc
      901 aaccatcacc gcagcattgc cggatacaaa ccggcgcagc cgttctgacc aatggttgca
      961 ccgaaagagg cggcgaaact ggcgatggat tcaggaacgc ccagacgacg cgtttgtgct
     1021 tccacattca gtgggataga cgcagcactg gaacggctgg taaaggcaaa cgtcagcaca
     1081 ggccatacct tacggaagta cttcagcgga ctcacgccat taatgcccag cagaatgcca
     1141 tgcacagcaa acataatcag cagaccgagg taggacgcga caacgaaact tcccagtttg
     1201 atgatgtctt gcaagttaga acctgcaacc actttggtca ttagagccag aacgccgtaa
     1261 ggggtcaact gcatgacaag gcgaaccagt ttcatcaccc agctttgtag ggtatcgata
     1321 gcggttaaga cgcgttcccc tttcggcgca tcatccttca gcagtttcaa tgcagctacg
     1381 ccgaggaatg cggcaaaaat taccacgctg atgattgacg tcggattggc tccggtaaga
     1441 tcggcaaacg ggtttttcgg gataaaggac aagaccagct gcggaacgct ctgatcagag
     1501 actttaccaa catagttact ttcaatggcg ttcagacgtg cagtttctgc accaccctga
     1561 accagacctt cagccgtcaa accaaacagg ttggtgacca gcacaccgac cagcgccgca
     1621 atcagcgtgg taaacaaaag cgtaccgatg gtcagaaaac tgattttgcc taactgagat
     1681 gcgttatgca gacgggcaac cgcgctcaga atagaggcga agactaacgg cataacgatc
     1741 atttgcagca gttgaacata gccgttacca acgatgttaa accactgtac agaatcttta
     1801 agtacctggc tgtcagaacc ataaatggta tgcagggcaa ggccaaaaac cacacccatc
     1861 accagaccca ccagcacttt tttcgccaga ctccactgtt tatggcgggt ctgagccagc
     1921 gcaaacagca gtacaacgaa caccacgatg ttcgcaatta atggaaagtt catccccgtt
     1981 ctcctgatct tattatttct atcactgcgt agcagtcata gtgttggcgc aagattaaca
     2041 gaactgtgag ctggctctta tattcaaatg gaattgctta ttccagaaag gtgatttttg
     2101 cctttttagt attcaacctg atgcttaata agccgatgaa actggatctg caaggtattg
     2161 atcatttgcg atgaccaacg aacagcgctg ttctcgggta atgaatgggg catccatacc
     2221 gaccagacaa ataatgccat gcagataaaa cgttccagtt ggttgccctt ttggggaacc
     2281 aggataggca agcggaaacg ccagcggcat ggccagagca ggggaacacc ggcgggtgtc
     2341 agcatatcgg caagtatgtg gctcaaataa cccagcacca ttccttgtag cgcatcagcc
     2401 ggaatgaacc agccttccgg aaccttaagg taaaaggttg ccagcagcgc aaataccgcc
     2461 agcagactgt gggtaaaacc acgatgccca aaagcgcggg cgatcggttt tgatatccat
     2521 tttaatcgct gcccaagaaa cgactttggg tgatcgatgt ccggtaacaa acacgtcagg
     2581 attgcggaag ggacaatatg ccaccagtca ccctgtgcca gcacgggcgt cagctcggca
     2641 tttttggcaa ataccgcaca agcaatagaa aagagaaggt gaccttccgc cgtcatgata
     2701 aaactcacta aactgtcgat acgtacagta taggattttt atacagtgca cggaagtggt
     2761 gacggtgtaa ctctttctct taaattgacg attaatccgt cagttgaatg caaattcaga
     2821 taaagaacca ggggcagcgt gatcactgcc cctggtcatt aaccgagaag gtcttttgcg
     2881 gtgagttctg tcagcgatga gagtttgacg tttgctaata caaaacgtgg atcattttgc
     2941 gcttctggcg caggaacgac gatggaacgc atgcgggctg ctttagaagc gatcatgcca
     3001 tttaccgagt cttccagcgc cacgcaggtc agtgggtcaa cgcccagttt tgctgcgcag
     3061 tcgagatata cttgcggatg cggcttgctg taaggcagtt tttcagccga ggcgagggca
     3121 tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt ccagcatatg tagtggtgac
     3181 gcggaggcca gtcccaccaa taaaccttgt tctttgcata acgcaacagc ttcgcgcacg
     3241 cctggtaata atggacgtgt ctcttcaacc agtgaaatgg cacgggcaat aacccgttct
     3301 actacttcct gacggcttgg cccattccac ggttgccggg cgtaccaaag atcgaccacc
     3361 atatcgatgc gtaaacctaa ggtgtccggc agctcgttac gacgggagat atctaccccc
     3421 aggcttgcca tcacatccag ttcggctcga tcccataaag gttctgagtc gataagtaat
     3481 ccatccatat caaaaattgc agcaagaatc tgacgcgggg ttgacattac aacctctcct
     3541 ttagagcatt atgtgtaagg tattaagccg tgcttatgat taagcgccta tcatatcgcc
     3601 tttaaagaat gggcgaaaac agatatctac aggtaaacta agtgaaacga cgcgtcttaa
     3661 tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt gctgttttga aaaggatttt
     3721 gggttgggtg atttttatac ctgcattgat ctctacattg atttctttgc tgaaatttat
     3781 gaatacccgg caggaaaacc aggaaggcat taatgcggtc atgctcgact ttgctcatgt
     3841 catgatcgat atgatgcagg cgaatacgcc ttttttgaat ctgttctggt ataactcccc
     3901 gacccctaat tttaatggtg gtgtgaacgt tatgttctgg gtgattttca tcctgatttt
     3961 tgtcggactg gcgttgcagg attccggtgc ccggatgagt cgccaggcgc gcttcttgcg
     4021 ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa ggggaagagg ggctaacgcg
     4081 tgaacaaatt gagtcccgta ttgttgttcc gcaccatact attttcctgc agtttttctc
     4141 actatatatc ctgccggtta tttgtattgc tgcaggttat gtgttctttt ctctgcttgg
     4201 gtttatttaa tcaaaaggcc gcacggagcg gccattatcc tcaacccata tcatgctgct
     4261 aataatctat ccaatgactg ctgagcaata accaaatgct gaccgccaaa taatcttgca
     4321 cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt caaggaaatc ggcaggtagc
     4381 ggggatactg actgatagcc gtcatagatt tgtggcggct gttcagtatg cagcggtaac
     4441 atcgctaggt cgcactctcg gtcaccccag tcgcaggcag ggtcgaaaat gtacgggcca
     4501 tccggaccca gcgcacagtt gccggaccat aaatcgccgt gcaacagaga aggctgcggt
     4561 tgatgcgagg ccagacgctg ctgaatatgc tcgacgatgg cgtcgatatt gccgaaagcg
     4621 atccctttct ctgctgccag ttccaactgc cagccaatcc gttgttcagc aaaaaacgtt
     4681 gaccagcgac gttgccaggt gttgggctgt ggagttgtgg agagcgagtt atcaaaatcg
     4741 aggccaaatt gtggttggtc actccattga tgtaaacgcg caatttgctg accaagaata
     4801 aatgcgctat gcgcatccag cggacgaggt gggagataat ccatcaccag aaaactgtag
     4861 tcacggtcag cgcctactgc ccaaacctta ggcacggtga cggttttact acgcgacagt
     4921 aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca gttccctttc atcacatttg
     4981 acgaaaaagt catgtcctgc atagcgcaaa tgccatgcgg catggacttc tccgccaggc
     5041 agttcattac gcagttcgat ttcgccttca cctaactgct cgctcaaaag acgactgatt
     5101 gcctgccaca tgatgtctct cccatgttgt ctgccagatc ataaggttag cgcaagaatg
     5161 cggtgtaaaa acacgagcta acgcacacca cagactgatt attgcccaaa aaccatttat
     5221 tttctgttgc tattccattc ctcccaggtc gtgatggtga tttcaggatc tttgccggtt
     5281 gcactttggg taagccccga aaccagttct ctaatttctt cttcactttg ggtactgata
     5341 agtccaaaag tgttagtccc cagctcatgg atatttccct catcatccgt catggtgagc
     5401 aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa tttcagtcaa tgtatcctca
     5461 tgcaacatga cggttatgac ataacggaca agatcgccac tggccatagt tcaccccttt
     5521 gttatcacat gacttttgag catagtcgga gaaacgcgtt gccgacaggt tggtggtgat
     5581 tcccccaatg ctgggggaat gtttttgtta gcgggaaagg taagcgtaaa ttttttgcgt
     5641 atcgtcatgg gagcacagac gtgttccctg attgagtgtg gctgcactcc ccgcagctac
     5701 gccaaaacgg accatctctt caagagaggc attttctgcc agtttcagtg tcatcgcgcc
     5761 gaccatgctg tcaccagcgc caacggtact ctggcttttc accggtggtg gcaccacctg
     5821 aatgcagttt tcactatcga cacccagcgc tccttgtgga ccaagggaaa cgacaacccg
     5881 tttggccttg ccgctattaa cgatttcctg cgcggctttg cggacatcgt ctggctgggt
     5941 gagttcgcga ttcaccagcg cactgagttc tttttggtta ggcttaacca actcgatgtt
     6001 accaatcgcc agtgctgcac ttaacgcttc gccagaactg tcgatgatgc agcggatccc
     6061 ttggttttgc gcagcggaaa tcagttgggt taatttttcc agcttcacac ctggcggcag
     6121 gcttccgctt atgaccagga tggccccgga ttcaatttcc agaacttgct cttcaagctg
     6181 gcgaaactca tcttcattta atgccgcgcc tggcataaca aaacgatact gttcaccgct
     6241 tgcttccaca tgtacgtgta aattctgccg ggtccagtct ttggcttcta cagtagcgac
     6301 ggggacattt tcatccgcca acagtgaaac caggtgttcg ccggtcgcgc cacccgccgg
     6361 gaagatcgct gtggcactgc ctccaagatg ggcaatggcg cgggcgacgt tgatgccgcc
     6421 gcccccgggt tcgaacaccg gtgcggtaca gcgcagtttt ccttcgggat aaatttgcgg
     6481 ggtaattgtt gcgctatcga gagagggcgc aagtgtcaac gtatagatac gtaccatcat
     6541 ttcctcctat aggctgattt cagtctggca ccgaatcagc ggaaagtgaa gaaattaaca
     6601 atatgattta ttagagcttt atttaaaatt ttgcagataa atatatataa ataaaaatct
     6661 gttttcgcca catgcttatt caaaaaatga aataagaata cattgctaag ttattcgcgc
     6721 ctttttataa ttcgttacct ttccagggac gctaatcttt gatccttaag aaagtttccg
     6781 ggaccgcgat ggtctctttt tttgttgtac ggactcttga taaatgaagc ttttgaagac
     6841 agttcctgcc atcgttatgt tggcgggggg catgtttgcg tcactgaatg cagctgccga
     6901 tgattccgtt tttactgtca tggacgatcc tgccagcgct aaaaaacctt tcgaaggtaa
     6961 tctgaacgca ggatatcttg cacaatcagg caatacgaaa agctcctcac ttacggctga
     7021 caccaccatg acctggtatg gacaaaccac tgcctggtcg ctgtggggga acgccagtaa
     7081 tacttcttct aatgatgagc gttcttcaga aaaatatgcg gcgggcgggc gtagccgctt
     7141 taatttaact gattatgact atttatttgg tcaggcaagc tggctgacag accgttataa
     7201 cggctatcga gagcgcgatg tgttgaccgc gggttatggt cgtcagtttt tgaatggccc
     7261 ggtacacagt ttccgctttg aattcggtcc aggtgtgcgt tacgacaaat ataccgacaa
     7321 tgccagtgaa acccagccgc tgggctatgc ctccggggct tatgcatggc agttgactga
     7381 caacgctaaa tttacgcagg gtgtttcggt ctttggcgcg gaagacacaa cgctgaactc
     7441 cgaaagcgca ttaaatgttg ctattaatga acattttgga ctgaaggtgg cttacaacgt
     7501 cacatggaac tctgaacctc cagagtctgc accggaacat accgaccgtc ggacaacctt
     7561 atcgctgggc tacagcatgt aaaattatct gccggaccgt gttatgcggt ctggctgtct
     7621 tcgtttgtca ctgctcagcc ccaaatcatt gctgcccaac gtagcaacaa catagcgcca
     7681 cagattgcca gtagcaccac caccatcttc cagtgttttt tatcaaagcg tttagtcggc
     7741 ataccagtgt cctgcatatc tcttgtgcgt gataacggag tgtaacaaaa gggacaggca
     7801 tcaagggatt ttttgttatt acagccgtcg ggtggcgttg tgcacatggt cactgtttct
     7861 gcgttttatg aaaaaaataa aattatgtat cgatatatat cgtaaatagc tggttataaa
     7921 tacgtcttta tttcatcagc actaaaactc aggctttggc tgatgacatt tatagcagca
     7981 taatatgttt ctacagtttt ataatgctta tgatgaatgg ctatatgaac agctgacatg
     8041 ttgtcatgat ttttagcggc caatagtgta aataactgat gttgactaaa agaatgtttt
     8101 tttgcaaaag cacccagcgt cgatatataa gataacacga cgtcttcatt acccttgctc
     8161 atggcgatgt ataatgcagg ggttccctgt gctgtagctc cttttaataa atccatgatg
     8221 ttagctttgc tcaacttgta tttaaaggcg atgccattaa ttttagagag gaaccgtgtg
     8281 acacacaaag ggtgattatt ttccattgcg gcgtataatc ctggctcacc atcatgtatt
     8341 ttcatggcta aaatactcat caccagtttt ttgtttaaaa gctcttagtg ctattggagt
     8401 ggttaatgta ttcagaaaga tatcgattat ttcatcgaat ccatacaaca tggagatata
     8461 aagtccaggt gtttcgttag cacttttagt ttgcagtaat ttaaccagat tatcttcatg
     8521 aatgatgtga ttatctacca gtgactgaat ttcttgcata aatattttca cgttgtcagt
     8581 atgtccgttc atcatagcca aaaataaagc aggtgtacca acgctattct gcgcagttat
     8641 tatttcaatg atttcttttt cagggagtat gggcaactgt ttgaataatg taataagctg
     8701 ttgagtctgg cctgtagcca ttattaacaa cattgccgat ggatgtatag gggcaaatgg
     8761 cattcgtgat gcaatacccg cgaaaatagc ccctggaact cccacaggta ataggggcag
     8821 aatagcaatt gcacagttgc gaacatacct atcagcccag gtctgagc
//
LOCUS       DAJTUN010000143         8757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTUN010000143 DAJTUN010000000
VERSION     DAJTUN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.143"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..737
                     /locus_tag="OO470_003411"
     CDS             <1..737
                     /locus_tag="OO470_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6252541.1"
                     /translation="SDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAE
                     QIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAK
                     GSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLT
                     FKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVIL
                     VPGAQSAEAAQKAVSGLVNAMPGGTI"
     gene            complement(968..1378)
                     /gene="rnk"
                     /locus_tag="OO470_003412"
     CDS             complement(968..1378)
                     /gene="rnk"
                     /locus_tag="OO470_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6252542.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(1607..2413)
                     /gene="rna"
                     /locus_tag="OO470_003413"
     CDS             complement(1607..2413)
                     /gene="rna"
                     /locus_tag="OO470_003413"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6252543.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(2527..3989)
                     /gene="citT"
                     /locus_tag="OO470_003414"
                     /pseudo
     CDS             complement(2527..3989)
                     /gene="citT"
                     /locus_tag="OO470_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            complement(4040..4918)
                     /gene="citG"
                     /locus_tag="OO470_003415"
     CDS             complement(4040..4918)
                     /gene="citG"
                     /locus_tag="OO470_003415"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6252544.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(4893..5444)
                     /gene="citX"
                     /locus_tag="OO470_003416"
     CDS             complement(4893..5444)
                     /gene="citX"
                     /locus_tag="OO470_003416"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6252545.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(5448..6980)
                     /gene="citF"
                     /locus_tag="OO470_003417"
     CDS             complement(5448..6980)
                     /gene="citF"
                     /locus_tag="OO470_003417"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6252546.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(6991..7899)
                     /gene="citE"
                     /locus_tag="OO470_003418"
     CDS             complement(6991..7899)
                     /gene="citE"
                     /locus_tag="OO470_003418"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6252547.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(7896..8192)
                     /gene="citD"
                     /locus_tag="OO470_003419"
     CDS             complement(7896..8192)
                     /gene="citD"
                     /locus_tag="OO470_003419"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6252548.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            complement(8207..>8757)
                     /gene="citC"
                     /locus_tag="OO470_003420"
     CDS             complement(8207..>8757)
                     /gene="citC"
                     /locus_tag="OO470_003420"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6252549.1"
                     /translation="QCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         2109 a         2265 c         2409 g         1974 t
ORIGIN      
        1 tttctgatat tctgccaacg gcatggcagg cagcaaaaaa tgtgcagatc caacaaggtt
       61 caagcgttgc agtctatggt gctggtcctg tgggattgtt gacaatcgcc tgtgcacggt
      121 tgctcggtgc ggaacagatt tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg
      181 ctgaccgcta cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa
      241 ttattgaaca aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg
      301 aagcgaaagg cagcaccacg gaaacggtgc tgactaacct gaaactggag ggcagcagcg
      361 gtaaagcgtt gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg
      421 gcgtctacgc tggatttatt cacggtttcc tgtttggcga cgcctttgat aaagggttga
      481 cgtttaaaat gggacagacc cacgttcacg catggctggg agaattatta ccgttaattg
      541 agaaaggatt actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg
      601 cccggggata tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac
      661 ccggtgcaca aagcgcagag gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc
      721 cggggggaac aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata
      781 atcgccgcca taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc
      841 acaaacgaaa cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct
      901 gattttgcgt aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca
      961 gatgggatta aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt
     1021 caaggtgggt tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc
     1081 ccagcagtgc ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt
     1141 ttgccggata caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa
     1201 cccggctgtt cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc
     1261 gatccaactc tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca
     1321 gcagaatatc gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt
     1381 ttactccatg tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc
     1441 accgtcaggc ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa
     1501 gtgatttgcg tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc
     1561 ggcatggtta tggaagacga attagcgtgt tttgtaaatc agaggattaa taacccgctt
     1621 tatcaatcac aaaggttttg ccacagttac ctgggtgagg ttgtggcaag aatgagtttg
     1681 cagaaagcgg agcgttgatg gcgtcagctt tgatcgagat ctgaatttca gtcaaatacg
     1741 cagggttacc ctggcacgtt agcttaactg ctttcacgtt ctcttttccc cagcttttgg
     1801 caaaggcggc gtcaaagtca cggcggctca ctgttttacc gtaattatcc gcaaggaatt
     1861 ttccggcttc gctctcttta atttcttgat tcaggcgtac catcgtaccg aagtatgcgt
     1921 ccggatcaaa accaaagcag gcaccgtgtt tggcatattc gtagcgttcc aggcaggaac
     1981 gtccgccagc tcctggcatg acttcactta gtttagcggc cgtttccagt gataacccgg
     2041 tttccggcga tgaacacatt cggctggcgc gtgcttctgg tagattcggg attgggcgag
     2101 tagcgcaacc gaagcgcatc cagcggcgtt catcaacacc acgggcagca accgatttag
     2161 gcaatcctgg ccacagacca tgtacggtca gaaaatcagt tttgttggtc gtttcggttt
     2221 gcaggcgaca ttcatctcgt tcgttacgat ttcgatcgtg ttgactctgg caaaatccgg
     2281 tttgccagga gagggccagg acatagcgat caaaatcgcc atactgtttt gcctgcaacg
     2341 ctacggcgtt ggcagaagat aagggaagca gagaaaccgc gagcaacgcg gcgttacggc
     2401 agaatgcttt cataatgggt gtggaactca tacatacact gaatactatc tattaaatca
     2461 taaaaaagcc cgccaggtgg gcttacctgg cgggcgtgat gatttattca gcgtttggcg
     2521 aacgtattag ttccacatgg cgagaatcgg ccagccaacc aacagcaaca tagagatgta
     2581 aatcaccccg aagattgcgc caagacgcca gtaatctttt gatttcacat agccacagcc
     2641 gtaaataatc accccaggac cggttgcata cggcgtcaga cagcccatga taccgataga
     2701 cagcaccagc aggatacaca gttgttccat tggtacgccc ggaatacctt taccgacggc
     2761 cagaataacc ggcagcatgg ttgcggtgtg cgcagacagg ctggcaaaca ggtagtgtgc
     2821 aaagtagaac accagaacca gtacaatcac cgttgcgttt ggtgagaatc cttccaggtg
     2881 cgtactcatg gtaccggcga accagtcaat aaaaccagaa cgagtcaggc cgttagccat
     2941 cacaaccaga gttgccaggt tgaccagcgt gttccatgcg ctgttatagc gggtaatgtc
     3001 tttccacggc acaacgtgca gagccagcat tagcgaaact gccagcagac caaccgcagt
     3061 agcattaatg acttcactgc caaataccca caaacctaag ctgagcaata caaggccaat
     3121 cagcgtccac tctctgcgtg tcagcgcacc catggttttt agttcatcac ccgcccaggt
     3181 tgccacttct tcactgtgtg tgatttccgg tttgtacagc acgtaggaaa gccacggcgc
     3241 aatgataagc aagataaccc caaccggcag gaagcagagg aaccactgca accagctaat
     3301 ctggataccg gcaattttgc tgacgaactc cagacccagc acgtttggtc cgcaccggtg
     3361 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     3421 atacgacgcg cggacggatc gttcgggaat gatttaaaca acggcggcag gtttttaatg
     3481 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     3541 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgtccat gaatttcacc
     3601 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     3661 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccaggcccca tttcagcgcc
     3721 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     3781 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     3841 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgcggcggc
     3901 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     3961 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     4021 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     4081 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     4141 cgagatcggt gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     4201 gccagcgcag gcccccctcg ccaccgcgcg atgcaacgtt ggtatcgccg ttggtagcca
     4261 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     4321 tgaggtaatg cggcaaggcg tggttgatca ccagtggata acctgcttcg gcttcaccgc
     4381 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt cgtgaattat
     4441 tggtacgcag ttcgcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     4501 ttgttggcgt aaccggctgg ttgagctgaa gcaaacggcc aattgccgca catagcagcc
     4561 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     4621 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttccggt gccatttctg
     4681 cactacaggc accaaattca atgaaacggg gtaaccagcc ctgaatcgcc agcgcgctgc
     4741 ggtggaaatc ttccagcgcc atatctttgt gcgcaccgca gttaatgcga tccacgaggc
     4801 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     4861 catcgattaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     4921 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     4981 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     5041 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     5101 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     5161 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     5221 tgctttgtag ctaaggcacg caaggctgtc acgcgatgat taaaaattcg gcgtgtgacc
     5281 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
     5341 tgcttgagcc aggcgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
     5401 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
     5461 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
     5521 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cacgttcgcg cagccactca
     5581 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ctagttccgg acgtgccggg
     5641 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcccgg ggtgatgcag
     5701 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagtggcgc gacgatgatg
     5761 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcgccacg cagtacgccg
     5821 tcagaaccgg tcagcacgtt aacgttgaac tgcctgtcaa tttccagcgc gctcagtacc
     5881 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
     5941 ctgatttcga tgtgattggg gttacgggcc agcgattgca cagcatggct gtcaaagctc
     6001 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
     6061 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
     6121 cgggttaccg ccagcgaagc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
     6181 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
     6241 gtggtcatac gggtagtacc agcgccgatt tttgcagcat cgccaacgcg attaactttg
     6301 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
     6361 tcttcggtaa gcatcacgac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
     6421 gagccgcagc aggctttacc ggtgtagccg ttggcattac cgaattcatc acaggacggg
     6481 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgaccg
     6541 ccgtgagagt ggatctgcac cggttccgcc agcagaccac gggagatctc ttccgccagt
     6601 ggaccgcgta ggccagaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
     6661 agcggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
     6721 ttcgcgatga cgttcatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
     6781 aaggaaaccg tcatgccgtc ctgtaaacct gagcgacgaa tcgcttcttc caggttggcg
     6841 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
     6901 gcggcaagat cgcattcagt gcgacgattc caggccgcta cccgttcttg tcgttgagat
     6961 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
     7021 acgggagagc accagacggg cgcgatcgat aaccggaccg tccaccatct tgccgttcag
     7081 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggtgggc
     7141 gtgatccact tctttctggg tcggtgcgta gaggttgtgc agcagatcaa tctgacgcgg
     7201 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
     7261 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
     7321 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
     7381 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
     7441 agcgtgagcg atttccactg cgcgggtaat gcccagcgga gattcaatcg ccgccagcag
     7501 gccggtgctg ccgggttcac gaccacaggc tttttctata cgcaggatct ctttttcaat
     7561 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
     7621 aacgacggct tccaggtcgt taacacccca ttcggaatcc agcgcgttga cacgcacaat
     7681 ggtttcaata tcgcgataca gcggatgttg cagcgcgtga taaaccatgc ggcgggcggt
     7741 gtctttttca cgtaatgcca cggagtcttc gaggtcaaac atcagggcat cagccgggta
     7801 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
     7861 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
     7921 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcatgtaa aatgcagtcc
     7981 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
     8041 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
     8101 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
     8161 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
     8221 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
     8281 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
     8341 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
     8401 tgggcgctgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
     8461 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacacca agcgcgggag
     8521 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
     8581 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattct gagccgcgat
     8641 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccagc cggtcttcat
     8701 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtg
//
LOCUS       DAJTUN010000144         8711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTUN010000144 DAJTUN010000000
VERSION     DAJTUN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.144"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..2887
                     /locus_tag="OO470_003421"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OO470_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO470_003422"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OO470_003422"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6252550.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OO470_003423"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OO470_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO470_003424"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OO470_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="OO470_003425"
     CDS             complement(7280..8041)
                     /locus_tag="OO470_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR6252551.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="OO470_003426"
     CDS             complement(8038..8697)
                     /locus_tag="OO470_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6252552.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2114 a         2325 c         2266 g         2006 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc t
//
LOCUS       DAJTUN010000145         8615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTUN010000145 DAJTUN010000000
VERSION     DAJTUN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.145"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1074)
                     /gene="trkA"
                     /locus_tag="OO470_003427"
     CDS             complement(<1..1074)
                     /gene="trkA"
                     /locus_tag="OO470_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6252553.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISAL"
     gene            complement(1096..2385)
                     /gene="rsmB"
                     /locus_tag="OO470_003428"
     CDS             complement(1096..2385)
                     /gene="rsmB"
                     /locus_tag="OO470_003428"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6252554.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2431..3378)
                     /gene="fmt"
                     /locus_tag="OO470_003429"
     CDS             complement(2431..3378)
                     /gene="fmt"
                     /locus_tag="OO470_003429"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6252555.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3393..3902)
                     /gene="def"
                     /locus_tag="OO470_003430"
     CDS             complement(3393..3902)
                     /gene="def"
                     /locus_tag="OO470_003430"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6252556.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4050..5174
                     /gene="dprA"
                     /locus_tag="OO470_003431"
     CDS             4050..5174
                     /gene="dprA"
                     /locus_tag="OO470_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6252557.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5146..5619
                     /locus_tag="OO470_003432"
     CDS             5146..5619
                     /locus_tag="OO470_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6252558.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5648..6190
                     /locus_tag="OO470_003433"
     CDS             5648..6190
                     /locus_tag="OO470_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6252559.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6195..6767
                     /gene="tsaC"
                     /locus_tag="OO470_003434"
     CDS             6195..6767
                     /gene="tsaC"
                     /locus_tag="OO470_003434"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6252560.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            6772..7590
                     /gene="aroE"
                     /locus_tag="OO470_003435"
     CDS             6772..7590
                     /gene="aroE"
                     /locus_tag="OO470_003435"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6252561.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7587..7844
                     /locus_tag="OO470_003436"
     CDS             7587..7844
                     /locus_tag="OO470_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6252562.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(7820..8374)
                     /locus_tag="OO470_003437"
     CDS             complement(7820..8374)
                     /locus_tag="OO470_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6252563.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2145 a         2096 c         2163 g         2211 t
ORIGIN      
        1 caacgcagaa atagttgctt gttgtggtga aattgcaata tcgatcacgc tcccctgaac
       61 cagatccaca taagcgcgac gctggatcaa taccatcacc tttttcgcac ccatacgttt
      121 ggcaagcatg gcggacatga tattggcctc gtcatcgttg gtgacggcaa taaacagatc
      181 aacttgatcg atatgttctt cggccagcag ttcttgatcc gacgcatcac caaaaaagac
      241 gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc tgctgattac gttcgatgag
      301 tttaacgctg taatcttttt ccagacgacg cgccagccct gcaccgatat taccgccacc
      361 aaccagcatg atccgtttat acggtttttc cagtcgctgt aattcactca tcaccgcgcg
      421 gatatgctgt gaagcggcaa taaagaacac ttcatcacca gcttcaacaa tggtcgaacc
      481 ttgtggacga atggggcgat cgtggcggaa aattgctgcc acacgagtat cgatatgtgg
      541 catatgttcg cgcatggtcg aaagtgcatt accaatcagc gggccgccat aataggcttt
      601 aactacagcg aggctgactt taccctcagc gaagttcacc acctgcaatg cgcctggata
      661 ctcaatcagt cggtaaatat tatcgataac caactgctct ggtgcgatca gatgatcaat
      721 cggtacagca tctgaatgaa atagcttatc ggcatcgcgt acgtagtctg gtgagcggat
      781 gcgagcgatg cgattagggg tgttgaaaag tgagtaggct acctggcagg caaccatatt
      841 ggtttcatct gaactggtta cagcaaccag catatcggcg tcgtcggcac ctgcctcccg
      901 caatacgcgt ggatgagagc catgcccctg cacgactcgc aggtcaaatt tatcctgcaa
      961 ggtccgcaga cgctcaccgt tggtatcgac aacagtaata tcgttgttct cgccaaccag
     1021 gttttccgcc agtgtgccgc caacctggcc ggcacccaga atgataattt tcatcagtcg
     1081 cgacccgtta tctcatcact ttttgattag cttagcgtaa aagaagccgt cgccctcttc
     1141 ggcaccaggc tgattttgtt tacccggttg ctctggtgtt cctgtttcgc aaagttcggc
     1201 atcagcggta cgttgcaaaa aggctttaat ctgcaggcta ttctcttccg gtaacaccga
     1261 acaggtggca tagaccaggg ttccaccgga ttttaaatgc ggccaaatgg cgtcgagaat
     1321 ttcagactgc aattgcgcga gttccgggat atcgcgatcg cgacgtaacc atttaatatc
     1381 tggatggcga cgaatcaccc ccgttgctga acaaggcgca tctaataaaa tgcgatcaaa
     1441 ctgttgctcg ccacaccatt gggaagggta acggccatca ccttgtttca cggttgcctt
     1501 cataccaagg cgttttaaat tgtcgtaaat gcgagagagg cgctgttcgt caatatcaac
     1561 cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc gttgttttac cgccgggggc
     1621 ggcacaaaga tccaagatgt gttcaccgtt ttgtggcgca agccaggtca tgcaaccttg
     1681 tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa ccaggtagcg catgaacagg
     1741 tgcaggtgtt tccagccgta cagcatcagg gtaatccgca tgcgggaaac ctttcattcc
     1801 tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga tgcgtacgat taatacgcag
     1861 ccacattggc ggacgctggt tattggcttc ggcgatggat tgccactgct ctggatacgc
     1921 tttttgcaga cgcttcagca accaggaagg atgcagataa cgtgcatcac gggtattaaa
     1981 ctcggctaat aactcttctt gccgacgctg gaactggcgt aatacaccgt taatcaaccc
     2041 tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc gtttcagcca gcgcagcatg
     2101 aggtggaatg cgggtataaa gcagttgata caaaccaacc ataatcaggt aatgcacagt
     2161 gcgctgtttg ccggtcatcg gacgggccat taacttatta atcagccagt ctaactgcga
     2221 aagcgtacgc agtacgccaa agcacaactc ttgaagaagt gctttgtctt tatcggaaac
     2281 tttttgctgg agcggtggca gaatgttgct taatgattgc ccttgctcga cgacttgttc
     2341 aacggcctgg gccgccatgc tacgtaaatt acgttgtttt ttcataagta taaaaatgct
     2401 cggcaagacc gggcttagaa gagtggacta tcagaccaga cggttgcccg gaacaaacca
     2461 ttcccgacga gagttcagga ggtcttgcgc gctcatcgct ttcttacccg caggttgtaa
     2521 cgagagcagg ttcaggatgc catcaccagt cgcaacctga atgccttgtt tgttggcttc
     2581 aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg accgacgctt tccagacttt
     2641 aaccggctgt ccttcaattt ccagccagct cattggccat gggttgaaag cgcgaatgca
     2701 gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc gcttcttctt tactcaactt
     2761 ctcggcgtaa gtgacaagag tttcgtcctg aacttctggt ttcgccgtgc cgtctgccaa
     2821 ttgtttcaac gtggtgataa gcccttgtgg gccaagctct gccagcttgt cgtacagcgt
     2881 accactggta tcttctgcag taatcgggca ggagagctta tagagcatgt caccggtgtc
     2941 taaaccaaca tccatttgca taatggttac accagtttct gcatcacccg cccatagtga
     3001 gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt gaaccatgaa tgttgataca
     3061 gccaagacgc ggcatctcca gcacagcttt aggcagaatt aaaccatagg cgacgacgac
     3121 cataacatca gcctgcaaat cggcgaccag ttgctggttt tcttgtggac gcagggaaac
     3181 aggttgaaaa acgggcagac cttcttcctc agccagaact ttaatcgggc tgggcatcag
     3241 ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac acgccaacga cgttatgacc
     3301 agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat
     3361 acgtagtgat tctgacacgt tagttcttat ccttaagccc gggctttcag acgatccagt
     3421 ttttcaactt tctgacgaat tcgttgttgt ttcagcggtg acagataatc cataaacagt
     3481 ttgccaacca ggtgatccat ctcatgctga atacagatag ctaacagacc ctctgcttcc
     3541 agttcaaatg gtttaccgtc gcggtcaagg gcgcgaattt taactttctc tgcgcgcggc
     3601 actaaagcac gttgttcagg gatcgacagg caaccttctt caatgcctgt ttcgccgctt
     3661 ttttctaaaa gctccggatt gattaacacc agccgttcgt cacggttttc cgaaacatca
     3721 ataacaatga tacgttgatg gatatcaacc tgggttgccg ccaggccaat accttcttct
     3781 gcgtacatcg tctcgaacat atcatcgacg atacgctgaa tttctgcatt cacttcttct
     3841 accggtttag caactttgcg aagccgctcg tccggaatat gtaacacttg caaaactgac
     3901 ataaatctcc agagatgtgt tcaggagtta gaaagattat ttcttctatt ctagacaaat
     3961 ccccctctga ttgacagcat cacttgattg acagcatcac tgaccaatcg caaagattgc
     4021 taaggcttgc ttatggcagg gagataagga tggtcgatac agaaatttgg ctgcgtttaa
     4081 tgagcatcag cagcttgtac ggcgatgata tggtccgtat cgctcactgg ctggcaaaac
     4141 agtcgcatat tgatgcggtt gtattgcagc aaacagggct tacattgcgg caggcacaac
     4201 gctttctttc atttccacga aagagtatcg aaagctcact ttgttggttg gagcaaccca
     4261 accatcattt aatccctgcg gacagcgaat tttatcctcc tcaacttcag gcgacgacag
     4321 attaccccgg cgcactgttt gttgaaggag aactgcacgc gctgcattca tttcagcttg
     4381 ccgtagtggg gagtcgggcg cattcatggt atggcgagcg atgggggcga ttattttgcg
     4441 aaactctggc gacgtgtgga gtgacaatta cgagtggact ggcgcgtgga atcgatggtg
     4501 tggtgcataa agcggcctta caggtaaatg gcgtcagcat tgctgtattg gggaatggac
     4561 ttaataccat tcatccgcgt cgccatgccc gactggctgc cagtctgttt gaacagggtg
     4621 gcgctctcgt ctcggaattt cccctcgatg ttccacccct tgcttacaat ttcccacgaa
     4681 gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt ggtggaagcg gctttgcgca
     4741 gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg gcgtgaagtt tttgccttgc
     4801 caggtccaat agggaatccg ggaagcgaag ggcctcactg gttaataaaa caaggtgcga
     4861 ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca atttggattg cactggttgc
     4921 cagacgcccc tgaaaattca ttttattcac cagatcagca agacgtggca ttgccatttc
     4981 ctgagctcct ggctaacgta ggagatgagg taacacctgt tgacgtcgtc gctgaacgtg
     5041 ccggccaacc tgtgccagag gtagttactc aactactcga actggagtta gcaggatgga
     5101 tcgcagctgt acccggcggc tatgtccgat tgaggagggc atgccatgtt cgacgtacta
     5161 atgtatttgt ttgaaaccta tattcacact gaagctgagt tgcgtgtgga tcaagacaaa
     5221 cttgaacagg atcttaccga cgcaggtttt gagcgagaag atatctacaa tgccctgcta
     5281 tggctggaaa aacttgctga ttatcaggaa gggttggcag aaccgatgca actggcctcg
     5341 gatcctctct ccatgcgtat ttatacaccg gaagagtgtg aacgactgga tgccagctgc
     5401 cgtgggtttc tgcttttcct tgagcagatt caggtgctca accttgaaac tcgtgaaatg
     5461 gtgatagagc gagtgctcgc gctggataac gcagagttcg aactggatga tctgaaatgg
     5521 gtgatcctga tggtgttgtt caacatcccg ggctgcgaaa atgcgtacca gcaaatggaa
     5581 gaattactct ttgaagtgaa tgaaggtatg ctgcattaat catttcttta attcagcata
     5641 agttgttatg gcaaaatcag cactgttcac ggtgcgtaat aatgagtcct gcccaaagtg
     5701 cggggctgaa ctggttattc gatccgggaa acacggtccg tttcttggat gctcacagta
     5761 tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg gatggacata ttgtcaaagt
     5821 tctggagggg caggtttgcc ccgtatgtgg cgcaaatctg gtattacgcc agggacgctt
     5881 tggtatgttt attggttgca gtaactaccc tgaatgcgaa cataccgaac ttatcgataa
     5941 accggacgaa acagcaatta catgccccca atgtcggacg ggccatctgg tccagcgccg
     6001 ctcccgttat ggcaaaacat ttcactcctg tgatcgctac ccggagtgtc aatttgccat
     6061 taacttcaaa ccaatagctg gagaatgccc tgagtgtcat tatccgctac tcatcgaaaa
     6121 gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa caatgtggaa agccggtttc
     6181 ggcggaataa taacgtgaat aataacctgc aaggagacgc tatcgcagct gcgatagatg
     6241 tcctcaatga aaaacgtgtc atcgcctatc caacggaagc cgttttcggt gtcgggtgcg
     6301 atcctgatag cgaaacagca gtgatgagac tgttggagtt aaaacagcgt ccggttgata
     6361 aggggctgat tttaatcgcg gcaaattacg agcagcttaa accctatatt gatgacacca
     6421 tgttgactga cgtgcagcgt gaaaccattt tttcctgctg gccaggtcct gtcacctttg
     6481 tctttcccgc gcctgcgaca acaccgcgct ggttgacggg ccgctttgat tcgcttgctg
     6541 tacgagtcac cgaccatccg ttggtggttg ctttgtgcca ggcttatgat aaaccgctgg
     6601 tttctaccag tgccaacttg agtggattgc caccttgtcg aacagtagac gaagttcgcg
     6661 cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac gggggggcgt ttaaatcctt
     6721 cagaaatccg cgatgccctg acgggtgaac tgtttcgaca ggggtaacat aatggaaacc
     6781 tatgctgttt ttggtaatcc gatagcccac agcaaatcgc cattcattca tcagcaattt
     6841 gctcagcaac tgaatattga acatccctat gggcgcgtgt tggcacccat caatgatttc
     6901 atcaacacac tgaacgtttt ctttcgtgct ggtggtaaag gtgcgaatgt gacggtgcct
     6961 tttaaagaag aggcttttgc cagagcggat gagcttactg aacgggcagc gttggctggt
     7021 gcagttaata ccctcaagcg gttagaagat ggacgcctgc tgggtgacaa taccgatggt
     7081 gtaggcttgt taagcgatct ggaacgtctg tcttttatcc gccctggttt acgtattctg
     7141 cttatcggcg ctggtggagc atctcgcggc gtactactgc cacttctttc cctggactgt
     7201 gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag agttggctaa attgtttgcg
     7261 cacactggca gtattcaggc gttgggtatg gacgaactgg aaggtcatga gtttgatctc
     7321 attattaatg caacatccag tggcatcagt ggtgatattc cggcgatccc gtcatcgctc
     7381 attcatccag gcatttattg ctatgacatg ttctatcaga aaggaaaaac tccttttctg
     7441 gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg gtttaggaat gctggtggca
     7501 caggcggctc atgcctttct tctctggcac ggtgttctgc ctgacgtaga accagttata
     7561 aagcaattgc aggaggaatt gtccgcgtga atcaggccat ccagtttccg gacagggaag
     7621 agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct cgtgaatggt atgcaactga
     7681 catgcgcgat ctctggcgag agtctggcgt atcgctttac tggagatacg ccagaacagt
     7741 ggttagcgag ttttcgtcag catcgctggg acctggaaga agaagcggaa aacttaattc
     7801 aggaacaaag tgaagatgat caaggctggg tctggttacc ctgatccaga tattcgtcct
     7861 tccatttcac gtaattattc gcggaatagc gtaacccagc cttctcttca tcacttaacg
     7921 ggcggatctg tttgacgggg ctaccgagat acagatatcc gctctccagc cgtttatttt
     7981 gtgggaccag actacccgca ccaatcatca catcatcttc tactattgcg ccatcaagta
     8041 aaattgagcc catcccaacc aaaactcgat tgccaatggt gcagccgtgg agcatcacct
     8101 tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt gccatctggg ttgtacgagg
     8161 atttatgagt gacatgcaac atactgccat cctggatatt ggtgcgtgct ccgatctgta
     8221 cataatgtac atctccacga atcacaacga gcggccagat ccccacatca tcagccagac
     8281 gaacgtcacc aatcacgaca ctgctatcgt cgatcattac gcgctgaccg atttgtggaa
     8341 aaagatcgcg gtatgggcgt aaaacatcag acatacttac ctcagcaata aatgatttac
     8401 taatgacttt gggggcatta ttggccttgt gcaagtcttt tagtatgcaa aaaagcaccg
     8461 ttttgtgtgc gattgcagca aaaagggtga aaaaacaaca aacagaaaaa aagatcaaaa
     8521 aaatacttgt gcaaaatatt gggatcccta taatgcgcct ccgttgagac gacaacgtga
     8581 aacacttcac aggatggtcg gaacaacgaa gagaa
//
LOCUS       DAJTUN010000146         8495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTUN010000146 DAJTUN010000000
VERSION     DAJTUN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.146"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OO470_003438"
     CDS             complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="OO470_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6252564.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO470_003439"
     CDS             complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO470_003439"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6252565.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2165..3199
                     /gene="rhaT"
                     /locus_tag="OO470_003440"
                     /pseudo
     CDS             2165..3199
                     /gene="rhaT"
                     /locus_tag="OO470_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO470_003441"
     CDS             complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO470_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6252566.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO470_003442"
     CDS             complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO470_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6252567.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5242..6711
                     /gene="rhaB"
                     /locus_tag="OO470_003443"
     CDS             5242..6711
                     /gene="rhaB"
                     /locus_tag="OO470_003443"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6252568.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6708..7967
                     /gene="rhaA"
                     /locus_tag="OO470_003444"
     CDS             6708..7967
                     /gene="rhaA"
                     /locus_tag="OO470_003444"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6252569.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8209..8495
                     /locus_tag="OO470_003445"
                     /pseudo
     CDS             8209..8495
                     /locus_tag="OO470_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
BASE COUNT         2052 a         2227 c         2225 g         1991 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct tctttcgtac
      601 cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca
      661 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
      721 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg
      781 atttacgcag taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
      841 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
      901 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
      961 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
     1021 tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata
     1081 gacattcatt taatcaataa aagtgatcaa aatcacatca attgaaacgc tgttttattt
     1141 ttataagacc attgttttta tttttaaatt gatcacaaaa aaaccaccgc cgttggcgat
     1201 ggttcatttt ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaatgat
     1261 tattttttcg ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct
     1321 ttgatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc
     1381 aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc
     1441 tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg
     1501 gaaccaaagc gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg
     1561 aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct
     1621 ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg
     1681 tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct
     1741 ggcaggcttt ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg
     1801 tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg
     1861 gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc
     1921 gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg
     1981 tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca
     2041 cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac
     2101 agcacgattt ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa
     2161 gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc
     2221 agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc
     2281 agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc
     2341 ggatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt
     2401 cggcgctatg tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat
     2461 gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc
     2521 aattatcaac ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttact
     2581 cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga
     2641 gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt
     2701 gatgtgcggc attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca
     2761 tgaagccgct gccgcacttg gcgtcgatcc actgtatgtt gctctgccaa gctatgttgt
     2821 catcatgggc ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt
     2881 gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt
     2941 gttactctcg gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctaggg
     3001 ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta
     3061 tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg
     3121 tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg
     3181 catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat
     3241 cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt
     3301 cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct
     3361 gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa
     3421 aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag
     3481 gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa
     3541 cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc
     3601 agccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct gaccgataac
     3661 ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc
     3721 gctaaatccc ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata
     3781 aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg
     3841 aatataaaag agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc
     3901 attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc
     3961 ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt
     4021 gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt
     4081 cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc
     4141 cctggcgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt
     4201 tactgtcgct gaatccacag cgataggcga tgtcagtgac gctggcctcg ctgtggcgta
     4261 gtagatgtcg ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg
     4321 tttgctgctt aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg
     4381 catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac
     4441 gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt
     4501 gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt
     4561 ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag
     4621 acggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa
     4681 atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca
     4741 gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc
     4801 cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat
     4861 gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttacaag
     4921 acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg
     4981 gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa aactacgtaa
     5041 aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc
     5101 accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
     5161 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
     5221 gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca
     5281 tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc
     5341 gaaatccatc gttttaacaa tgggctgcat agccaaaacg gttatgtcac ctgggatgtg
     5401 gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt
     5461 atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt
     5521 cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatgccgcag
     5581 gcacaacagc aactcggcaa acgcgatatt tatcagcgta gcggcatcca gtttctaccc
     5641 ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact tattccacac
     5701 attgctcacg ctctgctgat gccggattac ttcagttatc gcctgaccgg caagatgaac
     5761 tgggaatata ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac
     5821 gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg
     5881 ggtaatgtca ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt
     5941 gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct
     6001 tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat
     6061 gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg
     6121 ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac
     6181 gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat
     6241 cccaatgacg atcgctttat taatcctgac gagatgtgca gcgaaattca ggctgcgtgt
     6301 cgggaaacgg cgcaaccgat cccgggaagt gatgctgaac tggcgcgctg tattttcgac
     6361 agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat
     6421 ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc
     6481 gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat
     6541 atcggcatcg agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc
     6601 gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac
     6661 tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac
     6721 tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg
     6781 aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg
     6841 tttccggttt tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggcaatt
     6901 atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc
     6961 tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag
     7021 tctcgcgcga ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca
     7081 atcagctcgg tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct
     7141 ttacgctctc gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca
     7201 gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct
     7261 ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc
     7321 tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg
     7381 ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt
     7441 acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc
     7501 cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtaccg cagttgctgc
     7561 tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa
     7621 cccaggcaat tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc
     7681 ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata
     7741 tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag
     7801 cggcgggcga ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgcggc
     7861 aggcggtctg ggaaatgtat tgccaacgtc acgatacgcc agcaggtagc gaatggctgg
     7921 agagcgtgcg ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg
     7981 cggcacgagc gccttatccg gcctacgggt cgaatgtcca tgcgggctgt caccctcacc
     8041 ccggccctct ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg
     8101 aacaccgctc cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa
     8161 gccgcaccat gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt
     8221 acccagtcct ggtttgtcca gggaatgatc aaagccacca ccgatgccta gctgaaaggc
     8281 tgggacgagc gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgctt
     8341 tatcacgaca acttccaccc acaaccgcgc tatattccgc tcagccaacc catgccttta
     8401 ctggcaaata cgccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctc
     8461 gatcctgcag ctaacttagg cgtcgtaaaa gtcga
//
LOCUS       DAJTUN010000147         8491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTUN010000147 DAJTUN010000000
VERSION     DAJTUN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.147"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..756)
                     /locus_tag="OO470_003446"
     CDS             complement(<1..756)
                     /locus_tag="OO470_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6252570.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQ"
     gene            complement(857..1117)
                     /gene="iraP"
                     /locus_tag="OO470_003447"
     CDS             complement(857..1117)
                     /gene="iraP"
                     /locus_tag="OO470_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6252571.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(1299..1502)
                     /locus_tag="OO470_003448"
     CDS             complement(1299..1502)
                     /locus_tag="OO470_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252572.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            1580..2674
                     /gene="ddlA"
                     /locus_tag="OO470_003449"
     CDS             1580..2674
                     /gene="ddlA"
                     /locus_tag="OO470_003449"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6252573.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(2698..2910)
                     /locus_tag="OO470_003450"
     CDS             complement(2698..2910)
                     /locus_tag="OO470_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6252574.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            3170..3478
                     /locus_tag="OO470_003451"
     CDS             3170..3478
                     /locus_tag="OO470_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6252575.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(3537..4631)
                     /gene="yaiW"
                     /locus_tag="OO470_003452"
     CDS             complement(3537..4631)
                     /gene="yaiW"
                     /locus_tag="OO470_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6252576.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(4644..5864)
                     /gene="sbmA"
                     /locus_tag="OO470_003453"
     CDS             complement(4644..5864)
                     /gene="sbmA"
                     /locus_tag="OO470_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6252577.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6216..7373
                     /gene="ampH"
                     /locus_tag="OO470_003454"
     CDS             6216..7373
                     /gene="ampH"
                     /locus_tag="OO470_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6252578.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(7374..7877)
                     /locus_tag="OO470_003455"
     CDS             complement(7374..7877)
                     /locus_tag="OO470_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6252579.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(7965..>8491)
                     /locus_tag="OO470_003456"
     CDS             complement(7965..>8491)
                     /locus_tag="OO470_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6252580.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2145 a         2152 c         2015 g         2179 t
ORIGIN      
        1 ctgtgcctgt tcacgcagcg tttttccctg ccattcacca gcggttgccg tttcagcaaa
       61 ggtttttgcg ccgccgccaa gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat
      121 cgatcctttt ccgccttttt ccagagcgtt acccggacat ttttcactgg tcgcgctcgg
      181 accgtagcat ttgcgcgaag tcacatgtgc caccagcgca gcgggcgtgg catcctgcaa
      241 ctctgcggta gaaacgttac cggtcgccag acctgcggct tttgccattt ccagaatcgt
      301 tgggtgatct ttttcgtgaa tatcgacgcc cagcgcgccg ttataggttt tgacaccggt
      361 tgaccaggcg gttgctgatg cagccgagtc ggtgacgtag tccggtttgc cagttttttt
      421 attcagcgca tagtgagtgt attgcccggt aagcggtaag gcatcgatac ctttaaaaaa
      481 gccgcccgca ccttcggcat aattacgtgc ggcagtaatt tccgaatccc ccatcccatc
      541 gccaatcagc aaaataatat tttttgcagg tttatcgcta agagaatcac gcagagcggc
      601 ggtctgatca cccgttaagc ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc
      661 ccggttttcc agaacaggca tttctggtgt ccgggctttt gtcacagggg taaacagtaa
      721 cggtaagagt gccagtgcaa tagtgctttg tttcacttta ttttctccat gtacaaatac
      781 attaaaaaat aaaaacaaag cgactataag tctcggccgt gacaacttta tgacagctgt
      841 tgaaaagatt aactttttac tgacaaggat gcttcaataa cttctttacg taatcgcgca
      901 gcagctctgt atcgtcgtca ggaatgctgg catcgggctt tacctcgtac agcgccccct
      961 ctacctgatc aatcaaccgc tgttggtcat tttgcgccat attgcgaagc attgcagtga
     1021 cgataatctc caaagcttct acctgcgcac acagttcttt cgactcttct tctttttggg
     1081 caagcttaaa taacaactca gcaatgagat ttttcatgtc tgtatttcct tatccaaagt
     1141 atggagaagt taacattatg tttcacaact tcatagtgtc tggaagtatt atttttatga
     1201 caaaggaaat actttgcgca aatatttttt gtttgattac cagcgaaacg tttctcttat
     1261 ttaaataatt acccacacaa aatataggct tttaattatc aacctttatt ataaataaaa
     1321 gctatgaaat atattctctt cgttaactta taattaagag aaaaaaactc accgcattgc
     1381 catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt gcgcacatct
     1441 ttgagataac gcactttctt caccagtacg ttgcacgctt tgtcagcaga agattgcaac
     1501 aatctttcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta
     1561 gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg gtggtaaatc
     1621 agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag
     1681 tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg tcagcgatgc
     1741 cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc cttcggcgac
     1801 cagccttgcg caggtgccag gtaaacatga gcatcagctt atcgacgcgc aaaacggtca
     1861 gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg gcgaagatgg
     1921 ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg atgttctggc
     1981 ttcagcagcc tgtatggata aagatgtcac caaacgtctg ctacgcgatg ccgggctgaa
     2041 cattgcgcca tttattaccc tgacgcgcgc taatcgtcac aacatcagtt ttgccgaagt
     2101 ggagtctaaa ctggggttac cactgtttgt aaaaccggct aatcagggct cttctgttgg
     2161 tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc gtcgatctgg cgttcgagtt
     2221 cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat gcgcagttct
     2281 gggcaacgac aatccgcaag ccagcacctg tggcgagatc gtactcacca gcgatttcta
     2341 tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat
     2401 tgcaccagag atcaacgata agatccgggc gattgccgtt caggcttatc agacgttggg
     2461 atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag tggtgatcaa
     2521 cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc tgtggcaagc
     2581 cagcggtctg ggttacaccg atctgatcac acgtttgatt gagctggcgc tggagcgtca
     2641 cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat aatattctta
     2701 ttgggcgttt tcagtttctt catcgcgacg acgaataatc aaccccgcca gccagaaact
     2761 aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt caaatcccag
     2821 tacccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat agtagtgcca
     2881 gtcacggcgg atttttacag gcagattcat ggcagctccg ttaacgaaaa acaacatcct
     2941 tgcatattct gtaacaaccg tctgtggtgt agacaacatt ttcgtaatta ttaataaatt
     3001 taacttaagg ttgccatttt gtgactcaca acgaagattc acgcttcggg aatattccca
     3061 acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga cttgccataa
     3121 gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta tggctgattt
     3181 caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa
     3241 cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat tgctgaacct
     3301 gttagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa caggtcgccc
     3361 acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt ttttagcagg
     3421 ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc gccagtaaaa
     3481 tttacgttgc cagatgcggc ctgaaacgcc ttatctggcc tgacattcct gtttgatcac
     3541 tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc cgtagtcagg
     3601 ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg gggtagtgat
     3661 ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt ctcctcaaaa
     3721 gagaagctgt cacctttctc taactgacgg cgaatttcgc tgtcgctcat ccccagtttt
     3781 cccgccagtt tgcgcgttgc cagttcggtt ttcctgggtt ctttactgtc gtaacgaatt
     3841 aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgactgcgtt ctgaaacgcg
     3901 gcattacggc tggcgtacca gccagcgtta aaatcagcaa agcggtatat cggtgcgcta
     3961 tagctggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc gcggcgactg
     4021 aagacttcct gacgaactgt accgtccatt ttccacggat accctttggt atgctgttcg
     4081 gcaaaagcaa tgctgacctg catcggccca ccggtgcgca ccggattaag cgaaccaaac
     4141 agcgtctgcc ccattggtac catgctgatc agatcgtcaa aaatcgcgct caattgcttt
     4201 tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga tttgattttt
     4261 agcgcggtat gaaccagaaa agcaggaatg tgcatccgtt cagcacgacg gtcaatctct
     4321 tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt tgactcctgc
     4381 tgcgccaccg ccagcaccga gtagacgttt tccagcgtcg gtgccagccc ctgactttca
     4441 aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc
     4501 tgccgcacga cgctcgctac atctatcgct ttttcgccct ttttcaacgg ttgcggagcc
     4561 tgactactac accccgctaa aaccagaacc gccaacaatg acagcgaaga tagatgattg
     4621 acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg aattttgtca
     4681 ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt
     4741 gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta
     4801 atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac
     4861 aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatgttgaa atacatatag
     4921 tgaaaataga ggcgaaaata gtttttccgt acggcgctaa acagctcgcg taccgtaggc
     4981 ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta ggcggcctct
     5041 actcgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc cagcaatccg
     5101 gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat gtgcccgata
     5161 atcggcagct ccggcacatg cgcggagagc gttaccagca ccggcaggaa ggcgatcaac
     5221 gtcattatgg cgttgataaa actgacgccc atattctcca gcgttgaagc aaaacgcatg
     5281 gtgtcttcct gcacacgctg tgcggcacct tcgatatgac gcagttgttg ccagttcgcc
     5341 atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac aaagaagttg
     5401 ttcagtacac tgatcaccac tgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg
     5461 taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat cagatcatag
     5521 aacggcgcat accaggcgtt gacggcgacc ccgacttcca ccaaaaacca agtgacgaag
     5581 ataatcagcg cagtaccgag tattgaccag tattgccaac ggtgcgggct gtagataaac
     5641 cagaaaaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc
     5701 aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt gatatgtgcc
     5761 acccagtcac ccccaccggc ttgccagaag ataacggcaa tcaatgccca aacaaaggcc
     5821 gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa acatatgcac tcctgctaac
     5881 ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca
     5941 aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct gaccataaga
     6001 attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta acacgcgcag
     6061 gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt
     6121 ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag tataaatacg
     6181 ctcagttacc ttcattcaat ctatggacac caccgttgaa acgtagtctg cttttttctg
     6241 ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc actgaaccgg
     6301 agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc agcggtgcca
     6361 cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgtagt tatggcgaaa
     6421 cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt gcttccctca
     6481 ccaaactaat gaccagtgaa atgctggtga aattgctcga ccaggggacc gtaaagttaa
     6541 acgatccgct aagtaaatat gctccgccag gcgcacgcgt gccgacctac aatgggacgc
     6601 ccattaccct agtgaatctg gcaacccata ccagcgccct gccccgtgaa cagcccggtg
     6661 gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg aaataccttt
     6721 ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac cttgcgttcg
     6781 acctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag
     6841 agcagattac ccatccattg gggatgaaag acaccaccta caccccctca ccggatcagt
     6901 gccgccgttt aatggttgct gagcgcggtg ccagtccgtg caataacacg ctggcggcaa
     6961 ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg cagcagtatt
     7021 tgtcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg ttgatttatc
     7081 agcgcgcgca gtttacgaaa gtgattggca tggatgtccc cggcaaagcg gatgcgcttg
     7141 gtttaggctg ggtatatatg gccccgaaag agggtcgtcc gaggattatt cagaagacag
     7201 gcggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat attggcgcgt
     7261 ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg
     7321 atctggtgac cgagctaagc gggaataaac cgttggttat ccccgcatcc tgattaatac
     7381 tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat atagccgcct
     7441 ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg
     7501 atataattca tcacaccaat acgtgatcgc aattcttcat tccagtcaat cattgataat
     7561 aatgttgcgc gggtttgttt ggctggtaga tgatatcccg tacaatttcc ttctgatatt
     7621 aatttgtatg gtatatcgtt tttcattaaa ccatcagcca gccccataat ataaggagcc
     7681 tgggtaatac cgataagtac gttttcttct ctacgcagag agataactcc ttctaaaata
     7741 acgaaagtat cttcgttatt gacatcactg gaaaatataa cttgcttttc attattaaac
     7801 tcgaagcgcg taccgtatct ggacaaacat ttatcgagct taccaaattc ctgaagaggt
     7861 ttaactacag ataacatttg cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc
     7921 attatttaga ttaaattacc agtatttctt cggagtgaag aatattacca agtatattta
     7981 acacccacgt tcgcggacca gtcttgatct acgtcaccac caccgaggta gttagcatcg
     8041 gtataggcgc tgaagttctt ggtgaagctg aactgagtac ccagaccaac acgtaccgca
     8101 gacccttcag taccgttatc gatggaatca ccgttcacat cgttatcgtt gttagagtcg
     8161 tcgtagacgt aagccagttt gaagtacgga gtcagagcct gatcttcgct gtaggtgaag
     8221 gtataacctg catctacacc cagttcataa cgcatgctgt cgtaaggctg accgtcaact
     8281 ttcatgtcgt tgctcagctg gtagtcatca ccagactgga acagaccaga aatgctgccg
     8341 taaggagtca cgtaaccagc atcacccagt ttgaagtcgt aaccggtttt caaaccgaag
     8401 ccccaagcgt cggagttggt gctaccgtca acgtaagtac cgttgctcat ggttgcagac
     8461 aggtcgttgt tgaagtgaga gtagctcaag c
//
LOCUS       DAJTUN010000148         8466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTUN010000148 DAJTUN010000000
VERSION     DAJTUN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.148"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..892
                     /locus_tag="OO470_003457"
     CDS             <1..892
                     /locus_tag="OO470_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6252581.1"
                     /translation="PSLEQWTLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALE
                     KLDLKLAENKDTCWLKSIFRKHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHS
                     ITTRPVSQSVQPNSEDLSVKHKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWL
                     VHWNQQHQQERLAPAPRVDWNQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPN
                     GTSLAKNLQKLPLVALCLKRYSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATL
                     SKERITEEAQRFLEMVYGEE"
     gene            893..2509
                     /locus_tag="OO470_003458"
     CDS             893..2509
                     /locus_tag="OO470_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6252582.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(2565..2717)
                     /locus_tag="OO470_003459"
                     /pseudo
     CDS             complement(2565..2717)
                     /locus_tag="OO470_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2740..3117)
                     /locus_tag="OO470_003460"
     CDS             complement(2740..3117)
                     /locus_tag="OO470_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252583.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(3527..3898)
                     /locus_tag="OO470_003461"
     CDS             complement(3527..3898)
                     /locus_tag="OO470_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252584.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(3959..4456)
                     /locus_tag="OO470_003462"
     CDS             complement(3959..4456)
                     /locus_tag="OO470_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252585.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(4532..5320)
                     /gene="aadA1"
                     /locus_tag="OO470_003463"
     CDS             complement(4532..5320)
                     /gene="aadA1"
                     /locus_tag="OO470_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6252586.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(5378..5902)
                     /gene="sat2"
                     /locus_tag="OO470_003464"
     CDS             complement(5378..5902)
                     /gene="sat2"
                     /locus_tag="OO470_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6252587.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(5997..6470)
                     /locus_tag="OO470_003465"
     CDS             complement(5997..6470)
                     /locus_tag="OO470_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6252588.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            6802..7338
                     /locus_tag="OO470_003466"
     CDS             6802..7338
                     /locus_tag="OO470_003466"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6252589.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            7453..7779
                     /locus_tag="OO470_003467"
     CDS             7453..7779
                     /locus_tag="OO470_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6252590.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(7967..8206)
                     /locus_tag="OO470_003468"
     CDS             complement(7967..8206)
                     /locus_tag="OO470_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6252591.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         2311 a         1856 c         1997 g         2302 t
ORIGIN      
        1 cccaagcctt gagcagtgga cgctgtttta tcagcgctta gcgcaggatc tagggctaac
       61 caaaagcaag cacattcgtc atgacttggt ggcggagaga gtgaggcaga cttttagtga
      121 tgaggcacta gagaaactgg atttaaagtt ggcagagaac aaggacacgt gttggctgaa
      181 aagtatattc cgtaagcata gaaaagcctt tagttattta cagcatagta ttgtgtggca
      241 agccttattg ccaaaactaa cggttataga agcgctacag caggcaagtg ctcttactga
      301 gcactctata acgacaagac ctgttagcca gtctgtgcaa cctaactctg aagatttatc
      361 tgttaagcat aaagactggc agcaactagt gcataaatac caaggaatta aggcggcaag
      421 acagtcttta gagggtgggg tgctatacgc ttggctttac cgacatgaca gggattggct
      481 agttcactgg aatcaacagc atcaacaaga gcgtctggca cccgccccta gagttgattg
      541 gaaccaaaga gatcgaattg ctgtacgaca actattaaga atcataaagc gtctagatag
      601 tagccttgat cacccaagag cgacatcgag ctggctgtta aagcaaactc ctaacggaac
      661 ctctcttgca aaaaatctac agaaactgcc tttggtagcg ctttgcttaa agcgttactc
      721 agagagtgtg gaagattatc aaattagacg gattagccaa gcttttatta agcttaaaca
      781 ggaagatgtt gagcttaggc gctggcgatt attaagaagt gcaacgttat ctaaagagcg
      841 gataactgag gaagcacaaa gattcttgga aatggtttat ggggaagagt gagtggttag
      901 gctagctaca tttaatgaca atgtgcaggt tgtacatatt ggtcatttat tccgtaactc
      961 gggtcataag gagtggcgta tttttgtttg gtttaatcca atgcaagaac ggaaatggac
     1021 tcgatttact catttgcctt tattaagtcg agctaaggtg gttaacagta caacaaagca
     1081 aataaataag gcggatcgtg tgattgagtt tgaagcatcg gatcttcaac gagccaaaat
     1141 aatcgatttt cctaatctct cgtcctttgc ttccgtacgc aacaaggatg gagcgcagag
     1201 ttcatttatt tacgaagctg aaacaccata tagcaagact cgttatcaca tcccacagtt
     1261 agagctagct cggtcattat ttttaattaa ctcctatttc tgtcgaagct gtttgagcag
     1321 taccgcttta cagcaagagt tcgacgttca gtatgaggtt gagcgagatc atttagagat
     1381 aaggatctta cccagttcat cgtttcctaa aggggcgtta gagcagtcgg ccgtagtgca
     1441 gcttttggtt tggttgtttt cggatcaaga tgttatggat tcgtatgaaa gtatttttag
     1501 gcactatcaa caaaatagag aaattaagaa cggcgttgaa agctggtgct ttagctttga
     1561 ccctccgccc atgcagggtt ggaaattaca tgtaaaagga cgttcttcta acgaggataa
     1621 ggattattta gttgaggaaa tagtaggttt agaaatcaac gctatgcttc ctagcacaac
     1681 agctattagc catgcctctt ttcaggaaaa ggaggcaggt gatggtagta cgcagcacat
     1741 agcggtttca acagagtcag ttgttgatga tgagcatcta cagttggacg atgaggaaac
     1801 agccaatata gacacagaca cacgagtcat agaggctgag ccgacatgga taagttttag
     1861 tagacctagt cgaattgaaa aatctcgcag ggcaagaaaa agtagccaaa ctattttaga
     1921 aaaagaagaa gcaacaacaa gtgaaaatag taatttggtt agtactgatg agccacactt
     1981 aggtggtgtc ctagcagcgg cagatgtggg tgggaagcag gatgcaacca attacaactc
     2041 tatttttgct aatcgatttg ctgcttttga tgagctactt tcaattctaa aaactaaatt
     2101 tgcatgtcgg gtgctttttg aagaaacctt ggttttgcca aaagttgggc gtagccgatt
     2161 acatctgtgt aaagatggct caccaagagt gattaaagcc gttggggtgc aacgtaatgg
     2221 cagtgaattt gtattgctag aggtggatgc atcggatggg gtgaaaatgc tttctaccaa
     2281 agtgttgagt ggcgttgata gcgaaacatg gcggaatgat tttgaaaaga tacggcgtgg
     2341 agtggtgaag agctcattga attggccaaa tagtttgttt gatcaattat atggacaaga
     2401 cgggcataga ggggtgaatc atccaaaggg gttgggggag ctgcaagtat cgagagagga
     2461 tatggaaggg tgggctgaga gagtggttag agagcaattt acgcattaaa ggaatgactg
     2521 aaagagcctg taaacccttt tgtgtaagtg cttttgccgg tcagttaaag gtggccattt
     2581 aaacggtcac caaattcgat cataaaacgg ttcatggccg gcttccagtt gcggatcggc
     2641 atcgtccatt tcttggtcgc cgcctggata gccaggtaca ccaccttcat cgctgattcg
     2701 tccgtaggga acaccttgag ttcatattaa tggaattttc tacaaatagc ctccgtggtt
     2761 ttgagggggg attacagacg atccatagta gtaatccaat gagttcttga gcgcggcgac
     2821 atgtttggac gccttggcaa aaattagagc ctgcttgaag tgcaggcgag cacgtgcttg
     2881 gacgattgat ccattcgcgg tcaaaaactc aatcttggat gacagcgtgt caggaaatcc
     2941 aatatcgaag tcccgccccg tgtcaaagta gggcatttca tgatcaaagg acgaagcttc
     3001 ccaggccact tccctaatat tccagccgct agaaacaaga tttcgtacga cttcatcaac
     3061 ttttttgctt tccaacttgt cacaaagtgc ataggcaaga gctttggcgg cagacacgtg
     3121 cgtttcctct taaacatagg tcgaacggat acccgaggca aagcggagca ttagtcccaa
     3181 agcatctccg gtagatccgg aggctccata ctgaaggcat ggcatcttgg gcattgatat
     3241 ttgaaacttc tctctagata gcagggattt cttggccttg cctcatgctg atagcttgta
     3301 aatatcacac gatccttagc cccacaaagt gagcatactc cgtagctttc atttatagaa
     3361 atcgtcgtta gattgtcgca gcatgtgcac gttccgacta tttgctcagg aagcatcgcc
     3421 ataccttggc ctaggtaaag aagatcagac tcgtagccac atcggtggca actgagcttg
     3481 tacgcaatgc ccatctggtt gtctcacctg aatcgtaacg tcagagctat tcatccatcc
     3541 attcggcatc aatgtatgcc actaagccga ccagctgacc gtttggactg aagtatgttg
     3601 cctgcacccc acttgacatc tcatcaatac caccgcactg cgctaggcgc cggtcccact
     3661 tgcccacaga agcgtcagga tcggctgagt tccccaagac ccttgtgagc catgcctcct
     3721 ttaactcaac ctctcgaatg attgcgtcgg ccagctcctc cgcgttatct agttcgacat
     3781 agtctggaat tcggacgtac gttctgaaca cagctggata cttacttggg cgattgtcat
     3841 acatgacatc aacaatgtac ccgtttgtgt aaccgtctct tggaggttcg tatgacacta
     3901 gtggttcccc tcagcttgcg actagatgtt gaggcctaac attttattag agagcaggct
     3961 agttgcttag atacatgatc ttcaggccgt tatctgtcag ggcaagcgaa aattggccat
     4021 ttatgacgac caatgccccg cagaagctcc catctttgcc gccatagacg ccgcgccccc
     4081 cttttggggt gtagaacatc cttttgccag atgtggaaaa gaagttcgtt gtcccattgt
     4141 tggcaatgac gtagtagccg gcgaaagtgc gagacccatt tgcgctatat ataagcctac
     4201 gatttccgtt gcgactattg tcgtaattgg atgaactatt atcgtagttg ctctcagagt
     4261 tgtcgtaatt tgatggacta ttgtcgtaat tgcttatgga gttgtcgtag ttgcttggag
     4321 aaatgtcgta gttggatggg gagtagtcat agggaagacg agcttcatcc actaaaacaa
     4381 ttggcaggtc agcaagtgcc tgccccgatg ccatcgcaag tacgaggctt agaaccacct
     4441 tcaacagatc gcgcatagtc ttccccagct ctctaacgct tgagttaagc cgcgccgcga
     4501 agcggcgtcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
     4561 ctttcacgta gtgaacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     4621 gtccaagata agcctgccta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
     4681 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
     4741 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
     4801 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
     4861 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
     4921 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
     4981 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
     5041 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
     5101 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
     5161 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
     5221 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
     5281 atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac tcctgaatta
     5341 agccgcgccg cgaagcggtg tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg
     5401 agaaccagta ccagtacatc gctgtttcgt tcgagacttg aggtctagtt ttatacgtga
     5461 acaggtcaat gccgccgaga gtaaagccac attttgcgta caaattgcag gcaggtacat
     5521 tgttcgtttg tgtctctaat cgtatgccaa ggagctgtct gcttagtgcc cactttttcg
     5581 caaattcgat gagactgtgc gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt
     5641 gttcgataga ggctagatcg ttccatgttg agttgagttc aatcttcccg acaagctctt
     5701 ggtcgatgaa tgcgccatag caagcagagt cttcatcaga gtcatcatcc gagatgtaat
     5761 ccttccggta ggggctcaca cttctggtag atagttcaaa gccttggtcg gataggtgca
     5821 catcgaacac ttcacgaaca atgaaatggt tctcagcatc caatgtttcc gccacctgct
     5881 cagggatcac cgaaatcttc atatgacgcc taacgcctgg cacagcggat cgcaaacctg
     5941 gcgcggcttt tggtacaaaa ggcgtgacag gtttgcgaat ccgttgctgc cacttgttaa
     6001 cccttttgcc agatttggta actataattt atgttagagg cgaagtcttg ggtaaaaact
     6061 ggcctaaaat tgctggggat ttcaggaaag taaacatcac cttccggctc gatgtctatt
     6121 gtagatatat gtagtgtatc tacttgatcg atcaggcttt tgtatatctc cccaccacct
     6181 gaaacaatga catgatccgt tattttcttt aggttggtta aagcatcttt aattgatgga
     6241 aagatcaata cgttctcatt gtcagatgta aaacttgaac gtgttacgac cgcatacttt
     6301 cggttgggta atgctcccat tgattcaaaa gtcttgcgtc caaccaacag ccattggtta
     6361 taggtaatag ctttaaacag gagctgttca cctttggcac tccatggaat atcagggcca
     6421 ttcccgataa ctccattctt cgatatagct accattagtg atagtttcac aattcttcct
     6481 cagaggttaa cattttatta gtaagcatgc tcgttttgtc acccgctgat gcttaccgtt
     6541 aattaatata atcacttgta ttaaatagac tttctttaaa aatacaagac actctgttat
     6601 tacaaatcgt gcatgccgtc tatcctgctt gcacgatgca cattatctca cccaaagaac
     6661 tcataacatt ttaatttatt tagcattaaa ttaaaactct accggtaaac aagcatctct
     6721 aggcgtaaaa aacaggcact tttttaaatc ccagctagaa taggctgtat aggcagacag
     6781 ttgcaagaca aggagcgttt tatgtctaac agtccatttt taaattctat acgcacggat
     6841 atgcgacaaa aaggttatgc gctgaaaact gaaaaaactt acctgcactg gattaagcgt
     6901 tttattctgt ttcacaaaaa acgtcatcct cagaccatgg gcagtgaaga ggtcaggctg
     6961 tttttatcca gcttagcaaa cagcagacat gtagccataa acacgcagaa aatcgcttta
     7021 aatgccctag cttttttgta caacaggttt ttacaacagc cgttgggcga tattgattat
     7081 atccctgcaa gcaagcctag acggctaccc tctgttatct ctgcaaatga agtgcaacgc
     7141 attttgcagg ttatggatac tcgcaaccaa gttattttta cgctgctgta tggtgcaggt
     7201 ttgcgcatta atgaatgctt gcgtttgcgg gttaaagatt ttgattttga taatggctgc
     7261 atcactgtgc atgacggtaa gggtgggaaa agcagaaaca gcctactgcc cacgcgccta
     7321 atcccagcaa taaaataact cattgagcaa gcgcggctta ttcagcaaga cgacaactta
     7381 caaggcgtag ggccatcgct gccttttgct ttagatcaca aatacccttc tgcttatcga
     7441 caagcggcgt ggatgtttgt ctttccctcc agcacgctct gcaaccaccc gtataacggc
     7501 aaattatgcc gccatcatct gcatgactcc gttgcgcgaa aggcattgaa ggcagccgta
     7561 caaaaagcag gcatcgttag caagcgtgtc acttgtcata catttcgtca ctcgtttgct
     7621 acgcatctat tacaagcggg gcgtgatatt cgcactgtgc aagaactctt agggcataac
     7681 gatgttaaga ccacgcaaat ctatacgcat gtgttgggtc agcattttgc cggcaccacc
     7741 agtcctgcgg atggactgat gctacttatc aatcagtaaa agtagataaa tatctttatg
     7801 tagccctgag tagcccctga tactttttat ggtgaaaaag ataaacaatc atagagggga
     7861 taaatatgag ccagttctac cattgttcag ataagtttaa gcaagatact gtcaatcaca
     7921 gtgaccattc atggtcatta cacttcctcc agttgccaga caatgcctaa cataaaaacg
     7981 caaaagccca aaagtcagag cttaggtgtt ctaggcgttt tctaagcgct gaagaaatac
     8041 gtcttaccgt agattgcgtg cgtttctgaa gcacattttc cttatacatg gctttatccc
     8101 aggcagcttc atcaagctct tggagtaaca agccagtaat aatgcgacac tctcgggttt
     8161 gtagcgagcc gctggtgaga tcggagttat agcggaaagg ggtcatttgt aattatttgt
     8221 gggtggagga tagttaactt attattgtag ttggaagagg ctgctttcca aacattagca
     8281 cctcgtaatg cttgttactg tatattaatt tatgctctta gcgcatgtta atgggctatt
     8341 acaatgcata atgtccacat caagataact gtggcttaac acctgtacaa gcgaaaaaaa
     8401 tcaatcacct gtccggaaat acgggatcac tgtaaagtgt tggtgaggca gtctaggagg
     8461 gggatt
//
LOCUS       DAJTUN010000149         8453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTUN010000149 DAJTUN010000000
VERSION     DAJTUN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.149"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..223
                     /locus_tag="OO470_003469"
     CDS             <1..223
                     /locus_tag="OO470_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6252592.1"
                     /translation="AWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTH
                     SFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            475..1392
                     /gene="oxyR"
                     /locus_tag="OO470_003470"
     CDS             475..1392
                     /gene="oxyR"
                     /locus_tag="OO470_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6252593.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1375..2775)
                     /gene="sthA"
                     /locus_tag="OO470_003471"
     CDS             complement(1375..2775)
                     /gene="sthA"
                     /locus_tag="OO470_003471"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6252594.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3096..3746
                     /gene="fabR"
                     /locus_tag="OO470_003472"
     CDS             3096..3746
                     /gene="fabR"
                     /locus_tag="OO470_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6252595.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3746..4105
                     /locus_tag="OO470_003473"
     CDS             3746..4105
                     /locus_tag="OO470_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6252596.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4145..5233)
                     /gene="trmA"
                     /locus_tag="OO470_003474"
     CDS             complement(4145..5233)
                     /gene="trmA"
                     /locus_tag="OO470_003474"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6252597.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5347..5537
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5602..7446
                     /gene="btuB"
                     /locus_tag="OO470_003475"
     CDS             5602..7446
                     /gene="btuB"
                     /locus_tag="OO470_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6252598.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7391..8248
                     /gene="murI"
                     /locus_tag="OO470_003476"
     CDS             7391..8248
                     /gene="murI"
                     /locus_tag="OO470_003476"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6252599.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1963 a         2055 c         2248 g         2187 t
ORIGIN      
        1 tgcatgggga ctggtgcagg tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat
       61 tcaaaacttt ggcagttttg tttttgcttc ctttgctccg atcgtaaccg gttgggtagt
      121 ggataccaca cactcgttta atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg
      181 ggcgctgtgt tacttcttta ttgtcaaaga tcgcattgag taatcgttac tatcaggctc
      241 tcttgctgtg ggcctgtaga ataaaaaaaa gcggatcctg gagatccgca aaagttcacg
      301 ttggctttag ttattcgagt tgagaaactc tcgaaacggg cagtgacttc aagggttaaa
      361 agaggtgccg ctccgtttct gtgagcaatt atcagtcaga atgcttgata gggataatcg
      421 ttcattgcta ttctacctat cgccatgaac tatcgtggcg atggaggatg gataatgaat
      481 attcgtgatc ttgagtacct ggtggcattg gctgaacacc gccattttcg gcgtgcggca
      541 gattcctgcc acgttagcca gccgacgctt agcggccaaa ttcgtaagct ggaagatgag
      601 ctgggcgtga tgttgctgga gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg
      661 ctgctggtgg atcaggcgcg taccgtgctg cgtgaggtga aagtccttaa agagatggca
      721 agccagcagg gtgagacgat gtccggaccg ctgcatattg gtttgattcc tacggttgga
      781 ccatacctgc taccgcatat tatcccgatg ctgcaccaga catttccaaa gctggaaatg
      841 tatctgcatg aagcacagac ccaccagtta ctggcgcaac tggacagcgg caaactcgat
      901 tgcgtgatcc tcgcgctggt gaaagagagc gaagcattca ttgaagtgcc gttgtttgat
      961 gagccaatgt tgctggctat ctatgaagat cacccgtggg agaaccgcga atgcgtactg
     1021 atggccgatc tggcagggga aaaactgctg atgctggaag atggtcactg tttgcgcgat
     1081 caggcaatgg gtttctgctt tgaagccggg gcggatgaag atacacactt ccgcgcgacc
     1141 agcctggaaa ctctgcgcaa catggtggcg gcaggtagcg ggatcacttt attgccagcg
     1201 ctggctgtgc cgccggagcg caaacgcgat ggggttgttt atctgccatg cattaagccg
     1261 gaaccacgcc gcactattgg cctggtttat cgtcctggct caccgctgcg cagccgctat
     1321 gagcagctgg cagaggccat ccgcgcaaga atggatggcc atttcgataa agtgttaaaa
     1381 caggcggttt aaaccgttta acgcagctac ccgataggct tccgccatcg tcgggtagtt
     1441 aaaggtggtg ttgacgaagt actcaatagt gttgccgcca cctttctgtt ccataatcgc
     1501 ctgaccgata tgaataattt cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc
     1561 ttttgtttcc cgatggaaca aaattttcag cgtgcccacg ttcatgccga cgatttgtgc
     1621 acgtgccaga tgtttgaact gggcgcggcc cacttcatac ggcactttca tcgcggtcag
     1681 ctgctgttcg gttttgccca cagagctgat ttccgggatg gtgtaaatgc cggtagggat
     1741 atcttcaatc agatgtgcgg tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc
     1801 ctggtcatag gccgctgatg ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac
     1861 gtgcggctgt gcggtctgat acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc
     1921 cagcccaatg ttctgtaacg ccagcgaatc ggtattaccg gtacgaccgt tggcatagag
     1981 caggcagtca gctttcagtt ttttacccga cttcagatgc atgatcacac cgtcgtcaca
     2041 gctttcgatc ttctcgtact cttcgttgtg acgaatcact acgccactgt tccagaagtg
     2101 gtaggagaga gaatctgaca tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt
     2161 gatcagatcc acttttacat ccataccgcg gaagatcgac gcatattcac agccgatcac
     2221 tccagcacca tagataagta catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct
     2281 gtcgtaaatg cgtggatggg tgaaatcaac atctgttgga tgatatggac gagagccgca
     2341 ggcaataaca aatttttcag cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc
     2401 caacgtatgc tcgtcaacaa agcgagcgtt cccctgcaat atttcacagt gattacgttc
     2461 gtaaaatccc tgacgcatgc gcgtttgttg attaatcacg ttatcggcat ggttaaggat
     2521 atcggcaaaa gaagagcgga gcagtcggga atggtcgctg tatagtgggt tttgattgaa
     2581 ttctataatg cggctgacgg cgtgacggag agctttcgac gggatggtgc cccagtgggt
     2641 gcaaccgccg ccaacatttt gataacgctc gataactgcg acgcgcgcac cttgcttaac
     2701 caggcccatt gcagcgcctt cgccgccggg gccggaacct attactatgg catcgtaatc
     2761 gtaggaatgt ggcatggtag ggcttacctg ttcttataca taaaagcaac agaatggtaa
     2821 cattctatcg cgggtaagcc aattgatccc cgtcatttat ctggctatat cctgagcggc
     2881 ctttgctttg tctgtttctt acttttgccc tgacgtttta ttggattttt atcgacgata
     2941 ctctccgttt aagcggcagg tttccgctgt acgtaaaaga accggccagt aaatatgttt
     3001 tattgcgtta ccgttcattc acaatactgg agcaatccag tatgttcatt ctctggtata
     3061 gtgccagcag tacttttggc aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa
     3121 aagaaaaaac ccgccgttcg ctggtggaag ccgcatttag ccaattaagt gctgaacgca
     3181 gcttcgccag cctgagtttg cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt
     3241 tttatcggca tttccgcgac gtagacgaac tgggtctgac catggttgat gagagcggtt
     3301 taatgctacg ccaactcatg cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga
     3361 tccgtacctc ggtctccaca tttatggagt tcatcggtaa taatcctaac gccttccggt
     3421 tattattgcg ggaacgctcc ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa
     3481 ttcagcactt cattgcggaa cttgcggact atctggaact cgaaaaccat atgccgcgtg
     3541 cgtttactga agcgcaagcc gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg
     3601 cgttggacgt cggcgtcgaa caacgtcggc aattagaaga gcgactggta ctgcaactgc
     3661 gaatgatttc gaaaggggct tattactggt atcgccgtga acaagagaaa accgcaatta
     3721 ttccgggaaa tgtgaaggac gagtaatgaa acaagcaaat caagatagag gtacgctgct
     3781 gctggcgttg gttgctggtt tatcgattaa tggtactttc gcagcgctgt ttagctccat
     3841 tgtgccattt tctgtattcc cgattatttc cctggtgctg acggtttact gcctgcatca
     3901 acgttatctt aatcgcacca tgtcggtagg cttgccgggt ctggcagctg cctgttttat
     3961 tctcggcgta ctgctgtaca gcacggtagt tcgtgcggaa tatccggata tcggctctaa
     4021 cttcttcccg gcagtactct ccgtcattat ggtgttctgg attggcgcga agatgcgtaa
     4081 ccgtaagcag gaagttgctg agtaatcggt atcatgccgg atgctggcgc atccggcatg
     4141 ggttttactt cgcggtcagt aatacgccgc actccatatg gtgcgtgtag gggaactgat
     4201 caaacagagc cagacgttcg accttgtgcg tctggcttaa tgtttccaga tttttgcata
     4261 acgtttccgg attgcaggag atatacagaa tacgtggata cgcctgcacc attttctcgg
     4321 tttcactgtc cagaccgctg cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct
     4381 ttaagtcgat cccttgcagg cggttaaact cgcgcacacc attcatcgcc tgagtaaatt
     4441 cttctgccgc catacgaata atttgtacgt tatcaatatg gttagcggca atgttgtatt
     4501 gcgcggcagc gactgacggt ttggcgattt cggtggctaa tacccgatca aaattgcgcg
     4561 ccagcgctaa tgaaaagtta ccgttgccgc agtacagctc cagtaaatcg ccttttgagc
     4621 ctttggttac gtccagcgcc cattccagca tctgaatatt catcgccgcg ttcggctggg
     4681 taaagctgtt ttctacctga cgatagatca tctctttccc tgcgaccggc agacgctcgt
     4741 cgatgtaatc ttgatccagc gcgattttgg ttttcgttgc ccgaccaatc agatgcacat
     4801 tcagattctg cgcgcgcagt gcttccgctt cctgacgcca ctcatcatcc agctttttat
     4861 ggtacagcag ggaaaccacc gcctgattac tcagcgtggt gaggtaatca atctggaaca
     4921 acttgtggcg cagaacggga ttattacgca cacccgcaat catcgccgtc atcaactggt
     4981 tgatgagttc actggcggcg gggaagctat ccacgcggat gcggcttttg gtttgttgat
     5041 cgaaaatgat gtgatacagg tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc
     5101 ggtaatgact gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca
     5161 tactttgcaa acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt
     5221 gttcgggggt catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc
     5281 atcagatgtc cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt
     5341 tacaacctgt agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct
     5401 agagctgacg cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatc
     5461 cggtgggaag tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg
     5521 gccaacgtcg catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc
     5581 ttctattgtg gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc
     5641 acggcatttt ccgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctatc
     5701 cgttttgaac agccgcgcag cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat
     5761 atcgaccgct ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat
     5821 atcacccaaa acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc
     5881 agtcatgtgc tggtgttaat tgatggcgtc cgcctgaatc tggcgggggt gagtggttct
     5941 gccgacctta gccagttccc tattgcgctt gtccagcgta ttgaatatat ccgtggaccg
     6001 cgctccgctg tttatggttc cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc
     6061 gatgaacccg gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat
     6121 gtctctacgc agcaacaact gggggataaa acacgggtaa cgctgttggg cgattatgcc
     6181 catactcatg gttatgatgt tgttgcctat ggtaataccg gaacgcaagc gcagacagat
     6241 aacgatggtt ttttaagtaa aacgctttat ggggcgctgg agcataactt tactgatgcc
     6301 tggagcggct ttgttcgcgg ctatggctat gataaccgta ccaattatga cgcgtattat
     6361 tctcccggtt caccgttgct cgatacccgt aaactctata gccaaagttg ggacgccggg
     6421 ctgcgctata acggcgaact gattaaatca caactcatta ccagctatag ccatagcaaa
     6481 gattacaact acgatcccca ttatggtcgt tatgattcgt cggcgacgct cgatgagatg
     6541 aagcaataca ccgtccagtg ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg
     6601 ggtgtcgact ggcagaaaca gactacgacg ccgggtacag gttatgttga ggatggatat
     6661 gatcaacgta ataccggcat ctatctgacc gggctgcaac aagtcggcga ttttaccttt
     6721 gaaggcgcag cacgcagcga cgataactca cagtttggtc gtcatggaac ctggcaaacc
     6781 agcgccggtt gggaattcat cgaaggttat cgcttcattg cttcctacgg gacatcttat
     6841 aaggcaccaa atctggggca actgtatggc ttctacggaa atccgaatct ggacccggag
     6901 aaaagcaaac agtgggaagg cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt
     6961 tccggatatc gtaacgatgt cagtgacttg atcgattatg atgatcacac cctgaaatat
     7021 tacaacgaag ggaaagcgcg gattaagggc gtcgaggcga cagccaattt tgataccgga
     7081 ccactgacgc atactgtgag ttatgattat gtcgatgcgc gcaatgcgat taccgacacg
     7141 ccgttgttac gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac
     7201 ttcgactggg gtattactta tcagtattta ggcactcgct atgataagga ttactcatct
     7261 tatccttatc aaaccgttaa aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat
     7321 ccggtcacct ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattat
     7381 gagacagtct atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc
     7441 ttctgaacca cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta
     7501 tgacgagatc cggcatctct taccggatct ccattacatt tatgctttcg ataacgtcgc
     7561 tttcccgtat ggcgaaaaaa gcgaagcgtt tattgttgag cgagtggtgg caattgtcac
     7621 cgcggtgcaa gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac
     7681 cgtttcactt cctgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc
     7741 gattaaacct gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg
     7801 aacagttaaa cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat
     7861 agaaatgctg ggctcggcag agatggttga gttggctgaa gcgaagctgc atggcgaaga
     7921 tgtttctctg gatgcactaa aacgtatcct acgcccgtgg ttaagaatga aagagccgcc
     7981 agataccgtt gtattgggtt gcacccattt ccctctacta caagaagaac tgttacaagt
     8041 gctgccagag ggaacccggc tggtggattc tggcgcagcg attgctcgcc gaacggcctg
     8101 gttgttagaa catgaagccc cggatgcaaa atctgccgat gcgaatattg ccttttgtat
     8161 ggcaatgacg ccagaagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac
     8221 gctcgaaaaa ctggcagttt taggctgatt tggttgaatg ttgcgcggtc ggaaaattat
     8281 tttaaatttc ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga
     8341 acaacggcaa acacgccgac gggtcagcgg ggttctcctg agaattccga cagagaaagc
     8401 aaaaataaat gcttgactct gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DAJTUN010000150         8336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTUN010000150 DAJTUN010000000
VERSION     DAJTUN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.150"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(456..1652)
                     /gene="hemY"
                     /locus_tag="OO470_003477"
     CDS             complement(456..1652)
                     /gene="hemY"
                     /locus_tag="OO470_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6252600.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1655..2872)
                     /gene="hemX"
                     /locus_tag="OO470_003478"
     CDS             complement(1655..2872)
                     /gene="hemX"
                     /locus_tag="OO470_003478"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6252601.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(2894..3634)
                     /gene="hemD"
                     /locus_tag="OO470_003479"
     CDS             complement(2894..3634)
                     /gene="hemD"
                     /locus_tag="OO470_003479"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6252602.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3631..4572)
                     /gene="hemC"
                     /locus_tag="OO470_003480"
     CDS             complement(3631..4572)
                     /gene="hemC"
                     /locus_tag="OO470_003480"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6252603.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4959..7505
                     /gene="cyaA"
                     /locus_tag="OO470_003481"
     CDS             4959..7505
                     /gene="cyaA"
                     /locus_tag="OO470_003481"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6252604.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7545..7865)
                     /gene="cyaY"
                     /locus_tag="OO470_003482"
     CDS             complement(7545..7865)
                     /gene="cyaY"
                     /locus_tag="OO470_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6252605.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7913..>8336)
                     /locus_tag="OO470_003483"
     CDS             complement(7913..>8336)
                     /locus_tag="OO470_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6252606.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1866 a         2182 c         2154 g         2134 t
ORIGIN      
        1 gtttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt ttatcattca
       61 taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt tattactcag
      121 gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt atttcccgat
      181 tctctgtggc ataataaacg agtagatgct cattccatct cttatgttcg ccttagtgcc
      241 tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact gacagatgtc
      301 gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac ttgttgtcat
      361 acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat gcatcagaaa
      421 ggtcccggag gtgctttcct ccgggtgaga aggaactact gtggcgggtt attctgcaac
      481 gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc
      541 agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg
      601 aaggcgagcg atgcttcctg ccattcaccg tgcttcatca gtgactgacc caatgtgctc
      661 cacaacagcg ggcgatcacc gacgtttttg atttgctggc gtagcacttt ttccagctgt
      721 tccgggttat tggttttcag acgcgggatc ggcagcagta ggcgatcgtc atactggcgt
      781 ttcaggccgt cgatgataat ttgctgggca gtatcatgat cgtcacattc aataagatgt
      841 tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg gtttttccac
      901 cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag gccaatccat
      961 gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt cgccattgat
     1021 gggatgatat ccagcagaga actccatgcc cctgtgcgga tatacgcctg ttccgccaga
     1081 cgtaatactt caggatggcg tggcgtaact tccagcagct tatccacgcc gtggcgtgca
     1141 gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac cggaatggtg
     1201 tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca
     1261 cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc agcgtgatcg
     1321 gcatttttcg ccatcagctt ttcaacttgc tgataatcac cttccgccag tttcagcagc
     1381 gcctgttcgg tctgcttacg cgcacggcga cgcttacgtc cgacaaacca tccacgggtg
     1441 tgcgcgccag tgcggaagat ccgccgcagt agccactcaa tggcaaacag cactaccatc
     1501 gccagaatca atatgatcgc caggcccgtg acgctggttt cgatattgta gttgtcggtc
     1561 tggatcagca cataaccctg atgaccggca atcatcgggc caaccactat ccccgcaatc
     1621 agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc gcggctgccg
     1681 gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg
     1741 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
     1801 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     1861 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     1921 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     1981 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2041 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2101 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2161 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     2221 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     2281 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     2341 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     2401 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     2461 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     2521 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     2581 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     2641 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     2701 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     2761 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     2821 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     2881 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     2941 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3001 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3061 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     3121 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3181 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     3241 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3301 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     3361 cggtggtgcg tccaatggcg aaataagtag gtagttgggg ccatttacga tcttgctgat
     3421 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     3481 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     3541 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc
     3601 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     3661 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     3721 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     3781 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     3841 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     3901 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     3961 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     4021 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4081 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     4141 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4201 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     4261 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4321 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     4381 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     4441 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     4501 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     4561 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     4621 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     4681 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     4741 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     4801 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     4861 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     4921 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     4981 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5041 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5101 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5161 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     5221 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5281 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5341 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     5401 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     5461 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     5521 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     5581 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     5641 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     5701 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     5761 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     5821 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     5881 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     5941 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     6001 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     6061 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     6121 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6181 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6241 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     6301 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     6361 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     6421 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     6481 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     6541 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     6601 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     6661 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     6721 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     6781 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     6841 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     6901 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     6961 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7021 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     7081 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     7141 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     7201 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7261 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     7321 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     7381 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     7441 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     7501 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     7561 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     7621 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     7681 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     7741 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     7801 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     7861 ttcattgttg tatcctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     7921 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     7981 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     8041 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     8101 cgaagcgatc gtttttagtt agcgaaccgt taactggaat aatttgcaag ctctttgacc
     8161 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     8221 ggtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     8281 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgt
//
LOCUS       DAJTUN010000151         7853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTUN010000151 DAJTUN010000000
VERSION     DAJTUN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.151"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..847
                     /locus_tag="OO470_003484"
     CDS             <1..847
                     /locus_tag="OO470_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6252607.1"
                     /translation="DAERLMFSRELNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPE
                     EAYRNGTIQRQIAGFDEILRSPKLPAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKEN
                     VDNRVATVTVSSTTGFKRGDKISFIGVKFLSQMAKNVLTDDATFSITRVIDGTHIEIT
                     PKPIALDDASLTKEEKAYANVNTSLADTTPVNVLNVATTTANVFWADDSIRLLSQPIP
                     VTHELFAGMKTSSFSIPGIGVNGIFATQGDINTLSGKCRIAVWYSACAVRPEAIGVGL
                     PNQTA"
     gene            892..1116
                     /locus_tag="OO470_003485"
     CDS             892..1116
                     /locus_tag="OO470_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252608.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEEKQCKKPGRKPKAAADEPDNEG"
     gene            1094..1594
                     /locus_tag="OO470_003486"
     CDS             1094..1594
                     /locus_tag="OO470_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6252609.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1594..3012
                     /locus_tag="OO470_003487"
     CDS             1594..3012
                     /locus_tag="OO470_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6252610.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            3012..3809
                     /locus_tag="OO470_003488"
     CDS             3012..3809
                     /locus_tag="OO470_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252611.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVDTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATTIKIIAEQ"
     gene            3809..4264
                     /locus_tag="OO470_003489"
     CDS             3809..4264
                     /locus_tag="OO470_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6252612.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            4267..4875
                     /locus_tag="OO470_003490"
     CDS             4267..4875
                     /locus_tag="OO470_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6252613.1"
                     /translation="MLYAFKLGRKLCGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALASLLGTSTPWGAGIGAGIGLLGSLF"
     gene            4885..6216
                     /locus_tag="OO470_003491"
     CDS             4885..6216
                     /locus_tag="OO470_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6252614.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            6217..>7853
                     /locus_tag="OO470_003492"
     CDS             6217..>7853
                     /locus_tag="OO470_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6252615.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDA"
BASE COUNT         2073 a         1931 c         2051 g         1798 t
ORIGIN      
        1 tgatgcagag cgactgatgt tctcccgtga gctaaaccgc gacatgggca tcagttactt
       61 cctgaaccct gacgattacc gcaaagcagg ccgcaacctg gtagatggtg acatcttcgg
      121 gcgcgttcct gaagaagcat atcgaaacgg tactattcag cgtcagattg ctggctttga
      181 tgaaattctt cgctcaccga aacttccggc agttaccaag tcaaccgcga ctggtgtaac
      241 tgtttctggt gcgcagaagt ttaagccgca ggcatacacc cttgataccg atggtaacaa
      301 agagaacgtc gacaaccgtg ttgcaacggt gaccgtatcc tccaccaccg gatttaagcg
      361 cggcgacaaa atcagcttca ttggtgtgaa gttcctgtct cagatggcga agaatgtgct
      421 gactgatgac gcgactttct caatcacccg tgtgatcgat ggtactcaca tcgaaatcac
      481 gccgaagccg attgcgctgg atgacgcgtc actgacaaaa gaagagaagg cttacgctaa
      541 cgtaaacacc tctcttgctg ataccactcc ggtaaacgtt ctgaacgtgg caacaaccac
      601 cgctaacgtg ttctgggctg atgactcaat ccgcctgctg tctcagccga tcccggtaac
      661 ccatgaactg tttgctggca tgaaaacgtc ttccttcagc attccaggta ttggtgttaa
      721 cggcatcttc gcaacgcagg gtgatatcaa cactctgtct ggtaagtgcc gtattgctgt
      781 gtggtattca gcatgtgctg tacgaccaga ggcaattggt gttggtctgc ctaaccagac
      841 cgcgtgataa ccagagggag cttcggctcc cgtttttatc tggagacaag catgacacac
      901 atgatctttc gtcatggcga catgaagaag tggaaaggcg ttggctacga ctttgaaatc
      961 gtgaaagccg aagagcttca ggaatatctg gatgctggct ggttttcaca tcctgatgac
     1021 cttttgaagg atgttgcaga gccagaagaa aaacagtgta aaaagcctgg tcgaaaacct
     1081 aaggcggcag cagatgaacc tgacaacgaa gggtgattta gttcttgcgg cattacgtaa
     1141 gctcggtgtt gcatcaaatg ccacgttaac cgatgtcgaa ccgcagtcta tggaagacgg
     1201 cgtcaacgac cttgaaatga tgatggctga atggcttggc ggtgatgcgt cacctggtat
     1261 caacgttggc tacatttttg ctgatgcaga tgttgctccg gatccgggcg atgagcacgg
     1321 tttatcaaat aacgctatca atgccgtcat tttcaacctt gcctgccgaa ttgctccaga
     1381 ttatgcgctg gaagcgtctg caaaacttat aaccactgcc agatacggga aagagcgact
     1441 cgtcaaactg tctgcaatgg acagggcaaa agcagctaaa tgtaagtccg gttatccaaa
     1501 ccgtatgcct gttggtagtg gtaaccagtt ggcgaagtgg aatggttgga attacttcca
     1561 ccgaaaggaa ccttgcgata acgggagcga ataatgccta ttcagcaact tccgcttatg
     1621 aaaggtgtcg gcaaagactt ccgaaacgcc gactatatcg actatctgcc agtgaatatg
     1681 ctggctacac ccaaagaaat cctgaacagc agcggatatc ttcgctcatt cccgggcatt
     1741 gccaaacgtt ctgacgtgaa cggtgtatct cgcggcgtcg agtacaacat ggcgcagaat
     1801 gctgtttatc gcgtgtgtgg cggcaagctg tacaaaggag aaagtgaagt cggtgatgtt
     1861 gccggaagtg gtcgtgtatc aatggcgcat ggtcggacat cacaggcggt aggcgttaat
     1921 ggtcaactgg tcgagtatcg ctatgatggc acggtcaaaa ccgtctcaaa ctggcctaca
     1981 gacagtggat tcactcagta tgagttaggt tcagttcgcg acattacgcg cttacgtggg
     2041 cgttatgcgt ggtcaaaaga cggaactgat tcatggttta tcactgacct tgaagacgaa
     2101 tctcatcctg accgttacag cgcacaatat cgtgccgagt ctcagcctga cggcatcatc
     2161 ggtatcggga catggcgaga cttcatcgtc tgctttggtt catcgactat tgaatatttc
     2221 tccctgactg gcgcaaccac cgttggtgcc gctttgtatg ttgcacaacc atcgctgatg
     2281 gtgcagaaag gcattgccgg aacttactgc aaaacgccat tcgctgattc gtatgcgttt
     2341 atcagcaatc cggcaacggg tgcgccgtct gtgtatatca tcgactccgg tcaggtgtca
     2401 ccaattgcca gcgcgagcat tgagaaaatc ctccgctcct acactgctga tgaactggct
     2461 gatggtgtga tggaatcgct gcgatttgat gctcatgagt tgctgattat ccaccttccg
     2521 cgtcacgtcc tcgtgtacga cgcatcttca agcgccaatg gcccacaatg gtgtgtgctg
     2581 aaaacaggtc tgtatgacga tgtgtaccgc gctatcgact tcgtttacga aggcaatcag
     2641 ataacgtgcg gcgataagct ggaatctgtt accgggaaac tgcaattcga tatcagcagc
     2701 cagtacgaca agcaacagga acacctgctg tttactccac tcttcaaagc ggataacgcc
     2761 agagtgttcg accttgaggt tgaatcgtca actggcgttg cgcagtacgc cgaccgcctt
     2821 tttctctctg caaccactga ctgcatcaat tacgggcgtg agcagatgat tgagcagaat
     2881 gaaccgttcg tttacgacaa gcgtgttttg tggaagcgag tagggcgcat caggaaaaat
     2941 gtcggcttca aattgcgcgt tatcacgaag tcacctgtca ctctgtctgg cgctcagata
     3001 aggattgagt aatggctgat tcgaatctca atgtgccggt aatcatccaa gctacgcggc
     3061 tcgatacatc agtccttcca cgcaatatct tctcgcagtc gtatctgctt tacgttatcg
     3121 cacagggcac tgatgttggt aacgtggcta acaaagccaa cgaggccgga cagggcgctt
     3181 atgatgcaca ggtcaggaac gatgagcagg atgtgattct cgctgaccat gagcagcgaa
     3241 tttctgctgc ggaagcaacg cttgttaatc atgaggagcg aatcagacag gcagaatcaa
     3301 ctcttcagga ccatgaaaca cgaatagctc agaatgaaag cgatatttcg tcgcttgaca
     3361 caagagttca gtcgctggag tcgcaggttt cagaccatga aacgcgcatc gatgctctgg
     3421 agtatgcaac cacacgcaag aagtcagagg ttgtttactc tggtgtatct gtaaccatcc
     3481 cgacagcgcc gaccaacctt gttagcctgc tgaaaacgct cacaccgtca tccggctcgt
     3541 tggcaccatt cttcgacacc gttaacaaca agatggttgt gttcaacgag aacaaaaccc
     3601 tgctcttcaa gttgtcgatt gtcgacacgt ggcccagcgg aaccgccaac aggtcaatgc
     3661 agctaacctt ttccggctct gttcctgaca cactggtcag cagtcgtaat gcggcgacaa
     3721 caaccgacaa catcctgtta gctacgttct tcagcgtgga taaagacggc tttcttgcca
     3781 caaccatcaa gataatcgcg gagcagtgat gattacattc aaaccaacgc gaaacatcga
     3841 cctgatagaa gcagtcggaa atcaccctga cattatcgcc gggagcaaca acggtgatgg
     3901 atacgactac aaacctgaat gccgttactt tgaggtgaac gtgcacggtc agtttggcgg
     3961 cattgtttac tatcaggaga ttcagccgct gacattagat tgccacgcca tgtacctgcc
     4021 agagattcgc ggcttcagca aggaaattgg gctggcgttc tggcgataca ttctgactaa
     4081 caccaccgtt cagtgcgtaa catcgttcgc tgcacgcaaa ttccgacacg gtcagatgta
     4141 ctgcgcaatg attggcctta agcgtgtagg aaccatcaag aaatacttca aaggcgtgga
     4201 tgacgtgact ttttacagcg ccacacgcga agaactaatc gacttcctga atcacgggag
     4261 atagccatgt tatatgcatt taagctgggc agaaaactgt gcggcgagga accttattgc
     4321 cctgaaaaag gcgggaaagg tggcagttct gataaaagcg caaagtatgc agcagaagcc
     4381 cagaagtatg ctgcagacct gcaaaatcag cagtggcaga cgatcatgaa aaaccttgct
     4441 ccgttcacgc ctcttgcgga gcagtatgtt aaccagcttc agaacctttc cagtttagaa
     4501 ggtcaggggc aggcacttaa tcagtattac aactctaagc agtataaaga ccttgcaggg
     4561 caggcgcgtt accagagtct tgctgctgcg gaggcgacgg gtggacttgg ttcgacagcc
     4621 acaagcaatc aactggctac gatcgcgccg acactcggtc agtcttggtt atcaaatcag
     4681 atgagcaatt acaacaatct ggcaaacgtt gggcttggtg cgctgcaagg tcaggcaaac
     4741 gccgggcaga cgtacgccaa caacatgagc agcattgcac agcaaagcgc agctcttgcc
     4801 agccttttgg gaacatcaac gccttggggc gctggtattg gtgctggtat cggattgctt
     4861 ggctcgttgt tttaaggggt aatcatggct acttggcaag gaacaaacgg cggattgttg
     4921 gctggtatcg gcggcgtcaa ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt
     4981 cagcttatca ggcagaacaa tgatattgag cgttcaggcg ctaacaatgt tgggctgact
     5041 gctttgcaag gtctttcagg tattgcgggt gtttttcagc aggaaaagca ggctcagcgg
     5101 cagaaagaat ttcagcaggc gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg
     5161 cgtcagttgg ctactcaata tccagaccag attgaatccg ttcgtaaagg catgggattc
     5221 attgatgaag agcagcgtaa ttctatcggc accttagcgg ctggcgcacg ccttgcgtca
     5281 tcgtctccag aagcaatgca atcatggctg caaaacaacg ccggtgagtt agctcgtgtt
     5341 ggcgttaatc ctcatgacgt cgctcagatg taccaacaga acccgcggca gttcggcgaa
     5401 tttgtcgatc acctggggat gaacagtctc gggcccgaaa aatactttga cctacaggat
     5461 aaaatgcagg gtcgccaggt taccatgcgc ggtcaggatc tggattcgca aaccgccgct
     5521 cggaatcagg caatcacaat gcgcggacaa gatatccagg cgaatttagg tcagcagcgc
     5581 attaatctgg acgcagaaac aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg
     5641 ctatcagcag aaactaacga cctgaagcgc caggaaatac agagccgcat agcagccaac
     5701 aaccagcagt tgcagcagaa gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac
     5761 accctcacaa ccagcatgtt cactctgaac gatatcgtta gttctccttc acttaagagc
     5821 attacaggct tacgtggagt aatccccaac gttccaggct cacaggctgc agacactcag
     5881 gcacgacttg atacctttaa atcacaagca tacctgacag cggttcaggc catgcgaggc
     5941 atgggcgcac tttctgatgc cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag
     6001 aactcgcaga gcgaggagtc ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac
     6061 cagaagcgta atgaggcggt tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa
     6121 gcgcctcagg cttctataga ttacctgaag cagcaccccg agctgtcaat cgacttcatt
     6181 aatcgctacg gatatcttcc atctttgggg cagtaaatgg ctaattaccg tgatttgtta
     6241 gagcaggctg gcgcacgtta cggtgtgcca gaagggttga tgactgcact gggtgccaag
     6301 gagtcttctt acaaccctgc cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc
     6361 atgcctggga catggcgtga tatgggttat accgatgagc aaatgcaaaa ccccgaatat
     6421 caggctgacg ctggcgcgcg ctatctggca aagatgtacc agcagtttgg taactggcgt
     6481 gacgctcttc aggcttatca cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa
     6541 tatacgccag gacctgaagg ccgcggttac gttgatgatc gctttgctca atgggctggt
     6601 gacccggtga cagactcaac agtcgaacag cgcgccacct ctgcaaaggt acatcctcag
     6661 caagacccta acaacccgtt tgcacaactg gaagtacagt catccgaaca agtatcggca
     6721 tcaggtgtgc agtcagaccc aaataatcca tttgctcaga ttgagcagca ggcagccagt
     6781 cagcagccac ctcaacccgt aagttctgtc gcaccgaaac ctgttcagca acaaacagtt
     6841 aatcaggcca ataatgcacc aacacgggaa gaaccatcat tgatgcaaca agctggcgat
     6901 tggctcactg gtggtcaaag tgcagggcaa attgcagaac aggctggtcg tggtctggta
     6961 aacataccat ttgacgtatt gcagggtggc gcaagtctga ttaatgcaat cagtcagggg
     7021 cttggggggc caaaagtatt ggatgatgtt tatcgtccag tagacagacc gacagacccc
     7081 tacgcgcaag ctggagagtc aataggcggt tatcttgttc ctggagcagg tgtagctgga
     7141 aacatggtca ttggttctct cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat
     7201 gccgctatta atgccggact tagcattgct acgcatggcc tgataaatgg cgttacccgt
     7261 ggtgttcgtg gtgcatcaaa tataattagt ggcaataaaa catctgcaca gagagcgacc
     7321 actgcgccaa cagaaacatc accattctcc ggtgatgccg ctgcagcaac aaatcctgcg
     7381 gttcatgccg cagaggcaag agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac
     7441 ccagaggaag tcgttcgcac agtagcagca caaaaaaggc caaatctcgc ttcatcgctt
     7501 gatgaactag atatcaatcc tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt
     7561 gattcattac tcccttcaca cttttccggg aacgagcaat acaaggcagt tgagcaagca
     7621 atcaagtccc gtgcgggttc tgctctacag gtgcaggaaa atgaagcaat caggcagcta
     7681 gcacagggcg cgggtgagat aattgatcgc gtttccggtg caaaagatgc tcttggtatg
     7741 agcgacaagt ttattgatac ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac
     7801 cagctttatc gcaatgttga aaaggcgatg cctgcaggtg caaaaattga tgc
//
LOCUS       DAJTUN010000152         7674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTUN010000152 DAJTUN010000000
VERSION     DAJTUN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.152"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..477
                     /gene="ymdB"
                     /locus_tag="OO470_003493"
     CDS             <1..477
                     /gene="ymdB"
                     /locus_tag="OO470_003493"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6252616.1"
                     /translation="IVNAANPSLMGGGGVDGAIHRAAGPALLDACLKVRQQQGDCPTG
                     HAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYLNSLRLVAANSYTSVAFPAI
                     STGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYDEENAHLYERLLTQQGDE"
     gene            419..1900
                     /gene="clsC"
                     /locus_tag="OO470_003494"
     CDS             419..1900
                     /gene="clsC"
                     /locus_tag="OO470_003494"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6252617.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(1908..3065)
                     /gene="mdoC"
                     /locus_tag="OO470_003495"
     CDS             complement(1908..3065)
                     /gene="mdoC"
                     /locus_tag="OO470_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6252618.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3459..4994
                     /gene="mdoG"
                     /locus_tag="OO470_003496"
     CDS             3459..4994
                     /gene="mdoG"
                     /locus_tag="OO470_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6252619.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4987..7530
                     /gene="mdoH"
                     /locus_tag="OO470_003497"
     CDS             4987..7530
                     /gene="mdoH"
                     /locus_tag="OO470_003497"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6252620.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1891 a         1846 c         2030 g         1907 t
ORIGIN      
        1 attgtgaatg cggctaatcc gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat
       61 cgcgcagcgg gtccggccct gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat
      121 tgccctacgg ggcatgccgt tattacgctt gcaggcgatc ttcccgctaa agccgtagta
      181 cacaccgtcg ggccagtctg gcgtggtggg gaacaaaacg aagaccagct tttgcaggat
      241 gcctatctga atagcctacg actggtggcg gcaaacagct atacgtcagt ggcttttcct
      301 gcaatcagta ctggggttta tagttaccct cgtgcggcag cggctgaaat cgcagttaaa
      361 accgtttcag aatttattac ccgtcacgct ttacccgaac aggtatactt tgtctgttat
      421 gatgaagaaa acgcccacct ctacgaaaga ctccttaccc aacaaggaga tgaatgattt
      481 gccccggctg gcgagcgcgg tgctgccact gtgttcgcaa catcccggtc agtgtggtct
      541 tttccctctg gagaaaagtc tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc
      601 agagcatacg ctggatgttc agtattacat ctggcaggac gatatgtcgg ggcggttact
      661 gttttccgcc ctgttagccg cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga
      721 caacaatacg cccggacttg acgacatttt gcgcttgctt gacagtcatc cacgcattga
      781 agtccggctt tttaatcctt tctcgtttcg cttgctacgt ccgcttggat atatcaccga
      841 cttttcccgt ctcaatcgcc ggatgcacaa taaaagtttc actgtcgatg gcgtggttac
      901 cctagtggga ggacgaaata ttggtgatgc ctattttggt gcaggggaag agccactttt
      961 ttcggattta gatgtcatgg caataggccc cgtggtagag gatgttgccg atgatttcgc
     1021 ccgctactgg tattgcaaat cggtttcacc cttacagcag gtgctggatg tcccggaagg
     1081 tgaaatggcg gatcgcatcg agttacccgc ctcctggcat aacgatgcca tgacgcatcg
     1141 ttatttacgc aaaatggaat ccagtccatt tataaatcat ctggttgatg gaacgttgcc
     1201 gcttatctgg gcgaagacac gtttattaag tgatgatccg gcgaaagggg agggcaaggc
     1261 aaaacggcat tcactgttac cgcagcgcct gttcgatatc atgggctcac ccagtgaacg
     1321 catcgatatt atctcttcct attttgtacc gacacgcgca ggtgtggcgc aactcttacg
     1381 gatggtgaga aaaggggtaa agattgcgat cctaaccaat tctcttgccg ctaacgatgt
     1441 tgctgtcgtc catgccggat acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga
     1501 attatatgaa ctcaagccga cgcgtgaaca aagcagtacg ttacacgatc gcggcataac
     1561 cggtaattcc ggtgccagcc tgcatgctaa aacttttagc atcgatggta aaacggtgtt
     1621 tatcggctct ttcaatttcg atccgcgttc aacattgctc aatactgaaa tgggcttcgt
     1681 gatagagagc gaaacgctgg cacagttaat tgataaacgc tttattcaga gccagtacga
     1741 tgcggcctgg cagctccgtc tggacaggtg gggacggatc aactgggttg atcgtcatgc
     1801 aaagaaagag gttattctca aaaaagaacc cgccaccagt ttctggaagc gggttatggt
     1861 cagactggcg tcgatattgc ccgtggaatg gttattgtaa atgtggctta acgggctggt
     1921 gctttatcgt tctcacgctt aacaaccggt ttaccagaga acaaaaactt cagtaacggg
     1981 atgcgtaaat gaatttcata cagaattatc gcaatcccta ctacgaatat caggccacag
     2041 agaaaaccaa gccagttgga ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt
     2101 aacgggtggt gaaccagata gataaacagc gatgcgttaa caaaataagt cacccgcgct
     2161 gactggaagt taagcaaacg gtggccaaag gagaagacca cattcaccat ccacaggccg
     2221 aggaccatgg tgatcaccga ctcggtttcg tacatccagg catcgccact gccatagcgc
     2281 tggttgagta aataagcgac aaacgccaat gctgcagcga gggtacagcc acgagacggc
     2341 gtggtaaaca aggctttaag atgagggaaa atgaaagcca gtgcgccgag gataaagaac
     2401 ggtaaataaa atagcgtttg catgacaata aaattgaaca tgccattact cagaatgggt
     2461 ggatacacaa taaaaatcgt tcttcttatt accgcatagc cgatgccgag gcataaaaaa
     2521 atcaccgata gttttaccat cgagaatttt ttattcgttt tatcagaatt ttctaaatta
     2581 tttctgatgc gcttaaatat ccatacacac agcgtcgtca tgactactaa caccagtaaa
     2641 aaccacaggt gtgatattaa ttcccaggcc aacgtattat atttgtcata caatgatagc
     2701 ccaggccaac tttccgcttt ccctttgaca tattgcagca taataaattg cggtaatgtc
     2761 agtaggggga tggctgttaa catcgggata cctacacgtt cgacacgtac tttccaccat
     2821 tttttcaagg gatagcgtaa aaaaagcatg taggaaaagt atccggatat aacgaaaaat
     2881 acctgcatgc ggaacgagtg gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg
     2941 gcgctattca catgccatgt atggctcgaa tagattaaag aaatatgaaa agggatccct
     3001 aacaacatca gccaggcgcg gatggagtcg aggaaatatt cacgttgcgc gggtactgga
     3061 ttcatatatg gttaactaat ctcggatttt tcgtcttatc cctgtcgggt tatgccttta
     3121 ggcttgttgc catagcgaca ccgacctgac cgcgccaggc gcaggcttca aggtttttat
     3181 gcatagcatc atcgctacca ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata
     3241 aatttgctgg caaaccctac acgaagtcga tgcttctgtc tttaggagaa gcacggaaag
     3301 tgaaaacggt tgcaatcagg tgcttaatcc atgagccagc gtgctgaacg ataccgggat
     3361 tcagttgtcg gaatggctgg ttatccatta aaatagatcg gatcgatata agcacacaaa
     3421 gggggaagtg cttactaatt atgaaacata aactacaaat gatgaaaatg cgttggttga
     3481 gtgctgcagt aatgttaacc ctgtatacat cttcaagctg ggctttcagt attgatgatg
     3541 tcgcaaagca agctcaatcc ttagccggga aaggctatga ggcgcctaaa agcaacttgc
     3601 cctccgtttt ccgcgacatg aaatacgcgg actatcagca gatccagttt aatcatgaca
     3661 aagcgtactg gaacaatctg aagaccccat tcaaactcga gttctaccat cagggtatgt
     3721 acttcgatac cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat
     3781 acagcccgga ttatttcact ttcggcgatg ttcagcatga caaagacacg gtaaaagacc
     3841 ttggttttgc cggttttaaa gtgctttacc cgatcaacag caaagataaa aacgatgaaa
     3901 tcgtcagcat gctcggggcc agctatttcc gcgtgattgg tgcaggtcag gtttatggcc
     3961 tttctgcccg cggcctagca attgataccg ctttgccatc gggtgaagaa tttccacgct
     4021 tcaaagagtt ctggatcgag cgtccaaaac cgactgataa acgtttaacc atttatgcat
     4081 tgcttgactc gccgcgtgcg acaggtgctt acaaattcgt ggttatgcca gggcgtgaca
     4141 cggttgtgga tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg
     4201 caccgttaac cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca aataactatc
     4261 gtccggagtt gcacgactct aacggtctgt ctatccatgc tggtaatggc gaatggatct
     4321 ggcgtccgtt gaataacccg aaacatttag cggtcagcag cttctccatg gaaaacccgc
     4381 gaggctttgg tctgttgcaa cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc
     4441 gttacgatct ccgtccaagc gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg
     4501 ttgagctggt ggaaattcca accaacgatg aaaccaacga taacatcgtc gcttactgga
     4561 cgccggatca gctgccggag ccgggtaaag agatgaactt taaatacacc atcaccttca
     4621 gccgtgatga agacaaactg catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt
     4681 caacggggga tgtgaagcag tcgaacctga ttcgccagcc tgacggtact atcgcctttg
     4741 tggtcgattt taccggcgca gagatgaaaa aactgccaga ggataccccg gtcacagcgc
     4801 aaaccagcat tggtgataat ggtgagatag ttgaaagcac ggtgcgctat aacccggtta
     4861 ccaaaggctg gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa
     4921 tgcgtgctgc gctggtgaat gccgatcaga cgttgagtga aacctggagc taccagttac
     4981 ctgccaatga ataagacaac tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa
     5041 gcagcattgc cgaagactga tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc
     5101 acctgggcgc gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc
     5161 tggccagatt cacttgctga tggacagtta attaaagacg acgaagggcg cgatcagctt
     5221 aaggcgatgc cagaagcaaa acgctcctcg atgtttcccg acccgtggcg taccaacccg
     5281 gtaggccgtt tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt
     5341 ttgaccaaag aagagcagga gagcgagcaa aagtggcgta ccgttggtac catccgccgt
     5401 tacattctgt tgatcctgac gctcgcgcaa actgttgtcg cgacctggta tatgaagacc
     5461 attcttcctt atcagggttg ggcgctgatt aatcctatgg atatggttgg tcaggatttg
     5521 tgggtttcct ttatgcagct tctgccttat atgctgcaaa ccggtatcct gatccttttt
     5581 gcggtactgt tctgttgggt gtccgccgga ttctggacgg cgttgatggg cttcctgcaa
     5641 ctgcttattg gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta
     5701 aacccggagc atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt
     5761 tttgctggct tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacacttt
     5821 gatgtctaca ttctgagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc
     5881 tggatggagc ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt
     5941 cgccgcgtga agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag
     6001 tacagctaca tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttatgcggc
     6061 ctggtgcgct tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg
     6121 tccggtatgg atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca
     6181 ctgtttacag ccggtttgca cttctggcaa cttggcgagt cgcactactg gggacataac
     6241 gcgattatcc gcgtgaaacc atttatcgag cactgcgcac tggctccgct gccgggcgaa
     6301 ggttcctttg ccggttcaat cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt
     6361 gcaggttggg gggtctggat tgcttacgat ctcccgggtt cttatgaaga attgccgcct
     6421 aacttgcttg atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc
     6481 cgtctgttcc tggttaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg
     6541 atgtcttatc tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag
     6601 gtagtgcatg cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggtg
     6661 tggccgcagt ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg
     6721 ttcctgccga agttattgag cattttgctt atctggtgca aaggaacgaa agaatacggc
     6781 ggcttctggc gcgttacatt atcgttgctg ctggaagtgc tgttttccgt gctgctggct
     6841 ccggtacgca tgctgttcca tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg
     6901 gtgtggaatt caccgcagcg tgatgatgac tccacttcat ggggcgaagc gttcaaacgc
     6961 cacggctcac agctgctgtt ggggttagtg tgggctgttg ggatggcgtg gctggatctg
     7021 cgtttcctgt tctggctggc accgattgtc ttctcgttga tcctgtcacc gtttgtttcg
     7081 gtgatttcca gccgtgccac cgttggtctg cgcaccaaac gctggaaact gttcctgatc
     7141 ccggaagagt attcaccgcc gcaggtgctg gttgataccg atcggttcct tgagatgaat
     7201 cgccaacgct cccttgacga tggctttatg cacgcagtgt ttaacccgtc atttaacgct
     7261 ctggcaaccg caatggcgac agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt
     7321 gaccgccacg ttgaacaggc gcttaacgag acgccagaga agctgaatcg cgatcgtcgc
     7381 ctggtgctgc taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc
     7441 cctgaaagat attcttcatg ggtgagttat tacgaaggga taaagctcaa tccactggca
     7501 ttgcgtaaac cggatgcggc ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat
     7561 caggcctaca tcgttcctgc aatttattga ttttgcaaga ttttgtaggt cggataaggc
     7621 gttcacgcca catccggcaa aaacaacgag ccaataaaaa taccggcgtt atgc
//
LOCUS       DAJTUN010000153         7460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTUN010000153 DAJTUN010000000
VERSION     DAJTUN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.153"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..188)
                     /gene="fecD"
                     /locus_tag="OO470_003498"
     CDS             complement(<1..188)
                     /gene="fecD"
                     /locus_tag="OO470_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Ferric citrate binds FecA and is transported across
                     the outer membrane while transmits a signal across the
                     cytoplasmic membrane protein FecR. FecR transmits a signal
                     across the membrane and activates the cytoplasmic FecI
                     that directs the RNA polymerase to express the fecABCDE
                     operon (which encodes the ferric citrate outer membrane
                     receptor and the ferric citrate ABC transporter), as well
                     as fecIR. FecD is one of two (along with FecC) integral
                     membrane protein components of the iron dicitrate ABC
                     transporter; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6252621.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGA"
     gene            complement(185..1183)
                     /gene="fecC"
                     /locus_tag="OO470_003499"
     CDS             complement(185..1183)
                     /gene="fecC"
                     /locus_tag="OO470_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6252622.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(1180..2082)
                     /gene="fecB"
                     /locus_tag="OO470_003500"
     CDS             complement(1180..2082)
                     /gene="fecB"
                     /locus_tag="OO470_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6252623.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(2127..4451)
                     /gene="fecA"
                     /locus_tag="OO470_003501"
     CDS             complement(2127..4451)
                     /gene="fecA"
                     /locus_tag="OO470_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6252624.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(4538..5491)
                     /gene="fecR"
                     /locus_tag="OO470_003502"
     CDS             complement(4538..5491)
                     /gene="fecR"
                     /locus_tag="OO470_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6252625.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(5488..6009)
                     /gene="fecI"
                     /locus_tag="OO470_003503"
     CDS             complement(5488..6009)
                     /gene="fecI"
                     /locus_tag="OO470_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6252626.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            6307..6489
                     /locus_tag="OO470_003504"
                     /pseudo
     CDS             6307..6489
                     /locus_tag="OO470_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6483..6587
                     /locus_tag="OO470_003505"
                     /pseudo
     CDS             6483..6587
                     /locus_tag="OO470_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6927..7184
                     /locus_tag="OO470_003506"
     CDS             6927..7184
                     /locus_tag="OO470_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR6252627.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         1698 a         1975 c         2061 g         1726 t
ORIGIN      
        1 gcaccgacaa acagtgccag cagcaagcgc ggcagtcggt actccatcaa tacataataa
       61 tgctcgcgtc cggcctgcca gtcggtcagc agcgcgcgcc acggcacggg gatcactccc
      121 atatggagtg ataacagcgc acagcccgcc agggcaaggg tgatgaaaat aaccagcgca
      181 attttcatcc tcgcctcctc acaagccaga caaagcaagg gctgccaatc agcgccagca
      241 ctgcgcctgc gggcagatct ccggggaagg ccagcgcgcg tgcgagtaca tctgccagca
      301 gcatcagcgt ggcccccagc agcatgctca ctggcagtac gttgcgctga tcgaagcctg
      361 cccagaagcg cgccagatgt ggcaccagca gaccgataaa cgccaccgga cctgccacgc
      421 tgacgcacgc accaaccaga agcagcacta acatattgat gaccaaacgt agcctcgtca
      481 ggttcactcc cagcgtatgg gcggtgctgt cgctgaggtt gagcaggttc agttgattcg
      541 ccagcagtaa cacgacaggg actgcagtga ccaccaccgg caagagctgc cagacatcct
      601 gccagcgggc gtgggacact ccgcctgcca gccagtaaaa gatgccgtaa gcatgatctt
      661 cggccagcag cagggtgatg cgggtaaggc ccatacaaaa ggccgacagc gcgatacccg
      721 cgaggatcag tttgtttctg tcatgggtat gacgaaatcc gcctcctgcg gtcatgacca
      781 gcagccagct cacgccgccc ccgcatgccg cgatgaacga cagagaatag cctgcaatcg
      841 gcgtcggact cagcgcgctg gtaagcgcca tagccagcgc cgcgccgctg ttaatgccga
      901 gcagtgaagg agaggccatt gggttgtggg tcagggtttg cagcagcgtg cccgcgagcg
      961 ccaggcttgc gccgatcaga acggcgacca ggcttcgtgg caaacgaagg ttttgcacca
     1021 gcgcttctgg tagcgttggc gtgtgtccag gcagcagggc gcgggttgca tctgctccgg
     1081 aaacaggaat ggccgagtag caaaacagac tcagccagaa aataataata agtgctgcaa
     1141 cgggaagccc ccacagcagc atcgggtgtt ttatcgcggt catttcacaa cggtaagcgg
     1201 ctgatggtgg aagattttta ccgtgtcagc ggcaatacgc tctgcagcaa aaataccgcg
     1261 catccgcgcc caggtgttac tgtcgaccga agcaacctgc tgcttctgcg cggcggttaa
     1321 catctgccag agcggatctt gttgccagcg tttaacaatg ctctcttcgc gatagtgggc
     1381 aaccagcagc caggcaggat tgaccgccag cagttgctcc aggccgatgg acggcatgga
     1441 cgaacccgcc atcgcagcgg gaacgttcag ccccagagag gccagcacgc tgccggtcca
     1501 ggtctcctga gtatgcaggt tgaattgctg ttcgcgtgat gtgccaaagg ccacgcgtgt
     1561 ccctttggga agctggctgg cccactgcgc catcctctct ttatgttgtt ccagacgtgc
     1621 ctgcatctct cgctttttac ccaccatttc gccgatgata gccgcagatt gcaaattttc
     1681 agcgtaggtt tcgttgcggg acttaagcag cagtaccggc gcgatttgct gcaaggcgat
     1741 gtaaaccccc gcatggcgac tgctgtcggc aatgatcagg tctggtttca gagcggcaat
     1801 ggcttccagg ctcggctgcg cgcgcgttcc gacggactgc cacggtttca ggtgcgcacg
     1861 cacttcgggc aggatgcgtt ttgcatcgtt atcgtcggca ataccgatcg ggctgacgtc
     1921 cacggcggcc agcgcatcgg cgaacgagag ttccagcacc acaatccgtt gtggcgtttt
     1981 ttcgagtgta aacgtgccgt gttcgtcctg aaccatggcg gcaaaggcgt ggctgatcac
     2041 cagcagcagg cctgcaaaaa gaaaacggat aaatgccaac ataatcacat tccagctaaa
     2101 agcccggcaa gccgggcgtt aacacatcag aacttcaacg acccctgcat atacagcgtg
     2161 cgcggctggc ctgcatagat gcctttgttg ttgtcgtcat aagagcggat gaagtagtcc
     2221 tggtcgaaga tgtttttcac accgaatgcc aggttcagat ctgccatctg cgggccaaag
     2281 tcatacgcca cgcgtgcacc ccagagcatg aagccgggaa tgcggccggt actgccgtcg
     2341 gcgctctctt tcaccgtatt ggcgttatcc gcaaactggc tggactggaa atcgctgttc
     2401 agattgaacg tccagtttcc tggcttgtag tccacgccca gcgtgccttt atgtttcggg
     2461 gagaatggta ccagattgcc gtaggtgtcg cctttctcgc ggatttccgc gttcacatac
     2521 gcatagctgg cgtagatgga aacgttatca agcgttggcg ttagcgtacc cagatcgtaa
     2581 cgtgcctgcg tttccagccc ggtatggcgc gttttgccac gtgcagtgac ggtgtcgttg
     2641 gtctggttgg agtcgtactg attgttaaag ttaatcagga acagccccat ttccgccgtc
     2701 agcgcgccgt cgtcgtagcg ggtaccgagt tcccaggttc gcgctttttc cggttccaca
     2761 ttgccgcttt gcacagcctt gccaatctgg ctgtactgta cggtgccgaa cgagccttca
     2821 gtgtttgcat aaagattcca gctgtcagtc aggtgataga gcacgttcaa cgccggaagc
     2881 ggtgcgttat agctcacttc ttcgtgcgtg cctgtgatgg cgttgttctg gtatgactcg
     2941 atatgttcga aacgcatacc cggcgtgatg gtccagttgc cgatgtcgat tttgtcatcc
     3001 agataccagg cgtgcgcctc ggtgccggaa cgcgtatcgc ggtcgtaagg gcttgagccg
     3061 gacggcaact gcccgctgct ggtggcggtg tagtaacgca tttcatgcgt tgattcattc
     3121 acatagcgat agcccacgcc cacttcgtgc gcggaagggc cgatcataaa gatctggctg
     3181 tagcgtggct caataccgcg cacccagtag ttacgcggcg agagggtgat gcgtttgcct
     3241 tgctccaggt agccgctgcg cagggtttgg gtgtagaacc cctgaatgtt gaatttatgc
     3301 tggctgtctg gctggaactg gtagcccagg ctcgccagct tgcgacgacc ccagaagcgg
     3361 tcatacgggc gggtggattg ccagcgatcg gcgtcgtaat ccgcgcgaga caggccaccg
     3421 ggcatgtcgg cttcaccgtc gtaatattgc agcaggctgt tgaaggtgtg cacctcatcc
     3481 ggcgcatatt tgcttttcag catcaggtcg tcgatgcggg tggcgctgtg ctcgcgccag
     3541 tcactgccgc gcgtgccgga gtagagcagc gcggtgccaa aaccgttgtc cgctgtgccg
     3601 cccaccatca ggttgtgcgt ctctttcggg ttgttttgtg aagaggttgg gctgagctga
     3661 ccttccacgc ccgcctcgat accaaagtcc tgcggaatgg cacgggtaac aaagttcacc
     3721 acgccgccca cgctctgcgg tccgtaacgc accgcaccac caccgcgtac cacgtcaatg
     3781 gcatccatgt tgccgagcga aatgggagcc agtgaaagct gcggctgacc gtaaggggcg
     3841 aaggggacgg ggatgccgtc catcaggacg gtcgagcggc tggcgaggcg cgggttcagg
     3901 ccccggatgc caaagttcat cgccaggtcg tggctgccgg tgccgttgtt ttccggcgcg
     3961 ctgacgccag ggatgcggtt aagtacctca cgcatggtgg ttgcgccggt tttggcgaaa
     4021 tcctcacggc ggatcacgtc acgcgcgcca gcatgttcaa atacgtcgtt ttcacgcgca
     4081 tcacccagcc agtcgccgac cacggtcagg gcatcttctt ttggtgcggg cgcgggctcc
     4141 agcgtccagc tgttatttcc cagcggtttt acctgcagtc cgctgccgtc cagcagttgt
     4201 tgcaggccgc tctcgacgtc gtaatcgccg tgcaggccgt tgctctgctt gccgcgcgtc
     4261 aggctggcgt caaccgagag ggtaaatccg ctgtgtgcgg catactgatt gagcgcttta
     4321 tcgagcgatc ccggtgcgat attaacctgt gcagcaaaag cggaaaacga gagaccggcc
     4381 agcggcagca ggctcaggcg aatggcgtta accaaaggtg ttgttttacg aaaaacgcgt
     4441 aacggcgtca taccttcccc atcatcattt ttgttgtgtt cagctatgag tcgaacgaga
     4501 agggtaaaaa ggacaatcga aataagaatt attttcctta cagtggtgaa atgtttatcc
     4561 agtaccgcgt aatagactga attttaacgg gaagcgtttg cgcgataacg ttcaggatcg
     4621 catcggtatt tttcagcggg aacgtcccgc tcaggcgcag cccggcaacg gcgggatcgc
     4681 agcgcagcac gccgttgcgg taacgggtta gcgtggctat cacctcaccc agcggtttat
     4741 cgctgaagct caggatgtcc ttcgtccagc ttgtactctc gtcatccagc ggtttcactg
     4801 cgccaaactc agaggcgctg aactgcaggc tttcaccagc gttcacgatg cgtttttgcg
     4861 cgggggcact ggcgagaagc acttccacag cgtgctgctg aacgtcaagc tgcgtgaaat
     4921 tatcctgctg gcggacggta aattctgtcc ctaaagcagt gagctggccc tgacgggtca
     4981 ggacacggaa ggggcgttgc agggcatctt tcgcggtagt aatggcgatt tcaccgtacc
     5041 agagccggac ggtgcgctga tgcgcatcaa aacgcacatc cgccgcgctt tgggtattca
     5101 gcgtgagcag ggagccatct tccagttgct gacggctgac tgtgcctttg gcggtgcggt
     5161 aatctgcccg cagaccttcg ccggtttccg actgccagag ctgccagcct ccgccagcgc
     5221 cgagcaacag cagtaatcct ttcatcacgt gacggcgggt gaggcgggta tcgtgcaacg
     5281 cccggctggc aacgtcgcca ggcacaccgc caagctggtt gcgcaggttt tcaacctgct
     5341 gccaggccca ctggttatcc tgatcctgtt cataccactg ttgccagcgc gcttcctgtt
     5401 gtgggctgac gcgctcgccg cttagcacgg cataccagtg ggaagctgaa cgcagcgcct
     5461 gacggcggga atcggttaac aaaggattca taacccatac tccagacgga acagcaggca
     5521 gtgctcgacg gctttcgcca cgtatttttt cacggagctg atggaaacac cgagtttgtg
     5581 cgcaatctcg ctgtatgtca gaccatccag ttgcgaaagc agaaacgctt cacgtgtttt
     5641 gccgtttagc ccgtccagca tgctgtcgag gagttgtagg gtctcgagtt ggctttcgcg
     5701 ttcctcaggt gaaggcgctc ccccctccgg cataagcgcc agcatctcca gatacgcttt
     5761 ttccagcgcg tttcggcgaa acaggtccac catcacgcgt ttggcgatag tgcagaggaa
     5821 ggagcgagga tcgcggatcg tcgagagcgt ttcgctgacc attacccgca aaaaagtgtc
     5881 ctgggcaatg tcatctgcat caaaagcaga ctggagtttg cgcgtcagcc agcttttcaa
     5941 ccagccgtga tgtgtgccat aaagcgactc gaacgttaag gaagctgtgg tagtggcgcg
     6001 gtcagacatg cggagtgcat caaaagttaa ttatcacgta gtcatattaa tatgagaatg
     6061 gttatcatta caattggaaa taaaattgtt tccaatagac atttttaaca tgttgttttt
     6121 ctaagtgtta taaggtaggt ataaaatggg atggagcctc tgcttctgac atgtgtcggt
     6181 cagaatgact catgatgtgg tctgctatta ttgacatcct cactgcccta aaggatgggg
     6241 atttcggtaa tgatcttacc cagcaatagt ggacacgcgg ctaagtgagt aaactctcag
     6301 tcagaggtga ctcacaagac aaaacagttt caaccagtaa aaaaacgtaa acaccattcg
     6361 catgaatttc gtagttaaga tctgaagctt gcagaatgca tcggtgttac tgccggagtc
     6421 cgtgaactta gtctgcatga atcaccacag ctttacatct gtcgcagtaa acagcaaaat
     6481 cagcaaactt acttaattat atcgagtgcg attacaatcg ctggcgatgg tacagttggt
     6541 gtggcggtct cagtccggaa caatttgaaa atcataatct cacttaaaac ggcatccatt
     6601 ttacaaaaga tggccgtctg tgtatttgta atgaatacct tctacatgtt tagagtgcat
     6661 aatccacgac aaggcgatat cctaaaaatt agctagcagg caactttgat ggcatagata
     6721 atgtttttag taaaaaagag ttgactgaat ataatacagc ctcttcctac ataagaagga
     6781 atgtgctcac aaaaaaaaac cattagtctg cttcctgttc ttacaataat cagtattatg
     6841 tattttattt atatagcaaa atatgcttgt tacacctgtc ataattaggc atggtaaaat
     6901 tattgactga accctggggg agaaagatgc acagtgatta ttataatatg gtttttggtg
     6961 aaaaattggc caatatttta tatgaagcta actctcaatt tttttacgag agaaatgtca
     7021 tagaagaggc agtcaacgct ttattctgtg agcgtgaaat aataaacaac aagaacataa
     7081 taaagaagct gatgttcttt ttgtcagatg ttaatcacac caagaaagat gttgttcaga
     7141 gcgcacttaa tattatcatt gacattacat caggtgatat ttgagatttt acataaaaga
     7201 taatctctca aggagtctta tctgaaaacg aaatatatag tccctctcgt tttgtaatat
     7261 cgattggctt tgttgaatcg gttacgtacc ccggagccgg tcatattttc aggtaataca
     7321 cacgctttca gacatttcgg ctttcgtcat ttttgtatca tgggcatagc ttctgctacc
     7381 tgaccatcat aatcacgcag cttcagtgaa gcagtgacca cctgtttaac ccggtgtatc
     7441 gttatgatct tatccagcaa
//
LOCUS       DAJTUN010000154         7381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTUN010000154 DAJTUN010000000
VERSION     DAJTUN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.154"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..205
                     /locus_tag="OO470_003507"
     CDS             <1..205
                     /locus_tag="OO470_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1158 family protein"
                     /protein_id="HCR6252628.1"
                     /translation="LMAFLSCLLLPAPALGLTLAQKLVTMFHLMDLSQLYTLLFCLWF
                     LVLGAIEYFVLRFIWRRWFSLAD"
     gene            270..638
                     /locus_tag="OO470_003508"
     CDS             270..638
                     /locus_tag="OO470_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6252629.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            732..1385
                     /gene="nfsB"
                     /locus_tag="OO470_003509"
     CDS             732..1385
                     /gene="nfsB"
                     /locus_tag="OO470_003509"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6252630.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1493..2740
                     /gene="ybdG"
                     /locus_tag="OO470_003510"
     CDS             1493..2740
                     /gene="ybdG"
                     /locus_tag="OO470_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6252631.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2808..4184)
                     /gene="pheP"
                     /locus_tag="OO470_003511"
     CDS             complement(2808..4184)
                     /gene="pheP"
                     /locus_tag="OO470_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6252632.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4285..>7381)
                     /gene="cusA"
                     /locus_tag="OO470_003512"
     CDS             complement(4285..>7381)
                     /gene="cusA"
                     /locus_tag="OO470_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6252633.1"
                     /translation="LMGALFLSIWGTWTIINTPVDALPDLSDVQVIIKTSYPGQAPQI
                     VENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSRVLEYLNQVQ
                     GKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYELKTIPDVAE
                     VASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELAEAEYMVRAS
                     GYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIAELNGEGEVAGGVVILR
                     SGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAIDNLSGKLLEEFIVVA
                     VVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMSLGGIAIAVGAMVDAAI
                     VMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPALFISLLIITLSFIPIFT
                     LEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIPPESSNPLNRFLIRV
                     YHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEGDLLYMPSTLPGISA
                     AEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVETTIQLKPQDQWRPGM
                     TMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTVLADIDAMAE
                     QIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVADVQLFVTSAVGGAMV
                     GETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLADVADVKVSTGPSMLK
                     TENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAFSGQFELLERANHKL
                     KLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLWWMGFHLSVATGTGF
                     IALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALYHGAVLRVRPKAMTV
                     AVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIPAAYKLMWLHRHRVR
                     N"
BASE COUNT         1802 a         2065 c         1794 g         1720 t
ORIGIN      
        1 gctgatggct ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc
       61 acaaaaactg gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt
      121 ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg
      181 acgctggttc tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg
      241 cttattctcc ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga
      301 aacgcctagc tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg
      361 atgtttttaa aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct
      421 ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca
      481 ttaagccagg gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa
      541 tctccgaagc gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc
      601 tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt
      661 ttctcgctta ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac
      721 atggagtctt tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg
      781 atgccagcaa aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca
      841 gcccatccag caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta
      901 aagcgcgtgt tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg
      961 atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc
     1021 tggttgttga ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga
     1081 acgataaagg tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag
     1141 agtggatggc aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc
     1201 tgggtctgga cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg
     1261 gtctgaaaga gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg
     1321 aagattttaa cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag
     1381 tgtaattctc tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc
     1441 ataaccctgt ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga
     1501 tttaatatcc caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt
     1561 gatgattttc ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt
     1621 ggtactgcgg accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat
     1681 tacccagaat aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat
     1741 tcaggcggta ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc
     1801 gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt
     1861 gaatctggcg cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg
     1921 gattaaactg atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca
     1981 gtcgccagcg attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt
     2041 taaagatccg attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa
     2101 actgggcgac tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg
     2161 gttaaccacc gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc
     2221 tctggtttct gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat
     2281 taagcgcagt atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca
     2341 acgtctgaat aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa
     2401 tgagtggaat cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa
     2461 tattggaacc tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa
     2521 agatatgacc ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat
     2581 ctacgcgttt actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt
     2641 cgatcacata tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg
     2701 caatgatatt cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc
     2761 gaatggatga gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc
     2821 agcgttttaa atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag
     2881 cgcatatcat ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga
     2941 tagttgccga acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc
     3001 gccgcgcgaa aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct
     3061 accaccagcg ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc
     3121 accagcgaag tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg
     3181 acgcgagtca aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg
     3241 ttagagtaaa ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc
     3301 gcagaagcta ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg
     3361 gatttcactt ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac
     3421 agcaggatgc gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc
     3481 gcttcagcgg ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc
     3541 agcgacaaaa tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg
     3601 tcgatactgg ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc
     3661 ataccgatga tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag
     3721 cgcacgttca ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac
     3781 gttggaacat ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt
     3841 cccaccagca cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc
     3901 cagtatttat aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg
     3961 ccaagctggc gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca
     4021 gccggacccg ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg
     4081 ccacccagcg caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct
     4141 tgattcgacg cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg
     4201 tcttttttgt tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca
     4261 cacggttaaa cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag
     4321 cttatacgcc gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat
     4381 catcggtgcg gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg
     4441 cagcagaccg gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc
     4501 gccgtgatac aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga
     4561 cggctcggcc tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc
     4621 gaccccggca agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca
     4681 ccacagtaac cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc
     4741 ttcgcccacc cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg
     4801 caccatgagt ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc
     4861 cacgctggtg cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac
     4921 caccgacacc atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt
     4981 ctcggttttc agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt
     5041 gatttgctgc ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg
     5101 ccagctttgc ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc
     5161 aaccatcgcc ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc
     5221 gtaacgtgcg gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg
     5281 ctcagcaagc gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc
     5341 catcgcgtcg atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat
     5401 gccggttgag agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc
     5461 cggcagacgc acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg
     5521 ccactgatcc tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc
     5581 ggtggcggtt tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat
     5641 aattagcttg tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag
     5701 cgtcgatggc atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac
     5761 tttattgagc ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt
     5821 cggccagtgc agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag
     5881 cggattactg ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat
     5941 cactacgatc gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg
     6001 gccaaacaga cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat
     6061 aatcagcaga ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg
     6121 ccagcgcgtt ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg
     6181 tttatgcgca ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc
     6241 aatgccaccc agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc
     6301 aatacacaac cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag
     6361 aaacagcgcg cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt
     6421 gtcgatggcc cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc
     6481 cggcagacta cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc
     6541 gtttttgccg gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc
     6601 ggcaatgccc cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac
     6661 gggcacgcca ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag
     6721 atagccgctg gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc
     6781 ttcctggttt gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc
     6841 caggcgctga ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac
     6901 ttccgcaacg tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg
     6961 taaatcggca agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc
     7021 aacacccgtg gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac
     7081 ctggttgagg tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa
     7141 aatgacatac acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg
     7201 caccgacaac atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc
     7261 ctggccggga tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc
     7321 cactggcgta ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag
     7381 c
//
LOCUS       DAJTUN010000155         7300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTUN010000155 DAJTUN010000000
VERSION     DAJTUN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.155"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..904
                     /locus_tag="OO470_003513"
                     /pseudo
     CDS             1..904
                     /locus_tag="OO470_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            909..1874
                     /gene="rbsC"
                     /locus_tag="OO470_003514"
     CDS             909..1874
                     /gene="rbsC"
                     /locus_tag="OO470_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6252634.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1899..2789
                     /gene="rbsB"
                     /locus_tag="OO470_003515"
                     /pseudo
     CDS             1899..2789
                     /gene="rbsB"
                     /locus_tag="OO470_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2915..3844
                     /gene="rbsK"
                     /locus_tag="OO470_003516"
     CDS             2915..3844
                     /gene="rbsK"
                     /locus_tag="OO470_003516"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6252635.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3848..4840
                     /gene="rbsR"
                     /locus_tag="OO470_003517"
     CDS             3848..4840
                     /gene="rbsR"
                     /locus_tag="OO470_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6252636.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4806..6233)
                     /gene="mdtD"
                     /locus_tag="OO470_003518"
     CDS             complement(4806..6233)
                     /gene="mdtD"
                     /locus_tag="OO470_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6252637.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6256..6948)
                     /locus_tag="OO470_003519"
     CDS             complement(6256..6948)
                     /locus_tag="OO470_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6252638.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1852 a         1764 c         1928 g         1756 t
ORIGIN      
        1 tgaaagaaat cttcgagatt tgcgatgacg ttaccgtttt tcgtgatggg caatttattg
       61 ctgagcgcga agtggcatca ctgaccgaag attcgctgat tgagatgatg gtgggccgca
      121 agctggaaga tcaatatcca cacctggaca aagcgccagg agatatccgc ctgaaagtcg
      181 ataatctctg cggacctggc gttaacgatg tctcttttac tttacgcaaa ggcgaaattc
      241 ttggcgtctc tggtttgatg ggcgcaggtc gtaccgaact gatgaaagtg ctctacggcg
      301 cactgccgcg caccagcggt tacgtcaccc tggatgggca tgaagtcgtt acccgttcac
      361 cgcaggatgg cctggcaaac ggcattgtgt atatctccga agaccgtaaa cgtgacggtt
      421 tagtgctggg catgtcagta aaagagaaca tgtcgctgac agcgctgcgc tacttcagcc
      481 gcgctggcgg cagtttgaag catgccgatg aacagcaggc tgtgagtgat ttcattcgtc
      541 tgtttaatgt gaaaacgccg tcgatggaac aggcaattgg tctgctttcc ggtggcaatc
      601 agcaaaaagt ggcgattgcc cgcggtctga tgacaccccc aaagtattga tccttgatga
      661 gcctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga ttaaccagtt
      721 caaagccgat ggcttgagta tcattctggt gtcatcggag atgccagaag tattaggcat
      781 gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta ctcgtgagca
      841 ggccacccag gaagtgttaa tggctgtcgc tgtgggcaag cttaatcgcg tgaatcagga
      901 gtaaaaaaat gataacccag actgtctctg gtcgccgtta tttcacgaaa gcgtggctga
      961 tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg acgttaagcc
     1021 cgaacttttt caccatcaat aacttattca atattctcca gcaaacttcg gtgaacgcca
     1081 ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg catagacttg tcggtaggtt
     1141 ctctgttggc gctaaccggc gcagttgctg catctatcgt cggcattgaa gtcaatgcgc
     1201 tggtggctgt cgctgctgct ctcacgttag gtgccgcaat tggtgcggta accggggtga
     1261 ttgtagcgaa aggtcgcgtc caggcgttta tcgctacgct ggttatgatg cttttactgc
     1321 gcggcgtgac tatggtttat accaacggta gcccagtgaa taccggcttt actgagaacg
     1381 ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg ccagtctgga
     1441 tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt ctggggcgtt
     1501 acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc aacgtcaata
     1561 aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc gggatcattg
     1621 aagtggcgcg tctctcctct gcacaaccca cggcggggac tggctatgag ctggatgcta
     1681 ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc attgttggga
     1741 cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg ttaggtgttt
     1801 cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg ctggtagaca
     1861 acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa aaactggcta
     1921 ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg gcaaaagaca
     1981 ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg aaagatggcg
     2041 cgcagaaaga ggcggataaa cttggctata acctggtggt gctggactcc cagaacaacc
     2101 cggcgaaaga gctggcgaac gtgcaggact taaccgttcg cggcaccaaa attctgctga
     2161 ttaacccgac cgactccgac gcagtgggta atgctgtgaa gatggctaac taggcgaaca
     2221 ttccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc cacattgcat
     2281 ctgataacgt actgggcggc aaaatcgccg gtgattacat cgcgaagaaa gcgggtgaag
     2341 gtgccaaagt tatcgagctg caaggcattg ccggtacatc cgcagcccgt gaacgtggcg
     2401 aaggcttcca gctggccgtt gctgctcaca agtttaatgt tcttgccagc cagccagcag
     2461 attttgatcg caccaaaggt ttgaacgtaa tgcagaacct gttgaccgct catccggatg
     2521 ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc gcactgcaaa
     2581 ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat ggcgaaaaag
     2641 cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga
     2701 aaggcgttga aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatctgg
     2761 ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac
     2821 ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc
     2881 taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt
     2941 tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga
     3001 aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt
     3061 ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat
     3121 tggcgagagc gttcgccagc agctcgccac tgataacatc gatattactc cggtcagcgt
     3181 gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt
     3241 catcggtatt catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg
     3301 tgagcgtatt gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt
     3361 gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc
     3421 ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac
     3481 ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc
     3541 gcaggtactg catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt
     3601 atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga
     3661 taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa
     3721 accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa
     3781 aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag
     3841 gtgacgcttg gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt
     3901 ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc
     3961 ggcgattaaa gaactcaatt acgcgccatc agctctggcg cgtagcctca aactcaatca
     4021 aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt
     4081 gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga
     4141 aggcgatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg
     4201 cttactgtta ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc
     4261 gacagtgcct accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca
     4321 ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac
     4381 ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg
     4441 ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg
     4501 tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg
     4561 tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata
     4621 tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact
     4681 ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga
     4741 gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt
     4801 acaacttact ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc
     4861 aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata
     4921 atgcccatcg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct
     4981 tcataaatgc gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc
     5041 gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg
     5101 atggtattca tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag
     5161 atcagcatcc atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca
     5221 ataatcaccg tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta
     5281 accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata
     5341 aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg
     5401 gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat
     5461 aaatctaatg aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt
     5521 aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg
     5581 aatagctcta ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg
     5641 atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg
     5701 cccgcaatac ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc
     5761 agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa
     5821 ttcaataccg gaagaagttc attacgagga taagcacgca gtaaggccgg ccgagcaaca
     5881 ggcatcatca ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc
     5941 gaattagaaa gtgcgtaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa
     6001 atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc
     6061 gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga
     6121 gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaagaaggcc
     6181 atcgccgcga tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt
     6241 cctgttatcg ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc
     6301 cgcgtcgcca tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat
     6361 cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc
     6421 gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggccatatc
     6481 gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa
     6541 cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg
     6601 cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc
     6661 tgttatccac caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac
     6721 ccgagtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc
     6781 ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa
     6841 aatggtgccg ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac
     6901 atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc
     6961 tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg
     7021 gcaaattgct tgttttatgg cacattaacg gggtattttg ctgaaaaaat gcgcggtcag
     7081 aaaattattt taaatttcct cttgtcaggc cggaataact ccctataata cgccaccact
     7141 gacacggaac aacggcaaat acgccgccgg gtcagcgggg ttctcctgag aattccgaca
     7201 gagaaagcaa aaataaatga ttgactctgt agcgggaaag cgtattacgc acacccccgc
     7261 gccgctgaga aaaagcgaag cgcactgctc tttaacaatt
//
LOCUS       DAJTUN010000156         7242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTUN010000156 DAJTUN010000000
VERSION     DAJTUN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.156"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(2..94)
                     /locus_tag="OO470_003520"
     tRNA            complement(2..94)
                     /locus_tag="OO470_003520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO470_003521"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO470_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6252639.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO470_003522"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO470_003522"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6252640.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO470_003523"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO470_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6252641.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASCSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO470_003524"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO470_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6252642.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO470_003525"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO470_003525"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6252643.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO470_003526"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO470_003526"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6252644.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1681 a         2034 c         1751 g         1776 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacagctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aa
//
LOCUS       DAJTUN010000157         7209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTUN010000157 DAJTUN010000000
VERSION     DAJTUN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.157"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1005
                     /gene="ydfJ"
                     /locus_tag="OO470_003527"
     CDS             <1..1005
                     /gene="ydfJ"
                     /locus_tag="OO470_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6252645.1"
                     /translation="VTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNC
                     GTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVND
                     SNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTD
                     ALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIM
                     VALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFC
                     TMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL
                     "
     gene            1094..2554
                     /locus_tag="OO470_003528"
     CDS             1094..2554
                     /locus_tag="OO470_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6252646.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQRDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2590..2793)
                     /gene="ydfZ"
                     /locus_tag="OO470_003529"
     CDS             complement(2590..2793)
                     /gene="ydfZ"
                     /locus_tag="OO470_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6252647.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2971..3657)
                     /gene="ydfH"
                     /locus_tag="OO470_003530"
     CDS             complement(2971..3657)
                     /gene="ydfH"
                     /locus_tag="OO470_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6252648.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3746..4492)
                     /gene="ydfG"
                     /locus_tag="OO470_003531"
     CDS             complement(3746..4492)
                     /gene="ydfG"
                     /locus_tag="OO470_003531"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6252649.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4629..6674
                     /gene="dcp"
                     /locus_tag="OO470_003532"
     CDS             4629..6674
                     /gene="dcp"
                     /locus_tag="OO470_003532"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6252650.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6719..7189)
                     /locus_tag="OO470_003533"
     CDS             complement(6719..7189)
                     /locus_tag="OO470_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6252651.1"
                     /translation="MENLTSALSQRLIADQLRLTTAESCTGGKLASALCAAEDTPKFY
                     GAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGG
                     PEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAVRFALAQLLQLLL"
BASE COUNT         1778 a         1714 c         1859 g         1858 t
ORIGIN      
        1 gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg tgccggtacg
       61 atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt tgtggctatg
      121 ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt cttcattctc
      181 agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctagcgag cgttgtcgtg
      241 atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt tgagaaggtt
      301 aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt ccagagcaaa
      361 tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg tttaattcag
      421 actttccttg caggctattt agtacagacg ttattgttta acaaagcaat tccaacagat
      481 gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg ttggttatcc
      541 gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat tgtgctggcg
      601 tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat tatggttgca
      661 ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa cattaccatg
      721 gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa agaaattggt
      781 ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac catgacggaa
      841 tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt aatctctgcg
      901 ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc tgcggaagat
      961 caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct caatcccttc
     1021 tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc atatttaaat
     1081 ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc ctgtttatga
     1141 tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc accgtgcgca
     1201 tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg gatattatga
     1261 ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcgag ataatcttta
     1321 taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg tcgttaaaaa
     1381 agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt gtgaaccgca
     1441 aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc cggcgaccgg
     1501 acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc accagccgaa
     1561 aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag gacttcccgc
     1621 atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc gtgacgttgt
     1681 cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag ataacgtgac
     1741 tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata cgctggcgaa
     1801 aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac ctttccgcca
     1861 gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag cgggagccga
     1921 actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca acggcagtca
     1981 ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg actgtatgga
     2041 agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag cgccgacgct
     2101 gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac gctatagcaa
     2161 cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga aattgccaca
     2221 gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg atctgctggc
     2281 gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg gaaatccgat
     2341 agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta gtgccgaagg
     2401 gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg atttgccaga
     2461 caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag cgcatggcgc
     2521 gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc atcggcggca
     2581 ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt tctcttcaca
     2641 accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac cttcagtatc
     2701 aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc tgccagtggt
     2761 gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc tgatttacag
     2821 tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata ttttttgttt
     2881 ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg ggaaaagcct
     2941 ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt cgctgttttc
     3001 ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca ttgcacgttc
     3061 taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct ggcgtaacag
     3121 catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa cggtcgcttt
     3181 caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca ggagttgatg
     3241 gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg caatgcgttg
     3301 ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc tcgccgcccg
     3361 acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca cctgggccat
     3421 ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc cgttttccgc
     3481 cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa cagaaacttc
     3541 tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc gacgaagaat
     3601 acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga cggtcattcg
     3661 ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac aagcaataac
     3721 ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg gcatagcttt
     3781 gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc gttgacaccc
     3841 accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga taggtttttt
     3901 ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca cccaccagac
     3961 ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc agattcaggc
     4021 taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca tacggccagc
     4081 tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc atacccggta
     4141 agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc gtttcccagt
     4201 cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca ttatttacca
     4261 ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca atagcggcgc
     4321 ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct gttaactcct
     4381 gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga ataaaacgac
     4441 gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc atctcactgt
     4501 tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg ggtaatactg
     4561 gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct cgcgttcagg
     4621 agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg tatctggctc
     4681 ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag ggaatgcaac
     4741 aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat ttcaacaata
     4801 ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc gtcttttttg
     4861 cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag ttttccgctg
     4921 aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg cgggtagatg
     4981 ctgtctggca gctccgtgaa tccctggggc ttgatagtga atccatccgc ctggtggagg
     5041 ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat aaagcaaaat
     5101 taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag cggttacttg
     5161 cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca ggaatgagtg
     5221 agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat aacaaatggc
     5281 tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc gatcgtgcga
     5341 cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat gccaatgata
     5401 cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa ctgcttggtt
     5461 ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca gaagcagcac
     5521 tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat gaattagcct
     5581 ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg tgggactggg
     5641 cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg cagctcaagc
     5701 catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg aatcagctct
     5761 tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac gttcgtgtgt
     5821 gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat ttcttcgccc
     5881 gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca acgcttaatg
     5941 aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc ggtgagcctg
     6001 cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat acgctgcacg
     6061 gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg cgtgattttg
     6121 tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta ttcgctcgct
     6181 acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag aaaatgcgta
     6241 atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc gcacttctcg
     6301 atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat gattttgaat
     6361 tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc tatcgcagca
     6421 gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct tatctgtgga
     6481 cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga ttaacgcgtg
     6541 aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa gatctggaac
     6601 gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg caacatcgtg
     6661 gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc cagcctggtt
     6721 atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct aataccgttt
     6781 cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata tgccacgcga
     6841 accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg tagccggtaa
     6901 tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt tctgctgcca
     6961 ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg aggattttca
     7021 tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt gtatcttcag
     7081 ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta gttaagcgta
     7141 attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata gtgtcggtat
     7201 ctgcgagtt
//
LOCUS       DAJTUN010000158         6996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTUN010000158 DAJTUN010000000
VERSION     DAJTUN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.158"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..275
                     /locus_tag="OO470_003534"
                     /pseudo
     CDS             1..275
                     /locus_tag="OO470_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            272..877
                     /gene="ttdB"
                     /locus_tag="OO470_003535"
     CDS             272..877
                     /gene="ttdB"
                     /locus_tag="OO470_003535"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6252652.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            925..2388
                     /gene="ttdT"
                     /locus_tag="OO470_003536"
     CDS             925..2388
                     /gene="ttdT"
                     /locus_tag="OO470_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6252653.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2431..3444)
                     /gene="tsaD"
                     /locus_tag="OO470_003537"
     CDS             complement(2431..3444)
                     /gene="tsaD"
                     /locus_tag="OO470_003537"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6252654.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIACAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            3682..3897
                     /gene="rpsU"
                     /locus_tag="OO470_003538"
     CDS             3682..3897
                     /gene="rpsU"
                     /locus_tag="OO470_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6252655.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4008..5753
                     /gene="dnaG"
                     /locus_tag="OO470_003539"
     CDS             4008..5753
                     /gene="dnaG"
                     /locus_tag="OO470_003539"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6252656.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            5948..>6996
                     /locus_tag="OO470_003540"
     CDS             5948..>6996
                     /locus_tag="OO470_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; this is the primary sigma
                     factor of bacteria; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6252657.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEE"
BASE COUNT         1656 a         1885 c         1884 g         1571 t
ORIGIN      
        1 tccaccggta ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc
       61 gcgtaaagcc attttgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact
      121 ggagctacgc ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgaccgg
      181 caacagttca gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc gatctcacct
      241 ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa
      301 agctgaagat ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt
      361 gacctgccgc gacgtttgtc accgccgttt gatcgaactg aaacgtccga tcccttacga
      421 tctcaacggc aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gtgacaaatg
      481 ggagatggtc tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt
      541 tattgagcag accggcgtaa aactggtggt tggcaaaggt ggtatggggc cgctgaccga
      601 agaaggctgc cagaaattca aggcgttaca tgtgattttc ccagcaggct gcgcggtgct
      661 ggcggcaacc caggtagaag agattgaaga agtgcactgg acagaactcg ggatgccgga
      721 gtcactgtgg gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca
      781 cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt
      841 ggaagagatc tgcgagcacg tccattacat caaataaccc tcccggagag gctcccccct
      901 ctcctttttc gcaggcataa cacgatgaaa ccttccactg aatggtggcg atatcttgcg
      961 ccgctggcgg tcatcgccat tattgctcta cttccggttc ccgcagggct ggagaaccat
     1021 acctggctct actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg
     1081 ccgggtgccg tggtggcgat ggtgggtatc tccatcatcg ccatactctc tccctggctg
     1141 ctgttcagcc ccgaacagct cgcccagcca ggctttaagt tcaccgccaa atccctctcc
     1201 tgggcagttt ctggtttttc taattcggtt atctggctca ttttcgccgc ctttatgttt
     1261 ggcacaggct atgaaaaaac cgggcttgga cgccgcattg cgctgattct ggtgaaaaag
     1321 atggggcatc gcacgctgtt tctcggctat gcggtgatgt tctccgagct gatcctggca
     1381 cctgtaacac cgtccaactc ggcgcgtggt gcgggtatta tctatcccat catccgtaac
     1441 ctgccaccgc tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac
     1501 atcatgtgga tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg
     1561 gcacctaact tgctgttaat tggactgatg aaaagcgcat ctcacgccac gctgagttgg
     1621 ggcgactggt tcctcgggat gttgccgctc agcattttac tggttctgct ggttccctgg
     1681 ctggcttacg tgctgtaccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca
     1741 gagacggaac tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg
     1801 atggtaggcg cgctggtgct gtggattttc ggcggtgatt atatcgatgc cgcgatggtc
     1861 ggttacagcg tggtggcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc
     1921 agtaataaag cggcatggaa cgttttcttc tggctggcct cgcttatcac cctcgcgacc
     1981 ggactcaaca ataccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagt
     2041 ggttattcgc cgacgatggt gatggtgacg ttgattgtgg tgttttatct actgcgctac
     2101 tttttcgcca gcgccacggc gtatacctgc gccctcgcac cgatgatgat cgccgccgcg
     2161 ctggcgatgc cggaaatccc gctgccggtg ttctgcctga tggttggcgc ggcaattggt
     2221 ctggggagca ttcttacacc atacgccacc ggacccagtc cgatttacta cggtagtggt
     2281 tatctgccaa cggcggatta ctggcgactg ggggcgattt ttgggctgat attcctcgtg
     2341 ttgctggtga ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg
     2401 ccggaaattg ctctccggca cccaatagtt ttacgcagcc ggtaactccg ccagcggcca
     2461 gcgcggacgc acgctaacac cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc
     2521 ggcacaggcg atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt agaacacttc
     2581 gccgcggcgt tttttcatca tttcagccag cttcgcccgt aacgtgcggt tagcactcac
     2641 gccgcccgcc atgaccagtc gcttaaagcc cgtctgatcc aacgctcgct tacacttaat
     2701 catcaacgta tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc
     2761 gtcggtgccg ttgtcacgaa tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa
     2821 atccagcccc ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg
     2881 agccgccatt ttcgacagta acggtccgcc aggataatcc agccccagca gcttcgcggt
     2941 tttatcaaac gcttcaccgg cggcatcatc gatagactcg ccgagcagct cgtactgacc
     3001 aatgccagtc acgctgatta actgcgtatg accgccggac accagcagcg cgacaaacgg
     3061 aaattccggc gggttatctt ccagcatcgg cgctaacaga tgcccttcca tatggtgtac
     3121 cgggattgcc ggaacgttcc aggcaaacgc cagagaacgc cccacggttg cgccaaccag
     3181 cagtgcgccg actaatccag ggcctgcggt ataggccaca gcatcaatgt cttttgccgt
     3241 taaaccagac tcctttagcg ccgcctggat caacggtacg gttttacgca catgatcgcg
     3301 ggaggccagt tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa
     3361 ttggttggct aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga
     3421 agtttcaata cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta
     3481 ggaccaatgc gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc
     3541 ctcaccctta taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat
     3601 tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa
     3661 ttaatcaaag gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac
     3721 gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt
     3781 cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg
     3841 aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc
     3901 cccgagagcg ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag
     3961 gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa
     4021 tcccacgcgt attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg
     4081 cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg
     4141 agaaaacccc gtcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg
     4201 gcgcgcacgg caacgcgatc gacttcctga tgaactacga caagctcgag ttcgtcgaaa
     4261 cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc
     4321 ccagccagat cgagcgccat cagaggcaaa cgctttatca gttgatggac ggtctgaata
     4381 cgttttacca acaatcttta caacaacctg ttgccacgtc tgcgcgccag tatctggaaa
     4441 aacgcggatt aagccacgag gttatcgctc gctttgcgat tggttttgcg ccccccggct
     4501 gggacaacgt cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg
     4561 cggggatgtt ggtcactaac gatcagggac gcagttacga tcgtttccgc gaacgggtga
     4621 tgttccccat tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc gtgctgggca
     4681 acgatacccc caaatacctg aactcgccgg aaacagacat tttccataaa ggccgccagc
     4741 tttacggtct ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg
     4801 aaggctatat ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt
     4861 taggtacgtc aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca
     4921 tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg
     4981 cgctgcctta catgacagac ggccgtcagc tacgctttat gtttttgccg gatggcgaag
     5041 accctgacac gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg
     5101 cgatgccact ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc
     5161 ctgacgggcg cgcacgtttg agtacgctgg cactgccatt gatatcgcaa gtgccgggcg
     5221 aaacgctgcg aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca
     5281 gccagcttga acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc
     5341 agctaaaacg cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag
     5401 cgacgttggt cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat
     5461 tcagagaact ggtcaacact tgtctctccc agccaggtct gaccaccggg caacttttag
     5521 agcactatcg tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata
     5581 tagcagataa gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt
     5641 cgctgcttga actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca
     5701 acgaagaacg cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg
     5761 gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa
     5821 atatataagt acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt
     5881 aacggctgaa ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata
     5941 ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga
     6001 gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc
     6061 agatcagatc gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga
     6121 agcaccggat gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga
     6181 agccgccgcg caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt
     6241 acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga
     6301 catcgctaag cgtattgaag acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc
     6361 ggaagcgatc acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct
     6421 gtccgatctg atcactggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc
     6481 cactcacgtc ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga
     6541 agaagatggc gatgacgaca gcgccgatga tgacaacagc atcgacccgg aactggctcg
     6601 cgaaaaattt gcggaactac gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa
     6661 aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca
     6721 gttccgcctg gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga
     6781 ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc
     6841 gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc
     6901 ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac gatgtctctg aagaagtgca
     6961 tcgcgccctg caaaaactgc agcagattga agaaga
//
LOCUS       DAJTUN010000159         6941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTUN010000159 DAJTUN010000000
VERSION     DAJTUN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.159"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(225..1067)
                     /gene="purU"
                     /locus_tag="OO470_003541"
     CDS             complement(225..1067)
                     /gene="purU"
                     /locus_tag="OO470_003541"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6252658.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1117..1575)
                     /locus_tag="OO470_003542"
     CDS             complement(1117..1575)
                     /locus_tag="OO470_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6252659.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1688..2593
                     /gene="rssA"
                     /locus_tag="OO470_003543"
     CDS             1688..2593
                     /gene="rssA"
                     /locus_tag="OO470_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6252660.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2685..3698
                     /gene="rssB"
                     /locus_tag="OO470_003544"
     CDS             2685..3698
                     /gene="rssB"
                     /locus_tag="OO470_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6252661.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3900..4808
                     /gene="galU"
                     /locus_tag="OO470_003545"
     CDS             3900..4808
                     /gene="galU"
                     /locus_tag="OO470_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6252662.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4952..5365)
                     /gene="hns"
                     /locus_tag="OO470_003546"
     CDS             complement(4952..5365)
                     /gene="hns"
                     /locus_tag="OO470_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6252663.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5970..6587
                     /gene="tdk"
                     /locus_tag="OO470_003547"
     CDS             5970..6587
                     /gene="tdk"
                     /locus_tag="OO470_003547"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6252664.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1831 a         1521 c         1697 g         1892 t
ORIGIN      
        1 gaaccttcga agtcgatgac ggcagattta cagtctgctc cctttggccg ctcgggaacc
       61 ccaccacggg gtaatgcttt ttactagcct gctcccttat cgggaagcgg ggcgcatcat
      121 atcaaatgac gtgccgctgt aaagtgttac gttgagaaaa atgaactggt tgcgtaattt
      181 tcatccgtaa cggattaaag gtaaccagtt atttttgctg gcgattaaag aataatcgtc
      241 cgattaccgt aaacaaagac gcgttgcgcc agtactttgt atagcgcacg acttaagacg
      301 tttttctcga cgtcacgacc tgcgcgcatc atatcttcag ctgtgtaggt atgatcgaca
      361 tgaataacgt cctgcatgat gattgggcct tcgtccagat tgtcattcac atagtgagcg
      421 gttgcgccaa taatcttcac accacgttca taggcctggt gataaggacg tgcgccgata
      481 aacgcaggca ggaatgaatg gtgaatattg atgattttat tcgggaagcg tgccacaaat
      541 tccggcgtta atacccgcat atacttcgcc agcaccacat agtcaggttg ataagcatca
      601 atggcgtccg ccatcttttg atcgtgctcg ttgcggctta acccttcatg gcttaccagc
      661 tcaaacggaa tatcaaaacg ctcaaccaga gaacgtaaag tatcgtggtt accaataact
      721 gccgcgattt cgacatccag accgccgtaa ttggctttca tcaacaaatc gccaaggcaa
      781 tgcgcttctt tagtgaccag aatcactatc cggcgacgac cggcaggatt cagctcacgc
      841 acggagcctt ctggcaatgc gctatcgaga tccgccagca gggtggaatc attaaaaatc
      901 ccttccagtt ccgtgcgcat aaaaaagcgc ccggtacggt gatcaacaaa ttcattgttc
      961 tgtacgatat ttaactcgtg cttgtagcaa atattggtaa tacgtgcgat cagacctttt
     1021 tggtccggac aaatagtacg cagaacttta cgttggagtg aatgcattgc tggaaaaacc
     1081 ttgttgagag tgtttgctaa accgtaccgt caaccattat tggccgcagc actttttaaa
     1141 ttttttacct gaaccacaag ggcagggatc gttgcgacca aactgcggac gtgtaccgtc
     1201 aatatagtac cattgaccgt tttctttcaa aaatcgcgaa cgttcaatta ttgcacctgt
     1261 tttgccacct tcggtaaatc gagcaacaaa actgacaaat ccgatattgt cggcgtcctg
     1321 ccaacaatgt tcaaatacgg tcaatcccaa ccattcagta tgcgcaaatc cggtcatcaa
     1381 ttcggcacgt aacgccgctg ctccacaaga gggatgccag gtctttatta aataatcagc
     1441 gtcttgcatc acaaaagcgc agtaacgcga acgcatgaga tgttctggat caggtgcaac
     1501 cttttcacca gacacataag ggtggcaaca taggctatac tcgacagcac taccacaggg
     1561 acaaagctga gacacaaata atctccctgg aaacaataac ggcgtattaa ccgcctgagt
     1621 agcactatgt taaccgagca gtagcgatgt ggctacgatt gcattccagg ggaatcttgc
     1681 gggaataatg agaaagataa aaatagggct ggcgctggga tctggcgcgg cgagaggttg
     1741 gtcgcatatt ggcgttatta atgcgctaaa aaaagtgggt attgaaattg atatcgttgc
     1801 aggatgttca attggttcgc tggtgggcgc ggcctatgca tgcgatcgat tatctgcgct
     1861 ggaagattgg gtaacctctt tcagttattg ggacgtttta cgcctgatgg acctctcctg
     1921 gcagcgcggt gggttactgc gcggcgagcg tgtcttcaat caatatcgcg aaataatgcc
     1981 ggaaacagag atcgaaaatt gttcccgtcg ctttgcggct gttgccacca atttaagtac
     2041 ggggcgtgaa ttatggttta ctgaaggcga tctccatctt gctattcgcg catcatgcag
     2101 tattccagga ctcatggcac ccgttgcaca taacggctac tggctggttg atggggcagt
     2161 agttaaccca attcctattt ctcttacgcg tgcattgggg gctgatattg tgatagcggt
     2221 cgacctgcag cacgatgctc atttgatgca acaagatctg ctctccttta atgtcagtga
     2281 agaaaatagc gagaatggtg attctctacc gtggcatgcg cgtctgaaag aaaggttagg
     2341 cagcataacg acacgtcggg ctgtgacagc gccaacggca acagagatta tgaccacgtc
     2401 tatccaggtg ttggagaacc gccttaaaag gaaccgcatg gcaggtgatc cgcccgatat
     2461 tctgattcaa cctgtttgcc cgcaaatatc tacgcttgat ttccatcgcg cgcacgctgc
     2521 cattgcggcc ggacagctgg cagtggaaag gaaaatggac gaacttttgc cgttggtacg
     2581 caccaacatt tgaccagaat ttttatctac acttaagtta attctgacag gcgcaggtgg
     2641 caatagcatg ccactattga gtaaagccag tcaggggaga gaacatgacg cagccattgg
     2701 tcggaaaaca gattctcatt gttgaagatg agcaggtatt tcgctcgctt ctggattcat
     2761 ggttttcctc attgggagcg acaacggtac tggcggctga tggggtggat gcccttgagt
     2821 tgctgggagg tttcactcca gacctgatga tatgtgatat cgcgatgcca cgaatgaacg
     2881 ggcttaaact gctggagcat atacgtaaca gaggcgacca gaccccagtt ctggtgatat
     2941 ctgccactga aaatatggca gatattgcca aagcgttacg tctgggcgtt gaagatgttt
     3001 tgctgaaacc agttaaagat ctgaatcgct tgcgcgagat ggtttttgcc tgtctctatc
     3061 ccagcatgtt taattcgcgc gttgaggaag aggaaaggct ttttcgcgac tgggatgcaa
     3121 tggttgataa ccctgccgca gcggcgaaat tattacagga actacaaccg ccggttcagc
     3181 aggtgatttc ccattgccgg gttaattacc gtcaattggt tgccgcggac aaacccggcc
     3241 tggtgcttga tattgccgca ctttcggaaa acgatctggc attttattgc cttgatgtca
     3301 cccgagctgg acataatggc gtacttgctg ccttgttatt acgcgcattg tttaacggat
     3361 tattacagga acagcttgca caccaaaatc aacggttgcc agagttgggc gcgttattga
     3421 agcaggtaaa ccatttactt cgtcaggcca atctgccggg gcagtttccg ctattagttg
     3481 gctattatca tcgcgaactg aaaaatctca ttctggtttc tgcgggtctg aatgcgacgt
     3541 taaataccgg cgaacaccag gtgcaaatca gtaatggtgt tccgttaggc actttaggta
     3601 acgcttattt gaatcaattg agccagcgat gcgatgcctg gcaatgccaa atatggggaa
     3661 ccggtggtcg actgcgcttg atgttgtctg cagaatgagc aaacgataac gcgggctaaa
     3721 tttgcattac ctgctaatgt cggctggtgg tactatcgtc gccattcgta taagtaattg
     3781 tcttaattat gctatctcgc ctccttttca gaacttagcc ccttcggggt gctgatatac
     3841 tgggatgcga tacagaaata tgaacacgtt caaaacacga acagtccagg agaatttaaa
     3901 tggctgccat taatacgaaa gtcaaaaaag ccgttatccc cgttgcggga ttaggaacca
     3961 ggatgttgcc ggcgacgaaa gccatcccga aagagatgct gccacttgtc gataagccat
     4021 taattcaata cgtcgtgaat gaatgtattg cggctggcat tactgaaatt gtgctggtta
     4081 cacactcatc taaaaactct attgaaaacc actttgatac cagttttgaa ctggaagcaa
     4141 tgctggaaaa acgtgtaaaa cgtcaactgc ttgatgaagt gcagtctatt tgtccaccgc
     4201 acgtgactat tatgcaagtt cgtcagggtc tggcgaaagg cctgggacac gcggtattgt
     4261 gtgctcaccc ggtagtgggt gatgaaccgg tagctgttat tttgcctgat gttattctgg
     4321 atgaatatga atccgatttg tcacaggata acctggcaga gatgatccgc cgctttgatg
     4381 aaacgggtca tagccagatc atggttgaac cggttgctga tgtgaccgca tatggcgttg
     4441 tggattgcaa aggcgttgaa ttagcgccgg gtgaaagcgt accgatggtt ggtgtggtag
     4501 aaaaaccgaa agcggatgtt gcgccgtcta atctcgctat tgtgggtcgt tacgtactta
     4561 gcgcggatat ttggccgttg ctggcaaaaa cccctccggg agctggtgat gaaattcagc
     4621 tcaccgacgc aattgatatg ctgatcgaaa aagaaacggt ggaagcctat catatgaaag
     4681 ggaagagcca tgactgcggt aataaattag gttacatgca ggccttcgtt gaatacggta
     4741 ttcgtcataa cacccttggc acggaattta aagcctggct tgaagaagag atgggcatta
     4801 agaagtaaca tccgtatcgg tgttatccac gaaacggcgt tgagcaatcg acgccgtttt
     4861 tttatagctt attcttatta aattgtctta aaccggacaa taaaaaatcc cgccgctggc
     4921 gggattttaa gcaagtgcaa tctacaaaag attattgctt gatcaggaaa tcgtcgaggg
     4981 atttaccttg ctcatccatt gcttttttga ttacagctgg agtacggcct tggccagtcc
     5041 aggttttagt ttcgccgttt tcgtcaacgt agctatattt tgccggacgc tgagcacgtt
     5101 tagctttggt gccagattta acggcagcaa ggctattcag cagttcgttc gggtcaatac
     5161 cgtcagcgat cagcatttcg cgatattgct gcagcttacg agtgcgctct tcaacttcag
     5221 cagcagccgc gctttcttct tcgcgacgtt cgttaacaac aacttctaat ttttccagca
     5281 tttcttccag cgtttcaagt gtacattctc ttgcctgcgc acgaagagta cggatgttgt
     5341 tcagaatttt aagtgcttcg ctcattgtag taatctcaaa cttatattgg ggtggtttgt
     5401 tgaggtaata atagagcctt aaattcagtt gtgcaatagc caggaatgta aggaattcaa
     5461 aattgttctt tattttgtgc cgccaataaa tatcttttca taaaattagc cagaaaagac
     5521 gcggcatata gccctattta caccgatgat ttcgcagcac gtgaggttaa aacttcctga
     5581 ttcatgtcac attttatggg gagattatcg taggctgacg acctttcagt cttctgtatt
     5641 agttgtgttt acgagaattc cctattaagc gaatgatgaa aagtagaaca gtcgcaataa
     5701 gagcatggac ttagtattgc actatctcct ggaggtcaac agagggctat tacttgcgca
     5761 acaggttaaa gattgtgaat agttaccagc agtcatttac ccgcttataa caagcgaggc
     5821 agttgtaatg atagctcaga aggattatgc aaggcttcgt aagggagaac gcatataccc
     5881 acttctgtgc atactgttga gctgaaaaac tgacgaatta tgataaactc cagccaactt
     5941 tatttcatat cattgagggc ctgtggctga tggcacagct atatttctac tattccgcaa
     6001 tgaatgcggg taagtctaca gcattgttgc aatcttcata caattaccag gaacgcggca
     6061 tgcgcactgt cgtatatacg gcagaaattg atgatcgctt tggtgccggg aaagtcagtt
     6121 cgcgtatagg tttgtcatcg cctgcaaaat tatttaacca aaattcatca ttatttgatg
     6181 agattcgtgc ggaacatgaa cagcaggcaa ttcattgcgt actggttgat gaatgccagt
     6241 ttttaaccag acaacaagta tatgaattat cggaggttgt cgatcaactc gatatacccg
     6301 tactttgtta tggtttacgt accgattttc gaggtgaatt atttattggc agccaatact
     6361 tactggcatg gtccgacaaa ctggttgaat taaaaaccat ctgtttttgt ggccgtaaag
     6421 caagcatggt gctgcgtctt gatcaagcag gcagacctta taacgaaggt gagcaggtgg
     6481 taattggtgg taatgaacga tacgtttctg tatgccgtaa acactataaa gaggcgttac
     6541 aagtcggctc attaacggct attcaggaaa ggcatcgcca cgattaataa gaatttcttt
     6601 actgacaggg tgagcagggc acttttatcc tgtcagttcg ttttacgcac ttcttccggg
     6661 ctatataccc ttctcggcag ttttttaacg ccgctatacg cctcacaggg ctcttaagca
     6721 ccgacgttga cttgtgacct gtaaagtaca atatccctgt gtttaggcgt tatacatcgt
     6781 cgcaaatatg atgaaggcta atgctgtcgg tttatggaaa agttgctttg ggtaaacaaa
     6841 aaaacggccc cagaaggggt aatgccagtc agttaagcaa ctgactggct ctttttcgga
     6901 gctgtggggt atttccaggg cctctccttt accactctcg g
//
LOCUS       DAJTUN010000160         6897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTUN010000160 DAJTUN010000000
VERSION     DAJTUN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.160"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..721)
                     /locus_tag="OO470_003548"
     CDS             complement(<1..721)
                     /locus_tag="OO470_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6252665.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDE"
     gene            complement(983..2572)
                     /locus_tag="OO470_003549"
     CDS             complement(983..2572)
                     /locus_tag="OO470_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6252666.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            2957..3988
                     /locus_tag="OO470_003550"
                     /pseudo
     CDS             2957..3988
                     /locus_tag="OO470_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(4075..4551)
                     /locus_tag="OO470_003551"
     CDS             complement(4075..4551)
                     /locus_tag="OO470_003551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252667.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(4544..5308)
                     /locus_tag="OO470_003552"
     CDS             complement(4544..5308)
                     /locus_tag="OO470_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6252668.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            5554..6315
                     /locus_tag="OO470_003553"
     CDS             5554..6315
                     /locus_tag="OO470_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6252669.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(6422..6832)
                     /locus_tag="OO470_003554"
     CDS             complement(6422..6832)
                     /locus_tag="OO470_003554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252670.1"
                     /translation="MTKPIVVNNTIQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYA
                     VTFPDDIYAESSTSADAVISGDNNPDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKN
                     TGTGGAYRVVSHMKEQVVEPDSYTIRTQAYIYAE"
BASE COUNT         2140 a         1350 c         1208 g         2199 t
ORIGIN      
        1 cttcatcttc tatattaagc aactggctaa ggaaattttc tccttcagcc atgattccca
       61 aatgtcctgt tgcaaaaagc gcgtacaaaa ctgcaccgac tgagccatcg gtatcgccaa
      121 aataggtcgg cttggtactt gtgggatcaa caggtgaaca attggagaaa taggttctgt
      181 cagcgaactg gggttttata attgtttcga atttaatatc aaatacattg tattgcgata
      241 tttcccagtt ccagatgggt ttgtacgact cagtaactgc aacatcatta gcattatcat
      301 ttactgtcat attattaagt aaataaacaa tgctgcacca attaatatcg gtattatcaa
      361 gagggcaacc gtaatcttta ctattaattg tcacaataaa ctgataacag ttattcctac
      421 gtagaatatg agcgcgcatg cccgatagca tactatattt ttctgaatga ttcgtttgta
      481 tggtaaatga aattttgtca tcttgaatag attgttggaa acgttctatt tgccaatcat
      541 caattttttc cacaacttca ccgagcattg tcctggcaat acttgatata tcatcaccta
      601 taccatttgc aatataccca aacagttctt tagtgaagtt ggtaacatgc tcttgcacca
      661 tatcatttgt tgcgattgtc gttgtactgc cattagtgcc tgccacagtt ccaattttca
      721 tcttttacct catattattc accgcgagga atacatagtt aacattttat cttatgacat
      781 acacctcata ttcgatgggg aaataattat attttgcaca aatcattcag ccttaaagca
      841 taatatgaaa tttcttatct catagcagag aaagtcccta aagattgtag aggcgtcatc
      901 agtacatacc tctacaatcg attaagaaaa cctatgaaat actggcaaaa ttaaaaagag
      961 ataccacagc actcatgaag ttttattgag gttgaaaaca actaagccct gggacacctt
     1021 ttttatcacc acgttcgtta ggtgctatct tccaagaatt tagccccatg gtataattat
     1081 caattttttg atgctggtct acccctaatc cataatggcc aaatttctgt ttagtttttt
     1141 tgagcgtctg tccacatttg aagatatctt caggttttgg tctgaaagca gtagtgttat
     1201 taaaataatt ttcatataca ggtgtgggtg ctatagcatt gcttctaatt agatggagaa
     1261 gatgatcatc catgtgttgt ttatgttttt tattacatat atttgctaat tcctcaggat
     1321 caattgggtc tttcatctga ttaaatggaa cctgcaaagc accgagacaa taacattgat
     1381 tttcttcatt tcttattatt ccgtgatgac tggttaaaac tttagtttct gagttataat
     1441 tacaaacatc gagcgtttta attaagttat acgtatcacc ggcagagagt ttataatttt
     1501 ctagtcgtgc agtatagtta ccattaaaat tgatttcatg atttccagcc agaacgatga
     1561 catttttatt aatacggcta tctttattac cctccatatt tcgcatagaa tttagcaaag
     1621 taaggatata tttatcacca aaaatagtac taaatctatc gcctgtatga tccccaagga
     1681 agatacaagg ggttatcgca tcgtccttta aaaaaacatg ttgatgcaac tcattcaata
     1741 tcatgtctct cgtatttaat aaattgtaga gcaatttatt atttttatgt atgtatgcag
     1801 atgaagccat gtcttcgtgt tttaataatt cacataataa actccaaccc ttttccctga
     1861 tgcctatatg ccctgaaacg agcaatgaat aaagtacagc gcctactctt ccatctgtat
     1921 cgccaaaata agccgtgttg gcattttgtg ggttaatcac accacattgt ttataaaagt
     1981 ctattttaga aaacacagat ttaacatagc tatcaaactt atccttagga aacgtctctt
     2041 tagagacatc ccaacgccag tcagctttaa aattaccgct aagaagagta aagaaatttt
     2101 caggtatgat agaaagccca ttaactttat aaattatgtc tgatttatct ccggagaaat
     2161 taataaaata ataaccatcc ttacactcaa tcttaactgt aatgggagac gataatttta
     2221 tcacatggtt attgtctggt ataattgtaa agataaacgt gttagtagta ttatcggcaa
     2281 aactattata ttcttgaata tctatagatg gctcactatt gtaaaaccat attttctcca
     2341 tctgaagact atgttcattt accctccagt gaccatgttg caggaaatag atgatatgca
     2401 tgccaaaatc gttatctatt tgcgaatcat tatccagctt atcaatttca actatctttg
     2461 aacgatgtgt tttatcaacc ttgctatggt gttgatgatt attaacttta tctgaatgct
     2521 tatctttatg gtttgaaggg atgtacttaa atatattgtc aattgctttc ataaataacc
     2581 cgcgatattt ttgacctgtt agtaaaaaat aaatgataca tgtctgaaag acatatttac
     2641 gacgatataa aacaataata cttatatgtt cgctgatttt aagcaccgag actatccggt
     2701 cttttactaa ataatggaaa acaatccatc cctggaagac aattgtaaaa aaaaacaaca
     2761 gaataagaat taatcttagg ataaatattt atttatcatg gccttttgac cgctaaaaaa
     2821 tggcactcgg cacagtaaac cccgctatag ttatgctatg catttgtctt taatgatatc
     2881 tcgtcgttat attaacaggc tgaaattatc attaatgcac atttcaatat tagcagggat
     2941 acctgaggag tttaaagtga acatatatat cgggtagctt ttcaaattaa tccctttgat
     3001 tataggctta atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag ggcaacgtca
     3061 cgggtcatgt gctgatttct ctggcggcca tatgcctggc attattcact accgcattta
     3121 ttatcatttc gcaactcacg cgcggcgtta atacgtttta caatacattg ttccccatta
     3181 ttggctatgc ggggtcaatt atcaccatga tatggggttg ggcactgtta gcaggtaatg
     3241 atgtgatggc agacgagttt gttgccggcc atgttatttt tggcgttggt atgattgccg
     3301 catgtgtatc gacggtggca gcgtcgtccg gtcactttct gctcattccc aaaaatgcag
     3361 cggggagcaa gagcgacgga acaccggtac aggcttattc ttcattaatc ggtaattgcc
     3421 tcattgccgt tcccgtttta ctcaccctgc tcggtttcat ttggtctatt acgctgttac
     3481 atagtgccga cataactccg cattatgtcg ccttgggtta accgcaatct gtgcctgtct
     3541 aattggccta gtggccacaa tcgtccatca aacacgtaat acgttctcat ccaaagagca
     3601 ctggctgtgg tgttattggg ttatttttct cggctcagtc acggtgctgc aggggatata
     3661 cgtcttagtc agttccgatg caagcgcccg actggctccc ggcattattc tcatttgcct
     3721 cggaatgatc tgttacagca tattctcaaa agtctggcta ctggcactgg tctggagacg
     3781 tacctgttcg ttagccaaca gaataccgat gataccggta tttacctgtc tgttttgctt
     3841 gtttttgtca tcattcctcg cggaaatcgc acaaactgag ctggactact ttattccatc
     3901 tcgggttctg gttggattag gcgcagtatg ttttacattg ttctctattg tttcaatatt
     3961 agaggccggt tctgcgaaaa aaaattaaaa tttacataat cccagcgtta ttcatatctt
     4021 aacgctggtt ttttacgaac ttagtgaaat aatgtagaat ataaaattat aattttatat
     4081 tggagcacat gtgttaatga tcaaccccgg gatactaaaa atatagacat tattaaactt
     4141 gcttagcctc gcattgcctt tacctgttaa agatgcatcg agaaaattga gtctgaagtt
     4201 ctcatccttt atctgatttt ccagtcccga aactttggcc ttagcattac ctgagatccg
     4261 tccgttacta tctattttgt aggtaaaaaa atacgaatgg gaaaatttcc tcggtgtatc
     4321 ttcatcagtt aagccaacaa tcgtaaaact accgctacca tcttttttta aaatgactga
     4381 aaggttagca gagtagtata aagaatgatc tgttttagca tttcttgtta acgtcgacgt
     4441 acattcaaga gacgttgtgc taccatcacg agccttccag agagcaaaaa aacacacctg
     4501 tagcaatgtg aaaacaagta ttaatataaa atacattttc ttattagtca tataatagca
     4561 cctctgaaca aattgcataa gtttcgtcat tagaggattt tatacatctt gataaatacc
     4621 gctgcccttc cccaagcaaa taatgcatac tatttacatc atatataaaa tttgcacctg
     4681 gcacacaggg taaatgcttt ttcgaaattt cagcggcctc tatttgagat ttagcgaggc
     4741 caatctcgtt aagattctct gaaagatata aatcacaggt gtcaatctta cctgatttaa
     4801 ccaatggata atccgttata ctgtaggtat ctttaaaagc cacggcaaat atttcaataa
     4861 taacaattac taccacaagc gaaatcaaaa tgcgaaaaag aggaatactc cttttgcgct
     4921 ctactttttc aacaacatct tcttgtaaat gagaatcatc ataagattgt ttacttattg
     4981 ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa
     5041 caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg
     5101 acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc
     5161 caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctggc aaatttatct
     5221 catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat
     5281 tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat
     5341 tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa
     5401 agtcatataa aacttatatc cacagaataa aaaatcaaac atgtcagttt tatcaatata
     5461 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
     5521 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
     5581 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
     5641 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
     5701 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
     5761 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
     5821 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
     5881 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
     5941 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
     6001 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
     6061 atagaaaacg ctatcaatga ttcttttaac agcaagccta aaaaaataag atttttaaca
     6121 gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
     6181 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
     6241 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
     6301 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
     6361 ttttataagt taaaaaaggc gggatatcaa accccgccat gaaattaact aatcatctgc
     6421 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
     6481 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
     6541 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
     6601 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
     6661 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
     6721 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
     6781 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
     6841 tagctgacgc ttcagtaccc tagcttacta aagttcccgc ctgagaagca aaagctg
//
LOCUS       DAJTUN010000161         6822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTUN010000161 DAJTUN010000000
VERSION     DAJTUN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.162"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..327)
                     /locus_tag="OO470_003555"
     CDS             complement(<1..327)
                     /locus_tag="OO470_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6252671.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYV"
     gene            509..1933
                     /gene="sbcB"
                     /locus_tag="OO470_003556"
     CDS             509..1933
                     /gene="sbcB"
                     /locus_tag="OO470_003556"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6252672.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1976..2203)
                     /locus_tag="OO470_003557"
     CDS             complement(1976..2203)
                     /locus_tag="OO470_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6252673.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2217..3275)
                     /gene="yeeE"
                     /locus_tag="OO470_003558"
     CDS             complement(2217..3275)
                     /gene="yeeE"
                     /locus_tag="OO470_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6252674.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3454..4812)
                     /gene="plaP"
                     /locus_tag="OO470_003559"
     CDS             complement(3454..4812)
                     /gene="plaP"
                     /locus_tag="OO470_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6252675.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4802..4864)
                     /gene="yoeI"
                     /locus_tag="OO470_003560"
     CDS             complement(4802..4864)
                     /gene="yoeI"
                     /locus_tag="OO470_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6252676.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5025..6008)
                     /locus_tag="OO470_003561"
     CDS             complement(5025..6008)
                     /locus_tag="OO470_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6252677.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLSDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6054..>6822)
                     /locus_tag="OO470_003562"
     CDS             complement(6054..>6822)
                     /locus_tag="OO470_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6252678.1"
                     /translation="LSARGWQVTGSKTTQDGVEAARMSGIDSYLLRMEPELVCDSDDL
                     DALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAHRIPRIIFTSSTSVYGDAQG
                     TVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLAGLVGPGRHPGRFFAGKTAP
                     DGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHPARNVFYPQMARLLGLQPPQ
                     FRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
BASE COUNT         1845 a         1780 c         1625 g         1572 t
ORIGIN      
        1 gacgtagttg tcgaaccagt agtcgagaca ctgggcgacc gtcatggagt tctccacctt
       61 ctcttcaaaa taggtgcgtg gatccgttcc tgagaaatag agctttcgca agtcagcagt
      121 gatctgtctg gcatccttca aagacaggga tgggtatcga ccaagcccaa gtcgattagg
      181 cttgccatgc caacgatagc ggtactggaa ctggatgacc cccttcggtg aaattcgtac
      241 gctgaggcca tcggcatcag ccacttcttg tgggcccgaa tatggtttac cataaatagt
      301 acgcagtttt gtgtcgctta tagccataaa taatatttgg tacgctcaga aataatagtt
      361 tggcacacat ctggtacaca atatcacatg gacaacagcg cacagtaaac aactatattt
      421 gacgttcgta gacatactca ggtgaaaaaa gggttgcttt tcgagttata acagacagtt
      481 aattaactac agtggacaat gctagacaat gacagacaca gataaacaac ctaccttcct
      541 ctttcacgat tacgaaacct ttggcacgca ccccgcgtta gatcgccctg cacagttcgc
      601 agccattcgc accgatgacg aattcaatgt catcggcgaa cccgaagtct tttactgcaa
      661 gcccgcggat gactatttac cccggcctgg agcagtatta attaccggta ttaccccgca
      721 ggaggcacgg gcgaaaggag aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt
      781 taccgtaccg aagacctgta ttctgggcta caacaatgtg cgtttcgacg acgaagtcac
      841 acgcaacgtt ttttatcgta atttctacga tccttacgcc tggagctggc agcatgataa
      901 ctcgcgctgg gatttactgg atgttatgcg tgcctgttat gccctgcgcc cggaaggaat
      961 aaactggcct gaaaatgatg acggtctacc gagctttcgc cttgagcatt taaccaaagc
     1021 gaatggtatt gaacatagca acgcccacga tgcgatggct gatgtgtacg ccactattgc
     1081 gatggcgaaa ctggtaaaaa cgcgtcagcc acgcctgttt gattatctct ttacccatcg
     1141 taataaacac aaactgatgg cgttgattga tgttccgcag atgaaacccc tggctcacgt
     1201 ttccggaatg tttggggcat ggcgtggcaa taccagctgg gtggcaccgc tggcgtggca
     1261 tcctgaaaat cgcaatgccg taattatggc ggatttggca ggagacattt cgccattact
     1321 ggagctggat atcgacacat tgcgcgagcg tttatatacc gcaaaagccg atcttggcga
     1381 taacgccgcc gttccggtta agctggtgca tatcaataaa tgtccggtac tggcccaggc
     1441 gaatacgcta cgcccggaag atgccgaccg actgggaatt aatcgtcagc attgcctcga
     1501 taacctaaaa attctgcgtg aaaatccgca agtgcgtgaa aaagtggtag cgatattcgc
     1561 ggaagccgaa ccgtttacac cttcagataa tgtggatgca cagctttata atggcttttt
     1621 cagtgacgcc gatcgtgcag caatgaaaat tgtgctggaa accgagccgc gtaatttacc
     1681 ggcactggat atcacttttg ttgataaacg aattgaaaag ttgttgttca attatcgggc
     1741 acgcaacttc ccggggacgc tggattatgc cgagcagcaa cgctggctgg agcaccgcca
     1801 ccaggtcttc acgccagagt ttttgcaggg ttatgctgat gaattgcaga tgctggtaca
     1861 acaatatgcc gatgacaaag agaaagtggc gctgttaaaa gcactttggc agtacgcgga
     1921 agagatcgtc taatttgaag ccgccgccgc tggtacggcg gaggacaatg ttgagtcagg
     1981 ctttttgaac ggtgatgctc cacgctgcat cgccaatctg ctgataatcg gtgatggcat
     2041 gtccttcttc tgccgcccac tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa
     2101 ttaccaactc atcgccgctg gccatttctg ccagtgctgc ttttgcttct atgagtggaa
     2161 acggacagac ctgcgtcacc acatccagct ttttaatcgc catctttaca ccttacttaa
     2221 tttgccgcag cagttgccag tcgcgcctta cgctgcggtc gaacatacac aagccaggac
     2281 gcagtccaga ctccgagaat cataaataca aggccaatcc agccctgcca ggtcatcatt
     2341 gcagtcataa ccagtccatt accgatagag caaccacctg caatactggc accgaagccc
     2401 atcagtacac cacctaaccc gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg
     2461 aactcacggc tcgctttggc tgcaataaat gcctccacga agatccctaa caccaggaaa
     2521 acgccccagt taatgtattt cacgtcaccc gcgaccagaa attgaagaat attggccgtt
     2581 ggggaagtga ctcctaaccc aaacatgcgt ccggttgctt cactcagggg ccaggctaaa
     2641 agcgcaatca aaccgatgag tacagcagtg acaaagggat gccagcgttt ttcaaacaga
     2701 atatgagcga tcccggttcg gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc
     2761 aactctttca tcaccaccca gagcgttatc accagcaaaa cggtaaccaa cggccacaca
     2821 gacaaattaa aagtctcagc aatagagtta tgttcagtag tgtagtgctg caaggtttga
     2881 tttaaaccac tggcgtgtgg agaacgcatc accgcactca tcaccatata agtgaaaagc
     2941 gcgatccaac tgccgatcaa tccctcaccc gcgcgatacc aggtcccggt ggcacatcca
     3001 cccgccagaa caattcccag cccgaaaaga tacccaccta taacagtacc aagccacggg
     3061 aacgccccgg cttcgtaagt cagtaggccc gcctgaatta acgcaaaaac ccccacgctt
     3121 tgtgccgaaa ttgcaatcag caaggcgtaa aacatgcgat tatttttcgc gatatacata
     3181 tcgcgaaaac caccagtcag gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca
     3241 ccacaaatta gcccgcttaa tatcattgaa aacataaatt aattaaccag atgaatgtta
     3301 atggggaaat ttttcatgac tggtggaatg cagaccaata accaaattct ctaaattaga
     3361 acaaatggtt attaatgagg atttcgataa aaaagaataa aaaaaccgga gccaaatgtt
     3421 cattcgactc cggtttatta ttagaaaaga tgattatgct acgtcttcgt actgcggaac
     3481 cggattacgg aagctcttgg tgacgcaagc caggtaaatc agaccgatag cagcccagat
     3541 cagacccaga accattgagc tttcttccag gttaacccac agcgcaccaa cggtcagcgc
     3601 accacacatc ggcaggaaca gatactggaa gtgatctttc agcgtcttgt tacgcttctc
     3661 acggatccag aactgcgaga tgaccgacag gttaacaaag gtgaacgcca ccagcgcacc
     3721 aaagttaatc agcgccgttg ccattaccag gtcgaagttg attgccagca gggcaatcgc
     3781 gccaaccagg atgatgttca tcgccggagt acgccatttc gggtgtacat aaccgaagaa
     3841 gcttttcggg aacacgccgt cacgacccat tacgtacatc agacgcgcta cgcctgcgtg
     3901 cgccgccata ccggacgcca gtacggtaat ggtggagaag atcagcgcgc caacctggaa
     3961 cgctttaccc gcaacgtaca gcatgatttc aggctgtgac gcatccggat ctttaaagcg
     4021 agagatatcc gggaagtaca gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc
     4081 aatcagcgcg gtcaggaaaa tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc
     4141 cgacagattg ctgatgccgt caaagccggt aaaggagaag cacaggattg tcgccccggt
     4201 aatcatcggg ataacatgcg catcgccaga ccagaacgga cgcgtgctcg ccagcgtacc
     4261 agcgccttca ccttcaaata cgccataaac aaccatgccc agaatcaccg cgatcagcac
     4321 tacctgcaac acgacgatta cagtgttgac gttcgctacg gatttcagac tacgcaggtt
     4381 aaaggcggtc ataaaggcca ccagcgccac cacaaacatc cacgatggaa tggaaggcac
     4441 cagagcttca aaatagattt tcgccagcag aatgttgatc atcggcgcga acagatagtc
     4501 gagcagagaa gaccaaccca ccataaagcc gacagtcggg ctaatggatt tctgggcgta
     4561 agtgtatgca gagccagcag aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt
     4621 aaacaggatc gcaatcaatg cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc
     4681 tgaaacgata ccaaatgtat caaacagcgt catcggctgc atataggcta gacccatcat
     4741 tacaaccgga actaacgtaa gcgttttacg caattccacg cgagaggtgt ttggagtaac
     4801 gttatgcgac atggttattc tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc
     4861 ccattttttt ggattcctca gcgacaacaa ctgtcgattt ttagtaaata tctatccggt
     4921 acgaagcccg gcctcttggt atgaatgatt ggtttgaagc aaaaaataac cgacgctaat
     4981 taaacgtcgg tttttaatca ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa
     5041 tgacaagaga atgagaggct tgtcataata atttttcttt gaatggctga ttttccgtca
     5101 tcagattttg cataaacacc ggcgatcgca ctatttgcta aaatttcatc tcgccaccag
     5161 ccgctggcaa gccctgcggt ctgttcattc cagccaatcc agacaggttc aaagagagtt
     5221 tgggcgacca ctttcttctc gattaacgcg ccactatcga gaaaacgttg cgccagatac
     5281 cgtggtaaat agccgcagcc aaggccgcta atttgcagct ccagtttggt tttaaaatcg
     5341 aaaacggtaa tggcttcttg ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc
     5401 gcagtgtccc ccaccacaat cgcccgatat cgtttaatga tacgacggtt taacggttct
     5461 tcttcctggg ctaacggatg atgaggcgcg accgcaaaga cttgttcaag atcgctcaac
     5521 cgcgagaaac caaactcact ggaagatggt ggctcatgca tcgcgccaac gataatatcc
     5581 gctcgcccct gagtcagggc atcccaggaa ccaccgagta cgccattgat aaacttcagg
     5641 cgcgtcacgc tatgatgttg ataaaaagct tcaatgagag gcgcaagcag agaaaaagga
     5701 aacgtgtcgt ccacgccaat caccagctcg ttttcccagc cttcatgcag tttgatcgcc
     5761 tgcttttcca gttctcgcac ggtatgcaga acttcccgcc ctttctctaa taacattttt
     5821 ccggtgcggg tgaatttagc gcggtgtccg ctacgatcaa gcaattgaat atttaggtcg
     5881 ctttccagct tgtgaacggt gtaactcaga gcggacggcg tcttgtagag tttggctgac
     5941 gccgccgcaa aactgccctc tttttctatg gcatcgagga tcatcaacac atccagcagt
     6001 ggtttcatac acgccccctt gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc
     6061 aacggcatta ccagcggatc gggatactgg tattcaaatc ccagttcatt acaaatccga
     6121 ctgccatcaa taatcttacc tttgccgctg tccagactat ttctgaactg cggaggttgc
     6181 agccccagta aacgggccat ctgcggatag aaaacattac gcgcagggtg agctggcgca
     6241 catatattat agatgtgtcc gcctttaggt gcctgtaaca acagagtgat agcgccaata
     6301 acatcttcta aatggactaa attaacacca tgttcaccat caggggcggt ttttccggca
     6361 aagaagcgtc ccggatgacg tcccggtccg accaggcccg caagacgcag aatatcgacc
     6421 gaagtaccgg gtaaattgtg cagccagtct tcgagttctt ctaatactcg tccactgttg
     6481 gttaccggat tacgcggggt agtttcttta accgtgcctt gcgcgtcgcc atagacagat
     6541 gttgagctgg taaaaataat tcggggaata cgatgggcca gcgcgctatc cactaactct
     6601 tgtaccgctt gtaaatagaa ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg
     6661 accagcgcat cggcatccat aagggcatcc agatcgtcag aatcgcaaac taactcaggc
     6721 tccatgcgaa gcagatagct atcaatgcca ctcattcggg ccgcttcgac accatcttgt
     6781 gtggttttac tcccggtgac ttgccagcct cgcgctgaaa gt
//
LOCUS       DAJTUN010000162         6561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTUN010000162 DAJTUN010000000
VERSION     DAJTUN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.163"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..302)
                     /locus_tag="OO470_003563"
     CDS             complement(<1..302)
                     /locus_tag="OO470_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR6252679.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTV"
     gene            complement(299..1177)
                     /gene="xdhB"
                     /locus_tag="OO470_003564"
     CDS             complement(299..1177)
                     /gene="xdhB"
                     /locus_tag="OO470_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6252680.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1188..3485)
                     /gene="xdhA"
                     /locus_tag="OO470_003565"
     CDS             complement(1188..3485)
                     /gene="xdhA"
                     /locus_tag="OO470_003565"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6252681.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            3900..4655
                     /locus_tag="OO470_003566"
     CDS             3900..4655
                     /locus_tag="OO470_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6252682.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4734..4807
                     /locus_tag="OO470_003567"
     tRNA            4734..4807
                     /locus_tag="OO470_003567"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4766..4768,aa:Gly,seq:ccc)
     gene            4922..6526
                     /locus_tag="OO470_003568"
     CDS             4922..6526
                     /locus_tag="OO470_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6252683.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT         1637 a         1608 c         1728 g         1588 t
ORIGIN      
        1 actgtgccgg atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca
       61 ccttccagcg tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct
      121 attgctgtgc cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt
      181 ttgacactta gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg
      241 tgaagctgaa aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc
      301 attgcaattt tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct
      361 ggatgagatg cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt
      421 gcaggacaga ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag
      481 tctgttcggc atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta
      541 attcgctgaa attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat
      601 ccattgcgtc gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg
      661 gcggaaaatg aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg
      721 tgtgaaagcc attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg
      781 cgtcataaat tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc
      841 caccgtaggt agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata
      901 acgccgggag atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag
      961 agccgatacg tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat
     1021 gaatatcaac aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag
     1081 tgccaccggc gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa
     1141 gggttgctgc gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct
     1201 gccagatgga actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca
     1261 ccggtagcca tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca
     1321 cccagtgact tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc
     1381 agttgtggca gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc
     1441 acgccgcttt tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc
     1501 attccgccgt gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga
     1561 acgttgagga tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca
     1621 aaggtacagc caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc
     1681 tgcccgccgc gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac
     1741 ggctcttccg gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct
     1801 gactgatgta gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca
     1861 ctgcgcagcg caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac
     1921 ggcgtaacgt cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg
     1981 gtttctgcca ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg
     2041 ctttgcacgt tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg
     2101 ccgacaggcc aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa
     2161 ttgccttgct ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct
     2221 ttttcaagac actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca
     2281 tctccttcgc gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc
     2341 gcggcgtcat caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc
     2401 atcgcaccag ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa
     2461 cgaggataaa ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat
     2521 gcataagcgc cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg
     2581 ttcacgccca tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac
     2641 tcttcacggc taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc
     2701 gccattggct cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg
     2761 atgactcgta cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg
     2821 tgcgggatct gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc
     2881 gatgttacgc tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc
     2941 tggtagtcgg cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc
     3001 agtaaattgc cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg
     3061 gtgataacgg gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg
     3121 agttcatcgc gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt
     3181 agcagtgcgc gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct
     3241 gtagcgaatg gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa
     3301 cttctggctt gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca
     3361 tatttcgcgt aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg
     3421 accttagcaa tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct
     3481 tccatagaaa tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca
     3541 ggcaagattt acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt
     3601 taaaaaaaca cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa
     3661 tatgagtggc gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat
     3721 agggtaaaaa gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc
     3781 ctttgccaaa tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt
     3841 gggtatcaag aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt
     3901 tgagtgcggg acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac
     3961 tgctattggc gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg
     4021 tttacaccgt gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg
     4081 taaaagagct ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga
     4141 aactaaaact gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa
     4201 cgactaaaac cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag
     4261 tagggcaacg ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag
     4321 cagatggcgg caataaaggg attgatatct cagctccacg gggtacacct atttacgccg
     4381 cgggtgcagg aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca
     4441 tgattaaaca cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa
     4501 ataatgggca aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg
     4561 catctgttcg cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt
     4621 acttgccgcc gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag
     4681 cgcggcactt gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg
     4741 tagttcaatg gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc
     4801 cgctccatga cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa
     4861 gcctccttgc agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca
     4921 tatgattcag ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa
     4981 cggcgtctac tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt
     5041 atcattacgg accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc
     5101 actcaaggca atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct
     5161 taaggacatc gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat
     5221 gagggatatc taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc
     5281 tagtgagccg ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa
     5341 agcctgtatg aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg
     5401 ttttaatgat attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc
     5461 tccagaagta aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata
     5521 cgaagcggag aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca
     5581 cgctgcgtta atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga
     5641 catgcttaag gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa
     5701 ggatgcccca cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt
     5761 taccatcaac aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa
     5821 tgacttgatt aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc
     5881 ctcaggggcc cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa
     5941 ggcatactct cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc
     6001 tatcactggg gcgagactca atgaaatagc ccaactacag gtcaaagacg tcaggaccac
     6061 tgaggcggga accgtttata ttcatatcaa tgaagacgat agtagtctac ccggtaagag
     6121 catcaagaac gctcatagtg accgctgtgt gcctctggtc gatggggcct atggttttat
     6181 attggctgac tttatggcgc ttgtagagac ccgtagaggc gctgacggtg atgacgctat
     6241 ggtgttcgat ggtctgcgtc tcatgaagaa cggttacggt gagcaggtga gtaaatggtt
     6301 taatcggacg ttgttgccta aagtcctcgt agaccgtagc ggcttagcgt ttcactcgtt
     6361 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttggcgt atgcacaggc
     6421 gatcatgggg catagttcag ggtctattac gtacgaccgt tatgctaaag aggtcgaggt
     6481 tgataggctg gtcaatgtga tggctgatgt ttataaggag acctagagga gaccatagtc
     6541 taggatgaca cttactacag a
//
LOCUS       DAJTUN010000163         6509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTUN010000163 DAJTUN010000000
VERSION     DAJTUN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.164"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            50..2482
                     /locus_tag="OO470_003569"
     CDS             50..2482
                     /locus_tag="OO470_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6252684.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2549..4018
                     /locus_tag="OO470_003570"
     CDS             2549..4018
                     /locus_tag="OO470_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6252685.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4018..5679
                     /locus_tag="OO470_003571"
     CDS             4018..5679
                     /locus_tag="OO470_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6252686.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDTAIN"
     gene            5900..6241
                     /gene="symE"
                     /locus_tag="OO470_003572"
     CDS             5900..6241
                     /gene="symE"
                     /locus_tag="OO470_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6252687.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6288..>6509)
                     /locus_tag="OO470_003573"
     CDS             complement(6288..>6509)
                     /locus_tag="OO470_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252688.1"
                     /translation="FVDVVPQKSRLRLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVE
                     IGFSDLAQLPYIMGLIRQAFEKQMESALV"
BASE COUNT         1921 a         1552 c         1505 g         1531 t
ORIGIN      
        1 ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca tggcggaact
       61 gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc cagccattct
      121 caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc gggacggtaa
      181 agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg acatcgtgct
      241 gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca aacatgaaat
      301 tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc cttttgtttt
      361 tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag gtgaatgcct
      421 tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc aaaaattctg
      481 cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact attacgacga
      541 tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa ccattgaagc
      601 cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag ggaaaaccta
      661 taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac gcattttgtt
      721 ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc agccatttgg
      781 tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa ttcacctcgc
      841 gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac aagtcgcacc
      901 agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat ccgaagacag
      961 cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct taaccgccac
     1021 gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg tttacgtcta
     1081 ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg tccgtgttga
     1141 tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact taaacggcga
     1201 agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg tgatcgacga
     1261 acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca atccgatgga
     1321 taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc gcgccctggt
     1381 taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa tcaccggcga
     1441 tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac cgtacccggt
     1501 tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct gcaaactggt
     1561 agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg gtcgtggtac
     1621 acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta aaaaagccac
     1681 cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg ataccacacc
     1741 agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa tcagcgaaca
     1801 tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc aggttaccga
     1861 taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca agtttcacgt
     1921 caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg ccgacggtaa
     1981 actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag aatatgcctc
     2041 gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga tcattcacga
     2101 gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta aagatctcga
     2161 cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc gcaaagagcg
     2221 cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag cgcaagccgt
     2281 gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg aaagtattca
     2341 ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta ttaaaaccgg
     2401 atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg aaatctacca
     2461 attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca tcatgttgca
     2521 gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca aatcattaca
     2581 agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg gtcagctctc
     2641 ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac tggagcagga
     2701 taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg ccaacgcgca
     2761 gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc cggcgttgaa
     2821 aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc aggcgttcag
     2881 cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca acaagctgaa
     2941 cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt acgaacagat
     3001 ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac gcgccgtcac
     3061 tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg acccggcctg
     3121 cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat acgtgaagag
     3181 cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa aacagcttcc
     3241 gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg tacaaattcg
     3301 tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg atgtcattgt
     3361 taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact ttccggcaga
     3421 gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag tactggcgaa
     3481 aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag gtgttaaaac
     3541 caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc gtttaccgaa
     3601 tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta ccaaaggtca
     3661 gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga aaaactacag
     3721 caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt ggggtaacga
     3781 ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca gcattgatga
     3841 cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg aaaccgtcag
     3901 ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga tccagacgct
     3961 gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg ttaactaatg
     4021 agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt gcaaacccgt
     4081 tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa gaaattgcgt
     4141 gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc gaatgatgaa
     4201 ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt gaagcaaggc
     4261 aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc gtttgaattg
     4321 ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc tgagtcatct
     4381 agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat cgagcaaagc
     4441 caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt aaataatcca
     4501 gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga ttttgttgtc
     4561 ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt ctttttttgg
     4621 cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa agtattaaat
     4681 agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga aaaagttagc
     4741 tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct ggacgcacat
     4801 cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac cgcggaactg
     4861 gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac cgaagccagc
     4921 gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact tgtgccgcag
     4981 gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga aaaagctcaa
     5041 ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag cgatgaggaa
     5101 aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat agttaatatt
     5161 aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc acctgatatt
     5221 attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc aggagtcaaa
     5281 gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg cccgtcatta
     5341 tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta tccattagac
     5401 tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt tcttatgcaa
     5461 gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag tttttacaat
     5521 attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa gataaattct
     5581 attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa aacccaactg
     5641 catcttgcag acgcactcac tgacacagcg ataaactaat tccctcgccc tcacaaaacc
     5701 aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt cacgctaatt
     5761 ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga tgatgtatcc
     5821 aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc acaatcacta
     5881 tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt tagaagcaga
     5941 agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt acccggatta
     6001 cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg gttttgccac
     6061 cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg cccaacccgc
     6121 cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc tgtcggcgcg
     6181 taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga aagtcgcctg
     6241 atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta taccaacgcg
     6301 ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg aagttgtgcg
     6361 aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt tgtcacatct
     6421 ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga caatcgcagt
     6481 cggctttttt gcggcacaac atcaacaaa
//
LOCUS       DAJTUN010000164         6479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTUN010000164 DAJTUN010000000
VERSION     DAJTUN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.165"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..140)
                     /locus_tag="OO470_003574"
     CDS             complement(<1..140)
                     /locus_tag="OO470_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252689.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVS"
     gene            complement(152..655)
                     /locus_tag="OO470_003575"
     CDS             complement(152..655)
                     /locus_tag="OO470_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252690.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(660..1880)
                     /locus_tag="OO470_003576"
     CDS             complement(660..1880)
                     /locus_tag="OO470_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6252691.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(1895..2632)
                     /locus_tag="OO470_003577"
     CDS             complement(1895..2632)
                     /locus_tag="OO470_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6252692.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(2520..3986)
                     /locus_tag="OO470_003578"
     CDS             complement(2520..3986)
                     /locus_tag="OO470_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6252693.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(3986..5608)
                     /locus_tag="OO470_003579"
     CDS             complement(3986..5608)
                     /locus_tag="OO470_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HCR6252694.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(5611..6183)
                     /locus_tag="OO470_003580"
     CDS             complement(5611..6183)
                     /locus_tag="OO470_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6252695.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(6167..>6479)
                     /locus_tag="OO470_003581"
     CDS             complement(6167..>6479)
                     /locus_tag="OO470_003581"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6252696.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         1369 a         1841 c         1646 g         1623 t
ORIGIN      
        1 gagacatctt cacgcaactg aatatcgcgg gtccaggtgt accccgccag tggcaggttc
       61 agagtctggt cgagtcgctc cagctctccg atgagaaagg caccagagct gtcaacggtt
      121 gcctgatcaa aagtaatcat tcgtctgttc cttaaatctt ccaggaaatt tctgcattgc
      181 cgttagcatc accggcacct gtgaattcag cgttggtcag caccacattt ttgccactga
      241 ctgacgtgga catgaatcca cccagcggca ctttgatgga ttcatcagtg gagacgacaa
      301 cgtataccgg gtcgcctttt ttgatggtgc tggcatcaaa atcagaaccg agattaacgg
      361 tcacgtagcc acgcttcatg gcgtcgcccg ggaagttctt gcctgttccc acctggcgaa
      421 ccatgtccgg ctgcgaagtg gtcggataag ggcgcacgta gatccccttc accttgtctg
      481 cggtatcgcc atctgccagc ggcacgaaaa aaccgtcagc atcgtattta ccagccagcc
      541 cataggcagc aaaggcgtta tcggatttaa ggaccaccgg ttcgacggtt aagtcctgcg
      601 ggcgagagat agccccggca atgccaacag gcatccggta cagatatgca gtcattggat
      661 tatcctttgc ggttagacca gaattcagcg ttttgtttgt tcagggaagc gatgctggtc
      721 atgcccatgt ttggacgttg tgcatcgccc gtggtgctgc gggtgtttcg ccctttggca
      781 atctcagaca cagcgttaaa cgccatatcg accgattgct taggcaattt gcggatatcc
      841 gcatcaccga caacctggcg aaccagcgtt ttgtcagcag aagccagaac ctcgcgtttg
      901 aacacggtcg gtttcatctt acggctcaga tcgatacccg gaacgataac ttcggcacgc
      961 caggctgagt caccagtaat cgtggtttcc tcttcatcgt cctcgccgtc accggtcgga
     1021 ttatcgtcag gtttattatc gttatcgccc gtggcatttc cttccagctt agccagcagg
     1081 gctttcagta atgttttgag gtcatcatca ctgtcgccgg ttggacctcc gcccatctct
     1141 ggtactttgt ccggtagcgg ttgctgcggg gacaggttga tgttgagatt aacgccctgc
     1201 ggcaaatccc cctcatctcc tgtaaccgat gcgggagccg actccaccag ttcgttcatg
     1261 gtgtcagcgt cacccgtttt gatggccgtg cgcatgcggg tccaccagct tttcttttga
     1321 tttgccattg tgtctctgtc tccaattgca caacgatttc cggctctgcc tttggggaca
     1381 agagccacat ggtttccggt aatatcgacc tgctcggctt tacctggctc ggtctgctcg
     1441 tactccgcgt catagccgca cgacacttca cgcaggccat cttcgataag ctgaatggcg
     1501 ttttcgtctt tgacgataag gtcagccagc atcaaatcag agtggtcacc cgtcccgcgc
     1561 cgcacattct gaagatgccc gacagcaagc tctttccagt tctcgggatt taccagccgc
     1621 acattcccgt tttcatcttc aggatgcagg atcgtgatgc tcatcccttc gaatgaggcg
     1681 agcgtggccg gatggaatac ctgctcagga gagcgcgtta cgactatctc accgagcttg
     1741 tcgggtttga ggtttggcag atcggcagcg ccgtagagct gtttacccgt tcgacctatc
     1801 ggcacgtctt tgcacagcag cgagccgtca gccagctgat agcgggtttc ccccagccgg
     1861 gtattgaaaa aatatttcat ggtttacctg cgattcaggc gagataagaa tgagggttgg
     1921 gaaaaacgat ttctttataa cagcgacaat tcgggagctc gccagcgtga ccggtcatgc
     1981 catcaagcgt tggaggtttg ccccattcga caaacttacc ttccatctcc cgatgagaat
     2041 gccggacgtc gccatcttcg gctgtacgcc agatataacc attcgagccg attgacagcg
     2101 cacgcgcctg atcgagcgcg ccggttgcac gtccaagctc ggtacgggcg ataaggttcg
     2161 ctcgtgagcg tgacacgtca ccggacgctg ctatctcttt cgcgaatggt tcagcgcggc
     2221 caccagccac aacggcctcg atggccttgt tctgaatgtc atacacccga tcggcggcct
     2281 caagaggcag tgacttgata tacttaattt gctcggcgac gatggatttc atcacctggc
     2341 ctaccggggc gcggtcgacc atgttgcgca gctctgcgct gatgttccgg ctgtgctgac
     2401 gccactgctt ttcattctgg cgtgcaatgt cggcggtaaa gttctcagca accttcgtcg
     2461 cccagggggt gatgatttcg ctgtagcgct ccagcgcatc cattatttcg gtaacgctat
     2521 catttgaacc atcgtagcgc ccatttacga tatccccgac cgcccgcgct atctgccgta
     2581 ggctcgttcg atatcggatc tccgcctggc ggctctggcg gtttgtcgcc aagttcgccg
     2641 atgcctggcg gcgcttcgtc ttcggcattc tcgatatcct cgtcggtaat ggatgccccg
     2701 atgccggtga cgtcagagtt ttcgcgcagg tcggtcatcg ccgccttacg cgtcatcaat
     2761 ccgtcgccca gcgcggtact aatcgcgttg gtggtgttta cggccaccgt tgatcggtca
     2821 acgtctgaca tttgccatag cgggttaaac tcaaacgtga aatcgtccgg cagcggcttt
     2881 ccgagttccg agcggtgcat aatgtccagt atccggcgca tcggcagccg taagcggcgc
     2941 tcctgcaatg agctcacccg gtcgtaatag ttggcgaggt ctgcatcacc agtagagaag
     3001 cctttcgggg attgaccgaa caaccgcacc agcgggatac caacggcacc gctaatctgc
     3061 tcagcaaact gcgaaagaat gtcatccaga ccactaaagc tgtactggtg ggtttcgaac
     3121 ttatcccgcg agtccatgag cgtcatacct tcattgctct ggaactggcg gatcaggtcg
     3181 atgttcttca gcaacgcctc gaacgccggg cctccaagcg cgataagctc gcgcaacttc
     3241 tccacgctat aggtacgcag atgtgctttg tagaccagct gcgccgcgcc gacagtggcg
     3301 ctgtcgaacg cagtaagccg atcccagata cgctctacaa ccgacattcc ccattcgttt
     3361 tcggtcatct tctgctggaa tggcagcgtc accccgtcga agcgaatcag gcggctgtga
     3421 tggatgcgcc aggccgggat gcccgttgca gtggtcacca cgtcgtaaaa ctcaggtttg
     3481 ccgagatctg gccccatctc tttaatgcgg cgggtcagga ccgggttaat catccagcgg
     3541 tcgagcggga gaatgccctt aaacttgcct tctccaatgg tttcgagccg cagcggggtc
     3601 attggtgcct gcccctcgat catgatgaag ccgaccgcgc cgccgtagag gcgagaccat
     3661 ttcagcacgt cgttcagcgc atcccagatt tgcaactcat ccagttgtga ttcgagaatg
     3721 ccacgatctt ttgcatcaat ttccgaagtg atgcgaatgc ctttgcgggt catatcatcc
     3781 gggatagcat cgaccgcttc gccgataatc caggacgaac gataggacca ttccaccagc
     3841 atgcggttac gactggtgaa attagcccgg taggtggatg ctgagtgctg gttaggtgtc
     3901 tgcatcccca cgcgggcaac aaagttctca tagccatcag cggtggcctg tgccgttcgc
     3961 cgcgtggctt gtttgtttcg tgccatcagg cctgtctccc tagcagctcc cagatgttca
     4021 gggctgaatt cattggcgcg tagctgatca tcaccgagtc ggcaaggttt ggcgaccggg
     4081 ttccatcagg ctgtttatca ataacgattt ttcccacacc attaatggaa taggtcggct
     4141 gcgaaagctc gatgatgagt ttatctttga gtgccatgct actgctgatt gagatgattt
     4201 cgtccgggtt gtaatccata ccttcaacca cggcgcgcca ggtattctga aaaagtttac
     4261 gtaaccgcca ccagctctgg gctttggcgt tagcgaagaa gtccttgttc aggcgggcgg
     4321 cctgtccgtt gtcaccgcgc accgcttcgt cgtccggatc aaataccgcg ccactacctc
     4381 gaaacggtgt ggcgagtatt gacggtcggc gcgcagcgtt acgcagttcg ttgatagcgc
     4441 gtgcatcgcc gcgaacgcca gcgcccaacc cgtcctcgtc aaagcgaaac tcttcgaggt
     4501 tgtcctgttc gcaaaagccg aaaaccttct cgacggactg ataaatgtcg ctgcccacac
     4561 cggaccattc ccgcacattc tccaggagga agccatgacg ggtggaaaag gaatttttgt
     4621 ccctgccttc gtcggcgacg tccatcgcgc ccagtcgctt gcctgttggc tggatgccca
     4681 gcttgatatg cgcgtcgaca gcagcctgta cccattcgga tggaatcagg acgccttccg
     4741 ctgatgcact gtagttcagg tcaagttcct gtgccaccac caccggattg tcgattttct
     4801 cgcattccct gcgataccac tcttcatcct tgcgaggatc atcccgccag tggaatgtga
     4861 ataccggtat cttcccgcca tgacgcttct gagcgaacgg gttcgccatg ccgttaactg
     4921 aactcaggtc aatacggcaa cgcgtcgttt gtgacaacgc cgcatcaatc agcagaggac
     4981 gctgaaggaa tgcagactca tcaaccagat aaagcgtggt acggtcacca cggccaatat
     5041 tatcgccagc ctcacctttg ataacggcac cagtttcagg aaactcaacg cgcatgtatg
     5101 gtgcgtgctt cttttcgtcc cacgaacctc gaaactctac aggtagtgtt tccacgaact
     5161 tgcgcgcctt ccagaacaat gctttcgggt caccggtgct gtcgacgtat tcctctttac
     5221 gggagccgaa accgataacc atttctttgt tgaagagaca aagcgagcat gccagcccga
     5281 tcgcggtcca actgagcccc atttcacggg atttttcggt aataccattc tcccgattac
     5341 cccagcgttc cataatccag tggatccact cctcctgctt agggaagagt aaaaacggaa
     5401 tggtcaccgg caggccataa tcaatattac gcgggtccgt tgtcatgccc cagtcgatga
     5461 tgaactgagc cggattggtt cggtaaaact gttttagtgc aggcaatatt tcaggattct
     5521 ggcgaatgcg ctgtaggcgt tccatccgcc attcaaaaac catctggtaa tcaggatgtt
     5581 taaaatcgaa ggggaatggt aacggcatac ttagcccatc atttttctat acgcctctgc
     5641 agcctgctcc ggcgttaagt tggtaatttc tgttctgact ggtcctccgt cagcaccagt
     5701 cacttcattt ttgacattgt ctttaaacgc ctgaacagaa acatgacgcc cgagcaactc
     5761 aaggttttta accttatcag gccatttgat tttcttcagg agagcagcac tatctgctga
     5821 tgccatctcg acgacatcca ttcctgatag cgttgtacgc cacaccttag gccagtcttt
     5881 aatctgcttc agttcgccat tggcaagcag aatgtcaagc acgtccatct gatcgatttc
     5941 agtcagccgt cgcaatacat aggcggcgtc tatacctacc tcttcattgc gttcagcctt
     6001 tagctcagca atgcgatctg ctatgtcagg ttttgacagg ttctcgctac cagtggcgcg
     6061 agcagttttc tcgctgtaac ccgccctgat agctgcctga gtggcgttta aatcttttaa
     6121 atattccctg cagaacagtt cctgcttagg tgtgagctgt ttcaaatcac acctccgaaa
     6181 cattgctcca atttctatga ttaacgatgt cagcaatcat ggtagtggat acgccatatc
     6241 tctttgctaa agacctaagc ccattaccta cactcccgcg cacatactcg cggcgaatgc
     6301 agatgacatc gcactctttt aatttcgcca ttccgttctt ttcacctgaa cggtcagggt
     6361 atgagtttct gcctcgcctt gacctgtcac gattattgtc agcgacagta cctataatca
     6421 gatggtcagg attcacgcat gatggattat cgcatttatg cctaacaaca aggtccttg
//
LOCUS       DAJTUN010000165         6320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTUN010000165 DAJTUN010000000
VERSION     DAJTUN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.166"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..881)
                     /locus_tag="OO470_003582"
     CDS             complement(<1..881)
                     /locus_tag="OO470_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6252697.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPIT"
     gene            complement(960..1913)
                     /gene="lsrR"
                     /locus_tag="OO470_003583"
     CDS             complement(960..1913)
                     /gene="lsrR"
                     /locus_tag="OO470_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6252698.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2162..3697
                     /gene="lsrA"
                     /locus_tag="OO470_003584"
     CDS             2162..3697
                     /gene="lsrA"
                     /locus_tag="OO470_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6252699.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3691..4719
                     /gene="lsrC"
                     /locus_tag="OO470_003585"
     CDS             3691..4719
                     /gene="lsrC"
                     /locus_tag="OO470_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6252700.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4719..5711
                     /gene="lsrD"
                     /locus_tag="OO470_003586"
     CDS             4719..5711
                     /gene="lsrD"
                     /locus_tag="OO470_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6252701.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5723..6320
                     /gene="lsrB"
                     /locus_tag="OO470_003587"
                     /pseudo
     CDS             5723..6320
                     /gene="lsrB"
                     /locus_tag="OO470_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1409 a         1624 c         1666 g         1621 t
ORIGIN      
        1 gtaatcggca actgatgccg ggcctcgctg agcgtgcact gcttattatc cgtgaacgtg
       61 ccgccaagaa ccgcggtttg tgccggacgc acaacgccta acccaaggca accaagctgc
      121 acgtcgcctc ctccaacgac caccggagtg cccgccttca gaccgcagag ttccgccgct
      181 tgtgaactta ccacgcccag caatgtgccg gtttctttga caggagaaag aatatcggca
      241 cgcaggccag ccatatccag caatgcaggt ttccaggcac gggtggttag atcaagaagt
      301 cccgtggtgc cagcgttaga gggatccacc gccagttcgc cgctgagcat ataggccagc
      361 cagtcgctga tcatggtgat ggttgatgcc tgacggtaaa tatcggaacg atggtgcgcc
      421 agccaaagta atctggggat ggcacttaaa gccagtgttt gtccggtcgc tcgataaact
      481 tcgttttcaa aggtattgtt gtgcagttct ttaagttcgc taacttcgcg tgccgctctg
      541 gcatccacat tggcgcaggc ccagatcggt gttccttcat tgttatataa aacaatgcct
      601 tcacgcatag aacatgccga aacggcagcg atatactccg ggtctatgcc ggcgttgtgc
      661 agcgcctggc gcatacactc acacgccagt tgccagtttt tgttgagatc aaattccata
      721 gaaccaggaa cgtccggtac tgccagatgc cgccactccg cctgtcccac tgctatttga
      781 ttgccttcca ggtcgaatat cacagcccga atacttccgg tgcctgcatc cagcgccatc
      841 aggtagtact ttgattctga aggggtaaag agtcgagcca ttagattatc ctcggcttat
      901 gcgttctgtc tgttcctcta tacgttctcc atcattcccg gtaataaggt catgcaaatt
      961 taactacgta aaatcgccgc tgctgtgtcc tgatcggtaa ccagtgcgtt gatataaccg
     1021 cctttcattg cagcggcaat tgcttcggct ttattttctc cccctgccac gccaacccgg
     1081 acgggtatgg tcttcagcgc gcttaaaggt aagccaatca gttcgttatg tattttgata
     1141 tccgtgacaa cgtcaccttt tgcatcaaaa aagtagccta aaatgtcgcc aaccgcccct
     1201 tttcggccaa tcattaactg ttcgccctgg ctgatataac cggagcgaat gattgtcgcg
     1261 tcgtcttgtt gactcacggc tccaatgccg acaatcgcca catccgctgc ttgcgcggct
     1321 agcagaacat ctttgacgca attttcattt tttagcgtac gggcaatgtc agcggaggat
     1381 gcccgcaacg gagccggaat aatattcaca ctgcacgccg cgttaagctg cccgatgccc
     1441 gtcatataag aaccgacgcc accggagagc gtgaccaggc gaatttgctg tgacgaaata
     1501 aaaccactta agcgttgcag cgtattcatg gttgcctcgc caaaaccaat cgccagcatc
     1561 tgttgtggtt gaagtaaact catcaacata tgcgccgcgc ctatccctag tcgcccgccg
     1621 acatcagcat ccgcaagccc agggatcacc cggacatgtt gcagcgaaaa ctgacgacgt
     1681 aattgagttt catattccag acagccttca aagcgagaat taatctgtac gcgaataatg
     1741 ccggactgat gccctttctc cagcaagcgc gacactttca aacgggtcag gccgagacga
     1801 tcgctgatct cgctctgggt cagcccgtcg tgatagtaaa accacgcgat ccgcgcgacc
     1861 tgttcttctt cacacattcc ctgttctgaa attgccgaat cgttgattgt cataattcat
     1921 tcttcacttt gaacatattt aaatctttaa tgcaattgtt cagttcttgc gcatttatat
     1981 ctgtgatggc aaccacagtt tgactctacg agcatgaaca aacgcaaccg tgaaaatcaa
     2041 aatagcataa attgtgatct attcgtcgga aatatgtgca atgtccacct aaggttatga
     2101 acaaattaaa agtagaaata catttgttca aaactcacct gcaaaactga acgggggaaa
     2161 tatgcaaacg agtgataccc gcgcgttacc gctactttgc gcccgctcgg tttataaaca
     2221 gtattcaggg gtcaatgtcc tgaaaggcat cgattttacg ttgcatcagg gggaggtcca
     2281 cgccctgctc ggcggcaatg gtgccggtaa atcgacgtta atgaagatta ttgccggtat
     2341 tacccctgct gatagcggta cgctggagat tgggggcaac aactacgcca gattaacgcc
     2401 agttcatgct catcagctgg gcatttatct cgttccccag gaaccgctgc ttttcccaag
     2461 cctgtcgata aaagaaaaca tcctgtttgg gctggcaaaa aaacagctct ccatgcagaa
     2521 aatgaagaac ttgctggcgg cgctgggctg ccagtttgat ctgcatagtc tggcaggatc
     2581 gctggatgtc gccgatcgcc aaatggtgga aatcctccgc gggctgatgc gcgactcgcg
     2641 gattctgatc ctcgatgaac ctaccgcctc gcttacccct gcggaaaccg aacgcttgtt
     2701 tagtcgcttg caagagctgc ttgctactgg cgtgggtatt gtttttatct cgcataagct
     2761 gccggaaatt cgccagattg ccgatcgaat tagcgtgatg cgcgacggaa ccatcgactt
     2821 aagcggcaaa accagcgaac tgtctaccga cgacattatt caggccatca ccccagcggt
     2881 acgggaaaaa tcgctctctg ccagccaaaa attatggctg gagttacctg gtaaccgccc
     2941 acaacatgcc gtcggaacgc cggtgctgac actggaaaat ctgactggcg aaggcttcag
     3001 gaatgtcagc ctgacgctca atgccggaga aattctgggc ctggctgggc tggtgggagc
     3061 cggacgcaca gaactggccg agacgctcta tggtctgcgt actttgcgtg gcggacgcat
     3121 tatgctgaat ggtaaagaga tcaataaatt atccactgga gaacgtttac tgcgcggtct
     3181 ggtttatctg ccggaagatc gccagtcatc cggactgaat ctcgatgctt cgctggcctg
     3241 gaacgtctgc gcccttactc ataaccttcg tggattctgg gcgaaaaccg cgaaagataa
     3301 tgccactctg gaacgttatc gtcgggcgct gaatattaaa tttaaccaac cggaacaagc
     3361 tgcacggaca ttatccggta gcaaccagca aaaaatcctc attgccaaat gcctggaagc
     3421 ttcgccgcaa gcattgattg tcgatgagcc gacgcgcggc gtggatgtct cggcccgtaa
     3481 tgatatctac cagctgttgc gcagcatcgc cgcacagaat gtggctgtgc tgtttatctc
     3541 ctctgatatg gaagagatcg aactgatggc agatcgtgtg tatgtgatgc atcagggcga
     3601 aattacccac tctgcactga ccgggcgcga tattaatgtc gagactatta tgcgcgttgc
     3661 cttcggcgat agtcagcgtc aggaggcatc atgctgaagt ttattcagaa caaccgtgaa
     3721 atcacggcat tgctggcggt ggtgctgctg tttgtattac ccggttttct cgaccgccag
     3781 tatttaagtg tgcaaacgct gaccatggtt tatagcagcg cgcaaatcct gatcctgctg
     3841 gcaatgggcg cgacgctggt aatgcttacg cgcaatattg atgtttcagt gggttcgatt
     3901 accggaatgt gcgcggtgct gttggggatg ttactgaacg caggatattc actacctgtt
     3961 gcctgtgtcg cgactttact gcttggtttg ctcgcgggat ttttcaacgg tgccctggtc
     4021 gcgtggctaa agatccctgc cattgttgcc acccttggca cgttagggtt gtacagaggc
     4081 atcatgttgc tgtggactgg cggtaaatgg attgaagggt tacccgccga actgaaacag
     4141 ctctccgccc cgctgctgct tggcatttca gcaattggtt ggttgacgat aattctggtg
     4201 gcatttatgg cctggctgct ggcgaagacg gcgtttggac gcagttttta tgccacgggc
     4261 gataatttac agggcgctcg tcaactgggc gttcgtacgg aagccattcg cattgtggca
     4321 ttttcgttga acggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt
     4381 tttatcctca accagaccgg taccgggctg gagatgaaag caattgcagc ctgcgtgctg
     4441 ggcggcatta gtttgctcgg tggttccggt gcgttcatcg gtgcggtact cggcgcatgg
     4501 ttcctgacgc agatcgatag cgtactggtg ctgttgcgca ttccggcatg gtggaatgat
     4561 tttatcgcgg gtctggttct gctggcggtg ctggtgtttg atggacgcct gcgttgtgct
     4621 ctggaacgta atctacggcg gcaaaaatat gcccgcttta tgacgccacc gccatccgtt
     4681 aaacccgctt cgtcaggtaa aaaacgggag gccgcataat gcgtattcgc tacggttggg
     4741 aactggctct tgccgcactg ctcgttattg agattgtcgc atttggtgca attaacccgc
     4801 gaatgttaga tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg
     4861 tcgctctacc gctgacgatg gtgattgtca gtggcgggat cgatatttcg tttggttcga
     4921 ccatcggcct ctgcgccatt gcattgggcg tactgtttca aagtggtgtg ccgatgccgc
     4981 tggcgatact cctgacctta ctgctcggcg cattgtgcgg gctgatcaac gccggattaa
     5041 ttatctatac caaagttaac ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg
     5101 gaagcgctct gctgctttcc ggtatggccg gagcgacggg gtacgaaggt attggtggat
     5161 tcccgatggc gtttacagat ttcgctaacc tggatgtgct gggactcccc gttccgctga
     5221 ttatcttcct gatatgtctc ctcgttttct ggctctggct gcataaaacc catgccggac
     5281 gtaatgtgtt tttgattggg caaagcccgc gcgtggcgct ttatagcgcg attccagtta
     5341 accgcacctt atgtgcgctc tatgccatga cggggctggc gtctgcggtc gccgctgtgc
     5401 tgctggtatc gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg
     5461 ccatcaccgc cgtggtgctt ggcggggcca atatttatgg tggttccggt tccattatcg
     5521 gcaccgccat tgcggtttta ttagtgggat atttgcagca aggtttgcaa atggcaggag
     5581 tgccaaatca ggtgtccagc gccctttccg gtgcgctgct tatcgtcgtt gtcgtaggtc
     5641 gttccgttag cctgcatcgc cagcaaatta aagagtggct ggcgcgtcgg gccaataacc
     5701 cattgccata aaggatatct tcatgacact tcatcgcttt aagaaaatcg ccttacttag
     5761 cgctcttggc attgccgcaa tctctatgaa tgtgcaggcc gcagagcgta ttgcatttat
     5821 tcccaaactg gttggcgtgg gattttttac cagcggtggc aacggcgcac aacaagcggg
     5881 taaagagctg agcgttgatg tgacctacga cgggccgaca gaacccagtg tttctggtca
     5941 ggtacagttg attaataact tcgtcaatca aggttataac gccattattc gtttctgcgg
     6001 tttcgcctga tggcttgtgt ccggcactga aacgcgccat gcaacgtggt gtgagagtgc
     6061 tgacctggga ctctgatact aaaccggagt gccgctctta ctacattaat cagggaacgc
     6121 ccgcccagtt gggaggtatg ttggtggata tggcggcgcg tcaggtgaat aaagacaaag
     6181 ccaaagtcgc gtttttctac tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag
     6241 aagcgaaagc gaaaatcgcc aaagagcatc caggctggga aattgtcact acgcagtttg
     6301 gctataacga tgccactaaa
//
LOCUS       DAJTUN010000166         6080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTUN010000166 DAJTUN010000000
VERSION     DAJTUN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.167"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO470_003588"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO470_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6252702.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO470_003589"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO470_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6252703.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO470_003590"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO470_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..>6080)
                     /locus_tag="OO470_003591"
     CDS             complement(5797..>6080)
                     /locus_tag="OO470_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6252704.1"
                     /translation="ILNNFARFILLVPQEGVTHNAQGKATALILDKDDVVQLREIEAS
                     KAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
BASE COUNT         1595 a         1432 c         1440 g         1613 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcaa
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat
//
LOCUS       DAJTUN010000167         6069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTUN010000167 DAJTUN010000000
VERSION     DAJTUN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.168"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            292..819
                     /locus_tag="OO470_003592"
     CDS             292..819
                     /locus_tag="OO470_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6252705.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            877..1110
                     /locus_tag="OO470_003593"
     CDS             877..1110
                     /locus_tag="OO470_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCR6252706.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNQYIRDFGILK"
     gene            1169..3127
                     /locus_tag="OO470_003594"
     CDS             1169..3127
                     /locus_tag="OO470_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6252707.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYAVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDARAYRNLPEPEAVLTEAED
                     ERLNELMARYDSLENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CIQRGVQLRSEDDVADDADRTEQGLESASVEEISLPLLTKLTSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSKAHSSPARIRLECEHYALTSDAPSGKEGAAFMALMAEHSRF
                     AALLPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATEHTANLADAA"
     gene            3182..3616
                     /gene="psiB"
                     /locus_tag="OO470_003595"
     CDS             3182..3616
                     /gene="psiB"
                     /locus_tag="OO470_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCR6252708.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWVMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3613..4332
                     /locus_tag="OO470_003596"
     CDS             3613..4332
                     /locus_tag="OO470_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCR6252709.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            4329..4925
                     /locus_tag="OO470_003597"
     CDS             4329..4925
                     /locus_tag="OO470_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252710.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            5387..5887
                     /locus_tag="OO470_003598"
     CDS             5387..5887
                     /locus_tag="OO470_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR6252711.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         1272 a         1628 c         1907 g         1262 t
ORIGIN      
        1 cccgctgtgg actttccggg gggcagacgt aaccccctcc cgttccatct gtccagggtg
       61 agatgcaccg gccacaaaat ttttagccat gaacccggca aaaaattttg cgtccgcccc
      121 cggacagctg ccccgtgagg cggtaaacgg gcccccatcg gaaaggtcca ctgcggggag
      181 aaacccctgt gaagtggacg gcaggaggag ctttcgctcc tctgaagggg cacatgaacg
      241 tcccgtcgcc ggtatccggt ttatctcaca tctttcagtc aggaggttct tatgagtgca
      301 cgtggtatca acaaggtcat cctcgtcggg cgtctgggca atgatccgga agtccgttac
      361 atccccaacg ggggcgcagt ggcaaacctg caggtggcca catcagaaag ctggcgtgac
      421 aaacagacgg gggagatgcg ggagcagacg gaatggcacc gcgtggtgct gttcggcaag
      481 ctcgcggaag tggcaggtga atatctgcgc aagggtgcgc aggtctacat cgaaggtcag
      541 cttcgcaccc gtagctggga agataacggt atcacccgtt acgtcactga aattcttgtt
      601 aagaccacgg gcaccgtgca gatgctggga cgtgcaccac agcagaacgc tcaggcgcaa
      661 ccgaagcctc agcagaatgg gcagccacag agtgctgacg cgacgaaaaa aggtggcgcg
      721 aaaacgaaag gccgtggacg taaggccgcg cagccagagc ctcagccgca accgccggag
      781 ggggaggatt acgggttttc agacgacatc ccgttctgac tggcctgact gtgacaaccg
      841 ccccgccctg tgcggggcat caccggagat atgaggatga gcgaatattt cagaatactt
      901 cagggactgc cggacggctc ctttacccgc gaacaggcgg aagccgttgc cgcacagtac
      961 cggaacgtct ttatcgagga tgatcaggga acgcattttc gcctggttgt ccgtcaggat
     1021 ggcacgttga tctggcgctc ctggaatttt gaggactgtg ccgggtactg gatgaaccag
     1081 tacatcaggg atttcgggat tcttaagtaa gagaggtgcc ggacgcgcga acgtccggca
     1141 ggacataagc aattataagg ggatgattat gcctgtaacg aagtgtgaac cagaaaccac
     1201 ccgcaaagca agccgtaaat ctgtaaaaac gcaggaaact gtcctgtctg ccctgctggc
     1261 gcagacggag gaagtgagcg tgccgctggc ctcgctgatt aaatcaccgc tgaatgtgcg
     1321 cacggtgccg tattctgcgg agtccgtcag cgaactggcg gagtccatta agggcgtcgg
     1381 actgctgcag aatctggtcg tgcataccct gcctggtgac cgttacgctg tcgccgcagg
     1441 cggtcgccga ctggcagcac tcaacatgct ggcagagcgc aacatccttc cggctgactg
     1501 gcctgtacgc gtgaaggtca ttccgcagga gctggcgact gccgcatcga tgaccgagaa
     1561 cggtcagcgt cgggatatgc accctgccga acagattgcc ggattccgcg caatggcgca
     1621 ggaaggcaaa acgcctgcac aaatcggtga tttgctgggc tattcacccc gccacgttca
     1681 gcgaatgctg aaactggcag accttgctcc ggttatcctc gatgcgctgg cagaagaccg
     1741 catcaccaca gagcactgtc aggcgctggc gctggagaac gacaccgcgc gtcaggtgca
     1801 ggtgtttgaa gccgcctgcc agtcgggatg gggcggtaaa ccggaagtac agaccattcg
     1861 tcgtctggtg accgaaagtg aagtggcggt ggcggggaac agtaaattcc gcttcgtggg
     1921 ggctgatgcc ttctcgccag acgaactgcg caccgatttg ttcagcgatg acggggacgg
     1981 ttatgtcgac cgcgtggcgc tcgatgccgc cctgctggaa aaactccagg ctgtcgcaga
     2041 gttccttcgg gaagccgaag gctgggaatg gtgcgccgga cgcatggagc ctgtcggtga
     2101 gtgccgtgaa gatgcccggg cataccgcaa cctgccggag ccggaagcgg tgctgacgga
     2161 ggcggaagac gaacgtctga acgaactgat ggctcgttac gactcgctgg aaaaccagtg
     2221 tgaggaatct gacctgctgg cagcagaaat gaagctgatt gactgcatgg cgaaggtcag
     2281 ggcgtggacg ccggagatgc gtgccggaag tggcgtggtg gtgtcctggc gttacggtaa
     2341 tgtgtgtatc cagcgtggtg tgcagttgcg cagtgaggat gatgtggctg acgacgctga
     2401 ccgcacggaa caggggctgg agagcgcgtc agtggaggaa attagtctgc cgttgctgac
     2461 taaattgacc tcagagcgca cgctggcagt ccaggcagca ctgatgcagc aaccggacaa
     2521 atccctggca ctgctggcat ggacgctctg cctgaatgtg tttgacagca aagcgcatag
     2581 tagtcccgcc agaattcgcc tggaatgtga gcattatgcg ctgaccagcg atgcaccgtc
     2641 agggaaggaa ggtgccgcat tcatggcgct gatggcagaa cactctcgtt ttgctgccct
     2701 gctgccggaa ggatgggaac gggatatgac gacgttcctg tcacttagcc aggaggtgct
     2761 gttatccctg ctcagtttct gtaccgcgtg cagtcttaac ggtgtccaga cccgcgagtg
     2821 tggtcacacg tcacgcagtc cgctggactc gctggaaacc gccatcggct ttcacatgcg
     2881 cgactggtgg cagccgacaa aagcaaactt cttcggacac ctgaaaaagc cgcagattat
     2941 cgcagccctg aatgaggccg gactgtccgg tgccgcacgg gacgcggaga aaatgaagaa
     3001 aggcgatgcg gctgaacatg cagagttcca tatgaaagac aaccgctggg tgcctggctg
     3061 gatgtgtgca ccacgtccac agacggatgc cactgaacac accgctaacc tggctgatgc
     3121 cgcctgatga acaaccacac cgccccgccg gagacggggc ggcagcaagg gagataccgt
     3181 gatgaaaact gaactgaccc tgaatgccct gcagtccatg aacgcacagg aatatgaaga
     3241 tatccgtgct gcgggaagcg atatgcgccg taatctcact cacgaggtga tgcgtgaagt
     3301 ggacgcaccg gctaactggg tgatgaacgg agagtatggc agtgagttcg ggggcttttt
     3361 ccccgtccag gttcgtttca cgccagccca cgaacgtttc cacctggcat tatgttcgcc
     3421 gggagacgtc tctcagctct ggatgctggt tctggtgaat ggtggtggac agcctttcgc
     3481 cgtcgttcag gtgcaacata tcttcacgcc tgtcgcgatc agtcacacgc tggcgcttgc
     3541 cgcgacactg gatgcgcagg gatacagtgt taacgacatc atccatatcc tgatggcaga
     3601 aggaggtcag gcatgagcgc acgttcacgg gcactgatcc ccctcagcgc agaacaacag
     3661 gccgccatgc aggcggtggc tgtcacagaa caacgtcgtc gtcagggacg cacactttca
     3721 gcatggcctt atgccaccgc tttctttcgc tgcctgaatg gcagtcgccg gatttcgctg
     3781 accgatctcc gcttttttgc ccctgcgctg acgaaggagg aatttcatgg caaccgtctc
     3841 ctgtggctgg ctgccgtgga taaactgatt gaaagttttg gggaagtctg tgttcttccc
     3901 ctgccatccg atgcggggca ccgtctgttc ccgtccgtgc cttttcgtga aggtgagcgt
     3961 cgtcgtcaga aaaccacgct gacagagcag aaatacagcc gccagcggga acgtgaggca
     4021 gaacgacggg aactggaata ccagacatgt tttgctcagg cgcagattga ccttgcgttt
     4081 catactcccg ccacggtcgg aagctggttg tcccgctggt ctggtgttgt tgaggagcat
     4141 gatctggaaa cgattttctg ggggtggtgc gggcgttttc catcactgtc atcatttgac
     4201 cggtttttct ggcaggagga accactctgg cggctgattt ttgaagccgg tgaggccggt
     4261 cgtggtgcac cggtacaggt acgtgcactt gagcagtgga tgatcccgaa caagctggag
     4321 aacgtaatat gatgaaatca gacgaaaaat accaggttcc cgcgtggatg cgacctctgt
     4381 tgccgttgct ctgcaacacc ggggggaacg atccggaaga actgctgaat gatacagaaa
     4441 ccactgccag tgcgaatgtt gtccgttatg tactgatagt tgctgtgcgg tcgcaggttg
     4501 atctgctgca gcttctgtac aggaaagggc tgttgcgcac agagatacca ggtggctttt
     4561 caccggaaga agcgcaggca ctcctggata atctggtgcg cagccatatc agcaaggcgc
     4621 tgtcgggcga gcgaatggca gcccgtgaca gaaatgccga tctggcctgg attggacagc
     4681 aactggtcga tgccgcctgg tttgtccgtg ccacactgga agcgcatggg atgagcgtcg
     4741 gaaatgagag tccccctgct ccgccggaga caatgccgga catacagaca cgggaactgg
     4801 tgatgttgat caagcgactg gcatcatcgc tgaaagcggt gaaaccggac agttgtgtgg
     4861 tgcgtgaagc gcaggactgg ctgcgcgaca gaaaacttgt ggatatcaca gatattctcc
     4921 ggtgaaccag gcatgtatat tttacgcagg gacatgccgg tcatgatcgt ttcgttccag
     4981 gggtggtctg gagatcatgc ggtgtgctcc ctgctctcgc cggaataagg acgtcgccgc
     5041 cggctccgct tttacccggc catgcggggc atggccttgt gggttttcag ttatgtggcc
     5101 gccgtgtcgt gtgcgggctg tgccgtgcct ccatcgtagc cgggcttctg cgggcgggat
     5161 gcaaggtaag atgaaccgct gcggtcaccc ggtccctcct tcccgtctgc cgtgattttc
     5221 cggtcgttca cccgctcaga tttcgcggtc tcaccctcca actcccgcca gccgtgtgcg
     5281 tcgggctgcg gcttcccttg cgcccttgca tccctgcagg cccggcaaag atggaggcac
     5341 ggcaccgccc ggtgtcgcag aacatgtgac tatggaggat tcgggaatgt ctgttgttgc
     5401 acctgctgta tacgttggaa cctggcacaa atacaactgt ggaagcatcg ctggccgctg
     5461 gtttgacctg acaacgtttg atgatgaacg cgactttttc gccgcctgcc gtgcccttca
     5521 ccaggatgaa accgacccgg aactgatgtt tcaggattat gagggattcc cggggaatat
     5581 ggcctctgaa tgccatatca actgggcctg ggttgaaggc ttccgccagg cgcgggatga
     5641 aggctgcgaa gaggcttatc gtctctgggt ggatgatacc ggtgagacgg attttgacag
     5701 cttccgcgat gcctggtggg gcgaggctga cagtgaggag gcttttgcgg ttgagttcgt
     5761 cagtgatacc ggcctgctgg ctgacgtgcc ggagacggtg gcgctctatt ttgactatga
     5821 ggcgtatgcg cgggatttat tcctggactc cttcaccttt attgacggtc atgtgttccg
     5881 tcggtgattt actccccgct ccggcggggg gatggcctgc caggcccccg gaaaaccctg
     5941 gcaggcttac gcctgcctga aatacaggag ggccgcgcct gcggcgctac ccttcctgcc
     6001 tgtcatagtg tgaggcattt ttctgttttg tcgtttctgt tgttctgact cccgggggcg
     6061 gggacaccc
//
LOCUS       DAJTUN010000168         6068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTUN010000168 DAJTUN010000000
VERSION     DAJTUN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.169"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..335
                     /locus_tag="OO470_003599"
     CDS             <1..335
                     /locus_tag="OO470_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR6252712.1"
                     /translation="YPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSP
                     LVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEY
                     RDYRRTSI"
     gene            469..1479
                     /gene="adhP"
                     /locus_tag="OO470_003600"
     CDS             469..1479
                     /gene="adhP"
                     /locus_tag="OO470_003600"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6252713.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            1625..1909
                     /locus_tag="OO470_003601"
     CDS             1625..1909
                     /locus_tag="OO470_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6252714.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(2137..2790)
                     /gene="fdnI"
                     /locus_tag="OO470_003602"
     CDS             complement(2137..2790)
                     /gene="fdnI"
                     /locus_tag="OO470_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6252715.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(2783..3667)
                     /gene="fdnH"
                     /locus_tag="OO470_003603"
     CDS             complement(2783..3667)
                     /gene="fdnH"
                     /locus_tag="OO470_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6252716.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(3680..>6068)
                     /gene="fdnG"
                     /locus_tag="OO470_003604"
     CDS             complement(3680..>6068)
                     /gene="fdnG"
                     /locus_tag="OO470_003604"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6252717.1"
                     /translation="KNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTR
                     TASVADIYAPIRSGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGL
                     FSGYDAEKRQYDKSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENIC
                     GTPKADFLKVCEVLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMA
                     GGGVNALRGHSNIQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVN
                     YWSNYPKFFVSLMKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFC
                     QGFNPVASFPDKNKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVF
                     RLPSTCFAEEDGSIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGK
                     GVEPLMKMSWNYKQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRD
                     DGTTASSCWIYTGSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADI
                     NGKPWDPKRMLIQWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEG
                     PFPEHYEPIETPLGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFH
                     TWTKHALLNAIAQPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPL
                     NVNGQQVETVGIPIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         1421 a         1651 c         1546 g         1450 t
ORIGIN      
        1 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta
       61 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
      121 ctcagtattc gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata
      181 ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
      241 tggcggtcaa aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag
      301 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac
      361 cgggctattt ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
      421 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca
      481 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
      541 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
      601 aatggcgatt ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg
      661 gcagaagttg gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
      721 ttttacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
      781 tcagttaaaa atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc
      841 gccgattacg cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc
      901 tgtgcggggg tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg
      961 attgctatct acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc
     1021 tttaacgcca aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa
     1081 atggatgcag atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag
     1141 aaaaccggtg gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg
     1201 gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct
     1261 atgagcctgg atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc
     1321 ggcacgcgcc aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg
     1381 aaagtcgccc tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc
     1441 aaaatccgtg gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc
     1501 atgttcgggt cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg
     1561 cgtaggaagg aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac
     1621 actaatgaaa atggccaatc atccccgccc gggggacatt attcaggaat cactggacga
     1681 acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag
     1741 ccgattgctg accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt
     1801 gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc
     1861 agaaaaaacg gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc
     1921 ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat
     1981 caggcctaca cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca
     2041 ttcacgacgc atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt
     2101 ttacagcgcc attttcagct taaagttcaa aagagattat atcccttctt cactctcttt
     2161 tttcgcttct gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg
     2221 acggctcacc ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata
     2281 catatggatc aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct
     2341 gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc
     2401 ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc
     2461 gttgtactta ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt
     2521 caacagccac ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat
     2581 aaacatcagt gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg
     2641 cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga
     2701 cagcgccacc aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt
     2761 ggtgcgcaca atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct
     2821 tattcgggcc aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag
     2881 ccgctgccag cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat
     2941 ctttcggcag accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt
     3001 gcgtaccacc gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca
     3061 gtttcgccac gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag
     3121 ccccggtcgg acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc
     3181 agagcgtgca tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac
     3241 acccggcaat gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt
     3301 tagcgtactg aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac
     3361 agtgcataca gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc
     3421 taaagcgcat caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga
     3481 cgcagtgccc cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt
     3541 tacagccgat acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca
     3601 cctgagaagg cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca
     3661 tagccatctg ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg
     3721 gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac
     3781 gcgcgacacc ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt
     3841 ttacattcag cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct
     3901 tgctggagac agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc
     3961 tgatttccac aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg
     4021 tgtggaagtg ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac
     4081 ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat
     4141 gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt
     4201 ccgccatttt gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc
     4261 cggttggcgt acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc
     4321 tgccgttcca ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg
     4381 aagcacggtt gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat
     4441 tccccagacc ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc
     4501 agctaccggt gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa
     4561 agctactcag caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt
     4621 ccaacgcata gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct
     4681 tgtagttcca gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca
     4741 actcgcgcag atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg
     4801 gcgcgtcctg acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat
     4861 cctcttcagc aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat
     4921 cgacatcgtt cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat
     4981 cgataaccac catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga
     5041 aggacgcaac cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca
     5101 tgttgaaata cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc
     5161 agttgttctc tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact
     5221 tcggatagtt gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt
     5281 ccagatacga ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc
     5341 tggtagagag cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt
     5401 tcacgccgcc accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac
     5461 ggatgttctg cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg
     5521 tgcgatccgg tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg
     5581 gcgtaccaca gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca
     5641 ggttccacac acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat
     5701 cgaactgata gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga
     5761 acaggccatc ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt
     5821 taacgtattc ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga
     5881 acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg
     5941 taaaacgggg atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc
     6001 agcggaaacc gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag
     6061 cgtttttg
//
LOCUS       DAJTUN010000169         6035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTUN010000169 DAJTUN010000000
VERSION     DAJTUN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.170"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(28..654)
                     /gene="upp"
                     /locus_tag="OO470_003605"
     CDS             complement(28..654)
                     /gene="upp"
                     /locus_tag="OO470_003605"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6252718.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            979..2016
                     /gene="purM"
                     /locus_tag="OO470_003606"
     CDS             979..2016
                     /gene="purM"
                     /locus_tag="OO470_003606"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6252719.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2016..2654
                     /gene="purN"
                     /locus_tag="OO470_003607"
     CDS             2016..2654
                     /gene="purN"
                     /locus_tag="OO470_003607"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6252720.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2826..4892
                     /gene="ppk1"
                     /locus_tag="OO470_003608"
     CDS             2826..4892
                     /gene="ppk1"
                     /locus_tag="OO470_003608"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6252721.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4897..>6035
                     /gene="ppx"
                     /locus_tag="OO470_003609"
     CDS             4897..>6035
                     /gene="ppx"
                     /locus_tag="OO470_003609"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6252722.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSG"
BASE COUNT         1468 a         1500 c         1551 g         1516 t
ORIGIN      
        1 aaagtcggct ttaattattt ttattcttta tttcgtacca aagattttgt caccggcatc
       61 gccgaggccc ggaataatgt atccgtgctc gttcagtccc tggtcaatcg atgcagtata
      121 cagttcgacg tccgggtgcg ctttttccag cgcagcgata ccttctggcg cagcgaccag
      181 caccagaact ttgatgctgc tgcagcccgc ttttttcagc aggtcgatgg tcgcgataac
      241 ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc atacgctcat cgatgttaga
      301 aaccagtttc tggaagtacg gtaccggctc cagcgtttct tcattacggt acataccgac
      361 aacgctgatg cgcgcgctcg gaacgttttc cagcacaccg tccatcatac caagacccgc
      421 acgcagaatt ggcacaacgg taattttctt acctttgatc tggtcgattt ctaccgggcc
      481 gttccagcct tcgatagtta ctttttccgt ttcgaggtcg gcggtcgctt cgtaagtcag
      541 caggctaccc acttcggaag cgagttcgcg aaagcgcttg gtgctgatat cttgctcacg
      601 catcagtccc agcttgtgtt tgacgagtgg gtgtttgact tccacgatct tcatactctt
      661 tctcctttga ggggcagcca caaaaaaaat cgacggatta tacctccttt cttcaaggcg
      721 gcaatattat tttcgttgac tttagtcaaa atgataacag tttgagataa agttatttta
      781 tattcaaatg gttatgaaag aagattattc catccgaaaa ctaacctttg ccctggcaca
      841 agtcttcttt cgccgcgcgc ccggggaaaa gacgtgcaaa aaggttgtgt aaaacagtct
      901 cgcaaacgtt tgctttccct gttagaattg cgccgaattt tatttttcta ccgcaagtaa
      961 cgcgtgggga cccaagcagt gaccgataaa acctctctta gctacaaaga tgccggtgtt
     1021 gatattgacg cgggtaatgc tctggttgga agaatcaaag gcgtagtgaa gaaaacgcgt
     1081 cgtccggaag tgatgggcgg tctgggcggc ttcggtgcgc tgtgtgcatt gccgcaaaaa
     1141 tatcgtgaac ccgtgctggt ttccggcact gacggcgtag gtaccaagct gcgtctggca
     1201 atggacttaa aacgtcacga caccattggt attgatctgg tcgccatgtg cgttaatgac
     1261 ctggtggtgc aaggtgcgga accgctgttt ttcctcgact attacgcaac cggaaaactg
     1321 gatgttgata ccgcttcagc ggtgatcagc ggcatcgcgg aaggttgtct gcaatcgggc
     1381 tgttctctgg tgggtggcga aacggcagaa atgccgggga tgtatcacgg tgaagattac
     1441 gatgtcgcgg gtttctgcgt gggcgtggta gaaaaatcag aaatcatcga cggctctaaa
     1501 gtcagcgacg gcgatgtgct gattgcactc ggttccagcg gtccgcactc gaacggctat
     1561 tcgctggtgc gcaaaattct tgaagtcagc ggttgtgatc cgcaaaccac cgaacttgat
     1621 ggtaagccat tagccgatca tctgctggca ccgacccgca tttacgtgaa gtcagtgctg
     1681 gagttgattg aaaaggtcga tgtgcatgcc attgcgcacc tgaccggcgg cggcttctgg
     1741 gaaaacattc cgcgcgtatt gccagataat acccaggcag tgattgatga atcttcctgg
     1801 cagtggccgg aagtgttcaa ctggctgcaa acggcaggta acgttgagcg ccatgaaatg
     1861 tatcgcacct tcaactgcgg cgtcgggatg attatcgccc tgcctgctcc ggaagtggac
     1921 aaagccctcg ccctgctaaa tgccaacggt gaaaacgcgt ggaaaatcgg tatcatcaaa
     1981 gcctctgatt ccgaacaacg cgtggttatc gaataatgaa tattgtggtg cttatttccg
     2041 gcaacggaag taatttacag gcaattattg acgcctgtaa aaccaacaaa attaaaggca
     2101 ccgtacgggc agttttcagc aataaggccg acgcgttcgg ccttgaacgc gcccgccagg
     2161 cgggtattgc aacgcatacg ctcatcgcca gcgcgtttga cagtcgtgaa gcctatgacc
     2221 gggagttgat tcatgaaatc gacatgtacg cacccgatgt ggtcgtgctg gctggtttta
     2281 tgcgcattct cagcccggcg tttgtctccc actatgccgg gcgtttgctg aacattcacc
     2341 cttctctgct gccgaaatat cccggattac acacccatcg tcaggcgctg gaaaatggcg
     2401 atgaagagca cggtacatcg gtgcatttcg tcaccgatga actggacggt ggcccggtta
     2461 ttttacaggc gaaagtcccg gtatttgctg gtgatacgga agatgacatc accgcccgcg
     2521 tgcaaaccca ggaacacgct atttatccac tggtgattag ctggtttgcc gatggtcgtc
     2581 tgaaaatgca cgaaaacgcc gcgtggctgg atggtcaacg tctgccgccg cagggctacg
     2641 ctgccgacga gtaatgcccc cgtagttaaa gcgccagctc tgccgctggc gtttttcaat
     2701 tcacctgtaa atcgcaagct ccagcagttt ttttccccct tttctggcat agttggacat
     2761 ctgccaatat tgctcgccat aatatccagg cagtgtcccg tgaataaaac ggagtaaaag
     2821 tggtaatggg tcaggaaaag ctatacatcg aaaaagagct cagttggtta tcgttcaatg
     2881 aacgcgtgct tcaggaagcg gcggacaaat ctaacccgct gattgaaagg atgcgtttcc
     2941 tggggatcta ttccaataac cttgatgagt tctataaagt ccgcttcgct gaactgaagc
     3001 gacgcatcat tattagcgaa gaacaaggct ccaacgctca ttcccgccat ttactgggca
     3061 aaattcagtc ccgggtgctg aaagccgatc aggaattcga cggcctctac aacgagctat
     3121 tgctggagat ggcgcgcaac cagatcttcc tgattaatga acgccagctc tccgtcaatc
     3181 aacaaaactg gctgcgtcat tattttaagc agtatctgcg tcagcacatt acgccgattt
     3241 taatcaatcc tgacactgac ttagtgcagt tcctgaaaga tgattacacc tatctggctg
     3301 tggaaattat ccgtagcgat agcatccgtt acgcgctgct ggagatccca tcagataaag
     3361 tgccgcgctt tgtgaattta ccgccagaag cgccgcgtcg acgcaagccg atgattcttc
     3421 tggataacat tctgcgttac tgccttgatg atattttcaa aggcttcttt gattatgacg
     3481 cgctgaatgc ctattcaatg aagatgaccc gcgatgccga atacgattta gtgcatgaga
     3541 tggaagccag cctgatggag ttgatgtctt ccagtctcaa gcagcgttta actgctgagc
     3601 cggtgcgttt tgtttatcag cgcgatatgc ccaatgcgct ggttgaagtg ttacgcgaaa
     3661 aactgactat ttcccgctac gactccatcg tccccggcgg tcgttatcat aattttaaag
     3721 actttattaa tttccccaat gtcggcaaag ccaatctggt gaacaaacca ctgccgcgtt
     3781 tacgccatat ttggtttgat aaagcccagt tccgcaatgg ttttgatgcc attcgcgaac
     3841 gcgatgtgtt gctctattat ccttatcaca cctttgagca tgtgctggaa ctgctgcgtc
     3901 aggcttcgtt cgacccgagc gtactggcga ttaaaattaa catttaccgc gtggcgaaag
     3961 attcacgcat catcgactcg atgatccacg ccgcacataa cggtaagaaa gtcactgtgg
     4021 tggttgagtt acaggcgcgt ttcgacgaag aagccaacat tcactgggcg aagcgcctga
     4081 ccgaagcagg cgtgcacgtt atcttctctg cgccggggct gaaaattcac gccaaactgt
     4141 tcctgatttc acgtaaagaa aacggtgaag tggtccgtta cgcacacatc gggaccggga
     4201 actttaacga aaaaaccgcg cgtctttata ctgactattc gttgctgacc gcagatgcgc
     4261 gtatcaccaa cgaagtacgg cgggtattta actttattga aaacccatac cgcccggtga
     4321 catttgatta tttaatggtg tcaccgcaaa actctcgccg tctgttatat gaaatggtag
     4381 accgcgaaat cgccaacgcg cagcaagggc tgcccagtgg tatcaccctg aagctaaata
     4441 accttgtcga taaaggcctg gttgatcgtc tgtatgcggc ctccagctcc ggcgtaccgg
     4501 ttaatctgct ggttcgcgga atgtgttcgc tgatccccaa tctggaaggc attagcgaca
     4561 acattcgtgc catcagtatt gttgaccgtt accttgaaca tgaccgggtt tatatttttg
     4621 aaaatggcgg cgataaaaag gtctaccttt cttccgccga ctggatgacg cgcaatattg
     4681 attatcgtat tgaagtggcg acaccgctgc tcgatccgcg cctgaagcag cgggtgctgg
     4741 acatcatcga catattgttc agcgatacgg tcaaagcacg ttatatcgat aaagaactca
     4801 gtaatcgcta cgttccccgc ggcaatcgcc gcaaagtacg ggcgcagttg gcgatttacg
     4861 actacatcaa atcactcgaa caacctgaat aaccctatgc caatacacga taaatcccct
     4921 cgtccgcagg agtttgctgc ggtcgatctt ggttcaaaca gttttcacat ggtcatagcc
     4981 cgtgtggtag atggtgccat gcagattatt ggccgcctga aacagcgggt gcatctggcg
     5041 gacggcctgg ggccagataa tatgttgagt gaagaggcaa tgacgcgcgg tttgaactgt
     5101 ctgtcgctgt ttgccgaacg gctacaaggg ttttctcctg ccagcgtctg tatagtcggc
     5161 acccatacgc tgcgtcaggc gctgaacgcc actgactttc tgaaacgcgc ggaaaaggtc
     5221 attccctacc cgattgaaat tatttccggt aatgaagaag cccgtctgat ttttatgggc
     5281 gtggaacata cccaaccgga aaaaggtcgc aaactggtta ttgatattgg cggcggatct
     5341 acggaactgg tgattggtga aaatttcgaa cctattctcg ttgaaagccg ccggatgggt
     5401 tgtgtcagct ttgcccagct ttattttcct ggcggggtca tcaataaaga gaattttcag
     5461 cgcgctcgca tggcggcagc acaaaaactg gaaactttaa cctggcaatt ccgtattcag
     5521 ggctggaacg ttgcaatggg cgcttccggt accataaaag ccgcccatga agtgttaatg
     5581 gaaatgggcg agaaagacgg gataattacc ccggaacgtc tggaaaaact ggtaaaagaa
     5641 attttacgtc accgtaattt cgcatcgctg agtttaccgg gtctttccga agagcggaaa
     5701 acagtcttcg ttcctggact ggcgatttta tgtggtgtgt ttgatgcttt agccatccgt
     5761 gaactgcgcc tttctgacgg ggcgttacgc gaaggcgtac tgtatgaaat ggaaggacgt
     5821 ttccgtcatc aggatgtgcg tagtcgcacc gccagcagcc tcgccaacca gtatcacata
     5881 gacagcgaac aggcccgacg agtgctggat accactatgc aaatgtacga acagtggcgg
     5941 gaacagcaac cgaagctggc gcatccgcaa ctggaggcgc tactgcgatg ggccgccatg
     6001 ctgcatgagg tcgggttgaa tatcaaccac agcgg
//
LOCUS       DAJTUN010000170         6033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTUN010000170 DAJTUN010000000
VERSION     DAJTUN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.171"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..735)
                     /gene="torZ"
                     /locus_tag="OO470_003610"
     CDS             complement(<1..735)
                     /gene="torZ"
                     /locus_tag="OO470_003610"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6252723.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKP"
     gene            complement(760..1860)
                     /locus_tag="OO470_003611"
     CDS             complement(760..1860)
                     /locus_tag="OO470_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6252724.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(2248..2994)
                     /gene="cutC"
                     /locus_tag="OO470_003612"
     CDS             complement(2248..2994)
                     /gene="cutC"
                     /locus_tag="OO470_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6252725.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(3008..3574)
                     /locus_tag="OO470_003613"
     CDS             complement(3008..3574)
                     /locus_tag="OO470_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6252726.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3790..5523
                     /gene="argS"
                     /locus_tag="OO470_003614"
     CDS             3790..5523
                     /gene="argS"
                     /locus_tag="OO470_003614"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6252727.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            5700..>6033
                     /locus_tag="OO470_003615"
     CDS             5700..>6033
                     /locus_tag="OO470_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6252728.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECEL"
BASE COUNT         1545 a         1528 c         1432 g         1528 t
ORIGIN      
        1 tggtttgcca gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct
       61 ccaggcaatt tttaaggtat tcagcgggtt cattccccac agcaccacaa cctggctgtt
      121 ttccagaatc agcggccagg aggcctgctg ttcatacacc tctacagaac cgaccacatg
      181 cggcatgatc acctgtgctg ccccggttga ataatcgccg ctatgcccgg aataaccgcc
      241 cgccaggttc atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg
      301 ccagccgtaa gatccggcaa aaatggccga tggtccgtta gctttacgaa tacgctcatg
      361 ttgctcatga atcagtttta atgcctgttc ccagctcacc tgtacatagg tatcttcgcc
      421 acgacctttc gccggttgca gtggattgtc gagatagctt tttctcacca tcggatgctg
      481 aatacgcgcc gtggtgtgta cctgatccgc cgccgtagac tgtaaggagt tcggtatggt
      541 tttcgccagc gcgcctgtcg aagaaacaat cttgccgtcc ttcacttcta cgctcatcgc
      601 tccccaacgt cccgcggtga ggattttacc gcccttctct tctgcccatg cggggagcgg
      661 tgctgccgat gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa taaattcacg
      721 tcttgttaat gccataactt cctccctgat caacgaggat cactgtttct cggtaatatc
      781 tttggcgttg tactggaaat accgcgttaa aatgtccggt tcgttttcgc tcatgctggt
      841 tcgtgccccc attcctttgg caatggacgg ccacgcattg acggtgtaat ggtcggcggc
      901 aatagaggca tgacaaccag cgcaataggt atcgtcaagt ttttcagcgt attgccatag
      961 cggtttacgg tccgctaatg cgggatcggt aagcgcaccc tgtaaagacg cctgacgcca
     1021 ttgattgccg tattcgtcag cctgccattc cccgtttacc gtgagcgcct tgataccttc
     1081 ttcacttaat gtggctagcg ccagccgttg acctgccgcc aggtagagcg tgttttcact
     1141 gccctgcatt tgataaccct gcaacagaac gatcggctgt ttgccactgg catcaacgac
     1201 ggtgagatcg gtaccaggat tcacggtagc cagctcgcct atgcgagaag ttttgaaagg
     1261 ataaatatgt gcgccattag tcactgaagt agcggcctga ctttccagct catgcgccgc
     1321 gttgtcatcc atttttattt ctggcggaaa atgggcaatg cctttatgac aatcgataca
     1381 ggtttcgccg cccttttgtg ctttgttatg cattttctgc gcagattcac tttgcgaggc
     1441 aatatccatg gcatcaaaag aatggcaact acggcacgtt gcagagtcag ttgcttttaa
     1501 ttctttccat actgtttcgg ccatttcctg gcgatgagtt tcgaacttat cgtcactgtc
     1561 tattttgccg ctaacaaatt catgataaat atctttagat gcttttaatt tagcaaataa
     1621 ataatccatc cctgactttg gaatatggca atcggcacat tccgcacgta tccctttctg
     1681 gttcgaaaag tggacagttc cctgatattc ctcaaaaggt ttactcatcg agtggcagga
     1741 aaggcaaaat gctgtatccg acgttttatg taaggctttt tgcgccagca ataacccgcc
     1801 gccaccaacc acaaccgcta tcagcaaaaa caataaccca atgcgttttt tccctcgtat
     1861 agattcctct aaataaattc cagaacaccg aaatcattgt ataattaaat acacaacgaa
     1921 ttaaccgctt gcataactcg gcacaacact gcctgaaata atcgataaag aatatgattt
     1981 attacaatgt aatcattaat tgctaaggaa taacccagtt gctatttaga atatatattt
     2041 atcagtttta gcaatttaaa tcccataatc aatgtgaata taaaaaatat ttttagcaca
     2101 acgctatgcg cactgtcacc cgctcaacag atcacggtta tcgttcgttt tttatactgc
     2161 tcagggataa aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata
     2221 ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt
     2281 catttcagca accgccgccc cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc
     2341 tgatgacatg gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc
     2401 ggagctatgg acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac
     2461 tcctgctcca gccataatga ttggagcatc acgatgggca ataagttcca taatttttga
     2521 taaaccttgc agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc
     2581 cgcaagatta ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt
     2641 cactgccagc ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc
     2701 gtcaacatcg agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac
     2761 atcctcaaga atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg
     2821 aattatcgga tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cccccaacga
     2881 cggcgttaag cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg
     2941 ctgcgccgtt agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc
     3001 tccttaatta cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact
     3061 taatagttgt tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac
     3121 cttttggtga actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca
     3181 atgccagcgc acgggcattc agcgtttctg gatcacccgg cagaacgatt tcaatatgtt
     3241 cccaaccttc gtgtgggtaa cgtttttccc cgggccacgg tagctccaca ttggaaaact
     3301 gccagtgcgc aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga
     3361 tcattttttc tgacaaaagc tcgccacact gttcaaaacc gcgacgccag cgctcagcgg
     3421 tgacgttttg atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atatccaacc
     3481 ccagacggcg ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat
     3541 cctgcagctc gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag
     3601 cgttaattta ccctgttgcc ctgtgccaac caaccgctga tttcacgccg cttctgatgc
     3661 aatagtgaaa acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta
     3721 tactcccgcc ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa
     3781 ggtattccgg tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg
     3841 gcaggcgcgc ctgcggattg cgaaccgcag attcgtcagt cagcaaaagt tcagttcggc
     3901 gactatcagg ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa
     3961 ttagcagagc aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc
     4021 gccggtccag gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag
     4081 caggcgctgg cgtccgatcg tctcggtgtt gctacgccag aaaaacagac cattgtggtt
     4141 gactactctg cgccaaacgt ggcgaaagag atgcacgtcg gtcacctgcg ctctaccatt
     4201 attggtgaca cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac
     4261 cacgtcggcg actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag
     4321 caggaaaacg ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa
     4381 aagcattacg acgaagatga agagtttgcc gagcgtgcac gtaactacgt ggtaaaactg
     4441 caaagcggtg acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg
     4501 cagaaccaga tcacttacga tcgtctcaac gtgacgctga cccgtgatga cgtgatgggc
     4561 gaaagcctct acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg
     4621 gcagtagaaa gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa caaggaaggc
     4681 gaaccgatgg gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat
     4741 atcgcctgtg cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct gtattacatc
     4801 gactcccgtc agcatcaaca cctgatgcag gcatgggcga tcgtccgtaa agcaggctat
     4861 gtaccggaat ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc
     4921 aaaccgttca aaacccgcgc gggtggtacc gtgaaactgg cagacctgct ggatgaagcc
     4981 ctggagcgtg cacgtcgtct ggtggcagaa aagaacccgg atatgccagc tgacgagctg
     5041 gaaaaactgg ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac
     5101 cgcaccacgg actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg
     5161 ccatacatgc agtatgctta cacgcgtgta ttgtccgtgt tccgtaaagc agaaattaac
     5221 gaagagcaac tggctgcagc tccggtaatc atccgtgaag atcgtgaagc gcaactggca
     5281 gctcgcctgc tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat
     5341 gtaatgtgtg cttacctgta cgatctggcc ggtctgttct ctggcttcta cgagcactgc
     5401 ccgatcctca gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg
     5461 acagcgaaga cgctgaagct gggtctggat acgctgggta ttgagactgt agagcgtatg
     5521 taatcgattt ttcgtgagag tgaagcctga tcagggttag ccgatcaggc ttttttattg
     5581 ccatctaaat gtattctgaa aacggacatg ccattgtttt ctcactgttg agtaagaggc
     5641 cagaagcgta atatccaggc ccaggaaaac gataacggtt caatttaagg aataccgaag
     5701 tgtttaaatt tcttgtatta acattaggca ttatctcttg ccaggcttac gcagaagata
     5761 cggttatagt aaacgaccat gacacttcag ccatcaaaga ttgttggcaa aaaaattcag
     5821 atgatgatac tgacattaac gtgatcaaat catgcctgcg acaagaatac aatctcgtcg
     5881 atgcgcaatt aaataaagcc tatggtgaag cttatcgtta tatagaacaa gtgccacgca
     5941 caggtgcaaa aaaacttgat actgaacaac ttaacttgct taaaaaatca cagcgagcct
     6001 ggctggattt tagggacaaa gaatgtgaat taa
//
LOCUS       DAJTUN010000171         5984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTUN010000171 DAJTUN010000000
VERSION     DAJTUN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.172"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..263)
                     /locus_tag="OO470_003616"
     CDS             complement(<1..263)
                     /locus_tag="OO470_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6252729.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            484..912
                     /gene="uspG"
                     /locus_tag="OO470_003617"
     CDS             484..912
                     /gene="uspG"
                     /locus_tag="OO470_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6252730.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OO470_003618"
     CDS             complement(1033..2598)
                     /gene="ahpF"
                     /locus_tag="OO470_003618"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6252731.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OO470_003619"
     CDS             complement(2843..3406)
                     /gene="ahpC"
                     /locus_tag="OO470_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6252732.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3778..4524
                     /gene="dsbG"
                     /locus_tag="OO470_003620"
     CDS             3778..4524
                     /gene="dsbG"
                     /locus_tag="OO470_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6252733.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4734..5636
                     /locus_tag="OO470_003621"
     CDS             4734..5636
                     /locus_tag="OO470_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6252734.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5783..5984
                     /locus_tag="OO470_003622"
                     /pseudo
     CDS             5783..5984
                     /locus_tag="OO470_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1606 a         1441 c         1348 g         1589 t
ORIGIN      
        1 atgacgaacg gaattaccac ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt
       61 tcaacgactt cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt
      121 ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttaccgtaat acgcagaata
      181 atatcatctg cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg
      241 ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt
      301 taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctag
      361 tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat
      421 atcagggata aatattataa tcatggagaa tgatttgttc atgattaaca gggagaaagg
      481 gttatgtata aggcaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa
      541 gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
      601 ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa
      661 gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc
      721 gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag
      781 ttggcagaag aactgggggc tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg
      841 acccatctgt taggttctaa cgcctcgagc gtgatccgcc acgccaatct gcctgtactg
      901 gtcgttcgct aacagataaa agtatttatg ccgccctgcc tggaacatcc cggcagggct
      961 ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa
     1021 tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc
     1081 tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc
     1141 cgctgcgaac acgcctttga cgttggtttc acatttcgca tcaatgataa tctcgcccat
     1201 gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac
     1261 aaaaataccg gccagttcga tgttgtgaat attgccgctg acacgatctc gatattccag
     1321 accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc
     1381 gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc
     1441 aaattccagc agcgttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc
     1501 ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcggtc cgtcgcagtg
     1561 cgggcagtag gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg
     1621 ccattttgca cccgtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt
     1681 ttcaatctgg tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct
     1741 gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccaacttctg
     1801 cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc
     1861 accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc
     1921 cgctgcaccc gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag
     1981 ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt
     2041 catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt
     2101 gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg
     2161 gttcaatacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag
     2221 cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga
     2281 ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa
     2341 ctcgtggccc agcggggagc ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat
     2401 caggaaagac ggcttacgca ctggcaagct gttatcttct ttaaaggtga ctttgtctga
     2461 cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag
     2521 cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt
     2581 catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc
     2641 tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca
     2701 cccgaagaat taggtgaatt tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg
     2761 aatccccggg agcttacaca agtaagtgac cggggtgagc gggcgacgcc aacgccgcta
     2821 tggcgtgaaa gacgaaggaa atttagattt taccaaccag gtccagagac ggagccagag
     2881 ttgcttcacc ttctttccat ttagccgggc aaacttcacc tgggtgagaa gctacgtact
     2941 gagctgcttt gattttacgc agcagatcag acgcgtcacg gccaatgcct tcagcggtaa
     3001 cttcgattgc ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac
     3061 cttcatcttc acgcatgttg tcgaagttac gggtcagggc gccagtcggg tcgccgatca
     3121 tcgcatattt gattttagcg atggtttcag agctgctgtg ccatgctttg tgggtgaagt
     3181 gagtatcggt agatactgcg tatacgtcta cgcccagttt ctgcagttct tcgtagtggt
     3241 cagcaacgtc acccagttcg gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga
     3301 agacgctcca gcggccttcg gtatcttttt cggtgatttc gatgaattcg ccgtttttga
     3361 atgcctggtt tttaaaaggt ttaattttgg tgttaatcaa ggacatctat acttcctccg
     3421 tgttttcgat gagatgtaag gtaaccaatt tttgccgatt cggctaatgc gtttccatta
     3481 tcaaatcgat aagttttacc ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc
     3541 cagcggcgat aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc tgaactaccc
     3601 tttggtatct tcagcgcctg cctgcaggct gaggtgttgc ccttacaaat gcaacaacga
     3661 catggattac aacaccctca taaacaaagg gcaatcacct gatctaagct cttacctatg
     3721 acagtgatag gttatgcctt ttactcgact tttgcactga ctgaaaagaa caaattaatg
     3781 ttaaaaaaga tacttttact ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct
     3841 tccccagtaa aagcgattga aaaacagggc attacaatca tcaaaacgtt cgatgccccc
     3901 ggaggaatga aaggttatct cggaaagtat caggatatgg gcgtcaccat ctacctgact
     3961 cccgatggta agcacgctat ctctggttac atgtacaacg agaaaggtga aaacctgagt
     4021 aacacactta tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg gcaacggatg
     4081 gaacaatccc actggctcct cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc
     4141 tccgatccgt tctgcccata ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat
     4201 tctggcaaag tgcaattaag aacattgttg gttggggtta tcaagccaga aagcccggcg
     4261 acagcagcgg caattcttgc ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc
     4321 tctggttgca agcttaagct aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg
     4381 ttaagtgaca atgagaaact gatggacgat ctgggggcaa atgtcacgcc ggctatctat
     4441 tacatgagta aggaaaatac gctgcaacag gccgtggggt tgcccgatca gaaaacgctt
     4501 aatatcatta tgggaaataa ataaaaggta attcatggat ggttgaacta tatccctgct
     4561 tttcaaccat cctaaaaaag catgaatttc tcgctctacc ctaagtggct gtgctatttt
     4621 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatac atacgcaaaa
     4681 aacattgact aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca
     4741 atctctacga cttgaaaaag ttcgacttaa atcttcttgt catatttgag tgtatttacc
     4801 agcatttaag cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc
     4861 agtcgttaca acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag
     4921 gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag
     4981 gccttgaaca aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct
     5041 acggccctca gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag
     5101 atgcctctgt cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac
     5161 tgttggttca ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg
     5221 tcatttgcat gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc
     5281 gaattactga caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatag
     5341 ccaaatcagc aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca
     5401 aaatttcatt tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt
     5461 tactgggaat agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag
     5521 agataaaact agaacatccg ttaccttcaa taaagcttta catttcctat aataaatctt
     5581 ctttaaataa tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata
     5641 taactataga cagctactat aactttcatc tatttattca cagcgcgaat gaaaaataaa
     5701 acactctgct ttacagtgtt tactttataa ttaacagtct tatctcactg ttcaccctgt
     5761 aacgttcaga gagcgtcttc agatgtctat ttataaaatt cctcttccgc tcaatattct
     5821 ggaagctgca cgagagcgta ttacctagac actcaacacc ctaccccgcg tttgtgtctc
     5881 attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca
     5941 aatgggcaaa aaaatctgcg ttttgtttat caactgggag gcgc
//
LOCUS       DAJTUN010000172         5915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTUN010000172 DAJTUN010000000
VERSION     DAJTUN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.173"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(54..317)
                     /locus_tag="OO470_003623"
     CDS             complement(54..317)
                     /locus_tag="OO470_003623"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6252735.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            249..755
                     /locus_tag="OO470_003624"
     CDS             249..755
                     /locus_tag="OO470_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6252736.1"
                     /translation="MRFSEIAAVYLHAFNKMLVSGTHTQWKLIDLQSDLFTVVFDSVV
                     HTAKHNKIVISAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSCHEHCY
                     AQIMVGRAGTSQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTLPTLSQ
                     PEIAIVDG"
     gene            771..1265
                     /locus_tag="OO470_003625"
     CDS             771..1265
                     /locus_tag="OO470_003625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6252737.1"
                     /translation="MAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILVRQPNG
                     GTRKLPCYQITRGGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADMSSVAQ
                     NARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRLNGSDK
                     EVRP"
     gene            1262..1495
                     /locus_tag="OO470_003626"
     CDS             1262..1495
                     /locus_tag="OO470_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252738.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            1482..2390
                     /locus_tag="OO470_003627"
     CDS             1482..2390
                     /locus_tag="OO470_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6252739.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            2401..3279
                     /locus_tag="OO470_003628"
     CDS             2401..3279
                     /locus_tag="OO470_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6252740.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            3276..4667
                     /locus_tag="OO470_003629"
     CDS             3276..4667
                     /locus_tag="OO470_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6252741.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            4664..4921
                     /locus_tag="OO470_003630"
     CDS             4664..4921
                     /locus_tag="OO470_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252742.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            5016..5216
                     /locus_tag="OO470_003631"
     CDS             5016..5216
                     /locus_tag="OO470_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6252743.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            5209..5589
                     /locus_tag="OO470_003632"
     CDS             5209..5589
                     /locus_tag="OO470_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6252744.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            5789..5865
                     /locus_tag="OO470_003633"
     tRNA            5789..5865
                     /locus_tag="OO470_003633"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5823..5825,aa:Ile,seq:cat)
BASE COUNT         1506 a         1375 c         1607 g         1427 t
ORIGIN      
        1 cttccatcca tttggctgcc atttcgtcgt gtgtcatggt ggggagagtg cgtttaccag
       61 tcatgcttaa cctccttcat tctggtttca gctttcttgc gttcggctgg cggtgttttc
      121 tgcgctttct ttacaaaact atgcagcatg atgatgcgtt tccctgtcag agtgcagtaa
      181 aaaacacgcg cgatcccatc gttgccttta attctgagtt cgaaaagccc gtcaccaaag
      241 gcgctggtgt gaggttctcc gagattgctg ccgtatacct tcatgcgttc aacaagatgc
      301 tggtatctgg cacgcatact caatggaagc tgatcgacct ccagtcggac ctcttcactg
      361 tagtattcga tagtgtagtt catacagcaa aacataacaa aatcgttata tctgcacaat
      421 atattgattc tgcaatttcg ggacgttaca ctgtctctgc accttataaa gcgggggccg
      481 ggattggcgt cctgaaattc aatatagagc ataaccgcgc tcatgcggtt ttttcgtgtc
      541 atgagcattg ctacgcccaa attatggtgg ggcgtgcagg gacatcgcaa gatgcgccgg
      601 gttctatgtt gaccggttac gccaaccctg tacgtctcac cacctctgtg attggcgtcc
      661 catgtggtga gttctttgaa ttcaacatag gggctgtcac catgactact ctcccaaccc
      721 tctctcaacc tgaaattgcc atcgttgatg gttaggctgt tacttcatcc ctggctgttg
      781 ccaacttctt ctctaaacgt catgacgatg ttctgaaaaa gatccgcatt ttggattgtt
      841 ctccagagtt ttgtgcccgc aattttgcgg agacatcaat tttggtacgc cagcccaacg
      901 gcggtactcg caaacttccc tgctaccaaa ttacccgcgg cggcttcgcc ttccttgcta
      961 tgggcttcac tggtaaacgt gccgcccggt tcaaagaggc atacatcaat gcctttaacc
     1021 agatggagag gagcttatca ggagctggtg cggctgacat gtcatctgtc gcacaaaacg
     1081 ccagaggcgt atacctgcat ttgcgtgaaa tccatcaaat ctggacaagc cagctttacc
     1141 caatgcttaa ggccgttgaa tctccgctgg ctggcaaact gtacgaccgt gtaggtgatg
     1201 ctgtttttgg tgctgcactt gttgattcca ggctgaatgg ttctgacaag gaggtgcgcc
     1261 catgatccgc cacatcgtta atttcctgta tcaccgatac aaccattgcc cccgtgtggg
     1321 gcagtggttc accaccagca acggctacgt tctgcgggtt tgcctggtca gtaccgaaag
     1381 ccagaaggtt gtctgccagg ttcagggacg tactcatacc ctgagttatc cgctggtggc
     1441 gtttcagccc ggaaaaatgt ttaaacgcct gggaggtggc tatgcgtccg tctgatcttc
     1501 tgctcgattt tggacatccg gttgcttatt accctgggct cgttaaatac atgggaagtc
     1561 cgcacgctgt tattttcttt ggtcagattt tttactggca ggataaagca catgcagcgg
     1621 aaggcgtaca taaaacgcgt gaagagatac aacacgaaac cggacttaca tttgaacaac
     1681 aggctgtagc gcgtaagcat cttgtgtcca gaggcatttt ggttgaaacc aacaagcgtc
     1741 ttgagcacaa aatgttctac cgtatagatt gtgagcgcct taatgaaatt atcaatgaaa
     1801 acaatcagtt ttcccgaaat ggggaaaccc gttttcggga aactgtaaaa cccaatttcg
     1861 cggaggaggg aaagccttca ccgcggacac gggaaacccc tcgccgcgga gaagggaaaa
     1921 ccaatttcga tcttacagag aatacaacag agattacttc agagaatact acagagagta
     1981 aaaacactat tggcgcatcc gctgacgcgt ctgcaccagc gcgttcttcc cgacaggaat
     2041 attcaccgga atttgaacag gcctggcagg aatatccaaa acgtgctggt ggtaactcaa
     2101 aatccgcagc cttcaaagcc tggaaagccc ggctcaggga gggaataaaa cctgaagcca
     2161 tgcttgatgg cgtgaaacgc tatgctgcct gggtacgtgc cacaggaaat accggcacac
     2221 agttcgtgaa gcaggctgcg acgttctttg gacccgatcg tcacttcgaa gacttctggc
     2281 aacagccagc cgctcccaga ggtgggcgac agcgacaggt cgatgtcctg gctggcctgg
     2341 gagccatgtc tgacaaattc ggtaaatcca gtgacaaact gacattctga ggtgacagcg
     2401 atgatgacgt ttaacctgcg tgaacaacaa aaaagactac aggcgcgaat ggatgagtta
     2461 cgggcagaga ttgcatttgc tcagaagggc gaaaagccat ggccttatcg ttcctgcctg
     2521 atgcgtgaag gtcgcggata ttgcgaaaaa catggcgaat atcacacgca tatactggtg
     2581 tggagcgatc gtaatggcga ggacagagaa gaaatttcat gctgccctga ctgcttaatc
     2641 gctgaggcca acgatttgac catggagctg tcgtccatca aggcggaaga gctgactgat
     2701 aacgccggaa ttgccctgcg ttttcgggac tgcgagtttg ataattatct ggaggttaat
     2761 cctggcgcag ccagaaatct tgcggcctgt cgccgctatg cggagaactg gccagatatg
     2821 ctggagaacg gtaccagtct tgttatgacc ggcagttgtg gtaccggaaa aaatcatctg
     2881 gctgtggcta tggcaaaaca catcatccgt aactatctgg ccagtgtgga gatcaccgac
     2941 gtgatgcgcc ttacccgtgc tgtgaaaaat tgctggcgga atgacagcga aaaaacagcg
     3001 gatgaagtta ttgagcgtta tgcgtcaatg gatttgctga tcatcgacga agttggcgtt
     3061 cagtttggca gcgcggctga aatggctatt ttgcaggaaa ttatcaacgc caggtacgaa
     3121 agtatcttgc ccaccattct tatcagtaac ctctcaccgg aagagttgtg ggcgttcatc
     3181 agtccccgaa ttgccgacag gatcacagac gggggacgca actggttgtc gtttaactgg
     3241 cccagttacc gttctcgtat cagaggtgtg gctgcatgac aactccagta tggcgtaacg
     3301 atgaccttga aggcgctgtc atcggcgcgt tctttctgcg cggagcagat cctgaagtga
     3361 tggatattct ggccacactg ccagcggacg ttttttctgt acgagcgtat caggatatct
     3421 atacaggcat ctgcagacag gcccgtgttt caggagtgat tgaccccgtg ctgttgtgta
     3481 atgagatgcc ggaacttgcc ccggtgatta ctgataccgg gcgtaaaacc tgggtgaagt
     3541 cttcactgga gcactatgtt gcagcgttgc ggcgcaatgc cgcactgcgc gatgcagaaa
     3601 aaacactgaa tgaggcgctg cagaaattac gtgatgcgca tacctgtgaa gcagctgaag
     3661 atgccctgaa ggatgcgcag aacatgatgg tcacactgtc gacaggaaag ggcgtcattc
     3721 agccggtaca tattgatgat gtgcttccgg aagtggttga gcgtgttgaa tgccggaatc
     3781 aggggctgga gaaatccagg acgttgatga ccggtattga tgaactggac gcaaaaacag
     3841 gcggtatgga gcccggagac ctggtattca ttgccgcccg tccttcgatg gggaaaaccg
     3901 aacttgcgct ggacatcatc gacaaggtga ctgagcaggg gcatggtgtg cttctgttca
     3961 ccatggagat ggcgaacatc cagattggtg aacgtatggt gtctgcggct ggagggatgc
     4021 cagtatcacg cctgaaatct gtggctcact ttgaagatga agactggacg cgtttctcac
     4081 aaggggtggg gcggatgacc gggcgcaata tctggatggt ggaccaggcg aacctggcca
     4141 ttgatgagat atgcgcaaca acgaaacacc acctgattaa atatccggaa acggcgctgg
     4201 tggtggttga ttatctcggg ctgataaaaa cccgaaccac ggggcgtcat gaccttgccg
     4261 tgggtgaaat ctcaaagggg cttaaaggcc tggcaaaatc cggtggtttt ccgttgattg
     4321 cgctgagcca gctctcccgc ggtgtggagt ccagacccaa taaacgtccc atgaactcag
     4381 acctgaaaaa ttccggagaa atagaggcgg atgcagacat cattctgatg ctttacaggg
     4441 atgaagtgta caacccggat acgcaggcca ggggcatcgc agaaatcaat atcacgaaac
     4501 aacgtaacgg ttctctggga acgatttacc ggcgttttta taacggacat tttctgcctg
     4561 tggaccagga aagtgcacgg gtgctttcca ctcccatgaa gcccggcaat ccgcgcagat
     4621 acagtaataa acgaactgac agcagtaaga tggagcgttt cttttgaaca accagacaat
     4681 gacttttacc cctgaacaat tgcgtaaaca cgcgcaggaa atgctgcgtc atgctgaaca
     4741 actcgaaaaa acgggcataa caaaagatgc tatccgtaaa gatatggtac ccgcgcttcg
     4801 ggaactgatg caggcgaagc atcgcgcaca gaaagcagta gatgagctgg tggactgtgt
     4861 ggcagagctg gaaaccagag tcggaaagtt tgaaaaactg gtgcaggagg cgctgcgctg
     4921 atgcgccatg agtttgtttt gccctggctt gtgcgcgatc attatgtccc tggtggaaag
     4981 cccgggataa aaatcaccga accggagagt atttgatgag cgtcaaaatt caaacgatac
     5041 cggaactgct tatccagaca cgaggtaata tgaccgaagt ttcacgaatg ctgaactgta
     5101 atcgcgccac ggtaagaaaa tatgctgaag ataaagaggg caaagggcac gccattgttg
     5161 atggtgttct gattgttcat cgtggatggg acaggggtaa agacagtgat gcgtgatatt
     5221 cagcaggttc ttgagcgctg gggcgcgtgg gtggctaata atcatgagga tgtgacctgg
     5281 tcgtccattg ctgccggttt taagggatta atcccttcaa aagtaaaatc ccgcccgcaa
     5341 tgctgtgacg atgacgcgat ggttatatgt gggtgtatgg cccgccttaa ccggaacaac
     5401 aacgatctac atgacttgct ggttgactac tacgtgtttg ggatgacatt catgacgctg
     5461 gcgcggaagc atggatgctc agatggttat ataggaaaga agctacaaaa agccgagggg
     5521 gtggtggaag gaatgttgat gatgttgggt gtccggttag aaatggacaa atatattgaa
     5581 catccctgat ggtaaggggc gactttctag aaaatatttt acgtacgtaa aaaacgggat
     5641 attctgttaa gagtggttgc ttcgccacac agcttaaacc cgcctccggg cgggtttttt
     5701 atgcctgaaa aacggtacag gacgttaaac acgctggtgg tcagatgagt ttgcagatgt
     5761 gatgacatat ggttattatt ctgcctccgg ccctttagct cagttggtca gagcgagcga
     5821 ctcataatcg ccaggtcgct ggttcaagtc cagcaagggc caccaaccac cactagctca
     5881 tccggataga gcatcaacct tctaagttga cggtg
//
LOCUS       DAJTUN010000173         5770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTUN010000173 DAJTUN010000000
VERSION     DAJTUN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.174"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..931)
                     /gene="hprS"
                     /locus_tag="OO470_003634"
     CDS             complement(<1..931)
                     /gene="hprS"
                     /locus_tag="OO470_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6252745.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSL"
     gene            complement(931..1602)
                     /gene="hprR"
                     /locus_tag="OO470_003635"
     CDS             complement(931..1602)
                     /gene="hprR"
                     /locus_tag="OO470_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6252746.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1735..2148
                     /gene="hiuH"
                     /locus_tag="OO470_003636"
     CDS             1735..2148
                     /gene="hiuH"
                     /locus_tag="OO470_003636"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6252747.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2257..3261
                     /gene="msrP"
                     /locus_tag="OO470_003637"
                     /pseudo
     CDS             2257..3261
                     /gene="msrP"
                     /locus_tag="OO470_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3262..3897
                     /gene="msrQ"
                     /locus_tag="OO470_003638"
     CDS             3262..3897
                     /gene="msrQ"
                     /locus_tag="OO470_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6252748.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4155..4805
                     /gene="zinT"
                     /locus_tag="OO470_003639"
     CDS             4155..4805
                     /gene="zinT"
                     /locus_tag="OO470_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6252749.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1639 a         1305 c         1193 g         1633 t
ORIGIN      
        1 aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga aacagtatat
       61 tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt gttttttgat
      121 attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt aatgcattaa
      181 ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc tcaaaatctt
      241 tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt tctctcggta
      301 atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc gttacgccac
      361 tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat actgagcaca
      421 gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca agcatattat
      481 gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact tcatcaatgt
      541 taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg cctgctgcgc
      601 tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt gtccggttaa
      661 caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca tccatcatcc
      721 ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac tgcttgatct
      781 gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt gccaggccat
      841 tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata aaaagcaagg
      901 ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga atgaatagcc
      961 catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt ttgcgcggag
     1021 cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc cccaaatttc
     1081 actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta gccaaagtaa
     1141 ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac tttgactgac
     1201 agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat gttgccttaa
     1261 ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca gataatcatt
     1321 tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag taaggcaaat
     1381 aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat ccatacccgg
     1441 aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat aaagcccatc
     1501 tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct gcgttaccca
     1561 ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa tatgccataa
     1621 atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct gtaaggttac
     1681 cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat aaacatgtta
     1741 aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc tttggtttat
     1801 gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg aaaacccgct
     1861 gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca acttaatacc
     1921 gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac tgcgactact
     1981 ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa tcttgaaagt
     2041 ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca ttatcatgtg
     2101 cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg cgtaaatatg
     2161 taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa cagtaaacag
     2221 ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca atttttaaaa
     2281 gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt gttaaaagca
     2341 ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga tctgcttagc
     2401 tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga gttcagcaag
     2461 cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc cggttataac
     2521 aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg tagcctgaaa
     2581 accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac cctcgatcat
     2641 gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg ctgcgtggaa
     2701 gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact ggcgcttgcc
     2761 gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc ggaacagatg
     2821 cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt cgaaggattg
     2881 cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta tggcaaggcg
     2941 ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata tggctttaaa
     3001 gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac cacctggaat
     3061 ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt tgatcacccg
     3121 cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga tgttcagcgc
     3181 cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta tcgtggcctg
     3241 gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg gctgaaagtt
     3301 tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat caatcacggt
     3361 ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac tgcactgaaa
     3421 tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca gccgttattg
     3481 atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact gcatttaacc
     3541 agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa ggagttaatt
     3601 acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc tttagcgttc
     3661 acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt gcataacttc
     3721 gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa gattatttca
     3781 ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg gtataagaag
     3841 ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc tctataactt
     3901 catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc cctatccggc
     3961 ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca ccagaataat
     4021 ttattttatt tcaataagat agttcaatac acctgagaat accatgctct catttcctaa
     4081 tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt acattaactc
     4141 tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta ggtgtcttta
     4201 ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc ttaacagagg
     4261 tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac cgaacgctca
     4321 gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc aaacttgatc
     4381 ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa attaaagatt
     4441 attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac ggcattgtcg
     4501 aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga tacaaaatac
     4561 tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa gatcctgaaa
     4621 gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga aaatcatctc
     4681 attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa atggaaaact
     4741 ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag atgatgtctc
     4801 attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta gtaacaacaa
     4861 taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg agtcgtgtct
     4921 tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata cacaactcag
     4981 ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg gtaaagctaa
     5041 ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta tgatacaaag
     5101 gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt gtcccgcggc
     5161 tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct ctacgcaact
     5221 tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa agaataatgg
     5281 tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt cctgtcagct
     5341 ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga cgatctaacc
     5401 cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag tcgggagata
     5461 agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt tgcccctact
     5521 ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa tttttcttgg
     5581 aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt aaagaagaat
     5641 attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg tgatttgccc
     5701 cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt gttgaataat
     5761 tccactgcag
//
LOCUS       DAJTUN010000174         5712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTUN010000174 DAJTUN010000000
VERSION     DAJTUN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.175"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(98..1855)
                     /locus_tag="OO470_003640"
     CDS             complement(98..1855)
                     /locus_tag="OO470_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6252750.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(1871..2755)
                     /locus_tag="OO470_003641"
     CDS             complement(1871..2755)
                     /locus_tag="OO470_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6252751.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2766..3371)
                     /locus_tag="OO470_003642"
     CDS             complement(2766..3371)
                     /locus_tag="OO470_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6252752.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(3542..>5712)
                     /locus_tag="OO470_003643"
     CDS             complement(3542..>5712)
                     /locus_tag="OO470_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR6252753.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1655 a         1431 c         1186 g         1440 t
ORIGIN      
        1 aaccgcggtg aactgattaa gagatccata ggtgaagaag aaaaacgggg ccagcaacaa
       61 taaccagcca gcgccttgta gcaatacgtt ccgacgttca gttatcacga cgcaccgcca
      121 tcgaaaccgt aaaaatgccc cacacatcaa tccgttgagt gcatttatca aatccggcat
      181 tacgcacgag cgaatccatc tccccttgcg aacgcacgcg cattacccac ggtttaccat
      241 ctttatgact ggttaacacc ccggcaatca gctccagttg tgggtgccac ggctgcccgg
      301 tgtagatgag aatgccaccc ggttcgatgg cattggctag acctgcgagt gagtttttta
      361 cctgctcatt ttcgggaaaa agctcatata ggccagagac aatcgccagc gtaggccgcg
      421 gagttaacgc gctgagttcc tccgggttaa acgcatcgcc ctgttcaaaa cgcacccgcc
      481 cagacattcc ccgttgggca atcatctctt gcccctgtgc aacatttaac tcactgtaat
      541 cacgtaacaa aatatcgctt acggcaggct cgtttgccag cgcatccagt acatagcgtc
      601 catgccctgc ggcaatgtca accacgcgga cggctaaacc tttggcgtgg agatcggcaa
      661 cggcttgttt aatcaatatt tgcaaatggg ttttgcgctg gcgaataccg cgccatccaa
      721 cactgttcag gtagattttg tcgattaaac gcccgaatgc gttactgcct tgcggttgat
      781 tttgatagac ataatccagg gaactgcctg aatcaaagcc ggtactcatt ccgagatgca
      841 ggcccgcaga gtgcgcaccg aacaatttca tcgcctttcg cataaagcga tacgctaaat
      901 caaccggcga taatggcacg ggtccaccag aaaggagccg ccatcgatcc gctgatggtc
      961 ctgtgcggtc ttcatgctga taatcaaatt tttgcgattt gttagcatat aagcggcaga
     1021 taaagctttg cattttttca aatgcctgcg ccctgttctc ttcacccaat gtgtcgtgat
     1081 aaaagcctgg cagcagatgc agctctttca gagggctacg taatctctga taaaaatcaa
     1141 tctgcggttg gcgatgcacc acatagtcat cacctgatat cagaagttgc gtggggagcg
     1201 taatcgccgc cgcatcgcta acaatacgtt cagacgtttt atagagatcg agcaagatat
     1261 tgacggcaat cgcccgtgtg atcagcggat cattattgaa actcgccacc cgttgccgat
     1321 cgtgggtcaa atagcgccct ttcacatagg aattaataaa aaatagacca cgcagacgat
     1381 gccataacgc cagcgcagga cgtgccagcg gcacatacaa tttaacctta aaggccggag
     1441 aagccagcac cagcccacga attgcaggtg cataatcatg aacccatgtg gcaacaagca
     1501 ctgcgccgac gctttgcgcg atcactacca cctcttccag tccgacctgg ctgtcgctga
     1561 cagcaaaacg gacaaattca tcgacatcct gcactgagcg cataagagac gggctataac
     1621 cacgcggccc cgaatttttt ccatgccccc gggcatccca ggcataaaaa gcagtatcag
     1681 gcatcgccag ttcatcaacg agatgttgta gacgaccaga atgttcatgc ccgcgatgaa
     1741 ataagacgat gacctttttc gcccctggct gtaaagtggg ccagtgccga taatacaacg
     1801 ctgtattatc actggtggta aaaaaatgtt ccccagggat gcgtgaattt tccattttct
     1861 ctattttcct tcagtaacag cagtagcgaa taaaataaaa aaataccgga gcggtgaaaa
     1921 ttaatgagtc aatcctgtca aggagtccgc catgtcctag taatagtttt ccactatctt
     1981 taacaccaat atctcgtttg atggctgaca tcactacatc gccgcaaaaa ccactaatac
     2041 caattaacaa acccgctaat aatgcctgta atgtatttag cggtgtcagc aacggtccaa
     2101 taatcagtga cgcaatcatg gtggtgatta cgccccccag cagaccttcc agcgtttttc
     2161 ccgggctgac tttggggacc acttttcttc tgccgcagga tttcccccat aaatactgtg
     2221 caatatcgtt tgactcggtt aacgccaaaa gaaataacac cagtaacgcc ccgccctgga
     2281 tatttatggt tggcaacatt aatagccagg cggcatgact caaggcaaaa acggtcatta
     2341 tccatcccca aaaaatggcg ctcacggtat gctgaaaacc ggaggggtca cccacaaaaa
     2401 ctcgccaggt ggctaatatc agaaacccag tcaaaggtat aaataacaga aacagctcaa
     2461 agcaattaaa accgataagc aaatagttaa gaggaataac ccaatataac caacgcgaaa
     2521 aatgtacgga tataagcgta caatattctt tcaatgccag aaaacttatc agcgtaaaaa
     2581 acgtcaacgt catccagcgt gggcctgaaa tcaccagtga aaaacaaatg acgatgcccc
     2641 accaggtacg aatacgtaat gtaacttcat ccccttttct ctcaggtaac cgccatagca
     2701 gaaaacggtt tatcagcttg ctaaaattaa caacgcgaaa agtgttgcca gagatttttc
     2761 cagcattatc tttcagcgct cataagaagc acactccgac aacgattaat cgcagtacag
     2821 ataaacagaa ttgaagcaat gctccataaa agattattcc attgcatcca ctgtggataa
     2881 atggcaacgg ccagacccca cagaccaaat attaacgcgc gatcactttt gccaaatgga
     2941 ccggcataac tgcgaacacc gttaatcgtc tgcgcgagta aaccgcaaaa ctcggtcaat
     3001 atggtgcaaa ataacatgag tatcacgagt gatgcgttac tttccggtaa aaataaaaac
     3061 ggtaaataga gcgcaatatc ggaaataaca tcgccagtct cattcaaaat cgcccccaga
     3121 cgtgtttgct gattgcactc acgcgccaac atgccatcca gcgcattgag cgccatacgg
     3181 ataaaaagca cgatgggcaa tagcaaaaag aggatgggtt gtgccaccaa caccaacagc
     3241 aatccggtaa aaagagaaag cgccagtgcc gtaagagtga tgtgattcgc tgtaacgtgg
     3301 tgcttataaa gccaaaacat cgtcggcctt aacagcgact gaaagagcgg ttttatctgg
     3361 tagagcgtca ttatcaatcc ttgataattc ggtttgcgct atggtgcatc aattcccctg
     3421 acggagatat cgcaacacgc cgcttcatta aaaattcaga atatatcaag gtattggggg
     3481 ctcactgtca ggaatgatta tccccaataa atcaagtagt taaaaattat tacgctgtta
     3541 ttcaatacaa ctcatcaagt ttcctcatcg cttgttcccg tattgcgcgc gtcttaccgt
     3601 taagcgccag ctggcggact aaatccagtt cagggttaac aagctctaac gccagcaaag
     3661 gatcacgttt cagcctcgcc ttcaatacat caactggaaa gcggggatta ttcatcgcca
     3721 caatccacca ctcgggatca atttctgccg ccagtgccgc agagggggtg tggcggttgc
     3781 taagtgcgcc gaacttgaaa aatgtgagat tgcttttcgc cagtgtactc caccatatac
     3841 gatccggttg ttttgccagt acatccagtt gtgctaacgc atcggggtcg agtgcataat
     3901 aaacacgtaa tgcttcatca acctgcgcac gggagctatt ttcctgcctc agaaaaaact
     3961 tcgcccaaat atcattgcgt ggcgtgtttt gctgtaattc cttgagaaca acagcgggta
     4021 aattatccca actaactaat gcactctcta aaaagtccca gtcttccaaa cgtatcaggg
     4081 cggcggcatt taatagcatc tggtcatcaa cttcgccgtg gctaatcagc tcagacaaaa
     4141 tcatatcagc gctggtttca tatgcatctg aatctgcttt cggattattt ttccgatgcg
     4201 ataaagcacg ttgccaaatt ttttgtttca tcgacgtgct cagatactga tttttcgcca
     4261 gattactcca cccctcagga tcgttgaaac gtgtcaataa ccgttgtaaa gcagcgtcag
     4321 agtgaattac ctgcatatcc cgacagcaaa agtaggcagg ggattttttc tccatccttt
     4381 taacaaaatt aaactgtaag ttatctggca atgctaaaaa taacacacgt attagatcat
     4441 cgtcattctc gaatttttgg tataaatagg ctgcaacttt gtcactctct tgtggcgtca
     4501 tccgcaataa ttgaccatgg tgtttcatct ctgccagctt gaacgctaag cttgtgacaa
     4561 cattgtttga tgaatcgttc gccaactgca ttgcctgatt gtaagtgagt tgtcctccag
     4621 caccgacctg acgaatagag gaatcatcac tgcggatata gccatcaata atggattcgg
     4681 gcaataacca cgcatcttta taaagctcct gaatgatcat atcccaatct cttttcgcca
     4741 gagtcatgac gatatccaga gggagatttt cacgtagcgc aagattcaac ggaccgacac
     4801 catcaggtag acgactcatt cgcgtcatta tttcgacaga gagcggttca cggtcgagca
     4861 accccagaac aacaggctgg taacccaatt gctgatcggt caactccaga atctcattgt
     4921 caggcatata tctcgccata ctttcaatcg tttgttcatc attgctatta ataagttgtt
     4981 tgcgtaggtt agctgaaatt cctttaagcc ttgggagagt tcgcattatg gcaagatcaa
     5041 atgtcgcaat atctcgtcct tcaaatgcag caacgttgcc ttcatccagc atttgcaatt
     5101 cctgcactaa ctcaggagaa ggcttagggc gaaacagcgc ttcttttttc atctgattac
     5161 agacattccc tgccatgcga atattgcttc gcttaaaaat ttctttcaga gcatcttcat
     5221 catttattag accccaataa tatgcatcag gatttattat catcagcgct tttttgacat
     5281 tttcagtgtc atttttgtca ctcgcacgga ccatgaattc actatttgtc gaagacgcaa
     5341 caacaggtgc aattatgtca ttgataaata ccccacgcgc attaatactc cccttaatcg
     5401 tagacggatt atcgtaatat gagtcgataa ataccagtcc acttgcattt atcgaaccgg
     5461 taattaacag tggtgaatcc gtaactatat tcgttgccgt cacgttaccg agaataaccc
     5521 atggattaga aagagagaaa gtggaaatat tacctttgac tgtcaaattg ccaagaacaa
     5581 tcaatttttg attactctct atattacctt ctaccactaa atcgccacga tgcactttcc
     5641 agtccgatgc agataaaccg aaggggacaa gattcacgct actgttgata gcttgttcaa
     5701 cttgttgttt at
//
LOCUS       DAJTUN010000175         5604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTUN010000175 DAJTUN010000000
VERSION     DAJTUN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.177"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            59..1385
                     /gene="pgaC"
                     /locus_tag="OO470_003644"
                     /pseudo
     CDS             59..1385
                     /gene="pgaC"
                     /locus_tag="OO470_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1387..1800
                     /gene="pgaD"
                     /locus_tag="OO470_003645"
                     /pseudo
     CDS             1387..1800
                     /gene="pgaD"
                     /locus_tag="OO470_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="OO470_003646"
     CDS             complement(1850..2638)
                     /gene="phoH"
                     /locus_tag="OO470_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6252754.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="OO470_003647"
     CDS             complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="OO470_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6252755.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4517..>5604)
                     /gene="efeO"
                     /locus_tag="OO470_003648"
     CDS             complement(4517..>5604)
                     /gene="efeO"
                     /locus_tag="OO470_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6252756.1"
                     /translation="AALFSSAFMANAADVPQVKVTVTDKQCEPMTITVNAGKTQFIIQ
                     NHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMTCGLLTNPKGKLIVK
                     GEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDAIKAGDIEKAKALYA
                     PTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHRLEKALFGDNTTKGM
                     DQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKISGEEDRYSHTDLWD
                     FQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKYRTKDGFETYDKLTD
                     ADRNALKGPITALAEDLAQLRGVLGLD"
BASE COUNT         1330 a         1460 c         1339 g         1475 t
ORIGIN      
        1 aaccaacctg aaatagacct tattcgtcct gagttttcaa cagcctggta tccgaaaaat
       61 gattaatcgc atcgtatcgt tttttatatt atgtctggtg ttatgcatac ccctatgcgt
      121 agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat
      181 gtccattatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc
      241 gtggggagaa aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat
      301 tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg
      361 ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat
      421 cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaaaca
      481 ggggaaagcc attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg
      541 cattgatggc gatgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt
      601 gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac gttctaccct
      661 ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca
      721 gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc
      781 cctggcagaa gtgggttact ggaatgacga tatgatcacc gaagatattg atattagctg
      841 gaagctgcag ttgaatcagt ggacgatttt tttacgagcc acgggcgctg tgctggatat
      901 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
      961 cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc
     1021 cgctgttttt tgaatactgc ctgacgacaa tatgggcctt cacctgcctg gtcggtttca
     1081 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     1141 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     1201 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     1261 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     1321 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc tgatcgcggg attctgagag
     1381 gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt actggttgat
     1441 tatgttgcca caaccatctt gtagacatta tttgctttgt tcatattctt attcgccatg
     1501 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     1561 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     1621 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     1681 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac
     1741 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     1801 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     1861 catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg aaccggacga
     1921 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     1981 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     2041 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     2101 caactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcggtt tccaccttcc
     2161 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcaagt
     2221 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa
     2281 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     2341 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     2401 tggcgaatat cagctgctta ctctcaatag cctgcagata gtgcaattgc gcttcatttc
     2461 gcgcaaggat gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     2521 tcatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     2581 gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg
     2641 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac
     2701 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     2761 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagaaagga agtgagaaaa
     2821 atacagagtg acgaagttgc taccggagga gaaatctccg cgagtggtgg agcgttgctt
     2881 aacaaaacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     2941 actgccggga acttccgctg ctacttaata agtacaacag aactcgcatt cattgcaaca
     3001 atatattcac ttatatttaa cgataaacac ccatttcggt gacattaatt tctactggaa
     3061 agatgacaga gtgatgacag tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg
     3121 ctggtaaaat gtgcctcgtg tttgccggat tcggcgcgaa cgccttaccc gacctacaag
     3181 tgattaataa tttgtgcaca aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc
     3241 gtcgttcaaa cctgcaatag cgcccttccc agataatcgt tcgcgtcctt cactcccggc
     3301 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     3361 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     3421 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3481 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3541 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3601 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3661 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3721 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3781 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     3841 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     3901 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggtt caccgtgtcc
     3961 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     4021 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     4081 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     4141 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     4201 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca aacgaaacaa ccgctcaaga
     4261 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     4321 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     4381 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     4441 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     4501 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     4561 ccagcgcagt aatcggccct ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg
     4621 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     4681 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     4741 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     4801 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     4861 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     4921 cggtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     4981 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
     5041 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatac
     5101 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     5161 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     5221 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     5281 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     5341 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
     5401 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
     5461 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
     5521 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
     5581 aagcagaaga aaacagcgca gcca
//
LOCUS       DAJTUN010000176         5600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTUN010000176 DAJTUN010000000
VERSION     DAJTUN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.178"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..177
                     /locus_tag="OO470_003649"
     CDS             <1..177
                     /locus_tag="OO470_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6252757.1"
                     /translation="VMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFM
                     QLQLDERTEPDAIG"
     gene            174..623
                     /locus_tag="OO470_003650"
     CDS             174..623
                     /locus_tag="OO470_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6252758.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(624..857)
                     /locus_tag="OO470_003651"
     CDS             complement(624..857)
                     /locus_tag="OO470_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6252759.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(943..1116)
                     /gene="ortT"
                     /locus_tag="OO470_003652"
     CDS             complement(943..1116)
                     /gene="ortT"
                     /locus_tag="OO470_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6252760.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1311..1406
                     /gene="yncL"
                     /locus_tag="OO470_003653"
     CDS             1311..1406
                     /gene="yncL"
                     /locus_tag="OO470_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6252761.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1808..2617)
                     /locus_tag="OO470_003654"
     CDS             complement(1808..2617)
                     /locus_tag="OO470_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6252762.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2827..4251)
                     /gene="patD"
                     /locus_tag="OO470_003655"
     CDS             complement(2827..4251)
                     /gene="patD"
                     /locus_tag="OO470_003655"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6252763.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4273..5067)
                     /locus_tag="OO470_003656"
     CDS             complement(4273..5067)
                     /locus_tag="OO470_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6252764.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5057..5404)
                     /locus_tag="OO470_003657"
                     /pseudo
     CDS             complement(5057..5404)
                     /locus_tag="OO470_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1472 a         1442 c         1227 g         1459 t
ORIGIN      
        1 gtcatggtcg ccgggatcga atcgcaaaat caggcttcgc tgcatctgca ccagtcgctg
       61 ggatttgtcg tcaccgcaca aatgccgcag gtaggcacta aatttggtca ttggctggat
      121 ctgacattta tgcagttgca actcgacgag cgcactgaac cggacgcgat tggatgaatc
      181 agtcgctcac ccttgctttt ttgattgccg ccggaattgg tctggtagtc cagaacacgc
      241 tgatggcgcg catcacccag acctcctcta ccattctcat cgccatgttg ctgaactcac
      301 tggtggggat tgtgctcttt gtttctattt tatggtttaa acaaggcatg gcggggtttg
      361 gcgaactggt gtccagcgtg cgctggtgga cacttatacc tggcttgctg ggttcgtttt
      421 ttgtcttcgc cagtatcagc ggatatcaga atgtgggagc ggcaacaact attgcggtac
      481 tggtcgccag ccagctgatt ggcggtctgg tgctggatat cttccgcagt catggcgtac
      541 cactacgggc gttgttcggc ccaatatgtg gcgcaatttt acttgtggtt ggcgcctggt
      601 tggtggccag acgctcattt taatcagagg atggtgccgc ctttggttaa ctgttcgtgg
      661 cgcgcttcca tatcttcttt atgcttgcga ccgtgatgag caatagcggt gcgcaaacgc
      721 tgctgctggg tgtagcgatc ttcccgactt aactccgcgt catcgctcag ggcgattaat
      781 aattcgttca tatgggcaat gacgctctct tcaatcgcgg catccacgcg cgcgatgact
      841 tcatccagat gcgacatact ttctccttag ccacttgtga aatttatcga ttaatccaaa
      901 tgatataagt ctgcgtggtg ccgtcaagcc agtgagtaag atttaaaaca gtgaaaacaa
      961 cagcgccacc gggaaactca tcggccatgt ggcccccacc agaaaggcgc ttaagaaacg
     1021 gatgcgtttc ttatcgtgag aaagaaacca ggtgataaga aatgcgattg ctgccataac
     1081 cgcataaaaa acaagcatat gttgatagag agacatgtac gggcgtccag ccatatgaat
     1141 aaaacgcgcg caaatatgcg ccattttttg acgtgcatca agttttttca ccagtttgag
     1201 aaaaaatcat caaacagcaa cttgccctta tccatcttca gaatgagtat tattgcggat
     1261 tttcttaacc cgtactatac acataggggc aatgataaaa ggtggcaaaa atgaatgttt
     1321 ccagtagaac tgtagtactg ataaatgtct ttgctgctgt tggtttgttt actcttatct
     1381 ctatgagatt tggctggttt atttgatgta gtgattttgc acgcagcacg tactgtcaga
     1441 gaaatcaact ctctgacagt acgcaagatc ttctcatact ctccaagttt gcaacattat
     1501 ccttaatatt tacttctttt tgctggcatc tacattgaaa tgagtagaaa acatccccct
     1561 actgtcgtct atacaaacag cttactgatt caaacggaat cttgttttca catttttcta
     1621 tcacgaatac tttatacgcg cgggagtcta taaatgcttt atactttcaa ggaataacgt
     1681 aaatataaaa agatggcgta aagtatatgc atggcaggat gtttgcaacc tccttgatga
     1741 cgctgcccca ttgaaattgc aaaccccgcc ctggttatct tgcccattta tcatgcaaaa
     1801 cagataatta taggcctctg aatacctcag aaagattgaa ttttttatag tccctctctt
     1861 gaatatttcc atttgatatc aatcgattca tctcttcaac aaaataattt atatcatcac
     1921 acttattatt atccggaaga tctgagtagt tataattcca ccattttatt ctcaacagtt
     1981 catcaataac atttgaatca aacctaaatc tgattatttt tgcaggaact cctgcaacta
     2041 tggcatatgg gggcacatct ttggtaacaa cggaattggc agcaataaca gcaccatcac
     2101 ctattgcgat gccacctttc aatacaacat catttcctat ccagacatca tttcctataa
     2161 tcggattttc aggtttggta atgaatggtt tcaatgtcca gctacgttca aattcactca
     2221 tagcaaactt atcaaattca ccgttataag tcatcatgtg agtagtaaaa cgattcaaag
     2281 gatgatttcc acccattatt tttacatttg atgcaatact acaaaatcga cctatacgta
     2341 tattccctga aaaatggcag gtcggaaatg aaaaagcgcc gacactggag aaatttttca
     2401 caggcattgt tgaatattct tcagcaataa cattatcttc aattgttatc ttgtcacctt
     2461 ttgagaaaag aaataaattc ctttctttat acgacttctt ccccttatga tttaaaaaga
     2521 ttttattatc catacaaaac tcaaagtgtt ttttcgtcca tctgaaggta agggttttat
     2581 ttttatcttc cgtgaatttg tacaattgaa ctttcattgt taatacttct acttataaag
     2641 tattagttta ataacgatac tctaaagcct tatgctaatc aactgtgaca ggatgaaaaa
     2701 tcgccatcaa acatttagcc taatttttac gccagttatt aatgtaatca ttctaatact
     2761 taaccgccat taaactcaga acctaaaacg atagtggccg tcctataaca gccactaaca
     2821 tctaacttaa tgtttaacca tgacgtggcg gacgacggtg taatcctcca gcccataaag
     2881 tgacatatcc ttgccgtaac cagaaagttt ctgcccaccg tgcggcattt cacttaccag
     2941 catgaaatgg gtattgaccc aggtacaacc atattgcagc cgtgcgctga cgcgatgcgc
     3001 cctgcccaca tctttcgtcc ataccgaaga tgcaagtccg tactggctgt cattcgccca
     3061 gttcaccacc tgttcttcgt tgtcgaaggg cgtaacactc actactggac caaatacctc
     3121 tttttgcacg atggcatcgt cctgtaatgc gccagccagc agcgtcggcg catagtaata
     3181 gccattaccc ttgcgctttt caccgccagt gatcactttg atgtgccctg tcgctttcgc
     3241 ctcttctact gccttgctga cgcgttcgag atgcgccagc gagcttaaag gtccaagctc
     3301 cgtagactcg tcatccggcg caccagattt taacgttgcc accgcagcac ccagtttttc
     3361 caccagcgta tcgtaaatgc ctttttgcgc gtagatccga caagccgcag tacaatcctg
     3421 tccagcattg taatagccaa atgtacgtac accttcgacc actgcttcaa tatccgcatc
     3481 atcaaaaaca atcactggcg ctttgccacc aagttccata tgagtacgct taatggacga
     3541 cgcggtatgg ctgatgatgt gctcgccggt ggcgatagag cccgtcagcg acaccatccg
     3601 cactttggga tgaccggtca gcggatcacc caccgttttg cctctgccaa acagtatgtt
     3661 aatcacgcct gccgggaaga tatctttcgc cagctctgcc aacttcaacg cggtcagcgg
     3721 ggtaatttct gatggtttaa gcactacgca gttccctgcc gccagcgccg gagcaagttt
     3781 ccacgcggcc atcatcagcg gataattcca cggtgcgata gaagccacga cccccaacgg
     3841 atcgcgacgg atcatcgaag tatgaccttc aagatattca cctgccgcca gaccattcag
     3901 acagcgcgcc gcacccgcga aaaagcgaaa aacatcgaca atcgccggga tttcatcatt
     3961 gaacgcacta tgcagcggtt tgccacaatt acgggattcc agttcggcaa aaacctgacc
     4021 attttcttcg ataacatccg ccagtttcag caggcattcc gcacgcgctt tcggtgtggt
     4081 ttgcccccat tcagcaaatg ctgcatctgc cgcacgcaca gcagcatcga cctgctctgc
     4141 ggatacctcg gcaatctcca gtaaaacgtc ccccgttgcc ggattataga caggctgttt
     4201 ttccccttcg ccgctaaccc gttctccgtt aatcagtaac ttatgttgca tagcttattc
     4261 ctgtatcagt ggttattgac cattgtcgcc ttcgcgggtt agccaccagg cccccaggat
     4321 cggcaaggtt gttaccaaca taaccagcag tgccaccacg ttggttaccg gtacatcacg
     4381 cggtcgccca agctgattga gcaaccacaa cggtaacgtt cgttcatgac ctgccgtaaa
     4441 ggtcgtaacg atgatttcat cgaacgacaa ggcaaacgcc agcattcctc ctgccagtaa
     4501 cgccgaactg agattcggca acactacgta gcggaaggtt tgccagccat tggccccaag
     4561 atccattgac gcctcaacca gactccacga ggtgcggcga aaacgggcga tgacattgtt
     4621 aaacaccacc actacgcaaa aagtcgcatg accgaccacg atggtgaaaa atcccggctc
     4681 cagattgatg gttttaaagg cggttaatag cgccagacca gtgacaatgc ccggcagcgc
     4741 aatgggcagc agcaataaca gcgaaatggc gtttttgccg aaaaagtctc gtctccacag
     4801 cgcggcagct gccagcgtcc ctaacaccag cgcaattaat gtcgccagcg ccgccacttt
     4861 aagtgacagc gtcacggcat caagaatatc actacgctgt gctgccacgc taaaccagcg
     4921 cagcgtcagg ccctgcggtg gaaaactaaa cgccgcatct tcagtgttaa acgcataggc
     4981 ggcgattatc aggatgggaa aatgtaggaa aacaacgccg ccccaggccg ccagtttgag
     5041 gaaaaacggt gcgcgttcag agtgcatcga acgctcccag acgtttcacg aacgctaggt
     5101 acagtgcgat gagaataatc ggcaccaggg tgaatgccgc cgccatcggc atattgccaa
     5161 tcgccccctg ctgggaataa accatattgc cgataaaata tcctggagga ccaaccagct
     5221 gcgggacgat aaaatcgccc agtgtgagtg agaaggtaaa gatagagcca gcggcaatac
     5281 ccgggattgc cagcggcagc accacatagc gaaaggtttg tcgtggacgt gcgccgagat
     5341 cagccgacgc ctgcaacaat gacggcggca aacgctcaag cgccgcctga acgggcagga
     5401 tcatcgagta ggcagcctga cggctgcggc ttgtcatggt ctgcaattac cgttataaaa
     5461 acaggcaatg tcattgtctt tcaggtggtt atatggcccg ttcagctaaa ccccgtaaac
     5521 gtaaacccgc ctctcaaagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg
     5581 ccggatggta taccgccggg
//
LOCUS       DAJTUN010000177         5533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTUN010000177 DAJTUN010000000
VERSION     DAJTUN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.179"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            188..574
                     /locus_tag="OO470_003658"
     CDS             188..574
                     /locus_tag="OO470_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR6252765.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            582..1268
                     /locus_tag="OO470_003659"
     CDS             582..1268
                     /locus_tag="OO470_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6252766.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1246..1869)
                     /gene="tetR(B)"
                     /locus_tag="OO470_003660"
     CDS             complement(1246..1869)
                     /gene="tetR(B)"
                     /locus_tag="OO470_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6252767.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1951..3156
                     /gene="tet(B)"
                     /locus_tag="OO470_003661"
     CDS             1951..3156
                     /gene="tet(B)"
                     /locus_tag="OO470_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6252768.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3269..3862)
                     /gene="tetC"
                     /locus_tag="OO470_003662"
     CDS             complement(3269..3862)
                     /gene="tetC"
                     /locus_tag="OO470_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6252769.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3995..4366
                     /locus_tag="OO470_003663"
     CDS             3995..4366
                     /locus_tag="OO470_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6252770.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4376..>5533)
                     /locus_tag="OO470_003664"
     CDS             complement(4376..>5533)
                     /locus_tag="OO470_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR6252771.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1528 a         1022 c         1209 g         1774 t
ORIGIN      
        1 agtctagcta cagaaggaaa aatgttatcg ctatcttggt gggaaaacga atacgcagtt
       61 ctgcaatgga aaaatcatgt tttacatgcg aaagctcaac aagaagggcg agagtcaata
      121 tttgattttt acaaaattag tattgctcat attactcgcg aatattcatt taaaaaggac
      181 aaggataatg tttgatgttc acgttgtttt agataatcaa ataggacaat tagcattact
      241 aggaaaaaca ttaggtaata aaggtattgg attggaaggg ggagggatat ttacggttgg
      301 tgatgaatgc catgctcatt ttcttgttga acaaggaaag gaagctaaaa tagcgctaga
      361 gcaagctgga ctgttagtac ttgcgatccg gacaccatta attcgtaagt taaaacagga
      421 aaaaccgggg gaacttggcg aaatagcacg agtattggcg gagaataaca ttaatatttt
      481 agtgcaatac agtgaccatg ctaaccaact gatattaata acggacaatg atagtatggc
      541 tgcatctgtt acgctccctt gggcaataaa gtgaacttgc gatggctaat ttaatacgaa
      601 aagaggttac ctttgagtcc tcaatagccg cgataggggc ggctatgtct gacatttcac
      661 gagttaaaat actcagtgct ttgatggatg ggcgagcttg gacggccact gagctaagtt
      721 ctgtggcgaa tatatcagct tcaacggcga gcagtcattt atctaaatta ttagattgcc
      781 agctaatcac agtagtagct caaggcaagc atcgttattt tcggctagca ggaaaagata
      841 ttgctgaatt gatggaaagt atgatgggga tctccttaaa ccatggcgta catgccaaag
      901 tttccacgcc agtgcattta cgaaaagcac gtacttgcta tgatcattta gctggcgaag
      961 ttgccgttaa gatctatgat tccctttgtc aacagcaatg gatcactgaa aatggttcaa
     1021 tgatcacatt aagtggtatt caatattttc atgaaatggg aattgacgtt ccttccaaac
     1081 attcacgtaa aatctgttgt gcgtgtttag attggagtga acgccgtttc catttaggtg
     1141 ggtacgttgg agccgcatta ttttcgcttt atgaatctaa agggtggtta actcgacatc
     1201 ttggttaccg tgaagttacc atcacggaaa aaggttatgc tgcttttaag acccactttc
     1261 acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg
     1321 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc
     1381 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca
     1441 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc
     1501 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg
     1561 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt
     1621 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg
     1681 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg
     1741 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc
     1801 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttgc ttttatctaa
     1861 tctagacatc attaattcct aatttttgtt gacactctat cattgataga gttattttac
     1921 cactccctat cagtgataga gaaaagtgaa atgaatagtt cgacaaagat cgcattggta
     1981 attacgttac tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta
     2041 cgtgaattta ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat
     2101 gcgttaatgc aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg
     2161 cgcccagtgc tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt
     2221 tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact
     2281 ggggctgtcg cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg
     2341 ttcggttggt taggggcaag ttttgggctt ggtttaatag cggggcctat tattggtggt
     2401 tttgcaggag agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc
     2461 gctttccttg tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat
     2521 accgaagtag gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg
     2581 cccattttgt tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg
     2641 tgggtgctat ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta
     2701 gcgggtcttg gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact
     2761 aaatggggcg aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc
     2821 tttttagcgt ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt
     2881 ggtgggatcg ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag
     2941 caaggtgctt tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca
     3001 ttactgtttg ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt
     3061 attggtttag cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct
     3121 caagctcagg ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta
     3181 ttccgcgaaa tataatgacc ctcttgataa cccaagaggg cattttttac gataaagaag
     3241 atttagcttc aaataaaacc tatctatttt atttatcttt caagctcaat aaaaagccgc
     3301 ggtaaatagc aataaattgg ccttttttat cggcaagctc ttttaggttt ttcgcatgta
     3361 ttgcgatatg cataaaccag ccattgagta agtttttaag cacatcatca tcataagctt
     3421 taagttggtt ctcttggatc aatttgctga caatggcgtt taccttacca gtaatgtatt
     3481 caaggctaat tttttcaagt tcattccaac caatgatagg catcacttct tggataggga
     3541 taaggttttt attattatca ataatataat caagataatg ttcaaatata ctttctaagg
     3601 cagaccaacc atttgttaaa tcagtttttg ttgtgatgta ggcatcaatc ataattaatt
     3661 gctgcttata acaggcactg agtaattgtt ttttattttt aaagtgatga taaaaggcac
     3721 ctttggtcac caacgctttt cccgagatct catctattga aacagcttga tagccttttt
     3781 caacaaacaa tattcgtgct gagttaacca gtgattgata ggtactctta aaattttctt
     3841 gttgatgatt tttattttcc atgatagatt taaaataaca taccgtcagt atgtttatgg
     3901 tatcatgatg atgtggtcgt gacaatctta agaacattta ggttatttta tgtatattga
     3961 acagcattct cgctatcaaa ataaagctaa taacatccaa ttaagatatg atgataagca
     4021 gtttcataca acggttatca aagatgttct attatggatt gaacataatt tagatcagtc
     4081 tttactgctt gatgatgtgg cgaataaagc gggttatacc aagtggtatt ttcagcggct
     4141 gttcaaaaaa gtaacagggg tcacactggc tagctatatt cgtgctcgtc gtttgacgaa
     4201 agcggctgtt gagttgaggt tgacgaaaaa aactatcctt gagatcgcat taaaatatca
     4261 atttgattcc caacaatctt ttacacgtcg atttaagtac atttttaagg ttacaccaag
     4321 ttattatcgg cgtaataaat tatgggaatt ggaggcaatg cactgagaga tcccctcata
     4381 atttccccaa agcgtaacca tgtgtgaata aattttgagc tagtagggtt gcagccacga
     4441 gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg cctaagcgaa
     4501 ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg tcccaacctt
     4561 gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg gcgattagca
     4621 gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct agtccgtagg
     4681 caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa tagatattaa
     4741 caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaacc catggctcct
     4801 ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt gtcgagcgct
     4861 gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag attggattgc
     4921 ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata tcatgtaagt
     4981 tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt cctcttactc
     5041 gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact ttaaagccag
     5101 catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg tcggctagaa
     5161 attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc tcataaagag
     5221 taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagtcgt ttttgctcac
     5281 gaatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag ataaagctag
     5341 catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct aacaatcggt
     5401 cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg ccaagttcgg
     5461 taagagtgag agttttacag tcaagtaatg cgtggcaagc caacgttaag ctgttgagtc
     5521 gttttaagtg taa
//
LOCUS       DAJTUN010000178         5502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTUN010000178 DAJTUN010000000
VERSION     DAJTUN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.180"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(3..1217)
                     /gene="rspA"
                     /locus_tag="OO470_003665"
     CDS             complement(3..1217)
                     /gene="rspA"
                     /locus_tag="OO470_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6252772.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1423..1749)
                     /locus_tag="OO470_003666"
     CDS             complement(1423..1749)
                     /locus_tag="OO470_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6252773.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1884..2225
                     /locus_tag="OO470_003667"
     CDS             1884..2225
                     /locus_tag="OO470_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6252774.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2291..2820
                     /gene="speG"
                     /locus_tag="OO470_003668"
                     /pseudo
     CDS             2291..2820
                     /gene="speG"
                     /locus_tag="OO470_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2823..3533)
                     /locus_tag="OO470_003669"
     CDS             complement(2823..3533)
                     /locus_tag="OO470_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6252775.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3641..3946
                     /locus_tag="OO470_003670"
     CDS             3641..3946
                     /locus_tag="OO470_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6252776.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4145..5502
                     /locus_tag="OO470_003671"
                     /pseudo
     CDS             4145..5502
                     /locus_tag="OO470_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1491 a         1392 c         1274 g         1345 t
ORIGIN      
        1 ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata agcaggttca
       61 tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg tttgtctccc
      121 ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag catttgttcg
      181 gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa gtgcagcgcc
      241 gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg tacctgatac
      301 agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg ggtcagcgtg
      361 gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca gatgctgttg
      421 aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg gaagcattct
      481 tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc aatgctttta
      541 ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag caaatgttca
      601 ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc gaggtatttc
      661 tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc ataagccaga
      721 cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca ctgcacgcgg
      781 atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc atcaatactg
      841 tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc gagtaactgg
      901 taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc aaccgctgaa
      961 atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa aaactgccag
     1021 atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg atcctgcaaa
     1081 taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc cgtaataccg
     1141 tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca
     1201 gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat gaaatctacg
     1261 caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga tcacaaaaaa
     1321 aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc ttttgtcagc
     1381 tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc gcccccagcc
     1441 cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt cataaagact
     1501 tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat aaacgccacc
     1561 ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc agacaaacag
     1621 cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa
     1681 ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata gtaacgttgt
     1741 tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca taatatcatc
     1801 aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga atcaccttta
     1861 atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc ggcctgctcg
     1921 cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa actaacaaac
     1981 tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg gaaaaagagc
     2041 aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa aaagagtggg
     2101 ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg
     2161 cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc gttattaccc
     2221 cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc ccacagttaa
     2281 gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca ataacgccag
     2341 tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct ctgatctgta
     2401 tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg tgacggcgaa
     2461 aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc agaatttcag
     2521 ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc caaattagca
     2581 atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt cgataaagag
     2641 aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg tgaattgatg
     2701 catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat attccagcat
     2761 cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac cgcacaatag
     2821 cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc gggttttact
     2881 ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc atattcacag
     2941 ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt gtaatctaat
     3001 tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc gttacttttc
     3061 gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg catttgtttg
     3121 ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc ggcagaaggt
     3181 aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt ctcggcatca
     3241 cggtaataat tggcgtccag taccagagcg accacggtat tattttccag atccagtaat
     3301 tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt cgtcacttca
     3361 ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa
     3421 tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac ctctgtgcgg
     3481 tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt cacaacagtc
     3541 accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta cacgttaaat
     3601 gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct caacctgctg
     3661 tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg gatcttgtga
     3721 gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg aaagcagctt
     3781 cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg tcgtcgggca
     3841 ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta acgtctctgc
     3901 tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca ttatcgactg
     3961 aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac cttattactc
     4021 atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga ctccctctat
     4081 tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga acagggagtg
     4141 agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg ttaaatcaac
     4201 cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca ccctgcgcag
     4261 tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct gttccgtcaa
     4321 ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga cctgggtgga
     4381 aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct gtttgcgcgg
     4441 tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa tgaaacgcgt
     4501 gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc tggatactat
     4561 cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat atattcagta
     4621 ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct cggcggtcaa
     4681 acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca gcaccgcgca
     4741 aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca ccacggatat
     4801 tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc gcatgagcgg
     4861 tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca aaatgattgt
     4921 tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc tccctattcg
     4981 cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta acgaaaatct
     5041 cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa ccttacccgc
     5101 agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg acgataaaac
     5161 agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc gtatcatcaa
     5221 actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct gggggccgca
     5281 acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta ttttgacggg
     5341 caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata ccattacgat
     5401 agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct tcagctggac
     5461 ggatgctatc gatcatggcc cgcaaatgac ggcactccgc ga
//
LOCUS       DAJTUN010000179         5276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTUN010000179 DAJTUN010000000
VERSION     DAJTUN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.181"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(309..953)
                     /gene="cheZ"
                     /locus_tag="OO470_003672"
     CDS             complement(309..953)
                     /gene="cheZ"
                     /locus_tag="OO470_003672"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6252777.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(964..1353)
                     /gene="cheY"
                     /locus_tag="OO470_003673"
     CDS             complement(964..1353)
                     /gene="cheY"
                     /locus_tag="OO470_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6252778.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1368..2417)
                     /gene="cheB"
                     /locus_tag="OO470_003674"
     CDS             complement(1368..2417)
                     /gene="cheB"
                     /locus_tag="OO470_003674"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6252779.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2420..3280)
                     /gene="cheR"
                     /locus_tag="OO470_003675"
     CDS             complement(2420..3280)
                     /gene="cheR"
                     /locus_tag="OO470_003675"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6252780.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3299..4900)
                     /gene="tap"
                     /locus_tag="OO470_003676"
     CDS             complement(3299..4900)
                     /gene="tap"
                     /locus_tag="OO470_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6252781.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4946..>5276)
                     /locus_tag="OO470_003677"
     CDS             complement(4946..>5276)
                     /locus_tag="OO470_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein I (serine
                     chemoreceptor protein)"
                     /protein_id="HCR6252782.1"
                     /translation="NAVTRVTDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASL
                     VQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQ
                     DPNWETF"
BASE COUNT         1191 a         1541 c         1252 g         1292 t
ORIGIN      
        1 ttttacgcac tatttttact aagcaggtaa agagtttagg gccggaactc tgtcatttct
       61 tgcaccatga actgacgaaa aaacgttatg aagtatggca tcactacgat gtactgcact
      121 accagtcatc gtcattttct cgttcacaat ttaatgcatt gataaattta ctggcgcaac
      181 aatcacgctt cgctgatcta aaattatcaa actacaaata accatcctcc acgtactccc
      241 tgaacgatac ccgccaccct gctatcggct tggcatcatc gcgcaggccg aagttcaggc
      301 aatacaaatc aaaatccaag actatccaac aaatcgtcca cctgatcctg actggctacc
      361 acgccggctt tgctggtatc gacctgaggt ccattaagca aactctggtt ttcacgtttt
      421 ggacgcgact cctgttccgg gatgttttcc aacagcacca tcagcaactg gcgttcgatc
      481 tcctgaatga catccatcat ccgcttaata acctgcccgg tgagatcctg aaaatcctgc
      541 gccatcatga tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc
      601 agaaattgtc gtgtatctgt taccagttca cgggcgtcgg caaggtcaat cggatcggca
      661 aaccagtcat cccaacgttg ggttaacgct tttgctgatt tctccatttg atcctgatgc
      721 ggttgtgacg cctcaacact gttcagcgcc cgctccgcag cctgggcggt catctgcaca
      781 acatagtaca aacgatcgcg cgcatcgggg atggcttccg ccgcttcggc aatggcctga
      841 tccagcccca gttcccgcaa actgtcgcgc agcatatgcg tcaggctgcc gatgcgcgca
      901 atgatatcgc cagctgaatg ctcgtcagca ggtttgattg atggttgcat catagtcgca
      961 tcctcacatg cccagtttct caaagatttt gttgagtttt tcctccagcg tcgcggcggt
     1021 aaatggcttc accacatagc cactggcccc cgcttgcgcc gcagcaatga tgttctcttt
     1081 cttcgcttct gcagtcacca ttaacactgg caatgccgac atcgcgccat ccgcacgaat
     1141 tgttttcagc aattccaggc catccatatt gggcatgttc cagtcggaga taacaaatcc
     1201 ataaccgcct gcctgcaact tattgagagc gtcgacacca tcttccgctt cctcaacatt
     1261 attgaatccc agctctttca gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc
     1321 cacaaccaaa aatttaagtt ctttatccgc catttcacac tcctgattta aatacgtatc
     1381 gcctgtccgg cactaattgt tgccaacatt tgctggctta cctggctaag atcgaccact
     1441 tcgcagacac cacccatatt gatggcctcg cgcggcatgc cgaacaccac gcaacttgct
     1501 tcgttttgcg caagggtcca tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg
     1561 tcgttgccca taccggtcag gatcacccca accgcattac gccccgcctg tttggcgaca
     1621 gaatggaaca acacatctac cgaaggccga tgacggttaa ccgccgggcc gtcgtgaatt
     1681 ttgatttgat aatttgcgcc actacgcgcc agttccatat gccgatcgcc cggcgcaata
     1741 taggcatgcc ccggcaggac acgttctccg tcttcggctt ctttaacccc gatctggcaa
     1801 agcttattaa gtctgtcggc aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt
     1861 aacaatgccg ggctggaaag cggcaacggt tgcagtacgt gacgaattgc ctcagttcca
     1921 cccgttgaag caccaatcgc aatcagtttt tcagaactca acaacggccc tgccttcagc
     1981 gttgtcggtg ctgacaatgg cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc
     2041 ttttcagcaa tcatttcgct atacgccagc attccttcgc gaatacccag ttgcggtttg
     2101 gtgacaaaat ctattgcccc cagctccagc gcgcgcagcg tgacttctga tcctttgccg
     2161 gtcaaggaag aaaccatcac aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa
     2221 tccagtccgt ccatccgcgg catttcaaca tccagcgtca gcacatcggg attgaatttc
     2281 ttaatcaagt cacgcgcaac cagcggatca ggcgcggtcg ccaccatttc catgtcgcta
     2341 tggctgttga tgatttctgt catgatctgg cgcatcagtg ccgaatcatc gacagataac
     2401 accctgattt tgctcatcgt taatccttac ttagcgcata caccgtctga ccacgcagcg
     2461 tgaagcggcg ctcgaggtgg ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg
     2521 gtttaaggag cggaacaaag cggcgcaaaa tctcctgctg ggtagtttga tcgaagtaga
     2581 tcatgacgtt acgacagaag atcgcatcaa acggccccgg cacggtgtac tgtttcgcca
     2641 gtaaattcag cggggcaaaa tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc
     2701 cttcatgcgg tcccgtccct cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt
     2761 tcaactcttc atggcgatag ataccgcttc tggctttttc cagcacttcg gtgtcgatat
     2821 cactggcaaa cactttccag cgtccgggca cggtgcccaa tgtgtcagcc agagtcatcg
     2881 caatgctgta cggctcttcg ccggtcgaag ccgccgcgct ccatacgcga tcctcgccag
     2941 aacggcgacg tgcgtgatcc gcgagcagag ggaaatgatg agcctcacgg aaaaatgccg
     3001 tcagattcgt ggtcagcgaa ttgataaacg cctgccactc accgctgtgc tgattagatt
     3061 ccagcaagtt cagataatga ccgaaatccg tcagtcccag cgaacgcaaa cgacgaacca
     3121 gtcggttgta aaccatgtcg cgtttatggt cagccagaac gatcccggct cgttgataga
     3181 tcaattgact tatccgccga aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta
     3241 acaataaaga cgtttgccca cagggcagag atgaagtcat agcgccttct caatcacttc
     3301 aggataccac tggcgcaatt tgtaactgcg ccgactcatg tcgtgctact tcatgttctt
     3361 caagggtaaa taccgccaca cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg
     3421 ttgccaccgc cgcttcttct accagcgagg agttctgctg agtcacctga tccatctggc
     3481 tgacagcctg tgcaacctgc tcaatccccc gccgttgttc ttccgacgcc gaagcaattt
     3541 ctcccataat gtcgttcacg cgggtcaccg aactgacaat atcggtcatg gtcgcggcgg
     3601 cgttattcac cagtttcgaa ccctgctgga cacgattgac tgactcttcg atcagccctt
     3661 tgatctcttt tgctgcctgc gcgctacggc tggcaagatt gcgcacttca cctgccacta
     3721 ccgcaaaacc acgcccctgc tctccggcgc gagccgcttc cactgccgca ttcagggcca
     3781 gaatattggt ctggaaagca attccgtcga taacgctgat aatgtcgcca attttttgcg
     3841 agctggtggc gatctcctgc atggtgtgag tcatggtact gacctgaaca ccgcccgcct
     3901 gcgccgttgt cgcggcattt tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt
     3961 gccctaccgt ggcggttaat tgctccatac tggcggccgt ttgtgccagc gatgccgcct
     4021 gctgttcggt acgacttgag agatcgttat tgcctgcgac aatctccgcg atcccaatgt
     4081 gcatctcatg gcttccctta cgcacatcac tcaccgtccc acgcaaagcc tgctgcatgg
     4141 tcttcaggct ggcaaaaatg gcggtgatct cattacgacc atataccgca atcggacgcg
     4201 ccagattacc cgcagcaatg ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa
     4261 tcatcttgcg cgtccaccac agcgcactgc tgatgtagat cgccgcaaca ataatcatgc
     4321 tgataaacac cagcgccgaa atctgatagt tacgctggct ttgcgcactg gcggcttcca
     4381 gcacatggtt gatctccagc tgccaggcct caaagttaac gtcaaacgca ttctgtgatc
     4441 cctgcaccgg cgcagtgagg aaatccgaaa gttgattact ttcaagccag gtcgcctgat
     4501 gttcgagatc gttgtgccag cgggcaaaac ttttctccgt ctctttttgc aatgccctga
     4561 cgtgctcgtt gcccgcagtc atcgccataa aacttttaaa cagcgtggtg gattgcgtca
     4621 gactggcgcg cgccgtcgtc atcaacgttt taatgtcatc cgccggatag ctaagcgctg
     4681 tcagagtgcc tgctttgttc agcgcggtac tggcctgtaa cattaccgcc cgagtttgcg
     4741 ctaatgccgc acgttgctga ttgctctgct cgccgtgatt cagtcgttgc aaatcgtcgc
     4801 gaaatgccca aaaagacatg ccgttactgc caatctgcaa gatcccgcag agaatcaaaa
     4861 ttaaaaacag cgtggtcgaa attcgaatac gattaaacat caacgctccc catcaggcgg
     4921 caatgaccgc gttagtaaaa actcgtcaaa atgtttccca gtttggatct tgttcagtaa
     4981 ttcgcagtcg tggctgtgcc ggtggttgct cactggcagg acgggatggt gtttgcggtt
     5041 tattggtgag tgggctggct gccagacgga acgcggaaac cgcttgcgtt aaacgactcg
     5101 cctgttcttc cagcgcagct gcggcggcag ctgattcctg caccagcgat gcgttctgtt
     5161 gcgtgacgcg atccatttcc gaaaccgcca atgcgacttg atcgatgcca cggctctgtt
     5221 catccgatgc cgatgcaatc tcgcccataa tgtcagtcac gcgagtgaca gcattg
//
LOCUS       DAJTUN010000180         5179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTUN010000180 DAJTUN010000000
VERSION     DAJTUN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.182"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1241)
                     /gene="pcnB"
                     /locus_tag="OO470_003678"
     CDS             complement(<1..1241)
                     /gene="pcnB"
                     /locus_tag="OO470_003678"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6252783.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALR"
     gene            complement(1280..2206)
                     /gene="gluQRS"
                     /locus_tag="OO470_003679"
     CDS             complement(1280..2206)
                     /gene="gluQRS"
                     /locus_tag="OO470_003679"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6252784.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2243..2698)
                     /gene="dksA"
                     /locus_tag="OO470_003680"
     CDS             complement(2243..2698)
                     /gene="dksA"
                     /locus_tag="OO470_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6252785.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(2876..3580)
                     /gene="sfsA"
                     /locus_tag="OO470_003681"
     CDS             complement(2876..3580)
                     /gene="sfsA"
                     /locus_tag="OO470_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6252786.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(3595..4125)
                     /gene="thpR"
                     /locus_tag="OO470_003682"
     CDS             complement(3595..4125)
                     /gene="thpR"
                     /locus_tag="OO470_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6252787.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4199..>5179
                     /gene="hrpB"
                     /locus_tag="OO470_003683"
     CDS             4199..>5179
                     /gene="hrpB"
                     /locus_tag="OO470_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6252788.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRL"
BASE COUNT         1158 a         1357 c         1429 g         1235 t
ORIGIN      
        1 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
       61 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      121 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      181 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
      241 agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta
      301 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
      361 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
      421 cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
      481 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
      541 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
      601 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
      661 agatccttca taccaccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
      721 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
      781 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
      841 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
      901 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
      961 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1021 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1081 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac cgtcacctgt
     1141 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1201 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     1261 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     1321 actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt tgctcgacgc
     1381 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     1441 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     1501 cttgtggatt aagcgccagc ggcagatgaa tgtaatcagg cactttccag ccaaaaagct
     1561 ggtacagcgg gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     1621 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     1681 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     1741 gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     1801 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     1861 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     1921 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     1981 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2041 cttctatgcg taccagccag cgaccttgcc gggcgcgagc ctgcaaatag ctgccgagcg
     2101 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2161 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     2221 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     2281 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     2341 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     2401 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     2461 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     2521 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     2581 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     2641 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     2701 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     2761 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     2821 gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     2881 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     2941 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3001 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aaaataaccg
     3061 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3121 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3181 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttcggagc
     3241 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     3301 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc
     3361 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     3421 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     3481 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     3541 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     3601 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     3661 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg ggaatgtcac
     3721 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     3781 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     3841 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     3901 cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     3961 ctctgcgctc acttcgccta aaaatgccag agtcaggtgc aaattatcgg cggcgaccgg
     4021 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcggatttc
     4081 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4141 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4201 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     4261 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     4321 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     4381 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     4441 ctaccggatg cgtgcgcaaa actgcgtcgg gctgaatacc cgcctggaag tggttaccga
     4501 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     4561 ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc tgttactcga
     4621 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     4681 tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag ggcgctcgtt
     4741 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgttgc
     4801 ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat ttttacctgg
     4861 cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca gtgatgtggt
     4921 gctctgcccg ctgtatggcg tgttgtcact gaacgatcag cgaaaagcga tcctcccggc
     4981 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5041 cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgct ttgatccgcg
     5101 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     5161 cgggcgcgcc gggcgtctg
//
LOCUS       DAJTUN010000181         5023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTUN010000181 DAJTUN010000000
VERSION     DAJTUN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.183"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..175
                     /locus_tag="OO470_003684"
     CDS             <1..175
                     /locus_tag="OO470_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="regulates the expression of uxuBA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6252789.1"
                     /translation="LIRKDPHAAKLAMWQHLENTKIMLFNETSDDFEFNADRYLFAEN
                     PVVHLDTATSGSK"
     gene            520..1182
                     /locus_tag="OO470_003685"
     CDS             520..1182
                     /locus_tag="OO470_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6252790.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1186..1569
                     /gene="mzrA"
                     /locus_tag="OO470_003686"
     CDS             1186..1569
                     /gene="mzrA"
                     /locus_tag="OO470_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6252791.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            1716..2070
                     /locus_tag="OO470_003687"
                     /pseudo
     CDS             1716..2070
                     /locus_tag="OO470_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2108..2413
                     /locus_tag="OO470_003688"
     CDS             2108..2413
                     /locus_tag="OO470_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6252792.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            2416..2820
                     /locus_tag="OO470_003689"
     CDS             2416..2820
                     /locus_tag="OO470_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6252793.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            2810..3109
                     /locus_tag="OO470_003690"
     CDS             2810..3109
                     /locus_tag="OO470_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6252794.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3295..3591
                     /locus_tag="OO470_003691"
     CDS             3295..3591
                     /locus_tag="OO470_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6252795.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            3661..4647
                     /gene="yqjG"
                     /locus_tag="OO470_003692"
     CDS             3661..4647
                     /gene="yqjG"
                     /locus_tag="OO470_003692"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6252796.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            4940..>5023
                     /locus_tag="OO470_003693"
     CDS             4940..>5023
                     /locus_tag="OO470_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6252797.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFIL"
BASE COUNT         1261 a         1226 c         1264 g         1272 t
ORIGIN      
        1 gttaattcgc aaagatcctc atgctgctaa gctggcaatg tggcagcatc tggaaaacac
       61 caaaatcatg ttatttaacg aaaccagcga cgatttcgag ttcaatgccg accgctatct
      121 gttcgccgaa aacccggtag tgcatctcga tacggccacc agcggcagca aatgaaattc
      181 ctgtccgaca ggcgctgcaa tgcgcctgtt tgtctcgtcg gtaagcgaaa agtataaagt
      241 gtcagcctgt gtaaatcctc tcgccaccct cccctgcaat cagcaaaatc agactccacg
      301 gacatgtaat tttgataacg aacaacgttg acctttgtta caattagatt caatttgaat
      361 ttatgctttt gaatgctttc ttatctcacg ttttaacagg gaatagttca ggctgtgttg
      421 atgtatcaaa cccgcagaac ataccaaaac agcaataaca ttgcggtagt gcatctttta
      481 aaaccagcgt ggcgttaacc gattcaccag gaataatgaa tggaactttt gacccaattg
      541 ctgcaagccc tgtgggcgca ggattttgaa accctggcca atccatcgat gattggcatg
      601 ttgtattttg tcttgtttgt aattttgttc cttgaaaatg gcttgcttcc ggcggccttt
      661 ttaccgggcg acagtttact ggtattggtc ggcgtgttga ttgcgaaagg cgcgatgggt
      721 tacccgcaaa cgattctgct gctgaccgtt gccgccagcc tcggttgctg ggtcagctat
      781 attcaggggc gatggctggg caatacccgc accgtacaaa actggctatc tcatttaccc
      841 gcgcattatc atcaacgcgc acaccatctt tttcataaac acggtttatc ggcgctgtta
      901 attggtcgct ttattgcatt tgtcagaaca ctgctgccga cgattgccgg gttatcaggg
      961 ctgaataacg cgcgctttca gttcttcaac tggatgagcg gtctgctgtg ggtattgatc
     1021 ctgacaactc tgggttacat gctcggcaaa acgccggtat ttttaaagta cgaggaccag
     1081 ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg tgtttggcct ggcaggttct
     1141 ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt aagggatgca aatacctcgc
     1201 atgtcgcttc gccagctagc ctggtccggc actgttttac ttctggtcgg cacgctgttg
     1261 ctggcctggt ccgcggttcg ccagcaagag tctacgctgg cgattcgtgc cgttcatcaa
     1321 ggcacaacga tgccagacgg tttttcgatc tggcatcacc ttgacgctca tggcattcct
     1381 ttcaaaagta tcacccccaa aaacgacact ctgttaatta catttgactc cagcgaccag
     1441 agcgccgccg caaaagcggt cctcgacaga acattgcccc atggctacat cattgcgcag
     1501 caggacaata acagtcaggc tatgcagtgg ctgacccggt tacgggataa ctctcatcgc
     1561 ttcggataac ttcctgttat ccgaaacata tcactcactt tggtgatttc accgtaactg
     1621 tctatgatta atgaggcggt ggttaacact accgcttcgt tgtctggaca atcgttcctt
     1681 tgtaataggt ccgaatcaca atggaaggtt caagaatgaa ataccgcatc gctttagctg
     1741 tttctctctt tgctcttagt gccggtagtt atgccactac cctgtgtcag gaaaaggagc
     1801 aaaatatcct taaggagatc agctatgccg aaaaacacca aaaccagaat cgtattgacg
     1861 gtctgaataa agccctgagt gaagtccggg ccaactgttc agatagccag ctgcgtgccg
     1921 atcatcacaa gaaaatcgca aagcagaaag atgaggtggc ggaacgccag caagatttag
     1981 ccgaggcgaa gcaaaaaggc gatgccgata gaacggaaac tggcagaagc gcaggaagag
     2041 ctgaaaaagc tggaagcgcg cgactactaa ctcacaatag tcactactta ctcacctgga
     2101 gaaaactatg tcgaaagaac acactacgga acatctgcgt gctgagttga aatccctttc
     2161 cgatacgctg gaagaggtgc ttagctcatc tggcgagaag tcgaaagaag agttgagtaa
     2221 gattcgtagc aaagcggagc aggcactgaa acagagccgt tatcgcctgg gtgaaaccgg
     2281 tgatgccatc gccaaacaaa cccgtgtcgc ggcggcgcgt gctgatgagt atgtgcgtga
     2341 aaatccgtgg accggcgtgg gaattggcgc tgcaatcggt gtagtgctcg gcgttctgct
     2401 gtcgcgtcgt taattatggc ggacactcat cacgcgcaag ggcccggtaa aagcgttctg
     2461 ggcatcgggc agcgaattgt ttccatcatg gttgaaatgg tggaaacacg tctgcggctg
     2521 gcggtggtgg agctggaaga ggaaaaagct aatctctttc aacttttact gatgctgggc
     2581 ctgacgatgc ttttcgctgc atttggtctt atgagcctga tggtgctaat tatttgggcg
     2641 gttgatccgc aatatcgcct gaatgcgatg attgccacca ccgtggtgtt gctgctactg
     2701 gcactgattg gcggtatctg gacgttacgt aaatcgcgta aatccacctt gctgcgccat
     2761 acacgccatg agctggcaaa cgatcggcaa ctgctcgagg aggagtcccg tgagcagtaa
     2821 agtcgaacgt gaacgacgta aggcgcaact gcttagccag atccagcaac aacggctgga
     2881 tctttccgcc agtcgtcgtg aatggctgga ggcaacaggc gcttacgatc gtcgctggaa
     2941 tatgctgcta agtctgcgct cctgggcgct ggttggcagt agcgtgatgg cgatctggac
     3001 gattcgccat cctaatatgc tggtccgctg ggccagacgc ggttttggcg tatggagcgc
     3061 ctggcgtctg gttaaaacga ccctcaagca gcaacagctt cgcggttaac ttccctctgg
     3121 ccggagcaat tccggcctta tccctcaaat tttttgaata tttttgacag ttttccttgc
     3181 taacaatcat cattcaccac gtttatgatt ctctccatcg acagcaacga cgctaatagc
     3241 gcgcccttgc acaaaaaaac aatcagcagc ctgagtggcg cagtggagag tatgatgaaa
     3301 aaattagaag atgttggtgt actggtagcg cacattttaa tgccgattct gtttattacc
     3361 gcaggctggg gaaaaatcac cgcttactct ggtacccaac aatatatgga agcaatgggt
     3421 gtcccgggtt ttatgctgct gacggcattt ttatttcaca gcaactttgc tgaaggcgtc
     3481 aactcgctga tgttcatgaa aaacctgaca atttctggcg gattcctgct gctggcaatt
     3541 accggtccgg gcgcgtatag catcgaccgc ctgctgaata aaaagtggta agcacgcgct
     3601 atacttaacg ataaaaaacg aggaggaagc tcctcgtttt tgctattgga ggagagaaaa
     3661 atgggtcaac tgattgacgg cgtctggcat gacacctggt acgataccaa atctaccggc
     3721 ggtaaatttc aacgttcagc ttccgcattt cgtaactggc tcactgccga tggcgcacct
     3781 ggccccactg gcacaggcgg ttttatcgca gagaaagatc gttatcatct ctatgtttca
     3841 ctcgcctgcc cgtgggcgca ccgcacgctg atcatgcgca aactcaaagg actggaaccg
     3901 tttatttccg tttccgtagt gaacccgctg atgctggaaa acggctggac ctttgatgac
     3961 agttttccgg gagcaaccgg agatacgctc tatcaacatg aatttctgta tcagctttat
     4021 ctccacgccg atccacacta cagcggacga gttactgttc ccgtgctgtg ggacaaaaag
     4081 aaccacacca tcgtcagcaa cgaatcagca gaaatcatcc gcatgttcaa taccgcgttt
     4141 gatgcgctgg gcgcaaaagc gggtgattac tacccaccag ccctgcaaac gaaaattgac
     4201 gaacttaacg gctggattta tgacaccgtt aacaacggcg tgtataaagc tggttttgcc
     4261 accagccagc aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct ggcgcgactg
     4321 gagcagattt taggccagca tcgttacctg accggcaacc agctaaccga agccgatatt
     4381 cgcctgtgga ctacgctggt gcgttttgat ccagtgtatg tgacccactt caagtgtgat
     4441 aagcaccgca tcagcgatta cctgaatctg tatggcttcc tgcgcgatat ctaccagatg
     4501 ccggggatcg ccgaaactgt caatttcgat cacatccgta atcattactt ccgcagccat
     4561 aagaccatca accctacggg gattatttca attggtccgt ggcaggatct cgatgaaccg
     4621 catggacgag atgttcgctt cggttaaaaa aaggcacctt acgaggtgcc tctgataatt
     4681 agctaagtgc atgttgttca tgtcggatgc ggcgtgaacg ccttatcaaa cctacaaagc
     4741 gtttatattc aatatgttac aatgaccgcg taggccagat aagcgtagcg catctggcga
     4801 ttttgcgctt gccatcagtc ttagcgcgcc ttcacggcac tttattaatt ccttgaacga
     4861 atatttactg ccatttcatt cacgtctatt cttaatttgc tgctttaaaa acaagtgatt
     4921 gagcaaaatt gaggcaaaaa tggactggta tctgaaagta ctaaaaaatt atgtcggttt
     4981 ccggggtcgt gcgcggcgca aagagtactg gatgtttatt ctg
//
LOCUS       DAJTUN010000182         5006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTUN010000182 DAJTUN010000000
VERSION     DAJTUN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.184"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="OO470_003694"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="OO470_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6252798.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="OO470_003695"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="OO470_003695"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6252799.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="OO470_003696"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="OO470_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6252800.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="OO470_003697"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="OO470_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6252801.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5006
                     /gene="yidZ"
                     /locus_tag="OO470_003698"
     CDS             4377..>5006
                     /gene="yidZ"
                     /locus_tag="OO470_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6252802.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICW"
BASE COUNT         1139 a         1204 c         1250 g         1413 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgg
//
LOCUS       DAJTUN010000183         4983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTUN010000183 DAJTUN010000000
VERSION     DAJTUN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.185"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(6..1355)
                     /gene="ghxP"
                     /locus_tag="OO470_003699"
                     /gene_synonym="yjcD"
     CDS             complement(6..1355)
                     /gene="ghxP"
                     /locus_tag="OO470_003699"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6252803.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMVGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(2061..2525)
                     /gene="soxR"
                     /locus_tag="OO470_003700"
     CDS             complement(2061..2525)
                     /gene="soxR"
                     /locus_tag="OO470_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6252804.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2611..2934
                     /gene="soxS"
                     /locus_tag="OO470_003701"
     CDS             2611..2934
                     /gene="soxS"
                     /locus_tag="OO470_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6252805.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(2937..4514)
                     /gene="pdeC"
                     /locus_tag="OO470_003702"
                     /pseudo
     CDS             complement(2937..4514)
                     /gene="pdeC"
                     /locus_tag="OO470_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
BASE COUNT         1376 a         1293 c         1141 g         1173 t
ORIGIN      
        1 aaagattaga tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc
       61 gtaccgatgt tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg
      121 aagccgatca tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc
      181 agacccacca tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc
      241 agcaggccaa cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc
      301 ggagagagga acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt
      361 ttaccgcccg ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc
      421 accaggccag agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg
      481 atctgcccat ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg
      541 gcatcaaata ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc
      601 gcgcccataa tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc
      661 atggcgaaaa cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata
      721 ccgataatgg tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc
      781 gccagaccta ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc
      841 agaccatcaa gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg
      901 aacagaccga taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc
      961 cagctacgga tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca
     1021 cccagcgcga caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc
     1081 gtcagggaga tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata
     1141 gaaccgagtc cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct
     1201 ttacccaaca tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac
     1261 ccggcaacca cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg
     1321 agtgaaccgc cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa
     1381 ttttcgtcaa gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca
     1441 ggtttgcggc aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac
     1501 gggtggtaaa aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt
     1561 tgctgctttt ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac
     1621 gttatcgtcg atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat
     1681 aaaaaatcgt ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga
     1741 agaaacgtcg gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct
     1801 cgaatgaggt gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa
     1861 gacaaagacc ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga
     1921 taattccaaa agagctcgat cagtcccctc gccccttcgg ggagagggtt agggttaggg
     1981 gaacaggcag caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt
     2041 cagcttgtcc ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct
     2101 tcttctccta agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca
     2161 caaccaatac atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc
     2221 aactcttctc gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct
     2281 tctggcaaca cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg
     2341 attttgataa ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg
     2401 ttacggatac tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca
     2461 ccgctgcgtt tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt
     2521 tccataaatc gctttacctc aagttaactt gaggaattat actccccaac agatgaatta
     2581 acgaactgaa cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt
     2641 atcgcatgga ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa
     2701 tcaggctatt caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt
     2761 ggcgattaca ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag
     2821 cgtccgattt ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc
     2881 gttttccgtc ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat
     2941 tgcccgcgcg ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc
     3001 ggcatcgcct tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag
     3061 ttaacctgct cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc
     3121 agctcgatga tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca
     3181 aacgatttat cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag
     3241 tagccaatac caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt
     3301 aatcggcgtc attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg
     3361 ctgcggacgc accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt
     3421 atgaaaatcg gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta
     3481 cgcgcccaga tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc
     3541 ctccttttct gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg
     3601 ccaacgtaac aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg
     3661 gtaataaaga caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa
     3721 atagttttgt cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc
     3781 gggcaacgca aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc
     3841 aacaatggct gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct
     3901 ttgtacggtt aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt
     3961 gcttaacgag aaaaaggttt tcgtcaccgt atcatacaca ccccattgca atgtcgggtc
     4021 atcagacatc acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg
     4081 catataggtc attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac
     4141 gttaggttca cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag
     4201 cgatgagcat aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata
     4261 ttcaacattc gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca
     4321 tcgctcacta aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca
     4381 aaggttcgga gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa
     4441 agaatgatgg gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt
     4501 gcacgatgac tcattgctat caatttcctg cctgtttata cccagccatt gcaaggtgaa
     4561 accctggcta ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt
     4621 tgaaattgat acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt
     4681 aattttctac aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc
     4741 acataatttg tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg
     4801 atatagttca caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc
     4861 cgcgatagcc agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg
     4921 ctcttttcag aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg
     4981 gca
//
LOCUS       DAJTUN010000184         4856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTUN010000184 DAJTUN010000000
VERSION     DAJTUN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.187"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..969
                     /locus_tag="OO470_003703"
     CDS             <1..969
                     /locus_tag="OO470_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6252806.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1550..2773
                     /locus_tag="OO470_003704"
     CDS             1550..2773
                     /locus_tag="OO470_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252807.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTISSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3020..3586
                     /gene="slp"
                     /locus_tag="OO470_003705"
     CDS             3020..3586
                     /gene="slp"
                     /locus_tag="OO470_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6252808.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3742..4272
                     /locus_tag="OO470_003706"
     CDS             3742..4272
                     /locus_tag="OO470_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6252809.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4314..4856)
                     /locus_tag="OO470_003707"
                     /pseudo
     CDS             complement(4314..4856)
                     /locus_tag="OO470_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1556 a          997 c          900 g         1403 t
ORIGIN      
        1 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
       61 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
      121 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
      181 tgctcgacaa tacccatgtt acgtggattg ttgtcagcga aagccgggtt tggtccgact
      241 ctcacttttt tatttgtttc accattactt aatcccatta tcgtcggact aatgtggatg
      301 acctttggtt ggaaagtcac cttgttgtac gcgattatcg cagccggcgt ctccgttctt
      361 gccagtatta tcctggattc tctggggttt gaacgtcata tcattgccag taaaagctca
      421 tcagcaaatt gttgtgctcc agcaaaaact tcgccgggaa cgacatatac gcccataaaa
      481 gtgagttgtt gtagtccagc ggctaaagcc attgagaacc ccgtagttac ctgttgcagt
      541 accaaagctg tagtaagtat taatcccata aaactagcca ccaaagatgc gttgcaacag
      601 tttaaagatg tactgccata tcttttgtta ggagtattaa taggatcttt tatttatggc
      661 tttattccct cagcgtggat tgccgctcat gcaggagcag ataatccctt cgccatccca
      721 ttaagcgccg ttgtcggtat tccgctgtat atccgggctg aggcggttat ccctctggca
      781 tctgttctga tgacaaaagg aatgggtctg ggagcattaa tggcattaat catcggcagt
      841 gccggcgcaa gcctgacgga agtgatatta cttaaatcaa tgttcagaat gccgatgata
      901 gctgcattcc tgacggttat attaggtatg gctatcttga tgggctattt gactcaattg
      961 ctattttaaa tagatgccag cagtaaacta taagatgcat agttagtgcc atatccttct
     1021 aagtagaaaa tcatagacag gataatcctt aaaaatcaca aatgatttaa ctaatgcatt
     1081 gagaatgatg acggctcggt caacaagtca tcaacagaca ataaccacgt aatcatgctt
     1141 ctctgacatt ttatcggaga agcaatgact ctttgtctca gtaccctcct cctttttaaa
     1201 aggcagattt cagtcgctaa tacaacacat ttttaaatac actttaacct ggttcattat
     1261 tacacatcta aaacatcaca aaaatattac ttcacacact atcggagaac ataataactt
     1321 tttaaaatga gcgttttttg atagttgtta acgttttcct ttacgatact atcactgccc
     1381 tttttttata cacaaatatt accgggtgga aatgaaaggg aatttaaagg taattatatc
     1441 aggaagatat aacgcattat atttattgtg tgtagaagaa aatccattcc tgttctaatt
     1501 atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta tgtcaattga
     1561 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
     1621 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
     1681 acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg atttagcgca
     1741 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
     1801 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
     1861 accacttacg caacagacac aatcaatact taatcagatt cataacatta gatacgaaaa
     1921 ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca acaatccaat
     1981 cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc aaaccatttc
     2041 ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata taagagacat
     2101 tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa atgaaaatag
     2161 gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc aagatatgtc
     2221 caagctagtc ctaaccttat caaaatttta ttttgaagat ctacttaata taaatttcac
     2281 tgacgcaaat ttatttaaca ctattttttc acacaaggaa aatcccatcc ccaaactaca
     2341 taaatatgaa caacatcttg acaaacaaat caatggtttg ttttctacat tgttaactat
     2401 taatgacaac tctttacgcg cgaaagcgga aattgcttcg agaataattg atttcttaga
     2461 ggcaaaggta gtgaatcttt catttgatga catattaaaa tataagcaag agttcaaaaa
     2521 aatatgttat aaacaacttc aagagtttac aacaccctct ctatacaata aaattcaaaa
     2581 atgggcaaca atgtctaaaa atgaattcat agcatttcat tacgagacac ttcaacccga
     2641 aaagatttca tacccctttt atttaaagag agatctgcct aatgaaaaag atataaatta
     2701 tggggtggaa atagaaatac catctggcaa aagaattcgc ttatcaaatc attatcagaa
     2761 tataatacca taatattgat tattagcact ttataatcat tgaataaaaa tagattttat
     2821 gtaattttaa aacaaagggt tatattattg ggcgatggat actcatacca cgccccaaaa
     2881 taacggaatt gaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag
     2941 gcatatcacc tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt
     3001 tgttgataag gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt
     3061 tttgcttgcc gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat
     3121 tcaaaaaagt tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg
     3181 ctttggtggg aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc
     3241 tgtattaccg ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact
     3301 gctcgccaga caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat
     3361 cctcggtacc attcagggtg aacaaccagg ctttatcaat aaagtcccgt ataacttcct
     3421 ggaagtgaat atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta
     3481 taacctgtgg gattacggct atggtgcatt ctggccggaa ccgggctggg gtgcgcctta
     3541 ctacaccaat gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgaaagat
     3601 gcagatgctt tctcagcatc tgcatcatgt attacataaa attaatatac agtaagctaa
     3661 ctattattat tataagccct gtcctgttaa ttacctttgg caaactgatt ataaagttaa
     3721 tgtccgcacc aggagtcggt tatgtttctt ataattacca gggatacgat gttcttcacc
     3781 gcgatgaaaa acattctgag taaaggtaat gtcgttcata tacagaacga agaagagatc
     3841 gacgtaatgt tgcatcagaa tgccttcgtc attattgata cattaatgaa taatgtattt
     3901 cattctaatt ttctcactca aattgaacga ttaaaacctg tccatgtcat tgttttctcc
     3961 ccctttaata ttaaacgctg tctggggaaa gtaccggtga cctttgttcc gcggactatc
     4021 actatcattg attttgtcgc actcatcaat ggcagttact gctctgtgcc tgaagcgaat
     4081 gtgtcacttt cgcgcaagca acatcaggtt ctgagctgca ttgcgaatca aatgacaact
     4141 gaagatattc tggagaaact gaaaatatcg ctaaaaacgt tctactgcca taaacacaat
     4201 atcatgatga tcctcaatct taagcggatc aatgagctgg tacgccatca gcatattgat
     4261 tatctggtgt gaatttcagg cttacggtga gtctggctac gctgccacac agattagcta
     4321 attgaaacgc ctttcacccc tgccatacct tttaataatc gcaacaggtc ttctatcaac
     4381 gttgtggcat gaagctggat atcaatggcg accacttcat gatcttcatt ttcctgcaac
     4441 gaaactaaat cagtttttat tttttgctgc ttaaaccagt cgagcattga taccgtatta
     4501 ccgttcacta atgtcagctg taaatggtaa tttttgttca tcaggcggaa ggttaattga
     4561 tggaagactt ccagcaccaa caaggtcatc actgaaccat aaatacccag ttcgtacata
     4621 ccgctgccaa taaccatacc tatgcggcgg tcacccagat atccgctgcc gtcgtcagac
     4681 ctacaatatt ttggttacga acgagaatgt tacctgcacc gataaacccg acgcccgtca
     4741 ccacctgagc agcaatacgg ctggggtcga gtccgacgtg atctaaagac agcacgtcag
     4801 caaaaccata tttcgaaaca atcataaaca gggcgcttcc catgccaatt aataca
//
LOCUS       DAJTUN010000185         4827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTUN010000185 DAJTUN010000000
VERSION     DAJTUN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.188"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(605..1021)
                     /locus_tag="OO470_003708"
     CDS             complement(605..1021)
                     /locus_tag="OO470_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252810.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1048..1188
                     /locus_tag="OO470_003709"
                     /pseudo
     CDS             1048..1188
                     /locus_tag="OO470_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1188..2228
                     /locus_tag="OO470_003710"
     CDS             1188..2228
                     /locus_tag="OO470_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6252811.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2256..2345)
                     /locus_tag="OO470_003711"
     tRNA            complement(2256..2345)
                     /locus_tag="OO470_003711"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2309..2311),aa:Ser,seq:cga)
     gene            2439..3236
                     /gene="mtfA"
                     /locus_tag="OO470_003712"
     CDS             2439..3236
                     /gene="mtfA"
                     /locus_tag="OO470_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6252812.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3337..3412
                     /locus_tag="OO470_003713"
     tRNA            3337..3412
                     /locus_tag="OO470_003713"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3370..3372,aa:Asn,seq:gtt)
     gene            3574..4827
                     /locus_tag="OO470_003714"
                     /pseudo
     CDS             3574..4827
                     /locus_tag="OO470_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1333 a         1127 c         1093 g         1274 t
ORIGIN      
        1 tgttcagcat ggattgagcc tcagattaat gttaagcgtc ttgcaggacg cgtaatgtta
       61 actggggctt ttctctatct gccttttggt gttcatgcct gagacagata gcctcaagca
      121 cccgcagtca ttctacttaa ctaagatttc cctgcaaacc gtttttgtcc ggcacagtaa
      181 atatccaact aaaccaatag cgttcgctgt atttaccgcc agtattcaat gcacatgacc
      241 gccatgaaca cccctaaaaa aagggcattt atatgtccaa acattaatat caaaacatca
      301 attttttcca tataccttgc tgtgaagatg atgggcatac atgatgcgaa caaccagaac
      361 gcaacaaaca aaaactgcaa tgcgtttttc attattcccc ctacaatcaa tgtgcaataa
      421 catttaaaca cacctcaatt tggccggaca tataaatatc taaaccagaa aaaatcactt
      481 acatagcgtt acaaactctt tagtctaaat actcatcgta aaacattccc catacttatc
      541 agtccgttcc gcgccaggta gcttattgcc ttatctggca acctataatc aggtttccgc
      601 tttttcagtt ggctggtcgt ttaaccgaca tagttaaccc attaatctgg ttgccggatg
      661 ctggtggatt ttcgcgtttt aattgttcat aaaagtgcac agctttaacc agttcttctg
      721 atgtaaccgg gactggtggg gcagtgaata aggcctgaat ttcatagttc ggcctgtcgt
      781 tacaatcctc ttttttcggt acatatttcc agtcaccaac ccacaactcc ccctgagagt
      841 ccataacacc ttttttcacg tagcgatatc gccacgctat cggctctgct tccagcgatg
      901 cccgtgcaat tttgaataac tcgccctcta ctcgcgccat ccctgaattg gggtggcatt
      961 tcgtaatcgc tatttttaat ttggcttctt cgattaattg ttcttttgtt aattcagtca
     1021 ttttcattac cgccctggta atgctgccac cagcgaaaat ggttaatgga aaacgggctt
     1081 ccgttctata ccaatcgtag tggcaaaccg attgtcagcc gggatctatt tacctgcaat
     1141 aaaactttac caccacgcga ggtagagccg aattttggtg cgatctgatg ggaagacgaa
     1201 ggaaaaatcc tgaacacgaa aaattacctc caaatgtata cccaaataaa tatagttatg
     1261 tatggaaacc aacatccaga gaatctgtaa cactaaccgc catcaaggat ggtttagctg
     1321 ctttatggaa aaagtatgag gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc
     1381 gcttgtggga aaaattcctc gccagcgcct attacagtga cctcagtcca agaacacaaa
     1441 aagattatct gcaacatcaa aaaaagttgc ttgccgtatt cggtaaggtg ccggcagatt
     1501 ccataaaacc agaacacatc cgtcgataca tggacaagag aggggaacag agtaaaacgc
     1561 aagccaacca tgaaaaaagc agtatgtccc gcgtttacag ttgggggtat gagcgagggt
     1621 acgtgaaggc taacccatgt gcaggtgtaa gtaaattcaa ggccaaaaac cgcgaacgat
     1681 atgtaaccga caaagaatac caggcagtat taagcgttgc acctcttcct gtttttatcg
     1741 caatggaaat tgcctatctg tgtgcagcga gggtttccga tgtgttatcg ctgaaatggg
     1801 agcagattgg aaacgacggg atctttatcc agcaagggaa aacaggaaaa aaacagataa
     1861 aagcatggag tccacgatta caggcggcga tcgaaaaagc aaaacagtta ccaacatccg
     1921 cctatgtaat cagcaatcaa tacggcaacc gatatatgta caaaggcttt aacgaaatgt
     1981 gggtagaagc aagaaatcgc gcaggcaaaa tttcaggtat tttaaccgac ttcacctttc
     2041 atgatctgaa ggcgaaagga atttcagact atgaaggaag cagtcgggat aagcaacttt
     2101 tctctggtca caaaaccgag gggcaagtgc taatctatga caggaaggtt aaagtttcac
     2161 cgacacttga tgtcccgtta cctgaaaata ttccaagaaa atattccaag tgtgattttt
     2221 gtcactgact taatgatgta taagtgattg aattttggcg gagagagggg gatttgaacc
     2281 cccggtggag ttgcccccac tccggttttc gagaccggtc cgttcagccg ctccggcatc
     2341 tctccgttca gatggttgtc atgatgccag gaaatttggc attttaacag tccctgtccg
     2401 tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat gattaagtgg ccctggaaag
     2461 tacaagaatc agcacatcaa actgcccttc cctggcagga agcactatcg atcccccttt
     2521 taacgtgtct gacagaacag gaacaaagca aattggtcgc tcttgccgaa cgttttttac
     2581 agcaaaaacg gcttgttcct ttacagggct ttgagctgaa ttcattaaga agctgccgga
     2641 tagcacttct attttgcctg cccgttctgg agttaggact ggaatggctg gatggttttc
     2701 atgaagtctt aatttatcct gcgccatttg tggtcgatga tgaatgggaa gacgatatcg
     2761 gtctggtgca taaccaacgt attgttcagt caggtcagag ctggcagcaa gggcctatcg
     2821 ttttgaactg gttggatata caagattctt ttgatgcttc tggttttaac ctgattattc
     2881 atgaagtcgc tcataagctg gacacccgta acggcgatcg cgccagcgga gttcccttta
     2941 tttcgttgcg tgaggttgct ggctgggaac acgatcttca tgctgcaatg aacaacattc
     3001 aggaagaaat cgaattagtt ggtgagaatg cggcgagcat tgatgcttat gttgccagtg
     3061 atcctgctga atgttttgcc gtactttctg aatatttctt tagcgcccca gaactttttg
     3121 ctcctcgttt cccttcattg tggcaacgtt tctgtcaatt ttatcaacaa gatcctttgc
     3181 agagactgca tcacgctaat gatacagact cgttttcagc gacgaatgtt cattaattaa
     3241 caactttgaa gattaattaa ccaattgaaa tggcttatga aatttagtgt tgacagacaa
     3301 ggtaccgcta agtaatatgc gccccgttct cacgattcct ctgtagttca gtcggtagaa
     3361 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caattttctg
     3421 ttttcatgca tccttgcgaa tctttatctg atgttgattc aacaggttag tgtgaaaact
     3481 cttctcggtg cattttgatt ttaccctacg catcgggaaa aattggtggt caaatctggg
     3541 atcaggttag ttcgataatg gagtgacccc catatatccc ttaccgacgc aaaaatccgc
     3601 accctcaagc cttctgataa accctttaaa gtctccgatt ctcacggtct gtatctgctg
     3661 gtcaagccgg gtggctcccg ccactggtat ctcaaatacc gtattagcgg taaagaatcc
     3721 cgcattgcgc tgggtgccta tccagccatc tccctgtctg atgcgcgaca gcaacgtgaa
     3781 ggtatccgta aaatgctggc gctgaatatc aacccggtac agcagcgggc tgctgaacgt
     3841 ggctcacgaa caccggagaa agtttttaaa aacatggcgc tggcgtgaca taaaagtaac
     3901 aggaaatggt cgcagaacac cgccgaccgt ctgcttgcca gcctgaacaa tcacatcttt
     3961 ccggtcatcg ggaacctacc tgtatcagaa cttaaacccc gtcatttcat tgacctgctg
     4021 aaagggatcg aggaaaaagg tctgctggag gttgcgtccc gcacacggca gcacctgagt
     4081 aacataatgc gccatgcggt ccatcaggag ttaatcgata cgaaccctgc agcaaacctt
     4141 ggcggcgtga ccacacctcc tgtcagacgg cactatcctg ccctgccgct ggagcggctg
     4201 cctgaactgc ttgaacgtat tggggcatat catcagggcc gtgaactgac ccggcatgcc
     4261 gttctgctga tgctgcatgt gttcattcgc tccagtgaac tgcgtttcgc ccgctggtca
     4321 gagattgatt tcacaaaccg agtctggacg atacccgcga cgcgagaacc cattattggc
     4381 gtgcgttatt ccggccgcgg ggcaaaaatg cgaatgccgc atatcgtccc cctctcagaa
     4441 cagtccatcg ccattctgaa acagattaag gatatcaccg gtaataatga actgatcttc
     4501 cccggcgacc ataacccgta taagccaatg tgtgaaaaca cggtcaataa ggcactgcgg
     4561 gtgatgggtt acgacacgaa aaaggatatc tgcggtcacg gcttccgggc aatggcatgc
     4621 agtgcgctga tggaatcggg tttatgggca aaggacgcag tagaacgcca gatgagtcat
     4681 caggagcgca ataccgtgcg catggcttat attcataagg cagagcacct ggaagcccgc
     4741 aaagcgatga tgcagtggtg gtcggattat ctggaagcat gccgagaatc ttatgcaccg
     4801 ccttatacaa ttggtaaaaa taagttt
//
LOCUS       DAJTUN010000186         4766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTUN010000186 DAJTUN010000000
VERSION     DAJTUN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.189"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(112..270)
                     /locus_tag="OO470_003715"
     CDS             complement(112..270)
                     /locus_tag="OO470_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6252813.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            complement(254..565)
                     /locus_tag="OO470_003716"
     CDS             complement(254..565)
                     /locus_tag="OO470_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6252814.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(750..1640)
                     /gene="cyoE"
                     /locus_tag="OO470_003717"
     CDS             complement(750..1640)
                     /gene="cyoE"
                     /locus_tag="OO470_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6252815.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(1652..1981)
                     /locus_tag="OO470_003718"
     CDS             complement(1652..1981)
                     /locus_tag="OO470_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6252816.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(1981..2595)
                     /locus_tag="OO470_003719"
     CDS             complement(1981..2595)
                     /locus_tag="OO470_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6252817.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(2585..4576)
                     /gene="cyoB"
                     /locus_tag="OO470_003720"
                     /pseudo
     CDS             complement(2585..4576)
                     /gene="cyoB"
                     /locus_tag="OO470_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            complement(4598..>4766)
                     /locus_tag="OO470_003721"
     CDS             complement(4598..>4766)
                     /locus_tag="OO470_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6252818.1"
                     /translation="NQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGME
                     GMDMSHAESAH"
BASE COUNT         1286 a         1271 c         1178 g         1031 t
ORIGIN      
        1 tgaaggacgc cagaaacgcg ttgtctttcc agcgaatatg ccgtaaaagt acagatgcca
       61 atcagttcac cactttgtct gtcaataagt gctttagccg cacagtcgct attacgcaca
      121 cttttcttta gcgatgcgcg tagcgcgtac aaatttatcg ataacagggt taccgcaatc
      181 aaactgtttt tgcccaggat aagtaatatc agactgataa ttacaggaga ggactatggg
      241 cagagtaatc tcttcatgct tgtcgaccat cgctgttttt ccttttcatt aacgccttga
      301 gggcgagcgt cggttgagca ggttcagcga tgagttggtt catcagttcc cagctttgat
      361 tcgaaagctc acggcgacgt tggttatcaa gcacactttc ggcgcgttcc atagcagcgc
      421 tcaaaataaa agcgcttaaa tctaccccaa cggcttgcgc agcttcacga agtttgtttt
      481 tggcgtcggg actagttttg agttcgaccc gcgcattttt cagcgtttta tcgacatcgt
      541 atagagcggc tgtttttcca ggcattatga cttctttgat taactgaatg aaatcgtagg
      601 gagtcgatct catacataat acggctatca accgtttgat taatttatgt gcatctgctc
      661 actctgtcaa gagatacggt tattagccgt atctctgatc tcagacaata aaaaaggtgc
      721 cgtagcacct ttttaataga gaggttttgt taccacacag cagccagcag cgtatgcgag
      781 tccggtacca taaaatcaac ggacatcatc accgagaggg cagtgatggc gatgatagag
      841 aagccgaaca gcttgcgcgc ccagattctg tcatcagcaa ctttataacc gcgcagagcc
      901 atacctaacc accagacgct aaccgccgcg gcgaccacca gatatttata cccagcgtaa
      961 ccgccaagag agagcatcag cgtggcaacg gcaaaggcga tgatatacag cgtgatgtga
     1021 ttcttcgcca ccgaaatgcc ttttaccact ggcaataccg gaatgtttgc cgcctggtaa
     1081 tccttaaagc ggaaaatggc gatggcatag gagtgaggca tctgccacag gctgaagata
     1141 gccagcagga tcgctgcgcc gctatcaaac tcgccggtta ctgcacagta gccaatcacc
     1201 gacggcgcag cgccggagag cgaaccaatc aatgtgccgt agactgagtg gcgtttcatg
     1261 tacaggctat agacgccgac ataaaccaca aagcccatca cccccagcca gcaggccagc
     1321 ggattcgcgc caaaccacag cagcataaag ccagcaatac ccagcaaggt ggcgtacacc
     1381 agcgagacag caggagagat caggccttta accagcaccc gattcttcgt cctttccatc
     1441 tttctgtcga tatccctgtc gatgtagttg ttaaacacac aacccgacgt cacaaccagt
     1501 gacaccccaa ccagcgtgta gataaacagg ggataatcaa tgctgccctt tgaggccagc
     1561 aggaatcccc caatcaccga gatcaggttg ccaaagatga tgcctggttt cgttacttgc
     1621 aggtattgct taaacatcat aaccgccgct cttagtgcat catcatgttg tagttgaggt
     1681 tccacataat ccagatggag cctacaacca ggatagcgat gattagcacg gtgaagacaa
     1741 acgccgtcat gttccagcct tcatctgatt tggtattcat gtgcaggaag cacaccagat
     1801 gcaccagaac ctgtaccact gccattgcca ggattgttcc cagaattacg gccggagagg
     1861 cagcccctgt catcaccatc cagaacggaa tcaccgtcag aatgatcgac aggataaagc
     1921 ctgtcatgta ggtttttacg ctgccatggg acgcgccgct gtgatcggta gaatgactca
     1981 ttacatcgcc cccatcagat aaacaacagt gaacacacag atccaaacca catccaggaa
     2041 gtgccagaac aggctcaggc acatgatgcg ggtacggtta gtgctggtca ggccgcgacg
     2101 ggcgatttgc accatcagca ccgccatcca gataagacca gaagtgacgt gcagaccgtg
     2161 cgtgccgacc agcgcaaaga acgctgacag gaagccgctg cgatccggcc ccatgccgtt
     2221 aacaatcagg tgatggaatt catagatttc catcccgata aatccggcac caaacaacca
     2281 ggtcaacgcc agccaggaga taacctggct tttgttgttt ttgtacatgg cgatagccgc
     2341 catgccgtag gtgatggagc tgaacaacag caagaaagtt tcaaccagaa cgaacggcag
     2401 ttcgaaaatg tccttacctg tcgggccgcc tgcggtgccg ttcaccagaa cggcataggt
     2461 agcaaacaag attgagaaca gaatgcagtc gctcatcagg tagatccaaa atccgaagat
     2521 tttggttccg cctgcatcgt ggtgcccgtg ttcgtgcgcg tgggcagtcg cgtgcgtcaa
     2581 agtatcagtt gccatttttc agccctgcct tagtaatctc atcgaaatgc tggttttcca
     2641 gtttttcgat ttctgccacc ggcacgtagt aatccacgtc ctcgtcgaag cttttcacga
     2701 tccaggtgat gatcatgcct gcgaagccaa caatcgccag ccaccagata tgccagatca
     2761 tggcgaaacc gaagatggtg gagaaagctg caatgacgat acctgcaccg ctgtttttcg
     2821 gcatatgaat ttcttcatag tggtcaggct ttttatacgc ttcgcctttc tctttcattt
     2881 cccagaatgc atcacgttcg tgaacgtgcg gcactacggc aaagttatag aacggaggcg
     2941 gggaagaggt tgcccactcc agcgtacggc caccccacgg gtcgccagtc aggtcacggt
     3001 tctggtcgcg gtcgcgaata gaaacgtaca tctgaataac gaggcagaga atacccagcg
     3061 caatcagtac tgcaccgctg gctgcaatca tcagcatggt gtggaactgc gggtcaatct
     3121 gctggctcaa acgacgggtc atgcccatga agcccagcgc atacagcggc ataaaggcaa
     3181 cgaagaagcc gatgatccag aaccagaacg cgcgtttacc ccaggtttcg ttcagtttga
     3241 aaccgaacgc tttaggccac cagtaggtca tccctgcgaa gcagccgaag accacgccgc
     3301 cgatgatcac gttatggaag tgcgcaatca ggaacaggct gttatgcaga acgaagtccg
     3361 cgcccggtac tgccagcagc acgccagtca taccgcccac cgagaaggtg acgataaaac
     3421 cgatagtcca cagcatcgca gaatggaaca cgatgcggcc ctgatacatg gtgaacagcc
     3481 agttgaagat cttcaccccg gtcgggatgg cgataatcat tgtggtgata ccaaagaagg
     3541 cgtttacgtt cgcgcccgca cccatcgtaa agaagtggtg cagccaaacg atgaacgaca
     3601 gcacggtgat gcagacggtt gcccatacca gcgaggtata accaaacaga cgtttacgcg
     3661 agaaggttgc cgcaatttcg gagaacacac cgaaaacagg caggatcagg atgtaaactt
     3721 ccgggtggcc ccaggcccaa atcaggttga tgtacatcat catgttgcca cccatatcgt
     3781 tggtaaagaa atgggtgccc agatagcgat ccagggtcaa caacgcgacg gtaaccgtca
     3841 gaattgggaa ggaagcaata atcagtacgt tcgcgcacag tgatgcctag gtaaatactg
     3901 gtatcttgaa catggtcatg cccggtgcgc gcatcttcag aatggtaacg aagaagttga
     3961 taccggtaag cgtcgtacct ataccggata gctggagact ccatatccag taatcaacac
     4021 cgactcccgg actgtactct attcccgata gcggtggata ggccagccag ccggtctgcg
     4081 caaattcgcc cacgccgaga gaaacgttaa ccagaatcac gccaacaaca gtaaaccaga
     4141 agcttaagtt gttgaggaac gggaacgcaa cgtcacgcgc gccgatctgc agcggaacca
     4201 ccaggttcat cagaccgata acgaaaggca tcgccacgaa gaagatcata atcacgccgt
     4261 gcgcggtgaa gatctgatcg tagtggtgag gtggcaggaa gcccgcttca cccgccgagg
     4321 caagagcctg ctggctacgc atcataatgg cgtcagcaaa accacgcagc aacatcacaa
     4381 tcgccacgat gatatacatg ataccgaggc gtttatggtc gacggaggtc agccactctt
     4441 tccacaggta ggtccactta ccgaagtaag tgatcaggcc aacgagcgcc agacctccca
     4501 aaataatgcc agcgatcgta accatgacga tagtttcatg gaacgggact gcatcaagtg
     4561 ataattttcc gaacatcttt attcttcctc aaccccttta atgggcggat tccgcgtggc
     4621 tcatgtccat gccttccata ccttcgtgtg cactgtgctc accttctggc tgggtcatgt
     4681 ccatgctctt accgtgagcc ataaacttgt taatcacatc ggcaaacaag tctggtttca
     4741 cgttggagaa atattccacc tggttg
//
LOCUS       DAJTUN010000187         4738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTUN010000187 DAJTUN010000000
VERSION     DAJTUN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.190"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..454
                     /locus_tag="OO470_003722"
                     /pseudo
     CDS             1..454
                     /locus_tag="OO470_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="OO470_003723"
     CDS             514..621
                     /locus_tag="OO470_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6252819.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO470_003724"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO470_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6252820.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO470_003725"
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO470_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6252821.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2257..3414)
                     /gene="yehY"
                     /locus_tag="OO470_003726"
     CDS             complement(2257..3414)
                     /gene="yehY"
                     /locus_tag="OO470_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6252822.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3421..4338)
                     /gene="osmF"
                     /locus_tag="OO470_003727"
     CDS             complement(3421..4338)
                     /gene="osmF"
                     /locus_tag="OO470_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6252823.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4590..>4738)
                     /locus_tag="OO470_003728"
     CDS             complement(4590..>4738)
                     /locus_tag="OO470_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HCR6252824.1"
                     /translation="TQTVSFPIDIEALKFWNQQMKYDAEPGKFNVFIGTDSARVKKGE
                     FELL"
BASE COUNT         1161 a         1406 c         1189 g          982 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctgctg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccagtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgcctc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcatta gtgagagatc
     2941 gttaagcgtg ccggagtaca gcaaccacag cggaataatg gcaatctgca tatgcaacaa
     3001 ccagcgccac agcggatgcg tggagattcg gcggatggca tcgctacagg ccagcaatgc
     3061 cagcgccgca gccagccaga aacctctgcc gaggctggta cgcgccagcg cactgccatt
     3121 ttgcgccagt tgggtcgccg cctttccagc tccccacacc aacaatacga agacgaattg
     3181 cgccagaatg agtgcaaaaa tgctgccttt tttagcgggg acaaaacagg ctgtcagcca
     3241 ggcgcaacca acgcccacca gcatccagat cgtttgcggc cacagttgcc agagatgaag
     3301 cccttcaccc gagactaaac gattaggcgc gtaactgata aacggcagcg ccgctgcgat
     3361 tgccgtcagc aacaacagca gcgccagaac aggattaata cgtagataag tcacgggaaa
     3421 ttacttcgcc cacccttttt gtttcaggta gtcggcagcc acttttttgg catccagtcc
     3481 ttccactgca atgctggcat tcagttgctg caatgttttt gcatcgaggc tggcgaagac
     3541 tggctgtagc cactgtgcca tttgcggata ctccttcaac accgattcac gcaccactgg
     3601 tgcaggcgcg tagataggtt gcacgccttg cggatcgctt aaggtttgca gccccagcgc
     3661 cgcgaccgga ccgtcagtgc cgtaagccat tgcggcatta acgccagagg tttgctgggc
     3721 agcggcttta atcgtcaccg ccgtgtcgcc accagccagt gacagcaact gatcctgacc
     3781 gagcttaaag ccataggctt tttcaaacgt gggtaacgca tcggcgcgtt cgataaactc
     3841 tgccgaggct gccagtttga aggtgccgcc ctcttgcaga taacgactca ggtcggcaag
     3901 cgaagtgagt ttgtttttct ctgccacatc ctgacgcacg gcgatagtcc aggtgttatt
     3961 tgcaggcgcg ggcgtcagcc agattaactt gttgtgctcc gcatcgagct ttttgacttt
     4021 ctcgtagccc tgcccggcat ttttccacgc tgcatcgttt tcatctttaa agaagaaagc
     4081 gccattgccg gtatattccg gatagatatc cagttcaccg gaagtaatcg ccccgcgccc
     4141 cacaggagtc gttccgagtt gcactttatt caccgtcggt acgccgtggc tttcgagtac
     4201 ctgaaagatg atgttgccga gcagcgcgcc ttcggtatcg atttttgaac cgactttaac
     4261 gggggaagcc gcctgtagcg gcaggctcac ggctgccaac ataaccagtg aacctgccca
     4321 gagctttaag agtggcatga tgctttcctc attcttttac tgttgttttc agcgaattaa
     4381 gagaaaagca tagttgataa tgacagagtt agcctgagcg gaaggattca gttgcagaat
     4441 cagataaata cttaagaggc atattcggtc cggcgtttcc tgccggatgc ggcgcgagcg
     4501 ccttatccgg cctacaaagg gcgaaaattc agcaagttaa gatatgaaca tcaggcaaag
     4561 ggcgcaaacg tcgcgccctt actaaagcat tacagcaact caaactcgcc tttcttaacg
     4621 cgtgcggaat cagtgccgat aaatacattg aacttgccag gctcggcgtc atatttcatc
     4681 tgttgattcc agaacttcag cgcctcaata tcgatcggga agctgacagt ctgagttt
//
LOCUS       DAJTUN010000188         4655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTUN010000188 DAJTUN010000000
VERSION     DAJTUN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.191"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..387
                     /locus_tag="OO470_003729"
     CDS             <1..387
                     /locus_tag="OO470_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6252825.1"
                     /translation="TAGVYLRAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVPMLD
                     LVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVDEAI
                     IFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(441..1046)
                     /gene="azoR"
                     /locus_tag="OO470_003730"
     CDS             complement(441..1046)
                     /gene="azoR"
                     /locus_tag="OO470_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6252826.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1247..>4655
                     /gene="hrpA"
                     /locus_tag="OO470_003731"
     CDS             1247..>4655
                     /gene="hrpA"
                     /locus_tag="OO470_003731"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6252827.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAA"
BASE COUNT         1142 a         1176 c         1283 g         1054 t
ORIGIN      
        1 actgctggtg tttatttaag ggcgtttcca cgacatattc cggcacagaa tgcggttctg
       61 gacgtcacct ttgaattccc tcgtggacga gcgacaaaag atcgactcta tttctgtgta
      121 ccgatgttgg atctggtggt tccggaagag ggggagctcc gacaggccgt ggcgatgctg
      181 gaaacattac gcgaagagca aggcagcgtt ctggtccatt gcgcattggg attatcgcgc
      241 agtgcgctgg tagtggcggc atggctgtta tgttacggac actgtaaaac agttgatgaa
      301 gcgattatct ttattcgagc cagacgctcg catattgtgc ttaaggaaga gcacaaagcg
      361 atgttgaaat tatgggaaaa caggtaagtg gattgagatg tggactgaat atctacagtc
      421 cacatcaaga ccgtgtccgg ttatgcagca acaatgctgt cgatggctgc ttttgcgtca
      481 gactgtgctt tcgctgccat ttccggaccg tatgcgatcc cttcggcgaa gacaaatttc
      541 acatcggtaa tgccgataaa gccgaggaac gtggacagat acggcgtcac caggtccgtt
      601 ggtccatctt tgtggatccc accgcggctg gtaataacga tggctttttt acccgttacc
      661 agaccttccg gaccgttctc ggtatagcgg aaagtaacgc ctgcgcgtgc aaccaggtca
      721 aaatagtttt tcaactgagt tgagatgttg aagttataca tcggtgccgc aataacgata
      781 acgtcgtggg ctttcagctc ggcaatcagc tcatcggaaa gcgccagagc ttcttgctga
      841 cgcggagtca gcggcgcatc gctcggacgc agagcgccaa ccagttcgcc atccagtacc
      901 ggaatcggat ttgcagctag gtcgcgaacg gtgatttcat cagcggagtg cttttcgctc
      961 cattgttcaa caaaataatc ggacaactga ttagactgag agtaccctgc caggatgctg
     1021 gatttaagaa ctaatacctt gctcatggtg tttccttata gatgtttgaa tggacgatgc
     1081 cccgttgctt gctgacactt tattcacaat cctgccacag agatagcgca ataaatcgaa
     1141 gtctatgttc gaatttattg aacaacgcat agaaagccgc gatgtggtac tctatatcta
     1201 tcatttaaaa gaaaattaat caggcagact actgcccact aacgttatga cagaacaaca
     1261 aaaattgacc tttactgcct tgcaacagcg gctggattcg ctgatgctgc gcgacagact
     1321 gcgtttttct cgccgtctgc attgtgttaa gaaggttaaa aatcctgatg cacaacaggc
     1381 cattttccag gagatggcga aagagattga ccaggcggca gggaaagtcc tgctgcgtga
     1441 agcggcacga ccggaaatca cttatcctga caatttaccg gttagtcaga aaaaacagga
     1501 catactcgaa gcgattcgtg atcaccaggt ggtgatcgtc gccggggaaa ccggctccgg
     1561 taaaacgact cagctaccga aaatctgtat ggagctgggg cgcggtatta aagggctgat
     1621 cggtcatacc cagccgcgtc gcctggcggc acgaacggtg gccaaccgta ttgcggaaga
     1681 gctgaaaacg gagccgggcg gttgcatcgg ttacaaagtg cgtttcagcg atcacgtaag
     1741 tgataacacg atggtcaagc tgatgaccga cgggatcctg ctggcggaga tccagcaaga
     1801 ccgcctgctg atgcagtacg acaccatcat tattgacgaa gcgcacgaac gcagcctgaa
     1861 tatcgatttt ctgctcggct atttgaaaga gttgctgccg cggcgtcctg acctaaaaat
     1921 cattatcact tccgcgacta tcgacccgga acgcttttcg cgccacttta ataatgcgcc
     1981 gattattgaa gtctccggtc ggacctatcc ggtggaagtg cgctatcgcc cgattgttga
     2041 agaagccgat gacaccgagc gcgatcagtt gcaggcgatt tttgacgccg tagacgaact
     2101 aagccaggaa agccccggcg acattctgat ctttatgagc ggcgagcggg aaattcgcga
     2161 taccgccgat gcgctgaaca agctgaactt acgccatacc gaaatcttgc cgctttatgc
     2221 gcggctttcg aacagcgaac agaaccgcgt gttccagtcg cacagcggac ggcgcattgt
     2281 gctggcgacc aacgtcgcgg aaacctcgct gaccgtaccg gggattaaat acgtcatcga
     2341 ccccggtaca gcgcgtatca gccgctacag ctatcgcacc aaagtgcagc gtttgccgat
     2401 tgaacccatt tcgcaggcgt ctgccaatca gcgtaaaggc cgctgtggtc gtgtgtccga
     2461 agggatctgt attcgtctct attccgaaga cgatttcctc tcgcgcccgg agtttaccga
     2521 tccggagatt ctgcgtacca acctggcctc ggttattttg cagatgaccg cgctggggct
     2581 gggcgatatc gctgcgttcc cgtttgtcga agcaccggat aaacgcaata tccaggatgg
     2641 cgtgcgtctg ctcgaagaac tgggcgcgat caccacggat gaacaggcca gcgcctataa
     2701 actgacgccg ctcggtcgcc agctctcgca gttgcctgtc gacccacgtc tggcgcgtat
     2761 ggtgctggaa gcgcaaaaac atggctgcgt gcgtgaggcg atgattatca cgtccgcgct
     2821 ctccatccag gatccgcgcg aacgtccgat ggacaaacag caggcatcgg acgaaaaaca
     2881 tcgtcgcttc cacgacaaag aatccgactt cctcgcgttt gtaaatctgt ggaattatct
     2941 tggcgagcag caaaaggcgc tttcttccaa cgccttccgt cgcctgtgtc gtaccgatta
     3001 cctcaactat ctgcgcgtgc gcgaatggca ggatatctac acccagttgc gccaggtggt
     3061 gaaagaactt ggcattccgg tcaacagcga accggcggag tatcgtgaaa ttcacatcgc
     3121 cttactgacc ggtttgcttt cccatatcgg catgaaagat gccgataaac aggaatatac
     3181 cggtgcacgt aacgcgcgtt tctccatctt ccccggttct ggtttattca aaaagccgcc
     3241 gaaatgggta atggtggcgg aactggtaga aaccagccgc ctgtgggggc gcattgctgc
     3301 gcgtatcgac ccggaatggg tagaacccgt tgctcagcat ttgattaaac gcacctacag
     3361 cgaaccgcac tgggaacggg cgcagggcgc ggtgatggca acggaaaaag tcactgttta
     3421 tggtttgccg attgttgctg cgcgcaaggt caactacagc cagatcgatt cggcgttatg
     3481 tcgtgaactc tttattcgcc acgctctggt ggaaggtgac tggcagacgc gtcacgcatt
     3541 cttccgtgaa aacctgaaac tgcgggccga agtggaagag ctggaacaca aatcacgtcg
     3601 ccgcgatatt ctggttgatg acgaaacgct gtttgagttc tacgaccagc gcatcagcca
     3661 cgatgtaatc tccgctcgtc acttcgatag ctggtggaaa aaagtcagcc gcgaaacgcc
     3721 tgatttgctc aactttgaaa agagcatgtt gatcaaagaa ggggcggaaa aaatcagcaa
     3781 gctggattac ccgaacttct ggcatcaggg caatctcaag ctgcgtttga gctatcagtt
     3841 tgagccaggc gcggacgcgg acggtgtgac cgtacatatt ccgctgccgt tacttaacca
     3901 ggttgaggaa agcgggtttg aatggcagat ccccggtctg cgccgcgaat tggtgattgc
     3961 tctgattaaa tcgttgccga aaccggtacg ccgtaatttt gtgcctgcac caaactacgc
     4021 cgaagcgttt ttaggccgcg tcacaccgct ggagttaccg ttgctcgaca gccttgagcg
     4081 cgagttacgg cggatgaccg gcgttaccgt tgaccgcgaa gactggcact gggatcaggt
     4141 gcccgatcac ctgaaaatca ccttccgcgt ggtggatgac aaaaacaaga agctaaaaga
     4201 agggcgctcg ctacaagatc tgaaagatgc gctgaaaggc aaagtgcagg aaacgctgtc
     4261 cgcggtggcg gatgacggta tcgagcagag cggcttacat atctggagtt ttggtcagct
     4321 gccggaaagt tacgaacaga agcgtggcaa ctataaagtg aaggcgtggc cggcgctggt
     4381 ggatgaacgc gacagcgtga cgatcaaact gtttgataac ccgctggagc aaaaacaggc
     4441 aatgtggaat ggtcttcgcc gtctactgct gctaaatatt tcctcgccga tcaagtattt
     4501 acatgagaag ttaccaaaca aagccaagct ggggctgtac tttaacccgt atggcaaagt
     4561 gctggagctg atcgacgact gtatctcctg cggtgtggat caactgatcg acgccaatgg
     4621 tggcccggtc tggacggaag aaggctttgc tgcgc
//
LOCUS       DAJTUN010000189         4646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTUN010000189 DAJTUN010000000
VERSION     DAJTUN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.192"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1658)
                     /gene="mscM"
                     /locus_tag="OO470_003732"
     CDS             complement(<1..1658)
                     /gene="mscM"
                     /locus_tag="OO470_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6252828.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAV"
     gene            complement(1680..2648)
                     /gene="psd"
                     /locus_tag="OO470_003733"
     CDS             complement(1680..2648)
                     /gene="psd"
                     /locus_tag="OO470_003733"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6252829.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(2745..3797)
                     /gene="rsgA"
                     /locus_tag="OO470_003734"
     CDS             complement(2745..3797)
                     /gene="rsgA"
                     /locus_tag="OO470_003734"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6252830.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            3892..4437
                     /gene="orn"
                     /locus_tag="OO470_003735"
     CDS             3892..4437
                     /gene="orn"
                     /locus_tag="OO470_003735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6252831.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1040 a         1234 c         1263 g         1109 t
ORIGIN      
        1 accgccagcg gataaggcca cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc
       61 ggtaacggtg acgcgacgag aatcgaccag aaaagagtgc gcaacgtcag ccagaagtga
      121 tcctgagtca ctttgccgac tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg
      181 cgcgagtaaa tactgcaacc gaccagaatc aacgcgccaa acagcggcaa aattgtctct
      241 ttgctggtca gcatcatcac actggctttg cccaactgac tgaaggtgtc cagcgaaatg
      301 agacgacgca gatcctgggc gatttccagc ggccaggcga tggtcatcgg gcgcacgtca
      361 gaggcccaga acagatagcg gtgcgttgct tcgttcacct ctttcagcgc atcctccagt
      421 tgcccgttgg agactttcag cttggtcagt tccaatagta gcgtgtcgcc accctgcaac
      481 aatgagttca gcaactcacg ctgagtgcgc agttgtgctt ccagaatacg gttttgctcg
      541 gcggtcagcg gctgaccgtc ggcctgatgg atttgccgta gcagcggctg tttattaagt
      601 aaatcctcat aacgtaaccg ttgcacacgc aactgcgcca tttcggtatc aagctgttgt
      661 ggttttggca tttctggtag ccgtgccacc tgcgcccgca gcgcttcgcc gagcaggttg
      721 gacgatccca gccattgcga ctgttcacgc agcgtattca acgcctgccg gacctgtaac
      781 gtctggctgg cagcctgacg ctgttgcgag gcaacgagct ccatccgctg cgcctgttga
      841 ttcagcgctg ccgatagttc gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa
      901 tcggcactgt tttctgccag taattcggta ctttccagcg cccgctccgc ctcaagctga
      961 cgttggctat tcaactgatt acgtaaggcc tgcaaatacg catccagttg ctggctctct
     1021 ttttccgcca gctctgagcg taagcgcgct aattcctggc ggttattggc agacagctgc
     1081 gccagctcca gttcatcaac gagcgcctta agacgtgcag agtcagactg caacgcgaaa
     1141 ttttgtgcct gattgagcgg agtattgccg gtaagcgttc ccaggcggcg ctcgatctca
     1201 tttaactgac ggcgggcgtc ggtttgctgt tgcggcagtt gattcagcga atcggcaatc
     1261 tcgcgggctc gctcctgctc ttgctgggcc tgacggcttt tatccagcaa ctggctgctg
     1321 acctggagaa tttcctgatt cagcgcgtcg gtagacattc ccggcgacac gctgcgcggc
     1381 tcgtcacgaa tgttgtttaa ttgtgcgcgc agagtagcgg agagtttcgg ataattatcg
     1441 ataacttgct gatattgttt gatgcgctca agggaacctt ttcgttcctc aagcgcattt
     1501 aaggcagact ggagcgcctc tacgacttcc ggctgtgcgg gtttcgccgc ttttgcctgc
     1561 tccagttcct gagtgatttg tttgctatcg ggggccgtcg cggcgtacgc cccccaactg
     1621 aggcaccagg ccatcagaaa agtgataatc aggcgcacgt cagcgtttcc tttgatggat
     1681 tagacctggt cttttttatc gtcaaccaat gggctggcgt cgtgttctgc ttcgatctct
     1741 tcagcaggaa gtggggcggg ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc
     1801 agcggctggc caattttcgt gacagacagg ctttccagtt gctcaaccag attcacttta
     1861 cccggtgcaa acaggttgat aacggtggaa ccgagtttaa agcgacccat ttcctggcct
     1921 ttcagcagtg ccacagaacc gtcgttttcc ccggcaggcc aggtccagcg cttgatgata
     1981 ccttcgcgcg gcggcgtaat ggtgcccgcc cagaccgtct caatgctgcc aacaatcgtc
     2041 gctccgacca gaatctgcgc cattgggcca aattcggtat cgaaaaggca aatcacgcgt
     2101 tcgttacggg caaacagatt cggcacgttc tgagcggtga gatggttaac ggaaaagaga
     2161 tcgcccggca cgtagatcat ctcacgcaga ataccgttgc acggcatgtg tacgcgatgg
     2221 tagtcacgcg gggagaggta agtggtcaca aacgtaccgt tgcggaacag gtccgccatc
     2281 agatagttgc ctgccagcag ggcttcgagg ctgtagttgt ggcctttggc ttgcaggatt
     2341 ttatcttctt cgattttacc caactggctg ataacgccat cggcaggcat gaccagtaca
     2401 ttcggatcgg tatcgattgg gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta
     2461 aaggtgcggt agctggcggt gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat
     2521 ttaacgaaca gatcgataac cagttttgtc agccatcctg cccgcttgct tgcgccccaa
     2581 cccgccaggc gagtaagcca tagtttcggc agaatgtact gtagcgaaag tttaaatgaa
     2641 tttaacaagg tagcctccag gccattgttt tgtcgttcct gatccggcca acatgccgga
     2701 tcctgaaaaa aaggggacga ttctaacgac ggttagctta attgtcagtc atccgtatca
     2761 gaaaagtttt tacgcgtttt tacctgcgcc atgctttcca gaatacggtg atagttttcg
     2821 aaacgggttt ccgcgatttt cccttcctca accgcttccc ggatagcgca gcccggatcg
     2881 gtatcgtgtt tgcaatcgcg atatttacac agacctaaat agtcatggaa ttcgacaaag
     2941 ccctgagtga tttgttccgg ctccaggtgc cagaggccga actcacgcac accaggggaa
     3001 tcaatcacat caccgccgtg cgggaagtga tacagccgag cggcggtcgt ggtgtgctga
     3061 ccgagccccg agttgtcaga gacatcgttg gtcaggatct ctttttgcag ccccagcaac
     3121 gcgttcagca ggctggattt accgacgcca gactgcccgg caaaaatgct gatgcgcccg
     3181 gtcaacgcct cttccagcgg tttcagccca tcctgagtat ggctggaaac catcaatacg
     3241 cgataaccga tgttgcggta gatatccatc tgctcgttga cgaacgccat gccttcgtcg
     3301 tccagcaggt ctatcttgtt gagcacgata atcggctcaa tctgcaaggt ttcgcaggcc
     3361 accaggtaac ggtcgataat attgagcgac agctccggca aaatggcgga gacaatgaca
     3421 atctggtcga tgttggcggc tatgggtttc acgccgtcgt agaaatccgg acgcgttaac
     3481 accgaggtgc gctcatgcac cgcttccacg atccctttga catttacgcc ttccgccgcc
     3541 ggtttacccg gacgccagac tacgcggtcg ccggttacca gcgaacggat ggtacggcga
     3601 atattgcagc ggtgaacgtc gccatcggcg gattccacat cagcgtgcat accaaagcgg
     3661 ctgatgacga taccttcatc aggctcgcca aacagattgt cgtcgtagtc gggcttctcc
     3721 ttagacgttt taagacgacg ctggtgattg gcgttcacgc ggcgctgctg gcctttggag
     3781 agtttatttt tactcaatcg tactgactcc tggtcgcccc tgatgggcaa aacatctatg
     3841 atacacgcaa ttgtggatca atatagtcac tgtgaatggg tggaaaatag catgagtgcc
     3901 aatgaaaaca acctgatttg gatcgatctt gagatgaccg gtctggatcc cgagcgcgat
     3961 cgcattattg agattgccac gctggtgacc gatgccaatc tgaatattct ggcagaaggg
     4021 ccgaccattg cagtacatca gtccgatgaa cagctggcgc tgatggatga ctggaacgtg
     4081 cgcacccata ccgccagcgg gctggtagag cgcgtgaaag cgagcacgat gggcgatcgg
     4141 gaagctgaac tggcaacgct cgaattctta aaacagtggg tgcctgcggg aaaatcgccg
     4201 atttgcggta acagcatcgg acaggaccgt cgtttcctgt ttaaatacat gccggagctg
     4261 gaagtctact tccactaccg ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc
     4321 tggaagccgg aaattctgga tggttttacc aagcagggga cgcatcaggc gatggatgat
     4381 atccgtgaat cggtggcgga gctggcttac taccgcgagc attttatcaa gctgtaaaat
     4441 ttttcctggt cacgtaagcg ccggaatcca cttgcggcgg cgtgattttg acgctaaatc
     4501 gaacgtcttg ctgataattt cagcacttga gataaaaacg caaaaaaaac ttttttgggg
     4561 ggttgcagag ggaaagattt ctcgtataat gcgcctcccg taacgacgca gaaatgcgaa
     4621 aattacgaaa gcaaaattaa gtagta
//
LOCUS       DAJTUN010000190         4644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTUN010000190 DAJTUN010000000
VERSION     DAJTUN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.193"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..937)
                     /gene="uspE"
                     /locus_tag="OO470_003736"
     CDS             complement(<1..937)
                     /gene="uspE"
                     /locus_tag="OO470_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6252832.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDD"
     gene            complement(1089..1841)
                     /gene="fnr"
                     /locus_tag="OO470_003737"
     CDS             complement(1089..1841)
                     /gene="fnr"
                     /locus_tag="OO470_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6252833.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2036..2551)
                     /gene="ogt"
                     /locus_tag="OO470_003738"
     CDS             complement(2036..2551)
                     /gene="ogt"
                     /locus_tag="OO470_003738"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6252834.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2562..2705)
                     /locus_tag="OO470_003739"
                     /pseudo
     CDS             complement(2562..2705)
                     /locus_tag="OO470_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3095..3910)
                     /locus_tag="OO470_003740"
     CDS             complement(3095..3910)
                     /locus_tag="OO470_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6252835.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4007..>4644
                     /locus_tag="OO470_003741"
     CDS             4007..>4644
                     /locus_tag="OO470_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6252836.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLV"
BASE COUNT         1237 a         1085 c         1100 g         1222 t
ORIGIN      
        1 cgtcatccag ttcaacgggt gtctgatact ggtcaggttt aatgaccagc aggtcgcagc
       61 gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc
      121 gtccgaccgt gcccagaacc acaattcccg cctgtaaatg ctccgccaaa tcaggaatca
      181 cctcttcggg cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgcc
      241 gcagggcttt cattgccagc aaatgttgcc cacgaatggc atcgttataa acgctcgggt
      301 caaattccgg cagttcaatc gcgatattaa ttggcgttac cggataagcg ccaaccagat
      361 gaacttcggt atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat
      421 tgagcgcatt atgatacggc tcttcactgg cgagattcac cgccaccagc gccttgcctc
      481 cttccggcca cggctggtct ttcaccatcc acaccgggct tgggcatttg cgtaacagat
      541 gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta
      601 gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat
      661 gccagaccac tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt
      721 ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat agtggtacgt tcgtccgggg
      781 agagcagggt ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc
      841 caccaatccg ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag
      901 gatcgataac aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg
      961 tcaacgcagt ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc
     1021 ttctgccaga tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg
     1081 cggaaaaatc aggcaacgtt acgcgtatga ccagcaagct gggccagcgc atcgttattt
     1141 tcgatggtga tgtatttacc tttgactgcc agcataccgc ttttctggaa gcgacccagc
     1201 agacggctga tggtttctac cgtcaggccc agatagttac caatatcgcc acgagtcatc
     1261 gtcaggcgga attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag
     1321 atgaatgcag ccagacgttc ctcggcattt ttcttcgaca acagcaagat catgtcctga
     1381 tcgcctttga tttcaccgct catcagacgc atcatctgct gacgcagatt cggcatttta
     1441 ccggacaaat cgtccagcgt ttcgaacgga atttcacata ccatcgaggt ttccagcgcc
     1501 tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa atcccactag gtcgcctgct
     1561 aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga ttgtataact tttaatcgta
     1621 ccggagcgga tggcataaag cgatttaagt tcatcaccag ccttaaacag cgtctggcct
     1681 ttctgaatag gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg
     1741 agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg
     1801 ccagactgaa tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa
     1861 ttgatatttg tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac
     1921 agcagagaat tccgatatta gatgtaaata tatgtctatc tatttgaaac tccttaagtt
     1981 gtcaagggta actttacata aaagtgtgaa caagctggca caaattgttt aatgtttaca
     2041 gcaaaagata accttcgtgg cgcaataacc actcttttcg ctgaactccg cctgcatatc
     2101 cggtcatggt gccgtttcgg ccaataaccc gatggcaagg tacgacgatg ctgatgggat
     2161 tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg gcggcccaat tgttcagcca
     2221 gttggccgta atgcattacc tgcccgcagg ggatagtgcg tagtgttttc cagacttcgc
     2281 gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc aataatgcta agattaccgg
     2341 caaaatattc acgaagcttg tcgcttaaac cacctgggtt ggtggcagaa atgcgctcat
     2401 agccttcttt gcgataatgg atgtccagca gctgcaccat gcgttcgctg tactcttccc
     2461 attcaaccgc ccgcaggcga aattgctcat cgcaaatcac ccacagtgga cccagtggcg
     2521 tggcaatttt ttcttcaagt aatctcagca tccgttctct cttaagacaa acgtgggtaa
     2581 atacccggac ctatcggcag accgacaaga taccacgcca gcaacatcag cagccatacc
     2641 accaaaaaga taagtggata gggcaagact aacgaatagt aagtacccag tttcgcgtct
     2701 ggtttgtcgc agtctgatag cataaagcga ttttttaatt tttctgcctg aagaaatgag
     2761 ggcaagccat cacatggatc gagggtggga taattccagt cagtgaaatt ttaccgtagg
     2821 ctatctgtta tttgattctg tggcgtgata gccgccagac accactgaac tcgtcactca
     2881 ttccccaatc ccggcgagaa tgtggttatt tacagagcta aaaaatgaat aaaaaaatag
     2941 agcctgtcga catccgccag actctacagt acacacagca gtgcatcctc gtcttaaatc
     3001 cggcctgggg tcctggtgac cggtgacagt gttataacgg ttttacccat ccgttgccaa
     3061 ttttcttcag tgacagattt cacgaaaata taagttaaat catttgtctt gtgattgcat
     3121 aattatgcat cgttaatcga cgagtaaatt taccgggcga gtagcccgtt cgacgcttaa
     3181 acatattgcc gaacgattca gctgaggcat agcccagacg atgggcaatt ttggtcaacg
     3241 aatatccatc atcaagaagc gtcactgcag aaataacgtg catttgctga acccagtggc
     3301 gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta ctgacgttaa
     3361 ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc aaattctcac
     3421 aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt ttcaccccca
     3481 tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt tgtttatctg
     3541 tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt aacaggggtg
     3601 aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct gaaaccagta
     3661 cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt gggggcagcc
     3721 aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca cgaacgactc
     3781 cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact gtacctccat
     3841 tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg ggttgcagtg
     3901 taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat gttaaatttt
     3961 aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga taatttctaa
     4021 aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg tcttttttat
     4081 ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca ttggtggtaa
     4141 tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg tgcgcgataa
     4201 tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt atcgtgcaaa
     4261 tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc aatctcgaag gcattcaagc
     4321 cactattacg atgcagcagt cgataattca aagtgccagt gaaacatggc aggcagtaaa
     4381 acatgaggag caaaagcgcc tgcgagatac tgaacgttat gaaaaactcg cacaatcagc
     4441 ggcaatatcg caacaaatta ttgataacgc caggtttgac taccagcagg tcgcagcaaa
     4501 ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa cggctggcag ttttatctgc
     4561 tcaggaagaa aacgtacgtg cgagtatcga agaagttcag gccgctttga ctcaggcact
     4621 actggatctg gaatataccc ttgt
//
LOCUS       DAJTUN010000191         4558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTUN010000191 DAJTUN010000000
VERSION     DAJTUN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.194"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..118
                     /locus_tag="OO470_003742"
     CDS             <1..118
                     /locus_tag="OO470_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HCR6252837.1"
                     /translation="VLFTSRDASHYQQGQGTQLLHEIALMLPELLERWIERV"
     gene            115..1011
                     /gene="xerC"
                     /locus_tag="OO470_003743"
     CDS             115..1011
                     /gene="xerC"
                     /locus_tag="OO470_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6252838.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            1011..1727
                     /gene="yigB"
                     /locus_tag="OO470_003744"
     CDS             1011..1727
                     /gene="yigB"
                     /locus_tag="OO470_003744"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6252839.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            1811..3973
                     /gene="uvrD"
                     /locus_tag="OO470_003745"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             1811..3973
                     /gene="uvrD"
                     /locus_tag="OO470_003745"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6252840.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(4120..4551)
                     /locus_tag="OO470_003746"
     CDS             complement(4120..4551)
                     /locus_tag="OO470_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6252841.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         1083 a         1201 c         1293 g          981 t
ORIGIN      
        1 tgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
       61 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
      121 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc
      181 ccgataaccc tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa
      241 aacggcctgc aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc
      301 agtcgccgta aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc
      361 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg
      421 gcaccgaaag cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg
      481 ctggatattg atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg
      541 tacggcgcgg gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg
      601 gagtccggcg aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt
      661 cgcaacgctg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
      721 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
      781 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta
      841 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
      901 ctactgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
      961 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
     1021 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
     1081 accgtccggt gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
     1141 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
     1201 cggaacccga gatttatcac gacgtgacac gttggcgttt tcgttcgatt gaacaagcga
     1261 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta
     1321 actttgccaa atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac
     1381 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
     1441 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
     1501 cgttcagcga tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct
     1561 tacatgttgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
     1621 cttgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
     1681 cacatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
     1741 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
     1801 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc
     1861 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
     1921 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
     1981 tatggctgtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact
     2041 gatgggcacc agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct
     2101 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
     2161 agaccagcta cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
     2221 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
     2281 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
     2341 tcaggaagcg tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca
     2401 cgaactgtgg cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat
     2461 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
     2521 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
     2581 cggggcgcag gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat
     2641 tcgtctggag caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat
     2701 tgaaaacaat aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
     2761 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat
     2821 caaaacctgg caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa
     2881 cgcccagtcg cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta
     2941 cggtgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
     3001 gattgccaac cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
     3061 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
     3121 ctggcaggca tgtcgtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
     3181 cttacagcgg tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct
     3241 gcatgtacag actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga
     3301 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
     3361 ccagttcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca
     3421 tgcggcgctg gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat
     3481 gaccatgcac tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga
     3541 gggcatgttc ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg
     3601 tctggcctac gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
     3661 tcgccgtctg tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc
     3721 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
     3781 gcggatgggt acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg
     3841 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg
     3901 tttgcaggtg gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct
     3961 ggagtcggtg taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat
     4021 tcagattcaa taaattgcaa cgtcatgtag gccggataag gcgtttacgc cgcatccggc
     4081 atcagcagca tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga
     4141 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
     4201 tgaaatcgtg ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
     4261 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
     4321 attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
     4381 aattacaccg ttttccatca acattggtcc tgactgcacc gcaaactgaa tatctttact
     4441 ggctttaaaa gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc
     4501 gccaggacgg ataaagaaat tcccttcacc tgaagcgaga tttaacgcca ccttctgc
//
LOCUS       DAJTUN010000192         4529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTUN010000192 DAJTUN010000000
VERSION     DAJTUN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.195"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            231..1259
                     /gene="torT"
                     /locus_tag="OO470_003747"
     CDS             231..1259
                     /gene="torT"
                     /locus_tag="OO470_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6252842.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1232..1924)
                     /gene="torR"
                     /locus_tag="OO470_003748"
     CDS             complement(1232..1924)
                     /gene="torR"
                     /locus_tag="OO470_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6252843.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2011..3183
                     /gene="torC"
                     /locus_tag="OO470_003749"
     CDS             2011..3183
                     /gene="torC"
                     /locus_tag="OO470_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6252844.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3183..>4529
                     /locus_tag="OO470_003750"
     CDS             3183..>4529
                     /locus_tag="OO470_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6252845.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPI"
BASE COUNT         1097 a         1115 c         1253 g         1064 t
ORIGIN      
        1 ccttgcgcta atgctctgtt acaggtcact aataccatct aagtggttga ttcatagtga
       61 ctggatatgt tgtgttttgt cgcgtttttc agtctattat ttaatcatta agtaatctaa
      121 tgctatgatt tttaaattat ttatgtgatt ttgtcatcct ttaggtgaaa aaggttgagt
      181 cgcaaagtgg aatgcatcta gcataaagcc ttattattga tgaggctatc atgcgcgtac
      241 tgctattttt acttctttcc cttttcatgt tgtcggcatt ttcggctgat aacctgttgc
      301 gctggcatga tgcgcagcat ttcacggtgc aagcctctat gccgcttaaa gccaaacgcg
      361 tatggaaact gtgcgcgctt tatcccagcc tgaaagattc atactggtta tcgttgaact
      421 atggtatgca ggaggctgct cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg
      481 gctacagcca gttggctacc cagcaagcac aaatcgacca gtgtaaacag tggggcgcag
      541 aggccatttt gctcggtagt agcacgacgt catttcccga cctgcaaaag caggtagcaa
      601 atctgccggt gatcgaactg gtaaatgctc ttgatgatcc ccaggtgaaa agccgcgttg
      661 gtgtaccctg gtttcagatg ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg
      721 gtaaaccact gaacgtgctg ctgatgcccg gacccgataa cgccgggggc agtaaggaga
      781 tggtcgaggg ttttcgcgca gccattgccg gaagcccggt gcgtattgtt gatattgcgc
      841 ttggtgataa cgatattgaa atccagcgca acctgttgca ggagatgctg gaacgccacc
      901 cagaaatcga cgtcgttgcc ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc
      961 gtaacctgaa aacgccgctt accgtggtgt cgttttatct ttcacatcag gtgtatcgcg
     1021 ggctgaagcg gggaagagtg attatggctg ccagcgatca aatggtctgg cagggggaac
     1081 tggcagttga gcaggccatc aggcaattac aggggcaatc ggtgtctgat aatgtcagcc
     1141 caccgatttt agttctgacg ccgaaaaatg ccgaccgaga acatatccgc cgctcgctgt
     1201 caccgggggg atttcgtccg gtctattttt atcagcacac atcagcggct aagaaataac
     1261 cttcaccatg ttgcgtcacc agtaaatccg cgctgagttt atgacgtaaa cgacgaatta
     1321 acacatcgac ggtgcgcagg tcagggtttt ccacccgacg cgcagaaagc atacgcagca
     1381 gacgctcacg gctgagaatt tcgcccggat tagtcacaaa tgccaccaac atttcatact
     1441 ctgcgcgggt cagtttaatc ggctcgccat cccgctccag cgtatggcgc gacacattca
     1501 ggcaataacc cgcaaagcga tagcagtagt cctgagtgtg cggttgagct tgtcgcgcga
     1561 ggtcgattcg ccagagcaga tttttcaccc gtactaccag ttcgcgcagt tccagcggtt
     1621 tggtgacgta atcgtctgcg cccatttcca gcccaacaat acggtcaatc cgatcgctgc
     1681 gtccggtaac cagaataatc cccaccgttg agcgttctcg cagggcgcgg gttaacatca
     1741 ggccattttc atcgggtaag ttgatatcca gcagaattaa atctaccggc tgattctgca
     1801 taatttcccg tagcccggca ccgctcgccg taacggaaac ggtatacccc tcctgagtga
     1861 agtaggattg taatcgcgcc tgggtaaccg gcttatcttc aacaataaca atgtgatgtg
     1921 gcatcagagg gttttactca ttctgttcat atctgttcat atttgctcat taagatcgct
     1981 tcactaaacc ataattctac aggggttatt atgcggaaac tctggaacgc gctacgccga
     2041 cccagtgctc gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg
     2101 ctgattgtat tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt
     2161 tgccacagta tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc
     2221 tccggcgtgc gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg
     2281 aagcgcaaac tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat
     2341 acacctgaaa aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg
     2401 aaagaaaaca actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc
     2461 aggcagcatc ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc
     2521 atcgactgtc ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag
     2581 cagttcgatg agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat
     2641 attaagccaa tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct
     2701 tcggaagtga aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg
     2761 acggaaagcg ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt
     2821 gcctcgattc gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc
     2881 gacaccaata ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg
     2941 gtgaacgata tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac
     3001 cagtgccacg gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc
     3061 aacggcatga ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat
     3121 ctgcaaatga atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa
     3181 taatgaacaa taacgatctc tttcaggcat cacgtcggcg ttttctggca caacttggcg
     3241 gcttaaccgt cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg
     3301 cgcaagcggc gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg
     3361 gggctatccg cgcgatggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg
     3421 ataaatatcc gtcgaaaatg atcgccggat tgccggatca cgtacacaac gcggcacgta
     3481 ttcgttatcc gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc
     3541 agcgcggtga taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg
     3601 aagaactgga acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt
     3661 ggcaatcgac ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc
     3721 atggtaatag cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc
     3781 cgcgcgtagt cggttcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc
     3841 agaacagcaa aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact
     3901 ggtggtgccc ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg
     3961 ccggtgaagt tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg
     4021 ggcgcgagca tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg
     4081 cgctggcaca tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact
     4141 gtgtgggttt tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag
     4201 atgccgcatg ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc
     4261 ggcagatggc ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc
     4321 acggtgaaca gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc
     4381 tgccaggtgg tggttttggc tggcactaca acggcgcagg cacgccgggg cgtaaaggcg
     4441 ttattctgag tggtttctcc ggctctacgt cgattccgcc tgttcacgac aacagtgact
     4501 acaaaggcta cagcagcact attccgatt
//
LOCUS       DAJTUN010000193         4498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTUN010000193 DAJTUN010000000
VERSION     DAJTUN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.196"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            67..786
                     /locus_tag="OO470_003751"
     CDS             67..786
                     /locus_tag="OO470_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6252846.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            962..2008
                     /gene="ansB"
                     /locus_tag="OO470_003752"
     CDS             962..2008
                     /gene="ansB"
                     /locus_tag="OO470_003752"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6252847.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            2125..3086
                     /locus_tag="OO470_003753"
                     /pseudo
     CDS             2125..3086
                     /locus_tag="OO470_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(3187..>4498)
                     /locus_tag="OO470_003754"
     CDS             complement(3187..>4498)
                     /locus_tag="OO470_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6252848.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKL
                     ILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLI
                     VGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCS
                     ISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         1361 a         1047 c         1106 g          984 t
ORIGIN      
        1 aacgcgtact ttgttatcaa tctggggcca gcatatgctg gcctgatttg ttcttgaggg
       61 aagactatga tgcgcaaaat gctgctggcg gcagcacttt cagtgacggc aatgaccgct
      121 cacgccgact accagtgcag cgtcacgccg cgtgacgatg tgattgtcag cccgcaaacc
      181 gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc cagacggcaa cgtgatgtat
      241 aacggtaagc aatattccct gaatgccgcc cagcgcgagc aggcgaagga ttatcaggct
      301 gaactacgta gcacgctgcc gtggattgat gaaggcgcga aaagccgcgt cgagaaagcc
      361 cgtattgcgc tggataaaat tatcgttcag gagatgggcg acagcagcaa aatgcgcagc
      421 cgtctgacca aacttgatgc gcagcttaaa gagcagatga atcgcattat cgaaacgcgc
      481 agcgatggcc tgacgttcca ctataaagcc attgatcagg ttcgcgccga aggccagcaa
      541 ctagtgaatc aggcaatggg cggaatttta caggacagca ttaatgaaat gggcgcgaaa
      601 gcggtgctga aaagcggcgg taacccatta cagaatgtgc tgggaagctt gggcggtctg
      661 caatcctcaa tccaaaccga gtggaaaaag caggaaaaag atttccagca gtttggcaaa
      721 gatgtttgta gccgcgttgt gactctggaa gatagccgca aagccctggt cgggaattta
      781 aaataatcct ctattttaag acggcataat acttttttat gccgtttaat tcttcgtttt
      841 gttacctgcc tctaactttg tagatctcca aaatatattc acgttgtaaa ttgtttaacg
      901 tcaaatttcc catgcaaagc taagggataa tgcgtagcgt ccacgtaact ggaggaatga
      961 aatggagttt ttcaaaaaga cggcacttgc cgcactggtt atgggtttta gtggtgcagc
     1021 attggcatta cccaatatca ccattttagc aaccggcggg accattgccg gtggtggtga
     1081 ctccgcaacc aaatctaact acacagcggg taaagttggc gtagaaaatc tggttaatgc
     1141 ggtgccgcaa ctaaaagaca ttgcgaacgt taaaggcgag caggtagtga atatcggctc
     1201 ccaggacatg aacgataatg tctggctgac actggcgaaa aaaattaacg ccgactgcga
     1261 taaaaccgac ggcttcgtca ttacccacgg taccgacacg atggaagaaa ctgcttactt
     1321 cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg gtcggcgcaa tgcgcccgtc
     1381 cacgtccatg agcgcagacg gtccattcaa cctgtataac gcggtagtga ccgcagctga
     1441 taaagcctcc gctaatcgtg gcgtgctggt ggtgatgaac gacaccgtac tggacggtcg
     1501 cgatgtcacc aaaaccaaca ccaccgacgt agcgaccttc aagtctgtta actacggtcc
     1561 tctgggatac attcacaacg gtaagattga ttaccaacgt accccggcac gtaagcacac
     1621 cagcgatacg ccattcgatg tctctaagct gaatgagctg ccgaaagtcg gcatcgttta
     1681 taactacgct aacgcatccg atcttccggc taaagcactg gtagatgcgg gctatgatgg
     1741 catcgttagc gctggtgtgg gtaatggtaa cctgtataaa tccgtgttcg acaccctggc
     1801 aaccgccgcg aaaaacggca ctgcagtagt gcgttcttcc cgcgtaccga cgggtgctac
     1861 cactcaggat gctgaagtgg atgatgcgaa atacggcttc gtcgcctctg gcacgctgaa
     1921 cccgcaaaaa gcgcgcgtcc tgctgcagct ggctctgacg caaaccaaag atccgcagca
     1981 gatccagcag attttcaatc agtactaatc acctcgtccc ggcatcgtgc cggggctttt
     2041 tcacttcaga cttacgtcca ttgccaattt tcattaccct aatgataatc accggaataa
     2101 attattccgc gcgagggttt tcgggtgaaa aagcaatgga ttgttggcac ggcgctgctt
     2161 atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc caactgaaaa tatcttaaaa
     2221 gatcaattca aaaagcagta tcacggcatt ctcaagcttg atgccatcag cttaaaaaat
     2281 cttgatgcta agggtaatca ggccacttgg tcagcggaag gcgatgtctc ttccagtgac
     2341 gatctctata cctgggtcgg tcagttggca gattacgaac tgctcgaaca gacctggacg
     2401 aaagataaac cggtaaaatt ctcggcgatg ttaaccagta aaggaacgcc cgcgtctggc
     2461 tggtcggtga acttttactc ttttcaggcg gcagccagcg atcgtgggcg ggtggtcgac
     2521 gatatcaaaa cgaataataa atatctgatc gtgaatagcg aagatttcaa ttatcgcttt
     2581 agtcagcttg aatctgcgtt gaatacccag aagaactcga ttcctgcgct ggaaaaggaa
     2641 gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag cagcggatgc atattgggga
     2701 aaagatgcaa acggtaagca aatgacccgc gaagaagcct ttaaaaaaat tcaccaacag
     2761 cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg cggttaaata tgacaaagag
     2821 gtttatcaac cggcgattgc ggcatgtcat aaacagagtg aagagtgtta tgaagtgccg
     2881 attcagcaga aacgagattt cgatatcaac gaacaacggc gacagacttt tctgcaatca
     2941 caaaaactca gccgtaaatt gcaggatgac tgggtaacgc tcgaaaaggg caatatccgt
     3001 taacgatgaa agtttcagaa ataaacagta aaaaagtcgc gattttgatg aaaattgacg
     3061 atatcaacca agctaatgaa cgctagaaaa aagataccga acaactgcgg cgcaatgggg
     3121 tgattaagta actcatgtta agtgcctttg ccggataata taaaaagtta tccggcaaat
     3181 tattttttac attagcccta gtagctgtgg aatgaacaag gatatttgcg gaatgtaagt
     3241 gatcatcatc aacgctacta gcaatgcagc ataaaatagc agcaatggtt tgattaaatg
     3301 ttgaatcttc acacccgaga tagaacaacc aacaaatagc gcactaccca ccggcggtgt
     3361 taacagccca atacaaaggt tggccaccat cataataccg aaatgtactg ggtccattcc
     3421 cagctcttgt gcaatcggca agaagatcgg tgtaaagatt agtacagccg gtgtcatgtc
     3481 cataaagatg ccgacaatta ataagacgat attaatcagc agcaatatga tcagtgggtt
     3541 atcagaaatc cccatcaatg cgtcgctgat catataagga atatcagcat tagtcattgc
     3601 ccaggacatg cccacggaaa aaccgatcaa cagtaataca atcgaagtgg taacaaccga
     3661 ctccagaatc agtttgggta aatggcgcca ttttacttcc cggtaaatca gcaccgagag
     3721 aataaatgtg tagacaacgg caattgctga cgcttcagtt gcggtaaaaa tgccgcccag
     3781 aatgccgccc agaatgccgc ccatcacaat gaacaccaga agcaaacttg gtaaggcatc
     3841 cagaaacgct ttacaggcct gagcaaaggt tgctctttct gaaagcgggt agccacggcg
     3901 tttagcaata atgccgcaga ctatcattac cgcaagaccc attaagatcc ctggcaaata
     3961 tcccgccata aacagcgatg ctacagaaac gccgccagct gtcagggaaa acacaattaa
     4021 cacgtttgag ggcggtatca gtaatccagt aatacaagat gagacgttta cagcggtaga
     4081 aaatgccgga tcataacctt ctttggtctg aatgggattc agtgtgccac caacggcagc
     4141 agctgccgca acggctgaac cagaaatgga accaaacatc atattcgcca ggacgttcac
     4201 atgaccaagc gaaccaggaa cgcggccaac cattacctgg gcgaggttaa tcaaacgtat
     4261 cgcaataccg ccgctgttca tcagtgttcc ggcaaagatg aaaaagggga ttgccaacag
     4321 ggcaaagtta tccagtccat tagccagtcg ttgcgaaaca gtgattgccg cgatatccca
     4381 gggaaacatc aaaagcattg aagctacagt ggcaataccg attgagaacg aaatgggcac
     4441 accaataaag accagcacaa aaaaacagcc aaacagtgta atcgcgatat aactatcc
//
LOCUS       DAJTUN010000194         4426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTUN010000194 DAJTUN010000000
VERSION     DAJTUN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.197"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..157
                     /locus_tag="OO470_003755"
     CDS             <1..157
                     /locus_tag="OO470_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6252849.1"
                     /translation="GKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVI
                     GVTFIYE"
     gene            491..2770
                     /gene="ydeP"
                     /locus_tag="OO470_003756"
     CDS             491..2770
                     /gene="ydeP"
                     /locus_tag="OO470_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6252850.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3018..3221
                     /gene="safA"
                     /locus_tag="OO470_003757"
     CDS             3018..3221
                     /gene="safA"
                     /locus_tag="OO470_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6252851.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3289..4050
                     /gene="ydeO"
                     /locus_tag="OO470_003758"
     CDS             3289..4050
                     /gene="ydeO"
                     /locus_tag="OO470_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6252852.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1261 a          944 c          982 g         1239 t
ORIGIN      
        1 tggaaaaata ctggctacgg gagaaaatgt ctccctgggg acagtaaata aaagcaaagt
       61 acctctgggt ttaagcgcaa cctatggaca aacaggaaac aaggtttcag ccggaaccgt
      121 ccagtcagtc attggtgtca cttttatcta tgaataattg tgattttgac cttagtctat
      181 gcgctccatt cacgaaaatt gcgcggataa ttattttgtg aaggctatta tcctacacct
      241 gtaagaaaat ccgcgcacaa ttgaagccag agtcttattt tttatattga aaaatatgtt
      301 aatttaaaca ctatgagaga tgatgcttaa cttatcgtat ttaatctatt gtttaacgat
      361 aatagattaa aaatagaaag agagtagtcc cttactctta tatatccatg ttggcgataa
      421 tccctttgta tgtactgtgc atcatcgcta ttacaaatcc taataattca tttccacaca
      481 ggataagtag atgaagaaaa aaattgaatc ctaccagggt gctgcaggtg gttggggtgc
      541 tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat atacgccatg atgttattgc
      601 catgtttgac atgaataagc cagagggctt tgactgtccg ggttgtgcat ggccagatcc
      661 taagcacagt gcgtcattcg acatttgtga aaacggcgca aaagcaatcg cctgggaagt
      721 cacggataag caggtaaatg cctctttctt tgctgagaat acggttcaat cattacttac
      781 ctggggagat cacgagcttg aggctgcggg gcgtctcact cagcctttga aatatgatgc
      841 cgtcagcgac tgttacaagc cattaagctg gcaacaagct ttcgacgaaa ttggcgcacg
      901 ccttcaaagc tatagtgatc ccaatcaggt tgaattctat acttcgggcc gcacttccaa
      961 tgaagctgcc tttctttatc agctttttgc ccgtgaatac gggagcaata actttcccga
     1021 ctgctccaac atgtgccatg aaccgacaag cgtgggtttg gcagcgagta tcggtgtagg
     1081 taaagggacc gtgttgctgg aagactttga gaagtgcgat ttagtcattt gcattgggca
     1141 taaccctggt acaaaccacc ctcgcatgct gacttcgttg cgcgctttag tgaaacgggg
     1201 agcgaaaatg atcgccatca atcctctaca ggaacgtggc ctggagcgat ttaccgcacc
     1261 gcaaaacccg tttgaaatgc tgacgaactc tgagactcag ttggccagtg cctactacaa
     1321 cgtgcgcatt ggtggcgata tggcgttgct caaggggatg atgcgcctgt taattgagcg
     1381 cgatgatgct gcaagtgccg caggtcggcc ctcattgctt gatgacgaat ttattcaaac
     1441 gcataccgtc ggctttgacg agctacgccg tgacgttctc aattccgagt ggaaagatat
     1501 cgaacgtatt tctggactaa gtcagacaca aatcgccgaa ctggctgacg catatgccgc
     1561 tgccgaacgc accattatct gttacggaat ggggatcact cagcacgaac atggtaccca
     1621 gaacgtgcag caactggtca atttgctgtt gatgaaaggt aacattggca agcctggtgc
     1681 gggtatctgc ccactacgtg gtcactctaa tgtacagggc gaccgaaccg tcggtatcac
     1741 cgagaaaccg tctgcagagt ttctggctcg cctgggtgag cgctatggct tccccccacc
     1801 tcatgcacct ggacatgctg caattgccag catgcaagca atatgtacgg ggcaggctcg
     1861 agcattgatc tgcatggggg gcaactttgc actggcaatg ccagatcggg aagcgagcgc
     1921 tgtaccgtta acgcaattag atttggcggt acacgtagcc actaagctta accgctctca
     1981 tctgctgacc gcacggcata gctatattct gccggttctg gggcgtagcg agattgatat
     2041 gcaaaaaagc ggtgcgcagg cggtaaccgt tgaggattca atgtcgatga ttcatgcctc
     2101 gcgtggcgtg ttaaaacccg ccggtgtaat gctgaaatca gagtgtgcag tggtcgcggg
     2161 aatcgcgcag gcagcactac cccagagcgt ggtagcctgg gagtatctgg tggaagatta
     2221 tgatcgcatt cgcaatgaca ttgaagctgt gctgccagag ttcgccgact ataaccagcg
     2281 catccgtcat cccggtggtt ttcacctgat aaatgcagct gctgaaaggc gctggatgac
     2341 gccgtcaggt aaggctaatt tcattaccag caaagggctg ttagaagatc cctcttcagc
     2401 gtttaacagt aagctggtca tggcgacagt acgcagccac gatcagtaca acacgacgat
     2461 ttatggtatg gatgatcgct atcgaggggt attcggtcaa cgagatgtgg tctttatgag
     2521 tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc ttattgcgct
     2581 tacgccagac ggtaagcgca gctcacgccg catggataga ttaaaagtgg tcatttaccc
     2641 tatggctgac cgctcactgg tgacctattt tccagaatcg aatcacatgc taacacttga
     2701 taaccacgat ccattaagtg gcattcctgg ctataaaagt attccggttg aattagaacc
     2761 atcaaattaa tgcctcttct catttcttct gctgtcatcc gcacagcaga agaattcctc
     2821 attgactatt attgcgcaat ttgctcacat ggattaaatt aaactacata ctataagata
     2881 taaacttctg cctacagctg taagaaactc cgctcagtac tgaagcacca gtcctatttc
     2941 ttcttttctc cagcctgtta tattaagcat actgattaac gacttttaac gttatccgct
     3001 aaataaacat atttgaaatg catgcgacca cagtgaaaaa caaaatcacg caaagagata
     3061 actataaaga aatcatgtct gcaattgtgg ttgtcttatt actgacactt acgttgatag
     3121 ccattttttc ggcaattgat cagctgagta tttcagaaat gggtcgcatt gcaagagatc
     3181 ttacacattt cattatcaat agtttgcaag actggaaata aacagcaaat tataaatgtg
     3241 aaatgtttaa aaggtatcga taaaacttta ttgttttaag gagataaaat gtcgctcgtt
     3301 tgttctgtta tatttattca tcatgctttc aacgctaaca ttttagataa agattacgcc
     3361 ttctccgacg gcgagatcct gatggtagat aacgctgttc gtacacattt tgaaccttat
     3421 gagcggcatt ttaaagaaat cggatttaat gaaaatacca ttaaaaaata tctacaatgc
     3481 actaacatcc agacagtgac ggtgcctgtt cctgcgaagt ttttacgtgc ttcaaatgta
     3541 ccgactggat tgcttaatga aatgattgct tatctcaact cggaagaacg caatcatcat
     3601 aatttttcag aacttttgct tttttcttgc ctgtctattt ttgccgcatg caaaggtttc
     3661 attacactat taactaacgg tgtgctatcc gtttccggga aagtgagaaa tattgtcaac
     3721 atgaagctgg cgcacccatg gaagctgaaa gatatttgtg actgcctgta catcagtgaa
     3781 agcctgttga agaaaaaact aaagcaagag caaacgacat tctcacagat tctattagat
     3841 gccagaatgc agcacgcaaa aaatttgata cgcgtagaag gttcagtcaa taaaattgcc
     3901 gaacaatgtg gttatgccag tacatcttat tttatttatg cgttccgcaa acatttcggc
     3961 aacagtccga agagagtttc taaggagtac cgttgtcaaa ggcacacggg tatgaatacg
     4021 ggcaacacga tgagtgcttt agctatttga ttatttgcta acgagtagtc aaccacgcac
     4081 gctacgtaag aattatatgg ggcagccatt ccctgccccg cgttgttttt aggcgatata
     4141 tttattgaaa taaataagtg acatccatca catatttatg cacttgcata acctgttgca
     4201 tgattattta tgatctcaat tcggcatttt gtcagtaaaa tgcaataatt tattaaatat
     4261 caataaatta gttgtttatc ggcgagaaat tacttaatag aacagaaagt aatgtcaacg
     4321 ctttatggac tgttatttcc ctttttttag ctaaatctgc tatctcttta tgtgactaac
     4381 ttcacttaca tccacttatt tctcttcgta aaattacttc ggaatt
//
LOCUS       DAJTUN010000195         4325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTUN010000195 DAJTUN010000000
VERSION     DAJTUN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.198"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            143..584
                     /gene="tnpA"
                     /locus_tag="OO470_003759"
                     /pseudo
     CDS             143..584
                     /gene="tnpA"
                     /locus_tag="OO470_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(702..1646)
                     /gene="hypT"
                     /locus_tag="OO470_003760"
                     /gene_synonym="qseD"
     CDS             complement(702..1646)
                     /gene="hypT"
                     /locus_tag="OO470_003760"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6252853.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1991..2710
                     /locus_tag="OO470_003761"
     CDS             1991..2710
                     /locus_tag="OO470_003761"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6252854.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2776..4074
                     /locus_tag="OO470_003762"
     CDS             2776..4074
                     /locus_tag="OO470_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR6252855.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1085 a          942 c         1064 g         1234 t
ORIGIN      
        1 cattgtgagt catattcgcc ctgactttcc agaaccatac ggactgcccg ttgacggact
       61 tcgggggaaa aacgagtatt tttagtcatc ctgtttacct ctttctcagg aagtttagtc
      121 tccaggattc ccggggcggt tcatagcgca cacccgatgg aactgtaaat atcacatagt
      181 tttttgcgcc aaaataccga agacaggtgt tctacagaga gaagcgtaga gcaacaggca
      241 gtattttgag aaagctgtgt gagtggaaaa gcgtacggat tctggaagct gaatgctgtg
      301 cagatcatat ccatatgctt gtggagatcc cgcccaaaat gagcgtatcc ggctttatgg
      361 gatatctgaa agggaaaagc agtctgatgc tttacgagca gtttggtgat ttgaaattca
      421 aatacaggaa cagggagttc tggtgcaggg gggactacgt cgatacagtg ggtaagaaca
      481 cggcgaagat acaggattac ataaagcacc agcttgaaga ggataaaatg ggagagcagt
      541 tatcgattcc ctgtccgggc agcccgttta cggaccgtaa gtaacgaagt tggatgcaaa
      601 tgtcagatcg tgtgcgcctg ttagggcgcg gctggtaaga gagccttata ggcgcatttg
      661 aaaaacctcc ggctatgccg gaggatattt attgcacgtc gtcaattgcg gcgtgctggc
      721 tcttccattg atatctgctg gattaaatcg tctggcatat gttcctgtaa aatacgccag
      781 aggttctcgg cggttctatt cagtcgggtg ttcattcggt aaatataagc ctgaatgggg
      841 actgtcaatt tagcagcatc aaggcatatc agacgtttgg tctgtagttc atcgacgatc
      901 gaccagatcg gtaaccaggc aatgccgtat ccgttgagcg ccatattctt taataactcg
      961 ctcatcgaag agataaatat cgtgcgcccg gtaatacctc caacctctgc cagatgacga
     1021 ttgaccaacc tgcccatata agatgtggcg gtatagttga gcaaaggaac ctggggctga
     1081 gtgatatcga atacagggtg cccctgtgcg tcggcggcac ataccgggta caactcagat
     1141 tcaaacagtt tcaggcagca aaaaggcgat tgcataagat cttcatcgcg aaacgaaata
     1201 ataaagtcac tcttgccttc gcgtaacgta tttacggcct ggacaacatc aatggcttca
     1261 acatgataga caaattctcc gccataggcg gtaagtgaat gcaccagttt gggtaatacg
     1321 ctcagggata gtgagtgcgc ggctgcaatt ttaatgttgg gaacgccgag taaactctgg
     1381 ccgtttaact cccccaaatt gcattcaagc tgctgtaata agctgcgagt ctgggaatga
     1441 aagagcttgc cctcttcggt taattgtaaa ggggtggtcg tgcgatcgaa taaaacaacg
     1501 ccgatcgctg cctccagcgc cttaattcgt cggctgaatg cgggttgcga aagattacgc
     1561 tcttttgccg cctgagaaaa atggcgacag gcttccagtg tcaggaaatc gtataaccat
     1621 tttgtctcga tattcttcat taccatggca cctgtatttt caatccaaag acataataaa
     1681 acatcccggc aataaaaata atgttctcaa ttagcacaac ctggccttta tcattcgttt
     1741 tgtgcataac cccttcattt ccggtattga cgttgatatc acatttcgcc aaaaaaacga
     1801 aatttttccg tcagataacc agaatacatc atcactatgg tgaataagcg gaatgttcca
     1861 tagctgcatc atgcgtgtct tgcatcgcct tgtacaacct tgcatttctc atcgcccatt
     1921 tcgactgtta aaaaagagag cagcagttat ttaccgcgta ttgtcattaa cgttttgaga
     1981 ggcaggtatt gtgaaaggtt taattggtgt actcggtgga atggggccgg cagctaccgt
     2041 cgacctgttt aataaatttg taacttttac cgctgcacaa tgtgatcagg aacatattcc
     2101 tttaattatt tcttcgatcc cggatattcc tgaccgtacc gatgcattaa tgcatcatgg
     2161 gcgttcacca ttacccgtta tgcgtgatta tatgcataag ctggaagatg ccggggcaga
     2221 atgtattgtt attccctgta ataccgcgca ttactggttc aaagaattaa aagatgcttg
     2281 tcatattgat atattaagta tcgtagaaac tacgatcaat gaagttcgcg cctgtggtaa
     2341 aacccgaatt ggcttgttag ccaccaatgc cacgctatat atgggtttat accaaaaagg
     2401 aattgagtcg ctgggattta cctgtgtcag ccccgatgcc gacggtcaga aaaaagtgat
     2461 ggaaagtatt tattccttga aagctggcaa tatagcgcac gcacaaaagt tgatgaatga
     2521 gcaggctgaa atattatttt cccgcggtgc agaaatcctt gttcttggtt gtacagaaat
     2581 tccggttatt ctggcgcaag ccgttaaaga gcagccttcc cgctatattg actcaacggc
     2641 gtcactcgtt cgtgccggaa taaaatggta cgaaaatcgt gtcggtaaac attatctttt
     2701 aacccaataa tcatttaaat cgcagccaga aagttattcg cttttaactg aattatattt
     2761 ataacggaga acattatggt ttggctggaa attatcgtag tacttggtgc aatatttttt
     2821 ggtattcgcc aggggggaat cggtattggt ttatgtggcg ggcttgggct tgccattctg
     2881 actctgggat ttggtctgcc tatggggtcg ccgccagtcg acgtgatcct gattattatg
     2941 accgttgttg tggcggcctc agccttacag gccgcaggcg gaatggatta tctggtacgc
     3001 gtcgctggta attttatgcg gcgtaacccg aaatatatca acataatcgc gccgattatc
     3061 acctggtcta tgaccattat ggccggtact gggtttattg ttttttccac gctgccggtg
     3121 attgctgaag tggcgaaaga gtccggtatc cgtccttccc gcacgcttgc cggttcggtt
     3181 gtcgcttcgc aggttgcgat ttccggttcg ccgatcagcg ccgcaatggc ggctatgctc
     3241 actattatgg aggtaaacgg tgtcagtttt attcaagtga tgtcggtatg tcttcccacc
     3301 tcttttgctg ccgcgatggt cgccgctttt attgcttccc gacagggatg tgagttgcag
     3361 gatgacgagg tctaccttga gcgcctgcaa aaaggactgg tgcaaaaata cgagaataat
     3421 agcagcatca agccaggtgc gacgctctcc gtcggattgt tcctgctggc gacggtcgct
     3481 attgttattc ttgctgcctt cccccaactg cgtccgggtt ttgatatcgg taaaccgatg
     3541 gaaacacgcg atatcattat tatctgtatg ctctctgccg cctgcctgat ggtggtgctg
     3601 tgtaagacgg cgaccgatgc cattattctc acctccacat tccgtgcggg gatgagttcg
     3661 ctggcggtaa ttctcggcat tgtaacctta ggcaccactt ttattgatgc gcatttaact
     3721 gagattaaag atattgctgg tgatattttg caggcgtatc cgatgttgtt ggcgctggtt
     3781 ctttttggca cttgcgcgct gctctactcg caaggtgcga ccacgccgtt aattatcccg
     3841 cttgccgtcg cgcttgacgt gcccacctgg gcaattctgg cgtcctatgt ggccgtcacc
     3901 ggagtatttg tactgccaac gtatccaaca tcactggctg ccatggaatt tgatactacc
     3961 ggcacaactc gtgtaggcaa atatgtgctg aatcatccat ttatgctgcc tgggttgggg
     4021 ggaattattg ctggagtggc attaggcttt gtgattgcgc caatgattat ttagcactat
     4081 caaacatcag tatcgatggt ttatacacaa taaagacaga tatggcggaa tatagtctgt
     4141 tgtaagcgcc aacaaagaat aggggaattc cgccatgtct gaacatcttc acaatatggt
     4201 attaagtgtt gtcgtttaat gatttatttc ttggttatgt tgtgtattag ctgcatctat
     4261 cgctttttga tacctgaggt gtactggcaa tagcggacac taccatttgt tcttttttta
     4321 agcag
//
LOCUS       DAJTUN010000196         4295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTUN010000196 DAJTUN010000000
VERSION     DAJTUN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.199"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(139..1041)
                     /locus_tag="OO470_003763"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="OO470_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="OO470_003764"
     CDS             1101..1379
                     /locus_tag="OO470_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252856.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="OO470_003765"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="OO470_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6252857.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="OO470_003766"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="OO470_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6252858.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="OO470_003767"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="OO470_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6252859.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..>4295
                     /gene="hdeB"
                     /locus_tag="OO470_003768"
     CDS             3981..>4295
                     /gene="hdeB"
                     /locus_tag="OO470_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6252860.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNENDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SND"
BASE COUNT         1266 a          923 c          899 g         1207 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaacgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgac
//
LOCUS       DAJTUN010000197         4288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTUN010000197 DAJTUN010000000
VERSION     DAJTUN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.200"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..935
                     /gene="tauA"
                     /locus_tag="OO470_003769"
     CDS             <1..935
                     /gene="tauA"
                     /locus_tag="OO470_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6252861.1"
                     /translation="AALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVD
                     WRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKK
                     TISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDI
                     DGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSA
                     IDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPV
                     NKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            948..1715
                     /gene="tauB"
                     /locus_tag="OO470_003770"
     CDS             948..1715
                     /gene="tauB"
                     /locus_tag="OO470_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6252862.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1712..2539
                     /gene="tauC"
                     /locus_tag="OO470_003771"
     CDS             1712..2539
                     /gene="tauC"
                     /locus_tag="OO470_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6252863.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2536..3387
                     /gene="tauD"
                     /locus_tag="OO470_003772"
     CDS             2536..3387
                     /gene="tauD"
                     /locus_tag="OO470_003772"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6252864.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3493..>4288)
                     /gene="hemB"
                     /locus_tag="OO470_003773"
     CDS             complement(3493..>4288)
                     /gene="hemB"
                     /locus_tag="OO470_003773"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6252865.1"
                     /translation="LAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARMSR
                     ICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGADFI
                     APSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKSYQ
                     MNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEYAM
                     IKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT          934 a         1139 c         1268 g          947 t
ORIGIN      
        1 ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc gtggcgtatc
       61 aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct aaagagagcg
      121 gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg gcgctggctt
      181 ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca gccagccaac
      241 aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa gcgctggtgg
      301 taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc gtaccgttta
      361 tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt aaaccagggc
      421 tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag cggggtgata
      481 ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac ggcaaggtgc
      541 tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc tgggtggtgc
      601 gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa agcgccatcg
      661 atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg gaaaacatca
      721 gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg gtgaagggaa
      781 atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg aacaaagcaa
      841 tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta gcgaatgatt
      901 acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg ctgcaaatct
      961 ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac ctgacgctgg
     1021 aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc accctgctga
     1081 atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg ggtaagcgta
     1141 ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg ctaccgtggc
     1201 gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag aaaatgcagc
     1261 gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca gaaaaacgct
     1321 atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc gcgctggcgg
     1381 cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc ttcacccgcg
     1441 accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag gtgctgttga
     1501 ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg ctttcatccg
     1561 gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt gttgcgggag
     1621 agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa tatgttttaa
     1681 gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg aaaaactgca
     1741 ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa gcattggcac
     1801 gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga ttagcccgct
     1861 gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg gaccgcaggg
     1921 atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca ttgtgctggc
     1981 gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc taagcccgac
     2041 ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac cgctggctta
     2101 tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct tactgatcta
     2161 tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa gcgcgcagca
     2221 ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc tgtggtttgt
     2281 cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc tgggtgtggg
     2341 ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag gatttatggt
     2401 tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg cggtgattgc
     2461 gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc tgacgccctg
     2521 gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc gtatatcggc
     2581 gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt tgaacagctt
     2641 taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat tacgccgcag
     2701 cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt ttaccctcat
     2761 gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc gccggataac
     2821 gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc gattctggca
     2881 gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat tgcggcctat
     2941 gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga gcatgatttc
     3001 cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca acgctggcgc
     3061 gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca tccggtgagc
     3121 ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga tgtcagcgag
     3181 aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc ggagtttcag
     3241 gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt gactcagcac
     3301 tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac gatccttggg
     3361 gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg cccccttggg
     3421 gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc cccctttctg
     3481 agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa agtagctgaa
     3541 aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga ctttctcttc
     3601 atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc tcacctgata
     3661 cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga ggtacgctcc
     3721 agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg actcacgaat
     3781 cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt ttaatgcgct
     3841 tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg aatacgacat
     3901 aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct gtacctggcc
     3961 gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa ccacggcttg
     4021 cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca gcacaccgca
     4081 gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca tttctggcac
     4141 agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc aggcgtcgct
     4201 gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga taccggcgtt
     4261 ggcgatgcgt tcaatttcgc gtgccaga
//
LOCUS       DAJTUN010000198         4222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTUN010000198 DAJTUN010000000
VERSION     DAJTUN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.201"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            19..900
                     /gene="yddG"
                     /locus_tag="OO470_003774"
     CDS             19..900
                     /gene="yddG"
                     /locus_tag="OO470_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6252866.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1155..2255
                     /gene="ompC"
                     /locus_tag="OO470_003775"
     CDS             1155..2255
                     /gene="ompC"
                     /locus_tag="OO470_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6252867.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2419..2904
                     /locus_tag="OO470_003776"
     CDS             2419..2904
                     /locus_tag="OO470_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6252868.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3014..4003
                     /locus_tag="OO470_003777"
     CDS             3014..4003
                     /locus_tag="OO470_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6252869.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1308 a          870 c          801 g         1243 t
ORIGIN      
        1 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
       61 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggcccggtc
      121 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
      181 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
      241 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
      301 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
      361 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
      421 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
      481 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
      541 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
      601 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
      661 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac tgcatggaat
      721 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
      781 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
      841 ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
      901 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
      961 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     1021 aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt aaaaacagta
     1081 tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa cgataattaa
     1141 aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt ttgttagctg
     1201 cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc gacctttatg
     1261 gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt gataaaactt
     1321 atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt ggttttggtc
     1381 actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc ggcaacaaga
     1441 cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg ctctctggac tatggtcgta
     1501 actacggtgt cggctacgac attggttcat ggaccgatat gttgccagaa tttggtggcg
     1561 atacctggag tcagaaagat gtcttcatga cataccgtac caccggtcta gcaacctatc
     1621 gcaactacga tttctttggc ttaattgaag ggctgaactt tgccgcgcaa tatcaaggca
     1681 aaaatgaacg tactgacaac agtcatcttt atggtgctga ctacacgcgt gccaacggtg
     1741 acggtttcgg tatctcctca acttatgttt atgatggctt tggtatcggt gcggtatata
     1801 ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc taatcctctc aatgcctccg
     1861 gtaagaatgc agaactatgg gccacaggta taaagtatga tgccaacaac atctactttg
     1921 cagctaatta cgatgaaaca ttaaacatga ccacctatgg ggatggttat atctctaaca
     1981 aagcacaaag ttttgaagtg gtggcgcaat atcaattcga cttcggcttg cgcccatcac
     2041 tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag gacatgattg
     2101 agtatatcga cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc tatgttgatt
     2161 ataaaatcaa cctgattgat gaaagcgact ttacccgtgc cgtagatatt cgcaccgata
     2221 acatcgtcgc tacgggcatt acctatcagt tctaacgtcc tctcctgacc agcccttctc
     2281 tgggctggtt tttgatttaa gtgctgctgt atgactcctt aagtaaaaat gagcaattct
     2341 tgatatttct ggtcattcac ctcaattaat attattccat atcctccggg gacatcttac
     2401 tataatattt attgatgagt gattatgacc aatattaata cagcttgtgt aaaaaataat
     2461 gccagttatc aacttaataa cgcattacct aacaaagaaa ccatctccag caatttttgt
     2521 gaacgactag aacaatgggg taataaatcg gttaataatg gtgaagaaag agcaattgcc
     2581 gtggagcgaa ttaaagaagc ttacaattcg aacatggcat ctcttgattt gtcatatctt
     2641 gatttgagtg aattaccgcc tatcccctcg acagttaata cgttaaatct ggaaaacaat
     2701 tgtctaactt gtcttgactt agctgacaat gccagtctcg ttaatatcaa cctcagcgtt
     2761 aataaaatta acacgataac ctttccaaaa gaaccaaaac tggaaaacat ttatattgat
     2821 cataacaatc tggaaagttt agatttaaaa aatcagcatt cattggttaa tctggaagcg
     2881 caaaataata gcctaaaaaa atgatatttc tgatagttat aaactgaaat ttcttaatct
     2941 tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga ttgagttaag
     3001 tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga tgaaaacaat
     3061 cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa aactggaata
     3121 tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac taatatcatc
     3181 aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg cacaaatgca
     3241 gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca ttgatttatc
     3301 aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacctcta tcaatcttga
     3361 taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa agctaagtct
     3421 gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa gtattacaca
     3481 tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt catcagttac
     3541 ttattttagt gcggcacata atcaactaga gtttgtgcaa ttagaatctt gcgaatggct
     3601 gcaatacctg aatctcagcc ataatcaatt aactgatatt gttgcaggta ataaaaatga
     3661 actcttactg ctggatctat cccataataa actaacaagt ttacacaatg tcttatttcc
     3721 caacttgaat acgttactta ttaacaacaa tttgctttct gaaattaaaa tattctatag
     3781 caacttccgc aatgttcaga cattaaacgc tgctaacaac cagttgaaat atataaatct
     3841 tgatttcctg acttatcttc catctatcaa aagtttaaga ctggacaata ataaaataac
     3901 ccacattgat actaataata catccgatat tggaacttta ttccccataa taaaacagag
     3961 caaaaactta aattttttaa atgtttctgg gaagaacaat tgacctacta tgcagctcat
     4021 gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa ttctttcgcc
     4081 tggtgcattt gaagttcact ctgacggaat agatgcggat aacgaattgt ttcactatac
     4141 tattaaaaaa gcatataccc catataatat acacacttac aagacagagg aagttgtaaa
     4201 ccagaggaat ataaaagtta aa
//
LOCUS       DAJTUN010000199         4197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTUN010000199 DAJTUN010000000
VERSION     DAJTUN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.202"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            475..2760
                     /gene="nrdA"
                     /locus_tag="OO470_003778"
     CDS             475..2760
                     /gene="nrdA"
                     /locus_tag="OO470_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6252870.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2994..4124
                     /gene="nrdB"
                     /locus_tag="OO470_003779"
     CDS             2994..4124
                     /gene="nrdB"
                     /locus_tag="OO470_003779"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6252871.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4124..>4197
                     /gene="yfaE"
                     /locus_tag="OO470_003780"
     CDS             4124..>4197
                     /gene="yfaE"
                     /locus_tag="OO470_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6252872.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAA"
BASE COUNT         1061 a         1119 c         1041 g          976 t
ORIGIN      
        1 aattaacaaa atggcttcgc atttaacaat aaccgaatag aaaacaacca tttcgacatc
       61 aacaatcgct tacattcgct tatatattga ccacaactga tacatcagat tatgtgatga
      121 ctcgtgctta gatcaatttt tgcaatcatt agcaaaaaga ttaataaacc atctatatca
      181 atttatccaa cctattctgc cgttcaagaa atcgccgaac agttattttt aacaaatttt
      241 tctcttccca ttgactttcc cggacacctt gtctgaccta aggtgcgcga aagccacttt
      301 ttccttcctg agttatccac aaagttatgc acttgcaaga gggtcatttt cacactatct
      361 tgcagtgaat cccaaacata ccccctatat atagtgttct aagcagcttc ccttactaca
      421 ggtagtctgc atgaaactat tgcggaaaga attccaaaaa caggtacgac atacatgaat
      481 cagaatctgc tggtgacaaa gcgcgacggt agcacagagc gcatcaatct cgacaaaatc
      541 catcgcgttc tggattgggc ggcagaagga ctgcataacg tttcgatttc ccaggtcgag
      601 ctgcgctccc acattcagtt ttatgacggt atcaagacct ctgacatcca cgaaactatt
      661 atcaaggctg ccgcagacct gatctcccgt gatgcgccgg attatcagta tctcgccgcg
      721 cgcctggcga tcttccacct gcgtaaaaaa gcctacggcc agtttgagcc gcctgcgctg
      781 tacgaccacg tggtgaaaat ggtcgagatg ggcaaatacg ataatcatct gctggaagac
      841 tacacggaag aagagttcaa gcagatggac acgtttatcg atcacgaccg tgatatgacc
      901 ttctcttatg ctgccgttaa gcagctggaa ggcaaatatc tagtacagaa ccgcgtgacc
      961 ggcgaaatct acgaaagcgc ccagttcctt tatattcttg ttgccgcgtg cctgttctcg
     1021 aactacccgc gtgaaacgcg cctgcaatac gtgaagcgtt tttatgacgc ggtttccaca
     1081 tttaaaattt cgctgccgac gccaatcatg tccggcgtgc gtaccccgac tcgtcagttc
     1141 agctcctgcg tactgatcga gtgcggtgac agcctggatt ccatcaacgc cacctccagc
     1201 gcgattgtga aatatgtttc ccagcgtgcc gggatcggca tcaacgccgg acgtattcgt
     1261 gcgctgggca gcccgattcg cggtggcgaa gcgttccata ccggctgtat cccgttctac
     1321 aaacatttcc agacagcggt gaaatcctgc tctcagggcg gtgtgcgcgg cggtgcggca
     1381 acgctgttct acccgatgtg gcatctggaa gtggaaagcc tgctggtgtt gaaaaacaac
     1441 cgtggggttg aaggcaaccg cgtgcgtcat atggactacg gggtacaaat caacaaactg
     1501 atgtataccc gcctgctgaa aggtgaagat atcaccctgt tcagcccgtc cgacgtaccg
     1561 gggctgtacg acgcgttctt cgccgatcag gaagagtttg aacgcctgta taccaaatat
     1621 gagaaagacg acagcatccg caagcagcgt gtgaaagccg ttgagctgtt ctcgctgatg
     1681 atgcaggaac gtgcgtctac cggtcgtatc tatattcaga acgttgacca ctgtaatacc
     1741 catagtccgt ttgatccgac catcgcgcca gttcgccagt ctaacctgtg cctggaaatt
     1801 gccctgccga ccaaaccgct gaacgacgtc aacgacgaga acggtgaaat cgcgctgtgt
     1861 acgctgtctg ctttcaacct gggcgcaatt aatagcctgg atgaactgga agagctggcg
     1921 attctggcgg ttcgtgcact tgacgcgcta ctggattatc aggattaccc gatcccggcc
     1981 gccaaacgtg gagcgatggg gcgtcgtacg ctgggtattg gtgtgattaa cttcgcttac
     2041 tatctggcga agcacggtaa acgctactcc gacggcagcg ccaacaacct gacgcataaa
     2101 accttcgaag cgattcagta ttacctgctg aaagcgtcta atgagctggc taaagagcaa
     2161 ggcgcgtgcc cgtggtttaa cgaaaccact tacgcgaaag ggatcctgcc gatcgatacc
     2221 tataagaaag atctggatgc catcgctaat gagccgctgc attacgactg ggaagcattg
     2281 cgtgaatcaa tcaaaacgca cggcctgcgt aactccacgc tttctgccct gatgccgtcc
     2341 gagacctctt cgcagatctc taacgccact aacggtattg aaccgccgcg cggttacgtc
     2401 agcatcaaag cgtcgaaaga cggtattttg cgccaggtgg tgccggacta cgagcacctg
     2461 cacgacgcct atgagctgct gtgggaaatg ccgggtaacg atggctacct gcaactggta
     2521 ggtatcatgc agaaatttat cgatcagtcg atctctgcta acaccaacta cgatccgtca
     2581 cgtttcccgt caggaaaagt gccgatgcag cagttgctga aagacctgct caccgcctac
     2641 aaattcgggg tcaaaacgct gtattatcag aacacccgtg acggcgctga agacgcgcag
     2701 gacgatctgg taccgtcaat ccaggacgat ggctgcgaaa gcggcgcatg taagatctga
     2761 tattgagatg ccggatgcgg cgtaaacgcc ttatccggcc tacggctcgg tttgtaggcc
     2821 tgataagacg cgccagcgtc gcatcaggct ccgggtgccg gatgcagcgt gaacgcctta
     2881 tccggcctac ggctcggatt tgtaggcctg ataagacgcg ccagcgtcgc atcaggcaca
     2941 ggatgcggcg taaaatgcct tatccggcat taaactccca acaggacaca ctcatggcat
     3001 ataccacctt ttcacagacg aaaaatgatc agctcaaaga accgatgttc tttggtcagc
     3061 cggtcaacgt ggctcgctac gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa
     3121 agcagctctc tttcttctgg cgtccggaag aagttgacgt ctcccgcgac cgtatagatt
     3181 accaggcgct gccggagcac gaaaaacaca tctttatcag caacctgaaa tatcagacgc
     3241 tgctggattc cattcagggt cgtagcccga acgtggcgct attgccgctt atttctattc
     3301 cggaactgga aacctgggtc gaaacctggg cgttctcaga aacgattcat tcccgttcct
     3361 atactcatat cattcgtaat atcgttaacg atccgtctgt tgtgtttgac gatatcgtca
     3421 ccaacgagca gatccagaaa cgtgcggaag ggatctccag ctattacgat gaactgatcg
     3481 aaatgaccag ctactggcac cttctgggcg aaggcaccca caccgttaac ggtaaaaccg
     3541 taaccgttag cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg
     3601 cgctggaagc gatccgcttc tacgtcagct ttgcttgttc ctttgccttt gcagaacgcg
     3661 aactgatgga aggtaacgcc aagattattc gcctgattgc ccgcgatgaa gccctgcacc
     3721 tgaccggtac tcagcatatg ctgaatctgc tgcgcagcgg cgcggatgat ccggagatgg
     3781 cggaaattgc cgaagaatgt aagcaggagt gctatgacct gtttgtccag gcggcacagc
     3841 aagagaaaga ctgggcggat tatctgttcc gtgacggttc gatgattggt ctgaataaag
     3901 atattctctg ccagtacgtt gaatacatca ccaatatccg catgcaggca gtcggtttgg
     3961 atctgccgtt ccagacgcgc tccaacccga tcccgtggat caacacctgg ctggtgtctg
     4021 ataacgtgca ggttgctccg caggaagtgg aagtcagttc ttatctggtc gggcagattg
     4081 acgcagaagt ggacaccgac gatttgagta acttccagct ctgatggccc gcgttaccct
     4141 gcgcatcact ggcacacaac tgctgtgcca ggatgaacac ccttcccttc tggcggc
//
LOCUS       DAJTUN010000200         4190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTUN010000200 DAJTUN010000000
VERSION     DAJTUN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.203"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..417
                     /locus_tag="OO470_003781"
     CDS             <1..417
                     /locus_tag="OO470_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR6252873.1"
                     /translation="KNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEM
                     GAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMGRN
                     IFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            441..731
                     /gene="lsrG"
                     /locus_tag="OO470_003782"
     CDS             441..731
                     /gene="lsrG"
                     /locus_tag="OO470_003782"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6252874.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            770..1528
                     /gene="tam"
                     /locus_tag="OO470_003783"
     CDS             770..1528
                     /gene="tam"
                     /locus_tag="OO470_003783"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6252875.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1532..2446)
                     /locus_tag="OO470_003784"
     CDS             complement(1532..2446)
                     /locus_tag="OO470_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6252876.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            2647..2835
                     /locus_tag="OO470_003785"
     CDS             2647..2835
                     /locus_tag="OO470_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252877.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            2845..4043
                     /locus_tag="OO470_003786"
     CDS             join(2845..3661,3661..4043)
                     /locus_tag="OO470_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6252878.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDIS
                     LLRYMPA"
BASE COUNT         1066 a         1013 c         1179 g          932 t
ORIGIN      
        1 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
       61 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
      121 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
      181 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
      241 gagacgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
      301 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
      361 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
      421 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
      481 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
      541 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
      601 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
      661 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
      721 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
      781 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
      841 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
      901 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
      961 tccggcgatg attgctgaag ctcgcagtgc tttgccagac tgccagttcg tggaagcaga
     1021 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     1081 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     1141 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     1201 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     1261 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     1321 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     1381 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     1441 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     1501 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     1561 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     1621 tgcagatggc atccagcggt aaatcattgt tttcagtacc gaacggttct tccagttctt
     1681 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     1741 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     1801 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     1861 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     1921 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     1981 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     2041 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     2101 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     2161 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     2221 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     2281 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     2341 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     2401 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     2461 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     2521 ttacctagaa ccacggcagc ctgacggctg caaattgaaa actgcccctg ttcgtcctgt
     2581 tgttgccgca cgctccgcag aatatgtatg ctctgaggac ctgttttatt aaggactcat
     2641 cctcttatgt tgacccgcaa aagtatagat accgttctgc tctctgttgg tgctgagaag
     2701 ctctcacaac gtgaatggga ctggatgaag atgctcaaac ccatggaccc accacctgcg
     2761 atggtcgcgg cctccatcct ggagcgccgt ggcgacacag cagcccttac ccggctacag
     2821 gataccgggg gttaatccgt cctcatgttg tccgctttta ctccccgccc gctgaaacgt
     2881 ctgttcacgg ccaaccagtg ctggacatcc ttcctggatg cgggcggtct gcgcgatatc
     2941 gaggttgagg ctgtcacaaa aatgctggcc tgcggcacgc ggatactggg cgtgaaggaa
     3001 tacatctgcg ataaacctga gtgcccccac gtcagatacg tcactaactc atgcggcagc
     3061 cgtgcctgcc cgtcctgcgg aaaaaaggcc acagacctgt ggatagcgac acagctgaat
     3121 cgtcttcctg actgcgactg ggtacacctg gtcttcaccc tgccggacac gcagtggccg
     3181 gtgttcgaaa gcaaccggtg gctgctgaat gacgtgtgcc gtctggcggt ggagaatctg
     3241 ctgtatgccg cccggaaacg ggggcaggaa cccggtatct tctgcgccat ccacacgtat
     3301 ggccgtcgtc tcaactggca tccgcatgta catgtgtctg taacctgtgg aggtctgaat
     3361 aagcatggtc agtggaaaaa gctgagcttc ctgaaagacg cgatgcgttc gcggtggatg
     3421 tggaatatgc ggcaacggct tctgaaagcg tggtcagagg ggctggcgat gccggagtcg
     3481 ttgtcacata tcacgacgga atcacagagg agaagcctgg tgctgaaagc cggaggaaaa
     3541 tactggcatg tgtacatgtc gaaaaaaacg gccggcggga ggaatacggc gcgctacctg
     3601 ggtcgttatc tgaagaagcc gccgatagcg gcctcccggc tggcacatta caacggaggg
     3661 ggagcctgag cttccgttac ctggaccaca aaacgggaga aacggcgacg gaaacgctga
     3721 cacagcgtga gctggtcgcg aggctgaaac agcacatccc ggagaagttt tttaagatgg
     3781 tgaggtactt cgggttcctt gccaaccggg tgtgtggaga gaagctgccg caggtgtacc
     3841 gtgctctggg gatggataaa ccggaaccag tggcgaaagt gtgctatgca caaatggtga
     3901 aacagttcct gagtcgtgac ccgttcgaat gcgtgctgtg tggcggccgg atggtatacc
     3961 gccgggccat cgcgggactg aatgtggagg ggctgaagaa aaacgcgcgg gatatcagtc
     4021 tgctgaggta tatgccggcc tgacacaggc taagtgtgcc cgggaatcgg tgcaagtata
     4081 aaaaacagcc tgttgcggta taagaatcga ccatgcagag agagaaaaca ccggagtgga
     4141 gggaaaagca gaaaagctca cgtggcataa ggcgtggaca gcggtatcgt
//
LOCUS       DAJTUN010000201         4185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTUN010000201 DAJTUN010000000
VERSION     DAJTUN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.204"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(33..536)
                     /gene="cheW"
                     /locus_tag="OO470_003787"
     CDS             complement(33..536)
                     /gene="cheW"
                     /locus_tag="OO470_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6252879.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(557..2521)
                     /gene="cheA"
                     /locus_tag="OO470_003788"
     CDS             complement(557..2521)
                     /gene="cheA"
                     /locus_tag="OO470_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6252880.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(2526..3452)
                     /gene="motB"
                     /locus_tag="OO470_003789"
     CDS             complement(2526..3452)
                     /gene="motB"
                     /locus_tag="OO470_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6252881.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3449..>4185)
                     /locus_tag="OO470_003790"
     CDS             complement(3449..>4185)
                     /locus_tag="OO470_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6252882.1"
                     /translation="KAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMF
                     SLERDIENPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIET
                     HESEAEVPANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFL
                     GILLAYGFISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSE
                     RPSFIELEEHVRAVKNPQQQTTTEEA"
BASE COUNT          927 a         1169 c         1034 g         1055 t
ORIGIN      
        1 cgggttcatt tcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct
       61 aacagcgcca tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatacgg
      121 tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc aaactctggt
      181 gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg aaacgccgtc aaccacgatg
      241 ccgaccaccc gctgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca
      301 tccacctggc tgaacttaat tcgtaagtca acaatcggca caataacgcc gcgcagattc
      361 gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg
      421 atctcctgca ctttcaggat atcaataccg tactcttcat caccaagggt aaataccaga
      481 aattcctggc ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg
      541 ttaccttttt actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct
      601 gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag
      661 aaatgccggg gactttgcga tagttacttt caaggttttt aaccacaacc tggtgttgac
      721 caattaattg atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa
      781 ttccctgggt ggcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga
      841 tgggcagata ttcaccccgc acttccagca cccgctcgcc gccggccagt ggatggagat
      901 cggcttcacg gggttgcagt gattccataa cagcattcag cggcagaatg aaaacttcat
      961 ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga
     1021 tcgtagtgcc agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat
     1081 tacgtttaac gacgtccatg ccgacgccgc gcccggagac gtcggtgacc tgctctgccg
     1141 tggagaagcc tggtgcaaat atcagcattg caacttcgtc gtcgctcatg ttttcgctga
     1201 cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc acggtttagc cccgccccat
     1261 cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc
     1321 cgacgctgtt tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc
     1381 tattgcgtac caggtgggtc agcgggtcga taatgcgttc tatcaggctc ttgtcgagct
     1441 cggtggaact gcccaccagc gtcagttcta cctgcttgcc gagttttccc gccagatcac
     1501 gcaccagccg gggatagcga ctaaagacat attccatcgg catcatgcga atcgacatca
     1561 ctgattcctg caaatcacgg gcgttacgtt gtaactgccc catgctggta atcaaatcac
     1621 catgattaac cgggtccagt tcgctggaac gctgggcaag catggactgg gtgataacca
     1681 gctcgccgac gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg
     1741 attcactgct gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg
     1801 ccagtttaag cacaggtggg gtagctattt ttggcgaaac ttctactgtt tcaaaggtaa
     1861 tctgatcggc ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatatcgc
     1921 ccggtaatat tgccgagaga gaatccgccc ctttcaccac gtcatttaac gttgtcagat
     1981 gccccagctc ttcttccagc aggtcgactt cgccggcctt caggcgcgaa aggataattc
     2041 gtcgcggcaa ctgactgcga ctttgctcat cttgcggttc acttttggca accacactta
     2101 atcgggtcac tgcggatggc gtttcgcctt tcgcttctaa tgccagttga cgcaaggcct
     2161 ggcagatata atcgtagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct
     2221 gttcttgcat gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca
     2281 tctcacctcg tctggcttca tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa
     2341 cgctgaagcc aaaagttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat
     2401 tcaattgttc ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatatcag
     2461 ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tcgatgctca
     2521 cgctgtcacc tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca
     2581 ggtttttcta gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc
     2641 tgttcggctt gtttgttcag taccaacagg ctgatgcgac ggttgacggc atcatcaggt
     2701 ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcta
     2761 tccaaccctc cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc agaaagctcc
     2821 cagttgctgt aacctttctc accgctggcg tagggaaaat catcggtatg acctgaaagg
     2881 ctaatacggt tgggaatacc gttcagcaca ggcgcgatgg cgcgcagaat gtcgcgcata
     2941 tagggttcga catcggcact gccggttcta aacatcgggc gattctggct atcgatgatc
     3001 tgaatacgta gaccttcctg gaccagatcg attttgagat ggagacgtaa cgcccgcagt
     3061 ttcggatcgg actctatcaa ctggtcgaga tcaccccgca atttccgcaa tcgactttgc
     3121 tccatgcgtt ttttcagctc ttcgatgttc ggctgcttat tcacttcccc ctggctctgg
     3181 gtgtaatcat caccaccgcc gggaattggg ctttcactat tggaaatgcg atcgccgccc
     3241 gtaaccgctg tcgccagtgg agtccggaag tactccgcaa tctgaatcaa ctcttttggg
     3301 ctggagatgg agatcagcca catcaccaaa aaaaaggcca tcatcgcagt cataaagtcg
     3361 gcataagcaa tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg
     3421 acgacaataa tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg
     3481 gatttttcac cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat
     3541 agagcgtttt gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagaaa
     3601 gcagagtgac tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa
     3661 cactcgctag tggggaaata aatccgtaag ccaataaaat accgaggaaa gtccccacca
     3721 tcgcatgtgc gataagcacc cccagctcgg cggcaggacg atcggctgaa cctaacgcgt
     3781 gaacgccccc cattacagcc gcgacaatac caaacgccgg aagtgagtcc ccgaccagcg
     3841 ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca
     3901 gggcttcgat ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga
     3961 taaaatcaag catgacgcta tccgcgagga tgcgtgggta gctggcgaag atctcgctct
     4021 cacggggatt ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca
     4081 tcaaccgata aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac
     4141 gaaacagcaa cggcagcgct ttcagcgtgc ctttaatcgc tttgc
//
LOCUS       DAJTUN010000202         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTUN010000202 DAJTUN010000000
VERSION     DAJTUN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.205"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO470_003791"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO470_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6252883.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO470_003792"
     tRNA            complement(324..399)
                     /locus_tag="OO470_003792"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO470_003793"
     tRNA            complement(406..480)
                     /locus_tag="OO470_003793"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO470_003794"
     tRNA            complement(597..681)
                     /locus_tag="OO470_003794"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO470_003795"
     tRNA            complement(690..765)
                     /locus_tag="OO470_003795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO470_003796"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO470_003796"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6252884.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO470_003797"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO470_003797"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6252885.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO470_003798"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO470_003798"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6252886.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1039 c          929 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAJTUN010000203         4180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTUN010000203 DAJTUN010000000
VERSION     DAJTUN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.206"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            224..2647
                     /gene="pgaA"
                     /locus_tag="OO470_003799"
                     /pseudo
     CDS             224..2647
                     /gene="pgaA"
                     /locus_tag="OO470_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2656..4149
                     /gene="pgaB"
                     /locus_tag="OO470_003800"
     CDS             2656..4149
                     /gene="pgaB"
                     /locus_tag="OO470_003800"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCR6252887.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
BASE COUNT         1173 a          943 c          981 g         1083 t
ORIGIN      
        1 tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaatatctt tcttttcagt tacctgtaat
      181 tagatacaga gagagatttt ggcaatacat ggagtaatac aggatgtatt caagtagcag
      241 aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat ttttagcagc
      301 gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta ttgaagctcg
      361 caagggtaat actcagccag ctttgtcatg gtttgcacta aaatcagcac tcagcaataa
      421 ccaaattgct gactggttac agattgcctt atgggccggg caagataaac aggttattac
      481 cgtttacaac cgctaccgtc atcagcaatt accagcgcgt ggttatgcag ctgtcgccgt
      541 cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa aggcgctctc
      601 tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg cagatgcggg
      661 tcactatgat actgcgctgg ttaaacttaa gcagcttaac tctggagcac cggacaaagc
      721 caatttactc acagaagcct atatctataa actggcgggg cgtcatcagg atgaattacg
      781 ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa caatatccca cagaatacgt
      841 gcaggcatta cgtaataatc aacttgctgc cgcgattgac gatgccaatt taacgccaga
      901 tattcgcgct gatattcatg ccgaactggt cagactgtcg tttatgccta cgcgcagtga
      961 aagtgaacgt tatgccattg ccaatcgcgc cctcgcccaa tacgctgcat tagaaattct
     1021 gtggcacgat aacccagacc gcactgccca gtaccagcgt attcaggttg atcatcttgg
     1081 cgcgttatta actcgcgatc gttataaaga cgtcatttct cactatcagc gattaaaaaa
     1141 gacggggcaa attattccgc cctaggggca atattgggtt gcatcggctt atctcaaaga
     1201 tcatcagccg aaaaaagcac agtcaataat gaccgagctc ttttatcaca aggagaccat
     1261 tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc tagagagtga
     1321 aaattatccg ggcgcgctaa ctgtcaccca acataccatt aatacttcgc cgcctttcct
     1381 tcggttaatg ggcacgccta cgagcatccc gaatgatacc tagttacagg ggcattcgtt
     1441 tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga cagccagaga
     1501 gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga gtgtgttaca
     1561 agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag tgatcgagcc
     1621 acgtaatatt aatctggagg ttgaacaagc ctagacagca ttaacgttac aagaatggca
     1681 gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc ccggcgttgt
     1741 acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag ctggttcaac
     1801 aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa ccaccatcgt
     1861 ttattcacca ctgctgaagg ataactggcg cggttttgct ggattcggtt atgccgatgg
     1921 acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg agtggcggtc
     1981 acgtaatatc tggctcgagg cggagtacgc tgaacacttt ttcaatcatg agcataaacc
     2041 cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg gttcgcaact
     2101 ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta caggcaacag
     2161 tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg tctcctgggc
     2221 tttcactgat ttttccgaca gtaaccagcg tcatgaagtc tcaattgagg gtcaggaacg
     2281 catctggtct tcaccatatt tgattgtcga tttcctaccc agtctgtatt acgaacaaaa
     2341 tacagaacac gataccccat actacaaccc tataaaaacg ttcgatattg ttccggcatt
     2401 tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa tcttcagcgc
     2461 aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc aactcggcta
     2521 cgggcaacgc attagctgga atgacgtgat tgatgctggc gcaacgctac gctgggaaaa
     2581 acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata tgacattcag
     2641 attttaagga taaatatgtt acgtaatgga aataaatatc tcctgatgct ggtgagtata
     2701 attatgctca ccgcgtgcat cagccagtca agaacatcat ttataccgcc acaggatcgc
     2761 gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat ttcatggcat
     2821 aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc agcactgcgt
     2881 gaacaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc tcaaattcgt
     2941 gaagcacatc gaggaggaaa accgctaccg gaaaaagcag tagtgctgac ttttgatgac
     3001 ggctaccaga gtttttatac ccgcgtcttc ccaattcttc aggccttcca gtggcctgct
     3061 gtatgggccc ccgtcggcag ttgggtcgat acgccagtgg atgaacaagt aaaatttggc
     3121 gatgagatgg tcgatcgaga atattttgcc acgtggcaac aagtgcgaga agttgcgcgt
     3181 tcccggctcg ttgaggtcgc ttctcataca tggaattctc actacggtat tcaggctaat
     3241 tccaccggca gcttaatgcc tgtatatgta aatcgtgcat attttactga ccacgcacgg
     3301 tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa aatgacggaa
     3361 tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta tggcgaagcg
     3421 aatggcatag cgatagagga attaaaaaaa ctcggttatg acatgttttt cacccttgaa
     3481 tcaggtttgg caaatgcgtc acaattggat tccattccgc gagtattaat cgccaataat
     3541 ccctcattaa aagagtttgc ccagaaaatt attaccgtac aggaaaaatc accacaacgg
     3601 ataatgcata tcgatcttga ttacgtttat gacgaaaacc gccagcaaat ggatcgcaat
     3661 attgatgtgc taattcagcg ggtgaaagat atgcaaatat caaccgtgta tttgcaggca
     3721 tttgctgatc ccgatggtga tgggctggtc aaagaggtct ggtttccaaa tcatttgcta
     3781 ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tacgtacccg ctcaggtgta
     3841 aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt aacgcgagta
     3901 aaatacttac caacagggga gaaaaaagca caaattcatc ctgaacaata tcaccgtctc
     3961 tctcctttcg atgacagagt cagagcacaa gttggcatgt tatatgaaga tcttgccgga
     4021 catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga ttatgaagat
     4081 gccagtgcac cggctatcac ggcttatcag caagcaggct ttagcgggag tctgagcgaa
     4141 attcgataaa acccggagca atttaaacag tgggcccgct
//
LOCUS       DAJTUN010000204         4174 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTUN010000204 DAJTUN010000000
VERSION     DAJTUN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.207"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(1..2461)
                     /gene="narZ"
                     /locus_tag="OO470_003801"
                     /pseudo
     CDS             complement(1..2461)
                     /gene="narZ"
                     /locus_tag="OO470_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(2543..3931)
                     /gene="narU"
                     /locus_tag="OO470_003802"
     CDS             complement(2543..3931)
                     /gene="narU"
                     /locus_tag="OO470_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6252888.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINDGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1058 a         1061 c         1110 g          945 t
ORIGIN      
        1 tgcgtgattc ccaggccgga tcgaccgccg cagaaagcgg atgaataaat ggatgcatat
       61 ccgaggtgtt catatcgtct ttttcatacc aggtggcggt gggcagaacg atatcggaga
      121 acaggcaggt actggacatg cggaagtcga gcgtcaccag caggtcgagc ttgccttcaa
      181 tcgccgcagt ttgccactcg acttcttccg gtttgatccc gtcgctggca ccgagttcct
      241 cgccctgaat cccgctttcg gtccccagca gatacttctg catatactcg tggcctttgc
      301 cggaggagcc aagcaggtta gagcgccaga caaacaaatt acgcggatga ttgctgccgc
      361 tatctggttg ttcgcaggcc atacgtaaat cgcccgattt cagcgcctgg gcggtaaatt
      421 ctgtggggga taatccggcc ttgtcggctt cagctttaat cccgagcggg ttacgcccca
      481 gctgcggcgc agaaggtagc cagcccatac gttcggcgcg aacgttgaaa tcaatcagat
      541 gaccgctgta cttactggca tcggcgagcg gtgaaagtaa ctcctgagca gagacttttt
      601 catagcgcca ttggctggaa tgattgtaga aaaacgaggt gctgttcatc tggcgcggtg
      661 gtcggttcca gtcgagcgca aaggccagtg gcaaccagcc ggtttgtggg cgcagttttt
      721 cctggccgac atagtgtgcc cagccgccac cgctttgccc gacacaaccg cagaagatca
      781 gcatattgat catcccgcgg tagttcatgt ccatgtgata ccagtggtta acacctgcgc
      841 cgaggataat cattgagcgc ccatgcgttt tatgggcggt atcggcaaat tcgcgggcga
      901 tggtttcaat atactggcgc ggtacgccgg taatttgctc accccaggct ggggtgtacg
      961 gtttgatttc agcgtaatct ttcgcgctgt tttcatcttc cagcccgcga tcgaggccgt
     1021 aattcgccag taccaaatca taaacgctga ccaccggaca ggtgttgcca tcgactaacg
     1081 tcaggttttt aacgggcaat tggcgcacca gcaccggatt gtgttttacg ctgcgaaaat
     1141 gcggattttc aatgccgcca aagtagggga aggccacgcc tgcaacagcg tcatgttgac
     1201 cgagcagggt taacgacaat tcggtttccg taccggcggc aatggattcc agattccatt
     1261 tgcctttttc tccccagcgg aaaccaatcg aaccgttcgg cactaccaat tcacctgcgg
     1321 tattaactgc tacggttttc cactgcggat tgttgctttc gcccagtcca tccaccaggt
     1381 cagatgcgcg gatcatgcgc ccgggaacgt agctaccatc gtcgcgaggc tccagcatta
     1441 ccagcatcgg catgtcgctg tagcggcggc agtagttgat aaagtagtcg ctgggattat
     1501 cgagatgaaa ctcttttaaa atcacatgcc ccattgccat cgccagggcg ctatcagtgc
     1561 cttgtttcgg tgccagccac tggtcgcaca atttggccac ttcagagtag tcaggggtaa
     1621 tggcgatggt tttagtgcct ttgtagcgta cttcggtaaa gaagtgggcg tccggcgtac
     1681 gtgtctgcgg tacgttagac ccccaagcga tgatgtagct ggagttatac cagtcggcag
     1741 attccggtac gtcggtttgc tcgccccagg tcatcggcga ggcgggcggc aggtcgcaat
     1801 accagtcgta gaaactttaa caggtgccgc caagcagcga cagataacgc gttccggcgg
     1861 cgtaagaaac catcgacatc gccgggatcg gcgagaaacc ggcaacgcga tccgggccgt
     1921 aggttttgat ggtccagacg ttagcggcgg caatcagctg gtttagttcc tgccagttgg
     1981 agcggataaa cccgccgcgc ccacgcactt gtttgtagct caggcacttt tgcggatcgt
     2041 tcataatcga tgcccacgcc agtaccggat cgctgtgttg tttgagggct tcgcgccaca
     2101 gttcaatcag tcgtttacga atgagcgggt atttcaggcg gttagcgctg taaagatacc
     2161 aggagtaact tgcgccacgc gggcagccgc gaggttcatg attgggcagg tcagggcgag
     2221 tgcgcgggta gtcggtctgt tggatttccc aggtcaccag accattttta acgtagattt
     2281 tccagctaca ggagcctgta cagttaacgc cgtgggtgga acgcacgatt ttgtcgaact
     2341 gccaacgctg gcgatagctg tcctcccagt cgcggttgct atgcatcacc tgtccgtgac
     2401 catcggcaaa ggtttcgccc ttttgtttga agtagcgaaa gcgatccaaa agtttactca
     2461 tgacatgact cctgctccag gaatgaattg ggatctgatg ccttgtcgga tgcgacatgg
     2521 ttgtacatca ggcatcgatc tcttattttt ggctgaactt ccgccgacca taaaccagcc
     2581 aggtcagcag cacacaaacg atgtagaaga ttaaaaacac tttcatcgcg ccgaccggag
     2641 agccggtcat attgagcgac atgccaaatg cctgcggaat aaaaaagccg cccactgcgc
     2701 caatggctga gataaagccc agcgttgccg ccgtttcggt gacggcttct ctctgagctt
     2761 gctcatcact accgcctttc atcttcaccc gataaatggt tatctggcga aagatgacgg
     2821 cgatcatctg gaaagtagaa ccacttccca gacccgcggt cagaaacagc cccataaata
     2881 cggcgtaaaa ggcgatgaaa ttaccggagc ctgtgcccgg taaggtaagg aacagcaggg
     2941 cactgaaaat cgccataaaa ataaagttga tcaacgtcac ccgcacgccg ccgaatttat
     3001 cggaaatagc accaccaacc gaccgcgcga tggcaccaat aaatgggcca aagaacgcca
     3061 ggcgcagaat attcacatcc gggaactggg tttttgccag catggcaaaa cccgcagaaa
     3121 aaccgataaa cgaaccgaag gtggcaaggt aaagcaggct cagcagccag agatgcaggc
     3181 gttgtaagac agggagctgg tcggcaattg aggcgcgtga actggcgata tcattcatcc
     3241 ctgaccaggc ggcgatcgtg gcaatcgcca gtagcggtac ccaaatccat gcggcattcg
     3301 ccagcgacat caccgaaccg tcggcctgcg gtacgccatt gacgccgaga aaggcaaata
     3361 caggtacaaa aatgaccagc ggtgcaacca gctgcattac acttacacct aagtttccta
     3421 atccgtcatt aatcccaaga gcgctccctt gtttggcttt tggaaagaag aaactgatat
     3481 tgcccatgct cgaagcaaag tttgcacctg caaaaccgca tagcaaagcg ataacgataa
     3541 atatcccaaa aggagtattc ggattttgca cggcaattcc gagccagacg caaggaataa
     3601 tcaggattgc agtactaaaa accggccatc ggcgtccacc gaatataggc accataaagg
     3661 agtagggaac acgcaataat gcgccagaaa cggagggtaa tgcggttaat aaaaagagtt
     3721 gatcggtagt aaaattaaaa ccgattttat tgagattaac ggtaactgcg ctaaatagca
     3781 tccagacaca gaaggcaaga agtagacaac tgactgatat ccagaggttt cttcgagcaa
     3841 tatgttttcc tttattttcc cagaaggccg gattttctgg tttccagtcg cgtaaaagat
     3901 aacgactatt tttctcattt tgcagtgcca tattgttcct catatgcaca cattggtaat
     3961 gaaaaaagac aaaacaggag gtaaggcgca atagccagtt attagaatta aggatgaatt
     4021 gggtgaagtg ctgattgaaa gaatagataa gaaagcgtaa ccgcgggggc agaatgggga
     4081 ttaagtagcc agatatgtgt tactaaatgt aactaacaag ctaaccttca aacgggggtt
     4141 aatctttgaa cgtatgtcat gtttcaaccc ttca
//
LOCUS       DAJTUN010000205         4158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTUN010000205 DAJTUN010000000
VERSION     DAJTUN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.208"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..816)
                     /locus_tag="OO470_003803"
     CDS             complement(<1..816)
                     /locus_tag="OO470_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6252889.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRA"
     gene            complement(813..1949)
                     /locus_tag="OO470_003804"
     CDS             complement(813..1949)
                     /locus_tag="OO470_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6252890.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(1942..>4158)
                     /locus_tag="OO470_003805"
     CDS             complement(1942..>4158)
                     /locus_tag="OO470_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252891.1"
                     /translation="SLIESQRPRYVLIEGPADFNDRVDELFLAHQLPVAIYSYCQYQD
                     GAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDLPCWAQSEEEDDSPDTQEESQT
                     LLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFAQLRGDSPGDALNRQREAFMAR
                     WIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVINTPELPSLADAVTGCYLTPYSE
                     KRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKTVLTRLRQHKLPASTADMAAAH
                     LHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPLPWSYRGVIHPDTDPILLTLID
                     TLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAELTLNRFNPNGLAQSQVLHRLA
                     ILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAALIEAACFGATLQEAARHKLEAD
                     MLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLIAQENQFAEMGQALEVLYALWR
                     LDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEKEFHAHLHSWQALCHILRDLHS
                     GVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGALMRLEHPNASAEAALTMLAQLS
                     PAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLSDADFTNALPDLRAAMAWLPPR
                     ERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHHQQLEQQALASLQHWGVFHV"
BASE COUNT          919 a         1147 c         1128 g          964 t
ORIGIN      
        1 ggcgcgcagt tgccgtagcg attcactcac ccatcgccag ctgcagcgtt ccgctgcttg
       61 ctgcgcgcgg ctgaaagcgg cctcgtagtg aataagcggc tggctgatgc cgctcagcca
      121 taatgcctgg cttagttgtt gaacatattg acgacacgtt ttgccttctt cgctggcaaa
      181 cggatcgtca gatgatgtga cgtgttcgct gcgcatctgc cagattaaat gagtcaattc
      241 cgcttgctgc gcttttgcct ggacaaaggc ctgtactgcc agtacgacat gttcgcaaag
      301 tgtgccttca atacaatcac aacgggcgaa acgaatactg ctgcgggaat aaaaacgcac
      361 atcgctcatc ggtaagcggg cagagggaat ttcacccggc gcacagaaca actcaatggt
      421 gatgctttta gcgaccagcg cctgtgcgcg tttgcgggtg gcatcgggaa gggtagccag
      481 ttcttccagc cagattgccg gatcccattc ctcttctttt cccgtaggct gggcggtagc
      541 acaaagtcgt tgataactta acaccagcat cacgcgatga cggcacatgc cgctggcccc
      601 ataggtgcac tgagcctctt tcagtgcctg gccgttcgcc agctgggtac ggacaccgtc
      661 actgaaggtg gcgattaaag cgctgttctc atggctgatt tccgggacgt tgccattttc
      721 cagttcctta aggctgcgct taacaaaacc ggcattgctt aacgccgtca gtgcctgcgg
      781 tgtcagttct aataattccg gacgtagtga attcatgact gaagattctc cgcaagccat
      841 gatgccagct cgcccggtgt catggcggct atttgtgcgc cgacattaat cagcgcctgg
      901 gccgtatcgc ggtcatagca aggtgttgct gtgctatcga gcgctgccag tcccagcact
      961 ttgatgccgc tctggacaca ctttttcact tggtgcgtca gcaatgatga tgaaccccct
     1021 tcataaaaat cgctcacgag gataatgacg ctttttgcag gttgttcaat aagttgccga
     1081 ccatactcca tggcactggc gatattggtt ccgccgccta attgcacttt cattaataac
     1141 tctaccggat cggcaacgtc tgccgtgaga tcaacgacgc ttgtgtcaaa cgccaccaga
     1201 tgggtacgaa tgccgggtaa ctgccataaa caggccgcca tcaccgcaga gtggatcacc
     1261 gaatcgacca tcgatccgct ttgatcaacc agtaagacca gttgccattg ctcgctttgg
     1321 cgtttaatgc ggctgttaaa gcgggggaat tcgatataca acttgccgtg ttgcgggtgc
     1381 cagtgttgca ggttggcgcg cagagtactt ttgaaatcaa agtttcgcgc cagtggaata
     1441 aatgagcggc gacgacgatc gcggacacca gaaaaagcct gacgaacttc ctttgccagt
     1501 cgcgccataa tttcttccac aacctggcgc acaatcctgc gggcggcagc cagtacttcg
     1561 gggttcatca gatgtttggt gtgcaaaacg gcgcgtagca ggctttcgga aggctgcata
     1621 cgttccagca cgtcgagatt tgtcaccaca tcttcaatgc cgtagcgcag tacggcatcg
     1681 ctttccagcc gctcaatcac ctgttgcgga aacagcgtgt gaatactgtt gatccactca
     1741 ggagtggtga gatttgagcc acctaatcca ctagagcgtt caccacgctg gagccgttca
     1801 ggatcgcgcc catacagcca ctccagcgcg tgatctatct gccgggcgtt gtcatccagc
     1861 ccacaaagcg tcgtttctgc cgcttcgcca agaattaatc gccagcgttg tagctcacgg
     1921 gtggtcagaa gatcgttcag ttcagacatg gaaaactccc cagtgttgca gcgatgccag
     1981 tgcctgctgt tcgagttgtt gatgatgtgc aatggcttgc ggtggacaat gcaacggcat
     2041 ttgcagtgcc gaaacgggaa gttgcgccag ttgataatgc tcaagcacct gatgcgccag
     2101 cgtcccgcgt tctcgtggcg gtagccaggc cattgccgcg cgtaaatcgg gcagggcatt
     2161 ggtaaaatcg gcatcactta gttgatttaa atgactgctg aaaccggcga taaatgccgg
     2221 ctgacaggcc agttgatgac gggctaatgc cagcaaaccg tgcagcgcct cgccggattg
     2281 tgccggggat aactgcgcca gcatcgtcag cgccgcttcg gcactagcgt tgggatgttc
     2341 cagacgcatc agcgcaccaa gagtcgcgcc gcgatccagt gccaggacat gaatcgcctg
     2401 actgtgtcgc tccagcaagg ctaccgccgc agaaagcgaa acgccgggga gttgcacacc
     2461 gctatgtaga tcgcgcagga tatgacacaa cgcttgccag ctatgcaagt gagcgtgaaa
     2521 ctccttttca tccggtctgc cgttggattc acacagccac agcgtgcgat cgatagccgc
     2581 gcataacgtc gtttgtaata tctgcgcgcc ttgcataccg ctaatttcat ccagccgcca
     2641 taaggcatat agcacttcca gcgcctggcc catttcggca aattgatttt cctgggcgat
     2701 taataatgtg agttgctcca gtaattgttg actgaaggac gccagacccg ctgacgccgc
     2761 ttggctaaga catgtggtaa tgctgccgat tccacccgcg tccagcatat cggcttctaa
     2821 tttatggcgt gcggcttcct ggagtgtggc accaaagcag gcggcctcaa tcaatgccgc
     2881 atgctggcta agcgggcggg ttaatttcca gcgttcttca ccgttgcctg caagtgtcag
     2941 tgtgcttccc tgctggcgta caatccctgg gatctccagt attgccagcc gatgtaacac
     3001 ctgactttgc gctagtccat tggggttaaa gcgatttaag gtaagctccg ccggaagaga
     3061 gattgcggta cgttccagtt cgcaagtgac atcttttggc agaggcggtt gtggcgtaga
     3121 aggggcaagt ttaccgaatc cgtcacccgc taatgtgtct atcaacgtta gcagaatcgg
     3181 atcggtatcg ggatgaataa cgccgcgata gctccacggc aacggcgcat tcagggcttc
     3241 tttaatcagc gatcctgcta tggcatccag ccagtcagtg cgtaacggta gtgtatgacc
     3301 gcgcaactgt gccagtgcca tcgcatgcag atgagcggca gccatatccg cggtcgaagc
     3361 aggcaacttg tgctggcgca aacgggtgag aaccgttttt agcagttgtt ctcccgcctg
     3421 ctgtaagccc cactgccagc accagttttg ccagaccggg gcaggcattc ctgaaaggta
     3481 tcctgccagc acatcaaggc gcttttcact gtagggggtg agataacaac ctgtaacggc
     3541 atctgccagc gacggcaatt ctggcgtgtt aattacctgc ggacattcgc gccacatttt
     3601 tgccagtgcc ggagcgtgcc agccaccgca gacaactaac acgtcgccat tattttgctg
     3661 catcgcccat ccaatccagc gggccataaa ggcttcgcgc tgacgattta gagcatcgcc
     3721 gggggagtcg ccccgcagtt gggcaaaata gtgcgccagt gcagagggta atgcagtttg
     3781 ctggctttca tcttcgaaca agtgatccca cagggtgtcg ctgttatcca tgcgggtggc
     3841 acgcagcagc aaggtctggc tttcctcttg cgtatcaggc gaatcgtcct cttcttcact
     3901 ctgcgcccag caaggcaaat cgatgaagta agtttgtgcc tgaatacgac gatcggcttg
     3961 tagcgcctgc cactccggcg aaaattcagc aaatggcgtc caggcaccac gcccgggggc
     4021 tgcaccgtcc tgatactggc aataactgta aatagctacc ggaagctggt gggctaaaaa
     4081 cagttcgtct acccggtcat taaaatcagc agggccttca atcaacacgt atcgtggccg
     4141 ctggctttcg attaacga
//
LOCUS       DAJTUN010000206         4153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTUN010000206 DAJTUN010000000
VERSION     DAJTUN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.209"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            144..2368
                     /gene="ybiO"
                     /locus_tag="OO470_003806"
                     /pseudo
     CDS             144..2368
                     /gene="ybiO"
                     /locus_tag="OO470_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(2365..3291)
                     /gene="rlmF"
                     /locus_tag="OO470_003807"
     CDS             complement(2365..3291)
                     /gene="rlmF"
                     /locus_tag="OO470_003807"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6252892.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3567..3827
                     /locus_tag="OO470_003808"
     CDS             3567..3827
                     /locus_tag="OO470_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6252893.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
BASE COUNT          961 a         1108 c         1032 g         1052 t
ORIGIN      
        1 cccgcacgtt gtcagcaacc tgcttaatat cccttcctcc ctttcacccg aaagggaggc
       61 acaccagatt cctctcattt aaaatcgccc ctcccccagc atctatactt atctttttgc
      121 tctgttttct cactggagga gtcatgcggt ggatcctgtt catcctcttc tgcctgctgg
      181 gcgcacctgc ccacgcggta tccatacccg gcgttacaac cacaacgaca acggactcaa
      241 cgactgaacc ggccccggaa ccggatatcg aacaaaaaaa gcggcctatg gcgcactggc
      301 ggatgtgctg gataacgaca cctcgcgtaa agagttgatc gaccagttgc gtactgtcgc
      361 cgcgacgccc cctgcggaac cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca
      421 aaccgtgctg caaaaggtca ccgaagtcag ccgccattat ggtgaagccc tttccgcccg
      481 cttcgggcaa ctttatcgca atatcaccgg ctccccgcat aagccattta atccacaaac
      541 cttcagcaat gcgctgagcc atttttcaat gttagcggta ttagtgtttg gtttttactg
      601 gctgattcgc ctgtgcgcac tgccgctata tcgcaaaatg ggtcagtggg cgaggcaaaa
      661 aaatcgtgag cgcagcaact ggttgcagct tccggcgatg attattgggg cgtttattat
      721 cgacctgctg ttactggccc tgacattatt tgtcggccag gtattaagcg acaacctgaa
      781 tgcaggcagt cgcaccatcg ctttccaaca aagtttgttt ctcaacgcct ttgccctgat
      841 tgaatttttc aaagccgtac tacgcctgat tttttgccca aacgtggcgg agctgcgccc
      901 gttcacgatt caggacgaga gcgcccgtta ctggggtcgt cgcctgagct ggttaagcag
      961 cctgattggc tatggcctga ttgtggccgt gccgattatc tctaatcagg tgaatgtaca
     1021 gataggtgcg ctggcgaacg tcatcattat gctgtgcatg accgtctggg cgttgtacct
     1081 gatcttccga aataaaaaag agattaccca gcatatgctc aactttgcgg agcattcgct
     1141 ggcttttttc agcctgttta tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc
     1201 ctattttatc atgctgtttt tcttttcgtt gttcgatccg ggcaacagtc tgaaatttat
     1261 gatgggtgca acggtgcgca gcctggcgat tattggtatc gcagcgtttg tttccggtat
     1321 gttttcccgc tggctggcga aaaccatcac cctctcgcca catactcagc gtaactatcc
     1381 ggagctacaa aaacggctga atggctggct ttcggcggcg ctgaaaacgg cgcgtattct
     1441 gaccgtctgc gtggcagtaa tgttgctgtt gagcgcatgg ggattgttcg atttctggaa
     1501 ctggctgcaa aacggcgcgg ggcagaaaac cgtagatatc ctgatccgca tcgcactcat
     1561 tcttttcttc tcggcggttg gctggacggt gctcgccagt ttgatcgaaa accggctggc
     1621 ttcggatatt catggccgcc cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt
     1681 ccgtaacgcg ctggcggtga ttatcagtac catcaccatc atgattgtgt tgtcggaaat
     1741 cggcgtcaat atcgcgccat tgctggcagg tgccggggca ttaggtctgg ctatctcgtt
     1801 tggttcgcaa acgctggtaa aagatattat caccggggta tttattcagt ttgaaaacgg
     1861 catgaacact ggagatttgg tgactatcgg gccgttgacc ggcacagtgg aacggatgtc
     1921 gattcgctcc gtgggcgtgc gccaggatac cggggcgtat cacatcattc cgtggtcttc
     1981 gataaccacc tttgctaact tcgtccgcgg tattggttcg gtagtggcaa actacgatgt
     2041 tgatcgccat gaagatgctg ataaagccaa tcaggcactg aaagatgcgg taacggaatt
     2101 gatggaaaac gaagaaattc gcgggctgat tattggtgaa ccgaactttg ccgggattgt
     2161 cggcttaagc aataccgcgt ttacactgca tgtttcgttc accacgctgc cactcaaaca
     2221 gtggacggta cgctttgccc tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt
     2281 tcgcgcgcca gtgcagactt atcaggtgct gcctgctccg ggcgcgaccc cggctgaacc
     2341 gctgccgccg ggggagccaa cgctttaacg ttggcgatta acaaaacggc ggcgctgctc
     2401 gtcgttcata aaggtccagg caataaagcg actctgcttt tgcccctggg ccatctcttt
     2461 ttttaccacc ttcaccgcgc ccacgtcggt cagagcacga tacaacggcg gtaagttttc
     2521 accacgagat accagcgagg taaaccacat cacctgcttc gcgaagcctt tgctctcttc
     2581 aatcatcttt ttgataaagt tgacttcacc gccttcacac cacaactcct gttgctgacc
     2641 accaaagttc agtgcatcgt ctttgttcag ccccaggtta cggcgtttac gctcactacc
     2701 tgcccgcgcc gcagcggcgg aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg
     2761 ctcgtttttg tggatgatgc cgttaaaaat cgccccactc tctttttgcc gacgcagacg
     2821 aatggcgcgg ttaagccccg gattagcact gataatcgcc tgcgcactgc ttaacgcctg
     2881 gctgctggtt tcactaccgg taaaacgcca gccatattca tgtacgccaa tcagcggata
     2941 aatacagttc gcaccaacgc cgatatccag aatgctggca ttcgctggaa ttgttccgct
     3001 tgcttcagcc agtaaatcgg caaggtgatg aatataatcc gcccggcccg gtactggtgg
     3061 gcagagaaaa ccgtcgggga tatcccagtt cgctacggcg taaaaatggg ccagcaacgc
     3121 cttattgagc gccttcaccg ccagcggatt ggcaaagtct acgctttgct cccctgcggg
     3181 tgtaagcgtg aggaattgcc tgagttcagg attgacctga caaagcgtgg cgagatcgta
     3241 gcggctgtga tgacggttgc gcggatgcaa ccccggtttc tgggcggaca tagcattctc
     3301 ctttaagcag cggcgtaaga tacccgcata acgcgacagg gtaaatatct ggctgcgttt
     3361 atcattcccc aaaaccttaa catggctatg gtttatggca attcatcata aaaatcagca
     3421 ttatgcatgt cattgatttg tcttattttg taaaaatata tcgttcaaaa aataagcata
     3481 tctctgtcaa ttttttgcgt catttgcatc gcgcaggcga cctacactta tcaggcacta
     3541 cccgttgtat ttatgaggat gtcattatga aaaagtgcct cacactactg attgccaccg
     3601 tcctgagcgg aatctctctc acggcttatg ccgcgcaacc gatgagtaac ctggacagcg
     3661 gtcagctacg gcccgccggt accgtttcgg caactggcgc atcaaaccta agcgatctgg
     3721 aggataaact ggcagaaaaa gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg
     3781 caggcggaaa tgaccagatg ttaggtactg caaccatcta caaataaccc caccaacgta
     3841 actgcattcg cctctggatg cagttttcgc ctgttttgta atcaaaaaac atcaaatatg
     3901 aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg gtgatagaaa atcgcatcaa
     3961 gtgataatgc ttatcaaaat tactatcact tgcacgaaca ctatcacggg attaacagtg
     4021 gcatcgcatc cgcagagatg ctttctcgtg gcagtgaaaa tttcaacata taagaaaaag
     4081 tcacctgcaa aatggaaaac aatcgcaatt tccctgccag acaatttcat tcgctcacgt
     4141 tctttgccgg tct
//
LOCUS       DAJTUN010000207         4136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTUN010000207 DAJTUN010000000
VERSION     DAJTUN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.210"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..431
                     /locus_tag="OO470_003809"
     CDS             <1..431
                     /locus_tag="OO470_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR6252894.1"
                     /translation="ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLY
                     FIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMS
                     LCILIFLALMNKVSENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            374..763
                     /locus_tag="OO470_003810"
                     /pseudo
     CDS             374..763
                     /locus_tag="OO470_003810"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            743..1300
                     /locus_tag="OO470_003811"
                     /pseudo
     CDS             743..1300
                     /locus_tag="OO470_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1398..3007
                     /locus_tag="OO470_003812"
                     /pseudo
     CDS             1398..3007
                     /locus_tag="OO470_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3108..3599)
                     /locus_tag="OO470_003813"
     CDS             complement(3108..3599)
                     /locus_tag="OO470_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HCR6252895.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(3604..3780)
                     /locus_tag="OO470_003814"
     CDS             complement(3604..3780)
                     /locus_tag="OO470_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6252896.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1260 a          668 c          828 g         1380 t
ORIGIN      
        1 ttgctttggt tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac
       61 gatcaaccta tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt
      121 ttatgcagtt gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg
      181 cttttctgag ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg
      241 aggggcgttt gacaagcagt gttgtccaaa atgacattgc aatctattat ttgtttcgtc
      301 agatgagttt gtgcatatta atatttctgg cattgatgaa taaagttagt gaaaacacaa
      361 aacagcgcaa tttattttcc aaaaaaatga ctttgtgcat aagtttgttt ttgtttttgg
      421 ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt tacacattgc
      481 cgagttaacc aatgaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      541 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctatgatat
      601 ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct ctctgttatt
      661 tatgagcagg tatagcgtgt ccacttggta tattagtcgc actattgaag tagtcagtaa
      721 gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag taacaaagaa
      781 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
      841 gacagttcaa tcagcatcag ccaaaaaaac gccttattgt gtcatgatta tggatatcga
      901 ccacttcaaa gaagtcaacg acacctgagg gcatccggtt ggcgatcagg tgataaaaac
      961 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgtcggcgg
     1021 cgaagagttt ggcgtcttgc tgacggacat cgatactgaa cgtgcgaaag ctttagcgga
     1081 aaggattcgg gaaaatgttg agcgtttaac tggcgataat cctgaatacg ctataccaca
     1141 aaaagtgacg attagtattg gcgctgttgt gactcaggaa aatgcgttaa acccgaatga
     1201 gatctatcga ctggctgata acgcactata cgaggcgaaa gagaccgggc gtaataaggt
     1261 ggttgtgagg gatgtggtga gtttttgtga gtcaccataa agcggcattt tagcgggtga
     1321 ttttggtaag cgctaaatag ctattcgcag acttgcgtaa caattttcag aaatatcata
     1381 gtactatatg atcttaaatg cttatcattg atcaatctgc tatcacccgc ttttactggc
     1441 aattgtacta taatttctta ggttgcatca ccctgatgcg tggggaaaca tgtatggtat
     1501 tcaacaaaaa aatgtttgta ctcattataa taccaggcat tctgggggtg ttactatcgt
     1561 ttgctatgtc aatatttcaa atgaatagag atacgacaat taccgccggt attctgttaa
     1621 aacagcttga taatgttacg caaattgtcc agcatacgac aaagcttacc tctattctgg
     1681 tgatgaagcc ctgcaaagac attcttgaac agttgattgc taacggagca ttgactccct
     1741 atgttcgaac aacgggtctt atagaaaata atttccaaat atgttcttca gttagcggtt
     1801 tcaaaaaaat gaatgttaac gatgtttacg gtacgagctt tcacaataaa aataaagaat
     1861 cacgtatagt atcaattagc ggtacaagtt ttgtgccagg taaaacagca atagtgttcc
     1921 ttatgccaat agggaatgat atgacagctt ttagtattgt agagtcacga tatatctatg
     1981 atctaatgga tgttcttgat gatgagaatg atgattcatt ttctcttcgt tttactgaag
     2041 ggcctgcaat tattagcggt gtaaataata atgacaggtt atatatgtta aaaagagatt
     2101 ttaattctgc gatcagtcag gctcgtctta cagttactac gccgatgata tcgttatatc
     2161 cctatatcat tagcaatgtg ctttatattt tgccgttatc tattattctc tcttttattc
     2221 tctatttctt atggcagcac tggatgtcac ggaaaatgtc gctggcagaa gaaattaaaa
     2281 aaggaatgtc atccggagaa ttttctgtgc attatcaacc agtgtgcgac acgacaacca
     2341 aagcgtgtct gggggttgaa gcgttaatgc gctggcaacg cgaagatggt aaaaatatat
     2401 ctccggtagt ttttatccgc gctgcagaag aggaaaattt aataataccg ttaactaaac
     2461 atctctttga actgattatt caggatgtac aaagctggaa agtgaaaaag ccgtttcacc
     2521 ttgggataaa tattgccgcg gaacatcttg cacatcctga ctttgttggt gatgttttac
     2581 atattaaaaa tgcaatatct gataaattta atattgtcct tgagattacc gaacgcaatc
     2641 tggttgaaga cactgatctt gcattacaga aaataaatga attaaggatc catggctgtg
     2701 aatttgcagt cgatgatttt ggcacgggat attgttccct tggccttctg caaaagttgt
     2761 ctgttgatta tcttaaaata gataaaagct ttatagacac acttactaca gcggaagacg
     2821 aaacgccagt tttagatatt attattaagc tgagtaatcg gctcaatttg ataacgattg
     2881 cagaaggtgt cagtactcct caccagcaga atatcttatt cataataacg tgacattagt
     2941 tcagggatac ttatatgaaa aaccaatgaa agcgacagag ttttatcagt ggtatgtaaa
     3001 cagataaata attaaaaata ttggaatggt tttgtattgt cttaaaatat aaacaaagca
     3061 atcagaatgt tattaactga tgacaaacgc caagatcaac aatgctgtta gtgagtaata
     3121 catatgaatc gtggtgtcct gcctgtagag aatatgtagt tataatcatt ttgacgcgta
     3181 acataataat taattgaagt gtctgctttt atgaagaaat cagaagtgat attttcaagg
     3241 atttcatcgc tgatgttatc atctttttgt attttttgga ttttaactgt ggtgagataa
     3301 acggtatcac ggaatttctt gtatgtaaaa ctcacatatc ggcggatgaa gcccattttt
     3361 tttccatcta ccgtgtatgc gcccgtgaga tacatgtttc ctgtgttatt ttcagaaatc
     3421 atatttaacg aaaaatattg atccattttt gtatgatcgg tgaaattaat aacatgggca
     3481 ttacatgata tgttttgctc tgctggttca ttggaatgat gaaacgcaat atggctcaga
     3541 attgctgcaa taaaagcaca taaaattatt cccgaaaaca gaaaatatct ttgagtcata
     3601 tgattactcc gctttataat agagagagtt gcaattattt ggtgattcag agtttaacat
     3661 ttgactacag gtgaaagcat aaaacccgct catgctatca tttgttgcaa tgtaaatgta
     3721 aggatgtatt ttgcagttga cttccatttt ctgcaatttt tcaataattg gtgatagcat
     3781 gtctacggaa ttaaagtaat tattatcata gaaataactg catccttcct gagtagggtg
     3841 ttgagcataa tttgagaaag catctatttt ttttatagtg tgagatttgc tttgaaaagc
     3901 catatcgaaa aaataagaag acatataaac tatcccccat gccgcaaaaa aaaactataa
     3961 gtaatgtatt ctttgtggta aatatacctt ttgcaacgta atttttattt atattttcag
     4021 tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta ttgtcgagaa
     4081 cagttatgtt gatttcttca ttgattttga agcctttccg tggcaccgtt ctgata
//
LOCUS       DAJTUN010000208         4110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTUN010000208 DAJTUN010000000
VERSION     DAJTUN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.211"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(954..1145)
                     /locus_tag="OO470_003815"
     CDS             complement(954..1145)
                     /locus_tag="OO470_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6252897.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="OO470_003816"
     CDS             1209..1532
                     /locus_tag="OO470_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6252898.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="OO470_003817"
     CDS             1522..1833
                     /locus_tag="OO470_003817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252899.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="OO470_003818"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="OO470_003818"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="OO470_003819"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="OO470_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="OO470_003820"
     CDS             2748..2981
                     /locus_tag="OO470_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6252900.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="OO470_003821"
     CDS             complement(3527..3994)
                     /locus_tag="OO470_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252901.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1001 a          938 c         1194 g          977 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DAJTUN010000209         4091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTUN010000209 DAJTUN010000000
VERSION     DAJTUN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.212"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1648
                     /gene="torZ"
                     /locus_tag="OO470_003822"
     CDS             <1..1648
                     /gene="torZ"
                     /locus_tag="OO470_003822"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6252902.1"
                     /translation="DNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQ
                     FEEYLTGKSDNTPKSAVWTAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQ
                     KHWMLVTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADD
                     GGMTAIPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAW
                     QKPEMIVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFE
                     ARNDFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFW
                     QQNKLIEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHP
                     TWLAPDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAAR
                     FGIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTA
                     DIPSSQRANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(1813..2784)
                     /gene="cmoB"
                     /locus_tag="OO470_003823"
     CDS             complement(1813..2784)
                     /gene="cmoB"
                     /locus_tag="OO470_003823"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6252903.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2781..3524)
                     /gene="cmoA"
                     /locus_tag="OO470_003824"
     CDS             complement(2781..3524)
                     /gene="cmoA"
                     /locus_tag="OO470_003824"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6252904.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3565..3960)
                     /locus_tag="OO470_003825"
     CDS             complement(3565..3960)
                     /locus_tag="OO470_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6252905.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
BASE COUNT         1101 a         1022 c         1071 g          897 t
ORIGIN      
        1 cgataatgcc acctggatcg cgccgaatat gggcaccgac gtggcactga tgttagggat
       61 tgcgcatacc ctgatgacac aaggcaaaca cgataaagtg ttccttgaaa aatacactac
      121 cgggtatccg cagtttgaag agtatctgac aggtaagagc gataatacgc cgaagagcgc
      181 ggtctggacg gcggaaatta ctggcgtacc ggaagcgcag atagtcaaac tcgctgaact
      241 gatggcggcg aatcgcacaa tgctgatggc aggctgggga attcagcgcc agcaatatgg
      301 tgaacagaaa cactggatgc tggtgacgct ggcggcgatg ttagggcaga ttggtacacc
      361 gggcggcggt tttggtttct cttatcatta ctccaatggt ggcaacccga cgcgtgttgg
      421 cggcgtgctg ccggaaatgt ctgcggcgat tgccggacag gccagcgagg ccgctgatga
      481 tggcggaatg acggcgattc ctgttgcccg tatcgtcgat gcgctggaaa atcctggcgg
      541 caaatatcag cataacggta aagaacaaac gtatccgaat atcaaaatga tctggtgggc
      601 gggtggcggt aactttaccc atcaccagga tactaatcgt ctgatcaaag cctggcagaa
      661 accggagatg atcgtcgttt ctgaatgcta ctggaccgca gcagccaaac acgcagatat
      721 cgtattaccg atcaccacat cgtttgagcg caatgacttg acgatgaccg gtgattacag
      781 caaccagcat attgtgccga tgaagcaggc tgttgctccg caatttgaag cgcgtaacga
      841 ttttgacgtg tttgccgatc ttgcggaatt actcaaacct ggcggaaaag agatctatac
      901 cgaaggtaaa gatgaaatgg cgtggctgaa atttttctat gatgccgctc agaaaggggc
      961 ccgtgcgcaa cgcgtcacta tgccaatgtt taatgccttc tggcagcaaa ataaactgat
     1021 cgaaatgcgc cgcagcgaga agaacgaaca gtacgttcgt tatggtgatt tccgcgccga
     1081 tccggtgaaa aatgcgctgg gtacgccaag cggcaaaatt gagatttact ccaaaacgct
     1141 ggaaaaattt ggctataagg attgcccggc acacccaacc tggcttgcgc ctgatgagtg
     1201 gaagggtacc gccgacgaga agcagttgca gcttctgacc gcacatccgg cacaccgttt
     1261 acacagtcag cttaactatg cggaactgcg taaaaaatat gcggttgccg atcgtgaacc
     1321 gatcactatt cacaccgaag atgctgctcg atttggtatt gcgaatggcg atctggtgcg
     1381 tgtctggaac aaacgtggtc agattttgac aggcgcggtg gtgactgacg ggatcaaaaa
     1441 aggcgtggta tgcgtgcatg aaggtgcatg gccagatctg gaaaacggct tgtgtaaaaa
     1501 cggcagtgcg aacgtgttaa cggcggatat ccccagctcg cagcgggcaa atgcctgtgc
     1561 cggtaactct gcgctggtgt atatcgaaaa atatacgggt aacgcaccga agttaacggc
     1621 atttgataag cctgctgttc aggcgtaaga agtgaatagc gcccggaaac gggcgctgag
     1681 gttatgcaaa cgtaaaactg cctgatgcgc tatgcttatc aggcctacat agtccctgca
     1741 atagattgaa ttagcaaggg tttgtaggcc ggataaggcg ttcacgccgc atccggcagt
     1801 aataccagac ctttacggct tgcgcgcaat cagcaccgcg cgtttaggcg caggataacc
     1861 ttcaactgtc ttactcggat catgtgggtc gaggaaatcg gacagagatt cggtaaccat
     1921 ccattcagtg cgtcgctgct cttcagtagt ggtaacgcac acatccacaa tgcggatatc
     1981 tacaaaacca cacttcttca gccagttttt cagcgccagc gcggaaggga tgaaatagac
     2041 attacgcatt tgcgcgtaac gatcgcctgg taccagcacc gtgttctcat cgccatcaat
     2101 aaccagcgtt tccagcacca gttcaccctc attcaccagt tgatctttca actgccagag
     2161 atgctccagc ggcgaacggc gatggtaaag cacgcccatc gaaaagacgg tatcaaaggc
     2221 tttcagtgcc ggaagctgtt caatacctaa cggtaacaaa tgtgcgcgct gatcgttacc
     2281 cagtagttta cgcaccgctt caaactggca cagaaacagt tgcgtagggt cgatccccac
     2341 cgcgaggtgc gcgcctgcgc caatcatgcg ccacatgtga taaccgctgc cgcagccgac
     2401 atccagaatg gtgcgtccgg ttaaatcaga aagatggggc agaacacgat cccatttcca
     2461 gtcggaacgc cattcggtat cgatgttgac gccatacagt gagaacggcc ctttgcgcca
     2521 tggcatcagg ttccgcataa gcgtttcaat gcgtttaatt tgcccggtgc tcagtggctc
     2581 ttcgctttcg gcggttacgc tatgcaataa atccagacga tacggtttaa tttcaggcag
     2641 aaattccacc gtgttggacc actgcttaaa cagcccgtgc tgctgctcgc gctgccagtt
     2701 agcaatctgc gcgggcagcg tttcgagcca gtgtgaaaga tgatttttgg caatcagaga
     2761 ataaaagtta ccaaagtcga tcatgcagcg tcctctgctt ttaatgccac cagtgaacca
     2821 aagttaaagc actggaacca cagctcgcta tgctcaaaac cggctttatg caggcgtgct
     2881 ttatgggttt ccacggaatc ggtcagcatc acgttttcca gcatgctgcg cttctggctg
     2941 atctccagtt cgctgtaacc gttggcacgt ttaaagtcgt ggtgcatgtt gaacaacagt
     3001 tcaccaactt tggcatcttc gaaactgaat ttttccgaaa gcaccagcgc gccgcccggg
     3061 ttcagtcctt gataaatttt atccagtaat gcctggcgct cggaaggttc caggaattgc
     3121 agggtaaaat tcagcaccac catcgatgcg ttttcaatgg cgatatcgcg aatatcacct
     3181 tcaatgacgt ctactggcgt aggggcttta taggcgtcaa tatgacgacg gcagcgttca
     3241 atcatcgccg gggagttgtc gatggcaata attttgcaat tatcatgatg aatgttgcga
     3301 cgcaccgaga gcgtcgccgc gcccagagaa caacccagat cgtaaacctg cgtaccaggt
     3361 tgaacgaagc gctcggctaa cataccaatc atggaaataa tattggaata gccgggaacg
     3421 gaacgctgga tcatatccgg gaagacttca gcgacccgtt catcaaaggt ccagtcgccc
     3481 agtctggcga taggggcaga aaatagcgtg tcgcggtgag acataacgta aaaatccggg
     3541 aaaaagaaag tggcgtattg tgcgctaacg cagggagaaa accaactccc agggcatata
     3601 ccaaagattc gccagcacca tcagcaacag cgcacaccag gtggcgctca tgccggaacg
     3661 tcgccagcga aacagacggt gatgaaaacc gtaataatgc atcagacgac cagcaagcaa
     3721 aacgatgccg caaatatgca ccatccaggt ttctgcgcca ttcatttcca taaacagcat
     3781 cagcacaatc gcgatgggaa tatattccac cgcgttacca tgaatgcgaa tagcgctttg
     3841 cagttcgcta aaaccaccgt caccataggc aacgcggtac tgcattcgca ggcgaacgac
     3901 atcaaaagag aacttcatta ataacaacgc acttaaaacg gcatatagcg cgcttaccat
     3961 acaaactccc tttaaaatgg ccgatggcac ctgtctatga taggtggcaa attcagaaaa
     4021 gagaagattg ctgtggaata gccggaactg ctccgatctc tggaagcgtt ttacgtaagt
     4081 cttcccacag g
//
LOCUS       DAJTUN010000210         4080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTUN010000210 DAJTUN010000000
VERSION     DAJTUN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.213"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(75..230)
                     /locus_tag="OO470_003826"
     CDS             complement(75..230)
                     /locus_tag="OO470_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6252906.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(433..840)
                     /locus_tag="OO470_003827"
     CDS             complement(433..840)
                     /locus_tag="OO470_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6252907.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            917..1144
                     /locus_tag="OO470_003828"
     CDS             917..1144
                     /locus_tag="OO470_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6252908.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1128..1550
                     /locus_tag="OO470_003829"
     CDS             1128..1550
                     /locus_tag="OO470_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252909.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1628..2411
                     /locus_tag="OO470_003830"
                     /pseudo
     CDS             1628..2411
                     /locus_tag="OO470_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2418..3164
                     /locus_tag="OO470_003831"
                     /pseudo
     CDS             2418..3164
                     /locus_tag="OO470_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3179..3601
                     /locus_tag="OO470_003832"
     CDS             3179..3601
                     /locus_tag="OO470_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6252910.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3659..4015
                     /locus_tag="OO470_003833"
     CDS             3659..4015
                     /locus_tag="OO470_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252911.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT         1088 a          864 c         1123 g         1005 t
ORIGIN      
        1 gctccagttg ctggaggcgt tttgcatctt ctaggagtag agcgataacg tgttttaatt
       61 ctgtctcgtt cattttactc acctgaatgt cttcccaacc aacgacgtgc gccagcttcg
      121 gttttaaacg ttttgctttt ggtatacgtt atggcggtga atgtgccgtc ctggttgggg
      181 aacacgccgt acaccggaga ttcgttgttg ccaagatcga tagtattcat gttgacccca
      241 tttcccctta acgccggggt agcggaactg tttgctgaga acaccgtgcg gtgtcttgat
      301 gaataaaatt tagaataacc taaggagggt gatcaagatt tttatgtaga aaaacctaag
      361 ttttttgatg taaaaaacac aagtgtttga aagtttgtgc tttttattac agggtgtgga
      421 gaaaaaaggg gattatttgt ttgcgcttct tttgcgagct ttgagtagtt cttcaaaaag
      481 tttgttgaaa ttttcaactc gagcacgcat ctctgacaac agggcctttt gctctgactc
      541 aggcagtgcg tcgaacagtt gaagtaactc tttttgatct tctgtcagaa tggctggctg
      601 attatccggg atcggttcgc ctggttgttt atcttcatcc ccaaaaagaa gccaagtcgg
      661 tgagcactga agcgcttggc tcagtgcgaa taatcttttc cccgccggct gtgtttcatc
      721 tctttcccat tgagaaattg ttacgtgagc cactttgacc agcttaccta atgcggcctg
      781 agacagtttt aattttttac gcctatgtaa gaggcgagca ccgaaggttt cgtttttcat
      841 attagggaat tctaattttt cttgacttag gtttctctac ggcctggttt ccttaggaaa
      901 atctaaggag ctcgatatgt tgaaaattga tgctatagcg ttttttggca gcaaaacaaa
      961 gcttgccaat gccgcaggag ttaggttggc aagcattgct gcatgggggg aactggttcc
     1021 tgaaggtcgc gcgatgcgcc tgcaagaggc atctggcggg gaacttcagt acgaccccaa
     1081 agtttatgac gaatatcgta aggcaaaacg acctgggaag gtgattcatg aaaatcaggc
     1141 atgagcacat cgaatcagtg ctgttagcct tggcagccga aaaagggcag gcgtgggtcg
     1201 ctaacgcaat tactgaagaa tatctgcggc aggggggcgg cgaattgtct ctggtaccag
     1261 gcaaggactg gaacaatcag cagaatatct atcaccgttg gttgaaaggt gaaacgaaag
     1321 cgcaaaggga aaaaattcag aaactgatcc ctgcggttct ggcaattctt ccgcgtgagc
     1381 tgcgtcaccg actctgtatc ttcgataccc tggaacgccg tgcattactg gcggcgcagg
     1441 aagcgttgag tacggcaatt gatgcgcatg atgatgctgt ccaggccgtt taccggaaag
     1501 cacatttcag cggtggtggg tcgcccggcg attctgtcgt agtgcattga ttgaaattaa
     1561 tcgtgccgga ttgttttgtt cggtatcagt taaatgtaac gctgcgagcg ttacaaggtg
     1621 aaaacaaatg gcttcaaact ggataaagct cgaggttatt acgccggata agccggaaat
     1681 attcaggctt gctgagattc tgaatattga tccagatgcc gcattaggga aggttattcg
     1741 cttctgggca tgggcggatc aacaaatgat agacggtaat gcagattgta acgctcgcgg
     1801 cgttacaaaa agtgcaatag atcgcatcac ttttatgtct ggttttgctg atgcgttaat
     1861 tcaggttgga tggctggtcg aaaatgacgg tgggctttct ctacctaact ttgaacgtca
     1921 taacggaaaa agctctaaaa aacgggcggt tacaaacgag cgagtaacaa aaatacgcga
     1981 actgaaacga aaaggtaacg ctgccagcgt tacacaaacg gatcaaaaag cgttaccagt
     2041 ggaagaggaa gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca
     2101 aaaacgagcg tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga
     2161 aacactctgg catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga
     2221 gcagggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga
     2281 ttcgacacag catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg
     2341 agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct
     2401 tcagggggta acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc
     2461 gactgacccc gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc
     2521 aacttgctga aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg
     2581 tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga
     2641 attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg
     2701 atgaactgat gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga
     2761 atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg
     2821 ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgatggg cagtcaggcg
     2881 aaaaattttt gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc
     2941 agcgcgagac gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt
     3001 cgatgcgcag cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg
     3061 gcgagcgggt gatggatcgc atggtcatga acggcgggcg ctgggttaat tttaactggg
     3121 agagctggcg tccgaatgtt agccattcga gggttgttaa gtagtttcag gaggatttat
     3181 ggcgaaacct tttactcccg aacagcggga agaactgaag acgcgaattg tggaactcgt
     3241 gcatcaggac ggtcgggtca cgattcggca gttgtccgat gaaacaggta tcagtcgtgc
     3301 gtctgtcggt cgcttatgca tagaactggt cgcaagtggt gatgtatata attctggcta
     3361 cggcttattc ccgtctgaac aggctcgcaa ggactggcaa agcgcccgca aaaaactctc
     3421 gagagtaaag gtgaggaaac cggttgttgt tgatccggac cttatctggt cattacctga
     3481 cggagaaata cgccgctacg acaggcgcct gaatataatc tgtcgcgagt gccggaagag
     3541 cgaagctatg cagcgtgtac tggctttcta tcagggtaat tttcaggagg cgatactgtg
     3601 aatgaaatta gctatcaggc ttcaattacc gctggcattc gcatcaaagg agaggagcat
     3661 ggaaataaaa ccagaagatg agttaagcaa tatcgtttta tttccgataa aagaggatga
     3721 ccctcgtaat caggttaatt ttctttataa gccatcggaa agaccatatt gccatcacgc
     3781 ctctgtacgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg gtgcggttgt
     3841 ggagccgttt gactggatgc tctctgtggc aaaaagagaa accagactgg cagatgatgt
     3901 aaggctattg cgccaggagg aacaggaaag gcggagaaat atagaaaagc tgatacagat
     3961 tgagcgtaac gcgaaagcgc ggatacgcag ggcgacaaaa tccagaactg aataattaaa
     4021 tttagcactg ttaaaaattt aatccttaac cggagggatt tctgcaccct caaatcatca
//
LOCUS       DAJTUN010000211         4063 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTUN010000211 DAJTUN010000000
VERSION     DAJTUN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.214"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(57..218)
                     /locus_tag="OO470_003834"
     CDS             complement(57..218)
                     /locus_tag="OO470_003834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252912.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(215..403)
                     /locus_tag="OO470_003835"
     CDS             complement(215..403)
                     /locus_tag="OO470_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6252913.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            663..998
                     /gene="iraM"
                     /locus_tag="OO470_003836"
     CDS             663..998
                     /gene="iraM"
                     /locus_tag="OO470_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6252914.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1279..1387)
                     /locus_tag="OO470_003837"
                     /pseudo
     CDS             complement(1279..1387)
                     /locus_tag="OO470_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1910..1986)
                     /locus_tag="OO470_003838"
     tRNA            complement(1910..1986)
                     /locus_tag="OO470_003838"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1950..1952),aa:Arg,seq:tct)
     gene            complement(2088..2163)
                     /locus_tag="OO470_003839"
     tRNA            complement(2088..2163)
                     /locus_tag="OO470_003839"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2128..2130),aa:Ile,seq:cat)
     gene            complement(2308..3129)
                     /locus_tag="OO470_003840"
     CDS             complement(2308..3129)
                     /locus_tag="OO470_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6252915.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3144..3500)
                     /locus_tag="OO470_003841"
     CDS             complement(3144..3500)
                     /locus_tag="OO470_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6252916.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3513..>4063)
                     /locus_tag="OO470_003842"
     CDS             complement(3513..>4063)
                     /locus_tag="OO470_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6252917.1"
                     /translation="WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTA
                     TSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKR
                     WVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEK
                     HGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1118 a         1096 c          802 g         1047 t
ORIGIN      
        1 acccactgac actaccggtg agtttaacga taccttccat ttgactggct cactttttat
       61 gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc atacgttgca
      121 tttttggtaa tatccgtcag cgtcagtgca tttagtccca ctgccagact gtcttttatg
      181 gcctggaatg ccgggccagt acgatgacgt agtatcactc cggctcagtt gcaccactga
      241 ccaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt atggccaccg
      301 gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc gcaagatctg
      361 acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt tccaacatct
      421 gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa accatacctt
      481 aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg agatataaac
      541 caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct ctatggttta
      601 attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa caaggaaact
      661 aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata tctttttcag
      721 tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc ttacctcctg
      781 aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg ctacctgtaa
      841 ccatttacaa cgtagtacca ttcaataaaa cattctggaa tttaatcaaa aacagccagg
      901 aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt tgcactctgc
      961 aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca catctgacaa
     1021 aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt gccattccat
     1081 ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat tatcccatat
     1141 acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt cttttaacaa
     1201 actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta aaaatttctc
     1261 ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg ggaaagcaac
     1321 aaccaccaca cccgccacca gcacaccgtc agccggcact gacattatcc ggctgctgca
     1381 atgccattaa caaaaacagt aagcaatcac tttttaccgt aacaggtgat aatccagata
     1441 tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct ggctattctg
     1501 ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc atcgcaaacc
     1561 ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg atcaggagta
     1621 cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac tatatcaata
     1681 cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc cacaccatat
     1741 gaatgataaa tacttaatgc tgaaaaaacc attagaccgt ataacaaata ctcaatcaat
     1801 acttaacaga acttttattt ttgacaaata tataatattt tcaacaatat cctgagccag
     1861 gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat ggcgcgctgt
     1921 actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa
     1981 caacgctgaa taccgataat ggaccgccat cggggacccg ccccccgcac caacaaccct
     2041 gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg tggcccttgc
     2101 tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg
     2161 gccgggagca gaataataac ggtccgtaat taatttcgca ataaaaaaac ccgctcagcg
     2221 gcgggttgta gaaactcttc taacgccagg cataaaacgc ccatcattat gacgaattta
     2281 ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact gccgctcagc
     2341 ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct taactgtttt
     2401 ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa atgcctccgt
     2461 tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga taacaggcat
     2521 accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc aatccttaca
     2581 ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta ccacctccct
     2641 gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact ttgcccatac
     2701 cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt caataaattt
     2761 ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat aatcctcaaa
     2821 agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa gttttctcaa
     2881 cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg ttgcccgcgc
     2941 cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca tctcagcccg
     3001 attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat cttttgacgt
     3061 tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg gtaaatcttc
     3121 cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac ccccagacgt
     3181 ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc atcgtccatt
     3241 aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc caggtcgcga
     3301 cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga caggcttaat
     3361 ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt tccctcctcc
     3421 gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg cgggtaaggt
     3481 aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt tgcaaaggcg
     3541 tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc gaacgccaga
     3601 ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtga
     3661 gatttggtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc gtcggctggc
     3721 ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc gttaacccag
     3781 cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac ggcaatgctg
     3841 accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag cggcgcaaga
     3901 ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac gatgtcgctt
     3961 tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg taataccgct
     4021 tccggtagcg cgtccgccac ctgattgcgg accgcccacc agg
//
LOCUS       DAJTUN010000212         3996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTUN010000212 DAJTUN010000000
VERSION     DAJTUN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.215"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            69..1028
                     /locus_tag="OO470_003843"
     CDS             69..1028
                     /locus_tag="OO470_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6252918.1"
                     /translation="MFVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAIS
                     LIQQRPLTGWGLGSFEATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGF
                     LLLSGVWLWPCICRLKMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLL
                     LLLFRIGMPENVMNQVRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADM
                     NEGFIPRLPEHSWKYLTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHN
                     DADVSAAMIFIAHRRGDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1028..1702
                     /locus_tag="OO470_003844"
     CDS             1028..1702
                     /locus_tag="OO470_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6252919.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2230..2991
                     /locus_tag="OO470_003845"
     CDS             2230..2991
                     /locus_tag="OO470_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6252920.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3035..>3996
                     /locus_tag="OO470_003846"
     CDS             3035..>3996
                     /locus_tag="OO470_003846"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6252921.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTV"
BASE COUNT         1120 a          757 c          978 g         1141 t
ORIGIN      
        1 tcgtgctggc cgctctcgcg gacagacccc gctggcgcgg acgtcccctt agcggtctgt
       61 tgcgcttgat cttcgtcgcc tccttgctgt gctggtgtgt cggtatctca aactatgcca
      121 tgccagggga gacaacggct gactttacgc attcatcatc gaatgaccag cgtatacgta
      181 ttctgaaagg tgccataagt cttattcaac agcgcccctt aaccggttgg gggttaggca
      241 gctttgaagc gaccttcccg caagccttag aggatgtggg cgtgacaaac atagagagtg
      301 atacggctac gcatccccat aacgaactgc tgttcgtaac ggctgaaggt ggcatcgtcg
      361 ccttgtttgg ttttttgctg ctatcaggcg tttggctgtg gccgtgtata tgccgcctga
      421 aaatgacaaa caataacgcg caccatcaat gtgaaaagtg gtttattgcc ttaatggggt
      481 tgcctttagt cattcatatg atgaccgaat atcctctcta tacgtctgtt ccccatttga
      541 tgctgttgct gctccttttc cgcatcggaa tgccggaaaa cgtgatgaat caggtacgta
      601 tacctaaaac agtccggttg ggtattctgc ctgtcattgt gctcattatg accgccgcgc
      661 tggtcatcct ggtagcggga tttgatgcac agcgggaact gaccagagct gaagctgata
      721 tgaacgaagg atttatccct cgccttccgg aacatagctg gaaatacctg acgcaggcag
      781 aacgtcttga gcgcgatcag catctgctta ttgcgaataa accggggttc gttcgcgata
      841 cggaaagcat gtctcttttc actgtgtggg gaaataaatg gttggcaatc cacaatgatg
      901 cagacgtaag cgctgcaatg atattcattg cgcacaggcg cggtgattat ctgacagctg
      961 aaaaaataag aaaacgcgca gctcgggtat ttgtaaatga cgaacgtttt actcgcgggg
     1021 gaaactaatg gttaacgatc tgacgtataa gaaattagaa agcacaaaca tcaactgtaa
     1081 tattctctcg ccagaaaata ccacatggac aaatgtcacg gtgattggcc tggatctttt
     1141 tgccatttgc ggcgtgtgca gtttgctcaa gcgtgttggg ggagtgaaac ttcaggtttt
     1201 cacttcctta cccactgata tgaatgttct gactgacagt agcatcgtga tatgggtaaa
     1261 aatgcgtcac gatggtttgc ctgaattagc cggacatatt gtgaaggttt gtcgtcgata
     1321 ccgtctgatg cggcaactcg ttattagcga tgctattcct gaaggaatga cgaatgatat
     1381 cgggcctctt tcccgagtat ggcttactca tgggagtgca aaagtggata ttttattatc
     1441 ttcattacgg aaggtgttaa atgctcattg tttgcctggt cctttgttta taaaacgcct
     1501 gggaagagtg caatggaggg ttcttttact ccgcgcgaag gggattagca cacgtaaaat
     1561 tgcagaaatc tgtggtattg gcctcaagac cgttagcgcc catgagtccg caattcgaga
     1621 acgattaggc accaggaata aaagtgagta cgtctggtta gtacgtaatg ctcaacttat
     1681 acaacaggcg atacccgcgt agggaagaga tgcgtcaagg tatgaaagat gtaacaatat
     1741 gcgaacaaag caaaaagtat aattgatgcg tcatagataa ctctcttttt cgatgacgaa
     1801 atcgctgagc atgcgtggtg aggttagtaa tagatgggtg ctatgttttg ccacgggttt
     1861 gtagctgtga aacctggaaa aaaataagaa ttaaaaatat ttaaagagag ttaataaggt
     1921 tctggttgta attgtcttgg tcaaaaaaaa gatattgtca atgttgtttt ttaaattttc
     1981 aggttttggt taaatatttg cttacaaata taggtgattt ttttattgta ggcttttgaa
     2041 ggctattata actgtgcgct aaattaatgc gttgaataaa agaaataaca aaattaagaa
     2101 aagccatatt tacgatatgg gcaacataaa tataatctaa aaaagagtaa ggcgttgtag
     2161 gttggttccg cgttcattac cttgttagtg aacgcggatt tatcagttgt cgaaatggtt
     2221 tttataataa tgaaaaattt taatgtacta accaaagtcg tattttgctt gtcgttttta
     2281 tcaagcgcat ttcccaccta tgctgcggat gatgtcaaag tgactcaaaa cacttcagtt
     2341 tttaaactga agcttagtgc aacgcgatta atctatgaac aagggtcgga tggcgcagcg
     2401 ataactgtga caaatccgca ggattggccg atgcttgtgc agagtaaagt ctttgaagaa
     2461 gataaagtaa ctgcagcgcc ctttattgtc actccgccac tgttcaggct taatccaggc
     2521 cagcaaaata aaatgtgggt tgtgcagact gcgccagctt tacaagacag ggaaaaatta
     2581 cactggctat gcgccaccgg cattacgcct aaaaagagtg atgtttgggc cgagaaagat
     2641 aaagaaaaaa ttgctgaaaa tgtgaacctg aacatccagg ttaacgtaca tacctgcata
     2701 aaacttatta ccaggccatc tggtattgaa ggcaagatgg ttaatgcgag tgagaagtta
     2761 tcgtggaaaa agcagggtga taaaattgtc gcggtcaatc agtcgccgtt ctacatcaac
     2821 ctttcttctt tacaggttgg cggtagcaag attgagaaac tagattatat tgcgccgttt
     2881 tcgcaacatg agtttgttat gccaaaaact agcggccagg atattaaatg gtctgtgatt
     2941 actgatgaag gtggtgagag tcgtttgttt atatctcatt tgcaatcata atgtattaca
     3001 tttttattga atatttttat gccatgcata ttccatgttt tatcgactgt cattaatttt
     3061 gataatggcc ttacttgcgg gtcagttaag tgcccaggag tggtcatttg attcttcaca
     3121 gttggaagga aacgttagtg cggacactgt ggcaatgttt aatcagggag agcaactccc
     3181 cggaaattat cgtgtggaaa tttatcttaa cggtgaaaaa gttgatgttg gggagtttcc
     3241 ctttcatcgt ccggaatccc ctgaagaaaa agagcttgtt ccatgtttaa ccgttgatga
     3301 ccttatccac tatggcatta aaattgataa aagcagcagt gatactgaca ataaaaagaa
     3361 tcagtgtttt aaatggaact ctattgaagg tttgaaagta aattatgatt ttgatagcca
     3421 gcgcgtacag ataaccgttc cacaactgta tctgcaagat aaaaaaagta gtttagcacc
     3481 agtgtcgtta tggaatgagg gtgtagctgc attccgcatg gtgtatcaga caaatattga
     3541 tatttccaaa caaaatgata atcaatctac cacgcgtaat tctcgttatg gcaggttcac
     3601 tccagggttt aatcttggtg cctggaggtt tcgtagttca gtgacatgga gcaaggaact
     3661 tggccagtca gaaagatggc aaaggggtta catgtggttt gagcgaggca taaatgcgat
     3721 taaaagccga ttaacgcttg gagaatctta tacgtcgtct gaggtgtttg acagcatacc
     3781 ttttcgcggc gggatgttag cgaccgacga tgcgatgact ccgccggagg acagctacta
     3841 cacacctgtg gtgcatggaa tagcacaaag tgaggcgcag gttattatta aacaaaatgg
     3901 gcaaattatt tttacccgtt ctgtgccgcc agggcctttt gcgctggata atttaccaac
     3961 gctggccgtt ggcggggagc ttgatgttac agtgag
//
LOCUS       DAJTUN010000213         3989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTUN010000213 DAJTUN010000000
VERSION     DAJTUN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.216"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..1248)
                     /locus_tag="OO470_003847"
     CDS             complement(<1..1248)
                     /locus_tag="OO470_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6252922.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSV"
     gene            complement(1333..1668)
                     /locus_tag="OO470_003848"
     CDS             complement(1333..1668)
                     /locus_tag="OO470_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6252923.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(1668..2024)
                     /locus_tag="OO470_003849"
     CDS             complement(1668..2024)
                     /locus_tag="OO470_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6252924.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2024..3520)
                     /locus_tag="OO470_003850"
     CDS             complement(2024..3520)
                     /locus_tag="OO470_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6252925.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(3517..3678)
                     /locus_tag="OO470_003851"
     CDS             complement(3517..3678)
                     /locus_tag="OO470_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6252926.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(3688..>3989)
                     /locus_tag="OO470_003852"
     CDS             complement(3688..>3989)
                     /locus_tag="OO470_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252927.1"
                     /translation="VHDVRRELWKALLGWMPDPQGGEIVYVGGTLLDLNRYELYYQFD
                     FTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEHHLEMRFPQN"
BASE COUNT          898 a         1210 c          915 g          966 t
ORIGIN      
        1 cacgctaccc atgatttttg ttatgcgtgt caatgtgcca atcgccacgc ccatcgtcag
       61 taatgccgca ccagttttcg cgatggtttt tacgacctca ggattttctc tgacaaacgt
      121 gcgcacttct tccagaaaag gcttcatctc ttttatgcct tcattgagtg aaggcaggaa
      181 ggtttccccc agcgtggaag aaatcgcatt gatctggttc tgcagcagca acaactgatt
      241 ttctgtcgtc gcggcacggg cggcatattc tttctgcatt gagccgccat actgctgcgc
      301 atccgccaca cgattaaagt tggttcgcaa caaatccaga ttcgtgagca gcggcgcaat
      361 ggcccccagg gattccttcc cgaacagcgc tttcagcaca gcagcctgtt tttctttcgg
      421 cacttttgcc agagaatcca gtacgtgcag catggccccg cgggcgtctt tctgcatatc
      481 cgccgccagt tttttcgggc taatgcgcaa cgagcggagc acttttttct gcgatttggt
      541 ggcagaatcc ctcgctgtca gcgacaacat aaaattcttt atccccgtcg cagcaatttc
      601 tgactccacc cccattccgg caatggttgc ccccattgcg gcaatctctc cggaggccac
      661 acccgcaaca ctgcctaaag ggccaatacg ggtcacaaca tcagaaatct ttttcgcact
      721 tgcaggaccg gtattgccaa gatagttaat cttatccgca agccctgcca cctctccctg
      781 tgtcagttta aaggcagtgc gccactgcgc catcatctga ccggattctt ctgccgtggt
      841 gtcaaaggcc acgcccatct tcacggcatc gtcagtaaac tgcatcagct cgtcacgggc
      901 gataccagcc tgaccacccg ccgccacaat ttcggcgata ccttccgccg acatgggcag
      961 ttctgttgac aggtcgcgca cctgctccgt cattgcctta aacgcttccg gcgtatccag
     1021 accatccacc actttccgga catccgccat ttttgattca agggcaatgg cagatttgac
     1081 cgggagcgcc agcgccccca gtactgcggt tccggcaccc gctgcgccca gtgacaggct
     1141 ggcaaactcc tttttaaaac ctttcagctg gcgctgcatt cccttcagcg gtgccgatac
     1201 cttatccacg gcagtgatga tcgccttcag ctgaaaactg tcagccatgc ttcatctcct
     1261 cgttaatgcg gacggcctcg gcttccagtt ctgtaaactg cgaaacagcc acccgtcgca
     1321 gctccagtgg attcagtttc cagaaccagg caacattgta gagtcgtttc cggaggtgct
     1381 tcccgtctcc gactgggtaa aaaaacgcag gatctgcatg ctggtcttaa aaatatccag
     1441 ttttgccatc tgcgccgccg atgaacacgg gatccccgcc agcaacggga tatatttcag
     1501 tgccacctgg ctgtccagtt taatactgcc ttcaccggaa ataatgaaag gaaaacccag
     1561 cgcctcgatt tcgtcatacg tgggctcgcg caactccagc acatgcagca tttcgttatg
     1621 cgctgtcacc ggttttttta aaactatttc tgtaacgttc attgccatta ctgataccct
     1681 ccctcttcgc cgtggaattc aagatctgcc gtgccttctt cggcattatg attagcctct
     1741 ccgtgcagcc atgccgccga aagcacatac accatgccgt ttgccagctc agcggtaatc
     1801 gtcatctggt ctgaggtggt aattttgtcg accggaaagt ttttgggcac tttaaacgtg
     1861 cccttaacgt aaggtgaccg ccaggtctcc ttgtaatcca catcccctgc catgccgacg
     1921 atgtcatccc ggacattggt gttcatcggc acttcaatgc cacctgtcag cgataactgc
     1981 tgaccatcca ctttaaaaaa acaggttccg gcgatctttg ccattatgct gactcctctg
     2041 aatactgaag acggaactgg ttaaccaccg caaagacacg taactgatta acataatccg
     2101 gcgggaacag cgtattcagt cggttcgggt tatctgcatc acgctcaact atcagatact
     2161 gtttaaacag atcgtaattt tccacaatac cggcacgctc catctgacga tatgtcgcca
     2221 gaagttcccc tttgataacg gcaggagtga caatcgcctg ccccggacca aaacgggtac
     2281 catcatttgc cagcttgtga cgtccgtact tgcttgtgat gaccgatttc agtttgcgaa
     2341 gaacatatgc actggtatgc agagtttcac tgtccagata gctgttgtct gccacgccat
     2401 acgcattctt tttgtaggtg gttacggaac gctggatctg caacgtgccg ccttccacat
     2461 aagccgtcgc cacgccgtga gataaaaggg tctgctgctc tgtcatgatg aatcgcttac
     2521 ctttcggtgc cggaagcatc cccaccagct cccccgtctg tgtcggacgg gcaggatcat
     2581 tccggataaa taccgcttca cgggcaaggc gactggcaac cagttcatcg acaggcgact
     2641 gggtttcttt ttcgtaaccg gcaagcgtga tatgttgctg attatgcata tctccggcac
     2701 caaccagctc tgacagcgtt cccagttttg cggtatagac atgcccgtat aactggcgcg
     2761 cataactcca gcgaccgctg ctgtcattca tttcggtcat catcatattg atggaggcgg
     2821 catcgttgaa cggcagaccg ataaagtcga atgcctcatc gcccatagca gcaacagcgg
     2881 cggtaagatc aggcgctccg ctaccggcag caccgacttc cgtcaccacc cgaagccctg
     2941 ctggcaaaat ctcaccacca ccagaaccat aataattcag acagaccggt aactcattac
     3001 catacagccc cttatgacgg gcagtcagcg tcaccacgcc tgcatcagat gacgccgtaa
     3061 acggcagggt gataaccccg tttaccgctt ccttaatcgc ggtggcaacc gcagtggcat
     3121 catcgccatt caccacaggc acctgtacac gggagcgccc gacatacaga ctcagggtgc
     3181 cgctttcctc tgcttctccg gcaaccgtca ccctgaccgt cgccgccgcc cctctggctt
     3241 ccggtactgc aataacatac agttcaccga aaggatctgt ctgacggtag acttccacca
     3301 tacgcgccag ctggctcccc gccccacaaa tctggcgggc ataatctgcc gatggcatca
     3361 gcaccaggct gttaacctca atggctgcat cgttgctggc atgcccgatc agtaatgcag
     3421 gcgcgctggt caccgctgta tttgccgcag aattgttcat ctcggcgtag aacaacggca
     3481 ccagcgtatt cgacggaaca gtattaaaac ttatcgtcat gattttttag ccttatcctg
     3541 tttaacacgt accacatcac ccgccgctat acggcggaac cagtagctgc tttcttccac
     3601 atttcgccct tcggaaggca aaaggtctcc acgggcaggg tcaggaaccg accgcccttt
     3661 caggggtttc acaaacatga gggactctta attctgaggg aaacgcattt ccagatggtg
     3721 ctcaatgtca ccatccgggc cagtaccagg atcgatgtaa tccacatcaa tactcagcag
     3781 ggataaatcc ggcagggcat tcacgtcctc tgcctgtcgc gtgtcttctt ccgtgatttc
     3841 atacttcgcc gtaaaatcaa actggtaata cagttcgtaa cggttcagat ccagcagggt
     3901 gccaccgaca taaacaatct cgcccccttg cgggtccggc atccatccaa gcagggcttt
     3961 ccagagttct ctccggacat catgaacgg
//
LOCUS       DAJTUN010000214         3956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTUN010000214 DAJTUN010000000
VERSION     DAJTUN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.217"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            13..696
                     /locus_tag="OO470_003853"
     CDS             13..696
                     /locus_tag="OO470_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6252928.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            693..1154
                     /locus_tag="OO470_003854"
     CDS             693..1154
                     /locus_tag="OO470_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6252929.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1167..2339
                     /gene="iadA"
                     /locus_tag="OO470_003855"
     CDS             1167..2339
                     /gene="iadA"
                     /locus_tag="OO470_003855"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6252930.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            2404..3315
                     /gene="hypT"
                     /locus_tag="OO470_003856"
                     /gene_synonym="qseD"
     CDS             2404..3315
                     /gene="hypT"
                     /locus_tag="OO470_003856"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6252931.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(3308..3700)
                     /gene="iraD"
                     /locus_tag="OO470_003857"
     CDS             complement(3308..3700)
                     /gene="iraD"
                     /locus_tag="OO470_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6252932.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(3697..3780)
                     /gene="idlP"
                     /locus_tag="OO470_003858"
     CDS             complement(3697..3780)
                     /gene="idlP"
                     /locus_tag="OO470_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6252933.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT          922 a         1030 c         1036 g          968 t
ORIGIN      
        1 atggggattg ttatgacaca acaaggggat gctgtagccg gggagctggc gacagaaaaa
       61 gtgggcatta aagggtatct ggcttttttt ctcaccataa tcttttttcc cggtgttttt
      121 tccggaacag acagctggtg gcgcgttttt gatttttccg ttctgaacgg atcgtttggg
      181 caactgcctg gtgcgaatgg agccaccacc tcatttcgcg gggtgggtgg cgcgggggct
      241 aaagatggct ttctctttgc gctggagctg gcaccgtcag tcattctctc tctggggatt
      301 atttcaatta ccgatggtct tggtggatta cgcgccgcgc agcagttaat gacacctgtg
      361 ctaaaaccgt tgttgggcat tccagggatc tgctcgctgg cactgatcgc taacctgcaa
      421 aacacggatg ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga aattaccgaa
      481 cgcgataaag tgattttcgc cgcatatcaa accagcggta gcgccatcat caccaactac
      541 ttctcttccg gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc
      601 gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct taacttcgaa
      661 gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca
      721 tggatatgtt tatcgatggc gcgcgccgtg gctttactat cgccacgacc aacctgctgc
      781 caaacgtggt gatggcgttt gtcatcattc aggcgctgaa aattaccggc ctgctcgact
      841 gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg attatccggt gaagcggcaa
      901 ccgtcctttt agccgcatta atgagtatgg gcggcgcagt cggtgttgcc gccagtctgg
      961 caaccgctga cgcattaacc ggccacgatg tcaccgtttt actgcccgca atgtatttaa
     1021 tgggcaaccc ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg aatgccaaat
     1081 attacccgca tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc
     1141 agcttattgt ttaacaagga gttaccatga ttgattatac cgcagccggt tttaccctgc
     1201 tgcagggagc gcatttgtat gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta
     1261 acggcaaaat tatcgccgtt gccagcaata tcccttctga cattgtaccg aactgcacgg
     1321 ttgtcgatct cagcgggcag atcctctgcc caggttttat tgatcaacac gtccatttga
     1381 ttggcggtgg cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc
     1441 tgacggaagc gggcgtcacg tcagtggttg gtctgctggg caccgactct atctctcgcc
     1501 acccggaatc cctgctcgcc aagacccgtg cgctcaatga agaaggcatc agcgcctgga
     1561 tgctgaccgg cgcttatcat gtcccttccc gcactattac gggttccgtg gaaaaagacg
     1621 tggcgattat cgatcgtgtg attggcgtga aatgcgccat ctctgatcac cgttctgccg
     1681 caccggacgt ttatcacctg gctaatatgg cggcagaatc ccgcgttggc ggtttgctcg
     1741 gcggtaaacc tggcgtcacc gtgttccaca tgggcgacag taaaaaggcg ttaaagccta
     1801 tttatgacct gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta
     1861 accgcaacgt accgttgttt gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg
     1921 atatcaccag cagcattgac gaaccggtcg cccctgccga aggtattgcc cgcgccgttc
     1981 aggcgggtat tccgctggca cgcgtcaccc tcagctccga cggcaacggt agccagccgt
     2041 tcttcgatga cgaagggaat ttaacccata tcggtgttgc aggttttgaa acgttgctgg
     2101 aaaccgtgca ggtgctggtc aaagactatg atttcagtat cagcgatgcc ctgcgcccgc
     2161 tcaccagtag cgtagccggt ttccttaaca tgagcgggaa aggcgaaatt ctgccaggca
     2221 atgacgcaga cttactggtc atgacgccag aattgcgcat tgagcaggta tacgctcgcg
     2281 gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa
     2341 cggttgtcag ccgccctaat ggacgtagaa tgcccccaag ggcggctgac agagtaaaac
     2401 gtaatggatg actgtggtgc ggttttgcat aatattgaaa ccaaatggct ttatgatttt
     2461 ctgactctgg aaaaatgccg caatttttcc caggcggcag tcagtcgcaa cgtctcgcaa
     2521 ccggcattca gccgccgcat ccgtgcactg gaacaggcga ttggcgttga attgtttaac
     2581 cgccaggtga cgccactgca actctcagaa caagggaaaa tcttccattc gcagatccgc
     2641 catctgttgc aacagttaga aagcaacctt gcagagctgc gtggcggcgg cgattacgcg
     2701 caacgtaaaa tcaagatagc cgctgcacac tctctttccc tcggtctgtt accgtccatt
     2761 atcagccaga tgccgccgct ctttacctgg gcaattgaag ccattgatgt cgatgaagcg
     2821 gtcgataaac tgcgtgaagg gcaaagtgac tgtatttttt cctttcacga cgaagatttg
     2881 ctggaagcgc cgtttgacca cattcgctta tttgaatctc aattgttccc tgtctgcgcc
     2941 agtgacgaac acggagaagc actttttaac ctcgcgcagc cacactttcc gttactgaat
     3001 tacagccgca actcctacat ggtgcgattg attaatcgca ccctgacgcg ccacagtgag
     3061 ttaagtttca gcaccttttt tgtctcttcg atgagcgagc ttttaaagca ggttgccctc
     3121 gacggctgtg ggattgcctg gctgccggag tatgccatcc aacaagaaat tcgcagcggg
     3181 cagctcgttg tgctgaatcg ggacgaacag gtgatcccga ttcaggccta cgcataccgg
     3241 atgaataccc gcatgaatcc agttgccgaa cgcttctggc gtgaactgcg cgagctggag
     3301 attgtgctta gctgacattc tcaagcgtcg cactgcgcca accgccatta tcccagacca
     3361 gatcaaacgt tagcggttca tcgtcgtggt acagcattat ctcaaggctg atcgccagag
     3421 tgtggcagta cgcttctttc aaattaacct gcaacgccgc aatccgtggc tcatgaaata
     3481 acagtgcaga acgcagctga cggcaatacc agctctggag atgtttgtcg ctcgggttat
     3541 gcacttgcca ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat
     3601 taaaaagagt gaggagatcg ctgcataccg atttacgcag caatgacgtt ttattgcctg
     3661 aaatttcagg taaaacagcc tgaagtgatt ggcgcatcat tttgcgcact cctgacgttt
     3721 agcaacatat gcggcttgcc ctgaacaccg ggcaccactg tattgatgct cattttccat
     3781 agccattccc ttttgttcat tacatccatg tcagtctgag acaggcctga atcctgtcag
     3841 caaagtataa aaccttactt gcctatgaat atctactgac ttttcaggaa aaaagtcatg
     3901 atttgataaa aatcatcaaa ctcaaaaaaa aaacgctagt atattctgaa tattcc
//
LOCUS       DAJTUN010000215         3931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTUN010000215 DAJTUN010000000
VERSION     DAJTUN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.218"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..522
                     /locus_tag="OO470_003859"
     CDS             <1..522
                     /locus_tag="OO470_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HCR6252934.1"
                     /translation="LPSFSISYAGNMVGVALTTEGECGLDMELQRATRGFHSPHAPDN
                     HTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLNDDPRDLQLLPIAGRLKCA
                     HVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWKSLPDIHSRANNPTSRMMR
                     FAQLSTVKSFSPN"
     gene            544..1110
                     /gene="yieF"
                     /locus_tag="OO470_003860"
                     /gene_synonym="chrR"
     CDS             544..1110
                     /gene="yieF"
                     /locus_tag="OO470_003860"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6252935.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1164..2501)
                     /gene="adeP"
                     /locus_tag="OO470_003861"
     CDS             complement(1164..2501)
                     /gene="adeP"
                     /locus_tag="OO470_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6252936.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            2666..3322
                     /gene="yieH"
                     /locus_tag="OO470_003862"
     CDS             2666..3322
                     /gene="yieH"
                     /locus_tag="OO470_003862"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6252937.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            3389..3862
                     /locus_tag="OO470_003863"
     CDS             3389..3862
                     /locus_tag="OO470_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6252938.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1046 a          982 c          985 g          918 t
ORIGIN      
        1 ttgccttcgt tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa
       61 ggtgaatgtg gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac
      121 gcgcccgata accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat
      181 cctaacgaag cgcgggcgca gctcattacg ctgcgccgaa gcgtgctaaa actaaccggt
      241 gatgttttga atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa
      301 tgtgctcatg taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc
      361 gtggcggtca cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc
      421 tggaaaagcc tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt
      481 tttgcccagc tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc
      541 attatgtctg aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt
      601 aatggcatgg ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg
      661 ttaccttcca ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt
      721 ccagcaacgg ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc
      781 acgccggaat ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc
      841 cgcctgccgg atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatgggc
      901 gtgattggcg gcgcgcgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca
      961 atggtgatga acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa
     1021 accggagaag tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt
     1081 ggtgagttta ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc
     1141 gggtttttaa tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac
     1201 agcagcgcaa cgatgattac gcacgggcta aggtcacgca gacgcccggt accaatcttc
     1261 atcacgcagt aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc
     1321 atcatcacgg cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga
     1381 gcaagacttg aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagcct
     1441 ggcaccattc ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc
     1501 actgccgtca gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc
     1561 gtaacggaag aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc
     1621 gcctgcttca tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg
     1681 ccaatcagcg taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct
     1741 gccagcccga ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc
     1801 ggcggcgcag aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc
     1861 gtcaccacga tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg
     1921 atgaaaaagc cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcatc
     1981 agcgtttccg agttagcgac aatcacacct gcgtttttca gccccatcat gccgataaac
     2041 aggccgatac cgctggtaat acccacacgc agacttaccg gaatgttagc aatcatccag
     2101 taacgaacgc ggaaaatggt caaaagaagc agtccgacag cgccccagaa gattgcgccc
     2161 ataccgatct gccacggcag ccccattgcc tggacaacca caaaagcgaa aaaggcgtta
     2221 agccccatcg caggagccag cgccaccgga aggtttgcaa acaatcccat cataatgcta
     2281 ccgaaagcag caatcagaca ggttgttacg aagacggcac tggtgtccat gccagcaacg
     2341 cccagaattt gtgggttaac aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg
     2401 gcaatcactt cagttcgtgc cgtagtacca tgttcgcgca gtttaaaaac acgttccagc
     2461 atcctctgac cagaagtctg ggtggtatgt tgatgactca ttatctattt ccgaacaagg
     2521 agggaaaatc cgtcgctatc gtataccaaa atgcgacaat aggcgcgttt gtgagagact
     2581 tttttattgg atttacttat acggcaacga ttgcgttgcg caaatcggct ctacgaaaac
     2641 gttaaactga ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt tcgactgcga
     2701 cggtacgctg gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga
     2761 atttggtatt acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta
     2821 cgaaattatc gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga
     2881 acacgtttac cgtgcagaag tcgctcggct gttcgattca gccatcgaag gggctggagc
     2941 gctcctgtca gcgatcactg cgccaatgtg tgtggtatct aacggcccaa ataacaaaat
     3001 gcagcattct atgggcaagc tgaatatgtt gcactacttc ccggataaac tgttcagcgg
     3061 ctacgatatt cagcgctgga agccagaccc ggcgttaatg ttccatgcgg caaaagcgat
     3121 gaatgtaaat gtagaaaact gcattctggt tgatgactca gttgccggtg cgcaatctgg
     3181 tatcgacgca ggtatggaag tgttctactt ctgcgccgac ccgcacaata agccgatcgt
     3241 tcacccgaaa gtcaccacct ttacccatct ttcgcagtta ccggaactgt ggaaagcgcg
     3301 tggttgggat attacggcat agttcttcac actcccttca cttaccccgc ttaaattggc
     3361 gctcaaaggt aagtaaaggg agtttgatat gtctgtttca cgtcgggtaa tacatcacgg
     3421 actttatttt gcggttttag gaccgttaat tggtgttctg tttcttgtcc tctacatatt
     3481 cttcgcaaaa gaaccgctgg ttcttttggt aataatacag gtacttccat tgtttctctt
     3541 attgtcgatt actacgggag ctattcctgc attgttaacc ggcgtgatgg tcgcctgtct
     3601 gccggaaaag atcgggtcac agaaaaatta tcgttgtctg gctggtggca taggtggcgt
     3661 cgttatcacc gagatctatt gtgcagttat tgtacatatt aagggcatgg cttcctcgga
     3721 gttgtttgaa aacattcttt ctggtgacag tctcgttgtc cgcatcattc ctgcattgct
     3781 ggcaggtgtg gtgatgagca gaatcattac ccgtctgccc ggattggata tttcatgtcc
     3841 tgaaacagac tctttaagtt aagcgggata ctttatcttt gggctactca aaagcagaca
     3901 ggatgttact atgactcaaa atatcaggcc g
//
LOCUS       DAJTUN010000216         3881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTUN010000216 DAJTUN010000000
VERSION     DAJTUN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.219"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..378)
                     /locus_tag="OO470_003864"
     CDS             complement(<1..378)
                     /locus_tag="OO470_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6252939.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAP"
     gene            574..1341
                     /locus_tag="OO470_003865"
                     /pseudo
     CDS             574..1341
                     /locus_tag="OO470_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(1392..2044)
                     /gene="pphB"
                     /locus_tag="OO470_003866"
                     /pseudo
     CDS             complement(1392..2044)
                     /gene="pphB"
                     /locus_tag="OO470_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2150..>3881)
                     /gene="mutS"
                     /locus_tag="OO470_003867"
     CDS             complement(2150..>3881)
                     /gene="mutS"
                     /locus_tag="OO470_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6252940.1"
                     /translation="EITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVL
                     LERQQTIGALQDFTAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELR
                     AQLETVDSAPVQALREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWR
                     ALADGATDYLERLEVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTL
                     KNAERYIIPELKEYEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELD
                     VLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLI
                     ITGPNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRST
                     FMVEMTETANILHNATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFAT
                     HYFELTQLPEKMEGVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEV
                     IKRARQKLRELESISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQAL
                     EWIYRLKSLV"
BASE COUNT          933 a          987 c          993 g          968 t
ORIGIN      
        1 tggcgcatcc agcatttcca gatcgaatcc agccagagcg gtagcaattt cttgcgcatc
       61 agcactagcg atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac
      121 gcctgtttca ccaaacagca cctgtttaac ctgggccgca ttgaccacca gcaccagcag
      181 agcgtccagt ttttcggcaa acgtcgcggc gttatcagaa accccgcaag cacctgcctc
      241 tttcaacgta gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa aaactgcgcg
      301 gacacatgac agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa
      361 ctcagatccc gttttcatct gctctccttg ttaatttaag tgatattttg tttgatattg
      421 tgaacataag cgctgggaga taacgatatg gtgagatgat tcacataaat taacattttg
      481 tgttatttta tgtgaactaa gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt
      541 agaatgtcag caggacaaag gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc
      601 atccttgaga tggtagctga aaaaggcatt gtcagtattg ctgaactaac ggacagaatt
      661 aatgtgtcac atatgaccat tcgtcgggat ttacaaaaac tggagcagca gggagccgtt
      721 gtgctggtgt ccggaggcgt ctagtctccg ggacgcgtgg cgcatgaacc ttctcatcag
      781 gtaaaaactg cgctggcaat gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt
      841 gttctgccgg gaagttgtat ctatctggat gcgggaacga ccacgttagc gatagcacag
      901 catctgattc acatggagtc actgactgtg gtcacaaacg atttcgttat tgcgaactac
      961 ttgctcgaca acagtaattg cacaattatt cacactggcg gtgcagtgtg tcgggaaaac
     1021 cgttcctgtg tcggggaagc cgctgcgacc atgctgtgca gcctgatgat tgatcaggct
     1081 tttatttctg catcgtcatg gagtgtgcgg gggatttcta cgccagcgga agataaagtc
     1141 acggtgaaac gggcgattgc cagtgccagc cgccagcgag ttttggtctg tgatgcgacg
     1201 aaatatggtc aggtggcgac atggctggcg ttaccattaa gcgagtttga tcagattatt
     1261 acagacgacg gtttgccgga gagtgccagt cgcgcgctgg cgaagcagga tctctctttg
     1321 ctggtagcga aaaatgaata atggcctgca ataacatctg gttactcatg cttcacagaa
     1381 gaagcatgag actaccttat tttataaaat gacagccgcc cacttttcgg cgatccggta
     1441 tcaatataaa tctggttagc gaacgtctga atgttatcaa acatcatatg tccaaatata
     1501 aaataatcag cgccgtttat ttgttgtaac tcgccattaa gcgatttctg cacacgataa
     1561 acaggccaga gtaattcgct ctccgctatt tctttgccaa agagatattc atcacccgga
     1621 taatctgcat gtgcgatgac atattttatt atgtcgttag tgatttcaat aatatgtgga
     1681 aggtgatgga atttcagcaa cagatctatt gcctcttgtt gcaatcattt aaatcgaaaa
     1741 accagtcacc accgctggca agccacatat tgccatcgcc ggtcgcgaac gcatccagcg
     1801 ccattgcttc gtggttgcct ttaacggaga taaaccaggg ctggtttagc aggcgcagga
     1861 cgtcaagact ctccggccca cgatcaatat tatcgccgac agaaataagt aagtcggttt
     1921 cggggaaaaa agagagttga tgtaagcggg attgtaataa ctgatattca ccatgaatat
     1981 caccaacgac ccatatatgg cgatagtgat gggcattgat tttttggtag cgtgtagatg
     2041 gcatggtttt accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg
     2101 tcagttgtcg ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac
     2161 tcttcaagcg ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat
     2221 tttccagtgc ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg
     2281 taccatccac ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt
     2341 gccgtgcgcg cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt
     2401 agcttttgct cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct
     2461 ccagcgcatc gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct
     2521 cgaaatagtg ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc
     2581 acgcccacgc cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta
     2641 acaccagact gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca
     2701 taaaggttga gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat
     2761 cgataggtcc aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg
     2821 caatcagtgc ggtctggcgc atataggtac ttttaccgcc catgttcggg ccggtaataa
     2881 tcaacatacg gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta
     2941 cttgttcaac taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg
     3001 tcgggcaggt gtagttcagg gtataggccc gttccgccag gttaaccagc acgtcgagtt
     3061 ccgccagcgc gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca
     3121 gctcttcata aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat
     3181 cttcgtactc ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac
     3241 gcatgtagtt gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt
     3301 gcaccgcatt aaagccaact tttagcgtgt ccaggccggt acgttcacgc tcgcggactt
     3361 ccagacgctc cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt
     3421 cgttatagcc cgatgcgata acaccaccgt cgcgtaccag caccggcggt gtgtcgatga
     3481 ttgctcgctc cagcagatcg cgcagctcgg caaactcgcc catcttctca cgtagcgcct
     3541 gtaccggtgc actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag
     3601 cgtggcgcat acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa
     3661 tacgttccag gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca
     3721 atgcgccaat agtttgctgg cgctcaagca acacgcgggt atcgcgcact ggcatatgca
     3781 gccagcgttt cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca
     3841 gcgtattttc cgcaccgccc gccaggttct gagtaatttc c
//
LOCUS       DAJTUN010000217         3843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTUN010000217 DAJTUN010000000
VERSION     DAJTUN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.220"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            24..1439
                     /gene="aldA"
                     /locus_tag="OO470_003868"
     CDS             24..1439
                     /gene="aldA"
                     /locus_tag="OO470_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6252941.1"
                     /translation="MYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDA
                     AERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTAD
                     YIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALL
                     TGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVS
                     MTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQV
                     CNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVA
                     RAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDA
                     ISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIG
                     GADGKHGLHEYLQTQVVYLQS"
     gene            complement(1481..2482)
                     /gene="gap"
                     /locus_tag="OO470_003869"
     CDS             complement(1481..2482)
                     /gene="gap"
                     /locus_tag="OO470_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6252942.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2671..3201
                     /gene="cybB"
                     /locus_tag="OO470_003870"
     CDS             2671..3201
                     /gene="cybB"
                     /locus_tag="OO470_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6252943.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3446..3619
                     /locus_tag="OO470_003871"
     CDS             3446..3619
                     /locus_tag="OO470_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252944.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3691..3840)
                     /gene="hokB"
                     /locus_tag="OO470_003872"
     CDS             complement(3691..3840)
                     /gene="hokB"
                     /locus_tag="OO470_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6252945.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT          983 a          871 c          988 g         1001 t
ORIGIN      
        1 tgtcagtacc cgttcaacat cctatgtata tcgatggaca gtttgttacc tggcgtggag
       61 acgcatggat tgatgtggta aaccctgcta cagaggctgt catttcccgc atacccgatg
      121 gtcaggccga ggatgcccgt aaggcaatcg atgcagcaga acgtgcacaa ccagaatggg
      181 aagcgttgcc tgctattgaa cgcgccagtt ggttgtgcaa aatctccgcc gggatccgcg
      241 aacgcgccag tgaaatcagt gcgctgattg ttgaagaagg gggcaagatc cagcagctag
      301 ctgaagtcga agtggctttt actgccgact atatcgatta catggcggag tgggcacggc
      361 gttacgaggg cgagattatt caaagcgatc gtccgggaga aaatattctt ctgtttaaac
      421 gtgcgcttgg tgtgactacc ggcattctgc cgtggaactt tccgttcttc ctcattgccc
      481 gcaaaatggc tcccgctctt ttgaccggta ataccatcgt cattaaaccc agtgaattta
      541 cgccaaacaa tgcgattgca ttcgccaaaa tcgtcgatga aataggcctt ccgcgcggcg
      601 tgtttaacct tgtactgggg cgtggtgaaa ccgttgggca agaactggcg ggtaacccaa
      661 aggtcgcaat ggtcagtatg acaggcagcg tctctgcagg tgagaagatc atggcgactg
      721 cggcgaaaaa catcaccaaa gtgtgtctgg aattgggtgg taaagcaccg gctatcgtaa
      781 tggacgatgc cgatcttgaa ctggcagtca aagccatcgt tgattcacgc gtcattaata
      841 gtgggcaagt gtgtaactgt gcagaacgtg tttatgtaca gaaaggcatt tatgatcagt
      901 tcgtcaatct gctgggtgaa gcgttgcagg cggttcaatt tggtaacccc gctgaacgca
      961 acgacattgc gatggggccg ttgattaacg ccgcggcgct ggaaagggtt gagcaaaaag
     1021 tggcgcgcgc agtagaagaa ggggcgagag tggcgttggg tggcaaagcg gtagagggga
     1081 aaggatatta ttatccgccg acattgctgc tggatgttcg ccaggaaatg tcgattatgc
     1141 atgaggaaac ctttggcccg gtgctgccag ttgtcgcatt tgacacgctg gaagatgcta
     1201 tctcaatggc taatgacagt gattacggcc tgacctcatc aatctatacc caaaatctga
     1261 acgtcgcgat gaaagccatt aaagggctga agtttggtga aacttacatc aaccgtgaaa
     1321 acttcgaagc tatgcaaggt ttccacgccg gatggcgtaa atccggtatt ggcggcgcag
     1381 atggtaaaca tggcctgcat gaatatctgc agacccaggt ggtttattta cagtcttaat
     1441 gagtgaaaga ggcggaggtt ttttcctccg cctgtgcgcg tcagagttta gcgaattttt
     1501 cgagggtgcg aataagctgc gtgacgaagc catattcgtt atcgtaccag gcgaccgttt
     1561 tcaccagttg taaatcgccc acggcggtaa tttccgtttg cgtggcatca aacactgaac
     1621 cgaaatggct gccaatgata tcggaagaga ctatttcttc atcggtataa ccaaatgact
     1681 cgttattggt ggttgcttgt ttaagtgcgt tattcacctc ttcggcagtc actttttttc
     1741 cgagaatgga caccagctca gtgaccgaac ctgttttcac cggcacgcgt tgcgcatgac
     1801 ctttcagttt gccgctcagt tccgggatca ccagaccaat ggcttttgcc gcccccgtag
     1861 tgtggggaat gatattttct gccgctgcgc gtgaagcacg taaatcttta ccacgcgggc
     1921 catccaccag tgactgggtg ccagtatagg catgaatggt cgtcatcgtg ccgacttcta
     1981 tcccgaaact gtcatgcaag gctttggcca tcggcgcaag gcagttagtg gtgcatgacg
     2041 ccacggaaac aatggtgtcg ttgccatcca gagtgtcgtc attaacgtta taaacgatgg
     2101 ttttcatttc accggcagga gcggaaatca acaccttctt cgcaccagca tcaagatgcg
     2161 cctgcgattt atcggcggag gtataaaagc cagtacattc gacaatgatt tctgcccctt
     2221 tcgctttcca cggaatattt ttagcctctt tttcggcgta aaccgcgata cttttcccat
     2281 caacgataag tgaatcttcc gtaaaatcaa cgctccaggg gaacggacca tagtttgaat
     2341 catgtttcag cagataggcg agaatttttg gggaagtaag atcattaata gcgacaacgt
     2401 ctatgttgct tttgacttca agtaaacgac gcaacaccag tcgaccgata cgaccaaaac
     2461 cgttaatacc aactttactc atggttttct cctgtcagga acgttcggat gaaaattgat
     2521 cctttccagg cttagaccag gattgtggga tgggcaatct ccattcttac agtgaaacgt
     2581 aacgtactga aaacgggtga acaatattta atgaaatttt gagaaaagcc cgttatgtta
     2641 acggaaaatt atgttaaagc aggaaatgtt atggaaaata aatactcaag gttacaaatc
     2701 agcattcact ggctggtctt tttgctggtt atcgcagcgt attgcgcaat ggagtttcgt
     2761 ggtttcttcc cacgtagcga tcggccgctc atcaacatga ttcatgtttc ctgtggcatc
     2821 tcaattctcg tgctgatggt ggttcgtctt ttgttaagac tgaaataccc aaccccgccg
     2881 attataccta agccgaaacc gatgatgacg ggactggcgc atttgggaca tttggtgatt
     2941 tatctcctgt ttattgcgct gccagtgatt ggtttggtga tgatgtataa ccggggcaac
     3001 ccgtggtttg cgtttggttt gacgatgcct tacgcttcag aggccaattt cgaacgggtt
     3061 gatagcttaa agtcgtggca tgagacgctg gcgaatctgg gatattttgt catcgggttg
     3121 cacgctgcgg ctgcactggc acaccactat ttctggaagg acaacacact tctacgcatg
     3181 atgccgcgta aacgttcctg aaggatattt aaagaaaacg cctgtactaa aaccgacccg
     3241 tggtacaggc gaagaatacg ggtctacatc ggaagcgcct attatattta tttgtatgac
     3301 aaataaaacc ataatccttg cccatacgtc catctggctt atttttaatc aattcacccg
     3361 atctttgatc tcatcaacgg tatcaaaata aaccagcgta taacgttcaa atcgcacata
     3421 agattcgata aaccatggag gttatatgaa aaaactggca cttattttgt ttatgggaac
     3481 gcttgtttcc ttttatgccg atgccgggcg caaaccctgt tctggttcga aaggggggat
     3541 ctcacactgt acggcaggcg gcaaatttgt ctgtaatgat ggttctatta gtgcatcgaa
     3601 aaaaacatgc actaactgaa gtgtaaaagg ggtgccatga gaaaatgaat ctgctagtca
     3661 gatgcgcggg gaaaatcccc gcgcttgccc ttacctggac gtgcaggcca tgagcgcagc
     3721 aacctcctta tcaccgtccc ggaaccgcag ttcgtagagc gtttgtcggg tcaggagtgt
     3781 gaatgccaga atcgtaatgc agataatgag cagacacacc accagagggt tgcgcttcat
     3841 agc
//
LOCUS       DAJTUN010000218         3747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTUN010000218 DAJTUN010000000
VERSION     DAJTUN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.221"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO470_003873"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO470_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6252946.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO470_003874"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO470_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6252947.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO470_003875"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO470_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6252948.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO470_003876"
     CDS             complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO470_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6252949.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT          998 a          951 c          911 g          887 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatat
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaacgca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtaggtaccg
     2881 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
     2941 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     3001 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
     3061 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     3121 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
     3181 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3241 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3301 gagcattatc tggcgacatc catgcatggg gattaggttt gccctcatag ggtccttcgg
     3361 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
     3421 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
     3481 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
     3541 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tccgcgatga
     3601 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
     3661 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtgc ataatttagt
     3721 acctatagtg agcaaaatta gaacaaa
//
LOCUS       DAJTUN010000219         3653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTUN010000219 DAJTUN010000000
VERSION     DAJTUN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.222"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            460..822
                     /locus_tag="OO470_003877"
     CDS             460..822
                     /locus_tag="OO470_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6252950.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            819..1739
                     /locus_tag="OO470_003878"
     CDS             819..1739
                     /locus_tag="OO470_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6252951.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1742..2693
                     /locus_tag="OO470_003879"
                     /pseudo
     CDS             1742..2693
                     /locus_tag="OO470_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2720..2960
                     /locus_tag="OO470_003880"
                     /pseudo
     CDS             2720..2960
                     /locus_tag="OO470_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            2957..3262
                     /locus_tag="OO470_003881"
     CDS             2957..3262
                     /locus_tag="OO470_003881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252952.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3496..>3653)
                     /locus_tag="OO470_003882"
     CDS             complement(3496..>3653)
                     /locus_tag="OO470_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6252953.1"
                     /translation="NVIAPLQDAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWP
                     KKPESNL"
BASE COUNT         1060 a          707 c          692 g         1194 t
ORIGIN      
        1 tggctggttc agtccggtgt ctctcttctt gcgcttaaag agatgggagg atgggaaact
       61 ctcgaaatgg ttcaaagata cgcccacctt tcagccgggc atctcaccga gcacgcgagc
      121 aaaatcgatg cgattataag tcgcaatggc acaaatacgg cacaagagga gaacgtagtt
      181 tacttaaatg cgaggtaact tattgattta aatggtgccg ataataggag tcgaacctac
      241 gaccttcgca ttacgaatta taagaatccg cttgtaattc aaaggattat cccatcaaca
      301 ctgcgctcac acgtcccacc acatcaaaac atgtaaagcc ttgcaagcca ttgtgaggcc
      361 ttatgtgtct cagttttgtc tcatcagaca tagcaagtat cgatcaattg agacttggat
      421 gatagacttc atgcctttca gagctcattg attaaataaa tgttaaagtt atttgtaaag
      481 tacacatcta taggtgtgct gaacacactt atacactggg tggtttttgg tgtatgtatc
      541 tataccgcac ataccagtca ggctctggca aacttcacag gtttcgtagt ggctgtgagc
      601 tttagcttct tcgcgaatgc aagattcaca ttcaaagcat cgactacagc gatgcgctac
      661 atgttatacg tgggatttat gggaatattg agtgtgattg ttggttgggc agctgataaa
      721 tgctcacttc ctccaatagt cactcttatc accttctccg ccatcagtct ggtgtgcggt
      781 ttcgtctatt caaagttcat tgtctttagg gatgcgaaat gaaaatatct cttgtcgttc
      841 ctgtcttcaa tgaagaagaa gcgatacctg ttttctataa aacggtacgt gaattccaag
      901 agttgaagcc atatgaagta gaaattgtat tcataaatga cggaagtaaa gatgccacag
      961 agtcaattat taacgcgctg gctgtttcag acccgctagt tgttccgctg tcatttacac
     1021 gcaactttgg taaagaacca gccttatttg cagggttaga ccatgcaagc ggcgatgctg
     1081 taattcctat tgatgtcgac ctgcaagacc caattgaggt tatccctcat cttattgaaa
     1141 agtggcaggc aggtgctgag atggtgcttg ctaaacgttc agaccgctca actgatggac
     1201 gactgaaacg taagacagct gagtggttct ataaattaca caacaaaata agcaccccaa
     1261 agatcgagga aaatgtcgga gattttcgac tcatgtctcg tgaggttgtg gagaacatta
     1321 aactgttgcc tgagcgcaat cttttcatga aaggcatact gagctgggtg ggtggtcaga
     1381 cggatgtcgt tgaatatgta cgcgcagagc gtgttgctgg catctcaaaa tttaatggct
     1441 ggaaattatg gaatctggca ctggaaggta tcacaagttt ttcaaccttt cctcttcgcg
     1501 tatggactta tataggcttg tttgttgcaa gcatttcatt tttatatggt gcatggatga
     1561 ttatagacac ccttgtcttt ggtaacccag tacgcgggta tccctccctg cttgtatcaa
     1621 tacttttctt gggtggagtg caactgatcg ggattggtgt tctcggtgaa tatataggta
     1681 gaatttactt agaaacaaaa tcaagaccta agtatatttt gaagaaaggt agaaaataac
     1741 catgagcata tgtattaaac aatcagcatt aaaaatatta ttggctttat cagcactcct
     1801 aataacttgg ttgactacca gatactttcc agttgaaccg gatgttgcta acagcccaat
     1861 tgtatggagg catattttag agaatggtat atcatcaatc cacgactgga aaccaaccgt
     1921 ggataattgg tatttcaccg tttacccaat taacttttta ttttatatgc tgttaggtga
     1981 tgatggctta gtagcattaa gattatcaac tgcagttttc tctattgcta tagtaatagc
     2041 agcgatgtta acgctacgta aggcattcgg gtttacccct gctttatttt ctattatact
     2101 tctatctcta ataccatatt tttcatacac ttatggtttt gtatcacatc ctttttctca
     2161 taattcaaca aacgctttcg gctttttatg cttgcttata tctgttttca acatacagta
     2221 taaaaatata tttatcactc tcctcttaag tttgacagca ttattttcca gcgtatccga
     2281 tccgtggttt acagctgcat tttttattcc actattaata tcttactttt tattctctgt
     2341 atgggataaa aaattattta agcatactgc attaatacta tttgcatgtt taatctctct
     2401 ttccaatgta ttgcaaaatc tacttaacat acctccccac caatttgaaa ttgtatcact
     2461 caatgatatg attttaaatg caaaatggtg cattctgtta ataggaaaaa gtcttaattt
     2521 attagttgtc gacaataacg caacatctta cgcctcattt gtcatttggt tcattgccat
     2581 tattacatct gcgtggtttg ttttatcgga caataaaaaa aacacatacc gtatttatat
     2641 tgtgttattt tcattattat ctatcgcagg tattgtctca tcattcatac tgagctataa
     2701 atcccctgat tatatcagca tgcgtttctt tatgaatgtt acctgtttcg cactgatatt
     2761 gtgctgtatc ggtacatcaa caaaagcaaa gatattattt tatctaatcg catttttatt
     2821 ttcgatcagt tcaattaaat cttacacaaa taatgcttca ccattacacg atcaggagaa
     2881 gattgttaaa tcatacattg attttttaaa aaaaaataat ctccattatg ggtacggctc
     2941 tttttgggat ttgtcaatga ctgtaaattg gttatctggt ggggacatac agataactcc
     3001 tgttttcttt aatgctgatt cggggaaaat taactttact ggcgtgagac aacagacact
     3061 ggcttcttgg catagcaagg aggcgttcaa ttctgctcct gagcgacagt ttatagcagt
     3121 ttccatagct aacgaacctg aacgatgtaa agaaatgaca tcctgccttg caggtattca
     3181 agaacaatta ggtaagcctg acgaagtttt gaactttgaa ggaagagtaa tattagtatt
     3241 taacaaaaaa cttaacctat agtaaattgc cccctaccat ataaaccaca ctaacgaatg
     3301 aataacccgt ctttttcgac gggttatatc cctatcagga ataaaaatgg agtggttgcc
     3361 gtatacaaca aaccctcatt tagagctata ttctagagta tatgagatac tataaagttg
     3421 aaggagctat cattagaaaa accatatgct gttataatta gatgtctcgc ttttcatcca
     3481 ataacgaatt ttctcttata gattgctctc tggttttttc ggccactctg tattttttat
     3541 atcaacacgc atcaatagtg cgcgatattt tttccatgcc agtaacagtt tcgtttcatc
     3601 atctgttgcc atatctaaat caaccgcatc ctgaagcggc gctattacat tcg
//
LOCUS       DAJTUN010000220         3584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTUN010000220 DAJTUN010000000
VERSION     DAJTUN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.224"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..440
                     /locus_tag="OO470_003883"
     CDS             <1..440
                     /locus_tag="OO470_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6252954.1"
                     /translation="QNAMRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIAT
                     RKQNLETEKIQAQIAVTQAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEA
                     IRLRGEALRDNPGLVALTTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(486..2477)
                     /gene="tkt"
                     /locus_tag="OO470_003884"
     CDS             complement(486..2477)
                     /gene="tkt"
                     /locus_tag="OO470_003884"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6252955.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            2755..3513
                     /gene="loiP"
                     /locus_tag="OO470_003885"
                     /gene_synonym="yggG"
     CDS             2755..3513
                     /gene="loiP"
                     /locus_tag="OO470_003885"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6252956.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
BASE COUNT          854 a          944 c          989 g          797 t
ORIGIN      
        1 tgcaaaatgc catgcgcaaa gccgttgttg ggccagtggt aattgacggg gtccagattg
       61 aaaatattga tttttctgac gcttatgaaa agtcgattga aaaccgaatg aaagcagaag
      121 tcgccattgc cacgcgtaag caaaatctgg aaacagagaa aatccaggcg caaatagcgg
      181 taactcaggc ccaggcagaa gcagacagta aactggcggc agccaaagta gaagcggaaa
      241 ctattcgcgt aagaggcgcg gcagaggctg aaactattcg tttaaaaagt gcggctgaag
      301 cagaagcaat ccgcctgcgc ggtgaagctc tgcgggataa tcccggactt gtagccctca
      361 ccactgccga acgttgggac ggtaaactgc ccgataccat gatccccggc agcacagttc
      421 cgtttatctc tactaagtaa aaaaagggcc gctttcgcgg tccttctcac aaaacgtctt
      481 actgattaca gcagcgcttt tgctttcgca acaacgttat caacagtgaa gccgaactct
      541 tcaaacagca gctctgccgg agcagattca ccgaaggtgg tcatgccgac gatagcgccg
      601 ttcaggccaa catacttgta ccagtagtca gcaatacccg cttctaccgc aacgcgtgca
      661 gtaaccgctt tcggcagtac ggattcacgg taagccgcat cctgcttgtc aaatgcgtcg
      721 gtagacggca tggacaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca
      781 gcaaccgcca gttcaacttc agaaccggtg gcgatgaaga tcagttccgg ctgaccagca
      841 cagtctttca gcacataacc accgcgcgcg atgtttgcca gctgctcttc agtacgttcc
      901 tgctgcgcca ggttctgacg ggagaggata agcgcagtcg ggccgtcctg acgctcaacg
      961 ccgtatttcc acgcgaccgc ggattcaacc tggtcacacg gacgccatgt agacatgttc
     1021 ggggtcacgc gcagagaagc gacctgctct accggctggt gagtcgggcc atcttcgccc
     1081 agaccgatgg agtcgtgggt gtaaaccatc acctggcgct gtttcatcag cgcagccata
     1141 cgtacggcgt tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaag
     1201 ccaccgtgca gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag
     1261 tggatgtagt tacctgcagc atcttcgttg attgctttag aaccagacca cagggtcagg
     1321 ttagacggtg ccaggtcagc agagccgccg aggaattcag gcaacagcgg gccgaacgct
     1381 tcgatagcat tctgcgacgc tttacggctg gcgattttcg ccggattagc ctgcagttta
     1441 gcgataaact ctttcgcttt ggcgtcgaag tcagacggca tttcgccttt catacggcgg
     1501 gtaaattcag ccgcttcctg cggataagct ttcgcgtaag ccgcaaactt ctcattccat
     1561 gcagattctt tcgcctggcc tgcttctttc gcatcccact gagcatagat ttcagacggg
     1621 atttcgaacg gtgcatattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg
     1681 cccagcggcg caccgtggga gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg
     1741 atggttttgc acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttcaactgcg
     1801 cgtttgatgg atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct
     1861 tcgaaacgca ttgctgtgtc gtcggtgaac cagccttcaa cgtgaccatc gatagaaata
     1921 ccgttgtcat cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa
     1981 acttcgtggg agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg
     2041 tcgacaatgt cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc
     2101 ataccgactg cgttggcaat accctgaccc agcggaccgg tggtggtttc cacaccagcg
     2161 gtataaccta cttccgggtg gcccggagtt ttagagtgca gctgacggaa gtttttcagt
     2221 tcttccatcg gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg
     2281 tggccgttgg acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt
     2341 ttcaggaaat cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg
     2401 tgaccggatt tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc
     2461 tctttacgtg aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact
     2521 tgacgacagc gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg
     2581 cgtcatgtac atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt
     2641 tgcgcaatct gctcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc
     2701 acctgcaaac ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa
     2761 attcgcgcct tattggtagc aatgagcgtg gcaacggtac tgaccggttg ccagaatatg
     2821 gactccaacg gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat
     2881 gcgcaggtga aaaccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt
     2941 gcgccagcca atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac
     3001 aatatcaacg gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt
     3061 gcaatggcta acggctgtat ccgcgtctat agcgggctga tggatatgat gacagataac
     3121 gaagtcgaag cggtgatcgg tcacgagatg gggcacgtgg cgttaggcca tgtgaagaaa
     3181 gggatgcagg tggcgcttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg
     3241 attgtcggca gtttatctca atcacaactt ggtgatctgg gcgagaaatt agtcaattcg
     3301 caattctccc agcgtcagga agcagaagcc gatgattatt cttacgatct tctgcaccaa
     3361 cgcggcatca gcccggcagg tcttgccacc agctttgaaa aactggcgaa gctggaagaa
     3421 ggccgtcaaa gctcaatgtt tgacgaccat cctgcatccg ccgaacgtgc ccagcatatt
     3481 cgcgatcgca tcagcgcgga tgggattaag taaagcctag tggtgtcgaa cgtaggtcag
     3541 ataaggcgtt cacgccgcat ctgacattaa tggcacattt gtta
//
LOCUS       DAJTUN010000221         3478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTUN010000221 DAJTUN010000000
VERSION     DAJTUN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.225"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            17..93
                     /locus_tag="OO470_003886"
     tRNA            17..93
                     /locus_tag="OO470_003886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51..53,aa:Ile,seq:gat)
     gene            136..211
                     /locus_tag="OO470_003887"
     tRNA            136..211
                     /locus_tag="OO470_003887"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:169..171,aa:Ala,seq:tgc)
     gene            395..3298
                     /locus_tag="OO470_003888"
     rRNA            395..3298
                     /locus_tag="OO470_003888"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3394..>3478
                     /gene="rrf"
                     /locus_tag="OO470_003889"
     rRNA            3394..>3478
                     /gene="rrf"
                     /locus_tag="OO470_003889"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          924 a          760 c         1063 g          731 t
ORIGIN      
        1 gcagtaaaac ctctacaggc ttgtagctca ggtggttaga gcgcacccct gataagggtg
       61 aggtcggtgg ttcaagtcca ctcaggccta ccaaatttgc acggcaaatt tgaagaggtt
      121 ttaactacat gttatggggc tatagctcag ctgggagagc gcctgctttg cacgcaggag
      181 gtctgcggtt cgatcccgca tagctccacc atctctgtag tgattaaata aaaaatactt
      241 cagagtgtac ctgcaaaggt tcactgcgaa gttttgctct ttaaaaatct ggatcaagct
      301 gaaaattgaa acactgaaca acgaaagttg ttcgtgagtc tctcaaattt tcgcaacacg
      361 atgatgagtc gaaagaaaca tcttcgggtt gtgaggttaa gcgactaagc gtacacggtg
      421 gatgccctgg cagtcagagg cgatgaagga cgtgctaatc tgcgataagc gtcggtaagg
      481 tgatatgaac cgttataacc ggcgatttcc gaatggggaa acccagtgtg attcgtcaca
      541 ctatcattaa ctgaatccat aggttaatga ggcgaaccgg gggaactgaa acatctaagt
      601 accccgagga aaagaaatca accgagattc ccccagtagc ggcgagcgaa cggggagcag
      661 cccagagcct gaatcagtgt gtgtgttagt ggaagcgtct ggaaaggcgt gcgatacagg
      721 gtgacagccc cgtacacaaa aatgcacata ctgtgagctc gatgagtagg gcgggacacg
      781 tggtatcctg tctgaatatg gggggaccat cctccaaggc taaatactcc tgactgaccg
      841 atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc cggcgagggg agtgaaaaag
      901 aacctgaaac cgtgtacgta caagcagtgg gagcacgctt aggcgtgtga ctgcgtacct
      961 tttgtataat gggtcagcga cttatattct gtagcaaggt taaccgaata ggggagccga
     1021 agggaaaccg agtcttaact gggcgttaag ttgcagggta tagacccgaa acccggtgat
     1081 ctagccatgg gcaggttgaa ggttgggtaa cactaactgg aggaccgaac cgactaatgt
     1141 tgaaaaatta gcggatgact tgtggctggg ggtgaaaggc caatcaaacc gggagatagc
     1201 tggttctccc cgaaagctat ttaggtagcg cctcgtgaat tcatctccgg gggtagagca
     1261 ctgtttcggc aagggggtca tcccgactta ccaacccgat gcaaactgcg aataccggag
     1321 aatgttatta cgggagacac acggcgggtg ctaacgtccg tcgtgaagag ggaaacaacc
     1381 cagaccgcca gctaaggtcc caaagtcatg gttaagtggg aaacgatgtg ggaaggccca
     1441 gacagccagg atgttggctt agaagcagcc atcatttaaa gaaagcgtaa tagctcactg
     1501 gtcgagtcgg cctgcgcgga agatgtaacg gggctaaacc atgcaccgaa gctgcggcag
     1561 cgacgcttat gcgttgttgg gtaggggagc gttctgtaag cctgtgaagg tgtgctgtga
     1621 ggtatgctgg aggtatcaga agtgcgaatg ctgacataag taacgataaa gcgggtgaaa
     1681 agcccgctcg ccggaagacc aagggttcct gtccaacgtt aatcggggca gggtgagtcg
     1741 acccctaagg cgaggccgaa aggcgtagtc gatgggaaac aggttaatat tcctgtactt
     1801 ggtgttactg cgaagggggg acggagaagg ctatgttggc cgggcgacgg ttgtcccggt
     1861 ttaagcgtgt aggctggttt tccaggcaaa tccggaaaat caaggccgag gcgtgatgac
     1921 gaggcactac ggtgctgaag caacaaatgc cctgcttcca ggaaaagcct ctaagcatca
     1981 ggtaacatca aatcgtaccc caaaccgaca caggtggtca ggtagagaat accaaggcgc
     2041 ttgagagaac tcgggtgaag gaactaggca aaatggtgcc gtaacttcgg gagaaggcac
     2101 gctgatacgt aggtgaagcg acttgctcgt ggagctgaaa tcagtcgaag ataccagctg
     2161 gctgcaactg tttattaaaa acacagcact gtgcaaacac gaaagtggac gtatacggtg
     2221 tgacgcctgc ccggtgccgg aaggttaatt gatggggtta gcgcaagcga agctcttgat
     2281 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
     2341 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
     2401 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
     2461 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
     2521 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctttaatg
     2581 tttgatgttc taacgtggac ccgtgatccg ggttgcggac agtgtctggt gggtagtttg
     2641 actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc
     2701 ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga
     2761 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa
     2821 cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc
     2881 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt
     2941 atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc
     3001 ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac
     3061 cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa
     3121 atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga gatgagttct
     3181 ccctgaccct ttaagggtcc tgaaggaacg ttgaagacga cgacgttgat aggccgggtg
     3241 tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga accgtgaggc ttaaccttac
     3301 aacgccgaag atgttttggc ggattgagag aagattttca gcctgataca gattaaatca
     3361 gaacgcagaa gcggtctgat aaaacagaat tagcctggcg gccgtagcgc ggtggtccca
     3421 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggt
//
LOCUS       DAJTUN010000222         3393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTUN010000222 DAJTUN010000000
VERSION     DAJTUN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.226"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..2014)
                     /locus_tag="OO470_003890"
     CDS             complement(<1..2014)
                     /locus_tag="OO470_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6252957.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNIT"
     gene            2222..3211
                     /gene="ldhA"
                     /locus_tag="OO470_003891"
     CDS             2222..3211
                     /gene="ldhA"
                     /locus_tag="OO470_003891"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6252958.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT          853 a          886 c          835 g          819 t
ORIGIN      
        1 ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac gtaacggggc
       61 cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca aaatcgacac
      121 cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg tgccctcccg
      181 cgcggaatcc ttgttcaggc gcagcagaaa atgcaacctg agcatacagt tcaccatcgc
      241 gtaagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt gattgttttg
      301 gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc cgaaccgggc
      361 caatctcacc agcatgtaaa tcgcctttaa agaggaaata ggtcggattg cgcccatcga
      421 cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg
      481 cgtccagtga cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg
      541 gcttgtcgag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt aactggtcga
      601 aaccggtgga gagatcggtc agcgtaatcg tgctgtcatg ccactcacct tttcctgcga
      661 catcccaggt ggcattcatc ggtgtaaaac tcccttttcc ccagtagcgc cactgccagc
      721 ggccagcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg aaatcgccca
      781 gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca cgttgggtga
      841 cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatcg atgactcttc
      901 cctttgaacg aagtaacgcg cctggctcaa aggtcagcgt tgggtcagac agacttccac
      961 ttagctgcgc aggtaaacgg gcatataaaa tgaggtccgc ctgtttcgct tcaccggtca
     1021 gctgcagagg cagttgactg ttatccatgc ttaattttcc tgggccaaaa ttaagcaccg
     1081 cgttgccctt acccgcttgc ccctgggtca gcacattcag tcgtccgctg accagagcgt
     1141 tctcaagccc tgcctgccag ttgtcgactt tgacacccag tcggccactc aaaggaaaac
     1201 ctgcatacag ccagctccag cgaccatcgc ttacggtcaa ttgttgacga gtaatttgcc
     1261 acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacgatcaat tgcccgctat
     1321 tttcctgcca gtccagctcg gcatccacca gtgacggttc ctgcggcaag tttaacgtcg
     1381 cagtagcatg cccacttaca ggaagtccat ccggcacgag cggcatagta aattccccca
     1441 ccagtttaac cggcggctga ttttcaaacg cgacgacatc cagttcgctg actgtaagtt
     1501 gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc agttgctgga
     1561 tatcagaggt taatgcgaga gagagttttc cctgccattc ctgccaggga gaaaaaatca
     1621 gtttatcgat attgatccag gtgttaggca gcatggactg ccactgcgcg agggtttttg
     1681 gagcagctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa tcaagttcta
     1741 ccgtgccgac gttcaataac cagcggctgg gatgtgaaag gctggcgttg gtgatatgcg
     1801 caagctgaca atctcccacc agataacgga gatcgggaat gattaaacct ttacgcgtaa
     1861 tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg tccgccagcg
     1921 tgggaaccca cagcccgagc gtcatcagca gcgtcaacgg cacaagaata atcagtaata
     1981 acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct gatttagcga
     2041 aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg aaggttgcgc
     2101 ctacactaag catagttgtt gatgaatttt tcaatatcgc catggctttc aattatattt
     2161 gaaattttgt aaaatatttt tagtagctta aatgtgattc aacatcactg gagaaagtct
     2221 tatgaaactc gccgtttata gcacaaaaca gtacgacaag aagtacctgc aacaggtgaa
     2281 cgagtccttt ggctttgagc tggaattttt tgactttctg ctgacggaaa aaaccgctaa
     2341 aactgccaat ggctgcgaag cggtatgtat tttcgtaaac gatgacggca gccacccggt
     2401 gctggaagag ctgaaaaagc acggcgttaa atatatcgcc ctgcgctgtg ccggtttcaa
     2461 taacgtcgac cttgatgcgg caaaagaact ggggctgaaa gtagtccgtg ttccagccta
     2521 tgatccagag gccgttgctg aacacgccat cggtatgatg atgacgctga accgccgtat
     2581 tcaccgcgcg tatcagcgta cccgtgacgc taacttctct ctggaaggtc tgaccggctt
     2641 tactatgtat ggcaaaacgg caggcgttat cggtaccggt aaaatcggtg tggcgatgct
     2701 gcgcattctg aaaggttttg gtatgcgtct gctggcgttc gatccgtatc caagtgcagc
     2761 ggcgctggaa ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg aatcagacgt
     2821 tatctctctg cactgcccgc tgacaccgga aaactaccat ctgttgaacg aagccgcctt
     2881 cgatcagatg aaaaatggcg tgatgatcgt caataccagt cgcggtgcat tgattgattc
     2941 tcaggcggca attgaagcgc tgaaaaatca gaaaattggt tcgttgggta tggacgtcta
     3001 tgagaacgaa cgcgatctgt tctttgaaga taaatccaac gacgtgatcc aggatgacgt
     3061 attccgtcgc ctgtctgcct gccacaacgt gctgtttacc gggcaccagg cattcctgac
     3121 agcagaagct ctgaccagta tttctcagac tacgctgcaa aacttaagca atctggaaaa
     3181 aggtgaaacc tgcccgaacg aactggttta atcttgcagc tcccctgcaa tccaggggag
     3241 ctgattcaga taatccccaa tgacctttca ttctctattc ttaaaatagt cctgagtcag
     3301 aaactgtaat tgagaaccac aatgaagaaa gtagccgcgt ttgttgcgct aagcctgctg
     3361 atggcgggat gtgtaagtaa tgacaaaatt gct
//
LOCUS       DAJTUN010000223         3383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTUN010000223 DAJTUN010000000
VERSION     DAJTUN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.227"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            41..1330
                     /gene="uraA"
                     /locus_tag="OO470_003892"
     CDS             41..1330
                     /gene="uraA"
                     /locus_tag="OO470_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6252959.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1380..2126
                     /gene="hda"
                     /locus_tag="OO470_003893"
     CDS             1380..2126
                     /gene="hda"
                     /locus_tag="OO470_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6252960.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2259..2618)
                     /gene="arsC"
                     /locus_tag="OO470_003894"
     CDS             complement(2259..2618)
                     /gene="arsC"
                     /locus_tag="OO470_003894"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6252961.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2639..>3383)
                     /gene="bepA"
                     /locus_tag="OO470_003895"
     CDS             complement(2639..>3383)
                     /gene="bepA"
                     /locus_tag="OO470_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6252962.1"
                     /translation="EILLTHPLPESRLADARNRANQMRPMVVQSSEDFYLAKARTLGM
                     YNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQPLLAAEPGN
                     AWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQPQEAANILN
                     RYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLLSSASSQVK
                     LGSLQQARYDARIDQLRQLQERFKPYTKM"
BASE COUNT          685 a          858 c          908 g          932 t
ORIGIN      
        1 gagtaaagcg cctataacac ataatacaga ggataatact atgacgcgcc gtgctatcgg
       61 ggtgagtgaa agaccgccac ttttacagac aatcccgctt agtttgcaac atttgttcgc
      121 catgtttggt gcaaccgtcc tggtgcccgt cttatttcat attaacccgg cgactgtact
      181 gttatttaac ggtattggaa cgctgctgta tctcttcatc tgtaaaggga aaattccggc
      241 ttatcttggt tccagctttg cctttatttc accggtattg ttactgttgc cgttagggta
      301 tgaagtcgcg ctgggcggct ttattatgtg cggcgtgctg ttctgcctgg tttcttttat
      361 cgtgaagaaa gcggggaccg gctggttgga cgtgctgttt ccacctgcgg caatgggcgc
      421 aatcgttgcc gtcatcggtc tggagctggc gggcgtagct gccggtatgg cgggtttact
      481 cccggctgaa gggcaaacgc caaactccaa aaccatcatc atctctatta ccaccctggc
      541 ggtcacggtt ttaggttccg tgctgtttcg tggtttcctg gcaattatcc cgattttaat
      601 tggcgtgctg gtggggtacg cgctctcttt cgcaatggga attgtcgata ccacgccgat
      661 tattaatgct cactggtttg cactgccaac cctctatacg ccgcgcttcg agtggtttgc
      721 cattctgact attctgccag cggcgttagt ggttattgcc gaacacgtag ggcacctggt
      781 agtaacggct aatatcgtca aaaaagatct gctgcgcgat ccaggtctgc accgttcgat
      841 gtttgctaac ggcctgtcga ccgtgatctc cggcttcttt ggctctacgc caaataccac
      901 ttacggagaa aacattggcg tgatggcgat cacccgtgtt tacagtacct gggttatcgg
      961 cggggcggcg attttcgcta tcctgctttc ctgcgtcggt aaactggctg ccgctatcca
     1021 gatgatccca ttgccggtga tgggcggcgt ttcgctgctg ctttatggtg tcattggtgc
     1081 ttccggtatt cgtgttttga tcgaatcgaa agtggattac aacaaagcac agaacctgat
     1141 cctgacttcc gtgattttga tcatcggcgt cagtggcgcg aaggtaaaca tcggcgcggc
     1201 ggagttaaaa ggtatggcgc tggcgaccat cgtcggtatc ggcttaagtt tgattttcaa
     1261 actgatcagt gtgctgcgcc ctgaagaagt ggtgctggac gcagaagacg cggatataac
     1321 agacaaataa tgtaccgacc gggcagtgtt gctgcccggt tcaaacatca tgggattctg
     1381 tggtaaactt ctcgcgattt tgtgaaatcc tggttgaggt atctctgaac acaccggcac
     1441 agctctcttt gccactttat cttcctgacg acgaaacctt tgcaagtttc tggccggggg
     1501 ataactcctc tttactggcc gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca
     1561 tctatctctg ggcacgcgaa ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg
     1621 aattgtcgca gcgtggcgat gcggtgggct atgtcccgct ggataaacgc acctggtttg
     1681 ttccggaagt gctcgacggt atggagcatt tgtcgctggt ctgtatcgac aacattgagt
     1741 gtattgcagg cgatgagttg tgggagatgg cgattttcga tctctacaat cgaattctgg
     1801 aatcgggcaa aacacggttg ttgatcaccg gcgatcgtcc accgcggcag ttgaatctgg
     1861 gattaccgga tctcgcgtcg cgtctcgact gggggcagat ctacaaattg cagccacttt
     1921 ctgatgaaga taagttgcag gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc
     1981 cggaagatgt ggggcgtttc ttgctgaagc ggctcgacag agaaatgcgc acgctattta
     2041 tgacgttgga tcagttggat cgtgcgtcga ttaccgcgca acgtaagctg accattccgt
     2101 ttgtgaaaga aattctgaag ttgtagataa ggtgtttatt gtcggatgcg gcgcgaacgc
     2161 ctgacctacg cgaagttaat ccatagtatt cgtaggccgg ataagacgtg gcaagcgtcg
     2221 catccggcaa tgtgcttcaa ctagcgatgg agcacatctt aacccacgat ttccagtacc
     2281 tgctccggtg gacgaccaat ccgcgctttg ccattcgcga ccataatcgg gcgctccatc
     2341 agctttggat tatccaccat tgcctgaatc agcgcctctt cgctaagcga actgtcagcc
     2401 aggtttagtt ctttataaag atcctctttc tggcgcatca attcacgggc gctattcatc
     2461 cccagcattt tcagcaaatc gcgcagcgtt gccgcatcgg cgggtgtctc aaggtagagc
     2521 accacttctg gctccacgcc gttttctttc agcaggttca gcgtttcccg gctctttgaa
     2581 cagcgtgggt tatggtagat ttttacctgt ttggtcattg ttcttccttt aatgcgaatt
     2641 acatcttggt ataaggctta aagcgttcct gcagctggcg caactggtcg atgcgcgcat
     2701 cgtaacgcgc ttgttgcagg ctgcctaatt ttacctgcga actggcgcta ctcaacagcg
     2761 aaatggcctg atcgagtcgt ccggcgagcg cataaccttc tgctcgcgca gccagctcct
     2821 gatcgcggtt atttagcgcg gcttccgcct gtgccagcaa atcccagccg ttgctgtcat
     2881 ctttattatt aaaggtgtag cgattcagaa tattggccgc ttcttgtggt tgaccgcctt
     2941 gcagataagc gttcgccagg ttgagctgca acaccggatt ggtgcgtaaa tcgcgggcat
     3001 ttttcaggcg attgattgcc tcattggctt tgttttgccc aagatcgata tcggtagcca
     3061 gatcgagata ccatgcgttg ccaggttctg ccgccagtaa cggttgcagc gttttacgcg
     3121 cctcgtcgta tttattggct tccatcgcct gtaaagcacg accatattgc gccgctcgtt
     3181 gctgacgaac gtttcctttc gcccattcat ccagcaaatc actggtgagc tggttacgtc
     3241 cggaattata catccccagt gtgcgcgctt tcgccagata gaaatcttcc gacgactgca
     3301 ccaccatcgg gcgcatctga ttagcacggt tgcgggcatc tgccagacga ctttccggca
     3361 acgggtgagt caataaaatt tcc
//
LOCUS       DAJTUN010000224         3328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTUN010000224 DAJTUN010000000
VERSION     DAJTUN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.228"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(72..794)
                     /gene="glnQ"
                     /locus_tag="OO470_003896"
     CDS             complement(72..794)
                     /gene="glnQ"
                     /locus_tag="OO470_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6252963.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(791..1450)
                     /gene="glnP"
                     /locus_tag="OO470_003897"
     CDS             complement(791..1450)
                     /gene="glnP"
                     /locus_tag="OO470_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6252964.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(1589..2335)
                     /gene="glnH"
                     /locus_tag="OO470_003898"
     CDS             complement(1589..2335)
                     /gene="glnH"
                     /locus_tag="OO470_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6252965.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            complement(2739..3242)
                     /gene="dps"
                     /locus_tag="OO470_003899"
                     /gene_synonym="pexB"
     CDS             complement(2739..3242)
                     /gene="dps"
                     /locus_tag="OO470_003899"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6252966.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT          812 a          851 c          813 g          852 t
ORIGIN      
        1 catgcaattt taataagttg ccagatatcc tgtaggcctg ataagcgtag cgcatcaggc
       61 aatgtgtctt attaagagac gtgctgcaaa aattcctgca agcgctggct cggcgggttc
      121 ttgatcaaca cctgcggatt gccatcttcc gcaatccggc ctttgtcgat aaagatcagc
      181 cgcgaagcta ctttctcggc aaaaccgatt tcgtgggtca cgatcaccat cgtcatccct
      241 tcttcagcca gatcctgcat aaccttcagc acttcatggc gcagttccgg gtcaagagcg
      301 gaagtcggtt catcaaacag catcattttc ggcttcaccg ccagcgcgcg ggcaatcgcc
      361 acacgctgct gttgaccacc agaaagttcg gaagggtagt gatgtgcacg ttctgccaga
      421 ccgactttcg ccagcagctc acgcgccagt ttttccgcct cttctttgtt cgcgccacgc
      481 acgcgtagcg gaccaaacat gacgtttccc agcgccgtca gatgcgggaa gaggtaaaac
      541 tgctggaaca ccatacctgc ttcctggcga atcaggcgct cgtcaacttt cggatcgttg
      601 accttcaggc catcgacaat cagatcgccg gaggtgattt cttccagttt gttgatgcag
      661 cgcagcaggg tcgatttacc ggaaccggac ggcccgataa tcaccacgac ttcgccctgg
      721 gcaatgttca aatcgatatt gtgcagcacc tgggttgggc caaagtgctt ggagacgttt
      781 ttaaattcaa tcacaggatt ttcatccttc tttccagacg acgcagaata aagctcagca
      841 ccagggtaat aatcagatag aacaccgcca cggcgctcca gatctcaagg gcgcggaagt
      901 taccggcaat aatttcttgc ccctgacggg tcagttccgc cacgccgata acaataaaca
      961 gcgaggtgtc tttaatgctg atgatccact ggttacccag cggcggcagc atacgacgca
     1021 gtgccagcgg taaaatgacg tagcgaatgg tttcccaacg tgaaagaccg agcgccagtc
     1081 ctgcttcacg aaaacctttg tggatagaca gcaccgcacc acgcgtgatt tccgcaatgt
     1141 acgcgccgga gttgatcatg atggtgacca ccgccgcagt aaatgggtcg atgcgtaagt
     1201 cgttaaacgc catcggcagg gcgaaataga taaacatcac ctggacgacg ataggtgtgc
     1261 cgcggatcac ttcaataaag accagcgcga cgtggttggc tatccaacca ccgaaggtgc
     1321 gtgcaaaacc tgccagcaat ccgattacca gaccgccagc cagaccgagg accgaaatcc
     1381 acagggtcat tttggcacct tcaatcagaa gcggaatggc aggccagatg gcactccagt
     1441 caaactgcat atgttgttcc tgttaccgtg gtgtaaataa tcaaattcag ggtaattaat
     1501 ggccccggag cgggtttgcg cccctcaccc taatcctctc cccagggggg agagggaact
     1561 gccagtgcgc tttacaggtg tagcgttatt atttcggttc agtactgaac cattttttgt
     1621 agatttcgtt gtaagttccg ttctcgcgca gggttttcaa cgcgccgttg actttgtcac
     1681 gcagctcgtc gctacctttc gggaacgcaa taccgtattg ctgcgcttcc agagagtcac
     1741 ctaccgcttt gaactgaccg ttaccggcgg ttttgatgaa gtacagaatg tttggcgtat
     1801 cgtgcagaac ggcgtctgcg cggttggtgc ccagttccat ataggcgtta tcgatgttcg
     1861 ggaactgacg cagatcttta gttttgatgt ttgctttcgc gtaatcaacg gagccagtac
     1921 cgctcttcac agcaaccact ttcccgtcga gatctttcac gcttttcaca tcgttattgt
     1981 tagctttcac catcactaac aggccgcttt tgtagtagcc gtcagagaaa tcgatcgctt
     2041 ttttacgctc gtcggtgatg gtaatgcccg ccagcgccag atcgacgttt ttggtttgca
     2101 gtgccggaat gatcccactg aaatccatcg gcttcagttc gtaatccagc ttcagctctt
     2161 tagcgatggc agcccacaga tcaacgtcaa agcccacata tttatcgccc tgtttaaatt
     2221 caaacggaac gaaggcggta tccgtcgcga caactaattt tttatccgcg gcatgagaag
     2281 aaaccgcaaa agccagggtc agtgcagcca gtgaaacttt taatacagac ttcatagcat
     2341 ttcctttttt ctatccacgg gacgatcccc tgcgtgacat tcacatatat gaaaaaatcg
     2401 tgccaatttt gcaactcatt gatttttcga gatgcggcaa aaacgtgatg cacgatttat
     2461 agggcaatac cctgaagatg caccattctg gggcaccaat ctggtgcgct aaaattgtgc
     2521 actcaacaca gtgattattt agcgtaaaac ctgatgaaaa aacaatcttt atgtaacgat
     2581 tgtgtgatga tgtggataca aaaaatttac ttaatcagct ggggatagca gatggatgca
     2641 ctaaataagt gcgttgaggt gggctgcaaa acaaaacggc ctcctgtcag gaagccgctt
     2701 ttatcgggta ctaaagttct gcaccatcag cgatggattt attcgatgtt agactcgata
     2761 aaccacagga atttatccag gtcgcgagac gcggcggtca ggatatctgc ggtgtcgtca
     2821 tctttcgctt cgccaatcgc tttgcgtacg tcattagcga cgattgcgta acggtcagcc
     2881 agttctttca ggtgatcctg aacgttgtgg atgtccagcg ggtaactttt cagcggggtt
     2941 ttgctgttga taacttgagt ggtccccaga gctacaccgc ccagctgcac ggcacgttct
     3001 gccatggtat ccagatgatc gatcagtgcg gtgcggaagc catccagcat ttcatgtacg
     3061 gcaatgaagt tagcgccgcg catgttccag tgcgcttgtt tggtaatcaa agaaagatca
     3121 ataaactgga taacctggcg attcagcaac tctactgttg cttttttctc gctgtcggag
     3181 acatcgttgc gggtataaag cagattggtc gcttttgatt taactaattt agcggtactc
     3241 ataatttcat atcctcttga tgttatgtcc cagtaattaa cgagactaag tatagcaccg
     3301 gctatgtgtt ccgctattct ggctgttc
//
LOCUS       DAJTUN010000225         3310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTUN010000225 DAJTUN010000000
VERSION     DAJTUN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.229"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..608
                     /locus_tag="OO470_003900"
     CDS             <1..608
                     /locus_tag="OO470_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6252967.1"
                     /translation="PAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPP
                     KEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATA
                     ATSKPVTSVASGPRALSRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKP
                     ANMFEREVKNAMRRWRYEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(648..1046)
                     /gene="yciA"
                     /locus_tag="OO470_003901"
     CDS             complement(648..1046)
                     /gene="yciA"
                     /locus_tag="OO470_003901"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6252968.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1151..1690)
                     /locus_tag="OO470_003902"
     CDS             complement(1151..1690)
                     /locus_tag="OO470_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6252969.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(1720..2463)
                     /locus_tag="OO470_003903"
     CDS             complement(1720..2463)
                     /locus_tag="OO470_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6252970.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(2491..2580)
                     /locus_tag="OO470_003904"
                     /pseudo
     CDS             complement(2491..2580)
                     /locus_tag="OO470_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2820..>3310
                     /gene="ompW"
                     /locus_tag="OO470_003905"
     CDS             2820..>3310
                     /gene="ompW"
                     /locus_tag="OO470_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6252971.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYL"
BASE COUNT          945 a          809 c          813 g          743 t
ORIGIN      
        1 tacctgcgcc tgcgcaacca atttctgtca cgatggttgc gcctgctgat ctcgaaccgc
       61 cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa cctgagccga
      121 tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg aaaccgaagc
      181 caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc aaacccgtag
      241 agtcgcgtcc ggcatcaccg tttgaaaata cggcacccgc gcgccccaca tcaagtacgg
      301 caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc gcattaagcc
      361 gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg caggttaaag
      421 ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc tcagccaagc
      481 ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt tatgagccgg
      541 gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc accaccgaaa
      601 ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgactatta ctcaacaggt
      661 aaggcgcgag gttttccttc aggatcaacc gctacatact taaataatgc ttctgtcgct
      721 ttatagcgtt gcccgattgg ttcagacgct acttttttca cccatacttc aatattaatg
      781 ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacaccac atcgccgacc
      841 gcaaccggcc gtaagaaagt cattccttca acccgcacag tcactacgcg accgtgggca
      901 atttcttttg ccagaatagc gccgccaata tccatttgtg acattaacca accaccaaag
      961 atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac aagatcgccc
     1021 tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa tcacaggcgc
     1081 gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc ccgaaggact
     1141 ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag ataccgctta
     1201 acaatgtgaa gattaaggta agggcggtca ggccaaagac tttaaagttg acccaaatat
     1261 tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata aagaaaacag
     1321 cccaggccag atttagcttc gaccataccg gttgcggcag cgtgagttct ttactcagca
     1381 tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct cccgcaaaca
     1441 gagcataaat gactgtcacc ttccatttaa taaactcatc attgtggaag aacagcgtca
     1501 agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca accttacgaa
     1561 agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgct gcagtagccg
     1621 catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa aaatcaagaa
     1681 actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa tcaacatata
     1741 caggcggaac agatagatga gcaatatggc agaaatcagg ttgctcaagg tgttcgccag
     1801 tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca gcaacagtgt
     1861 ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg cccaagtcag
     1921 ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca gcatcaccgg
     1981 tgccagggcc agtaaaatgg ccatgataat tcccggaacg accaccagca tgatgccaat
     2041 ctgtactaaa agggtagtca gaaaaatcag aataaataac ttgggcaata tcggcgcact
     2101 ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct ggataattaa
     2161 tattacgcct ccggcgagaa tggcgttacc gattaattct gaaaaagtgg acgccgctga
     2221 agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt cgaacaaccc
     2281 actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat cactgggtga
     2341 gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca acagaatggt
     2401 cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg ccgtgataga
     2461 catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta ccctggttaa
     2521 gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa gcggaggtaa
     2581 ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa tatttgcgta
     2641 gagaaaatat ttaaaaattg atttaaatca cattaaccag gattctcaat gcaacttcta
     2701 aattaatcca gatcaataaa tggtgaatta tcatatgtaa tgtgatctat gtaggatcat
     2761 ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac ggagcggata
     2821 tgaaaaagtt aacagtggcg gctttggcag taacaactct tctttctggc agtgcctttg
     2881 cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt ccaacagaag
     2941 gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac actcaactgg
     3001 gcttgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg gcagcgacgc
     3061 cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt caccatctgc
     3121 cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc cgtccttacg
     3181 ttggggccgg tattaactac accaccttct ttgataatgg atttaacgat catggcaaag
     3241 aggcggggct ttccgatctc agtctgaaag attcctgggg agctgccggg caggtggggg
     3301 ttgattatct
//
LOCUS       DAJTUN010000226         3293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTUN010000226 DAJTUN010000000
VERSION     DAJTUN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.230"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            144..2060
                     /gene="dnaK"
                     /locus_tag="OO470_003906"
     CDS             144..2060
                     /gene="dnaK"
                     /locus_tag="OO470_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6252972.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2149..3279
                     /gene="dnaJ"
                     /locus_tag="OO470_003907"
     CDS             2149..3279
                     /gene="dnaJ"
                     /locus_tag="OO470_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6252973.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT          895 a          813 c          906 g          679 t
ORIGIN      
        1 acgtgggtta cgaccccatt tagtagtcaa ccgcagtgag tgagtctgca aaaaaatgaa
       61 attgggcagt tgaaaccaga cgtttcgccc ctattacaga ctcacaacca catgatgacc
      121 gaatatatag tggagacgtt tagatgggta aaataattgg tatcgacctg ggtactacca
      181 actcttgtgt agcgattatg gatggcacca ttcctcgcgt gctggagaac gccgaaggcg
      241 atcgcaccac gccttctatc attgcctata cccaggatgg tgaaactctg gttggtcagc
      301 cggctaaacg tcaggcagtg acgaacccgc aaaacaccct gtttgcgatt aaacgcctga
      361 ttggccgccg cttccaggac gaagaagtac agcgtgatgt ttccatcatg ccgttcaaaa
      421 ttattgctgc tgataacggc gacgcatggg tcgaagttaa aggccagaaa atggcaccgc
      481 cgcagatttc tgctgaagtg ctgaaaaaaa tgaagaaaac cgctgaagat tacctgggtg
      541 aaccggtaac tgaagctgtt atcaccgtac cggcatactt taacgatgct cagcgtcagg
      601 caaccaaaga cgcaggccgt atcgctggtc tggaagtaaa acgtatcatc aacgaaccga
      661 ccgcagctgc gttggcttac ggtctggaca aaggcactgg caaccgtact atcgcggttt
      721 atgacctggg gggtggtact ttcgatattt ctattatcga aatcgacgaa gttgacggcg
      781 aaaaaacctt cgaagttctg gcaaccaacg gtgataccca cctgggtggt gaagacttcg
      841 acagccgtct gatcaactat ctggttgaag aattcaagaa agatcagggc attgacctgc
      901 gcaacgatcc gctggcaatg cagcgcctga aagaagcggc agaaaaagcg aaaatcgaac
      961 tgtcttccgc tcagcagacc gacgttaacc tgccatacat cactgcagac gcgaccggtc
     1021 cgaaacacat gaacatcaaa gtgactcgtg cgaaactgga aagcctggtt gaagatctgg
     1081 taaaccgttc cattgagccg ctgaaagttg cactgcagga tgctggcctg tccgtatctg
     1141 atatcgacga cgttatcctc gttggtggtc agactcgtat gccaatggtt cagaagaaag
     1201 ttgctgagtt ctttggtaaa gagccgcgta aagacgttaa cccggacgaa gctgtagcaa
     1261 tcggtgctgc tgttcagggt ggtgttctga ctggcgacgt aaaagacgta ctgctgctgg
     1321 acgttacccc gctgtctctg ggtatcgaaa ccatgggcgg tgtgatgacg acgctgatcg
     1381 cgaaaaacac cactatcccg accaagcaca gccaggtgtt ctctaccgct gaagacaacc
     1441 agtctgcggt aaccatccat gtgctgcagg gtgaacgtaa acgtgcggct gataacaaat
     1501 ctctgggtca gttcaaccta gatggtatca acccggcacc gcgcggcatg ccgcagatcg
     1561 aagttacctt cgatatcgat gctgacggta tcctgcacgt ttccgcgaaa gataaaaaca
     1621 gcggtaaaga gcagaagatc accatcaagg cttcttctgg tctgaacgaa gatgaaatcc
     1681 agaaaatggt acgcgacgca gaagctaacg ccgaagctga ccgtaagttt gaagagctgg
     1741 tacagactcg caaccagggc gaccatctgc tgcacagcac ccgtaagcag gttgaagaag
     1801 caggcgacaa actgccggct gacgacaaaa ctgctatcga gtctgcgctg actgcactgg
     1861 aaactgctct gaaaggtgaa gacaaagccg ctatcgaagc gaaaatgcag gaactggcac
     1921 aggtttccca gaaactgatg gaaatcgccc agcagcaaca tgcccagcag cagactgccg
     1981 gtgctgatgc ttctgcaaac aacgcgaaag atgacgatgt tgtcgacgct gaatttgaag
     2041 aagtcaaaga caaaaaataa tcgccctata aacgggtaat tatactgaca cgggcgaagg
     2101 ggaatttcct ctccgcccgt gcattcatct aggggcaatt taaaaaagat ggctaagcaa
     2161 gattattacg agattttagg cgtttccaaa acagcggaag agcgtgaaat cagaaaggcc
     2221 tacaaacgcc tggccatgaa ataccacccg gaccgtaacc agggtgacaa agaggccgag
     2281 gcgaaattta aagagatcaa ggaagcttat gaagttctga ccgactcgca aaaacgtgcg
     2341 gcatacgatc agtatggtca tgctgcgttt gagcaaggag gcatgggcgg cggtggtttt
     2401 ggcggcggcg cagacttcag cgatattttt ggtgacgttt tcggcgatat ttttggcggc
     2461 ggacgtggtc gtcaacgtgc ggcgcgcggt gctgatttac gctataacat ggagctcacc
     2521 ctcgaagaag ctgtacgtgg cgtgaccaaa gagatccgca ttccgactct ggaagagtgt
     2581 gacgtttgcc acggtagcgg tgcaaaacca ggtacacagc cgcagacctg tccgacctgt
     2641 catggttctg gtcaggtgca gatgcgccag ggattcttcg ctgtacagca gacctgtcca
     2701 cactgtcagg gccgcggtac gctgatcaaa gatccgtgca acaaatgtca tggtcatggt
     2761 cgtgttgagc gcagcaaaac gctgtccgtt aaaatcccgg caggggtgga cactggagac
     2821 cgcatccgtc ttgcgggcga aggtgaagcg ggcgagcatg gcgcaccggc aggcgatctg
     2881 tacgttcagg ttcaggttaa acagcacccg attttcgagc gtgaaggcaa caacctgtat
     2941 tgcgaagtcc cgatcaactt cgctatggcg gcgctgggtg gcgaaatcga agtaccgacc
     3001 cttgatggtc gcgtcaaact gaaagtgcct ggcgaaaccc agaccggtaa gctattccgt
     3061 atgcgcggta aaggcgtcaa gtctgtccgc ggtggcgcac agggtgattt gctgtgccgc
     3121 gttgtcgtcg aaacaccggt aggcctgaac gaaaagcaga aacagctgct gcaagagctg
     3181 caagaaagct tcggtggccc aaccggcgag cacaacagcc cgcgctcaaa gagcttcttt
     3241 gatggtgtga agaagttttt tgacgacctg acccgctaac ctccccaaaa gcc
//
LOCUS       DAJTUN010000227         3105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTUN010000227 DAJTUN010000000
VERSION     DAJTUN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.231"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1622
                     /gene="mscM"
                     /locus_tag="OO470_003908"
     CDS             <1..1622
                     /gene="mscM"
                     /locus_tag="OO470_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6252974.1"
                     /translation="LLWVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIM
                     ALMMFDNLDDREFSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHM
                     LWNMMIGAPLVAILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRR
                     LAFDRAKHRRAEMLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSIL
                     MLIALLSVIVLWSEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIIT
                     TQLVRNLPALLELAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQW
                     LVAALGVGLGFGLQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTIS
                     DWDRKEIIVPNKAFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCS
                     LVIDNPAPEVFLVDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPF
                     PPFQMRLESLNGKQTGRTLTSAGKGRQAGSL"
     gene            complement(1773..3105)
                     /gene="yjeM"
                     /locus_tag="OO470_003909"
                     /pseudo
     CDS             complement(1773..3105)
                     /gene="yjeM"
                     /locus_tag="OO470_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
BASE COUNT          782 a          831 c          777 g          715 t
ORIGIN      
        1 cgctgctgtg ggtagtgatg atttgcgcca cctttgcccg cccgaacggc ttgtttatcg
       61 ctcattttgg ctggccgcgc gaacgtgttt cccgtgggat gcgctactac ctgatgagca
      121 tcgggctgat tatggcgctg atgatgttcg ataacctcga cgaccgcgaa ttttccggtt
      181 cgctgggacg gctttgcttt atcctcattt gcggtgcgct ggcggtggtc accctaagcc
      241 tgaaaaaagc cggtattccg ttgtatctca acaaagaagg cagcggcgac aacattacca
      301 accatatgct gtggaacatg atgattggcg caccactggt tgccattctg gcgtcggcgg
      361 tgggttatct ggcaacggcg caggcactgt tagcgaggct tgaaacctcg gttgccatct
      421 ggttcctgct actggtggtt tatcacgtta tccgccgctg gatgctgatc cagcgtcgca
      481 ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat gttagcgcaa cgtgcgcgtg
      541 gcgaagagga agcgcatcat cacagtagcc cggaaggagc aattgaagtc gatgaaagcg
      601 aagtcgatct cgatgccatc agtgcgcaat ccttgcggct ggtgcgctca attttgatgt
      661 tgatcgccct gctttcggtc attgtgctgt ggtcagaaat ccattccgct ttcggcttcc
      721 tcgaaaatat ttcgctgtgg gatgtcacct ccacggtaca gggcgtagaa agtctggagc
      781 caattaccct cggtgcggtg ctgattgcca ttctggtatt tatcatcacc acgcagctgg
      841 tgcgcaacct gcccgcgctg ctggaactgg cgattttgca gcacctggat ttaacgccgg
      901 gtacgggtta cgccatcacc accatcacaa aatatctgct gatgctgatt ggcgggctgg
      961 tcggcttctc aatgattggt attgagtggt cgaaattgca gtggctggtt gccgcgctcg
     1021 gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa ctttatctct ggcctgatca
     1081 tcctgttcga aaaaccgatt cgcattggcg atacggtaac gatccgcgat cttactggta
     1141 gcgtgacaaa aattaacacc cgcgccacca ccatcagcga ctgggaccgt aaagagataa
     1201 tcgtgccgaa caaggcgttt attaccgagc agtttatcaa ctggtcgctc tctgactcgg
     1261 tcacgcgcgt agtgttgacg attccggccc ctgccgatgc caacagtgaa gaagtgacgg
     1321 aaatcctgct caccgcagcg cgtcgctgct cgctggtgat cgacaacccg gcaccggaag
     1381 tcttcctggt ggatctgcaa caggggattc agattttcga gctgcgtatt tacgccgctg
     1441 agatgggtca ccgtatgccg ctacgccatg agatccacca gctgattctg gcaggcttcc
     1501 acgcccacgg tatcgatatg ccattcccgc ccttccagat gcgtctggaa agcctcaacg
     1561 gtaaacaaac ggggagaacg ctgacgtctg cgggcaaagg tcgtcaggcg ggaagtttgt
     1621 aatgcatgaa gctgccaaat gatgtcaacg tggctcattt agcagcttaa catcatcttc
     1681 agttatcacc ggccctggag agtatcatcc atacgcatta cttcctgaca actcatcgcc
     1741 ggagagacga tgagttgcag gatttcatat gatcagtcga gagctaactc aggtttattc
     1801 gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa gaagacaggg
     1861 ccgccaatca tccacaatgt gctgtcccag tctccggctt caaccacagg ctgaataatg
     1921 gtgaagacgt tcgcaaatgt caccaccaga acgacaacca ccgtcgcaat cattgccgac
     1981 atacgcgttt tgaaaatcac aaatggtctg tcgagatcct gacgtgcttt aaaaaacggg
     2041 aaagccagcg cgaggaacag gtaaggaagc gtcatagaca cgttcgccat cagcgtcagc
     2101 ttgttaaaga acgccgatgc ggtaccgcca ccaaacgaaa ccagcagaat gaagacagta
     2161 accaacccgc actgcatcca catggcgata gacggcatcc ccatcgcatt caggcgcgtc
     2221 atcggttccg gccacaacgc tttcggcgtc ccctggatga tggctttcag cggtgaatag
     2281 caaacgcacc ggtataagcg aggaacatcg aaagtccggt aatacgcgca aaccatacgc
     2341 ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt acccagcgtc accccgaggc
     2401 tcttcatcag cacataggta atattgccaa ggttaacgga accattactt aatacctgct
     2461 gccagtttgt gctgacgccc cataaaaata ttgccagcga ataaccgatt gaaataacaa
     2521 tagcggcaaa aacaatacct ttggcaaagt tcttttctgg attttccgtt ttatcgacca
     2581 gaccaccaac cgcttcaatt ccgccatagg caaaaatagc aaataccaca aacgatagca
     2641 ttgccagacc ggactgataa cccgggttcg gtgatgcaag gaaattaata tcctgcgcga
     2701 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
     2761 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc ccctttgaag
     2821 caacgacggt gaccagaatc atccatgcta ctgccagcag accaaccacc tgcgtaggct
     2881 ccagtccggc aatacgccag tgctgggtca tgtcactacc atagaggaat gttgagaacg
     2941 gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa
     3001 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac
     3061 cttcttcttt gcgataagca gctcccattt cagccatcat taagg
//
LOCUS       DAJTUN010000228         3027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTUN010000228 DAJTUN010000000
VERSION     DAJTUN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.232"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..1005
                     /gene="nanT"
                     /locus_tag="OO470_003910"
     CDS             <1..1005
                     /gene="nanT"
                     /locus_tag="OO470_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6252975.1"
                     /translation="VYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAG
                     KAPVRTMVDILYRGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFIS
                     FMVQSTGKRWPTGVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGF
                     GAAVGCCVGGFLGDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLG
                     QGIAGILPKLIGGYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASL
                     SFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS
                     "
     gene            1053..1742
                     /locus_tag="OO470_003911"
     CDS             1053..1742
                     /locus_tag="OO470_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6252976.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            1739..2614
                     /gene="nanK"
                     /locus_tag="OO470_003912"
     CDS             1739..2614
                     /gene="nanK"
                     /locus_tag="OO470_003912"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6252977.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            2611..>3027
                     /locus_tag="OO470_003913"
     CDS             2611..>3027
                     /locus_tag="OO470_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6252978.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPG"
BASE COUNT          632 a          782 c          901 g          712 t
ORIGIN      
        1 gtctatagcc tggtggttcc ggtctggggt tggcgtgcgc tgttctttat cggcattttg
       61 ccaatcatct ttgctctctg gctgcgtaaa aacatcccgg aagcggaaga ctggaaagag
      121 aaacacgcag gtaaagcacc agtacgcaca atggtggata ttctctaccg tggtgaacat
      181 cgcattgcca atatcgtaat gacactggcg gcggctactg cgctgtggtt ctgcttcgcc
      241 ggtaacctgc aaaatgctgc aatcgtcgct gttctcgggc tgttatgcgc cgcaatcttt
      301 atcagcttta tggtgcaaag tacgggtaaa cgctggccaa cgggcgtaat gctgatggtg
      361 gtcgtgttgt ttgctttcct ctactcatgg ccgattcagg cgctgctgcc aacgtatctg
      421 aaaaccgatc tggcttataa cccgcatact gtcgccaatg tgctgttctt tagtggcttt
      481 ggcgcggccg tgggatgctg cgtaggtggc ttcctcggtg actggctggg aacccgcaaa
      541 gcgtacgttt gtagcctgct ggcctcgcag ctgctgatta ttccggtatt tgcgattggc
      601 ggcgcaaacg tctgggtgct cggtctgtta ctgttcttcc agcaaatgct tggacaaggg
      661 atcgccggga tcttaccgaa actgattggc ggttatttcg ataccgacca gcgtgcagcg
      721 ggcctgggct ttacctacaa cgttggcgca ttgggtggcg cactggcccc aatcatcggc
      781 gcgttgatcg ctcaacgtct ggatctgggt actgcgctgg catcgctctc gttcagcctg
      841 acatttgtgg tgatcctgct gattggtctg gatatgcctt cccgcgttca gcgttggctg
      901 cgcccggaag cattgcgtac tcatgacgct atcgacggca aaccatttag cggtgccgtg
      961 ccatttggca gcgccaaaaa cgatttagtc aaaaccaaaa gttaatcctg ttgcccggtc
     1021 tatgtaccgg gcctttcgct aagggaagat gtatgtcgtt acttgcacaa ctggatcaaa
     1081 aaatcgctgc taacggtggc ctgattgtct cctgccagcc ggttcctgac agcccgctcg
     1141 ataaacccga aatcgtcgca gccatggcat tagcggcaga gcaggcgggc gcggttgcca
     1201 ttcgcatcga aggtgtggca aatctgcaag ccacgcgtgc ggtggtgagc gtgccgatta
     1261 ttggaattgt gaaacgcgat ctggaggatt ctccggtacg catcacggcc tatattgaag
     1321 atgtcgatgc gctggcgcag gcgggcgcgg acattatcgc cattgacggc accgaccgcc
     1381 cgcgtccggt gcctgttgaa acgctgctgg cacgtatcca tcatcacggt ttactggcga
     1441 tgaccgactg ctcaacgccg gaagacggcc tggcatgcca aaagctggga gccgaaatta
     1501 ttggcactac gctttctggc tataccacgc ctgaaacgcc agaagagccg gatctggcgc
     1561 tggtgaaaac gttgagcgaa gccggatgtc gggtgattgc cgaagggcgt tacaacacgc
     1621 ctgctcaggc ggcagatgcg atgcgccacg gcgcgtgggc ggtgacggtc ggttctgcca
     1681 ttacgcgtct tgaacacatt tgtcagtggt acaacacagc gatgaaaaag gcggtgctat
     1741 gaccatactg gcgattgata tcggcggtac taaacttgcc gccgcgctga ttggcgctga
     1801 cgggcagatc cgcgatcgtc gtgaacttcc tacgccagcc agccagacac cagaagcctt
     1861 gcgtgatgcc ttatccgcat tagtctctcc gttgcaagct catgcgcagc gggttgccat
     1921 cgcttcaacc ggaattattc gcgacggcag tttgctggcg ctgaatccgc ataatctcgg
     1981 tggattgcta cactttccgt tagtcaaaac gctggaacaa cttaccaatt tgccgaccat
     2041 tgccattaac gacgcgcagg ccgcagcatg ggcagagtat caggcgctgg aaggcgatat
     2101 aaccgatatg gtctttatca ccgtttccac tggcgtcggc ggcggtgtag tgagcggcgg
     2161 caaactgctt accggccctg gcggtctggc ggggcacatc gggcatacgc ttgccgatcc
     2221 acacggccca gtctgcggct gtggacgcac aggttgcgtg gaagcaattg cttctggtcg
     2281 cggcattgcg gcggcagcgc agggcgagct ggctggcgcg gatgcgagaa ctattttcac
     2341 acgcgccggg cagggcgatg agcaggcgca gcaactgatt catcgctccg cacgtacgct
     2401 tgcaaggctg atcgctgata ttaaagccac aactgattgc cagtgcgtgg tggttggtgg
     2461 cagcgttggt ctggcagaag ggtatctggc gttggtggaa atgtatctgg cgcaggagcc
     2521 agcggcattt catgttgatt tactggcggc gcattaccgc catgatgcag gtttacttgg
     2581 ggctgcgctg ttggcccagg gagaaaaatt atgatgatgg gtgaagtaca gtcattaccg
     2641 tctgctgggt tacatcctgc gttacaggac gcgttaacgc tggccttagc tgccagaccg
     2701 caagaaaaag cgccgggtcg ttacgaatta cagggcgaca atatttttat gaatgtcatg
     2761 acgtttaaca ctcaatcgcc cgtcgagaaa aaagcggaat tgcacgagca atacattgat
     2821 atccagctgt tattaaacgg tgaggaacgg attctgtttg gcatggcagg cactgcgcgc
     2881 caatgtgaag agttccacca tgaagatgat taccagcttt gtagcgccat tgaaaacgag
     2941 caaaccatca tcctgaaacc gggaatgttc gccgtgttta tgccaggaga gccgcataag
     3001 ccgggatgcg ttgttggcga gccgggt
//
LOCUS       DAJTUN010000229         2991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTUN010000229 DAJTUN010000000
VERSION     DAJTUN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.233"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(551..745)
                     /locus_tag="OO470_003914"
                     /pseudo
     CDS             complement(551..745)
                     /locus_tag="OO470_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
     gene            1594..2703
                     /gene="fepE"
                     /locus_tag="OO470_003915"
     CDS             1594..2703
                     /gene="fepE"
                     /locus_tag="OO470_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6252979.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          754 a          613 c          711 g          913 t
ORIGIN      
        1 cgaaaggcag gaacaggaga gcgcacgagg gagccaccag ggggaaacgc ctggtatctt
       61 taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      121 gggggcggag cctatggaaa aacgccaccg gcgcggcctt ttttcggggc ctctcttttg
      181 ctcacatgtt ttttctggtg ttatcccctg attctgtgga taaccgtata tccgtctttg
      241 cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      301 aagcggaaga gcgccggaag ctgcattttc tccttacgta tctgtgcagc atttcacacc
      361 tcatatgtcg ctgttctctc accatctgct ctgccgcctc ataattaagc cagtatacac
      421 tccgttatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa aaccccctga
      481 cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg ggaaagccgt gaccgcctcc
      541 gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa gcgaatctgg atgctgttct
      601 ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc cgcctgttcg tcggcatcca
      661 gagcatccag tttttcaagc agtgtcaggc tctggctttt gatacatcgc gccatattca
      721 gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc gtcagttccg ggtgtcgggg
      781 ggggaccctg agcaggtggt aattgtaaga cgttgcgggc gatggtatta caattgcaca
      841 tcctgtcctg tttctttagg acgtatcatg gcacagcata tcaactgata aataattcct
      901 gtgcagtgca gccgctgttg cataaaattt cgctctgcgt cgagtgtttc ttaatcaata
      961 attccgctga tactccggaa aaacgcgagt ctgttctcat gcctgaaatg cccacacccc
     1021 acgcaaaaac aagtttttgc tgatttttct ttataaatag agagttatga caaattagtt
     1081 cttcttgctc tctttgtgat atttaaaaaa gcggtgtcgg cgcggtgttg tagctgcgcc
     1141 aacaccgctt tttaggggtg gtactgacta ttttcataaa aaaaacatca tcttatatta
     1201 ggggtgctgc tagcggcgcg gtgtgttttt ttacaggacg ccctgggggc gctgctaggg
     1261 gtgtctgttc agatatgtag caatgagtca gggattcacg aactgaaaat gtccgaaaat
     1321 gtgtctcatg ggcactgtat gcgcccaaac cgatcgtcat tcactttcat gcatagctat
     1381 gcagtgagct gaaagcgatc ctgacgcatt tttccggttt accccgggga aaacatctct
     1441 ttttgcggtg tttgcggcag aatcgcgttc agcgcgtttt agcggtgcgc gcaataaaat
     1501 gttatgttaa atgtctgctg aatggttttt aggttttttc ctaacatatg atagaataaa
     1561 aaaaccatat gttaatatat tgtgtttttt tcaatgaaaa tctcaaaggt ctctgacgaa
     1621 catttttcta attacaacat gttgccgaat ggtaatgaag aaattgacct ttttggcttg
     1681 attagcgttt tatgggcagc aaaaataaaa attgttgggg tgattctatt ttttgcttgt
     1741 attggctttc ttgtttcttt tgttttgcca caaaaatgga ctagttctgc tgtggttact
     1801 cctgctgagt ctgttcaatg gagtgaatta aataaggagc tttccaagct tcatgttctg
     1861 ggagtgggtt ttgatatcaa tcgtgatagc gcatttgctc tgtttattaa gaaatttcag
     1921 tccgttaatt cgttgaatga gtatatgaga tcatctcctt atgttatgga gattattaaa
     1981 aagaataaca ttagcgctct cgaactgcat cgtgcaattg ttgagttgag taaaaatatg
     2041 aagtcgattg atgataatgc cagtaagaaa aacgacaagt catcattgta tgtatcatgg
     2101 actctgagtt ttactgctcc aacaagtaaa gaagctcacg atgtgttgtc tgggtatatt
     2161 aattatgttt cttcccttgt tgtaagggat ttgatggaag atataagaaa taaactagaa
     2221 gttaaaacta atgttgaaaa agaaattctt gcactggatg agataaaaat tagaaaccag
     2281 ctgaatgcag atattcgacg cctcaattat tcactggagg ttgctaatgc ggctggaata
     2341 aaaaaacctg tatacagcaa tggtcagatt atgaaggatg acccagattt tcctgtggct
     2401 ctcggttctg atggtatagc aactaaattg aacatcaaaa aatcaatcaa ggatgtttcg
     2461 gaattgagtg gggagttgcg aaatcgtcaa tatgttgtga atcaattggt tgtggcgaaa
     2521 gtgggggatg ttgattttat gccatttcag tatcaattga gcccaacgtt accggtcaga
     2581 aaggatgggc caagtaatgc cataattgta attctttctt ctgtcattgg agggatgctt
     2641 gcatgtggct atatactttt acgtcatgcc atggaggcca gaaagatgag tgtgattgag
     2701 taaaagttgt gctgatgcat cattttataa gcaactttta taaaaagttc aaaggatctt
     2761 cttgagatca ttcccttaca gccgtagtct tctactctag aaaacgaaaa aaccacctgg
     2821 ggaggtggtt tttcgaaggt tcagtaagtt ggggaacttc tgaaccgtgg taacagtctt
     2881 gtgcgagaca tgtcaccaaa tctgtccttc tagtgtagcc tcagtaaggc tgccacttca
     2941 agaactctca atacatctct cgcacatcct gtttaccagt ggccgctgcc a
//
LOCUS       DAJTUN010000230         2975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTUN010000230 DAJTUN010000000
VERSION     DAJTUN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.234"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..399)
                     /locus_tag="OO470_003916"
     CDS             complement(<1..399)
                     /locus_tag="OO470_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6252980.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFR"
     gene            complement(446..1165)
                     /gene="yehT"
                     /locus_tag="OO470_003917"
     CDS             complement(446..1165)
                     /gene="yehT"
                     /locus_tag="OO470_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6252981.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1162..2847)
                     /gene="btsS"
                     /locus_tag="OO470_003918"
     CDS             complement(1162..2847)
                     /gene="btsS"
                     /locus_tag="OO470_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6252982.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          765 a          790 c          685 g          735 t
ORIGIN      
        1 gcggaagttt ttatgatccg gtgcgcgcat catcgctgta atttccggca tcgaaacgcg
       61 gaactgctgt tcggtgagga tagccagaat gtcatcggtt ttcagcgaaa acgcgatgcg
      121 taattttttc agcacgatgt tgttattaat gcgacgttcc ggctccagtg ccggagcaga
      181 ctcatccttg ccgcgttttt cataaatcag gccattgagg aatgacgaca aaacaatgtc
      241 cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc cagacggcga tctgttccgc
      301 ggtggcttcg acattatcca gcgccagaat acgcaccagg tcattattat tggctttcaa
      361 aatgtagcgc acgctgcgca gaatatcgtt acttatcatg aggccttcag gtgttgatga
      421 ggcaaaaagc cattttagca gtcttttaca ggccaatcgc ctcttttaag cttttcagat
      481 aacggcggct gaccggcacg gttaagccat tacgcaaaat caactcggcc tggccgttat
      541 cttccagacg aatctcctgt aaatgcgcga ggttaaccag atactgacga tggcagcgca
      601 gtagtggtgt acgactttcc aaggtacgta atgtcaattc ggtaaagccc tctttccctt
      661 cgtggctggt aacgtagaca ccgctcatcc gactgctgac aaatgccaca tctttcattt
      721 gcagcaaata aatccgacta tgccccgtac aagggataaa tttcagcgcc tgttgatttt
      781 ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg caatcgcgcc agcgttttct
      841 ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa ggcatgttct tcaaaggctt
      901 taatagcgta ttcgtcaaac gcagtgagaa aaacaatata cgggcgatgt tccgggtcaa
      961 gcatccccac catttccaga ccactgatgc gcggcatctg gatatcgaga aacagcacat
     1021 ccgggcgcag tttatgcacc gcgccgatcc cttccacggc gtttgaacac tctccaacga
     1081 tttcaatatc gctctgctcc tgcaaaaata tacgcaggtt ctcccgtgct aacggttcat
     1141 catcgacaat taagacttta atcatgcctc gtccctccat ggtagtcgta acgttattcg
     1201 ggtgtaacta tcaggctcac aggcgacgct tattccatag tcatcgccaa accgttcacg
     1261 taaacgctta tccaccagat tcatccccag cccactggca ttggttaccg gttgatacaa
     1321 accggcattg tcttcgatct ccagcatcaa atgttgcccc tcacgtcggg cgctgattgc
     1381 cactcgccct gtatccagca gttgtgatgt cccatgttta atggcgtttt ccactatcgg
     1441 ttgcagggta aacgcgggca attgctgctg ggataattct tgcggaatag caatgttgac
     1501 ctgcaaccgc gactggaagc gcgccttttc aatttgcaga taagcgttca catgttcaat
     1561 ttcgtcggcg agagtaacaa actccgaagg ccgctttaag tttttgcgga aaaaagtgga
     1621 aagatactgc accagctggc tggcctgttc gctgtcgcgg cggatcaccg ctttaatggt
     1681 gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg tgaagcagtt tgatttctga
     1741 ctgggtgagc atcgcttttt gccgctcata ttgcccggca agaatctgcg ccgaaagcag
     1801 ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg aataaccggt ttttggcttc
     1861 atacaatttg atggtgccca tcacgcgctg attttcgcca cgcaacggaa ttaccagcgt
     1921 cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa ggtacttcgt tgccatcagc
     1981 gtagaccact tcaccggttt caatcgcttt taaggtgtaa gtcgaagaaa tcggtttgcc
     2041 gggtaaatgg tggtcgtcac caattccggt aaaggccagc aatttctctc gatcggtaat
     2101 cgcgactgca ccaatatcca gctcctgata cagtacctga gccactttca tgctgttcac
     2161 ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct gccactttca gcgcagtggc
     2221 agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta tcgagcaata tacgcataaa
     2281 cagcgccgcg ccgacggtat tggtgaccat cattggcgca gcaatattac tcaccagacg
     2341 caccgcatct tcataaggtc gggcgatcgc aaggatgatc agcatttgca ccatttcagc
     2401 gacgaacgtg acggcaccgg cggtaatggg gttaaagact ttatcagtgc gcccgcgacg
     2461 gatcaggatg ctgtgtacca ggccgccgag caatccttca acgatggttg agatcatgca
     2521 acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag ccgccggtca gaccaaccag
     2581 cccaccgacg accggaccgc cgagtaagcc gcccattacc gcgcctatcg cacgggtatt
     2641 ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc atgatgcaga agatggaaaa
     2701 gacgatgtag cagagaaatt tatgcggcag acgaaccgtg acctgcatta acggtatgaa
     2761 taatggcgtt ttactcatta accacgcaat gactaaaaaa acgcacatct gctgaagcag
     2821 cagcaacacc agattaaaat cgtacatacc cgcaaaccac acttcccttt aaaacgcgta
     2881 acatacattg cctgcgttta actttctttg aactcttgca gaaaaatgag aattcgtgag
     2941 tacgatcact caaaatcgcc tggcaaaaat aaaat
//
LOCUS       DAJTUN010000231         2972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTUN010000231 DAJTUN010000000
VERSION     DAJTUN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.235"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(133..1332)
                     /locus_tag="OO470_003919"
     CDS             complement(133..1332)
                     /locus_tag="OO470_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6252983.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
     gene            1564..>2972
                     /gene="pitA"
                     /locus_tag="OO470_003920"
     CDS             1564..>2972
                     /gene="pitA"
                     /locus_tag="OO470_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6252984.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTV"
BASE COUNT          691 a          787 c          771 g          723 t
ORIGIN      
        1 aaaataacca caacggcaat agcgttaatt cgctaacaaa acagcgagtt aacaccagaa
       61 tgtcggatgc agaaccgtac caaaatgact taataacgac atatacaaac ggctgacggg
      121 aaaacgtcat ggttatttca caattgacgc caaatcctgc gcacaagccc atgcactcga
      181 ccacgcccac tggaagttat agccccccag ccagccggta acgtccatca cttcgccgat
      241 gaagtacagc ccaggcactt tgcgcgcttc catcgtccgt gaagaaagtt cgttggtgtc
      301 cacgccgccg agcgtcactt cggcagtgcg atagccttca gtgccgttgg gttgtacgcg
      361 ccagtcggtc aatgtgctaa tcagtgcctg ttggtcacac acgttgagct gttttagcga
      421 aacatccggg atttgcccga gttgctgtaa gcgttcaacc aaccgcttcg gtagatgaac
      481 cgccagtgtg tttttcaggc tttgattcgg atgtgcgtta cgctgctcat tcaggaaggt
      541 ttcgaggtcc acatccggta gcagattgat actgacaaat tcacccggtt gccagtagct
      601 tgaaatctgc aacaccgccg gtccagataa gccgcggtgg gtgaagagta agttctcacg
      661 gaaaacggtg ccgttttcag cggtaatcac ggaaggcacc gccacgcccg ccagcacctg
      721 taactcttcg agcaacggtt tatgcagagt gaatggcacc agacccgcgc gggtcggcag
      781 cacgttgagg ccaaattgtt cggcaatctt ataaccaaac ggcgatgcgc ccagccccgg
      841 cattgacagc ccaccggtcg cgatgaccag cttttcgcaa ccgacagtca tgccgttcag
      901 atcaagcgtg aagcctgttt catccttcgc cacactcagc acttcgctac gcaatctgaa
      961 ggttacattg cccttctcgc actcatccac cagcatgtcg acaatctgct gcgcggagtc
     1021 atcgcagaag agttgcccta acgttttctc gtgccaggcg ataccgtgtt tattgaccag
     1081 atcaatgaaa tcccactggg taaaacgtgc gagtgcagac ttacaaaaat gcggattctg
     1141 gctcagataa gcgcctggtt cgacataaag gttggtaaag ttgcagcgcc caccgccaga
     1201 cataaggatt ttgcgcccta gtattttacc attatcgatc agcagaaccc ggcgtcctac
     1261 ctgacctgcc agcgcagaac agaacatacc cgccgcacca gcgcctataa taatggcatc
     1321 aaacctttcc acgttgcgct cctcttagaa aaaacgggcg tgaattgtaa agattcctca
     1381 gtggtcgcac cagcatcaat attactaaaa ggaagtattt gcctgaatta tataagataa
     1441 ttattttttg agtgaaatcc atacaggagg caaatcaaaa aaagtctata tttcactttg
     1501 cccgcgccgc gaaagtcact gataatgcgc cgcgttcatg tcctcaaaat ggcgtaacgt
     1561 cctatgctac atttgtttgc tggcctggat ttgcataccg ggctgttatt attgcttgca
     1621 ctggcttttg tgctgttcta cgaagccatc aatggtttcc atgacacagc caacgccgtg
     1681 gcaaccgtta tctatacccg cgcgatgcgt tctcagctcg ccgtggttat ggcggcggtg
     1741 ttcaactttt tgggtgtttt gctgggtggt ctgagtgttg cctatgccat tgtgcatatg
     1801 ctgccgacgg atctgctgct taatatggga tcgtctcatg gccttgccat ggtgttctct
     1861 atgttgctgg cggcgattat ctggaacctg ggtacctggt actttggttt acctgcatcc
     1921 agctctcata cgctgattgg cgcgatcatc gggattggtt taaccaatgc gttgatgacc
     1981 gggacgtcag tggtggatgc actcaatatc ccgaaagtat taagtatttt cggttctctg
     2041 atcgtttccc ctattgtcgg cctggtgttt gctggcggtc tgattttctt gctgcgtcgc
     2101 tactggagcg gcaccaagaa acgcgcccgt atccacctga ccccagcgga gcgtgaaaag
     2161 aaagacggca agaaaaagcc gccgttctgg acgcgtatcg cgctgatcct ttccgctatc
     2221 ggcgtggcgt tttcgcacgg cgcgaacgat ggtcagaaag gcattggtct ggttatgttg
     2281 gtattgattg gcgtcgcgcc agcaggcttc gtggtgaaca tgaatgccac tggctacgaa
     2341 atcacccgta cccgtgatgc catcaacaac gtcgaagctt actttgagca gcatcctgcg
     2401 ctgctcaaac aggctaccgg tgctgatcag ttagtaccgg ctccggaagc tggcgcaacg
     2461 caacctgcgg agttccactg ccatccgtcg aataccatta acgcgctcaa ccgcctgaaa
     2521 ggtatgttga ccaccgatgt ggaaagctac gacaagctgt cgcttgatca acgtagccag
     2581 atgcgccgca ttatgctgtg cgtttctgac actatcgaca aagtggtgaa gatgcctggt
     2641 gtgagtgctg acgatcagcg cctgttgaag aaactgaagt ccgacatgct tagcaccatc
     2701 gagtatgcgc cggtgtggat catcatggcg gtcgcgctgg cgttaggtat cggtacgatg
     2761 attggctggc gtcgtgtggc aacgactatc ggtgagaaaa tcggtaagaa aggcatgacc
     2821 tacgctcagg ggatgtctgc ccagatgacg gcggcagtgt caatcggcct ggcgagttat
     2881 accgggatgc cggtttccac cactcacgta ctctcctctt ctgtcgcggg gacgatggtg
     2941 gtagatggcg gcggcttaca gcgtaaaacc gt
//
LOCUS       DAJTUN010000232         2783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTUN010000232 DAJTUN010000000
VERSION     DAJTUN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.236"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..717
                     /gene="fecD"
                     /locus_tag="OO470_003921"
     CDS             <1..717
                     /gene="fecD"
                     /locus_tag="OO470_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6252985.1"
                     /translation="ASPDILGVNHAASLASVGALLLMPSLPVMVLPLLAFAGGMAGLI
                     LLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSRPQDVNNALLWLTGSLWGRDWSF
                     VKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLGVSVPHTRFWALLLAVAMTSTGV
                     AACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTGALLLVVADLLARIIHPPLELPV
                     GVLTAIIGAPWFVWLLVRMR"
     gene            718..1485
                     /gene="fecE"
                     /locus_tag="OO470_003922"
     CDS             718..1485
                     /gene="fecE"
                     /locus_tag="OO470_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6252986.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2043..2300)
                     /locus_tag="OO470_003923"
     CDS             complement(2043..2300)
                     /locus_tag="OO470_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6252987.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(2383..2783)
                     /locus_tag="OO470_003924"
                     /pseudo
     CDS             complement(2383..2783)
                     /locus_tag="OO470_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          604 a          745 c          740 g          694 t
ORIGIN      
        1 gcatcaccgg atattctcgg cgttaaccat gccgccagcc tggcctctgt gggggctcta
       61 cttcttatgc cgtcactgcc cgtgatggtg ctgccgctgc tggcctttgc gggcggcatg
      121 gcggggttga tattactgaa gatgctggca aagacccacc agccgatgaa gctggcgctc
      181 accggcgtgg cgctttctgc atgctgggcc agcctgacgg attatctgat gctctcgcgc
      241 ccgcaggatg tgaacaacgc cctgctgtgg ctgaccggca gcttatgggg ccgtgactgg
      301 agctttgtga agattgccat cccgctgatg attttatttc tgccgctgag cctgagtttt
      361 tgccgcgatc tcgacctcct tgcactcggc gatgcgcgcg ccaccacgct cggtgtgtcg
      421 gtgccccata cccgattctg ggctttgtta ctagctgtcg ccatgacatc taccggcgtg
      481 gccgcctgcg gcccgattag ctttattggt ctcgtggtgc cgcatatgat gcgtagcatc
      541 accggtggac gtcaccgcag actgctgcct gtttcagccc tgacaggtgc gttgctgttg
      601 gtggttgccg atctgctggc gagaattatt catcccccac tggagctccc ggttggcgtg
      661 ctgaccgcca ttatcggtgc gccgtggttt gtctggttgc ttgtgagaat gcgataaatg
      721 actttacgaa ctgaaaatct gacggtcagt tacgggacag acaaggtact taacgacgtt
      781 tcactctcac tgccaacggg gaagatcacc gccctgatcg gccctaacgg ttgcgggaaa
      841 tcgacgctgt taaactgttt ttcgcggctt ttaatgccgc agtctggcac cgtatttctc
      901 ggcgataatc ccataaatat gctctcatca cgccagttgg cccgcaggct ttcgctgctg
      961 cctcagcacc atttaacgcc agaggggatc acagtccagg agctggtttc gtatggtcgt
     1021 aatccctggc tgtcactctg ggggcgtctc tccgctgaag acaatgcacg agttaatgtc
     1081 gccatgaacc agacccggat caatcatctt gccgttcgtc ggttaaccga gctttccggc
     1141 ggtcagcgcc agcgcgcatt tctggcgatg gtcctggccc agaatacgcc cgttgtatta
     1201 cttgatgagc caaccaccta tcttgatatc aatcaccagg tggacctgat gcggttgatg
     1261 ggcgaactcc ggactcaggg gaaaacggtg gtcgctgtgc tgcacgacct taatcaggct
     1321 agccggtact gcgatcaact ggtggtaatg gcaaacggac atgttatggc gcaaggcaca
     1381 ccagaagagg tgatgacccc aggattgctg agaacagtat tcagcgtgga agcggaaata
     1441 caccccgagc cggtatctgg caggccgatg tgcctaatga ggtagattgc acaggccgta
     1501 agaaccaaac cacgactgaa tgaaactgga ctggcgccag caagcctgtt cagactgggg
     1561 ctgaactttt ccggactctg aaagattacc aatactcatc gtccatccgc ttgctttagg
     1621 ctgacaggtt cataatcaac gcaaaccaga gctgtacagg cttgggcgcg gctttcaaac
     1681 cagtcgtgat cacggcaatc aattttgaac tctgcttaac ggacatttct gtataaccct
     1741 tacggcaacg aaaaacgcga agttaaaatt ttagaaaccc aaaaacgtga catgactaag
     1801 tttagatttc agggggggag atcaaaaaat ttcgctctgt gccagagcgg acattcacgg
     1861 agctggttca ttaccaatga ggttgggctt ttgaggataa atcaatgatc agacgccaac
     1921 gtaaatcaaa agcaaccctg gaacggaatt gctaatccag tttctgacca tcgatttttc
     1981 taaaaagtgt gcgtttgcta ctacttaggt aggtgcagct ttcttaatca ccggcagcca
     2041 cgttatacag gccagttgat ggatcgattg ttatcaatga tatcttcatg agtcggtgtc
     2101 tcacccagct taccgaagct ggcataaagt gaaggcagac gggcccgtcc ttctcccttt
     2161 ttcgccagag ggaaagcgcg aagcatggtg gcagcctccc agtcacaaca ataggatggt
     2221 gtgcacggct gctgacgcca tgattcagcg atagagccgg aaaatacggg gtcaaagccg
     2281 gtatcattaa ccagagtcat cgtgacctgt actgcggctg gatgatctcc tgctactgca
     2341 actgctaggc gaccgcggct cccttcaggt agtctgttgt ggtcaactaa aactggcctc
     2401 cgcgttagag tttttccagt atcggttttc tgattcgttt ggtggtaacc caccattata
     2461 ttcgtgcggt cttagtgcgc tgtaatatcc aacgatatag tccgttattg cgtgagctgc
     2521 atcgctgaag cttacatagc ccgtcgctgg cacccattag ttcttcagac tcctgaagaa
     2581 gcgctccatt gggctgttat cccagcagtt tccacgccgg ctcatactct gtctgatctg
     2641 gtatcgccac agtaactgcc ggaactgcct gctcgtataa tgactgcctt gatcgcttgg
     2701 aacatcaccc cgacgggctt accacgggtt tcccatgcca tttccagtgc tttcatggta
     2761 agcctgctgt ccggcgagaa cga
//
LOCUS       DAJTUN010000233         2697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTUN010000233 DAJTUN010000000
VERSION     DAJTUN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.237"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="OO470_003925"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="OO470_003925"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6252988.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2117)
                     /locus_tag="OO470_003926"
     CDS             complement(1725..2117)
                     /locus_tag="OO470_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6252989.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2478)
                     /locus_tag="OO470_003927"
     CDS             complement(2119..2478)
                     /locus_tag="OO470_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6252990.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
BASE COUNT          666 a          649 c          674 g          708 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctggtccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DAJTUN010000234         2690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTUN010000234 DAJTUN010000000
VERSION     DAJTUN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.238"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            230..769
                     /locus_tag="OO470_003928"
     CDS             230..769
                     /locus_tag="OO470_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6252991.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OO470_003929"
     CDS             complement(995..1318)
                     /locus_tag="OO470_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6252992.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OO470_003930"
     CDS             1604..1798
                     /locus_tag="OO470_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6252993.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJTUN010000235         2594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTUN010000235 DAJTUN010000000
VERSION     DAJTUN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.241"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            272..706
                     /gene="slyA"
                     /locus_tag="OO470_003931"
     CDS             272..706
                     /gene="slyA"
                     /locus_tag="OO470_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6252994.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(753..1220)
                     /gene="slyB"
                     /locus_tag="OO470_003932"
     CDS             complement(753..1220)
                     /gene="slyB"
                     /locus_tag="OO470_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6252995.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1494..>2594
                     /gene="anmK"
                     /locus_tag="OO470_003933"
     CDS             1494..>2594
                     /gene="anmK"
                     /locus_tag="OO470_003933"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6252996.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPA"
BASE COUNT          697 a          638 c          634 g          625 t
ORIGIN      
        1 ggaaaataga cggacgcacc gaacaccaga tcttgtaagg gcaatcctgt tgcattgagc
       61 ataaacttca caaagtagat tcctttacga ccgaatatat tgcgttagat taataaatat
      121 tctttaagtg cgaaaaattt acgcgcaatt tctgaaaaat atgtctgacg gtaaccaaat
      181 gcagcaatac atttgtttta gcaatacaat tgctgcacac tattctaaaa gccgcataat
      241 atcttagcaa gctaattata aggagatgaa attggaatcg ccactaggtt ctgatctggc
      301 acggttggtg cgcatatggc gtgctctgat agaccatcgc ctgaaaccgc tggagttaac
      361 acaaacccat tgggttacgt tacacaatat ccatcagtta cctccagacc agtcgcaaat
      421 tcaactggca aaagcgattg gcatcgagca gccatcactg gtccgtactc tggaccaact
      481 ggaagaaaaa gggttaattt cgcgtcaaac ttgtgccagc gatcgtcggg ctaaacgtat
      541 taaactgacg gaaaaggcag agccactgat cagcgaaatg gaagctgtta ttaacaaaac
      601 ccgcgcggaa atattacatg gcatctccgc agaggaactg gagcaactga ttacgctcat
      661 cgcaaaactt gagcataata tcattgagtt acaggccaaa gggtgaaatg aagggggcgt
      721 gtggtcagta acctgaccac acgcaaactt atttagcgcg gagaaacggt cacctgactg
      781 ccattgctgg ccagtacgac acgttggccc ggagagaaac gagtgttgcc ttgtttctgt
      841 accaccatga tggtattacc atcgtcttta cgaatttcca gctcgacacc ctgcgttttg
      901 ttcattgcac tctgtacgcc ctgaccagct acgccacctg caacagcgcc tgctgcagta
      961 gccagagaac gcccggttcc gccaccaaca gtattcccca ggaaaccacc aagaacagca
     1021 ccgccaattg caccgataac gttggaatca tcaccgccct gaatctgtac cggacgtacg
     1081 ttaacgatgg tgccatagct gacattctgt acttgtttcg cttcagaagc ggtataaaca
     1141 tcccctgaca gggtgtcgtt attaacacaa ccgacaagag acagacctac cattgaaaca
     1201 accaatacgc gtttaatcat tgaaaaatct cctgttcacc atgaaacgcc acgcaagcat
     1261 ccctcatggt caaagtatat ggcatatgtg aatccatggt tacatgttct accaaatatg
     1321 agcaaatcat aatgaaagta ggatcaacca attataaaca aaacattcat gatgattatt
     1381 gatttgaaga taatgtttta ctaccaacgg taaaaatcgg tcattccgca tggtattcac
     1441 gaaaagttta tgtttgagtg gtgcgcaaac gtcagattga aaaaggatga actatgaaat
     1501 cgggccgctt tattggcgtt atgtcaggca ccagccttga tggtgttgat gttgtgttgg
     1561 cgacaattga tgaacaccgg gtcgcacagc tggcaagctt gagctggccg atcccggtgt
     1621 ctctgaaaca ggctgtactg gatatttgcc agggccagca gcttacactt tcgcagtttg
     1681 gacagcttga tactcaactc gggcgacttt ttgctgatgc ggtcaatgcc ttgcttaagg
     1741 aacaaaacct gcaggcgaga gatatagttg cgatcggctg tcacggtcaa accgtctggc
     1801 atgaaccgac gggcgtggca ccacacactt tgcagattgg cgataacaat caaattgtgg
     1861 cacgcaccgg aattacggtt gtcggtgatt ttcgccgtcg cgatattgcc ttgggaggac
     1921 aaggcgcacc gctggtacct gcgttccatc atgccttgct ggctcaccca accgagcgac
     1981 gaatggtgct caatattggc ggcatcgcca atctgtcact gctcattcct gggcagccgg
     2041 ttggcggcta cgataccggt cctggtaaca tgctgatgga tgcctggatc tggcgtcagg
     2101 caggtaaacc ttacgataaa gatgccgagt gggcacgggc gggtaaagtt attctcccac
     2161 tgctgcaaaa tatgctcagc gacccgtatt tctcgcaacc tgcaccgaaa agcaccggac
     2221 gcgaatactt taactacggc tggctggagc gccatttacg ccattttccg ggtgttgatc
     2281 cccgtgatgt tcaggccaca ctggcagaac tcaccgccgt gaccatttct gaacaagttt
     2341 tgttgagcgg tggctgcgaa cgattgatgg tatgtggtgg aggtagtcgt aatccgctac
     2401 tcatggcgcg tctggcggta ttactgctag gcacagaagt caccaccacc gatgccgttg
     2461 gcattagtgg cgatgacatg gaagcattgg ctttcgcctg gcttgcctgg cggacgctgg
     2521 cgggattacc aggaaatctg ccttccgtca ctggcgcaag ccaggagacg gtactggggg
     2581 ctattttccc cgcc
//
LOCUS       DAJTUN010000236         2524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTUN010000236 DAJTUN010000000
VERSION     DAJTUN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.242"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO470_003934"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO470_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6252997.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO470_003935"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO470_003935"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6252998.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..>2524
                     /gene="pgeF"
                     /locus_tag="OO470_003936"
     CDS             1884..>2524
                     /gene="pgeF"
                     /locus_tag="OO470_003936"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6252999.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGG"
BASE COUNT          581 a          639 c          684 g          620 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg caacgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtt cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcga
//
LOCUS       DAJTUN010000237         2457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTUN010000237 DAJTUN010000000
VERSION     DAJTUN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.243"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..187
                     /locus_tag="OO470_003937"
                     /pseudo
     CDS             1..187
                     /locus_tag="OO470_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            860..2368
                     /gene="ltrA"
                     /locus_tag="OO470_003938"
     CDS             860..2368
                     /gene="ltrA"
                     /locus_tag="OO470_003938"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6253000.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          616 a          606 c          734 g          501 t
ORIGIN      
        1 gagaggctac cagatggggc gctggcttgc tggcagactc atgaaagagc tggggctggt
       61 cagttgccag cagccgactc accggtataa gcgtggcggt catgagcacg ttgctatccc
      121 gaatcatctt gagcgacagt tcgccgtaac ggaaccaaat caggtgtggt gcggtgatgt
      181 gacctatagt gtgcccggag ttcagggcgg gcatggatgc ttaaatgaac cgcgagtctg
      241 tctggaatat tgaaccggta actcacgatg agaaacccaa caatcccacc gggtgtgacg
      301 gtggagaacc tgagcggcag tgacctgcgg catgcccgca gggtgatgta acccgctgac
      361 aacggggatt gaggcgagat cactaagccg agatgatcct caaggttaag tactgaaagg
      421 ctgaagaaca tgaacccgtt aatccgcctc tgtgggttga aaacgtcacc acggcctacg
      481 tgatctgaca ggccgtgcag gaggaactgg cagtgatacg taagcactgc cggtcgaagg
      541 tgttttgaca tgtatgcgaa acaccggggc agcagcgtct atcacgcttg cgttgctgac
      601 ttctgccaac ttgcggcaag caaggataaa gagtgcgacg ggcagcctcc tcagtatgcc
      661 tgagtccagg caggtaaacc ggggaaggtc agcgacggat gttaaggggg catggctccg
      721 atgacgcgct gactggcgga gcttccgtag tagtccgcga tggggaaagc ccattacatg
      781 gcgaagggaa gcagtttgaa tgtgtttgcg acgtgaatta actggcctaa cgaggtgaag
      841 acctttgata atcagcgaaa tgcaacgcaa gcttgccaca tgggcagcca ccgatccgtc
      901 cctacggatt caacggctgc tgcgtctgat aacacaacca gaatggctgg ctgaagcggc
      961 gcggatcacg ctttcatcaa agggggcgca tacccccggc gttgatggcg tgaacaaaac
     1021 aatgctacag gccagactgg ctgttgagct gcaaatcctc agggatgaat tactctcagg
     1081 ccactaccag cccttgcccg ccagacgggt ttacatccct aaaagcaacg gcaaactgcg
     1141 accactgggt atccccgcgt tgcgggatcg tattgttcag cgggccatgc tgatggcgat
     1201 ggagccgata tgggagagtg attttcatac gctctcatat ggcttccggc ctgagcgcag
     1261 tgtccaccac gcgatccgca cggtgaaatt acagctcaca gactgcggtg aaacccgggg
     1321 acgctgggtg attgaaggcg acctgtccag ttacttcgac accgtacatc atcgactgct
     1381 gatgaaggct gtacgccgca ggatcagtga cgcacgtttc atgactctgc tgtggaaaac
     1441 catcaaggcg ggacatatcg atgtcggtct ctttcgggcg gccagtgaag gtgtgccaca
     1501 gggcggtgtt atatcgccgc tattatcgaa catcatgctg aatgagttcg atcaatacct
     1561 gcatgagcgc tacctgagcg ggaaagccag aaaagatcgg tggtactgga ataacagtat
     1621 ccaacggggc cgaagtacgg cggtcagaga aaactggcag tggaaacccg cggtggcgta
     1681 ctgccgctat gccgatgatt ttgtcctcat cgtcaaaggc accaaagcac aggcggaagc
     1741 catcagggag gagtgtcggg gtgtgctcga aggcagtctg aaactcaggc tgaacatgga
     1801 taagactaaa atcacccatg ttaatgacgg ctttatcttt ctggggcaca ggatcattcg
     1861 caaacgcagt cgttatggcg agatgcgagt ggtctcaacg atcccgcagg agaaagccag
     1921 aaacttcgcc gcatcgctga cagcactgtt atcaggcaac tacagtgaaa gcaaagtcga
     1981 tatggctgaa caactcaacc gaaaactgaa aggctgggcc atgttctatc agttcgttga
     2041 ttttaaggcc aaagtcttca gttatatcga ccgtgtcgtg ttctggaagc tggctcactg
     2101 gctggcccgc aaataccgta caggtatcgc ttccctgatg aggtggtggt gtaaatcacc
     2161 ggaaccgggt cagagcaaaa cgtgggtttt atttggtaaa accaatcacg gcaagctcag
     2221 cggcgaaata ctgtaccggt tggtggggca aggcaagaag ctgttccgct ggcggctacc
     2281 cgaaggtaat ccctatctga ggacggagac cagaaacacg tatacatcgc gctttacaga
     2341 agtggcaatg gcgttcgcca gcatttaaat ggagagccgg atgcgctgaa aggtgcacgt
     2401 ccggttcggg gaggagaggc agggaaatag tccgactacg ccctgcctct tactcta
//
LOCUS       DAJTUN010000238         2442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTUN010000238 DAJTUN010000000
VERSION     DAJTUN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.244"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..568
                     /locus_tag="OO470_003939"
     CDS             <1..568
                     /locus_tag="OO470_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6253001.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            526..1203
                     /gene="bglJ"
                     /locus_tag="OO470_003940"
     CDS             526..1203
                     /gene="bglJ"
                     /locus_tag="OO470_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6253002.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="OO470_003941"
     CDS             complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="OO470_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6253003.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2170..2406
                     /locus_tag="OO470_003942"
     CDS             2170..2406
                     /locus_tag="OO470_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6253004.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          536 c          627 g          651 t
ORIGIN      
        1 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
       61 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      121 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      181 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      241 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      301 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      361 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      421 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      481 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      541 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag
      601 agtttatcca gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag
      661 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
      721 atgagaagtg agcgcagaga gggattatct tgcctgactg agctggcgat taagtttccg
      781 cgtacccggc gtttagttat tgtggatgat gatattgaag ctcggctgat tggttcattg
      841 tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag
      901 gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg
      961 tacattaacc aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg
     1021 cgtggctact caatgacaca aattgccgag cagcttaaac gtaatatcaa aacgatccga
     1081 gcgcataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag
     1141 gccgcagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat
     1201 taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatagc
     1261 cacattgctg cacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc
     1321 cgtcgcgcag taccacggta cgccagagtg gattatcttc accgttcgtg agcgttttct
     1381 caaaaaagag ggcatggcgc agcgattcaa cagtagcctc gccgagcagt tgcttcatct
     1441 cagtgagata ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct
     1501 ctccggtcgc ttctagtgct tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca
     1561 ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac
     1621 aggcgacgcg tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg
     1681 ctttttcctg cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt
     1741 gtgcccatag tgagatcagc ggtttgttct cgcggatcat cgttggttgg ttgcgataga
     1801 tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca
     1861 gcgtcatggc gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac
     1921 gatgtttggc gatcatcgcc cgtacggctt gtgcaagccc agcatcctgc ggctggagat
     1981 gcgttcgcca gatgacatct tcatagagcg gtgcggaacg ataggccata atcgtgatag
     2041 taatctaaat gataatgatt gctaatccta gcgataggtt tactcgatag caagggattt
     2101 ctctggcttg caaatgataa aaattatcat atgatattgg ttttcattat caatgaaaga
     2161 gatgaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg
     2221 ctgattgcag ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg
     2281 atgggattag tattgacgcc ggtgatgctg tatcacaaac agttgcggca ttacattttg
     2341 ctaccatcgt gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag
     2401 ggatgacaaa acagggaaga aacaagaaag gaagtaaaga ta
//
LOCUS       DAJTUN010000239         2431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTUN010000239 DAJTUN010000000
VERSION     DAJTUN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.245"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(1..1270)
                     /gene="flhA"
                     /locus_tag="OO470_003943"
                     /pseudo
     CDS             complement(1..1270)
                     /gene="flhA"
                     /locus_tag="OO470_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
     gene            complement(1263..2410)
                     /gene="flhB"
                     /locus_tag="OO470_003944"
                     /pseudo
     CDS             complement(1263..2410)
                     /gene="flhB"
                     /locus_tag="OO470_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          602 a          727 c          593 g          509 t
ORIGIN      
        1 cctttcatca aaatgcgata gcgggcaggt tgcagatcca tattgtcgcg aatgtgcacc
       61 actggcggca gaaatcccat ctcctgggca aatttcttgc ggatactgcg tatacggccc
      121 aacaactcac catcctgctg gaaatcgacc atcgggatca gtcgataacc cacttccatt
      181 cccagagaat cttccagttg tacatcgttc cacgtcgctt cgacaacggt attattctct
      241 gccattttta ccggtttggg ttcggcaggc gctttttgtt cgcgtccgcg tatccaccag
      301 gccagcccca gcaatccggc agtgaacagc aaaaatacca ggttcggcat tccaggcacc
      361 aggccgagta aaccgagcac ggcggcgctt aacaacataa cgcttgggtt actgaaaagc
      421 tgattcacca tctgctcgcc aacatcctga tcggtgctga cacgcgtaac gatgcccccc
      481 gcggcggtag aaatcaccag cgccggaatt tgtgccacca gaccgtcgcc aatggtcaat
      541 agcgtataac tttccgccgc gtgtcccatg ctcatgccag ttgcagcacg ccgaccagca
      601 acccgccgac aacgttaatg accatgatga ggatcccggc aatggcatcg ccgcgaacaa
      661 acttacttgc cccgtccatt gagccgtaaa aatcggcttc ctgagtcact tcggagcggc
      721 gttttttcgc ctcatcttca ccaatcaatc cagcgttaag gtcggcgtca atcgccatct
      781 gcttacccgg cataccatcg agaacaaagc gcgcacccac ttctgctata cgcccggcac
      841 ctttggtaat gaccataaag ttgatgatca cgagaatgac aaacaccacg ataccgatag
      901 cgaaattgcc accaacgagg aagtgaccga acgcttcgac caccttccca gccgccgccg
      961 cgccggtatg cccttccatc aaaatgatac gggttgaagc cacgttaagt gccagacgca
     1021 acaacgtagt aaacaacaga atggtcggaa acgcagcaaa ctcaagcgtg cgctgggtaa
     1081 acatcgccac cagcaacacc atgatcgaca aggcaatatt gaaggtaaac aacaggtcga
     1141 gtatgaatgc gggcagtggc agcaccatca tcgacaagat caacaggatc aaaatcggtc
     1201 cggcaaggat ctgccattat gtcgatttca ggtttgcggg caggcgcagc atcgcggcca
     1261 gattactcat gggtcggttt ctcgttaata aaatccaggg ctgccggcac cggaagatga
     1321 gtaggtgtac agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagacccagg
     1381 ccagcacttc cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccgcat
     1441 gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc
     1501 caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc
     1561 tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga
     1621 catccgcttt cggcacatcg gccatcatcc gacgccgtgc ggcagctcgc tgcatctgac
     1681 agatccgtcc tttaacatgg gggtcgcctt cgccttgttt gaactcatca cgaatatcct
     1741 gccgcgacat tcgcagcttt ttcaggtggc tgaagatttg gaaaaagacg tcaaatccca
     1801 ccattggaat gacaccaagc accaccagca gtgcgcatag ccctaccaaa tccatcgcat
     1861 tacccatggc ggtaatcggt gactcggcca ttaagcgcat catctgcggc cagtgatgcc
     1921 agagaaaaaa ccccgtcacg ctgccaacca ggatggtttt caaaattgcc ttaagcaact
     1981 ccgcgctagt ctgtgccgag aatatccgtt taatgcccgg tagcgggttg agtttggaaa
     2041 acttcggctg caaggatttg ccgctaaata ccagcccccc cagcataacc ggagaaatga
     2101 gcgccaccag caccacgccg ctaatcagcg gtagcagcgc cagcatggct tctctgatca
     2161 gcagaataat ttgcccgagg atcagattcg ggtctttgat aatactgtga tcaaaatgca
     2221 gcccagcgga gagcatgccc gacaatcgac gggccagcga cacaccgcca aaccagataa
     2281 cactaacgcc caccagcaaa atcagcagtg aggtcagttc acgggaacgc gggatttgcc
     2341 cctcttcccg cgctttttct agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct
     2401 cgtcagacac gtcgccaatc ctggaaagag t
//
LOCUS       DAJTUN010000240         2425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTUN010000240 DAJTUN010000000
VERSION     DAJTUN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.246"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(64..822)
                     /locus_tag="OO470_003945"
     CDS             complement(64..822)
                     /locus_tag="OO470_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6253005.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO470_003946"
     CDS             complement(830..1933)
                     /locus_tag="OO470_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6253006.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2425)
                     /locus_tag="OO470_003947"
     CDS             complement(1943..>2425)
                     /locus_tag="OO470_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6253007.1"
                     /translation="LFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIA
                     EIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSL
                     ATAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER
                     "
BASE COUNT          663 a          647 c          566 g          549 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggtggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaag
//
LOCUS       DAJTUN010000241         2322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTUN010000241 DAJTUN010000000
VERSION     DAJTUN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.247"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(63..431)
                     /gene="folB"
                     /locus_tag="OO470_003948"
     CDS             complement(63..431)
                     /gene="folB"
                     /locus_tag="OO470_003948"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6253008.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            536..1153
                     /gene="plsY"
                     /locus_tag="OO470_003949"
     CDS             536..1153
                     /gene="plsY"
                     /locus_tag="OO470_003949"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6253009.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(1166..2097)
                     /gene="ttdR"
                     /locus_tag="OO470_003950"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(1166..2097)
                     /gene="ttdR"
                     /locus_tag="OO470_003950"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT          553 a          620 c          523 g          626 t
ORIGIN      
        1 ccgcttgtcc gaactactgc agtatgacca ggttataacc gtttggttta acagctgtaa
       61 aattaattat tttctttcag attattgcca cgctcaatga ttacgccaac attcgccgcc
      121 cgcgccactg cgcctggctt gctgagtttg atacgcaccc acggcgagtt gaagcgtgct
      181 aacagcagct ccgccacctc ttcagccacg cgttccacca gcgcaaaacg cgccccctcg
      241 acgtggctga ccaccgtttc tgcaatgtca gcgtaactga ggcaatccgc cacatcatca
      301 cttttcgccg ctttacggtt atcccatgcc atttcgatat cgaacactaa cttctgttcg
      361 atggtctgtt cccagtcgta aacaccaata gtggtgatta ccgaaagttg ctctataaat
      421 acaatatcca tcacgtcctg cctgcttttt ggctaaccgg ataccacttc cggcgaaatg
      481 tgcgtattat ccacagattc atcgttgaac acgaattttc aaaacggaac agcttatgag
      541 tgcaatcgcg cctggaatga tcctcatcgc gtacctctgc ggctccattt ccagtgccat
      601 tctggtttgc cgcttgtgtg ggctgcccga tccgcgaacc agcggctccg gcaatccagg
      661 cgcaaccaat gtgttacgta tcggtggcaa gggagcagcc gtagcagtac tgattttcga
      721 cgttctgaaa ggaatgttgc ctgtctgggg cgcgtatgaa ttaggtgtca gccccttctg
      781 gctaggctta attgccatcg ccgcctgtct tggacacatc tggcccgttt tcttcggttt
      841 taaaggagga aaaggcgttg ctaccgcttt tggagcaatc gcaccaatta gctgggatct
      901 caccggagta atggcgggaa cctggttact gaccgtgcta ttgagcggat actcgtcgct
      961 gggagcgatt gtcagtgcac tgattgctcc gttttatgtc tggtggttta agccacaatt
     1021 caccttcccg gtttcgatgc tctcttgcct gatcctgctg cgtcatcatg acaacatcca
     1081 gcgtctgtgg cgtcgtcagg agacaaaaat ctggacgaaa ttcaaaagaa agcgcgaaaa
     1141 ggatcccgag tgatttctgg tggatctaca tgacctgata gccttcatcg ggcttgccca
     1201 gccgttgctg gcaccatgcc gccagaaatt ccgcgcagac acgaatttca tgctgcgata
     1261 gagcagctcc cggtaaacag cccagatatt ggcgctttgt gcatactccg gcaatacttg
     1321 caccagtttg ccactctcca gaaacggcaa cacatcccac tcggaacgca gcataatccc
     1381 tttgccctcc agcgcccatt gcaacactat ctcgccgcta ttggaggaaa gatgcccgct
     1441 aaccttcacc gattttttct cctgaccgtt ccccaactcc catattccgt gggtcatatc
     1501 gcgctctttg gtcaccagac agtcatgacg acttaattcc tgtaatgatt gtggctgcgg
     1561 atatttttgc agatactcag gtgctgcgca taatattctt ttatttttcg ttaacagatg
     1621 cgcaatataa taatcaggaa tttcgtcatt aatacgaata tccagatcaa tattatcctg
     1681 cactaaatca atttgccgat cgaacaattc aaaatgcacc tgtaactcag gataattgcg
     1741 cataagttcg gtaatggctg gcgcaatatg gctgcgccca aaaccaaaac tgcaaccaat
     1801 acgaatcatc ccctccgggc gcgttttgat ttgcgtgacg tcatcgacca gtcgctgata
     1861 ttgggtgagg atttccagcg catgttcgta acaacgttgg ccactttcag tcagcgccac
     1921 gccgcgagcg gagcggttca gcagcgttgt tgccagcgta ttttcaagga tttggatccg
     1981 cttggtgaca aatgcaggcg tttgcccaag cgtcgccgct gcagcactaa aactcccgct
     2041 gtggacgatt tctaccagta cctgcaagtc tttggctaaa ggccagctat tcagcatcgt
     2101 taattatccg cagttgtgat aagcgcagtg tatttcgcaa aacattgcca ttccctgctc
     2161 tggcgcaata ttcattctta ttttcgcgtt gattcacgaa ctgttaatta catattcacg
     2221 gtggcaaaaa atataaaacc acatttttag tgttagtttg tggcggtgaa tttttccaga
     2281 caaatacaaa aactggagtt gccatgatga gcgaaagtaa ta
//
LOCUS       DAJTUN010000242         2123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTUN010000242 DAJTUN010000000
VERSION     DAJTUN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.248"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="OO470_003951"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="OO470_003951"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6253010.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..>2123
                     /locus_tag="OO470_003952"
     CDS             1649..>2123
                     /locus_tag="OO470_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6253011.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLV"
BASE COUNT          579 a          445 c          546 g          553 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc
     1981 tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
     2041 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc
     2101 aacggataaa ccaaacctag tgt
//
LOCUS       DAJTUN010000243         2081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTUN010000243 DAJTUN010000000
VERSION     DAJTUN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.249"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(57..326)
                     /locus_tag="OO470_003953"
     CDS             complement(57..326)
                     /locus_tag="OO470_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6253012.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            383..1051
                     /locus_tag="OO470_003954"
     CDS             383..1051
                     /locus_tag="OO470_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6253013.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1367..>2081
                     /locus_tag="OO470_003955"
     CDS             1367..>2081
                     /locus_tag="OO470_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6253014.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGP"
BASE COUNT          627 a          465 c          386 g          603 t
ORIGIN      
        1 accagccaac cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac
       61 attgtgtctg gttcttccgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc
      121 agcaacaccc gatacttctt ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc
      181 tccagatcta cagcatcctg aagtggcgca atatgctcac tgaatccctg gatgtagaac
      241 tgtgtggtga cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt
      301 tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt
      361 aattcacaaa taaaactata atatggtgaa attaatgaaa aaaaacacag atgatggggc
      421 taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa
      481 tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt
      541 aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc acgtacctga
      601 gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag
      661 ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac cttatcctgc
      721 ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg
      781 aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgatgatgg
      841 aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa
      901 actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg
      961 cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt
     1021 agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag
     1081 acgtgctacg gtttccttta cgatttgggg ttgcgacttt acctctattg ataatgcatt
     1141 ccggcagaac gttcaaatat gaacgtacga tatttaacta accgaaaaac aaaatatagc
     1201 acaggcgaga catttaccat ctaagaaaat agtatcgttt ttttacagtt aaatcaacat
     1261 cacttcctta aaatgaaaac aataaatttc aatcctgaaa cattttattt cagcttatta
     1321 ttccccagta tcacatttta tcaggatatc cttctgcgag gttattatgc ttcctgtaaa
     1381 taatcccccc ctatccactg gaaacgtctc tttttacaga actacatcaa tcgacaatgt
     1441 tcacaataat tatctctccg aatgggttga atggactaaa aacagcattt ccggagaaaa
     1501 cagggaaact gcttttaccc ggctccaatt atgtctggag aacagtgaaa catcgttgga
     1561 cttatcttgt ttaggtctca gatctctacc acgattgcct gacaatcttg atgaaattaa
     1621 tgtaagcaat aaccaactat caatgctccc cgagctacca agggcattga aagagctgaa
     1681 tgcaagcagt aatcaattat ctgcacttcc tgaattacca gtgtcgctgg aatatataaa
     1741 tgtgagtgat aaccatttgt tcgcacttcc tgaattacct gcgtcactag aatatattaa
     1801 tgtaagtgac aatcacctgt ctgtacttcc gaggttacca atgtcattgg aattacttga
     1861 tgcagccaga aatgctttgg aagtaatacc agattttcca gaaagagatg atcatattat
     1921 aagaatattc tggcttaatc agaaccggat cacggcaatt ccggaaagca tacttggcct
     1981 cagttctgat agcgttgtca atcttagaga aaatcaacta tctcccagaa taatgcaaac
     2041 tttgttacaa caaaccgccc aaccggacta ccacggccca c
//
LOCUS       DAJTUN010000244         2067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTUN010000244 DAJTUN010000000
VERSION     DAJTUN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.250"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            185..589
                     /locus_tag="OO470_003956"
     CDS             185..589
                     /locus_tag="OO470_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6253015.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            615..1328
                     /gene="msyB"
                     /locus_tag="OO470_003957"
     CDS             615..1328
                     /gene="msyB"
                     /locus_tag="OO470_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6253016.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            1477..2043
                     /gene="satP"
                     /locus_tag="OO470_003958"
     CDS             1477..2043
                     /gene="satP"
                     /locus_tag="OO470_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6253017.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
BASE COUNT          528 a          506 c          508 g          525 t
ORIGIN      
        1 gcgatatggc aggacaagac gtaaaattga cagcaatttt attcatataa agaatgaatc
       61 gcagccaagg cgttcatcaa aaaattgtaa taaaaagaaa agattacgtg cctgaatctt
      121 ctctttatca gcagtaaact agtgggtatt catcccccta cctcttccca ctaagagaat
      181 ccttatgaaa tccgttttta cgatttccgc cagcctggcg attagcctga tgctgtgctg
      241 cacggcgcag gcaaacgacc ataaactcct cggcgtcatt gcaatgccgc gtaacgaaac
      301 caacgatctg gcgctgaaac ttcctgtttg tcgcattgtg aaacgcatac aactctctgc
      361 cgaccatggc gatttacagt taagtggtgc atcggtttat ttcaaagccg cccgtagcgc
      421 cagtcagagc ctgaatattc cttcagaaat aaaagaaggg caaaccactg actggatcaa
      481 cattaacagc gataacgaca ataaacgctg cgtctcaaaa atcacctttt ccggtcatac
      541 ggtgaactca tcggatatgg ccacgctgaa aattatcggc gacgactaac gccagaatat
      601 agaagccaca aaaaatgaat gttaattacc tgaatgattc agatctggat tttcttcagc
      661 attgtagtga ggaacagttg gcaaatttcg cccgattgct cacccataat gaaaaaggca
      721 aaactcgcct ctccagcata ctgatgcgca acgaactgtt taaatcgatg gaagggcatc
      781 ccgagcaaca tcgccgcaac tggcagctga ttgccggaga attacagcat tttggtggcg
      841 atagtatcgc caacaaactg cgcggacacg gtaaattgta tcgggccatt ttgctcgatg
      901 tttcaaagcg attgaagctg aaagccgaca aagagatgtc tacgtttgaa attgagcaac
      961 agttactgga acaatttctg cgtaatacct ggaagaaaat ggacgaggaa cataagcagg
     1021 agtttctgca cgcggtcgat gccagggtga atgagctgga agagctgctg ccgctgctga
     1081 tgaaagacaa attattggca aaaggtgtgt cgcatttgct ttccagccaa ctgacccgca
     1141 ttttacgcac ccacgcagca atgagcgtac ttgggcatgg tttgctgcgc ggcgcggggc
     1201 tgggaggccc tgtgggcgca gcattaaatg gggttaaagc ggtcagcggc agcacctatc
     1261 gcgtgacgat tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt agcgccactc
     1321 aggtctgata ccggaaaccg gggaaaattt tcattttccc ctcaaaagat cgtagacact
     1381 gccccactgg ctgattatta tgccgcgccc tgaaaacact acagttattc agggaaatta
     1441 tttcaccatt cattcgatga tgatttttga ggaattatgg gcaacactaa gttggctaat
     1501 ccggcaccgc tgggcctgat gggcttcggc atgaccacca ttctgcttaa cctgcacaac
     1561 gtgggttatt tcgctctgga cggtattatt cttgccatgg gcattttcta cggcggcatc
     1621 gcgcaaattt ttgctggtct gctggagtac aaaaaaggca acactttcgg tttaaccgca
     1681 ttcacctctt acggttcttt ctggctgacg ctggttgcga ttctgctgat gccgaaactg
     1741 ggtctgaccg atgcgccaaa tgcacagttc cttggtgtct acctgggtct gtggggcgta
     1801 tttacgctgt ttatgttctt cggcactctg aaaggcgcgc gcgtcctgca attcgttttc
     1861 tttagcctga ccgtgctgtt tgccctgctg gcgatcggta acattgccgg taatgccgcg
     1921 atcatccact ttgccggctg gattggtctg atctgcggtg ccagcgcaat ctatctggcg
     1981 atgggtgaag tgctgaacga gcagtttggt cgcaccgttc tgccgattgg tgaatcccac
     2041 taattgtctt gagatccccc cgctcgg
//
LOCUS       DAJTUN010000245         2025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTUN010000245 DAJTUN010000000
VERSION     DAJTUN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.251"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..236)
                     /locus_tag="OO470_003959"
     CDS             complement(<1..236)
                     /locus_tag="OO470_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6253018.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITIL"
     gene            complement(240..983)
                     /locus_tag="OO470_003960"
     CDS             complement(240..983)
                     /locus_tag="OO470_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6253019.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(874..>2025)
                     /locus_tag="OO470_003961"
     CDS             complement(874..>2025)
                     /locus_tag="OO470_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6253020.1"
                     /translation="DVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLD
                     RWMVDPALTRRIKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKM
                     TENEWGMSVVERIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEAL
                     LKNIDLIRQFQSNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLF
                     GQSPKGFSTGDADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFN
                     PLWQMSDVDRSTVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIE
                     NAEDEAPPGIGEPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
BASE COUNT          435 a          588 c          516 g          486 t
ORIGIN      
        1 tgcaggatcg tgatgctcat cccttcgaat gaggcaagcg tggccggatg gaatacctgc
       61 tcaggagaac gcgtgacgac tatttcaacg aacttatcgg gtttcagttt tggcaggtca
      121 tcagcaccat agagctgctt acctgttcgt cctatcggca cgtctttgca cagcaacgag
      181 ccgtcagcca ggcggtatcg ggtttccccg agcctggtct caaaaaagta tttcatgggt
      241 tatgctgccc ttttcattgc gaatggaacg cgaacaaaca cgatctcttt atagcagcgg
      301 cagtttggca gctcgcccgc gtggccggtc atgccgtcca aagttggagg tgaatcccag
      361 cggacgaatt tacctttcat gtgatcgtgg gaatctcgga catcgccatc atcggctgta
      421 cgccaaatat aaccatccga gccaatagct atcgctcggg ccatatccag cgcgcccgtt
      481 gctcttccca gttccgttcg ggcaatcaga tcggccctag acttagcaac atcgccggta
      541 gatgcaatct ctttagcaaa ctgctccgcc cgaccaccgg tgacaacagc ctctatcgct
      601 ttgttctgga tgtcgtacac cctgtctgcg gcctcgagag gcagagattt gatgtacttg
      661 acctgttcgg cgatgatgga ttgcatcacc tggcccacag gagcgctttc cacaagattg
      721 cggagctcgc gactgatgtt cttgctgtgt tgccgccaaa ctttctcgtt ctgccgggtt
      781 aggtccgcag taaagttttc cgcgaccttt gtcgcccagg gggtgatgat ttcactgtaa
      841 cgctccagcg ccgcaataat ttccgtgata ctgtcatttg aaccatcgta gcgaccattt
      901 acgatgtctc cgaccgccct cgctatcctg cgtaggctgg ttcgataccg gatttccgcc
      961 tgacggctcc tgcggttcgt catcaggttc gccgatgccg ggcggcgctt catcttcggc
     1021 attctcgatg tcctcgttgg taatggatgc ccctatgccg gttacgtcag aattttctcg
     1081 caaatcagtc atagcggctt tcagtgtcat cagaccatca cccagcgccg tactgattgc
     1141 gttggtggtg tttaacgcca ccgttgagcg atcgacatca gacatttgcc agagcgggtt
     1201 aaactcaaac gtgaaatcgt ccggcagcgg cttgccaagt tccgaacgat gcatgatgtc
     1261 cagtatccgc cgcaccggaa gacgtaaacg cctctcctgc aacgaactga tgcgatcgta
     1321 atagttggca aggtctgcat caccggtaga aaatcctttc ggggactgtc cgaacaaccg
     1381 caccagtggg ataccaacag cgccactaat ctgttctgca aactgcgaaa ggatgtcatc
     1441 cagaccactg aagctgtact gatgggtttc aaacttatcc cgcgagtcca tgagcgtcat
     1501 gccttcatta ctctggaact gtcgaatcag gtcgatattc ttcagcaacg cttcatacgc
     1561 aggaccacca agtgcgataa gctcgcgtag cttctccacg ctgtaggtac gcaaatgcgc
     1621 cttgtagacc agctgcgccg cgccgacagt agcgctgtcg aacgcggtaa gacgatccca
     1681 gatacgctct acaaccgaca ttccccattc gttctcggtc atcttctgct gaaatggcaa
     1741 cgtgacgcca tcaaagcgaa tcaggcgact gtgatgaatg cgccaggcag gaattcccgt
     1801 tgctgtggtc accacatcgt aaaactcagg tttacccagg tccggcccca tatctttaat
     1861 gcggcgggtc agtgccgggt cgaccatcca gcggtcgagc gggagaatcc ccttaaactt
     1921 gcccttaccg atggtttcgg gtcgcagcgg ggtcattggt gcctgcccct caatcatgat
     1981 gaaacccacc gcgccgccgt agaggcgcga ccatttcagc acgtc
//
LOCUS       DAJTUN010000246         2015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTUN010000246 DAJTUN010000000
VERSION     DAJTUN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.252"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            6..224
                     /locus_tag="OO470_003962"
     CDS             6..224
                     /locus_tag="OO470_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6253021.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO470_003963"
     CDS             226..591
                     /locus_tag="OO470_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6253022.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="OO470_003964"
     CDS             588..1253
                     /locus_tag="OO470_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253023.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO470_003965"
     CDS             1253..1618
                     /locus_tag="OO470_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6253024.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          551 a          417 c          537 g          510 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatct
//
LOCUS       DAJTUN010000247         1947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTUN010000247 DAJTUN010000000
VERSION     DAJTUN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.253"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            236..1849
                     /gene="mppA"
                     /locus_tag="OO470_003966"
     CDS             236..1849
                     /gene="mppA"
                     /locus_tag="OO470_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6253025.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          543 a          428 c          477 g          499 t
ORIGIN      
        1 ctgattatga taccgaaaac tggtttgaac gtgcgaagcc cgaacgtagt gttcggtgtt
       61 ctatgtgctt tgccacattt tggcgtctat tatttacact aaatctgatt tgatatattg
      121 atacttaaaa catttgatgc ttcctttgtc acttttttga tggaagttgt ttgcatttct
      181 ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga aacttatgaa
      241 gcactctgtt tcagttacgt gttgtgcgct gttggtcagc agcatttctc tttcgtatgc
      301 tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc gccacattaa
      361 agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga ttcaggtcat
      421 tcgcgatctg tttgaaggtc tggtgaatca gaacgaaaaa ggggagattg tccccggcgt
      481 cgcgactcaa tggaaaagta atgacaaccg tatctggact tttaccctgc gcgataacgc
      541 aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct ggcaacgtct
      601 ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg gaatcaacaa
      661 cgcacaggcg attattgatg gtaaagctac gcctgatcag cttggcgtca ccgcagttga
      721 tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga atttaaccgc
      781 taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag agtggacgaa
      841 acccggaaat ctgatcggca atggcgctta tgttcttaaa gagcgcgtag tcaatgaaaa
      901 actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc tgcaaaaagt
      961 gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacctcg cgggggatat
     1021 tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg atattccggg
     1081 gcaggtttat acgccaccgc agctcgggac ctattattat gcctttaaca cgcaaaaagg
     1141 gccgacggca gatcagcgcg ttcgcctggc attaagtatg acgatagatc gccgcctgat
     1201 gaccgaaaaa gtgttgggga cgggcgaaaa gccagcgtgg cattttatac cagatgttac
     1261 cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag aactgaatgc
     1321 gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc tgaagctgac
     1381 gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag catcgatgtg
     1441 gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa cctatatcga
     1501 tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtggggg attataatga
     1561 accctccact ttcctgacat tattaacgtc aatgcattca ggaaatattt cacgctttaa
     1621 caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg ttaaagcgcg
     1681 taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga ttgcaccaat
     1741 ttatcaatat accaatggac gattaatcaa accgtggctg aaaggttatc ccattaataa
     1801 ccctgaagat gtagcgtaca gtcggactat gtatattgtg aagcattgat gtgatgggaa
     1861 ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgcccct gttccggcgg
     1921 tgtaatgtta tccatataca gcgtctg
//
LOCUS       DAJTUN010000248         1871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTUN010000248 DAJTUN010000000
VERSION     DAJTUN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.254"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..364)
                     /locus_tag="OO470_003967"
     CDS             complement(<1..364)
                     /locus_tag="OO470_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6253026.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(431..1645)
                     /locus_tag="OO470_003968"
     CDS             complement(431..1645)
                     /locus_tag="OO470_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6253027.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          567 a          484 c          379 g          441 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aataattaag
      421 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatact
      481 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      541 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtacgggaaa acttcaagca
      601 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      661 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      721 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      781 tgatcgtcgt gcgaatatag ccaatcagct gctcttcgat taactgcttg cagtcccaga
      841 tactgttaaa cacttcaccg accgcaatcg gcgtcacggt atgctggcga atcagacgaa
      901 aacattcctg gttctcggca ggcgtcggat cttccatcca gaacaggcgg taatcctcaa
      961 tgctcttccc aaagcgcgcc gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca
     1021 gatgttcgtt aaaaccgaac ttattacgca ccgcttcgaa taacttcggc gtaaaatcga
     1081 gatacttttc tgaagaccac aactgctctt ctggccacag gcctttggtt gccggttcat
     1141 acgccagtcc tttgcccttc gccaggccat aagtggtttg catacccggt acaccgcact
     1201 gcacacgaat cgccttaaag cccatctgct ggtgctttgc atagtcttca aggacttcat
     1261 caatcgtacg cccggtggtg tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa
     1321 gcagttgata caatggcata tttgccgctt tagctttgat atcccacaac gccatatcaa
     1381 tggcagaaat ggctgacatc gtgacggggc cacgtcgcca gtaagcacct ttataaaaga
     1441 attgccagat atcttcgata cggctggcat cgcggccaat tagttgtgga caaagatgat
     1501 ctttcagata agaagcgacc gacaattcgc gaccatttaa cgtggcatct cctaatccac
     1561 aaagtccgct ttcagtggtt attttaagcg taacgaaatt acgccctggg caagtaacaa
     1621 aaacatctgc agcaataatt ttcattattg caatctcctg tgttgtaaat agacgtttca
     1681 aacctaattt tacgcctact accatacaag agagaaaaag cgattattca accgtgatca
     1741 cattaaaaac aaaaatttcc accgaggaga aaaaataccc tcaaaaacca cctgaataat
     1801 taaccattat tgttaaaaca acacgttaaa taatcacata acaaactcca ttaatcattg
     1861 aaaatgagca c
//
LOCUS       DAJTUN010000249         1837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTUN010000249 DAJTUN010000000
VERSION     DAJTUN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.255"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(494..1666)
                     /locus_tag="OO470_003969"
     CDS             complement(494..1666)
                     /locus_tag="OO470_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6253028.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
BASE COUNT          573 a          328 c          356 g          580 t
ORIGIN      
        1 tactcaacgg cacaacgttc cagagcgcag gggattatca gccaagtgca gaacgaccat
       61 actggataac aggagctaat tgcgaggtag ttgacattgg gtgtgttttt acagaaacaa
      121 ccagtaaggt aacatcggct tctgcattgt cagtcacacg ctcgggaaaa attaacgcca
      181 atggtagtat cgacgttgct actggtgttt catcggttaa cgtggtttct acaggtgtgt
      241 atgatgttag cttttctcat ccgctcgcag cagttgctag cggttatatt gtgcagataa
      301 cacctatatc tgcgcctgat tcagttagtt gtgatgttac ttatattggc gtagatacat
      361 ttagggtgac cctgcgcaat acgcttagcg gggcaggaat ctccagttcg tttgcattta
      421 gcattaccag actaatataa atacattcgc cgccccattt ggacatatat gtagggcggc
      481 ttatgtttat tgtttattga ttgttgtttg ttaatgtggg tattaattct tttactaaag
      541 ttgtggtttg ttttgttaat ttgatgaatg gcgtctctat taatttaaaa gttaggcatg
      601 atgtgaacgt aataagaaat gcagttgtgc ttaccagtat gataaatgta ttttcagtgt
      661 aattattagg aataatccac gtcattaggc aataaaggaa tatcccatgc agaaggtaaa
      721 cgctgtaggt agtttctcct agctttctgg ttatatttaa tcttaatatt ccatacaaat
      781 cacaacctga tgcgataatg ataaatgtta ttccacaaag aatcagtggt agcggtgcgt
      841 aagttgtttt gaaatacgtc atttcaaaaa ccattatcgc agttattata attggtgtta
      901 cttttgcttt ggcaattcca ttgactatct ttgatttatt tagcaaaaat gccaggagcc
      961 caaaaagaaa tgatgctatg tgtattttac tgaaaaataa aatgaaaaca aatagcgata
     1021 ttgcgctaat cataaccatg taaatactga cttttctttt tatgagcgcg gaaattaccg
     1081 gaagagaaaa ataaaagaac cattcatata caagtgtcca tgttacagcg gcattgatag
     1141 taaatgaatc ttttacgtca ttaatgttcg gcatacctag cgcagtgaat ggcaaccact
     1201 tcattattga cacaaaaagc tcttctgtgg atacctgcat tctccatcca gacttaaaac
     1261 ctacaatgat aaaaatgaga cataggctaa ctatgaacat tggggttaat cgtagtaatc
     1321 ttgatacata aagccttgtc cagtcctgat ctccagagat aatctttgaa aagaacagat
     1381 aaccagtaat cataaaaaag aatgacacac caacttggcc taaatttgct aacagatttg
     1441 atgaaggtgc ttcccatact ccagatgaca agtatccatt ccaaattgct gcgtgatgaa
     1501 taagcacgaa aattgctaag aacccccgca ttccatctat ttgattattc ctgccgtgct
     1561 caacaagatt tattggggaa attttatcaa ataaccccac agctaataac gacatcaata
     1621 tcacggatgt tattaataat aaactgttaa tttcaatcat atgcatgctc tattacgcaa
     1681 ttaaaatttc tccaagtata ctgactttta gttaatgatc atatagttat gtgtaactga
     1741 tgaaacaaaa cggagacaca ccaagctttg cactggattg caaggctttg tgctcttctg
     1801 tgaatgtgtg gctatatgtt tgaagatcgt tgtgccg
//
LOCUS       DAJTUN010000250         1824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTUN010000250 DAJTUN010000000
VERSION     DAJTUN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.256"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..536)
                     /locus_tag="OO470_003970"
     CDS             complement(<1..536)
                     /locus_tag="OO470_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253029.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRL"
     gene            complement(1191..>1824)
                     /locus_tag="OO470_003971"
     CDS             complement(1191..>1824)
                     /locus_tag="OO470_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253030.1"
                     /translation="GILCSAATHVSDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVV
                     DVLKDTSDLQIIEAAPLSGKPSDQAEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAI
                     LDRAAQRAGHEGISELLGCTVCGYAGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTR
                     NSNTAGYDLELISVVDVLATGSVKVKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          521 a          414 c          389 g          500 t
ORIGIN      
        1 agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg cgtagacata
       61 taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg gtctgatact
      121 aatgccatat agccccataa actatccctc agccagacag catcatagtg ggtttctgcc
      181 gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg tttctccgtc
      241 aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc gtctttggta
      301 tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg ataattagga
      361 tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct ggaattaaga
      421 atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt gccgcacgaa
      481 aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata atccatcaat
      541 cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg tattttacct
      601 gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa aatggggaag
      661 tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag aaaatcagat
      721 acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa gggaacctag
      781 gtatcgaggg tatcgaagat aatcagccgc taaccatttg tttccttaaa ggatgatgaa
      841 tccttgtggt tacagttatg gttacacatt aatttatctc tggtcaagtc ccgaagtcct
      901 aactttaaga cagtggcccc aatcgtccag acttgaaagg ggcctatggg ggaagtcgct
      961 gacctttatc tattaaatac ctacagagat ttttgtgaga ttttttaatc caaggtcata
     1021 acctactgcc cccacaggcc gcaaagtgaa gacctgtaag caccagagca atagcgagta
     1081 tctatcactc cctgtagagt atctggtaga ccaagaggac acagaggacg accatataca
     1141 actcctgtaa gctcatggat gtactcctta tgttcactca gggtttatag ttactctgcg
     1201 gcagtaaaac gaataacatg gaccaacggg aaagaggcgt cttcctcaac gtcaaactca
     1261 cctgctttca ccttaacgga accagtagcc aacacatcga ccacactgat taactcaagg
     1321 tcatacccag cagtgttgct atttcgggtc actttgacat ctaccgtctt agcgtcttta
     1381 gttgacctga aagagataga agcgaaccgc ttaggcagga gatagacacc tgtatcatcg
     1441 cccgcatagc cgcatacagt gcagcccaat agctcactaa taccttcatg acctgcgcgt
     1501 tgagcggctc ggtccaagat tgcctcaagt ttctcaggga ccaatacagc gtaatcgctc
     1561 agtgtatttc ccaactcgac cggaagattc aacgctaatg tatccagaag atcttcagct
     1621 tgatcagacg gtttaccact tagtggggct gcctcaatga tttgaaggtc tgacgtatct
     1681 ttcaggacat cgaccacagc ggcgaacatc tggcggcagt attgaacctg catatcgcat
     1741 acagtctgga ttaatgcccc ctcggtccaa ccgcccatca tatcgaggtc agacacatga
     1801 gtagcagcag aacacaggat gccc
//
LOCUS       DAJTUN010000251         1821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTUN010000251 DAJTUN010000000
VERSION     DAJTUN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.257"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..185)
                     /locus_tag="OO470_003972"
     CDS             complement(<1..185)
                     /locus_tag="OO470_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6253031.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVKQLRDERQG"
     gene            371..1213
                     /locus_tag="OO470_003973"
     CDS             371..1213
                     /locus_tag="OO470_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6253032.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1216..>1821
                     /gene="capU"
                     /locus_tag="OO470_003974"
     CDS             1216..>1821
                     /gene="capU"
                     /locus_tag="OO470_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6253033.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
BASE COUNT          528 a          338 c          397 g          558 t
ORIGIN      
        1 ccctgccgct catcacgtaa ttgtttcacc catcgcgtca ttgtggaaag gccgacatcc
       61 atagcgctgg ctgcatctgc cacggtgtat ttctggtcaa cgaccagttg agcggattcg
      121 cgtttaaact ctgcgctgaa atttcttttt tcattatggc acctgtgttg ttctgaggtg
      181 agcatatcac ctctgttcag gtagctaaat ttagtgtgcc acctcaaggt tcttagtgag
      241 ccgtttttaa aagatatgat aatatattat tgtttataaa ttgttgatgt ttgatttagt
      301 agtatataaa gttcaccacc tttattaggc tttcctttta tctctactta ctgggtgcca
      361 gaaactttaa attttaaacg gagggggcat tttgtttaaa gcaaatcatg ttccggtatt
      421 aatgtatcat catgtcagcc attgcccagg actggttaca ctttctcccg agacattccg
      481 gaaacaaatg aaatggttgg ctgaaaacaa ctggaaaaca ctgtcatcag atgaactgga
      541 gtttttttat cggggcggaa aacttccccg taaaagcgtt atgttgacat ttgatgatgg
      601 ataccttgac aactggtttc aggtatatcc tttattaaac gagtttaatc tcaaggcaca
      661 tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc ctggaaagga
      721 atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata atgttatgct
      781 tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc atgttcatac
      841 acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt gtaaacatct
      901 tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa aatgcagtaa
      961 acatttgtgt tggccggaag gttattataa caaggattat attcagatcg ctgaagaact
     1021 tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta aaggaactac
     1081 acggatagga agaataagca ccaaggaacg ggaaaattgc gtatggctga aaagacgact
     1141 tttttattat acaacaccgt ttttttcatc gttgctggcc cttcataagg ggccaagatt
     1201 accggatgac taataatgaa tatactattt acggaatcat caccgaatat aggtggtcag
     1261 gaattacagg ctgttgctca aatgaaggcc ctgaagaaaa tggggcattc agttctgctt
     1321 gtctgcaggg agaacagcaa aattgctttt gaagccagta agtttggaat tgatatcaca
     1381 ttcgcgttat ttcgaaacag tcttcacatt cctactgtat ggagattact cggaatagtt
     1441 catagttttc agcccgatgc aatcgtttgt cacagtggac atgatagtaa tattgttggt
     1501 ttggtacggt tttttacctg gaaacatcca ttcagaatta tcaggcaaaa gacatatttg
     1561 acacgaaaaa caaaattttt ttcaataaat catttttgcg atgaggtgat tgttcccgga
     1621 acaaatatga agacacatct ggagcaggaa ggatgtcgaa ctcgggttac tgttgtgcct
     1681 cctggctttg atttccagga attatacgtt gattcccgaa acagtttgcc tccgagtgtt
     1741 ctttcctggc tggcgtcccg aagaggatgc cccgtaattg ctcaggtagg aatgttgcgc
     1801 ccggaaaaag ggcacgaatt t
//
LOCUS       DAJTUN010000252         1800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTUN010000252 DAJTUN010000000
VERSION     DAJTUN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.258"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..570
                     /locus_tag="OO470_003975"
     CDS             <1..570
                     /locus_tag="OO470_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6253034.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="OO470_003976"
     CDS             571..936
                     /locus_tag="OO470_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6253035.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="OO470_003977"
     CDS             933..1616
                     /locus_tag="OO470_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6253036.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          502 a          381 c          499 g          418 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
//
LOCUS       DAJTUN010000253         1789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTUN010000253 DAJTUN010000000
VERSION     DAJTUN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.259"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..783
                     /gene="phnC"
                     /locus_tag="OO470_003978"
     CDS             <1..783
                     /gene="phnC"
                     /locus_tag="OO470_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6253037.1"
                     /translation="TIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKSTL
                     LRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLSVL
                     ENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQRVA
                     IARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALRYC
                     ERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            808..>1789
                     /gene="phnD"
                     /locus_tag="OO470_003979"
     CDS             808..>1789
                     /gene="phnD"
                     /locus_tag="OO470_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6253038.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNAL"
BASE COUNT          441 a          528 c          479 g          341 t
ORIGIN      
        1 acgattatcc gtgtcgagaa gctcgccaaa accttcaatc agcatcaagc gctgcatgcg
       61 gttgatctga acattcatca cagtgaaatg gtggctctgc ttgggccgtc gggttccggc
      121 aaatccaccc ttttacgtca tttaagcggt ttgattaccg gcgataaatc cgccggtagc
      181 catatcgagc tactgggccg cactgtccag cgcgaaggcc gcctggcccg cgatatccgc
      241 aaaagccgcg ccaacaccgg ctacatcttt caacaattca acctggtgaa ccgcctgagc
      301 gtactggaga acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt
      361 agctggttca ccggcgagca gaaacagcgc gcgttacagg cgctgacccg cgttggcatg
      421 gtgcattttg cccatcagcg cgtttccacc ctctccggcg gccagcagca acgtgtggcg
      481 attgcccgtg cgctgatgca gcaggcgaaa gtgattctgg ccgatgaacc catcgcctcg
      541 ctggacccgg aatcagcgcg catcgtgatg gacaccctgc gcgacatcaa ccagaacgac
      601 ggcatcaccg tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc
      661 atcgtcgccc tgcgtcaggg gcacgtcttc tacgacggca gcagccaact gtttgataac
      721 gaacgttttg accatctcta ccgcagcatt aaccgcatcg aagagaacgc gaaagctgcc
      781 tgatatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc
      841 accagcatgt tcagcctcag caccctgtta accccggcac acgccgaaga gcaggaaaag
      901 gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg
      961 ccgtttttac aggatatgga gaagaaactg ggcgtgaagg tcaacgcctt ctttgccccg
     1021 gactacgcgg gcattatcca ggggatgcgc ttcaataaag tggatattgc ctggtacggc
     1081 aacctgtcgg cgatggaagc ggtggatcgc gccaatggcc aggtcttcgc ccagacggtc
     1141 gcggcggatg gatcgccggg ttactggagc gtgttgatcg tcaacaaaga cagcccgatc
     1201 aacaacctga acgatctgct ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct
     1261 aactccacct ctggcttcct cgtccccggt tactacgtct tcgctaaaaa caatatctcc
     1321 gccagcgact tcaagcgcac cgtcaacgcc gggcatgaaa ccaacgcgct ggccgtcgcc
     1381 aacaagcaag tggatgttgc caccaacaac accgaaaacc tcgacaagct gaaaacctcc
     1441 gcgccggaga agcttaaaga gctgaaggtg atctggaaat cgccgctgat cctaggcgat
     1501 ccgatcgtct ggcgcaagaa tctgtccgaa actaccaaag acaagatcta cgacttcttt
     1561 atgaactacg gaaaaacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggcgccg
     1621 ttccgcgctt ccagcgacct gcaactggtg ccgattcgcc agctcgcact gtttaaagag
     1681 atacaaagcg tgaaaggcaa taaagggctg aatgagcagg acaagctggc gaaaaccacc
     1741 gagattcagg cgcagctgga tgacctggac cgcctgaaca acgcgttaa
//
LOCUS       DAJTUN010000254         1777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTUN010000254 DAJTUN010000000
VERSION     DAJTUN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.260"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..213
                     /locus_tag="OO470_003980"
     CDS             <1..213
                     /locus_tag="OO470_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR6253039.1"
                     /translation="ALLDWLIAARPTVTDQALHEHNNVSYQQHIAIVDAIRRHDPDEA
                     DRALQSHLNSVSATWHAFGQTTNKKK"
     gene            335..1228
                     /gene="nanA"
                     /locus_tag="OO470_003981"
     CDS             335..1228
                     /gene="nanA"
                     /locus_tag="OO470_003981"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6253040.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            1338..>1777
                     /gene="nanT"
                     /locus_tag="OO470_003982"
     CDS             1338..>1777
                     /gene="nanT"
                     /locus_tag="OO470_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6253041.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASG"
BASE COUNT          412 a          463 c          469 g          433 t
ORIGIN      
        1 gccctgctcg actggcttat tgccgcacgc ccaacggtta ccgatcaggc actgcacgaa
       61 cataacaacg ttagttatca acagcatatt gcgatcgttg atgcgatccg ccgtcatgat
      121 cctgacgaag ccgatcgtgc gttgcaatcg catctcaaca gcgtctctgc tacctggcac
      181 gctttcggtc agaccaccaa caaaaagaaa taatgccact ttagtgaagc agatcgcatt
      241 ataagctttc tgtatggggt gttgcttaat tgatctggta taacaggtat aaaggtatat
      301 cgtttatcag acaagcatca cttcagaggt atttatggca acgaatttac gtggcgtaat
      361 ggctgcactc ctgactcctt ttgatcaaca acaagcactg gataaagcga gtctgcgccg
      421 cctggttcag ttcaatattc agcagggcat cgacggttta tacgtgggtg gttcgaccgg
      481 cgaagccttt gtacaaagcc tttccgagcg tgaacaggta ctggaaatcg tcgccgaaga
      541 ggcgaaaggt aagattaaac tcatcgccca cgtcggttgc gtcagcaccg ccgaaagcca
      601 acaacttgcg gcatcggcta aacgttatgg cttcgatgcc gtctccgccg tcacgccgtt
      661 ctactatcct ttcagctttg aagaacactg cgatcactat cgggcaatta ttgattcagc
      721 ggatggtttg ccgatggtgg tgtacaacat tccagccctg agtggggtaa aactgaccct
      781 ggatcagatc aacaccctgg ttacgttgcc tggcgtcggt gcgctgaaac agacttctgg
      841 cgatctctat cagatggaac agatccgtcg tgaacatcct gatctggtgc tctataacgg
      901 ttacgacgaa atcttcgctt ccggcttgct ggcaggcgct gatggtggta tcggcagtac
      961 ttacaacatc atgggctggc gatatcaggg gatcgtgaag gcgctgaaag aaggcgatat
     1021 ccagaccgcg cagaaactgc aaactgagtg caacaaagtc attgatttac tgatcaaaac
     1081 gggcgtattc cgcggcctga aaactgtcct gcattatatg gatgtcgttt ctgtgccgct
     1141 gtgccgcaaa ccgtttggac cggtagatga aaaatatctg ccagaactga aggcgctggc
     1201 ccagcagttg atgcaagagc gcgggtgagt tgttttcccc tcgctcgccc ctaccgggtg
     1261 aggggaaata aacgcatctg taccctacaa ttttcatacc aaagcgtgtg ggcatcgccc
     1321 accgcgggag actcacaatg agtactacaa cccagaatat cccgtggtat cgccatctca
     1381 accgtgcaca atggcgcgca ttttccgctg cctggttggg atatctgctt gacggttttg
     1441 atttcgtttt aatcgccctg gtactcaccg aagtacaggg tgaattcggg ctgacgacgg
     1501 tgcaggcggc aagtctgatc tccgcagcct ttatctctcg ctggttcggc ggcctgatgc
     1561 tcggcgctat gggtgaccgc tacgggcgtc gtctggcaat ggtcaccagc atcgttctct
     1621 tctcggccgg gacgctggcc tgcggctttg cgccaggcta catcaccatg tttatcgctc
     1681 gtctggtcat cggcatgggg atggcgggtg aatacggttc cagcgccacc tatgtcattg
     1741 aaagctggcc aaaacatctg cgtaacaaag ccagtgg
//
LOCUS       DAJTUN010000255         1753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTUN010000255 DAJTUN010000000
VERSION     DAJTUN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.261"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>1753
                     /gene="sat"
                     /locus_tag="OO470_003983"
     CDS             <1..>1753
                     /gene="sat"
                     /locus_tag="OO470_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR6253042.1"
                     /translation="NADTTGTQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGH
                     EYNINGQGFTFKGAGIDIGKESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGE
                     GNVILNEEGTFNNIYLASGNGKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFT
                     RIAATDDGTTITNSDTTKEAVLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLI
                     LDGSVNTKNDVEVSNASLTMQGHATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSY
                     NKKFNSDYKSNNQQTSFDQPDWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAI
                     TIGDKNAYIDNLAGKNITNNGFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVV
                     TLNGATFLNNTPISIDKGAKVIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHY
                     AADGFRLSGGNANFIARNMASVTGNIYADDAATITLGQPETETPTISSAYQAWAETLL
                     YGFDTAYRGAITAPKATVSMNNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVD
                     NLTIDDSAFVLRANLAQA"
BASE COUNT          628 a          315 c          353 g          457 t
ORIGIN      
        1 aatgcagata ccactggtac tcaagaaaaa ataactaaag acaaagatat tgtgttcaca
       61 aatgggggaa atgttctgtt taaggataat ttggattttg gtagcggtgg tattatcttt
      121 gacgaaggcc atgaatataa cataaacggt cagggattta catttaaagg agcaggaatt
      181 gatatcggaa aagaaagcat tgtaaactgg aatgcattgt attccagtga tgatgtttta
      241 cacaaaatag gccccggtac tctgaatgtt caaaaaaaac agggggcaaa tataaagata
      301 ggtgaaggaa atgttattct taatgaagaa ggaacattta acaatatata ccttgcaagc
      361 ggaaatggta aggtaatact aaataaagat aattcccttg gcaatgatca atatgcgggg
      421 atatttttta ctaaacgtgg tggcacgcta gatttaaatg gacacaatca gacttttact
      481 agaattgccg ccactgacga tggaacaaca ataactaact cagatacaac gaaagaagcc
      541 gttctggcaa tcaataacga agactcctac atatatcatg ggaacataaa tggcaatata
      601 aaactaacgc acaatattaa ttctcaggat aagaaaacta atgcaaaatt aattctggat
      661 ggtagtgtca acacaaaaaa tgatgttgaa gtcagtaatg ccagtcttac catgcaaggc
      721 catgcaacag agcatgcaat attcagaagc acagcgagtc attgctccct ggtatttctt
      781 tgtggaacgg actgggtcac cgttttgaaa gaaacagaga gttcatataa taaaaagttc
      841 aattctgatt acaaaagtaa taatcagcag acctcatttg atcagcctga ctggaaaacc
      901 ggggtgttta aatttgatac attacacctg aacaatgctg acttttcaat atcacgcaat
      961 gccaatgttg aaggaaatat atcagcaaat aaatcagcta tcacaatcgg cgataaaaat
     1021 gcttacattg ataatcttgc agggaaaaat attactaata atggttttga cttcaaacaa
     1081 actatcagta ctaatctatc cataggagaa actaaattta caggtggcat cactgcacat
     1141 aacagccaaa tagccatagg tgatcaagct gtagttacac ttaatggtgc aacctttctg
     1201 aataatactc ctataagtat agataaagga gcaaaagtta tagcacaaaa ttccatgttc
     1261 acaacaaaag gtattgatat ctccggtgaa ctgactatga tgggaatccc tgaacagaat
     1321 agtaaaactg taacgccggg tctccactac gctgctgatg gattcaggct gagtggtgga
     1381 aatgcaaatt tcattgccag aaatatggca tctgtcaccg gaaatattta tgctgatgat
     1441 gcagcaacca ttactctggg acagcctgaa actgaaacac cgactatatc gtctgcttat
     1501 caggcatggg cagagactct tttgtatggc tttgataccg cctatcgagg cgcaataaca
     1561 gcccccaaag ctacagttag catgaataat gcgatctggc atctaaatag ccagtcatca
     1621 attaatcgtc tagaaacaaa agacagtatg gtgcgtttta ctggtgacaa tgggaagttt
     1681 acaaccctta cagtggacaa ccttactata gatgacagtg catttgtgct gcgtgcaaat
     1741 ctggcccaag cag
//
LOCUS       DAJTUN010000256         1734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTUN010000256 DAJTUN010000000
VERSION     DAJTUN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.262"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..969)
                     /gene="kdpA"
                     /locus_tag="OO470_003984"
     CDS             complement(<1..969)
                     /gene="kdpA"
                     /locus_tag="OO470_003984"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCR6253043.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGK"
     gene            complement(969..1058)
                     /gene="kdpF"
                     /locus_tag="OO470_003985"
     CDS             complement(969..1058)
                     /gene="kdpF"
                     /locus_tag="OO470_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6253044.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1371..1577
                     /locus_tag="OO470_003986"
     CDS             1371..1577
                     /locus_tag="OO470_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6253045.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          497 a          433 c          408 g          396 t
ORIGIN      
        1 tttaccttcc atattgatgc tgctgtctgc gccgagcgcc agcagatgag gattaccctg
       61 aacttctgcc cacataacca cgcctacgca gatgacaaaa atcactgaca tcgcccacag
      121 caacatgcgc ccctggcggc gatcgcccgt cacttcacca aaggcaaagc acagcgctgt
      181 tgggatcaag aagatcgcca gcatctgcac gaagttggtc agtgcggttg ggttttcaaa
      241 cggatgcgac gagttggcat taaagaagcc accgccgtta gtaccgagca tcttgatcgc
      301 ttcctgagaa gctactggtc ccatgggtaa cagctgttgc gctccttcaa cggtattcac
      361 tgcctgatag ggcagaaagt tttgctgcgc accttgttga ataaaaaaca gtgcgatcaa
      421 caacgccaca gggactagca cccataacgt gatgcgtagc agatcgaccc aggcattccc
      481 gagtgtgctc atgctctggc gagtaaacgc acggatgaag gcaaaaatca ccgcaatccc
      541 gctggcggca gaaagaaagt tttgtaccgt taagcctgcc atctggctga aatagctcaa
      601 cgtggtttca ccgctataag attgccagtt ggtattcgtg acaaagctga cggcggtatt
      661 cagcgccaga tcccacgaca gccccggcaa ctgctgtgga ttaagcggca gatagtgctg
      721 accgagcaac ataaaaaaca gcaccgccag ccccagcatg ttcaggccga gaatggcaca
      781 aagatattgc ttccagttca tctcatggtc agagacgcca ggtaaacgaa agagtatacg
      841 ttcaacgccc gctgtacctg gaagaggaat gtcattaatc agccgcgcca gcccgctgcc
      901 taaaggacgc gccagcacca ttaataccag taaaaacgtg gcaatcagta agaacccttg
      961 cgcagccatc agaacgcctc cgcattgatc agggcataaa ccaaataacc cagtaataaa
     1021 aacaccagca acacgccggc tatcacgcct gcactcacag tgcacctcca gtggcctaaa
     1081 agtgataccg gaagagtaga attctggctg caaagatttc gcaaaaatct gcggacgggg
     1141 tgtaaaaaaa gtataaaaat ggcaaaggcc atgatttaac tgatggttag tattaattta
     1201 acttttgtgt aatttaatta cagtgtgaat gtaaataaac catcaataag caaaaataag
     1261 tggtcggatg agtagtaaaa ttacacaaaa ggcggtacta ttttcatcag ataaacaaat
     1321 tcatttttcc ggtgctattc accgggtaga tacaaaaggg ggagaaaagt atggaactct
     1381 acagagaata tcctgcatgg cttatctttt tacgccgtac atatgcggtt gcagcgggcg
     1441 ttctggcgct gcctttcatg cttttctgga aagaccgcgc ccgcttttac agctacctgc
     1501 accgcgtctg gtcgaaaacc agcgataaac cggtgtggat ggatcaggcc gaaaaggcaa
     1561 ccggtgattt ttactgattg cctgcgcgct taagccagcc tcgtatcagg gggctggttt
     1621 aggtgttcaa catcaacgtc ttccatgata attaacatgg aagacgtttc catcgtatta
     1681 ttcatttcat cactgtttta gcatcacgtt tcaatagtat cctttcatag ggct
//
LOCUS       DAJTUN010000257         1708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTUN010000257 DAJTUN010000000
VERSION     DAJTUN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.263"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..486
                     /gene="ssuA"
                     /locus_tag="OO470_003987"
     CDS             <1..486
                     /gene="ssuA"
                     /locus_tag="OO470_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6253046.1"
                     /translation="QPTYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDG
                     TDLNQTGSFYLAARPYAEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAP
                     VIASYLDHRPPTTIKPVNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQ
                     L"
     gene            483..1628
                     /gene="ssuD"
                     /locus_tag="OO470_003988"
     CDS             483..1628
                     /gene="ssuD"
                     /locus_tag="OO470_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6253047.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          376 a          474 c          487 g          371 t
ORIGIN      
        1 cagcccactt acctgacgcc tgccgatgcc cgcgccgcgt tccagcaagg taacgttgac
       61 gcctgggcta tctgggatcc ctactactcc gctgcgttat tacagggcgg cgtgcgggtg
      121 ctgaaagacg gcaccgatct caatcaaact ggatcgtttt atctggcagc tcgtccctat
      181 gcagaaaaaa acggcgcttt tattcagggc gtactggcaa cctttagtga ggccgatgcg
      241 ttaacccgca gccagcgcga acaaagcatc gctttactgg caaaaacgat gggcttaccg
      301 gcaccggtga ttgcctctta cttagatcat cgccctccta ccaccatcaa accggttaac
      361 gccgaggttg ccgccttaca gcagcaaacg gcagatctgt tttatgaaaa ccgtctggtg
      421 ccgaaaaaag tcgatattcg ccagcgcatc tggcagccca ctcaactgga aggaaaacaa
      481 ttatgagtct gaatatgttc tggtttttac cgacccacgg tgacgggcac tatctgggaa
      541 cggaagaagg ttcacgcccg gttgatcacg gttatctgca acaaattgcg caagcggcgg
      601 atcgtctggg ctataccggt gtgctgatcc caacggggcg atcgtgtgaa gatgcgtggc
      661 tggttgcagc gtcgatgatc ccggtgacgc agcggctgaa gtttcttgtc gccctgcgtc
      721 ccagcgtaac ctcacctact gttgccgccc gccaggccgc cacgcttgac cgtctctcaa
      781 atggtcgtgc gttgtttaat ctggtcacag gcagcgatcc acaagagctg gcaggcgacg
      841 gcgtgttcct tgatcatagc gagcgctacg aagcctcggc ggaatttacc caggtctggc
      901 ggcgcttatt gctgggggaa accgtcaatt tcaacggtaa acatattcat gtgcgcggag
      961 caaaactgct cttcccgccg attcaacagc cttatcctcc gctttacttt ggcggatcgt
     1021 cagatgtcgc ccaggagctg gcggcagaac aagttgatct ctacctcacc tggggcgaac
     1081 cgccggaact tgttaaagag aaaatcgaac aagtgcgggc gaaagctgcc gcgtatgggc
     1141 gcaaaattcg tttcggtatt cgtctgcatg tgattgttcg tgaaactaac gacgaagcgt
     1201 ggcaggccgc cgagcggtta atctcgcatc ttgatgatga gactatcgcc aaagcacaag
     1261 ccgcattcgc ccggacggat tccgtaggac aacagcgaat ggcggcgcta cataacggca
     1321 agcgcgacaa tctggagatc agccccaatt tatgggcggg cgttggctta gtgcgcggcg
     1381 gtgccggaac ggcgctggtg ggcgatggtc ctacggtcgc cgcgcgaatc aacgaatacg
     1441 ccgcgcttgg catcgacagc tttgtgcttt cgggctaccc gcatctggaa gaagcgtatc
     1501 gggttggcga gttgctgttc ccacatctgg atgtcgccat tccggaaatt ccccagccgc
     1561 agccgctgaa tccgcaaggc gaagcggtgg caaatgattt tatcccccgt aaagtcgcgc
     1621 aaagctaagg aacacgatga tggcaacgcc agtgaagaag tggttattgc gcgttgcccc
     1681 ctggttttta ccggtgggca tcgtggcg
//
LOCUS       DAJTUN010000258         1682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTUN010000258 DAJTUN010000000
VERSION     DAJTUN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.264"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..605
                     /locus_tag="OO470_003989"
     CDS             <1..605
                     /locus_tag="OO470_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253048.1"
                     /translation="FNHLENEITELNKKLSALVSSEDENKRRDEHYAAIYDYCHKVAH
                     ETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAPDKDQEPHRP
                     FRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIGRCMGYLQVA
                     AEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            671..1204
                     /locus_tag="OO470_003990"
     CDS             671..1204
                     /locus_tag="OO470_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6253049.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1201..1671
                     /locus_tag="OO470_003991"
     CDS             1201..1671
                     /locus_tag="OO470_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6253050.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
BASE COUNT          445 a          380 c          457 g          400 t
ORIGIN      
        1 gattcaatca cctggaaaat gaaatcactg agcttaataa aaaattgtcg gcgttggtga
       61 gttctgaaga tgaaaacaaa cgccgcgatg agcattatgc ggcgatttac gattactgcc
      121 acaaagtcgc tcacgagact tttatgaagt ttttgcagga aaagttttta ccggccgcat
      181 tgtcagaaaa agaggcggct tacctgcggc ctgagtacgt cattacggtt aacggtgccg
      241 gagaggaaga gcataaaagt gattttattg cgtctgcacc ggataaagac caggaacccc
      301 atcggccttt cagggtttcc tgtgaagagg gtgagttcgt cgtttatgag aacggaaaac
      361 ctgtccgggc atcgcatcat cactgcctga aaattattaa tctcgctatc agatgcctga
      421 aagacgaaaa caccagagtt atgaaacgca ttggccgttg catggggtat ttgcaggtgg
      481 cggcagagat tgaagcactt gccagtggtg cagacatgga tgctgcggtg cgggaggctc
      541 ttcttcgtga tttcaatact ccccctttaa gaaagagcct gatgactgga tccagccggg
      601 gctgacttat cttaaaaggc gtatataagt tggctcgtta tttgttgccg ataaatcctg
      661 ataaatatcc atgagcgcaa aaatcaaata cggcctgtcg gctgctgttc tggcgctgat
      721 tgccgcaggc gcgtctgctc cccagatact tgaccagttt ctgaacgaaa aagagggtaa
      781 tcacacaacg gcataccgtg atggttctgg catatggacc atctgtcgtg gtgccacaat
      841 ggtggatggt aagcctgtca taccgggaat gaagctgtcg aaggaaaaat gtgaccaggt
      901 taacgctatt gaacgtgata aggcgctggc atgggtggag cgcaatatta aagtacctct
      961 gaccgaaccc cagaaagcgg gtatagcgtc attttgcccc tataacattg gccccggtaa
     1021 gtgtttcccg tcgacgtttt ataagcggct gaatgccggt gatcgtaagg gcgcatgcga
     1081 ggcgattcgc tggtggataa aagatgttgg gcgcgattgc cgcatacgtt caaataactg
     1141 ctatggacag gttattcgtc gtgaccagga aagcgcatta gcctgttggg ggatagatca
     1201 atgagcagag tcaccgcgat tatctccgct ctggttatct gcatcatcgt ctgcctgtca
     1261 tgggctgtta atcattaccg tgataacgcc attacctaca aagagcagcg cgacaaaaac
     1321 gccggagaac tgaagctggc gaacgcaacc attactgaca tgcagcagcg ccagcgtgat
     1381 gctgatgcac tcgatgctaa atacacgaag gagttagctg atgcgaaagc tgaaaatgat
     1441 gctcttcggc gcaagcttga taatggtggt cgggtgctcg tcaaaggaaa atgccctgtg
     1501 ccatcctcag ccgaaacctc cagcgcctcc ggcatgggca atgatgccac cgtcgaactc
     1561 tctccagttg ctggacgaaa cgttctcggt atccgggacg gaatcatcag cgaccagaca
     1621 gcattgagaa tgcttcagga gtacatcagg atccaatgcc ttggggggta gcggtaattt
     1681 ta
//
LOCUS       DAJTUN010000259         1623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTUN010000259 DAJTUN010000000
VERSION     DAJTUN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.265"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..734
                     /locus_tag="OO470_003992"
                     /pseudo
     CDS             1..734
                     /locus_tag="OO470_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            731..>1623
                     /locus_tag="OO470_003993"
     CDS             731..>1623
                     /locus_tag="OO470_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6253051.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          438 a          374 c          405 g          406 t
ORIGIN      
        1 atgccgaccc ggacaccttt tccgtcgtgc gctggatgcc cggctcactc ttgacctggc
       61 gtgataaaaa cgggctgcag cgtaaagtcc tcgacaagga aaatctggcg tgggatgaag
      121 atttaacaaa gcactgtctg gatttttctc tgctggaaaa gaaagtgttc tggcgtaaag
      181 gacctgcttg taaacaggaa gaattacccg gactcgatcc ggagcagttt caacccatca
      241 gtgatgctgt cgcccagcat caggactcgc tttataccat catcgaaaca gagtctggtg
      301 accgtaagct ggagatcgtg aaacttgatg atcccaatct tattatcaac aaacgtttca
      361 acgccgggaa acgccacggc tatttactta cgcgtgccga agggtagcca taccattccg
      421 gtttacacgt gtttgaatct gacggaccgc tgatcttact ggataaccgt tctccggatg
      481 aacgcgaagc ccatcttaat gaccatccct ttttgcgcag atggtatgcc cgcgataacc
      541 gctacattta cagctttgat ggcgcgcagc tctggcgata ccgcaccgct gatccgaaac
      601 aagttcgctt aatctggaag gaacaacatt cgggatatgt ctatggcgta aattacaaaa
      661 cgggatatct ggacggaaaa attactgatg acggcgaatt tattcctgcc ccgcgcaatg
      721 aggcgacaaa atgaagggtc aaataatcac agttgcactc atcctgttgg gacacacatt
      781 tctgacgcct gctgtacagg ccatagggtt cgactattac aatgatcacg gtgttatgtc
      841 ttacggtaaa ggttattggg gggataagaa aatcattgcc cagtttccgg agatgagcag
      901 ggccggtttg cgccttgtga aaaatatctc tgggaaaacg ggttatatac cttccgatga
      961 aataagcata gaaaatgagg aataccactg ggtgacagac ggccgcgtta ttctctggcg
     1021 tggcaaaatc gttagcaatc caccgggaac actcactgtc gatattgcca gctttcaggc
     1081 tatgggccgc ttcgcggtcg ataaatatag cctctatttt gacggacagc gcaccgaaag
     1141 taatagcggc gcgtcacgtg ttgagctggc gacactaaaa gctatcgaag gtaactctac
     1201 cacgctggtg gatagtaaaa atctctactt gtccggtcga cgtcagggga gcagcagtga
     1261 tgttactgta ttagaaaaaa gatggtgggg tattaatcca cgtcttatga gtgtaaacag
     1321 aaattcatat tccaatgatc tgcttatccg cagtgggcag aatatttatt taaatggcgt
     1381 tcaccttacg gcgaatgcag actcatttga gataattcgc tggatacctc attcactgct
     1441 ggtttttcgc gacaataagg gtctgcatcg ttatcccttt ggtcaattat caggcaaagc
     1501 gatacccgta gatgatgacg tctcttttga agtaggggaa agtcgcgttc gctggcgtaa
     1561 acagctcacg cccgaccgtc agtggagcaa gtggatagac ctaccaggta ttgaacctga
     1621 aca
//
LOCUS       DAJTUN010000260         1589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTUN010000260 DAJTUN010000000
VERSION     DAJTUN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.266"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..718
                     /gene="narZ"
                     /locus_tag="OO470_003994"
     CDS             <1..718
                     /gene="narZ"
                     /locus_tag="OO470_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="with NarYV catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli;
                     expression of nitrate reductase Z is not dependent on
                     nitrate levels; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6253052.1"
                     /translation="SPTWSGLESDHVSYNAGYTNVHELIPWRTLSGRQQLYQDHPWMR
                     AFGESLVAYRPPIDTRSVSEMRQIPPNGFQEKALNFLTPHQKWGIHSTYSENLLMLTL
                     SRGGPIVWISETDARELTIVDNDWVEVFNANGALTARAVVSQRVPPGMTMMYHAQERI
                     MNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQLAWGFNYYGTVGSNRDEFIMIRK
                     MKNVNWLDDEGRDQVQEAKK"
     gene            715..>1589
                     /gene="narH"
                     /locus_tag="OO470_003995"
     CDS             715..>1589
                     /gene="narH"
                     /locus_tag="OO470_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6253053.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT          404 a          424 c          440 g          321 t
ORIGIN      
        1 cagccccaca tggtccggtc tggaaagcga tcacgtatcc tataacgcgg gatacaccaa
       61 cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct atcaggatca
      121 tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta tcgacacccg
      181 tagcgtcagt gagatgcgcc agataccgcc aaacggcttc caggaaaaag cacttaactt
      241 cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc tgctaatgct
      301 gacgctctct cgcggtggac cgattgtctg gatcagcgaa acagatgccc gagaactgac
      361 cattgtcgat aacgactggg tggaagtatt taacgccaac ggcgcgctga ctgcccgcgc
      421 ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc aggaacgcat
      481 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata actcggtcac
      541 ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg cctggggctt
      601 taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc gcaagatgaa
      661 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
      721 atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg ccatacctgt
      781 tcggtgacct gtaacaacgt ctggaccgga cgcgaaggca tggagtacgc atggtttaac
      841 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
      901 caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg cagcaagatg
      961 ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga tgattactac
     1021 gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa acatatccct
     1081 actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat ctggggccca
     1141 aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag
     1201 gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta cctgccgcgc
     1261 ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg cgctatctac
     1321 aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg
     1381 tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa
     1441 aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc
     1501 tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg catcgaggaa
     1561 gcgtcgagca ccgagcgcga agttgacct
//
LOCUS       DAJTUN010000261         1573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTUN010000261 DAJTUN010000000
VERSION     DAJTUN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.267"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            19..969
                     /gene="virK"
                     /locus_tag="OO470_003996"
     CDS             19..969
                     /gene="virK"
                     /locus_tag="OO470_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6253054.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1034..>1573
                     /locus_tag="OO470_003997"
     CDS             1034..>1573
                     /locus_tag="OO470_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6253055.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGG"
BASE COUNT          455 a          293 c          351 g          474 t
ORIGIN      
        1 cgaagcaaaa gtaacaatat gttttctata agtaacttat catttatcgg tttccttaaa
       61 aggattattt tttcctcaga ttcactaccg gggaagtggg aacacagaaa atttcggttc
      121 atgtacattt tgcgatgttc tataaatccg gttgtcagta ttcgatatta ttatgaactg
      181 cgttccttgc cgtgcattga ggatattctg gcaatacacc ccacgttgcc agcaagaatt
      241 catcgccctt atttgcataa aggggggcgc gcctggaccc gtggacaata tattcttgag
      301 cattatcgct ttgtgcaaaa tttgccagaa aaatattcta aatttttgtt tccacaaaaa
      361 tcggtatcat tagtccaatt catcgggaaa gatggagaga attttgatat ccagtgttct
      421 cccagtggtt ttgaccggga aggagagttg atgttgtcat tgttttacaa taaaacagtt
      481 attgcccggt taacattttc agtcattctg actcaaaatg gacacattgc ttttattggc
      541 ggattacagg gagcccctaa aaataccgga cctgatgtta ttcggtgcgc aacccgggcc
      601 tgttatggga ttttcccaaa acggattatt tttgaagcat tttgtgcgtt gatgaaagct
      661 tgcaatatct ctgagtgtct ggcagtcagt gagcacagtc atgttttccg gcaattgaga
      721 tactggtatc agaaacgcaa aacctttgtt gcggtctata gtgatttctg ggagtctgta
      781 gcaggaaaaa cctgtggtga ctggtacaga ttaccaaccc aggtgattcg aaagccgcta
      841 agcgatatag ccagtaaaaa acgctctgag taccgaaaac gatacgcttt gctggattat
      901 atccatgaaa ctactatccg ctctttggat gcatatcctg tacactcaga acatcagaat
      961 ttaaattaag gtttgatgta ttctctgaat aaaacagtaa tgataatttt ggtagaggca
     1021 ttcgcactaa ataatgaaaa aatataaatc tgagtttatt cctgaattta agaaaaatta
     1081 cctttcccct gtttactggt ctacatggtt ccttttggga atgattgcag gtatttcaat
     1141 gtttccccct tcattcagag atcctgtctt ggcaaaaata gggcgttggg cgggcagatt
     1201 gagcaaaaaa gctcgtcgca gggcgacgat taatttatcg ctttgtttcc cagaaaagag
     1261 tgatacagaa cgggaaataa ttgtcgacaa aatgtttgtc acagcattgc aatccatagt
     1321 gatgatggca gaactggcaa ttcgtggtcc ggaaaagttc cagaaacgag tgttctggaa
     1381 aggcctcgaa attcttgagg aaattcgaca caataataga aatgtgattt ttctggttcc
     1441 ccatggctgg agcgtggata ttcctgcaat gttgctggca gctcagggaa aaaaaatggc
     1501 cgccatgttt catcagcaac gaaatccagt gattgattat gtctggaatt cagtacggcg
     1561 taaattcggg ggg
//
LOCUS       DAJTUN010000262         1545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTUN010000262 DAJTUN010000000
VERSION     DAJTUN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.268"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..542)
                     /locus_tag="OO470_003998"
     CDS             complement(<1..542)
                     /locus_tag="OO470_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6253056.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSCQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAV"
     gene            complement(539..1363)
                     /gene="dapF"
                     /locus_tag="OO470_003999"
     CDS             complement(539..1363)
                     /gene="dapF"
                     /locus_tag="OO470_003999"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6253057.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(1400..>1545)
                     /locus_tag="OO470_004000"
     CDS             complement(1400..>1545)
                     /locus_tag="OO470_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253058.1"
                     /translation="GLKGPLYFPPADKNAPPPTKPVETQTQSTVPDKNDRATGDGPSQ
                     VNY"
BASE COUNT          360 a          463 c          396 g          326 t
ORIGIN      
        1 accgctttcg cttccggtag caccaccagc agctccggtc cgttaagcgg cccaagatag
       61 tgctgttcct gccccaaacg ctgaatacgc agcggttcga aagactgaca gcttaatgcc
      121 agatgagtgt ggttcgacgg cgcacctaag cgccagcgat ctggaaacag gcgcagactc
      181 gcaccagcta ggccgagatc gcgcgcccag cggtgaaagc gcatcagcat atcgtcgaga
      241 ctgctggcgg cggtgagact gcgctgcagg tagagtaggc gataaaacag cccttcgttg
      301 gcgatagcct gctccatcaa caacgccatg ttctcttcca gaacatgaat gtgattacgt
      361 gcgcgggcca tgtgccactc gaccaacgaa acggtgccgc gtaccggatg cggcacacgt
      421 atcgcttcta ctgcgcgcgc attgcggata aaaaactcag gatttttaat cagataatcg
      481 acaaccgccc ggtcatcaag ctccgtgagt gtttcctgca gttcttcccc tggttgcttc
      541 atagatgaat aaatccatcg tagacatgta ccgccgggcc agtcatatat aacgggtgac
      601 ccggaccttt ccaggcgata tcaagacgac cgccggggag ttccacgcgt acttcttcgt
      661 ccagcaaacc ttgctgaatc cctaccgcca ccgccgcaca cgcgccgctg ccgcaggcct
      721 gggtttctcc tgccccacgc tcataaacgc gtaaacgaat atgctcgcgc ttaaccactt
      781 gcataaaacc gatattggcg cgctccggaa aacgctcgtg actttccaga acaggaccaa
      841 gcgtttctac cgccgcggta tcgacatcat cgacctgaat cacgcaatgc ggatttccca
      901 tcgacaccac gccgcataag attgtctgct cggcggcgcg cataatatag gtcttttccg
      961 ctttgttagc gcgaaacggc acggcggaag gttcgaagtt gggttcgccc atatttacgc
     1021 ggaccagatc atcatcggtg acggtcagaa ccatccgccc gttggcggtg ctgacgcgga
     1081 tatcacgctt attggtcagt cctttcagac gcacaaaacg ggcaaagcag cgcgcaccgt
     1141 taccgcactg cgccacttca ctgccatcag cattgaaaat gcgatagtga aaatccagtt
     1201 caggatcata cggcggctca accaccagca gctggtcaaa ccctaccccc aggtgccgat
     1261 ccgccaggcg acgaatcagc tccggtgaaa aaaagacatt ctgcgttacc gcgtcgacga
     1321 ccataaaatc gttgccaagg ccatgcattt tcgagaactg catcatttac tccaatcacg
     1381 cgggtacaga aactgacttt tagtaattca cctgggatgg accatcgcca gtggcgcgat
     1441 cgtttttatc cggcaccgtg gattgcgttt gcgtctctac cggtttggtc ggcggcggtg
     1501 cgtttttatc tgcaggcggg aaatagagcg gacctttcag accgc
//
LOCUS       DAJTUN010000263         1538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTUN010000263 DAJTUN010000000
VERSION     DAJTUN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.269"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(910..1386)
                     /locus_tag="OO470_004001"
     CDS             complement(910..1386)
                     /locus_tag="OO470_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6253059.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          410 c          385 g          395 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAJTUN010000264         1530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTUN010000264 DAJTUN010000000
VERSION     DAJTUN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.270"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            166..>1530
                     /locus_tag="OO470_004002"
     CDS             166..>1530
                     /locus_tag="OO470_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6253060.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQY"
BASE COUNT          415 a          364 c          396 g          355 t
ORIGIN      
        1 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt
       61 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
      121 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
      181 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
      241 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
      301 gttagtcctt tattaacatc cgctggcata aatgctggcc agttgcgcac agatatcaat
      361 caggcattaa atcgtttacc gcaggttgaa ggtactggtg gtgatgtcca gccatcacag
      421 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt
      481 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
      541 ctgaaagcaa caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
      601 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
      661 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
      721 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
      781 ggtgaacccg gcgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
      841 ggcgaagtgc cggaaggatt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
      901 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
      961 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggc
     1021 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     1081 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     1141 gatgctgcgc tggaacgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagat
     1201 accattgcga ttctgcgtgg cctgaaagaa cgttacgaat tgcaccacca tgtgcaaatt
     1261 actgacccgg caattgttgc agcagcgacg ttgtctcatc gctacattgc tgaccgtcag
     1321 ctgccggata aagccatcga cctgatcgat gaagcagcat ccagcattcg tatgcagatt
     1381 gactcaaaac cagaagaact cgaccgactc gatcgtcgta tcatccagct caaactggaa
     1441 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     1501 gaagaactga gcgacaaaga acgtcagtac
//
LOCUS       DAJTUN010000265         1485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTUN010000265 DAJTUN010000000
VERSION     DAJTUN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.271"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(90..>1485)
                     /gene="kup"
                     /locus_tag="OO470_004003"
     CDS             complement(90..>1485)
                     /gene="kup"
                     /locus_tag="OO470_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6253061.1"
                     /translation="KHGTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMW
                     AVHFFLEYKTVSFIALGAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYF
                     GQGALLLKNPEAIKNPFFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRL
                     GYLSPMRIIHTSEMESGQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMV
                     LTSILSTTVARQNWHWNKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMF
                     IVMTTWKSERFRLLRRMHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALM
                     HNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEE
                     VFHRCGLEGLSCRMMETSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEI
                     PPNRVIELGTQVEI"
BASE COUNT          417 a          408 c          362 g          298 t
ORIGIN      
        1 cggaaatgtg atcaacctcg aaacgtttta catggtgatt aaccatgaaa acaaaaacgc
       61 ccccttatga agaaaggagg cgtctggcgt tagatttcga cctgagtacc cagttcgata
      121 accctgtttg gcgggatttc aaattgatct ggtgcacgca gcgcattacg ttgcagcagc
      181 aagtacagct tgccgcgcag acgcaaatac cacgggcgtt tgccgaggat caacgactca
      241 tgcgacataa agaaggaggt ttccatcatc cggcaactta atccttccag accgcagcgg
      301 tggaaaactt cttccacgtt tggcgtttct cgccaaccat aacttgccac cacgcgccag
      361 aaagtgggcg acagttgttc aatctgtacc cgacggacgt tatggacata tggcgcgtct
      421 tcggtgcgca gagttaacag aatcacccgc tcatgcaata ccttgttatg tttaaggtta
      481 tgcatcagcg caaagggaat gacgttgatt gcacgcgaca tatacaccgc ggtcccgggc
      541 acgcgaacag gcggtgattt ctccagcgaa gcaatcatcg cttccagaga gttaccatgt
      601 tcatgcatcc gccgcagcaa gcggaaacgc tcgctcttcc aggtggtcat cacgataaac
      661 atcacagtac cgaggctcaa tggcaaccag ccgccggaga gcagtttatc gaggttagcg
      721 gtgaacaatg gaatatcgac acaaaggaaa gcaatcagga tcagcgcaac aaaatactta
      781 ttccagtgcc agttctgacg tgccacggta gtcgagagaa tagacgtcag taccatggtt
      841 ccggtcaccg caatcccgta cgccgccgcc aggttgctgg agtgctcaaa gctgacaatc
      901 acaatcacga ccgcgacata gagcatccag ttcacaaacg gaatatagat ttgccctgac
      961 tccatttcgg aggtgtgaat aatgcgcatc ggcgacaaat atcccagacg taccgcctga
     1021 cgcgtcaatg agaagacgcc agagataacc gcctgcgagg caattaccgt cgccagtgcg
     1081 gcgatgatca gcagtgggat cagcgcccag tccggtgcca acaggaagaa cgggttctta
     1141 atcgcttccg ggttctttaa caacaacgct ccctgaccga agtaattaag ggtcaaggaa
     1201 ggtaatacga cggtgaacca cgccaggcga atagggaact taccaaagtg ccccatatca
     1261 gcatacagca cctcgccccc cgtaatcgac agcaccactg cccctaatgc aataaaagaa
     1321 accgttttgt attcaaggaa gaaatgcacc gcccacatcg gattcagcgc atgcagcact
     1381 tccgggttag caataatgct acgtaacccc agtcctgcca gaatcaaaaa ccaggtcagc
     1441 atgatcggcg caaacagctt accgaccata gccgtgccat gcttt
//
LOCUS       DAJTUN010000266         1478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTUN010000266 DAJTUN010000000
VERSION     DAJTUN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.272"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>1478
                     /locus_tag="OO470_004004"
     rRNA            <1..>1478
                     /locus_tag="OO470_004004"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          370 a          337 c          472 g          299 t
ORIGIN      
        1 caagtcgaac ggtaacagga agcagcttgc tgtttcgctg acgagtggcg gacgggtgag
       61 taatgtctgg gaaactgcct gatggagggg gataactact ggaaacggta gctaataccg
      121 cataacgtcg caagaccaaa gagggggacc ttcgggcctc ttgccatcgg atgtgcccag
      181 atgggattag ctagtaggtg gggtaacggc tcacctaggc gacgatccct agctggtctg
      241 agaggatgac cagccacact ggaactgaga cacggtccag actcctacgg gaggcagcag
      301 tggggaatat tgcacaatgg gcgcaagcct gatgcagcca tgccgcgtgt atgaagaagg
      361 ccttcgggtt gtaaagtact ttcagcgggg aggaagggag taaagttaat acctttgctc
      421 attgacgtta cccgcagaag aagcaccggc taactccgtg ccagcagccg cggtaatacg
      481 gagggtgcaa gcgttaatcg gaattactgg gcgtaaagcg cacgcaggcg gtttgttaag
      541 tcagatgtga aatccccggg ctcaacctgg gaactgcatc tgatactggc aagcttgagt
      601 ctcgtagagg ggggtagaat tccaggtgta gcggtgaaat gcgtagagat ctggaggaat
      661 accggtggcg aaggcggccc cctggacgaa gactgacgct caggtgcgaa agcgtgggga
      721 gcaaacagga ttagataccc tggtagtcca cgctgtaaac gatgtcgact tggaggttgt
      781 gcccttgagg tgtggcttcc ggagctaacg cgttaagtcg accgcctggg gagtacggcc
      841 gcaaggttaa aactcaaatg aattgacggg ggcccgcaca agcggtggag catgtggttt
      901 aattcgatgc aacgcgaaga accttacctg gtcttgacat ccacggaagt tttcagagat
      961 gagaatgtgc cttcgggaac cgtgagacag gtgctgcatg gctgtcgtca gctcgtgttg
     1021 tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta tcctttgttg ccagcggtcc
     1081 ggccgggaac tcaaaggaga ctgccagtga taaactggag gaaggtgggg atgacgtcaa
     1141 gtcatcatgg cccttacgac cagggctaca cacgtgctac aatggcgcat acaaagagaa
     1201 gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg tagtccggat tggagtctgc
     1261 aactcgactc catgaagtcg gaatcgctag taatcgtgga tcagaatgcc acggtgaata
     1321 cgttcccggg ccttgtacac accgcccgtc acaccatggg agtgggttgc aaaagaagta
     1381 ggtagcttaa ccttcgggag ggcgcttacc actttgtgat tcatgactgg ggtgaagtcg
     1441 taacaaggta accgtagggg aacctgcggt tggatcac
//
LOCUS       DAJTUN010000267         1464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTUN010000267 DAJTUN010000000
VERSION     DAJTUN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.273"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            84..>1464
                     /gene="glpD"
                     /locus_tag="OO470_004005"
     CDS             84..>1464
                     /gene="glpD"
                     /locus_tag="OO470_004005"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6253062.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWV"
BASE COUNT          345 a          379 c          416 g          324 t
ORIGIN      
        1 caatgttacc caaagcgcga ttctttgcta atatgttcga taacgaacat ttatgagctt
       61 taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata ggtggcggca
      121 tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg
      181 aggcgcagga tctcgcttgc gcgacctctt ccgccagttc aaaactcatt cacggtggcc
      241 tgcgctacct tgagcactat gaattccgcc tggtcagcga ggcgctggct gaacgtgaag
      301 tgctgctgaa aatggctccg catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc
      361 cgcatctgcg cccggcgtgg atgattcgca ttggtctgtt tatgtacgat catctgggta
      421 aacgcaccag cctgccggga tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac
      481 cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc cgtctggtac
      541 tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca
      601 cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc gataccggca
      661 aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt
      721 tcttcgacga ggggatgcat ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc
      781 atattgtggt gccgcgcgtg catacccaaa agcaggccta cattctgcaa aacgaagata
      841 aacgcattgt gttcgtgatc ccgtggatgg acgagttttc catcatcggc accaccgatg
      901 tggaatacaa aggtgatccg aaagcagtga agattgaaga gagtgaaatc aattacctgc
      961 tgaaagtgta taacgcgcac tttaaaaagc agctaagccg tgacgatatc gtctggacct
     1021 actccggtgt gcgtccgctg tgtgatgatg agtccgactc gccgcaagcc attacccgtg
     1081 attacaccct tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg
     1141 gtaagctgac cacctaccga aaactggcag aacatgcgct ggaaaaactg acgccgtatt
     1201 atcagggtat tggcccggcg tggacgaaag agagcgtgct gccgggtggt gccattgaag
     1261 gcgaccgcga cgattacgcc gctcgcctgc gccgccgtta cccgttcctg actgaatcgc
     1321 tggcacgtca ctacacgcgc acttacggca gcaacagcga gctgctgctc ggcaatgcgg
     1381 gaacggtaag cgatctcggg gaagatttcg gtcatgagtt ctacgaagcg gaactgaaat
     1441 acctggtgga tcacgaatgg gtcc
//
LOCUS       DAJTUN010000268         1346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTUN010000268 DAJTUN010000000
VERSION     DAJTUN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.274"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..371
                     /locus_tag="OO470_004006"
     CDS             <1..371
                     /locus_tag="OO470_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253063.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGTWYNVASI"
     gene            374..919
                     /locus_tag="OO470_004007"
     CDS             374..919
                     /locus_tag="OO470_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6253064.1"
                     /translation="MLHLKSITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITTNRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            943..>1346
                     /locus_tag="OO470_004008"
     CDS             943..>1346
                     /locus_tag="OO470_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6253065.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAA"
BASE COUNT          373 a          298 c          371 g          304 t
ORIGIN      
        1 atccatccag gcctgttgtt gcagagcttg gcgatgattc aggctggcat tttttcagcc
       61 agagaaaccc ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt
      121 acggaaactt cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg
      181 gccatgaaat gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt
      241 cgggacacgg gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg
      301 ttgacggtgt gtattaccga ccactgcaaa aactgattaa tggcacctgg tataacgtag
      361 cgagtattta acaatgttgc atttaaaaag tattactgcg ggtaatccga aaacagcaga
      421 acaatatcag atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa
      481 ctggtatgaa gagctgaaga atttcgccag cgacacaata aaaatagctt acaccggaga
      541 tggtcgcgtg gtgtgggtcg gtaaggatgt gacaggcatc gagccacgca atgccagcgt
      601 tatcgaagtt ccggatatta ccactaaccg acggattacc gcgccgggtt actggtttta
      661 ccgcaatgat gaatttgtct ttgactacag actcaaagcg gaagatgagc gtgatgccct
      721 tctggctcag gtcagtgccc ggacagggga atgggaagaa gacctgctgc tggggttaat
      781 cagcgacgaa gataaagaaa agctgaaagc ctgtcgtatt tacgcgaaat cgctgcaggc
      841 gatggatttc agcaccatca ctgataaatc ctcatacaac gccattgaat ggcccgcctc
      901 tccggaaggt tcttcctgat ttaatttatc gcgagaaaaa caatgtctgt agtgatatca
      961 ggtgcgctga ctgatggcgc aggtatcccc atgtccggat accatattat tctgaaatcc
     1021 cgggtaaaca ccccggaagt ggtgatgaac actgttgctg atgtgatgac aggaaacgat
     1081 ggtgaatact gtttccatgc gcggactgga aaatatggtg tgtatctgaa acaggactgg
     1141 cgcaacgagt acaacgttgg cgacattgct gtatatgagg actcaaagcc cggcacgctg
     1201 aatgactttc tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt
     1261 gaggaaatgg tggcgcaggc gcagcagagc gccggggccg cagccggaaa cgcacagcag
     1321 acggcgcagg atgtggcggc agccgc
//
LOCUS       DAJTUN010000269         1330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTUN010000269 DAJTUN010000000
VERSION     DAJTUN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.275"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            14..>1330
                     /gene="maeA"
                     /locus_tag="OO470_004009"
     CDS             14..>1330
                     /gene="maeA"
                     /locus_tag="OO470_004009"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6253066.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNAL"
BASE COUNT          344 a          334 c          353 g          299 t
ORIGIN      
        1 aagagtgagt gacatggaac caaaaacaaa aaaacagcgt tcgctttata tcccttacgc
       61 tggccctgta ctgctggaat ttccgttgtt gaataaaggc agtgccttca gcatggaaga
      121 acgccgtaac ttcaacctgc tggggttact gccggaagtg gtcgaaacca tcgaagaaca
      181 agcggaacga gcatggatcc agtatcaggg attcaaaacc gaaatcgaca aacacatcta
      241 cctgcgtaac atccaggaca ccaacgaaac cctcttctac cgtctggtaa acaatcatct
      301 tgatgagatg atgcctgtta tttatacccc aaccgtcggc gcagcctgtg agcgtttttc
      361 tgagatctac cgccgttcac gcggcgtgtt tatctcttac cagaaccgcc acaatatgga
      421 cgatattctg caaaacgtgc cgaaccataa tattaaagtg attgtggtga ctgacggtga
      481 acgtattctg gggcttggtg accagggcat cggcgggatg ggcattccga tcggtaaact
      541 gtcgctctat accgcctgtg gcggcatcag cccggcgtat acccttccgg tggtgctgga
      601 tgtcggaacg aacaaccaac agcggcttaa cgatccgctg tatatgggct ggcgtaatcc
      661 gcgtatcact gacgatgaat actatgaatt cgttgatgaa tttatccagg ctgtgaaaca
      721 acgctggccg gacgtgctgt tgcagtttga agactttgct caaaaaaatg cgatgccgtt
      781 acttaaccgc tatcgcaatg aaatttgttc ttttaacgat gacattcagg gcactgcggc
      841 ggtaacagtc ggcacactga tcgcagccag ccgcgcggca ggtggtcagt taagcgagaa
      901 aaaaatcgtc ttccttggcg caggttcagc gggatgcggc attgccgaaa tgatcatcgc
      961 ccagacccag cgcgaaggat taagcgagga agcagcgcgg cagaaagtct ttatggtcga
     1021 tcgctttggc ctgctgacgg acaagatgcc gaacctgctg cctttccaga ccaaactggt
     1081 gcagaagcgc gaaaacctca gtgactggga taccgacagc gatgtgttgt cactgctgga
     1141 tgtggtgcgc aatgtaaaac cagatattct gattggcgtc tcaggacaga ccgggctgtt
     1201 tacggaagag atcatccgtg agatgcataa acactgtccg cgtccgatcg tgatgccgct
     1261 gtctaacccg acgtctcgcg tggaagccac accgcaggac attatcgcct ggaccgaagg
     1321 taacgcgctg
//
LOCUS       DAJTUN010000270         1306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTUN010000270 DAJTUN010000000
VERSION     DAJTUN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.276"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..182
                     /locus_tag="OO470_004010"
     CDS             <1..182
                     /locus_tag="OO470_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253067.1"
                     /translation="VVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEI
                     PVLDVSDISVKLQEA"
     gene            184..789
                     /locus_tag="OO470_004011"
     CDS             184..789
                     /locus_tag="OO470_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6253068.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            776..>1306
                     /locus_tag="OO470_004012"
     CDS             776..>1306
                     /locus_tag="OO470_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6253069.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHRE"
BASE COUNT          313 a          306 c          361 g          326 t
ORIGIN      
        1 ctgttgtctc tgccgggcgc tactatgccg gcatttacaa aaccgatccg gaacatattg
       61 atattctggg ccttactgtg agccgtgatg gttcgtcatg gacaacggct gtcactttcg
      121 ggatagatga gattccggtt ctggatgtgt cggatatcag tgtgaaactc caggaggcat
      181 aacgtgcaga atgtggctgc cactgtgctt gcgcagtatg ctgccagccc ccgactcaat
      241 gccctcatta acagctttaa cgcagcgctt tcccccgaca gttttatcag tgatttttat
      301 ggtcttatct ggaacatcga taccgcagaa aagtatggtc ttgatgtctg gggaaagatt
      361 gtgggtgtca gttgctggct gacggtaaag gacgatttta attacctggg cttcagcgag
      421 tccaggatgg acaccccggt aatggatgat ccctgtccgt ttaatcaggc accgttttac
      481 aacggaaaat cggatacccg gactgttgac ctgtctgatg ctgtataccg gcggctgata
      541 ctgatgaaag ccatgtcgaa cattactgac tgttccgttc cggatattaa cctgatgctg
      601 agatttatgt tcggaaaaaa acgccgggct tatgttctga ataatggcgg gctgaggatg
      661 agttacgtct ttgagtccgc gctctcgttg gcagaactgg cgattatcca gtcgtcgggt
      721 gcactgccat ccccgccggg tgtttatgtt tcagtagttt taaaggagtc ccgtaatgaa
      781 ggccagtgat aaaccccgcc agctggcggt cccctttgcg agtaccggag ataaaaaccg
      841 tatcccggac aaggcgacac agcagaccag agagagcggt aatgctgcgt atgattcagg
      901 ttttcctccg gtgaccatga cagcggtctc agcgggaggt ataccgccac acggcaagga
      961 ttttaacggt ctgatgtacg atattaccgc agcaatacgg ttcgcccagg ctggcggttt
     1021 gtacacgtat aatgccggtt ttgcgggggc cattggtgga tatgcaaaag gagccattct
     1081 cgccggagtc gcaacaacag cggtctggct gaataccacg gacgataacc tgaccgatcc
     1141 tgaaggctcc gacagtgcgg gctgggtaaa tcttcttgag gatccgaaaa ggatattcct
     1201 gcggcagaag aacaatctgt cagaccttca gaataaaggg acggcacggg ataatcttca
     1261 ggtttacagt aaagagcagt cagatcaacg ctatgttcat cgggaa
//
LOCUS       DAJTUN010000271         1292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTUN010000271 DAJTUN010000000
VERSION     DAJTUN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.277"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..576
                     /locus_tag="OO470_004013"
     CDS             <1..576
                     /locus_tag="OO470_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HCR6253070.1"
                     /translation="TDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPK
                     QFAMSGAFSGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAIN
                     WLRYMEISRSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSL
                     RFGDNLQAWLEQNARLPGNDCPQGKECEEKQ"
     gene            573..758
                     /locus_tag="OO470_004014"
     CDS             573..758
                     /locus_tag="OO470_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6253071.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            763..1092
                     /locus_tag="OO470_004015"
     CDS             763..1092
                     /locus_tag="OO470_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6253072.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
BASE COUNT          391 a          250 c          329 g          322 t
ORIGIN      
        1 actgatgtta gcgtcgatgt gttaggcggt aacgtgctga tgaaacaatt acgtatgccg
       61 caacatgacc cggcgctgtt gcggctgaat aatctctcat ccagcgaact ggttagcgcc
      121 gtcaatccga aacaattcgc catgtccggg gcatttagtg gtgcattgcc gttatggcta
      181 aacaacgaaa aatggatagt gaaagatggt tggctggcaa atagcgggcc aatgaccttg
      241 cggctggata aagataccgc agatgcggtg gtgaaagaca atatgactgc gggttcagca
      301 attaactggt tgcgctatat ggaaattagc cggtcatcga caaaaattaa cttagataat
      361 ctcggtttat taaccatgca ggccaacatt acaggtacca gtcgcgttga tggtaaaagc
      421 ggtacggtaa accttaatta ccatcatgaa gagaatattt ttacgctgtg gcgcagttta
      481 cgctttggcg ataatctcca ggcatggttg gagcagaacg cacgtctgcc gggaaatgac
      541 tgtccgcaag gaaaagagtg tgaggaaaaa caatgaaaat tttactggct gcgttgacgt
      601 catcttttat gctggttggc tgtacgcctc gcattgaagt cgctgcacct aaggaaccga
      661 tcactatcaa tatgaacgtt aaaattgagc atgagatcat catcaaggca gacaaagatg
      721 tcgaagagct gcttgaaact cgtagcgatc ttttctgagg cgatgatgaa gaaaacatta
      781 cttctttgtg cgtttcttgt tgggctggta agcagcaatg taatggcatt gactctggat
      841 gaagccagaa ctcaggggcg ggtaggtgaa acattttatg gttatctggt tgcgctgaaa
      901 acggatgctg aaacagagaa attagtaacc gaaattaatg ccgaacgtaa agcgagttac
      961 caacaacggg caaagcaaaa taatgtgtct gtagatgata tcgcgaaact cgccgggcaa
     1021 aagctcgtag ctcgggccaa accgggggaa tatgtacaag ggataaacgg taaatgggtg
     1081 cgaaagtttt aatagcaaaa ccgcaatatc gggataacgc tacaggagaa agcgattgat
     1141 ctattttcct gaaacaaggt gaatattcaa aaactcctgt caaattgcct ttcgccctga
     1201 aaaatgcata gtcaaatatc tgttttaact aattggcgtt gcagtacatg caacgccaat
     1261 tagttagcgg aatttacgtc gatactcgcc tg
//
LOCUS       DAJTUN010000272         1263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTUN010000272 DAJTUN010000000
VERSION     DAJTUN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.278"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            30..449
                     /gene="rbsD"
                     /locus_tag="OO470_004016"
     CDS             30..449
                     /gene="rbsD"
                     /locus_tag="OO470_004016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6253073.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            457..813
                     /locus_tag="OO470_004017"
     CDS             457..813
                     /locus_tag="OO470_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6253074.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTR"
     gene            864..1263
                     /locus_tag="OO470_004018"
                     /pseudo
     CDS             864..1263
                     /locus_tag="OO470_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          329 a          311 c          319 g          304 t
ORIGIN      
        1 agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt aattctgata
       61 tttcatcggt gatctcccgt ctgggacata ccgatacgct ggtggtgtgt gatgctggtt
      121 tacccatccc caaaagtaca acgcgtatcg atatggcatt aacccaaggt gtaccttctt
      181 ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgcc attatcgcgg
      241 aagagatcaa acaacataat ccgcaactcc acgaaacgtt gctcactcac cttgagcagc
      301 tgcaaaaaca ccagggaaat accattgaaa ttcgttacac cacgcatgaa caattcaaac
      361 aacaaaccgc agaaagtcag gcggtaattc gcagcggaga atgttctccg tatgcgaata
      421 tcattctctg tgctggcgtg acgttctgag gccgtcatgg aagcattact tcagcttaaa
      481 ggcatcgata aagccttccc gggcgtaaaa gccctctcgg gcgcagcgtt aaatgtctat
      541 ccgggccgtg tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac catgatgaaa
      601 gtgcttactg gtatctatac tcgcgatgcc ggcacgcttt tatggctggg gaaagaaacg
      661 acatttaccg ggccgaaatc ttcccaggaa gccgggattg ggattatcca tcaggaactg
      721 aacctgatcc cgcagttgac cattgccgaa aacattttcc tcggtcgtga gtttgttaat
      781 cgctttggca aaattgactg gaaaaccagg taatgctgcc aacttactga tttagtgtat
      841 gatggtgatt ttaaggtgct tgcgtggctt ccatttccat cagatgtcct tcctgctccg
      901 ctactgaagg cgtggtgcgt aacggcaaaa gcactgccgg acatcagcgc tatctctgct
      961 ctcattgccg taaaacatgg caactacagt tcacttacac cgcctctcag cccggtacgc
     1021 accagaaaat cattgatatg gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta
     1081 tgggcgttgg cctcaacacg gttttacgtc acttaaaaaa ctcaggccgc agtcggtaac
     1141 ctcgcgcata caaccgggca gtgatgtgat tgtctgcgct gaaatggacg aacattgggg
     1201 ctacgtcggt gctaaatcac gtcagcgctg gctgttttac gcgtatgaca ggatacggag
     1261 gac
//
LOCUS       DAJTUN010000273         1230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTUN010000273 DAJTUN010000000
VERSION     DAJTUN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.279"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>1230
                     /locus_tag="OO470_004019"
     CDS             <1..>1230
                     /locus_tag="OO470_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6253075.1"
                     /translation="VAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEVVKQKGRVTGS
                     EYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTFVIDGGTRFVV
                     GWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVVGILPRLGIKH
                     PTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRDLRSAFNALQQ
                     DKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRHYSPTEFRKKR
                     QAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDEHGRKVLISYD
                     IHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRKRAEEKIRDAE
                     DEGIQILEHKQAEPWLSN"
BASE COUNT          320 a          295 c          357 g          258 t
ORIGIN      
        1 gtcgcagaat ggcagcgtcg ccatgcggac gaaccgtaca tgctggaggt gatgccttcc
       61 tatgatgttg tccggtatgc catgaaaaaa ctgccggaag tggtgaaaca aaaagggcgc
      121 gttaccggca gtgaataccg tcagcttgaa ggattcaccc gccgcgactg gacagctatg
      181 ccggtgaatt atgtctggat tggtgacggt cacggtatga agctgaaatg tgcgcatccg
      241 atccacgggc gtccattctc accggaagtc acgtttgtga tcgatggtgg aacacggttt
      301 gttgttggct ggagtcttga ccttgccgaa aacgtgttcg ccgttgccgg tgcgatacag
      361 cacggcattc gcaaccacgg aaaaccgttc ctgtattact cggataacgg ttccggtgaa
      421 acggctgaca tgctggacaa agaggtcgtg ggtattctgc cacgcctggg gattaaacac
      481 cctaccggta ttgccggtaa tccacaggga cgcggcatta ttgaacgcct gaaccggaca
      541 ctgccgatgc gtatagccag gaggtatcgc acctattttg gcaagggtgc tgaccgggaa
      601 tcgttgcgtg tacttaaccg tgatctgcgt tcagcgttta acgccctgca acaggacaag
      661 cctctgaacg accgccagaa agcggcaatg cgcgagctgc catcgtgggc ggagcttatt
      721 gaggctattc gtgaaggcgt ggaatggtac aacaaccgcc cgcattctga gctgccgatg
      781 aaaccgaacg gacggcatta cagcccgaca gagttcagaa aaaaacgcca ggcagaagaa
      841 gacaccgaaa ttgagtggct gagtgatctt gagctgcgcg acatgttccg cccaatggtg
      901 gaacgaccgg taagacgctg cgaaattcag tggctgaaca atatttacta cgcgccggag
      961 ttgcgtgacg aacacggtcg caaagtgctt atcagttatg acattcatga cgccgagcga
     1021 attacggtac gtcgcaagga cggcagcttt atctgtgagg cgatatggaa cggcaataaa
     1081 cgcgcagcct ttgctgtcag tgctgaatat cacaaacaac agcagcgtat taaaggaatg
     1141 cgcaaacgcg ctgaggaaaa aattcgtgat gctgaagatg aaggtattca gattctggaa
     1201 cacaaacagg ctgaaccctg gctgagtaat
//
LOCUS       DAJTUN010000274         1224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTUN010000274 DAJTUN010000000
VERSION     DAJTUN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.280"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..477)
                     /gene="nhaR"
                     /locus_tag="OO470_004020"
     CDS             complement(<1..477)
                     /gene="nhaR"
                     /locus_tag="OO470_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6253076.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEG"
     gene            complement(543..>1224)
                     /gene="nhaA"
                     /locus_tag="OO470_004021"
     CDS             complement(543..>1224)
                     /gene="nhaA"
                     /locus_tag="OO470_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6253077.1"
                     /translation="DDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTG
                     VYILVGVVLWTAVLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLIL
                     PLFAFANAGVSLQGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEG
                     TTYQQIMVVGILCGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWL
                     RVRLRPSV"
BASE COUNT          348 a          338 c          277 g          261 t
ORIGIN      
        1 gccttcctgc tgcgtagagt ctatcggaca gtcagaaatg atcatatcca gtttatgctg
       61 acttaattgc tccagcagca tttcgtgggt ggattcgaag cagcgaagat gaatgggctc
      121 gccttctacc actgcggcgt taagtacgct actgaccagg cgtttggaaa gtgcatcagc
      181 cacgccaacg tcaaacaata aattggattc tttgcgatag ttcacaatat ccagcatttc
      241 ctggcttaag gtgaacattt tatcggcata gcgatagacc agttctccca gctcgctggg
      301 ttcgagacca cgtcccttgc gtttaaataa tttgccttgc aggcgctctt ccagcgctcg
      361 aatctgtccg gtaatggttt gtggagttaa ataaagcgcc tccgctgcgc caaccacgga
      421 accttcttta tagacatgcc agaagtaata caagtggttg taattgatat gagacatgct
      481 catttctctc cctgataaca atgaaaaggg agccgtttat ggctccccgg taaaccgtcc
      541 tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt atccaattac cgccgaagag
      601 atagaaccga ccaggatacc gagtttcgcc cagttaatca gttctggatc tacgctacca
      661 aaggccaggc tggcaataaa gatagacata gtaaaaccga taccgcacag gatccccacc
      721 accataattt gctgataagt cgttccctca ggcagatgcg ccagtttcaa acgcagcgcc
      781 aaccagcaga acagactaat ccccagcggt ttgccaatca gcaagccagc gatgatcccc
      841 aatggcagaa tggaggtcaa gccatccagc gtgacgcctt gcagtgaaac gccagcatta
      901 gcaaatgcaa acagcggcaa aatcagatac gccacccacg ggtgcaacac atgctccagt
      961 cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa taaagaagcc gacaattacc
     1021 cccgccagag ttgcgtgaac ccccgatttc aacaccgcag tccacaacac cacgccaaca
     1081 agaatataga cgcccgtgcg gcgtacacca cacagattca ataccgcgag taccgcaatt
     1141 gctacagccg cgacgccaag agaggccatc gataagtcat tagtgtagaa caatgcgatg
     1201 ataatgatgg ccccaagatc gtcg
//
LOCUS       DAJTUN010000275         1205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTUN010000275 DAJTUN010000000
VERSION     DAJTUN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.281"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..75)
                     /locus_tag="OO470_004022"
     CDS             complement(<1..75)
                     /locus_tag="OO470_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6253078.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALF"
     gene            complement(141..380)
                     /locus_tag="OO470_004023"
     CDS             complement(141..380)
                     /locus_tag="OO470_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6253079.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(515..1120)
                     /locus_tag="OO470_004024"
     CDS             complement(515..1120)
                     /locus_tag="OO470_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6253080.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          349 a          274 c          204 g          378 t
ORIGIN      
        1 aaagagcgca acaaataaaa gatcgactct tagtaaccat attgctgcca ttgaaagact
       61 ccatgtacca tacaaggttg ctgccagaca tattccttta cgtctgacag cctctagaga
      121 gtttttttat tttttataag ttacggatta ctcgttccgg aaatcgttac ctgttgaatt
      181 gctgcagcga tagcgtcgaa tgcatcttta attgcgccca ggaagcctga gttctgatcg
      241 ccaaagatga aagcaagcag agttgccata gcaacaccaa tcaatgcata ttcgattgca
      301 gtaacaccac gttcatcctt cgcgaaacgc gcaaagcact gtttagtttt accatagtac
      361 ttagtaataa aagtattcat gttttcctcg tatattatta tttaaattca aaccaccagc
      421 ttcatctgcg tgtcaccggt atcccatatt ttttggatgt ggctataatt atccattccc
      481 gctatttagt cttcaaaaaa aattacattt aatatcattt atcttgatta agaaatagta
      541 agactaaaaa aacaagcgtc gtcagccagc acactgctaa cacggtaaga acgctaataa
      601 aaacactcca ctgcttcgtt ctataccatt caatattttt gtcagtatgt tttattggga
      661 gcagctcttc agtagagaat tcctggctgt tatcattctc aggaatgttt gcatcagtgt
      721 ttacttccct ggcctcgcta tcgttggtat agctactact gtcttcatca ctgtcacgct
      781 cattctcaca gacatgtttc tcgacctttc ccagatatct gacgcctttt cgcggaatgg
      841 ttttaattag agaactcgac aaacctgcga cgtctaatgc ctttctaagc atgtaaatct
      901 gcccataata actgctgtcg cttaccgaac ctttctgctc ccgccaaacc tcattgataa
      961 tatgttgctt atcggattct ccctgtagta aaaggagtaa aaatcgatag ccgttttcag
     1021 acagattgat tacattgcct gtactggtgg tgatacgtcg agacgctggg taaaacttaa
     1081 ctccctgccc taaagaaaaa taaaaacggt catcgctcat aagtaccgac ccctgttttc
     1141 ttcttcacat gactactcat tacaactttg tatgaagtaa gatgcaaaat catattgttt
     1201 acatt
//
LOCUS       DAJTUN010000276         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTUN010000276 DAJTUN010000000
VERSION     DAJTUN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.282"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..114
                     /locus_tag="OO470_004025"
     CDS             <1..114
                     /locus_tag="OO470_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6253081.1"
                     /translation="NSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            126..>1190
                     /gene="proA"
                     /locus_tag="OO470_004026"
     CDS             126..>1190
                     /gene="proA"
                     /locus_tag="OO470_004026"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6253082.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRF"
BASE COUNT          298 a          299 c          344 g          249 t
ORIGIN      
        1 aacagcgatg cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg
       61 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagct
      121 ggccgatgct ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac
      181 tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa
      241 gcgaaatcat cctcaacgct aacgcccagg atgttgctga cgcgcgagcc aatggcctta
      301 gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgacg
      361 atgtacgtca ggtgtgcaac ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac
      421 tggacagcgg cctgcgtctt gagcgtcgtc gcgtaccgct gggggttatt ggcgtgattt
      481 atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg
      541 cggtgatcct gcgtggtggt aaagaaacct gtcgcactaa cgcggcaacg gtggcggtga
      601 ttcaggacgc cctgaaatcc tgtggcttac cggcgggtgc cgtgcaggcg attgataatc
      661 ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc
      721 cgcgtggtgg cgctggtttg cataaactgt gccgtgaaca gtcgacaatc ccggtgatca
      781 caggtggtat aggcgtatgc catatttacg ttgatgaaag tgcagagatc gctgaagcat
      841 taaaagtgat cgtcaacgcg aaaactcagc gtccgagcac atgtaatacg gttgaaacgt
      901 tgctggtgaa taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg
      961 aaagcggcgt gacattacac gcagatgcag ctgcgctgac acagttgcag gcaggccctg
     1021 cgaaggtggt ggcggttaaa gccgaagagt atgacgatga gtttctgtca ttagatttga
     1081 acgtcaaaat cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac
     1141 aacactccga tgcgatcctg acccgcgata tgcgcaacgc ccagcgtttt
//
LOCUS       DAJTUN010000277         1187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTUN010000277 DAJTUN010000000
VERSION     DAJTUN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.283"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..923)
                     /locus_tag="OO470_004027"
     CDS             complement(<1..923)
                     /locus_tag="OO470_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6253083.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLG"
     gene            complement(1070..1146)
                     /locus_tag="OO470_004028"
     tRNA            complement(1070..1146)
                     /locus_tag="OO470_004028"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1110..1112),aa:Pro,seq:tgg)
BASE COUNT          303 a          320 c          288 g          276 t
ORIGIN      
        1 cccagccgat attgcggata aacatagtga tcgcaggcgt aaacgtcgcc gttgtgctca
       61 acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcacccgg cgcaccgaca
      121 aaattggcaa acgcccattc gatattcatc acgaaaatct tgccgacgtc gcgtttgatc
      181 cagtggtcaa aaatcgccac cagaaattca ccaaattcat cggggcgcac agaccattcc
      241 gtcagttccc cctgaatatc accaggcgca tgtagtttca gtccatcgcg gacagctgtt
      301 tcatcagcca ggcgctcgac caccggaata aactggatga attcgactcc cgcatcgcac
      361 aaaaaatcat aaacctgtaa cggttgctgc gcgctggtgc ggttgacgca gaccagcacg
      421 ttatagtcga catgatgttt ttgcaggagc gtcagggcac gcatcaccag tttatgcgtg
      481 ggtctgccgc ctttggtcac gcgatattga ttgtggatct cagccggacc atccagcgat
      541 aacccaacaa gaaaattatt ttctgccaga aatgcacacc attcgtcatc gagcagcacg
      601 ccgttagtct ggaaactgtt acttatcttc ctgccagcac catatttcgc ctgtagcgcc
      661 acggcacggc ggtaaaactc cagcccgagt agcgttggtt cgccgccctg ccaggtaaaa
      721 gccacttcgt tttgcagttc gctggcggcg atatagtgac ggatatacgc ttccagcgtg
      781 tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc gagataaaaa
      841 cagtagtcac agttcagatt acaatcggaa ccgctcggtt tcgctatcac atgaaaagca
      901 cgcgttggaa cctgttgcag catggtcgct ccttgcctga agtcatcctt caatacgtta
      961 agggcgaaat cataccgtgg ggcaatgagt tattacgggc gtttattgcg aagggagggt
     1021 gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt ggtcggcgag
     1081 agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa gctgcgctac
     1141 tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtc
//
LOCUS       DAJTUN010000278         1110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTUN010000278 DAJTUN010000000
VERSION     DAJTUN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.284"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(1..508)
                     /locus_tag="OO470_004029"
                     /pseudo
     CDS             complement(1..508)
                     /locus_tag="OO470_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(556..1095)
                     /locus_tag="OO470_004030"
     CDS             complement(556..1095)
                     /locus_tag="OO470_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015570147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6253084.1"
                     /translation="MASLMLAGHALAEDGVVNFVGKIVDTTCEVTSDTADQIVPLGKV
                     SKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGTPLTAGNP
                     GDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIARYVATNA
                     TVTAGTANADSQFTVEYKK"
BASE COUNT          301 a          256 c          236 g          317 t
ORIGIN      
        1 tcactttcgg cgcagcatct tcgggaataa ggcggatatt tgctggtcgc cagaacagct
       61 tgatacgcgt ttttactgct atttccagcg agttcgtcgc tttgttgctc gctgatggcg
      121 gaatattttt aatattcagc cagaagactg actcgcgatc atcaggcaat gacacacctt
      181 tggtgtgaat aatgcgtaat attttttctt gcgaaggttc taatcgtgat actggcggaa
      241 taacggtaaa cggcgctttg cttttatcct ctttattaaa tggatctacc caagcctgga
      301 tgagataagg tattttttct tctttgctaa atacagaaat agatttatca gcattattac
      361 ttaagtaaac cacccgcgta ctaccaatga caacgcctgc ataagttaat gctggttggc
      421 ctaaaaaaac gctgagtata atcgcggtaa ataacttaca ttaaactgtt gttttaaaga
      481 gcctgattaa acaacttttt ctggacataa cgactccctg gccaaagcca actgaataaa
      541 aaggcccttg ataaattatt tcttatattc gacagtaaat tgtgaatcag cgttcgccgt
      601 accagccgtt acggtcgcat tggttgccac atagcgtgca ataaacttca tttcagcctt
      661 gccttcagca tcgatagcgg tataagcaga gtcgttataa agtttaacct gcgaattatc
      721 ggactggtta aaaatacgaa taccaacgcc ggttgccgca atcgcttgtc ctgtaccggt
      781 aaaatcacca gggttgcctg ctgtaagcgg cgtacccact ttcaggtcgc cgtcgccgcc
      841 aggcgcttct gtaccatcaa aacgaacggc tgcttgagtg tacgttgccg ggcaattttc
      901 aagtttaata ctgaacttct gtggcgacgc cagactacct acacctgaaa atgcattttt
      961 actgacttta cccagtggga caatttgatc ggctgtatcg gaggtaactt cacaagtagt
     1021 gtcgacaatt ttaccgacga agttaacaac accatcttct gccagtgcat gcccggccag
     1081 cattagagat gccagagtga atgcaccgcc
//
LOCUS       DAJTUN010000279         1101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTUN010000279 DAJTUN010000000
VERSION     DAJTUN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.285"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>1101
                     /locus_tag="OO470_004031"
     CDS             join(<1..199,199..>1101)
                     /locus_tag="OO470_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCR6253085.1"
                     /translation="VVDQKYTVADAAKAMDVGLSTMTRWVKQLRDERQGKTPKASPIT
                     PEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSIIGKLRAHYPVVTLCHVFG
                     VHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWL
                     AGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGK
                     RWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYT
                     SRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITD
                     YIVGYYSALRPHEYNGGLPPNESENRYWKNSNS"
BASE COUNT          301 a          271 c          310 g          219 t
ORIGIN      
        1 gttgttgacc agaaatacac ggtggcagat gccgccaaag ctatggatgt tggcctttcc
       61 acaatgacaa gatgggtcaa acaactgcgt gatgagcgtc agggcaaaac accaaaagcc
      121 tcccccatta ccccggaaca aattgaaatc cgtgagctca ggaaaaagct acaacgcatt
      181 gaaatggaga atgaaatatt aaaaaaggct accgcgctct tgatgtcaga ctccctgaac
      241 agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt
      301 tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca
      361 gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac ggttcggccg
      421 gagcaagaag catcgccaca atggcaaccc ggagaggcta ccagatggga cgctggcttg
      481 ctggcaggct catgaaagag ctggggctgg tcagctgtca gcagccgact caccggtata
      541 aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaaaggcag ttcgccgtga
      601 ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg
      661 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
      721 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
      781 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
      841 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
      901 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
      961 gttacgtaag cttcagcgag gcagctcacg ccataacgga ctatatcgtt ggatattaca
     1021 gtgcactaag accgcacgaa tataacggtg ggttaccccc aaacgaatcg gaaaatcgat
     1081 actggaaaaa ctctaactcg g
//
LOCUS       DAJTUN010000280         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTUN010000280 DAJTUN010000000
VERSION     DAJTUN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.286"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(72..992)
                     /gene="speB"
                     /locus_tag="OO470_004032"
     CDS             complement(72..992)
                     /gene="speB"
                     /locus_tag="OO470_004032"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6253086.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT          263 a          317 c          285 g          235 t
ORIGIN      
        1 ggtcggataa ggcgttcacg ccgcatccga cattaatggc acgttttacc cgtgcgcatc
       61 gcatctggtg cttactcgcc ctttttcgcc gcctgaatat acagcatctc cagcgccagc
      121 gtcgccgcag ccagagcggt gatttccgac tgatcgtatg ccggagccac ttccactacg
      181 tccatcccaa cgatgttgag atctttcagg ccgcgtacca gtttaatggc gcgatcggag
      241 gtcaggccgc caatcactgg cgtaccggtt cccggcgcga aagcaggatc caggcagtcg
      301 atatcaaaag tcaggtaaac cggcatatca cccacaatct gtttcacctg ggcgataacg
      361 tcatccacgc tgcgatcgtt cacctggcag gcgtccagca cggtaaagcc gttgtcttta
      421 tcaaactcgg tacgaatacc aatctgcacg gaatgattcg ggtcgatcag accttctttc
      481 ggcgcggtat agaacatagt gccgtggtca aattcacaac cgttcgcata ggtatcggtg
      541 tgggcgtcaa agtgtaccag cgccattttg ccgaaatgct tcgcatgagc acgcagcagc
      601 ggcagcgtaa caaagtggtc accaccgaag gagagcatac gcttaccggc agccagcagc
      661 ttctcggcgt gcgcctgcag cttttcgctc atctcacggg catcgccaaa ggcatatacc
      721 agatcgccgc agtccacgac gttcagacgc tcacgcatgt cgaaattcca cgggaagcgg
      781 ttgtgttccc aggccagatt cgtcgaaacc tgacggatcg ctgccggacc gtggcgacca
      841 cccgcacgac cagaagtggc catatcgaac ggcacgccag taatcaccca gtctgcatcg
      901 ctgtcatacg gctggaagtt catcggcagg cgtaaaaaac caaaggcatt ggaaaccagt
      961 gagttatcgt attgatgacc taaggtgctc atggatggac tcctattaca aagtcgatat
     1021 aaaaaaaccc ttccgcgtcg ttaggcccga cgaggaaggg ttggatttgc gaaacacaag
     1081 tttgtgctaa ttatcgcctt
//
LOCUS       DAJTUN010000281         1080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTUN010000281 DAJTUN010000000
VERSION     DAJTUN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.287"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..761
                     /locus_tag="OO470_004033"
     CDS             <1..761
                     /locus_tag="OO470_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HCR6253087.1"
                     /translation="NPGTAAGTLPGEATVDPAFGLNAIWIESALKEQAQIQGYTVVEA
                     STVVATHLNHLISQHAAELFGRQEAQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQ
                     NLLDEKVPIRDMRTILETLAEHAPIQSDPHELTAVVRVALGRAITQQWFPGKDEVHVI
                     GLDTPLERLLLQALQGGGGLEPGLADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLL
                     SRFLRRSLPQLVVLSNLELSDNRHIRMTATIGGK"
     gene            761..>1080
                     /gene="flhe"
                     /locus_tag="OO470_004034"
     CDS             761..>1080
                     /gene="flhe"
                     /locus_tag="OO470_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6253088.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIW"
BASE COUNT          230 a          279 c          325 g          246 t
ORIGIN      
        1 ttaaccctgg aaccgctgcc gggacgttac ctggtgaggc gaccgtcgat ccggcatttg
       61 gcctgaatgc tatctggatt gaaagtgcgc taaaagaaca ggcgcagatt caggggtaca
      121 cagtggttga ggccagcacg gtggtagcaa cgcatcttaa ccacctcatt agccagcatg
      181 ccgcagagct gtttggtcgt caggaggcgc aacagctgtt ggatcgcgtc gcccaggaga
      241 tgccaaagct gacggaagat ctcgttcctg gcgtcgtcac gctcaccaca ctgcataaag
      301 tgctgcaaaa tctcctcgat gaaaaagtac cgattcgcga tatgcgcacc attctcgaaa
      361 cgctggcgga acatgcgccc atccaaagcg atccacatga attaaccgcc gtcgtgcgcg
      421 tggcgttggg acgggcgatt acccagcagt ggtttcctgg caaagatgaa gtccatgtta
      481 ttggcctcga tacaccgctg gaacgtttgt tactacaggc gctgcagggc gggggaggac
      541 tggagccagg gctggcggat cgtttactgg cgcaaactca ggaagctcta tcccgtcagg
      601 agatgctggg tgcgccgcca gtattgttgg tgaaccacgc gctgcgacca ttattgtctc
      661 gcttcctgcg ccgcagcttg ccgcagttag tggtcctgtc gaatctggaa ctgtctgata
      721 accgacatat ccgcatgacg gcgacaattg gcggtaaatg atgagaacct tattagcgat
      781 attattgttt ccgctgctgg tgcaagccgc cggggagggg atgtggcagg caagtagtgt
      841 cggaattacg ctaaatcatc gcggtgagtc gatgtcgtct gcacctcttt ctgcgcgaca
      901 acctgcttca ggattgatga cgctggtggc gtggcgttat cagcttatcg gcccgacacc
      961 tgcaggactg cgggttcgct tgtgttcgca atctcgttgt gtcgaattag aggggcagag
     1021 cggtaccacc gtggcctttt ccggtatagc ggcagcagaa ccgttgcgat ttatctggga
//
LOCUS       DAJTUN010000282         1068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTUN010000282 DAJTUN010000000
VERSION     DAJTUN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.288"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..399)
                     /gene="yjdN"
                     /locus_tag="OO470_004035"
     CDS             complement(<1..399)
                     /gene="yjdN"
                     /locus_tag="OO470_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6253089.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFG"
     gene            complement(557..892)
                     /locus_tag="OO470_004036"
     CDS             complement(557..892)
                     /locus_tag="OO470_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6253090.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          261 a          251 c          266 g          290 t
ORIGIN      
        1 gccaaattta tcggtgactt tgccaaagcc atgcgcccag aaagtttcct gccaggccat
       61 ttcgattttt ccgttagcgg caaggttatc aaaccagcgt tttccttctt cgacctgttg
      121 cgaatcgagc accagcgtaa agccggagta gctggctttt cctgacggta tagcatcgct
      181 catcatgatg tcgcttccgg caatgcgcac gttggcatga gcgatggcgg tatcgggaaa
      241 ttgcattccg gaagggcagt tctcggcgct gtcctgcgct gatttcggca tttcgccgaa
      301 gctgatttta tagagcagtt ccgcgcccaa cgtacgttga taataggcaa tcgcgtcgga
      361 acagttaccg gcaaaagaga ggtagggact taacggcatg atggtgacct cagttaagag
      421 aagccagtta agtgtagttc gaaatttata cagatgagag gctgcctgat aagacgcgca
      481 agcgtcgcat caggcagtcg gcatcgttgc cggatgcggc gcgagcgcct tatccggctt
      541 accgatcaca atacaatcag ttctttttca caaactcaga tttcagtttc atcggaccaa
      601 aaccgtcgat tttgcaatcg atgttatggt cgccttcaac caggcggatg tttttcactt
      661 tggtgccaat tttcagcatc gaagagctac ctttcacctt cagatcttta atgatggtaa
      721 cgctgtcgcc gtcagccagc agattgccgt tagcatcttt aacgatcagc tcgtcgcttt
      781 cctgtgcagg ttctgcgtcg ttccattcgt aggcacattc cgggcagatg tacatgccgt
      841 tatcttcgta agtgtattcg gagttgcatt ttgggcagtg tggtaatgac atgtggattt
      901 cctcaaaagt cagcagggca aaagcgccaa aaaacggcag attgccgaaa aaggccgcaa
      961 ttatacacaa aatccctgag tttgtcgggg gtactgctga ttttagacga cgatcgtttt
     1021 catttgaaag gaaacgtaaa aagaaaataa tctttttgcc tctattta
//
LOCUS       DAJTUN010000283         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTUN010000283 DAJTUN010000000
VERSION     DAJTUN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.289"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            288..1067
                     /locus_tag="OO470_004037"
                     /pseudo
     CDS             288..1067
                     /locus_tag="OO470_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          320 a          228 c          255 g          264 t
ORIGIN      
        1 acatttttac aacaattatt gcagagaaaa ttaatgctat tggaatgaag agagtaacga
       61 atggctaaac tggctaaccc gaatgacgaa aaattctgga aaacagcacg caatttcgtg
      121 aagtgattat tttcgtccct aaggaaacct cataggatgc ctgcgggtgc ctgagacttt
      181 ctgttttcag gctaaccggg aatcagaaag acgaaggccc cgaccgatat ttctatcaac
      241 tgtactgcac ccattttgtt ggacgatgaa atggaatagc ccctaatatg tcaaagccaa
      301 aatacccttt tgaaaagcgc cttgaagtcg tgaatcacta cttcactact gatgatggtt
      361 acaggatcat ctcggcacgt tttggtgtcc cccgaaccca ggtcaggaca tgggttgccc
      421 tctatgaaaa acatggagaa aaaggtttaa ttcccaaacc taaaggcgtt agtgctgatc
      481 cagagttgcg tattaaggtc gtgaaagctg tgatcgagca gcacatgtcc cttaatcagg
      541 ctgctgctca ctttatgctt gctggtagtg gttctgtagc caggtggctg aaggtctatg
      601 aagagcgcgg agaagctggt ttacgcgcgc tcaagattgg caccaaaaga aacattgcaa
      661 tatcagttga tccagaaaaa gcggcatcag cattggagct gtcaaaagac cgacgcattg
      721 aggatcttga aaggcaagtt cgatttcttg aaacgcggct tatgtatcta aaaaagctga
      781 aagccttagc tcatcccacg aaaaagtgaa agtactcaac gagctaaggc agttttatcc
      841 tcttgatgag cttctcaggg ctgcggagat accgcgcagt acgttttatt accatctaaa
      901 ggctctcagc aagcctgaca agtatgcgga cgttaaaaag cgtattggtg agatttatca
      961 cgagaataga ggccgatacg gataccgtag ggtaacgctg tctcttcatc gagaagggaa
     1021 acagattaac cataaagctg ttcagcgcct gatgggaacc ctctcac
//
LOCUS       DAJTUN010000284         1043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTUN010000284 DAJTUN010000000
VERSION     DAJTUN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.290"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            53..>1043
                     /locus_tag="OO470_004038"
     CDS             53..>1043
                     /locus_tag="OO470_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6253091.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEED"
BASE COUNT          282 a          236 c          251 g          274 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acg
//
LOCUS       DAJTUN010000285         1037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTUN010000285 DAJTUN010000000
VERSION     DAJTUN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.291"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..467)
                     /locus_tag="OO470_004039"
     CDS             complement(<1..467)
                     /locus_tag="OO470_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253092.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGT"
     gene            complement(469..747)
                     /locus_tag="OO470_004040"
     CDS             complement(469..747)
                     /locus_tag="OO470_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253093.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            688..873
                     /locus_tag="OO470_004041"
     CDS             688..873
                     /locus_tag="OO470_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253094.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(915..>1037)
                     /gene="hokD"
                     /locus_tag="OO470_004042"
     CDS             complement(915..>1037)
                     /gene="hokD"
                     /locus_tag="OO470_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6253095.1"
                     /translation="IVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE"
BASE COUNT          233 a          272 c          297 g          235 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaacaggt cgaagtaata cccgcacatt acctccagat
      481 ccgttgctgg aatgtgcagg acggacgcgg tggccgttcg gaataaggga gcctgacgaa
      541 gattatccag tgacgataat cgagactgag ggctttctta atctcgtatc cgtgtctgcg
      601 gtagcgctga atcagccatt cagcctgttc ttcggtgcag gggtcgtgct gatacccgtc
      661 agatttgaat gcatgagaac gccgcccgtg cctgctggca ggggcggcag agttatccga
      721 attgtaaaat ttggtatcgt gcgccatctg ttttctctgc tggcgcagca ggtgccagtt
      781 gttcaggctg acggatggat tgtaaaccag aacgaccaga aaaaacaaaa cccgccgaag
      841 cgggttaagt gcgggtgcgt tgaggatgcc tgacacatca gcggtggcga gggatttctc
      901 ccccgccggg tctcttactc ctcaggttcg taagctgtga agacagcgac ctccgtctgg
      961 ccggttcgga ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt cactatgacg
     1021 gttaaacaga tgacgat
//
LOCUS       DAJTUN010000286         1030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTUN010000286 DAJTUN010000000
VERSION     DAJTUN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.292"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            327..>1030
                     /locus_tag="OO470_004043"
     CDS             327..>1030
                     /locus_tag="OO470_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6253096.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT          344 a          205 c          186 g          295 t
ORIGIN      
        1 agaacttctt cggcacactg caaaatcgca tcgtggtcga tcgctaattc gtcgtgaatc
       61 actgaacgat gttccatgcg aacagatatt cgatcatatc gaaatattaa acattacccc
      121 tccccacatt gggcaaaaaa aaacaaaaaa cgatcaatta acgatcaatt agtattgatt
      181 cacataatct ctcatcatta atgagaacat tgtcacatat ttacaatata gaactgttac
      241 ttttagcaag ctgttagtaa ccaaccgtta ctaataattt taattcttaa tactttgttc
      301 attcaataaa tgagttgaga gaatatatga ataaaatata ctcccttaaa tatagtgctg
      361 ccactggcgg actcattgct gtttctgaat tagcgaaaag agtttctggt aaaacaaacc
      421 gaaaacttgt agcaacaatg ttgtctctgg ctgttgcagg tacagtaaat gcagcaaata
      481 ttgatatatc aaatgtatgg gcgagagact atcttgatct tgcacaaaat aaaggtattt
      541 tccagcccgg agcaacagac gtaacaatca ctttaaaaaa cggagataaa ttctctttcc
      601 ataatctctc aattccggat ttttctggtg cagcagcgag tggcgcagct accgcaataa
      661 gaggttctta tagtgttact gttgcacata acaaaaagaa ccctcaggcc gcagaaactc
      721 aggtttacgc tcagtcttct tacaaggttg ttgacagaag aaattccaat gattttgaga
      781 ttcagaggtt aaataaattt gttgtggaaa cagtaggtgc caccccggca gagaccaacc
      841 ctacaacata ttctgatgca ttagaacgct acggtatagt cacttctgac ggttcaaaaa
      901 aaatcatagg ttttcgtgct ggctctggag gaacatcatt tattaatggt gaatccaaaa
      961 tctcaacaaa ttcagcatat agccatgacc tgttaagtgc tagtctattt gaggtcaccc
     1021 aatgggactc
//
LOCUS       DAJTUN010000287         1029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTUN010000287 DAJTUN010000000
VERSION     DAJTUN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.293"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            207..>1029
                     /locus_tag="OO470_004044"
     CDS             207..>1029
                     /locus_tag="OO470_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6253097.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT          345 a          248 c          149 g          287 t
ORIGIN      
        1 tttttttaca ttaaaccgat ccagtttagg atagtgtaaa gcagaagttc ttcaatatat
       61 aacatatgga gttaatatgc ttacacaaac tatatatccg cctcctccag aagttcctgt
      121 ctactgctga agggaaccaa taacattgca aagcagataa aaaagaatcc aacacctctc
      181 aacataaaga atacgccctg atatttatgc tcccgacaaa taacaatcac agattaattt
      241 caaattcgtt ctccacttat tcaatcgaca ctagccgcgc atatgaaaat tacctaaccc
      301 attggactga atggaaaaat aaccgcatac aagaagaaca acgagacatc gcttttcagc
      361 gactagtatc atgtctacaa aaccaagaga cgaacctgga cttgtctgaa ttaggcctga
      421 caacattacc tgaaatcccc ccgggaatta aatcaattaa tataagtaaa aataatttaa
      481 gcttaatctc cccattacct gcgtccctta cacaacttaa tgtcagctat aacagactta
      541 ttgaactgcc tgctttgcct caaggactta aattattgaa tgcgtcccac aatcaactaa
      601 tcacactacc cacactcccc atatctttga aggagcttca tgtctcaaat aatcaattat
      661 gttctcttcc tgttttacca gaactactgg aaacattaga tgtatcatgt aatgggctgg
      721 cagttttacc acctttacca ttttctttac aagagattag cgcaataggg aatcttctta
      781 gtgaactccc ccctctacct cacaacattc actccatatg ggcaatcgac aatatgttaa
      841 ccgatattcc atacctgccg gaaaatttaa ggaacggtta ttttgacata aatcagataa
      901 gtcatatccc ggaaagcatt cttaatctga ggaatgaatg ttcaatagat attagtgata
      961 acccattgtc atcccatgct ctgcaatccc tgcaaagatt aacatcttcg ccggactacc
     1021 acggcccgc
//
LOCUS       DAJTUN010000288         1015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTUN010000288 DAJTUN010000000
VERSION     DAJTUN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.294"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            16..352
                     /locus_tag="OO470_004045"
                     /pseudo
     CDS             16..352
                     /locus_tag="OO470_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          279 a          221 c          226 g          289 t
ORIGIN      
        1 tctactgatt agattatggt tatcagtaca gtgggttatg aaaaacggac tggatctgcc
       61 agcagtgctt ctattattat tcaaaaagaa cttcagccgg acaatgctat aatccaggga
      121 aaggctataa gctctgttgg acggaatgtg gaaatcagca ctggtaaaaa ggggaactga
      181 cattaacaca agaaggattc ctgcaagcaa ctgcttatct gcccggaacg cagaattaaa
      241 gcataaccac acaggagcca gaatactgtg gtcgtacgca ttcagtattg tgacaactaa
      301 tagaaataaa aatctggccg gtgcgaatat taaacgatgc gataccgtat agtgtaagtc
      361 aaaagtaacg gggtatttaa accatctttt catatttatt taacataatg catattctga
      421 tgtattagtt cagttgttcg ccagaatcag cgctcgatca ctatgcgttt tagcagtgtg
      481 ctgaagaagt tcttcatcac tgcgttattc agaccgtttc ctttgtgcgg catattttgt
      541 gctactccat ggggctttaa ctttgcctat agcctgcgat tcgatattgc caaccctgat
      601 ccatatgcag cgacggggca tcctctggcc gggcactgtg aatgcatcgt atagcatcgt
      661 attaacgatg ctccatcacc gacctttctg acaagctgta ggagataatt tcccggttaa
      721 acagatcgga gaactggcga caggtaccac ttttcccctg taccgagaac tctgtaacat
      781 ccgctcccat ttttcgttgg cctttgtcaa agttccggct caggatattg ggcgcagatc
      841 acttatttgg tggacaccac tttgtcggat gcttcagatt atgaccagat ggatttcgct
      901 ggacgcttac gattacatgc accatattgt caacgattac gccactgagt gtatgacaca
      961 cattaaagcg ttaggctcag gccttattac aatgtcatgc tagtgggctt gtacg
//
LOCUS       DAJTUN010000289         1014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTUN010000289 DAJTUN010000000
VERSION     DAJTUN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.295"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            38..>1014
                     /locus_tag="OO470_004046"
     CDS             38..>1014
                     /locus_tag="OO470_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6253098.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQ"
BASE COUNT          271 a          226 c          269 g          248 t
ORIGIN      
        1 gtcctataac gataattttc gcctcacagg aagcattatg aaaaagatga tgatagccac
       61 actggctgcc gccagcgtgc tgcttgccgt tgcaaatcag gcgcatgctg gcgcgacgct
      121 tgatgccgtt cagaaaaaag gttttgtgca atgcgggatc agtgatggat tacctaggtt
      181 ctcttatgcc gatgcagacg gtaagttttc aggtattgat gttgatgttt gtcgtggtgt
      241 tgccgctgct gtatttggtg acgacacgaa agtgaaatat accccactca cagcaaaaga
      301 acgcttcacc gctttacagt caggggaagt ggatttgctc tcccgtaata cgacctggac
      361 ttcatctcgc gatgccggga tgggaatggc atttaccggc gtcacttatt acgacggcat
      421 tggcttcctg acgcacgata aagcggggct aaaaagcgcg aaagaactgg atggcgctac
      481 cgtctgtatt caggcgggta ctgataccga actcaacgtc gccgactatt tcaaggcaaa
      541 caatatgaag tacacaccag tgactttcga tcgctctgac gaatcagcga aggcactgga
      601 atctggtcgc tgcgatacgc tagcttcgga tcagtcgcaa ctgtatgcct tgcgcatcaa
      661 attaagcaac ccggctgaat ggattgtctt accggaggtt atctctaaag aaccgcttgg
      721 gccggtagtt cgtcgtggcg atgatgaatg gttctcgatt gtacgctgga cgcttttcgc
      781 catgctaaat gctgaagaga tgggcatcaa ttcccagaac gtcgatgaaa aagcggctaa
      841 tccagcaacg cctgatatgg cacatctgtt gggtaaagaa ggcgattacg gcaaggatct
      901 gaagttggat aataaatggg cctataacat catcaaacag gtgggtaact actcggaaat
      961 ttttgagcgt aacgtaggtt cagaaagccc gctgaaaatt aaacgtgggc aaaa
//
LOCUS       DAJTUN010000290         1005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTUN010000290 DAJTUN010000000
VERSION     DAJTUN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.296"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            21..261
                     /locus_tag="OO470_004047"
                     /pseudo
     CDS             21..261
                     /locus_tag="OO470_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     misc_feature    216..332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            258..614
                     /locus_tag="OO470_004048"
     CDS             258..614
                     /locus_tag="OO470_004048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253099.1"
                     /translation="MMPLLDKLREQYGGGPLCSELHIAPSTYYHCQQQRHHPDKRSAR
                     AQRDDWLKKEIQRVYDENHKVYGVKSGVSCYGKVSEWPDALWHVSWRLWDLPVFSGVK
                     RSVRPSAGKPLPQATA"
     gene            497..>1005
                     /locus_tag="OO470_004049"
     CDS             497..>1005
                     /locus_tag="OO470_004049"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6253100.1"
                     /translation="MARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFV
                     AERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALW
                     ARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEV
                     IHRKSWKNRA"
BASE COUNT          239 a          247 c          309 g          210 t
ORIGIN      
        1 cagggcgaat atgactcaca atgggcgaca atttgttcca ttgccccaaa gattggctgt
       61 acgccggaga ctctgcgtgt ctgggttcgc cagcatgagc gggataccgg gggcggtgat
      121 ggagggctca ccaccgctga acgtcagcgt ctgaaagagc tggagcgtga aaatcgtgaa
      181 ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa ggcggagttc
      241 gaccgcctct ggaaaaaatg atgccactgc tggataagct gcgtgagcag tacgggggcg
      301 gaccgctatg cagcgaactg catattgccc cgtcaacgta ttaccactgt cagcaacagc
      361 gacatcatcc ggataaacgc agtgcccgtg cgcagcgcga tgactggctg aagaaagaga
      421 tacagcgcgt atacgatgaa aatcacaagg tatacggtgt aaagtctggc gtcagttgtt
      481 acgggaaggt atcagagtgg ccagatgcac tgtggcacgt ctcatggcgg ttatgggact
      541 tgccggtgtt ctccggggta aaaaggtccg tacgaccatc agccggaaag ccgttgccgc
      601 aggcgaccgc gtaaaccgtc agttcgtggc agaacgacct gaccagctgt gggtggctga
      661 ttttacttac gtcagcacat ggcggggctt cgtctatgtg gcgttcatca ttgatgtgtt
      721 tgccggatac atcgtggggt ggcgggtctc atcgtccatg gaaacgacat tcgtgctgga
      781 tgcactggag caggcgttat gggcccgtcg accgtccggc acggtccatc acagtgataa
      841 aggttctcag tatgtatcgc tggcctacac acagcggctt aaggaagccg gattactggc
      901 atcaacagga agtacaggcg actcgtatga caacgcgatg gcggagagca tcaatggtct
      961 ttacaaagcg gaggtaatac accgtaagag ctggaaaaac cgtgc
//
LOCUS       DAJTUN010000291          989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTUN010000291 DAJTUN010000000
VERSION     DAJTUN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.297"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..967
                     /locus_tag="OO470_004050"
     CDS             <1..967
                     /locus_tag="OO470_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6253101.1"
                     /translation="AEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVDAV
                     SNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEAMVRIDMSEFM
                     EKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDD
                     GRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKELVLGVVSHNFRPEFI
                     NRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDEALKLLSENGYDPVY
                     GARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAVQ"
BASE COUNT          224 a          220 c          294 g          251 t
ORIGIN      
        1 tgctgaagtg ctggcgcgtt ggacggggat tccggtttct cgcatgatgg aaagcgagcg
       61 cgaaaaactg ctgcgtatgg agcaagaact gcaccatcgc gtcattggtc agaacgaagc
      121 ggttgatgcg gtatctaacg ctattcgccg tagccgtgcg gggttggcgg atccaaatcg
      181 cccgattggt tcattcctgt tcctcggccc aacgggtgtg gggaaaactg agctttgtaa
      241 ggcgctggcg aactttatgt ttgatagcga cgaggcgatg gtccgtatcg atatgtccga
      301 atttatggag aaacactcgg tgtctcgtct ggttggtgcg cctccgggat atgtcggtta
      361 tgaagaaggt ggctacctga ccgaagctgt gcgtcgtcgt ccgtattccg tcatcctgct
      421 ggatgaagtg gaaaaagcgc atccggatgt cttcaatatt ctgttgcagg tactggatga
      481 tgggcgtctg actgacgggc aggggagaac ggtcgacttc cgtaatacgg ttgtcattat
      541 gacctctaac ctcggttccg atctgattca ggaacgcttc ggtgaactgg attatgcgca
      601 catgaaagag ctggtgctcg gtgtggttag ccataacttc cgtccggaat tcattaaccg
      661 tattgatgaa gtggtggtct tccatccgct gggtgaacag cacattgcct cgattgcgca
      721 gattcagttg aaacgtctgt acaaacgtct ggaagaacgt ggttatgaaa tccacatttc
      781 tgacgaggcg ctgaaactgc tgagcgagaa cggttacgat ccagtctatg gtgcacgtcc
      841 tttgaaacgt gctatccagc agcagatcga aaacccgctg gcacagcaaa tactgtctgg
      901 tgaattggtt ccgggtaaag tgattcgcct ggaagttaat gaagaccgga ttgtcgctgt
      961 ccagtaatga taaaacgagc ccttcgggg
//
LOCUS       DAJTUN010000292          984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTUN010000292 DAJTUN010000000
VERSION     DAJTUN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.298"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(1..984)
                     /locus_tag="OO470_004051"
                     /pseudo
     CDS             complement(1..984)
                     /locus_tag="OO470_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          253 a          260 c          213 g          258 t
ORIGIN      
        1 atagtttcac ctaactcctg acttaacgtt accccttcgc tgtgtgccag aattccccct
       61 ctgataccat aattcagcct ctgggaattt tggctgtagt tatagctgcc gttcaccgtg
      121 ccataagagc catcataggt cagggacata ttaccgccaa cctcatcatc atgactgtta
      181 taggactgca ttaaattcca tgaaaggtta tttctttcca gcgccgtacc gctgagtccg
      241 agttgatgtg agctaccgga attatcgttt tgcgaaacag aataggtggc gtaactatta
      301 ccccaccagc ccgataaagg aacagataaa gagagcatgt agatctgctc actggtgtta
      361 tcactggcat ggttgttccc cgtcccctgt ctggcagtat tggaataact ccaggagacg
      421 ttccacgaga gacgcttcca gctactgctg tagccaaatt gcattaatcg ttcgacatca
      481 tcggtatgcc agtattgttg ccggcttaat gtcaggtata aattgccata atctctcccc
      541 aacgactgcg ttagcgttcc ctccacgcga ctacgtcggt tacctgtttc ccaaaagtct
      601 tcagggctgt tgcgccgcga tgcccactca ttgagtggta tagtagccct cggtggagta
      661 acgatagcca accaggcgca agctagtgtc tgtgtcgtca aatagttttg aatagagaaa
      721 gcgatatgac tgacccgttt ctgtatcatc atggctaaaa ttcgcacgag catgtgtcac
      781 atcgaaagaa attgcgccca aagcgccaag atctttaccg acacccagag ctgcagaacc
      841 ataattttca gcagcctgca tacctccata taaagtgatc ccgtgggaaa aaccatgaag
      901 tatttgcgcc tgaaggacgt ccggtgtaaa tccgtcttca tcgcgcaatt cacccacgct
      961 gacatcaaca tctgtcagac cttc
//
LOCUS       DAJTUN010000293          973 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTUN010000293 DAJTUN010000000
VERSION     DAJTUN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.299"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            532..>973
                     /locus_tag="OO470_004052"
     CDS             532..>973
                     /locus_tag="OO470_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6253102.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSR"
BASE COUNT          247 a          219 c          213 g          294 t
ORIGIN      
        1 catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc tatctgccgt
       61 tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc gctctcttta
      121 acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt ggcgtaattt
      181 tttaatgatt taattattta actttaattt atctcttcat cgcaattatt gacgacaagc
      241 tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa attaattatt
      301 acttttccta attaatccct caggaatcct caccttaagc tatgattatc tgggcttagg
      361 gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg cgcaaattct
      421 tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa cgatgattcg
      481 tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa agtgaaacat
      541 ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat tgccgctatc
      601 ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga ctttctggag
      661 acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct gttatggata
      721 aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa acgtgaactg
      781 atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc cgctattggt
      841 gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc gattacccgc
      901 gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt actggcgctg
      961 ttgggaagtc gtg
//
LOCUS       DAJTUN010000294          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTUN010000294 DAJTUN010000000
VERSION     DAJTUN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.300"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(201..866)
                     /locus_tag="OO470_004053"
     CDS             complement(201..866)
                     /locus_tag="OO470_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6253103.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          245 a          233 c          215 g          254 t
ORIGIN      
        1 gtaagaggct aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc
       61 ttgccggacg cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct
      121 gaagcagata gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat
      181 cagaaaggtg ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt
      241 ttgataagag ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca
      301 cagccacaat attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac
      361 gagccgccct gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg
      421 gctcgccgcc cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa
      481 ctgctgatca cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg
      541 tcaaaacaca tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt
      601 tcaaaccagt tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca
      661 aagcgaatat tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg
      721 tagaaaaaga tggtgtagtc gattcccgag gcctgaagcg cctccatcac ctcaccggaa
      781 catggagcac agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg
      841 ggccgtttga aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag
      901 agtgtagcat tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTUN010000295          941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTUN010000295 DAJTUN010000000
VERSION     DAJTUN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.301"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..197
                     /locus_tag="OO470_004054"
     CDS             <1..197
                     /locus_tag="OO470_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6253104.1"
                     /translation="LDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPS
                     FATLKKFARATGKKLQIRFV"
     gene            complement(239..415)
                     /locus_tag="OO470_004055"
     CDS             complement(239..415)
                     /locus_tag="OO470_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR6253105.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(412..822)
                     /locus_tag="OO470_004056"
     CDS             complement(412..822)
                     /locus_tag="OO470_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6253106.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
BASE COUNT          212 a          240 c          214 g          275 t
ORIGIN      
        1 tgcttgatga aatgctggca gcacgcaaag aagctggctt aactcaggct gaagttgccg
       61 agcgaatggg aacaaaagcg accgcgatca ccagaatgga aagtaatctc gcatcaggta
      121 tcagcggccc atcatttgcc acactaaaaa aattcgcccg cgctactgga aaaaaactcc
      181 agatccgctt cgtttaacca caccgcgccg tcattctggc ggcgcaccgg ataaatagtc
      241 atttcctcgc acgatgtctt agccaccgga tatcccacag gtgagtcgtg tagttgaagg
      301 tttttacgtc agattctttt gggattggct tgcgtttatt tctggagcgt ttcgttggaa
      361 ggtatttgca gttttcgcag atgatgtcgg tgaaacttcg tcgatgtcgc ctcattcata
      421 cctcctgtcg gtaaatctga caccctgacc aatagcccag gctgttgtgt actcgatcag
      481 acttgccatg cgcttcacgc tcatctgcgc gctgctttcg cgaatgttga cgcattctcc
      541 ttcaaggccg ggcaaaacat cagcttcctg ttttgttgcc actgcatgac cgctgatcaa
      601 caaaaccttc cattgttctg gttttaacca tttgccgcac cattgaacct gacgagcgat
      661 atccgccagc atcgcgtgaa attttgcgtt ctggtcaaga tttcgcttgt agtcagtaat
      721 gcggatggtg actggcttgt ctttatcgag aggagttgag aggatggcat ttattgcggc
      781 ttgctgttgt tgcttagttc ggaggaagat tgtttgcttc atcgaaaatt cttctcttta
      841 attccagcgg ctctgatagc tttcattact gcaattaccg ttttgtcctt cccatcctca
      901 tgccccatcg cataagcacc ttcttcacca tctttccaaa a
//
LOCUS       DAJTUN010000296          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTUN010000296 DAJTUN010000000
VERSION     DAJTUN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.302"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..902
                     /locus_tag="OO470_004057"
                     /pseudo
     CDS             1..902
                     /locus_tag="OO470_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          243 a          230 c          208 g          221 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 ac
//
LOCUS       DAJTUN010000297          886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTUN010000297 DAJTUN010000000
VERSION     DAJTUN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.303"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..723
                     /locus_tag="OO470_004058"
     CDS             <1..723
                     /locus_tag="OO470_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase"
                     /protein_id="HCR6253107.1"
                     /translation="PAFDDFWNNKEYVEFDHPQMFVRHQAFREDPDLEPLGTPSGLIE
                     IYSKTIADMNYDDCQGHPMWFEKIERSHGGPGSQKYPLHLQSVHPDFRLHSQLCESET
                     LRQQYTVAGKEPVFINPQDASARGIRNGDVVRVFNARGQVLAGAVVSDRYAPGVARIH
                     EGAWYDPDKGGEPGALCKYGNPNVLTIDIGTSQLAQAASAHTTLVEIEKYNGTVEQVT
                     AFNGPVEMVAQCEYVPASQVKS"
     gene            720..>886
                     /gene="torD"
                     /locus_tag="OO470_004059"
     CDS             720..>886
                     /gene="torD"
                     /locus_tag="OO470_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6253108.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVN"
BASE COUNT          203 a          228 c          269 g          186 t
ORIGIN      
        1 ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg
       61 tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt
      121 ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg
      181 catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat
      241 ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagct atgtgagtcg
      301 gaaacgctgc gtcagcaata tacggtagcg ggtaaagagc cagtgttcat taacccgcag
      361 gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt
      421 caggtgttgg caggagcagt agtttctgac cgctatgcac ccggcgtggc gcgaattcac
      481 gaaggggcat ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt
      541 aaccccaacg tgctgaccat cgacatcggg acttcgcagc tggcgcaggc ggccagtgcg
      601 cacactacgc tggtggaaat tgagaagtac aatggaacag tggagcaggt gacggcgttt
      661 aacggccccg tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca
      721 tgaccacgct gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct
      781 cccgtgaact ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt
      841 tttcgttgct gaaaagcgaa ccgccgctca ctgcggcggt gaatga
//
LOCUS       DAJTUN010000298          868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTUN010000298 DAJTUN010000000
VERSION     DAJTUN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.304"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..349
                     /locus_tag="OO470_004060"
     CDS             <1..349
                     /locus_tag="OO470_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6253109.1"
                     /translation="QEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLG
                     VLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWR
                     LKIEEESTPEIAA"
     gene            complement(388..>868)
                     /locus_tag="OO470_004061"
     CDS             complement(388..>868)
                     /locus_tag="OO470_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6253110.1"
                     /translation="SVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQIT
                     LQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASIC
                     HLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT          238 a          220 c          196 g          214 t
ORIGIN      
        1 tcaggaacag cagctgatga tggcgacact ggtgcgctat caccgtaaag cgattaagct
       61 cgacgatctg ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac tgatacagct
      121 attgcgcctt ggcgtattac taaacaatca acgtcaggca accaccacac cgccaacatt
      181 gacactgatt actgatgaca gtcactggac actgcgtttc ccgcatgact ggtttagtca
      241 gaatgcgctg gtactgcttg atctggaaaa ggagcaagaa tactgggaag gcgtggctgg
      301 ctggcggttg aaaattgaag aagaaagtac cccagaaatc gccgcttaat gctttgcggg
      361 ccgacgagaa atgtcggccc gcattattca ggcactttcg cgaatgggtt cgatttcatt
      421 cagcgtatca attaacggtt gcggcttacc aataagataa ccctgcatat aatcgatccc
      481 caaagagagc accgcctcgc ggatctcttc gttttcaacg tactctgcca ctacccgcat
      541 tttcttcatt cgcgccagat ggcaaatcga cgccacaatc tgataatcca gactatttga
      601 cacaatattg cggataaagc tgccgtcaat tttaagcaga tcggcattca catttttaag
      661 ccgtgcatag ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc agcctaattc
      721 ctgaagatgc tgcaaggtga tttgcgcctg cttaacattg gtcagagcat tactttcggt
      781 gacttcaaaa ataagttgcc acgcttcaat ctgatattta gccagcaact gactgacttc
      841 aacaggaaaa cgagcctgac ataccgag
//
LOCUS       DAJTUN010000299          862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTUN010000299 DAJTUN010000000
VERSION     DAJTUN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.305"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..422
                     /locus_tag="OO470_004062"
     CDS             <1..422
                     /locus_tag="OO470_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6253111.1"
                     /translation="DYASHPQHFNHMVEAFRRDLKQYHSQLNNITDAPWFCGDTTWYW
                     KENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPDDLSTGYYGSAYRS
                     PENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          245 a          189 c          195 g          233 t
ORIGIN      
        1 gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt cgtagggatc
       61 taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt tgcggcgata
      121 ccacctggta ctggaaagaa aatttcccgc attcgtatga agctatttat ggcaattatc
      181 aaaataatgt tttagccaat attattttcg tcgacttcca gcaacaaggt gaaagaggac
      241 tgacgaacgc gcctgatgaa gacccggacg atttaagcac gggatattac ggttcagcgt
      301 accggtcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc acggcagccc
      361 gtcgggggat tatttctgac agatttgtag aagcaatttt gcagttttgg cgcgaaaggt
      421 gagcggggag tttattcatg ggggatgtcc ttgttcgttc tcagagtaga caacaaaaaa
      481 caaatgagat caaggtaata tctcttcact tgcgggctcg aacagcaggc aattgccaat
      541 ataataatct ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt attgcttaca
      601 gattagccgg atgccttaat agacatccgc gccttttcga ttatggctcg gaaatctacg
      661 aacatctaca cagtttactg gaacgttttg ggccacagcg ttcgcagcac cgtcctgata
      721 tgccgttctg gcgattataa gtcgacggat tctggcaaat agacgatgat gatatgctta
      781 atgatgttca aagaaattag gccattatta tattattgct tgcattttct ttcattccta
      841 aaagcacccg ccattaatcc ca
//
LOCUS       DAJTUN010000300          853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTUN010000300 DAJTUN010000000
VERSION     DAJTUN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.306"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            45..>853
                     /gene="adrA"
                     /locus_tag="OO470_004063"
                     /gene_synonym="dgcC"
     CDS             45..>853
                     /gene="adrA"
                     /locus_tag="OO470_004063"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6253112.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVA"
BASE COUNT          175 a          207 c          245 g          226 t
ORIGIN      
        1 ctgcctttag ccccgtctct ataatttggg aaaattgttt ctgaatgttc ccaaaaataa
       61 tgaatgatga aaactttttc aaaaaagcgg cggcgcatgg ggaggaacct cctttaactc
      121 ctcaaaacga acatcagcgg tccgggctgc gcttcgcccg tcgcgtcaga ctaccccgtg
      181 cggttggcct ggctggcatg ttcttaccga ttgcttcaac gctggtttcg cacccgccgc
      241 cgggctggtg gtggctggtg ttggtcggct gggcgttcgt ctggccgcat ttagcctggc
      301 agatagcgag cagggccgtc gatccgctta gccgggaaat ttacaactta aaaaccgatg
      361 cagtattagc gggaatgtgg gtaggcgtaa tgggcgtaaa cgtgctgcct tccaccgcta
      421 tgttgatgat tctgtgtctg aatttgatgg gggcaggcgg cccccgtctg tttgtcgcgg
      481 gtctggtgtt gatggtggtt tcctgccttg tcaccctcga gcttacgggc attaccgtgt
      541 cgttcaatag tgctccgctg gaatggtggc tctcccttcc cattattgtc gtttatcctc
      601 tgatgtttgg ctgggtcagc taccagacgg caaccaaact ggcggaacat aaacgcaggt
      661 tgcaggtcat gagtactcgc gacggcatga cgggcgtgta taaccgacgt cattgggaaa
      721 ctatgttacg caatgaattt gataactgtc ggcggcataa tcgcgatgca acgttactga
      781 ttatcgatat cgaccatttc aagagcatca acgatacctg gggtcatgat gtgggcgatg
      841 aagcgattgt ggc
//
LOCUS       DAJTUN010000301          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTUN010000301 DAJTUN010000000
VERSION     DAJTUN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.307"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..320
                     /locus_tag="OO470_004064"
     CDS             <1..320
                     /locus_tag="OO470_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine utilization protein EutP"
                     /protein_id="HCR6253113.1"
                     /translation="YHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVI
                     SKTDMPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTH
                     HSE"
     gene            295..>829
                     /locus_tag="OO470_004065"
     CDS             295..>829
                     /locus_tag="OO470_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6253114.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGR"
BASE COUNT          205 a          233 c          238 g          153 t
ORIGIN      
        1 ggtatcacgc cttaattacc acgctacagg atgtagacat gctgatctat gtccatggcg
       61 caaatgaccc ggaaagtcgg ttgcctgccg ggttgctgga tattggcgtc agcaaacggc
      121 aaatcgccgt catcagtaaa acggacatgc cagatgctga cgtcgccgca acgcgaaagt
      181 tattgcttga gacgggtttc gaagagccga ttttcgagct aaacagccac gacccgcaaa
      241 gcgtgcagca actggtggat tatctggcat ctctcaccaa acaggaggaa gcaggtgaaa
      301 aaactcatca cagcgaatga tattcgtgaa gcacacgcgc gcggcgaaca ggcaatgtcc
      361 gtggttctgc gcgccagcat tattaccccg gaagcccgtg aagtggcgga tctactgggt
      421 tttaccatca ccgaatgtga tgaatcgatc ccggtaacgg cgtccgtgcc cgccagcgtg
      481 ccagccgaca aaaccgaaag ccagcgcatc cgcgaaacca tcattgccca gctgccggaa
      541 ggccagttta ccgaaagcct ggtcgcacag ctgatggaaa aagtgatgaa ggaaaaacag
      601 tcgctgggcg attttgtcgt cgccagcgcc gggccgcacc tcgaatccgt gatcgccggt
      661 cacggagccg gggcgcaaac cctttctgaa caacggctgt gtcgggtact gaatatcgat
      721 atcggcggtg gcaccgcgaa ctacgccctg ttcgatgccg gaaaaatcag cggcactgcc
      781 tgtctcaacg tcggtggtcg cctgctggaa accgacagcc aggggcgcg
//
LOCUS       DAJTUN010000302          813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTUN010000302 DAJTUN010000000
VERSION     DAJTUN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.308"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..414)
                     /locus_tag="OO470_004066"
     CDS             complement(<1..414)
                     /locus_tag="OO470_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6253115.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
     gene            complement(411..>813)
                     /locus_tag="OO470_004067"
     CDS             complement(411..>813)
                     /locus_tag="OO470_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6253116.1"
                     /translation="FASGMEKAMKQILGDQSQFIAIDFPETSSAALLTSQLEEAIAQL
                     DCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVLEREGLSGEEFR
                     VQALECGHRGLTSLVDELGRCHEECPVEEGI"
BASE COUNT          170 a          251 c          206 g          186 t
ORIGIN      
        1 cggatgcgcg cctttattct gcggcgtgaa gtacggtcct tcgagataac tccccagcac
       61 ttgcgcacca ggtccgccgc gttggcaacg ttgagcaata cgtttcagcg ccgcatgaat
      121 ggtattaagc ggcgcggtta cggtggtcgg taaccaactg ccgacacctt cgcgtgcctt
      181 gtgcattgcc agcttgtcga gtacatccgg cgcgtcatcc attacatcaa cgcccgcacc
      241 accgtgtaca tgggtgtcga tgtaagcggg gcagagcagt tccgcatcgc gttcagtcac
      301 gcccactgga atcggttcga ttgctgcgat gacaccgtca gcaacacgca actgatggtc
      361 atcgaaccat ccctcttcag tcagcagcct tctggcgcgc agaacgtgtg tcatattcct
      421 tcctcgaccg gacattcttc atggcagcgg cctaactcgt ccaccagact ggtcagccct
      481 cggtgtccac actccagcgc ctgtacacgg aattcttcac cgcttaaccc ttcgcgctcc
      541 agcaccatct ccagtagcag ttgcaaattg gtgccggtaa tcacttcaca gcccggtttt
      601 tgcatcgcca gcgttgaagc tacgcgaaac ggcgtgccgc ccagcaaatc cgtcagaaaa
      661 acaatgccgt cttcacaatc cagttgggcg atggcctctt caagctgcga tgtaagcagc
      721 gcggcgcttg aggtttcggg aaaatcgatg gcaataaact gcgattgatc gccgaggatt
      781 tgcttcattg ctttttccat gccactggca aac
//
LOCUS       DAJTUN010000303          811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTUN010000303 DAJTUN010000000
VERSION     DAJTUN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.309"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>811
                     /locus_tag="OO470_004068"
     CDS             <1..>811
                     /locus_tag="OO470_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6253117.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAJTUN010000304          792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTUN010000304 DAJTUN010000000
VERSION     DAJTUN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.310"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..610)
                     /gene="ipaH3"
                     /locus_tag="OO470_004069"
     CDS             complement(<1..610)
                     /gene="ipaH3"
                     /locus_tag="OO470_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6253118.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNH"
BASE COUNT          236 a          123 c          187 g          246 t
ORIGIN      
        1 gatgatttct tacaggtacg gctggtaggc tttccagaag attagtactt acatcgagcg
       61 cttccagtga ttcaggtaac tcagggagaa atgtcagctg gttatttctt actgagagca
      121 cttccagcga tgtaggtaat tcaggaagca tggttagctg attgttatct gcattaatat
      181 attccagcaa tgcaggcaat tcaggaagca tggttagttg gttgttatct acatcaagat
      241 gtttcagaga tgcgggtaat tcaggaagtg ttgacaggcg attgtcacag gcgtcaaggt
      301 attccagcga tgctggcaat tctggtaatg atattagggc attctgagta atttccagaa
      361 cagtgatttg aggtggtaag ttgtcaggta gcgaggacag atttaaacga ttcagttgaa
      421 gctcactgaa ctgattgatg agacattctt taagtaggga gaccgcttca ttccgatttt
      481 caccggggag cgcttgtttt tcccatttat cccatgctga aaagtaatca gcatatgtac
      541 cggaaatagt gttataaaaa gaattttggg acaatgaaaa gttattattt atcggtaaca
      601 tgatggacat tcagttaaag ccgatggaca aatggctttg ttaaaagaaa taagtaggag
      661 tttttatgaa gttcattcca tgaaatgaac ttcagcactt ttttattata aaaggacaaa
      721 caaaattggc tgtaaaaaaa cgatactgtt tcagttaact gtgaatagct tgcttgcacc
      781 gtattgttct tg
//
LOCUS       DAJTUN010000305          788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTUN010000305 DAJTUN010000000
VERSION     DAJTUN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.311"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..494
                     /locus_tag="OO470_004070"
     CDS             <1..494
                     /locus_tag="OO470_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6253119.1"
                     /translation="MTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYD
                     WIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKN
                     YLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFS
                     SVK"
     gene            527..788
                     /locus_tag="OO470_004071"
                     /pseudo
     CDS             527..788
                     /locus_tag="OO470_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
BASE COUNT          210 a          192 c          220 g          166 t
ORIGIN      
        1 aaatgactca ggaagcgctc agacggatag cagagttata cgacatagaa gcggagatac
       61 gtggcagtcc ggcagaggaa cggcttgcag tcagaaaagc cagaagcgtc cagttgatgc
      121 agtcgttgta cgactggata cagttgcaga ggaaaacgct gtcgaaacat gcggagatgg
      181 cgaaggcgtt cgactatatc ctgaatcact ggaatgcgct gaacgagttc tgtcgtgacg
      241 gctgggtgga aatagacaac aacatcggtg aaaacgcgtt acgatcggtg gcggttggaa
      301 gaaaaaatta tctctttttc ggctcagaca agggaggaga aagtgcggcg atcatctaca
      361 gtctgctggt cacctgcaaa cagaacgaag tggagccgga ggactggttg cgcgaagtga
      421 tcgagaagct caatgactgg ccgtcgaacc aagtgcatga actgctgccc tggaacttct
      481 cgtctgtaaa ataatcctta cgctacgtac ttctcggggc gcttacaatg aaatcgcttt
      541 cccggcgaac actccggtgt acttcaaagt gacctccaac tccgtgatga actccttctt
      601 cattccgcgt ctgggtagcc agatttatgc catggccggt atgcagactc gcctgcatct
      661 gatcgccaac gaacccggta cttatgacgg tatctccgcc agctacagcg ggccgggctt
      721 ctcaggcatg aagttcaaag ctattgcaac accggatcgc gccgaattcg accaatgggt
      781 cgcaaaag
//
LOCUS       DAJTUN010000306          787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTUN010000306 DAJTUN010000000
VERSION     DAJTUN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.312"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>787
                     /locus_tag="OO470_004072"
     CDS             <1..>787
                     /locus_tag="OO470_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6253120.1"
                     /translation="SASVQAAEIYNKDGNKLDVYGKVKAMHYMSDNASKDGDQSYIRF
                     GFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLG
                     ALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGK
                     NENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWAT
                     GLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF"
BASE COUNT          234 a          171 c          199 g          183 t
ORIGIN      
        1 catctgcatc tgtacaggct gcagaaatat ataataaaga cggtaataaa ctggatgtct
       61 atggcaaagt taaagccatg cattatatga gtgataacgc cagtaaagat ggcgaccaga
      121 gttatatccg ttttggtttt aaaggtgaaa ctcaaattaa cgatcaactg actggttatg
      181 gtcggtggga agcggagttt gccggaaata aagcggagag tgatactgca cagcaaaaaa
      241 cgcgtctcgc ttttgccggg ttgaaatata aagatttggg ttctttcgat tatggtcgta
      301 acctgggcgc gttgtatgac gtggaagcct ggaccgatat gttcccggaa tttggtggcg
      361 attcctcggc gcagaccgac aactttatga ccaaacgcgc cagcggtctg gcgacgtatc
      421 ggaacaccga cttcttcggc gttatcgatg gcctgaactt aaccctgcaa tatcaaggga
      481 aaaacgaaaa ccgcgacgtt aaaaagcaaa acggcgatgg cttcggcacg tcattgacat
      541 atgactttgg cggcagcgat ttcgccatta gtggggccta taccaactca gatcgcacca
      601 acgagcagaa cctgcaaagc cgtggcacag gcaagcgtgc agaagcatgg gcaacaggtc
      661 tgaaatacga tgccaataat atttatctgg caactttcta ttctgaaaca cgcaaaatga
      721 cgccaataac tggcggcttt gccaataaga cacagaactt tgaagcggtc gctcaatacc
      781 agtttga
//
LOCUS       DAJTUN010000307          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTUN010000307 DAJTUN010000000
VERSION     DAJTUN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.313"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>747
                     /locus_tag="OO470_004073"
     CDS             <1..>747
                     /locus_tag="OO470_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR6253121.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DAJTUN010000308          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTUN010000308 DAJTUN010000000
VERSION     DAJTUN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.314"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(315..>726)
                     /gene="trpA"
                     /locus_tag="OO470_004074"
     CDS             complement(315..>726)
                     /gene="trpA"
                     /locus_tag="OO470_004074"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6253122.1"
                     /translation="VEESAPFRQAALRHNVAPIFICPPNADDDLLRQIASYGRGYTYL
                     LSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPDQVKAAIDAGAAGAISG
                     SAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT          197 a          147 c          191 g          191 t
ORIGIN      
        1 ttagtcgcta atgagaatta gtcaaattaa gcgcaacgag aagatagagg gaaaatatat
       61 tttgaggaac attctggata tattaacaat tacctgagga atatgtgact tagagaaaat
      121 tattgatttt actggtgtta tgttgcggga ttaatttgtt tgattatatt actgttgggc
      181 ggaaaaatga cgtaagttga cgttcgaccg gggtaagcga aacggtaaaa agataaatat
      241 taaatgaatt taggattttt ccggcttcat taaagaaagt taaaatgccg ccagcggaac
      301 tggcggctgt gggattaact gcgcgtcgcc gctttcatcg gttgtacaaa aactttcagt
      361 gccgccagca ttttctctgg ctcattaata tgttgctcga tgattttaac aatggccgaa
      421 ccagaaatcg cgcccgcagc tcctgcatca atcgctgctt ttacctgatc cggggcggaa
      481 ataccaaacc cctgcaatgg aggtgcagcg ttgtactctt tcagcttcgc aaccagatga
      541 ttgaggggta acgcggcgcg gttttctgcg ccggtcacgc ccgctcgtga cagcaaatat
      601 gtgtaaccac gaccgtaaga ggctatctgg cgcagcaggt cgtcatcggc atttggcggg
      661 cagatgaaga taggtgcgac attatggcgc aacgcggcct ggcggaaggg cgcggactct
      721 tcaact
//
LOCUS       DAJTUN010000309          723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTUN010000309 DAJTUN010000000
VERSION     DAJTUN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.315"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            137..352
                     /locus_tag="OO470_004075"
     CDS             137..352
                     /locus_tag="OO470_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253123.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            455..723
                     /locus_tag="OO470_004076"
                     /pseudo
     CDS             455..723
                     /locus_tag="OO470_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515373.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          198 a          161 c          174 g          190 t
ORIGIN      
        1 gggtttttgt ttttattttc aacgcatttg aagttctgga tggtgccgga atagaatcaa
       61 aaatacttaa gtagcgcgca gggataaaag ggatggaccc cgaacagggg agtgctactt
      121 atctggaagg attctgatga tgaacatcga agaactgcgt aaaatttttt gtgaagatgg
      181 cctttatgct gtgtgcgttg aaaatggaaa tattgttagt cattaccgca ttgtgtgttt
      241 gcaaaaaaat ggggctgcgt taattaattt tgtggatgcc cgagtgacgg acggatttat
      301 cttgcgcgac ggtgagtttg tcacttcatt acaggcattg aaagagatct gaataaaagc
      361 tggcttttct gctttttcag aagaataaac tcatctacaa tcttgcgcgg ggctgaactc
      421 ccgctgagta acaccgtgcc accggagaaa accgatggca cgcaacgcaa aatattacaa
      481 tcatgataat tcgaccgttc ttgcccacac gcacgagcgg tattctcacg catttaagtc
      541 agactggtac cagcatccct catgcactga agaacaggcc gaatggctta ttcagtgtta
      601 ccgcaggcgc ggatgcgagg ttaaaaaagc ccttagcctc gactaccgtc actagataat
      661 ctccgtcagg ctcccttact ccgaacagcc accgcgtccg tcccgcacat tccagcaacg
      721 gat
//
LOCUS       DAJTUN010000310          722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTUN010000310 DAJTUN010000000
VERSION     DAJTUN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.316"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>722
                     /locus_tag="OO470_004077"
     CDS             <1..>722
                     /locus_tag="OO470_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6253124.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGG"
BASE COUNT          170 a          173 c          213 g          166 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 gg
//
LOCUS       DAJTUN010000311          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTUN010000311 DAJTUN010000000
VERSION     DAJTUN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.317"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            180..>710
                     /gene="kptA"
                     /locus_tag="OO470_004078"
     CDS             180..>710
                     /gene="kptA"
                     /locus_tag="OO470_004078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6253125.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTV"
BASE COUNT          207 a          150 c          194 g          159 t
ORIGIN      
        1 tatcagccat gtgggtatgg ggaaaatgca agtatgtttc tggctatgcg ttttttgcat
       61 aggggaaaaa ttacgctgtg tggaaaaggt tctgcgcagg attgagttgc agaaaattca
      121 taaacccagt gacaatgaca ttagtaaata aaaacagaaa atccagggac gaaaacgcga
      181 tggcaaagta caacgaaaaa gagctggcgg atacgagcaa gtttttaagt tttgtcctcc
      241 gacacaaacc ggaggcgatt ggtatcgtac tggaccgtga aggatgggcg gatatcaata
      301 agctcatact ttgtgcccaa aaagcgggca agcgattaac ccgtgcattg ctggataccg
      361 tggtcgcaac cagcgataaa aagcgtttta gttattccag tgatggtagg tgcatccgcg
      421 cggttcaggg gcattcgact tcgcaggtcg ctatttcctt tgccgaaaaa acaccgccac
      481 agtttctcta ccacggtacg gcaagccgtt tccttgacga gataaaaaaa caggggctga
      541 ttgcagggga tcgtcattac gtgcatctct ctgctgatga agcaacggca cgcaaagtgg
      601 gagcaagaca cggatcgccg gttgtcttaa ccgtcaaagc acaggaaatg gcgaaacgag
      661 gcatcccttt ctggcaagcg gaaaacgggg tctggctgac atcaacggtt
//
LOCUS       DAJTUN010000312          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTUN010000312 DAJTUN010000000
VERSION     DAJTUN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.318"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(22..>695)
                     /locus_tag="OO470_004079"
     CDS             complement(22..>695)
                     /locus_tag="OO470_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6253126.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT          183 a          205 c          176 g          131 t
ORIGIN      
        1 caagagtttg cgtcgtattt gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg
       61 cagagcaaat gccaccagcg ccgggatgag ataaataaac cagacggcga cttcgctgac
      121 gctcggtgct tcctgatagc caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt
      181 tgagaggacc gcgctcatat cgaaggcgat ttcctgaaag tggttccaca atccggcttc
      241 atgaaatgcg cgaatggcac cagccgccag ccctgcggcg acgaagagaa taaacaggct
      301 ggtccattta aaaaatgcac caagattgag gcgaataccg ccccagtaga gcaggaagcc
      361 gagcaccacg gcagtagcaa gaccgagcat tgcacccagc ggcggccaga tcccgacatc
      421 ttgttgaaat gccgccagca ggaaaaagac cgactccagc ccttcccttg caacggcaaa
      481 aaagaccatc atcaccagcg cccagccatg atgatttcca cgctgcaatg cgctatcgac
      541 tgcctgttcc agttgcactt tgacgttgcg cgacactttg cgcatccaga aaaccatcca
      601 ggtaaggatc accacggcga tcaccgccac gataccttca aacagttcct gctctttttg
      661 cggaaattcg ccggtggttt cgttaatgaa gatcc
//
LOCUS       DAJTUN010000313          679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTUN010000313 DAJTUN010000000
VERSION     DAJTUN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.319"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(6..679)
                     /gene="yjfF"
                     /locus_tag="OO470_004080"
                     /pseudo
     CDS             complement(6..679)
                     /gene="yjfF"
                     /locus_tag="OO470_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
BASE COUNT          187 a          201 c          158 g          133 t
ORIGIN      
        1 catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg attctcccac
       61 agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc gatggcgatt
      121 ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat cagtccctga
      181 gttgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa caacgtaccg
      241 ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag tgcatatccg
      301 gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt ggagagcata
      361 taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga cgtcgcgttg
      421 ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa aataccgata
      481 accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc ggggattttc
      541 cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga ctcttccgaa
      601 acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat aaatgccggg
      661 atcttcaggg cgtcgatca
//
LOCUS       DAJTUN010000314          667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTUN010000314 DAJTUN010000000
VERSION     DAJTUN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.320"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..667
                     /gene="ppnN"
                     /locus_tag="OO470_004081"
                     /gene_synonym="ygdH"
     CDS             <1..667
                     /gene="ppnN"
                     /locus_tag="OO470_004081"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6253127.1"
                     /translation="IMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPAN
                     KDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMPL
                     VKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAFS
                     GIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCYE
                     ICT"
BASE COUNT          163 a          167 c          174 g          163 t
ORIGIN      
        1 catcatgccg gatatcgaaa aacgtctgga agcgtttgtc cgtatcgctc acggcatcat
       61 tatcttccct ggcggtgtgg gtacggcaga agagttgctg tatttgctgg gaattttaat
      121 gaacccggcc aacaaagatc aggttttacc attgatcctc accggcccga aagagagcgc
      181 cgactacttc cgcgtactgg acgagtttgt cgtacatacg ctgggcgaaa acgcgcgccg
      241 ccattaccgc atcatcattg atgacgccgc tgaagtcgct cgtcagatga aaaaatcgat
      301 gccgctggtg aaagaaaatc gccgtgatac aggcgatgcc tacagcttta actggtcaat
      361 gcgcattgcg ccagatttgc aaatgccgtt tgagccgtct cacgagaata tggctaatct
      421 gaagctttac ccggatcaac ctgttgaagt gctggctgcc gacctgcgcc gtgcgttctc
      481 cggtattgtg gcgggtaacg taaaagaagt cggtattcgc gccattgaag agtttggtcc
      541 ttacaaaatc aacggcgata aagagattat gcgtcgtatg gacgacctgc tacagggttt
      601 tgttgcccag catcgtatga agttgccagg ctcagcctac atcccttgct acgaaatctg
      661 cacgtaa
//
LOCUS       DAJTUN010000315          664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTUN010000315 DAJTUN010000000
VERSION     DAJTUN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.321"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>664
                     /locus_tag="OO470_004082"
     CDS             <1..>664
                     /locus_tag="OO470_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6253128.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NG"
BASE COUNT          164 a          165 c          194 g          141 t
ORIGIN      
        1 aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca gctaacggta ggcgtggtcg
       61 atgaaatgat ctacgcgctg caaccagaaa tcgcgccgaa tatcggcaaa tttttctatc
      121 cgctggggcg taacaatgtg cgtaccagct ccagtctgtc gtttatcagc tatgaccagg
      181 cgctctctag cgagccggtt gcgcctggcg cgactaaccg cagcgagcgg cgagtaaaaa
      241 tgcctgaacg tccacggcgt gaagaggtgg ataccgcggc atggatgccg tcactcacaa
      301 ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta acccgctggc
      361 gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc
      421 gttcggaaaa aaatctctac atgaagtgga gtatgccaac ggtgtatcgc gtgggcgaca
      481 aaccggcggc aggactgttt atcttcagtc agcaggataa cgaaccggtg gcgctggtga
      541 ctaaatttgc aggcgctgag atgcgccaga cgctgacgct gcacaaaggg gcgaattata
      601 tttcgctgac gcagaatatt cagcaatctg gcttgttaag tgcagaactg caacaaaatg
      661 ggca
//
LOCUS       DAJTUN010000316          662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTUN010000316 DAJTUN010000000
VERSION     DAJTUN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.322"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..332
                     /locus_tag="OO470_004083"
     CDS             <1..332
                     /locus_tag="OO470_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6253129.1"
                     /translation="PENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRGIDT
                     IVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARVRSV
                     EEILHAL"
BASE COUNT          186 a          162 c          145 g          169 t
ORIGIN      
        1 tgcccgaaaa ctggtggcaa catccagctg cattaggtgc aactgacagt gatatcgaaa
       61 tcatcaaacg tcagtggggt gcgttttacg gtacggatct ggagttgcaa ctgcgccgcc
      121 gaggtatcga tacaatagtg ttatgtggga tctcgaccaa tatcggtgtt gaatccaccg
      181 cccgcaatgc ctgggaactt ggctttaatc tggtgattgc cgaagacgcc tgtagtgccg
      241 ccagcgccga gcaacacaat aacagcatta atcatatcta cccgcgcatc gcccgtgtgc
      301 gtagcgtgga agagatcctt cacgcgttat gatttacatc ggtctaccgc aatggtcaca
      361 tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag tatgcccgcc actttaactg
      421 cgtgatacgg gatttttaaa aatcactaaa gaacgcccaa gagcatgtgt tttctttaga
      481 ataatcaaag cgttggattg ccatgtcata tgaaaatcgg caaagttcat atgtaacaaa
      541 gtgtcccaat catgccccaa aatgccccac aacaaatatt tttgccccat gcatgcccca
      601 aaaaatcact ttccctcact cttgatactg tttatccata cagttaaaaa taatactgta
      661 ta
//
LOCUS       DAJTUN010000317          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTUN010000317 DAJTUN010000000
VERSION     DAJTUN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.323"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            258..>657
                     /gene="mcbR"
                     /locus_tag="OO470_004084"
     CDS             258..>657
                     /gene="mcbR"
                     /locus_tag="OO470_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6253130.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIIN"
BASE COUNT          202 a          135 c          162 g          158 t
ORIGIN      
        1 ggcctgctga agggtaaaaa cttcggcaaa gtggtgatcc gcgtcgcggg tgatgattaa
       61 agtttcaacg gcggcgtaag ccgccataaa aatccaggca aaaagcttat tttcagcttt
      121 aattaactaa ccacgtcatt gttctgctgc taataacaac aaaggtgggt ggtttatgat
      181 tattcacttt aatacactag gtgaactcct tctgccatgc aggcagggtt ggacagaaaa
      241 cacgcgaagg aattaaaatg cctggaacgg gaaaaatgaa acatgtcagt ttgactctgc
      301 aggttgagaa cgacctgaaa catcagctta gtattggcgc attaaaacct ggcgcacgcc
      361 tgattactaa aaatctggcg gagcaattag gtatgagtat tacacctgtg cgtgaagcat
      421 tattacgtct ggtttcggtg aatgcgcttt ctgtcgcacc tgcacaagca tttacagtac
      481 cggaagtgag gcaaagtcag ttagacgaaa tcaatcggat ccgctacgaa ctggaattaa
      541 tggcagttgc cctggctgtt gaaaacctct cctcgcaaga catagcagaa cttcaggaat
      601 tgctcgggaa attacagcag gcgcaagaaa agggcgacat ggaacaaatc attaatg
//
LOCUS       DAJTUN010000318          646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTUN010000318 DAJTUN010000000
VERSION     DAJTUN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.324"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..313
                     /locus_tag="OO470_004085"
     CDS             <1..313
                     /locus_tag="OO470_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6253131.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            470..>646
                     /locus_tag="OO470_004086"
     CDS             470..>646
                     /locus_tag="OO470_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HCR6253132.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGEL"
BASE COUNT          172 a          164 c          144 g          166 t
ORIGIN      
        1 ggcgctggac aatgtgttac aggagctggg gcgcgaacgc acgattgcta tgagcctgcc
       61 ggaattcgag cagtcactgt ttatggcagc gcaacccgac aatctgctac tggcgaccgc
      121 gccgcgctac tgtcagtact acaatcaact ccatcaactg ccgttggttg ctcttcctct
      181 cccgtttgac gaaagccagc aaaaaaagct ggaagttcct tttaccctgc tgtggcataa
      241 acggaacagc cataatccga agatcgtctg gttacgggaa accattaaaa acctttacgc
      301 gtcgatggca taaccgaatc gtatgaaacg cgacccaatt tcacaataaa atgtaaaaaa
      361 gttgtaataa gcttgtctga atcgaacttt tagccgcttt agtctgtcca tcattccagt
      421 aaatgattac tcttgtattc ataatggacc attaagcatg gagcgaaaaa tggcgactca
      481 ctttgcccga gggattttaa cggaaggaca tctgatttct gttcgtctcc cctcccagtg
      541 tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg cgtccagagg
      601 tttactcgca gaactgatgt tcatgctgta tggcattggc gaattg
//
LOCUS       DAJTUN010000319          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTUN010000319 DAJTUN010000000
VERSION     DAJTUN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.325"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..587
                     /locus_tag="OO470_004087"
     CDS             <1..587
                     /locus_tag="OO470_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR6253133.1"
                     /translation="EYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKL
                     NRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGD
                     FIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQ
                     FDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT          156 a          182 c          177 g          127 t
ORIGIN      
        1 tcgaataccg ccgtggttac aagttctcca cctacgcaac ctggtggatc cgtcaggcga
       61 tcacccgttc tatcgcggat caggcgcgca ccatccgtat tccggtgcat atgattgaga
      121 ctatcaacaa actcaaccgt atttctcgcc agatgctgca agagatgggc cgtgaaccga
      181 cgccggaaga actggctgaa cgtatgctga tgccggaaga caagatccgc aaagtgctga
      241 agatcgccaa agagccaatc tccatggaaa cgccgatcgg tgatgatgaa gattcgcatc
      301 tgggggattt catcgaggat accaccctcg agctgccgct ggattctgcg accaccgaaa
      361 gcctgcgtgc ggcaacgcac gacgtgctgg ctggcctgac cgcgcgtgaa gcaaaagttc
      421 tgcgtatgcg tttcggtatc gatatgaaca ccgaccacac gctggaagaa gtgggtaaac
      481 agttcgacgt tacccgcgaa cgtatccgtc agatcgaagc gaaggcgctg cgcaaactgc
      541 gtcacccgag ccgttctgaa gtgttgcgta gcttcctgga cgattaatcg gtaggccgga
      601 tcaggcgtta cgccgcaccc ggcactagtc tctctgcaca aa
//
LOCUS       DAJTUN010000320          641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTUN010000320 DAJTUN010000000
VERSION     DAJTUN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.326"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..486
                     /locus_tag="OO470_004088"
     CDS             <1..486
                     /locus_tag="OO470_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6253134.1"
                     /translation="HDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQHLSETKN
                     QIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYVFEQFEIT
                     CYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSETDGVEAK
                     K"
     gene            complement(546..>641)
                     /locus_tag="OO470_004089"
     CDS             complement(546..>641)
                     /locus_tag="OO470_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6253135.1"
                     /translation="TTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          193 a          137 c          136 g          175 t
ORIGIN      
        1 catgactggt tacgtgacgc ccacgcaatg gaaaagcaag ccgaatctat gcttgaatcc
       61 atggccagcc gtatagataa ttatcctgaa ctacgcgctc gtattgaaca acatcttagt
      121 gaaaccaaaa accagattgt tcaactggaa actattcttg atcgtaatga catttcacgt
      181 tcagtcatta aagattccat gagtaaaatg gctgcgcttg ggcagtcaat cggtggtata
      241 ttcccttctg atgaaatagt taaaggctct attggcggat atgtcttcga gcaatttgaa
      301 atcacctgtt acacctcact attagcagca gcaaaaaatg ccggtgatac agtctcaatt
      361 ccgatcatcg aagcgatttt aaatgaggaa aagcaaatgg ccgactggct gattcagcat
      421 attccgcaaa caactgagaa atttttaatt cgttctgaaa ctgatggcgt agaagcgaag
      481 aaataatacc ctttatacca tgtccttatt gaccccgtat attacggggt cgtttttgtg
      541 cggaattaaa aacgatatcc tgctgagaac ataaacaccc acggatcgag gcgtacgcta
      601 tcgtgttgct gtgcaccgcc tagcttataa ttggcggtgg t
//
LOCUS       DAJTUN010000321          640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTUN010000321 DAJTUN010000000
VERSION     DAJTUN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.327"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>640
                     /locus_tag="OO470_004090"
     CDS             <1..>640
                     /locus_tag="OO470_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR6253136.1"
                     /translation="RCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLD
                     IMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGV
                     FWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELA
                     EQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKL"
BASE COUNT          151 a          187 c          176 g          126 t
ORIGIN      
        1 cgttgccttg ccgaatcagg tactgtaacg ctacgcaagc gccgtcttcc cctcgaaatg
       61 atggtctggt gtattgttgg catggcgctt gagcgtaaag aacctcttca ccagattgtg
      121 aatcgcctgg acatcatgct gccgggcaat cgccccttcg ttgcccccag tgccgttatt
      181 caggcccgcc agcgcctggg aagtgaggct gtccgccgcg tgttcacgaa aacagcgcag
      241 ctctggcata acgccacgcc gcatccgcac tggtgcggcc tgaccctgct ggccatcgat
      301 ggtgtgttct ggcgcacacc ggatacacca gagaacgatg cagccttccc ccgccagaca
      361 catgccggga acccggcgct ctacccgcag gtcaaaatgg tctgccagat ggaactgacc
      421 agccatctgc tgacggctgc agccttcggc acgatgaaga acagcgaaaa tgagcttgct
      481 gagcaactta tagaacaaac cggcgataac actctgacgt taatggataa aggttattac
      541 tcactgggac tgttaaatgg ctggagcctg gcgggagaac accgccactg gatgatacct
      601 ctcagaaagg gagcgcaata tgaagagctc agaaaactgg
//
LOCUS       DAJTUN010000322          638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTUN010000322 DAJTUN010000000
VERSION     DAJTUN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.328"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>638
                     /gene="ynaI"
                     /locus_tag="OO470_004091"
     CDS             <1..>638
                     /gene="ynaI"
                     /locus_tag="OO470_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6253137.1"
                     /translation="LLINQFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFG
                     MSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVA
                     EIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIV
                     EAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQ"
BASE COUNT          162 a          140 c          153 g          183 t
ORIGIN      
        1 ctgctgatta atcagtttga aaaacagcag attaaaaaag gtcgcgatat caccagtgcg
       61 cggattatgt cgcgcattat caaaatcacc attattgtag tgcttgttct gctttatggc
      121 gaacatttcg gcatgagcct ttctggcttg ctgacctttg gtggtattgg tggtctggct
      181 gtcggtatgg ccggtaaaga tattctgagt aatttctttt ccgggatcat gctctatttc
      241 gaccgtcctt tcagtattgg cgactggatc cgttcaccgg acagaaatat cgaaggtaca
      301 gtagcggaaa ttggctggcg aattaccaaa attacgacct ttgataatcg tccattgtac
      361 gtaccgaact cgctgttttc gtcgatcagc gtagaaaacc caggacgaat gaccaaccgc
      421 cgcattacca cgaccattgg tttacgttat gaggatgcgg caaaagtggg cgttattgtc
      481 gaagccgtac gtgagatgct gaaaaatcac ccggccatcg accagcgaca aaccttactg
      541 gtttatttca accagtttgc tgactcgtca ttgaatatta tggtttattg ctttaccaaa
      601 accacggtat gggctgagtg gcttgctgca cagcaaga
//
LOCUS       DAJTUN010000323          637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTUN010000323 DAJTUN010000000
VERSION     DAJTUN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.329"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..567)
                     /gene="baeR"
                     /locus_tag="OO470_004092"
     CDS             complement(<1..567)
                     /gene="baeR"
                     /locus_tag="OO470_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6253138.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHL"
BASE COUNT          149 a          174 c          163 g          151 t
ORIGIN      
        1 aagatgattg agcaattgct cgcgggagaa cacttttcct ggttcgtgag agagcgtttt
       61 aagcagacga aattccgcag gcgtcaggtc aagcatttta ccgcgccatg aagcctgaaa
      121 acgaccttcg tcgataatca acgggctttc ggcatcctgt tgctgaagtt cgcgttgcgg
      181 tttacaacgg cgcaaaatgg ttttgacacg cgctaccact tcccgtgggc tgtacggctt
      241 gcagatgtag tcatcggcgc caatctccag ccccagcagg cggtcgatct cttcgatttt
      301 tgccgtcacc atcacgatcg gaacatcgga aaaacgacga atttcccggc ataatgtcag
      361 gccatcggtg ccagggagca tcagatccaa caggatcaga tccggtggtg tctggcgcac
      421 atacgccagt acctgatcgc cgtggctgat aagcgtcggc gcatagctcg cagcacgcag
      481 ataatcaatg agcaactgcc ccagcttcgg ttcatcttcc acgatcaaaa tacgcggtgt
      541 gttttcgtcg attggtaact cggtcatact tctctctgta aatcccgttc cagcggtaac
      601 tctactgtaa tgcttacccc gccaaaaggc gaatggg
//
LOCUS       DAJTUN010000324          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTUN010000324 DAJTUN010000000
VERSION     DAJTUN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.330"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>635)
                     /locus_tag="OO470_004093"
     CDS             complement(<1..>635)
                     /locus_tag="OO470_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6253139.1"
                     /translation="PEILLFYRMGDFYELFYDDAKRASQLLDISLTKRGASAGEPIPM
                     AGIPYHAVENYLAKLVNQGESVAICEQIGDPATSKGPVERKVVRIVTPGTISDEALLQ
                     ERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEPADRETMAAELQRSNPAELLYAEDF
                     AEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFGTRDLVGFGVENAPRGLC"
BASE COUNT          133 a          196 c          173 g          133 t
ORIGIN      
        1 acaaagtccg cgcggcgcgt tctcgacgcc aaaaccgacc agatcgcggg tgccaaattg
       61 cagattcaac tgctggcgcg cggtgtcgat ttcaaactcc cacagcgggc gacggcgcag
      121 gccgcgacgg ccttcaatca gcgacatctc ggcgaaatct tctgcataca gcagttccgc
      181 cggattagag cgttgcagct ctgccgccat cgtttcgcgg tcggccggtt cgctcaggcg
      241 aaaacgcccg gagctgatat ccagcgtcgc gtagccgaaa cctttgctgt cctgccagat
      301 agccgccagc aggttgtcct gacgctcctg taacagggct tcatcgctga tggtgcctgg
      361 cgtaacgata cgcacaactt tgcgctcaac cggacctttg ctggtcgccg gatcgccaat
      421 ttgttcgcag atggcaacgg actctccctg attcaccagt ttggcgagat agttttccac
      481 cgcatggtag ggaatccccg ccatcgggat cggctctccc gccgaagcac cgcgtttggt
      541 cagtgaaata tccagcagtt gcgacgcgcg ttttgcgtcg tcataaaaca gttcataaaa
      601 atcacccatc cggtaaaaca gcaggatctc gggat
//
LOCUS       DAJTUN010000325          631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTUN010000325 DAJTUN010000000
VERSION     DAJTUN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.331"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..631
                     /locus_tag="OO470_004094"
                     /pseudo
     CDS             1..631
                     /locus_tag="OO470_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          158 a          163 c          174 g          136 t
ORIGIN      
        1 aagaacagtc agatgctcag gccgagatcc gccgtctcca gaaagagctg aaacgggtta
       61 ccgacgaacg ggacatatta aaaaaagccg cggcgtactt cgcaaagctg tccgactgag
      121 gtacgccttt atccgtgaca actcctgttg ctggcctgtt cgcctgctct gtcgggtgct
      181 ggatgttcat cccagtggtt tttacgcctg gcttcagcag ccgcattcac aacgccatca
      241 ggcagacctg agactgacag gacagattaa acagttctgg ctggaatcgg gatgcgtcta
      301 tggttatcgc aaaatccatc tggatctgcg tgacagcggg caacagtgcg gagtaaacag
      361 agtctggaga ctgatgaaac gtgtcggaat aaaggctcag gtcggatacc gaagcccgcg
      421 ggcacgtaaa ggcgaggcca gtatcgtgtc acccaacagg ctccagcgac agttcaatcc
      481 ggatgctcct gatgagcgtt gggtaacgga cataacctac atcaggaccc acgaaggctg
      541 gctgtatctt gccgttgttg ttgatctgtt ctcacgcaaa attatcggct ggtccatgca
      601 atcccggatg acaaaggaca ttgtcctgaa c
//
LOCUS       DAJTUN010000326          629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTUN010000326 DAJTUN010000000
VERSION     DAJTUN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.332"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            444..>629
                     /locus_tag="OO470_004095"
     CDS             444..>629
                     /locus_tag="OO470_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6253140.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSL"
BASE COUNT          178 a          110 c          143 g          198 t
ORIGIN      
        1 agaacgtgcc acggctggct gatggatgtt cgatagcgcg agtttgaatg aaaatcagcc
       61 ggagatgatt ttacataatt gctacggaat tattcaatac aggaattgct tgcgtctgca
      121 tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa ctcgaaaaaa atggtaactg
      181 gttgaatgta ttaatatgca atggtacgtg tcagggatta aaagatgaac gtaaatttat
      241 tcaacgcatt aattttaaag ggttttattg tttgttgacg aaaacaggaa tcatgttcgg
      301 tctcttttta tctgttaaaa gccagaagca tttccttcgc tgactttata gtcaaccata
      361 acacacactc tactgtctga gtccagcgtt ttttaacatt cttgttaaga ttatgtgatc
      421 tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc cgtttatcag agaattgcgg
      481 gtcatcaatg gcgacatatc tggctttctg gcgatataca cggttgtctt gagcagttgc
      541 gccgcaaatt atggcattgt cgttttgatc cgtggcgaga tttacttatc tcagtgggag
      601 acgttatcga tcgtgggccg caaagttta
//
LOCUS       DAJTUN010000327          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTUN010000327 DAJTUN010000000
VERSION     DAJTUN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.333"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>626)
                     /locus_tag="OO470_004096"
     CDS             complement(<1..>626)
                     /locus_tag="OO470_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6253141.1"
                     /translation="VDECFGAGTDARSLTGAQLVQVTRRMAALIVEVIDGTLSPLAQA
                     LMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLLATALHDHPRLREMNV
                     QFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIPIDETDLVSAWQQALL
                     QLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNPHPLLVV"
BASE COUNT          146 a          193 c          188 g           99 t
ORIGIN      
        1 accaccagca ggggatgcgg attcggaaaa cgcgcgacga aatcgaccag cgcgttgatg
       61 accgtcagaa ccgcggcgta acgcacaggc agcgaggcgg gaagcgccag cacgtacacg
      121 tcagttttgg gatcaagatc cagctgaagc agcgcctgtt gccaggcact caccagatcc
      181 gtttcatcaa tcgggatcgc caccggcaaa ttgcgcagcg gcagttgtac gccctccagc
      241 cagattgtgc tgccagagag cgaaagggta tgtgcacccg cgccaatcac cgtggagcgt
      301 acggtttgcg ccggaaactg cacattcatc tcacgcaggc gcggatggtc atgcagcgcc
      361 gttgccagaa gtgggccaat atcggcaaaa cagaacgggt tggcgggctg gtggcgataa
      421 cattcgccca cgcctccaga aagcgtaatg atttcgggcg taacacctgc gggcagcaaa
      481 ccggtttgca tcaatgcctg cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc
      541 agcgccgcca tccgccgggt aacctgcacc agctgcgcgc cggtcagcga acgggcgtca
      601 gtgcctgcac cgaagcactc atccac
//
LOCUS       DAJTUN010000328          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTUN010000328 DAJTUN010000000
VERSION     DAJTUN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.334"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>626)
                     /locus_tag="OO470_004097"
     CDS             complement(<1..>626)
                     /locus_tag="OO470_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6253142.1"
                     /translation="LNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLCV
                     KHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATIL
                     TLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTDA
                     KGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQN"
BASE COUNT          145 a          154 c          169 g          158 t
ORIGIN      
        1 tattctggcg cagttcggcc tccatgcggt cgaactcagc aatgtaggct tccttgaatg
       61 cggcggcttt tttgccagtg aagcccatca ccaggaaaac gaagccgttt ttggtgattt
      121 ggtacattgg gcgtttttcg cctttggcgt cggtataggt gacgggctta aaattaagcc
      181 ggttaaactc cggcgagcat tcgagagttt cgatcttctg aataacgttt ttgtgcattt
      241 tccggaagaa ctccgcaacc gcaacagacg tagtgacagc gcgaccattt tcgatggttg
      301 cgtcagggtg agaaagggta aggatagtag ccatgatggc agcctcgata gtcagtttaa
      361 atgactcacc accagaggta gcaatctcat gggtggtgag acgcacaggg ttgctacaac
      421 cggtgactac cgaaaccggc cagccttgcg gctgccctgc acgccccacc ataatctgaa
      481 tgtggctgtg cttgacgcat aaaaaaaccg cctgagcgcg gttatgcgca gtagtcatcg
      541 tcggggtagc aatcccggca cccgttttat gaggtgcagg tgcactataa ttccacccgt
      601 tctggttttc aatagctaca ttcaac
//
LOCUS       DAJTUN010000329          624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTUN010000329 DAJTUN010000000
VERSION     DAJTUN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.335"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(49..369)
                     /gene="psiF"
                     /locus_tag="OO470_004098"
     CDS             complement(49..369)
                     /gene="psiF"
                     /locus_tag="OO470_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6253143.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(488..>624)
                     /locus_tag="OO470_004099"
     CDS             complement(488..>624)
                     /locus_tag="OO470_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6253144.1"
                     /translation="EEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
                     "
BASE COUNT          146 a          163 c          147 g          168 t
ORIGIN      
        1 tcacagataa aacactctcc aggaaacccg gggcggttca atcaggtatc aggcggcttt
       61 cttgaggcag gcactcataa acttattacg atcatcacct ttcagagctt gttgcgttgc
      121 ttgattatta cattcacgca tcttttgctg ctgtggcgtc aaactttttt caccaggcgc
      181 agacttgctg ttcttcaggc aatcactcat gtaggtctta cgagcatccc ctttcaacgc
      241 ctgcgccgtc gcctgctgat tacaggaggt catacgctgt tgttgtgggg ttaaggttct
      301 ctcggcagcg ccgacggtgg ttaaaaaaac cagaccaaaa agcaaggtaa ccagtaatgt
      361 tattttcata gcaccatccc tcttcatgtt ttaaccatga gcgtttgcgc ccgtgatctg
      421 ccattaagtc tggttgctaa cagcaaaaaa accacccggc agcgaaaatt cactgccggg
      481 cgcagtttta tttcagcccc agagcggctt tcatggtgta gaagagatcg gtctggtcgg
      541 tcagtccaac aacattggcg gcatgcgggc catacgccgc aatacgcaac tgactgccgg
      601 tatgttcttg tgaatcctct tcgg
//
LOCUS       DAJTUN010000330          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTUN010000330 DAJTUN010000000
VERSION     DAJTUN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.336"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(74..553)
                     /gene="folK"
                     /locus_tag="OO470_004100"
     CDS             complement(74..553)
                     /gene="folK"
                     /locus_tag="OO470_004100"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6253145.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
BASE COUNT          157 a          146 c          161 g          159 t
ORIGIN      
        1 aactaaaatt tttattaagg aaagagagga tgtatgattt tcagaaaata tttatattcg
       61 taatataaat aaattaccat ttgcttaatt tgtcaaatgc tcttgtatgt aagacttcac
      121 gcaacgtctc cccatcagga aacgccagct ccggtgcaat ctcaaacagc ggccacagca
      181 taaatccacg attcttcata tcgtagtgcg gaacggtcag gcgttcggta tttatcactt
      241 cattaccaaa cagcatgata tcgagatcca gcgtgcgtgg tccccagcgt tcagctttac
      301 gaacacgacc ctgttgcagt tctatgcgct gtgtatggtt gagtaactct tccggggcgg
      361 gcgaagtttc aagcgccacg gcggcgttga ggtaatcggg ttgatcttgc ggccccagcg
      421 gtggggtgcg gtaaaacgaa gaaacggcaa gaatgcggct ttcagggata tcgcctaatg
      481 ctttcagggc agcattgacc tgctccagcg gagaggccag attgctgcct atggcaatat
      541 acgccactgt catgtggtac cctcacgacg cggtgcgcgt ttgcgtggac gacgagtacg
      601 acgacgcggt gacggttctt cat
//
LOCUS       DAJTUN010000331          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTUN010000331 DAJTUN010000000
VERSION     DAJTUN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.337"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..463)
                     /locus_tag="OO470_004101"
     CDS             complement(<1..463)
                     /locus_tag="OO470_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6253146.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIE"
BASE COUNT          166 a          135 c          139 g          183 t
ORIGIN      
        1 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
       61 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      121 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      181 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      241 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      301 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      361 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      421 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      481 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      541 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      601 cgttaactcg aggcttctta ata
//
LOCUS       DAJTUN010000332          619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTUN010000332 DAJTUN010000000
VERSION     DAJTUN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.338"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..418)
                     /locus_tag="OO470_004102"
     CDS             complement(<1..418)
                     /locus_tag="OO470_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6253147.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQ"
     gene            complement(415..>619)
                     /gene="ssuC"
                     /locus_tag="OO470_004103"
     CDS             complement(415..>619)
                     /gene="ssuC"
                     /locus_tag="OO470_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="part of the ABC type transport system for
                     alkanesulfonate SsuABC; SsuB the ATP-binding subunit and
                     SsuC the permease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6253148.1"
                     /translation="IVAETISANSGIGYLAMNAREFLQTDVVVVAIILYALLGKLADV
                     SAQLLERLWLRWNPAYHLKEATI"
BASE COUNT          145 a          192 c          151 g          131 t
ORIGIN      
        1 gctgtttctg cccgccagaa agtgcggcag gccattcccc ggcgcgattc tccagtcctg
       61 ccgcagccaa cgcccgacgc gcggcatcgc gccactgacc tttaaggcct aacccaacgt
      121 tatcaatcac cgatttccac ggcagcagac gcgcatcctg aaacatcatt cgcgtatcat
      181 cctgaatttc agccagcggt gtggtgcccg ctaacacatc gcctgcggtt ggcgtttcca
      241 gaccggccag caggtgcagc agggtacttt taccaccacc gctgcggccc accaccgcca
      301 caaactgacc tgccggaata tgtaaatcca gttggttcag gacgatattt tccgcgtaat
      361 gtttgcttac tgcattgagc aacaatggtg tgccctggtt cagacgagca gtattcatat
      421 cgtggcctcc ttcaaatgat aagccgggtt ccagcgcaac cagaggcgct ctaacagctg
      481 cgcgctgacg tcagcaagtt tgccgagcag ggcgtaaaga ataatagcga ctaccaccac
      541 gtctgtttgc aaaaactctc gcgcattcat cgccagataa ccaatgccgg aattggcaga
      601 aatggtttcg gcaacaatc
//
LOCUS       DAJTUN010000333          607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTUN010000333 DAJTUN010000000
VERSION     DAJTUN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.339"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            144..467
                     /locus_tag="OO470_004104"
     CDS             144..467
                     /locus_tag="OO470_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6253149.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            464..>607
                     /locus_tag="OO470_004105"
     CDS             464..>607
                     /locus_tag="OO470_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCR6253150.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFD"
BASE COUNT          162 a          136 c          166 g          143 t
ORIGIN      
        1 ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat taagctgatg
       61 aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt gtgcccgcgt
      121 aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa tctcatctcc
      181 ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca caggcggtac
      241 agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag acgattccgg
      301 aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg ctgatgctgg
      361 tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg gaaacgccaa
      421 tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa tcaggcaaag
      481 tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc ggataaagat
      541 ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt atccggagtc
      601 gtttgat
//
LOCUS       DAJTUN010000334          603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTUN010000334 DAJTUN010000000
VERSION     DAJTUN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.340"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..281
                     /locus_tag="OO470_004106"
     CDS             <1..281
                     /locus_tag="OO470_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6253151.1"
                     /translation="TGSTDKLLAVADWRQSPLFSDEERLALEYAEAASVTPPTVDDAL
                     RTRLAAHFDAQALTELTALIGLQNLSARFNSAMDIPAQGLCRIPEKRS"
     gene            291..>603
                     /locus_tag="OO470_004107"
     CDS             291..>603
                     /locus_tag="OO470_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6253152.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NF"
BASE COUNT          136 a          159 c          180 g          128 t
ORIGIN      
        1 gtaccggcag caccgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta
       61 gcgatgaaga acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag
      121 tcgatgatgc cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac
      181 tgacggcatt gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc
      241 ccgctcaggg gctgtgccgt attccggaaa aacgttctta aggagggaag atgcgccatt
      301 gtgggtggtt gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc
      361 aggaaattaa aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata
      421 ccgcaattaa ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa
      481 acctgaagat tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag
      541 ctgctgccgg tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact
      601 tcc
//
LOCUS       DAJTUN010000335          603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTUN010000335 DAJTUN010000000
VERSION     DAJTUN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.341"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(40..180)
                     /locus_tag="OO470_004108"
     CDS             complement(40..180)
                     /locus_tag="OO470_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253153.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            319..>603
                     /locus_tag="OO470_004109"
     CDS             319..>603
                     /locus_tag="OO470_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HCR6253154.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGE"
BASE COUNT          173 a          108 c          137 g          185 t
ORIGIN      
        1 gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga taaataaatt
       61 gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag aaaaaataaa
      121 tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt cttctgacat
      181 aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta aatctgcatg
      241 taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt aacaattatc
      301 cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac cctgcaaggt
      361 gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg cccggttggc
      421 tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga agtctccctt
      481 ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag cagtgaagac
      541 ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat tacgggtggt
      601 gag
//
LOCUS       DAJTUN010000336          603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTUN010000336 DAJTUN010000000
VERSION     DAJTUN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.342"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..231
                     /locus_tag="OO470_004110"
     CDS             <1..231
                     /locus_tag="OO470_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6253155.1"
                     /translation="LKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGS
                     NQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(269..>603)
                     /gene="nhaR"
                     /locus_tag="OO470_004111"
     CDS             complement(269..>603)
                     /gene="nhaR"
                     /locus_tag="OO470_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Na+/H+ antiporter regulatory protein; activates the
                     genes nhaA and osmC; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6253156.1"
                     /translation="RRLLIPGRRSMLGRKLLNWFNSQGLNVEILGEFDDAALMKAFGA
                     MHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERMIQHPAVQRICNTDYS
                     ALFSPAVR"
BASE COUNT          186 a          143 c          125 g          149 t
ORIGIN      
        1 ttaaaaaatg agttagaaaa cagtgaggac ggtgcgggtc aaataggctt tcaactctcc
       61 aatgatggta cgaatgaaat ccagtatggt aaaagcaatt actactcttt tcagcatccc
      121 catgaaggaa gtaatcagat tcctctattt attcgcccgc gtacatatgg aaacaatgtc
      181 tcaagcgggc aaataatgag ccgagtcaaa atcgtggtaa tgtacaacta atctatctcc
      241 cccaccttaa ctttggggga gctgccgatt aacgcaccgc tggactaaaa agcgcagaat
      301 aatccgtatt gcagattcgc tgtaccgccg ggtgctgaat catccgctca gcaaaaatag
      361 catggtactc ttccatcaca ttctcgacgc gaccaatttc tacgacagtt ttatcggcat
      421 aaaagtcata tgcataaagc gttggggcaa cgaagattgc attgtgcatc gcaccaaaag
      481 ctttcatcaa agcggcatca tcaaactcgc cgaggatttc tacgtttaat ccctgggagt
      541 taaaccagtt aagcaatttg cgccctaaca ttgaacgtcg cccaggaatc aaaagtcgcc
      601 gtt
//
LOCUS       DAJTUN010000337          597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTUN010000337 DAJTUN010000000
VERSION     DAJTUN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.343"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            15..254
                     /gene="relB"
                     /locus_tag="OO470_004112"
     CDS             15..254
                     /gene="relB"
                     /locus_tag="OO470_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6253157.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            254..541
                     /gene="relE"
                     /locus_tag="OO470_004113"
     CDS             254..541
                     /gene="relE"
                     /locus_tag="OO470_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6253158.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          161 a          121 c          160 g          155 t
ORIGIN      
        1 aagaggtgta agacatgggt agcattaacc tgcgtattga cgatgaactt aaagcgcgtt
       61 cttacgccgc gcttgaaaaa atgggtgtaa ctccttctga agcgcttcgt ctcatgctcg
      121 agtatatcgc tgacaatgaa cgcttgccgt tcaaacagac actcctgagt gatgaagatg
      181 ctgaacttgt ggagatagtg aaagaacggc ttcgtaatcc taagccagta cgtgtgacgc
      241 tggatgaact ctgatggcgt attttctgga ttttgacgag cgggcactaa aggaatggcg
      301 aaagctgggc tcgacggtac gtgaacagtt gaaaaagaag ctggttgaag tacttgagtc
      361 accccggatt gaagcaaaca agctccgtgg tatgcctgat tgttacaaga ttaagctccg
      421 gtcttcaggc tatcgccttg tataccaggt tatagacgag aaagttgtcg ttttcgtgat
      481 ttctgttggg aaaagagaac gctcggaagt atatagcgag gcggtcaaac gcattctctg
      541 aaccaaagca tgacatctct gtttcgcacc gaaggtgaca cttctgcttt gcgttga
//
LOCUS       DAJTUN010000338          586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTUN010000338 DAJTUN010000000
VERSION     DAJTUN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.344"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>586
                     /gene="proB"
                     /locus_tag="OO470_004114"
     CDS             <1..>586
                     /gene="proB"
                     /locus_tag="OO470_004114"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6253159.1"
                     /translation="SRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRAL
                     LDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRRN
                     PQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPG
                     VIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPA"
BASE COUNT          126 a          148 c          175 g          137 t
ORIGIN      
        1 agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca cgtcgggcaa
       61 atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc ccgcgacacc
      121 ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct
      181 acggcagaga ttaaggtcgg cgataacgat aacctttctg cgctggcggc gattctggcg
      241 ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgatccgcgc
      301 agaaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc actgcgcgcg
      361 attgccggtg acagcgtttc aggcctcgga actggcggca tgagtactaa attgcaggcc
      421 gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc
      481 gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact
      541 ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgg
//
LOCUS       DAJTUN010000339          586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTUN010000339 DAJTUN010000000
VERSION     DAJTUN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.345"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>586
                     /gene="mukB"
                     /locus_tag="OO470_004115"
     CDS             <1..>586
                     /gene="mukB"
                     /locus_tag="OO470_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="SMC (structural maintenance of chromosomes) family
                     of proteins; involved in chromosome condensatin and
                     partitioning; forms a homodimer and the C-terminal is
                     essential for DNA-binding activity while the purified
                     N-terminal domain binds FtsZ; mutations result in cell
                     division defects; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6253160.1"
                     /translation="IELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQ
                     GLQNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQR
                     LNPQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMS
                     ILVMVVQSWEDESRRLRGKDISPCRLLFLDEAARL"
BASE COUNT          137 a          145 c          170 g          134 t
ORIGIN      
        1 gattgaactt agccgtctga ccgaagaatt aacctcccgt gaacagaaac tggcgatcag
       61 ttcccgcagc gtggcgaaca tcattcgcaa aaccattcag cgcgagcaga accgtatccg
      121 tatgctcaac caggggttgc agaacgtatc gtttggtcag gtgaacagcg tgcgtctcaa
      181 cgtgaacgtg cgtgaaacgc acgccatgct gctggatgtg ctctctgaac agcacgagca
      241 gcatcaggat ctgtttaaca gcaaccgtct gaccttctcg gaagcgttgg cgaaattgta
      301 tcagcgtctt aatccgcaga ttgatatggg gcagcgcacg ccgcagacca ttggcgaaga
      361 actgctggat taccgcaact atctggaaat ggaagttgag gttaaccgtg gttccgatgg
      421 ttggttgcgc gcagagtctg gtgcattgtc gaccggtgag gcgattggta ccggtatgtc
      481 gattctggtg atggtggtac aaagctggga agatgaatct cgccgcctgc gcggtaaaga
      541 tatctctcct tgccgcctgc tgttcctcga tgaagcagcg cgactg
//
LOCUS       DAJTUN010000340          583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTUN010000340 DAJTUN010000000
VERSION     DAJTUN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.346"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..583
                     /locus_tag="OO470_004116"
                     /pseudo
     CDS             1..583
                     /locus_tag="OO470_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          149 a          164 c          158 g          112 t
ORIGIN      
        1 ccagtaaaaa accccgtaaa cagcattcgc ctgaatttcg cagtgaagcc ctgaagcttg
       61 ctgaacgcat cggtgttact gccgcagccc gtgaactcag cctgtatgaa tcacagctct
      121 acaactggcg cagtaaacag caaaatcagc agacgtcttc tgaacgtgaa ctggagatgt
      181 ctaccgagat tgcacgtctc aaacgccagc tggcagaacg ggatgaagag ctggctatcc
      241 tccaaaaggc cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      301 acatcaggct gagttcagca tcaaagcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      361 ctggtatacg tggtgtcagc ggcggacaag gataagcacg cgtcagcagt tccgccaaca
      421 ctgcgacagc gttgtcctcg cggcttttac ccggtcaaaa cagcgttacg gtgccccacg
      481 cctgacggat gaactgcgtg ctcagggtta cccctttaac gtaaaaaccg tggcggcaag
      541 cctgcgccgt cagggactga gggcaaaggc ctcccggaag ttc
//
LOCUS       DAJTUN010000341          579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTUN010000341 DAJTUN010000000
VERSION     DAJTUN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.347"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            21..>579
                     /gene="exuR"
                     /locus_tag="OO470_004117"
     CDS             21..>579
                     /gene="exuR"
                     /locus_tag="OO470_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6253161.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSR"
BASE COUNT          157 a          144 c          150 g          128 t
ORIGIN      
        1 ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat caacaacttg
       61 ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat aaactgcctg
      121 ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt gaagccatca
      181 tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt catgtggttt
      241 ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc aattacggtc
      301 cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag ttcgcggcaa
      361 ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag gcgcgcggcg
      421 aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta gccctggcga
      481 cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt agccataacc
      541 cgtactggaa aaaactacac gaacacattg attcccgta
//
LOCUS       DAJTUN010000342          578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTUN010000342 DAJTUN010000000
VERSION     DAJTUN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.348"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>578
                     /locus_tag="OO470_004118"
     CDS             <1..>578
                     /locus_tag="OO470_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6253162.1"
                     /translation="ARAASTSAGSAASSASSASASKDEATKQASAAKGSATTASSKAT
                     ESAGSATAAAQSKSAAESAAMRAETAAKRAEDIADVVSLEDASTTKKGIVQLSSATNS
                     ASESLAATAKAVKVVMDETNKKAPLNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAI
                     AALVDSSPDALNTLNELAAALGNDPNFATTMT"
BASE COUNT          154 a          162 c          175 g           87 t
ORIGIN      
        1 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
       61 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      121 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      181 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      241 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      301 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      361 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      421 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      481 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      541 gctgggcaac gacccgaatt ttgcgaccac catgacta
//
LOCUS       DAJTUN010000343          575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTUN010000343 DAJTUN010000000
VERSION     DAJTUN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.349"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..429
                     /gene="csgB"
                     /locus_tag="OO470_004119"
     CDS             <1..429
                     /gene="csgB"
                     /locus_tag="OO470_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6253163.1"
                     /translation="LTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAIIGQAG
                     TNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQGAYGN
                     TAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            470..>575
                     /locus_tag="OO470_004120"
     CDS             470..>575
                     /locus_tag="OO470_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6253164.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT          168 a          108 c          149 g          150 t
ORIGIN      
        1 ttaacaatac tgggtgcgcc tgggattgca gccgcagcag gttatgattt agctaattca
       61 gaatataact tcgcggtaaa tgaattgagt aagtcttcat ttaatcaggc agccataatt
      121 ggtcaagctg ggactaataa tagtgctcag ttacggcagg gaggctcaaa acttttggcg
      181 gttgttgcgc aagaaggtag tagcaatcgg gcaaagattg accagacagg agattataac
      241 cttgcatata ttgatcaggc gggcagtgcc aacgatgcca gtatttcgca aggtgcttat
      301 ggtaatactg cgatgattat ccagaaaggt tctggtaata aagcaaatat tacacagtat
      361 ggtactcaaa aaacggcaat tgtagtgcag agacagtcgc aaatggcaat tcgcgtgaca
      421 caacgttaat taccattcga cttttaaatc aatccgatgg gggttttaca tgaaactttt
      481 aaaagtagca gcaattgcag caatcgtatt ctccggtagc gctctggcag gtgttgttcc
      541 tcagtacggc ggcggcggta accacggtgg tggcg
//
LOCUS       DAJTUN010000344          563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTUN010000344 DAJTUN010000000
VERSION     DAJTUN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.350"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..482
                     /locus_tag="OO470_004121"
     CDS             <1..482
                     /locus_tag="OO470_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6253165.1"
                     /translation="QFWHIPHEKIWIEDQSTNCGENARFSITLLNQAVERVHTAIVVQ
                     DPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYL
                     SLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT          140 a          140 c          133 g          150 t
ORIGIN      
        1 atcagttctg gcatattccg catgaaaaaa tctggattga agaccagtca acaaactgcg
       61 gtgaaaacgc acgctttagc atcacgctat tgaatcaggc cgtagaacga gttcatacgg
      121 ctatcgttgt acaggacccc accatgcagc ggcgcacgat ggcaacgttc cgccgtatga
      181 ccggggacaa tcccgatgca ccacgctggt taagttatcc cggattcgtt cctcagttag
      241 gaaataacgc agacagtgta atctttatta atcagttaca aggattatgg ccggttgagc
      301 gttatctctc actactcact ggcgagctgc cgcgtttaca cgatgatagc gatggctacg
      361 gtccccgcgg gcgagatttt atcgttcacg ttgattttcc ggcagaagtc atccatgcat
      421 ggcaaacgct gaaacatgat gcggtgctca tcgaggcgat ggaaagtcgc tcgttacgtt
      481 aaaaattgcc cgtttgtgaa ccacttgttt gcaaacgggc atgactcctg acttttattt
      541 ctgcctttta ttccttttac act
//
LOCUS       DAJTUN010000345          561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTUN010000345 DAJTUN010000000
VERSION     DAJTUN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.351"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..465
                     /gene="cobU"
                     /locus_tag="OO470_004122"
     CDS             <1..465
                     /gene="cobU"
                     /locus_tag="OO470_004122"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6253166.1"
                     /translation="LYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELIHAD
                     INPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAK
                     VVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
     gene            462..>561
                     /gene="cobS"
                     /locus_tag="OO470_004123"
     CDS             462..>561
                     /gene="cobS"
                     /locus_tag="OO470_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="catalyzes the formation of adenosylcobalamin from
                     Ado-cobinamide-GDP and alpha-ribazole; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6253167.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRG"
BASE COUNT          135 a          115 c          163 g          148 t
ORIGIN      
        1 ctgtatatcg ctacctcgca aatccttgat gatgagatgg ctgcacggat agaacatcat
       61 cggcaaagcc gcccggagca ctggcgcaca gtggagcgct ggcaacatct tgatgaatta
      121 attcatgcag acattaaccc gaatgaggct gtgttgctgg aatgcgttac cacaatggtg
      181 actaatctgt tgtttgatta tggcggcgat aaagaccctg atgaatggga ttatcaggcg
      241 atggaacagg cgattaatgc tgagattcag tcgttgattg ctgcctgcca acgttgcccc
      301 gcaaaggttg tattagtgac taacgaagtg ggaatgggga ttgtgccgga gagtcgtctg
      361 gcacgacatt ttcgtgatat tgccgggcgg gtaaatcagc agttggccgc tgcggcaaat
      421 gaagtatggc tggtggtttc gggtattgga gtaaaaatca aatgagcaaa ttattttggg
      481 cgatgctctc atttattacg cgtctgcctg taccgcgtcg ctggtcccag ggactggatt
      541 tcgagcatta ttctcgcggt a
//
LOCUS       DAJTUN010000346          559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTUN010000346 DAJTUN010000000
VERSION     DAJTUN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.352"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>559)
                     /locus_tag="OO470_004124"
     CDS             complement(<1..>559)
                     /locus_tag="OO470_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6253168.1"
                     /translation="LSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLK
                     QFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCGAM
                     YLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPG
                     SFLPVAHEFGLSSSIDMWVIEHTLQF"
BASE COUNT          146 a          147 c          134 g          132 t
ORIGIN      
        1 aaattgcagc gtatgctcaa tgacccacat gtcgatactc gacgataaac caaattcgtg
       61 cgcgaccggc aagaagctac cggggccgat cagttcatca ttctcacctt tcatgcgcag
      121 aagaatttca tggtaaacat caccacgcat accggtaatc ggctgggcca tcaggaaaaa
      181 atggttgtgt tccagcgcct gctgtagtcg attcatcatc gcgactttat ctttcaattc
      241 gcgttgcaaa tacattgccc cgcaacgctg catattttcc ggggcgttgg tcacgatgga
      301 aagttcggcg accgtattta gctctcccag cagcaggtag atatgattca ctggcgagcg
      361 cacatagcag taactgacgc caatctgcgg ttgcatcggc atcccatccc aaaagaaacg
      421 aaattgcttg agatgactat ccagtgcggt aatgcgctcc tggtgcgatt ctgtattcag
      481 tcgcagcgcg agatcgttac ccgaaagctg ataaacatct tcacccggtt ccagcaaggg
      541 tgacagccag tgagaaagt
//
LOCUS       DAJTUN010000347          556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTUN010000347 DAJTUN010000000
VERSION     DAJTUN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.353"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            94..>556
                     /gene="nanR"
                     /locus_tag="OO470_004125"
     CDS             94..>556
                     /gene="nanR"
                     /locus_tag="OO470_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6253169.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKA"
BASE COUNT          141 a          141 c          138 g          136 t
ORIGIN      
        1 atgaggcaca aagagaaaaa cctgccattt tcccctactt tcaatcctgt gataggatgt
       61 cactgatgat gttaatcaca ctgaccttac agaatgggcc ttatgaacgc atttgattcg
      121 caaaccgaag attcttcacc tgcaattggt cgcaacttgc gtagccgccc gctggcgcgt
      181 aaaaaactct ccgaaatggt ggaagaagag ctggaacaga tgatccgccg tcgtgaattt
      241 ggcgaaggtg aacaattacc gtctgaacgc gaactgatgg cgttctttaa cgtcgggcgt
      301 ccttctgtgc gtgaagcgct ggcagcgtta aaacgcaaag gtctggtgca aataaacaac
      361 ggcgaacgcg ctcgtgtctc tcgaccttct gcggacacta tcatcggcga gctttccggc
      421 atggcgaaag atttcctttc tcatcctggt gggattgccc atttcgaaca attacgtctg
      481 ttctttgaat ccagcctggt gcgctatgcg gctgaacacg ccaccgatga gcaaatcgat
      541 ttgctggcaa aagcac
//
LOCUS       DAJTUN010000348          555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTUN010000348 DAJTUN010000000
VERSION     DAJTUN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.354"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(38..555)
                     /locus_tag="OO470_004126"
                     /pseudo
     CDS             complement(38..555)
                     /locus_tag="OO470_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
BASE COUNT          183 a          138 c          125 g          109 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaata tggcagtgct attttgattc
       61 gacgttgatc aagtttctga taattccaac gacggtcgac gggcaccatc cagtcaatca
      121 acatacctac cgccagccct gtgatgacat caataaaatg atgctgccat gtcgtcagcg
      181 tcgaaatggc gatgagtaaa aaccatccgc cacagacttt acgccacctc acagccagat
      241 gctgacgaaa gtgacgccaa agtagccagc agagaataat atgcagcgaa ggagactggt
      301 tataaggcag atcaaacagt tcaagttgcg aaaatagcca tcctgtcacc ccactcactt
      361 caggacggat aaaactaaac ttcagcgggt agagaaaaaa accgcagcag gccattaccg
      421 ttgccagaat aagccggtgg acaagtcggc gttgttcgaa tgtcgagcta caaacgaaca
      481 gcgaaaatcc atataaaaga tccagactcc agtaaggaac aatagtccag ggaagaaaag
      541 ggatcgccgt ttccc
//
LOCUS       DAJTUN010000349          555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTUN010000349 DAJTUN010000000
VERSION     DAJTUN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.355"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>555)
                     /locus_tag="OO470_004127"
     CDS             complement(<1..>555)
                     /locus_tag="OO470_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460876.1"
                     /note="serine sensor receptor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6253170.1"
                     /translation="MGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLS
                     SRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKT
                     MHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLAS
                     RSAQAAKEIKALIEDSVSRIDTGS"
BASE COUNT          105 a          161 c          159 g          130 t
ORIGIN      
        1 ccgaaccggt atcaatgcgc gagacggagt cttcaatgag ggctttgatc tcttttgccg
       61 cctgggcgct gcgactggca agattacgca cttcacccgc caccacggca aaaccacggc
      121 cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata ttagtctgga
      181 aggcaatacc gtcgataacg ctgataatgt cggcaatttt cttcgaacta tcggcgatct
      241 catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg gtgtcggagg
      301 cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc actgtcgcgg
      361 tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt tcagtacggg
      421 aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg gcatctgaac
      481 cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa acgctctgcg
      541 ccaggtcacc cattt
//
LOCUS       DAJTUN010000350          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTUN010000350 DAJTUN010000000
VERSION     DAJTUN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.356"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            22..483
                     /locus_tag="OO470_004128"
     CDS             22..483
                     /locus_tag="OO470_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6253171.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT          167 a          119 c          121 g          143 t
ORIGIN      
        1 ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct cgttgttgca
       61 tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa gaaattcagc
      121 gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga caaggtgctt
      181 aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac caaagaagat
      241 gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg tgttgaagaa
      301 aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat ggaaaaagtg
      361 gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga tgccaaaaaa
      421 ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa agaagtgaaa
      481 taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt ttcttcacat
      541 gctgacgcgc
//
LOCUS       DAJTUN010000351          547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTUN010000351 DAJTUN010000000
VERSION     DAJTUN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.357"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..349)
                     /locus_tag="OO470_004129"
     CDS             complement(<1..349)
                     /locus_tag="OO470_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6253172.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAP"
BASE COUNT          142 a          146 c          119 g          140 t
ORIGIN      
        1 ctggcgcacg atatttcggg tagcgcagac ggttttcact gttgacgtaa tccagcaaac
       61 cggccccttt cgggcacagc gcaccacggc ttaccggatg atccgggtca ccttcaatgt
      121 gataaatcgc ttctctggcg ttttttgcgc catcacccag gctatacatc aatagcccgc
      181 aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat aatttgtagt
      241 ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc gttgttccag
      301 ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt gctttcctct
      361 tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc tttttgcgcg
      421 aaatcaaaaa gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt gacctacctc
      481 aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg ctataaaatt
      541 cagctta
//
LOCUS       DAJTUN010000352          546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTUN010000352 DAJTUN010000000
VERSION     DAJTUN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.358"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            298..534
                     /locus_tag="OO470_004130"
     CDS             298..534
                     /locus_tag="OO470_004130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253173.1"
                     /translation="MSALKETASMLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSL
                     IDELTISKSNEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          171 a          116 c          121 g          138 t
ORIGIN      
        1 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
       61 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
      121 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
      181 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
      241 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
      301 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
      361 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
      421 ttgtctctca ttgatgagct gacaatctct aaaagcaatg aggctgcgct caaagcaaaa
      481 ctcacggaga tagaaaagtt taagttccaa agtgaagact gcgaacctcg ctaattgatt
      541 actgga
//
LOCUS       DAJTUN010000353          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTUN010000353 DAJTUN010000000
VERSION     DAJTUN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.359"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>544
                     /locus_tag="OO470_004131"
     CDS             <1..>544
                     /locus_tag="OO470_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6253174.1"
                     /translation="KMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEF
                     ALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNH
                     HRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWL
                     VQAGVPLSVLQEMGGWESIE"
BASE COUNT          170 a          112 c          150 g          112 t
ORIGIN      
        1 ggaaaatgct ggataaggca ccaattatta aagtgcctca accaaagaat aaacggatcc
       61 gctggctgga gccccatgaa gcacaaaggc tgattgatga atgtccggag ccattaaagt
      121 ctgttgttga atttgcactg gcaacaggct taagacgctc gaacatcatc aaccttgaat
      181 ggcaacaaat agatatgcag cgccgggtgg catggataaa cccggaagag agtaaatcaa
      241 accgcgcaat tggcgttgcg ctgaatgata ctgcatgtcg cgtattgaaa aaacaaatcg
      301 ggaatcatca ccgttgggta tttgtgtaca aggaaagctg taccaaacca gacggaacga
      361 aagcgccaac agtaaggaag atgcggtatg acgcaaacac agcctggaaa gcggcgctga
      421 gacgggctgg tattgatgat ttcagatttc acgacttgag acacacctgg gcaagttggc
      481 tggttcaagc cggagtcccg ttgtcagtgt tacaggaaat gggaggctgg gagtctatcg
      541 aaat
//
LOCUS       DAJTUN010000354          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTUN010000354 DAJTUN010000000
VERSION     DAJTUN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.360"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT          152 a          113 c          131 g          148 t
ORIGIN      
        1 tctgcccttt aataatctac ggacagatta ctttctgtac ttccagagcc tgtttctgtg
       61 agtcaaacat gagctggttt ttaccgacta cacccaagta aatatattcg atatctccca
      121 tttgccgtat tttcgtaaaa ttcaacagga aatattcaac gggagtgaga gaactgctgt
      181 ccggggagga ggtacagacg caaaaaaacc gactggtgag gccggttttt ttgcgattcc
      241 ggatcgaagt tggtagccct gaccggaaga taactgccta taactgacta tgaaataata
      301 cggctgaaat aatatttctg caagcacagt ataagtatat actcggtgtg tcagctttga
      361 ggcacgttct ttccggcata tcgttcaccg ggatgaacag gttgcaagtc ctggccaaaa
      421 catcaggtgg tgctggtttt ggtgctctgg ccggagaaga actgtctata actgacacta
      481 tagaaaacgt ggcgacaggc aatgtaaggg caaaccacgg tgttctttct ccattgatgg
      541 agaa
//
LOCUS       DAJTUN010000355          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTUN010000355 DAJTUN010000000
VERSION     DAJTUN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.361"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>541
                     /gene="rlmB"
                     /locus_tag="OO470_004132"
     CDS             <1..>541
                     /gene="rlmB"
                     /locus_tag="OO470_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6253175.1"
                     /translation="LIHALESQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQE
                     NDLPDLIASLDQPFLLILDGVTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKK
                     VACGAAESVPLIRVTNLARTMRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAE
                     GEGMRRLTREHCDELISIPM"
BASE COUNT          121 a          150 c          165 g          105 t
ORIGIN      
        1 gctgattcac gcccttgagt ctcagggcgt ggttatccag ttggcaaacc gccaatatct
       61 cgacgagaaa agcgacggtg ccgtgcatca gggcattatc gcccgcgtga agccaggacg
      121 tcagtatcag gaaaacgatc tgccggatct gatcgcttcg ctcgatcaac cgttcctgct
      181 gatcctcgac ggcgtaaccg atccgcacaa cctcggcgcg tgcctgcgta gtgcggacgc
      241 cgcaggcgtt catgcggtga ttgtgccgaa agatcgctcc gcacagctca acgctacggc
      301 gaaaaaagta gcctgcggcg cggcagaaag cgttccgctg attcgggtga ctaaccttgc
      361 gcgcaccatg cgtatgttgc aggaagagaa tatctggatc gtcggtacgg caggcgaggc
      421 ggatcataca ctctatcaga gcaaaatgac cggacgcctg gcgctggtga tgggcgcgga
      481 aggtgaaggt atgcgtcgcc tgacccgtga acattgcgat gagttgatca gcatcccaat
      541 g
//
LOCUS       DAJTUN010000356          527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTUN010000356 DAJTUN010000000
VERSION     DAJTUN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.362"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..477
                     /locus_tag="OO470_004133"
     CDS             <1..477
                     /locus_tag="OO470_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCR6253176.1"
                     /translation="KTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARH
                     NISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIG
                     NDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT          111 a          135 c          142 g          139 t
ORIGIN      
        1 aaaactgaaa atccgccgct gttccgcgcg ctggcgcttc gtcgcaatca gactctgctc
       61 actttgcaca gcctgaatat gctgcattct cgcggcttcc tcgcggaagt tttcggcatc
      121 ctcgcgcggc ataatatttc ggtagactta atcaccacgt cagaagtgag cgtggcatta
      181 acccttgata ccaccggttc aacctccact ggcgatacgt tgctgacgca atctctgctg
      241 atggagcttt ccgcactgtg ccgggtggag gtggaagaag gtctggcgct ggtcgcgttg
      301 attggcaatg acctgtcaaa agcctgcggc gttggcaaag aggtattcgg cgtactggaa
      361 ccgttcaaca ttcgcatgat ttgttacggc gcatccagcc ataacctgtg cttcctggtg
      421 cccggcgaag atgccgagca ggtggtgcaa aaactgcata gtaatttgtt tgagtaaata
      481 ctgtatggcc tggaagctat atttcgggcc gtattgattt tcttgtc
//
LOCUS       DAJTUN010000357          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTUN010000357 DAJTUN010000000
VERSION     DAJTUN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.363"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>524)
                     /gene="malI"
                     /locus_tag="OO470_004134"
     CDS             complement(<1..>524)
                     /gene="malI"
                     /locus_tag="OO470_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6253177.1"
                     /translation="TGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYA
                     ELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRR
                     MAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLT
                     RAERVGGYCATLLK"
BASE COUNT          121 a          171 c          121 g          111 t
ORIGIN      
        1 attttagtag agttgcacaa tagcccccca cccgttctgc acgggttaat gaggaacttt
       61 gccctcccag ccaggcgatc cgctgatgcc cattgcgaat gagatgctcc gtcaacaact
      121 gtgcagcctg catgttgtcc gggcgaaccg tatcaacatc atcaagataa ctggcacggg
      181 aagcgaaaat caccgggata gctttttctt ctgccatccg ccgcaggtca tcgctgcttc
      241 ctgcagcccc ggcaattacc acaccatcga caccctgatt cagtaacagt gaaaaccgct
      301 gtgccagctg ctcgccgtct ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt
      361 ccagagcttc cgtcaatccg gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga
      421 cgatcaaacc aatgacgccg ctttgcccgc cgcgcagcgc cgacgcctgt cgattgcgca
      481 caaatcccag ctcttcaatg gcggcgttaa cgcgttctcc tgtg
//
LOCUS       DAJTUN010000358          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTUN010000358 DAJTUN010000000
VERSION     DAJTUN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.364"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..487
                     /gene="rhaD"
                     /locus_tag="OO470_004135"
     CDS             <1..487
                     /gene="rhaD"
                     /locus_tag="OO470_004135"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6253178.1"
                     /translation="PTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLEND
                     TAVFTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVF
                     GSGPTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALA
                     L"
BASE COUNT          126 a          146 c          141 g          111 t
ORIGIN      
        1 tccaacttct gaacttccgg ctcactttct ctcccactgc gagcgcatta aagccaccaa
       61 cggtaaagat cgggtgatca tgcactgcca cgccaccaac ctgatcgccc tcacctatgt
      121 actggaaaac gacaccgcgg tctttactcg ccaactgtgg gaaggcagca ccgaatgtct
      181 ggtagtattc ccggatggcg ttggcattct gccgtggatg gtgcccggca cggacgaaat
      241 cggccaggcg accgcacaag agatgcaaaa acattcgctg gtgttgtggc ccttccacgg
      301 tgtgttcggt tccggaccga cgctggatga agcctttggt ttaatcgaca ccgcagaaaa
      361 atcagcacaa gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagccg
      421 tgaagagttg atagcgctcg gcaagcgctt cggcgttaca ccactagcca gtgcgctggc
      481 gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg cagg
//
LOCUS       DAJTUN010000359          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTUN010000359 DAJTUN010000000
VERSION     DAJTUN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.365"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            76..255
                     /locus_tag="OO470_004136"
     CDS             76..255
                     /locus_tag="OO470_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253179.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            307..501
                     /locus_tag="OO470_004137"
     CDS             307..501
                     /locus_tag="OO470_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253180.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          175 a          115 c          113 g          110 t
ORIGIN      
        1 tggttgtctg gagattcagg gggccagtct aggtagcgta aggcactaca gcgcattgct
       61 ttacgagtaa taacaatgga tttatcacca gaagacgcaa agtttttacg agaactgcaa
      121 caccgtatgc tcagagaagc cattatcgaa cagcaaacgg gcatgagcgc catagaccca
      181 ttgcgataca aacgggcctc atctccttta gttgacgaca attccaatgc ggataaaaca
      241 cgtccaccac actgattaca tttttcatta gcacagtggt gtgatgcagc atacgaggaa
      301 aaccggatga atgagaacaa aataaaaagg ctggagcaac tgttacaggc acgccaacgg
      361 gagtttgcca ctaaatgggg acgtgccgcg ctgccacatg aacgcctaac ctattttcca
      421 cttgaagacc tcattaaaaa taatggcaaa aaaatcaggt ctgataagaa acacttctta
      481 gaatgcaatg aagacaaata acaacaatct gca
//
LOCUS       DAJTUN010000360          512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTUN010000360 DAJTUN010000000
VERSION     DAJTUN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.366"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(236..>512)
                     /locus_tag="OO470_004138"
     CDS             complement(236..>512)
                     /locus_tag="OO470_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6253181.1"
                     /translation="WGKQFHDFVYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSL
                     ASSPATRSELKVTFDNAPPGMRVIDLPKTGDPFMKITHDVGYSPFKR"
BASE COUNT          146 a          120 c           99 g          147 t
ORIGIN      
        1 cgatatcctc agttgtctga atccatctgt atccaagtca acgtcattat ctgcagatgt
       61 tttaacgtta ctaataaatc gtgaaacaat ctcctcattt gttagtattt tcgcctgagc
      121 acagcaagag gttaatattg caaccgtcat taaaaatggc tttatcacaa aacaaactcc
      181 attgttagca tgttacgcga taattttgcc tcatctttca ccacaaaaaa agaactcacc
      241 ttttaaatgg agaataccca acatcatggg tgattttcat aaagggatcg cctgttttcg
      301 gcaaatcgat aacgcgcatt ccaggcggcg cgttgtcaaa cgtcacctta agttcgctgc
      361 gggttgccgg tgaagatgca aggcttaaca gctgattcct ttccagtgat acattcgggg
      421 tgtagcgttt atcgggagat aaatacccat caccacgtgg catctgccat ccggtcatgt
      481 catagacaaa atcatggaac tgtttgcccc ac
//
LOCUS       DAJTUN010000361          499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTUN010000361 DAJTUN010000000
VERSION     DAJTUN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.367"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..245)
                     /locus_tag="OO470_004139"
     CDS             complement(<1..245)
                     /locus_tag="OO470_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6253182.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFV"
     gene            complement(284..>499)
                     /locus_tag="OO470_004140"
     CDS             complement(284..>499)
                     /locus_tag="OO470_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253183.1"
                     /translation="IVSHCRIRCLQSQQRKSGAVLFYFCNGLLTDGFILREDEFVTSL
                     RVLKKIGFKAGFLLLLKNKLIYNLEQD"
BASE COUNT          148 a          121 c          107 g          123 t
ORIGIN      
        1 tcaacgaaac cggacaccag ttttcgttcg cgaggcgtga tcgccccacc gaccggagtc
       61 cagtaaacga aacccagttg caggagtttg aaaaaacgct tgtggaatgc gtagttacgc
      121 acacgcttaa agtctgcgtg tatccactca cctattttga tttgatgcag aaaatcgcaa
      181 ctctccggcg tcgccgggag aagtaatccg gaagaggttt gtttgaccag ttgtatatgt
      241 gccatcggct ttctccggtg gcacggtgtt acacagcagg agttcaatcc tgctcaagat
      301 tgtagatgag tttattcttc agcaaaagca gaaaaccagc cttaaaacca atctttttca
      361 aaacccgtaa tgatgtgaca aattcgtcct cacgcaaaat aaaaccgtcc gtcagaagtc
      421 cattacaaaa ataaaataac acagcaccgc tcttcctttg ttgagattgc aaacatctaa
      481 tgcggcaatg gctgacaat
//
LOCUS       DAJTUN010000362          498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTUN010000362 DAJTUN010000000
VERSION     DAJTUN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.368"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..208)
                     /locus_tag="OO470_004141"
     CDS             complement(<1..208)
                     /locus_tag="OO470_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6253184.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAAR"
BASE COUNT          158 a          126 c          104 g          110 t
ORIGIN      
        1 cccgtgcagc cttacgcttg tcttctctta ttttgaaata cagattcgtc agataagtca
       61 gaagccccag aagcagactt cccagcacac caatcgcagc ccactgtgat ggcctgacct
      121 gatccaacca ctgcaaaaac cagtagccgg cactgccggc ggaggtgccg taggcaatgc
      181 ccgttgaaat tttgtccatg gatttcatag cctcacctcc gcaaataacg gatggcgtag
      241 ttttacactg agaaatgaaa gggatttgaa aagaaaaaaa cgcaaaagcg ggcgaaacga
      301 tatatacagt aaggaaagca ctctatccaa caaaccaccc acagttaatc ggaataaaag
      361 cagagtgctt atgaatgatc gcacgcccga aggttagtat ttctgcacag caattttgca
      421 aaaaaaggcg atcattcata acttaaacgt ctttcagtca ctccgggatt tcccatcatc
      481 gcagactgaa agactctg
//
LOCUS       DAJTUN010000363          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTUN010000363 DAJTUN010000000
VERSION     DAJTUN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.369"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(171..>494)
                     /locus_tag="OO470_004142"
     CDS             complement(171..>494)
                     /locus_tag="OO470_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="regulates the glyoxylate bypass operon (aceBAK),
                     which encodes isocitrate lyase, malate synthase and
                     isocitrate dehydrogenase kinase/phosphorylase; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6253185.1"
                     /translation="QLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSF
                     DDEEHALGLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLA
                     YGGMR"
BASE COUNT          128 a          117 c          114 g          135 t
ORIGIN      
        1 ttttgttaat aattaagtgg tttgatgttt aaaaataatg ctgagttaat aaaagtgtgt
       61 gaagtgtatg acattaagtt attttggcgt caatgcgatt aacagacacc cttattctat
      121 tgccactcag gtatgatggg cagaatattg cctctgcccg ccagaaaaag tcagcgcatt
      181 ccaccgtacg ccagcgtcac ttccttcgcc gctttaatca ccatcgcgcc aaactcggtc
      241 acgcgatcat cggtaatacg tgaaatcggt ccggaaatag aaattgcggc aaacggttcg
      301 cggtgctcat cgaaaataca cgctgcaagg caacgtagcc ccagcgcatg ttcctcatcg
      361 tcaaatgaat aaccccgttt gcgcgtttgg gcgagatctt cttttaaatg cacaggagac
      421 accagagttg cgtgggtata ggcatgtaac cctttgcggt gcagcagctt cgtcacctgt
      481 tcttcgctca gttg
//
LOCUS       DAJTUN010000364          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTUN010000364 DAJTUN010000000
VERSION     DAJTUN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.370"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>493)
                     /gene="treF"
                     /locus_tag="OO470_004143"
     CDS             complement(<1..>493)
                     /gene="treF"
                     /locus_tag="OO470_004143"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="cytoplasmic; catalyzes the hydrolysis of trehalose
                     to glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR6253186.1"
                     /translation="RSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLI
                     PNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAA
                     SGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQ
                     KASA"
BASE COUNT          108 a          144 c          141 g          100 t
ORIGIN      
        1 gggcactggc cttctggcgg aacagcgctt ctgtctcttt ctcgcctttc agcgccgaga
       61 tgttggcgat ggcgctctcc agtttaaaca ggaaggcatt cagatcgatg gggatgaact
      121 gggtggtacg aatgctcgcc agacgaccag tatcacgcag ccaacgggaa gagtaatccc
      181 aaccggaggc cgctcccgcg cgtaaatcgc ggtacacctc gttgggcggg cgaccagaat
      241 gtttcgcggt ttcaacgtcc tcaagccagg attcgtcacg cggcgtgtcg cgatcgtccc
      301 agtaacggtt gagcagcgat ccgtccggca tccgcacaac atggcgatag gcctgattag
      361 ggatcaacga ttctgcaccg tccatccaga aggcatattc cattttaagg tggtcgagat
      421 agcggcgcgc accgcgtaca ccatcttctt caaacaactc caccgtcagc gcaaaaaccg
      481 gtggttgcga gcg
//
LOCUS       DAJTUN010000365          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTUN010000365 DAJTUN010000000
VERSION     DAJTUN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.371"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..241
                     /locus_tag="OO470_004144"
     CDS             <1..241
                     /locus_tag="OO470_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6253187.1"
                     /translation="AYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIRD
                     LNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            207..>488
                     /locus_tag="OO470_004145"
     CDS             207..>488
                     /locus_tag="OO470_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253188.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGN"
BASE COUNT          122 a          121 c          138 g          107 t
ORIGIN      
        1 cgcttatcag ggctcagtgc agacagaaaa tatcgttacg cattatttta cgatccgttt
       61 ccggcacgac atcacggcag atcacgaagt ggtttattac ggccaagagt accggatccg
      121 gcgaatacgt gacctgaacg gtcagcggcg ttttttatta ctggaatgtg aagaactgcg
      181 tacagcgcga cgacggggtg aatgccatga atcagacagc atttttacac gtcgacttta
      241 aacaaccaga ggagctggag tttaatcgcg cccgtctgcg tcgggcgttt gtgcaaatcg
      301 ggcgcgttta catgcgtgat gccagacgtc tggtgattaa acgcggacgc tccggtccgg
      361 gagagaaccc gggatatcag accgggcgtc tggcccgctc cattggttat tacgttccca
      421 aaaaaacgac gcgtcgcccc ggactgatgg tgaaaatttc ccctgaccag aaaaacgggc
      481 aggggaat
//
LOCUS       DAJTUN010000366          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTUN010000366 DAJTUN010000000
VERSION     DAJTUN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.372"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>480)
                     /gene="aqpZ"
                     /locus_tag="OO470_004146"
     CDS             complement(<1..>480)
                     /gene="aqpZ"
                     /locus_tag="OO470_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6253189.1"
                     /translation="HFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIAS
                     GKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIG"
BASE COUNT          140 a          141 c          115 g           84 t
ORIGIN      
        1 caccgataat gccgccgaca attggcacca cccagaagaa ccacagttgt tctaatgccc
       61 agccgccctg gaagatagca accgcggtgc tgcgcgccgg gttaacagaa gtgttagtca
      121 ccggaatact gattaagtga atcagggtta aggccagacc aatagcgatc ggcgcaaaac
      181 ctgccggagc gaatttgtcg gttgcaccgt ggatcactaa caggaagccc gcactcaata
      241 ccagttcaac taccagcgcg gaaagcatgg aataaccgcc tggtgaatgc tcgccataac
      301 cgttagaagc aaaaccgctg gctgccgcgt caaaacccgt tttaccactg gcaattaaat
      361 acagcagcgc cgctgcaaca ataccgccga caacctgggc aattacgtag ccaacgactt
      421 cttttgccgg aaaacgtccg ccagcccata aaccaatagt gaccgccggg ttaaaatgac
//
LOCUS       DAJTUN010000367          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTUN010000367 DAJTUN010000000
VERSION     DAJTUN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.373"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..90
                     /locus_tag="OO470_004147"
     CDS             <1..90
                     /locus_tag="OO470_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HCR6253190.1"
                     /translation="DIVRGSDLVVTAIAEGRKAADGIMNWLEV"
     gene            274..>480
                     /locus_tag="OO470_004148"
     CDS             274..>480
                     /locus_tag="OO470_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253191.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDR"
BASE COUNT          126 a          119 c          126 g          109 t
ORIGIN      
        1 gatatcgtcc gtggttccga tctggtcgtt accgccatcg ccgaaggtcg caaagcggca
       61 gacggcatta tgaactggct ggaagtttaa gctctgtccc agcgccgcct cctcagaaaa
      121 gagtcggcgc tgaaattctc ctttctttct ggaaatcgcc tcgcataatc tgaagaaaac
      181 caatgtaata cgctcatctg gattgttacc ggtagatgcc cggcgtatag ctgattccat
      241 tattcaacgc agcgccatat aaagggacag ataatggaat cgctctcaga gagaacctca
      301 acaggctacc agcaaatcca caacggcatt attcatctgg tcgatagcgc ccgaacggaa
      361 acggtacgta gcgttaacgc gctgatgacc gcgacgtact gggaaattgg ccgacggatt
      421 gtcgaatttg aacaaggtgg tgaggccaga gcagcttatg gtgcacagtt gattgatcgg
//
LOCUS       DAJTUN010000368          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTUN010000368 DAJTUN010000000
VERSION     DAJTUN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.374"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..280
                     /locus_tag="OO470_004149"
     CDS             <1..280
                     /locus_tag="OO470_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6253192.1"
                     /translation="FKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRN
                     KHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            277..>472
                     /gene="tnpB"
                     /locus_tag="OO470_004150"
     CDS             277..>472
                     /gene="tnpB"
                     /locus_tag="OO470_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6253193.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLC"
BASE COUNT          116 a          120 c          136 g          100 t
ORIGIN      
        1 gtttaaatgg aaaagccagt acgaggacgg cttactgagc gatgatgaca tacaggaatg
       61 catgcctgtc ccggtggcac tgactgatac gccggagccg accagaccag ttacaaatcc
      121 cttctggcgt aacaagcatg atgagcgccc tgagggggct cccggaaacg tcccacggtg
      181 cgagctgcat cttaaatcag gtgtggtaaa actgtttgac cctctcactc cggaactgtt
      241 acgggcgcta atccgcgaaa tgaaaggggg tatccgatga taacgctgcc gaccggtacc
      301 aaaatctgga tcatcgctgg catcacagat atgcgttgtg gcttcaatgg cctggcttcg
      361 aaggtgcaga acacgctgaa agatgacccg ttctccgggc atatcttcgt cttccggggc
      421 cgcagtggca aaatggtgaa aatactgtgg gccgatcgtg acgggttatg cc
//
LOCUS       DAJTUN010000369          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTUN010000369 DAJTUN010000000
VERSION     DAJTUN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.375"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            17..322
                     /locus_tag="OO470_004151"
     CDS             17..322
                     /locus_tag="OO470_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253194.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            322..>468
                     /locus_tag="OO470_004152"
     CDS             322..>468
                     /locus_tag="OO470_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6253195.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTR"
BASE COUNT          149 a          107 c          108 g          104 t
ORIGIN      
        1 cggaggctgg agcataatga ccacttttac cgacaaagaa ctgattaaag aaattaaaga
       61 gcgtatcagc agccttgacg tgcgagacga tattgagcgc cgtgcttatg aaatcgcact
      121 cctatctctg gaagtagaac cagatgaacg cgaagcttat gaattattca tggaaaagcg
      181 ttttggtgac ttagtagatc gtcggagagc aaaaaacggc gataacgaat acatggcatg
      241 ggatatgact ctcggttgga tcgtctggca gcaacgagct ggtatccatt tctcaacaat
      301 gtcacagcaa gaggtgaaat aatggagcca tacagcctca cactcgatga ggcctgtcat
      361 tttctcaaga tatccagacc gactgccatt aactggatac gcacagggcg tcttcaggca
      421 acacgcaaag atcccactaa gaataaatct ccttacctca caacacga
//
LOCUS       DAJTUN010000370          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTUN010000370 DAJTUN010000000
VERSION     DAJTUN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.376"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>463
                     /locus_tag="OO470_004153"
     CDS             <1..>463
                     /locus_tag="OO470_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6253196.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTUN010000371          460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTUN010000371 DAJTUN010000000
VERSION     DAJTUN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.377"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..155
                     /locus_tag="OO470_004154"
     CDS             <1..155
                     /locus_tag="OO470_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6253197.1"
                     /translation="FWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIHAF
                     WVQTRD"
     gene            239..>460
                     /gene="kbp"
                     /locus_tag="OO470_004155"
     CDS             239..>460
                     /gene="kbp"
                     /locus_tag="OO470_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6253198.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAV"
BASE COUNT          108 a          107 c          130 g          115 t
ORIGIN      
        1 gtttctggag aatcgtcatc accatcattc tgccgccgat cggcgtgctg ctcggtaaag
       61 ggttcggttg ggcgttcatt attaatattc tgctgacact gcttgggtat atccccggtt
      121 tgattcacgc gttctgggtg caaacccgcg attaaacctg tgctcacccc cgcggcgtta
      181 acgcgccgcg ttcctctgct acactttctg agtgtttccg ttaacgaatg aggtagctat
      241 gggtctgttc aattttgtta aagatgccgg agaaaaactc tgggatgcgg ttacaggtca
      301 gcacgataaa gacgatcagg cgaagaaggt gcaggagcat ctgagcaaaa ccggtatacc
      361 tgatgccgat aaagtgaaca ttcaaattgc cgacggcaaa gcgacggtca ctggtgacgg
      421 cctgagtcag gaggcgaagg agaaaatcct tgttgcggtg
//
LOCUS       DAJTUN010000372          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTUN010000372 DAJTUN010000000
VERSION     DAJTUN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.378"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            82..351
                     /locus_tag="OO470_004156"
     CDS             82..351
                     /locus_tag="OO470_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6253199.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..>457
                     /locus_tag="OO470_004157"
     CDS             345..>457
                     /locus_tag="OO470_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6253200.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDK"
BASE COUNT          135 a           99 c          123 g          100 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaa
//
LOCUS       DAJTUN010000373          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTUN010000373 DAJTUN010000000
VERSION     DAJTUN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.379"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..223)
                     /locus_tag="OO470_004158"
     CDS             complement(<1..223)
                     /locus_tag="OO470_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6253201.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMC"
     gene            complement(223..>457)
                     /locus_tag="OO470_004159"
     CDS             complement(223..>457)
                     /locus_tag="OO470_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6253202.1"
                     /translation="PFDPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGG
                     TFWFVTELFILFALTVVIFMAKKIWLERQKRND"
BASE COUNT          139 a          125 c          112 g           81 t
ORIGIN      
        1 ggcacatttg cgcaatgcgc gcgctgacaa gcgatcgcac taccggatcg agcggtgagc
       61 gtttgcgctc cagccaccca acaaacattg acaccaaata aaagataaac gggatccgcc
      121 cccaccagcg aatcgggctt agcacctcgc cgtaatggcg acgatgcagc cacgcctgcg
      181 gacgtaaaat gaaaggaatt ttgctaagcg gcggtaaacc catcagtcat tcctcttctg
      241 gcgctcaagc catattttct tcgccataaa aatcaccacg gtcagggcaa acagaataaa
      301 cagttcggtg acaaaccaga aagtaccgcc tgttaacatg ctgcctgccc aggaataaac
      361 aatagtcgcc ggtaattgac caagcccggt ggcgataaaa aacgagcgaa aacgtattga
      421 agtcagaccg gcagcatagc tgattggatc gaaaggg
//
LOCUS       DAJTUN010000374          456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTUN010000374 DAJTUN010000000
VERSION     DAJTUN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.380"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(110..>456)
                     /locus_tag="OO470_004160"
     CDS             complement(110..>456)
                     /locus_tag="OO470_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6253203.1"
                     /translation="RYLLVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGG
                     GDVKLLTVLSLAIDEHELANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLA
                     FLLTYPVFPLFC"
BASE COUNT          175 a           96 c           91 g           94 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgat
//
LOCUS       DAJTUN010000375          454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTUN010000375 DAJTUN010000000
VERSION     DAJTUN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.381"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..178
                     /locus_tag="OO470_004161"
     CDS             <1..178
                     /locus_tag="OO470_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253204.1"
                     /translation="EQAKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADN
                     AADAPTETCFMHYE"
     gene            191..>454
                     /locus_tag="OO470_004162"
     CDS             191..>454
                     /locus_tag="OO470_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6253205.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVA"
BASE COUNT          118 a           86 c          126 g          124 t
ORIGIN      
        1 tgaacaggcg aaagccttaa tggcgcaata tcgggtaccg gtagtcgtgg aagttattct
       61 cgagcgtgtg accaatattt cgatgggcag cgaactggat aacgtcatgg aatttgaaga
      121 tatcgccgat aacgcagcgg atgcaccgac tgaaacctgc ttcatgcact atgaataagg
      181 gagataaatg atgttacgtt tctctgctaa tttatcgatg ttatttggtg aatatgattt
      241 tctcgcccgt tttgagaaag ctgcgcagtg tggttttcgc ggcgttgaat ttatgtttcc
      301 ttatgactac gacattgaag aattaaaaca ggtactggcg agtaataaac tcgaacatac
      361 gctgcacaat ttaccggcag gtgactgggc ggcgggcgag cgcggtattg cctgtattcc
      421 tggccgtgaa gaagagtttc gggatggcgt agca
//
LOCUS       DAJTUN010000376          451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTUN010000376 DAJTUN010000000
VERSION     DAJTUN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.382"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..90
                     /locus_tag="OO470_004163"
     CDS             <1..90
                     /locus_tag="OO470_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6253206.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          141 a          114 c           90 g          106 t
ORIGIN      
        1 cgcaccgccg ataaagattc agctaaaact tttgttcagg aaactctgaa agatacctgg
       61 gagtctgctg acgagtggtt tgttcactaa ttagcacgta aaatcagtaa cggctggaaa
      121 tcattcaata ctcgcactat cggaagttca ccagccagcc gcagcacgtt cttgcatacg
      181 acgtgtctgc ggtctcaact ccgcccgccc ccgccaatca tgattgggca gtaccagtac
      241 agcactagca aagacaggaa attagcagtc tcagcaaaac accgaccaga cggtgagaag
      301 acaaaaagat acgcaaagat gccgcggttc cagaagaata aaaaaccgct aatggacagc
      361 ctccttaaca cagtttttta tatacatgat tcactgatac cagtggcatt aatagctatt
      421 atatagcttc agctcctttt ttcatacctt a
//
LOCUS       DAJTUN010000377          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTUN010000377 DAJTUN010000000
VERSION     DAJTUN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.383"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            324..>448
                     /locus_tag="OO470_004164"
     CDS             324..>448
                     /locus_tag="OO470_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6253207.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEI"
BASE COUNT          150 a          100 c           68 g          130 t
ORIGIN      
        1 gttgcagata atgttatttt tgatgcgtac gcgttataaa tatcaaaaac acaaaccaga
       61 taaatatctt atcaatatgc aacaaagttt gaattattca atttatagca gcacaaccaa
      121 acgccaaaaa ataataacca agagctataa ataaagatta aaaatatttt cagtgaatat
      181 gaaattattg ctatttctat tacaaagatg tcctggtctc tctttcattc tcttcttgtc
      241 aacatcgcga caactttcgt aaaacactcc tgccccgctt caggtatact gtgcacctta
      301 attccacaaa catcaggcag accatgacag acttaatcca acgccctcgt cgcctgcgca
      361 aatctcctgc gctgcgcgct atgtttgaag agacaacact tagccttaac gacctggtgt
      421 tgccgatctt tgttgaagaa gaaattga
//
LOCUS       DAJTUN010000378          445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTUN010000378 DAJTUN010000000
VERSION     DAJTUN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.384"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>445)
                     /gene="treF"
                     /locus_tag="OO470_004165"
     CDS             complement(<1..>445)
                     /gene="treF"
                     /locus_tag="OO470_004165"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="cytoplasmic; catalyzes the hydrolysis of trehalose
                     to glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR6253208.1"
                     /translation="YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLL
                     TPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYL
                     EQHKMIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGE"
BASE COUNT           98 a          137 c          105 g          105 t
ORIGIN      
        1 gttcgccgta caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct
       61 gcaacggata ctcgccaccg ccgccttcgc ggggaacacc atcggcaata tggtatttct
      121 cgatcatttt gtgctgttcc agatagaact gattcaccgt tttcagccag ctgcgcgcga
      181 tttcatcacc cagaaggtca tcgccataca ttttaaatcc ctgaattgcc atccattgta
      241 acggtgccca gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccaaaa
      301 tcccgccagg tgtcagtaac cggctgcgta cggcgtttgc cagacgatcg gcctgttcat
      361 ggttcgccat gccgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgtt
      421 cgcgtcgcca gtcgtaatcg cggta
//
LOCUS       DAJTUN010000379          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTUN010000379 DAJTUN010000000
VERSION     DAJTUN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.385"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT          146 a           70 c           88 g          140 t
ORIGIN      
        1 tgaatatttc agagttggag ttcgaatgtt gacacctgag tatttcctgc taaaaatagc
       61 cctgctttac ggatggttgt attgcaataa cacgaaagta gtaagagatt aaaggtatct
      121 gtcagcgata actctgcaag tgatgacatt ttgtaaacca aataatgtaa atcagcaatg
      181 tatcttttca cttaattata acaaatgtgg tattcactaa gaatctgata cgagcttaca
      241 tcacgactat tttagtctca tcgcgggata cagggatgca ccgatggatc tcagcacacc
      301 gtaatgcaat acctgaacaa atagaacagt aaagtggttc agtattaatg aaagctctta
      361 ttttccgttg ggaaaactta aaatagatga gaaggctttt tgatttctta tgtgatctca
      421 tttcgagtat tgagaaattt gtga
//
LOCUS       DAJTUN010000380          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTUN010000380 DAJTUN010000000
VERSION     DAJTUN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.386"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>442
                     /locus_tag="OO470_004166"
     CDS             <1..>442
                     /locus_tag="OO470_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HCR6253209.1"
                     /translation="MPDDVVDKLKQLKDAETSSMGKIIYHTMFDNMQKAIDLNRPACQ
                     DTGEIMFFVKVGSRFPLLGELQGILKQAVEEATVKAPLRHNAVEIFDEVNTGKNTGSG
                     VPWVTWDIIPDNDDAEIEVYMAGGGCTLPGRSKVLMPSEGYEGVV"
BASE COUNT          125 a          108 c          121 g           88 t
ORIGIN      
        1 gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa acgtcgtcga
       61 tggggaaaat tatctaccat acgatgttcg acaacatgca aaaagcgatt gacctgaatc
      121 gtcctgcctg tcaggacacc ggggagatta tgttcttcgt taaagtcggt tcccgcttcc
      181 cactgcttgg cgagctgcaa ggcatactca aacaagccgt ggaagaggca accgtcaaag
      241 cgccactacg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc aaaaataccg
      301 gtagcggcgt accgtgggtc acctgggaca tcatccccga caatgacgat gcggaaatcg
      361 aagtttacat ggcaggcggc ggctgcacgc tacctggccg ctcgaaagtg ttaatgccgt
      421 cagaaggcta cgaaggcgtg gt
//
LOCUS       DAJTUN010000381          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTUN010000381 DAJTUN010000000
VERSION     DAJTUN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.387"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..101)
                     /locus_tag="OO470_004167"
     CDS             complement(<1..101)
                     /locus_tag="OO470_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6253210.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAEL"
     gene            complement(228..>431)
                     /locus_tag="OO470_004168"
     CDS             complement(228..>431)
                     /locus_tag="OO470_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="With FlhD is involved in the activation of class 2
                     flagellar genes and as well as a number of other genetic
                     systems; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FlhC"
                     /protein_id="HCR6253211.1"
                     /translation="FVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPSRAVKR
                     RKLSQNPADIIPQLLDEQRVQAV"
BASE COUNT          120 a          102 c          111 g           98 t
ORIGIN      
        1 agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata accgccgaaa
       61 actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt
      121 tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggaa aaaaaaagca
      181 gcggtacgtc gttaccgctg ctggaatgtt gcgccacacc gtatcagtta aacagcctgt
      241 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
      301 ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
      361 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
      421 ctttcaacaa a
//
LOCUS       DAJTUN010000382          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTUN010000382 DAJTUN010000000
VERSION     DAJTUN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.388"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            127..>431
                     /locus_tag="OO470_004169"
     CDS             127..>431
                     /locus_tag="OO470_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253212.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     "
BASE COUNT          138 a           77 c           88 g          128 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tggcgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca c
//
LOCUS       DAJTUN010000383          427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTUN010000383 DAJTUN010000000
VERSION     DAJTUN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.389"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            209..>427
                     /locus_tag="OO470_004170"
     CDS             209..>427
                     /locus_tag="OO470_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6253213.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGAL"
BASE COUNT          102 a          107 c          116 g          102 t
ORIGIN      
        1 ttataatggg gtgttagcca gccagacccg gcatgattac tgcccccagt cgtccatgat
       61 ccgggggggg atgtcaccgg gtctggtggg gcgctggtaa ccgctaatag gggtcaggtc
      121 aggcactttt gccgggaccg tctgtaacgt ggatgccggt acctgctccc cgtggttatc
      181 tggttaaccc atatacaagg gagacagaat gaccgaatcc agcgattacg aatccgtcca
      241 ggtctttatc ggcgttgatg tcggtaaaga tacgcatcac gctgttgcca ttaatcgttc
      301 aggtaaacgc ctgttcgata aagcattacc caacgacgaa aacaaactca ggtcgctaat
      361 atctgacctg aaacaacatg gtcagatact gctggttgtt gatcagccag ctaccatcgg
      421 tgcgtta
//
LOCUS       DAJTUN010000384          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTUN010000384 DAJTUN010000000
VERSION     DAJTUN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.390"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..289)
                     /locus_tag="OO470_004171"
     CDS             complement(<1..289)
                     /locus_tag="OO470_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6253214.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPD"
     gene            complement(301..>423)
                     /locus_tag="OO470_004172"
     CDS             complement(301..>423)
                     /locus_tag="OO470_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6253215.1"
                     /translation="TDALHATRDEPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          122 a          122 c           90 g           89 t
ORIGIN      
        1 catcaggggt gatacgatcc acacagaaag actcacgacc aatcatcagc gaatcattgc
       61 gacacagccg taaaatatct gctgttttca caaacaggga tggtaaagag tcttcaatac
      121 gcacaccgcc ggccaccaga gaaatatttt caggatcatc aaaaatcccg gaaatcatga
      181 tcccttttag ccggcctgac gtaatgaccg ctctaatccc catattgcga ataatggcct
      241 tgtctgcaag atcaagtgca acatcaaaca gattatcgaa atcagccacg ctgcctcccg
      301 ttattgcatt ctggccaggc cacgttctgt cattccggct gccacaccgg caaagacacg
      361 gaacgccatt cccggcggca caaatgccag aggttcatcc cgcgtggcat gaagtgcatc
      421 ggt
//
LOCUS       DAJTUN010000385          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTUN010000385 DAJTUN010000000
VERSION     DAJTUN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.391"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(1..246)
                     /locus_tag="OO470_004173"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="OO470_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT          130 a          107 c           73 g          113 t
ORIGIN      
        1 gccgccaagg aaataaattc ccaacgggat tgccagcaac acagtaaaga ccaggctata
       61 aacaaaaatc atcgatgact aaatcacata cattgatgtc gtccactcag acagcggaat
      121 attgtactgt tctataacgc cgccgatggt cgcgcgagtc acaaccgacg ccgcaataat
      181 gaaaaccgcc aacaaaccaa agagaaattt cttccctttc gctgacttca gtttcgccat
      241 aatcatgatg taatccttgt gcagacaaat ttcttgttac ctaaacgtaa caataccatg
      301 acgtttcacc gttgtctgct ctgaaaacct cacctcttag ctggatttac aatcaacgca
      361 ttgattagtt agctaatgtt gctacttcct tccctgacta ttgatgttct gaatgaattg
      421 tcg
//
LOCUS       DAJTUN010000386          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTUN010000386 DAJTUN010000000
VERSION     DAJTUN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.392"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>422)
                     /locus_tag="OO470_004174"
     CDS             complement(<1..>422)
                     /locus_tag="OO470_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6253216.1"
                     /translation="TYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPS
                     GNTKYNCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYS
                     QPMADVYIRGDITAPQSCEINNLKPVCFDFKEIPAADF"
BASE COUNT          136 a           86 c           69 g          131 t
ORIGIN      
        1 aaaatctgca gctggtattt ctttaaaatc aaaacaaact ggctttaaat tatttatttc
       61 gcaactttgc ggagctgtaa tatcgccccg aatataaaca tcagccatcg gctgtgagta
      121 atccaccggg gtgtcacggc taattgttcc atacaatgtc actatctttg tttcaggaat
      181 aatcaatttt cctgcaaatg tcttcttgat ataaaaatat actatcgctt tagccccact
      241 ggatatactt aaaggttcaa tgcggttgca attgtacttt gtattaccag acgggttgtt
      301 agcatgatat tcaaaaggtg cattcacata cccaacccca agaatatcta tagcaatccc
      361 aacatcaagg ttcggattaa gatcataata attaatatta gagtgaaccc cagcataagt
      421 ta
//
LOCUS       DAJTUN010000387          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTUN010000387 DAJTUN010000000
VERSION     DAJTUN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.393"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..106
                     /locus_tag="OO470_004175"
     CDS             <1..106
                     /locus_tag="OO470_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6253217.1"
                     /translation="ASSVIHATGGDRVATSQTDRTAWHPDITRDKTRE"
     gene            103..>421
                     /locus_tag="OO470_004176"
     CDS             103..>421
                     /locus_tag="OO470_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6253218.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          129 a          105 c          109 g           78 t
ORIGIN      
        1 cgccagctca gttatccatg ctactggagg ggatcgcgtg gcaacatccc aaacggacag
       61 aacggcctgg catccggata taacccgtga taaaacaagg gaatgaacaa tgaactcccc
      121 gatgatattg agctgcttaa agccatgttg cgtaagcaac agagtcggct tcgacagtat
      181 gcctgtcagg tcgcgggcta tgagcaggaa attgaacggc tgaaagcgca actcgacagg
      241 ttgcgtcgta tgttgttcgg ccagagttca gagaaaaagc gtcataagct tgaaaatcag
      301 atccgacagg cagaaaaacg actgtcggaa ctggaaaacc ggctgaacac agccagaaat
      361 cttctggaag atgcatcgtc agtcacagat tcacctgaca ccagtccccc gtcagaaaac
      421 c
//
LOCUS       DAJTUN010000388          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTUN010000388 DAJTUN010000000
VERSION     DAJTUN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.394"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>420)
                     /locus_tag="OO470_004177"
     CDS             complement(<1..>420)
                     /locus_tag="OO470_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6253219.1"
                     /translation="LTDRGEGFVHDLTSLARDVATGVLARSMDVDIYNLHPAHAKRVE
                     EIIAENKPPFSVFRDKFITMPGGMDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLT
                     ETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEK"
BASE COUNT           94 a          104 c          114 g          108 t
ORIGIN      
        1 tttttcttca tcatcctgtt ttccttcttc tgttacacgt tgcggcatcg gggcagagga
       61 gcgaccgcag gcaatatcca cgatttccgg atcagggttg gcatgatcgg tttcagtcag
      121 taccttgttc agatattcag tgacgtgagc ggggatgacc tcgataccaa tcggtgcttc
      181 tttcactgac gcaaccacga tggcgcggga ataatccatc ccgccaggca tggtgatgaa
      241 tttgtcgcgg aaaacagaaa agggcggttt attttcagcg ataatttcct cgacacgttt
      301 agcgtgtgcc ggatgaaggt tataaatgtc cacgtccatt gaacgagcca gtacgccagt
      361 ggctacgtcg cgcgccagtg acgtcagatc gtgaacgaaa ccttcgccgc gatcggtgag
//
LOCUS       DAJTUN010000389          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTUN010000389 DAJTUN010000000
VERSION     DAJTUN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.395"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            105..>416
                     /locus_tag="OO470_004178"
     CDS             105..>416
                     /locus_tag="OO470_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6253220.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IK"
BASE COUNT          102 a          104 c           86 g          124 t
ORIGIN      
        1 gttttttcgc tatacccctt tatagattct gaattctcac ctgtcggctg ttttctcctc
       61 atctcatgtg acatattccc atactttact caaggaagat tgtcatgaaa gcacctgttt
      121 cattaacctc atttcgccca caaaaatctc ttgctatcgc cattggcgtg cttgccgtgg
      181 ttgtgctgcc atttctctcg tattacaccg ttaatgaagg tgaacgcggc attttgctgc
      241 gctacggaaa aatcgtaaaa gttgccgaac cggggctggg ttttaagatc ccttttatgg
      301 aaagcgtgga gaaaatttcc acgcgtaatc aggctgtggt ctatcaggga ttacaggcct
      361 acagccgcga ccagcaaccg gcacaaatga cagtatcagt cagcttccac attaaa
//
LOCUS       DAJTUN010000390          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTUN010000390 DAJTUN010000000
VERSION     DAJTUN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.396"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            57..>416
                     /locus_tag="OO470_004179"
     CDS             57..>416
                     /locus_tag="OO470_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6253221.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILGSRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTAD"
BASE COUNT          148 a           96 c           94 g           78 t
ORIGIN      
        1 tcactgaagc tatacaggcc aacattgagt tattttcagg acacaaacac aagcctctga
       61 cagcgagaat caacagtgat aattctgtta cgttacattc atggcttgat cgctacgaaa
      121 aaatcctcgg cagcagagga atcaagcaga agacactcat aaattacatg agcaaaatta
      181 aagcaataag gagggggctg cctgatgctc cacttgaaga catcaccaca aaagaaattg
      241 cggcaatgct caatggatac atagacgagg gcaaggcggc atcagccaag ttaatcagat
      301 caacactgag cgatgcattc cgagaggcaa tagctgaagg ccatataaca acaaacccgg
      361 tcgctgccac tcgcgcagca aaatcagagg taaggagatc aagacttacg gctgac
//
LOCUS       DAJTUN010000391          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTUN010000391 DAJTUN010000000
VERSION     DAJTUN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.397"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            192..>415
                     /gene="ydeI"
                     /locus_tag="OO470_004180"
     CDS             192..>415
                     /gene="ydeI"
                     /locus_tag="OO470_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6253222.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT          130 a           80 c           82 g          123 t
ORIGIN      
        1 tccgattatt ctgaaaacat gtttaagact cttcaatctt cttaacttta gtgagtcaaa
       61 ttctctgaat atttgacagc tagtgggctg attttaaatg ccttataaca attggcagta
      121 ttcttatttg gcaattataa aaatagcaac cagacttaat aagtcaaacc aaacgcaatt
      181 aaggagttag tatgaaattt cagtccattg tattagcaag ttttcttgtc atgccttatg
      241 cgttggcaga tgaccagggc ggtttaaaac aggatgcagc gccaccgccg cctcatgcaa
      301 tagaagatgg ctatcgcgga accgatgatg caaaaaaaat gaccgttgat ttcgcaaaaa
      361 acatgcacga tggcgcttcg gtttcactac gcggtaattt gatttctcac aaagg
//
LOCUS       DAJTUN010000392          411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTUN010000392 DAJTUN010000000
VERSION     DAJTUN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.398"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..>411
                     /locus_tag="OO470_004181"
     CDS             1..>411
                     /locus_tag="OO470_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6253223.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVS"
BASE COUNT          102 a          107 c          123 g           79 t
ORIGIN      
        1 atgaaaaata ttgcgacagg aggcgttctg gagcgtatcc gcagactgac cccaccacat
       61 gtaaccgccc cattcagaac ggttgcggag tggcgcgagt ggcaacttgc tgaaggccag
      121 aaacgttgcg aggagatcaa ccgcctgaat cgtcagttgc gggtggaaaa aatcctccgc
      181 tccggcatcc agccgttgca ccggaagtgt tcgtttgcga attaccgggc gcagaacgac
      241 ggtcagcgac atgcactgag tcaggcgaaa tccatagctg acgaactgat gacaggctgt
      301 acgaattttg tgttcagcgg taagcccggc accggaaaaa atcatcttgc agccgcgatt
      361 ggcaaccggc tgatggcgaa ggggcgtagc gtgattatcg tcaccgtgtc c
//
LOCUS       DAJTUN010000393          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTUN010000393 DAJTUN010000000
VERSION     DAJTUN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.399"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>408
                     /locus_tag="OO470_004182"
     CDS             <1..>408
                     /locus_tag="OO470_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6253224.1"
                     /translation="QQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRND
                     SVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRA
                     LEFAKKEGNTLVIVTADHAHASQVVAPDTKAPGL"
BASE COUNT          113 a          109 c          108 g           78 t
ORIGIN      
        1 tcagcaaaaa cccctgcttg gcctgtttgc tgacggcaat atgccagtgc gctggctagg
       61 gccgaaagca acgtaccacg gcaatatcga taagcccgca gtcacctata cgcctaatcc
      121 gcaacgtaat gacagtgtac caaccctggc acagatgacc gacaaagcca ttgaattgtt
      181 gagtaaaaat gagaaaggat ttttcctgca agttgaaggt gcgtcaatcg ataaacagga
      241 tcacgctgcg aacccttgtg ggcaaattgg cgagacggtc gatctcgatg aagccgtaca
      301 acgggcgctg gaattcgcta aaaaggaggg taacacgctg gtcatagtca ccgctgatca
      361 cgcccacgcc agccaggttg ttgcgccgga taccaaagcg ccgggcct
//
LOCUS       DAJTUN010000394          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTUN010000394 DAJTUN010000000
VERSION     DAJTUN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.400"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>405
                     /locus_tag="OO470_004183"
     CDS             <1..>405
                     /locus_tag="OO470_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6253225.1"
                     /translation="DTRMVPQASAWAINRVAQKAVSVATRQVAGNTVAGDNQVKGIPL
                     KLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRGAFVQ
                     QLANGRWHVLRCLPEARFATGHDHQGRLRKNR"
BASE COUNT           97 a          101 c          128 g           79 t
ORIGIN      
        1 ttgatacccg tatggtgcca caggccagcg catgggcgat aaaccgtgtg gcacagaaag
       61 cggtctcggt tgccacccgg caggttgccg ggaataccgt tgcgggcgat aaccaggtga
      121 aagggatccc cctgaaactg gtacgtcagc gtgtccgggt gtttaaagcc agtccgtcag
      181 gaaaaatgac ggccaggatc cgcgttaacc ggggcaatct gcccgccatc aaactgaaca
      241 caacacggcg gcgtgctggt gaaggactga gagtgggaaa atactttttc cggggggcat
      301 ttgttcagca actggcgaat ggccgctggc atgttctgag gtgtcttcct gaagcgcgtt
      361 ttgcaacagg gcatgaccat cagggcaggc taagaaaaaa tcgtc
//
LOCUS       DAJTUN010000395          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTUN010000395 DAJTUN010000000
VERSION     DAJTUN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.401"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            150..>402
                     /locus_tag="OO470_004184"
     CDS             150..>402
                     /locus_tag="OO470_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253226.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTEST"
BASE COUNT          133 a           87 c          110 g           72 t
ORIGIN      
        1 gacttaattt aggcttaaac accttacatt tacaaggtac tcctgtgggg ggccctgcca
       61 cggagcgtcg caggagtgag tgatgaggaa gaattaaagc aacctattgt gacaggcagc
      121 gataacagta aatcacagga agcataatca tggcaaaacc ggactgggag gccatcgaat
      181 cggcataccg ggccggagtc cttagcctcc gtgatatagg cgagaaatac ggcgttacag
      241 aaggggctat caggaagagg gccaaaaagc ttgattgggc acgcagtggc ggtacgcagg
      301 tttgcaaaaa tggtacgcaa aaaaggaaag tgcgtaccag cagaaagcct gccattactg
      361 gccttacaca aaaaagtacg caactaaaaa cagaatctac ac
//
LOCUS       DAJTUN010000396          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTUN010000396 DAJTUN010000000
VERSION     DAJTUN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.402"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..288
                     /locus_tag="OO470_004185"
     CDS             <1..288
                     /locus_tag="OO470_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6253227.1"
                     /translation="FFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPP
                     MDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          133 a           83 c           83 g          103 t
ORIGIN      
        1 ttttttgcga cctataaagc gacattacca atcattggac gtctgatgaa catcagtcag
       61 gctatgatta taccactttt cccggtttat gatgaaaaaa aacatctact gactattgaa
      121 attcggccac caatggatgc ttgcattgcc agcgcggaca ataaaacgat tgcccgacaa
      181 atgaacaaaa cagtggaaat tttggtgggg ccacatccgg aacagtatgt ctgggtttta
      241 aaattgttaa aaacacgcaa atcaaacgaa gcggacccgt acccttgaaa ttataaagta
      301 caagtatttc cactagttgt ttgtaagcgt acagcctgaa ccgtctggtc agaatctgac
      361 gaattagaca aagtggtgtc caccaaataa gtagtgggaa cc
//
LOCUS       DAJTUN010000397          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTUN010000397 DAJTUN010000000
VERSION     DAJTUN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.403"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            195..>400
                     /locus_tag="OO470_004186"
     CDS             195..>400
                     /locus_tag="OO470_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase"
                     /protein_id="HCR6253228.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVR"
BASE COUNT          113 a           81 c          105 g          101 t
ORIGIN      
        1 attcacattt tatctgctgg gcgcactatt agtggtagtg gctggtggaa tctatgcgct
       61 gcaaatgaag ggcaaaagcg gtgtcacacc tgtagcggca aaagaaattc attaatacga
      121 cacgtttcat taagattttc ctcaggtaac cacctatagt cattgtcgtt aataaaaatg
      181 acaggcgata gggtatggca gtttcagcaa agtacgacga atttaatcac tggtgggcaa
      241 cagagggcga ctgggttgaa gagcctaact atcgccgtaa tggtatgagc ggggtgcaat
      301 gcgtcgagcg caacggcaaa aagctgtatg taaagcgcat gacgcatcat ctgtttcatt
      361 cagtacgtta tccgttcggc cgaccaacga ttgtccgtga
//
LOCUS       DAJTUN010000398          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTUN010000398 DAJTUN010000000
VERSION     DAJTUN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.404"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            29..>397
                     /locus_tag="OO470_004187"
     CDS             29..>397
                     /locus_tag="OO470_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCR6253229.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQ"
BASE COUNT          110 a           91 c           90 g          106 t
ORIGIN      
        1 cttgcctggc agaaataagg agtcataaat gggatattta aataacgtca ccggttaccg
       61 cgaagattta ctggctaacc gtgcgattgt taaacacggt aatttcgcac tgttaacccc
      121 ggatggtctg ataaaaaata ttattccgga ctttgaaaat tgtgacgcga caatcctctc
      181 cacgccaaag ctgggtgcct cttttgttga ttatctggtc acactgcatc aaaacggtgg
      241 caaccaacag ggcttcggtg gcgaaggcat tgaaacgttc ctgtatgtga tctctggaaa
      301 tatcactgcc aaagccgaag gcaaaacatt tgccttaagc gaaggtggct atctttattg
      361 cccaccaggc tccttaatga cgtttgttaa cgcccag
//
LOCUS       DAJTUN010000399          393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTUN010000399 DAJTUN010000000
VERSION     DAJTUN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.405"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..368
                     /gene="yhaJ"
                     /locus_tag="OO470_004188"
     CDS             <1..368
                     /gene="yhaJ"
                     /locus_tag="OO470_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6253230.1"
                     /translation="PDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLDKQP
                     RLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWRRDS
                     MGEAKSWCLREIPKLFAGK"
BASE COUNT           95 a           88 c          123 g           87 t
ORIGIN      
        1 cgcctgatca cccgattcat caggagccgg aaccgttatc tgaagtgacg cgtgtgaaat
       61 atcgtggaat tgcggtagcg gataccgctc gtgagcgccc ggtgttgacc gtacagctgc
      121 tggacaaaca gccgcgctta acggtgagca cgattgaaga taaacgtcag gcattactgg
      181 cggggcttgg cgtggcgacg atgccgtatc cgatggtcga aaaagatatt gcggaagggc
      241 ggttgcgtgt cgtcagcccg gaatcgacca gcgagatcga tattattatg gcctggcgtc
      301 gtgacagtat gggggaagcg aaatcctggt gtctgcggga aattccaaag ttgttcgctg
      361 gaaaataatc tattaagcac ctttcacaag gtg
//
LOCUS       DAJTUN010000400          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTUN010000400 DAJTUN010000000
VERSION     DAJTUN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.406"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>391)
                     /locus_tag="OO470_004189"
     CDS             complement(<1..>391)
                     /locus_tag="OO470_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6253231.1"
                     /translation="MFALAREHADKTGREAVMFSSFLRAQVSLAWVIGPPLAYALAMG
                     FSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDTLLLFVICTL
                     MWGSNSLYIINMPLFIINELHLPEKLAG"
BASE COUNT          109 a          108 c           97 g           77 t
ORIGIN      
        1 ccggctagtt tctcgggaag atgcagttcg ttgataataa atagcggcat gttgatgatg
       61 tacaggctgt tcgagcccca catcaatgta caaatgacaa acagcagtag cgtatcgcga
      121 cggttacggc gcggcgcttc aaccgtgccg gtcgccagcg gaagctcttt ttgcatcgac
      181 ggtaaaaaca gccacaccat cacgccgcaa acaataaacg ctaccgctgc gctcagatac
      241 attaccgtaa agctgaaacc catcgctaac gcataagcca gcggtgggcc aatgacccat
      301 gccagtgaaa cctgagcgcg taaaaaagag ctgaacatca ccgcctcacg tccggttttg
      361 tcggcatgtt cacgggcaag ggcaaacatt t
//
LOCUS       DAJTUN010000401          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTUN010000401 DAJTUN010000000
VERSION     DAJTUN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.407"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>388
                     /locus_tag="OO470_004190"
     CDS             <1..>388
                     /locus_tag="OO470_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6253232.1"
                     /translation="VVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPL
                     TDLTTDYLYEGAQAWYPHYSWHSDGRNILYAGEVVQNPPGTPPVDVASFKAWGDFAAD
                     KHSLYFEGKRTDDNGGGNSLDIKTLHQ"
BASE COUNT          105 a           97 c           91 g           95 t
ORIGIN      
        1 gtggtgtacc tgcgccctta taaagaaaca aatctttctc tacctgacgt gaattacaag
       61 agtttgcgtc ggttacccaa tttattaata gacccgacaa cactagacga atgggataaa
      121 gaaccgcctc tgactgattt gacaaccgac tacctctatg agggcgcaca ggcctggtat
      181 cctcattatt catggcatag cgatggtcgc aatatcctct acgcgggtga agttgtgcaa
      241 aaccctcccg gcacacctcc cgtcgatgtc gcctcgttca aggcatgggg tgattttgcc
      301 gcagataaac acagtctcta ttttgaaggc aagcgcaccg atgacaatgg tggtggaaat
      361 agcttggata tcaaaacgct gcatcagg
//
LOCUS       DAJTUN010000402          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTUN010000402 DAJTUN010000000
VERSION     DAJTUN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.408"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            283..>388
                     /locus_tag="OO470_004191"
     CDS             283..>388
                     /locus_tag="OO470_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253233.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEG"
BASE COUNT          119 a           82 c           79 g          108 t
ORIGIN      
        1 tgaatgcaga ccgaaagtaa cgtcggccag gggaagcggc gaggtaaatc aagggttatt
       61 gaagtcataa tcatttcagt agaaaaacta ggcctcgatt ttaaatgcaa atagcaatca
      121 tattcaataa ggcaagacga tcaggatcag tgaaaatgtc atttttggcg taattaccct
      181 tactttatgc atgaccttaa caaagacatc atctttccgt tgcagatctt cgccctttca
      241 aataacgtac tttacaactt tcccgaacaa ggagttgtgc ccgtgttgta tgttatttac
      301 gctcaagaca aagctgatag cctcgaaaaa cgcctttccg ttcgtccggc acatttagca
      361 cgtttacagt tactgcatga tgaaggtc
//
LOCUS       DAJTUN010000403          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTUN010000403 DAJTUN010000000
VERSION     DAJTUN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.409"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(88..>387)
                     /locus_tag="OO470_004192"
     CDS             complement(88..>387)
                     /locus_tag="OO470_004192"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6253234.1"
                     /translation="GGIGIAPGANIGDECALFEATHGTAPKYAGQDKVNPGSIILSAE
                     MMLRHMGWTEAADLIVKGMEGAINAKTVTYDFERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT           99 a           97 c           85 g          106 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgcc
//
LOCUS       DAJTUN010000404          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTUN010000404 DAJTUN010000000
VERSION     DAJTUN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.410"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..274
                     /locus_tag="OO470_004193"
     CDS             <1..274
                     /locus_tag="OO470_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253235.1"
                     /translation="DEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIYVKTEGKAEAG
                     FLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT          107 a           88 c          100 g           92 t
ORIGIN      
        1 tgacgaagta cggcaagcca tgttgaaagc agtggcactg gcgtttaaga aaatgcatag
       61 cgttaatcgt cagcatggct gttgctatgt tcgccatatc tatgtgaaaa cagaaggtaa
      121 ggcagaagct ggttttcttg atctggagaa aagtcgtcgc cgcttgcgtc gcgataaagc
      181 gattaatcat gacttccgcc agctggaaaa atatctggag ccgatcccta aagcggactg
      241 ggagcaggtg aaagcgtatt actacgccat gtaatcgttg taggccagat aatacgcgta
      301 agcgtcgtat ctggcgctag tacccgatca caactcgatt tcaatatcgc cttttgcccg
      361 gcaacaacag ggcaaaattt cccccgg
//
LOCUS       DAJTUN010000405          386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTUN010000405 DAJTUN010000000
VERSION     DAJTUN010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.411"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT          103 a          102 c           87 g           94 t
ORIGIN      
        1 tgggggttat cttcatagtg ccttgtacat ggcaatccat catctccgaa cctgagcaca
       61 gtaaaaacga tatcgcagac tgccatcacg tcggaagcaa taaccgaata tctggatctg
      121 gattggttca ggcagcagca tctgacatca caaaatccat tcgatatcat tcctgttcat
      181 ggagcctcct ccaccggaga aggtgcggaa aaaggaaatg ccctgacccg actttatcag
      241 gaataccgcg aggcgttgtt atctcccgaa caagatctta atcgccatgc tgatcctttt
      301 ccttttaacg aagagccctc ctcgcaggat ctggattcat tgcgtgacct ggtcaccgaa
      361 gccgagacat tgcaggatat ggtcac
//
LOCUS       DAJTUN010000406          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTUN010000406 DAJTUN010000000
VERSION     DAJTUN010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.412"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>385
                     /locus_tag="OO470_004194"
     CDS             <1..>385
                     /locus_tag="OO470_004194"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6253236.1"
                     /translation="RVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTRDEKLIACG
                     GIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYEALFRQCGF
                     STLTCVPGVMVLMENSATRLKRYAES"
BASE COUNT          109 a           95 c           93 g           88 t
ORIGIN      
        1 cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc ctgcatgaaa
       61 atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc gatgaaaagc
      121 ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc agtaaatccg
      181 ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc gcctatgagc
      241 ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg ttccgccagt
      301 gcggtttttc cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa aacagcgcca
      361 cgcgactgaa acgctatgcc gaatc
//
LOCUS       DAJTUN010000407          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTUN010000407 DAJTUN010000000
VERSION     DAJTUN010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.413"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..330
                     /locus_tag="OO470_004195"
     CDS             <1..330
                     /locus_tag="OO470_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253237.1"
                     /translation="TYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPG
                     KEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTS
                     KTAPQAK"
BASE COUNT          114 a          101 c           90 g           79 t
ORIGIN      
        1 acctattccc aaccggaatg gaaacataac ttgttcgacc attatctggc ggtgctttac
       61 cgctttaaag atgaaagtgg caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa
      121 gcaacgccgg gtaaagaaat agaggcgatc actcgccgaa tgcttgattt cagcccacga
      181 ctgaaaaaac tcgcgggtgt gccatgtcag gtctatgttc gcaccgtagc ggcgaataac
      241 gcccaaccgc taacccagga tcaatgctta cgcgcgctgc atcacttacg tgtacaatcc
      301 accagcaaaa ctgcaccaca ggcgaaataa tctcatcttt gctaatgcga ggctggagcc
      361 atccagcctg aaatgactga taac
//
LOCUS       DAJTUN010000408          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTUN010000408 DAJTUN010000000
VERSION     DAJTUN010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.414"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..354
                     /gene="malI"
                     /locus_tag="OO470_004196"
     CDS             <1..354
                     /gene="malI"
                     /locus_tag="OO470_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6253238.1"
                     /translation="SQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQS
                     GESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEET
                     HSRNLIIPARLIAAK"
BASE COUNT           95 a           98 c           97 g           88 t
ORIGIN      
        1 agccagaagc aagccgcgga agctatcacg gcgcttttac gtcataaccc gaccatcagt
       61 gccgtggttt gctataacga aactattgcg atgggggcat ggtttggttt gctgaaagca
      121 gggcggcaaa gcggggaaag cggagtcgat cgttactttg agcaacaggt ttcgctggcg
      181 gcatttaccg atgcgacacc aaccacactt gatgatatac ctgttacctg ggccagcacg
      241 ccagcgcggg aacttggtat cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa
      301 gagacgcatt cacgcaatct tattattccc gcccggctca ttgcggcgaa ataatcctct
      361 ctttatctgc tatacttg
//
LOCUS       DAJTUN010000409          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTUN010000409 DAJTUN010000000
VERSION     DAJTUN010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.415"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>378)
                     /locus_tag="OO470_004197"
     CDS             complement(<1..>378)
                     /locus_tag="OO470_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253239.1"
                     /translation="DRGVNEMSAALKRRFDFETVFPIMDFAQELELVASASARLLAHS
                     GIPHKVPDAVLELLVRTFRDLRANGEKKTSMDTLTAIMSTAEAVNVAHAVGVRAWFLA
                     NRAGEPADLVDCIAGTIVKDNEE"
BASE COUNT           83 a          109 c          102 g           84 t
ORIGIN      
        1 tcttcttcgt tatctttgac gatggttccg gcaatgcagt ccaccaggtc ggctggctca
       61 cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac gttcacggct
      121 tcagcggtgg acataatcgc tgttagtgta tccattgacg ttttcttttc gccgttggca
      181 cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg cgtcaggcac tttatgcgga
      241 ataccgctat gggccagcaa acgcgccgaa gcactggcga caagctccag ctcctgggcg
      301 aaatccataa tcggaaagac agtttcaaaa tcgaagcgac gctttagcgc agcactcatt
      361 tcattgacgc cgcgatcg
//
LOCUS       DAJTUN010000410          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTUN010000410 DAJTUN010000000
VERSION     DAJTUN010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.416"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..83)
                     /locus_tag="OO470_004198"
     CDS             complement(<1..83)
                     /locus_tag="OO470_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6253240.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEK"
BASE COUNT          130 a           61 c           56 g          130 t
ORIGIN      
        1 tctttttcca taaagctaca ccaatctgta ggttgtagat ctctttctat tattaataga
       61 tataagaact gtactgttgt caatatgaaa ttccgcccac aaacaatacc gatgatgata
      121 gttgctgaat gtaattatta ttgcctaagt gctttataca ttgtttgcaa caagccaggc
      181 ttgaaaatac ttataaatgg ttataaaaaa tccttataaa acattaattc atagatacat
      241 taggaataac aattaaatcc gtgagtgccg tagcgcagcc tttcatcagg actttcgaaa
      301 aatccgaaat catgcatcgg aatttactga tttaattatt ttaatcctaa tttattttga
      361 aaaaggcatt gggattt
//
LOCUS       DAJTUN010000411          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTUN010000411 DAJTUN010000000
VERSION     DAJTUN010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.417"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..311)
                     /locus_tag="OO470_004199"
     CDS             complement(<1..311)
                     /locus_tag="OO470_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6253241.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTUN010000412          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTUN010000412 DAJTUN010000000
VERSION     DAJTUN010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.418"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            2..361
                     /locus_tag="OO470_004200"
     CDS             2..361
                     /locus_tag="OO470_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253242.1"
                     /translation="MPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIEL
                     FRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQ
                     EGLVAPSQEPQDVKKAV"
BASE COUNT          134 a           70 c           69 g           97 t
ORIGIN      
        1 catgccaatg gttgagcata aaaataatca agagaataaa aattctcaac ctgaccgcag
       61 cacattgaaa actaaactga tcgcaattgt cgaattgtta gaggacaagg aactgcattc
      121 tgcgattgag ttgttcagaa agaaaggatt tgaagctctt atgcctgaga ttttttcttc
      181 cgatgattcc atccaggctg atttgcaagg tatttcgcag caaaccttac aaacagccaa
      241 aatgcttgaa tcattaccaa cagatactcg taaaaagatt ttgtcaaagt atggtattta
      301 tgaacaggaa ggtcttgtag ctccttcaca agaaccacaa gatgtaaaaa aagccgtata
      361 acaaccatga
//
LOCUS       DAJTUN010000413          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTUN010000413 DAJTUN010000000
VERSION     DAJTUN010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.419"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            21..320
                     /locus_tag="OO470_004201"
     CDS             21..320
                     /locus_tag="OO470_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6253243.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
BASE COUNT          127 a           86 c           86 g           69 t
ORIGIN      
        1 cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc agtggcaaca
       61 ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc atggtaacga
      121 ttaaaggcgt ggcagaacgg cgcatcacct accgccgtga aggttacgaa tatgattgcg
      181 tgatgcctgt ttatcaattc cggcgtgatt tcacgctggt acaggcagcg ccccgcagta
      241 aaccaaccag cagggagaaa gcacgcgcca atattcagga aataaaaaag atgcttaacg
      301 tattcagggg taaaaaatga aactggcacc gaacgtaaaa aaacagccac gcggaataaa
      361 acacaaag
//
LOCUS       DAJTUN010000414          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTUN010000414 DAJTUN010000000
VERSION     DAJTUN010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.420"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(14..88)
                     /locus_tag="OO470_004202"
     tRNA            complement(14..88)
                     /locus_tag="OO470_004202"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Gln,seq:ttg)
     gene            complement(123..197)
                     /locus_tag="OO470_004203"
     tRNA            complement(123..197)
                     /locus_tag="OO470_004203"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Gln,seq:ttg)
     gene            complement(221..305)
                     /locus_tag="OO470_004204"
     tRNA            complement(221..305)
                     /locus_tag="OO470_004204"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Leu,seq:tag)
BASE COUNT           89 a           98 c           99 g           80 t
ORIGIN      
        1 attatttctt cgatggctgg agtacctgga ttcgaaccag ggaatgccgg tatcaaaaac
       61 cggtgcctta ccgcttggcg ataccccaat aaccgggcgg tgaaccgctt actcgaagaa
      121 gatggctggg gtacctggat tcgaaccagg gaatgccggt atcaaaaacc ggtgccttac
      181 cgcttggcga taccccatcc gtacaacgct ttctggtgaa tggtgcggga ggcgagactt
      241 gaactcgcac accttgcggc gccagaacct aaatctggtg cgtctaccaa tttcgccact
      301 cccgcaaaaa agatggtggc tacgacggga ttcgaacctg tgaccccatc attatgagtg
      361 atgtgc
//
LOCUS       DAJTUN010000415          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTUN010000415 DAJTUN010000000
VERSION     DAJTUN010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.421"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(91..>365)
                     /locus_tag="OO470_004205"
     CDS             complement(91..>365)
                     /locus_tag="OO470_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253244.1"
                     /translation="LNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLNDAVE
                     ELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT           95 a           89 c           75 g          106 t
ORIGIN      
        1 cagatgacaa tgcttttatc ccttttcact atcataccct ttatccttgc tgaatcgaag
       61 cagcagcaag atgattctga agttcagaaa ttatttgcgc aaggcccggc aaatcagatt
      121 cgatagcaac tcaagctgat gtgccgtttc catgttcagc cacacataac catagattgg
      181 tgtttccacg actttcaggt catggtggtt atcgagcaac tgacgcagct cttccacagc
      241 atcgttcaat ttttccgtat tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg
      301 aacaaggctc agcagtattt gctgcatcat gtgctgggta tcacaaagtt tttgcgcgtt
      361 caata
//
LOCUS       DAJTUN010000416          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTUN010000416 DAJTUN010000000
VERSION     DAJTUN010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.422"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>365)
                     /locus_tag="OO470_004206"
     CDS             complement(<1..>365)
                     /locus_tag="OO470_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253245.1"
                     /translation="VMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDEPWG
                     GVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDG
                     NGDAFLTAWAWYADWQASQA"
BASE COUNT           75 a          119 c           98 g           73 t
ORIGIN      
        1 gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc gtcaccattg
       61 ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat cagccgcaga
      121 cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg atcggcaagc
      181 gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac cccaccccac
      241 ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt ttcactactt
      301 tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc accgccgagc
      361 atgac
//
LOCUS       DAJTUN010000417          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTUN010000417 DAJTUN010000000
VERSION     DAJTUN010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.423"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>350
                     /locus_tag="OO470_004207"
     CDS             <1..>350
                     /locus_tag="OO470_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253246.1"
                     /translation="NYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVNAG
                     DSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALP
                     PCSGQAFPADNNGYA"
BASE COUNT           92 a           84 c           89 g           85 t
ORIGIN      
        1 aattatcaat tatatgtccg taattacagt cgtaacacca actacgagat cgttgctacg
       61 gctgatggcc tggatgtgct gaacggcaag caaggttcgc tgaataacaa cggctatatt
      121 gtgaatgccg gagattcgtt agtgattaaa ggattccgca aagataaaca tactgaagcg
      181 gcttttcagt tcgccaacgt tgctgattcc tacgcagcca attcggcgca aggcgatgtg
      241 cgtaataccg gggtaatcgg ctttgccgcc tttgaattac aaggcccggc gcaaaacgcg
      301 ctgccgccgt gctctggaca agcctttccg gcagataaca atggctatgc
//
LOCUS       DAJTUN010000418          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTUN010000418 DAJTUN010000000
VERSION     DAJTUN010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.424"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..282)
                     /locus_tag="OO470_004208"
     CDS             complement(<1..282)
                     /locus_tag="OO470_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6253247.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMK"
BASE COUNT           67 a           91 c           80 g          103 t
ORIGIN      
        1 tttcattctg cacaaatcgc cgactctctg ccctgtaacg acagccaaat ccattgctag
       61 cctcagccag attgggagag gttcggctgc atggtaaatc gcgacatact cattagctgt
      121 cagccttgag cgccttactt ctgactttgc tgtgcgggtt gctgttaccg gattcgttgc
      181 tacatgcccc tcggctattg cctcacgaaa aacgtcaaca agggttgacc tgattaattt
      241 tgcggaagcc gctttacctt ctgctacgta ggtgtttagc attgctgcca cctctttcgt
      301 tgatatgtca gcgagcggtt tgtccggcaa ttttcttcgg a
//
LOCUS       DAJTUN010000419          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTUN010000419 DAJTUN010000000
VERSION     DAJTUN010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.425"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            144..>340
                     /locus_tag="OO470_004209"
     CDS             144..>340
                     /locus_tag="OO470_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253248.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLA"
BASE COUNT          100 a           84 c           63 g           93 t
ORIGIN      
        1 ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact catagctaac
       61 cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta ggaaccatct
      121 gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt catgcatcac
      181 gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg
      241 ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa
      301 accgggaatt atttcaggtc tggttacaaa aattagcgca
//
LOCUS       DAJTUN010000420          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTUN010000420 DAJTUN010000000
VERSION     DAJTUN010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.426"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(189..>339)
                     /locus_tag="OO470_004210"
     CDS             complement(189..>339)
                     /locus_tag="OO470_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR6253249.1"
                     /translation="ADLASMEQISLVKSWLREAGAHNVLVTSAVNNNGVTELFALLHT
                     EEGCC"
BASE COUNT           99 a           86 c           61 g           93 t
ORIGIN      
        1 caaaaatgcc gagctctcca aagaacgccg cgatatctcc aaaaagctga aagctatggg
       61 cgaaatgaaa aacggcgaag cgaagtaatt cccgttttat tcaatgaggg ttgcccggca
      121 accctcattg ctcattgatt cttatctgtg tatcaccgtc atcattctca tctgagaacc
      181 aatcgaaatt aacaacagcc ttcttctgta tgcagcaagg caaaaagttc tgtaactcca
      241 ttgttattaa ctgcactggt tactaacacg ttgtgcgctc cagcttcccg taaccaactt
      301 ttcaccaaag atatttgttc catgctggct aaatctgct
//
LOCUS       DAJTUN010000421          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTUN010000421 DAJTUN010000000
VERSION     DAJTUN010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.427"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..183)
                     /locus_tag="OO470_004211"
     CDS             complement(<1..183)
                     /locus_tag="OO470_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6253250.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLG"
BASE COUNT           77 a           91 c           89 g           80 t
ORIGIN      
        1 acccaggtgc tcacgtccgg cggcgatcgc gcccgacgtc acaataacaa tccgatgccc
       61 ggcggcatgt aactgcgcgc actggcgaac aagttcaacg atatgggcac ggttcagacg
      121 gcgcgatccg cctgttagca cactggtgcc gagttttacc accagcgtct ggctgtcact
      181 catgattctc tgccattcaa ttttaggaaa aatgatatca aacgaacgtt ttagcaggac
      241 tgtcgtcggt tgccaaccat ctgcgagcaa agcatggcgt tttgttgcgc gggatcagca
      301 agcgtagcgg cagttgttta cgcttttatt acagatt
//
LOCUS       DAJTUN010000422          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTUN010000422 DAJTUN010000000
VERSION     DAJTUN010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.428"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>336
                     /locus_tag="OO470_004212"
     CDS             <1..>336
                     /locus_tag="OO470_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6253251.1"
                     /translation="KEFTFNKIRQPNRNRLLWSSNLNVDGMKTGTTAGAGYNLVASAT
                     QGDMRLISVVLGAKTDRIRFNESEKLLTWGFRFFETVTPIKPDATFVTQRVWFGDKIE
                     VNLGAGEAGS"
BASE COUNT           82 a           72 c           94 g           88 t
ORIGIN      
        1 gaaagagttc accttcaaca aaattcgtca gcctaaccgt aaccgtctgc tgtggagcag
       61 taatctgaat gttgatggca tgaagacagg aaccacggca ggtgcgggat ataatctggt
      121 tgcttcggct acccagggtg atatgcgttt aatctccgta gtgctggggg cgaaaactga
      181 ccgtatccgt tttaatgagt ctgagaaatt attgacctgg ggtttccgct tctttgaaac
      241 tgtgacgcca attaaacctg atgccacctt tgtgactcag cgcgtctggt ttggtgataa
      301 gatcgaagtg aatctcgggg caggcgaagc gggctc
//
LOCUS       DAJTUN010000423          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTUN010000423 DAJTUN010000000
VERSION     DAJTUN010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.429"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT           80 a           70 c           87 g           96 t
ORIGIN      
        1 ttgttagtct ttgcatgagc aagcggactg acacatttct ctttgttctc attcagaaaa
       61 tctcatcagt cggcgttcag accggtgttg tgctttatgg atacgttacg ctcctgatga
      121 cgtcatttga cgttcaacag catcacgggg ccgcacgaca tttcacgtca gttagtgcta
      181 tagctcagga acaaattttc ccaaattggg atatgctcgc aaattgctgg tgatgtgggg
      241 agaatctggt tgagttcggt agaattgatt tggaggcaga acgcttaaat cgtggcgtcc
      301 tgaaacgaaa aacggacctc cgtggaggtc cgt
//
LOCUS       DAJTUN010000424          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTUN010000424 DAJTUN010000000
VERSION     DAJTUN010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.430"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            175..>327
                     /locus_tag="OO470_004213"
     CDS             175..>327
                     /locus_tag="OO470_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6253252.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLA"
BASE COUNT          110 a           63 c           74 g           80 t
ORIGIN      
        1 ataccggcta ccgctatcgg gaaaaacgat accgcagaaa aatgctcaat tcaagagcta
       61 tgctcatttt tattttaacc agtgcaaatc aaagaattct gtaaaattct ttatataaca
      121 gagagattgg gcatgacagg tgaaaaagaa agcaaaaatg cagagcgcat ttttatgccc
      181 ccggaaataa tgcgtttcaa ggaacgcctt gaagaggcaa tgaatggaga atcaagccgg
      241 gcgtttgcag ctaagtgcgg actttctgat ggggttatca gaaactacct gtctggcaag
      301 acatacccgt ctttagatcg attagca
//
LOCUS       DAJTUN010000425          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTUN010000425 DAJTUN010000000
VERSION     DAJTUN010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.431"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            51..>325
                     /locus_tag="OO470_004214"
     CDS             51..>325
                     /locus_tag="OO470_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6253253.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIEST"
BASE COUNT          117 a           58 c           73 g           77 t
ORIGIN      
        1 aattgcatct gcgggccgta tggctcgcag tgatttcact ggagaaaaat atgaatatga
       61 agaccattga agatgtattt attcaactgc tttcagatac ctacagcgca gaaaaacaat
      121 taacccgggc actggcaaaa ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt
      181 ttcatgcgca cctcgaggaa actcatggac agattgaacg aattgatcaa gttgtggaat
      241 cagaatcaaa tctgaaaatt aagcgcatga aatgtgtggc aatggaaggt cttattgaag
      301 aagctaatga ggtcatcgaa agtac
//
LOCUS       DAJTUN010000426          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTUN010000426 DAJTUN010000000
VERSION     DAJTUN010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.432"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT           87 a           77 c           90 g           71 t
ORIGIN      
        1 taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt
       61 gcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct
      121 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg
      181 acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg
      241 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
      301 cctcccgagg aaggggcctt ataga
//
LOCUS       DAJTUN010000427          324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTUN010000427 DAJTUN010000000
VERSION     DAJTUN010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.433"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>324
                     /locus_tag="OO470_004215"
     CDS             <1..>324
                     /locus_tag="OO470_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253254.1"
                     /translation="RMHDAFPNRPASSITVKQWVDLLTEEEKDNPRRARQVLSQLRSA
                     ISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIERSRASTSNKLL
                     HQMLM"
BASE COUNT           85 a           76 c           85 g           78 t
ORIGIN      
        1 agcgcatgca tgacgccttc cctaatcgtc cggcatcttc tatcacggtt aagcaatggg
       61 ttgacctgct taccgaagaa gaaaaagata atccacgccg agcaaggcag gtgctaagtc
      121 aactaagatc agcaattagt tggtgcatgc ggcgtcagtt gatagatagt tgcgcaatta
      181 tgagcatcca gccaagggac ttcggctccc gcgctgaggt aggggatcgg gtactgtcgt
      241 atcacgaact ggctaagatt tggcttgcta ttgaaagaag ccgtgcgtct acgtcaaata
      301 agctccttca tcagatgctt atgc
//
LOCUS       DAJTUN010000428          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTUN010000428 DAJTUN010000000
VERSION     DAJTUN010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.434"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..227
                     /locus_tag="OO470_004216"
     CDS             <1..227
                     /locus_tag="OO470_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6253255.1"
                     /translation="TNGSVRYCQYTSVYEWQKDVDLHQVLWSRSRLGERPKGQGITGA
                     DHFWFGHTPLRHRVDIGNLHYIDTGAVFGG"
     gene            complement(252..>321)
                     /locus_tag="OO470_004217"
     CDS             complement(252..>321)
                     /locus_tag="OO470_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR6253256.1"
                     /translation="GCFPIEDFIDGFWRPAQEYGDF"
BASE COUNT           87 a           72 c           77 g           85 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcagt ttatgaatgg caaaaggacg
       61 ttgatttgca tcaggtcttg tggagccgct cgcgattagg tgaacgccca aaagggcagg
      121 gaattacagg tgctgatcat ttctggtttg gtcatacacc gttgcgacat cgcgtggata
      181 ttggcaacct gcattatatt gataccggtg ctgtctttgg gggctaactg actcttgtgc
      241 aactgcaata attaaaaatc accatactcc tgtgctggtc gccagaaacc atctataaaa
      301 tcctcaatcg gaaaacaacc g
//
LOCUS       DAJTUN010000429          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTUN010000429 DAJTUN010000000
VERSION     DAJTUN010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.435"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT           93 a           60 c           83 g           85 t
ORIGIN      
        1 tcaaaaacac catcatacac taaatcagta agttggcagc atcacctcga atggcaacat
       61 gcgcgggcct gaggccaaag aaatgtaaaa cactgttgtt atttgtaaca acggaaacat
      121 ttacggaaat tatttgtctt tcttcgtgat caaaaatgct gtgtcttgtt ggattggagt
      181 gatgagggct gcgtttagga aacgccctat attgtacggt tgcattatcg cggtaggggt
      241 aatgcacgtg tcagtatgat gagctgacca cactcacgtg ggtggactgg tataacaatc
      301 gacgattgct ggaaaggcag g
//
LOCUS       DAJTUN010000430          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTUN010000430 DAJTUN010000000
VERSION     DAJTUN010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.436"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>321
                     /locus_tag="OO470_004218"
     CDS             <1..>321
                     /locus_tag="OO470_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6253257.1"
                     /translation="LIIDNWETVGPVVKKVWQEVDQVVRAMGGWEQAVKTIATVSALY
                     IGVKAVASIRAATVAQNQWTTAAGKTGLKLKGLGKISLIGGLLELGMMAQEFEKEHPW
                     LVKN"
BASE COUNT           95 a           53 c          101 g           72 t
ORIGIN      
        1 tctcataatt gataactggg aaacggtcgg tccggttgtg aaaaaagtct ggcaggaggt
       61 tgatcaggtt gtcagagcaa tggggggatg ggaacaggca gtaaaaacaa tagcaacggt
      121 ttctgccctg tatattggtg tgaaggcggt ggcaagtatt cgtgcggcaa cagtcgccca
      181 gaatcaatgg acaacggcgg caggaaaaac agggctgaaa ttaaagggcc tcggaaagat
      241 cagtctgatt ggtggattgc ttgaactggg catgatggcg caggagtttg aaaaagaaca
      301 tccctggctt gttaaaaatt t
//
LOCUS       DAJTUN010000431          311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTUN010000431 DAJTUN010000000
VERSION     DAJTUN010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.437"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(<1..>311)
                     /locus_tag="OO470_004219"
     CDS             complement(<1..>311)
                     /locus_tag="OO470_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6253258.1"
                     /translation="KQHVCLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQL
                     CLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFID
                     F"
BASE COUNT           79 a           84 c           68 g           80 t
ORIGIN      
        1 ggaaatcgat aaaagcccgg atgcgcgtac ttaccgcacg gtcgctgtaa tagacggcac
       61 tgaatggcat ttccactggc aacactttat ctgccattaa ctccaccaat tctccgcgag
      121 cgatttcttt gtcgatcatg tagtcggaca aacacgcaat cccgttgcca ctcaggcaaa
      181 gctgtttcag tgtttcccca ctattggatg acaaaccgta cttcacctca tgtaattgtc
      241 catcactaca ggctatcggc caggtattga gggaagcggg ttcagtgaat cccaggcaaa
      301 catgttgctt t
//
LOCUS       DAJTUN010000432          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTUN010000432 DAJTUN010000000
VERSION     DAJTUN010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.438"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT           92 a           61 c           49 g          108 t
ORIGIN      
        1 gtcgtatcgt caatatggtg gcgctggccg tggttgaagc gcaaacccaa ccgctgtaat
       61 tcattcttaa ctctcacgct taccctgaat attcagggta agcggttttc ctgaaatata
      121 ttagcaaagc ttattactga gtttgcgaat aataaaaaaa tcagtatata aaatatctcg
      181 atattatttt atttatattc atgcgtcgca tatgaaagtt tattcataac agtaaatatc
      241 tctccattct tattgatcta cctcaccttt taaacgcgct tgccgaattt tgttatttac
      301 tctgacgaaa
//
LOCUS       DAJTUN010000433          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTUN010000433 DAJTUN010000000
VERSION     DAJTUN010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.439"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..>310
                     /locus_tag="OO470_004220"
     CDS             <1..>310
                     /locus_tag="OO470_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253259.1"
                     /translation="VPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVENVVD
                     VYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGNTTF
                     T"
BASE COUNT           82 a           79 c           85 g           64 t
ORIGIN      
        1 tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac gtaattcagt
       61 tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct ccacggtaga
      121 aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa aagccggagt
      181 cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg gcagcccggc
      241 agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga atggcaacac
      301 aacgttcact
//
LOCUS       DAJTUN010000434          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTUN010000434 DAJTUN010000000
VERSION     DAJTUN010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.440"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT          122 a           43 c           59 g           81 t
ORIGIN      
        1 aactatattc tcaaaatata gtagaaacga ggtatgtttc tggtggaaag gacagtggga
       61 ttaaaaagta agaattgata aaaaaaacgc cagcaacaca tagtgccggc ggagggaata
      121 ccccatggag aaaatgtgat gcctagagca tcatgaatat ataattaaaa atagttagcg
      181 ttgtcaatac tttcacaaaa ataattttcg tagtatagaa agatattttt atgcatgacc
      241 tacctgaatt tgctccgggt agaggttata aataaaaatt gaatcacgac aaacacaata
      301 ttcat
//
LOCUS       DAJTUN010000435          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTUN010000435 DAJTUN010000000
VERSION     DAJTUN010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.441"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            complement(41..>304)
                     /locus_tag="OO470_004221"
     CDS             complement(41..>304)
                     /locus_tag="OO470_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6253260.1"
                     /translation="IGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVT
                     LRISVGVAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT           71 a           92 c           69 g           72 t
ORIGIN      
        1 tcagaaaaac tcagcaaatc ctgatgactt tcgccggacg tcaggccgcc acttcggtgc
       61 ggttacgtcc ggctttcttt gctttgtaaa gcgccaaatc tgccgatttc aaccactcac
      121 gatagtgact catttgtggg ttcagcggcg caacccccac actaatccgt aaagttacct
      181 gtggcgtatt cggcaagcgt aacgtgttta gcccttcacg cacccgtaac atggcggtaa
      241 tggcgctctc agctggcgta ccggacatga ttactgcaaa ctcatcgccg ccaaaccgac
      301 caat
//
LOCUS       DAJTUN010000436          301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTUN010000436 DAJTUN010000000
VERSION     DAJTUN010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.442"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            1..178
                     /locus_tag="OO470_004222"
                     /pseudo
     CDS             1..178
                     /locus_tag="OO470_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(165..>301)
                     /locus_tag="OO470_004223"
     CDS             complement(165..>301)
                     /locus_tag="OO470_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6253261.1"
                     /translation="VYANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN
                     "
BASE COUNT           67 a           77 c           78 g           79 t
ORIGIN      
        1 cgccaacgga tgattgccga gatatttact tccgggtatg tataagcgcc tgattaccaa
       61 tgtgcgcagc gtgaaggtcg gttaccaggc gttactgtgg tctttccgct tatggcaatg
      121 gcgcgacaaa acgcggtcgc accatcgcat tacgcgtagc gcctttaatt tgcgctagcg
      181 ttaaacttcg ccgcttcggg ctgtttgccc cagccttgcc actgatgctg gaagtaaacg
      241 atcagcgtgc tctggctgat gctttcgctt aatacttcaa agaagcgatt agcataaaca
      301 g
//
LOCUS       DAJTUN010000437          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTUN010000437 DAJTUN010000000
VERSION     DAJTUN010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.denovo.443"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
BASE COUNT           71 a           74 c           79 g           76 t
ORIGIN      
        1 tgctgactcc ccgattgttg atgagcgccg gacctcgcgc aacgctttcc cagaggcagt
       61 tttgccttcc ccgagcggtc acgcaaaacg ttgcaacgac ctgatcattt gcagttttta
      121 gcggaacgca gatataaaaa atccccggct ttttgtgcca gggacgttca tagctgaaaa
      181 tttattttac gttttcggcg acgggagttg ttgagattaa gggtgaagtc aatgccgcca
      241 gtgaaccgcc tgaaacgcca atttcattga gcaaggagtc aatcagcgga gcctgtgtgc
//
LOCUS       DAJTUN010000438         6893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.guided.161, whole genome shotgun sequence.
ACCESSION   DAJTUN010000438 DAJTUN010000000
VERSION     DAJTUN010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.guided.161"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            385..858
                     /locus_tag="OO470_004224"
     CDS             385..858
                     /locus_tag="OO470_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6253262.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            953..1477
                     /gene="sat2"
                     /locus_tag="OO470_004225"
     CDS             953..1477
                     /gene="sat2"
                     /locus_tag="OO470_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6253263.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1535..2323
                     /gene="aadA1"
                     /locus_tag="OO470_004226"
     CDS             1535..2323
                     /gene="aadA1"
                     /locus_tag="OO470_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6253264.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2399..2896
                     /locus_tag="OO470_004227"
     CDS             2399..2896
                     /locus_tag="OO470_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253265.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            2957..3328
                     /locus_tag="OO470_004228"
     CDS             2957..3328
                     /locus_tag="OO470_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253266.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            3738..4115
                     /locus_tag="OO470_004229"
     CDS             3738..4115
                     /locus_tag="OO470_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6253267.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            4138..4290
                     /locus_tag="OO470_004230"
                     /pseudo
     CDS             4138..4290
                     /locus_tag="OO470_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4346..5962)
                     /locus_tag="OO470_004231"
     CDS             complement(4346..5962)
                     /locus_tag="OO470_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6253268.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(5963..>6893)
                     /locus_tag="OO470_004232"
     CDS             complement(5963..>6893)
                     /locus_tag="OO470_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6253269.1"
                     /translation="TLTRWPVFVDHFTPSLEQWTLFYQRLAQDLGLTKSKHIRHDLVA
                     ERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKAFSYLQHSIVWQALLPKLTVI
                     EALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQLVHKYQGIKAARQSLEGGV
                     LYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIAVRQLLRIIKRLDSSLDHP
                     RATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDYQIRRISQAFIKLKQEDV
                     ELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
BASE COUNT         1868 a         1645 c         1518 g         1862 t
ORIGIN      
        1 cctttttgtc gcatatccgt gcgtatagaa tttaaaaatg gactgttaga cataaaacgc
       61 tccttgtctt gcaactgtct gcctatacag cctattctag ctgggattta aaaaagtgcc
      121 tgttttttac gcctagagat gcttgtttac cggtagagtt ttaatttaat gctaaataaa
      181 ttaaaatgtt atgagttctt tgggtgagat aatgtgcatc gtgcaagcag gatagacggc
      241 atgcacgatt tgtaataaca gagtgtcttg tatttttaaa gaaagtctat ttaatacaag
      301 tgattatatt aattaacggt aagcatcagc gggtgacaaa acgagcatgc ttactaataa
      361 aatgttaacc tctgaggaag aattgtgaaa ctatcactaa tggtagctat atcgaagaat
      421 ggagttatcg ggaatggccc tgatattcca tggagtgcca aaggtgaaca gctcctgttt
      481 aaagctatta cctataacca atggctgttg gttggacgca agacttttga atcaatggga
      541 gcattaccca accgaaagta tgcggtcgta acacgttcaa gttttacatc tgacaatgag
      601 aacgtattga tctttccatc aattaaagat gctttaacca acctaaagaa aataacggat
      661 catgtcattg tttcaggtgg tggggagata tacaaaagcc tgatcgatca agtagataca
      721 ctacatatat ctacaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc
      781 agcaatttta ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa
      841 atctggcaaa agggttaaca agtggcagca acggattcgc aaacctgtca cgccttttgt
      901 accaaaagcc gcgccaggtt tgcgatccgc tgtgccaggc gttaggcgtc atatgaagat
      961 ttcggtgatc cctgagcagg tggcggaaac attggatgct gagaaccatt tcattgttcg
     1021 tgaagtgttc gatgtgcacc tatccgacca aggctttgaa ctatctacca gaagtgtgag
     1081 cccctaccgg aaggattaca tctcggatga tgactctgat gaagactctg cttgctatgg
     1141 cgcattcatc gaccaagagc ttgtcgggaa gattgaactc aactcaacat ggaacgatct
     1201 agcctctatc gaacacattg ttgtgtcgca cacgcaccga ggcaaaggag tcgcgcacag
     1261 tctcatcgaa tttgcgaaaa agtgggcact aagcagacag ctccttggca tacgattaga
     1321 gacacaaacg aacaatgtac ctgcctgcaa tttgtacgca aaatgtggct ttactctcgg
     1381 cggcattgac ctgttcacgt ataaaactag acctcaagtc tcgaacgaaa cagcgatgta
     1441 ctggtactgg ttctcgggag cacaggatga cgcctaacaa ttcattcaag ccgacaccgc
     1501 ttcgcggcgc ggcttaattc aggagttaaa catcatgagg gaagcggtga tcgccgaagt
     1561 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     1621 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     1681 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     1741 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     1801 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     1861 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     1921 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     1981 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     2041 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     2101 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     2161 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctaggca
     2221 ggcttatctt ggacaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     2281 tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc
     2341 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agagagctgg ggaagactat
     2401 gcgcgatctg ttgaaggtgg ttctaagcct cgtacttgcg atggcatcgg ggcaggcact
     2461 tgctgacctg ccaattgttt tagtggatga agctcgtctt ccctatgact actccccatc
     2521 caactacgac atttctccaa gcaactacga caactccata agcaattacg acaatagtcc
     2581 atcaaattac gacaactctg agagcaacta cgataatagt tcatccaatt acgacaatag
     2641 tcgcaacgga aatcgtaggc ttatatatag cgcaaatggg tctcgcactt tcgccggcta
     2701 ctacgtcatt gccaacaatg ggacaacgaa cttcttttcc acatctggca aaaggatgtt
     2761 ctacacccca aaaggggggc gcggcgtcta tggcggcaaa gatgggagct tctgcggggc
     2821 attggtcgtc ataaatggcc aattttcgct tgccctgaca gataacggcc tgaagatcat
     2881 gtatctaagc aactagcctg ctctctaata aaatgttagg cctcaacatc tagtcgcaag
     2941 ctgaggggaa ccactagtgt catacgaacc tccaagagac ggttacacaa acgggtacat
     3001 tgttgatgtc atgtatgaca atcgcccaag taagtatcca gctgtgttca gaacgtacgt
     3061 ccgaattcca gactatgtcg aactagataa cgcggaggag ctggccgacg caatcattcg
     3121 agaggttgag ttaaaggagg catggctcac aagggtcttg gggaactcag ccgatcctga
     3181 cgcttctgtg ggcaagtggg accggcgcct agcgcagtgc ggtggtattg atgagatgtc
     3241 aagtggggtg caggcaacat acttcagtcc aaacggtcag ctggtcggct tagtggcata
     3301 cattgatgcc gaatggatgg atgaatagct ctgacgttac gattcaggtg agacaaccag
     3361 atgggcattg cgtacaagct cagttgccac cgatgtggct acgagtctga tcttctttac
     3421 ctaggccaag gtatggcgat gcttcctgag caaatagtcg gaacgtgcac atgctgcgac
     3481 aatctaacga cgatttctat aaatgaaagc tacggagtat gctcactttg tggggctaag
     3541 gatcgtgtga tatttacaag ctatcagcat gaggcaaggc caagaaatcc ctgctatcta
     3601 gagagaagtt tcaaatatca atgcccaaga tgccatgcct tcagtatgga gcctccggat
     3661 ctaccggaga tgctttggga ctaatgctcc gctttgcctc gggtatccgt tcgacctatg
     3721 tttaagagga aacgcacgtg tctgccgcca aagctcttgc ctatgcactt tgtgacaagt
     3781 tggaaagcaa aaaagttgat gaagtcgtac gaaatcttgt ttctagcggc tggaatatta
     3841 gggaagtggc ctgggaagct tcgtcctttg atcatgaaat gccctacttt gacacggggc
     3901 gggacttcga tattggattt cctgacacgc tgtcatccaa gattgagttt ttgaccgcga
     3961 atggatcaat cgtccaagca cgtgctcgcc tgcacttcaa gcaggctcta atttttgcca
     4021 aggcgtccaa acatgtcgcc gcgctcaaga actcattgga ttactactat ggatcgtctg
     4081 taatcccccc tcaaaaccac ggaggctatt tgtagaaaat tccattaata tgaactcaag
     4141 gtgttcccta cggacgaatc agcgatgaag gtggtgtacc tggctatcca ggcggcgacc
     4201 aagaaatgga cgatgccgat ccgcaactgg aagccggcca tgaaccgttt tatgatcgaa
     4261 tttggtgacc gtttaaatgg ccacctttaa ctgaccggca aaagcactta cacaaaaggg
     4321 tttacaggct ctttcagtca ttcctttaat gcgtaaattg ctctctaacc actctctcag
     4381 cccacccttc catatcctct ctcgatactt gcagctcccc caaccccttt ggatgattca
     4441 cccctctatg cccgtcttgt ccatataatt gatcaaacaa actatttggc caattcaatg
     4501 agctcttcac cactccacgc cgtatctttt caaaatcatt ccgccatgtt tcgctatcaa
     4561 cgccactcaa cactttggta gaaagcattt tcaccccatc cgatgcatcc acctctagca
     4621 atacaaattc actgccatta cgttgcaccc caacggcttt aatcactctt ggtgagccat
     4681 ctttacacag atgtaatcgg ctacgcccaa cttttggcaa aaccaaggtt tcttcaaaaa
     4741 gcacccgaca tgcaaattta gtttttagaa ttgaaagtag ctcatcaaaa gcagcaaatc
     4801 gattagcaaa aatagagttg taattggttg catcctgctt cccacccaca tctgccgctg
     4861 ctaggacacc acctaagtgt ggctcatcag tactaaccaa attactattt tcacttgttg
     4921 ttgcttcttc tttttctaaa atagtttggc tactttttct tgccctgcga gatttttcaa
     4981 ttcgactagg tctactaaaa cttatccatg tcggctcagc ctctatgact cgtgtgtctg
     5041 tgtctatatt ggctgtttcc tcatcgtcca actgtagatg ctcatcatca acaactgact
     5101 ctgttgaaac cgctatgtgc tgcgtactac catcacctgc ctccttttcc tgaaaagagg
     5161 catggctaat agctgttgtg ctaggaagca tagcgttgat ttctaaacct actatttcct
     5221 caactaaata atccttatcc tcgttagaag aacgtccttt tacatgtaat ttccaaccct
     5281 gcatgggcgg agggtcaaag ctaaagcacc agctttcaac gccgttctta atttctctat
     5341 tttgttgata gtgcctaaaa atactttcat acgaatccat aacatcttga tccgaaaaca
     5401 accaaaccaa aagctgcact acggccgact gctctaacgc ccctttagga aacgatgaac
     5461 tgggtaagat ccttatctct aaatgatctc gctcaacctc atactgaacg tcgaactctt
     5521 gctgtaaagc ggtactgctc aaacagcttc gacagaaata ggagttaatt aaaaataatg
     5581 accgagctag ctctaactgt gggatgtgat aacgagtctt gctatatggt gtttcagctt
     5641 cgtaaataaa tgaactctgc gctccatcct tgttgcgtac ggaagcaaag gacgagagat
     5701 taggaaaatc gattattttg gctcgttgaa gatccgatgc ttcaaactca atcacacgat
     5761 ccgccttatt tatttgcttt gttgtactgt taaccacctt agctcgactt aataaaggca
     5821 aatgagtaaa tcgagtccat ttccgttctt gcattggatt aaaccaaaca aaaatacgcc
     5881 actccttatg acccgagtta cggaataaat gaccaatatg tacaacctgc acattgtcat
     5941 taaatgtagc tagcctaacc actcactctt ccccataaac catttccaag aatctttgtg
     6001 cttcctcagt tatccgctct ttagataacg ttgcacttct taataatcgc cagcgcctaa
     6061 gctcaacatc ttcctgttta agcttaataa aagcttggct aatccgtcta atttgataat
     6121 cttccacact ctctgagtaa cgctttaagc aaagcgctac caaaggcagt ttctgtagat
     6181 tttttgcaag agaggttccg ttaggagttt gctttaacag ccagctcgat gtcgctcttg
     6241 ggtgatcaag gctactatct agacgcttta tgattcttaa tagttgtcgt acagcaattc
     6301 gatctctttg gttccaatca actctagggg cgggtgccag acgctcttgt tgatgctgtt
     6361 gattccagtg aactagccaa tccctgtcat gtcggtaaag ccaagcgtat agcaccccac
     6421 cctctaaaga ctgtcttgcc gccttaattc cttggtattt atgcactagt tgctgccagt
     6481 ctttatgctt aacagataaa tcttcagagt taggttgcac agactggcta acaggtcttg
     6541 tcgttataga gtgctcagta agagcacttg cctgctgtag cgcttctata accgttagtt
     6601 ttggcaataa ggcttgccac acaatactat gctgtaaata actaaaggct tttctatgct
     6661 tacggaatat acttttcagc caacacgtgt ccttgttctc tgccaacttt aaatccagtt
     6721 tctctagtgc ctcatcacta aaagtctgcc tcactctctc cgccaccaag tcatgacgaa
     6781 tgtgcttgct tttggttagc cctagatcct gcgctaagcg ctgataaaac agcgtccact
     6841 gctcaaggct tggggtgaag tggtcaacaa aaactggcca ccgagttaga gtt
//
LOCUS       DAJTUN010000439         5684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.guided.176, whole genome shotgun sequence.
ACCESSION   DAJTUN010000439 DAJTUN010000000
VERSION     DAJTUN010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.guided.176"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            <1..777
                     /locus_tag="OO470_004233"
     CDS             <1..777
                     /locus_tag="OO470_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6253270.1"
                     /translation="VKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHG
                     ANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGG
                     ENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLL
                     YTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTT
                     RLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            770..1504
                     /gene="frdB"
                     /locus_tag="OO470_004234"
     CDS             770..1504
                     /gene="frdB"
                     /locus_tag="OO470_004234"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6253271.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1515..1910
                     /gene="frdC"
                     /locus_tag="OO470_004235"
     CDS             1515..1910
                     /gene="frdC"
                     /locus_tag="OO470_004235"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6253272.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1921..2280
                     /gene="frdD"
                     /locus_tag="OO470_004236"
     CDS             1921..2280
                     /gene="frdD"
                     /locus_tag="OO470_004236"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6253273.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2343..3476
                     /locus_tag="OO470_004237"
     CDS             2343..3476
                     /locus_tag="OO470_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6253274.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3565..4098
                     /gene="blc"
                     /locus_tag="OO470_004238"
     CDS             3565..4098
                     /gene="blc"
                     /locus_tag="OO470_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6253275.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4095..4412)
                     /gene="sugE"
                     /locus_tag="OO470_004239"
     CDS             complement(4095..4412)
                     /gene="sugE"
                     /locus_tag="OO470_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6253276.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4588..4734)
                     /gene="ecnB"
                     /locus_tag="OO470_004240"
     CDS             complement(4588..4734)
                     /gene="ecnB"
                     /locus_tag="OO470_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6253277.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(4845..4970)
                     /gene="ecnA"
                     /locus_tag="OO470_004241"
     CDS             complement(4845..4970)
                     /gene="ecnA"
                     /locus_tag="OO470_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6253278.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(5022..5588)
                     /gene="efp"
                     /locus_tag="OO470_004242"
     CDS             complement(5022..5588)
                     /gene="efp"
                     /locus_tag="OO470_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6253279.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT         1434 a         1472 c         1468 g         1310 t
ORIGIN      
        1 gttaaagaac cgattccggt acgtccgact gcacactaca ccatgggcgg tatcgaaacc
       61 gatcagaact gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt
      121 ggtctgcacg gtgcaaaccg tctgggctcc aactccctgg cggaactggt ggtcttcggc
      181 cgtctggccg gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg
      241 gcaattgaag cgcaggcagc tggcgttgaa caacgtctga aagatctagt taaccaggat
      301 ggcggcgaaa actgggcgaa gatccgcgac gaaatgggcc tggcaatgga agaaggttgc
      361 ggtatctacc gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag
      421 gaacgcttca agcgcgtgcg catcaccgac acctccagcg tgttcaacac cgacctgctc
      481 tacaccattg aactgagcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg
      541 gcacgtaaag agtcccgcgg cgcacaccag cgtctggacg aaggttgcac cgagcgtgac
      601 gacgtcaact ttctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg
      661 gagtacagcg acgtgaagat tactacgctg ccgccagcta aacgcgttta cggtggcgaa
      721 gcggatgcag ccgataaggc ggaagcagcc aataataagg agaaggcgaa tggctgagat
      781 gaaaaacctg aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag
      841 cgcattctat gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat
      901 caaagacaac ctggcaccgg acctgagtta ccgctggtcc tgccgtatgg cgatttgtgg
      961 ttcctgcggc atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga
     1021 ttacaccgac ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt
     1081 ggtcgatatg acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa
     1141 ctcccgcacc gcggatcagg gtactaacat ccagaccccg gcgcagatgg caaagtatca
     1201 ccagttctcc ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct
     1261 gaacccagag ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag
     1321 ccgcgaccac ggtaagaagg agcgtatggc gcagttgaac agccagaacg gtgtatggag
     1381 ctgtactttc gtgggctatt gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc
     1441 cattcagcag ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg
     1501 ctaaggagtg caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct
     1561 ggtggaaaaa attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcggttccgg
     1621 ctgtgtggtt cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag
     1681 cctgggcggg attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca
     1741 ctctgacggc agctctgctg cacaccaaaa cctggtttga actggcaccg aaagcggcca
     1801 atatcattgt aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg
     1861 taactgtggt tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag
     1921 atgattaatc caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc
     1981 ggtggtatgt ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg tattctgctg
     2041 ccactggggc tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag
     2101 agcttcattg gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta
     2161 caccgtatgc accacgcgat gcacgatctg aaaatccacg tgcctgcggg caaatgggtt
     2221 ttctacggtc tggctgctat cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa
     2281 cgcatcgcca atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc
     2341 ttatgttcaa aacgacgctc tgcgccttat taattaccgc ctcttactcc acatttgctg
     2401 cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa
     2461 agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg
     2521 gctatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt
     2581 cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca
     2641 agttaagcga tcccgcaaca aaatactggc ctgaacttac cgctaaacag tggaatggga
     2701 tcacactatt acatcttgcg acctacaccg ctggcggcct gccattgcag gtgccggatg
     2761 aagtgaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc
     2821 caggaacaca acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga
     2881 agccgtctgg tttgagtttt gagcaggcga tgcaaactcg tgtcttccag ccactcaaac
     2941 tcaaccatac gtggattaat gtacctcccc cagaagaaaa gaattacgcc tggggatatc
     3001 gcgaaggtaa ggcagtgcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga
     3061 agtcgaccat tgaagatatg gcccgctggg tgcaaagcaa tttacccccc cttgatatca
     3121 atgagaaaac gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg
     3181 atatgtatca gggcctgggc tgggaaatgc tggactggcc ggtcaatcct gacagcatca
     3241 ttaacggcag tgacaataaa attgcactgg cagcacgccc ggtaaaagcg attaagcccc
     3301 caactcctgc agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta
     3361 gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact
     3421 atcccaatcc agcgagagtc gccgccgcct gtcagattct caacgctcta cagtaaaatt
     3481 tcatcgggtc cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag
     3541 aaaaaaacca gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct
     3601 gcatttctgg tcgttgcctg tagttttcct acgccgccgc gtggcgtgac cgtagtgaat
     3661 aatttcgacg ccaaacgtta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt
     3721 tttgaacgtg gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg
     3781 aatgtcatta ataaaggcta taaccctgac agaggaatgt ggcagcagag tgaagggaaa
     3841 gcgtacttta ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc
     3901 tatggcggtt ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc
     3961 ccggaccgcg actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa
     4021 caggagatgc tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta
     4081 cagcagcccg gtagttagtg agtgctgagt ttcagaccaa taatccccaa tacgattaac
     4141 gccagactcg ccagacgcat cgggttagcg gactcaccga gcagcacaat gccggtgatg
     4201 gccgcgccga ctgcgccaat acccgtccac acggcataag ccgtccctac cggtaacgat
     4261 ttcatcgccc aggcaagtag cgccatactg actatcatcg ccgtcacggt aataacactc
     4321 ggcgtcaaac ggctaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc
     4381 agaccagcaa taactaagat aatccaggac atatcaggct ccggaaattt ggggccgtcc
     4441 ccggtgaaaa gaggcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg
     4501 ccaggtggca ggggaatttc aactattacc tttatgacga tcgcagaaaa aacaaacggc
     4561 acgacactgc tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg cgccagaaat
     4621 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
     4681 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
     4741 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
     4801 caaaaatgat ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
     4861 gagatggagt taccgagatg tttgatgtct tcgccgaaac cgcaagcggt gttacagccc
     4921 gtgagcagcg tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
     4981 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
     5041 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaact
     5101 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
     5161 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
     5221 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagca
     5281 ttgtcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
     5341 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
     5401 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
     5461 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
     5521 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
     5581 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
     5641 tgtaacccta aataccccat ccagagaaga ttggttaacg caac
//
LOCUS       DAJTUN010000440         4936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.guided.186, whole genome shotgun sequence.
ACCESSION   DAJTUN010000440 DAJTUN010000000
VERSION     DAJTUN010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.guided.186"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            7..630
                     /locus_tag="OO470_004243"
     CDS             7..630
                     /locus_tag="OO470_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6253280.1"
                     /translation="MSDISRVKILSALMDGRAWTATELSSVANISASTASSHLSKLLD
                     CQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHGVHAKVSTPVHLRKARTCYDHL
                     AGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSER
                     RFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(608..1231)
                     /gene="tetR(B)"
                     /locus_tag="OO470_004244"
     CDS             complement(608..1231)
                     /gene="tetR(B)"
                     /locus_tag="OO470_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6253281.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1313..2518
                     /gene="tet(B)"
                     /locus_tag="OO470_004245"
     CDS             1313..2518
                     /gene="tet(B)"
                     /locus_tag="OO470_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6253282.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2631..3224)
                     /gene="tetC"
                     /locus_tag="OO470_004246"
     CDS             complement(2631..3224)
                     /gene="tetC"
                     /locus_tag="OO470_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6253283.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3357..3728
                     /locus_tag="OO470_004247"
     CDS             3357..3728
                     /locus_tag="OO470_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6253284.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3738..>4936)
                     /locus_tag="OO470_004248"
     CDS             complement(3738..>4936)
                     /locus_tag="OO470_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR6253285.1"
                     /translation="HKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRNL
                     PTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKR
                     LMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGF
                     KVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQ
                     LVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVH
                     AQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHG
                     YALGKL"
BASE COUNT         1317 a          933 c         1069 g         1617 t
ORIGIN      
        1 gcggctatgt ctgacatttc acgagttaaa atactcagtg ctttgatgga tgggcgagct
       61 tggacggcca ctgagctaag ttctgtggcg aatatatcag cttcaacggc gagcagtcat
      121 ttatctaaat tattagattg ccagctaatc acagtagtag ctcaaggcaa gcatcgttat
      181 tttcggctag caggaaaaga tattgctgaa ttgatggaaa gtatgatggg gatctcctta
      241 aaccatggcg tacatgccaa agtttccacg ccagtgcatt tacgaaaagc acgtacttgc
      301 tatgatcatt tagctggcga agttgccgtt aagatctatg attccctttg tcaacagcaa
      361 tggatcactg aaaatggttc aatgatcaca ttaagtggta ttcaatattt tcatgaaatg
      421 ggaattgacg ttccttccaa acattcacgt aaaatctgtt gtgcgtgttt agattggagt
      481 gaacgccgtt tccatttagg tgggtacgtt ggagccgcat tattttcgct ttatgaatct
      541 aaagggtggt taactcgaca tcttggttac cgtgaagtta ccatcacgga aaaaggttat
      601 gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt
      661 caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc
      721 gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat
      781 gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata
      841 atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt
      901 catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta
      961 gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt
     1021 ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca
     1081 aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg
     1141 cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt
     1201 taatcacttt gcttttatct aatctagaca tcattaattc ctaatttttg ttgacactct
     1261 atcattgata gagttatttt accactccct atcagtgata gagaaaagtg aaatgaatag
     1321 ttcgacaaag atcgcattgg taattacgtt actcgatgcc atggggattg gccttatcat
     1381 gccagtcttg ccaacgttat tacgtgaatt tattgcttcg gaagatatcg ctaaccactt
     1441 tggcgtattg cttgcacttt atgcgttaat gcaggttatc tttgctcctt ggcttggaaa
     1501 aatgtctgac cgatttggtc ggcgcccagt gctgttgttg tcattaatag gcgcatcgct
     1561 ggattactta ttgctggctt tttcaagtgc gctttggatg ctgtatttag gccgtttgct
     1621 ttcagggatc acaggagcta ctggggctgt cgcggcatcg gtcattgccg ataccacctc
     1681 agcttctcaa cgcgtgaagt ggttcggttg gttaggggca agttttgggc ttggtttaat
     1741 agcggggcct attattggtg gttttgcagg agagatttca ccgcatagtc ccttttttat
     1801 cgctgcgttg ctaaatattg tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa
     1861 aaatacacgt gataatacag ataccgaagt aggggttgag acgcaatcga attcggtata
     1921 catcacttta tttaaaacga tgcccatttt gttgattatt tatttttcag cgcaattgat
     1981 aggccaaatt cccgcaacgg tgtgggtgct atttaccgaa aatcgttttg gatggaatag
     2041 catgatggtt ggcttttcat tagcgggtct tggtctttta cactcagtat tccaagcctt
     2101 tgtggcagga agaatagcca ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat
     2161 tgcagatagt agtgcatttg cctttttagc gtttatatct gaaggttggt tagttttccc
     2221 tgttttaatt ttattggctg gtggtgggat cgctttacct gcattacagg gagtgatgtc
     2281 tatccaaaca aagagtcatc agcaaggtgc tttacaggga ttattggtga gccttaccaa
     2341 tgcaaccggt gttattggcc cattactgtt tgctgttatt tataatcatt cactaccaat
     2401 ttgggatggc tggatttgga ttattggttt agcgttttac tgtattatta tcctgctatc
     2461 gatgaccttc atgttaaccc ctcaagctca ggggagtaaa caggagacaa gtgcttagtt
     2521 atttcgtcac caaatgatgt tattccgcga aatataatga ccctcttgat aacccaagag
     2581 ggcatttttt acgataaaga agatttagct tcaaataaaa cctatctatt ttatttatct
     2641 ttcaagctca ataaaaagcc gcggtaaata gcaataaatt ggcctttttt atcggcaagc
     2701 tcttttaggt ttttcgcatg tattgcgata tgcataaacc agccattgag taagttttta
     2761 agcacatcat catcataagc tttaagttgg ttctcttgga tcaatttgct gacaatggcg
     2821 tttaccttac cagtaatgta ttcaaggcta attttttcaa gttcattcca accaatgata
     2881 ggcatcactt cttggatagg gataaggttt ttattattat caataatata atcaagataa
     2941 tgttcaaata tactttctaa ggcagaccaa ccatttgtta aatcagtttt tgttgtgatg
     3001 taggcatcaa tcataattaa ttgctgctta taacaggcac tgagtaattg ttttttattt
     3061 ttaaagtgat gataaaaggc acctttggtc accaacgctt ttcccgagat ctcatctatt
     3121 gaaacagctt gatagccttt ttcaacaaac aatattcgtg ctgagttaac cagtgattga
     3181 taggtactct taaaattttc ttgttgatga tttttatttt ccatgataga tttaaaataa
     3241 cataccgtca gtatgtttat ggtatcatga tgatgtggtc gtgacaatct taagaacatt
     3301 taggttattt tatgtatatt gaacagcatt ctcgctatca aaataaagct aataacatcc
     3361 aattaagata tgatgataag cagtttcata caacggttat caaagatgtt ctattatgga
     3421 ttgaacataa tttagatcag tctttactgc ttgatgatgt ggcgaataaa gcgggttata
     3481 ccaagtggta ttttcagcgg ctgttcaaaa aagtaacagg ggtcacactg gctagctata
     3541 ttcgtgctcg tcgtttgacg aaagcggctg ttgagttgag gttgacgaaa aaaactatcc
     3601 ttgagatcgc attaaaatat caatttgatt cccaacaatc ttttacacgt cgatttaagt
     3661 acatttttaa ggttacacca agttattatc ggcgtaataa attatgggaa ttggaggcaa
     3721 tgcactgaga gatcccctca taatttcccc aaagcgtaac catgtgtgaa taaattttga
     3781 gctagtaggg ttgcagccac gagtaagtct tcccttgtta ttgtgtagcc agaatgccgc
     3841 aaaacttcca tgcctaagcg aactgttgag agtacgtttc gatttctgac tgtgttagcc
     3901 tggaagtgct tgtcccaacc ttgtttctga gcatgaacgc ccgcaagcca acatgttagt
     3961 tgaagcatca gggcgattag cagcatgata tcaaaacgct ctgagctgct cgttcggcta
     4021 tggcgtaggc ctagtccgta ggcaggactt ttcaagtctc ggaaggtttc ttcaatctgc
     4081 attcgcttcg aatagatatt aacaagttgt ttgggtgttc gaatttcaac aggtaagtta
     4141 gttgctagaa cccatggctc ctttgccgac gctgagtaga ttttaggtga cgggtggtga
     4201 caatgagtcc gtgtcgagcg ctgatttttt cggcctttag agcgagattt atacaataga
     4261 atttggcatg agattggatt gcttttagtc agcctcttat agcctaaagt ctttgagtga
     4321 ctagatgaca tatcatgtaa gttgctgata ggtttccagt tttccgctcc taggtctgca
     4381 tattgtactt ttcctcttac tcgacttaac cagtaccaac ccagcttctc aacggattta
     4441 taccatggca ctttaaagcc agcatcactg acaatgagcg gtgtggtgtt actcggtaga
     4501 atgctcgcaa ggtcggctag aaattggtca tgagctttct ttgaacattg ctctgaaagc
     4561 gggaacgctt tctcataaag agtaacagaa cgaccgtgta gtgcgactga agctcgcaat
     4621 accataagtc gtttttgctc acgaatatca gaccagtcaa caagtacaat gggcatcgta
     4681 ttgcccgaac agataaagct agcatgccaa cggtatacag cgagtcgctc tttgtggagg
     4741 tgacgattac ctaacaatcg gtcgattcgt ttgatgttat gttttgttct cgctttggtt
     4801 ggcaggttac ggccaagttc ggtaagagtg agagttttac agtcaagtaa tgcgtggcaa
     4861 gccaacgtta agctgttgag tcgttttaag tgtaattcgg ggtaatgact ccaacttact
     4921 gatagtgttt tatgtt
//
LOCUS       DAJTUN010000441         3593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.guided.223, whole genome shotgun sequence.
ACCESSION   DAJTUN010000441 DAJTUN010000000
VERSION     DAJTUN010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.guided.223"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            727..1542
                     /gene="sul2"
                     /locus_tag="OO470_004249"
     CDS             727..1542
                     /gene="sul2"
                     /locus_tag="OO470_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6253286.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1603..2406
                     /locus_tag="OO470_004250"
                     /note="aph(3'')-Ib"
     CDS             1603..2406
                     /locus_tag="OO470_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6253287.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2406..3242
                     /locus_tag="OO470_004251"
     CDS             2406..3242
                     /locus_tag="OO470_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6253288.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3349..3593)
                     /locus_tag="OO470_004252"
                     /pseudo
     CDS             complement(3349..3593)
                     /locus_tag="OO470_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          807 a          973 c          963 g          850 t
ORIGIN      
        1 aactcatggt ctgttattta tcgtaaaaat tccggagaag atatcaatat cacttccctg
       61 acgtttaaaa atagtcttct ggcagcccgg acactgatgg tgcccgaaaa ctatatgata
      121 tgcatactca gaaacgggga gagggtcaga cgatgggaca gagaaatact tgcaggctca
      181 aacagatggt acaagtgttc tcccgataat ttcgaaatac tgggtaagct ccccataata
      241 aataaagtca caaaactgat aaaatcataa tctacggatt ttaatcagtt aagctaaatc
      301 aggatgaacg ctttgattat gaattataca tgaaaacaaa tatgggcagc acaaaaaatg
      361 gtgccagggt tccccacgtt cgtaatagat tcacttctat ggatattggt atgaattccc
      421 agatcccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
      481 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
      541 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
      601 gatgtatgct attctgctcc tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg
      661 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      721 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      781 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      841 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      901 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      961 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
     1021 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
     1081 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1141 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1201 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1261 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1321 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1381 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1441 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1501 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1561 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1621 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1681 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1741 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1801 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1861 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1921 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     1981 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     2041 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     2101 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     2161 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     2221 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     2281 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     2341 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     2401 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     2461 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     2521 tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc
     2581 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     2641 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     2701 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     2761 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     2821 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     2881 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     2941 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     3001 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     3061 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     3121 tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag
     3181 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     3241 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     3301 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgaccccaa acaggaaacg
     3361 cagctgaaac gggaagctca acacccactg acgcatgggt tgttcaggca gtacttcatc
     3421 aaccagcaag gcggcacttt cggccatccg ccgcgcccca cagctcgggc agaaaccgcg
     3481 acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag
     3541 aaagccatgc tccagccgcc cgcattggag aaattcttca aattcccgtt gca
//
LOCUS       DAJTUN010000442         2642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.guided.239, whole genome shotgun sequence.
ACCESSION   DAJTUN010000442 DAJTUN010000000
VERSION     DAJTUN010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.guided.239"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            166..624
                     /gene="gpt"
                     /locus_tag="OO470_004253"
     CDS             166..624
                     /gene="gpt"
                     /locus_tag="OO470_004253"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6253289.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            716..1960
                     /gene="frsA"
                     /locus_tag="OO470_004254"
     CDS             716..1960
                     /gene="frsA"
                     /locus_tag="OO470_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6253290.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2018..2419
                     /gene="crl"
                     /locus_tag="OO470_004255"
     CDS             2018..2419
                     /gene="crl"
                     /locus_tag="OO470_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6253291.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(2458..>2642)
                     /locus_tag="OO470_004256"
     CDS             complement(2458..>2642)
                     /locus_tag="OO470_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6253292.1"
                     /translation="KDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKL
                     NINNDDTVAVGMTYQF"
BASE COUNT          647 a          639 c          686 g          670 t
ORIGIN      
        1 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
       61 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
      121 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
      181 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
      241 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggggcgtta
      301 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
      361 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
      421 gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca
      481 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
      541 gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc
      601 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cattgccggg cgttgttctt
      661 tttaacttca ggcaggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
      721 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
      781 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc ttgatggtaa
      841 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggtattga
      901 cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg aacgtaccga
      961 cgatgattta tacgataccg tgattggcta ccgtggtggc aactggattt atgagtgggc
     1021 cacccaggcg atggtgtggc aacaaaaagc ctgtacggaa gaagatccga aactcagtgg
     1081 tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg
     1141 agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag aggccgccca
     1201 gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac
     1261 cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg
     1321 tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg
     1381 cattgcgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac
     1441 ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt
     1501 ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct
     1561 ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac
     1621 cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc
     1681 gagtcgtttg gggatgcatg atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta
     1741 ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca acgccaatgt tatcaggcta
     1801 ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc
     1861 tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct
     1921 tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca
     1981 atttggtaaa acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc
     2041 gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg aaggtaagtg
     2101 cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc
     2161 ggaagtgcgt gaattctggg gctggtggat ggagctggaa gcgcaggaat cccgttttac
     2221 ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa
     2281 agatactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg
     2341 tgaactgctg acgacgctga atctgaagct ggaaccggcg aatgattttc gtgatgaacc
     2401 ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca atattcatta
     2461 aaactgatac gtcatcccaa ccgcgacagt atcatcatta ttaatattca gcttattatc
     2521 gctatccagt tggttgattt tataatcaac aaacgctgac atatttttgt tgaaataata
     2581 cgtagcaccg acgtcgatat aattgaccag atcttcatca ccgatgccct caatatcttt
     2641 cc
//
LOCUS       DAJTUN010000443         2642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600906
            SAMEA8900453-rid15817333.guided.240, whole genome shotgun sequence.
ACCESSION   DAJTUN010000443 DAJTUN010000000
VERSION     DAJTUN010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900453
            Sequence Read Archive: ERR6004822
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477736.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:20:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,896
            CDSs (with protein)               :: 3,896
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 45
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 151 of 310
            Pseudo Genes (incomplete)         :: 46 of 310
            Pseudo Genes (internal stop)      :: 136 of 310
            Pseudo Genes (multiple problems)  :: 23 of 310
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900453-rid15817333.guided.240"
                     /serovar="S. flexneri 1b"
                     /isolate="600906"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-09-08"
     gene            166..624
                     /gene="gpt"
                     /locus_tag="OO470_004257"
     CDS             166..624
                     /gene="gpt"
                     /locus_tag="OO470_004257"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6253293.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            716..1960
                     /gene="frsA"
                     /locus_tag="OO470_004258"
     CDS             716..1960
                     /gene="frsA"
                     /locus_tag="OO470_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6253294.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2018..2419
                     /gene="crl"
                     /locus_tag="OO470_004259"
     CDS             2018..2419
                     /gene="crl"
                     /locus_tag="OO470_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6253295.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(2458..>2642)
                     /locus_tag="OO470_004260"
     CDS             complement(2458..>2642)
                     /locus_tag="OO470_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6253296.1"
                     /translation="KDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKL
                     NINNDDTVAVGMTYQF"
BASE COUNT          646 a          639 c          687 g          670 t
ORIGIN      
        1 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
       61 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
      121 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
      181 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
      241 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggggcgtta
      301 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
      361 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
      421 gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca
      481 aaagcgcact ttgtcaccat cttcgcaaag ccggctggtc gtccgctggt tgatgactat
      541 gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc
      601 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cattgccggg cgttgttctt
      661 tttaacttca ggcaggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
      721 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
      781 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc ttgatggtaa
      841 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggtattga
      901 cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg aacgtaccga
      961 cgatgattta tacgataccg tgattggcta ccgtggtggc aactggattt atgagtgggc
     1021 cacccaggcg atggtgtggc aacaaaaagc ctgtacggaa gaagatccga aactcagtgg
     1081 tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg
     1141 agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag aggccgccca
     1201 gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac
     1261 cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg
     1321 tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg
     1381 cattgcgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac
     1441 ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt
     1501 ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct
     1561 ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac
     1621 cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc
     1681 gagtcgtttg gggatgcatg atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta
     1741 ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca acgccaatgt tatcaggcta
     1801 ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc
     1861 tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct
     1921 tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca
     1981 atttggtaaa acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc
     2041 gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg aaggtaagtg
     2101 cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc
     2161 ggaagtgcgt gaattctggg gctggtggat ggagctggaa gcgcaggaat cccgttttac
     2221 ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa
     2281 agatactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg
     2341 tgaactgctg acgacgctga atctgaagct ggaaccggcg aatgattttc gtgatgaacc
     2401 ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca atattcatta
     2461 aaactgatac gtcatcccaa ccgcgacagt atcatcatta ttaatattca gcttattatc
     2521 gctatccagt tggttgattt tataatcaac aaacgctgac atatttttgt tgaaataata
     2581 cgtagcaccg acgtcgatat aattgaccag atcttcatca ccgatgccct caatatcttt
     2641 cc
//
